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The genome sequence of Trypanosoma cruzi, etiologic agent of Chagas diseaseNajib M El-Sayed
Department of Parasite Genomics, Institute for Genomic Research, Rockville, MD 20850, USA
Science 309:409-15. 2005....
Reticulocyte-binding protein homologue 1 is required for sialic acid-dependent invasion into human erythrocytes by Plasmodium falciparumTony Triglia
The Walter and Eliza Hall Institute of Medical Research, Melbourne 3050, Australia
Mol Microbiol 55:162-74. 2005..falciparum parasites to utilize alternative receptors on the erythrocyte surface for evasion of receptor polymorphisms and the host immune system...
Global trends of whole-genome duplications revealed by the ciliate Paramecium tetraureliaJean-Marc Aury
Genoscope and CNRS UMR 8030, , 91057 Evry, France
Nature 444:171-8. 2006..The conclusion of this analysis is that many genes are maintained after whole-genome duplication not because of functional innovation but because of gene dosage constraints...
A phylogenetic comparison of gene trees constructed from plastid, mitochondrial and genomic DNA of Plasmodium speciesD Rathore
Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 4 Center Drive MSC 0425, Bethesda, MD 20892-0425, USA
Mol Biochem Parasitol 114:89-94. 2001..falciparum...
Regulation of apicomplexan actin-based motilityJake Baum
Division of Infection and Immunity, The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria 3050, Australia
Nat Rev Microbiol 4:621-8. 2006....
Inhibitors of the nonmevalonate pathway of isoprenoid biosynthesis as antimalarial drugsH Jomaa
Institute of Biochemistry, Academic Hospital Centre, Justus Liebig University, Friedrichstrasse 24, D 35392 Giessen, Germany
Science 285:1573-6. 1999..Both drugs suppressed the in vitro growth of multidrug-resistant P. falciparum strains. After therapy with these drugs, mice infected with the rodent malaria parasite P. vinckei were cured...
Preferential transcription of conserved rif genes in two phenotypically distinct Plasmodium falciparum parasite linesChristian W Wang
Centre for Medical Parasitology at Department of International Health, Immunology, and Microbiology, University of Copenhagen, Denmark
Int J Parasitol 39:655-64. 2009..This conclusion is experimentally supported by group-specific rif transcription in parasites with different VSA and PfEMP1 expression phenotypes...
Control of gene expression in Plasmodium falciparum - ten years onPaul Horrocks
Institute for Science and Technology in Medicine, Keele University, Staffordshire ST5 5BG, United Kingdom
Mol Biochem Parasitol 164:9-25. 2009..and apicomplexan parasites. Specifically, however, we will address the current polarised debate regarding the level at which control is mediated, and attempt to identify some of the challenges this field faces in the next 10 years...
Differential var gene transcription in Plasmodium falciparum isolates from patients with cerebral malaria compared to hyperparasitaemiaHelen M Kyriacou
Institute of Immunology and Infection Research, School of Biological Sciences, University of Edinburgh, Edinburgh EH9 3JT, UK
Mol Biochem Parasitol 150:211-8. 2006..These results suggest that var genes with DBLalpha1-like domains (Group A or B/A) may be implicated in the pathogenesis of cerebral malaria, while var genes with DBLalpha0-like domains promote less virulent malaria infections...
The SRS superfamily of Toxoplasma surface proteinsCalvin Jung
Infectious Diseases, Department of Medicine, University of British Columbia, D459 HP East, 2733 Heather Street, Vancouver, BC, Canada V5Z 3J5
Int J Parasitol 34:285-96. 2004..Orthologous genes are restricted to tissue-dwelling coccidia (Neospora, Sarcocystis) with no related sequences present in other more distant apicomplexa such as Eimeria, Cryptosporidia, and Plasmodium spp...
P. falciparum rosetting mediated by a parasite-variant erythrocyte membrane protein and complement-receptor 1J A Rowe
Laboratory of Parasitic Diseases, NIAID, NIH, Bethesda, Maryland 20892, USA
Nature 388:292-5. 1997..Thus we describe a new adhesive function for PfEMP1 and raise the possibility that CR1 polymorphisms in Africans that influence the interaction between erythrocytes and PfEMP1 may protect against severe malaria...
Presence of multiple mRNA cycling sequence element-binding proteins in Crithidia fasciculataBidyottam Mittra
Molecular Biology Institute and Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, California 90095, USA
J Biol Chem 278:26564-71. 2003..Three polypeptides associated with purified CSBP II show specific binding to the cycling sequence. These proteins may represent a family of sequence-specific RNA-binding proteins involved in post-transcriptional regulation...
Recent origin of Plasmodium falciparum from a single progenitorS K Volkman
The Harvard-Oxford Malaria Genome Diversity Project, Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, MA 02115, USA
Science 293:482-4. 2001..These data establish the recent origin of P. falciparum and further provide an explanation for the abundant diversity observed in antigen and other selected genes...
An EBA175 homologue which is transcribed but not translated in erythrocytic stages of Plasmodium falciparumT Triglia
The Walter and Eliza Hall Institute of Medical Research, Royal Melbourne Hospital, Melbourne, Victoria 3050, Australia
Mol Biochem Parasitol 116:55-63. 2001..Additionally, it was possible to disrupt the Psi EBA165 gene without affecting the parasite's ability to invade and grow in erythrocytes. These results suggest that the Psi EBA165 gene is a transcribed pseudogene...
Adaptations of Trypanosoma brucei to gradual loss of kinetoplast DNA: Trypanosoma equiperdum and Trypanosoma evansi are petite mutants of T. bruceiDe Hua Lai
Biology Centre, Institute of Parasitology, Czech Academy of Sciences, 37005 Ceske Budejovice, Czech Republic
Proc Natl Acad Sci U S A 105:1999-2004. 2008..They should be considered two subspecies, respectively T. brucei equiperdum and T. brucei evansi, which spontaneously arose recently. Dk/Ak trypanosomes may potentially emerge repeatedly from T. brucei...
