Research Topics
Genomes and Genes
| erythrocyte agingSummarySummary: The senescence of RED BLOOD CELLS. Lacking the organelles that make protein synthesis possible, the mature erythrocyte is incapable of self-repair, reproduction, and carrying out certain functions performed by other cells. This limits the average life span of an erythrocyte to 120 days. Top Publications
Research Grants
| Scientific Experts
|
Detail Information
Publications
Storage-related changes in erythrocyte band 3: not a case for the Diego blood group antigensG J C G M Bosman
Department of Biochemistry, University Medical Centre Nijmegen, NL 6500 HB Nijmegen, The Netherlands
Cell Mol Biol (Noisy-le-grand) 51:195-200. 2005..This makes it unlikely that immunological removal of erythrocytes after transfusion is mediated by reactions involving the Diego blood group system...
Quantitative analysis of mechanisms that govern red blood cell age structure and dynamics during anaemiaNicholas J Savill
Centre for Infectious Diseases, Institute of Immunology and Infection Research, University of Edinburgh, Edinburgh, United Kingdom
PLoS Comput Biol 5:e1000416. 2009..We also provide evidence of large diurnal oscillations in serum erythropoietin levels during anaemia...- Acidic and neutral sialidase in the erythrocyte membrane of type 2 diabetic patientsBruno Venerando
Department of Medical Chemistry and Biochemistry, School of Medicine, University of Milan, LITA, Via Fratelli Cervi, 93 20090 Segrate, Milan, Italy
Blood 99:1064-70. 2002..It has been suggested that the expression of the neutral enzyme requires some activation mechanism that is impaired in diabetes... 
Comparative proteomics of erythrocyte aging in vivo and in vitroG J C G M Bosman
Department of Biochemistry, Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen Medical Centre, The Netherlands
J Proteomics 73:396-402. 2010..maintain erythrocyte homeostasis; (2) open new roads to elucidate the processes that regulate physiological erythrocyte aging and removal, and thereby; (3) provide the foundation for rational interventions to prevent untimely ..- Senescent erythrocytes: modification of rheologic properties, antigenic expression and interaction with monocytesA Racca
Laboratorio Inmunohematología, Hemorreología e Inmunogenética, Facultad de Ciencias Bioquimicas y Farmaceuticas, Universidad Nacional de Rosario, Argentina
Medicina (B Aires) 59:33-7. 1999..01). The significant modifications in the biomechanic properties of senescent red blood cell membrane and the loss of antigenic expression could lead to physiological phagocytosis... - Band 3/complement-mediated recognition and removal of normally senescent and pathological human erythrocytesPaolo Arese
Department of Genetics, Biology and Biochemistry, University of Torino Medical School, Torino
Cell Physiol Biochem 16:133-46. 2005..falciparum malaria, may lead to massive and rapid destruction of RBCs by a mechanism comparable to a dramatic, time-compressed enhancement of normal RBC senescence... - Erythrocyte aging in neurodegenerative disordersL de Franceschi
Department of Clinical and Experimental Medicine, Section of Internal Medicine, University of Verona, Policlinico GB Rossi, P le L Scuro 10, 37134 Verona, Italy
Cell Mol Biol (Noisy-le-grand) 50:179-85. 2004..We have reviewed the mechanism(s) of the loss of red cell membrane stability and of the precocious red cell aging in neurodegenerative disorders... - A detailed study of time-dependent changes in human red blood cells: from reticulocyte maturation to erythrocyte senescenceSean C Gifford
Visual and Circulatory Biophysics Laboratory, Department of Biomedical Engineering, Boston University, Boston, MA, USA
Br J Haematol 135:395-404. 2006..15%) and a model describing the complete time-course of diminishing mean RBC area and Hb is proposed. The relationship between the losses of Hb and area, and their possible influence on red cell lifespan, are discussed... - Erythrocyte aging: a more than superficial resemblance to apoptosis?Giel J C G M Bosman
Department of Biochemistry, Radboud University Nijmegen Medical Center, Nijmegen Center for Molecular Life Sciences, Nijmegen, The Netherlands
Cell Physiol Biochem 16:1-8. 2005In physiological circumstances, erythrocyte aging leads to binding of autologous IgG followed by recognition and removal through phagocytosis, mainly by Kupffer cells in the liver... - Characterization of red cell membrane proteins as a function of red cell density: annexin VII in different forms of hereditary spherocytosisMarianna Caterino
Dipartimento di Biochimica e Biotecnologie Mediche, Universita di Napoli Federico II, Via Pansini, 5 80131 Napoli, Italy
FEBS Lett 580:6527-32. 2006..Cytoskeletal anomalies may then influence the turn-over of annexin VII during erythrocyte maturation... - Time course of the age-related alterations in stored bloodSibel Türkes
Physics Engineering Department, Faculty of Engineering, Hacettepe University, Beytepe, Ankara 06532, Turkey
Biophys Chem 105:143-50. 2003..Two exponential curves attributed to the liquid and cellular parts of a given samples were found to be described best in the reduction of MAL-6 spin label in WB, AE and AAE... - Accelerated senescence of human erythrocytes cultured with Plasmodium falciparumFausta Omodeo-Salè
Istituto di Fisiologia Generale e Chimica Biologica Giovanni Esposito, Facolta di Farmacia, Via Trentacoste 2, 20134 Milano, Italy
Blood 102:705-11. 2003..In summary, we demonstrated that membrane damage leading to accelerated senescence of both infected and uninfected erythrocytes will likely contribute to malaria anemia... - Oxygen transport in blood at high altitude: role of the hemoglobin-oxygen affinity and impact of the phenomena related to hemoglobin allosterism and red cell functionMichele Samaja
