mutagenesis

Summary

Summary: Process of generating a genetic MUTATION. It may occur spontaneously or be induced by MUTAGENS.

Top Publications

  1. ncbi Genes required for mycobacterial growth defined by high density mutagenesis
    Christopher M Sassetti
    Department of Immunology and Infectious Diseases, Harvard School of Public Health, Harvard Medical School, Boston, MA 02115, USA
    Mol Microbiol 48:77-84. 2003
  2. ncbi FACS-optimized mutants of the green fluorescent protein (GFP)
    B P Cormack
    Department of Microbiology and Immunology, Stanford University School of Medicine, CA 94305 5402, USA
    Gene 173:33-8. 1996
  3. pmc Rapid "open-source" engineering of customized zinc-finger nucleases for highly efficient gene modification
    Morgan L Maeder
    Molecular Pathology Unit and Center for Cancer Research, Massachusetts General Hospital, Charlestown, MA 02129, USA
    Mol Cell 31:294-301. 2008
  4. pmc Natural mutagenesis of human genomes by endogenous retrotransposons
    Rebecca C Iskow
    Genetics and Molecular Biology Graduate Program, Emory University, Atlanta, GA 30322, USA
    Cell 141:1253-61. 2010
  5. pmc Improved somatic mutagenesis in zebrafish using transcription activator-like effector nucleases (TALENs)
    Finola E Moore
    Molecular Pathology Unit and Center for Cancer Research, Massachusetts General Hospital, Charlestown, Massachusetts, United States of America
    PLoS ONE 7:e37877. 2012
  6. pmc APOBEC3G contributes to HIV-1 variation through sublethal mutagenesis
    Holly A Sadler
    Institute for Molecular Virology, 18 242 Moos Tower, 515 Delaware St SE, University of Minnesota, Minneapolis, MN 55455, USA
    J Virol 84:7396-404. 2010
  7. ncbi Efficient transposition of the piggyBac (PB) transposon in mammalian cells and mice
    Sheng Ding
    Institute of Developmental Biology and Molecular Medicine, School of Life Sciences, Fudan University, 220 Handan Road, Shanghai 200433, China
    Cell 122:473-83. 2005
  8. ncbi Initial sequencing and comparative analysis of the mouse genome
    Robert H Waterston
    Genome Sequencing Center, Washington University School of Medicine, Campus Box 8501, 4444 Forest Park Avenue, St Louis, Missouri 63108, USA
    Nature 420:520-62. 2002
  9. doi Increased mutagenesis and unique mutation signature associated with mitotic gene conversion
    Wade M Hicks
    Department of Biology and Rosenstiel Center, Brandeis University, Waltham, MA 02454 9110, USA
    Science 329:82-5. 2010
  10. pmc Retroviral elements and their hosts: insertional mutagenesis in the mouse germ line
    Irina A Maksakova
    Terry Fox Laboratory, BC Cancer Agency, Vancouver, British Columbia, Canada
    PLoS Genet 2:e2. 2006

Detail Information

Publications315 found, 100 shown here

  1. ncbi Genes required for mycobacterial growth defined by high density mutagenesis
    Christopher M Sassetti
    Department of Immunology and Infectious Diseases, Harvard School of Public Health, Harvard Medical School, Boston, MA 02115, USA
    Mol Microbiol 48:77-84. 2003
    ....
  2. ncbi FACS-optimized mutants of the green fluorescent protein (GFP)
    B P Cormack
    Department of Microbiology and Immunology, Stanford University School of Medicine, CA 94305 5402, USA
    Gene 173:33-8. 1996
    ..coli, the folding of the mutant proteins is more efficient than folding of wt GFP. These two properties contribute to a greatly increased (100-fold) fluorescence intensity, making the mutants useful for a number of applications...
  3. pmc Rapid "open-source" engineering of customized zinc-finger nucleases for highly efficient gene modification
    Morgan L Maeder
    Molecular Pathology Unit and Center for Cancer Research, Massachusetts General Hospital, Charlestown, MA 02129, USA
    Mol Cell 31:294-301. 2008
    ..In summary, OPEN provides an "open-source" method for rapidly engineering highly active zinc-finger arrays, thereby enabling broader practice, development, and application of ZFN technology for biological research and gene therapy...
  4. pmc Natural mutagenesis of human genomes by endogenous retrotransposons
    Rebecca C Iskow
    Genetics and Molecular Biology Graduate Program, Emory University, Atlanta, GA 30322, USA
    Cell 141:1253-61. 2010
    ..technologies are not capable of detecting most of these young insertions, and the true extent of germline mutagenesis by endogenous human retrotransposons has been difficult to examine...
  5. pmc Improved somatic mutagenesis in zebrafish using transcription activator-like effector nucleases (TALENs)
    Finola E Moore
    Molecular Pathology Unit and Center for Cancer Research, Massachusetts General Hospital, Charlestown, Massachusetts, United States of America
    PLoS ONE 7:e37877. 2012
    ..induced DNA mutations at genomic target sites while only a subset of CoDA ZFNs exhibited detectable rates of mutagenesis. TALENs also exhibited higher mutation rates than CoDA ZFNs that had not been pre-screened using a bacterial ..
  6. pmc APOBEC3G contributes to HIV-1 variation through sublethal mutagenesis
    Holly A Sadler
    Institute for Molecular Virology, 18 242 Moos Tower, 515 Delaware St SE, University of Minnesota, Minneapolis, MN 55455, USA
    J Virol 84:7396-404. 2010
    ..cytosine deaminase activity that attacks the nascent viral cDNA during reverse transcription, causing lethal mutagenesis. It has been suggested, but not formally shown, that APOBEC3G can also induce sublethal mutagenesis, which ..
  7. ncbi Efficient transposition of the piggyBac (PB) transposon in mammalian cells and mice
    Sheng Ding
    Institute of Developmental Biology and Molecular Medicine, School of Life Sciences, Fudan University, 220 Handan Road, Shanghai 200433, China
    Cell 122:473-83. 2005
    ..used for genetic manipulation in lower organisms, including generating transgenic animals and insertional mutagenesis. In contrast, the usage of transposons in mice and other vertebrate systems is still limited due to the lack of ..
  8. ncbi Initial sequencing and comparative analysis of the mouse genome
    Robert H Waterston
    Genome Sequencing Center, Washington University School of Medicine, Campus Box 8501, 4444 Forest Park Avenue, St Louis, Missouri 63108, USA
    Nature 420:520-62. 2002
    ....
  9. doi Increased mutagenesis and unique mutation signature associated with mitotic gene conversion
    Wade M Hicks
    Department of Biology and Rosenstiel Center, Brandeis University, Waltham, MA 02454 9110, USA
    Science 329:82-5. 2010
    ..These observations suggest that increased DSB frequencies in oncogene-activated mammalian cells may also increase the probability of acquiring mutations required for transition to a cancerous state...
  10. pmc Retroviral elements and their hosts: insertional mutagenesis in the mouse germ line
    Irina A Maksakova
    Terry Fox Laboratory, BC Cancer Agency, Vancouver, British Columbia, Canada
    PLoS Genet 2:e2. 2006
    ..The prevalence of mouse ERV activity indicates that the genome of the laboratory mouse is presently behind in the "arms race" against invasion...
  11. doi En passant mutagenesis: a two step markerless red recombination system
    B Karsten Tischer
    Institut fur Virologie, Freie Universitat Berlin, Berlin, Germany
    Methods Mol Biol 634:421-30. 2010
    ..In addition to RecA-based shuttle mutagenesis, Red recombination is commonly used for sequence modification...
  12. ncbi A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications
    Takeharu Nagai
    Laboratory for Cell Function and Dynamics, Advanced Technology Development Center, Brain Science Institute, RIKEN, 2 1 Hirosawa, Wako City, Saitama, 351 0198, Japan
    Nat Biotechnol 20:87-90. 2002
    ..Over the last several years, mutagenesis studies have improved folding properties of GFP (refs 1,2)...
  13. pmc High efficiency mutagenesis, repair, and engineering of chromosomal DNA using single-stranded oligonucleotides
    H M Ellis
    Gene Regulation and Chromosome Biology Laboratory, Division of Basic Sciences, National Cancer Institute at Frederick, P.O. Box B, Frederick, MD 21702, USA
    Proc Natl Acad Sci U S A 98:6742-6. 2001
    ..Beta protein and ssDNA may prove generally applicable for repairing DNA in many organisms...
  14. pmc Human quiescin-sulfhydryl oxidase, QSOX1: probing internal redox steps by mutagenesis
    Erin J Heckler
    Department of Chemistry and Biochemistry, University of Delaware, Newark, Delaware 19716, USA
    Biochemistry 47:4955-63. 2008
    ..The four other cysteine residues chosen for mutagenesis, C70, C73, C449, and C452, are all crucial for efficient oxidation of reduced RNase...
