Summary: The functional hereditary units of PROTOZOA.
Publications253 found, 100 shown here
- The genome of the African trypanosome Trypanosoma bruceiMatthew Berriman
Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton CB10 1SA, UK
Science 309:416-22. 2005..brucei and the greatest in L. major. Horizontal transfer of genes of bacterial origin has contributed to some of the metabolic differences in these parasites, and a number of novel potential drug targets have been identified...
- Genome of the host-cell transforming parasite Theileria annulata compared with T. parvaArnab Pain
Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
Science 309:131-3. 2005....
- The transcriptome of the intraerythrocytic developmental cycle of Plasmodium falciparumZbynek Bozdech
Department of Biochemistry and Biophysics, University of California, San Francisco, USA
PLoS Biol 1:E5. 2003..falciparum and provide a resource for the identification of new chemotherapeutic and vaccine candidates...
- Chloroquine resistance in Plasmodium falciparum malaria parasites conferred by pfcrt mutationsAmar bir Singh Sidhu
Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
Science 298:210-3. 2002..pfcrt mutations increased susceptibility to artemisinin and quinine and minimally affected amodiaquine activity; hence, these antimalarials warrant further investigation as agents to control chloroquine-resistant falciparum malaria...
- Plasmodium falciparum variant surface antigen expression patterns during malariaPeter C Bull
Nuffield Department of Clinical Medicine, John Radcliffe Hospital, University of Oxford, Oxford, United Kingdom
PLoS Pathog 1:e26. 2005....
- The genome sequence of Trypanosoma cruzi, etiologic agent of Chagas diseaseNajib M El-Sayed
Department of Parasite Genomics, Institute for Genomic Research, Rockville, MD 20850, USA
Science 309:409-15. 2005....
- Draft genome sequence of the sexually transmitted pathogen Trichomonas vaginalisJane M Carlton
Institute for Genomic Research, 9712 Medical Research Drive, Rockville, MD 20850, USA
Science 315:207-12. 2007..The genome sequence predicts previously unknown functions for the hydrogenosome, which support a common evolutionary origin of this unusual organelle with mitochondria...
- Widespread functional specialization of Plasmodium falciparum erythrocyte membrane protein 1 family members to bind CD36 analysed across a parasite genomeBridget A Robinson
Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80525, USA
Mol Microbiol 47:1265-78. 2003..These can be used to investigate the role of proteins in malaria pathogenesis and potentially tailor vaccines to recognize particular binding variants...
- Genomic minimalism in the early diverging intestinal parasite Giardia lambliaHilary G Morrison
Marine Biological Laboratory, Woods Hole, MA 02543 1015, USA
Science 317:1921-6. 2007..This genome sequence will not only be valuable for investigating the evolution of eukaryotes, but will also be applied to the search for new therapeutics for this parasite...
- P. falciparum rosetting mediated by a parasite-variant erythrocyte membrane protein and complement-receptor 1J A Rowe
Laboratory of Parasitic Diseases, NIAID, NIH, Bethesda, Maryland 20892, USA
Nature 388:292-5. 1997..Thus we describe a new adhesive function for PfEMP1 and raise the possibility that CR1 polymorphisms in Africans that influence the interaction between erythrocytes and PfEMP1 may protect against severe malaria...
- Genome sequence of Babesia bovis and comparative analysis of apicomplexan hemoprotozoaKelly A Brayton
Program in Genomics, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, USA
PLoS Pathog 3:1401-13. 2007..The genome sequence provides a greater understanding of B. bovis metabolism and potential avenues for drug therapies and vaccine development...
- A pol I transcriptional body associated with VSG mono-allelic expression in Trypanosoma bruceiM Navarro
Present address Instituto de Parasitología y Biomedicina López Neyra, CSIC, C Ventanilla 11, 18001 Granada, Spain
Nature 414:759-63. 2001..We propose that the recruitment of a single expression site and the concomitant exclusion of inactive loci from a discrete transcriptional body define the mechanism responsible for VSG mono-allelic expression...
- Phylogenetic analyses of diplomonad genes reveal frequent lateral gene transfers affecting eukaryotesJan O Andersson
The Canadian Institute for Advanced Research, Program in Evolutionary Biology, Department of Biochemistry, Dalhousie University, B3H 4H7, Halifax, Nova Scotia, Canada
Curr Biol 13:94-104. 2003..Here, we perform a systematic analysis to investigate the impact of LGT on the evolution of diplomonads, a group of anaerobic protists...
- Global trends of whole-genome duplications revealed by the ciliate Paramecium tetraureliaJean Marc Aury
Genoscope and CNRS UMR 8030, 2 rue Gaston Crémieux CP5706, 91057 Evry, France
Nature 444:171-8. 2006..The conclusion of this analysis is that many genes are maintained after whole-genome duplication not because of functional innovation but because of gene dosage constraints...
- Discovery of gene function by expression profiling of the malaria parasite life cycleKarine G Le Roch
Department of Cell Biology ICND202, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
Science 301:1503-8. 2003..Genes with highly correlated levels and temporal patterns of expression were often involved in similar functions or cellular processes...
- The intestinal protozoan parasite Entamoeba histolytica contains 20 cysteine protease genes, of which only a small subset is expressed during in vitro cultivationIris Bruchhaus
Bernhard Nocht Institute for Tropical Medicine, 20359 Hamburg, Germany
Eukaryot Cell 2:501-9. 2003..histolytica or E. dispar trophozoites during in vitro cultivation. Therefore, it is likely that at least some of these enzymes are required for infection of the human host and/or for completion of the parasite life cycle...
- A secreted serine-threonine kinase determines virulence in the eukaryotic pathogen Toxoplasma gondiiS Taylor
Department of Molecular Microbiology, Washington University School of Medicine, St Louis, MO 63130, USA
Science 314:1776-80. 2006..These attributes of ROP18 required kinase activity, which revealed that secretion of effectors is a major component of parasite virulence...
- Trypanosoma cruzi surface mucins: host-dependent coat diversityCarlos A Buscaglia
Instituto de Investigaciones Biotecnologicas Instituto Tecnologico de Chascomus IIB INTECH, Universidad Nacional de General San Martn CONICET, San Martin 1650, Buenos Aires, Argentina
Nat Rev Microbiol 4:229-36. 2006..Here, we discuss the expression profiling of T. cruzi mucins, the mechanisms leading to the acquisition of mucin diversity and the possible consequences of a mosaic surface coat in the interplay between parasite and host...
- Analysis of a piwi-related gene implicates small RNAs in genome rearrangement in tetrahymenaKazufumi Mochizuki
Department of Biology, University of Rochester, Rochester, NY 14627, USA
Cell 110:689-99. 2002..We propose that these small RNAs function to specify sequences to be eliminated by a mechanism similar to RNA-mediated gene silencing...
