Genomes and Genes
Summary: Genes that are introduced into an organism using GENE TRANSFER TECHNIQUES.
Publications280 found, 100 shown here
- An optimized transgenesis system for Drosophila using germ-line-specific phiC31 integrasesJohannes Bischof
Frontiers in Genetics, National Center of Competence in Research, Institute of Molecular Biology, University of Zurich, Winterthurerstrasse 190, CH 8057 Zurich, Switzerland
Proc Natl Acad Sci U S A 104:3312-7. 2007..We generated a collection of lines with precisely mapped attP sites that allow the insertion of transgenes into many different predetermined intergenic locations throughout the fly genome...
- piggyBac transposition reprograms fibroblasts to induced pluripotent stem cellsKnut Woltjen
Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario M5G 1X5, Canada
Nature 458:766-70. 2009..strategies involve retroviral, lentiviral, adenoviral and plasmid transfection to deliver reprogramming factor transgenes. Although the latter two methods are transient and minimize the potential for insertion mutagenesis, they are ..
- P[acman]: a BAC transgenic platform for targeted insertion of large DNA fragments in D. melanogasterKoen J T Venken
Program in Developmental Biology, Baylor College of Medicine, Houston, TX 77030, USA
Science 314:1747-51. 2006..This transgenesis platform should greatly facilitate structure/function analyses of most Drosophila genes...
- Mosaic analysis with a repressible cell marker for studies of gene function in neuronal morphogenesisT Lee
Department of Biological Sciences, Stanford University, California 94305, USA
Neuron 22:451-61. 1999..As an example, we show that the short stop gene is required in mushroom body neurons for the extension and guidance of their axons...
- Exploiting position effects and the gypsy retrovirus insulator to engineer precisely expressed transgenesMichele Markstein
Department of Genetics and Howard Hughes Medical Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
Nat Genet 40:476-83. 2008A major obstacle to creating precisely expressed transgenes lies in the epigenetic effects of the host chromatin that surrounds them...
- Germline transmission and tissue-specific expression of transgenes delivered by lentiviral vectorsCarlos Lois
Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA
Science 295:868-72. 2002..We have also generated transgenic rats that express GFP at high levels, suggesting that this technique can be used to produce other transgenic animal species...
- Single-copy insertion of transgenes in Caenorhabditis elegansChristian Frøkjaer-Jensen
Department of Biology and Howard Hughes Medical Institute, University of Utah, 257 South 1400 East, Salt Lake City, Utah 84112, USA
Nat Genet 40:1375-83. 2008At present, transgenes in Caenorhabditis elegans are generated by injecting DNA into the germline. The DNA assembles into a semistable extrachromosomal array composed of many copies of injected DNA...
- A shift from reversible to irreversible X inactivation is triggered during ES cell differentiationA Wutz
Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, USA
Mol Cell 5:695-705. 2000..Upon differentiation, autosomal transgenes did not effect counting, but transgenic Xist RNA induced late replication and histone H4 hypoacetylation...
- Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous systemJean Livet
Department of Molecular and Cellular Biology and Center for Brain Science, Harvard University, Cambridge, Massachusetts 02138, USA
Nature 450:56-62. 2007..In Brainbow transgenes, Cre/lox recombination is used to create a stochastic choice of expression between three or more fluorescent ..
- Two plant viral suppressors of silencing require the ethylene-inducible host transcription factor RAV2 to block RNA silencingMatthew W Endres
Department of Biological Sciences, University of South Carolina, Columbia, South Carolina, USA
PLoS Pathog 6:e1000729. 2010....
- Suberoylanilide hydroxamic acid, a histone deacetylase inhibitor, ameliorates motor deficits in a mouse model of Huntington's diseaseEmma Hockly
Medical and Molecular Genetics, Guy's, King's and St. Thomas' School of Medicine, King's College London, Eighth Floor Guy's Tower, Guy's Hospital, London SE1 9RT, United Kingdom
Proc Natl Acad Sci U S A 100:2041-6. 2003..SAHA dramatically improved the motor impairment in R6/2 mice, clearly validating the pursuit of this class of compounds as HD therapeutics...