  15. pmc Discovery of rare mutations in populations: TILLING by sequencing
    Helen Tsai
    Department of Plant Biology and Genome Center, University of California, Davis, California 95616, USA
    Plant Physiol 156:1257-68. 2011
    ..Discovery was robust both in rice and wheat using either bidimensional or tridimensional pooling schemes. The method compared favorably with other molecular and computational approaches, providing high sensitivity and specificity...
  16. ncbi ENU mutagenesis, a way forward to understand gene function
    Abraham Acevedo-Arozena
    MRC Mammalian Genetics Unit, Harwell, Oxfordshire, OX11 0RD, United Kingdom
    Annu Rev Genomics Hum Genet 9:49-69. 2008
    ..N-ethyl-N-nitrosourea (ENU) mutagenesis has been widely used for both approaches to generate mouse mutants...
  17. pmc Mutagenesis and mapping of a mouse gene, Clock, essential for circadian behavior
    M H Vitaterna
    Department of Neurobiology and Physiology, Northwestern University, Evanston, IL 60208
    Science 264:719-25. 1994
    ..The power of ENU mutagenesis combined with the ability to clone murine genes by map position provides a generally applicable approach to ..
  18. pmc Estimating the per-base-pair mutation rate in the yeast Saccharomyces cerevisiae
    Gregory I Lang
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    Genetics 178:67-82. 2008
    ..44x10(-10) at CAN1) and we propose a definition for the effective target size of genes (the probability that a mutation inactivates the gene) that acknowledges that the mutation rate is nonuniform across the genome...
  19. ncbi The ClosTron: Mutagenesis in Clostridium refined and streamlined
    John T Heap
    BBSRC Sustainable BioEnergy Centre, School of Molecular Medical Sciences, Centre for Biomolecular Sciences, The University of Nottingham, University Park, Nottingham, NG7 2RD, UK
    J Microbiol Methods 80:49-55. 2010
    The recent development of the ClosTron Group II intron directed mutagenesis tool for Clostridium has advanced genetics in this genus, and here we present several significant improvements...
  20. ncbi A genetic screen for mutations affecting embryogenesis in zebrafish
    W Driever
    Cardiovascular Research Center, Massachusetts General Hospital, Charlestown 02129, USA
    Development 123:37-46. 1996
    Systematic genome-wide mutagenesis screens for embryonic phenotypes have been instrumental in the understanding of invertebrate and plant development...
  21. ncbi The mechanisms of UV mutagenesis
    Hironobu Ikehata
    Division of Genome and Radiation Biology, Department of Cell Biology, Graduate School of Medicine, Tohoku University, Sendai, Japan
    J Radiat Res 52:115-25. 2011
    ..In contrast, it has been demonstrated that UVA exclusively induces the solar-UV signature mutations in vivo through CPD formation...
  22. pmc Genome instability due to ribonucleotide incorporation into DNA
    Stephanie A Nick McElhinny
    Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, North Carolina, USA
    Nat Chem Biol 6:774-81. 2010
    ....
  23. pmc Genetic steps of mammalian homologous repair with distinct mutagenic consequences
    Jeremy M Stark
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, 1275 York Ave, New York, NY 10021, USA
    Mol Cell Biol 24:9305-16. 2004
    ..These results imply that the proper genetic interplay of repair factors is essential to limit the mutagenic potential of DSB repair...
  24. ncbi Phenotypic characterization of a new Grin1 mutant mouse generated by ENU mutagenesis
    Tamio Furuse
    Technology and Development Team for Mouse Phenotype Analysis Japan Mouse Clinic, RIKEN BioResource Center, 3 1 1 Koyadai, Tsukuba, Ibaraki 305 0074, Japan
    Eur J Neurosci 31:1281-91. 2010
    In the RIKEN large-scale N-ethyl-N-nitrosourea (ENU) mutagenesis project we screened mice with a dominant mutation that exhibited abnormal behavior in the open-field test, passive avoidance test and home-cage activity test...
  25. pmc PCNA ubiquitination is important, but not essential for translesion DNA synthesis in mammalian cells
    Ayal Hendel
    Department of Biological Chemistry, Weizmann Institute of Science, Rehovot, Israel
    PLoS Genet 7:e1002262. 2011
    ..specialized low-fidelity DNA polymerases bypass replication-blocking lesions, and it is usually associated with mutagenesis. In Saccharomyces cerevisiae a key event in TLS is the monoubiquitination of PCNA, which enables recruitment of ..
  26. pmc Massively parallel sequencing of the mouse exome to accurately identify rare, induced mutations: an immediate source for thousands of new mouse models
    T D Andrews
    Immunogenomics Laboratory, Australian National University, GPO Box 334, Canberra City, Australian Capital Territory, 2601, Australia
    Open Biol 2:120061. 2012
    ..This approach transforms genetic screens in mice, establishes a general strategy for analysing rare DNA variants and opens up a large new source for experimental models of human disease...
  27. pmc Mutagenic processing of ribonucleotides in DNA by yeast topoisomerase I
    Nayun Kim
    Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC 27710, USA
    Science 332:1561-4. 2011
    ..The reported studies extend the role of Top1 to include the processing of rNMPs in genomic DNA into irreversible single-strand breaks, an activity that can have distinct mutagenic consequences and may be relevant to human disease...
  28. pmc Genome-wide mutagenesis of Xanthomonas axonopodis pv. citri reveals novel genetic determinants and regulation mechanisms of biofilm formation
    Jinyun Li
    Department of Microbiology and Cell Science, Citrus Research and Education Center, University of Florida, Lake Alfred, Florida, United States of America
    PLoS ONE 6:e21804. 2011
    ..The report provides significant new information about the genetic determinants and regulatory mechanism of biofilm formation...
  29. ncbi Placental defect and embryonic lethality in mice lacking hepatocyte growth factor/scatter factor
    Y Uehara
    Institute for Liver Research, Kansai Medical University, Moriguchi, Osaka, Japan
    Nature 373:702-5. 1995
    ..These findings suggest that HGF/SF is an essential mediator of allantoic mesenchyme-trophoblastic epithelia interaction required for placental organogenesis...
  30. doi Mouse phenotyping
    Helmut Fuchs
    Institute of Experimental Genetics, Helmholtz Zentrum Munchen, German Research Center for Environmental Health, Ingolstädter Landstraße 1, 85764 München Neuherberg, Germany
    Methods 53:120-35. 2011
    ..Within the last 20 years many mutant mouse lines have been generated by different methods such as ENU mutagenesis, constitutive and conditional knock-out approaches, knock-down, introduction of human genes, and knock-in ..
  31. pmc Insertional mutagenesis combined with acquired somatic mutations causes leukemogenesis following gene therapy of SCID-X1 patients
    Steven J Howe
    Centre for Immunodeficiency, Molecular Immunology Unit, UCL Institute of Child Health, University College London, London, United Kingdom
    J Clin Invest 118:3143-50. 2008
    ..Here, we describe the occurrence of clonal T cell acute lymphoblastic leukemia (T-ALL) promoted by insertional mutagenesis in a completed gene therapy trial of 10 SCID-X1 patients...
  32. pmc Chromosomal transposition of PiggyBac in mouse embryonic stem cells
    Wei Wang
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire CB10 1SA, United Kingdom
    Proc Natl Acad Sci U S A 105:9290-5. 2008
    ..The comprehensive information from this study should facilitate further exploration of the potential of PB and SB DNA transposons in mammalian genetics...
  33. ncbi Exportin 1 (Crm1p) is an essential nuclear export factor
    K Stade
    Department of Biochemistry and Biophysics, University of California at San Francisco, 94143 0414, USA
    Cell 90:1041-50. 1997
    ..cerevisiae. The presented data are consistent with the conclusion that Crm1p is a carrier for the NES-mediated protein export pathway. We propose CRM1 be renamed exportin 1 (XPO1)...
  34. ncbi Fractionation mutagenesis and similar consequences of mechanisms removing dispensable or less-expressed DNA in plants
    Michael Freeling
    Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720, USA
    Curr Opin Plant Biol 15:131-9. 2012
    ..Regulatory DNA deletion following polyploidy combined with abundant RNA-seq expression datasets are being used to generate testable hypothesizes regarding the function of specific cis-regulatory sequences...
