Genomes and Genes
Summary: Genes that are introduced into an organism using GENE TRANSFER TECHNIQUES.
Publications317 found, 100 shown here
- An optimized transgenesis system for Drosophila using germ-line-specific phiC31 integrasesJohannes Bischof
Frontiers in Genetics, National Center of Competence in Research, Institute of Molecular Biology, University of Zurich, Winterthurerstrasse 190, CH 8057 Zurich, Switzerland
Proc Natl Acad Sci U S A 104:3312-7. 2007..We generated a collection of lines with precisely mapped attP sites that allow the insertion of transgenes into many different predetermined intergenic locations throughout the fly genome...
- Germline transmission and tissue-specific expression of transgenes delivered by lentiviral vectorsCarlos Lois
Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA
Science 295:868-72. 2002..We have also generated transgenic rats that express GFP at high levels, suggesting that this technique can be used to produce other transgenic animal species...
- Single-copy insertion of transgenes in Caenorhabditis elegansChristian Frøkjaer-Jensen
Department of Biology and Howard Hughes Medical Institute, University of Utah, 257 South 1400 East, Salt Lake City, Utah 84112, USA
Nat Genet 40:1375-83. 2008At present, transgenes in Caenorhabditis elegans are generated by injecting DNA into the germline. The DNA assembles into a semistable extrachromosomal array composed of many copies of injected DNA...
- Induced pluripotent stem cells generated without viral integrationMatthias Stadtfeld
Massachusetts General Hospital Cancer Center and Center for Regenerative Medicine, 185 Cambridge Street, Boston, MA 02114, USA
Science 322:945-9. 2008..Adenoviral reprogramming may provide an improved method for generating and studying patient-specific stem cells and for comparing embryonic stem cells and iPS cells...
- Exploiting position effects and the gypsy retrovirus insulator to engineer precisely expressed transgenesMichele Markstein
Department of Genetics and Howard Hughes Medical Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
Nat Genet 40:476-83. 2008A major obstacle to creating precisely expressed transgenes lies in the epigenetic effects of the host chromatin that surrounds them...
- Human induced pluripotent stem cells free of vector and transgene sequencesJunying Yu
Morgridge Institute for Research, Madison, WI 53707 7365, USA
Science 324:797-801. 2009..These results demonstrate that reprogramming human somatic cells does not require genomic integration or the continued presence of exogenous reprogramming factors and removes one obstacle to the clinical application of human iPS cells...
- P[acman]: a BAC transgenic platform for targeted insertion of large DNA fragments in D. melanogasterKoen J T Venken
Program in Developmental Biology, Baylor College of Medicine, Houston, TX 77030, USA
Science 314:1747-51. 2006..This transgenesis platform should greatly facilitate structure/function analyses of most Drosophila genes...
- Mosaic analysis with a repressible cell marker for studies of gene function in neuronal morphogenesisT Lee
Department of Biological Sciences, Stanford University, California 94305, USA
Neuron 22:451-61. 1999..As an example, we show that the short stop gene is required in mushroom body neurons for the extension and guidance of their axons...
- Transgenesis by lentiviral vectors: lack of gene silencing in mammalian embryonic stem cells and preimplantation embryosAlexander Pfeifer
The Salk Institute for Biological Studies, La Jolla, CA 92037, USA
Proc Natl Acad Sci U S A 99:2140-5. 2002..Ability to transfer foreign genes into human ES cells has potential relevance for the development of gene and cell-based therapies...
- Co-expression of multiple transgenes in mouse CNS: a comparison of strategiesJ L Jankowsky
Department of Pathology, Johns Hopkins School of Medicine, 720 Rutland Ave, 558 Ross Research Building, Baltimore, MD 21205, USA
Biomol Eng 17:157-65. 2001The introduction of two transgenes into one animal is increasingly common as transgenic experiments become more sophisticated. In this study we examine two strategies for creating double transgenic founders from a single microinjection...
- piggyBac transposition reprograms fibroblasts to induced pluripotent stem cellsKnut Woltjen
Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario M5G 1X5, Canada
Nature 458:766-70. 2009..strategies involve retroviral, lentiviral, adenoviral and plasmid transfection to deliver reprogramming factor transgenes. Although the latter two methods are transient and minimize the potential for insertion mutagenesis, they are ..
- An endogenous, systemic RNAi pathway in plantsPatrice Dunoyer
Institut de Biologie Moleculaire des Plantes du CNRS, UPR2357, Universite de Strasbourg, Strasbourg Cedex, France
EMBO J 29:1699-712. 2010..Collectively, these results define the existence of a bona fide, endogenous and systemic RNAi pathway in plants that may have implications in adaptation, epiallelism and trans-generational memory...
- Role of the arabidopsis DRM methyltransferases in de novo DNA methylation and gene silencingXiaofeng Cao
Department of Molecular, Cell, and Developmental Biology, University of California, Los Angeles 90095, USA
Curr Biol 12:1138-44. 2002..Thus, drm mutants prevent the establishment but not the maintenance of gene silencing at FWA and SUP, suggesting that the DRMs encode the major de novo methylation enzymes affecting these genes...
- BAC TransgeneOmics: a high-throughput method for exploration of protein function in mammalsIna Poser
Max Planck Institute for Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, D 01307 Dresden, Germany
Nat Methods 5:409-15. 2008..The large size of the BAC transgenes ensures the presence of most, if not all, regulatory elements and results in expression that closely matches ..
