erythrocyte aging

Summary

Summary: The senescence of RED BLOOD CELLS. Lacking the organelles that make protein synthesis possible, the mature erythrocyte is incapable of self-repair, reproduction, and carrying out certain functions performed by other cells. This limits the average life span of an erythrocyte to 120 days.

Top Publications

  1. ncbi Storage-related changes in erythrocyte band 3: not a case for the Diego blood group antigens
    G J C G M Bosman
    Department of Biochemistry, University Medical Centre Nijmegen, NL 6500 HB Nijmegen, The Netherlands
    Cell Mol Biol (Noisy-le-grand) 51:195-200. 2005
  2. ncbi Accelerated senescence of human erythrocytes cultured with Plasmodium falciparum
    Fausta Omodeo-Salè
    Istituto di Fisiologia Generale e Chimica Biologica Giovanni Esposito, Facolta di Farmacia, Via Trentacoste 2, 20134 Milano, Italy
    Blood 102:705-11. 2003
  3. ncbi Red blood cell senescence and neocytolysis in humans after high altitude acclimatization
    Angela Risso
    M A T I Microgravity, Aging, Training, Immobility Centre of Excellence, University of Udine, P le M Kolbe 4, I 33100 Udine, Italy
    Blood Cells Mol Dis 38:83-92. 2007
  4. doi Erythrocyte vesiculation: a self-protective mechanism?
    Frans L A Willekens
    Department of Clinical Chemistry, Rijnstate Hospital, Arnhem, The Netherlands
    Br J Haematol 141:549-56. 2008
  5. ncbi The mechanism of loss of CR1 during maturation of erythrocytes is different between factor I deficient patients and healthy donors
    Sylvie Miot
    Department of Research, University Hospital Basel, Basel, Switzerland
    Blood Cells Mol Dis 29:200-12. 2002
  6. ncbi Complement-mediated clearance of erythrocytes: mechanism and delineation of the regulatory roles of Crry and DAF. Decay-accelerating factor
    Hector Molina
    Center for Experimental Therapeutics, Department of Pharmacology, University of Pennsylvania School of Medicine, Philadelphia 19104, USA
    Blood 100:4544-9. 2002
  7. ncbi Senescent erythrocytes: modification of rheologic properties, antigenic expression and interaction with monocytes
    A Racca
    Laboratorio Inmunohematología, Hemorreología e Inmunogenética, Facultad de Ciencias Bioquimicas y Farmaceuticas, Universidad Nacional de Rosario, Argentina
    Medicina (B Aires) 59:33-7. 1999
  8. ncbi Erythrocyte aging: a more than superficial resemblance to apoptosis?
    Giel J C G M Bosman
    Department of Biochemistry, Radboud University Nijmegen Medical Center, Nijmegen Center for Molecular Life Sciences, Nijmegen, The Netherlands
    Cell Physiol Biochem 16:1-8. 2005
  9. doi Comparative proteomics of erythrocyte aging in vivo and in vitro
    G J C G M Bosman
    Department of Biochemistry, Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen Medical Centre, The Netherlands
    J Proteomics 73:396-402. 2010
  10. ncbi Acidic and neutral sialidase in the erythrocyte membrane of type 2 diabetic patients
    Bruno Venerando
    Department of Medical Chemistry and Biochemistry, School of Medicine, University of Milan, LITA, Via Fratelli Cervi, 93 20090 Segrate, Milan, Italy
    Blood 99:1064-70. 2002

Research Grants

Detail Information

Publications131 found, 100 shown here

  1. ncbi Storage-related changes in erythrocyte band 3: not a case for the Diego blood group antigens
    G J C G M Bosman
    Department of Biochemistry, University Medical Centre Nijmegen, NL 6500 HB Nijmegen, The Netherlands
    Cell Mol Biol (Noisy-le-grand) 51:195-200. 2005
    ..This makes it unlikely that immunological removal of erythrocytes after transfusion is mediated by reactions involving the Diego blood group system...
  2. ncbi Accelerated senescence of human erythrocytes cultured with Plasmodium falciparum
    Fausta Omodeo-Salè
    Istituto di Fisiologia Generale e Chimica Biologica Giovanni Esposito, Facolta di Farmacia, Via Trentacoste 2, 20134 Milano, Italy
    Blood 102:705-11. 2003
    ..In summary, we demonstrated that membrane damage leading to accelerated senescence of both infected and uninfected erythrocytes will likely contribute to malaria anemia...
  3. ncbi Red blood cell senescence and neocytolysis in humans after high altitude acclimatization
    Angela Risso
    M A T I Microgravity, Aging, Training, Immobility Centre of Excellence, University of Udine, P le M Kolbe 4, I 33100 Udine, Italy
    Blood Cells Mol Dis 38:83-92. 2007
    ..50% (+/-7) to 97.90% (+/-2.00)). Furthermore, the remaining young and middle-aged RBCs had acquired a senescent-like phenotype, which may account for their increased susceptibility to phagocytosis...
  4. doi Erythrocyte vesiculation: a self-protective mechanism?
    Frans L A Willekens
    Department of Clinical Chemistry, Rijnstate Hospital, Arnhem, The Netherlands
    Br J Haematol 141:549-56. 2008
    ..Consequently, these same removal molecules mediate the rapid removal of erythrocyte-derived vesicles from the circulation...
  5. ncbi The mechanism of loss of CR1 during maturation of erythrocytes is different between factor I deficient patients and healthy donors
    Sylvie Miot
    Department of Research, University Hospital Basel, Basel, Switzerland
    Blood Cells Mol Dis 29:200-12. 2002
    ..These data suggest that cell surface domains rich in CR1, but not in DAF, are specifically lost in factor I deficiency...
  6. ncbi Complement-mediated clearance of erythrocytes: mechanism and delineation of the regulatory roles of Crry and DAF. Decay-accelerating factor
    Hector Molina
    Center for Experimental Therapeutics, Department of Pharmacology, University of Pennsylvania School of Medicine, Philadelphia 19104, USA
    Blood 100:4544-9. 2002
    ..These results shed new light on the relative activities of Crry and DAF and underscore the critical roles of membrane C3 regulators in preventing spontaneous and antibody-induced erythrocyte damage in vivo...
  7. ncbi Senescent erythrocytes: modification of rheologic properties, antigenic expression and interaction with monocytes
    A Racca
    Laboratorio Inmunohematología, Hemorreología e Inmunogenética, Facultad de Ciencias Bioquimicas y Farmaceuticas, Universidad Nacional de Rosario, Argentina
    Medicina (B Aires) 59:33-7. 1999
    ..01). The significant modifications in the biomechanic properties of senescent red blood cell membrane and the loss of antigenic expression could lead to physiological phagocytosis...
  8. ncbi Erythrocyte aging: a more than superficial resemblance to apoptosis?
    Giel J C G M Bosman
    Department of Biochemistry, Radboud University Nijmegen Medical Center, Nijmegen Center for Molecular Life Sciences, Nijmegen, The Netherlands
    Cell Physiol Biochem 16:1-8. 2005
    In physiological circumstances, erythrocyte aging leads to binding of autologous IgG followed by recognition and removal through phagocytosis, mainly by Kupffer cells in the liver...