- Human induced pluripotent stem cells free of vector and transgene sequencesJunying Yu
Morgridge Institute for Research, Madison, WI 53707 7365, USA
Science 324:797-801. 2009..These results demonstrate that reprogramming human somatic cells does not require genomic integration or the continued presence of exogenous reprogramming factors and removes one obstacle to the clinical application of human iPS cells...
- Endogenous microRNA can be broadly exploited to regulate transgene expression according to tissue, lineage and differentiation stateBrian D Brown
San Raffaele Telethon Institute for Gene Therapy, San Raffaele Scientific Institute, Via Olgettina, 58, 20132 Milan, Italy
Nat Biotechnol 25:1457-67. 2007..Notably, the vectors did not detectably perturb endogenous miRNA expression or regulation of natural targets. The properties of miRNA-regulated vectors should allow for safer and more effective therapeutic applications...
- Distinct requirements for somatic and germline expression of a generally expressed Caernorhabditis elegans geneW G Kelly
Carnegie Institution of Washington, Department of Embryology, Baltimore, Maryland 21210, USA
Genetics 146:227-38. 1997..The resulting transgenes show robust expression in both germline and soma...
- Intraneuronal Abeta, non-amyloid aggregates and neurodegeneration in a Drosophila model of Alzheimer's diseaseD C Crowther
Department of Medicine, University of Cambridge, Cambridge Institute for Medical Research, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 2XY, UK
Neuroscience 132:123-35. 2005..Moreover it provides a platform to dissect the pathways of neurodegeneration in Alzheimer's disease and to develop novel therapeutic interventions...
- The role of heterologous chloroplast sequence elements in transgene integration and expressionTracey Ruhlman
Department of Molecular Biology and Microbiology, College of Medicine, University of Central Florida, Orlando, Florida 32816 2364, USA
Plant Physiol 152:2088-104. 2010....
- Gene therapy for Leber's congenital amaurosis is safe and effective through 1.5 years after vector administrationFrancesca Simonelli
Department of Ophthalmology, Second University of Naples, Naples, Italy
Mol Ther 18:643-50. 2010..The safety of the intervention and the stability of the improvement in visual and retinal function in these subjects support the use of AAV-mediated gene augmentation therapy for treatment of inherited retinal diseases...
- Excision of reprogramming transgenes improves the differentiation potential of iPS cells generated with a single excisable vectorCesar A Sommer
Section of Gastroenterology, Department of Medicine, Boston University School of Medicine, Boston, Massachusetts 02118, USA
Stem Cells 28:64-74. 2010The residual presence of integrated transgenes following the derivation of induced pluripotent stem (iPS) cells is highly undesirable...
- Efficient integration of transgenes into a defined locus in human embryonic stem cellsKenji Sakurai
Institute for Frontier Medical Sciences, Kyoto University, Sakyo ku, Kyoto 606 8507, Japan
Nucleic Acids Res 38:e96. 2010..We show that reporter gene expression was responsive to doxycycline and that the resulting transgenic human ES cells retain their self-renewal capacity and pluripotency...
- Pig transgenesis by Sleeping Beauty DNA transpositionJannik E Jakobsen
Department of Human Genetics, University of Aarhus, Wilh Meyers Allé 1240, 8000, Aarhus C, Denmark
Transgenic Res 20:533-45. 2011....
- Site-specific integration and expression of an anti-malarial gene in transgenic Anopheles gambiae significantly reduces Plasmodium infectionsJanet M Meredith
Centre for Applied Entomology and Parasitology, Keele University, Keele, United Kingdom
PLoS ONE 6:e14587. 2011..In the future, this technology will allow effective comparisons and informed choices to be made, potentially leading to complete transmission blockade...