  35. pmc Vibrio cholerae triggers SOS and mutagenesis in response to a wide range of antibiotics: a route towards multiresistance
    Zeynep Baharoglu
    Institut Pasteur, Unité Plasticité du Génome Bactérien, Département Génomes et Génétique, 25 rue du Dr Roux, F 75015 Paris, France
    Antimicrob Agents Chemother 55:2438-41. 2011
    ..We show that unlike the situation with E. coli, all these antibiotics induce SOS in V. cholerae...
  36. pmc DNA polymerase δ and ζ switch by sharing accessory subunits of DNA polymerase δ
    Andrey G Baranovskiy
    Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, Nebraska 68198, USA
    J Biol Chem 287:17281-7. 2012
    ..Amino acid changes in Pol ζ that prevent the assembly of the [4Fe-4S] cluster abrogate Pol ζ function in UV mutagenesis. On the basis of these data, we propose that Pol switches at replication-blocking lesions occur by the exchange ..
  37. pmc Concomitant lethal mutagenesis of human immunodeficiency virus type 1
    Michael J Dapp
    Institute for Molecular Virology, Academic Health Center, University of Minnesota, Minneapolis, MN 55455, USA
    J Mol Biol 419:158-70. 2012
    ..One such approach, termed lethal mutagenesis, is directed at targeting the virus population structure for extinction or error catastrophe...
  38. pmc Suppressor mutagenesis identifies a velvet complex remediator of Aspergillus nidulans secondary metabolism
    Mona I Shaaban
    Department of Medical Microbiology and Immunology, University of Wisconsin Madison, Madison, WI 53706, USA
    Eukaryot Cell 9:1816-24. 2010
    ..An intact Velvet complex coupled with an OE::rsmA allele increases SM many fold over the wild-type level, but loss of rsmA does not decrease SM. RsmA encodes a putative bZIP basic leucine zipper-type transcription factor...
  39. pmc A chemosensory system that regulates biofilm formation through modulation of cyclic diguanylate levels
    Jason W Hickman
    Department of Microbiology, Box 357242, University of Washington, 1959 Northeast Pacific Street, Seattle, WA 98195, USA
    Proc Natl Acad Sci U S A 102:14422-7. 2005
    ..Together, the data suggest that the wsp signal transduction pathway regulates biofilm formation through modulation of cyclic diguanylate levels...
  40. ncbi Knowledge-based computational mutagenesis for predicting the disease potential of human non-synonymous single nucleotide polymorphisms
    Majid Masso
    Laboratory for Structural Bioinformatics, Department of Bioinformatics and Computational Biology, George Mason University, 10900 University Blvd MS 5B3, Manassas, VA 20110, USA
    J Theor Biol 266:560-8. 2010
    ..by quantifying environmental perturbations in the associated proteins with the use of a computational mutagenesis methodology that relies on a four-body, knowledge-based, statistical contact potential...
  41. ncbi A switch from high-fidelity to error-prone DNA double-strand break repair underlies stress-induced mutation
    Rebecca G Ponder
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA
    Mol Cell 19:791-804. 2005
    ..We provide evidence that the molecular basis for stress-induced mutagenesis in an E. coli model is error-prone DNA double-strand break repair (DSBR)...
  42. ncbi Interactions in the error-prone postreplication repair proteins hREV1, hREV3, and hREV7
    Y Murakumo
    Department of Pathology, Nagoya University Graduate School of Medicine, 65 Tsurumai Cho, Showa Ku, Nagoya 466 8550, Japan
    J Biol Chem 276:35644-51. 2001
    ..hREV1) and REV3 (hREV3) genes were identified, and their products were revealed to be involved in UV-induced mutagenesis, as observed for their yeast counterparts...
  43. pmc Sublethal antibiotic treatment leads to multidrug resistance via radical-induced mutagenesis
    Michael A Kohanski
    Howard Hughes Medical Institute, Boston University, Boston, MA 02215, USA
    Mol Cell 37:311-20. 2010
    ..Here, we show that sublethal levels of bactericidal antibiotics induce mutagenesis, resulting in heterogeneous increases in the minimum inhibitory concentration for a range of antibiotics, ..
  44. pmc MmpL8 is required for sulfolipid-1 biosynthesis and Mycobacterium tuberculosis virulence
    Scott E Converse
    Department of Microbiology and Immunology, G W Hooper Foundation, University of California, San Francisco 94143, USA
    Proc Natl Acad Sci U S A 100:6121-6. 2003
    ....
  45. pmc Spontaneous mutagenesis is enhanced in Apex heterozygous mice
    Jessica Huamani
    Department of Cellular and Structural Biology, The University of Texas Health Science Center at San Antonio, 78229 3900, USA
    Mol Cell Biol 24:8145-53. 2004
    ..These data indicate that diminution of AP endonuclease has a significant effect on spontaneous mutagenesis in somatic and germ line cells.
  46. pmc Generation of a nicking enzyme that stimulates site-specific gene conversion from the I-AniI LAGLIDADG homing endonuclease
    Audrey McConnell Smith
    Division of Basic Sciences, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue, North Seattle, WA 98109, USA
    Proc Natl Acad Sci U S A 106:5099-104. 2009
    ..The development of sequence-specific nicking enzymes like the I-AniI nickase will facilitate comparative analyses of DNA repair and mutagenesis induced by single- or double-strand breaks.
  47. ncbi Oxidative damage to DNA: formation, measurement and biochemical features
    Jean Cadet
    Laboratoire Lesions des Acides Nucleiques, SCIB DRFMC and FRE 2600, CEA Grenoble, F 38054 Grenoble Cedex 9, France
    Mutat Res 531:5-23. 2003
    ..For this purpose modified oligonucleotides in which cyclopurine, and cyclopyrimidine nucleosides were site-specifically inserted were synthesized...
  48. pmc Efficient genome-wide mutagenesis of zebrafish genes by retroviral insertions
    Dongmei Wang
    Key Laboratory of Cell Proliferation and Differentiation, Center of Developmental Biology and Genetics, College of Life Sciences, Peking University, Ministry of Education, Beijing 100871, China
    Proc Natl Acad Sci U S A 104:12428-33. 2007
    ..Combining this increase in efficiency with cryopreservation of sperm samples from the F(1) fish, it is now possible to create a stable resource that contains mutations in every known zebrafish gene...
  49. ncbi Iterative saturation mutagenesis (ISM) for rapid directed evolution of functional enzymes
    Manfred T Reetz
    Max Planck Institut fur Kohlenforschung, Kaiser Wilhelm Platz 1, 45470 Mulheim Ruhr, Germany
    Nat Protoc 2:891-903. 2007
    Iterative saturation mutagenesis (ISM) is a new and efficient method for the directed evolution of functional enzymes. It reduces the necessary molecular biological work and the screening effort drastically...
  50. pmc Large-scale transposon mutagenesis of Mycoplasma pulmonis
    Christopher T French
    Department of Genetics, University of Alabama Birmingham, Birmingham, AL 35294, USA
    Mol Microbiol 69:67-76. 2008
    ..A mutant with a defect in transport of N-acetylglucosamine was identified...
  51. ncbi Broad antiretroviral defence by human APOBEC3G through lethal editing of nascent reverse transcripts
    Bastien Mangeat
    Department of Genetics and Microbiology, University of Geneva, 1211 Geneva 4, Switzerland
    Nature 424:99-103. 2003
    ..We also find that APOBEC3G can act on a broad range of retroviruses in addition to HIV, suggesting that hypermutation by editing is a general innate defence mechanism against this important group of pathogens...
  52. ncbi An ENU-induced mutation in the Ankrd11 gene results in an osteopenia-like phenotype in the mouse mutant Yoda
    Ivana Barbaric
    MRC Mammalian Genetics Unit, Harwell
    Physiol Genomics 32:311-21. 2008
    ..As an effort toward identifying novel genes involved in regulation of bone mass, N-ethyl-N-nitrosourea (ENU) mutagenesis in mice has been utilized...
  53. ncbi Tight steric closure at the intracellular activation gate of a voltage-gated K(+) channel
    D del Camino
    Department of Neurobiology, Harvard Medical School, 220 Longwood Avenue, Boston, MA 02115, USA
    Neuron 32:649-56. 2001
    ..Either these Kv channels have a broader inner entrance than seen in the KcsA crystal, even in the closed state, or the region is highly flexible (but nevertheless remains very securely closed nearby)...
  54. doi Unravelling UVA-induced mutagenesis
    Evelyne Sage
    Institut Curie, CNRS UMR3348, Universite de Paris Sud XI, F 91405, Orsay Cedex, France
    Photochem Photobiol Sci 11:74-80. 2012
    ..This may help unravel the role of UVA in the various steps of photocarcinogenesis. The connection to photocarcinogenesis is more extensively discussed by other authors in this issue...