  9. doi Comparative proteomics of erythrocyte aging in vivo and in vitro
    G J C G M Bosman
    Department of Biochemistry, Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen Medical Centre, The Netherlands
    J Proteomics 73:396-402. 2010
    ..maintain erythrocyte homeostasis; (2) open new roads to elucidate the processes that regulate physiological erythrocyte aging and removal, and thereby; (3) provide the foundation for rational interventions to prevent untimely ..
  10. ncbi Acidic and neutral sialidase in the erythrocyte membrane of type 2 diabetic patients
    Bruno Venerando
    Department of Medical Chemistry and Biochemistry, School of Medicine, University of Milan, LITA, Via Fratelli Cervi, 93 20090 Segrate, Milan, Italy
    Blood 99:1064-70. 2002
    ..It has been suggested that the expression of the neutral enzyme requires some activation mechanism that is impaired in diabetes...
  11. pmc Quantitative analysis of mechanisms that govern red blood cell age structure and dynamics during anaemia
    Nicholas J Savill
    Centre for Infectious Diseases, Institute of Immunology and Infection Research, University of Edinburgh, Edinburgh, United Kingdom
    PLoS Comput Biol 5:e1000416. 2009
    ..We also provide evidence of large diurnal oscillations in serum erythropoietin levels during anaemia...
  12. ncbi A detailed study of time-dependent changes in human red blood cells: from reticulocyte maturation to erythrocyte senescence
    Sean C Gifford
    Visual and Circulatory Biophysics Laboratory, Department of Biomedical Engineering, Boston University, Boston, MA, USA
    Br J Haematol 135:395-404. 2006
    ..15%) and a model describing the complete time-course of diminishing mean RBC area and Hb is proposed. The relationship between the losses of Hb and area, and their possible influence on red cell lifespan, are discussed...
  13. ncbi Band 3/complement-mediated recognition and removal of normally senescent and pathological human erythrocytes
    Paolo Arese
    Department of Genetics, Biology and Biochemistry, University of Torino Medical School, Torino
    Cell Physiol Biochem 16:133-46. 2005
    ..falciparum malaria, may lead to massive and rapid destruction of RBCs by a mechanism comparable to a dramatic, time-compressed enhancement of normal RBC senescence...
  14. ncbi Erythrocyte aging in neurodegenerative disorders
    L de Franceschi
    Department of Clinical and Experimental Medicine, Section of Internal Medicine, University of Verona, Policlinico GB Rossi, P le L Scuro 10, 37134 Verona, Italy
    Cell Mol Biol (Noisy-le-grand) 50:179-85. 2004
    ..We have reviewed the mechanism(s) of the loss of red cell membrane stability and of the precocious red cell aging in neurodegenerative disorders...
  15. ncbi Splenectomy prolongs in vivo survival of erythrocytes differently in spectrin/ankyrin- and band 3-deficient hereditary spherocytosis
    Ramune Reliene
    Institute of Biochemistry, Swiss Federal Institute of Technology, ETH Hönggerberg HPM D14 2, CH 8093 Zurich, Switzerland
    Blood 100:2208-15. 2002
    ....
  16. ncbi The in vitro quality of washed, prestorage leucocyte-depleted red blood cell concentrates
    V Weisbach
    Department of Transfusion Medicine and Haemostaseology, Friedrich Alexander Universitat Erlangen Nurnberg, Erlangen, Germany
    Vox Sang 87:19-26. 2004
    ..No data are currently available on the quality of washed prestorage leucocyte-depleted red blood cell concentrates (RCCs)...
  17. ncbi The 24-hour posttransfusion survival of baboon red blood cells preserved in citrate phosphate dextrose/ ADSOL (CPD/AS-1) for 49 days
    C Robert Valeri
    Naval Blood Research Laboratory, Boston University School of Medicine, 615 Albany Street, Boston, Massachusetts 02118, USA
    Contemp Top Lab Anim Sci 44:38-40. 2005
    ..Further storage for 42 and 49 days resulted in lower values for baboon RBCs compared with human RBCs...
  18. ncbi [Erythrophagocytosis and recycling of heme iron in normal and pathological conditions; regulation by hepcidin]
    C Beaumont
    Inserm U656, Faculte Xavier Bichat, Paris
    Transfus Clin Biol 12:123-30. 2005
    ..Perturbations in erythrophagocytosis play a physiopathological role in several diseases, including hemochromatosis, anemia of chronic disorders and thalassemia...
  19. ncbi Interlaboratory comparison of red-cell ATP, 2,3-diphosphoglycerate and haemolysis measurements
    J R Hess
    University of Maryland Medical School, Baltimore, MD 21201, USA
    Vox Sang 89:44-8. 2005
    ..The reproducibility of RBC ATP, DPG and haemolysis measurements between centres was investigated...
  20. ncbi Production of pathogen-inactivated RBC concentrates using PEN110 chemistry: a Phase I clinical study
    James P AuBuchon
    Cell Labeling Laboratory, Department of Pathology, Dartmouth Hitchcock Medical Center, Lebanon, New Hampshire, USA
    Transfusion 42:146-52. 2002
    ..Despite extensive reductions in risk, donor selection and testing cannot eliminate pathogen transmission. The objective of this study was to evaluate clinically the viability of pathogen-inactivated RBCs...
  21. ncbi Survival of the fittest?--survival of stored red blood cells after transfusion
    M Luten
    Department of Research and Development, Sanquin Blood Bank Southeast Region, P O Box 1013, 6501 BA Nijmegen, The Netherlands
    Cell Mol Biol (Noisy-le-grand) 50:197-203. 2004
    ..1% for the measurement of survival in vivo based on antigenic differences. This technique has various advantages compared with the 'classical' 51Cr survival method...
  22. ncbi Phosphotyrosine phosphatases acting on band 3 in human erythrocytes of different age: PTP1B processing during cell ageing
    Annarita Ciana
    Universita di Pavia, Dipartimento di Biochimica A Castellani, Sezione di Scienze, via Bassi, 21, 27100 Pavia, Italy
    Bioelectrochemistry 62:169-73. 2004
    ....
  23. ncbi Mapping of senescent cell antigen on brain anion exchanger protein (AE) isoforms using HPLC and fast atom bombardment ionization mass spectrometry (FAB-MS)
    Marguerite M Kay
    International Foundation for Biomedical Aging Research, PO Box 1887, Temple, TX 76503 1887, USA
    J Mol Recognit 17:33-40. 2004
    ..This provides confirmation that SCA is on non-erythroid cell types. Implications of these studies to the generation of neoantigens by oxidation and their recognition by autoantibodies to them are discussed...
  24. ncbi Band 3 and its alterations in health and disease
    M M Kay
    Department of Neurology, University of Geneva Hospital, Micheli du Crest 24, CH 1211, Geneva, Switzerland
    Cell Mol Biol (Noisy-le-grand) 50:117-38. 2004
    ..Mutations in other genes can cause changes in band 3. An example is sickle cell anemia where the increased oxidation causes accelerated aging of band 3 and increased IgG binding and cellular removal...
  25. ncbi The red cell revisited--matters of life and death
    J M Werre
    Department of Blood Transfusion and Transplantation Immunology, University Medical Center, Nijmegen, The Netherlands
    Cell Mol Biol (Noisy-le-grand) 50:139-45. 2004
    ..Removal of old erythrocytes from the circulation and from donor blood may open new ways into the treatment of both bilirubin and secondary iron overload...
