Genomes and Genes
Summary: The complete genetic complement contained in a set of CHROMOSOMES in a protozoan (PROTOZOA).
Publications216 found, 100 shown here
- Analysis of a piwi-related gene implicates small RNAs in genome rearrangement in tetrahymenaKazufumi Mochizuki
Department of Biology, University of Rochester, Rochester, NY 14627, USA
Cell 110:689-99. 2002..We propose that these small RNAs function to specify sequences to be eliminated by a mechanism similar to RNA-mediated gene silencing...
- Comparative genomics of trypanosomatid parasitic protozoaNajib M El-Sayed
Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA
Science 309:404-9. 2005..Contrary to recent reports, our analyses reveal no evidence that these species are descended from an ancestor that contained a photosynthetic endosymbiont...
- The genome of the African trypanosome Trypanosoma bruceiMatthew Berriman
Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton CB10 1SA, UK
Science 309:416-22. 2005..brucei and the greatest in L. major. Horizontal transfer of genes of bacterial origin has contributed to some of the metabolic differences in these parasites, and a number of novel potential drug targets have been identified...
- Genome sequence of the human malaria parasite Plasmodium falciparumMalcolm J Gardner
The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, Maryland 20850, USA
Nature 419:498-511. 2002..The genome sequence provides the foundation for future studies of this organism, and is being exploited in the search for new drugs and vaccines to fight malaria...
- Genetic diversity and chloroquine selective sweeps in Plasmodium falciparumJohn C Wootton
Computational Biology Branch, National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, Maryland 20894 6075, USA
Nature 418:320-3. 2002..The presence of linkage disequilibrium provides a basis for mapping genes under drug selection in P. falciparum...
- GiardiaDB and TrichDB: integrated genomic resources for the eukaryotic protist pathogens Giardia lamblia and Trichomonas vaginalisCristina Aurrecoechea
Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, GA 30602, USA
Nucleic Acids Res 37:D526-30. 2009..Phylogenetic relationships may also be interrogated. The ability to combine the results from independent queries, and to store queries and query results for future use facilitates complex, genome-wide mining of functional genomic data...
- Genome of the host-cell transforming parasite Theileria annulata compared with T. parvaArnab Pain
Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
Science 309:131-3. 2005....
- The transcriptome of the intraerythrocytic developmental cycle of Plasmodium falciparumZbynek Bozdech
Department of Biochemistry and Biophysics, University of California, San Francisco, USA
PLoS Biol 1:E5. 2003..falciparum and provide a resource for the identification of new chemotherapeutic and vaccine candidates...
- Genome-wide gene expression profiling analysis of Leishmania major and Leishmania infantum developmental stages reveals substantial differences between the two speciesAnnie Rochette
Research Centre in Infectious Diseases, CHUL Research Centre and Department of Medical Biology, Faculty of Medicine, Laval University, Quebec, Canada
BMC Genomics 9:255. 2008....
- Draft genome sequence of the sexually transmitted pathogen Trichomonas vaginalisJane M Carlton
Institute for Genomic Research, 9712 Medical Research Drive, Rockville, MD 20850, USA
Science 315:207-12. 2007..The genome sequence predicts previously unknown functions for the hydrogenosome, which support a common evolutionary origin of this unusual organelle with mitochondria...
- The genome sequence of Trypanosoma cruzi, etiologic agent of Chagas diseaseNajib M El-Sayed
Department of Parasite Genomics, Institute for Genomic Research, Rockville, MD 20850, USA
Science 309:409-15. 2005....
- Comparative genomics of the neglected human malaria parasite Plasmodium vivaxJane M Carlton
The Institute for Genomic Research J Craig Venter Institute, 9704 Medical Research Drive, Rockville, Maryland 20850, USA
Nature 455:757-63. 2008..Completion of the P. vivax genome provides the scientific community with a valuable resource that can be used to advance investigation into this neglected species...
- dictyBase: a new Dictyostelium discoideum genome databaseLisa Kreppel
Laboratory of Cellular and Developmental Biology 50 3351, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892 8028, USA
Nucleic Acids Res 32:D332-3. 2004....
- Genomic minimalism in the early diverging intestinal parasite Giardia lambliaHilary G Morrison
Marine Biological Laboratory, Woods Hole, MA 02543 1015, USA
Science 317:1921-6. 2007..This genome sequence will not only be valuable for investigating the evolution of eukaryotes, but will also be applied to the search for new therapeutics for this parasite...
- A three-genome phylogeny of malaria parasites (Plasmodium and closely related genera): evolution of life-history traits and host switchesEllen S Martinsen
Department of Biology, University of Vermont, Burlington, VT 05405, USA
Mol Phylogenet Evol 47:261-73. 2008..The Plasmodium of birds and squamate reptiles all fall within a single clade, with evidence for repeated switching between birds and squamate hosts...
- The genome of the protist parasite Entamoeba histolyticaBrendan Loftus
TIGR, 9712 Medical Center Drive, Rockville, Maryland 20850, USA
Nature 433:865-8. 2005..Analysis of the genome provides new insights into the workings and genome evolution of a major human pathogen...
- ToxoDB: an integrated Toxoplasma gondii database resourceBindu Gajria
Department of Biology, Department of Genetics, Center for Bioinformatics, University of Pennsylvania, Philadelphia, PA, USA
Nucleic Acids Res 36:D553-6. 2008..ToxoDB has matured significantly since its initial release. Here we outline the numerous updates with respect to the data and increased functionality available on the website...
- Global trends of whole-genome duplications revealed by the ciliate Paramecium tetraureliaJean Marc Aury
Genoscope and CNRS UMR 8030, 2 rue Gaston Crémieux CP5706, 91057 Evry, France
Nature 444:171-8. 2006..The conclusion of this analysis is that many genes are maintained after whole-genome duplication not because of functional innovation but because of gene dosage constraints...
- Selective upregulation of a single distinctly structured var gene in chondroitin sulphate A-adhering Plasmodium falciparum involved in pregnancy-associated malariaAli Salanti
Centre for Medical Parasitology at the Institute for Medical Microbiology and Immunology, University of Copenhagen, Panum Institute 24 2, and Department of Infectious Diseases, Copenhagen University Hospital Rigshospitalet, Denmark
Mol Microbiol 49:179-91. 2003..falciparum malaria. This var gene thus has the properties expected of a gene encoding the parasite adhesion molecule that initiates the pathology associated with PAM...