- Improvements in transfection efficiency and tests of RNA interference (RNAi) approaches in the protozoan parasite LeishmaniaKelly A Robinson
Department of Molecular Microbiology, Washington University Medical School, 660 S Euclid Avenue, St Louis, MO 63110, USA
Mol Biochem Parasitol 128:217-28. 2003..The implications to parasite biology, gene amplification, and genetic analysis are discussed...
- Association of severe noncerebral Plasmodium falciparum malaria in Brazil with expressed PfEMP1 DBL1 alpha sequences lacking cysteine residuesKarin Kirchgatter
Departmento de Parasitologia, Instituto de Ciencias Biomedicas, Universidade de Sao Paulo, Brazil
Mol Med 8:16-23. 2002..We analyzed parasites of Brazilian patients with severe malaria to determine whether there were particular DBL1 alpha var sequences predominantly expressed in such patients...
- Telomeric heterochromatin propagation and histone acetylation control mutually exclusive expression of antigenic variation genes in malaria parasitesLucio H Freitas-Junior
Institut Pasteur, Biology of Host Parasite Interactions Unit CNRS URA2581, 25, rue du Dr Roux, 75724 Paris, France
Cell 121:25-36. 2005..Our data demonstrate that mutually exclusive transcription of var genes is linked to the dynamic remodeling of chromatin...
- Germ line transcripts are processed by a Dicer-like protein that is essential for developmentally programmed genome rearrangements of Tetrahymena thermophilaColin D Malone
Biology Department, Washington University, St Louis, MO 63130, USA
Mol Cell Biol 25:9151-64. 2005....
- Targeting malaria virulence and remodeling proteins to the host erythrocyteMatthias Marti
Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria 3050, Australia
Science 306:1930-3. 2004..The conservation of this signal across Plasmodium species has implications for the development of new antimalarials...
- A host-targeting signal in virulence proteins reveals a secretome in malarial infectionN Luisa Hiller
Departments of Pathology and Microbiology Immunology, Feinberg School of Medicine, Northwestern University, 303 East Chicago Avenue, Chicago, IL 60611, USA
Science 306:1934-7. 2004..Erythrocyte modification by the parasite may involve plasmodial heat shock proteins and be vastly more complex than hitherto realized...
- Reticulocyte-binding protein homologue 1 is required for sialic acid-dependent invasion into human erythrocytes by Plasmodium falciparumTony Triglia
The Walter and Eliza Hall Institute of Medical Research, Melbourne 3050, Australia
Mol Microbiol 55:162-74. 2005..falciparum parasites to utilize alternative receptors on the erythrocyte surface for evasion of receptor polymorphisms and the host immune system...
- A study of genetic diversity in the gene encoding the circumsporozoite protein (CSP) of Plasmodium falciparum from different transmission areas--XVI. Asembo Bay Cohort ProjectAnanias A Escalante
Department of Biology, Emory University, Atlanta, GA, USA
Mol Biochem Parasitol 125:83-90. 2002..We conclude that it is inappropriate to use linkage-recombination patterns on genes under positive selection for assessing the structure of parasite populations...
- Horizontal gene transfer from Bacteria to rumen Ciliates indicates adaptation to their anaerobic, carbohydrates-rich environmentGuenola Ricard
Center for Molecular and Biomolecular Informatics, Nijmegen Center for Molecular Life Sciences, Radboud University Nijmegen Medical Centre, Toernooiveld 1, 6525 ED Nijmegen, The Netherlands
BMC Genomics 7:22. 2006....
- Comparative analysis of apicomplexa and genomic diversity in eukaryotesThomas J Templeton
Department of Microbiology and Immunology, Weill Medical College and the Program in Immunology and Microbial Pathogenesis, Weill Graduate School of Medical Sciences of Cornell University, New York, New York 10021, USA
Genome Res 14:1686-95. 2004..We also describe additional molecular characteristics distinguishing Apicomplexa from other eukaryotes for which complete genome sequences are available...
- Polymorphic secreted kinases are key virulence factors in toxoplasmosisJ P J Saeij
Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA
Science 314:1780-3. 2006..Both are hypervariable rhoptry proteins that are secreted into the host cell upon invasion. These results suggest that secreted kinases unique to the Apicomplexa are crucial in the host-pathogen interaction...
- Comparative genomics of trypanosomatid parasitic protozoaNajib M El-Sayed
Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA
Science 309:404-9. 2005..Contrary to recent reports, our analyses reveal no evidence that these species are descended from an ancestor that contained a photosynthetic endosymbiont...
- Expression and function of pvcrt-o, a Plasmodium vivax ortholog of pfcrt, in Plasmodium falciparum and Dictyostelium discoideumJuliana Martha Sá
Departamento de Parasitologia, Instituto de Ciencias Biomedicas, Universidade de Sao Paulo, Av Lineu Prestes 1374, Sao Paulo 05508 900, SP, Brazil
Mol Biochem Parasitol 150:219-28. 2006..Our data support an effect of PvCRT-o on chloroquine transport and/or accumulation by P. vivax, independent of the K76T amino acid substitution...
- Extragenic bypass suppressors of mutations in the essential gene BLD2 promote assembly of basal bodies with abnormal microtubules in Chlamydomonas reinhardtiiA M Preble
Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, Colorado 80309-0347, USA
Genetics 157:163-81. 2001..It is likely that BLD2 is an essential gene that is needed for basal body assembly and function...
- Active transcription is required for maintenance of epigenetic memory in the malaria parasite Plasmodium falciparumRon Dzikowski
Department of Parasitology, The Kuvin Center for the Study of Infectious and Tropical Diseases, The Institute for Medical Research, The Hebrew University Hadassah Medical School, Jerusalem 91120, Israel
J Mol Biol 382:288-97. 2008....
- Protection against Leishmania donovani infection by DNA vaccination: increased DNA vaccination efficiency through inhibiting the cellular p53 responseA Ghosh
Department of Microbiology and Immunology, McGill University, 3775 University Street, Montreal, H3A 2B4, Quebec, Canada
Vaccine 19:3169-78. 2001..These results have important implications for DNA vaccination against leishmaniasis and potentially against other infectious diseases...
- A phylogenetic comparison of gene trees constructed from plastid, mitochondrial and genomic DNA of Plasmodium speciesD Rathore
Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 4 Center Drive MSC 0425, Bethesda, MD 20892-0425, USA
Mol Biochem Parasitol 114:89-94. 2001..falciparum...
- Biology of Giardia lambliaR D Adam
Department of Medicine, University of Arizona College of Medicine, 1501N Campbell, Tucson, AZ 85724 5049, USA
Clin Microbiol Rev 14:447-75. 2001..The Giardia genome project promises to greatly increase our understanding of this interesting and enigmatic organism...