Dipartimento di Medicina, Chirurgia e Odontoiatria, Ospedale San Paolo, Universita di Milano, Via di Rudini 8, 20142 Milan, Italy
Eur J Appl Physiol 90:351-9. 2003.... - Clinical consequences of red cell storage in the critically illAlan Tinmouth
Center for Transfusion and Critical Care Research, Clinical Epidemiology Unit, Critical Care Program, University of Ottawa and Ottawa Health Research Institute, Ottawa, Ontario, Canada
Transfusion 46:2014-27. 2006..These data suggest a possible detrimental clinical effect associated with the transfusion of stored RBCs; randomized clinical trials further evaluating the clinical consequences of transfusing older stored RBCs are required... - Splenectomy prolongs in vivo survival of erythrocytes differently in spectrin/ankyrin- and band 3-deficient hereditary spherocytosisRamune Reliene
Institute of Biochemistry, Swiss Federal Institute of Technology, , CH 8093 Zurich, Switzerland
Blood 100:2208-15. 2002.... - Increased release of free Fe ions in human erythrocytes during aging in the circulationKen Ando
School of Pharmacy, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan
Free Radic Res 36:1079-84. 2002..The results indicated that free Fe ions were released from erythrocytes during aging process in the circulation. Released free Fe ions would promote oxidative damages of the cells during aging process... - A fluorometric quantitative erythrophagocytosis assay using human THP-1 monocytic cells and PKH26-labelled red blood cellsGeraldine Healey
Research Unit, Australian Red Cross Blood Service, PO Box 354, South Melbourne, Victoria 3205 Australia
J Immunol Methods 322:50-6. 2007..The quantitative, fluorometric plate assay is very sensitive and has good reproducibility, making it a useful tool to investigate the biological mechanisms that regulate erythrophagocytosis of normal and diseased RBCs... - Hydrogen-peroxide-induced heme degradation in red blood cells: the protective roles of catalase and glutathione peroxidaseEnika Nagababu
Molecular Dynamics Section, Gerontology Research Center, National Institute on Aging, National Institutes of Health, 5600 Nathan Shock Drive, Baltimore, MD 21224, USA
Biochim Biophys Acta 1620:211-7. 2003..The unique role of GSHPX is attributed to its ability to react with hydrogen peroxide generated in close proximity to the red cell membrane in conjunction with the autoxidation of membrane-bound hemoglobin... - Fas-, caspase 8-, and caspase 3-dependent signaling regulates the activity of the aminophospholipid translocase and phosphatidylserine externalization in human erythrocytesDebabrata Mandal
Department of Chemistry, Bose Institute 93 1 Acharya Prafulla Chandra Road, Kolkata 700009, India
J Biol Chem 280:39460-7. 2005..Our data demonstrate for the first time the novel involvement of Fas/caspase 8/caspase 3-dependent signaling in an enucleated cell leading to PS externalization, a central feature of erythrophagocytosis and erythrocyte biology... - Phosphatidylserine externalization in sickle red blood cells: associations with cell age, density, and hemoglobin FZahida Yasin
Vontz Center for Molecular Studies, Hematology Oncology Division ML 0508, Department of Internal Medicine, University of Cincinnati College of Medicine, 3125 Eden Avenue, Cincinnati, OH, USA
Blood 102:365-70. 2003..These studies indicate that PS externalization in sickle cells may be low level, as observed in many immature cells, or high level, which is associated with dehydration and appears to be more specific for sickle RBCs... - Molecular and cellular mechanisms of erythrocyte programmed cell death: impact on blood transfusionD Bratosin
National Institute of Biological Science Research and Development, Bucarest, Rumania
Vox Sang 83:307-10. 2002 - Characterization of binding and phagocytosis of oxidatively damaged erythrocyte to macrophageHong Sook Ko
Division of Cardiology, Department of Internal Medicine, College of Medicine, Chung-Ang University, Seoul, Korea
Korean J Intern Med 17:220-6. 2002..CONCLUSION: OxRBCs might bind and be taken up to macrophages not mainly through class A nor B scavenger receptors, but through other scavenger receptors and/or pathways. These processes are dynamic and ionic strength might be involved... - Mapping of senescent cell antigen on brain anion exchanger protein (AE) isoforms using HPLC and fast atom bombardment ionization mass spectrometry (FAB-MS)Marguerite M Kay
International Foundation for Biomedical Aging Research, PO Box 1887, Temple, TX 76503 1887, USA
J Mol Recognit 17:33-40. 2004..This provides confirmation that SCA is on non-erythroid cell types. Implications of these studies to the generation of neoantigens by oxidation and their recognition by autoantibodies to them are discussed... - Phosphotyrosine phosphatases acting on band 3 in human erythrocytes of different age: PTP1B processing during cell ageingAnnarita Ciana
Universita di Pavia, Dipartimento di Biochimica A Castellani, Sezione di Scienze, via Bassi, 21, 27100 Pavia, Italy
Bioelectrochemistry 62:169-73. 2004.... - Experiences in the measurement of RBC-bound IgG as markers of cell ageRenata Paleari
Dipartimento di Scienze e Tecnologie Biomediche, , Via Fratelli Cervi 93, 20090 Segrate, Milan, Italy
Bioelectrochemistry 62:175-9. 2004..A significantly greater number of RBC-bound IgG was measured in splenectomized beta-thalassemia intermedia patients and in subjects with secondary decreases in the C3 complement fraction concentration... - Rescued mice with Hb E transgene-developed red cell changes similar to human beta-thalassemia/HbE diseaseBundit Wannasuphaphol
