protozoan genes

Summary

Summary: The functional hereditary units of PROTOZOA.

Top Publications

  1. ncbi The genome of the African trypanosome Trypanosoma brucei
    Matthew Berriman
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton CB10 1SA, UK
    Science 309:416-22. 2005
  2. ncbi Genome of the host-cell transforming parasite Theileria annulata compared with T. parva
    Arnab Pain
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    Science 309:131-3. 2005
  3. pmc The transcriptome of the intraerythrocytic developmental cycle of Plasmodium falciparum
    Zbynek Bozdech
    Department of Biochemistry and Biophysics, University of California, San Francisco, USA
    PLoS Biol 1:E5. 2003
  4. pmc Chloroquine resistance in Plasmodium falciparum malaria parasites conferred by pfcrt mutations
    Amar bir Singh Sidhu
    Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Science 298:210-3. 2002
  5. pmc Plasmodium falciparum variant surface antigen expression patterns during malaria
    Peter C Bull
    Nuffield Department of Clinical Medicine, John Radcliffe Hospital, University of Oxford, Oxford, United Kingdom
    PLoS Pathog 1:e26. 2005
  6. ncbi The genome sequence of Trypanosoma cruzi, etiologic agent of Chagas disease
    Najib M El-Sayed
    Department of Parasite Genomics, Institute for Genomic Research, Rockville, MD 20850, USA
    Science 309:409-15. 2005
  7. pmc Draft genome sequence of the sexually transmitted pathogen Trichomonas vaginalis
    Jane M Carlton
    Institute for Genomic Research, 9712 Medical Research Drive, Rockville, MD 20850, USA
    Science 315:207-12. 2007
  8. ncbi Widespread functional specialization of Plasmodium falciparum erythrocyte membrane protein 1 family members to bind CD36 analysed across a parasite genome
    Bridget A Robinson
    Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80525, USA
    Mol Microbiol 47:1265-78. 2003
  9. ncbi Genomic minimalism in the early diverging intestinal parasite Giardia lamblia
    Hilary G Morrison
    Marine Biological Laboratory, Woods Hole, MA 02543 1015, USA
    Science 317:1921-6. 2007
  10. ncbi P. falciparum rosetting mediated by a parasite-variant erythrocyte membrane protein and complement-receptor 1
    J A Rowe
    Laboratory of Parasitic Diseases, NIAID, NIH, Bethesda, Maryland 20892, USA
    Nature 388:292-5. 1997

Detail Information

Publications253 found, 100 shown here

  1. ncbi The genome of the African trypanosome Trypanosoma brucei
    Matthew Berriman
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton CB10 1SA, UK
    Science 309:416-22. 2005
    ..brucei and the greatest in L. major. Horizontal transfer of genes of bacterial origin has contributed to some of the metabolic differences in these parasites, and a number of novel potential drug targets have been identified...
  2. ncbi Genome of the host-cell transforming parasite Theileria annulata compared with T. parva
    Arnab Pain
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    Science 309:131-3. 2005
    ....
  3. pmc The transcriptome of the intraerythrocytic developmental cycle of Plasmodium falciparum
    Zbynek Bozdech
    Department of Biochemistry and Biophysics, University of California, San Francisco, USA
    PLoS Biol 1:E5. 2003
    ..falciparum and provide a resource for the identification of new chemotherapeutic and vaccine candidates...
  4. pmc Chloroquine resistance in Plasmodium falciparum malaria parasites conferred by pfcrt mutations
    Amar bir Singh Sidhu
    Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Science 298:210-3. 2002
    ..pfcrt mutations increased susceptibility to artemisinin and quinine and minimally affected amodiaquine activity; hence, these antimalarials warrant further investigation as agents to control chloroquine-resistant falciparum malaria...
  5. pmc Plasmodium falciparum variant surface antigen expression patterns during malaria
    Peter C Bull
    Nuffield Department of Clinical Medicine, John Radcliffe Hospital, University of Oxford, Oxford, United Kingdom
    PLoS Pathog 1:e26. 2005
    ....
  6. ncbi The genome sequence of Trypanosoma cruzi, etiologic agent of Chagas disease
    Najib M El-Sayed
    Department of Parasite Genomics, Institute for Genomic Research, Rockville, MD 20850, USA
    Science 309:409-15. 2005
    ....
  7. pmc Draft genome sequence of the sexually transmitted pathogen Trichomonas vaginalis
    Jane M Carlton
    Institute for Genomic Research, 9712 Medical Research Drive, Rockville, MD 20850, USA
    Science 315:207-12. 2007
    ..The genome sequence predicts previously unknown functions for the hydrogenosome, which support a common evolutionary origin of this unusual organelle with mitochondria...
  8. ncbi Widespread functional specialization of Plasmodium falciparum erythrocyte membrane protein 1 family members to bind CD36 analysed across a parasite genome
    Bridget A Robinson
    Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80525, USA
    Mol Microbiol 47:1265-78. 2003
    ..These can be used to investigate the role of proteins in malaria pathogenesis and potentially tailor vaccines to recognize particular binding variants...
  9. ncbi Genomic minimalism in the early diverging intestinal parasite Giardia lamblia
    Hilary G Morrison
    Marine Biological Laboratory, Woods Hole, MA 02543 1015, USA
    Science 317:1921-6. 2007
    ..This genome sequence will not only be valuable for investigating the evolution of eukaryotes, but will also be applied to the search for new therapeutics for this parasite...
  10. ncbi P. falciparum rosetting mediated by a parasite-variant erythrocyte membrane protein and complement-receptor 1
    J A Rowe
    Laboratory of Parasitic Diseases, NIAID, NIH, Bethesda, Maryland 20892, USA
    Nature 388:292-5. 1997
    ..Thus we describe a new adhesive function for PfEMP1 and raise the possibility that CR1 polymorphisms in Africans that influence the interaction between erythrocytes and PfEMP1 may protect against severe malaria...
  11. pmc Genome sequence of Babesia bovis and comparative analysis of apicomplexan hemoprotozoa
    Kelly A Brayton
    Program in Genomics, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, USA
    PLoS Pathog 3:1401-13. 2007
    ..The genome sequence provides a greater understanding of B. bovis metabolism and potential avenues for drug therapies and vaccine development...
  12. ncbi A pol I transcriptional body associated with VSG mono-allelic expression in Trypanosoma brucei
    M Navarro
    Present address Instituto de Parasitología y Biomedicina López Neyra, CSIC, C Ventanilla 11, 18001 Granada, Spain
    Nature 414:759-63. 2001
    ..We propose that the recruitment of a single expression site and the concomitant exclusion of inactive loci from a discrete transcriptional body define the mechanism responsible for VSG mono-allelic expression...
  13. ncbi Phylogenetic analyses of diplomonad genes reveal frequent lateral gene transfers affecting eukaryotes
    Jan O Andersson
    The Canadian Institute for Advanced Research, Program in Evolutionary Biology, Department of Biochemistry, Dalhousie University, B3H 4H7, Halifax, Nova Scotia, Canada
    Curr Biol 13:94-104. 2003
    ..Here, we perform a systematic analysis to investigate the impact of LGT on the evolution of diplomonads, a group of anaerobic protists...
  14. ncbi Global trends of whole-genome duplications revealed by the ciliate Paramecium tetraurelia
    Jean Marc Aury
    Genoscope and CNRS UMR 8030, 2 rue Gaston Crémieux CP5706, 91057 Evry, France
    Nature 444:171-8. 2006
    ..The conclusion of this analysis is that many genes are maintained after whole-genome duplication not because of functional innovation but because of gene dosage constraints...