- Composite genome map and recombination parameters derived from three archetypal lineages of Toxoplasma gondiiAsis Khan
Department of Molecular Microbiology, Center for Infectious Diseases, Washington University School of Medicine St Louis, MO 63110, USA
Nucleic Acids Res 33:2980-92. 2005..The resulting genome map provides a framework for analysis of complex traits such as virulence and transmission, and for comparative population genetic studies...
- Macronuclear genome sequence of the ciliate Tetrahymena thermophila, a model eukaryoteJonathan A Eisen
The Institute for Genomic Research, Rockville, Maryland, United States of America
PLoS Biol 4:e286. 2006..thermophila an ideal model for functional genomic studies to address biological, biomedical, and biotechnological questions of fundamental importance...
- Four histone variants mark the boundaries of polycistronic transcription units in Trypanosoma bruceiT Nicolai Siegel
Laboratory of Molecular Parasitology, The Rockefeller University, New York, New York 10065, USA
Genes Dev 23:1063-76. 2009....
- Tetrahymena Genome Database (TGD): a new genomic resource for Tetrahymena thermophila researchNicholas A Stover
Department of Genetics, School of Medicine, Stanford University, Stanford, CA 94305 5120, USA
Nucleic Acids Res 34:D500-3. 2006..A variety of tools are available at TGD for searching the Tetrahymena genome, its literature and information about members of the research community...
- Germ line transcripts are processed by a Dicer-like protein that is essential for developmentally programmed genome rearrangements of Tetrahymena thermophilaColin D Malone
Biology Department, Washington University, St Louis, MO 63130, USA
Mol Cell Biol 25:9151-64. 2005....
- The Trypanosoma cruzi proteomeJ A Atwood
Complex Carbohydrate Research Center, University of Georgia, Athens, GA 30602, USA
Science 309:473-6. 2005..The four parasite stages appear to use distinct energy sources, including histidine for stages present in the insect vectors and fatty acids by intracellular amastigotes...
- Comparative analysis of the kinomes of three pathogenic trypanosomatids: Leishmania major, Trypanosoma brucei and Trypanosoma cruziMarilyn Parsons
Seattle Biomedical Research Institute, Seattle, WA 98109, USA
BMC Genomics 6:127. 2005..As a part of an effort to understand parasite signaling functions, we report the results of a genome-wide analysis of protein kinases (PKs) of these three trypanosomatids...
- Evidence for multiple hybrid groups in Trypanosoma cruziNancy R Sturm
Department of Microbiology, Immunology and Molecular Genetics, University of California at Los Angeles, 609 Charles E Young Drive East, Los Angeles, CA 90095 1489, USA
Int J Parasitol 33:269-79. 2003..These data demonstrate for the first time that IIa/IIc are hybrid, consistent with the hypothesis that genetic recombination has occurred more than once within the T. cruzi lines...
- A functional role for transposases in a large eukaryotic genomeMariusz Nowacki
Department of Ecology and Evolutionary Biology, Princeton University, Princeton, NJ 08544, USA
Science 324:935-8. 2009..This study suggests a new important role in Oxytricha for this large portion of genomic DNA that was previously thought of as junk...
- The genetic map and comparative analysis with the physical map of Trypanosoma bruceiAnnette MacLeod
Wellcome Centre for Molecular Parasitology, Anderson College Complex, University of Glasgow, 56 Dumbarton Road, Glasgow G11 6NU, UK
Nucleic Acids Res 33:6688-93. 2005....
- Cryptosporidium parvum genes containing thrombospondin type 1 domainsMingqi Deng
Department of Veterinary Pathobiology, University of Minnesota, St Paul 55108, USA
Infect Immun 70:6987-95. 2002..Only CpTSP4 was highly expressed solely at an early stage of infection...
- Whole-genome comparative RNA expression profiling of axenic and intracellular amastigote forms of Leishmania infantumAnnie Rochette
Research Centre in Infectious Disease, CHUL Research Centre, Quebec, Canada G1V 4G2
Mol Biochem Parasitol 165:32-47. 2009..These findings highlight the importance of the host macrophage in driving the parasite to specific adaptations, which consequently result in highly regulated changes in gene expression...
- Identification of Plasmodium falciparum antigens by antigenic analysis of genomic and proteomic dataDenise L Doolan
Malaria Program, Naval Medical Research Center, Silver Spring, MD 20910 7500, USA
Proc Natl Acad Sci U S A 100:9952-7. 2003..The data suggest that immune responses to Plasmodium are dispersed on a relatively large number of parasite antigens. These studies have implications for our understanding of immunodominance and breadth of responses to complex pathogens...
- Genome sequence and gene compaction of the eukaryote parasite Encephalitozoon cuniculiM D Katinka
Genoscope, UMR CNRS 8030, CP 5706, 91057 Evry Cedex, France
Nature 414:450-3. 2001..Because the E. cuniculi genome contains genes related to some mitochondrial functions (for example, Fe-S cluster assembly), we hypothesize that microsporidia have retained a mitochondrion-derived organelle...
- The steady-state transcriptome of the four major life-cycle stages of Trypanosoma cruziTodd A Minning
Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, Georgia 30602, USA
BMC Genomics 10:370. 2009..cruzi and related kinetoplastid parasites have suggested that the transcript abundances of most genes in these organisms do not vary significantly between life-cycle stages...
- From genome to vaccines for leishmaniasis: screening 100 novel vaccine candidates against murine Leishmania major infectionCarmel B Stober
Cambridge Institute for Medical Research, University of Cambridge, Cambridge CB2 2XY, UK
Vaccine 24:2602-16. 2006..Two ribosomal proteins, a V-ATPase subunit, and a dynein light chain orthologue were the only other protective genes with putative functions...
- The genome of the simian and human malaria parasite Plasmodium knowlesiA Pain
Wellcome Trust Sanger Institute, Genome Campus, Hinxton, Cambridgeshire CB10 1SA, UK
Nature 455:799-803. 2008..One of these families, the KIRs, contains sequences that collectively match over one-half of the host CD99 extracellular domain, which may represent an unusual form of molecular mimicry...
- GOtcha: a new method for prediction of protein function assessed by the annotation of seven genomesDavid M A Martin
Post Genomics and Molecular Interactions Centre, School of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, UK
BMC Bioinformatics 5:178. 2004..GOtcha predicts GO term associations with term-specific probability (P-score) measures of confidence. Term-specific probabilities are a novel feature of GOtcha and allow the identification of conflicts or uncertainty in annotation...