- Gene therapy for Leber's congenital amaurosis is safe and effective through 1.5 years after vector administrationFrancesca Simonelli
Department of Ophthalmology, Second University of Naples, Naples, Italy
Mol Ther 18:643-50. 2010..The safety of the intervention and the stability of the improvement in visual and retinal function in these subjects support the use of AAV-mediated gene augmentation therapy for treatment of inherited retinal diseases...
- Intravascular AAV9 preferentially targets neonatal neurons and adult astrocytesKevin D Foust
Center for Gene Therapy, The Research Institute at Nationwide Children s Hospital, 700 Children s Drive, Columbus, Ohio 43205, USA
Nat Biotechnol 27:59-65. 2009..It may also be useful for rapid postnatal genetic manipulations in basic neuroscience studies...
- Control of memory formation through regulated expression of a CaMKII transgeneM Mayford
Center for Neurobiology and Behavior, College of Physicians and Surgeons of Columbia University, and Howard Hughes Medical Institute, 722 West 168 Street, New York, NY 10032, USA
Science 274:1678-83. 1996..Thus, the CaMKII signaling pathway is critical for both explicit and implicit memory storage, in a manner that is independent of its potential role in development...
- Imaging neuronal subsets in transgenic mice expressing multiple spectral variants of GFPG Feng
Department of Anatomy and Neurobiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
Neuron 28:41-51. 2000..In some lines, intense labeling of small neuronal subsets provided a Golgi-like vital stain. In double transgenic mice expressing two different XFPs, it was possible to differentially label 3 neuronal subsets in a single animal...
- A shift from reversible to irreversible X inactivation is triggered during ES cell differentiationA Wutz
Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, USA
Mol Cell 5:695-705. 2000..Upon differentiation, autosomal transgenes did not effect counting, but transgenic Xist RNA induced late replication and histone H4 hypoacetylation...
- Distinct requirements for somatic and germline expression of a generally expressed Caernorhabditis elegans geneW G Kelly
Carnegie Institution of Washington, Department of Embryology, Baltimore, Maryland 21210, USA
Genetics 146:227-38. 1997..The resulting transgenes show robust expression in both germline and soma...
- Two plant viral suppressors of silencing require the ethylene-inducible host transcription factor RAV2 to block RNA silencingMatthew W Endres
Department of Biological Sciences, University of South Carolina, Columbia, South Carolina, USA
PLoS Pathog 6:e1000729. 2010....
- Suberoylanilide hydroxamic acid, a histone deacetylase inhibitor, ameliorates motor deficits in a mouse model of Huntington's diseaseEmma Hockly
Medical and Molecular Genetics, Guy s, King s and St Thomas School of Medicine, King s College London, Eighth Floor Guy s Tower, Guy s Hospital, London SE1 9RT, United Kingdom
Proc Natl Acad Sci U S A 100:2041-6. 2003..SAHA dramatically improved the motor impairment in R6/2 mice, clearly validating the pursuit of this class of compounds as HD therapeutics...
- Conditional control of gene expression in the mouseM Lewandoski
Section of Genetics of Vertebrate Development, Laboratory of Cancer and Developmental Biology, National Cancer Institute Frederick Cancer Research and Development Center, Frederick, Maryland 21702 1201, USA
Nat Rev Genet 2:743-55. 2001..Here, I review these systems and discuss certain studies that exemplify the advantages and limitations of each system...
- The complete family of genes encoding G proteins of Caenorhabditis elegansG Jansen
Division of Molecular Biology, The Netherlands Cancer Institute, Centre for Biomedical Genetics, Amsterdam
Nat Genet 21:414-9. 1999....
- Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous systemJean Livet
Department of Molecular and Cellular Biology and Center for Brain Science, Harvard University, Cambridge, Massachusetts 02138, USA
Nature 450:56-62. 2007..In Brainbow transgenes, Cre/lox recombination is used to create a stochastic choice of expression between three or more fluorescent ..
- Myc-induced T cell leukemia in transgenic zebrafishDavid M Langenau
Department of Pediatric Oncology, Dana Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA
Science 299:887-90. 2003..This transgenic model provides a platform for drug screens and for genetic screens aimed at identifying mutations that suppress or enhance c-myc- induced carcinogenesis...
- mpeg1 promoter transgenes direct macrophage-lineage expression in zebrafishFelix Ellett
Cancer and Haematology Division, Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia
Blood 117:e49-56. 2011..remove this roadblock, a macrophage-specific marker was identified (mpeg1) and its promoter used in mpeg1-driven transgenes. mpeg1-driven transgenes are expressed in macrophage-lineage cells that do not express neutrophil-marking ..
- Functional genomics in Trypanosoma brucei: a collection of vectors for the expression of tagged proteins from endogenous and ectopic gene lociSteven Kelly
Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK
Mol Biochem Parasitol 154:103-9. 2007
- Endogenous microRNA can be broadly exploited to regulate transgene expression according to tissue, lineage and differentiation stateBrian D Brown
San Raffaele Telethon Institute for Gene Therapy, San Raffaele Scientific Institute, Via Olgettina, 58, 20132 Milan, Italy
Nat Biotechnol 25:1457-67. 2007..Notably, the vectors did not detectably perturb endogenous miRNA expression or regulation of natural targets. The properties of miRNA-regulated vectors should allow for safer and more effective therapeutic applications...
- Successful attenuation of humoral immunity to viral capsid and transgenic protein following AAV-mediated gene transfer with a non-depleting CD4 antibody and cyclosporineJ H McIntosh
Department of Haematology, UCL Cancer Institute, University College London, Oxford, UK
Gene Ther 19:78-85. 2012..These observations could significantly improve the prospects of using rAAV vectors for chronic disorders by allowing for repeated vector administration and avoiding the development of antibodies to the transgene product...