  15. ncbi Discovery of gene function by expression profiling of the malaria parasite life cycle
    Karine G Le Roch
    Department of Cell Biology ICND202, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Science 301:1503-8. 2003
    ..Genes with highly correlated levels and temporal patterns of expression were often involved in similar functions or cellular processes...
  16. pmc The intestinal protozoan parasite Entamoeba histolytica contains 20 cysteine protease genes, of which only a small subset is expressed during in vitro cultivation
    Iris Bruchhaus
    Bernhard Nocht Institute for Tropical Medicine, 20359 Hamburg, Germany
    Eukaryot Cell 2:501-9. 2003
    ..histolytica or E. dispar trophozoites during in vitro cultivation. Therefore, it is likely that at least some of these enzymes are required for infection of the human host and/or for completion of the parasite life cycle...
  17. ncbi A secreted serine-threonine kinase determines virulence in the eukaryotic pathogen Toxoplasma gondii
    S Taylor
    Department of Molecular Microbiology, Washington University School of Medicine, St Louis, MO 63130, USA
    Science 314:1776-80. 2006
    ..These attributes of ROP18 required kinase activity, which revealed that secretion of effectors is a major component of parasite virulence...
  18. ncbi Trypanosoma cruzi surface mucins: host-dependent coat diversity
    Carlos A Buscaglia
    Instituto de Investigaciones Biotecnologicas Instituto Tecnologico de Chascomus IIB INTECH, Universidad Nacional de General San Martn CONICET, San Martin 1650, Buenos Aires, Argentina
    Nat Rev Microbiol 4:229-36. 2006
    ..Here, we discuss the expression profiling of T. cruzi mucins, the mechanisms leading to the acquisition of mucin diversity and the possible consequences of a mosaic surface coat in the interplay between parasite and host...
  19. ncbi Analysis of a piwi-related gene implicates small RNAs in genome rearrangement in tetrahymena
    Kazufumi Mochizuki
    Department of Biology, University of Rochester, Rochester, NY 14627, USA
    Cell 110:689-99. 2002
    ..We propose that these small RNAs function to specify sequences to be eliminated by a mechanism similar to RNA-mediated gene silencing...
  20. ncbi Improvements in transfection efficiency and tests of RNA interference (RNAi) approaches in the protozoan parasite Leishmania
    Kelly A Robinson
    Department of Molecular Microbiology, Washington University Medical School, 660 S Euclid Avenue, St Louis, MO 63110, USA
    Mol Biochem Parasitol 128:217-28. 2003
    ..The implications to parasite biology, gene amplification, and genetic analysis are discussed...
  21. pmc Association of severe noncerebral Plasmodium falciparum malaria in Brazil with expressed PfEMP1 DBL1 alpha sequences lacking cysteine residues
    Karin Kirchgatter
    Departmento de Parasitologia, Instituto de Ciencias Biomedicas, Universidade de Sao Paulo, Brazil
    Mol Med 8:16-23. 2002
    ..We analyzed parasites of Brazilian patients with severe malaria to determine whether there were particular DBL1 alpha var sequences predominantly expressed in such patients...
  22. ncbi Telomeric heterochromatin propagation and histone acetylation control mutually exclusive expression of antigenic variation genes in malaria parasites
    Lucio H Freitas-Junior
    Institut Pasteur, Biology of Host Parasite Interactions Unit CNRS URA2581, 25, rue du Dr Roux, 75724 Paris, France
    Cell 121:25-36. 2005
    ..Our data demonstrate that mutually exclusive transcription of var genes is linked to the dynamic remodeling of chromatin...
  23. pmc Germ line transcripts are processed by a Dicer-like protein that is essential for developmentally programmed genome rearrangements of Tetrahymena thermophila
    Colin D Malone
    Biology Department, Washington University, St Louis, MO 63130, USA
    Mol Cell Biol 25:9151-64. 2005
    ....
  24. ncbi Targeting malaria virulence and remodeling proteins to the host erythrocyte
    Matthias Marti
    Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria 3050, Australia
    Science 306:1930-3. 2004
    ..The conservation of this signal across Plasmodium species has implications for the development of new antimalarials...
  25. ncbi A host-targeting signal in virulence proteins reveals a secretome in malarial infection
    N Luisa Hiller
    Departments of Pathology and Microbiology Immunology, Feinberg School of Medicine, Northwestern University, 303 East Chicago Avenue, Chicago, IL 60611, USA
    Science 306:1934-7. 2004
    ..Erythrocyte modification by the parasite may involve plasmodial heat shock proteins and be vastly more complex than hitherto realized...
  26. ncbi Reticulocyte-binding protein homologue 1 is required for sialic acid-dependent invasion into human erythrocytes by Plasmodium falciparum
    Tony Triglia
    The Walter and Eliza Hall Institute of Medical Research, Melbourne 3050, Australia
    Mol Microbiol 55:162-74. 2005
    ..falciparum parasites to utilize alternative receptors on the erythrocyte surface for evasion of receptor polymorphisms and the host immune system...
  27. ncbi A study of genetic diversity in the gene encoding the circumsporozoite protein (CSP) of Plasmodium falciparum from different transmission areas--XVI. Asembo Bay Cohort Project
    Ananias A Escalante
    Department of Biology, Emory University, Atlanta, GA, USA
    Mol Biochem Parasitol 125:83-90. 2002
    ..We conclude that it is inappropriate to use linkage-recombination patterns on genes under positive selection for assessing the structure of parasite populations...
  28. pmc Horizontal gene transfer from Bacteria to rumen Ciliates indicates adaptation to their anaerobic, carbohydrates-rich environment
    Guenola Ricard
    Center for Molecular and Biomolecular Informatics, Nijmegen Center for Molecular Life Sciences, Radboud University Nijmegen Medical Centre, Toernooiveld 1, 6525 ED Nijmegen, The Netherlands
    BMC Genomics 7:22. 2006
    ....
  29. pmc Comparative analysis of apicomplexa and genomic diversity in eukaryotes
    Thomas J Templeton
    Department of Microbiology and Immunology, Weill Medical College and the Program in Immunology and Microbial Pathogenesis, Weill Graduate School of Medical Sciences of Cornell University, New York, New York 10021, USA
    Genome Res 14:1686-95. 2004
    ..We also describe additional molecular characteristics distinguishing Apicomplexa from other eukaryotes for which complete genome sequences are available...
  30. pmc Polymorphic secreted kinases are key virulence factors in toxoplasmosis
    J P J Saeij
    Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA
    Science 314:1780-3. 2006
    ..Both are hypervariable rhoptry proteins that are secreted into the host cell upon invasion. These results suggest that secreted kinases unique to the Apicomplexa are crucial in the host-pathogen interaction...
  31. ncbi Comparative genomics of trypanosomatid parasitic protozoa
    Najib M El-Sayed
    Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA
    Science 309:404-9. 2005
    ..Contrary to recent reports, our analyses reveal no evidence that these species are descended from an ancestor that contained a photosynthetic endosymbiont...
  32. ncbi Expression and function of pvcrt-o, a Plasmodium vivax ortholog of pfcrt, in Plasmodium falciparum and Dictyostelium discoideum
    Juliana Martha Sá
    Departamento de Parasitologia, Instituto de Ciencias Biomedicas, Universidade de Sao Paulo, Av Lineu Prestes 1374, Sao Paulo 05508 900, SP, Brazil
    Mol Biochem Parasitol 150:219-28. 2006
    ..Our data support an effect of PvCRT-o on chloroquine transport and/or accumulation by P. vivax, independent of the K76T amino acid substitution...