A mitogen-activated protein (MAP) kinase homologue of Leishmania mexicana is essential for parasite survival in the infected hostM Wiese
Max Planck Institut fur Biologie, Abteilung Membranbiochemie, Corrensstrasse 38, D 72076 Tubingen, Germany
EMBO J 17:2619-28. 1998..Therefore, LMPK is a kinase that is essential for the survival of L.mexicana in the infected host by affecting the cell division of the amastigotes...
Draft genome sequence of the sexually transmitted pathogen Trichomonas vaginalisJane M Carlton
Institute for Genomic Research, 9712 Medical Research Drive, Rockville, MD 20850, USA
Science 315:207-12. 2007..The genome sequence predicts previously unknown functions for the hydrogenosome, which support a common evolutionary origin of this unusual organelle with mitochondria...
Dinoflagellate expressed sequence tag data indicate massive transfer of chloroplast genes to the nuclear genomeTsvetan R Bachvaroff
Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD 20742, USA
Protist 155:65-78. 2004..A preliminary report of these data was presented at the Botany 2002 meeting in Madison, WI...
Genome sequence of Babesia bovis and comparative analysis of apicomplexan hemoprotozoaKelly A Brayton
Program in Genomics, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, USA
PLoS Pathog 3:1401-13. 2007..The genome sequence provides a greater understanding of B. bovis metabolism and potential avenues for drug therapies and vaccine development...
Sampling gene diversity across the supergroup Amoebozoa: large EST data sets from Acanthamoeba castellanii, Hartmannella vermiformis, Physarum polycephalum, Hyperamoeba dachnaya and Hyperamoeba spRussell F Watkins
Department of Biochemistry and Molecular Biology, Dalhousie University, Halifax, Nova Scotia, Canada B3H 1X5
Protist 159:269-81. 2008....
ParameciumDB: a community resource that integrates the Paramecium tetraurelia genome sequence with genetic dataOlivier Arnaiz
Centre de Genetique Moleculaire, CNRS, 91198 Gif sur Yvette, France
Nucleic Acids Res 35:D439-44. 2007..Future ParameciumDB development plans include coordination of human curation of the near 40 000 gene models by members of the research community...
Dynamic RNA profiling in Plasmodium falciparum synchronized blood stages exposed to lethal doses of artesunateOnguma Natalang
Institut Pasteur, Unité d Immunologie Moléculaire des Parasites, CNRS URA 2581, 28 rue du Docteur Roux, F 75724, Paris, Cedex 15, France
BMC Genomics 9:388. 2008..However, few studies to date have used this powerful approach to explore transcriptome alterations of P. falciparum parasites exposed to antimalarial drugs...
YakA, a protein kinase required for the transition from growth to development in DictyosteliumG M Souza
Verna and Marrs McLean Department of Biochemistry, Baylor College of Medicine, Houston, Texas 77030, USA
Development 125:2291-302. 1998..YakA appears to regulate the transition from growth to development in Dictyostelium...
A rhoptry-protein-associated mechanism of clonal phenotypic variation in rodent malariaP R Preiser
Division of Parasitology, National Institute for Medical Research, London, UK
Nature 398:618-22. 1999..This phenomenon is genetically and functionally distinct from classical antigenic variation, which is mediated by the var multigene family of P. falciparum...
Plasmodium vivax and the importance of the subtelomeric multigene vir superfamilyCarmen Fernandez-Becerra
Barcelona Centre for International Health Research, Hospital Clinic IDIBAPS, Universitat de Barcelona, Rosello 132, 08036 Barcelona, Spain
Trends Parasitol 25:44-51. 2009..vivax and from data gathered from experimental infections in reticulocyte-prone non-lethal malaria parasites and natural P. vivax infections...
Diverse expression patterns of subgroups of the rif multigene family during Plasmodium falciparum gametocytogenesisMichaela Petter
Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany
PLoS ONE 3:e3779. 2008..falciparum genome, such as the PfEMP1, STEVOR and RIFIN proteins. While expression of the former two families in sexual stages has been investigated earlier, we report here RIFIN expression during gametocytogenesis...
Chromatin associated sense and antisense noncoding RNAs are transcribed from the var gene family of virulence genes of the malaria parasite Plasmodium falciparumChristian Epp
Department of Microbiology and Immunology, Weill Medical College of Cornell University, New York, New York 10021, USA
RNA 15:116-27. 2009..These sterile transcripts therefore might act in a manner analogous to noncoding RNAs associated with silent, condensed chromatin found in other eukaryotic systems...
Primary structure and expression of the dihydropteroate synthetase gene of Plasmodium falciparumT Triglia
Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia
Proc Natl Acad Sci U S A 91:7149-53. 1994..We have determined the sequence of the DHPS portion of the gene from sulfadoxine-sensitive and -resistant P. falciparum clones and identified sequence differences that may have a role in sulfone/sulfonamide resistance...
Reverse genetics screen identifies six proteins important for malaria development in the mosquitoAndrea Ecker
Division of Cell and Molecular Biology, Imperial College London, London SW7 2AZ, UK
Mol Microbiol 70:209-20. 2008....
The genome of the simian and human malaria parasite Plasmodium knowlesiA Pain
Wellcome Trust Sanger Institute, Genome Campus, Hinxton, Cambridgeshire CB10 1SA, UK
Nature 455:799-803. 2008..One of these families, the KIRs, contains sequences that collectively match over one-half of the host CD99 extracellular domain, which may represent an unusual form of molecular mimicry...
Characterization of the Crithidia fasciculata mRNA cycling sequence binding proteinsR Mahmood
Molecular Biology Institute and Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, California 90095-1570, USA
Mol Cell Biol 21:4453-9. 2001..CSBPA and CSBPB mRNA and protein levels show little variation throughout the cell cycle, suggesting that additional factors are involved in the cyclic binding to the cycling sequence elements...