- A cluster of ring stage-specific genes linked to a locus implicated in cytoadherence in Plasmodium falciparum codes for PEXEL-negative and PEXEL-positive proteins exported into the host cellTobias Spielmann
Infectious Diseases and Immunology Division, Queensland Institute of Medical Research, PO Royal Brisbane Hospital, Herston QLD 4029, Australia
Mol Biol Cell 17:3613-24. 2006..Biochemical and photobleaching studies using these proteins reveal new properties of the parasite-induced membrane compartments in the host cell. This has important implications for the biogenesis and connectivity of these structures...
- Large-scale intron conservation and order-of-magnitude variation in intron loss/gain rates in apicomplexan evolutionScott William Roy
Allan Wilson Centre for Molecular Ecology and Evolution, Massey University, Palmerston North, New Zealand
Genome Res 16:1270-5. 2006..We suggest that intron loss/gain in some eukaryotic lineages may be concentrated in relatively short episodes coincident with occasional TE invasions...
- Dinoflagellate expressed sequence tag data indicate massive transfer of chloroplast genes to the nuclear genomeTsvetan R Bachvaroff
Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD 20742, USA
Protist 155:65-78. 2004..A preliminary report of these data was presented at the Botany 2002 meeting in Madison, WI...
- Antigenic variation in Plasmodium falciparumArtur Scherf
Biology of Host Parasite Interactions Unit, CNRS URA2581, Institut Pasteur 75724 Paris, France
Annu Rev Microbiol 62:445-70. 2008..In addition, controversies surrounding var genetic elements in antigenic variation are discussed...
- The genome of the simian and human malaria parasite Plasmodium knowlesiA Pain
Wellcome Trust Sanger Institute, Genome Campus, Hinxton, Cambridgeshire CB10 1SA, UK
Nature 455:799-803. 2008..One of these families, the KIRs, contains sequences that collectively match over one-half of the host CD99 extracellular domain, which may represent an unusual form of molecular mimicry...
- Intercontinental spread of pyrimethamine-resistant malariaCally Roper
London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK
Science 305:1124. 2004..The resistance alleles carried by these migrants are now spreading across Africa at an alarming rate, signaling the end of affordable malaria treatment and presenting sub-Saharan Africa with a public health crisis...
- Temporal order of RNA-processing reactions in trypanosomes: rapid trans splicing precedes polyadenylation of newly synthesized tubulin transcriptsE Ullu
Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut 06510 8056
Mol Cell Biol 13:720-5. 1993..Thus, trans splicing is cotranscriptional. In addition, we show that trans splicing precedes polyadenylation in the processing of trypanosome tubulin pre-mRNAs...
- Inhibitors of the nonmevalonate pathway of isoprenoid biosynthesis as antimalarial drugsH Jomaa
Institute of Biochemistry, Academic Hospital Centre, Justus Liebig University, Friedrichstrasse 24, D 35392 Giessen, Germany
Science 285:1573-6. 1999..Both drugs suppressed the in vitro growth of multidrug-resistant P. falciparum strains. After therapy with these drugs, mice infected with the rodent malaria parasite P. vinckei were cured...
- Regulation of apicomplexan actin-based motilityJake Baum
Division of Infection and Immunity, The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria 3050, Australia
Nat Rev Microbiol 4:621-8. 2006....
- Plasmodium vivax and the importance of the subtelomeric multigene vir superfamilyCarmen Fernandez-Becerra
Barcelona Centre for International Health Research, Hospital Clinic IDIBAPS, Universitat de Barcelona, Rosello 132, 08036 Barcelona, Spain
Trends Parasitol 25:44-51. 2009..vivax and from data gathered from experimental infections in reticulocyte-prone non-lethal malaria parasites and natural P. vivax infections...
- Multi-character population study of the vir subtelomeric multigene superfamily of Plasmodium vivax, a major human malaria parasiteEmilio F Merino
Departamento de Parasitologia, Instituto de Ciencias Biomedicas, Universidade de São, Paulo, Av Lineu Prestes 1374, Sao Paulo, SP 05508 900, Brazil
Mol Biochem Parasitol 149:10-6. 2006..These results suggest that VIR proteins can have different subcellular localizations and functions. This is the first study on a population level of the P. vivax vir subtelomeric multigene superfamily...
- Telomere and subtelomere of Trypanosoma cruzi chromosomes are enriched in (pseudo)genes of retrotransposon hot spot and trans-sialidase-like gene families: the origins of T. cruzi telomeresDong Kim
Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, UNIFESP, Rua Botucatu, 862, CEP 04023 062, S Paulo, Brazil
Gene 346:153-61. 2005..cruzi. Finally, a mechanism for generation of T. cruzi telomere by chromosome breakage and telomere healing is proposed...
- Plasmodium falciparum associated with severe childhood malaria preferentially expresses PfEMP1 encoded by group A var genesAnja T R Jensen
Department of Medical Microbiology and Immunology, The Panum Institute, Building 24 2, Blegdamsvej 3, 2200 Copenhagen, Denmark
J Exp Med 199:1179-90. 2004..The proteins encoded by Group A var genes, such as PFD1235w/MAL7P1.1, appear to be involved in the pathogenesis of severe disease and are thus attractive candidates for a vaccine against life-threatening P. falciparum malaria...
- Evidence for selection for the tyrosine-86 allele of the pfmdr 1 gene of Plasmodium falciparum by chloroquine and amodiaquineM T Duraisingh
Department of Medical Parasitology, London School of Hygiene and Tropical Medicine, UK
Parasitology 114:205-11. 1997..The samples were genotyped at 3 unlinked polymorphic loci. These analyses suggested that most parasites observed at day 7 were probably recrudescences whereas most of those at day 28 were reinfections...
- Nucleotide sequences provide evidence of genetic exchange among distantly related lineages of Trypanosoma cruziC A Machado
Department of Ecology and Evolutionary Biology, University of California, Irvine, CA 92697, USA
Proc Natl Acad Sci U S A 98:7396-401. 2001..We estimate that the major extant lineages of T. cruzi have diverged during the Miocene or early Pliocene (3-16 million years ago)...
- The genome of the kinetoplastid parasite, Leishmania majorAlasdair C Ivens
Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire CB10 1SA, UK
Science 309:436-42. 2005..Abundant RNA-binding proteins are encoded in the Tritryp genomes, consistent with active posttranscriptional regulation of gene expression...
- Heterochromatin silencing and locus repositioning linked to regulation of virulence genes in Plasmodium falciparumManoj T Duraisingh
The Walter and Eliza Hall Institute of Medical Research, Melbourne 3050, Australia
Cell 121:13-24. 2005..Together, this implies that locus repositioning and heterochromatic silencing play important roles in the epigenetic regulation of virulence genes in P. falciparum...