  33. pmc Extragenic bypass suppressors of mutations in the essential gene BLD2 promote assembly of basal bodies with abnormal microtubules in Chlamydomonas reinhardtii
    A M Preble
    Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, Colorado 80309-0347, USA
    Genetics 157:163-81. 2001
    ..It is likely that BLD2 is an essential gene that is needed for basal body assembly and function...
  34. pmc Active transcription is required for maintenance of epigenetic memory in the malaria parasite Plasmodium falciparum
    Ron Dzikowski
    Department of Parasitology, The Kuvin Center for the Study of Infectious and Tropical Diseases, The Institute for Medical Research, The Hebrew University Hadassah Medical School, Jerusalem 91120, Israel
    J Mol Biol 382:288-97. 2008
    ....
  35. ncbi Protection against Leishmania donovani infection by DNA vaccination: increased DNA vaccination efficiency through inhibiting the cellular p53 response
    A Ghosh
    Department of Microbiology and Immunology, McGill University, 3775 University Street, Montreal, H3A 2B4, Quebec, Canada
    Vaccine 19:3169-78. 2001
    ..These results have important implications for DNA vaccination against leishmaniasis and potentially against other infectious diseases...
  36. ncbi A phylogenetic comparison of gene trees constructed from plastid, mitochondrial and genomic DNA of Plasmodium species
    D Rathore
    Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 4 Center Drive MSC 0425, Bethesda, MD 20892-0425, USA
    Mol Biochem Parasitol 114:89-94. 2001
    ..falciparum...
  37. pmc Biology of Giardia lamblia
    R D Adam
    Department of Medicine, University of Arizona College of Medicine, 1501N Campbell, Tucson, AZ 85724 5049, USA
    Clin Microbiol Rev 14:447-75. 2001
    ..The Giardia genome project promises to greatly increase our understanding of this interesting and enigmatic organism...
  38. pmc A cluster of ring stage-specific genes linked to a locus implicated in cytoadherence in Plasmodium falciparum codes for PEXEL-negative and PEXEL-positive proteins exported into the host cell
    Tobias Spielmann
    Infectious Diseases and Immunology Division, Queensland Institute of Medical Research, PO Royal Brisbane Hospital, Herston QLD 4029, Australia
    Mol Biol Cell 17:3613-24. 2006
    ..Biochemical and photobleaching studies using these proteins reveal new properties of the parasite-induced membrane compartments in the host cell. This has important implications for the biogenesis and connectivity of these structures...
  39. pmc Large-scale intron conservation and order-of-magnitude variation in intron loss/gain rates in apicomplexan evolution
    Scott William Roy
    Allan Wilson Centre for Molecular Ecology and Evolution, Massey University, Palmerston North, New Zealand
    Genome Res 16:1270-5. 2006
    ..We suggest that intron loss/gain in some eukaryotic lineages may be concentrated in relatively short episodes coincident with occasional TE invasions...
  40. ncbi Dinoflagellate expressed sequence tag data indicate massive transfer of chloroplast genes to the nuclear genome
    Tsvetan R Bachvaroff
    Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD 20742, USA
    Protist 155:65-78. 2004
    ..A preliminary report of these data was presented at the Botany 2002 meeting in Madison, WI...
  41. ncbi Antigenic variation in Plasmodium falciparum
    Artur Scherf
    Biology of Host Parasite Interactions Unit, CNRS URA2581, Institut Pasteur 75724 Paris, France
    Annu Rev Microbiol 62:445-70. 2008
    ..In addition, controversies surrounding var genetic elements in antigenic variation are discussed...
  42. pmc The genome of the simian and human malaria parasite Plasmodium knowlesi
    A Pain
    Wellcome Trust Sanger Institute, Genome Campus, Hinxton, Cambridgeshire CB10 1SA, UK
    Nature 455:799-803. 2008
    ..One of these families, the KIRs, contains sequences that collectively match over one-half of the host CD99 extracellular domain, which may represent an unusual form of molecular mimicry...
  43. ncbi Intercontinental spread of pyrimethamine-resistant malaria
    Cally Roper
    London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK
    Science 305:1124. 2004
    ..The resistance alleles carried by these migrants are now spreading across Africa at an alarming rate, signaling the end of affordable malaria treatment and presenting sub-Saharan Africa with a public health crisis...
  44. pmc Temporal order of RNA-processing reactions in trypanosomes: rapid trans splicing precedes polyadenylation of newly synthesized tubulin transcripts
    E Ullu
    Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut 06510 8056
    Mol Cell Biol 13:720-5. 1993
    ..Thus, trans splicing is cotranscriptional. In addition, we show that trans splicing precedes polyadenylation in the processing of trypanosome tubulin pre-mRNAs...
  45. ncbi Inhibitors of the nonmevalonate pathway of isoprenoid biosynthesis as antimalarial drugs
    H Jomaa
    Institute of Biochemistry, Academic Hospital Centre, Justus Liebig University, Friedrichstrasse 24, D 35392 Giessen, Germany
    Science 285:1573-6. 1999
    ..Both drugs suppressed the in vitro growth of multidrug-resistant P. falciparum strains. After therapy with these drugs, mice infected with the rodent malaria parasite P. vinckei were cured...
  46. ncbi Regulation of apicomplexan actin-based motility
    Jake Baum
    Division of Infection and Immunity, The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria 3050, Australia
    Nat Rev Microbiol 4:621-8. 2006
    ....
  47. ncbi Plasmodium vivax and the importance of the subtelomeric multigene vir superfamily
    Carmen Fernandez-Becerra
    Barcelona Centre for International Health Research, Hospital Clinic IDIBAPS, Universitat de Barcelona, Rosello 132, 08036 Barcelona, Spain
    Trends Parasitol 25:44-51. 2009
    ..vivax and from data gathered from experimental infections in reticulocyte-prone non-lethal malaria parasites and natural P. vivax infections...
  48. ncbi Multi-character population study of the vir subtelomeric multigene superfamily of Plasmodium vivax, a major human malaria parasite
    Emilio F Merino
    Departamento de Parasitologia, Instituto de Ciencias Biomedicas, Universidade de São, Paulo, Av Lineu Prestes 1374, Sao Paulo, SP 05508 900, Brazil
    Mol Biochem Parasitol 149:10-6. 2006
    ..These results suggest that VIR proteins can have different subcellular localizations and functions. This is the first study on a population level of the P. vivax vir subtelomeric multigene superfamily...
  49. ncbi Telomere and subtelomere of Trypanosoma cruzi chromosomes are enriched in (pseudo)genes of retrotransposon hot spot and trans-sialidase-like gene families: the origins of T. cruzi telomeres
    Dong Kim
    Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, UNIFESP, Rua Botucatu, 862, CEP 04023 062, S Paulo, Brazil
    Gene 346:153-61. 2005
    ..cruzi. Finally, a mechanism for generation of T. cruzi telomere by chromosome breakage and telomere healing is proposed...
  50. pmc Plasmodium falciparum associated with severe childhood malaria preferentially expresses PfEMP1 encoded by group A var genes
    Anja T R Jensen
    Department of Medical Microbiology and Immunology, The Panum Institute, Building 24 2, Blegdamsvej 3, 2200 Copenhagen, Denmark
    J Exp Med 199:1179-90. 2004
    ..The proteins encoded by Group A var genes, such as PFD1235w/MAL7P1.1, appear to be involved in the pathogenesis of severe disease and are thus attractive candidates for a vaccine against life-threatening P. falciparum malaria...