Thalassemia Research Center, Institute of Science and Technology for Research and Development, Mahidol University, Phuttamonthon, Nakornpathom 73170, Thailand
Ann N Y Acad Sci 1054:407-16. 2005..RBC life span and half-time of rescued red cells were shortened, indicating a rapid RBC destruction... - Erythrocyte glycohydrolases in subjects with trisomy 21: could Down's syndrome be a model of accelerated ageing?L Massaccesi
Department of Medical Chemistry, Biochemistry and Biotechnology, University of Milan, Via Saldini 50, 20133 Milan, Italy
Mech Ageing Dev 127:324-31. 2006..These findings indicate that erythrocyte ageing in DS differs partially from that of age-matched and elderly controls. In any case, the accelerated ageing seen in DS is no fully comparable to physiological ageing... - Interlaboratory comparison of red-cell ATP, 2,3-diphosphoglycerate and haemolysis measurementsJ R Hess
University of Maryland Medical School, Baltimore, MD 21201, USA
Vox Sang 89:44-8. 2005..The reproducibility of RBC ATP, DPG and haemolysis measurements between centres was investigated... - [Erythrophagocytosis and recycling of heme iron in normal and pathological conditions; regulation by hepcidin]C Beaumont
Inserm U656, Faculte Xavier Bichat, Paris
Transfus Clin Biol 12:123-30. 2005..Perturbations in erythrophagocytosis play a physiopathological role in several diseases, including hemochromatosis, anemia of chronic disorders and thalassemia... - The 24-hour posttransfusion survival of baboon red blood cells preserved in citrate phosphate dextrose/ ADSOL (CPD/AS-1) for 49 daysC Robert Valeri
Naval Blood Research Laboratory, Boston University School of Medicine, 615 Albany Street, Boston, Massachusetts 02118, USA
Contemp Top Lab Anim Sci 44:38-40. 2005..Further storage for 42 and 49 days resulted in lower values for baboon RBCs compared with human RBCs... - Effect of chronic kidney disease on red blood cell rheologyK Scott Brimble
Department of Medicine, McMaster University, Hamilton, ON, Canada
Clin Hemorheol Microcirc 34:411-20. 2006..This study measured blood viscosity over a range of shear rates in erythropoietin-treated patients on hemodialysis (HD), and compared the findings to matched patients with chronic renal insufficiency (CRI) and healthy controls... - Reduced expression of CD47 on stored red blood cellsRosemary Sparrow
Transfusion 46:1263. 2006 - Cellular properties of human erythrocytes preserved in saline-adenine-glucose-mannitol in the presence of L-carnitineArduino Arduini
Department of Research and Development, Iperboreal Pharma S r l, Chieti, Italy
Am J Hematol 82:31-40. 2007..LC reduces the swelling of older cells during storage at 4 degrees C in SAG-M, possibly by acting on the permeability of cell membrane to monovalent cations... - The in vitro quality of washed, prestorage leucocyte-depleted red blood cell concentratesV Weisbach
Department of Transfusion Medicine and Haemostaseology, Friedrich Alexander Universitat Erlangen Nurnberg, Erlangen, Germany
Vox Sang 87:19-26. 2004..No data are currently available on the quality of washed prestorage leucocyte-depleted red blood cell concentrates (RCCs)... - Survival of the fittest?--survival of stored red blood cells after transfusionM Luten
Department of Research and Development, Sanquin Blood Bank Southeast Region, P O Box 1013, 6501 BA Nijmegen, The Netherlands
Cell Mol Biol (Noisy-le-grand) 50:197-203. 2004..1% for the measurement of survival in vivo based on antigenic differences. This technique has various advantages compared with the 'classical' 51Cr survival method... - Stimulation of eryptosis by aluminium ionsOlivier M Niemoeller
Department of Physiology, , Germany
Toxicol Appl Pharmacol 217:168-75. 2006..Both effects are expected to decrease the life span of circulating erythrocytes and presumably contribute to the development of anemia during Al(3+) intoxication... - The red cell revisited--matters of life and deathJ M Werre
Department of Blood Transfusion and Transplantation Immunology, University Medical Center, Nijmegen, The Netherlands
Cell Mol Biol (Noisy-le-grand) 50:139-45. 2004..Removal of old erythrocytes from the circulation and from donor blood may open new ways into the treatment of both bilirubin and secondary iron overload... - Band 3 and its alterations in health and diseaseM M Kay
Department of Neurology, University of Geneva Hospital, Micheli du Crest 24, CH 1211, Geneva, Switzerland
Cell Mol Biol (Noisy-le-grand) 50:117-38. 2004..Mutations in other genes can cause changes in band 3. An example is sickle cell anemia where the increased oxidation causes accelerated aging of band 3 and increased IgG binding and cellular removal... - Iron homeostasis in chronic inflammationJ Balla
Department of Medicine, Division of Nephrology and Hemodialysis Unit, Medical and Health Science Center, University of Debrecen, Nagyerdei krt 98, H 4012 Debercen, Hungary
Acta Physiol Hung 94:95-106. 2007..The present review concerns the underlying alterations in iron metabolism induced by chronic inflammation that result in anemia... - Effects of age-dependent membrane transport changes on the homeostasis of senescent human red blood cellsVirgilio L Lew
Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge, United Kingdom
Blood 110:1334-42. 2007..Based on these observations, a working hypothesis is proposed to explain the mechanism of progressive RBC densification with age and of the late reversal to a low-density condition with altered ionic gradients... - Sequential regulation of ferroportin expression after erythrophagocytosis in murine macrophages: early mRNA induction by haem, followed by iron-dependent protein expressionConstance Delaby