  26. ncbi Oxygen transport in blood at high altitude: role of the hemoglobin-oxygen affinity and impact of the phenomena related to hemoglobin allosterism and red cell function
    Michele Samaja
    Dipartimento di Medicina, Chirurgia e Odontoiatria, Ospedale San Paolo, Universita di Milano, Via di Rudini 8, 20142 Milan, Italy
    Eur J Appl Physiol 90:351-9. 2003
    ....
  27. ncbi Time course of the age-related alterations in stored blood
    Sibel Türkes
    Physics Engineering Department, Faculty of Engineering, Hacettepe University, Beytepe, Ankara 06532, Turkey
    Biophys Chem 105:143-50. 2003
    ..Two exponential curves attributed to the liquid and cellular parts of a given samples were found to be described best in the reduction of MAL-6 spin label in WB, AE and AAE...
  28. ncbi Increased release of free Fe ions in human erythrocytes during aging in the circulation
    Ken Ando
    School of Pharmacy, Tokyo University of Pharmacy and Life Science, 1432 1 Horinouchi, Hachioji, Tokyo 192 0392, Japan
    Free Radic Res 36:1079-84. 2002
    ..The results indicated that free Fe ions were released from erythrocytes during aging process in the circulation. Released free Fe ions would promote oxidative damages of the cells during aging process...
  29. ncbi Characterization of binding and phagocytosis of oxidatively damaged erythrocyte to macrophage
    Hong Sook Ko
    Division of Cardiology, Department of Internal Medicine, College of Medicine, Chung Ang University, Seoul, Korea
    Korean J Intern Med 17:220-6. 2002
    ..However, there are controversies about the kind of receptors and ligands related to the binding. Macrophages lacking class A scavenger receptor show identical binding of oxRBC with wild-type ones...
  30. ncbi Molecular and cellular mechanisms of erythrocyte programmed cell death: impact on blood transfusion
    D Bratosin
    National Institute of Biological Science Research and Development, Bucarest, Rumania
    Vox Sang 83:307-10. 2002
  31. ncbi Phosphatidylserine externalization in sickle red blood cells: associations with cell age, density, and hemoglobin F
    Zahida Yasin
    Vontz Center for Molecular Studies, Hematology Oncology Division ML 0508, Department of Internal Medicine, University of Cincinnati College of Medicine, 3125 Eden Avenue, Cincinnati, OH, USA
    Blood 102:365-70. 2003
    ..These studies indicate that PS externalization in sickle cells may be low level, as observed in many immature cells, or high level, which is associated with dehydration and appears to be more specific for sickle RBCs...
  32. ncbi Experiences in the measurement of RBC-bound IgG as markers of cell age
    Renata Paleari
    Dipartimento di Scienze e Tecnologie Biomediche, Universita degli Studi di Milano, Via Fratelli Cervi 93, 20090 Segrate, Milan, Italy
    Bioelectrochemistry 62:175-9. 2004
    ..A significantly greater number of RBC-bound IgG was measured in splenectomized beta-thalassemia intermedia patients and in subjects with secondary decreases in the C3 complement fraction concentration...
  33. ncbi Human erythroid cells are affected by aluminium. Alteration of membrane band 3 protein
    Daniela Vittori
    Laboratorio de Analisis Biologicos, Departamento de Quimica Biologica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellon II, Piso 4 Ciudad Universitaria, 1428 Buenos Aires, Argentina
    Biochim Biophys Acta 1558:142-50. 2002
    ..The present in vitro results suggest that Al may disturb human erythropoiesis through combined effects on mature erythrocytes and cellular metabolism in late erythroid progenitors...
  34. ncbi Fas-, caspase 8-, and caspase 3-dependent signaling regulates the activity of the aminophospholipid translocase and phosphatidylserine externalization in human erythrocytes
    Debabrata Mandal
    Department of Chemistry, Bose Institute 93 1 Acharya Prafulla Chandra Road, Kolkata 700009, India
    J Biol Chem 280:39460-7. 2005
    ..Our data demonstrate for the first time the novel involvement of Fas/caspase 8/caspase 3-dependent signaling in an enucleated cell leading to PS externalization, a central feature of erythrophagocytosis and erythrocyte biology...
  35. ncbi Hydrogen-peroxide-induced heme degradation in red blood cells: the protective roles of catalase and glutathione peroxidase
    Enika Nagababu
    Molecular Dynamics Section, Gerontology Research Center, National Institute on Aging, National Institutes of Health, 5600 Nathan Shock Drive, Baltimore, MD 21224, USA
    Biochim Biophys Acta 1620:211-7. 2003
    ..The unique role of GSHPX is attributed to its ability to react with hydrogen peroxide generated in close proximity to the red cell membrane in conjunction with the autoxidation of membrane-bound hemoglobin...
  36. ncbi A genetic defect in the binding of protein 4.1 to spectrin in a kindred with hereditary spherocytosis
    L C Wolfe
    N Engl J Med 307:1367-74. 1982
    ..1, but the other functioned normally. Presumable, the nonfunctional spectrin was the product of the autosomal dominant gene responsible for the hereditary spherocytosis in this kindred...
  37. ncbi Storage-dependent remodeling of the red blood cell membrane is associated with increased immunoglobulin G binding, lipid raft rearrangement, and caspase activation
    Anastasios G Kriebardis
    Department of Cell Biology and Biophysics, Faculty of Biology, University of Athens, Panepistimiopolis, Greece
    Transfusion 47:1212-20. 2007
    ..The membrane hosts critical steps in these mechanisms and undergoes widespread remodeling over the storage period...
  38. ncbi Albumin-quercetin combination offers a therapeutic advantage in the prevention of reduced survival of erythrocytes in visceral leishmaniasis
    Gargi Sen
    Indian Association for Cultivation of Science, Kolkata 700032, India
    Blood Cells Mol Dis 39:245-54. 2007
    ..Thus, we speculate that suppression of iron decompartmentalization, with the combination of quercetin and serum albumin might be a new approach in the prevention of reduced survival of erythrocytes in visceral leishmaniasis...
  39. ncbi Effects of physiological parameters on the electrical properties of blood bank stored erythrocyte suspensions
    Mana Sezdi
    Bogazici University, Institute of Biomedical Engineering, Istanbul
    Conf Proc IEEE Eng Med Biol Soc 1:5133-6. 2006
    ..By applying the multi-regression analysis, it is concluded that Ri, Re and Cm are appropriate for modeling Na+, K+, Cl-, pH and ATP during blood bank storage and predicting blood quality...
  40. ncbi Rapid cell corpse clearance by stabilin-2, a membrane phosphatidylserine receptor
    S Y Park
    Department of Biochemistry and Cell Biology, Cell and Matrix Research Institute, School of Medicine, Kyungpook National University, Daegu, Korea
    Cell Death Differ 15:192-201. 2008
    ..These data indicate that stabilin-2 is the first of the membrane PS receptors to provide tethering and tickling signals, and may also be involved in the resolution of inflammation and the prevention of autoimmunity...
  41. ncbi Characterization of the calpastatin defect in erythrocytes from patients with essential hypertension
    S Pontremoli
    Institute of Biological Chemistry, University of Genoa, Italy
    Biochem Biophys Res Commun 157:867-74. 1988
    ....