  51. ncbi Evidence for selection for the tyrosine-86 allele of the pfmdr 1 gene of Plasmodium falciparum by chloroquine and amodiaquine
    M T Duraisingh
    Department of Medical Parasitology, London School of Hygiene and Tropical Medicine, UK
    Parasitology 114:205-11. 1997
    ..The samples were genotyped at 3 unlinked polymorphic loci. These analyses suggested that most parasites observed at day 7 were probably recrudescences whereas most of those at day 28 were reinfections...
  52. pmc Nucleotide sequences provide evidence of genetic exchange among distantly related lineages of Trypanosoma cruzi
    C A Machado
    Department of Ecology and Evolutionary Biology, University of California, Irvine, CA 92697, USA
    Proc Natl Acad Sci U S A 98:7396-401. 2001
    ..We estimate that the major extant lineages of T. cruzi have diverged during the Miocene or early Pliocene (3-16 million years ago)...
  53. pmc The genome of the kinetoplastid parasite, Leishmania major
    Alasdair C Ivens
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire CB10 1SA, UK
    Science 309:436-42. 2005
    ..Abundant RNA-binding proteins are encoded in the Tritryp genomes, consistent with active posttranscriptional regulation of gene expression...
  54. ncbi Heterochromatin silencing and locus repositioning linked to regulation of virulence genes in Plasmodium falciparum
    Manoj T Duraisingh
    The Walter and Eliza Hall Institute of Medical Research, Melbourne 3050, Australia
    Cell 121:13-24. 2005
    ..Together, this implies that locus repositioning and heterochromatic silencing play important roles in the epigenetic regulation of virulence genes in P. falciparum...
  55. ncbi Detecting selection using a single genome sequence of M. tuberculosis and P. falciparum
    Joshua B Plotkin
    Harvard Society of Fellows and Bauer Center for Genomics Research, 7 Divinity Avenue, Cambridge, Massachusetts 02138, USA
    Nature 428:942-5. 2004
    ..Our method of estimating selective pressures requires far fewer data than comparative sequence analysis, and it measures selection across an entire genome; the method can readily be applied to a large range of sequenced organisms...
  56. pmc PiggyMac, a domesticated piggyBac transposase involved in programmed genome rearrangements in the ciliate Paramecium tetraurelia
    Céline Baudry
    CNRS FRE 3144, Centre de Genetique Moleculaire, Gif sur Yvette F 91198 Cedex, France
    Genes Dev 23:2478-83. 2009
    ..This is the first in vivo evidence suggesting an essential endonucleolytic function of a domesticated piggyBac transposase...
  57. ncbi The SRS superfamily of Toxoplasma surface proteins
    Calvin Jung
    Infectious Diseases, Department of Medicine, University of British Columbia, D459 HP East, 2733 Heather Street, Vancouver, BC, Canada V5Z 3J5
    Int J Parasitol 34:285-96. 2004
    ..Orthologous genes are restricted to tissue-dwelling coccidia (Neospora, Sarcocystis) with no related sequences present in other more distant apicomplexa such as Eimeria, Cryptosporidia, and Plasmodium spp...
  58. pmc Differential var gene transcription in Plasmodium falciparum isolates from patients with cerebral malaria compared to hyperparasitaemia
    Helen M Kyriacou
    Institute of Immunology and Infection Research, School of Biological Sciences, University of Edinburgh, Edinburgh EH9 3JT, UK
    Mol Biochem Parasitol 150:211-8. 2006
    ..These results suggest that var genes with DBLalpha1-like domains (Group A or B/A) may be implicated in the pathogenesis of cerebral malaria, while var genes with DBLalpha0-like domains promote less virulent malaria infections...
  59. pmc Gene amplification of the multidrug resistance 1 gene of Plasmodium vivax isolates from Thailand, Laos, and Myanmar
    Mallika Imwong
    Department of Clinical Tropical Medicine, Faculty of Tropical Medicine, Mahidol University, 420 6 Rajvithi Rd, Bangkok 10400, Thailand
    Antimicrob Agents Chemother 52:2657-9. 2008
    ..02). Five coding mutations in pvmdr1, independent of gene amplification, were also found...
  60. ncbi Identification of a sporozoite-specific member of the Toxoplasma SAG superfamily via genetic complementation
    Jay R Radke
    Department of Veterinary Molecular Biology, Montana State University, Bozeman, Montana 59717 3610, USA
    Mol Microbiol 52:93-105. 2004
    ..Expression of SporoSAG in tachyzoites conferred enhanced invasion on transgenic parasites suggesting a role for this protein in oocyst/sporozoite transmission to susceptible hosts...
  61. ncbi Global genetic diversity and evolution of var genes associated with placental and severe childhood malaria
    Adama R Trimnell
    Seattle Biomedical Research Institute, Seattle, WA, USA
    Mol Biochem Parasitol 148:169-80. 2006
    ..These studies aid our understanding of the evolutionary mechanisms that shape var diversity and will be important to the development of vaccines against pregnancy associated malaria and severe malaria...
  62. pmc Microarray analyses of gene expression during the Tetrahymena thermophila life cycle
    Wei Miao
    Department of Biology, University of Rochester, Rochester, New York, USA
    PLoS ONE 4:e4429. 2009
    ..The model eukaryote, Tetrahymena thermophila, is the first ciliated protozoan whose genome has been sequenced, enabling genome-wide analysis of gene expression...
  63. ncbi KIN241: a gene involved in cell morphogenesis in Paramecium tetraurelia reveals a novel protein family of cyclophilin-RNA interacting proteins (CRIPs) conserved from fission yeast to man
    A Krzywicka
    , Centre National de la Recherche Scientifique, 91198 Gif-sur-Yvette Cedex, France
    Mol Microbiol 42:257-67. 2001
    ..No Kin241-1 protein is present in mutant cells, suggesting that the C-terminal serine-rich region is responsible for protein stability...
  64. ncbi Antigenic variation in Babesia bovis occurs through segmental gene conversion of the ves multigene family, within a bidirectional locus of active transcription
    Basima Al-Khedery
    Department of Pathobiology, University of Florida, Gainesville, 32611, USA
    Mol Microbiol 59:402-14. 2006
    ..These results also suggest the possibility of epigenetic regulation through in situ switching among gene loci, further expanding the potential repertoire of variant proteins...
  65. pmc A spliceosomal intron in Giardia lamblia
    Julie E J Nixon
    Department of Immunology and Infectious Diseases, Harvard School of Public Health, 665 Huntington Avenue, Boston, MA 02115 6018, USA
    Proc Natl Acad Sci U S A 99:3701-5. 2002
    ..If introns were added during eukaryotic evolution (the introns-late hypothesis), then these results push back the date of this event before the branching of G. lamblia...
  66. ncbi Differential gene expression in Entamoeba histolytica isolated from amoebic liver abscess
    Iris Bruchhaus
    Bernhard Nocht Institute for Tropical Medicine, Bernhard Nocht Str 74, 20359 Hamburg, Germany
    Mol Microbiol 44:1063-72. 2002
    ....
  67. ncbi Genome sequence of Theileria parva, a bovine pathogen that transforms lymphocytes
    Malcolm J Gardner
    Institute for Genomic Research TIGR, 9712 Medical Center Drive, Rockville, MD 20850, USA
    Science 309:134-7. 2005
    ..Several biosynthetic pathways are incomplete or absent, suggesting substantial metabolic dependence on the host cell. One protein family that may generate parasite antigenic diversity is not telomere-associated...