INSERM U773, Centre de Recherche Biomedicale Bichat Beaujon CRB3, Universite Denis Diderot, site Bichat, 75870 Paris Cedex 18, France
Biochem J 411:123-31. 2008..These findings highlight a dual mechanism of Fpn regulation in BMDMs, characterized by early induction of the gene transcription predominantly mediated by haem, followed by iron-mediated post-transcriptional regulation of the exporter... - Red cell transfusion in medicine: future challengesV M J Novotny
Department of Blood Transfusion and Transplantation Immunology 469, Radboud University, Nijmegen Medical Centre, Nijmegen, The Netherlands
Transfus Clin Biol 14:538-41. 2007..It has been suggested that accelerated and/or aberrant forms of the physiological erythrocyte aging process underlie the red cell storage lesion... - Identification of the molecular defect in the erythrocyte membrane skeleton of some kindreds with hereditary spherocytosisS R Goodman
Blood 60:772-84. 1982..It would appear therefore that the defective HS spectrin-protein-4.1 interaction in type I hereditary spherocytosis may be the primary molecular defect rather than a secondary phenomena... - Functional analysis of aquaporin-1 deficient red cells. The Colton-null phenotypeJ C Mathai
Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205 2185, USA
J Biol Chem 271:1309-13. 1996..The ratio of AQP1-mediated Pf to Pd predicts the length of the aqueous pore to be 36 A... - Bovine red blood cell starvation age discrimination through a glutaraldehyde-amplified dielectrophoretic approach with buffer selection and membrane cross-linkingZachary Gagnon
Department of Chemical and Biomolecular Engineering, Center for Microfluidics and Medical Diagnostics, University of Notre Dame, Notre Dame, IN 46556, USA
Electrophoresis 29:2272-9. 2008..treatment. It is then expected that rapid identification and sorting of healthy from diseased cells can be similarly sensitized... - Erythrocyte aging and malariaI W Sherman
Department of Biology, University of California, Riverside, CA 92521, USA
Cell Mol Biol (Noisy-le-grand) 50:159-69. 2004..Despite these parasite-induced age effects P. falciparum is able to avoid destruction by splenic removal through microvessel sequestration in the deep tissues via PS, clustered band 3 protein and adhesive neoantigens... - A genetic defect in the binding of protein 4.1 to spectrin in a kindred with hereditary spherocytosisL C Wolfe
N Engl J Med 307:1367-74. 1982..1, but the other functioned normally. Presumable, the nonfunctional spectrin was the product of the autosomal dominant gene responsible for the hereditary spherocytosis in this kindred... - Characterization of the calpastatin defect in erythrocytes from patients with essential hypertensionS Pontremoli
Institute of Biological Chemistry, University of Genoa, Italy
Biochem Biophys Res Commun 157:867-74. 1988.... - Thermal lability of membrane proteins of age separated erythrocytes as studied by electron spin resonance spin label techniqueG Bartosz
Experimentalphysik III, Gesamthochschule Kassel, Bundesrepublik Deutschland
Z Naturforsch C 42:1343-4. 1987..1 degrees C. Our results show that "old" erythrocyte membrane proteins are less stable against thermal denaturation then "young" cells... - Spin label studies of erythrocytes during storage of bloodG P Pessina
Dipartimento di Chimica, Universita degli Studi di Siena, Italy
Z Naturforsch C 44:824-8. 1989..Signal decay of MAL-6 is a further proof that during storage of blood under blood bank conditions or during an artificial ageing of erythrocytes at 37 degrees C, profound modifications occur in the human erythrocyte membrane... - Rapid cell corpse clearance by stabilin-2, a membrane phosphatidylserine receptorS Y Park
Department of Biochemistry and Cell Biology, Cell and Matrix Research Institute, School of Medicine, Kyungpook National University, Daegu, Korea
Cell Death Differ 15:192-201. 2008..These data indicate that stabilin-2 is the first of the membrane PS receptors to provide tethering and tickling signals, and may also be involved in the resolution of inflammation and the prevention of autoimmunity... - Effects of physiological parameters on the electrical properties of blood bank stored erythrocyte suspensionsMana Sezdi
Bogazici University, Institute of Biomedical Engineering, Istanbul
Conf Proc IEEE Eng Med Biol Soc 1:5133-6. 2006..By applying the multi-regression analysis, it is concluded that Ri, Re and Cm are appropriate for modeling Na+, K+, Cl-, pH and ATP during blood bank storage and predicting blood quality... - Albumin-quercetin combination offers a therapeutic advantage in the prevention of reduced survival of erythrocytes in visceral leishmaniasisGargi Sen
Indian Association for Cultivation of Science, Kolkata 700032, India
Blood Cells Mol Dis 39:245-54. 2007..Thus, we speculate that suppression of iron decompartmentalization, with the combination of quercetin and serum albumin might be a new approach in the prevention of reduced survival of erythrocytes in visceral leishmaniasis... - Tank-tread frequency of the red cell membrane: dependence on the viscosity of the suspending mediumThomas M Fischer
Institut fur Physiologie, Medizinische Fakultät der Rheinisch Westfälischen Technischen Hochschule Aachen, Aachen, Germany
Biophys J 93:2553-61. 2007..A fit of the data collected in this work to a theoretical red cell model might allow one to determine intrinsic mechanical constants in the low deformation regime... - Human erythroid cells are affected by aluminium. Alteration of membrane band 3 proteinDaniela Vittori