- Detecting selection using a single genome sequence of M. tuberculosis and P. falciparumJoshua B Plotkin
Harvard Society of Fellows and Bauer Center for Genomics Research, 7 Divinity Avenue, Cambridge, Massachusetts 02138, USA
Nature 428:942-5. 2004..Our method of estimating selective pressures requires far fewer data than comparative sequence analysis, and it measures selection across an entire genome; the method can readily be applied to a large range of sequenced organisms...
- PiggyMac, a domesticated piggyBac transposase involved in programmed genome rearrangements in the ciliate Paramecium tetraureliaCéline Baudry
CNRS FRE 3144, Centre de Genetique Moleculaire, Gif sur Yvette F 91198 Cedex, France
Genes Dev 23:2478-83. 2009..This is the first in vivo evidence suggesting an essential endonucleolytic function of a domesticated piggyBac transposase...
- The SRS superfamily of Toxoplasma surface proteinsCalvin Jung
Infectious Diseases, Department of Medicine, University of British Columbia, D459 HP East, 2733 Heather Street, Vancouver, BC, Canada V5Z 3J5
Int J Parasitol 34:285-96. 2004..Orthologous genes are restricted to tissue-dwelling coccidia (Neospora, Sarcocystis) with no related sequences present in other more distant apicomplexa such as Eimeria, Cryptosporidia, and Plasmodium spp...
- Differential var gene transcription in Plasmodium falciparum isolates from patients with cerebral malaria compared to hyperparasitaemiaHelen M Kyriacou
Institute of Immunology and Infection Research, School of Biological Sciences, University of Edinburgh, Edinburgh EH9 3JT, UK
Mol Biochem Parasitol 150:211-8. 2006..These results suggest that var genes with DBLalpha1-like domains (Group A or B/A) may be implicated in the pathogenesis of cerebral malaria, while var genes with DBLalpha0-like domains promote less virulent malaria infections...
- Gene amplification of the multidrug resistance 1 gene of Plasmodium vivax isolates from Thailand, Laos, and MyanmarMallika Imwong
Department of Clinical Tropical Medicine, Faculty of Tropical Medicine, Mahidol University, 420 6 Rajvithi Rd, Bangkok 10400, Thailand
Antimicrob Agents Chemother 52:2657-9. 2008..02). Five coding mutations in pvmdr1, independent of gene amplification, were also found...
- Identification of a sporozoite-specific member of the Toxoplasma SAG superfamily via genetic complementationJay R Radke
Department of Veterinary Molecular Biology, Montana State University, Bozeman, Montana 59717 3610, USA
Mol Microbiol 52:93-105. 2004..Expression of SporoSAG in tachyzoites conferred enhanced invasion on transgenic parasites suggesting a role for this protein in oocyst/sporozoite transmission to susceptible hosts...
- Global genetic diversity and evolution of var genes associated with placental and severe childhood malariaAdama R Trimnell
Seattle Biomedical Research Institute, Seattle, WA, USA
Mol Biochem Parasitol 148:169-80. 2006..These studies aid our understanding of the evolutionary mechanisms that shape var diversity and will be important to the development of vaccines against pregnancy associated malaria and severe malaria...
- Microarray analyses of gene expression during the Tetrahymena thermophila life cycleWei Miao
Department of Biology, University of Rochester, Rochester, New York, USA
PLoS ONE 4:e4429. 2009..The model eukaryote, Tetrahymena thermophila, is the first ciliated protozoan whose genome has been sequenced, enabling genome-wide analysis of gene expression...
- KIN241: a gene involved in cell morphogenesis in Paramecium tetraurelia reveals a novel protein family of cyclophilin-RNA interacting proteins (CRIPs) conserved from fission yeast to manA Krzywicka
, Centre National de la Recherche Scientifique, 91198 Gif-sur-Yvette Cedex, France
Mol Microbiol 42:257-67. 2001..No Kin241-1 protein is present in mutant cells, suggesting that the C-terminal serine-rich region is responsible for protein stability...
- Antigenic variation in Babesia bovis occurs through segmental gene conversion of the ves multigene family, within a bidirectional locus of active transcriptionBasima Al-Khedery
Department of Pathobiology, University of Florida, Gainesville, 32611, USA
Mol Microbiol 59:402-14. 2006..These results also suggest the possibility of epigenetic regulation through in situ switching among gene loci, further expanding the potential repertoire of variant proteins...
- A spliceosomal intron in Giardia lambliaJulie E J Nixon
Department of Immunology and Infectious Diseases, Harvard School of Public Health, 665 Huntington Avenue, Boston, MA 02115 6018, USA
Proc Natl Acad Sci U S A 99:3701-5. 2002..If introns were added during eukaryotic evolution (the introns-late hypothesis), then these results push back the date of this event before the branching of G. lamblia...
- Differential gene expression in Entamoeba histolytica isolated from amoebic liver abscessIris Bruchhaus
Bernhard Nocht Institute for Tropical Medicine, Bernhard Nocht Str 74, 20359 Hamburg, Germany
Mol Microbiol 44:1063-72. 2002....
- Genome sequence of Theileria parva, a bovine pathogen that transforms lymphocytesMalcolm J Gardner
Institute for Genomic Research TIGR, 9712 Medical Center Drive, Rockville, MD 20850, USA
Science 309:134-7. 2005..Several biosynthetic pathways are incomplete or absent, suggesting substantial metabolic dependence on the host cell. One protein family that may generate parasite antigenic diversity is not telomere-associated...
- Genes induced by a high-oxygen environment in Entamoeba histolyticaMd Ali Akbar
Department of Microbiology, National Institute of Cholera and Enteric Diseases, P 33 C I T Road, Scheme XM, Kolkata 700 010, West Bengal, India
Mol Biochem Parasitol 133:187-96. 2004..signal transduction, tissue destruction, and oxidative stress management, including enhanced expression of a 29-kDa Eh29, suggest that this organism has several protective mechanisms to deal with oxidative stress during invasion...
- Direct visualisation of individual gene organisation in Trypanosoma brucei by high-resolution in situ hybridisationK Ersfeld
School of Biological Sciences, 2 205 Stopford Building, University of Manchester, Manchester M13 9PT, UK
Chromosoma 107:237-40. 1998..We demonstrated the repetitive nature of the tubulin gene cluster, which consists of up to 19 alpha- and beta-tubulin genes arranged in tandem repeats...