- Excision of reprogramming transgenes improves the differentiation potential of iPS cells generated with a single excisable vectorCesar A Sommer
Section of Gastroenterology, Department of Medicine, Boston University School of Medicine, Boston, Massachusetts 02118, USA
Stem Cells 28:64-74. 2010The residual presence of integrated transgenes following the derivation of induced pluripotent stem (iPS) cells is highly undesirable...
- High-efficiency transduction of the mouse retina by tyrosine-mutant AAV serotype vectorsHilda Petrs-Silva
Department of Ophthalmology, University of Florida, Gainesville, Florida 32610 0284, USA
Mol Ther 17:463-71. 2009..These enhanced AAV vectors have a great potential for future therapeutic applications for retinal degenerations and ocular neovascular diseases...
- A mouse model for spinal muscular atrophyH M Hsieh-Li
Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan
Nat Genet 24:66-70. 2000..Our results demonstrate that SMN2 can partially compensate for lack of SMN1. The variable phenotypes of Smn-/-SMN2 mice reflect those seen in SMA patients, providing a mouse model for this disease...
- Regulation of heterochromatic silencing and histone H3 lysine-9 methylation by RNAiThomas A Volpe
Cold Spring Harbor Laboratory, Watson School of Biological Sciences, Cold Spring Harbor, NY 11724, USA
Science 297:1833-7. 2002..This is accompanied by transcriptional de-repression of transgenes integrated at the centromere, loss of histone H3 lysine-9 methylation, and impairment of centromere function...
- Controlling transgene expression in subcutaneous implants using a skin lotion containing the apple metabolite phloretinMarc Gitzinger
Department of Biosystems Science and Engineering, Eidgenossische Technische Hochschule Zurich, Mattenstrasse 26, CH 4058 Basel, Switzerland
Proc Natl Acad Sci U S A 106:10638-43. 2009Adjustable control of therapeutic transgenes in engineered cell implants after transdermal and topical delivery of nontoxic trigger molecules would increase convenience, patient compliance, and elimination of hepatic first-pass effect in ..
- The arterial pole of the mouse heart forms from Fgf10-expressing cells in pharyngeal mesodermR G Kelly
Departement de Biologie Moleculaire, Institut Pasteur, Paris, France
Dev Cell 1:435-40. 2001..25 and 10.5. These data suggest that arterial pole myocardium originates outside the classical heart field...
- The role of heterologous chloroplast sequence elements in transgene integration and expressionTracey Ruhlman
Department of Molecular Biology and Microbiology, College of Medicine, University of Central Florida, Orlando, Florida 32816 2364, USA
Plant Physiol 152:2088-104. 2010....
- Gene editing in human stem cells using zinc finger nucleases and integrase-defective lentiviral vector deliveryAngelo Lombardo
San Raffaele Telethon Institute for Gene Therapy, Via Olgettina, 58, 20132 Milan, Italy
Nat Biotechnol 25:1298-306. 2007..required ZFN cleavage and homologous template DNA, thus establishing a platform that can target the insertion of transgenes into a predetermined genomic site...
- A gene expression atlas of the central nervous system based on bacterial artificial chromosomesShiaoching Gong
GENSAT Project, Howard Hughes Medical Institute, The Rockefeller University, 1230 York Avenue, Box 260, New York 10021, USA
Nature 425:917-25. 2003..The atlas, library of BAC vectors and BAC transgenic mice generated in this screen provide a rich resource that allows a broad array of investigations not previously available to the neuroscience community...
- Comparative analysis of transposable element vector systems in human cellsIvana Grabundzija
Max Delbruck Center for Molecular Medicine, Berlin, Germany
Mol Ther 18:1200-9. 2010..SB, Tol2, and PB constitute complementary research tools for gene transfer in mammalian cells with important implications for fundamental and translational research...
- Complete rescue of obesity, diabetes, and infertility in db/db mice by neuron-specific LEPR-B transgenesCarl De Luca
Department of Medicine, Division of Preventative Medicine, Columbia University, New York, New York, USA
J Clin Invest 115:3484-93. 2005..We have also generated mice that are compound hemizygotes for the transgenes SYN-LEPR-B and neuron-specific enolase-LEPR B (NSE-LEPR-B)...
- Spatiotemporal rescue of memory dysfunction in DrosophilaSean E McGuire
Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX 77030, USA
Science 302:1765-8. 2003..The TARGET system offers general utility in simultaneously addressing issues of when and where gene products are required...
- Adeno-associated virus serotype 6 capsid tyrosine-to-phenylalanine mutations improve gene transfer to skeletal muscleChunping Qiao
Division of Molecular Pharmaceutics, University of North Carolina Eshelman School of Pharmacy, Chapel Hill, NC 27599, USA
Hum Gene Ther 21:1343-8. 2010..Our results suggest that tyrosine-mutant AAV6 vectors may represent powerful tools for testing muscle gene therapy in animal models and potentially in humans...
- Building a zoo of mice for genetic analyses: a comprehensive protocol for the rapid generation of BAC transgenic miceTorbjörn Johansson
Institute of Pharmacology and Toxicology, University of Zurich, Zurich, Switzerland
Genesis 48:264-80. 2010..With the provided "toolbox" of already available transgene constructs, the generation of the BAC transgenes is made easy and rapid...
- Expression of the native cholera toxin B subunit gene and assembly as functional oligomers in transgenic tobacco chloroplastsH Daniell
Department of Molecular Biology and Microbiology and Center for Discovery of Drugs and Diagnostics, University of Central Florida, 12722 Research Parkway, Orlando, FL 32826 3227, USA
J Mol Biol 311:1001-9. 2001....