Laboratorio de Analisis Biologicos, Departamento de Quimica Biologica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellon II, Piso 4 Ciudad Universitaria, 1428 Buenos Aires, Argentina
Biochim Biophys Acta 1558:142-50. 2002..The present in vitro results suggest that Al may disturb human erythropoiesis through combined effects on mature erythrocytes and cellular metabolism in late erythroid progenitors... - Production of pathogen-inactivated RBC concentrates using PEN110 chemistry: a Phase I clinical studyJames P AuBuchon
Cell Labeling Laboratory, Department of Pathology, Dartmouth-Hitchcock Medical Center, Lebanon, New Hampshire, USA
Transfusion 42:146-52. 2002..Hemolysis remained below 1 percent; supernatant potassium and ATP levels were lower than those seen in control RBCs. CONCLUSION: PEN110-treated RBCs stored for 28 days meet all critical requirements for therapeutically useful units... - Storage-dependent remodeling of the red blood cell membrane is associated with increased immunoglobulin G binding, lipid raft rearrangement, and caspase activationAnastasios G Kriebardis
Department of Cell Biology and Biophysics, Faculty of Biology, University of Athens, Panepistimiopolis, Greece
Transfusion 47:1212-20. 2007..The membrane hosts critical steps in these mechanisms and undergoes widespread remodeling over the storage period... - [Heme metabolism and oxidative stress]P A Kaliman
Kharkiv Karazin National University
Ukr Biokhim Zh 73:5-15. 2001..transport and exchange processes of exogenous heme and heme liberated from erythrocyte hemoglobin under erythrocyte aging and hemolysis... - The cytoskeleton in ATP-depleted erythrocytes: the effect of shape transformationY Marikovsky
Department of Membrane Research and Biophysics, Weizmann Institute of Science, Rehovot, Israel
Mech Ageing Dev 86:137-44. 1996b>Erythrocyte aging, accompanied by reduced deformability and low ATP level, is considered to be among the factors limiting the survival of old erythrocytes... - Prestorage leukoreduction ameliorates the effects of aging on banked bloodHerb A Phelan
Division of Burns Trauma Critical Care, Department of Surgery, UT Southwestern Medical Center, Dallas, Texas 75390 9158, USA
J Trauma 69:330-7. 2010..The purpose of this retrospective cohort study was to describe the effect of prestorage leukoreduction (PS-LR) on the detrimental clinical effects of increasing age on blood products used in trauma patients... - Successful in vivo recovery and extended storage of additive solution (AS)-5 red blood cells after deglycerolization and resuspension in AS-3 for 15 days with an automated closed systemNicholas Bandarenko
Transfusion Medicine Services, University of North Carolina Hospitals, Chapel Hill, North Carolina 27514, USA
Transfusion 47:680-6. 2007..Previously, cryopreserved red blood cell (RBC) units derived from CPD/AS-5 whole-blood (WB) collections have been limited to 24 hours postthaw storage (1-6 degrees C)... - Hemolysis is associated with low reticulocyte production index and predicts blood transfusion in severe malarial anemiaRolf Fendel
Medical Research Unit, Albert Schweitzer Hospital, Lambarene, Gabon
PLoS ONE 5:e10038. 2010..Emerging drug resistant parasites might aggravate the situation. This case control study investigates biomarkers of enhanced hemolysis in hospitalized children with either SMA or mild malaria (MM)... - Hemolysis and survival of autologous red blood cells salvaged after cemented and uncemented total hip arthroplastyBranko Tripkovic
Orthopaedic Surgery Department, University Hospital Zagreb, Croatia
Am J Orthop (Belle Mead NJ) 39:76-9. 2010..Our study results confirmed that washing blood collected after surgery is not necessary. Not washing this blood is safe and decreases allogeneic transfusion in orthopedic procedures... - Partitioning regulatory mechanisms of within-host malaria dynamics using the effective propagation numberC J E Metcalf
Department of Zoology, Oxford University, Oxford OX1 3PS, UK
Science 333:984-8. 2011..Such a statistical approach offers a tool to improve targeting of drugs or vaccines for human therapy by revealing the dynamics and interactions of within-host regulatory mechanisms... - Chronic IFN-γ production in mice induces anemia by reducing erythrocyte life span and inhibiting erythropoiesis through an IRF-1/PU.1 axisSten F Libregts
Department of Experimental Immunology, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands
Blood 118:2578-88. 2011..Notably, down-regulation of either IRF-1 or PU.1 expression is sufficient to overcome IFN-γ-induced inhibition of erythropoiesis. These findings reveal a molecular mechanism by which chronic exposure to IFN-γ induces anemia... - Increased cation conductance in human erythrocytes artificially aged by glycationYuliya V Kucherenko
Department of Physiology, Institute I, Eberhard Karls Universitat Tubingen, Gmelinstr 5, 72076, Tubingen, Germany
J Membr Biol 235:177-89. 2010..The present study explored whether glycation-induced erythrocyte aging is paralleled by features of suicidal erythrocyte death or eryptosis, which is characterized by cell .. - Enhanced erythro-phagocytosis in polycythemic mice overexpressing erythropoietinAnna Bogdanova
Institute of Veterinary Physiology, Vetsuisse Faculty and Zurich Center for Integrative Human Physiology ZIHP, University of Zurich, Switzerland
Blood 110:762-9. 2007..In conclusion, in tg6 mice erythrocyte aging is accelerated, which results, together with an increased number and activity of their macrophages, in .. - Fragmentation of human erythrocyte actin following exposure to hypoxiaAngela Risso