  42. ncbi Thermal lability of membrane proteins of age separated erythrocytes as studied by electron spin resonance spin label technique
    G Bartosz
    Experimentalphysik III, Gesamthochschule Kassel, Bundesrepublik Deutschland
    Z Naturforsch C 42:1343-4. 1987
    ..1 degrees C. Our results show that "old" erythrocyte membrane proteins are less stable against thermal denaturation then "young" cells...
  43. ncbi Sequential regulation of ferroportin expression after erythrophagocytosis in murine macrophages: early mRNA induction by haem, followed by iron-dependent protein expression
    Constance Delaby
    INSERM U773, Centre de Recherche Biomedicale Bichat Beaujon CRB3, Universite Denis Diderot, site Bichat, 75870 Paris Cedex 18, France
    Biochem J 411:123-31. 2008
    ..These findings highlight a dual mechanism of Fpn regulation in BMDMs, characterized by early induction of the gene transcription predominantly mediated by haem, followed by iron-mediated post-transcriptional regulation of the exporter...
  44. doi Red cell transfusion in medicine: future challenges
    V M J Novotny
    Department of Blood Transfusion and Transplantation Immunology 469, Radboud University, Nijmegen Medical Centre, Nijmegen, The Netherlands
    Transfus Clin Biol 14:538-41. 2007
    ..It has been suggested that accelerated and/or aberrant forms of the physiological erythrocyte aging process underlie the red cell storage lesion...
  45. ncbi Spin label studies of erythrocytes during storage of blood
    G P Pessina
    Dipartimento di Chimica, Universita degli Studi di Siena, Italy
    Z Naturforsch C 44:824-8. 1989
    ..Signal decay of MAL-6 is a further proof that during storage of blood under blood bank conditions or during an artificial ageing of erythrocytes at 37 degrees C, profound modifications occur in the human erythrocyte membrane...
  46. ncbi Bovine red blood cell starvation age discrimination through a glutaraldehyde-amplified dielectrophoretic approach with buffer selection and membrane cross-linking
    Zachary Gagnon
    Department of Chemical and Biomolecular Engineering, Center for Microfluidics and Medical Diagnostics, University of Notre Dame, Notre Dame, IN 46556, USA
    Electrophoresis 29:2272-9. 2008
    ..treatment. It is then expected that rapid identification and sorting of healthy from diseased cells can be similarly sensitized...
  47. pmc Tank-tread frequency of the red cell membrane: dependence on the viscosity of the suspending medium
    Thomas M Fischer
    Institut fur Physiologie, Medizinische Fakultät der Rheinisch Westfälischen Technischen Hochschule Aachen, Aachen, Germany
    Biophys J 93:2553-61. 2007
    ..A fit of the data collected in this work to a theoretical red cell model might allow one to determine intrinsic mechanical constants in the low deformation regime...
  48. ncbi Erythrocyte aging and malaria
    I W Sherman
    Department of Biology, University of California, Riverside, CA 92521, USA
    Cell Mol Biol (Noisy-le-grand) 50:159-69. 2004
    ..Despite these parasite-induced age effects P. falciparum is able to avoid destruction by splenic removal through microvessel sequestration in the deep tissues via PS, clustered band 3 protein and adhesive neoantigens...
  49. pmc Effects of age-dependent membrane transport changes on the homeostasis of senescent human red blood cells
    Virgilio L Lew
    Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge, United Kingdom
    Blood 110:1334-42. 2007
    ..Based on these observations, a working hypothesis is proposed to explain the mechanism of progressive RBC densification with age and of the late reversal to a low-density condition with altered ionic gradients...
  50. ncbi Rescued mice with Hb E transgene-developed red cell changes similar to human beta-thalassemia/HbE disease
    Bundit Wannasuphaphol
    Thalassemia Research Center, Institute of Science and Technology for Research and Development, Mahidol University, Phuttamonthon, Nakornpathom 73170, Thailand
    Ann N Y Acad Sci 1054:407-16. 2005
    ..RBC life span and half-time of rescued red cells were shortened, indicating a rapid RBC destruction...
  51. ncbi Erythrocyte glycohydrolases in subjects with trisomy 21: could Down's syndrome be a model of accelerated ageing?
    L Massaccesi
    Department of Medical Chemistry, Biochemistry and Biotechnology, University of Milan, Via Saldini 50, 20133 Milan, Italy
    Mech Ageing Dev 127:324-31. 2006
    ..These findings indicate that erythrocyte ageing in DS differs partially from that of age-matched and elderly controls. In any case, the accelerated ageing seen in DS is no fully comparable to physiological ageing...
  52. ncbi Effect of chronic kidney disease on red blood cell rheology
    K Scott Brimble
    Department of Medicine, McMaster University, Hamilton, ON, Canada
    Clin Hemorheol Microcirc 34:411-20. 2006
    ..This study measured blood viscosity over a range of shear rates in erythropoietin-treated patients on hemodialysis (HD), and compared the findings to matched patients with chronic renal insufficiency (CRI) and healthy controls...
  53. ncbi Reduced expression of CD47 on stored red blood cells
    Rosemary Sparrow
    Transfusion 46:1263. 2006
  54. ncbi Cellular properties of human erythrocytes preserved in saline-adenine-glucose-mannitol in the presence of L-carnitine
    Arduino Arduini
    Department of Research and Development, Iperboreal Pharma S r l, Chieti, Italy
    Am J Hematol 82:31-40. 2007
    ..LC reduces the swelling of older cells during storage at 4 degrees C in SAG-M, possibly by acting on the permeability of cell membrane to monovalent cations...
  55. ncbi Stimulation of eryptosis by aluminium ions
    Olivier M Niemoeller
    Department of Physiology, University of Tubingen, Germany
    Toxicol Appl Pharmacol 217:168-75. 2006
    ..Both effects are expected to decrease the life span of circulating erythrocytes and presumably contribute to the development of anemia during Al(3+) intoxication...
  56. ncbi Clinical consequences of red cell storage in the critically ill
    Alan Tinmouth
    Center for Transfusion and Critical Care Research, Clinical Epidemiology Unit, Critical Care Program, University of Ottawa and Ottawa Health Research Institute, Ottawa, Ontario, Canada
    Transfusion 46:2014-27. 2006
    ..These data suggest a possible detrimental clinical effect associated with the transfusion of stored RBCs; randomized clinical trials further evaluating the clinical consequences of transfusing older stored RBCs are required...
  57. ncbi Characterization of red cell membrane proteins as a function of red cell density: annexin VII in different forms of hereditary spherocytosis
    Marianna Caterino
    Dipartimento di Biochimica e Biotecnologie Mediche, Universita di Napoli Federico II, Via Pansini, 5 80131 Napoli, Italy
    FEBS Lett 580:6527-32. 2006
    ..Cytoskeletal anomalies may then influence the turn-over of annexin VII during erythrocyte maturation...
  58. ncbi A fluorometric quantitative erythrophagocytosis assay using human THP-1 monocytic cells and PKH26-labelled red blood cells
    Geraldine Healey
    Research Unit, Australian Red Cross Blood Service, PO Box 354, South Melbourne, Victoria 3205 Australia
    J Immunol Methods 322:50-6. 2007
    ..The quantitative, fluorometric plate assay is very sensitive and has good reproducibility, making it a useful tool to investigate the biological mechanisms that regulate erythrophagocytosis of normal and diseased RBCs...