- Induced pluripotent stem cells generated without viral integrationMatthias Stadtfeld
Massachusetts General Hospital Cancer Center and Center for Regenerative Medicine, 185 Cambridge Street, Boston, MA 02114, USA
Science 322:945-9. 2008..Adenoviral reprogramming may provide an improved method for generating and studying patient-specific stem cells and for comparing embryonic stem cells and iPS cells...
- Transgenesis by lentiviral vectors: lack of gene silencing in mammalian embryonic stem cells and preimplantation embryosAlexander Pfeifer
The Salk Institute for Biological Studies, La Jolla, CA 92037, USA
Proc Natl Acad Sci U S A 99:2140-5. 2002..Ability to transfer foreign genes into human ES cells has potential relevance for the development of gene and cell-based therapies...
- Co-expression of multiple transgenes in mouse CNS: a comparison of strategiesJ L Jankowsky
Department of Pathology, Johns Hopkins School of Medicine, 720 Rutland Ave, 558 Ross Research Building, Baltimore, MD 21205, USA
Biomol Eng 17:157-65. 2001The introduction of two transgenes into one animal is increasingly common as transgenic experiments become more sophisticated. In this study we examine two strategies for creating double transgenic founders from a single microinjection...
- Transcriptional regulation of nanog by OCT4 and SOX2David J Rodda
Stem Cell and Developmental Biology and Gene Regulation Laboratory, Genome Institute of Singapore, Singapore 138672
J Biol Chem 280:24731-7. 2005..These studies extend the understanding of the pluripotent genetic regulatory network within which the Sox2-Oct4 complex are at the top of the regulatory hierarchy...
- The arterial pole of the mouse heart forms from Fgf10-expressing cells in pharyngeal mesodermR G Kelly
Departement de Biologie Moleculaire, Institut Pasteur, Paris, France
Dev Cell 1:435-40. 2001..25 and 10.5. These data suggest that arterial pole myocardium originates outside the classical heart field...
- High-level sustained transgene expression in human embryonic stem cells using lentiviral vectorsYue Ma
National Primate Research Center, School of Medicine, University of Wisconsin, Madison, Wisconsin, USA
Stem Cells 21:111-7. 2003Here we describe the sustained expression of transgenes introduced into human embryonic stem (ES) cells using self-inactivating lentiviral vectors...
- Stable integration and conditional expression of electroporated transgenes in chicken embryosYuki Sato
Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916 5, Takayama, Ikoma, Nara, 630 0192, Japan
Dev Biol 305:616-24. 2007..However, the conventional electroporation technique fails to retain the expression of transgenes for more than several days because transgenes are not integrated into the genome...
- mpeg1 promoter transgenes direct macrophage-lineage expression in zebrafishFelix Ellett
Cancer and Haematology Division, Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia
Blood 117:e49-56. 2011..remove this roadblock, a macrophage-specific marker was identified (mpeg1) and its promoter used in mpeg1-driven transgenes. mpeg1-driven transgenes are expressed in macrophage-lineage cells that do not express neutrophil-marking ..
- Gene therapy for red-green colour blindness in adult primatesKatherine Mancuso
Department of Ophthalmology, Box 356485, University of Washington, 1959 North East Pacific Street, Seattle, Washington 98195, USA
Nature 461:784-7. 2009..Thus, trichromacy can arise from a single addition of a third cone class and it does not require an early developmental process. This provides a positive outlook for the potential of gene therapy to cure adult vision disorders...
- Refinement of tools for targeted gene expression in DrosophilaBarret D Pfeiffer
Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA
Genetics 186:735-55. 2010..The increased strength and reliability of these optimized reagents overcome many of the previous limitations of these methods and will facilitate genetic manipulations of greater complexity and sophistication...
- Cell culture-induced gradual and frequent epigenetic reprogramming of invertedly repeated tobacco transgene epiallelesKaterina Krizova
Institute of Biophysics, Academy of Sciences of the Czech Republic, 612 65 Brno, Czech Republic
Plant Physiol 149:1493-504. 2009Using a two-component transgene system involving two epiallelic variants of the invertedly repeated transgenes in locus 1 (Lo1) and a homologous single-copy transgene locus 2 (Lo2), we have studied the stability of the methylation ..