- A sub-family of common and highly conserved Plasmodium falciparum var genesAli Salanti
Centre for Medical Parasitology at Institute for Medical Microbiology and Immunology, University of Copenhagen and Department of Infectious Diseases, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark
Mol Biochem Parasitol 122:111-5. 2002
- Identification and molecular characterisation of DNA damaging agent induced expression of Plasmodium falciparum recombination protein PfRad51Mrinal Kanti Bhattacharyya
Johns Hopkins University Bloomberg School of Public Health, 615 N Wolfe Street, Baltimore, MD 21205, USA
Int J Parasitol 33:1385-92. 2003..PfRad51 is the first apicomplexan gene identified that codes for a recombination protein, and it offers an excellent model for studying DNA damage inducible gene expression in such parasites...
- Rapid evolution of an erythrocyte invasion gene family: the Plasmodium reichenowi Reticulocyte Binding Like (RBL) genesJulian C Rayner
Division of Parasitic Diseases, Centers for Disease Control and Prevention, National Center for Infectious Diseases, Atlanta, GA 30341, USA
Mol Biochem Parasitol 133:287-96. 2004..The RBL super-family, therefore, appears to be surprisingly dynamic and divergent, implying that it is involved in species-specific aspects of erythrocyte recognition and invasion...
- Organization, transcription, and expression of rhoptry associated protein genes in the Babesia bigemina rap-1 locusCarlos E Suarez
Program in Vector Borne Diseases, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164 7040, USA
Mol Biochem Parasitol 127:101-12. 2003..Immunoblot analysis of merozoites revealed the expected RAP-1a expression but failed to detect expressed RAP-1b and RAP-1c, indicating that expression of the rap-1 genes is regulated both at the transcriptional and translational levels...
- Dynamic RNA profiling in Plasmodium falciparum synchronized blood stages exposed to lethal doses of artesunateOnguma Natalang
Institut Pasteur, Unité d Immunologie Moléculaire des Parasites, CNRS URA 2581, 28 rue du Docteur Roux, F 75724, Paris, Cedex 15, France
BMC Genomics 9:388. 2008..However, few studies to date have used this powerful approach to explore transcriptome alterations of P. falciparum parasites exposed to antimalarial drugs...
- Structure and content of the Entamoeba histolytica genomeC G Clark
Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London WC1E 7HT, UK
Adv Parasitol 65:51-190. 2007..In this chapter we summarise the features of the genome as they are currently understood and provide previously unpublished analyses of many of the genes...
- Presence of multiple mRNA cycling sequence element-binding proteins in Crithidia fasciculataBidyottam Mittra
Molecular Biology Institute and Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, California 90095, USA
J Biol Chem 278:26564-71. 2003..Three polypeptides associated with purified CSBP II show specific binding to the cycling sequence. These proteins may represent a family of sequence-specific RNA-binding proteins involved in post-transcriptional regulation...
- A mitogen-activated protein (MAP) kinase homologue of Leishmania mexicana is essential for parasite survival in the infected hostM Wiese
Max Planck Institut fur Biologie, Abteilung Membranbiochemie, Corrensstrasse 38, D 72076 Tubingen, Germany
EMBO J 17:2619-28. 1998..Therefore, LMPK is a kinase that is essential for the survival of L.mexicana in the infected host by affecting the cell division of the amastigotes...
- Adaptations of Trypanosoma brucei to gradual loss of kinetoplast DNA: Trypanosoma equiperdum and Trypanosoma evansi are petite mutants of T. bruceiDe Hua Lai
Biology Centre, Institute of Parasitology, Czech Academy of Sciences, 37005 Ceske Budejovice, Czech Republic
Proc Natl Acad Sci U S A 105:1999-2004. 2008..They should be considered two subspecies, respectively T. brucei equiperdum and T. brucei evansi, which spontaneously arose recently. Dk/Ak trypanosomes may potentially emerge repeatedly from T. brucei...
- An EBA175 homologue which is transcribed but not translated in erythrocytic stages of Plasmodium falciparumT Triglia
The Walter and Eliza Hall Institute of Medical Research, Royal Melbourne Hospital, Melbourne, Victoria 3050, Australia
Mol Biochem Parasitol 116:55-63. 2001..Additionally, it was possible to disrupt the Psi EBA165 gene without affecting the parasite's ability to invade and grow in erythrocytes. These results suggest that the Psi EBA165 gene is a transcribed pseudogene...
- Recent origin of Plasmodium falciparum from a single progenitorS K Volkman
The Harvard-Oxford Malaria Genome Diversity Project, Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, MA 02115, USA
Science 293:482-4. 2001..These data establish the recent origin of P. falciparum and further provide an explanation for the abundant diversity observed in antigen and other selected genes...
- The transcription machinery and the molecular toolbox to control gene expression in Toxoplasma gondii and other protozoan parasitesMarkus Meissner
Hygieneinstitut, Abteilung Parasitologie, Universitatsklinikum Heidelberg, Im Neuenheimer Feld 324, D 69120 Heidelberg, Germany
Microbes Infect 7:1376-84. 2005..These findings have some direct implications on development of tools...
- Mitochondrial remnant organelles of Giardia function in iron-sulphur protein maturationJorge Tovar
School of Biological Sciences, Royal Holloway, University of London, Egham, Surrey TW20 0EX, UK
Nature 426:172-6. 2003..Our results indicate that Giardia is not primitively amitochondrial and that it has retained a functional organelle derived from the original mitochondrial endosymbiont...
- Targeting of a tetracycline-inducible expression system to the transcriptionally silent minichromosomes of Trypanosoma bruceiBill Wickstead
School of Biological Sciences, University of Manchester, 2 205 Stopford Building, Oxford Road, Manchester M13 9PT, UK
Mol Biochem Parasitol 125:211-6. 2002
- Genetic structure of the mating-type locus of Chlamydomonas reinhardtiiPatrick J Ferris
Department of Biology, Washington University, St Louis, Missouri 63130, USA
Genetics 160:181-200. 2002..This report extends our original conclusion that the MT locus has incurred high levels of mutational change...
- A well-conserved Plasmodium falciparum var gene shows an unusual stage-specific transcript patternSue A Kyes
Molecular Parasitology Group, Weatherall Institute of Molecular Medicine, Headington, Oxford OX3 9DS, UK
Mol Microbiol 48:1339-48. 2003..Although varCSA has been shown to encode a chondroitin sulphate A (CSA)-binding PfEMP1, we find that the presence of full-length varCSA transcripts does not correlate with the CSA-binding phenotype...
- Tetrahymena histone acetyltransferase A: a homolog to yeast Gcn5p linking histone acetylation to gene activationJ E Brownell
Department of Biology, University of Rochester, New York, 14627, USA
Cell 84:843-51. 1996..These findings shed light on the biochemical function of the evolutionarily conserved Gcn5p-Ada complex, directly linking histone acetylation to gene activation, and indicate that histone acetylation is a targeted phenomenon...