  68. ncbi Genes induced by a high-oxygen environment in Entamoeba histolytica
    Md Ali Akbar
    Department of Microbiology, National Institute of Cholera and Enteric Diseases, P 33 C I T Road, Scheme XM, Kolkata 700 010, West Bengal, India
    Mol Biochem Parasitol 133:187-96. 2004
    ..signal transduction, tissue destruction, and oxidative stress management, including enhanced expression of a 29-kDa Eh29, suggest that this organism has several protective mechanisms to deal with oxidative stress during invasion...
  69. ncbi Direct visualisation of individual gene organisation in Trypanosoma brucei by high-resolution in situ hybridisation
    K Ersfeld
    School of Biological Sciences, 2 205 Stopford Building, University of Manchester, Manchester M13 9PT, UK
    Chromosoma 107:237-40. 1998
    ..We demonstrated the repetitive nature of the tubulin gene cluster, which consists of up to 19 alpha- and beta-tubulin genes arranged in tandem repeats...
  70. ncbi A sub-family of common and highly conserved Plasmodium falciparum var genes
    Ali Salanti
    Centre for Medical Parasitology at Institute for Medical Microbiology and Immunology, University of Copenhagen and Department of Infectious Diseases, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark
    Mol Biochem Parasitol 122:111-5. 2002
  71. ncbi Identification and molecular characterisation of DNA damaging agent induced expression of Plasmodium falciparum recombination protein PfRad51
    Mrinal Kanti Bhattacharyya
    Johns Hopkins University Bloomberg School of Public Health, 615 N Wolfe Street, Baltimore, MD 21205, USA
    Int J Parasitol 33:1385-92. 2003
    ..PfRad51 is the first apicomplexan gene identified that codes for a recombination protein, and it offers an excellent model for studying DNA damage inducible gene expression in such parasites...
  72. ncbi Rapid evolution of an erythrocyte invasion gene family: the Plasmodium reichenowi Reticulocyte Binding Like (RBL) genes
    Julian C Rayner
    Division of Parasitic Diseases, Centers for Disease Control and Prevention, National Center for Infectious Diseases, Atlanta, GA 30341, USA
    Mol Biochem Parasitol 133:287-96. 2004
    ..The RBL super-family, therefore, appears to be surprisingly dynamic and divergent, implying that it is involved in species-specific aspects of erythrocyte recognition and invasion...
  73. ncbi Organization, transcription, and expression of rhoptry associated protein genes in the Babesia bigemina rap-1 locus
    Carlos E Suarez
    Program in Vector Borne Diseases, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164 7040, USA
    Mol Biochem Parasitol 127:101-12. 2003
    ..Immunoblot analysis of merozoites revealed the expected RAP-1a expression but failed to detect expressed RAP-1b and RAP-1c, indicating that expression of the rap-1 genes is regulated both at the transcriptional and translational levels...
  74. pmc Dynamic RNA profiling in Plasmodium falciparum synchronized blood stages exposed to lethal doses of artesunate
    Onguma Natalang
    Institut Pasteur, Unité d Immunologie Moléculaire des Parasites, CNRS URA 2581, 28 rue du Docteur Roux, F 75724, Paris, Cedex 15, France
    BMC Genomics 9:388. 2008
    ..However, few studies to date have used this powerful approach to explore transcriptome alterations of P. falciparum parasites exposed to antimalarial drugs...
  75. ncbi Structure and content of the Entamoeba histolytica genome
    C G Clark
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London WC1E 7HT, UK
    Adv Parasitol 65:51-190. 2007
    ..In this chapter we summarise the features of the genome as they are currently understood and provide previously unpublished analyses of many of the genes...
  76. ncbi Presence of multiple mRNA cycling sequence element-binding proteins in Crithidia fasciculata
    Bidyottam Mittra
    Molecular Biology Institute and Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, California 90095, USA
    J Biol Chem 278:26564-71. 2003
    ..Three polypeptides associated with purified CSBP II show specific binding to the cycling sequence. These proteins may represent a family of sequence-specific RNA-binding proteins involved in post-transcriptional regulation...
  77. pmc A mitogen-activated protein (MAP) kinase homologue of Leishmania mexicana is essential for parasite survival in the infected host
    M Wiese
    Max Planck Institut fur Biologie, Abteilung Membranbiochemie, Corrensstrasse 38, D 72076 Tubingen, Germany
    EMBO J 17:2619-28. 1998
    ..Therefore, LMPK is a kinase that is essential for the survival of L.mexicana in the infected host by affecting the cell division of the amastigotes...
  78. pmc Adaptations of Trypanosoma brucei to gradual loss of kinetoplast DNA: Trypanosoma equiperdum and Trypanosoma evansi are petite mutants of T. brucei
    De Hua Lai
    Biology Centre, Institute of Parasitology, Czech Academy of Sciences, 37005 Ceske Budejovice, Czech Republic
    Proc Natl Acad Sci U S A 105:1999-2004. 2008
    ..They should be considered two subspecies, respectively T. brucei equiperdum and T. brucei evansi, which spontaneously arose recently. Dk/Ak trypanosomes may potentially emerge repeatedly from T. brucei...
  79. ncbi An EBA175 homologue which is transcribed but not translated in erythrocytic stages of Plasmodium falciparum
    T Triglia
    The Walter and Eliza Hall Institute of Medical Research, Royal Melbourne Hospital, Melbourne, Victoria 3050, Australia
    Mol Biochem Parasitol 116:55-63. 2001
    ..Additionally, it was possible to disrupt the Psi EBA165 gene without affecting the parasite's ability to invade and grow in erythrocytes. These results suggest that the Psi EBA165 gene is a transcribed pseudogene...
  80. ncbi Recent origin of Plasmodium falciparum from a single progenitor
    S K Volkman
    The Harvard-Oxford Malaria Genome Diversity Project, Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, MA 02115, USA
    Science 293:482-4. 2001
    ..These data establish the recent origin of P. falciparum and further provide an explanation for the abundant diversity observed in antigen and other selected genes...
  81. ncbi The transcription machinery and the molecular toolbox to control gene expression in Toxoplasma gondii and other protozoan parasites
    Markus Meissner
    Hygieneinstitut, Abteilung Parasitologie, Universitatsklinikum Heidelberg, Im Neuenheimer Feld 324, D 69120 Heidelberg, Germany
    Microbes Infect 7:1376-84. 2005
    ..These findings have some direct implications on development of tools...
  82. ncbi Mitochondrial remnant organelles of Giardia function in iron-sulphur protein maturation
    Jorge Tovar
    School of Biological Sciences, Royal Holloway, University of London, Egham, Surrey TW20 0EX, UK
    Nature 426:172-6. 2003
    ..Our results indicate that Giardia is not primitively amitochondrial and that it has retained a functional organelle derived from the original mitochondrial endosymbiont...
  83. ncbi Targeting of a tetracycline-inducible expression system to the transcriptionally silent minichromosomes of Trypanosoma brucei
    Bill Wickstead
    School of Biological Sciences, University of Manchester, 2 205 Stopford Building, Oxford Road, Manchester M13 9PT, UK
    Mol Biochem Parasitol 125:211-6. 2002
  84. pmc Genetic structure of the mating-type locus of Chlamydomonas reinhardtii
    Patrick J Ferris
    Department of Biology, Washington University, St Louis, Missouri 63130, USA
    Genetics 160:181-200. 2002
    ..This report extends our original conclusion that the MT locus has incurred high levels of mutational change...