Departments of Biology and Plant Protection, M A T I Centre of Excellence, University of Udine, via delle Scienze 91, Udine, Italy
Acta Haematol 123:6-13. 2010..In conclusion, these results indicate that after exposure to hypoxia, RBCs display a modification of their actin and cytoskeleton instability... - Force balance and membrane shedding at the red-blood-cell surfacePierre Sens
Physico Chimie Théorique CNRS UMR 7083, ESPCI, 10 rue Vauquelin, 75231 Paris, France
Phys Rev Lett 98:018102. 2007..This model introduces the concept of force balance as a regulatory process at the cell membrane and quantitatively reproduces the rate of area loss in aging red-blood cells... - Adenosine protects against suicidal erythrocyte deathOlivier M Niemoeller
Department of Physiology, University of Tubingen, Tubingen, Germany
Pflugers Arch 454:427-39. 2007..d. at > or =10 microM). In conclusion, adenosine inhibits eryptosis by a mechanism presumably effective downstream of PKC. The effect may participate in the maintenance of microcirculation in ischemic tissue... - Shortened lifespan of paroxysmal nocturnal haemoglobinuria-affected RBC estimated from differences in ratios of CD59-negative populations between reticulocytes and whole RBCH Ninomiya
Institute of Clinical Medicine, University of Tsukuba, Tsukuba, Ibaraki, Japan
Int J Lab Hematol 30:41-5. 2008..The MLS, in individual patients, altered irrespective of RBC and percentage reticulocytes. The MLS calculated from our methods may be a parameter that evaluates the haemolytic conditions in PNH... - Angiogenic endothelium shows lactadherin-dependent phagocytosis of aged erythrocytes and apoptotic cellsMarcel H A M Fens
Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, The Netherlands
Blood 111:4542-50. 2008..Implications of our findings for diagnostic and therapy of angiogenesis-driven diseases are discussed... - Factor H mediated cell surface protection from complement is critical for the survival of PNH erythrocytesViviana P Ferreira
Department of Biochemistry, Center for Biomedical Research, University of Texas Health Science Center, Tyler, TX 75708, USA
Blood 110:2190-2. 2007..The results indicate that cells deficient in surface-bound regulators are protected for extended periods of time by factor H... - Simultaneous determination of cell aging and ATP release from erythrocytes and its implications in type 2 diabetesWasanthi Subasinghe
Department of Chemistry, Michigan State University, East Lansing, MI, USA
Anal Chim Acta 618:227-33. 2008..This determination provides evidence that antioxidant status in the RBC and its ability to release ATP, a known stimulus of nitric oxide production, are closely related... - Transgenic rescue of hemolytic anemia due to red blood cell pyruvate kinase deficiencyHitoshi Kanno
Department of Transfusion Medicine and Cell Processing, Tokyo Women s Medical University, Tokyo, Japan
Haematologica 92:731-7. 2007..To test whether the targeted overexpression of the normal R-PK gene in erythroid cells could reduce hemolysis in R-PK mutant mice, we performed a genetic rescue study using human R-PK transgenic mice... - The measurement and importance of red cell survivalRobert S Franco
Division of Hematology Oncology, University of Cincinnati College of Medicine, Cincinnati, Ohio 45267 0508, USA
Am J Hematol 84:109-14. 2009..In this review, measurement techniques are placed in historical perspective and examined for relative merits and suitable application... - Erythrocyte ageing in vivo and in vitro: structural aspects and implications for transfusionG J C G M Bosman
Department of Biochemistry, Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
Transfus Med 18:335-47. 2008.... - Stycholysin II, a cytolysin from the sea anemone Stichodactyla helianthus promotes higher hemolysis in aged red blood cellsGloria Celedón
Departamento de Fisiologia, Facultad de Ciencias, Universidad de Valparaiso, Chile
Toxicon 51:1383-90. 2008..It is concluded that this factor and the amount of intracellular K(+) are the dominant parameters that modulate the resistance of RBC to St II-induced lysis... - In vitro storage and in vivo survival studies of red cells from persons with the In(Lu) geneS K Ballas
Cardeza Foundation for Hematologic Research, Department of Medicine, Thomas Jefferson University Hospital Blood Bank, Philadelphia, Pennsylvania
Transfusion 32:607-11. 1992..Individuals of the Lu(a-b-) phenotype, associated with In(Lu), may not be suitable candidates for routine blood donation... - Improved storage of erythrocytes by prior leukodepletion: flow cytometric evaluation of stored erythrocytesD Bratosin
National Institute of Biological Science Research and Development, Bucharest, Rumania
Cytometry 46:351-6. 2001..Previously developed flow cytometric procedures to detect in vivo "RBC senescence" have been applied and proved to be reliable criteria to monitor the viability of stored RBCs... - In vivo survival of apheresis RBCs, frozen with 40-percent (wt/vol) glycerol, deglycerolized in the ACP 215, and stored at 4 degrees C in AS-3 for up to 21 daysC R Valeri
Naval Blood Research Laboratory, Boston University School of Medicine, 625 Albany Street, Boston, MA 02118, USA
Transfusion 41:928-32. 2001..The 4 degrees C postwash storage period is limited to 24 hours, because the current deglycerolization systems are functionally open systems... - Involvement of phosphatidylserine exposure in the recognition and phagocytosis of uremic erythrocytesM Bonomini
