cell shape


Summary: The quality of surface form or outline of CELLS.

Top Publications

  1. pmc PGE(2) inhibition of TGF-beta1-induced myofibroblast differentiation is Smad-independent but involves cell shape and adhesion-dependent signaling
    Peedikayil E Thomas
    Department of Internal Medicine, Division of Pulmonary and Critical Care Medicine, University of Michigan, Ann Arbor, MI 48109 2200, USA
    Am J Physiol Lung Cell Mol Physiol 293:L417-28. 2007
  2. pmc Actin, a central player in cell shape and movement
    Thomas D Pollard
    Department of Molecular, Cellular and Developmental Biology, Yale University, Post Office Box 208103, New Haven, CT 06520 8103, USA
    Science 326:1208-12. 2009
  3. ncbi Tensional homeostasis and the malignant phenotype
    Matthew J Paszek
    Department of Bioengineering, University of Pennsylvania, Philadelphia, 19104, USA
    Cancer Cell 8:241-54. 2005
  4. ncbi Matrix elasticity directs stem cell lineage specification
    Adam J Engler
    Pennsylvania Muscle Institute, University of Pennsylvania, Philadelphia, 19104, USA
    Cell 126:677-89. 2006
  5. pmc Coordination of Rho GTPase activities during cell protrusion
    Matthias Machacek
    Department of Cell Biology, The Scripps Research Institute, 10550 N Torrey Pines Road, La Jolla, California 92037, USA
    Nature 461:99-103. 2009
  6. ncbi Adhesion signaling - crosstalk between integrins, Src and Rho
    Stephan Huveneers
    Division of Toxicology, Leiden Amsterdam Center for Drug Research, Leiden University, 2300 RA Leiden, The Netherlands
    J Cell Sci 122:1059-69. 2009
  7. pmc Vimentin induces changes in cell shape, motility, and adhesion during the epithelial to mesenchymal transition
    Melissa G Mendez
    Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA
    FASEB J 24:1838-51. 2010
  8. pmc Peptidoglycan-modifying enzyme Pgp1 is required for helical cell shape and pathogenicity traits in Campylobacter jejuni
    Emilisa Frirdich
    Department of Microbiology and Immunology, University of British Columbia, Vancouver, British Columbia, Canada
    PLoS Pathog 8:e1002602. 2012
  9. pmc Integration of contractile forces during tissue invagination
    Adam C Martin
    Department of Molecular Biology, Howard Hughes Medical Institute, Princeton University, Princeton, NJ 08544, USA
    J Cell Biol 188:735-49. 2010
  10. ncbi Hydrostatic pressure and the actomyosin cortex drive mitotic cell rounding
    Martin P Stewart
    ETH Zurich, Department of Biosystems Science and Engineering, CH 4058 Basel, Switzerland
    Nature 469:226-30. 2011

Detail Information

Publications278 found, 100 shown here

  1. pmc PGE(2) inhibition of TGF-beta1-induced myofibroblast differentiation is Smad-independent but involves cell shape and adhesion-dependent signaling
    Peedikayil E Thomas
    Department of Internal Medicine, Division of Pulmonary and Critical Care Medicine, University of Michigan, Ann Arbor, MI 48109 2200, USA
    Am J Physiol Lung Cell Mol Physiol 293:L417-28. 2007
    ..Rather, PGE(2) has dramatic effects on cell shape and cytoskeletal architecture and disrupts the formation of appropriate focal adhesions...
  2. pmc Actin, a central player in cell shape and movement
    Thomas D Pollard
    Department of Molecular, Cellular and Developmental Biology, Yale University, Post Office Box 208103, New Haven, CT 06520 8103, USA
    Science 326:1208-12. 2009
    ..Comparisons of quantitative measurements of reactions in live cells with computer simulations of mathematical models will also help generate meaningful insights...
  3. ncbi Tensional homeostasis and the malignant phenotype
    Matthew J Paszek
    Department of Bioengineering, University of Pennsylvania, Philadelphia, 19104, USA
    Cancer Cell 8:241-54. 2005
    ..Thus, ERK and Rho constitute part of an integrated mechanoregulatory circuit linking matrix stiffness to cytoskeletal tension through integrins to regulate tissue phenotype...
  4. ncbi Matrix elasticity directs stem cell lineage specification
    Adam J Engler
    Pennsylvania Muscle Institute, University of Pennsylvania, Philadelphia, 19104, USA
    Cell 126:677-89. 2006
    ..The results have significant implications for understanding physical effects of the in vivo microenvironment and also for therapeutic uses of stem cells...
  5. pmc Coordination of Rho GTPase activities during cell protrusion
    Matthias Machacek
    Department of Cell Biology, The Scripps Research Institute, 10550 N Torrey Pines Road, La Jolla, California 92037, USA
    Nature 461:99-103. 2009
  6. ncbi Adhesion signaling - crosstalk between integrins, Src and Rho
    Stephan Huveneers
    Division of Toxicology, Leiden Amsterdam Center for Drug Research, Leiden University, 2300 RA Leiden, The Netherlands
    J Cell Sci 122:1059-69. 2009
    ..We also discuss how this signaling network can regulate a range of cellular processes that are important for normal tissue function and disease, including cell adhesion, spreading, migration and mechanotransduction...
  7. pmc Vimentin induces changes in cell shape, motility, and adhesion during the epithelial to mesenchymal transition
    Melissa G Mendez
    Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA
    FASEB J 24:1838-51. 2010
    ..cell lines, we show that the level of expression of vimentin IFs (VIFs) correlates with mesenchymal cell shape and motile behavior...
  8. pmc Peptidoglycan-modifying enzyme Pgp1 is required for helical cell shape and pathogenicity traits in Campylobacter jejuni
    Emilisa Frirdich
    Department of Microbiology and Immunology, University of British Columbia, Vancouver, British Columbia, Canada
    PLoS Pathog 8:e1002602. 2012
    ..jejuni gene shown to be involved in maintenance of C. jejuni cell shape. Pgp1 contributes to key pathogenic and cell envelope phenotypes...
  9. pmc Integration of contractile forces during tissue invagination
    Adam C Martin
    Department of Molecular Biology, Howard Hughes Medical Institute, Princeton University, Princeton, NJ 08544, USA
    J Cell Biol 188:735-49. 2010
    ..Drosophila mesoderm invagination, pulsed actomyosin meshwork contractions and a ratchet-like stabilization of cell shape drive apical constriction. Here, we investigate how contractile forces are integrated across the tissue...
  10. ncbi Hydrostatic pressure and the actomyosin cortex drive mitotic cell rounding
    Martin P Stewart
    ETH Zurich, Department of Biosystems Science and Engineering, CH 4058 Basel, Switzerland
    Nature 469:226-30. 2011
    ..Thus, by locally modulating actomyosin-cortex-dependent surface tension and globally regulating osmotic pressure, cells can control their volume, shape and mechanical properties...
  11. ncbi Developmental patterning by mechanical signals in Arabidopsis
    Olivier Hamant
    INRA, Laboratoire de Reproduction et Développement des Plantes, 46 allee d Italie, 69364 Lyon Cedex 07, France
    Science 322:1650-5. 2008
  12. pmc Inhibition of platelet-derived growth factor receptor signaling regulates Oct4 and Nanog expression, cell shape, and mesenchymal stem cell potency
    Stephen G Ball
    Wellcome Trust Centre for Cell Matrix Research, Faculty of Life Sciences, University of Manchester, Manchester, UK
    Stem Cells 30:548-60. 2012
    ..In these PDGFR inhibitor-treated MSCs, Oct4 and Nanog expression and cell shape were regulated by janus kinase (JAK), MAPK kinase (MEK), and epidermal growth factor receptor (EGFR) signaling...