  85. pmc A well-conserved Plasmodium falciparum var gene shows an unusual stage-specific transcript pattern
    Sue A Kyes
    Molecular Parasitology Group, Weatherall Institute of Molecular Medicine, Headington, Oxford OX3 9DS, UK
    Mol Microbiol 48:1339-48. 2003
    ..Although varCSA has been shown to encode a chondroitin sulphate A (CSA)-binding PfEMP1, we find that the presence of full-length varCSA transcripts does not correlate with the CSA-binding phenotype...
  86. ncbi Tetrahymena histone acetyltransferase A: a homolog to yeast Gcn5p linking histone acetylation to gene activation
    J E Brownell
    Department of Biology, University of Rochester, New York, 14627, USA
    Cell 84:843-51. 1996
    ..These findings shed light on the biochemical function of the evolutionarily conserved Gcn5p-Ada complex, directly linking histone acetylation to gene activation, and indicate that histone acetylation is a targeted phenomenon...
  87. ncbi Expression and function of surface proteins in Trypanosoma brucei
    E Pays
    Department of Molecular Biology, University of Brussels 67, Rhode St Genese, Belgium
    Mol Biochem Parasitol 91:3-36. 1998
  88. ncbi Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13
    N Hall
    The Wellcome Trust Sanger Institute, The Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    Nature 419:527-31. 2002
    ..We identify a highly conserved sequence element found in the intergenic region of internal var genes that is not associated with their telomeric counterparts...
  89. pmc Short hairpin RNA-mediated knockdown of protein expression in Entamoeba histolytica
    Alicia S Linford
    Department of Microbiology, University of Virginia, Charlottesville, Virginia, USA
    BMC Microbiol 9:38. 2009
    ..We chose to test the use of RNA interference to knock down gene expression in Entamoeba histolytica...
  90. pmc Multifarious transcriptional regulation of adhesion protein gene ap65-1 by a novel Myb1 protein in the protozoan parasite Trichomonas vaginalis
    Shiou Jeng Ong
    Division of Infectious Diseases, Institute of Biomedical Sciences, Academia Sinica, Taipei 11529, Taiwan
    Eukaryot Cell 5:391-9. 2006
    ..Together, these observations suggest that Myb1 critically regulates multifarious ap65-1 transcription, possibly via differential selection of multiple promoter sites upon environmental changes...
  91. pmc Genetic and biochemical analysis of development in Toxoplasma gondii
    J C Boothroyd
    Department of Microbiology and Immunology, Stanford University School of Medicine, CA 94305 5402, USA
    Philos Trans R Soc Lond B Biol Sci 352:1347-54. 1997
    ..Chief among the techniques used for these studies are genetics and molecular genetics. Recent progress in these areas is described...
  92. pmc Mosaic organization and heterogeneity in frequency of allelic recombination of the Plasmodium vivax merozoite surface protein-1 locus
    Chaturong Putaporntip
    Laboratory of Biology and Department of Mathematics, Osaka Institute of Technology, Osaka 535 8585, Japan
    Proc Natl Acad Sci U S A 99:16348-53. 2002
    ..Meanwhile, highly reduced nucleotide diversity was noted in a region encoding the 19-kDa C-terminal epidermal growth factor-like domain of merozoite surface protein-1, a vaccine candidate...
  93. ncbi Complete genome sequence of the apicomplexan, Cryptosporidium parvum
    Mitchell S Abrahamsen
    Department of Veterinary and Biomedical Science, College of Veterinary Medicine, University of Minnesota, St Paul, MN 55108, USA
    Science 304:441-5. 2004
    ..Elucidation of the core metabolism, including enzymes with high similarities to bacterial and plant counterparts, opens new avenues for drug development...
  94. ncbi A novel Plasmodium falciparum ring stage protein, REX, is located in Maurer's clefts
    Paula L Hawthorne
    University of Queensland, Herston, 4006 QLD, Australia
    Mol Biochem Parasitol 136:181-9. 2004
    ..REX persists in Maurer's clefts throughout the infection of the erythrocyte, where it may play a role in the biogenesis and/or function of this organelle...
  95. ncbi The ves multigene family of B. bovis encodes components of rapid antigenic variation at the infected erythrocyte surface
    D R Allred
    Department of Pathobiology, University of Florida, Gainesville 32611, USA
    Mol Cell 5:153-62. 2000
    ..Variation appears to involve creation and modification or loss of a novel, transcribed copy of the gene...
  96. pmc ParameciumDB: a community resource that integrates the Paramecium tetraurelia genome sequence with genetic data
    Olivier Arnaiz
    Centre de Genetique Moleculaire, CNRS, 91198 Gif sur Yvette, France
    Nucleic Acids Res 35:D439-44. 2007
    ..Future ParameciumDB development plans include coordination of human curation of the near 40 000 gene models by members of the research community...
  97. ncbi Sampling gene diversity across the supergroup Amoebozoa: large EST data sets from Acanthamoeba castellanii, Hartmannella vermiformis, Physarum polycephalum, Hyperamoeba dachnaya and Hyperamoeba sp
    Russell F Watkins
    Department of Biochemistry and Molecular Biology, Dalhousie University, Halifax, Nova Scotia, Canada B3H 1X5
    Protist 159:269-81. 2008
    ....
  98. ncbi A rhoptry-protein-associated mechanism of clonal phenotypic variation in rodent malaria
    P R Preiser
    Division of Parasitology, National Institute for Medical Research, London, UK
    Nature 398:618-22. 1999
    ..This phenomenon is genetically and functionally distinct from classical antigenic variation, which is mediated by the var multigene family of P. falciparum...
  99. pmc Primary structure and expression of the dihydropteroate synthetase gene of Plasmodium falciparum
    T Triglia
    Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia
    Proc Natl Acad Sci U S A 91:7149-53. 1994
    ..We have determined the sequence of the DHPS portion of the gene from sulfadoxine-sensitive and -resistant P. falciparum clones and identified sequence differences that may have a role in sulfone/sulfonamide resistance...
  100. ncbi YakA, a protein kinase required for the transition from growth to development in Dictyostelium
    G M Souza
    Verna and Marrs McLean Department of Biochemistry, Baylor College of Medicine, Houston, Texas 77030, USA
    Development 125:2291-302. 1998
    ..YakA appears to regulate the transition from growth to development in Dictyostelium...
  101. pmc Diverse expression patterns of subgroups of the rif multigene family during Plasmodium falciparum gametocytogenesis
    Michaela Petter
    Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany
    PLoS ONE 3:e3779. 2008
    ..falciparum genome, such as the PfEMP1, STEVOR and RIFIN proteins. While expression of the former two families in sexual stages has been investigated earlier, we report here RIFIN expression during gametocytogenesis...

Research Grants65

  1. HYPOTRICH GENOME IN DEVELOPMENT AND EVOLUTION
    David Prescott; Fiscal Year: 2001
    ..The experiments deal with the general problem of how an organism can manipulate its own DNA. ..
  2. Translational Control in Plasmodium Sexual Development
    Liwang Cui; Fiscal Year: 2005
    ....
  3. DRUG RESISTANCE AND PTERIDINE METABOLISM IN LEISHMANIA
    Stephen M Beverley; Fiscal Year: 2010
    ..These studies have yielded new opportunities for chemotherapeutic attack and new directions for future study of pteridine metabolism and biology. [unreadable] ..