  59. ncbi Functional analysis of aquaporin-1 deficient red cells. The Colton-null phenotype
    J C Mathai
    Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205 2185, USA
    J Biol Chem 271:1309-13. 1996
    ..The ratio of AQP1-mediated Pf to Pd predicts the length of the aqueous pore to be 36 A...
  60. ncbi Iron homeostasis in chronic inflammation
    J Balla
    Department of Medicine, Division of Nephrology and Hemodialysis Unit, Medical and Health Science Center, University of Debrecen, Nagyerdei krt 98, H 4012 Debercen, Hungary
    Acta Physiol Hung 94:95-106. 2007
    ..The present review concerns the underlying alterations in iron metabolism induced by chronic inflammation that result in anemia...
  61. ncbi Identification of the molecular defect in the erythrocyte membrane skeleton of some kindreds with hereditary spherocytosis
    S R Goodman
    Blood 60:772-84. 1982
    ..It would appear therefore that the defective HS spectrin-protein-4.1 interaction in type I hereditary spherocytosis may be the primary molecular defect rather than a secondary phenomena...
  62. ncbi [Heme metabolism and oxidative stress]
    P A Kaliman
    Kharkiv Karazin National University
    Ukr Biokhim Zh 73:5-15. 2001
    ..transport and exchange processes of exogenous heme and heme liberated from erythrocyte hemoglobin under erythrocyte aging and hemolysis...
  63. ncbi The cytoskeleton in ATP-depleted erythrocytes: the effect of shape transformation
    Y Marikovsky
    Department of Membrane Research and Biophysics, Weizmann Institute of Science, Rehovot, Israel
    Mech Ageing Dev 86:137-44. 1996
    b>Erythrocyte aging, accompanied by reduced deformability and low ATP level, is considered to be among the factors limiting the survival of old erythrocytes...
  64. doi Oxidative stress due to aluminum exposure induces eryptosis which is prevented by erythropoietin
    Daiana M Vota
    Departamento de Quimica Biologica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellon II, Piso 4, Ciudad Universitaria, Ciudad Autonoma de Buenos Aires, C1428EHA, Argentina
    J Cell Biochem 113:1581-9. 2012
    ..Irrespective of the antioxidant mechanism, this property of EPO, shown in this model of Al exposure, let us suggest potential benefits by EPO treatment of patients with anemia associated to altered redox environment...
  65. pmc An RNAi therapeutic targeting Tmprss6 decreases iron overload in Hfe(-/-) mice and ameliorates anemia and iron overload in murine β-thalassemia intermedia
    Paul J Schmidt
    Department of Pathology, Children s Hospital Boston and Harvard Medical School, Boston, MA 02115, USA
    Blood 121:1200-8. 2013
    ....
  66. pmc Autoimmune haemolytic anaemias. IV. Biochemical studies of red cells from patients with autoimmune haemolytic anaemia with incomplete warm autoantibodies
    - Borne AE von dem
    Clin Exp Immunol 8:377-88. 1971
  67. doi Shortened lifespan of paroxysmal nocturnal haemoglobinuria-affected RBC estimated from differences in ratios of CD59-negative populations between reticulocytes and whole RBC
    H Ninomiya
    Institute of Clinical Medicine, University of Tsukuba, Tsukuba, Ibaraki, Japan
    Int J Lab Hematol 30:41-5. 2008
    ..The MLS, in individual patients, altered irrespective of RBC and percentage reticulocytes. The MLS calculated from our methods may be a parameter that evaluates the haemolytic conditions in PNH...
  68. ncbi Role of Se in stabilization of human erythrocyte membrane skeleton
    F Y Yang
    Institute of Biophysics, Academia Sinica, Beijing, China
    Biochem Int 15:475-82. 1987
    ..However, since GSHPx is mainly distributed in the cytoplasm of erythrocytes, the stabilizing effect of Se on erythrocyte membranes might not be related to the activity of this enzyme...
  69. ncbi Posttransfusion survival of red cells frozen for 8 weeks after 42-day liquid storage in AS-3
    E J Rathbun
    Department of Transfusion Medicine, Warren G Magnuson Clinical Center, Bethesda, Maryland
    Transfusion 29:213-7. 1989
    ..These findings show that an acceptable percentage of RBCs survives frozen storage after maximum liquid storage (mean ITE greater than 60%). If necessary, RBCs stored in AS-3 can be frozen at any time before 42 days...
  70. pmc Factor H mediated cell surface protection from complement is critical for the survival of PNH erythrocytes
    Viviana P Ferreira
    Department of Biochemistry, Center for Biomedical Research, University of Texas Health Science Center, Tyler, TX 75708, USA
    Blood 110:2190-2. 2007
    ..The results indicate that cells deficient in surface-bound regulators are protected for extended periods of time by factor H...
  71. ncbi Transgenic rescue of hemolytic anemia due to red blood cell pyruvate kinase deficiency
    Hitoshi Kanno
    Department of Transfusion Medicine and Cell Processing, Tokyo Women s Medical University, Tokyo, Japan
    Haematologica 92:731-7. 2007
    ..To test whether the targeted overexpression of the normal R-PK gene in erythroid cells could reduce hemolysis in R-PK mutant mice, we performed a genetic rescue study using human R-PK transgenic mice...
  72. ncbi Red cell adenylate kinase deficiency associated with hereditary nonspherocytic hemolytic anemia: clinical and biochemical studies
    S Miwa
    Am J Hematol 14:325-33. 1983
    ..The mode of inheritance of this case is obscure. The mechanism of hemolysis might be a structural gene mutation that caused altered electrophoretic and kinetic properties...
  73. ncbi The multiple functions of hemoglobin
    B Giardina
    Istituto di Chimica e Chimica Clinica, Facolta di Medicina e Chirurgia, Universita Cattolica, Roma, Italy
    Crit Rev Biochem Mol Biol 30:165-96. 1995
    ....
  74. ncbi Iron release, membrane protein oxidation and erythrocyte ageing
    C Signorini
    Institute of General Pathology, University of Siena, Italy
    FEBS Lett 362:165-70. 1995
    ..The addition of ferrozine during the aerobic incubation prevented both the IgG binding and the protein alterations seen in the IR spectra, suggesting an intracellular chelation of the released iron by ferrozine...
  75. ncbi Enhanced erythro-phagocytosis in polycythemic mice overexpressing erythropoietin
    Anna Bogdanova
    Institute of Veterinary Physiology, Vetsuisse Faculty and Zurich Center for Integrative Human Physiology ZIHP, University of Zurich, Switzerland
    Blood 110:762-9. 2007
    ..In conclusion, in tg6 mice erythrocyte aging is accelerated, which results, together with an increased number and activity of their macrophages, in ..
  76. ncbi Successful in vivo recovery and extended storage of additive solution (AS)-5 red blood cells after deglycerolization and resuspension in AS-3 for 15 days with an automated closed system
    Nicholas Bandarenko
    Transfusion Medicine Services, University of North Carolina Hospitals, Chapel Hill, North Carolina 27514, USA
    Transfusion 47:680-6. 2007
    ..Previously, cryopreserved red blood cell (RBC) units derived from CPD/AS-5 whole-blood (WB) collections have been limited to 24 hours postthaw storage (1-6 degrees C)...