  13. ncbi Local force and geometry sensing regulate cell functions
    Viola Vogel
    Laboratory for Biologically Oriented Materials, Department of Materials, Swiss Federal Institute of Technology ETH, Zurich, Wolfgang Pauli Strasse 10, ETH Honggerberg, HCI F443, CH 8093 Zurich, Switzerland
    Nat Rev Mol Cell Biol 7:265-75. 2006
    ..Recent tissue scaffolds that have been engineered at the micro- and nanoscale level now enable better dissection of the mechanosensing, transduction and response mechanisms...
  14. ncbi Reprogramming of human somatic cells to pluripotency with defined factors
    In Hyun Park
    Division of Pediatric Hematology Oncology, Children s Hospital Boston and Dana Farber Cancer Institute, Boston, Massachusetts 02115, USA
    Nature 451:141-6. 2008
    ..These data demonstrate that defined factors can reprogramme human cells to pluripotency, and establish a method whereby patient-specific cells might be established in culture...
  15. pmc Apical constriction: a cell shape change that can drive morphogenesis
    Jacob M Sawyer
    Biology Department, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Dev Biol 341:5-19. 2010
    ..b>Cell shape changes and tissue movements like these are at the core of many of the morphogenetic movements that shape animal ..
  16. ncbi Molecular mechanisms of cell shape changes that contribute to vertebrate neural tube closure
    Makoto Suzuki
    Division of Morphogenesis, Department of Developmental Biology, National Institute for Basic Biology, Nishigonaka 38, Myodaiji, Okazaki, 444 8585, Aichi, Japan
    Dev Growth Differ 54:266-76. 2012
    ..widely accepted that spatial and temporal changes of the cytoskeletal organization are fundamental to epithelial cell shape changes, and that noncetrosomal microtubules assembled along apicobasal axis and actin filaments and non-muscle ..
  17. pmc Changes in Ect2 localization couple actomyosin-dependent cell shape changes to mitotic progression
    Helen K Matthews
    MRC Laboratory for Molecular Cell Biology, University College London, Gower St, London WC1E 6BT, UK
    Dev Cell 23:371-83. 2012
    ..Ect2 localization therefore defines the stage-specific changes in actin cortex organization critical for accurate cell division...
  18. pmc Apical domain polarization localizes actin-myosin activity to drive ratchet-like apical constriction
    Frank M Mason
    Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02142, USA
    Nat Cell Biol 15:926-36. 2013
    ..The transcription factor Twist is required to stabilize cell shape. However, it is unknown how Twist spatially coordinates downstream signals to prevent cell relaxation...
  19. pmc An adhesion-dependent switch between mechanisms that determine motile cell shape
    Erin L Barnhart
    Department of Biochemistry and Howard Hughes Medical Institute, Stanford School of Medicine, Stanford, California, United States of America
    PLoS Biol 9:e1001059. 2011
  20. pmc RhoA and RhoC have distinct roles in migration and invasion by acting through different targets
    Francisco M Vega
    Randall Division of Cell and Molecular Biophysics, King s College London, London SE1 1UL, England, UK
    J Cell Biol 193:655-65. 2011
    ..We propose that RhoA contributes to migratory cell polarity through ROCK2-mediated suppression of Rac1 activity in lamellipodia, whereas RhoC promotes polarized migration through FMNL3 by restricting lamellipodial broadening...
  21. pmc Cyclic stress at mHz frequencies aligns fibroblasts in direction of zero strain
    Uta Faust
    Institute of Complex Systems, ICS 7, Biomechanics, Forschungszentrum Julich GmbH, Julich, Germany
    PLoS ONE 6:e28963. 2011
    ..Cyclic stretch increases the number of stress fibers and the coupling to adhesions. We show that changes in cell shape follow cytoskeletal reorientation with a significant temporal delay...
  22. pmc Aging of mesenchymal stem cell in vitro
    Mandana Mohyeddin Bonab
    Hematology Oncology and BMT Research Center, Shariati Hospital, Tehran University of Medical Sciences, Iran
    BMC Cell Biol 7:14. 2006
    ..The low frequency of this subpopulation of stem cells in bone marrow (BM) necessitates their in vitro expansion prior to clinical use. We evaluated the effect of long term culture on the senescence of these cells...
  23. ncbi Polar actomyosin contractility destabilizes the position of the cytokinetic furrow
    Jakub Sedzinski
    Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany
    Nature 476:462-6. 2011
    ..Whether polar forces influence cytokinetic cell shape and furrow positioning remains an open question...
  24. ncbi Control of Drosophila gastrulation by apical localization of adherens junctions and RhoGEF2
    Verena Kölsch
    Institute of Genetics, University of Cologne, Zulpicher Strasse 47, 50674 Cologne, Germany
    Science 315:384-6. 2007
    ..Together with G protein signaling, T48 recruits adherens junctions and the cytoskeletal regulator RhoGEF2 to the sites of apical constriction, ensuring rapid and intense changes in cell shape.
  25. pmc Triggering a cell shape change by exploiting preexisting actomyosin contractions
    Minna Roh-Johnson
    Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Science 335:1232-5. 2012
    ..We found that apical actomyosin contractions began before cell shape changes in both Caenorhabitis elegans and Drosophila. In C...
  26. ncbi folded gastrulation, cell shape change and the control of myosin localization
    Rachel E Dawes-Hoang
    Department of Molecular Biology, Howard Hughes Medical Institute, Princeton University, NJ 08544, USA
    Development 132:4165-78. 2005
    ..However, little is known about how patterning genes influence cytoarchitecture to drive changes in cell shape. In this paper, we analyze the function of the folded gastrulation gene (fog), a known target of the patterning ..
  27. ncbi Morphogenetic cell movements: diversity from modular mechanical properties
    Denise J Montell
    Department of Biological Chemistry, Center for Cell Dynamics, Rangos Building, Suite 450, 855 North Wolfe Street, Baltimore, MD 21205, USA
    Science 322:1502-5. 2008
    ..A small number of properties used in combination could, in principle, generate a diverse array of cell shapes and arrangements and thus orchestrate the varied morphogenetic events observed during metazoan organ development...
  28. pmc Stem cell shape regulates a chondrogenic versus myogenic fate through Rac1 and N-cadherin
    Lin Gao
    Department of Bioengineering, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Stem Cells 28:564-72. 2010
    ..Our previous work revealed that cell shape regulates hMSC commitment between osteoblasts and adipocytes through RhoA signaling...
  29. ncbi Cell distribution of stress fibres in response to the geometry of the adhesive environment
    Manuel Thery
    Biologie du cycle cellulaire et de la motilité, UMR144, CNRS, Institut Curie, Paris, France
    Cell Motil Cytoskeleton 63:341-55. 2006
    ..Adhesive micropatterns are commonly used to confine cell shape within a given geometry...
  30. ncbi Metabolism and cell shape in cancer: a fractal analysis
    Fabrizio D'Anselmi
    ASI, Italian Space Agency, Roma, Italy
    Int J Biochem Cell Biol 43:1052-8. 2011
    ..Some reports demonstrated that changes in cell shape precede and trigger dramatic modifications in both gene expression and enzymatic function...
  31. pmc A contractile actomyosin network linked to adherens junctions by Canoe/afadin helps drive convergent extension
    Jessica K Sawyer
    Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Mol Biol Cell 22:2491-508. 2011
    ..Germband cells have an apical actomyosin network undergoing cyclical contractions. These coincide with a novel cell shape change--cell extension along the anterior-posterior (AP) axis. In Canoe's absence, GBE is disrupted...
  32. ncbi Physical forces and non linear dynamics mould fractal cell shape: quantitative morphological parameters and cell phenotype
    M Bizzarri
    Department of Experimental Medicine, Sapienza University of Rome, Rome, Italy
    Histol Histopathol 28:155-74. 2013
    b>Cell shape is mainly determined by biophysical constraints, interacting according to non-linear dynamics upon the basic units provided by the genome...
  33. pmc Coupling actin flow, adhesion, and morphology in a computational cell motility model
    Danying Shao
    Center for Theoretical Biological Physics and Department of Physics, University of California, San Diego, La Jolla, CA 92093 0374, USA
    Proc Natl Acad Sci U S A 109:6851-6. 2012
    ..Our model suggests that the pattern of the actin flow inside the cell, the cell velocity, and the cell morphology are determined by the integration of actin polymerization, myosin contraction, adhesion forces, and membrane forces...