- Two families of rep-like genes that probably originated by interspecies recombination are represented in viral, plasmid, bacterial, and parasitic protozoan genomesMark J Gibbs
Mol Biol Evol 23:1097-100. 2006....
- Functional genomic characterization of mRNAs associated with TcPUF6, a pumilio-like protein from Trypanosoma cruziBruno Dallagiovanna
Instituto de Biologia Molecular do Paraná, Rua Professor Algacyr Munhoz Mader 3775, Curitiba, PR, Brazil
J Biol Chem 283:8266-73. 2008..Our results suggest that TcPUF6 regulates the half-lives of its associated transcripts via differential association with mRNA degradation complexes throughout its life cycle...
- JBP1 and JBP2 are two distinct thymidine hydroxylases involved in J biosynthesis in genomic DNA of African trypanosomesLaura J Cliffe
Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia 30602, USA
Nucleic Acids Res 37:1452-62. 2009..Reintroduction of JBP2 in the J-null trypanosome stimulates HOMedU formation and site-specific synthesis of base J. We conclude that JBP2 and JBP1 are the TH enzymes involved in J biosynthesis...
- A phylogenomic inventory of meiotic genes; evidence for sex in Giardia and an early eukaryotic origin of meiosisMarilee A Ramesh
Department of Biology, Roanoke College, Salem, VA 24153, USA
Curr Biol 15:185-91. 2005....
- Host switch leads to emergence of Plasmodium vivax malaria in humansJianbing Mu
Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, Maryland, USA
Mol Biol Evol 22:1686-93. 2005..Taken together, a haplotype network, parasite migration patterns, demographic history, and cophylogeny mapping support an Asian origin via a host switch from macaque monkeys...
- The LINEs and SINEs of Entamoeba histolytica: comparative analysis and genomic distributionAbhijeet A Bakre
School of Environmental Sciences, Jawaharlal Nehru University, New Delhi 110067, India
Exp Parasitol 110:207-13. 2005..The elements were very frequently located close to protein-coding genes and other EhLINEs/SINEs. The possible influence of these elements on expression of neighboring genes needs to be determined...
- Reassessment of the epidemiology of amebiasis: state of the artCecilia Ximenez
Departamento de Medicina Experimental, Facultad de Medicina, Universidad Nacional Autonoma de Mexico, Mexico DF, Mexico
Infect Genet Evol 9:1023-32. 2009..Finally, we will discuss those areas of the host-parasite relationship that are still not fully understood, and the scientific challenges to approach this important public health problem in the future...
- The genome of the kinetoplastid parasite, Leishmania majorAlasdair C Ivens
Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire CB10 1SA, UK
Science 309:436-42. 2005..Abundant RNA-binding proteins are encoded in the Tritryp genomes, consistent with active posttranscriptional regulation of gene expression...
- Detecting selection using a single genome sequence of M. tuberculosis and P. falciparumJoshua B Plotkin
Harvard Society of Fellows and Bauer Center for Genomics Research, 7 Divinity Avenue, Cambridge, Massachusetts 02138, USA
Nature 428:942-5. 2004..Our method of estimating selective pressures requires far fewer data than comparative sequence analysis, and it measures selection across an entire genome; the method can readily be applied to a large range of sequenced organisms...
- Molecular genetics and comparative genomics reveal RNAi is not functional in malaria parasitesJake Baum
The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria 3052, Australia
Nucleic Acids Res 37:3788-98. 2009....
- Leishmania: conserved evolution--diverse diseasesMiriam A Lynn
Immunity and Infection Research Centre, Vancouver Coastal Health Institute, and the Department of Medical Genetics, University of British Columbia, 2660 Oak Street, Vancouver, V6H 3Z6 BC, Canada
Trends Parasitol 24:103-5. 2008..Here, we discuss these findings together with recent microarray and proteomics studies and highlight their importance in understanding Leishmania disease phenotypes...
- Genome-wide characterization of tetrahymena thermophila chromosome breakage sites. I. Cloning and identification of functional sitesEileen Hamilton
Department of Molecular, Cellular and Developmental Biology, University of California, Santa Barbara, California 93106, USA
Genetics 170:1611-21. 2005....
- Large-scale genome remodelling by the developmentally programmed elimination of germ line sequences in the ciliate ParameciumMireille Bétermier
CNRS UMR 8541, Laboratoire de Genetique Moleculaire, Ecole Normale Superieure, 46 rue d Ulm, 75005 Paris, France
Res Microbiol 155:399-408. 2004..Recent data point to a role of non-coding RNAs in the epigenetic programming of these rearrangements...
- Kinetoplastids: related protozoan pathogens, different diseasesKen Stuart
Seattle Biomedical Research Institute, Seattle, Washington, USA
J Clin Invest 118:1301-10. 2008....
- Initiator and upstream elements in the alpha2-tubulin promoter of Giardia lambliaH G Elmendorf
Laboratory of Parasitic Diseases, NIH, Bethesda, MD 20892 0425, USA
Mol Biochem Parasitol 113:157-69. 2001..In particular, the short and diverse sequences that function in transcription initiation in Giardia suggest the potential for relaxed transcriptional regulation...
- Autophagy during proliferation and encystation in the protozoan parasite Entamoeba invadensKarina Picazarri
Department of Parasitology, Gunma University Graduate School of Medicine, 3 39 22 Showa machi, Maebashi, Gunma 371 8511, Japan
Infect Immun 76:278-88. 2008..invadens. These results suggest that the core machinery for autophagy is conserved and plays an important role during proliferation and differentiation in Entamoeba...
- Sequence of Plasmodium falciparum chromosome 12Richard W Hyman
Stanford Genome Technology Center, 855 California Avenue, Palo Alto, California 94304, USA
Nature 419:534-7. 2002..We discuss the methodology that yielded a finished and fully contiguous sequence for chromosome 12. The biological implications of the sequence data are more thoroughly discussed in an accompanying Article (ref. 3)...
- Evolution of tubulin gene arrays in Trypanosomatid parasites: genomic restructuring in LeishmaniaAndrew P Jackson
Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire, CB10 1SA, UK
BMC Genomics 7:261. 2006..brucei. Polycistronic transcription in these organisms makes the chromosomal arrangement of tubulin genes important with respect to gene expression...