- Expression and function of surface proteins in Trypanosoma bruceiE Pays
Department of Molecular Biology, University of Brussels 67, Rhode St Genese, Belgium
Mol Biochem Parasitol 91:3-36. 1998
- Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13N Hall
The Wellcome Trust Sanger Institute, The Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
Nature 419:527-31. 2002..We identify a highly conserved sequence element found in the intergenic region of internal var genes that is not associated with their telomeric counterparts...
- Short hairpin RNA-mediated knockdown of protein expression in Entamoeba histolyticaAlicia S Linford
Department of Microbiology, University of Virginia, Charlottesville, Virginia, USA
BMC Microbiol 9:38. 2009..We chose to test the use of RNA interference to knock down gene expression in Entamoeba histolytica...
- Multifarious transcriptional regulation of adhesion protein gene ap65-1 by a novel Myb1 protein in the protozoan parasite Trichomonas vaginalisShiou Jeng Ong
Division of Infectious Diseases, Institute of Biomedical Sciences, Academia Sinica, Taipei 11529, Taiwan
Eukaryot Cell 5:391-9. 2006..Together, these observations suggest that Myb1 critically regulates multifarious ap65-1 transcription, possibly via differential selection of multiple promoter sites upon environmental changes...
- Genetic and biochemical analysis of development in Toxoplasma gondiiJ C Boothroyd
Department of Microbiology and Immunology, Stanford University School of Medicine, CA 94305 5402, USA
Philos Trans R Soc Lond B Biol Sci 352:1347-54. 1997..Chief among the techniques used for these studies are genetics and molecular genetics. Recent progress in these areas is described...
- Mosaic organization and heterogeneity in frequency of allelic recombination of the Plasmodium vivax merozoite surface protein-1 locusChaturong Putaporntip
Laboratory of Biology and Department of Mathematics, Osaka Institute of Technology, Osaka 535 8585, Japan
Proc Natl Acad Sci U S A 99:16348-53. 2002..Meanwhile, highly reduced nucleotide diversity was noted in a region encoding the 19-kDa C-terminal epidermal growth factor-like domain of merozoite surface protein-1, a vaccine candidate...
- Complete genome sequence of the apicomplexan, Cryptosporidium parvumMitchell S Abrahamsen
Department of Veterinary and Biomedical Science, College of Veterinary Medicine, University of Minnesota, St Paul, MN 55108, USA
Science 304:441-5. 2004..Elucidation of the core metabolism, including enzymes with high similarities to bacterial and plant counterparts, opens new avenues for drug development...
- A novel Plasmodium falciparum ring stage protein, REX, is located in Maurer's cleftsPaula L Hawthorne
University of Queensland, Herston, 4006 QLD, Australia
Mol Biochem Parasitol 136:181-9. 2004..REX persists in Maurer's clefts throughout the infection of the erythrocyte, where it may play a role in the biogenesis and/or function of this organelle...
- The ves multigene family of B. bovis encodes components of rapid antigenic variation at the infected erythrocyte surfaceD R Allred
Department of Pathobiology, University of Florida, Gainesville 32611, USA
Mol Cell 5:153-62. 2000..Variation appears to involve creation and modification or loss of a novel, transcribed copy of the gene...
- ParameciumDB: a community resource that integrates the Paramecium tetraurelia genome sequence with genetic dataOlivier Arnaiz
Centre de Genetique Moleculaire, CNRS, 91198 Gif sur Yvette, France
Nucleic Acids Res 35:D439-44. 2007..Future ParameciumDB development plans include coordination of human curation of the near 40 000 gene models by members of the research community...
- Sampling gene diversity across the supergroup Amoebozoa: large EST data sets from Acanthamoeba castellanii, Hartmannella vermiformis, Physarum polycephalum, Hyperamoeba dachnaya and Hyperamoeba spRussell F Watkins
Department of Biochemistry and Molecular Biology, Dalhousie University, Halifax, Nova Scotia, Canada B3H 1X5
Protist 159:269-81. 2008....
- A rhoptry-protein-associated mechanism of clonal phenotypic variation in rodent malariaP R Preiser
Division of Parasitology, National Institute for Medical Research, London, UK
Nature 398:618-22. 1999..This phenomenon is genetically and functionally distinct from classical antigenic variation, which is mediated by the var multigene family of P. falciparum...
- Primary structure and expression of the dihydropteroate synthetase gene of Plasmodium falciparumT Triglia
Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia
Proc Natl Acad Sci U S A 91:7149-53. 1994..We have determined the sequence of the DHPS portion of the gene from sulfadoxine-sensitive and -resistant P. falciparum clones and identified sequence differences that may have a role in sulfone/sulfonamide resistance...
- YakA, a protein kinase required for the transition from growth to development in DictyosteliumG M Souza
Verna and Marrs McLean Department of Biochemistry, Baylor College of Medicine, Houston, Texas 77030, USA
Development 125:2291-302. 1998..YakA appears to regulate the transition from growth to development in Dictyostelium...
- Diverse expression patterns of subgroups of the rif multigene family during Plasmodium falciparum gametocytogenesisMichaela Petter
Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany
PLoS ONE 3:e3779. 2008..falciparum genome, such as the PfEMP1, STEVOR and RIFIN proteins. While expression of the former two families in sexual stages has been investigated earlier, we report here RIFIN expression during gametocytogenesis...
- HYPOTRICH GENOME IN DEVELOPMENT AND EVOLUTIONDavid Prescott; Fiscal Year: 2001..The experiments deal with the general problem of how an organism can manipulate its own DNA. ..
- Translational Control in Plasmodium Sexual DevelopmentLiwang Cui; Fiscal Year: 2005....
- DRUG RESISTANCE AND PTERIDINE METABOLISM IN LEISHMANIAStephen M Beverley; Fiscal Year: 2010..These studies have yielded new opportunities for chemotherapeutic attack and new directions for future study of pteridine metabolism and biology. [unreadable] ..
- Plasmodium vivax:Proteomic Analysis of Asexual and Sporozoite StagesLiwang Cui; Fiscal Year: 2007..Its recent resurgence in prevalence and emergence of drug- resistant parasite strains have raised a great concern for the future control of malaria. [unreadable] [unreadable] [unreadable]..
- Gene Duplication and Genome EvolutionAustin Hughes; Fiscal Year: 2004..5) Examining the role of transposable elements in duplication of genomic segments by testing for nonrandom association between these elements and putatively duplicated blocks in the yeast genome. ..
- Development of a cell-based method for high-throughput drug screening in malaria Liwang Cui; Fiscal Year: 2008..To facilitate antimalarial drug discovery, we propose to develop a highly sensitive, cell-based, one-step luminescent drug screen assay for high throughput screening of large chemical libraries. [unreadable] [unreadable] [unreadable]..