- Real-time PCR to determine transgene copy number and to quantitate the biolocalization of adoptively transferred cells from EGFP-transgenic miceMolishree Joshi
Department of Surgery, East Carolina University, Greenville, NC, USA
Biotechniques 45:247-58. 2008..This qPCR methodology offers specific, objective, and rapid quantitation, uncomplicated by tissue autofluorescence, and should be readily transferable to other in vivo models to quantitate the biolocalization of transplanted cells...
- Minicircle DNA vectors devoid of bacterial DNA result in persistent and high-level transgene expression in vivoZhi-Ying Chen
Department of Pediatrics and Department of Genetics, Stanford University School of Medicine, Stanford, California 94305, USA
Mol Ther 8:495-500. 2003..Our data suggest that minicircles are capable of expressing high and persistent levels of therapeutic products in vivo and have a great potential to serve as episomal vectors for the treatment of a wide variety of diseases...
- Arabidopsis HEN1: a genetic link between endogenous miRNA controlling development and siRNA controlling transgene silencing and virus resistanceStéphanie Boutet
Laboratoire de Biologie Cellulaire, INRA, 78026 Versailles Cedex, France
Curr Biol 13:843-8. 2003..HEN1 contribute to resistance against cucumber mosaic virus, but not to siRNA and IR-PTGS triggered by hairpin transgenes directly producing perfect dsRNA; and iv) the actions of HEN1 in miRNA/development and siRNA/S-PTGS can be ..
- Gene flow from genetically modified rice to its wild relatives: Assessing potential ecological consequencesBao Rong Lu
Ministry of Education Key Laboratory for Biodiversity and Ecological Engineering, Institute of Biodiversity Science, Fudan University, Handan Road 220, Shanghai 200433, China
Biotechnol Adv 27:1083-91. 2009..b>Transgenes may persist to and spread in wild populations after gene flow, resulting unwanted ecological consequences...
- Efficient mammalian germline transgenesis by cis-enhanced Sleeping Beauty transpositionDaniel F Carlson
The Center for Genome Engineering, Minneapolis, MN, USA
Transgenic Res 20:29-45. 2011..The artificial methylation was reprogrammed in the early embryo, leading to founders that express the transgenes. We also noted differences in transgene insertion number and structure (single-insert versus concatemer) based ..
- Antigen processing by nardilysin and thimet oligopeptidase generates cytotoxic T cell epitopesJan H Kessler
Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Leiden, The Netherlands
Nat Immunol 12:45-53. 2011..By broadening the antigenic peptide repertoire, nardilysin and TOP strengthen the immune defense against intracellular pathogens and cancer...
- Identification of genes required for de novo DNA methylation in ArabidopsisMaxim V C Greenberg
Department of Molecular, Cell and Developmental Biology, University of California Davis, USA
Epigenetics 6:344-54. 2011..Mutations were mapped and genes cloned by both traditional and whole genome sequencing approaches. The methodologies and the mutant alleles discovered will be instrumental in further studies of de novo DNA methylation...
- Position effect variegation and imprinting of transgenes in lymphocytesAdam Williams
Division of Molecular Immunology, The National Institute for Medical Research, London, NW71AA, UK
Nucleic Acids Res 36:2320-9. 2008..In addition, transgenes integrated as repeated arrays are susceptible to repeat-induced gene silencing...
- Functional and behavioral restoration of vision by gene therapy in the guanylate cyclase-1 (GC1) knockout mouseShannon E Boye
Department of Ophthalmology, College of Medicine, University of Florida, Gainesville, Florida, United States of America
PLoS ONE 5:e11306. 2010..The purpose of this study was to test whether delivery of functional GC1 to cone cells of the postnatal GC1KO mouse could restore function to these cells...
- Functionality of the GAL4/UAS system in Tribolium requires the use of endogenous core promotersJohannes B Schinko
Ernst Caspari Haus, Georg August University Gottingen, Justus von Liebig Weg11, 37077 Gottingen, Germany
BMC Dev Biol 10:53. 2010..The genetic toolbox for Tribolium research has expanded in the past years but spatio-temporally controlled misexpression of genes has not been possible so far...