- Intraneuronal Abeta, non-amyloid aggregates and neurodegeneration in a Drosophila model of Alzheimer's diseaseD C Crowther
Department of Medicine, University of Cambridge, Cambridge Institute for Medical Research, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 2XY, UK
Neuroscience 132:123-35. 2005..Moreover it provides a platform to dissect the pathways of neurodegeneration in Alzheimer's disease and to develop novel therapeutic interventions...
- Refinement of tools for targeted gene expression in DrosophilaBarret D Pfeiffer
Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA
Genetics 186:735-55. 2010..The increased strength and reliability of these optimized reagents overcome many of the previous limitations of these methods and will facilitate genetic manipulations of greater complexity and sophistication...
- Virus-free induction of pluripotency and subsequent excision of reprogramming factorsKeisuke Kaji
MRC Centre for Regenerative Medicine, Institute for Stem Cell Research, University of Edinburgh, Edinburgh EH9 3JQ, UK
Nature 458:771-5. 2009....
- Recombineering BAC transgenes for protein taggingGiovanni Ciotta
Genomics, BioInnovationsZentrum, Technische Universitaet Dresden, Am Tatzberg 47, 01307 Dresden, Germany
Methods 53:113-9. 2011..BAC (bacterial artificial chromosome) transgenes present a way to express a chosen protein at physiological levels with all regulatory elements in their native ..
- Pig transgenesis by Sleeping Beauty DNA transpositionJannik E Jakobsen
Department of Human Genetics, University of Aarhus, Wilh Meyers Allé 1240, 8000, Aarhus C, Denmark
Transgenic Res 20:533-45. 2011....
- Efficient integration of transgenes into a defined locus in human embryonic stem cellsKenji Sakurai
Institute for Frontier Medical Sciences, Kyoto University, Sakyo ku, Kyoto 606 8507, Japan
Nucleic Acids Res 38:e96. 2010..We show that reporter gene expression was responsive to doxycycline and that the resulting transgenic human ES cells retain their self-renewal capacity and pluripotency...
- Site-specific integration and expression of an anti-malarial gene in transgenic Anopheles gambiae significantly reduces Plasmodium infectionsJanet M Meredith
Centre for Applied Entomology and Parasitology, Keele University, Keele, United Kingdom
PLoS ONE 6:e14587. 2011..In the future, this technology will allow effective comparisons and informed choices to be made, potentially leading to complete transmission blockade...
- The TLR9-MyD88 pathway is critical for adaptive immune responses to adeno-associated virus gene therapy vectors in miceJiangao Zhu
Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710, USA
J Clin Invest 119:2388-98. 2009....
- The RING finger/B-box factor TAM-1 and a retinoblastoma-like protein LIN-35 modulate context-dependent gene silencing in Caenorhabditis elegansJ Hsieh
Carnegie Institution of Washington, Department of Embryology, Baltimore, Maryland 21210, USA
Genes Dev 13:2958-70. 1999..We have used tandem array transgenes as a model substrate in a screen for Caenorhabditis elegans mutants that affect context-dependent gene silencing ..
- Temporally regulated and tissue-specific gene manipulations in the adult and embryonic heart using a tamoxifen-inducible Cre proteinD S Sohal
Department of Pediatrics, University of Cincinnati, Children s Hospital Medical Center, Division of Molecular Cardiovascular Biology, Cincinnati, Ohio, USA
Circ Res 89:20-5. 2001....
- RNA polymerase IV directs silencing of endogenous DNAA J Herr
Sainsbury Laboratory, John Innes Centre, Norwich NR4 7UH, UK
Science 308:118-20. 2005..The existence of this distinct silencing polymerase may explain the paradoxical involvement of an RNA silencing pathway in maintenance of transcriptional silencing...
- Transcriptional regulation of nanog by OCT4 and SOX2David J Rodda
Stem Cell and Developmental Biology and Gene Regulation Laboratory, Genome Institute of Singapore, Singapore 138672
J Biol Chem 280:24731-7. 2005..These studies extend the understanding of the pluripotent genetic regulatory network within which the Sox2-Oct4 complex are at the top of the regulatory hierarchy...
- Activation of the NF-kappaB pathway by adeno-associated virus (AAV) vectors and its implications in immune response and gene therapyGiridhara R Jayandharan
Division of Cellular and Molecular Therapy, Department of Pediatrics, Department of Pharmacology and Therapeutics, University of Florida College of Medicine, Gainesville, FL 32611, USA
Proc Natl Acad Sci U S A 108:3743-8. 2011....
- Translating Sleeping Beauty transposition into cellular therapies: victories and challengesZsuzsanna Izsvák
Max Delbrück Center for Molecular Medicine MDC, Berlin, Germany
Bioessays 32:756-67. 2010Recent results confirm that long-term expression of therapeutic transgenes can be achieved by using a transposon-based system in primary stem cells and in vivo...
- The vasa regulatory region mediates germline expression and maternal transmission of proteins in the malaria mosquito Anopheles gambiae: a versatile tool for genetic control strategiesPhilippos A Papathanos
Imperial College London, Division of Cell and Molecular Biology, Imperial College Road, London, UK
BMC Mol Biol 10:65. 2009..Germline specific promoters are an essential component of potential vector control strategies which function by genetic drive, however suitable promoters are not currently available for the main human malaria vector Anopheles gambiae...