  4. Plasmodium vivax:Proteomic Analysis of Asexual and Sporozoite Stages
    Liwang Cui; Fiscal Year: 2007
    ..Its recent resurgence in prevalence and emergence of drug- resistant parasite strains have raised a great concern for the future control of malaria. [unreadable] [unreadable] [unreadable]..
  5. Gene Duplication and Genome Evolution
    Austin Hughes; Fiscal Year: 2004
    ..5) Examining the role of transposable elements in duplication of genomic segments by testing for nonrandom association between these elements and putatively duplicated blocks in the yeast genome. ..
  6. Development of a cell-based method for high-throughput drug screening in malaria
    Liwang Cui; Fiscal Year: 2008
    ..To facilitate antimalarial drug discovery, we propose to develop a highly sensitive, cell-based, one-step luminescent drug screen assay for high throughput screening of large chemical libraries. [unreadable] [unreadable] [unreadable]..
  7. A new family of secreted adhesive proteins in Plasmodium
    THOMAS TEMPLETON; Fiscal Year: 2008
    ....
  8. EVOLUTION OF IMMUNE RECOGNITION AND EVASION
    Austin L Hughes; Fiscal Year: 2010
    ..In addition, by increasing our understanding of the population diversity and evolutionary history of human immune system genes, this research will enhance our knowledge of human immune function in health and disease. ..
  9. Calcium ATPases in Apicomplexan Parasites as Potential D
    L Sibley; Fiscal Year: 2006
    ..Knowledge gained through these studies may improve the ability to pharmacologically inhibit key calcium regulatory enzymes and hence block motility and invasion by this important group of parasites. ..
  10. Nucleoside Transporters of Plasmodium falciparum
    Buddy Ullman; Fiscal Year: 2007
    ..falciparum in order to test whether PfNT1 function is essential to the intact parasite. We will then characterize the resultant transport and growth phenotypes. ..
  11. R13 travel grant for Polyamine/parasite conference in Portland, OR
    Buddy Ullman; Fiscal Year: 2006
    ..abstract_text> ..
  12. GCN5-mediated transcription in AIDS pathogen Toxoplasma
    WILLIAM SULLIVAN JR; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  13. GENETIC ANALYSIS OF PURINE METABOLISM IN LEISHMANIA DONO
    Buddy Ullman; Fiscal Year: 2007
    ..coli that overexpress hgprt genes, and[unreadable] intact parasites. ..
  14. Mucosal Immunity to Toxoplasma gondii
    L Sibley; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  15. Role of Cryptosporidium Mucins in Host-parasite interactions
    ROBERTA O CONNOR; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  16. GCN5-mediated transcription in AIDS pathogen Toxoplasma
    WILLIAM SULLIVAN JR; Fiscal Year: 2008
    ..Thus, our results will fundamentally advance the fields of parasitology and eukaryotic gene expression. ..
  17. Differentiation in T.cruzi:an emerging AIDS pathogen
    Huan Huang; Fiscal Year: 2009
    ..To further delineate the components of this pathway, we will identify the TcPKA downstream interacting molecules, using both yeast two-hybrid assays and proteomic approaches. ..
  18. Drug resistance in Plasmodium falciparum
    Johanna Daily; Fiscal Year: 2007
    ..abstract_text> ..
  19. Novel Anti-Malarials by Combinatorial Pharmacogenomics
    Joseph DeRisi; Fiscal Year: 2006
    ..The compiled relationships from known anti-malarials, novel quinolines, and toxicology will then be used to guide and optimize additional synthesis efforts toward the ultimate goal of producing effective therapeutics. ..
  20. GENETIC DISSECTION OF TOXOPLASMA GONDII
    David Roos; Fiscal Year: 2008
    ..In addition to their value for the analysis of folate metabolism and drug resistance, these tools should be broadly applicable to other studies on the biology and biochemistry of Toxoplasma. ..
  21. APEs as novel drug targets in AIDS opportunistic pathogen Toxoplasma
    WILLIAM SULLIVAN JR; Fiscal Year: 2008
    ..Moreover, Toxoplasma can be informative as a model organism to study Plasmodium, the causative agent of malaria. [unreadable] [unreadable] [unreadable] [unreadable]..
  22. Polyamine Metabolism in Leishmania
    Buddy Ullman; Fiscal Year: 2008
    ..donovani. We propose, therefore, to ascertain whether the disparity in the abilities of ?lmarg L. mexicana and ?ldodc L. donovani to infect mice can be ascribed to species or gene differences. ..
  23. ZEISS AXIOPLAN 2 IMAGING SYSTEM
    ROBERTA O CONNOR; Fiscal Year: 2005
    ..abstract_text> ..
  24. MECHANISM-BASED DRUG SELECTION AND DESIGN: NUCLEOTIDE SA
    Buddy Ullman; Fiscal Year: 2003
    ..Concurrent experiments will determine how NTPase functions in AK HGXPRT-, and AT parasites, identify the protein(s) which regulate NTPase enzymatic activity, and evaluate the impact of abrogating NTPase expression on parasite viability. ..
  25. Gut Epithelial Cell Receptors for C parvum Invasion
    ROBERTA O CONNOR; Fiscal Year: 2005
    ..New England Medical Center, in association with Tufts University and the wider Boston scientific community provides researchers with diverse and excellent scientific resources, and is exceptional environment for a young scientist...
  26. Confocal Microscope for Microbiology
    L Sibley; Fiscal Year: 2002
    ..This instrument will greatly strengthen the research programs of these highly productive groups by applying high-resolution confocal microscopy to the study of human pathogens...
  27. GENE DISCOVERY IN TOXOPLASMA GONDII
    L Sibley; Fiscal Year: 2003
    ..In combination with the genetic tools available in T. gondii, this will greatly accelerate investigations into the complex developmental biology of apicomplexan parasites. ..
  28. P. falciparum vaccine: Evaluating candidacy of PfMSP3/PfMSP6 in an endemic settin
    Julian Rayner; Fiscal Year: 2008
    ..This data will directly impact vaccine design by allowing us to rule specific sub-domains in or out of the ongoing malaria vaccine development process. [unreadable] [unreadable] [unreadable]..
  29. Conference Proposal to support FASEB Conference on Ciliate Molecular Biology
    Carolyn Price; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  30. MOLECULAR ANALYSIS OF ANTIGENIC VARIATION IN MALARIA
    Mary Galinski; Fiscal Year: 2004
    ....
  31. Resistance of Malaria Parasites to Artemisinin-Based Combination Therapies
    Philip Rosenthal; Fiscal Year: 2008
    ..abstract_text> ..
  32. Differentiation and Signaling in Toxoplasmosis
    Kami Kim; Fiscal Year: 2008
    ..These studies will further elucidate cAMP signaling pathways in T. gondii and determine the role of cAMP signaling pathways during T. gondii differentiation into latent bradyzoites. ..
  33. REGULATION OF DEVELOPMENT BY PHOSPHORELAY SIGNALING
    CHARLES SINGLETON; Fiscal Year: 2002
    ..Finally genetic screens for suppressers or synthetic phenotypes will identify new and perhaps novel components of the regulatory pathway, including upstream inputs, phosphorelay regulators, and downstream effectors. ..
  34. RNA METABOLISM IN TRYPANOSOMES
    Elisabetta Ullu; Fiscal Year: 2009
    ..3. Further characterize the RNAi pathway in terms of its components and how the interaction of TbAGO1 with translating ribosomes is brought about. ..