- Origin of the polycomb repressive complex 2 and gene silencing by an E(z) homolog in the unicellular alga ChlamydomonasScott Shaver
School of Biological Sciences and Center for Plant Science Innovation, University of Nebraska, Lincoln, NE, USA
Epigenetics 5:301-12. 2010..RNAi-mediated suppression of EZH led to defects in the silencing of transgenes and retrotransposons as well as to a global increase in histone post-translational modifications associated with ..
- Safety and efficacy of regional intravenous (r.i.) versus intramuscular (i.m.) delivery of rAAV1 and rAAV8 to nonhuman primate skeletal muscleAlice Toromanoff
INSERM UMR 649, CHU Nantes, Faculte de Medecine, Universite de Nantes, Nantes, France
Mol Ther 16:1291-9. 2008..However, regardless of the mode of delivery, concerns continue to be raised by the presence of vector sequences detected at distant sites...
- Electroporation facilitates introduction of reporter transgenes and virions into schistosome eggsKristine J Kines
Department of Microbiology, Immunology and Tropical Medicine, George Washington University Medical Center, Washington, DC, United States of America
PLoS Negl Trop Dis 4:e593. 2010The schistosome egg represents an attractive developmental stage at which to target transgenes because of the high ratio of germ to somatic cells, because the transgene might be propagated and amplified by infecting snails with the ..
- Recombinase mediated cassette exchange into genomic targets using an adenovirus vectorDavid A Sorrell
Molecular Recognition Group, Hannah Research Institute, Ayr, KA6 5HL, UK
Nucleic Acids Res 38:e123. 2010..This demonstrates that an RMCE system in the context of a viral vector allows the site directed insertion of a transgene into a defined genomic site...
- In vivo trans-specific gene silencing in fungal cells by in planta expression of a double-stranded RNAMaria Laine P Tinoco
EMBRAPA Recursos Geneticos e Biotecnologia, PqEB W5 Norte, 70770 900, Brasilia, DF, Brazil
BMC Biol 8:27. 2010..Specific inhibition of gene expression by RNAi has been shown to be suitable for a multitude of phytopathogenic filamentous fungi. However, double-stranded (ds)RNA/small interfering (si)RNA silencing effect has not been observed in vivo...
- Use of the Gal4-UAS technique for targeted gene expression in the zebrafishN Scheer
Institut fur Entwicklungsbiologie, Universitat zu Koln, 50923, Cologne, Germany
Mech Dev 80:153-8. 1999..This work smoothes the way for a novel method of misexpression of gene products in zebrafish in order to analyze the function of genes in developmental processes...
- Novel tag-and-exchange (RMCE) strategies generate master cell clones with predictable and stable transgene expression propertiesJunhua Qiao
Department of Molecular Biotechnology Epigenetic Regulation, Helmholtz Centre for Infection Research, Braunschweig, Germany
J Mol Biol 390:579-94. 2009..Exemplified by Chinese hamster ovary cells, the strategy proves to be successful even for cell lines with an unstable genotype...
- Coiled-coil-mediated dimerization is not required for myosin VI to stabilize actin during spermatid individualization in Drosophila melanogasterTatsuhiko Noguchi
Center for Developmental Biology, RIKEN, Kobe, Japan
Mol Biol Cell 20:358-67. 2009..We conclude that myosin VI does not need to dimerize via the predicted coiled coil to stabilize actin in vivo...
- Changes in adeno-associated virus-mediated gene delivery in retinal degenerationKathleen D Kolstad
Department of Molecular and Cellular Biology and Helen Wills Neuroscience Institute, University of California at Berkeley, Berkeley, CA 94720 3190, USA
Hum Gene Ther 21:571-8. 2010..Furthermore, these results indicate that gene delivery to the outer nuclear layer may be achieved by noninvasive intravitreal AAV administration in the diseased state...