Tetrahymena histone acetyltransferase A: a homolog to yeast Gcn5p linking histone acetylation to gene activationJ E Brownell
Department of Biology, University of Rochester, New York, 14627, USA
Cell 84:843-51. 1996..These findings shed light on the biochemical function of the evolutionarily conserved Gcn5p-Ada complex, directly linking histone acetylation to gene activation, and indicate that histone acetylation is a targeted phenomenon...
Identification and molecular characterisation of DNA damaging agent induced expression of Plasmodium falciparum recombination protein PfRad51Mrinal Kanti Bhattacharyya
Johns Hopkins University Bloomberg School of Public Health, 615 N. Wolfe Street, Baltimore, MD 21205, USA
Int J Parasitol 33:1385-92. 2003..PfRad51 is the first apicomplexan gene identified that codes for a recombination protein, and it offers an excellent model for studying DNA damage inducible gene expression in such parasites...
Refined annotation and assembly of the Tetrahymena thermophila genome sequence through EST analysis, comparative genomic hybridization, and targeted gap closureRobert S Coyne
J Craig Venter Institute formerly The Institute for Genomic Research, 9704 Medical Center Dr, Rockville, MD, USA
BMC Genomics 9:562. 2008....
Microarray analyses of gene expression during the Tetrahymena thermophila life cycleWei Miao
Department of Biology, University of Rochester, Rochester, New York, USA
PLoS ONE 4:e4429. 2009..The model eukaryote, Tetrahymena thermophila, is the first ciliated protozoan whose genome has been sequenced, enabling genome-wide analysis of gene expression...
Multifarious transcriptional regulation of adhesion protein gene ap65-1 by a novel Myb1 protein in the protozoan parasite Trichomonas vaginalisShiou-Jeng Ong
Division of Infectious Diseases, Institute of Biomedical Sciences, Academia Sinica, Taipei 11529, Taiwan
Eukaryot Cell 5:391-9. 2006..Together, these observations suggest that Myb1 critically regulates multifarious ap65-1 transcription, possibly via differential selection of multiple promoter sites upon environmental changes...
Protection against Leishmania donovani infection by DNA vaccination: increased DNA vaccination efficiency through inhibiting the cellular p53 responseA Ghosh
Department of Microbiology and Immunology, McGill University, 3775 University Street, Montreal, H3A 2B4, Quebec, Canada
Vaccine 19:3169-78. 2001..These results have important implications for DNA vaccination against leishmaniasis and potentially against other infectious diseases...
Early origin and recent expansion of Plasmodium falciparumDeirdre A Joy
Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892 0425, USA
Science 300:318-21. 2003..However, both the world and some regional populations appear to be older (50,000 to 100,000 years old), suggesting an earlier wave of migration out of Africa, perhaps during the Pleistocene migration of human beings...
Genetic structure of the mating-type locus of Chlamydomonas reinhardtiiPatrick J Ferris
Department of Biology, Washington University, St Louis, Missouri 63130, USA
Genetics 160:181-200. 2002..This report extends our original conclusion that the MT locus has incurred high levels of mutational change...
Rapid evolution of an erythrocyte invasion gene family: the Plasmodium reichenowi Reticulocyte Binding Like (RBL) genesJulian C Rayner
Division of Parasitic Diseases, Centers for Disease Control and Prevention, National Center for Infectious Diseases, Atlanta, GA 30341, USA
Mol Biochem Parasitol 133:287-96. 2004..The RBL super-family, therefore, appears to be surprisingly dynamic and divergent, implying that it is involved in species-specific aspects of erythrocyte recognition and invasion...
Comparative transcriptional and genomic analysis of Plasmodium falciparum field isolatesMargaret J Mackinnon
KEMRI Wellcome Trust Research Programme, Kilifi, Kenya
PLoS Pathog 5:e1000644. 2009..Transcriptional co-regulation of physical clusters of adaptive genes may provide a way for the parasite to readily adapt to its highly heterogeneous and strongly selective environment...
A Plasmodium whole-genome synteny map: indels and synteny breakpoints as foci for species-specific genesTaco W A Kooij
Department of Parasitology, Leiden University Medical Centre, Leiden, The Netherlands
PLoS Pathog 1:e44. 2005..A majority of these genes are expressed in blood stages, suggesting that the vertebrate host exerts a greater selective pressure than the mosquito vector, resulting in the acquisition of diversity...
A var gene promoter controls allelic exclusion of virulence genes in Plasmodium falciparum malariaTill S Voss
The Walter and Eliza Hall Institute of Medical Research, Parkville 3050, Australia
Nature 439:1004-8. 2006..falciparum. Furthermore, the PfEMP1 knockdown parasites obtained in this study will be important tools to increase our understanding of P. falciparum-mediated virulence and immune evasion...
Comparative genomics of trypanosomatid parasitic protozoaNajib M El-Sayed
Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA
Science 309:404-9. 2005..Contrary to recent reports, our analyses reveal no evidence that these species are descended from an ancestor that contained a photosynthetic endosymbiont...
PiggyMac, a domesticated piggyBac transposase involved in programmed genome rearrangements in the ciliate Paramecium tetraureliaCéline Baudry
CNRS FRE 3144, Centre de Genetique Moleculaire, Gif sur Yvette F 91198 Cedex, France
Genes Dev 23:2478-83. 2009..This is the first in vivo evidence suggesting an essential endonucleolytic function of a domesticated piggyBac transposase...
ABC transporters and drug resistance in parasitic protozoaAntonios Klokouzas
Department of Pharmacology, University of Cambridge, Tennis Court Road, Cambridge CB2 1PD, UK
Int J Antimicrob Agents 22:301-17. 2003..Transporters with an established role in drug resistance have been emphasised, but newly discovered transporters with a significant amino acid sequence identity to established ABC drug transporters have also been included...