- A new family of secreted adhesive proteins in PlasmodiumTHOMAS TEMPLETON; Fiscal Year: 2008....
- EVOLUTION OF IMMUNE RECOGNITION AND EVASIONAustin L Hughes; Fiscal Year: 2010..In addition, by increasing our understanding of the population diversity and evolutionary history of human immune system genes, this research will enhance our knowledge of human immune function in health and disease. ..
- Calcium ATPases in Apicomplexan Parasites as Potential DL Sibley; Fiscal Year: 2006..Knowledge gained through these studies may improve the ability to pharmacologically inhibit key calcium regulatory enzymes and hence block motility and invasion by this important group of parasites. ..
- Nucleoside Transporters of Plasmodium falciparumBuddy Ullman; Fiscal Year: 2007..falciparum in order to test whether PfNT1 function is essential to the intact parasite. We will then characterize the resultant transport and growth phenotypes. ..
- R13 travel grant for Polyamine/parasite conference in Portland, ORBuddy Ullman; Fiscal Year: 2006..abstract_text> ..
- GCN5-mediated transcription in AIDS pathogen ToxoplasmaWILLIAM SULLIVAN JR; Fiscal Year: 2007..unreadable] [unreadable] [unreadable]..
- GENETIC ANALYSIS OF PURINE METABOLISM IN LEISHMANIA DONOBuddy Ullman; Fiscal Year: 2007..coli that overexpress hgprt genes, and[unreadable] intact parasites. ..
- Mucosal Immunity to Toxoplasma gondiiL Sibley; Fiscal Year: 2007..unreadable] [unreadable] [unreadable]..
- Role of Cryptosporidium Mucins in Host-parasite interactionsROBERTA O CONNOR; Fiscal Year: 2007..unreadable] [unreadable] [unreadable]..
- GCN5-mediated transcription in AIDS pathogen ToxoplasmaWILLIAM SULLIVAN JR; Fiscal Year: 2008..Thus, our results will fundamentally advance the fields of parasitology and eukaryotic gene expression. ..
- Differentiation in T.cruzi:an emerging AIDS pathogenHuan Huang; Fiscal Year: 2009..To further delineate the components of this pathway, we will identify the TcPKA downstream interacting molecules, using both yeast two-hybrid assays and proteomic approaches. ..
- Drug resistance in Plasmodium falciparumJohanna Daily; Fiscal Year: 2007..abstract_text> ..
- Novel Anti-Malarials by Combinatorial PharmacogenomicsJoseph DeRisi; Fiscal Year: 2006..The compiled relationships from known anti-malarials, novel quinolines, and toxicology will then be used to guide and optimize additional synthesis efforts toward the ultimate goal of producing effective therapeutics. ..
- GENETIC DISSECTION OF TOXOPLASMA GONDIIDavid Roos; Fiscal Year: 2008..In addition to their value for the analysis of folate metabolism and drug resistance, these tools should be broadly applicable to other studies on the biology and biochemistry of Toxoplasma. ..
- APEs as novel drug targets in AIDS opportunistic pathogen ToxoplasmaWILLIAM SULLIVAN JR; Fiscal Year: 2008..Moreover, Toxoplasma can be informative as a model organism to study Plasmodium, the causative agent of malaria. [unreadable] [unreadable] [unreadable] [unreadable]..
- Polyamine Metabolism in LeishmaniaBuddy Ullman; Fiscal Year: 2008..donovani. We propose, therefore, to ascertain whether the disparity in the abilities of ?lmarg L. mexicana and ?ldodc L. donovani to infect mice can be ascribed to species or gene differences. ..
- ZEISS AXIOPLAN 2 IMAGING SYSTEMROBERTA O CONNOR; Fiscal Year: 2005..abstract_text> ..
- MECHANISM-BASED DRUG SELECTION AND DESIGN: NUCLEOTIDE SABuddy Ullman; Fiscal Year: 2003..Concurrent experiments will determine how NTPase functions in AK HGXPRT-, and AT parasites, identify the protein(s) which regulate NTPase enzymatic activity, and evaluate the impact of abrogating NTPase expression on parasite viability. ..
- Gut Epithelial Cell Receptors for C parvum InvasionROBERTA O CONNOR; Fiscal Year: 2005..New England Medical Center, in association with Tufts University and the wider Boston scientific community provides researchers with diverse and excellent scientific resources, and is exceptional environment for a young scientist...
- Confocal Microscope for MicrobiologyL Sibley; Fiscal Year: 2002..This instrument will greatly strengthen the research programs of these highly productive groups by applying high-resolution confocal microscopy to the study of human pathogens...
- GENE DISCOVERY IN TOXOPLASMA GONDIIL Sibley; Fiscal Year: 2003..In combination with the genetic tools available in T. gondii, this will greatly accelerate investigations into the complex developmental biology of apicomplexan parasites. ..
- P. falciparum vaccine: Evaluating candidacy of PfMSP3/PfMSP6 in an endemic settinJulian Rayner; Fiscal Year: 2008..This data will directly impact vaccine design by allowing us to rule specific sub-domains in or out of the ongoing malaria vaccine development process. [unreadable] [unreadable] [unreadable]..
- Conference Proposal to support FASEB Conference on Ciliate Molecular BiologyCarolyn Price; Fiscal Year: 2007..unreadable] [unreadable] [unreadable]..
- MOLECULAR ANALYSIS OF ANTIGENIC VARIATION IN MALARIAMary Galinski; Fiscal Year: 2004....
- Resistance of Malaria Parasites to Artemisinin-Based Combination TherapiesPhilip Rosenthal; Fiscal Year: 2008..abstract_text> ..
- Differentiation and Signaling in ToxoplasmosisKami Kim; Fiscal Year: 2008..These studies will further elucidate cAMP signaling pathways in T. gondii and determine the role of cAMP signaling pathways during T. gondii differentiation into latent bradyzoites. ..
- REGULATION OF DEVELOPMENT BY PHOSPHORELAY SIGNALINGCHARLES SINGLETON; Fiscal Year: 2002..Finally genetic screens for suppressers or synthetic phenotypes will identify new and perhaps novel components of the regulatory pathway, including upstream inputs, phosphorelay regulators, and downstream effectors. ..
- RNA METABOLISM IN TRYPANOSOMESElisabetta Ullu; Fiscal Year: 2009..3. Further characterize the RNAi pathway in terms of its components and how the interaction of TbAGO1 with translating ribosomes is brought about. ..
- Fatty Acid Biosynthesis in Cryptosporidium parvumGuan Zhu; Fiscal Year: 2010..3) To validate that fatty acid metabolic enzymes may serve as rational drug target in Cryptosporidium by discovering inhibitors selectively against, parasite fatty acid metabolic enzymes. ..