Department of Medicine, Institute of Nephrology, SS Annunziata Hospital, Via dei Vestini, 66013 Chieti Scalo, Italy
Am J Kidney Dis 37:807-14. 2001..These findings suggest that a PS-recognition mechanism may promote the susceptibility of uremic RBCs to phagocytosis and thus be involved in the shortened erythrocyte life span of uremia... - Reduced oxidative-stress response in red blood cells from p45NFE2-deficient miceJ Y Chan
Department of Laboratory Medicine, University of California, San Francisco 94143 0793, USA
Blood 97:2151-8. 2001..These findings suggest a role for p45NF-E2 in the oxidative-stress response in RBCs and indicate that p45NF-E2 deficiency contributes to the anemia in p45nf-e2(-/-) mice. (Blood. 2001;97:2151-2158).. - Rheological action of aspirin on human erythrocytesY Y Bilto
Department of Biological Sciences, University of Jordan, Amman
Clin Hemorheol Microcirc 20:159-65. 1999..The observed effects of aspirin could be explained by acetylation of intracellular proteins and hence saturation (concentration) of the cell interior with the osmotically active drug... - Calculation of a Gap restoration in the membrane skeleton of the red blood cell: possible role for myosin II in local repairC Cibert
Laboratoire de Biologie du Developpement, Institut Jacques Monod, UMR 7592, CNRS, Universités Paris VI et Paris VII, F 75005 Paris, France
Biophys J 76:1153-65. 1999..This may be involved in a more complex repair process of the red blood cell membrane, which includes the vesiculation of the bilayer and the compaction of the disassembled spectrin network... - An animal model to study erythrocyte senescence with a narrow time window of erythrocyte productionZ Y Wen
Department of Medical Physics, Beijing Medical University, P R China
Clin Hemorheol Microcirc 18:75-84. 1998..This indicates that the density gradient technique can be used to separate RBC population into fractions with different mean ages, but has a low resolving power for obtaining individual RBCs of a given "age"... - A new method to measure iron absorption from the enrichment of 57Fe and 58Fe in young erythroid cellsE G van den Heuvel
TNO Nutrition and Food Research Institute, AJ Zeist, The Netherlands
Clin Chem 44:649-54. 1998..0 +/- 3.3%). Because a threefold higher isotope enrichment was found in the cell fractions, the required dose of stable isotopes can be reduced to one-third of the dose used in the traditional method without loss of sensitivity... - Flow cytometric assessment of oxidant stress in age-fractionated thalassaemic trait erythrocytes and its relationship to in vitro growth of Plasmodium falciparumA C Senok
Department of Paediatrics, Chinese University of Hong Kong, Shatin NT, Hong Kong
Parasitology 116:1-6. 1998..Our findings indicate an association between red cell age, oxidant stress and P. falciparum growth, providing further support for the role of oxidant stress in mediating the protective effect against malaria in thalassaemic RBC... - Iron release, membrane protein oxidation and erythrocyte ageingC Signorini
Institute of General Pathology, University of Siena, Italy
FEBS Lett 362:165-70. 1995..The addition of ferrozine during the aerobic incubation prevented both the IgG binding and the protein alterations seen in the IR spectra, suggesting an intracellular chelation of the released iron by ferrozine... - The multiple functions of hemoglobinB Giardina
Istituto di Chimica e Chimica Clinica, Facolta di Medicina e Chirurgia, Universita Cattolica, Roma, Italy
Crit Rev Biochem Mol Biol 30:165-96. 1995.... - Red cell adenylate kinase deficiency associated with hereditary nonspherocytic hemolytic anemia: clinical and biochemical studiesS Miwa
Am J Hematol 14:325-33. 1983..The mode of inheritance of this case is obscure. The mechanism of hemolysis might be a structural gene mutation that caused altered electrophoretic and kinetic properties... - Posttransfusion survival of red cells frozen for 8 weeks after 42-day liquid storage in AS-3E J Rathbun
Department of Transfusion Medicine, Warren G Magnuson Clinical Center, Bethesda, Maryland
Transfusion 29:213-7. 1989..These findings show that an acceptable percentage of RBCs survives frozen storage after maximum liquid storage (mean ITE greater than 60%). If necessary, RBCs stored in AS-3 can be frozen at any time before 42 days... - Role of Se in stabilization of human erythrocyte membrane skeletonF Y Yang
Institute of Biophysics, Academia Sinica, Beijing, China
Biochem Int 15:475-82. 1987..However, since GSHPx is mainly distributed in the cytoplasm of erythrocytes, the stabilizing effect of Se on erythrocyte membranes might not be related to the activity of this enzyme... - Increase in Hb-O2-affinity at moderate altitude (2000 m) in patients on maintenance hemodialysisH Mairbaurl
Institute of Physiology, University of Innsbruck, Austria
Clin Nephrol 31:198-203. 1989..We interpret the increase of the oxygen affinity of hemoglobin in patients with renal anemia as beneficial, as it favors oxygen loading of hemoglobin in the lung during exposure to a hypoxic environment... - Significant calcium accumulation in fragile erythrocytes by dietary restriction in miceK Hishinuma
Faculty of Agriculture, Tohoku University, Sendai, Nippon
Int J Vitam Nutr Res 60:372-8. 1990..of the erythrocytes, the oxygen supply to the peripheral tissues, may be maintained as 2,3-bisphosphoglyceric acid levels were not altered with erythrocyte aging in spite of the decreased glycolytic activity in the diet restricted mice. - The effect of ammonium, phosphate, potassium, and hypotonicity on stored red cellsA Kay
Department of Molecular and Experimental Medicine, Scripps Clinic and Research Foundation, La Jolla, California