  55. pmc The cytidine deaminase CEM15 induces hypermutation in newly synthesized HIV-1 DNA
    Hui Zhang
    The Dorrance H Hamilton Laboratories, Center for Human Virology and Biodefense, Division of Infectious Diseases and Environmental Medicine, Department of Medicine, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    Nature 424:94-8. 2003
    ..Importantly, the accumulation of CEM15-mediated non-lethal hypermutation in the replicating viral genome could potently contribute to the genetic variation of primate lentiviral populations...
  56. pmc Cre-lox-based system for multiple gene deletions and selectable-marker removal in Lactobacillus plantarum
    Jolanda M Lambert
    Wegeningen Centre for Food Science, Microbial Functionality and Safety Programme, Health and Safety Department, P O Box 20, 6710 BA Ede, The Netherlands
    Appl Environ Microbiol 73:1126-35. 2007
    ..First, an effective mutagenesis vector (pNZ5319) was constructed that allows direct cloning of blunt-end PCR products representing homologous ..
  57. pmc Systematic dissection and trajectory-scanning mutagenesis of the molecular interface that ensures specificity of two-component signaling pathways
    Emily J Capra
    Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA
    PLoS Genet 6:e1001220. 2010
    ..To explore how a relatively small set of residues can dictate partner selectivity, we combined alanine-scanning mutagenesis with an approach we call trajectory-scanning mutagenesis, in which all mutational intermediates between the ..
  58. pmc Chemical biology of mutagenesis and DNA repair: cellular responses to DNA alkylation
    Nidhi Shrivastav
    Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Carcinogenesis 31:59-70. 2010
    ..Here, we describe the respective contributions of specific DNA lesions to the biological effects of low molecular weight alkylating agents...
  59. doi An evaluation of Poisson-Boltzmann electrostatic free energy calculations through comparison with experimental mutagenesis data
    Ronald D Gorham
    Department of Bioengineering, University of California, Riverside, CA 92521, USA
    Biopolymers 95:746-54. 2011
    ..Using experimental or theoretical alanine-scanning mutagenesis, it is possible to elucidate the contribution of individual ionizable amino acids to protein association...
  60. pmc Targeted mutagenesis of duplicated genes in soybean with zinc-finger nucleases
    Shaun J Curtin
    Department of Agronomy and Plant Genetics, University of Minnesota, St Paul, Minnesota 55108, USA
    Plant Physiol 156:466-73. 2011
    We performed targeted mutagenesis of a transgene and nine endogenous soybean (Glycine max) genes using zinc-finger nucleases (ZFNs)...
  61. pmc Allosteric features of KCNQ1 gating revealed by alanine scanning mutagenesis
    Li Juan Ma
    Institut für Neurale Signalverarbeitung, Zentrum für Molekulare Neurobiologie, Universitat Hamburg, Hamburg, Germany
    Biophys J 100:885-94. 2011
    ..We applied alanine scanning mutagenesis to the S4-S5 linker and pore region and followed the regularities of gating perturbations in KCNQ1...
  62. ncbi ENU-based gene-driven mutagenesis in the mouse: a next-generation gene-targeting system
    Yoichi Gondo
    Mutagenesis and Genomics Team, RIKEN BioResource Center, Ibaraki, Japan
    Exp Anim 59:537-48. 2010
    As a new mouse mutant resource, the RIKEN ENU-based gene-driven mutagenesis system in the mouse has been available to the research community since 2002...
  63. doi An enhanced system for unnatural amino acid mutagenesis in E. coli
    Travis S Young
    Department of Chemistry and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    J Mol Biol 395:361-74. 2010
    ..afforded increased plasmid stability compared with previously reported vectors for unnatural amino acid mutagenesis. To demonstrate the utility of this new system, we introduced 14 mutant aaRS into pEVOL and compared their ..
  64. pmc High-resolution melting curve analysis for rapid detection of mutations in a Medaka TILLING library
    Tomoko Ishikawa
    Department of Radiation Biology and Medical Genetics, Graduate School of Medicine, Osaka University, B4, 2 2 Yamadaoka, Suita, Osaka 565 0871, Japan
    BMC Mol Biol 11:70. 2010
    ..Targeting Induced Local Lesions IN Genomes) is a reverse-genetic strategy that combines random chemical mutagenesis with high-throughput discovery of the induced mutations in target genes...
  65. pmc Hematopoietic stem cell quiescence promotes error-prone DNA repair and mutagenesis
    Mary Mohrin
    The Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research, Department of Medicine, Division of Hematology Oncology, University of California San Francisco, San Francisco, CA 94143, USA
    Cell Stem Cell 7:174-85. 2010
    ..Our results demonstrate that quiescence is a double-edged sword that renders HSCs intrinsically vulnerable to mutagenesis following DNA damage.
  66. pmc Structural interpretation of P2X receptor mutagenesis studies on drug action
    Richard J Evans
    Cell Physiology and Pharmacology, University of Leicester, Leicester, UK
    Br J Pharmacol 161:961-71. 2010
    ..b>Mutagenesis based studies have been used to develop an understanding of the molecular basis of their pharmacology with the ..
  67. pmc Highly mutagenic and severely imbalanced dNTP pools can escape detection by the S-phase checkpoint
    Dinesh Kumar
    Department of Medical Biochemistry and Biophysics, Umea University, SE 90187 Umea, Sweden
    Nucleic Acids Res 38:3975-83. 2010
    ..In contrast, when none of the dNTPs was limiting for DNA replication, even extreme and mutagenic dNTP pool imbalances did not activate the S-phase checkpoint and did not interfere with the cell cycle progression...
  68. doi Non-B DNA conformations as determinants of mutagenesis and human disease
    Albino Bacolla
    Center for Genome Research, Institute of Biosciences and Technology, Texas A and M University System Health Science Center, Texas Medical Center, 2121 W Holcombe Blvd, Houston, TX 77030, USA
    Mol Carcinog 48:273-85. 2009
    ..SSR polymorphic alleles in the population are associated with cancer susceptibility, including within genes that appear to share regulatory circuits involving reactive oxygen species...
  69. pmc Escherichia coli DNA polymerase IV mutator activity: genetic requirements and mutational specificity
    J Wagner
    Division of Genetics and Mutagenesis, National Institute of Health Sciences, Setagaya Ku, Tokyo 158 8501, Japan
    J Bacteriol 182:4587-95. 2000
    The dinB gene of Escherichia coli is known to be involved in the untargeted mutagenesis of lambda phage...
  70. pmc In vivo transcriptional profiling of Listeria monocytogenes and mutagenesis identify new virulence factors involved in infection
    Ana Camejo
    IBMC, Instituto de Biologia Molecular e Celular, Group of Molecular Microbiology, Universidade do Porto, Porto, Portugal
    PLoS Pathog 5:e1000449. 2009
    ..b>Mutagenesis of in vivo induced genes allowed the identification of novel L...
  71. pmc A rich TILLING resource for studying gene function in Brassica rapa
    Pauline Stephenson
    Department of Crop Genetics, John Innes Centre, Norwich, NR4 7UH, UK
    BMC Plant Biol 10:62. 2010
    ..rapa. Moreover, the ongoing sequencing of the B. rapa genome makes this species a highly attractive model for Brassica research and genetic resource development...
  72. pmc Hierarchy of lesion processing governs the repair, double-strand break formation and mutability of three-lesion clustered DNA damage
    Laura J Eccles
    CRUK MRC Gray Institute for Radiation Oncology and Biology, University of Oxford, Old Road Campus Research Building, Roosevelt Drive, Oxford, OX3 7DQ, UK
    Nucleic Acids Res 38:1123-34. 2010
    ..The inaccurate repair of such clusters has biological significance due to the ultimate risk of tumourigenesis or as potential cytotoxic lesions in tumour cells...
  73. ncbi Herpesvirus of turkey reconstituted from bacterial artificial chromosome clones induces protection against Marek's disease
    Susan J Baigent
    Institute for Animal Health, Compton Laboratory, Compton, Newbury, Berkshire RG20 7NN, UK
    J Gen Virol 87:769-76. 2006
    ..Using a one-step mutagenesis protocol to delete the essential glycoprotein B gene from the HVT genome, followed by construction of the ..
  74. ncbi Essential role for de novo DNA methyltransferase Dnmt3a in paternal and maternal imprinting
    Masahiro Kaneda
    Division of Human Genetics, Department of Integrated Genetics, National Institute of Genetics, Research Organization of Information and Systems, Mishima 411 8540, Japan
    Nature 429:900-3. 2004
    ..These results indicate that both Dnmt3a and Dnmt3L are required for methylation of most imprinted loci in germ cells, but also suggest the involvement of other factors...