The genome of the African trypanosome Trypanosoma bruceiMatthew Berriman
Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton CB10 1SA, UK
Science 309:416-22. 2005..brucei and the greatest in L. major. Horizontal transfer of genes of bacterial origin has contributed to some of the metabolic differences in these parasites, and a number of novel potential drug targets have been identified...
Plasmodium falciparum homologue of the genes for Plasmodium vivax and Plasmodium yoelii adhesive proteins, which is transcribed but not translatedH M Taylor
National Institute for Medical Research, Mill Hill, London NW7 1AA, United Kingdom
Infect Immun 69:3635-45. 2001..falciparum (PfRH3) that is transcribed in its entirety, under stage-specific control, with correct splicing of the intron, but appears not to be translated, probably due to two reading frameshifts at the 5' end of the gene...
Nucleotide sequences provide evidence of genetic exchange among distantly related lineages of Trypanosoma cruziC A Machado
Department of Ecology and Evolutionary Biology, University of California, Irvine, CA 92697, USA
Proc Natl Acad Sci U S A 98:7396-401. 2001..We estimate that the major extant lineages of T. cruzi have diverged during the Miocene or early Pliocene (3-16 million years ago)...
Dramatic difference in diversity between Plasmodium falciparum and Plasmodium vivax reticulocyte binding-like genesJulian C Rayner
Division of Parasitic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30329, USA
Am J Trop Med Hyg 72:666-74. 2005..Polymorphisms were frequently clustered, suggesting that different RBL domains may be evolving under different selection and functional pressures...
Genetic structure of Plasmodium falciparum populations in the Brazilian Amazon regionRicardo L D Machado
Malaria Laboratory, Evandro Chagas Institute and National Health Foundation, , Brazil
J Infect Dis 190:1547-55. 2004..falciparum in Brazil are quite independent, with distinct population structures and minimal gene flow, a finding that has implications for strategies to control infection and to contain the spread of drug resistance at a regional level...
Oda5p, a novel axonemal protein required for assembly of the outer dynein arm and an associated adenylate kinaseMaureen Wirschell
Department of Cell Biology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
Mol Biol Cell 15:2729-41. 2004..We propose that Oda5p is part of a novel axonemal complex that is required for outer arm assembly and anchors adenylate kinase in proximity to the arm...
Full-malaria 2004: an enlarged database for comparative studies of full-length cDNAs of malaria parasites, Plasmodium speciesJunichi Watanabe
Department of Parasitology, Human Genome Center, Institute of Medical Science, The University of Tokyo, 4 6 1, Shirokanedai, Minatoku, Tokyo 108 8639, Japan
Nucleic Acids Res 32:D334-8. 2004....
Expression of a major surface protein of Trypanosoma brucei insect forms is controlled by the activity of mitochondrial enzymesErik Vassella
Institute of Cell Biology, University of Bern, CH 3012 Bern, Switzerland
Mol Biol Cell 15:3986-93. 2004..Our results reveal a new mechanism by which expression of a surface glycoprotein is controlled by the activity of mitochondrial enzymes...
High coding density on the largest Paramecium tetraurelia somatic chromosomeMarek Zagulski
Institute of Biochemistry and Biophysics, DNA Sequencing Laboratory, Polish Academy of Sciences, Pawinskiego 5A, 02-106 Warsaw, Poland
Curr Biol 14:1397-404. 2004..Extrapolation to the whole genome suggests that Paramecium has at least 30,000 genes...
Targeting malaria virulence and remodeling proteins to the host erythrocyteMatthias Marti
Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria 3050, Australia
Science 306:1930-3. 2004..The conservation of this signal across Plasmodium species has implications for the development of new antimalarials...
TbRAB1 and TbRAB2 mediate trafficking through the early secretory pathway of Trypanosoma bruceiVivek Dhir
Department of Biological Sciences, Imperial College, London, SW7 2AY, UK
Mol Biochem Parasitol 137:253-65. 2004..These data indicate conservation of function for these proteins between trypanosomes and crown eukaryotes...
Plasmodium interspersed repeats: the major multigene superfamily of malaria parasitesChristoph S Janssen
Institute of Biomedical and Life Sciences, Division of Infection and Immunity, IBLS, University of Glasgow, Glasgow G12 8QQ, UK
Nucleic Acids Res 32:5712-20. 2004....
Transgene-mediated post-transcriptional gene silencing is inhibited by 3' non-coding sequences in ParameciumA Galvani
, CNRS, 91198 Gif-sur-Yvette Cedex, France
Nucleic Acids Res 29:4387-94. 2001....
Countrywide survey shows very high prevalence of Plasmodium falciparum multilocus resistance genotypes in CambodiaNimol Khim
Faculte de Pharmacie, UFR Sciences Pharmaceutiques, UMR INRA Université d Immunologie Parasitaire et de Vaccinologie, 31, avenue Monge Parc Grandmont, 37200 Tours, France
Antimicrob Agents Chemother 49:3147-52. 2005..Our findings show an alarming rate of multilocus resistance genotypes in Cambodia, requiring diligent surveillance and imposing limitations on possible future drug combinations...
Sub-grouping and sub-functionalization of the RIFIN multi-copy protein familyNicolas Joannin
Department of Microbiology, Tumor and Cell Biology MTC, Karolinska Institutet, SE 17177 Stockholm, Sweden and Swedish Institute for Infectious Diseases Control, SE 17182 Stockholm, Sweden
BMC Genomics 9:19. 2008....
Regulation of sexual development of Plasmodium by translational repressionGunnar R Mair
Department of Parasitology, Leiden University Medical Centre, 2333 ZA Leiden, Netherlands
Science 313:667-9. 2006..Genetic disruption of pbdozi inhibits the formation of the ribonucleoprotein complexes, and instead, at least 370 transcripts are diverted to a degradation pathway...