- Notch and Kras reprogram pancreatic acinar cells to ductal intraepithelial neoplasiaJean Paul De La O
Department of Human Genetics, University of Utah, 15 North 2030 East, Room 2100, Salt Lake City, UT 84112, USA
Proc Natl Acad Sci U S A 105:18907-12. 2008..At the cellular level, Notch/Kras coactivation promotes rapid reprogramming of acinar cells to a duct-like phenotype, providing an explanation for how a characteristically ductal tumor can arise from nonductal acinar cells...
- Transient and stable transgene expression in human embryonic stem cellsChee Gee Liew
Centre for Stem Cell Biology, Department of Biomedical Science, Western Bank, University of Sheffield, Sheffield S10 2TN, United Kingdom
Stem Cells 25:1521-8. 2007..Our data also demonstrate that pUbiC, pR26, pCAGG, and pCAG are more superior to the pCMV for generation of stable transfectants in hES cells. Disclosure of potential conflicts of interest is found at the end of this article...
- Dual roles for glucokinase in glucose homeostasis as determined by liver and pancreatic beta cell-specific gene knock-outs using Cre recombinaseC Postic
Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
J Biol Chem 274:305-15. 1999..beta cell- and hepatocyte-specific expression of Cre was achieved using transgenes that contain either insulin or albumin promoter/enhancer sequences...
- High-level expression of a suite of thermostable cell wall-degrading enzymes from the chloroplast genomeKerstin Petersen
Max Planck Institut fur Molekulare Pflanzenphysiologie, Am Muhlenberg 1, 14476 Potsdam Golm, Germany
Plant Mol Biol 76:311-21. 2011..Although further optimization is needed, our data indicate that transgenic plastids offer great potential for the production of enzyme cocktails for the bioconversion of cellulosic biomass...
- A transposon-mediated gene trap approach identifies developmentally regulated genes in zebrafishKoichi Kawakami
Division of Molecular and Developmental Biology, National Institute of Genetics, 1111 Yata, Mishima, Shizuoka 411 8540, Japan
Dev Cell 7:133-44. 2004..We propose our gene trap approach should facilitate studies of vertebrate development and organogenesis...
- High-level sustained transgene expression in human embryonic stem cells using lentiviral vectorsYue Ma
National Primate Research Center, School of Medicine, University of Wisconsin, Madison, Wisconsin, USA
Stem Cells 21:111-7. 2003Here we describe the sustained expression of transgenes introduced into human embryonic stem (ES) cells using self-inactivating lentiviral vectors...
- Stable integration and conditional expression of electroporated transgenes in chicken embryosYuki Sato
Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916 5, Takayama, Ikoma, Nara, 630 0192, Japan
Dev Biol 305:616-24. 2007..However, the conventional electroporation technique fails to retain the expression of transgenes for more than several days because transgenes are not integrated into the genome...
- Gene therapy for red-green colour blindness in adult primatesKatherine Mancuso
Department of Ophthalmology, Box 356485, University of Washington, 1959 North East Pacific Street, Seattle, Washington 98195, USA
Nature 461:784-7. 2009..Thus, trichromacy can arise from a single addition of a third cone class and it does not require an early developmental process. This provides a positive outlook for the potential of gene therapy to cure adult vision disorders...
- Only half the transcriptomic differences between resistant genetically modified and conventional rice are associated with the transgeneMaria Montero
Institut de Tecnologia Agroalimentària INTEA, Universitat de Girona, Campus Montilivi, Girona, Spain
Plant Biotechnol J 9:693-702. 2011..Unintended effects might, as such, putatively result in widening the self-resistance characteristics because of the transgene in GM plants...
- PiggyBac transposon-mediated, reversible gene transfer in human embryonic stem cellsYou Tzung Chen
Graduate Institute of Clinical Genomics and Graduate Institute of Clinical Medicine, Stem Cell Core Laboratory, NTU Research Center for Medical Excellence, Taipei, Taiwan
Stem Cells Dev 19:763-71. 2010..We also demonstrate that the integrated piggyBac transposon can be removed and an undisrupted insertion site can be restored, which implies potential applications for its use in gene therapy and genetics studies...
- Real-time quantitative PCR-based system for determining transgene copy number in transgenic animalsMaria Ballester
Departament de Ciencia Animal i dels Aliments, Facultat de Veterinaria, Universitat Autonoma de Barcelona, Bellaterra, Spain
Biotechniques 37:610-3. 2004..The assay's discrimination ability from our results is of at least six copies and, similar to the limitations of the blotting techniques, the accuracy of the quantification diminishes when the transgene copy number is high...
- Transgene excision in pollen using a codon optimized serine resolvase CinH-RS2 site-specific recombination systemHong S Moon
Department of Plant Sciences, University of Tennessee, Knoxville, TN 37996, USA
Plant Mol Biol 75:621-31. 2011..a major environmental and regulatory concern in transgenic crop cultivation, could be alleviated by removing transgenes from pollen, the most frequent vector for transgene flow...
- In vivo trans-specific gene silencing in fungal cells by in planta expression of a double-stranded RNAMaria Laine P Tinoco
EMBRAPA Recursos Geneticos e Biotecnologia, PqEB W5 Norte, 70770 900, Brasilia, DF, Brazil
BMC Biol 8:27. 2010..Specific inhibition of gene expression by RNAi has been shown to be suitable for a multitude of phytopathogenic filamentous fungi. However, double-stranded (ds)RNA/small interfering (si)RNA silencing effect has not been observed in vivo...
- Recombinase mediated cassette exchange into genomic targets using an adenovirus vectorDavid A Sorrell
Molecular Recognition Group, Hannah Research Institute, Ayr, KA6 5HL, UK
Nucleic Acids Res 38:e123. 2010..This demonstrates that an RMCE system in the context of a viral vector allows the site directed insertion of a transgene into a defined genomic site...