  34. pmc Theoretical model for cellular shapes driven by protrusive and adhesive forces
    Doron Kabaso
    Department of Chemical Physics, The Weizmann Institute of Science, Rehovot, Israel
    PLoS Comput Biol 7:e1001127. 2011
    The forces that arise from the actin cytoskeleton play a crucial role in determining the cell shape. These include protrusive forces due to actin polymerization and adhesion to the external matrix...
  35. pmc Bacterial actin MreB assembles in complex with cell shape protein RodZ
    Fusinita van den Ent
    MRC Laboratory of Molecular Biology, Cambridge, UK
    EMBO J 29:1081-90. 2010
    Bacterial actin homologue MreB is required for cell shape maintenance in most non-spherical bacteria, where it assembles into helical structures just underneath the cytoplasmic membrane...
  36. pmc Cultivation of human corneal endothelial cells isolated from paired donor corneas
    Gary S L Peh
    Singapore Eye Research Institute, Singapore, Singapore
    PLoS ONE 6:e28310. 2011
  37. pmc Cell shape and contractility regulate ciliogenesis in cell cycle-arrested cells
    Amandine Pitaval
    Laboratoire Biopuces, Institut de Recherche en Sciences et Technologies pour le Vivant, Direction des Sciences du Vivant, Commissariat à l Énergie Atomique et aux Énergies Alternatives, 38054 Grenoble, Cedex 09, France
    J Cell Biol 191:303-12. 2010
    ..These results indicate that cell geometrical confinement affects cell polarity via the modulation of actin network architecture and thereby regulates basal body positioning and primary cilium growth...
  38. ncbi Cell surface mechanics and the control of cell shape, tissue patterns and morphogenesis
    Thomas Lecuit
    Institute of Developmental Biology of Marseille Luminy, UMR6216 CNRS Université de la Méditerranée, Campus de Luminy Case 907, 13288 Marseille Cedex 09, France
    Nat Rev Mol Cell Biol 8:633-44. 2007
    ..However, how this information is processed to control cell shape and cell dynamics is an open area of investigation...
  39. pmc Bone morphogenetic protein-2-induced signaling and osteogenesis is regulated by cell shape, RhoA/ROCK, and cytoskeletal tension
    Yang Kao Wang
    Department of Bioengineering, University of Pennsylvania, Philadelphia, PA 19104, USA
    Stem Cells Dev 21:1176-86. 2012
    ..Here, we report that cell adhesion to extracellular matrix (ECM), and its effects on cell shape and cytoskeletal mechanics, regulates BMP-induced signaling and osteogenic differentiation of hMSCs...
  40. pmc Cell shape and substrate rigidity both regulate cell stiffness
    Shang You Tee
    Institute for Medicine and Engineering, University of Pennsylvania, Philadelphia, Pennsylvania, USA
    Biophys J 100:L25-7. 2011
    ..It is currently unknown how substrate stiffness, cell shape, and cell stiffness modulate or interact with one another...
  41. pmc Force transmission in migrating cells
    Maxime F Fournier
    Laboratoire de Biophysique Cellulaire, Ecole Polytechnique Federale de Lausanne, CH 1015 Lausanne, Switzerland
    J Cell Biol 188:287-97. 2010
  42. pmc The shape of motile cells
    Alex Mogilner
    Department of Neurobiology, Physiology and Behavior, University of California, Davis, CA 95616, USA
    Curr Biol 19:R762-71. 2009
    ..We start with geometric rules describing cell-edge kinetics that govern cell shape, followed by a discussion of the underlying biophysics; we consider actin treadmilling, actin-myosin contraction,..
  43. ncbi Dynamic coordination of cytoskeletal and cell wall systems during plant cell morphogenesis
    Daniel B Szymanski
    Department of Agronomy, Lily Hall of Life Sciences, 915 West State Street, Purdue University, West Lafayette, IN 47907, USA
    Curr Biol 19:R800-11. 2009
    ..the mechanisms by which the cellular growth machinery and mechanical properties of the cell interact to dictate cell shape. The recent combined use of mutants, genomic analyses, sophisticated spectroscopies, and live cell imaging is ..
  44. ncbi Local positive feedback regulation determines cell shape in root hair cells
    Seiji Takeda
    Department of Cell and Developmental Biology, John Innes Centre, Norwich NR4 7UH, UK
    Science 319:1241-4. 2008
    ..Because the location and stability of growth sites predict the ultimate form of a plant cell, our findings demonstrate how a positive feedback mechanism involving RHD2, ROS, and Ca2+ can determine cell shape.
  45. pmc Enabled and Capping protein play important roles in shaping cell behavior during Drosophila oogenesis
    Julie Gates
    Lineberger Comprehensive Cancer Center and Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Dev Biol 333:90-107. 2009
    ..Together these data reveal places that these actin regulators shape oogenesis...
  46. ncbi Planar cell polarity pathway regulates actin rearrangement, cell shape, motility, and nephrin distribution in podocytes
    Sima Babayeva
    Department of Medicine, Nephrology Research Group, McGill University, Montreal, Quebec, Canada
    Am J Physiol Renal Physiol 300:F549-60. 2011
    ..Our observations indicate that PCP signaling may play an important role both in podocyte development and FP cytoskeleton dynamics...
  47. ncbi The effect of mesenchymal stem cell shape on the maintenance of multipotency
    Douglas Zhang
    Department of Materials Science and Engineering, University of Illinois at Urbana Champaign, 1304 W Green St, Urbana, IL 61801, USA
    Biomaterials 34:3962-9. 2013
    ..This work will prove useful in the development of culture conditions for the maintenance of multipotent MSCs in vitro and for the design of niche-mimetic biomaterials...
  48. ncbi Oscillatory behaviors and hierarchical assembly of contractile structures in intercalating cells
    Rodrigo Fernandez-Gonzalez
    Howard Hughes Medical Institute, Developmental Biology Program, Sloan Kettering Institute, 1275 York Avenue, New York, NY 10065, USA
    Phys Biol 8:045005. 2011
    ..with actin localization preceding myosin II and with the localization of both proteins preceding changes in cell shape. We discuss models to explain how the architecture of cytoskeletal networks regulates their contractile behavior ..
  49. ncbi Growth stimulation leads to cellular senescence when the cell cycle is blocked
    Zoya N Demidenko
    Oncotarget, Albany, New York, USA
    Cell Cycle 7:3355-61. 2008
    ..Inhibition of TOR partially prevented senescent phenotype caused by DOX. Thus growth stimulation coupled with cell cycle arrest leads to senescence, whereas quiescence (a condition with inactive TOR) prevents senescence...
  50. ncbi Dynamics of actomyosin contractile activity during epithelial morphogenesis
    Nicole Gorfinkiel
    Centro de Biologia Molecular Severo Ochoa, CSIC UAM, Cantoblanco 28049, Madrid, Spain
    Curr Opin Cell Biol 23:531-9. 2011
    ..and quantitative image analysis have lead to a completely new understanding of the processes underlying the cell shape changes and cell rearrangements that drive tissue morphogenesis...
  51. pmc Cytoskeletal and focal adhesion influences on mesenchymal stem cell shape, mechanical properties, and differentiation down osteogenic, adipogenic, and chondrogenic pathways
    Pattie S Mathieu
    Joint Department of Biomedical Engineering, University of North Carolina at Chapel Hill and North Carolina State University, Raleigh, NC, USA
    Tissue Eng Part B Rev 18:436-44. 2012
    ..Different mechanical stimuli can be implemented to alter these cytoskeletal patterns and encourage MSC differentiation to the desired lineage...
  52. ncbi Shroom regulates epithelial cell shape via the apical positioning of an actomyosin network
    Jeffrey D Hildebrand
    Department of Biological Sciences, 4249 Fifth Avenue, Crawford Hall, University of Pittsburgh, Pittsburgh, PA 15260, USA
    J Cell Sci 118:5191-203. 2005
    ..These events likely mimic the cell shape changes and cellular movements required for neurulation in vivo...