  35. Fatty Acid Biosynthesis in Cryptosporidium parvum
    Guan Zhu; Fiscal Year: 2010
    ..3) To validate that fatty acid metabolic enzymes may serve as rational drug target in Cryptosporidium by discovering inhibitors selectively against, parasite fatty acid metabolic enzymes. ..
  36. GP46 GENES AND PROTEIN EXPRESSION IN LEISHMANIA CHAGASI
    JOHN DONELSON; Fiscal Year: 2002
    ....
  37. Identification of Tetrahymena DNA Rearrangement Genes
    DOUGLAS LEE CHALKER; Fiscal Year: 2010
    ..abstract_text> ..
  38. Identification of Transcriptional Regulatory Networks in Entamoeba Histolytica
    Upinder Singh; Fiscal Year: 2008
    ..We are interested in understanding the molecular mechanisms that the parasite uses to cause disease. [unreadable] [unreadable] [unreadable]..
  39. Biosynthesis and Function of Base J
    Robert S Sabatini; Fiscal Year: 2010
    ..These studies may prove useful in identifying novel approaches to prevention, treatment and diagnosis of the debilitating and deadly diseases caused by these parasites. ..
  40. CRYPTOSPORIDIUM PARVUM GENOME SEQUENCING PROJECT (MINN)
    Mitchell Abrahamsen; Fiscal Year: 2002
    ..parvum genome. ..
  41. Assessing Genetic Diveristy in Entamoeba Histolytica
    Upinder Singh; Fiscal Year: 2006
    ..This approach will also provide a number of novel genetic targets for the development of improved epidemiological markers, diagnostic assays, and treatment strategies. ..
  42. 2006 Biology of Host-Parasite Interactions Gordon Research Conference
    Elisabetta Ullu; Fiscal Year: 2006
    ..Host-Cell Invasion (Discussion Leader: Barbara Burleigh: Harvard) Trichomonads and Amoebae (Discussion Leader: Patricia Johnson, UCLA) Parasite Membrane Biology (Discussion Leader: Scott Landfear: University of Oregon) ..
  43. Falcipain Cysteine Proteases of Malaria Parasites
    Philip Rosenthal; Fiscal Year: 2005
    ..Experiments designed to accomplish these aims should improve our understanding of the biology of malaria parasites and aid in the characterization of falcipains as potential drug targets. ..
  44. IMPDH as a Drug Target in Cryptosporidium
    Boris Striepen; Fiscal Year: 2008
    ..This work will provide urgently needed molecular reagents to understand the biology of nutrient uptake and drug resistance in this unique parasite. [unreadable] [unreadable] [unreadable]..
  45. Role of cysteine proteases in malaria transmission
    KIM WILLIAMSON; Fiscal Year: 2006
    ..In addition to increasing our understanding of the genes involved, this information can be used to direct the design of cysteine protease inhibitors that will effectively block malaria transmission. ..
  46. Chromosome breakage and DNA detection in Tetrahymena
    Meng Chao Yao; Fiscal Year: 2006
    ..abstract_text> ..
  47. Purine salvage in Cryptosporidium parvum
    Mitchell Abrahamsen; Fiscal Year: 2005
    ..In addition, these studies demonstrate our ability to use heterologous expression of C. parvum genes in yeast as a means to develop a high-throughput screening assay for the identification of specific inhibitors. ..
  48. Development of Assay for High Throughput Screen of Parasite Glucose Transporter
    Scott M Landfear; Fiscal Year: 2010
    ..Since glucose is an essential nutrient for these parasites, such compounds would kill the parasite and could act as novel drugs for treatment of the diseases they cause. ..
  49. VACCINE DEVELOPMENT IN TUBERCULOSIS
    Antonio Campos Neto; Fiscal Year: 2004
    ..In addition to the evaluation of these antigens, we will characterize and clone other vaccine candidates using recently developed approaches for the direct identification of both CD4 and CD8 T cell antigens. ..
  50. Role of Rac and Rac Effectors in Controlling Chemotaxis
    RICHARD FIRTEL; Fiscal Year: 2005
    ....
  51. Ninth International Congress on Toxoplasmosis
    Boris Striepen; Fiscal Year: 2007
    ..gondii that is not often seen in fields as high paced and[unreadable] competitive and has facilitated rapid and sustained progress in research on this pathogen[unreadable] and related parasites. ..
  52. Clinical & Molecular Studies of Drug Resistant Malaria
    Philip Rosenthal; Fiscal Year: 2008
    ..abstract_text> ..
  53. TOXOPLASMA DEVELOPMENT AND CELL CYCLE
    Kami Kim; Fiscal Year: 2002
    ..These will be the initial experiments in a long-term effort to define the role of cell cycle regulation in Toxoplasma tachyzoite-bradyzoite differentiation and virulence. ..
  54. Zinc metalloprotease families in Trypansoma brucei
    JOHN DONELSON; Fiscal Year: 2009
    ....
  55. Gene Structure and Transciptional Regulation
    RICHARD FIRTEL; Fiscal Year: 2007
    ..abstract_text> ..
  56. Assembly, Targeting, And Regulation Of Dynein Motors
    Mary Porter; Fiscal Year: 2004
    ....
  57. Antigen Detection Assay for Tuberculosis
    Antonio Campos Neto; Fiscal Year: 2006
    ..unreadable] [unreadable] [unreadable]..
  58. Function & Regulation of two Novel PARPs, tankyrase 1&2
    Carolyn Price; Fiscal Year: 2008
    ..Aim 4 will investigate the in vivo regulation of tankyrase PARP activity. We will examine the in vivo levels of tankyrase poly(ADP-ribosyl)ation and whether this is regulated by phosphorylation. ..
  59. SERINE PROTEINASES IN APICOMPLEXAN PARASITES
    Kami Kim; Fiscal Year: 2009
    ..In addition to illuminating the function and biology of T. gondii secretory organelles, these studies will determine whether subtilases are a viable chemotherapeutic target for treatment of human infections with Apicomplexan parasites. ..
  60. Overexpression of membrane proteins in Tetrahymena
    THEODORE CLARK; Fiscal Year: 2006
    ..The technology described here is intended to better understand how these proteins function, leading ultimately to strategies for the treatment of serious disorders ranging from cancer to stoke. [unreadable] [unreadable]..
  61. Forward Genetics in Tetrahymena: Cloning by Complementation
    ROBERT COYNE; Fiscal Year: 2008
    ..Numerous other basic studies with far-reaching relevance to human health would be facilitated by the availability of the proposed freely available resource. [unreadable] [unreadable] [unreadable] [unreadable]..
  62. EXPRESSION OF THE LEISHMANIA TARENTOLAE SL RNA GENE
    David Campbell; Fiscal Year: 2003
    ..These unique features represent potential targets for selective therapeutic intervention. ..
  63. RNAi in DNA Sequence Elimination in Tetrahymena
    Martin A Gorovsky; Fiscal Year: 2010
    ..These studies should shed light on other RNAi- like processes that are important for normal development and maintenance of genome stability in multicellular organisms. ..
  64. "Vivax Malaria Research: 2005 and Beyond"
    Jane Carlton; Fiscal Year: 2005
    ..vivax with P. falciparum, epidemiology and diversity studies, vectors and transmission dynamics, and a bioinformatics workshop for researchers and students to learn to manipulate Plasmodium sequence data. ..
  65. Comparative Evolutionary Genomics and Infectious Disease
    Jane Carlton; Fiscal Year: 2010
    ....