Molecular and phylogenetic characterization of syntaxin genes from parasitic protozoaJoel B Dacks
Program in Evolutionary Biology, Department of Biochemistry and Molecular Biology, Dalhousie University, 5850 College Street, Halifax, NS, Canada B3H 1X5
Mol Biochem Parasitol 136:123-36. 2004..Because of their integral role in membrane trafficking, the syntaxin genes represent a valuable potential molecular marker for the experimental study of the endomembrane system of disease-causing protists...
Two linked genes of Leishmania infantum encode tryparedoxins localised to cytosol and mitochondrionHelena Castro
Institute for Molecular and Cell Biology, Rua do Campo Alegre 823, 4150-180 Porto, Portugal
Mol Biochem Parasitol 136:137-47. 2004..infantum...
Characterization of the Trypanosoma cruzi Rad51 gene and its role in recombination events associated with the parasite resistance to ionizing radiationCarlos Gustavo Regis-da-Silva
Department of Biochemistry and Immunology, ICB, Universidade Federal de Minas Gerais, , 6627, Caixa Postal 486, Belo Horizonte 30161-970, MG, Brazil
Mol Biochem Parasitol 149:191-200. 2006..Furthermore, transfected parasites over-expressing TcRad51 have a faster kinetics of recovery of the normal pattern of chromosomal bands after irradiation as well as a higher resistance to zeocin treatment than do wild-type cultures...
The beta-N-acetylhexosaminidase of Entamoeba histolytica is composed of two homologous chains and has been localized to cytoplasmic granulesSabine Riekenberg
Department of Biochemistry, University of Osnabrueck, 49069 Osnabrueck, Germany
Mol Biochem Parasitol 138:217-25. 2004..Its localization in cytoplasmic granules strengthens the notion that the hexosaminidase complex may contribute to amoebic pathogenicity...
Mating-induced shedding of cell walls, removal of walls from vegetative cells, and osmotic stress induce presumed cell wall genes in ChlamydomonasXenia-Katharina Hoffmann
Institute of Biology III, Albert-Ludwigs-University, Freiburg, Germany
Plant Physiol 139:999-1014. 2005..Also under such conditions an activation of the genes was observed, suggesting that the signaling pathway(s) is (are) activated by wall removal itself...
Identification of a gene induced in conjugation-promoted cells of toxic marine dinoflagellates Alexandrium tamarense and Alexandrium catenella using differential display analysisShoko Hosoi-Tanabe
Department of Ecosystem Studies, School of Environmental Science, The University of Shiga Prefecture, Hikone, Shiga 522 8533, Japan
FEMS Microbiol Lett 251:161-8. 2005..Characterization of the identified gene might help in understanding the mechanism of encystment...
Specific DNA-binding by apicomplexan AP2 transcription factorsErandi K De Silva
Department of Molecular Biology and Lewis Sigler Institute for Integrative Genomics, Princeton University, 246 Carl Icahn Laboratory, Princeton, NJ 08544, USA
Proc Natl Acad Sci U S A 105:8393-8. 2008..Because of their origin in the plant lineage, ApiAP2 proteins have no homologues in the human host and may prove to be ideal antimalarial targets...
The role of metacaspase 1 in Plasmodium berghei development and apoptosisLudovic Le Chat
Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, United Kingdom
Mol Biochem Parasitol 153:41-7. 2007..We failed to detect an apparent loss-of-function phenotype, suggesting that PbMC1 constitutes a functionally redundant gene. We discuss these findings in the context of two other putative Plasmodium metacaspases that we describe here...
Direct observation of histone H2B-YFP fusion proteins and transport of their mRNA between conjugating ParameciaYasuhiro Takenaka
Department of Biochemistry, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305 8602, Japan
Gene 395:108-15. 2007..We concluded that at least the mRNA of histone H2B-PcVenus was transferred from the transformed, to the untransformed cell during conjugation...
Regulation of trypanosome DNA glycosylation by a SWI2/SNF2-like proteinCourtney DiPaolo
Global Infectious Diseases Program, Marine Biological Laboratory, Woods Hole, MA 02543, USA
Mol Cell 17:441-51. 2005..We propose a model in which chromatin remodeling by JBP2 regulates the initial sites of J synthesis within bloodstream form trypanosome DNA, with further propagation and maintenance of J by JBP1...
Contrasting signatures of selection on the Plasmodium falciparum erythrocyte binding antigen gene familyFederica Verra
Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, United Kingdom
Mol Biochem Parasitol 149:182-90. 2006..Population expansion simulations were performed under a neutral model, further supporting the departures from neutrality of these genes...
Identification and characterization of a novel alpha-kinase with a von Willebrand factor A-like motif localized to the contractile vacuole and Golgi complex in Dictyostelium discoideumVenkaiah Betapudi
Department of Physiology and Biophysics, Case Western Reserve University, Cleveland, OH 44106 4970, USA
Mol Biol Cell 16:2248-62. 2005..We propose that VwkA likely acts indirectly to influence myosin II abundance and assembly behavior and possibly has broader roles than previously characterized alpha kinases in this organism, which all seem to be MHCKs...
Isolation and characterization of Plasmodium falciparum UAP56 homolog: evidence for the coupling of RNA binding and splicing activity by site-directed mutationsJay Shankar
Malaria Group, International Centre for Genetic Engineering and Biotechnology, PO Box 10504, Aruna Asaf Ali Marg, New Delhi 110067, India
Arch Biochem Biophys 478:143-53. 2008..In the present study we have reported the detailed characterization of PfU52 from P. falciparum and these results advance the knowledge regarding the function of UAP56 in general...
Stage specific expression of poly(malic acid)-affiliated genes in the life cycle of Physarum polycephalum. Spherulin 3b and polymalataseNadthanan Pinchai
, Germany
FEBS J 273:1046-55. 2006..Quantitative PCR then revealed low levels of mRNA in amoebae, high levels in plasmodia, and also low levels in spherules, in agreement with the expression under transcriptional regulation in these cells...