  53. pmc Balanced Rac1 and RhoA activities regulate cell shape and drive invagination morphogenesis in epithelia
    Bharesh K Chauhan
    Division of Pediatric Ophthalmology, Cincinnati Children s Hospital Medical Center, Cincinnati, OH 45229, USA
    Proc Natl Acad Sci U S A 108:18289-94. 2011
    ..Here we show that mutual antagonism by the small Rho GTPases Rac1 and RhoA determines cell shape, tissue curvature, and invagination activity in the model epithelium of the developing mouse lens...
  54. ncbi Pulsation and stabilization: contractile forces that underlie morphogenesis
    Adam C Martin
    Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    Dev Biol 341:114-25. 2010
    Embryonic development involves global changes in tissue shape and architecture that are driven by cell shape changes and rearrangements within cohesive cell sheets...
  55. ncbi Cell shape, cell-cell contact, cell-extracellular matrix contact and cell polarity are all required for the maximum induction of CYP2B1 and CYP2B2 gene expression by phenobarbital in adult rat cultured hepatocytes
    Hiroaki Oda
    Laboratory of Nutritional Biochemistry, Department of Applied Molecular Biosciences, Nagoya University, Nagoya 464 8601, Japan
    Biochem Pharmacol 75:1209-17. 2008
    The effect of cell shape, cell density, contact with extracellular matrix and cell polarity on the phenobarbital (PB)-induced gene expression of CYP2B1 and CYP2B2 (CYP2B1/2B2) in adult rat hepatocytes was investigated...
  56. pmc The regulation of traction force in relation to cell shape and focal adhesions
    Andrew D Rape
    Department of Biomedical Engineering, Carnegie Mellon University, PTC 4105 07, 700 Technology Drive, Pittsburgh, PA 15219, USA
    Biomaterials 32:2043-51. 2011
    ..A similar positive feedback control may allow cells to respond to a variety of physical or topographical signals via a unified mechanism...
  57. pmc Emergence of large-scale cell morphology and movement from local actin filament growth dynamics
    Catherine I Lacayo
    Department of Biochemistry, Stanford University, Stanford, California, United States of America
    PLoS Biol 5:e233. 2007
    ..of natural cell-to-cell variation within a population by using measurable morphological and behavioral features--cell shape, leading-edge shape, filamentous actin (F-actin) distribution, cell speed, and directional persistence--that we ..
  58. ncbi Cell migration during morphogenesis
    Andy Aman
    University of Utah, Department Neurobiology and Anatomy, 20N Medical Drive, MREB 401, Salt Lake City, UT 84132, USA
    Dev Biol 341:20-33. 2010
    ..More importantly, we will highlight, compare and integrate recent advances in our understanding of how cell migration is regulated in these varied model systems with special emphasis on individual and collective cell movements...
  59. ncbi Cytoskeletal dynamics and supracellular organisation of cell shape fluctuations during dorsal closure
    Guy B Blanchard
    Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge, UK
    Development 137:2743-52. 2010
    ..Our results suggest that cell shape fluctuations are a property of cells with low and increasing levels of apical myosin, and that medial and ..
  60. ncbi Cell shape changes indicate a role for extrinsic tensile forces in Drosophila germ-band extension
    Lucy C Butler
    Department of Physiology, Development and Neuroscience, University of Cambridge, UK
    Nat Cell Biol 11:859-64. 2009
    ..cell behaviours underlying tissue morphogenesis, we have continuously quantified cell intercalation and cell shape change during GBE...
  61. pmc Direct comparisons of the morphology, migration, cell adhesions, and actin cytoskeleton of fibroblasts in four different three-dimensional extracellular matrices
    Kirsi M Hakkinen
    Laboratory of Cell and Developmental Biology, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892 4370, USA
    Tissue Eng Part A 17:713-24. 2011
    ..This identification of quantitative differences in cellular responses to different matrix composition and dimensionality should help guide the development of customized 3D tissue culture and matrix scaffolds for tissue engineering...
  62. pmc Mitosis-specific mechanosensing and contractile-protein redistribution control cell shape
    Janet C Effler
    Department of Cell Biology, Johns Hopkins University School of Medicine, 725 N Wolfe Street, Baltimore, Maryland 21205, USA
    Curr Biol 16:1962-7. 2006
    ..Our data provide evidence for a mechanosensory system that directs contractile proteins to regulate cell shape during mitosis.
  63. ncbi Dpp signaling promotes the cuboidal-to-columnar shape transition of Drosophila wing disc epithelia by regulating Rho1
    Thomas J Widmann
    Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstr 108, 01307 Dresden, Germany
    J Cell Sci 122:1362-73. 2009
    Morphogenesis is largely driven by changes in the shape of individual cells. However, how cell shape is regulated in developing animals is not well understood...
  64. pmc Ordered patterns of cell shape and orientational correlation during spontaneous cell migration
    Yusuke T Maeda
    Department of Physics, Graduate School of Science, The University of Tokyo, Tokyo, Japan
    PLoS ONE 3:e3734. 2008
    ..Yet little is known about how cells change their own shape and how cells coordinate the deformation and movement. Here, we investigated the mechanism of spontaneous cell migration by using computational analyses...
  65. pmc Rho1 has multiple functions in Drosophila wing planar polarity
    Jie Yan
    Biology Department, Department of Cell Biology, Morphogenesis and Regenerative Medicine Institute and Cancer Center, University of Virginia, Charlottesville, VA 22903, USA
    Dev Biol 333:186-99. 2009
    ..Aberrant Rho1 activity led to changes in the number of hairs formed, changes in cell shape and F-actin and changes in cellular junctions...
  66. pmc Analysis of aquaporin-mediated diffusional water permeability by coherent anti-stokes Raman scattering microscopy
    Keiji Ibata
    Laboratory for Cell Function Dynamics, Brain Science Institute, The Institute of Physical and Chemical Research RIKEN, Saitama, Japan
    Biophys J 101:2277-83. 2011
    ..These results suggest the possibility of using CARS imaging to investigate the hydrodynamics of single mammalian cells as well as the regulation of AQPs...
  67. pmc Identification and characterization of a set of conserved and new regulators of cytoskeletal organization, cell morphology and migration
    Siau Wei Baï
    Randall Division of Cell and Molecular Biophysics, King s College London, New Hunt s House, Guy s Campus, London SE11UL, UK
    BMC Biol 9:54. 2011
    ..Cell migration is essential during development and in human disease progression including cancer. Most cell migration studies concentrate on known or predicted components of migration pathways...
  68. ncbi Dpp signalling orchestrates dorsal closure by regulating cell shape changes both in the amnioserosa and in the epidermis
    Beatriz García Fernández
    Instituto Gulbenkian de Ciencia, Rua da Quinta Grande 6, 2780 156 Oeiras, Portugal
    Mech Dev 124:884-97. 2007
    ..Embryos defective in Dpp signalling display defects in AS cell shape changes, specifically in the reduction of their apical surface areas, leading to defective AS contraction...
  69. ncbi Rab35 regulates neurite outgrowth and cell shape
    Julien Chevallier
    Department of Anatomy and Cell Biology, McGill University, Montreal, Quebec, Canada
    FEBS Lett 583:1096-101. 2009
    ..We conclude that one function of Rab35 is to regulate Rho-family GTPases and that this role has consequences for neurite outgrowth...
  70. pmc Scribble mutants promote aPKC and JNK-dependent epithelial neoplasia independently of Crumbs
    Gregory R Leong
    Peter MacCallum Cancer Centre, Victoria, Australia
    BMC Biol 7:62. 2009
    ..Increasing evidence also implicates the loss of human Scrib in cancer. In this report, we investigate how loss of Scrib promotes epithelial tumourigenesis in Drosophila, both alone and in cooperation with oncogenic mutations...