- Functional genomics in Dictyostelium: MidA, a new conserved protein, is required for mitochondrial function and developmentPatricia Torija
Instituto de Investigaciones Biomédicas Alberto Sols C S I C U A M, Calle Arturo Duperier 4, 28029 Madrid, Spain
J Cell Sci 119:1154-64. 2006..This work exemplifies the potential of Dictyostelium as a model system for functional genomic studies...
- Molecular characterization of serine-, alanine-, and proline-rich proteins of Trypanosoma cruzi and their possible role in host cell infectionRenata C P Baida
Department of Microbiology, Immunology and Parasitology, Escola Paulista de Medicina, UNIFESP, Rua Botucatu, 862, CEP 04023 062, Sao Paulo, Brazil
Infect Immun 74:1537-46. 2006....
- Rapid turnover of Plasmodium falciparum var gene transcripts and genotypes during natural non-symptomatic infectionsGerhard Wunderlich
Departamento de Parasitologia, Instituto de Ciências Biomédicas 2, Universidade de Sao Paulo, 05508 900 Sao Paulo, SP, Brazil
Rev Inst Med Trop Sao Paulo 47:195-201. 2005....
- Gene synteny and evolution of genome architecture in trypanosomatidsElodie Ghedin
Parasity Genomics, The Institute for Genomics Research, 9712 Medical Center Dr Rockville, MD 20850, USA
Mol Biochem Parasitol 134:183-91. 2004..major by examining the regions near breakage points of the synteny. This is the first such element found in L. major suggesting that retroelements were found in the common ancestor of all three species...
- A spliceosomal intron in Giardia lambliaJulie E J Nixon
Department of Immunology and Infectious Diseases, Harvard School of Public Health, 665 Huntington Avenue, Boston, MA 02115 6018, USA
Proc Natl Acad Sci U S A 99:3701-5. 2002..If introns were added during eukaryotic evolution (the introns-late hypothesis), then these results push back the date of this event before the branching of G. lamblia...
- A novel family of mobile genetic elements is limited to the germline genome in Tetrahymena thermophilaJeffrey D Wuitschick
Department of Biology, Marquette University, Milwaukee, WI 53201 1881, USA
Nucleic Acids Res 30:2524-37. 2002..Possible mechanisms of developmentally programmed Tlr elimination are discussed...
- Genome sequence of Theileria parva, a bovine pathogen that transforms lymphocytesMalcolm J Gardner
Institute for Genomic Research TIGR, 9712 Medical Center Drive, Rockville, MD 20850, USA
Science 309:134-7. 2005..Several biosynthetic pathways are incomplete or absent, suggesting substantial metabolic dependence on the host cell. One protein family that may generate parasite antigenic diversity is not telomere-associated...
- Structure and content of the Entamoeba histolytica genomeC G Clark
Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London WC1E 7HT, UK
Adv Parasitol 65:51-190. 2007..In this chapter we summarise the features of the genome as they are currently understood and provide previously unpublished analyses of many of the genes...
- Integrated mapping, chromosomal sequencing and sequence analysis of Cryptosporidium parvumAlan T Bankier
Medical Research Council MRC Laboratory of Molecular Biology, Cambridge CB 2 2QH, UK
Genome Res 13:1787-99. 2003..parvum, whereas the other is shared with (but has previously gone unnoticed in) all known genomes of the Coccidia and Haemosporida. These motifs appear to be unique in their structure, distribution and sequences...
- TriTrypDB: a functional genomic resource for the TrypanosomatidaeMartin Aslett
Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire CB10 1SA, UK
Nucleic Acids Res 38:D457-62. 2010....
- The Eimeria genome projects: a sequence of eventsMartin W Shirley
Institute for Animal Health, Compton Laboratory, Compton, Nr Newbury, Berkshire RG20 7NN, UK
Trends Parasitol 20:199-201. 2004
- Population genomics of the immune evasion (var) genes of Plasmodium falciparumAlyssa E Barry
Peter Medawar Building for Pathogen Research, University of Oxford, Oxford, United Kingdom
PLoS Pathog 3:e34. 2007..This population genomic framework provides a cost effective molecular epidemiological tool to rapidly explore the geographic diversity of var genes...
- Ancestral genomes, sex, and the population structure of Trypanosoma cruziJorge M de Freitas
Departamento de Bioquimica e Imunologia, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil
PLoS Pathog 2:e24. 2006..cruzi II and T. cruzi III produced evolutionarily viable progeny. In both events, the mitochondrial recipient (as identified by the mitochondrial clade of the hybrid strains) was T. cruzi II and the mitochondrial donor was T. cruzi III...
- Evidence for the importance of genetic structuring to the structural and functional specialization of the Plasmodium falciparum var gene familySusan M Kraemer
Seattle Biomedical Research Institute, 4 Nickerson Street, Seattle, WA 98109, USA
Mol Microbiol 50:1527-38. 2003....
- The genome of Entamoeba histolyticaA Bhattacharya
School of Life Sciences, Jawaharlal Nehru University, New Mehrauli Road, New Delhi, India
Int J Parasitol 30:401-10. 2000..The Expressed Sequence Tag approach has also helped identify several important genes which encode proteins that may be involved in different biochemical pathways, signal transduction mechanisms and organellar functions...
- Sequence of Plasmodium falciparum chromosomes 2, 10, 11 and 14Malcolm J Gardner
The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, Maryland 20850, USA
Nature 419:531-4. 2002..We report here the nucleotide sequences of chromosomes 10, 11 and 14, and a re-analysis of the chromosome 2 sequence. These chromosomes represent about 35% of the 23-megabase P. falciparum genome...
- Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13N Hall
The Wellcome Trust Sanger Institute, The Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
Nature 419:527-31. 2002..We identify a highly conserved sequence element found in the intergenic region of internal var genes that is not associated with their telomeric counterparts...
- The Plasmodium vivax genome sequencing projectJane Carlton
The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA
Trends Parasitol 19:227-31. 2003..Here, an update on the Plasmodium vivax genome sequencing project is presented, as part of the Trends in Parasitology series of reviews expanding on various aspects of P. vivax research...
- Utility of the Trypanosoma cruzi sequence database for identification of potential vaccine candidates by in silico and in vitro screeningVandanajay Bhatia
Department of Microbiology and Immunology, University of Texas Medical Branch, 301 University Blvd, Galveston 77555, USA
Infect Immun 72:6245-54. 2004..cruzi. Taken together, our results validate the applicability of bioinformatics in genome mining, resulting in the identification of T. cruzi membrane-associated proteins that are potential vaccine candidates...