- A new DAF-16 isoform regulates longevityEun Soo Kwon
Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
Nature 466:498-502. 2010..Importantly, in mammals, four FOXO genes have overlapping and different functions, and in C. elegans, a single FOXO/DAF-16 uses distinct isoforms to fine-tune the IIS-mediated processes in the context of a whole organism...
- Lentiviral vectors containing an enhancer-less ubiquitously acting chromatin opening element (UCOE) provide highly reproducible and stable transgene expression in hematopoietic cellsFang Zhang
Centre for Immunodeficiency, Molecular Immunology Unit, Institute of Child Health, University College London, London, United Kingdom
Blood 110:1448-57. 2007..These properties are achieved in the absence of classic enhancer activity and therefore may confer a high safety profile...
- Extended plasma circulation time and decreased toxicity of polymer-coated adenovirusN K Green
Hybrid Systems Ltd, Oxford BioBusiness Centre, Littlemore Park, Littlemore, Oxford, UK
Gene Ther 11:1256-63. 2004..Such an increase in the bioavailability of adenovirus, coupled with substantial decreases in toxicity and unwanted transgene expression is an important step towards producing systemically available tumour-targeted viruses...
- Transgenes delivered by lentiviral vector are suppressed in human embryonic stem cells in a promoter-dependent mannerXiaofeng Xia
WiCell Research Institute, University of Wisconsin Madison, Madison, WI 53705, USA
Stem Cells Dev 16:167-76. 2007..Cautions should be taken to choose the optimal promoter when lentiviruses are used for genetic modification of human ES cells...
- The GAL4 system : a versatile system for the expression of genesDavid A Elliott
Wellcome Trust Cancer Research UK Gurdon Institute, and Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge, UK
Methods Mol Biol 420:79-95. 2008..Here we describe the GAL4 system highlighting the properties that make it a powerful tool for the analysis of gene function in Drosophila and higher organisms...
- Silencing of episomal transgene expression by plasmid bacterial DNA elements in vivoZ Y Chen
Departments of Pediatrics and Genetics, Stanford University School of Medicine, 200 Pasteur Drive, Room G305, Stanford, CA 94305-5208, USA
Gene Ther 11:856-64. 2004..Together, these results further established that the DNA silencing effect was mediated by a covalent linkage of the expression cassette and the bacteria DNA elements...
- Specialized cells tag sexual and species identity in Drosophila melanogasterJean Christophe Billeter
Department of Biology, University of Toronto at Mississauga, 3359 Mississauga Road, Mississauga, Ontario L5L 1C6, Canada
Nature 461:987-91. 2009..These results identify a common mechanism for sexual and species recognition regulated by cuticular hydrocarbons...
- Gene therapy rescues cone function in congenital achromatopsiaAndras M Komaromy
Department of Clinical Studies, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
Hum Mol Genet 19:2581-93. 2010..Our results hold promise for future clinical trials of cone-directed gene therapy in achromatopsia and other cone-specific disorders...
- CyMATE: a new tool for methylation analysis of plant genomic DNA after bisulphite sequencingJennifer Hetzl
Institute for Computer Graphics and Algorithms, Vienna University of Technology, Favoritenstrasse 9 11, A 1040 Vienna, Austria
Plant J 51:526-36. 2007..CyMATE is freely available for non-commercial use at http://www.gmi.oeaw.ac.at/CyMATE...
- Fast, easy and efficient: site-specific insertion of transgenes into enterobacterial chromosomes using Tn7 without need for selection of the insertion eventGregory J McKenzie
Howard Hughes Medical Institute, Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
BMC Microbiol 6:39. 2006Inserting transgenes into bacterial chromosomes is generally quite involved, requiring a selection for cells carrying the insertion, usually for drug-resistance, or multiple cumbersome manipulations, or both...
- On the role of RNA amplification in dsRNA-triggered gene silencingT Sijen
Hubrecht Laboratory, Center for Biomedical Genetics, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands
Cell 107:465-76. 2001..This amplification mechanism substantially augments the potency of RNAi-based surveillance, while ensuring that the RNAi machinery will focus on expressed mRNAs...