- Use of the Gal4-UAS technique for targeted gene expression in the zebrafishN Scheer
Institut fur Entwicklungsbiologie, Universitat zu Koln, 50923, Cologne, Germany
Mech Dev 80:153-8. 1999..This work smoothes the way for a novel method of misexpression of gene products in zebrafish in order to analyze the function of genes in developmental processes...
- Cell culture-induced gradual and frequent epigenetic reprogramming of invertedly repeated tobacco transgene epiallelesKaterina Krizova
Institute of Biophysics, Academy of Sciences of the Czech Republic, 612 65 Brno, Czech Republic
Plant Physiol 149:1493-504. 2009Using a two-component transgene system involving two epiallelic variants of the invertedly repeated transgenes in locus 1 (Lo1) and a homologous single-copy transgene locus 2 (Lo2), we have studied the stability of the methylation ..
- Origin of the polycomb repressive complex 2 and gene silencing by an E(z) homolog in the unicellular alga ChlamydomonasScott Shaver
School of Biological Sciences and Center for Plant Science Innovation, University of Nebraska, Lincoln, NE, USA
Epigenetics 5:301-12. 2010..RNAi-mediated suppression of EZH led to defects in the silencing of transgenes and retrotransposons as well as to a global increase in histone post-translational modifications associated with ..
- Gene flow from genetically modified rice to its wild relatives: Assessing potential ecological consequencesBao Rong Lu
Ministry of Education Key Laboratory for Biodiversity and Ecological Engineering, Institute of Biodiversity Science, Fudan University, Handan Road 220, Shanghai 200433, China
Biotechnol Adv 27:1083-91. 2009..b>Transgenes may persist to and spread in wild populations after gene flow, resulting unwanted ecological consequences...
- Identification of genes required for de novo DNA methylation in ArabidopsisMaxim V C Greenberg
Department of Molecular, Cell and Developmental Biology, University of California Davis, USA
Epigenetics 6:344-54. 2011..Mutations were mapped and genes cloned by both traditional and whole genome sequencing approaches. The methodologies and the mutant alleles discovered will be instrumental in further studies of de novo DNA methylation...
- Functional and behavioral restoration of vision by gene therapy in the guanylate cyclase-1 (GC1) knockout mouseShannon E Boye
Department of Ophthalmology, College of Medicine, University of Florida, Gainesville, Florida, United States of America
PLoS ONE 5:e11306. 2010..The purpose of this study was to test whether delivery of functional GC1 to cone cells of the postnatal GC1KO mouse could restore function to these cells...
- Position effect variegation and imprinting of transgenes in lymphocytesAdam Williams
Division of Molecular Immunology, The National Institute for Medical Research, London, NW71AA, UK
Nucleic Acids Res 36:2320-9. 2008..In addition, transgenes integrated as repeated arrays are susceptible to repeat-induced gene silencing...
- Functionality of the GAL4/UAS system in Tribolium requires the use of endogenous core promotersJohannes B Schinko
Ernst Caspari Haus, Georg August University Gottingen, Justus von Liebig Weg11, 37077 Gottingen, Germany
BMC Dev Biol 10:53. 2010..The genetic toolbox for Tribolium research has expanded in the past years but spatio-temporally controlled misexpression of genes has not been possible so far...
- Safety and efficacy of regional intravenous (r.i.) versus intramuscular (i.m.) delivery of rAAV1 and rAAV8 to nonhuman primate skeletal muscleAlice Toromanoff
INSERM UMR 649, CHU Nantes, Faculte de Medecine, Universite de Nantes, Nantes, France
Mol Ther 16:1291-9. 2008..However, regardless of the mode of delivery, concerns continue to be raised by the presence of vector sequences detected at distant sites...
- Antigen processing by nardilysin and thimet oligopeptidase generates cytotoxic T cell epitopesJan H Kessler
Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Leiden, The Netherlands
Nat Immunol 12:45-53. 2011..By broadening the antigenic peptide repertoire, nardilysin and TOP strengthen the immune defense against intracellular pathogens and cancer...
- Minicircle DNA vectors devoid of bacterial DNA result in persistent and high-level transgene expression in vivoZhi Ying Chen
Department of Pediatrics and Department of Genetics, Stanford University School of Medicine, Stanford, California 94305, USA
Mol Ther 8:495-500. 2003..Our data suggest that minicircles are capable of expressing high and persistent levels of therapeutic products in vivo and have a great potential to serve as episomal vectors for the treatment of a wide variety of diseases...
- Electroporation facilitates introduction of reporter transgenes and virions into schistosome eggsKristine J Kines
Department of Microbiology, Immunology and Tropical Medicine, George Washington University Medical Center, Washington, DC, United States of America
PLoS Negl Trop Dis 4:e593. 2010The schistosome egg represents an attractive developmental stage at which to target transgenes because of the high ratio of germ to somatic cells, because the transgene might be propagated and amplified by infecting snails with the ..
- Efficient mammalian germline transgenesis by cis-enhanced Sleeping Beauty transpositionDaniel F Carlson
The Center for Genome Engineering, Minneapolis, MN, USA
Transgenic Res 20:29-45. 2011..The artificial methylation was reprogrammed in the early embryo, leading to founders that express the transgenes. We also noted differences in transgene insertion number and structure (single-insert versus concatemer) based ..
- Real-time PCR to determine transgene copy number and to quantitate the biolocalization of adoptively transferred cells from EGFP-transgenic miceMolishree Joshi
Department of Surgery, East Carolina University, Greenville, NC, USA
Biotechniques 45:247-58. 2008..This qPCR methodology offers specific, objective, and rapid quantitation, uncomplicated by tissue autofluorescence, and should be readily transferable to other in vivo models to quantitate the biolocalization of transplanted cells...