- Robust method for proteome analysis by MS/MS using an entire translated genome: demonstration on the ciliome of Tetrahymena thermophilaJeffrey C Smith
Department of Chemistry and Centre for Research in Mass Spectrometry, York University, 4700 Keele Street, Toronto, Ontario, Canada M3J 1P3
J Proteome Res 4:909-19. 2005..This methodology allows identifying the locations of the genes encoding these novel proteins, which is a necessary first step to downstream functional genomic experimentation...
- Trichomonas vaginalis surface proteins: a view from the genomeR P Hirt
Institute for Cellular and Molecular Biosciences, Newcastle University, Newcastle upon Tyne, UK
Trends Parasitol 23:540-7. 2007..Here, we review the existing data on T. vaginalis surface proteins and summarize some of the main findings from the recent in silico characterization of its candidate surface proteins...
- Complete genome sequence of the apicomplexan, Cryptosporidium parvumMitchell S Abrahamsen
Department of Veterinary and Biomedical Science, College of Veterinary Medicine, University of Minnesota, St Paul, MN 55108, USA
Science 304:441-5. 2004..Elucidation of the core metabolism, including enzymes with high similarities to bacterial and plant counterparts, opens new avenues for drug development...
- tRNA gene sequences are required for transcriptional silencing in Entamoeba histolyticaHenriette Irmer
Grisebachstr 8, Institute of Microbiology and Genetics, Department of Molecular Microbiology and Genetics, Georg August University, Gottingen, 37077 Gottingen, Germany
Eukaryot Cell 9:306-14. 2010..histolytica promoter, opening a way toward future silencing of individual genes of interest in E. histolytica. Our results indicate that tRNA gene-mediated silencing is not restricted to Saccharomyces cerevisiae...
- A mechanism for cross-resistance to nifurtimox and benznidazole in trypanosomesShane R Wilkinson
School of Biological and Chemical Sciences, Queen Mary University of London, London E1 4NS, United Kingdom
Proc Natl Acad Sci U S A 105:5022-7. 2008..This potential for drug resistance by a simple mechanism has important implications, because nifurtimox is currently undergoing phase III clinical trials against African trypanosomiasis...
- An expanded inventory of conserved meiotic genes provides evidence for sex in Trichomonas vaginalisShehre Banoo Malik
Department of Biology, Roy J Carver Center for Comparative Genomics, University of Iowa, Iowa City, Iowa, United States of America
PLoS ONE 3:e2879. 2008....
- ParameciumDB: a community resource that integrates the Paramecium tetraurelia genome sequence with genetic dataOlivier Arnaiz
Centre de Genetique Moleculaire, CNRS, 91198 Gif sur Yvette, France
Nucleic Acids Res 35:D439-44. 2007..Future ParameciumDB development plans include coordination of human curation of the near 40 000 gene models by members of the research community...
- Sampling gene diversity across the supergroup Amoebozoa: large EST data sets from Acanthamoeba castellanii, Hartmannella vermiformis, Physarum polycephalum, Hyperamoeba dachnaya and Hyperamoeba spRussell F Watkins
Department of Biochemistry and Molecular Biology, Dalhousie University, Halifax, Nova Scotia, Canada B3H 1X5
Protist 159:269-81. 2008....
- Annexin-like alpha giardins: a new cytoskeletal gene family in Giardia lambliaMalin E L Weiland
Microbiology and Tumor Biology Center, Karolinska Institutet, Stockholm, Sweden
Int J Parasitol 35:617-26. 2005..These localisation experiments suggest alpha-giardins play a role in cell motility, attachment and membrane stability...
- A proteomic view of the Plasmodium falciparum life cycleLaurence Florens
Department of Cell Biology, The Scripps Research Institute, SR 11, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
Nature 419:520-6. 2002..The detection of chromosomal clusters encoding co-expressed proteins suggested a potential mechanism for controlling gene expression...
- Hemizygous subtelomeres of an African trypanosome chromosome may account for over 75% of chromosome lengthSergio Callejas
Department of Pathology, University of Cambridge, CB2 1QP, Cambridge, United Kingdom
Genome Res 16:1109-18. 2006..Further experimentation is required to determine if these results are applicable to all size-variable chromosomes...
- The Giardia lamblia genomeR D Adam
Department of Microbiology Immunology and Medicine, University of Arizona College of Medicine, 1501 N Campbell, Tucson 85724, USA
Int J Parasitol 30:475-84. 2000..The results will facilitate a better understanding of the biology of Giardia spp. as well as their phylogenetic relationship to other primitive organisms...
- Cryptosporidium parvum appears to lack a plastid genomeG Zhu
Wadsworth Center, New York State Department of Health, Albany, NY 12201 2002, USA
Microbiology 146:315-21. 2000..It also suggests that unlike the situation with other diseases caused by members of the Apicomplexa, drug development against cryptosporidiosis targeting a plastid genome or metabolic pathways associated with it may not be useful...
- Gene encoding erythrocyte binding ligand linked to blood stage multiplication rate phenotype in Plasmodium yoelii yoeliiSittiporn Pattaradilokrat
Institute of Immunology and Infection Research, School of Biological Sciences, University of Edinburgh, West Mains Road, Edinburgh EH9 3JT, United Kingdom
Proc Natl Acad Sci U S A 106:7161-6. 2009..Our genome-wide LGS analysis also indicated effects of at least 1 other locus on multiplication rate, as did the findings of Otsuki and colleagues on virulence in P. y. yoelii...
- A novel palmitoyl acyl transferase controls surface protein palmitoylation and cytotoxicity in Giardia lambliaMaria C Touz
Laboratory of Parasitic Diseases, NIAID, NIH, Bethesda, MD 20892, USA
Mol Microbiol 58:999-1011. 2005..These results show that VSPs are palmitoylated at the cysteine within the conserved tail by gPAT and indicate an essential function of palmitoylation in control of VSP-mediated signalling and processing...
- Numerous, robust genetic markers for Plasmodium chabaudi by the method of amplified fragment length polymorphismKatrina Grech
University of Edinburgh, Division of Biological Sciences, ICAPB, Ashworth Laboratory, West Mains Road, Edinburgh EH9 3JT, UK
Mol Biochem Parasitol 123:95-104. 2002..6 x 10(-4) per base pair. This is consistent with the sequence diversity found between alleles of candidate drug resistance genes from P. c. chabaudi clones AS and AJ identified and sequenced in this laboratory...