  71. pmc End-on microtubule-dynein interactions and pulling-based positioning of microtubule organizing centers
    Liedewij Laan
    Faculty of Arts and Sciences, Center for Systems Biology, Harvard University, Cambridge, MA, USA
    Cell Cycle 11:3750-7. 2012
    ..This effect is predicted to be strongly geometry-dependent, and we therefore finally discuss ongoing efforts to repeat these experiments in three-dimensional, spherical and deformable geometries...
  72. ncbi Nasal septum-derived multipotent progenitors: a potent source for stem cell-based regenerative medicine
    Abbas Shafiee
    Stem Cell Biology Department, Stem Cell Technology Research Center, Tehran, Iran
    Stem Cells Dev 20:2077-91. 2011
    ..In conclusion, NSP could be considered as an alternative autologous cell source that can bring them to the top of therapeutic applications...
  73. ncbi Establishment and characterization of a lactating bovine mammary epithelial cell model for the study of milk synthesis
    Ke Zhao
    Institute of Dairy Science, MOE Key Laboratory of Molecular Animal Nutrition, Zhejiang University, No 268 Kaixuan Road, Hangzhou 310029, Peoples Republic of China
    Cell Biol Int 34:717-21. 2010
    ..The transcription of the alphas1 casein gene and synthesis of alphas caseins were also detected in the model. Thus, our lactating BMEC model can be an effective model in vitro for studies of milk synthesis in the bovine mammary gland...
  74. pmc Automated characterization of cell shape changes during amoeboid motility by skeletonization
    Yuan Xiong
    Department of Electrical and Computer Engineering, Johns Hopkins University, Baltimore, MD 21218, USA
    BMC Syst Biol 4:33. 2010
  75. pmc Universal rule for the symmetric division of plant cells
    Sébastien Besson
    Department of Organismic and Evolutionary Biology, Harvard University, The Biological Laboratories, 16 Divinity Avenue, Cambridge, MA 02138, USA
    Proc Natl Acad Sci U S A 108:6294-9. 2011
    ..most recent algal ancestors confirms that the probability distribution is widely conserved and independent of cell shape and size...
  76. pmc Apical accumulation of Rho in the neural plate is important for neural plate cell shape change and neural tube formation
    Nagatoki Kinoshita
    Electron Microscope Laboratory and Organogenesis and Neurogenesis Group, RIKEN Center for Developmental Biology, Kobe 650 0047, Japan
    Mol Biol Cell 19:2289-99. 2008
    ..signaling pathway, the Wnt/PCP pathway, was implicated in the apical accumulation of Rho and regulation of cell shape in the neural plate, suggesting that this signal may be the spatiotemporal regulator of Rho in neural tube ..
  77. pmc Cell surface- and rho GTPase-based auxin signaling controls cellular interdigitation in Arabidopsis
    Tongda Xu
    Center for Plant Cell Biology, Department of Botany and Plant Sciences, University of California, Riverside, CA 92521, USA
    Cell 143:99-110. 2010
    ..These findings reveal Rho GTPase-based auxin-signaling mechanisms, which modulate the spatial coordination of cell expansion across a field of cells...
  78. ncbi Morphology, cytoskeletal organization, and myosin dynamics of mouse embryonic fibroblasts cultured on nanofibrillar surfaces
    Ijaz Ahmed
    Department of Pharmacology, UMDNJ Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA
    Mol Cell Biochem 301:241-9. 2007
    ..These results provide further evidence that nanofibrillar surfaces offer a significantly different environment for cells than 2D substrates...
  79. ncbi Subunits of the chaperonin CCT interact with F-actin and influence cell shape and cytoskeletal assembly
    Karen I Brackley
    Department of Cell and Molecular Biology, Göteborgs Universitet, Medicinaregatan 9C, 405 30 Goteborg, Sweden
    Exp Cell Res 316:543-53. 2010
    ..Reducing levels of individual CCT subunits implicates CCTepsilon in influencing cell shape and reduced levels of this subunit limit the cells' ability to recover from microfilament depolymerization...
  80. pmc Sculpting the bacterial cell
    William Margolin
    Department of Microbiology and Molecular Genetics, University of Texas Medical School at Houston, 6431 Fannin Street, Houston, TX 77030, USA
    Curr Biol 19:R812-22. 2009
    ..Other bacteria that lack MreB homologs or even cell walls use distinct cytoskeletal systems to maintain their distinct shapes. Here I review what is known about the mechanisms that determine the shape of prokaryotic cells...
  81. ncbi Quantitative morphological study of microglial cells in the ischemic rat brain using principal component analysis
    Z Soltys
    Department of Neuroanatomy, Institute of Zoology, Jagiellonian University, 6 Ingardena St, 30 060 Cracow, Poland
    J Neurosci Methods 146:50-60. 2005
    ..The results presented show that the quantitative morphological analysis can be an effective tool in research on the reactive behaviour of microglia and, particularly, in the detection of small and early changes in the cells...
  82. ncbi Willin and Par3 cooperatively regulate epithelial apical constriction through aPKC-mediated ROCK phosphorylation
    Takashi Ishiuchi
    RIKEN Center for Developmental Biology, Chuo Ku, Kobe 650 0047, Japan
    Nat Cell Biol 13:860-6. 2011
    ..Thus, we have uncovered a Willin/Par3-aPKC-ROCK pathway that controls epithelial apical morphology...
  83. pmc An SK3 channel/nWASP/Abi-1 complex is involved in early neurogenesis
    Stefan Liebau
    Institute for Anatomy and Cell Biology, Ulm University, Ulm, Germany
    PLoS ONE 6:e18148. 2011
    ..This observation indicates that the rearrangement of the actin based cytoskeleton via membrane bound SK3-channels might selectively be controlled in defined micro compartments of the cell...
  84. ncbi Phenotypic and functional characteristics of mesenchymal stem cells differentiated along a Schwann cell lineage
    Jenny Caddick
    Blond McIndoe Research Laboratories, University of Manchester, Manchester, United Kingdom
    Glia 54:840-9. 2006
    ..These findings support the assertion that MSCs can be differentiated into cells that are Schwann cell-like in terms of both phenotype and function...
  85. pmc Computational model for cell morphodynamics
    Danying Shao
    Center for Theoretical Biological Physics and Department of Physics, University of California, San Diego, La Jolla, California 92093 0374, USA
    Phys Rev Lett 105:108104. 2010
    ..Furthermore, we find that the dependence of the cell speed on this aspect ratio matches experimentally observed data...
  86. ncbi Biological characterization of long-term cultured human mesenchymal stem cells
    Jiseon Kim
    Pharmacological Research Department, National Institute of Toxicological Research, Korea Food and Drug Administration, Seoul, 122 704, Korea
    Arch Pharm Res 32:117-26. 2009
    ..These findings suggest that diverse tests for cellular therapy should be considered during the ex vivo culture of hMSCs, particularly when a prolonged and extended propagation period is required...
  87. pmc Control of cortical rigidity by the cytoskeleton: emerging roles for septins
    Julia Gilden
    Department of Pathology, University of California at San Francisco, San Francisco, California 94143 0511, USA
    Cytoskeleton (Hoboken) 67:477-86. 2010
    ..walls, this cortex governs the contact between their plasma membranes and the environment and thereby influences cell shape, motility, and signaling...
  88. ncbi Cullin mediates degradation of RhoA through evolutionarily conserved BTB adaptors to control actin cytoskeleton structure and cell movement
    Yuezhou Chen
    College of Life Science, Peking University, Beijing 100871, China
    Mol Cell 35:841-55. 2009
    ..Our studies reveal a previously unknown mechanism for controlling RhoA degradation and regulating RhoA function in various biological contexts, which involves a Cul3/BACURD ubiquitin ligase complex...
  89. pmc A dynamic stochastic model of frequency-dependent stress fiber alignment induced by cyclic stretch
    Hui Ju Hsu
    Department of Biomedical Engineering, Texas A and M University, College Station, Texas, United States of America
    PLoS ONE 4:e4853. 2009
    ..While there is much evidence that cells reorganize their SFs in response to matrix deformations, it is unclear how cells and their SFs determine their specific response to particular spatiotemporal changes in the matrix...