- Arabidopsis HEN1: a genetic link between endogenous miRNA controlling development and siRNA controlling transgene silencing and virus resistanceStéphanie Boutet
Laboratoire de Biologie Cellulaire, INRA, 78026 Versailles Cedex, France
Curr Biol 13:843-8. 2003..HEN1 contribute to resistance against cucumber mosaic virus, but not to siRNA and IR-PTGS triggered by hairpin transgenes directly producing perfect dsRNA; and iv) the actions of HEN1 in miRNA/development and siRNA/S-PTGS can be ..
- Coiled-coil-mediated dimerization is not required for myosin VI to stabilize actin during spermatid individualization in Drosophila melanogasterTatsuhiko Noguchi
Center for Developmental Biology, RIKEN, Kobe, Japan
Mol Biol Cell 20:358-67. 2009..We conclude that myosin VI does not need to dimerize via the predicted coiled coil to stabilize actin in vivo...
- A new DAF-16 isoform regulates longevityEun Soo Kwon
Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
Nature 466:498-502. 2010..Importantly, in mammals, four FOXO genes have overlapping and different functions, and in C. elegans, a single FOXO/DAF-16 uses distinct isoforms to fine-tune the IIS-mediated processes in the context of a whole organism...
- Novel tag-and-exchange (RMCE) strategies generate master cell clones with predictable and stable transgene expression propertiesJunhua Qiao
Department of Molecular Biotechnology Epigenetic Regulation, Helmholtz Centre for Infection Research, Braunschweig, Germany
J Mol Biol 390:579-94. 2009..Exemplified by Chinese hamster ovary cells, the strategy proves to be successful even for cell lines with an unstable genotype...
- Changes in adeno-associated virus-mediated gene delivery in retinal degenerationKathleen D Kolstad
Department of Molecular and Cellular Biology and Helen Wills Neuroscience Institute, University of California at Berkeley, Berkeley, CA 94720 3190, USA
Hum Gene Ther 21:571-8. 2010..Furthermore, these results indicate that gene delivery to the outer nuclear layer may be achieved by noninvasive intravitreal AAV administration in the diseased state...
- Lentiviral vectors containing an enhancer-less ubiquitously acting chromatin opening element (UCOE) provide highly reproducible and stable transgene expression in hematopoietic cellsFang Zhang
Centre for Immunodeficiency, Molecular Immunology Unit, Institute of Child Health, University College London, London, United Kingdom
Blood 110:1448-57. 2007..These properties are achieved in the absence of classic enhancer activity and therefore may confer a high safety profile...
- The GAL4 system : a versatile system for the expression of genesDavid A Elliott
Wellcome Trust Cancer Research UK Gurdon Institute, and Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge, UK
Methods Mol Biol 420:79-95. 2008..Here we describe the GAL4 system highlighting the properties that make it a powerful tool for the analysis of gene function in Drosophila and higher organisms...
- CyMATE: a new tool for methylation analysis of plant genomic DNA after bisulphite sequencingJennifer Hetzl
Institute for Computer Graphics and Algorithms, Vienna University of Technology, Favoritenstrasse 9 11, A 1040 Vienna, Austria
Plant J 51:526-36. 2007..CyMATE is freely available for non-commercial use at http://www.gmi.oeaw.ac.at/CyMATE...
- Transgenes delivered by lentiviral vector are suppressed in human embryonic stem cells in a promoter-dependent mannerXiaofeng Xia
WiCell Research Institute, University of Wisconsin Madison, Madison, WI 53705, USA
Stem Cells Dev 16:167-76. 2007..Cautions should be taken to choose the optimal promoter when lentiviruses are used for genetic modification of human ES cells...
- Specialized cells tag sexual and species identity in Drosophila melanogasterJean Christophe Billeter
Department of Biology, University of Toronto at Mississauga, 3359 Mississauga Road, Mississauga, Ontario L5L 1C6, Canada
Nature 461:987-91. 2009..These results identify a common mechanism for sexual and species recognition regulated by cuticular hydrocarbons...
- Gene therapy rescues cone function in congenital achromatopsiaAndras M Komaromy
Department of Clinical Studies, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
Hum Mol Genet 19:2581-93. 2010..Our results hold promise for future clinical trials of cone-directed gene therapy in achromatopsia and other cone-specific disorders...
- Extended plasma circulation time and decreased toxicity of polymer-coated adenovirusN K Green
Hybrid Systems Ltd, Oxford BioBusiness Centre, Littlemore Park, Littlemore, Oxford, UK
Gene Ther 11:1256-63. 2004..Such an increase in the bioavailability of adenovirus, coupled with substantial decreases in toxicity and unwanted transgene expression is an important step towards producing systemically available tumour-targeted viruses...
- Silencing of episomal transgene expression by plasmid bacterial DNA elements in vivoZ Y Chen
Departments of Pediatrics and Genetics, Stanford University School of Medicine, 200 Pasteur Drive, Room G305, Stanford, CA 94305 5208, USA
Gene Ther 11:856-64. 2004..Together, these results further established that the DNA silencing effect was mediated by a covalent linkage of the expression cassette and the bacteria DNA elements...
- Mutant EGF Receptor-Dependent Lung Cancer in Human Cell Lines and Transgenic MiceKATERINA ABIGAIL POLITI; Fiscal Year: 2010..of this award, we have characterized mouse models of lung adenocarcinoma initiated by doxycycline-inducible transgenes encoding the two common forms of mutant EGFR found in the corresponding type of human cancer...