Transfusion 32:37-41. 1992..Despite prolonged ATP maintenance by ammonium and phosphate-containing storage media, satisfactory viability of RBCs stored up to 77 days was not demonstrated in the rabbit transfusion model... - Osmotic resistance of high-density erythrocytes in transglutaminase 2-deficient miceFrancesca Bernassola
Biochemistry Laboratory, c o Department of Experimental Medicine, University of Rome Tor Vergata, Via Tor Vergata 135, 00133 Rome, Italy
Biochem Biophys Res Commun 291:1123-7. 2002..The Ca(2+)-dependent TGase 2-dependent cross-linking activity has been proposed for its involvement in erythrocyte aging, by inducing irreversible modification of their cell shape and deformability... - In vivo studies of monoclonal anti-D and the mechanism of immune suppressionBelinda M Kumpel
International Blood Group Reference Laboratory, Bristol Institute of Transfusion Sciences, Southmead Road, Bristol BS10 5ND, UK
Transfus Clin Biol 9:9-14. 2002..The most likely mechanism of action was considered to be inhibition of B cells resulting from co-crosslinking antigen receptors with inhibitory Fc gamma R when the B cells contacted red cells that had bound passive anti-D... - Aluminum hemotoxicity mechanismsElzbieta Osińska
Institute of Rural Medicine, Lublin
Ann Univ Mariae Curie Sklodowska Med 59:411-6. 2004..A number of processes, namely aluminum's hemolytic activity, blood cells' shorter lifetime or disturbed erythropoiesis process, are responsible for hematological changes... - SHPS-1 promotes the survival of circulating erythrocytes through inhibition of phagocytosis by splenic macrophagesTomomi Ishikawa-Sekigami
Department of Medicine and Clinical Scinece, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan
Blood 107:341-8. 2006..These results suggest that SHPS-1 negatively regulates the phagocytosis of RBCs by splenic macrophages, thereby determining both the lifespan of individual RBCs and the number of circulating erythrocytes... - Methionine oxidation and agingEarl R Stadtman
Laboratory of Biochemistry, National Heart, Lung and Blood Institute, Bethesda, MD 20892, USA
Biochim Biophys Acta 1703:135-40. 2005..of proteins increases with age in a number of different aging models, including replicative senescence and erythrocyte aging, but not in mouse tissues during aging...
Research Grants
- Defining the IsoAspartomeCheng Lin; Fiscal Year: 2010..presence of isoAsp in a wide variety of systems from in vitro peptides to whole tumor cells to studies of erythrocyte aging. Finally, this study will apply these methods to detailed study of isoaspartic acid formation rates via both ..
- Defining the IsoAspartomePETER O CONNOR; Fiscal Year: 2007..presence of isoAsp in a wide variety of systems from in vitro peptides to whole tumor cells to studies of erythrocyte aging. Finally, this study will apply these methods to detailed study of isoaspartic acid formation rates via both ..
- Reducing Mortality from Acute Hemorrhage in TraumaJohn Hess; Fiscal Year: 2006..Reducing death from acute hemorrhage in trauma care will be a major step forward in reducing overall trauma mortality. ..
- IN UTERO STEM CELL TRANSFER IN MPS VIIJane Barker; Fiscal Year: 2004..abstract_text> ..
- LY-1 B CELLS--DEVELOPMENTAL ORIGINS AND V GENERichard Hardy; Fiscal Year: 2000..Cumulatively, these studies will provide a clearer picture of fetal and adult B lymphopoiesis and distinguish whether the modes of selection affecting developing B cells are similar or different during these two ontogenic timi s. ..
- IMMUNOGLOBULIN V GENES AND B CELL DEVELOPMENTRichard Hardy; Fiscal Year: 2006..The results of these studies will help to establish the types of selection that shape the expressed B cell repertoire, both fetally and in the adult, with implications for modulating normal and autoimmune responses. ..
- LY-1 B CELLS: DEVELOPMENTAL ORIGINS AND V GENE BIASESRichard Hardy; Fiscal Year: 2007....
- GENETIC ENGINEERING OF CELLS FROM ANEMIC ANIMALSJane Barker; Fiscal Year: 2003..abstract_text> ..
- IMMUNOGLOBULIN V GENES AND B CELL DEVELOPMENTRichard Hardy; Fiscal Year: 2001..The results of these studies will help to establish the types of selection that shape the expressed B cell repertoire, fetally and in the adult, with implications for modulating both normal and autoimmune responses. ..
- Hemolytic Anemia: ModelsJane Barker; Fiscal Year: 2004..5 isoform in the structure and assembly of the sarcoplasmic reticulum by an in vivo developmental study. ..
- LY-1 B CELLS: DEVELOPMENTAL ORIGINS AND V GENE BIASESRichard Hardy; Fiscal Year: 2005..abstract_text> ..
- IN UTERO STEM CELL TRANSFER IN MPS VIIJane Barker; Fiscal Year: 2002....
- BD Biosciences LSR Flow CytometerRichard Hardy; Fiscal Year: 2002..abstract_text> ..
- HEMOLYTIC ANEMIAS--MODELSJane Barker; Fiscal Year: 2002..abstract_text> ..
- HEMOLYTIC ANEMIAS--MODELSJane Barker; Fiscal Year: 1993....
- HEMOLYTIC ANEMIAS MODELSJane Barker; Fiscal Year: 1990....
- Pulmonary Hypertension in ThalassemiaSYLVIA SINGER; Fiscal Year: 2007....
- LY-1 B CELLS--LINEAGE DETERMINATION/ANTIBODY REPERTOIRERichard Hardy; Fiscal Year: 1992..Specificities encoded by these V genes to be examined in detail will include both anti-thymocyte and anti-bromelain-treated mouse erythrocyte autoantibodies, both distinctively enriched in this population...
- FACSVantage SE Sorting Flow CytometerJane Barker; Fiscal Year: 2002..Funds are requested to purchase the FACSVantage SE and the optional equipment for TJL's Flow Cytometry Service. This will provide a cost-effective way to fill an urgent need of TJL's principal investigators. ..