  75. pmc Rab9 GTPase is required for replication of human immunodeficiency virus type 1, filoviruses, and measles virus
    James L Murray
    National Center for HIV, STD, and TB Prevention, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    J Virol 79:11742-51. 2005
    ..By using gene trap insertional mutagenesis, we identified Rab9, which mediates late-endosome-to-trans-Golgi-network trafficking, among several candidate ..
  76. ncbi A calcineurin-like gene ppb1+ in fission yeast: mutant defects in cytokinesis, cell polarity, mating and spindle pole body positioning
    T Yoshida
    Department of Biophysics, Faculty of Science, Kyoto University, Japan
    J Cell Sci 107:1725-35. 1994
    ..Thus the ppb1+ gene appears to be involved in cytokinesis, mating, transport, nuclear and spindle pole body positioning, and cell shape...
  77. pmc Mutagenesis of the bacterial RNA polymerase alpha subunit for improvement of complex phenotypes
    Daniel Klein-Marcuschamer
    Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Appl Environ Microbiol 75:2705-11. 2009
    ..Although the preferred method has traditionally been mutagenesis and selection, our laboratory has successfully used mutant transcription factors, which direct the RNA ..
  78. ncbi Y-family DNA polymerases in Escherichia coli
    Daniel F Jarosz
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Trends Microbiol 15:70-7. 2007
    The observation that mutations in the Escherichia coli genes umuC+ and umuD+ abolish mutagenesis induced by UV light strongly supported the counterintuitive notion that such mutagenesis is an active rather than passive process...
  79. ncbi Cell-to-cell movement of potato potexvirus X is dependent on suppression of RNA silencing
    Elizabeth H Bayne
    The Sainsbury Laboratory, John Innes Centre, Colney Lane, Norwich NR4 7UH, UK
    Plant J 44:471-82. 2005
    ..It is possible, based on analyses of short interfering RNA accumulation, that P25 suppresses silencing by interfering with either assembly or function of the effector complexes of RNA silencing...
  80. pmc TILLING in Lotus japonicus identified large allelic series for symbiosis genes and revealed a bias in functionally defective ethyl methanesulfonate alleles toward glycine replacements
    Jillian Perry
    Sainsbury Laboratory, Norwich NR4 7UH, United Kingdom
    Plant Physiol 151:1281-91. 2009
    ..Our results demonstrate the power, as well as the limitations, of ethyl methanesulfonate mutagenesis for forward and reverse genetic studies. (Original mutant phenotypes can be accessed at http://data.jic.bbsrc...
  81. ncbi Sanpodo and Notch act in opposition to Numb to distinguish sibling neuron fates in the Drosophila CNS
    J B Skeath
    Department of Cell and Structural Biology, Howard Hughes Medical Institute, University of Illinois, Urbana, Illinois 61801, USA
    Development 125:1857-65. 1998
    ..Taken together, these results show that interactions between sanpodo, the Notch signaling pathway and numb enable CNS sibling neurons to acquire different fates...
  82. pmc Regions of the amino terminus of the P2X receptor required for modification by phorbol ester and mGluR1alpha receptors
    Hairuo Wen
    Department of Cell Physiology and Pharmacology, University of Leicester, Leicester, UK
    J Neurochem 108:331-40. 2009
    ..These results demonstrate that the conserved YXTXK/R sequence and a region with a conserved glycine residue close to the first transmembrane segment contribute to PMA and GPCR regulation of P2X(1) receptors...
  83. pmc Whole-body sleeping beauty mutagenesis can cause penetrant leukemia/lymphoma and rare high-grade glioma without associated embryonic lethality
    Lara S Collier
    Department of Genetics, Masonic Cancer Center and Masonic Cancer Center Histopathology Core, University of Minnesota, Minneapolis, Minnesota, USA
    Cancer Res 69:8429-37. 2009
    ..or deletions, indicating that transposon mobilization likely promotes tumor formation by insertional mutagenesis of cancer genes, and not by promoting wide-scale genomic instability...
  84. ncbi Glycosylation broadens the substrate profile of membrane type 1 matrix metalloproteinase
    Yi I Wu
    Department of Cell and Molecular Biology and Division of Hematology Oncology, Department of Medicine, Feinberg School of Medicine, Northwestern University, Chicago, Illinois, USA
    J Biol Chem 279:8278-89. 2004
    ..Enzymatic deglycosylation, site-directed mutagenesis, and lectin precipitation assays were used to demonstrate that MT1-MMP contains O-linked complex carbohydrates ..
  85. ncbi Premature ageing in mice expressing defective mitochondrial DNA polymerase
    Aleksandra Trifunovic
    Department of Medical Nutrition, Karolinska Institutet, Novum, Karolinska University Hospital, S 141 86 Stockholm, Sweden
    Nature 429:417-23. 2004
    ..Our results thus provide a causative link between mtDNA mutations and ageing phenotypes in mammals...
  86. ncbi Establishing gene function by mutagenesis in Arabidopsis thaliana
    Lars Østergaard
    Section of Cell and Developmental Biology, University of California at San Diego, La Jolla, CA 92093 0116, USA
    Plant J 39:682-96. 2004
    ..The first class typically includes point mutations or small deletions derived from chemical or fast neutron mutagenesis whereas the second class includes insertions of transferred-DNA or transposon elements...
  87. pmc UmuD and RecA directly modulate the mutagenic potential of the Y family DNA polymerase DinB
    Veronica G Godoy
    Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Mol Cell 28:1058-70. 2007
    ..by interaction with these proteins, thereby preventing the template bulging responsible for -1 frameshift mutagenesis. Intriguingly, residues that define the UmuD(2)-interacting surface on DinB statistically covary throughout ..
  88. pmc Cloning and expression in Escherichia coli of the OGG1 gene of Saccharomyces cerevisiae, which codes for a DNA glycosylase that excises 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine
    P A Van Der Kemp
    Unité de Recherche Associée 147 Centre National de la Recherche Scientifique, Villejuif, France
    Proc Natl Acad Sci U S A 93:5197-202. 1996
    ..E. coli (fpg mutY) was transformed by a yeast DNA library, and clones that showed a reduced spontaneous mutagenesis were selected. The antimutator activity was associated with pYSB10, an 11-kbp recombinant plasmid...
  89. pmc Construction of a self-excisable bacterial artificial chromosome containing the human cytomegalovirus genome and mutagenesis of the diploid TRL/IRL13 gene
    Dong Yu
    Department of Molecular Biology, Princeton University, Princeton, NJ 08544 1014, USA
    J Virol 76:2316-28. 2002
    ..We performed site-directed mutagenesis on pAD/Cre to generate a series of viruses in which the TRL/IRL13 diploid genes were disrupted and subsequently ..
  90. pmc Translation elongation factor 1A facilitates the assembly of the tombusvirus replicase and stimulates minus-strand synthesis
    Zhenghe Li
    Department of Plant Pathology, University of Kentucky, Lexington, Kentucky, United States of America
    PLoS Pathog 6:e1001175. 2010
    ..These roles for eEF1A are separate from its canonical role in host and viral protein translation, emphasizing critical functions for this abundant cellular protein during TBSV replication...
  91. pmc Direct demonstration of half-of-the-sites reactivity in the dimeric cytochrome bc1 complex: enzyme with one inactive monomer is fully active but unable to activate the second ubiquinol oxidation site in response to ligand binding at the ubiquinone reducti
    Michela Castellani
    Institute of Biochemistry, Molecular Genetics, Goethe University and Cluster of Excellence Macromolecular Complexes Frankfurt am Main, D 60438 Frankfurt am Main, Germany
    J Biol Chem 285:502-10. 2010
    ....
  92. ncbi Scanning cysteine mutagenesis analysis of Abeta-(1-40) amyloid fibrils
    Shankaramma Shivaprasad
    Graduate School of Medicine, University of Tennessee, Knoxville, 37920, USA
    J Biol Chem 281:993-1000. 2006
    ..Residues 20, 30, and 35, flanking these implicated beta-sandwich regions, are accessible to alkylation in the fibril indicating a location in solvent exposed structure...
  93. pmc Human RECQ helicases: roles in DNA metabolism, mutagenesis and cancer biology
    Raymond J Monnat
    Department of Pathology, University of Washington, Seattle, WA 98195 7705, USA
    Semin Cancer Biol 20:329-39. 2010
    ..I also discuss the emerging role for RECQ helicases as predictors of disease risk and the response to therapy...
  94. pmc Efficient gene targeting in Drosophila by direct embryo injection with zinc-finger nucleases
    Kelly J Beumer
    Department of Biochemistry, University of Utah School of Medicine, 15 North Medical Drive East, Salt Lake City, UT 84112 5650, USA
    Proc Natl Acad Sci U S A 105:19821-6. 2008
    ..Both local mutagenesis via nonhomologous end joining (NHEJ) and targeted gene replacement via homologous recombination (HR) have been ..