- MECHANISMS OF AUTOIMMUNE ARTHRITISEDWARD ROSLONIEC; Fiscal Year: 2001..These transgenes were subsequently shown to confer susceptibility to collagen induced arthritis in normally resistant B10.M...
- Mechanisms of Posttranscriptional Gene SilencingHeriberto Cerutti; Fiscal Year: 2004..In fungi, plants, and animals the introduction of transgenes can lead to the silencing of homologous sequences by a variety of epigenetic phenomena operating at both ..
- Studies of Collagen Gene Regulation in Two Murine ModelsStephen Clark; Fiscal Year: 2005..These elements will be defined by studying the expression of Collal CAT reporter transgenes bearing various segments of the 5' promoter region as well as specific deletions of the first intron...
- MECHANISMS OF IGE SYNTHESIS BY B CELLSRaif Geha; Fiscal Year: 2006..assess the role of TRAF proteins in CD40 mediated isotype switching, we have examined the ability of mutant CD40 transgenes that lack one or both TRAF binding sites (deltaTRAF6, deltaTRAF2/3 and deltaTRAFs respectively) to reconstitute ..
- STRATEGIES FOR PRIMATE TRANSGENESISThaddeus Golos; Fiscal Year: 2003..Specific Aim 1 is to deliver transgenes to rhesus monkey preimplantation embryos with Epstein-Barr Virus (EBV)-based and retrovirus-based vectors...
- MECHANISMS OF IGE SYNTHESIS BY B CELLSRaif Geha; Fiscal Year: 2007..assess the role of TRAF proteins in CD40 mediated isotype switching, we have examined the ability of mutant CD40 transgenes that lack one or both TRAF binding sites (ATRAF6, ATRAF2/3 and ATRAFs respectively) to reconstitute isotype ..
- Selection & Regulation of B Lymphocytes in IDDMJames Thomas; Fiscal Year: 2006..To better understand the role of B lymphocytes in IDDM, NOD mice were produced that express immunoglobulin transgenes (Tg) from anti-insulin mab125...
- IN VIVO NOD EVALUATION OF A PATHOGENIC INSULIN PEPTIDEGeorge Eisenbarth; Fiscal Year: 2005..Our long term goal is to combine the insulin 1 and 2 knockouts with transgenes encoding altered insulin B:9-23 sequences in the same NOD strain and evaluate whether the B:9-23 native sequence ..
- Development of Novel AIDS Gene Therapy Strategies in the Macaque SHIV ModelHans Peter Kiem; Fiscal Year: 2010..The anti-HIV vectors we propose to test contain MGMT transgenes to allow in vivo selection post-transplantation...
- Development of Novel AIDS Gene Therapy Strategies in the Macaque SHIV ModelHans Peter Kiem; Fiscal Year: 2010..The anti-HIV vectors we propose to test contain MGMT transgenes to allow in vivo selection post-transplantation...
- SB Transposons for Gene TherapyPerry B Hackett; Fiscal Year: 2010..and validation of the Sleeping Beauty (SB) transposon system for its efficacy to catalyze integration of transgenes to cure mucopolysaccharidosis diseases in murine and canine model systems...
- Protein-induced human iPS cells for personalized cell therapy of PDKwang Soo Kim; Fiscal Year: 2010..However, current iPSCs suffer from major drawbacks including multiple viral integrations and remaining transgenes at various chromosomal locations, any of which may cause unpredictable genetic dysfunction and/or tumor ..
- Differential regulation of multiple transgenes for treatment of eye diseaseRICHARD J contact SAMULSKI; Fiscal Year: 2010..While these systems have been proven effective, the requirements prohibit differential regulation of multiple transgenes. Additionally, the requirement of co-delivering a trans-activator adds significantly to the payload required of ..
- SEQUENCES CONTROLLING H19 GENE IMPRINTINGMarisa Bartolomei; Fiscal Year: 2002..The DMD is essential to imprint H19 transgenes as well as the endogenous H19 gene, and the linked and oppositely imprinted Igf2 gene...
- LEPTIN RECEPTOR AND THE OBESITY/DIABETES SYNDROMEStreamson Chua; Fiscal Year: 2004..For A, transgenes with cell type-specific promoter/enhancers will be used to drive LeprB expression...
- Photoreceptor Degeneration Assay-Transgenic Xenopus(RMI)Nicholas Marsh Armstrong; Fiscal Year: 2005..We have developed lines of Xenopus laevis in which transgenes both cause rod photoreceptors to degenerate in a manner that resemble human retinitis pigmentosa as well as ..
- ANTIGEN-INDUCED B LYMPHOCYTE DEVELOPMENTDouglas Fearon; Fiscal Year: 2006..For Aims 2-5, mice will be created in which transgenes can be inducibly expressed in a lineage-specific manner...
- In vitro culture and transformation of Wolbachia from mosquitoesAnn Fallon; Fiscal Year: 2007..The next challenge to implementation of transgenic mosquitoes is development of tools to drive the spread of transgenes from small numbers of laboratory- manipulated mosquitoes into field populations...
- Wolbachia as an Agent for Population Replacement in Anopheles GambiaeJason Rasgon; Fiscal Year: 2007..b>Transgenes must be actively driven into natural Anopheles populations to high frequency for transgenic strategies to be ..
- THE HORMONAL CONTROL OF FETAL GROWTHA D ERCOLE; Fiscal Year: 1993..Using a number of transgenes designed to alter IGF expression, we will study in utero actions and mechanisms of action of IGFs in cultured ..