  90. ncbi Isolation and characterization of resident mesenchymal stem cells in human glomeruli
    Stefania Bruno
    Department of Internal Medicine, Molecular Biotechnology Center and Research Center for Experimental Medicine CeRMS, University of Torino, Torino, Italy and Fresenius Medical Care, Bad Homburg, Germany
    Stem Cells Dev 18:867-80. 2009
    ..In conclusion, these results indicate the presence of a multipotent mesenchymal cell population resident in human glomeruli that may have a role in the physiological cell turnover and/or in response to glomerular injury...
  91. ncbi Morphological and electrophysiological properties of GABAergic and non-GABAergic cells in the deep cerebellar nuclei
    Marylka Uusisaari
    Laboratory for Neuronal Circuit Dynamics, Brain Science Institute, RIKEN, 2 1 Hirosawa, Wako Shi, Saitama 351 0198, Japan
    J Neurophysiol 97:901-11. 2007
    ..We conclude that GABAergic DCN neurons are specialized to convey phasic spike rate information, whereas tonic spike rate is more faithfully relayed by the large non-GABAergic cells...
  92. ncbi Shroom family proteins regulate gamma-tubulin distribution and microtubule architecture during epithelial cell shape change
    Chanjae Lee
    Department of Molecular Cell and Developmental Biology, and Institute for Cellular and Molecular Biology, University of Texas, Austin, TX 78712, USA
    Development 134:1431-41. 2007
    b>Cell shape changes require the coordination of actin and microtubule cytoskeletons...
  93. pmc Difference in the cytotoxic effects of toxin B from Clostridium difficile strain VPI 10463 and toxin B from variant Clostridium difficile strain 1470
    Johannes Huelsenbeck
    Institut of Toxicology, Medical School, Hannover, D 30625 Hannover, Germany
    Infect Immun 75:801-9. 2007
    ..In conclusion, the protein substrate specificity of the glucosylating toxins determines their biological activity...
  94. ncbi Chemotaxis in shallow gradients is mediated independently of PtdIns 3-kinase by biased choices between random protrusions
    Natalie Andrew
    School of Biosciences, The University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK
    Nat Cell Biol 9:193-200. 2007
  95. ncbi Distinct cis-regulatory elements from the Dlx1/Dlx2 locus mark different progenitor cell populations in the ganglionic eminences and different subtypes of adult cortical interneurons
    Noel Ghanem
    Centre for Advanced Research in Environmental Genomics, Department of Biology, University of Ottawa, Ottawa, Ontario, Canada K1N 6N5
    J Neurosci 27:5012-22. 2007
    ..These data suggest that interneuron subtypes use distinct combinations of Dlx1/Dlx2 enhancers from the time they are specified through adulthood...
  96. pmc Tumor-derived endothelial cells exhibit aberrant Rho-mediated mechanosensing and abnormal angiogenesis in vitro
    Kaustabh Ghosh
    Vascular Biology Program, Departments of Pathology and Surgery, Children s Hospital and Harvard Medical School, Boston, MA 02115
    Proc Natl Acad Sci U S A 105:11305-10. 2008
  97. ncbi Centchroman induces G0/G1 arrest and caspase-dependent apoptosis involving mitochondrial membrane depolarization in MCF-7 and MDA MB-231 human breast cancer cells
    Manisha Nigam
    Tissue and Cell Culture Unit TCCU, Central Drug Research Institute, Lucknow, 226001, India
    Life Sci 82:577-90. 2008
    ..Results demonstrate that CC induces caspase-dependent apoptosis in MCF-7 and MDA MB-231 cells irrespective of ER status similar to TAM in terms of anti-neoplastic activity...
  98. pmc Mechanism of shape determination in motile cells
    Kinneret Keren
    Department of Biochemistry, Technion Israel Institute of Technology, Haifa 32000, Israel
    Nature 453:475-80. 2008
    ..treadmilling in an inextensible membrane bag can quantitatively recapitulate this spectrum and predict both cell shape and speed...
  99. ncbi Tmod3 regulates polarized epithelial cell morphology
    Kari L Weber
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    J Cell Sci 120:3625-32. 2007
    ..Here, we show that the pointed-end capping protein Tmod3 contributes to epithelial cell shape within confluent monolayers of polarized epithelial cells...
  100. ncbi Integrin-ECM interactions regulate the changes in cell shape driving the morphogenesis of the Drosophila wing epithelium
    Paloma Domínguez-Giménez
    Institut de Reserca Biomédica, Barcelona 08028, Spain
    J Cell Sci 120:1061-71. 2007
    ..wing epithelium to demonstrate that cell-ECM interactions mediated by integrins also regulate the changes in cell shape that underly epithelial morphogenesis...
  101. pmc Connecdenn 3/DENND1C binds actin linking Rab35 activation to the actin cytoskeleton
    Andrea L Marat
    Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, Canada
    Mol Biol Cell 23:163-75. 2012
    ..This interaction couples Rab35 activation to the actin cytoskeleton, resulting in dramatic changes in cell shape, notably the formation of protrusive membrane extensions...

Research Grants69

  1. 4th ASM Conference on Prokaryotic Cell Biology and Development
    Susan S Golden; Fiscal Year: 2012
    ..Because cell differentiation involves a reorganization of cell shape, division, metabolism and gene expression, the investigation of differentiating prokaryotic cells is providing ..
  2. Identifying Key Structural Elements in the Activation of the Arp2/3 Complex
    Patricia A Khuu; Fiscal Year: 2013
    ..The complex is essential for changes to cell shape associated with specific processes, such as endocytosis in yeast, and is required for the formation of actin-..
    NINA REDA SALAMA; Fiscal Year: 2010
    ..Using a visual screen of a transposon mutant library, we identified clones displaying two classes of altered cell shape: straight rod and curved vibrioid...
  4. Human Platelet Defects in Transcription Factor RUNX1 Haplodeficiency
    ANGARA KONETI RAO; Fiscal Year: 2013
    ..of proteins;and c) FLNA, TUBB1, related to cytoskeletal system, and play a major role in platelet production, cell shape, adhesion and secretion...
  5. Structure and function of stress fibers and cell adhesion sites
    Mary C Beckerle; Fiscal Year: 2013
    ..provided by applicant): The actin cytoskeleton is essential for cell viability and plays critical roles in cell shape, adhesion, and motility...
  6. Regulation of TGF-beta Signaling and Embryonic Development by GTPases
    Gerald H Thomsen; Fiscal Year: 2010
    ..by applicant): GTPases govern critical cellular processes such as signal transduction, transcription, mitosis, cell shape and cell movement by acting as molecular switches that regulate activity of protein partners...
  7. Role of the Tail Domain in Vinculin Function
    Sharon L Campbell; Fiscal Year: 2010
    ..PUBLIC HEALTH RELEVANCE Vinculin is an essential protein involved controlling cell shape and motility, in part by anchoring actin polymers to the membrane...
  8. Effect of Helicobacter pylori cell shape on pathogenesis
    ..Helical cell shape is thought to enhance H...
  9. Liver Tissue Engineering Through Three-Dimensional Hepatocyte Culture
    Tammy T Chang; Fiscal Year: 2013
    ..The research is driven by the hypotheses that 1) 3D cell shape and matrix rigidity are interconnected factors that together modulate hepatocyte function through cytoskeletal ..
  10. Membrane skeleton regulation of cell shape and interactions in lens development
    Velia M Fowler; Fiscal Year: 2013
    ..Mathias). These studies will establish basic mechanisms for gap junction control of lens transparency, and provide new insights for therapies in cataract prevention. ..
  11. Using shared mechanisms of morphogenesis to study neural tube defects
    DESCRIPTION (provided by applicant): Morphogenesis involves changes in cell shape regulated by complex networks that control cell polarity, cytoskeleton rearrangements and adhesive properties...