  95. doi Mutagenesis and homology modeling of the Tn21 integron integrase IntI1
    Carolina Johansson
    Department of Medical Biochemistry and Microbiology, Uppsala University, BMC, Box 582, 751 23 Uppsala, Sweden
    Biochemistry 48:1743-53. 2009
    ..to interpret the roles of several amino acid residues, four of which were clearly binding assay responsive upon mutagenesis. Notably, we also observed features indicating that IntI1 may be more prone to base-specific contacts with ..
  96. pmc Analysis by mutagenesis of a chromosomal integron integrase from Shewanella amazonensis SB2BT
    André Larouche
    Centre de Recherche en Infectiologie, Centre Hospitalier Universitaire de Quebec, Quebec, Canada
    J Bacteriol 191:1933-40. 2009
    ..Our results also suggest that the cysteine residue in the beta-5 strand is essential to the activity of Shewanella-type integrases, while the cysteine in the beta-4 strand is less important for the excision activity...
  97. pmc Identification of residues in the drug translocation pathway of the human multidrug resistance P-glycoprotein by arginine mutagenesis
    Tip W Loo
    Department of Medicine, University of Toronto, Toronto, Ontario M5S 1A8, Canada
    J Biol Chem 284:24074-87. 2009
    ..To map the residues in the drug translocation pathway, we performed arginine-scanning mutagenesis on all transmembrane (TM) segments (total = 237 residues) of a P-gp processing mutant (G251V) defective in ..
  98. pmc A transposon toolkit for gene transfer and mutagenesis in protozoan parasites
    Jeziel D Damasceno
    Departamento de Biologia Celular e Molecular e Bioagentes Patogênicos, Faculdade de Medicina de Ribeirao Preto, Universidade de Sao Paulo, Ribeirao Preto, SP, Brazil
    Genetica 138:301-11. 2010
    ..The piggyBac and Tn5 transposons have also been shown to mobilize across Plasmodium spp. genomes circumventing the current limitations in the genetic manipulation of these organisms...
  99. ncbi Cysteine-scanning mutagenesis of serotonin transporter intracellular loop 2 suggests an alpha-helical conformation
    Yuan Wei Zhang
    Department of Pharmacology, Yale University School of Medicine, New Haven, Connecticut 06520 8066, USA
    J Biol Chem 280:30807-13. 2005
    ..A 24-residue region of SERT including IL2, from Ile-270 through Ser-293, was analyzed by cysteine-scanning mutagenesis and chemical modification...
  100. pmc Alanine-shaving mutagenesis to determine key interfacial residues governing the assembly of a nano-cage maxi-ferritin
    Yu Zhang
    Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University, Singapore
    J Biol Chem 285:12078-86. 2010
    ..from Escherichia coli, a maxi-ferritin made up of 24 subunits, was chosen as the basis for an alanine-shaving mutagenesis study to discover key amino acid residues at symmetry-related protein-protein interfaces that control protein ..
  101. ncbi Functional genetic analysis of the mammalian mitochondrial DNA encoded peptides: a mutagenesis approach
    Maria Pilar Bayona-Bafaluy
    Departamento de Bioquimica y Biologia Molecular y Celular, Universidad de Zaragoza, Zaragoza, Spain
    Methods Mol Biol 457:379-90. 2008
    ..Chemical mutagenesis followed by mtDNA copy number reduction and the use of large-scale negative selection in duplicate cultures, ..

Research Grants85

  1. Functional Variomics and Applications
    Xuewen Pan; Fiscal Year: 2009
    ..model organisms, with potentially numerous variant alleles being pre-made for each gene in a genome by random mutagenesis and used for isolating alleles that confer phenotypes of interest...
  2. National Biomedical EPR Center
    James Hyde; Fiscal Year: 2003
    ..It is proposed to develop a resonator for aqueous samples in the context of site-directed mutagenesis. Specific problems with current resonators from Bruker BioSpin are identified and plans to circumvent them are ..
  3. STRUCTURE, BIOSYNTHESIS AND FUNCTION OF GLYCOPROTEINS
    Stuart Kornfeld; Fiscal Year: 2007
    ..We will use site-directed mutagenesis to identify residues on the surface of DNase I that determine binding to phosphotransferase and analyze how ..
  4. CHARACTERIZATION OF NEUROPILIN, A NOVEL VEGF RECEPTOR
    Michael Klagsbrun; Fiscal Year: 2007
    ..ligand binding sites are unique or overlapping by analysis of deletion mutants and site-directed[unreadable] mutagenesis; ii) structural analysis of the heparin that binds NRP blb2 in collaboration with Dr John Gallagher (University ..
  5. Pilot and definitive studies to evaluate Insertional Mutagenesis
    LU TAYLOR; Fiscal Year: 2012
    ..FDA-NTP Studies of Insertional Mutagenesis - Pilot Study: 12-week pilot study has been awarded to the lab to develop and validate preclinical model for ..
  6. REACTION MECHANISM OF MYOCARDIAC UBIQUINONE-PROTEINS
    Chang An Yu; Fiscal Year: 2007
    ..During past support periods we have established protocols for site-directed mutagenesis coupled with in vivo and in vitro reconstitutionto study some of the structural and functional relationships in ..
  7. REACTION MECHANISM OF MYOCARDIAC UBIQUINONE-PROTEINS
    Chang An Yu; Fiscal Year: 2009
    ..During past support periods we have established protocols for site-directed mutagenesis coupled with in vivo and in vitro reconstitutionto study some of the structural and functional relationships in ..
  8. The role of human DNA polymerase eta in the mutagenic response to oxidative stres
    SCOTT DAVID MCCULLOCH; Fiscal Year: 2010
    ..A similar logic has been proposed for the suppression of mutagenesis during pol 7 dependent bypass of 8-oxo-dG...
  9. The role of human DNA polymerase eta in the mutagenic response to oxidative stres
    SCOTT MCCULLOCH; Fiscal Year: 2009
    ..A similar logic has been proposed for the suppression of mutagenesis during pol 7 dependent bypass of 8-oxo-dG...
  10. Generation of Site-Specific Recombinase-Expressing Transgenic Rats using an Enhan
    Eric M Ostertag; Fiscal Year: 2010
    ..While in vitro gene trap mutagenesis is useful for gene discovery, a rapid and efficient in vivo method is preferable for two key reasons: 1) an in ..
  11. Generation of Site-Specific Recombinase-Expressing Transgenic Rats using an Enhan
    Eric M Ostertag; Fiscal Year: 2011
    ..While in vitro gene trap mutagenesis is useful for gene discovery, a rapid and efficient in vivo method is preferable for two key reasons: 1) an in ..
  12. MECHANISMS IN THE REGULATION OF SP-A GENE EXPRESSION
    CAROLE MENDELSON; Fiscal Year: 2002
    ..In deletion mapping and mutagenesis studies, we have found that basal and cAMP induction of rabbit and human SP-A promoter activity is critically ..
  13. MECHANISMS IN THE REGULATION OF SP-A GENE EXPRESSION
    CAROLE MENDELSON; Fiscal Year: 1999
    ..In deletion mapping and mutagenesis studies, we have found that basal and cAMP induction of rabbit and human SP-A promoter activity is critically ..
  14. MECHANISMS IN THE REGULATION OF SP-A GENE EXPRESSION
    CAROLE MENDELSON; Fiscal Year: 2000
    ..In deletion mapping and mutagenesis studies, we have found that basal and cAMP induction of rabbit and human SP-A promoter activity is critically ..
  15. MECHANISMS IN THE REGULATION OF SP-A GENE EXPRESSION
    CAROLE MENDELSON; Fiscal Year: 2001
    ..In deletion mapping and mutagenesis studies, we have found that basal and cAMP induction of rabbit and human SP-A promoter activity is critically ..
  16. MECHANISMS IN THE REGULATION OF SP-A GENE EXPRESSION
    CAROLE MENDELSON; Fiscal Year: 2007
    ..In deletion mapping and mutagenesis studies, we have[unreadable] found that basal and cAMP induction of rabbit and human SP-A promoter[unreadable] ..
  17. Identification of Coxiella burnetii Virulence Factors by Transposon Mutagenesis
    JAMES EVANS SAMUEL; Fiscal Year: 2010
    ..outside of host cells;b) novel techniques for efficient clonal isolation;c) functional Himar1-based transposon mutagenesis system to generate random mutations;and d) transformation with a stable shuttle vector plasmid...