Cloning and characterization of a Dictyostelium discoideum cDNA encoding a protein related to the medium chain subunit of clathrin-associated adaptor complexesL A Temesvari
Department of Microbiology and Immunology, Louisiana State University Medical Center, Shreveport 71130, USA
Gene 183:47-51. 1996..DdApm1 is the first reported full-length cDNA encoding a subunit of an AP complex in D. discoideum, and thus provides the first evidence for the existence of AP complexes in this organism...
Ser/Thr-rich repetitive motifs as targets for phosphoglycan modifications in Leishmania mexicana secreted acid phosphataseM Wiese
Max Planck Institut fur Biologie, Abteilung Membranbiochemie, Tubingen, Germany
EMBO J 14:1067-74. 1995..The Ser/Thr-rich domains are the targets for phosphoglycan modifications as shown by the expression of secreted fusion proteins composed of these C-terminal regions and the N-terminal domain of a lysosomal acid phosphatase...
LbrA, a protein predicted to have a role in vesicle trafficking, is necessary for normal morphogenesis in Polysphondylium pallidumY Kawabe
Institute of Biological Sciences, University of Tsukuba, Ibaraki, Japan
Gene 239:75-9. 1999..As far as we know, this is the first paper to show that a p24 family member is implicated in morphogenesis...
Sickle cell trait carriage: imbalanced distribution of IgG subclass antibodies reactive to Plasmodium falciparum family-specific MSP2 peptides in serum samples from Gabonese childrenFrancine Ntoumi
Centre International de Recherches Medicales de Franceville CIRMF, Franceville, Gabon
Immunol Lett 84:9-16. 2002..The role of the high multiclonal carriage associated with high family-specific antibodies reactive to MSP2 in HbAS children with asymptomatic P. falciparum parasitism is discussed...
Molecular cloning and cellular localization of a BiP homologue in Trypanosoma brucei. Divergent ER retention signals in a lower eukaryoteJ D Bangs
Department of Microbiology and Immunology, Stanford School of Medicine, CA 94305 5402
J Cell Sci 105:1101-13. 1993..1989) Biochemistry 28, 6440-6446), indicates that endoplasmic reticulum retrieval signals in trypanosomes may be as divergent and heterogeneous as any seen in the other eukaryotes yet studied...
Identification and disruption of the gene encoding the third member of the low-molecular-mass rhoptry complex in Plasmodium falciparumDeborah L Baldi
The Walter and Eliza Hall Institute of Medical Research, Melbourne 3050, Australia
Infect Immun 70:5236-45. 2002..falciparum; however, there is a single gene. Phylogenetic comparison of the RAP2/3 protein family from Plasmodium spp. suggests that the RAP2/3 duplication occurred after divergence of these parasite species...
Simultaneous expression of different variant-specific surface proteins in single Giardia lamblia trophozoites during encystationPedro G Carranza
, , , , Argentina
Infect Immun 70:5265-8. 2002..These results support the hypothesis that encystation and antigenic variation are processes that are mechanistically related...
Variation in the expression of a Plasmodium falciparum protein family implicated in erythrocyte invasionHelen M Taylor
National Institute for Medical Research, Mill Hill, London NW7 1AA, United Kingdom
Infect Immun 70:5779-89. 2002..The apparent redundancy in the PfRH family of proteins at the level of gene number and sequence and the variations in transcription and protein expression may allow the parasite to use alternative invasion pathways...
Analysis of a piwi-related gene implicates small RNAs in genome rearrangement in tetrahymenaKazufumi Mochizuki
Department of Biology, University of Rochester, Rochester, NY 14627, USA
Cell 110:689-99. 2002..We propose that these small RNAs function to specify sequences to be eliminated by a mechanism similar to RNA-mediated gene silencing...
Genes for glycosylphosphatidylinositol toxin biosynthesis in Plasmodium falciparumMauro Delorenzi
The Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria 3050, Australia
Infect Immun 70:4510-22. 2002..The biosynthetic pathway of the malaria parasite GPI offers potential targets for drug development and may be useful for studying parasite cell biology and the molecular basis for the pathophysiology of parasitic diseases...
Analysis of sequence diversity at the highly polymorphic Cpgp40/15 locus among Cryptosporidium isolates from human immunodeficiency virus-infected children in South AfricaBrett A Leav
Division of Geographic Medicine and Infectious Diseases, Tufts New England Medical Center, Boston, Massachusetts 02111, USA
Infect Immun 70:3881-90. 2002..Lastly, children infected with isolates having genotype Ic alleles were significantly older than those infected with isolates displaying other genotype I alleles...
Identification of a disulfide isomerase protein of Leishmania major as a putative virulence factorY Ben Achour
Laboratoire d'Immunologie (LAF301, Institut Pasteur de Tunis, , Tunisia
Infect Immun 70:3576-85. 2002..These findings suggest that LmPDI may play an important role in Leishmania natural pathogenicity and may constitute a new target for anti-Leishmania chemotherapy...
The Babesia bovis merozoite surface antigen 2 locus contains four tandemly arranged and expressed genes encoding immunologically distinct proteinsMonica Florin-Christensen
Program in Vector-Borne Diseases, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164-7040, USA
Infect Immun 70:3566-75. 2002..The results support the evolution of the msa-2 locus through at least two gene duplications, with selection for multiple related but antigenically distinct merozoite surface proteins...
Targeted disruption of expression site-associated gene-1 in bloodstream-form Trypanosoma bruceiV B Carruthers
Laboratory of Molecular Parasitology, Rockefeller University, New York, NY 10021 6399, USA
Mol Biochem Parasitol 81:65-79. 1996..We have therefore been unable to determine whether esag1 is an essential gene, or what function it fulfils, or whether any competent Esag1 protein is expressed in the mutant trypanosomes...