  12. Control of bacterial morphology and differentiation
    Yves V Brun; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Bacteria undergo specific cell shape changes and localize various proteins to subcellular sites, both of which are important for their survival in te environment...
  13. Cellular and Biochemical Studies of Myosin Assembly in Dictyostelium
    Thomas Egelhoff; Fiscal Year: 2009
    Myosin II plays fundamental roles in cytokinesis, cell migration, and cell shape changes during development...
  14. The biomechanics of morphogenesis in the frog
    Lance A Davidson; Fiscal Year: 2013
    ..analysis of the mechanics of convergent extension, identifying biomechanical mechanisms that regulate cell shape and drive mediolateral cell behaviors, establish passive tissue properties such as stiffness as well as active ..
  15. Design and Implementation of Genetically Encoded Myosin Based Force Sensors
    DANIEL PETER KIEHART; Fiscal Year: 2013
    ..Nevertheless, how such proteins collaborate with the dynamic cytoskeleton in vivo to produce forces that drive cell shape changes for morphogenesis, cytokinesis and cell locomotion is unclear...
  16. Quantitative Analysis of Chemotactic Motility Cycle of Ameboid Cells
    JUAN CARLOS LASHERAS; Fiscal Year: 2013
    ..We and others have observed that a considerable portion of the changes in cell shape occurring during amoeboid migration are due to periodic repetitive events, which enables the use of conditional ..
  17. Role of Par1 Polarity Proteins in Podocyte Development and Glomerular Disease
    KIMBERLY JEAN REIDY; Fiscal Year: 2013
    ..Dominant negative suppression of Par1a/b function in cultured podocytes induced changes of cell shape and altered expression of slit diaphragm proteins, which are key podocyte components that support glomerular ..
  18. Molecular Function of Palladin's Ig Domains in Cell Adhesion and Motility
    Carol A Otey; Fiscal Year: 2010
    ..Public Health Relevance: Actin filaments form complex subcellular arrays that are essential for establishing cell shape, maintaining adhesion between neighboring cells, and generating connections between cells and their underlying ..
  19. Embryonic Development of the Mammalian Epididymis
    Barry T Hinton; Fiscal Year: 2013
    ..outlined in the following three specific aims: (1) To test the hypothesis that Ptk7 plays an important role in cell shape and cell positioning, which in turn allow the Wolffian duct to undergo the morphogenic events of elongation and ..
  20. The Role of HMGB1 in Acute Lung Injury-Induced Endothelial Cell Permeability
    Rachel K Wolfson; Fiscal Year: 2012
    ..between HMGB1 and heat shock protein (Hsp) 27, which stabilizes the actin cytoskeleton in EC to maintain cell shape, using mutant forms of Hsp27 to study the role of Hsp27 phosphorylation in HMGB1 - mediated EC barrier ..
  21. Peptidoglycan Biogenesis in Escherichia Coli
    Thomas G Bernhardt; Fiscal Year: 2013
    ..with a crosslinked polysaccharide polymer called peptidoglycan (PG) that is critical for the maintenance of cell shape and integrity...
  22. Directing Stem Cell Differentiation on Biologically Inspired Micropatterned Surfa
    Anita Shukla; Fiscal Year: 2012
    ..stiffness, initial studies in which cells were cultured on protein patterns of arbitrary shape show that cell shape also has a strong effect on hMSC differentiation...
  23. Mechanism of DLC1-mediated tumor suppression
    Su Hao Lo; Fiscal Year: 2013
    ..Investigation of the molecular mechanism how DLC1 regulates cell shape, adhesion, migration, and tumorigenicity Specific Aim 2...
  24. Regulation of Rho family GTPases by heterotrimeric G proteins
    BARRY S KREUTZ; Fiscal Year: 2013
    ..to be important for various important cellular processes including cancer progression, cell transformation, cell shape changes, or cell growth and differentiation...
  25. Regulation and Function of a Bacterial Cytoskeleton
    R Dyche Mullins; Fiscal Year: 2013
    ..has identified a number of cytoskeletal systems in bacteria, including actin-like proteins required to maintain cell shape (Jones, 2001);transport cargo through cytoplasm (Moller-Jensen, 2003;Kruse, 2003);and organize intracellular ..
  26. Peptidoglycan synthesis during sporulation
    Jonathan Dworkin; Fiscal Year: 2012
    ..Mutations in these genes lead to defects in cell shape consistent with a proposed role in cell wall synthesis...
  27. Microtubule Stability in Lung Endothelium
    CRISTHIAAN D OCHOA ARENAS; Fiscal Year: 2012
    ..Despite their importance in control of endothelial cell shape, relatively little is known about intracellular signals that control microtubule assembly and disassembly...
  28. Signaling pathways that mediate mammalian oocyte cortical mechanics
    Douglas N Robinson; Fiscal Year: 2013
    ..oocyte mechanics, this project seeks to analyze the functions of key proteins in cellular mechanics and cell shape regulation during progression of the mammalian oocyte through meiosis and cytokinesis...
  29. Cytoskeletal Regulation of Erythrocyte Glucose Transporter-1
    Athar H Chishti; Fiscal Year: 2012
    SUMMARY Erythrocyte spectrin-actin junctions play a critical role in the maintenance of cell shape and membrane properties. Their organization and membrane linkage is therefore of fundamental importance to erythrocyte physiology...
  30. Biophysical Mechanisms of Drosophila Development.
    Jennifer A Zallen; Fiscal Year: 2013
    ..of multicellular organisms are established during development by spatially and temporally regulated changes in cell shape and behavior...
  31. Supramolecular Complexes That Mediate Pneumococcal PG Biosynthesis and Virulence
    Malcolm E Winkler; Fiscal Year: 2012
    ..PG forms the major rigid structure in the cell wall that determines cell shape and size and serves as the scaffolding onto which other pneumococcal virulence factors are covalently attached, ..
  32. Architecture of a Genetic Network Governing Morphogenesis
    David H A Fitch; Fiscal Year: 2013
    ..downstream of DMD-3 and an understanding of how it governs the localization or expression of key effectors of cell shape change...
  33. Rac proteins in hematopoietic cell survival and function
    David A Williams; Fiscal Year: 2013
    ..accomplished by specific functional modules of Vav and Pak proteins that distinctly regulate kinase activation, cell shape changes, and gene expression...
  34. Regulation of call adhesion in Xenopus
    KEN CHO; Fiscal Year: 2009
    ..to be involved in mesoderm patterning such as Wnt and activin/nodal also influence mesodermal morphogenesis and cell shape. The focus of this project is to uncover the molecular function of FLRT3 (Fibronectin Leucine-rich Repeat ..
  35. TGF-beta-induced non-Smad signaling events and cancer cell behavior
    RIK M DERYNCK; Fiscal Year: 2013
    ..These may explain non-transcription responses to TGF-[unreadable] such as migration, changes in cell shape and protein translation, yet may also affect the activities of the Smads...
    Nadean L Brown; Fiscal Year: 2012
    ..In particular, Notch signaling regulates proliferation, apoptosis, cell shape changes, differentiation and stem cell maintenance...
  37. Cytoskeleton polymerization dynamics in the cell cycle
    Timothy J Mitchison; Fiscal Year: 2010
    ..b>Cell shape is governed by the cytoskeleton, a system of fibers in the cytoplasm made of the subunit proteins actin and ..
  38. Systematic Phenotypic Analysis of the Gram-positive Envelope
    Jason M Peters; Fiscal Year: 2013
    ..subtilis deletion library to determine the function of ylaN, a gene involved in cell shape. These high-throughput methodologies will accelerate the assignment of phenotypes and functions to the large ..
  39. Brain Pericyte and Amyloid-beta Peptide Interaction
    Donghui Zhu; Fiscal Year: 2013
    ..Given that cytoskeletal and contratile proteins play key roles in cell shape, contraction, mobility and BBB permeability regulation, then our investigation of these proteins'expression,..