  77. ncbi Force balance and membrane shedding at the red-blood-cell surface
    Pierre Sens
    Physico Chimie Théorique CNRS UMR 7083, ESPCI, 10 rue Vauquelin, 75231 Paris, France
    Phys Rev Lett 98:018102. 2007
    ..This model introduces the concept of force balance as a regulatory process at the cell membrane and quantitatively reproduces the rate of area loss in aging red-blood cells...
  78. ncbi Angiogenic endothelium shows lactadherin-dependent phagocytosis of aged erythrocytes and apoptotic cells
    Marcel H A M Fens
    Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, The Netherlands
    Blood 111:4542-50. 2008
    ..Implications of our findings for diagnostic and therapy of angiogenesis-driven diseases are discussed...
  79. ncbi Increase in Hb-O2-affinity at moderate altitude (2000 m) in patients on maintenance hemodialysis
    H Mairbaurl
    Institute of Physiology, University of Innsbruck, Austria
    Clin Nephrol 31:198-203. 1989
    ..We interpret the increase of the oxygen affinity of hemoglobin in patients with renal anemia as beneficial, as it favors oxygen loading of hemoglobin in the lung during exposure to a hypoxic environment...
  80. ncbi Significant calcium accumulation in fragile erythrocytes by dietary restriction in mice
    K Hishinuma
    Faculty of Agriculture, Tohoku University, Sendai, Nippon
    Int J Vitam Nutr Res 60:372-8. 1990
    ..of the erythrocytes, the oxygen supply to the peripheral tissues, may be maintained as 2,3-bisphosphoglyceric acid levels were not altered with erythrocyte aging in spite of the decreased glycolytic activity in the diet restricted mice.
  81. pmc Partitioning regulatory mechanisms of within-host malaria dynamics using the effective propagation number
    C J E Metcalf
    Department of Zoology, Oxford University, Oxford OX1 3PS, UK
    Science 333:984-8. 2011
    ..Such a statistical approach offers a tool to improve targeting of drugs or vaccines for human therapy by revealing the dynamics and interactions of within-host regulatory mechanisms...
  82. doi Chronic IFN-γ production in mice induces anemia by reducing erythrocyte life span and inhibiting erythropoiesis through an IRF-1/PU.1 axis
    Sten F Libregts
    Department of Experimental Immunology, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands
    Blood 118:2578-88. 2011
    ..Notably, down-regulation of either IRF-1 or PU.1 expression is sufficient to overcome IFN-γ-induced inhibition of erythropoiesis. These findings reveal a molecular mechanism by which chronic exposure to IFN-γ induces anemia...
  83. pmc Prestorage leukoreduction ameliorates the effects of aging on banked blood
    Herb A Phelan
    Division of Burns Trauma Critical Care, Department of Surgery, UT Southwestern Medical Center, Dallas, Texas 75390 9158, USA
    J Trauma 69:330-7. 2010
    ..The purpose of this retrospective cohort study was to describe the effect of prestorage leukoreduction (PS-LR) on the detrimental clinical effects of increasing age on blood products used in trauma patients...
  84. doi Increased cation conductance in human erythrocytes artificially aged by glycation
    Yuliya V Kucherenko
    Department of Physiology, Institute I, Eberhard Karls Universitat Tubingen, Gmelinstr 5, 72076, Tubingen, Germany
    J Membr Biol 235:177-89. 2010
    ..The present study explored whether glycation-induced erythrocyte aging is paralleled by features of suicidal erythrocyte death or eryptosis, which is characterized by cell ..
  85. ncbi Hemolysis and survival of autologous red blood cells salvaged after cemented and uncemented total hip arthroplasty
    Branko Tripkovic
    Orthopaedic Surgery Department, University Hospital Zagreb, Croatia
    Am J Orthop (Belle Mead NJ) 39:76-9. 2010
    ..Our study results confirmed that washing blood collected after surgery is not necessary. Not washing this blood is safe and decreases allogeneic transfusion in orthopedic procedures...
  86. pmc Hemolysis is associated with low reticulocyte production index and predicts blood transfusion in severe malarial anemia
    Rolf Fendel
    Medical Research Unit, Albert Schweitzer Hospital, Lambarene, Gabon
    PLoS ONE 5:e10038. 2010
    ..Emerging drug resistant parasites might aggravate the situation. This case control study investigates biomarkers of enhanced hemolysis in hospitalized children with either SMA or mild malaria (MM)...
  87. doi Fragmentation of human erythrocyte actin following exposure to hypoxia
    Angela Risso
    Departments of Biology and Plant Protection, M A T I Centre of Excellence, University of Udine, via delle Scienze 91, Udine, Italy
    Acta Haematol 123:6-13. 2010
    ..In conclusion, these results indicate that after exposure to hypoxia, RBCs display a modification of their actin and cytoskeleton instability...
  88. ncbi In vitro storage and in vivo survival studies of red cells from persons with the In(Lu) gene
    S K Ballas
    Cardeza Foundation for Hematologic Research, Department of Medicine, Thomas Jefferson University Hospital Blood Bank, Philadelphia, Pennsylvania
    Transfusion 32:607-11. 1992
    ..Individuals of the Lu(a-b-) phenotype, associated with In(Lu), may not be suitable candidates for routine blood donation...
  89. ncbi The effect of ammonium, phosphate, potassium, and hypotonicity on stored red cells
    A Kay
    Department of Molecular and Experimental Medicine, Scripps Clinic and Research Foundation, La Jolla, California
    Transfusion 32:37-41. 1992
    ..Despite prolonged ATP maintenance by ammonium and phosphate-containing storage media, satisfactory viability of RBCs stored up to 77 days was not demonstrated in the rabbit transfusion model...
  90. doi Erythrocyte ageing in vivo and in vitro: structural aspects and implications for transfusion
    G J C G M Bosman
    Department of Biochemistry, Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
    Transfus Med 18:335-47. 2008
    ....
  91. ncbi The measurement and importance of red cell survival
    Robert S Franco
    Division of Hematology Oncology, University of Cincinnati College of Medicine, Cincinnati, Ohio 45267 0508, USA
    Am J Hematol 84:109-14. 2009
    ..In this review, measurement techniques are placed in historical perspective and examined for relative merits and suitable application...
  92. doi Stycholysin II, a cytolysin from the sea anemone Stichodactyla helianthus promotes higher hemolysis in aged red blood cells
    Gloria Celedón
    Departamento de Fisiologia, Facultad de Ciencias, Universidad de Valparaiso, Chile
    Toxicon 51:1383-90. 2008
    ..It is concluded that this factor and the amount of intracellular K(+) are the dominant parameters that modulate the resistance of RBC to St II-induced lysis...
  93. pmc Simultaneous determination of cell aging and ATP release from erythrocytes and its implications in type 2 diabetes
    Wasanthi Subasinghe
    Department of Chemistry, Michigan State University, East Lansing, MI, USA
    Anal Chim Acta 618:227-33. 2008
    ..This determination provides evidence that antioxidant status in the RBC and its ability to release ATP, a known stimulus of nitric oxide production, are closely related...
  94. ncbi Adenosine protects against suicidal erythrocyte death
    Olivier M Niemoeller
    Department of Physiology, University of Tubingen, Tubingen, Germany
    Pflugers Arch 454:427-39. 2007
    ..d. at > or =10 microM). In conclusion, adenosine inhibits eryptosis by a mechanism presumably effective downstream of PKC. The effect may participate in the maintenance of microcirculation in ischemic tissue...