  18. METAL MUTAGENESIS IN VITRO--POLYMERASE KINETICS
    Elizabeth Snow; Fiscal Year: 1993
    The goals of this research are two-fold: to determine the mechanisms of mutagenesis induced by the carcinogenic metals nickel and chromium and to use Ni(II) and Cr(III) ions as tools to investigate the enzymatic mechanisms responsible ..
  19. Monoamine Transporter Structure-Function Studies
    CHRISTOPHER SURRATT; Fiscal Year: 2007
    ..create LeuTAa-based 3-D DAT and SERT models, and use the models to identify and characterize via site-directed mutagenesis and pharmacology the DAT and SERT residues key in substrate (e.g...
  20. Genetic Basis of Myeloid Neoplasia--Mutagenesis Screens
    A Look; Fiscal Year: 2005
    ..This proposal tests two linked hypotheses: (i) genome-wide ethylnitrosourea (ENU) mutagenesis screens in the zebrafish can be used to identify dominant and recessive mutations that cause a deficiency or ..
  21. Genetic Basis of Myeloid Neoplasia--Mutagenesis Screens
    A Look; Fiscal Year: 2001
    ..This proposal tests two linked hypotheses: (i) genome-wide ethylnitrosourea (ENU) mutagenesis screens in the zebrafish can be used to identify dominant and recessive mutations that cause a deficiency or ..
  22. Genetic Basis of Myeloid Neoplasia--Mutagenesis Screens
    A Look; Fiscal Year: 2002
    ..This proposal tests two linked hypotheses: (i) genome-wide ethylnitrosourea (ENU) mutagenesis screens in the zebrafish can be used to identify dominant and recessive mutations that cause a deficiency or ..
  23. Genetic Basis of Myeloid Neoplasia--Mutagenesis Screens
    A Look; Fiscal Year: 2003
    ..This proposal tests two linked hypotheses: (i) genome-wide ethylnitrosourea (ENU) mutagenesis screens in the zebrafish can be used to identify dominant and recessive mutations that cause a deficiency or ..
  24. Genetic Basis of Myeloid Neoplasia--Mutagenesis Screens
    A Look; Fiscal Year: 2004
    ..This proposal tests two linked hypotheses: (i) genome-wide ethylnitrosourea (ENU) mutagenesis screens in the zebrafish can be used to identify dominant and recessive mutations that cause a deficiency or ..
  25. Creation of hyperactive transposons for mutagenesis in rodents
    Eric M Ostertag; Fiscal Year: 2010
    ..PUBLIC HEALTH RELEVANCE: In the application "Creation of hyperactive transposases for mutagenesis in rodents," we are seeking Phase II funding to use the novel transposases we created to generate new models of ..
  26. OXIDATIVE ACTIVATION OF QUINONES AND MUTAGENESIS
    Dhrubajyoti Chakravarti; Fiscal Year: 2002
    ..hypothesize that these carcinogens (diethylstilbestrol and benzene) and dopamine cause disease through specific mutagenesis, induce mutations by error-prone repair...
  27. GENETICS OF MYELOID NEOPLASIA--MUTAGENESIS IN ZEBRAFISH
    A Look; Fiscal Year: 2002
    ..This proposal tests two linked hypotheses: (i) genome-wide ethylnitrosourea (ENU) mutagenesis screens in the zebrafish can be used to identify dominant and recessive mutations that cause a deficiency or ..
  28. MUTAGENESIS OF SPECIFIC REGIONS OF THE SV 40 GENOME
    MARY TEVETHIA; Fiscal Year: 2000
    ....
  29. MUTAGENESIS OF SPECIFIC REGIONS OF THE SV 40 GENOME
    MARY TEVETHIA; Fiscal Year: 2001
    ....
  30. MUTAGENESIS OF SPECIFIC REGIONS OF THE SV 40 GENOME
    MARY TEVETHIA; Fiscal Year: 1999
    ....
  31. Genetic analysis of Cryptococcus neoformans virulence
    Joseph Heitman; Fiscal Year: 2005
    ..We propose to broadly apply Insertional mutagenesis to identify genes encoding virulence attributes necessary for infection. C...
  32. Genetic analysis of Cryptococcus neoformans virulence
    Joseph Heitman; Fiscal Year: 2007
    ..We propose to broadly apply Insertional mutagenesis to identify genes encoding virulence attributes necessary for infection. C...
  33. Genetic analysis of Cryptococcus neoformans virulence
    Joseph Heitman; Fiscal Year: 2006
    ..We propose to broadly apply Insertional mutagenesis to identify genes encoding virulence attributes necessary for infection. C...
  34. Genetic analysis of Cryptococcus neoformans virulence
    Joseph Heitman; Fiscal Year: 2006
    ..We propose to broadly apply Insertional mutagenesis to identify genes encoding virulence attributes necessary for infection. C...
  35. Genetic analysis of Cryptococcus neoformans virulence
    Joseph Heitman; Fiscal Year: 2009
    ..We propose to broadly apply Insertional mutagenesis to identify genes encoding virulence attributes necessary for infection. C...
  36. OXYGEN AND SPONTANEOUS MUTAGENESIS IN ESCHERICHIA COLI
    Robert Fowler; Fiscal Year: 1991
    ..superoxide radical, hydrogen peroxide and hydroxyl radical, are thought to be possible causative agents in mutagenesis, carcinogenesis and aging...
  37. Error Correction in DNA Synthesis: A Biochemical Study
    MYRON FREDRIC GOODMAN; Fiscal Year: 2011
    ..While investigating the biochemical basis of SOS damaged- induced mutagenesis in E...
  38. Creation of hyperactive transposons for mutagenesis in rodents
    Eric Ostertag; Fiscal Year: 2009
    This project aims to rapidly produce rat mutants through a transposon-based method of mutagenesis. The rat is a favored model for many types of human disease for which mice are not suitable...
  39. Site-Directed Oxidative Modification of Muscle Protein Structural Dynamics
    David D Thomas; Fiscal Year: 2012
    ..This project employs site-directed mutagenesis for three purposes: (1) Met mutagenesis will be used to control susceptibility to oxidation, (2) previously ..
  40. RADIATION MUTAGENESIS AND REPAIR IN SACCHAROMYCES
    Christopher Lawrence; Fiscal Year: 1999
    ..Christopher Lawrence proposes to continue a long-term study on the mechanism of DNA-damage induced mutagenesis in S. cerevisiae...
  41. VOLTAGE-SENSITIVE SODIUM CHANNELS IN BRAIN
    William Catterall; Fiscal Year: 1992
    ..Structure-function studies with site-directed antibodies and site-directed mutagenesis have identified regions of the channel that are critical for voltage-dependent activation, fast channel ..
  42. VOLTAGE-SENSITIVE SODIUM CHANNELS IN BRAIN
    William Catterall; Fiscal Year: 1993
    ..Structure-function studies with site-directed antibodies and site-directed mutagenesis have identified regions of the channel that are critical for voltage-dependent activation, fast channel ..
  43. ANTICHYMOTRYPSIN INTERACTION WITH SERINE PROTEASES
    BARRY COOPERMAN; Fiscal Year: 1992
    ..interaction of ACT with Chtr and CatG, and to test our understanding by redesigning ACT, through site-specific mutagenesis, to change its specificity...
  44. ANTICHYMOTRYPSIN INTERACTION WITH SERINE PROTEASES
    BARRY COOPERMAN; Fiscal Year: 1991
    ..interaction of ACT with Chtr and CatG, and to test our understanding by redesigning ACT, through site-specific mutagenesis, to change its specificity...
  45. ANTICHYMOTRYPSIN INTERACTION WITH SERINE PROTEASES
    BARRY COOPERMAN; Fiscal Year: 1993
    ..interaction of ACT with Chtr and CatG, and to test our understanding by redesigning ACT, through site-specific mutagenesis, to change its specificity...
  46. Development of Site-Specific Mutagenesis in Coxiella burnetti
    Katja Mertens; Fiscal Year: 2011
    ..burnetii and usage of counter selection markers to force allelic exchange. (2) Development of site-specific mutagenesis in C. burnetii using allelic exchange. We will achieve allelic exchange in C...
  47. IONIZING RADIATION MUTAGENESIS IN ESCHERICHIA COLI
    KENDRIC SMITH; Fiscal Year: 1990
    One important finding from our studies on ionizing radiation mutagenesis (IRM). In Escherichia coli is that IRM is not uniformly dependent on the umuDC genes as is the case for UV radiation mutagenesis...
  48. MUTAGENESIS OF PYRIDOXAL PHOSPHATE-DEPENDENT ENZYMES
    JACK KIRSCH; Fiscal Year: 1993
    ..Site directed mutagenesis of aspartate aminotransferase will be based on computer modeling of the two homologous structures...