  40. Function of Desmoglein 1/Pemphigus Foliaceus Antigen
    KATHLEEN JANEE GREEN; Fiscal Year: 2013
    ..and its associated protein Erbin regulate RhoGTPase-dependent signaling to promote actin remodeling, control cell shape and regulate serum response factor (SRF)-mediated transcriptional programs necessary for epidermal ..
    Deborah J Andrew; Fiscal Year: 2013
    ..forms using the same morphogenetic changes as more complicated organs of higher animals, including changes in cell shape, adhesion and movement...
  42. Reducing genetic buffering to identify QTLs that affect cell shape and growth
    Kerry A Geiler-Samerotte; Fiscal Year: 2013
    ..Identifying QTLs contributing to cell morphology and growth is an important goal in understanding the genetic basis of diseases, cancers in particular. ..
  43. Analysis of the Borrelia burgdorferi Integrin Ligand P66
    Jenifer L Coburn; Fiscal Year: 2013
    ..are consistent with the hypothesis that host responses to pathogens, such as phagocytosis and maintenance of cell shape and tissue integrity, are affected by P66...
  44. PI(3,4,5)P3 Regulates Cell Polarity During Chemotaxis and Cytokinesis
    Christopher Janetopoulos; Fiscal Year: 2012
    ..We are using the amoeba Dictyostelium as a model system to understand the basic mechanisms that control these changes in cell shape. Many of the homologous components we are studying are mutated or lost in many human diseases.
  45. Molecular Mechanisms of Organelle Assembly by the Prokaryotic Actin Homolog MamK
    Arash Komeili; Fiscal Year: 2013
    ..These cytoskeletal proteins are essential for cell division, cell shape maintenance and chromosome segregation in a variety of prokaryotic species and hold promise as targets for the ..
  46. Investigating the molecular and mechanical regulation of pulsed actomyosin contra
    ADAM CHRISTOPHER MARTIN; Fiscal Year: 2013
    ..Morphogenesis is driven by forces generated by individual cells, which result in changes in cell shape and tissue mechanics...
  47. Regulation of Lung Cancer Metastasis by miR-200
    Jonathan M Kurie; Fiscal Year: 2013
    ..on adjacent tumor cells;Notch then suppresses the levels of a small RNA called miR-200, inducing a change in cell shape and detachment from neighboring cells, an early event in metastasis...
  48. Fiber Cell Cytoskeletal Scaffolds-Role in Lens Architecture and Function
    P Vasantha Rao; Fiscal Year: 2011
    ..project are to identify and characterize the membrane cytoskeletal scaffolding proteins regulating lens fiber cell shape, cell-cell interactions, membrane stability and organization, and to determine how dysregulation of this ..
  49. Microtubule Complexes Involved in Intracelluar Transport
    Ronald D Vale; Fiscal Year: 2013
    The microtubule cytoskeleton plays an essential role in cell shape, migration, and division...
  50. Cell adhesion, signal transduction and cytoskeletal regulation in Drosophila
    Mark Peifer; Fiscal Year: 2013
    ..work together and separately to regulate establishment and maintenance of apical-basal polarity and columnar cell shape. Our data reveal that Canoe and Rap1 are critical regulators of AJ:actin linkage during morphogenesis, and ..
  51. Par1-substrates responsible for CagA-mediated pathogenesis of Helicobacter pylori
    Markus Stein; Fiscal Year: 2013
    ..Our goal is to evaluate the contribution of all validated substrates to CagA-mediated loss of epithelial cell shape and polarity...
  52. Elucidation of a bacterial cell shape generating program and pathogenic functions
    NINA REDA SALAMA; Fiscal Year: 2013
    ..Most descriptions of H. pylori virulence include the widely-accepted hypothesis that its helical cell shape enhances colonization of the stomach...
  53. Developmental Regulation of Collective Cell Migration
    Denise J Montell; Fiscal Year: 2013
    ..is significant because Rho, Rac and Cdc42 are key nodes in the signaling and cytoskeletal networks that control cell shape and movement...
    Sanjoy K Bhattacharya; Fiscal Year: 2013
    ..which TM cells sense the fluid flow fluctuations in the ECM and by which the sensation is transduced to change cell shape and motility to increase or decrease TM pore size, thus regulating the passing of fluid through TM...
  55. Bacterial cell wall synthesis, shape and septation
    Kevin D Young; Fiscal Year: 2013
    ..Yet only in the last 5-7 years has it become clear that these differences are much more than cosmetic. Instead, cell shape is of fundamental and medical importance, contributing to bacterial survival and virulence by influencing ..
  56. Molecular motors in cell biology
    Yale E Goldman; Fiscal Year: 2013
    ..their actions with dynamic (and highly regulated) cytoskeletal filaments to control cell growth, define cell shape, deliver and polarize intracellular cargoes, traffic endosomal membranes, and participate in signaling cascades...
  57. Intercellular adhesion and morphogenesis: role of actin-regulatory proteins
    Ann Marie Pendergast; Fiscal Year: 2010
    DESCRIPTION (provided by applicant): Coordinate regulation of cell shape changes and intercellular adhesion is critical for morphogenesis, cell migration, survival and growth during development...
  58. Cell-Cell Signaling During Mammalian Early Eye Formation
    Nadean L Brown; Fiscal Year: 2011
    ..In particular, Notch signaling regulates proliferation, cell shape changes, differentiation and stem cell maintenance...
  59. In-Incubator Live Cell Fluorescence/DIC Time Lapse Imaging System
    Martin F Schneider; Fiscal Year: 2010
    ..This capability is ideal for monitoring changes in cell shape, intracellular structure, and intracellular distribution of fusion proteins, and intracellular distribution of ..
  60. Characterization of the SUMO ligase PIAS1 in tumor progression and metastasis
    JERFIZ D CONSTANZO; Fiscal Year: 2013
    ..by potentiating tumor survival during cellular stress and in the promotion of cell migration by modulating cell shape, potentially through RAC/RHO and FAK proteins...
  61. The Structural Basis of Shroom-Mediated Cell Contractility
    ANDREW PAUL VANDEMARK; Fiscal Year: 2013
    ..The actin-associated Shroom proteins are critical determinants of epithelial cell shape, controlling the process of apical constriction and cellular contractility...
  62. High resolution electron cryo-microscope at Brandeis University.
    Nikolaus Grigorieff; Fiscal Year: 2009
    ..Cytoplasmic dynein plays a major role in cell division, signaling, cell shape, and polarized cell growth...
  63. Blood Brain Barrier Physiology and HIV Dementia
    HOWARD GENDELMAN; Fiscal Year: 2007
    ..was paid to the spatial parameters of cell biophysiology, migration and the mechanisms underlying changes in cell shape and volume...
  64. DNA-crosslinked gels: New advanced biomaterials
    NOSHIR LANGRANA; Fiscal Year: 2006
    ..and, thus, represent a novel class of biomimatic materials that may shed light on the mechanical basis of cell shape stability...
  65. Investigating the molecular and mechanical regulation of pulsed actomyosin contra
    ADAM CHRISTOPHER MARTIN; Fiscal Year: 2010
    ..Morphogenesis is driven by forces generated by individual cells, which result in changes in cell shape and tissue mechanics...
    RONALD LIMBERGER; Fiscal Year: 2002
    ..The periplasmic location of the flagellar filament, together with the cell shape, enables the spirochete to move through dense matrices that would inhibit most other bacteria and assists in ..
  67. Intermediate filament cytoskeleton and cell shape in Caulobacter crescentus
    Christine Jacobs Wagner; Fiscal Year: 2009
    ..Knowing the basis for intermediate filament function may reveal how intermediate filament-based diseases develop, suggesting treatment strategies. ..
  68. Cell Shape Control of Migration on Biomaterials
    Chia Chi Ho; Fiscal Year: 2006
    ..A successful proof-of-principle demonstration of how cell shape, defined by the geometry of micropatterns, can be used to control the directional migration of cells on ..
  69. Thrombin signaling and HIV dementia
    Katherine Conant; Fiscal Year: 2006
    ..Activation of endothelial cell PARs has been linked to changes in cell shape, increased chemokine release, and increased adhesion molecule expression...