- Fragmentation of mitochondrial large subunit rRNA in the dinoflagellate Alexandrium catenella and the evolution of rRNA structure in alveolate mitochondriaRyoma Kamikawa
Laboratory of Marine Microbiology, Division of Applied Biosciences, Department of Agriculture, Kyoto University, Kyoto 606 8502, Japan
Protist 158:239-45. 2007..However, current sequence data on dinoflagellate mitochondria are insufficient to reject the alternative scenario, in which the rRNA fragmentation evolved independently in apicomplexan and dinoflagellate mitochondria...
- Malaria: new vaccines for old?Andrew Waters
Department of Parasitology, Leiden University Medical Centre, 2333ZA Leiden, The Netherlands
Cell 124:689-93. 2006..Detailed analyses of the 5500 genes revealed by the complete Plasmodium genome sequence are yielding new candidate parasite antigens and strategies that may contribute to a successful vaccine against malaria in the coming decade...
- Differential expression of Leishmania major beta-tubulin genes during the acquisition of promastigote infectivityR M Coulson
Department of Pathology, University of Cambridge, UK
Mol Biochem Parasitol 82:227-36. 1996..These data demonstrate that the regulation of mRNA half-life contributes to controlling gene expression as promastigotes differentiate into an infectious form...
- Inconsistencies of genome annotations in apicomplexan parasites revealed by 5'-end-one-pass and full-length sequences of oligo-capped cDNAsHiroyuki Wakaguri
Department of Medical Genome Sciences, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwanoha, Kashiwa, Chiba, Japan
BMC Genomics 10:312. 2009..To improve upon the current genome annotations, as well as to validate the importance for physical cDNA clone resources, we generated a large-scale collection of full-length cDNAs for several apicomplexa parasites...
- A family affair: var genes, PfEMP1 binding, and malaria diseaseSusan M Kraemer
Seattle Biomedical Research Institute, Seattle, WA 98109 5219, USA
Curr Opin Microbiol 9:374-80. 2006..Detailed understanding of PfEMP1 expression and receptor binding mechanisms during infection and of the antigenic relatedness of disease variants might lead to new approaches in prevention of malaria disease...
- Refined annotation and assembly of the Tetrahymena thermophila genome sequence through EST analysis, comparative genomic hybridization, and targeted gap closureRobert S Coyne
J Craig Venter Institute formerly The Institute for Genomic Research, 9704 Medical Center Dr, Rockville, MD, USA
BMC Genomics 9:562. 2008....
- Early origin and recent expansion of Plasmodium falciparumDeirdre A Joy
Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892 0425, USA
Science 300:318-21. 2003..However, both the world and some regional populations appear to be older (50,000 to 100,000 years old), suggesting an earlier wave of migration out of Africa, perhaps during the Pleistocene migration of human beings...
- Systems biology in malaria researchMartin J Fraunholz
Junior Group Applied Bioinformatics and Cell Biology, Institute of Microbiology, Ernst Moritz Arndt University, Friedrich Ludwig Jahn Strasse 15, D 17487 Greifswald, Germany
Trends Parasitol 21:393-5. 2005..By integrating genomics, transcriptomics and proteomics, the authors can classify and annotate genes by their expression profiles and can even detect evidence of posttranscriptional gene silencing in the murine malaria species...
- Detection of genome-wide polymorphisms in the AT-rich Plasmodium falciparum genome using a high-density microarrayHongying Jiang
Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
BMC Genomics 9:398. 2008..Here we evaluate polymorphism detection accuracy of a high-density 'tiling' microarray with 2.56 million probes by comparing single feature polymorphisms (SFP) calls from the microarray with known SNP among parasite isolates...
- Utilization of genomic sequence information to develop malaria vaccinesD L Doolan
Malaria Program, Naval Medical Research Center, Silver Spring, MD 20910 7500, USA
J Exp Biol 206:3789-802. 2003..Here, we present our strategy to exploit the genomic sequence of P. falciparum for malaria vaccine development...
- Functional genomics of Plasmodium falciparum through transposon-mediated mutagenesisBharath Balu
Center for Global Health and Infectious Diseases, Department of Biological Sciences, University of Notre Dame, Notre Dame, IN 46556, USA
Cell Microbiol 8:1529-36. 2006..This technique could hence be employed to set up several biological screens in this lethal protozoan parasite that may lead to identification of novel drug targets and vaccine candidates...
- Genome Evolution, Innovation and Adaptation in the ApicomplexaJessica C Kissinger; Fiscal Year: 2010..The results of these studies will be shared with the larger research community via publication and deposition in community databases and Bioinformatics Resource Centers. ..
- Novel Anti-Malarials by Combinatorial PharmacogenomicsJoseph DeRisi; Fiscal Year: 2006..The compiled relationships from known anti-malarials, novel quinolines, and toxicology will then be used to guide and optimize additional synthesis efforts toward the ultimate goal of producing effective therapeutics. ..
- Identification of Tetrahymena DNA Rearrangement GenesDOUGLAS LEE CHALKER; Fiscal Year: 2010..abstract_text> ..
- GENE DISCOVERY IN TOXOPLASMA GONDIIL Sibley; Fiscal Year: 2003..In combination with the genetic tools available in T. gondii, this will greatly accelerate investigations into the complex developmental biology of apicomplexan parasites. ..
- Microfilaments in Giardia Attachment and VirulenceHEIDI ELMENDORF; Fiscal Year: 2006..This work will advance our understanding of parasite pathogenesis and develop our knowledge of microfilaments as a target for drug design. ..
- Calcium ATPases in Apicomplexan Parasites as Potential DL Sibley; Fiscal Year: 2006..Knowledge gained through these studies may improve the ability to pharmacologically inhibit key calcium regulatory enzymes and hence block motility and invasion by this important group of parasites. ..
- Mucosal Immunity to Toxoplasma gondiiL Sibley; Fiscal Year: 2007..unreadable] [unreadable] [unreadable]..
- Confocal Microscope for MicrobiologyL Sibley; Fiscal Year: 2002..This instrument will greatly strengthen the research programs of these highly productive groups by applying high-resolution confocal microscopy to the study of human pathogens...
- Transcriptional Regulation in Giardia lambliaHEIDI ELMENDORF; Fiscal Year: 2006..These studies will build an understanding of transcription in Giardia lamblia and lay foundations for study of the role of the regulation of gene expression in disease. ..