  49. Environmental Mutagen Society 2011 Annual Meeting
    CATHERINE G B KLEIN; Fiscal Year: 2011
    ..Society (EMS) is the primary scientific society fostering research on the basic mechanisms of DNA repair and mutagenesis and application of this knowledge to understanding human health effects from exposure to environmental toxins...
  50. STRUCTURE/FUNCTION STUDIES OF NA,K-ATPASE
    Elmer Price; Fiscal Year: 1992
    ..All of the aims of the proposal entail the use of in vitro mutagenesis to introduce amino acid substitutions in the a subunit of the Na,K-ATPase...
  51. TRANSCRIPTION/REPLICATION OF NEGATIVE STRAND RNA VIRUSES
    Sue Moyer; Fiscal Year: 1999
    ..We have located the L binding domain on P protein and propose to do site-directed mutagenesis in this domain of P to identify the amino acids critical for binding and RNA synthesis...
  52. MOLECULAR ANALYSIS OF CARCINOGEN-INDUCED MUTATIONS
    Norman Drinkwater; Fiscal Year: 1990
    ..basis for carcinogen-induced mutations, recombinant DNA shuttle vectors were developed for analysis of mutagenesis in human lymphoblastoid cells...
  53. MOLECULAR MECHANISMS OF CHROMIUM MUTAGENESIS
    Kathleen Dixon; Fiscal Year: 2000
    ..In addition, we are seeking a link between chromium mutagenesis and carcinogenesis by using transgenic mice to examine the mutagenic effects of inhaled calcium chromate dust ..
  54. MOLECULAR MECHANISMS OF CHROMIUM MUTAGENESIS
    Kathleen Dixon; Fiscal Year: 1999
    ..In addition, we are seeking a link between chromium mutagenesis and carcinogenesis by using transgenic mice to examine the mutagenic effects of inhaled calcium chromate dust ..
  55. RADIATION, ASBESTOS, AND MUTAGENESIS
    TOM HEI; Fiscal Year: 1991
    ..Quantitative mutagenesis assay systems allow an assessment of health risks for exposure to radiation and other environmental ..
  56. SITE-SPECIFIC MUTAGENESIS OF ISOMERASES
    Gregory Petsko; Fiscal Year: 1993
    The goal of this project is to use a combination of site-directed mutagenesis and X-ray crystallography to understand the structural basis of the catalytic power of yeast triosephosphate isomerase (TIM) and to unravel the mechanism of ..
  57. L1 retrotransposon-based mutagenesis for rat models of human diseases
    Eric Ostertag; Fiscal Year: 2009
    ..Unfortunately, many genetic manipulation techniques available in the mouse such as random mutagenesis with a gene trap (both retroviral-based and non-retroviral-based), gene knock- outs, gene knock-ins, and ..
  58. SITE-SPECIFIC MUTAGENESIS OF ISOMERASES
    Gregory Petsko; Fiscal Year: 1991
    The goal of this project is to use a combination of site-directed mutagenesis and X-ray crystallography to understand the structural basis of the catalytic power of yeast triosephosphate isomerase (TIM) and to unravel the mechanism of ..
  59. SITE-SPECIFIC MUTAGENESIS OF ISOMERASES
    Gregory Petsko; Fiscal Year: 1992
    The goal of this project is to use a combination of site-directed mutagenesis and X-ray crystallography to understand the structural basis of the catalytic power of yeast triosephosphate isomerase (TIM) and to unravel the mechanism of ..
  60. L1 retrotransposon-based mutagenesis for rat models of human diseases
    Eric Ostertag; Fiscal Year: 2007
    ..Unfortunately, many genetic manipulation techniques available in the mouse such as random mutagenesis with a gene trap (both retroviral-based and non-retroviral-based), gene knock- outs, gene knock-ins, and ..
  61. MUTAGENIC AND RECOMBINOGENIC EFFECTS OF GAMMA RAYS
    William Taylor; Fiscal Year: 1990
    ..find out which of the gamma-ray induced lesions in DNA are responsible for the biological effects; lethality, mutagenesis and chromosomal rearrangements...
  62. Experimental viral evolution at high mutation rate
    JAMES JEFFREY BULL; Fiscal Year: 2010
    ..of deleterious mutations;high rates can even cause population extinction, a process known as lethal mutagenesis. This principle underlies the common use of mutagenic, drugs to treat viral infections clinically...
  63. MOLECULAR BIOLOGY OF NEUROGENESIS
    Stephen Crews; Fiscal Year: 1999
    ..Mutations will be generated following standard genetic screens, including an EMS mutagenesis (using a deficiency shown to uncover the Darnt gene) and a P-element transposition mutagenesis...
  64. MOLECULAR BIOLOGY OF NEUROGENESIS
    Stephen Crews; Fiscal Year: 2000
    ..Mutations will be generated following standard genetic screens, including an EMS mutagenesis (using a deficiency shown to uncover the Darnt gene) and a P-element transposition mutagenesis...
  65. Stem cell mutagenesis mediated by age and niche
    VIVIENNE REBEL; Fiscal Year: 2009
    ..the different tissues is maintained during aging is limited largely to analyses of DNA damage, DNA repair, and mutagenesis in differentiated cells and tissues...
  66. Zebrafish ES cell lines for targeted mutagenesis
    Paul Collodi; Fiscal Year: 2009
    ..Although large-scale forward mutagenesis screens have been successfully applied to the zebrafish to identify genes that regulate early development and ..
  67. Zebrafish ES cell lines for targeted mutagenesis
    Paul Collodi; Fiscal Year: 2010
    ..Although large-scale forward mutagenesis screens have been successfully applied to the zebrafish to identify genes that regulate early development and ..
  68. Zebrafish ES cell lines for targeted mutagenesis
    Paul Collodi; Fiscal Year: 2006
    ..Although large-scale forward mutagenesis screens have been successfully applied to the zebrafish to identify genes that regulate early development and ..
  69. Zebrafish ES cell lines for targeted mutagenesis
    Paul Collodi; Fiscal Year: 2007
    ..Although large-scale forward mutagenesis screens have been successfully applied to the zebrafish to identify genes that regulate early development and ..
  70. DNA REPLICATION AFTER INSULT WITH UV
    THOMAS GRIFFITHS; Fiscal Year: 1990
    The project's long-term objectives are to help elucidate the molecular processes involved in carcinogenesis and mutagenesis. Specific aims relate to how the DNA lesions are handled in various eukaryotic cells...
  71. Suppression of Mutagenesis with Antioxidants
    WILFREDO HERNANDEZ; Fiscal Year: 2009
    ..theories of cancer highlight hypoxia in microenvironments where oxidative stress damages DNA and promotes mutagenesis. Our major aim is to evaluate suppression of mutagenesis by sulfhydryl antioxidant N-acetylcysteine (NAC), and ..
  72. SITE-SPECIFIC MUTAGENESIS OF THE CA2+-ATPASE
    David MacLennan; Fiscal Year: 1991
    ..The expression and mutagenesis of full length cDNA encoding the Ca2+ -ATPase, followed by assay of the function of the product, is being used ..
  73. MOLECULAR GENETICS AND MECHANISM OF PROTEIN ANTIBIOTICS
    J Hansen; Fiscal Year: 1993
    ..Recent studies in this laboratory have used site-directed mutagenesis of the subtilin prepeptide gene to construct and express a structural analog of subtilin, in which the change ..
  74. MOLECULAR GENETICS AND MECHANISM OF PROTEIN ANTIBIOTICS
    J Hansen; Fiscal Year: 2001
    ..Recent studies in this laboratory have used site-directed mutagenesis of the subtilin prepeptide gene to construct and express a structural analog of subtilin, in which the change ..
  75. MOLECULAR GENETICS AND MECHANISM OF PROTEIN ANTIBIOTICS
    J Hansen; Fiscal Year: 2000
    ..Recent studies in this laboratory have used site-directed mutagenesis of the subtilin prepeptide gene to construct and express a structural analog of subtilin, in which the change ..
  76. Suppression of Mutagenesis with Antioxidants
    WILFREDO HERNANDEZ; Fiscal Year: 2010
    ..theories of cancer highlight hypoxia in microenvironments where oxidative stress damages DNA and promotes mutagenesis. Our major aim is to evaluate suppression of mutagenesis by sulfhydryl antioxidant N-acetylcysteine (NAC), and ..
  77. MOLECULAR GENETICS AND MECHANISM OF PROTEIN ANTIBIOTICS
    J Hansen; Fiscal Year: 1999
    ..Recent studies in this laboratory have used site-directed mutagenesis of the subtilin prepeptide gene to construct and express a structural analog of subtilin, in which the change ..