Structure and expression of a post-transcriptionally regulated malaria gene encoding a surface protein from the sexual stages of Plasmodium bergheiM G Paton
Department of Biology, Imperial College of Science, Technology and Medicine, London, UK
Mol Biochem Parasitol 59:263-75. 1993..The Pbs21 gene and the P. berghei parasite system provide an excellent vehicle for the study of stage-specific transcriptional and post-transcriptional control in malaria...
A second functional delta5 fatty acid desaturase in the cellular slime mould Dictyostelium discoideumT Saito
Division of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo, Japan
Eur J Biochem 267:1813-8. 2000..The substrate specificities of the two functional Delta5 desaturases of D. discoideum were also examined...
A novel Dictyostelium gene encoding multiple repeats of adhesion inhibitor-like domains has effects on cell-cell and cell-substrate adhesionTimothy R Varney
Department of Biological Sciences, University of Maryland Baltimore County, 1000 Hilltop Circle, Baltimore, MD 21250, USA
Dev Biol 243:226-48. 2002..We propose that the AmpA protein functions as an anti-adhesive to limit cell-cell and cell-substrate adhesion during development and thus facilitates cell migration during morphogenesis...
The gene family of EF-hand calcium-binding proteins from the flagellum of Trypanosoma bruceiY Wu
Department of Biological Sciences, Southern Methodist University, Dallas, TX 75235
Biochem J 304:833-41. 1994..We demonstrate that this variability resulted from amino acid substitutions at the N-terminus, C-terminal extensions, and duplication of internal segments...
Cloning and characterization of the G-box binding factor, an essential component of the developmental switch between early and late development in DictyosteliumG R Schnitzler
Department of Biology, University of California, San Diego, La Jolla 92093 0634
Genes Dev 8:502-14. 1994..Our results indicate that GBF acts as an extracellular cAMP-responsive transcriptional activator regulating late gene expression and is an essential component of a developmental switch between aggregation and cellular morphogenesis...
Cloning and characterization of a novel Plasmodium falciparum sporozoite surface antigen, STARPD A Fidock
Laboratory of Biomedical Parasitology, Pasteur Institute, Paris, France
Mol Biochem Parasitol 64:219-32. 1994..Identification and elucidation of this novel antigen is a step forward in current efforts aimed at developing an effective preerythrocytic-stage malaria vaccine...
Association of severe noncerebral Plasmodium falciparum malaria in Brazil with expressed PfEMP1 DBL1 alpha sequences lacking cysteine residuesKarin Kirchgatter
Departmento de Parasitologia, , , Brazil
Mol Med 8:16-23. 2002..CONCLUSIONS: This is the first report showing an association of severe noncerebral malaria from Brazil with particular DBL1 alpha sequences...
Molecular cloning, characterization and localization of PfPK4, an eIF-2alpha kinase-related enzyme from the malarial parasite Plasmodium falciparumJ J Möhrle
Department of Structural Biology, Biozentrum, University of Basel, Klingelbergstrasse 70, CH 4056 Basel, Switzerland
Biochem J 328:677-87. 1997..PfPK4 co-localizes with P. falciparum 83 kDa antigen/apical membrane antigen-1 at the apical complex in segmenters and merozoites, but does not co-localize with rhoptry-associated protein-1...
Excess polymorphisms in genes for membrane proteins in Plasmodium falciparumSarah K Volkman
Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, MA 02115, USA
Science 298:216-8. 2002..Genetic variation is also enriched in subtelomeric regions, which account for 22% of the chromosome but 76% of the polymorphisms...
Structure, organization and expression of the genes encoding mitochondrial cytochrome c(1) and the Rieske iron-sulfur protein in Chlamydomonas reinhardtiiA Atteia
Departamento de Genetica Molecular, Instituto de Fisiologia Celular, Universidad Nacional Autonoma de Mexico, Apartado Postal 70 243, 04510 Mexico D F, Mexico
Mol Genet Genomics 268:637-44. 2003..These data show that, in the green alga C. reinhardtii, the subunits of the cytochrome bc(1) complex fail to assemble properly in the absence of cytochrome b...
Incorporation of iron into Tritrichomonas foetus cell compartments reveals ferredoxin as a major iron-binding protein in hydrogenosomesPavel Suchan
Department of Parasitology, Faculty of Science, Charles University, , 128 44, Prague 2, Czech Republic
Microbiology 149:1911-21. 2003..Analysis of Tt. foetus hydrogenosomes showed at least nine iron-binding compounds, which were absent in metronidazole-resistant mutants. The major iron-binding compound was identified as [2Fe-2S] ferredoxin of the adrenodoxin type...
Research Grants
- Malaria HATs in Transcription RegulationLiwang Cui; Fiscal Year: 2010..This study aims to characterize the functions of a group of enzymes that regulate parasite gene expression, which may lead to the design of novel antimalarial drugs. ..
- HYPOTRICH GENOME IN DEVELOPMENT AND EVOLUTIONDavid Prescott; Fiscal Year: 2001..The experiments deal with the general problem of how an organism can manipulate its own DNA. ..
- MOLECULAR GENETICS OF LEISHMANIAStephen Beverley; Fiscal Year: 1999..This information will lead to improved protocols for recovering mutants, which is essential for functional genetic screens. ..
- Gene Duplication and Genome EvolutionAustin Hughes; Fiscal Year: 2004..5) Examining the role of transposable elements in duplication of genomic segments by testing for nonrandom association between these elements and putatively duplicated blocks in the yeast genome. ..
- MOLECULAR GENETICS OF LEISHMANIAStephen Beverley; Fiscal Year: 2005..Promising preliminary data with DNA microarrays have been gathered, and a series of investigations of Leishmania gene expression under a variety of relevant developmental stages and environmental treatments will be carried out. ..