- BAC TransgeneOmics: a high-throughput method for exploration of protein function in mammalsIna Poser
Max Planck Institute for Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, D 01307 Dresden, Germany
Nat Methods 5:409-15. 2008..The large size of the BAC transgenes ensures the presence of most, if not all, regulatory elements and results in expression that closely matches ..
- The role of a retinoic acid response element in establishing the anterior neural expression border of Hoxd4 transgenesChristof Nolte
Department of Medicine, McGill University, Montreal, Quebec, Canada
Mech Dev 120:325-35. 2003..Hence, the setting of the Hox anterior expression border is not a simple function of the affinity of retinoid receptors for their cognate element...
- neu/ERBB2 cooperates with p53-172H during mammary tumorigenesis in transgenic miceB Li
Department of Pathology, Yale University School of Medicine, New Haven, Connecticut 06520 8023, USA
Mol Cell Biol 17:3155-63. 1997..Further experiments address potential mechanisms of cooperativity between the two transgenes. In these bitransgenic mice, we have recapitulated two common genetic lesions that occur in human breast cancer ..
- Prospects and implications of using chromatin insulators in gene therapy and transgenesisFelix Recillas-Targa
Departamento de Genetica Molecular, Instituto de Fisiologia Celular, Universidad Nacional Autonoma de Mexico, Mexico
Bioessays 26:796-807. 2004..Therefore, these elements may be very useful tools in gene therapy applications for ensuring high-level and stable expression of transgenes.
- The histone deacetylase inhibitor FK228 preferentially enhances adenovirus transgene expression in malignant cellsMerrill E Goldsmith
Cancer Therapeutics Branch, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland 20892, USA
Clin Cancer Res 9:5394-401. 2003....
- Production, concentration and titration of pseudotyped HIV-1-based lentiviral vectorsRobert H Kutner
Gene Therapy Program, Department of Medicine, Louisiana State University Health Sciences Center, 533 Bolivar Street, New Orleans, Louisiana 70112, USA
Nat Protoc 4:495-505. 2009..The vector production and titration protocol described here can be completed within 8 d...
- Carbonic anhydrase II-positive pancreatic cells are progenitors for both endocrine and exocrine pancreas after birthAkari Inada
Section of Islet Transplantation and Cell Biology, Joslin Diabetes Center, Harvard Medical School, One Joslin Place, Boston, MA 02215, USA
Proc Natl Acad Sci U S A 105:19915-9. 2008..This identification of a differentiated pancreatic cell type as an in vivo progenitor of all differentiated pancreatic cell types has implications for a potential expandable source for new islets for replenishment therapy for diabetes...
- Animal transgenesis: an overviewMiguel A Gama Sosa
Department of Psychiatry, Mount Sinai School of Medicine, One Gustave L Levy Place, New York, NY, 10029, USA
Brain Struct Funct 214:91-109. 2010..The most commonly used techniques for producing transgenic mice involves either the pronuclear injection of transgenes into fertilized oocytes or embryonic stem cell-mediated gene targeting...
- Site-specific recombination for the modification of transgenic strains of the Mediterranean fruit fly Ceratitis capitataMarc F Schetelig
Department of Developmental Biology, Georg August University Gottingen, GZMB, Ernst Caspari Haus, Johann Friedrich Blumenbach Institute of Zoology and Anthropology, 37077 Gottingen, Germany
Proc Natl Acad Sci U S A 106:18171-6. 2009..Here we describe an efficient method for the modification of previously inserted transgenes by the use of the site-specific integration system from phage phiC31 in a tephritid pest species, the ..
- Abrogation of disease development in plants expressing animal antiapoptotic genesM B Dickman
Department of Plant Pathology, University of Nebraska, Lincoln, NE 68583 0722, USA
Proc Natl Acad Sci U S A 98:6957-62. 2001..Plants expressing human Bcl-2 and Bcl-xl, nematode CED-9, or baculovirus Op-IAP transgenes conferred heritable resistance to several necrotrophic fungal pathogens, suggesting that disease development ..