  95. ncbi Relationship between GHb concentration and erythrocyte survival determined from breath carbon monoxide concentration
    Mark A Virtue
    Minneapolis Veterans Administration Medical Center, Minneapolis, Minnesota 55417, USA
    Diabetes Care 27:931-5. 2004
    ..Subjects with decreased erythrocyte survival have an unusually low GHb percentage. The goal of this study was to determine whether hyperglycemia, as reflected by GHb percentage, is associated with decreased erythrocyte survival...
  96. ncbi SHPS-1 promotes the survival of circulating erythrocytes through inhibition of phagocytosis by splenic macrophages
    Tomomi Ishikawa-Sekigami
    Department of Medicine and Clinical Scinece, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan
    Blood 107:341-8. 2006
    ..These results suggest that SHPS-1 negatively regulates the phagocytosis of RBCs by splenic macrophages, thereby determining both the lifespan of individual RBCs and the number of circulating erythrocytes...
  97. ncbi In vivo studies of monoclonal anti-D and the mechanism of immune suppression
    Belinda M Kumpel
    International Blood Group Reference Laboratory, Bristol Institute of Transfusion Sciences, Southmead Road, Bristol BS10 5ND, UK
    Transfus Clin Biol 9:9-14. 2002
    ..The most likely mechanism of action was considered to be inhibition of B cells resulting from co-crosslinking antigen receptors with inhibitory Fc gamma R when the B cells contacted red cells that had bound passive anti-D...
  98. ncbi Iron release, oxidative stress and erythrocyte ageing
    Mario Comporti
    Department of Pathophysiology and Experimental Medicine, University of Siena, Siena, Italy
    Free Radic Biol Med 32:568-76. 2002
    ..In newborns the release of iron in erythrocytes is correlated with plasma nonprotein-bound iron and may contribute to its appearance...
  99. ncbi Different behavior of ghost-linked acidic and neutral sialidases during human erythrocyte ageing
    C Tringali
    Department of Medical Chemistry and Biochemistry, The Medical School, University of Milan, Italy
    Glycoconj J 18:407-18. 2001
    ..The enhanced neutral sialidase activity might account for the sialic acid loss occurring during erythrocyte ageing...
  100. ncbi Erythrocyte survival is promoted by plasma and suppressed by a Bak-derived BH3 peptide that interacts with membrane-associated Bcl-X(L)
    Melanie Walsh
    Department of Biochemistry and Biosciences Research Institute, National University of Ireland, Cork
    Blood 99:3439-48. 2002
    ..We conclude that the BH3 peptide accelerates erythrocyte death through antagonization of Bcl-X(L). The data suggest that erythrocyte survival is promoted by survival factors in plasma and by membrane-associated Bcl-X(L.)..
  101. ncbi Methionine oxidation and aging
    Earl R Stadtman
    Laboratory of Biochemistry, National Heart, Lung and Blood Institute, Bethesda, MD 20892, USA
    Biochim Biophys Acta 1703:135-40. 2005
    ..of proteins increases with age in a number of different aging models, including replicative senescence and erythrocyte aging, but not in mouse tissues during aging...

Research Grants82

  1. THE ROLE OF CONTRACTILE PROTEINS IN ERYTHROCYTE AGING
    DIANE LIN; Fiscal Year: 1980
    ..With these studies, we hope to establish a correlation between the state of the contractile proteins and the age-related rigidity of the red cell membrane...
  2. Defining the IsoAspartome
    Cheng Lin; Fiscal Year: 2010
    ..presence of isoAsp in a wide variety of systems from in vitro peptides to whole tumor cells to studies of erythrocyte aging. Finally, this study will apply these methods to detailed study of isoaspartic acid formation rates via both ..
  3. Defining the IsoAspartome
    Cheng Lin; Fiscal Year: 2009
    ..presence of isoAsp in a wide variety of systems from in vitro peptides to whole tumor cells to studies of erythrocyte aging. Finally, this study will apply these methods to detailed study of isoaspartic acid formation rates via both ..
  4. Defining the IsoAspartome
    PETER O CONNOR; Fiscal Year: 2007
    ..presence of isoAsp in a wide variety of systems from in vitro peptides to whole tumor cells to studies of erythrocyte aging. Finally, this study will apply these methods to detailed study of isoaspartic acid formation rates via both ..
  5. Reducing Mortality from Acute Hemorrhage in Trauma
    John Hess; Fiscal Year: 2006
    ..Reducing death from acute hemorrhage in trauma care will be a major step forward in reducing overall trauma mortality. ..
  6. Study of the HFE Transferrin Receptor interaction in mammalian iron homeostasis
    Paul Schmidt; Fiscal Year: 2008
    ..Most importantly, this award will assist in the transition of the candidate to become a fully independent investigator in the field of iron metabolism. ..
  7. Study of the HFE Transferrin Receptor interaction in mammalian iron homeostasis
    Paul Schmidt; Fiscal Year: 2007
    ..Most importantly, this award will assist in the transition of the candidate to become a fully independent investigator in the field of iron metabolism. [unreadable] [unreadable]..
  8. IMMUNOGLOBULIN V GENES AND B CELL DEVELOPMENT
    Richard Hardy; Fiscal Year: 2002
    ..The results of these studies will help to establish the types of selection that shape the expressed B cell repertoire, both fetally and in the adult, with implications for modulating normal and autoimmune responses. ..
  9. BD Biosciences LSR Flow Cytometer
    Richard Hardy; Fiscal Year: 2002
    ..abstract_text> ..
  10. IN UTERO STEM CELL TRANSFER IN MPS VII
    Jane Barker; Fiscal Year: 2001
    ....
  11. IMMUNOGLOBULIN V GENES AND B CELL DEVELOPMENT
    Richard Hardy; Fiscal Year: 2001
    ..The results of these studies will help to establish the types of selection that shape the expressed B cell repertoire, fetally and in the adult, with implications for modulating both normal and autoimmune responses. ..
  12. LY-1 B CELLS: DEVELOPMENTAL ORIGINS AND V GENE BIASES
    Richard Hardy; Fiscal Year: 2001
    ..abstract_text> ..
  13. LY-1 B CELLS--DEVELOPMENTAL ORIGINS AND V GENE
    Richard Hardy; Fiscal Year: 2000
    ..Cumulatively, these studies will provide a clearer picture of fetal and adult B lymphopoiesis and distinguish whether the modes of selection affecting developing B cells are similar or different during these two ontogenic timi s. ..
  14. HEMOLYTIC ANEMIAS--MODELS
    Jane Barker; Fiscal Year: 1992
    ....
  15. GENETIC ENGINEERING OF CELLS FROM ANEMIC ANIMALS
    Jane Barker; Fiscal Year: 2002
    ..abstract_text> ..
  16. IN UTERO STEM CELL TRANSFER IN MPS VII
    Jane Barker; Fiscal Year: 2002
    ....
  17. LY-1 B CELLS: DEVELOPMENTAL ORIGINS AND V GENE BIASES
    Richard Hardy; Fiscal Year: 2003
    ..abstract_text> ..
  18. LY-1 B CELLS: DEVELOPMENTAL ORIGINS AND V GENE BIASES
    Richard Hardy; Fiscal Year: 2009
    ....