- GLYCOSYLATION MUTANTS OF LEISHMANIAStephen Beverley; Fiscal Year: 2006..As one example, we will study a genetic model (phosphoglycan-deficiency) which separates acute virulence from persistence, and explore its utility in vaccine strategies. ..
- EVOLUTION OF IMMUNE RECOGNITION AND EVASIONAustin Hughes; Fiscal Year: 2002....
- GLYCOSYLATION MUTANTS OF LEISHMANIAStephen Beverley; Fiscal Year: 2003..As one example, we will study a genetic model (phosphoglycan-deficiency) which separates acute virulence from persistence, and explore its utility in vaccine strategies. ..
- EVOLUTION OF IMMUNE RECOGNITION AND EVASIONAustin Hughes; Fiscal Year: 2006..abstract_text> ..
- GLYCOSYLATION MUTANTS OF LEISHMANIAStephen Beverley; Fiscal Year: 2007..As one example, we will study a genetic model (phosphoglycan-deficiency) which separates acute virulence from persistence, and explore its utility in vaccine strategies. ..
- The Plasmodium 2TM and PHIST protein familiesThomas J Templeton; Fiscal Year: 2010..The expression, cellular localization, and phenotype following targeted gene disruption will be studied for select PHIST domain proteins toward an understanding of their function within the erythrocyte cytoplasm. ..
- MOLECULAR GENETICS OF LEISHMANIAStephen Beverley; Fiscal Year: 2009..abstract_text> ..
- EVOLUTION OF IMMUNE RECOGNITION AND EVASIONAustin Hughes; Fiscal Year: 2009..In addition, by increasing our understanding of the population diversity and evolutionary history of human immune system genes, this research will enhance our knowledge of human immune function in health and disease. ..
- GLYCOSYLATION MUTANTS OF LEISHMANIAStephen Beverley; Fiscal Year: 2009..Our ultimate goal is a comprehensive understanding of the genes and gene products responsible for synthesizing Leishmania surface and secreted molecules, and their individual and specific roles in parasite virulence. ..
- MOLECULAR GENETICS OF LEISHMANIAStephen M Beverley; Fiscal Year: 2010..abstract_text> ..
- EVOLUTION OF IMMUNE RECOGNITION AND EVASIONAustin L Hughes; Fiscal Year: 2010..In addition, by increasing our understanding of the population diversity and evolutionary history of human immune system genes, this research will enhance our knowledge of human immune function in health and disease. ..
- DRUG RESISTANCE AND PTERIDINE METABOLISM IN LEISHMANIAStephen M Beverley; Fiscal Year: 2010..These studies have yielded new opportunities for chemotherapeutic attack and new directions for future study of pteridine metabolism and biology. ..
- DRUG RESISTANCE AND GENE AMPLIFICATION IN LEISHMANIAStephen Beverley; Fiscal Year: 2007..Powerful genetic and bioinformatic screens will be used to identify new biopterin and folate utilizing enzymes and drug targets in aims 2 and 3. ..
- MOLECULAR GENETICS OF LEISHMANIAStephen Beverley; Fiscal Year: 2007....
- A new family of secreted adhesive proteins in PlasmodiumTHOMAS TEMPLETON; Fiscal Year: 2007....
- Malaria HATs in Transcription RegulationLiwang Cui; Fiscal Year: 2007..This study aims to characterize the functions of a group of enzymes that regulate parasite gene expression, which may lead to the design of novel antimalarial drugs. ..
- EVOLUTION OF IMMUNE RECOGNITION AND EVASIONAustin Hughes; Fiscal Year: 2007..In addition, by increasing our understanding of the population diversity and evolutionary history of human immune system genes, this research will enhance our knowledge of human immune function in health and disease. ..
- Plasmodium vivax:Proteomic Analysis of Asexual and Sporozoite StagesLiwang Cui; Fiscal Year: 2007..Its recent resurgence in prevalence and emergence of drug- resistant parasite strains have raised a great concern for the future control of malaria. ..
- GLYCOSYLATION MUTANTS OF LEISHMANIASalvatore J Turco; Fiscal Year: 2010..Our ultimate goal is a comprehensive understanding of the genes and gene products responsible for synthesizing Leishmania surface and secreted molecules, and their individual and specific roles in parasite virulence. ..
- REPEATED GENES: ORGANIZATION, EVOLUTION AND FUNCTIONMARTIN GOROVSKY; Fiscal Year: 1980..The sequence of Tetrahymena histones and of their sites of secondary modification will be examined to determine the relationships between functional and evolutionary divergence. ..
- Kinetoplastid SL RNA biogenesisDavid Campbell; Fiscal Year: 2007..Specific inhibition of the trans-splicing pathway is lethal to the cell and thus a prime target for anti-parasite intervention. ..
- RNAi in DNA Sequence Elimination in TetrahymenaMARTIN GOROVSKY; Fiscal Year: 2007..These studies should shed light on other RNAi- like processes that are important for normal development and maintenance of genome stability in multicellular organisms. ..
- Comparative Evolutionary Genomics and Infectious DiseaseJane Carlton; Fiscal Year: 2010....
- RNAi in DNA Sequence Elimination in TetrahymenaMartin A Gorovsky; Fiscal Year: 2010..These studies should shed light on other RNAi- like processes that are important for normal development and maintenance of genome stability in multicellular organisms. ..
- EXPRESSION OF THE LEISHMANIA TARENTOLAE SL RNA GENEDavid Campbell; Fiscal Year: 2003..These unique features represent potential targets for selective therapeutic intervention. ..
- REPEATED GENES--ORGANIZATION, EVOLUTION AND FUNCTIONMARTIN GOROVSKY; Fiscal Year: 2000..no abstract available ..
- REPEATED GENES-- ORGANIZATION, EVOLUTION, AND FUNCTIONMARTIN GOROVSKY; Fiscal Year: 2004....