- GENETIC DISSECTION OF TOXOPLASMA GONDIIDavid Roos; Fiscal Year: 2008..In addition to their value for the analysis of folate metabolism and drug resistance, these tools should be broadly applicable to other studies on the biology and biochemistry of Toxoplasma. ..
- Adherence of malaria-infected red cellsAlan Cowman; Fiscal Year: 2009..These studies will contribute to and increased understanding of the pathophysiology of falciparum malaria. ..
- MARINER TRANSPOSONS OF MAMMALSHugh Robertson; Fiscal Year: 2001..It will also reveal how occasionally particular copies of transposons can become integral parts of their host genetic complements. ..
- Gustatory and olfactory receptors of Anopheles gambiaeHugh Robertson; Fiscal Year: 2006..This work will also contribute to our overall understanding of the molecular basis of chemoreception in insects and other animals. [unreadable] [unreadable]..
- HYPOTRICH GENOME IN DEVELOPMENT AND EVOLUTIONDavid Prescott; Fiscal Year: 2001..The experiments deal with the general problem of how an organism can manipulate its own DNA. ..
- GALACTOSE LECTIN OF E HISTOLYTICABarbara Mann; Fiscal Year: 2002..Successful completion of these experiments will provide insight into the role and identity of PTK/substrates in lectin-mediated killing and may establish the basis for novel therapeutic or prophylactic measures against amebiasis. ..
- Regulation of Growth to Differentiation TransitionsAdam Kuspa; Fiscal Year: 2008..Thus, this work should impact our ability to treat human diseases caused by defects in cellular growth control, such as cancer. ..
- TETRAHYMENA GENOME PROJECTEduardo Orias; Fiscal Year: 2008..unreadable] [unreadable] [unreadable]..
- Effects of Artemisinin on Leishmania SERCA pumpJOHN MEADE; Fiscal Year: 2008..cruzi and T. brucei SERCAs. [unreadable] [unreadable] [unreadable]..
- Plasmodium Genome DatabaseChristian Stoeckert; Fiscal Year: 2008..The overall goal of this project is to develop and maintain a database that expedites biological discovery, including the identification of drug targets and vaccine antigens useful in the fight against malaria. ..
- CRYPTOSPORIDIUM PARVUM GENOME SEQUENCING PROJECT (MINN)Mitchell Abrahamsen; Fiscal Year: 2002..parvum genome. ..
- IMPDH as a Drug Target in CryptosporidiumBoris Striepen; Fiscal Year: 2008..This work will provide urgently needed molecular reagents to understand the biology of nutrient uptake and drug resistance in this unique parasite. [unreadable] [unreadable] [unreadable]..
- Systems Biology of Plasmodium falciparum: Building and Exploring Network ModelsYufeng Wang; Fiscal Year: 2007....
- Ninth International Congress on ToxoplasmosisBoris Striepen; Fiscal Year: 2007..gondii that is not often seen in fields as high paced and[unreadable] competitive and has facilitated rapid and sustained progress in research on this pathogen[unreadable] and related parasites. ..
- Redox mechanism of cancer prevention by seleniumVADIM GLADYSHEV; Fiscal Year: 2002..These approaches should yield insight into the mechanism of the anticancer effect of selenium and may lead to the identification of human cancers most susceptible to selenium chemoprevention. ..
- MAPPING DRUG RESISTANCE GENES IN PLASMODIUM FALCIPARUMTim J Anderson; Fiscal Year: 2010..We aim to locate parasite genes underlying resistance. This will allow us to track the spread of resistance, to better understand how antimalarial drugs work, and to redesign drugs to restore their effectiveness. ..
- Identification of Transcriptional Regulatory Networks in Entamoeba HistolyticaUpinder Singh; Fiscal Year: 2008..We are interested in understanding the molecular mechanisms that the parasite uses to cause disease. [unreadable] [unreadable] [unreadable]..
- Comparative Evolutionary Genomics and Infectious DiseaseJane Carlton; Fiscal Year: 2010....
- Innate immunity and dendritic cells in cryptosporidiosisSAUL R TZIPORI; Fiscal Year: 2010..The proposed studies will provide the basis for understanding the mechanisms of innate immune recognition and response to parasite invasion necessary for future design of vaccines and other methods of interventions. ..
- Genetics of Cryptosporidium parvumGiovanni Widmer; Fiscal Year: 2010..Such valleys will be identified by quantitatively genotyping a selected and a control progeny population with a panel of single nucleotide polymorphisms. ..
- Transcription of protein-coding genes in LeishmaniaPETER JOHN MYLER; Fiscal Year: 2010..In this proposal, we will test this hypothesis and build on our current work to further characterize the chromatin-modifying enzymes that are involved in transcription initiation. ..
- Genetics of Cryptosporidium parvumGiovanni Widmer; Fiscal Year: 2008..Mutations will be mapped to the genome of C. hominis, C. muris and more distantly related apicomplexan to identify those most likely to affect protein function or transcriptional regulation. ..
- Intercellular signaling during terminal differentiationWilliam Loomis; Fiscal Year: 2009..Together these studies will add to the foundation for improvements in the diagnosis and treatment of neurological diseases. ..
- Experimental Annotation of the P. Falciparum GenomeElizabeth Winzeler; Fiscal Year: 2009..The basic genomic methods described here should serve as models for determining gene function in other parasites with difficult genetics. ..
- PROTEIN PHOSPHORYLATION IN TRYPANOSOMESMarilyn Parsons; Fiscal Year: 2010..Together these studies will signficantly expand our knowledge of the network of signaling interactions in trypanosomes, with the goal of identifying new drug and vaccine targets. ..
- Trypansoma cruzi-induced host cell signaling responsesBarbara Burleigh; Fiscal Year: 2009..cruzi infective process. It is the understanding of these basic processes that will guide our efforts toward effective prevention and control of Chagas'disease. ..
- Developing Tools to Study Encystation in Entamoeba HistolyticaUpinder Singh; Fiscal Year: 2007..We are interested in understanding the life stages of the parasite with the goal of identifying new ways to diagnose, treat, and prevent human infections. [unreadable] [unreadable] [unreadable]..
- Specific Human Monoclonal Antibodies for HUS ProphylaxisSaul Tzipori; Fiscal Year: 2007..This is not a hypothesis-driven proposal, but an essential segment for the characterization of a promising therapy for HUS, against which currently there is no effective treatment. ..
- RNA as the catalyst for screening drugs against trypanosomatidsReza Salavati; Fiscal Year: 2006..They will also contribute to development of drugs against trypanosomatids. ..