  19. LY-1 B CELLS: DEVELOPMENTAL ORIGINS AND V GENE BIASES
    Richard Hardy; Fiscal Year: 2008
    ....
  20. LY-1 B CELLS: DEVELOPMENTAL ORIGINS AND V GENE BIASES
    Richard Hardy; Fiscal Year: 2007
    ....
  21. IMMUNOGLOBULIN V GENES AND B CELL DEVELOPMENT
    Richard Hardy; Fiscal Year: 2006
    ..The results of these studies will help to establish the types of selection that shape the expressed B cell repertoire, both fetally and in the adult, with implications for modulating normal and autoimmune responses. ..
  22. IMMUNOGLOBULIN V GENES AND B CELL DEVELOPMENT
    Richard Hardy; Fiscal Year: 2005
    ..The results of these studies will help to establish the types of selection that shape the expressed B cell repertoire, both fetally and in the adult, with implications for modulating normal and autoimmune responses. ..
  23. LY-1 B CELLS: DEVELOPMENTAL ORIGINS AND V GENE BIASES
    Richard Hardy; Fiscal Year: 2005
    ..abstract_text> ..
  24. Hemolytic Anemia: Models
    Jane Barker; Fiscal Year: 2004
    ..5 isoform in the structure and assembly of the sarcoplasmic reticulum by an in vivo developmental study. ..
  25. IN UTERO STEM CELL TRANSFER IN MPS VII
    Jane Barker; Fiscal Year: 2004
    ..abstract_text> ..
  26. IMMUNOGLOBULIN V GENES AND B CELL DEVELOPMENT
    Richard Hardy; Fiscal Year: 2004
    ..The results of these studies will help to establish the types of selection that shape the expressed B cell repertoire, both fetally and in the adult, with implications for modulating normal and autoimmune responses. ..
  27. IN UTERO STEM CELL TRANSFER IN MPS VII
    Jane Barker; Fiscal Year: 2003
    ..abstract_text> ..
  28. IMMUNOGLOBULIN V GENES AND B CELL DEVELOPMENT
    Richard Hardy; Fiscal Year: 2003
    ..The results of these studies will help to establish the types of selection that shape the expressed B cell repertoire, both fetally and in the adult, with implications for modulating normal and autoimmune responses. ..
  29. GENETIC ENGINEERING OF CELLS FROM ANEMIC ANIMALS
    Jane Barker; Fiscal Year: 2003
    ..abstract_text> ..
  30. HEMOLYTIC ANEMIAS--MODELS
    Jane Barker; Fiscal Year: 2002
    ..abstract_text> ..
  31. IMMUNOGLOBULIN V GENES AND B CELL DEVELOPMENT
    Richard Hardy; Fiscal Year: 1999
    ..The results of these studies will help to establish the types of selection that shape the expressed B cell repertoire, fetally and in the adult, with implications for modulating both normal and autoimmune responses. ..
  32. GENETIC ENGINEERING OF CELLS FROM ANEMIC ANIMALS
    Jane Barker; Fiscal Year: 1999
    ..abstract_text> ..
  33. IN UTERO STEM CELL TRANSFER IN MPS VII
    Jane Barker; Fiscal Year: 1999
    ....
  34. LY-1 B CELLS--DEVELOPMENTAL ORIGINS AND V GENE
    Richard Hardy; Fiscal Year: 1999
    ..Cumulatively, these studies will provide a clearer picture of fetal and adult B lymphopoiesis and distinguish whether the modes of selection affecting developing B cells are similar or different during these two ontogenic timi s. ..
  35. GENETIC ENGINEERING OF CELLS FROM ANEMIC ANIMALS
    Jane Barker; Fiscal Year: 1990
    ..W41/W39 blastocysts will be used because their cells will not compete with cells from the alpha thalassemic teratocarcinomas or from the "cured" W41/W39 teratocarcinomas to populate the erythrypoietic organs...
  36. HEMOLYTIC ANEMIAS--MODELS
    Jane Barker; Fiscal Year: 1993
    ....
  37. HEMOLYTIC ANEMIAS MODELS
    Jane Barker; Fiscal Year: 1990
    ....
  38. GENETIC ENGINEERING OF CELLS FROM ANEMIC ANIMALS
    Jane Barker; Fiscal Year: 1992
    ..W41/W39 blastocysts will be used because their cells will not compete with cells from the alpha thalassemic teratocarcinomas or from the "cured" W41/W39 teratocarcinomas to populate the erythrypoietic organs...
  39. Pulmonary Hypertension in Thalassemia
    SYLVIA SINGER; Fiscal Year: 2008
    ....
  40. Pulmonary Hypertension in Thalassemia
    SYLVIA SINGER; Fiscal Year: 2007
    ....
  41. HEMOLYTIC ANEMIAS--MODELS
    Jane Barker; Fiscal Year: 2000
    ..abstract_text> ..
  42. GENETIC ENGINEERING OF CELLS FROM ANEMIC ANIMALS
    Jane Barker; Fiscal Year: 2000
    ..abstract_text> ..
  43. LY-1 B CELLS--LINEAGE DETERMINATION/ANTIBODY REPERTOIRE
    Richard Hardy; Fiscal Year: 1992
    ..Specificities encoded by these V genes to be examined in detail will include both anti-thymocyte and anti-bromelain-treated mouse erythrocyte autoantibodies, both distinctively enriched in this population...
  44. LY-1 B CELLS: DEVELOPMENTAL ORIGINS AND V GENE BIASES
    Richard Hardy; Fiscal Year: 2002
    ..abstract_text> ..
  45. IN UTERO STEM CELL TRANSFER IN MPS VII
    Jane Barker; Fiscal Year: 2000
    ....
  46. HEMOLYTIC ANEMIAS--MODELS
    Jane Barker; Fiscal Year: 2001
    ..abstract_text> ..
  47. HEMOLYTIC ANEMIAS MODELS
    Jane Barker; Fiscal Year: 1991
    ....
  48. IMMUNOGLOBULIN V GENES AND B CELL DEVELOPMENT
    Richard Hardy; Fiscal Year: 2000
    ..The results of these studies will help to establish the types of selection that shape the expressed B cell repertoire, fetally and in the adult, with implications for modulating both normal and autoimmune responses. ..
  49. FACSVantage SE Sorting Flow Cytometer
    Jane Barker; Fiscal Year: 2002
    ..Funds are requested to purchase the FACSVantage SE and the optional equipment for TJL's Flow Cytometry Service. This will provide a cost-effective way to fill an urgent need of TJL's principal investigators. ..
  50. LY-1 B CELLS--LINEAGE DETERMINATION/ANTIBODY REPERTOIRE
    Richard Hardy; Fiscal Year: 1991
    ..Specificities encoded by these V genes to be examined in detail will include both anti-thymocyte and anti-bromelain-treated mouse erythrocyte autoantibodies, both distinctively enriched in this population...
  51. Hemolytic Anemia: Models
    Jane Barker; Fiscal Year: 2003
    ..5 isoform in the structure and assembly of the sarcoplasmic reticulum by an in vivo developmental study. ..
  52. LY-1 B CELLS: DEVELOPMENTAL ORIGINS AND V GENE BIASES
    Richard Hardy; Fiscal Year: 2004
    ..abstract_text> ..
  53. HEMOLYTIC ANEMIAS--MODELS
    Jane Barker; Fiscal Year: 1999
    ..abstract_text> ..