- GP46 GENES AND PROTEIN EXPRESSION IN LEISHMANIA CHAGASIJOHN DONELSON; Fiscal Year: 2002....
- Identification of Tetrahymena DNA Rearrangement GenesDOUGLAS LEE CHALKER; Fiscal Year: 2010..abstract_text> ..
- Identification of Transcriptional Regulatory Networks in Entamoeba HistolyticaUpinder Singh; Fiscal Year: 2008..We are interested in understanding the molecular mechanisms that the parasite uses to cause disease. [unreadable] [unreadable] [unreadable]..
- Biosynthesis and Function of Base JRobert S Sabatini; Fiscal Year: 2010..These studies may prove useful in identifying novel approaches to prevention, treatment and diagnosis of the debilitating and deadly diseases caused by these parasites. ..
- CRYPTOSPORIDIUM PARVUM GENOME SEQUENCING PROJECT (MINN)Mitchell Abrahamsen; Fiscal Year: 2002..parvum genome. ..
- Assessing Genetic Diveristy in Entamoeba HistolyticaUpinder Singh; Fiscal Year: 2006..This approach will also provide a number of novel genetic targets for the development of improved epidemiological markers, diagnostic assays, and treatment strategies. ..
- 2006 Biology of Host-Parasite Interactions Gordon Research ConferenceElisabetta Ullu; Fiscal Year: 2006..Host-Cell Invasion (Discussion Leader: Barbara Burleigh: Harvard) Trichomonads and Amoebae (Discussion Leader: Patricia Johnson, UCLA) Parasite Membrane Biology (Discussion Leader: Scott Landfear: University of Oregon) ..
- Falcipain Cysteine Proteases of Malaria ParasitesPhilip Rosenthal; Fiscal Year: 2005..Experiments designed to accomplish these aims should improve our understanding of the biology of malaria parasites and aid in the characterization of falcipains as potential drug targets. ..
- IMPDH as a Drug Target in CryptosporidiumBoris Striepen; Fiscal Year: 2008..This work will provide urgently needed molecular reagents to understand the biology of nutrient uptake and drug resistance in this unique parasite. [unreadable] [unreadable] [unreadable]..
- Role of cysteine proteases in malaria transmissionKIM WILLIAMSON; Fiscal Year: 2006..In addition to increasing our understanding of the genes involved, this information can be used to direct the design of cysteine protease inhibitors that will effectively block malaria transmission. ..
- Chromosome breakage and DNA detection in TetrahymenaMeng Chao Yao; Fiscal Year: 2006..abstract_text> ..
- Purine salvage in Cryptosporidium parvumMitchell Abrahamsen; Fiscal Year: 2005..In addition, these studies demonstrate our ability to use heterologous expression of C. parvum genes in yeast as a means to develop a high-throughput screening assay for the identification of specific inhibitors. ..
- Development of Assay for High Throughput Screen of Parasite Glucose TransporterScott M Landfear; Fiscal Year: 2010..Since glucose is an essential nutrient for these parasites, such compounds would kill the parasite and could act as novel drugs for treatment of the diseases they cause. ..
- VACCINE DEVELOPMENT IN TUBERCULOSISAntonio Campos Neto; Fiscal Year: 2004..In addition to the evaluation of these antigens, we will characterize and clone other vaccine candidates using recently developed approaches for the direct identification of both CD4 and CD8 T cell antigens. ..
- Role of Rac and Rac Effectors in Controlling ChemotaxisRICHARD FIRTEL; Fiscal Year: 2005....
- Ninth International Congress on ToxoplasmosisBoris Striepen; Fiscal Year: 2007..gondii that is not often seen in fields as high paced and[unreadable] competitive and has facilitated rapid and sustained progress in research on this pathogen[unreadable] and related parasites. ..
- Clinical & Molecular Studies of Drug Resistant MalariaPhilip Rosenthal; Fiscal Year: 2008..abstract_text> ..
- TOXOPLASMA DEVELOPMENT AND CELL CYCLEKami Kim; Fiscal Year: 2002..These will be the initial experiments in a long-term effort to define the role of cell cycle regulation in Toxoplasma tachyzoite-bradyzoite differentiation and virulence. ..
- Zinc metalloprotease families in Trypansoma bruceiJOHN DONELSON; Fiscal Year: 2009....
- Gene Structure and Transciptional RegulationRICHARD FIRTEL; Fiscal Year: 2007..abstract_text> ..
- Assembly, Targeting, And Regulation Of Dynein MotorsMary Porter; Fiscal Year: 2004....
- Antigen Detection Assay for TuberculosisAntonio Campos Neto; Fiscal Year: 2006..unreadable] [unreadable] [unreadable]..
- Function & Regulation of two Novel PARPs, tankyrase 1&2Carolyn Price; Fiscal Year: 2008..Aim 4 will investigate the in vivo regulation of tankyrase PARP activity. We will examine the in vivo levels of tankyrase poly(ADP-ribosyl)ation and whether this is regulated by phosphorylation. ..
- SERINE PROTEINASES IN APICOMPLEXAN PARASITESKami Kim; Fiscal Year: 2009..In addition to illuminating the function and biology of T. gondii secretory organelles, these studies will determine whether subtilases are a viable chemotherapeutic target for treatment of human infections with Apicomplexan parasites. ..
- Overexpression of membrane proteins in TetrahymenaTHEODORE CLARK; Fiscal Year: 2006..The technology described here is intended to better understand how these proteins function, leading ultimately to strategies for the treatment of serious disorders ranging from cancer to stoke. [unreadable] [unreadable]..
- Forward Genetics in Tetrahymena: Cloning by ComplementationROBERT COYNE; Fiscal Year: 2008..Numerous other basic studies with far-reaching relevance to human health would be facilitated by the availability of the proposed freely available resource. [unreadable] [unreadable] [unreadable] [unreadable]..
- EXPRESSION OF THE LEISHMANIA TARENTOLAE SL RNA GENEDavid Campbell; Fiscal Year: 2003..These unique features represent potential targets for selective therapeutic intervention. ..
- RNAi in DNA Sequence Elimination in TetrahymenaMartin A Gorovsky; Fiscal Year: 2010..These studies should shed light on other RNAi- like processes that are important for normal development and maintenance of genome stability in multicellular organisms. ..
- "Vivax Malaria Research: 2005 and Beyond"Jane Carlton; Fiscal Year: 2005..vivax with P. falciparum, epidemiology and diversity studies, vectors and transmission dynamics, and a bioinformatics workshop for researchers and students to learn to manipulate Plasmodium sequence data. ..
- Comparative Evolutionary Genomics and Infectious DiseaseJane Carlton; Fiscal Year: 2010....