- Functional Genomics of Dictyostelium DevelopmentWilliam Loomis; Fiscal Year: 2005..Predictions derived from such networks will be tested when microarray analyses are extended to include further developmental mutants. ..
- Purine salvage in Cryptosporidium parvumMitchell Abrahamsen; Fiscal Year: 2005..In addition, these studies demonstrate our ability to use heterologous expression of C. parvum genes in yeast as a means to develop a high-throughput screening assay for the identification of specific inhibitors. ..
- SEQUENCING THE DICTYOSTELIUM GENOMEAdam Kuspa; Fiscal Year: 2004..Complete sequence data obtained from experimentally tractable organisms, such as Dictyostelium, will continue to stimulate genomic investigations in many systems, including humans. ..
- A Bioinformatic Approach to Inferring Protein ContactsMARCELLA MCCLURE; Fiscal Year: 2005..Several experimental laboratories have agreed to test the residue contact predictions resulting from these studies for Ebola, VSV and measles. ..
- Chromosome breakage and DNA detection in TetrahymenaMeng Chao Yao; Fiscal Year: 2006..abstract_text> ..
- Gene Duplication and Genome EvolutionAustin Hughes; Fiscal Year: 2004..5) Examining the role of transposable elements in duplication of genomic segments by testing for nonrandom association between these elements and putatively duplicated blocks in the yeast genome. ..
- RECOMBINANT SHIGA-TOXIN-SPECIFIC HUMAN ANTIBODIESSaul Tzipori; Fiscal Year: 2004..abstract_text> ..
- Adhesins and Invasins of Francisella TularensisBarbara Mann; Fiscal Year: 2004..Understanding the basic mechanisms of pathogenicity will lead to a greater understanding of why this organism is such an effective pathogen, and the identification of new targets for therapies. ..
- IMMUNITY TO CRYPTOSPORIDIOSIS IN SIV INFECTED MACAQUESSaul Tzipori; Fiscal Year: 2002..An understanding of this mechanism is important for the derivation of immunotherapeutic strategies that may be necessary to control chronic cryptosporidiosis in patients with AIDS. ..
- CHAGAS DISEASE: AUTOIMMUNITY STEMS FROM PARASITE INVASION OF HOST GENOMEAntonio Teixeira; Fiscal Year: 2008..unreadable] [unreadable]..
- 2006 Thiol-based Redox Regulation and Signaling Gordon Research ConferenceVADIM GLADYSHEV; Fiscal Year: 2006..A diverse mix of established and emerging leaders in the field, and of junior and senior investigators will be included in the program. [unreadable] [unreadable] [unreadable]..
- TRYPANASOMA CRUZI GENOME-SBRIKenneth Stuart; Fiscal Year: 2004..This information will aid the development on diagnostic, therapeutic, and preventative measures, as well as further our understanding of fundamental molecular phenomena. ..
- Host fibrogenic response to Trypanosoma cruzi infectionBarbara Burleigh; Fiscal Year: 2006..cruzi infection is mediated by a stable soluble parasite factor, which may have important therapeutic potential for a broad range of fibrotic disorders. [unreadable] [unreadable]..
- Assessing Genetic Diveristy in Entamoeba HistolyticaUpinder Singh; Fiscal Year: 2006..This approach will also provide a number of novel genetic targets for the development of improved epidemiological markers, diagnostic assays, and treatment strategies. ..
- Forward Genetics in Tetrahymena: Cloning by ComplementationROBERT COYNE; Fiscal Year: 2008..Numerous other basic studies with far-reaching relevance to human health would be facilitated by the availability of the proposed freely available resource. [unreadable] [unreadable] [unreadable] [unreadable]..
- Genetic Transformation of Cryptosporidium parvumGiovanni Widmer; Fiscal Year: 2005..Approach: Transformation by electroporation of sporozoites with GFP expression plasmids; detection of transformants in cell culture by fluorescent microscopy, anti-GFP antibodies, or reverse-transcriptase PCR. ..
- Biogenesis of Dense Core Secretory Granules in TetrahymenaAARON TURKEWITZ; Fiscal Year: 2009....
- MOLECULAR NETWORKS THAT COORDINATE DIFFERENTIATIONWilliam Loomis; Fiscal Year: 2003..Together these studies will help to establish a signal transduction pathway that can serve as a model for studies in other systems. ..
- EGSi, A Tool for Gene Inactivation in TrypanosomatidsReza Salavati; Fiscal Year: 2004..abstract_text> ..
- Discovery of Allelic Variation in Plasmodium falciparumElizabeth Winzeler; Fiscal Year: 2004..The exploratory work described herein should also be useful in determining whether hybridization-based methods could be used to identify particular isolates of other microbial pathogens, including those that may be used in bioterrorism. ..
- A Linkage Map for Schistosoma mansoniTim Anderson; Fiscal Year: 2008..Development of a linkage map will pave the way for finding these genes. [unreadable] [unreadable] [unreadable]..
- Comparative Analysis of Editosome in TrypanosomatidsReza Salavati; Fiscal Year: 2003..These analyses will be useful for understanding the function of the editosome and its components and also for development of drugs that could be effective for all three species. ..
- "Vivax Malaria Research: 2005 and Beyond"Jane Carlton; Fiscal Year: 2005..vivax with P. falciparum, epidemiology and diversity studies, vectors and transmission dynamics, and a bioinformatics workshop for researchers and students to learn to manipulate Plasmodium sequence data. ..
- Studies on Cryptosporidium parvum Type 1Saul Tzipori; Fiscal Year: 2006..The third aim investigates the extent of genetic variation and exchange within and between the two types of C. parvum to determine whether they belong to one or two species. ..
- TRYPANOSOMA CRUZI MUCOSAL & SYSTEMIC PROTECTIVE IMMUNITYDANIEL HOFT; Fiscal Year: 2008..Chemokine and/or integrin receptor expression will be studied by flow cytometry and RT-Real-Time PCR, and the in vivo effects of defective chemokine and integrin binding and/or signaling will be evaluated. [unreadable] [unreadable]..
- Generation of Reagents for E. bieneusi Genome SequencingSaul Tzipori; Fiscal Year: 2003..The outcome of this proposal will lead to a submission of a full proposal to sequence the EB genome. ..
- PIG MODEL FOR E BIENEUSISaul Tzipori; Fiscal Year: 2002..Given the cost and nature of the simian SIV model it is unlikely this model would serve the same function. ..