- Spatiotemporal rescue of memory dysfunction in DrosophilaSean E McGuire
Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX 77030, USA
Science 302:1765-8. 2003..The TARGET system offers general utility in simultaneously addressing issues of when and where gene products are required...
- Xist yeast artificial chromosome transgenes function as X-inactivation centers only in multicopy arrays and not as single copiesE Heard
Unité de Génétique Moléculaire Murine, CNRS URA 1968, Institut Pasteur, F 75724 Paris Cedex 15, France
Mol Cell Biol 19:3156-66. 1999..Although YAC and cosmid Xist-containing transgenes have previously been reported to be capable of cis inactivation and counting, the transgenes were all present as ..
- Transgene-activated mesenchymal cells for articular cartilage repair: a comparison of primary bone marrow-, perichondrium/periosteum- and fat-derived cellsJung Park
Department of Oro-Maxillo-Facial Surgery, University of Erlangen-Nuernberg, Germany
J Gene Med 8:112-25. 2006..A chondrogenic stimulus, e.g. by transfer of BMP-2 cDNA, appears to be required for initiation and support of chondrogenic differentiation...
- Quantitative and mechanism-based investigation of post-nuclear delivery events between adenovirus and lipoplexSusumu Hama
Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Hokkaido 060 0812, Japan
Nucleic Acids Res 35:1533-43. 2007..Collectively, an improvement in nuclear decondensation and the diminution of the interaction between vector and mRNA is essential for the development of new generations of non-viral vectors...
- A novel human artificial chromosome vector provides effective cell lineage-specific transgene expression in human mesenchymal stem cellsXianying Ren
Department of Molecular and Cell Genetics, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Science, Tottori University, Yonago, Tottori 683-8503, Japan
Stem Cells 23:1608-16. 2005..chromosomes in host cells and should be free from potential insertional mutagenesis problems of conventional transgenes. Therefore, HACs have been proposed as alternative implements to cell-mediated gene therapy...
- Marker-free transgenic plants through genetically programmed auto-excisionDimitri Verweire
Laboratory of Plant Genetics, Institute for Molecular Biology and Biotechnology, Vrije Universiteit Brussel, B 1050 Brussels, Belgium
Plant Physiol 145:1220-31. 2007..Marker-free homozygous progeny plants were efficiently obtained, regardless of which promoter was used. In addition, simplification of complex transgene loci was observed...
- Gene delivery by lentivirus vectorsAdam S Cockrell
Gene Therapy Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
Mol Biotechnol 36:184-204. 2007..Concluding remarks address some of the successes in preclinical animals, and the recent transition of lentiviral vectors to human clinical trials as therapy for a variety of infectious and genetic diseases...
- Tetracycline-inducible expression systems: new strategies and practices in the transgenic mouse modelingYan Sun
Center of Experimental Animals, Sun Yat Sen University, Guangzhou 510080, China
Acta Biochim Biophys Sin (Shanghai) 39:235-46. 2007..In this review these newly developed strategies are discussed...
- Developmental expression patterns of Arabidopsis XTH genes reported by transgenes and GenevestigatorJaime Becnel
Biochemistry and Cell Biology, Rice University, Houston, TX 77005-1892, USA
Plant Mol Biol 61:451-67. 2006..of the distinct members of this family, GUS reporter fusion genes were constructed, and plants expressing these transgenes were characterized to reveal spatial and temporal patterns of expression...
- Expression of Gal4-dependent transgenes in cells of the mononuclear phagocyte system labeled with enhanced cyan fluorescent protein using Csf1r-Gal4VP16/UAS-ECFP double-transgenic miceDmitry A Ovchinnikov
Institute for Molecular Bioscience, Bldg 80, University of Queensland, Brisbane, Queensland 4072, Australia
J Leukoc Biol 83:430-3. 2008We generated double-transgenic mice carrying cointegrated tissue-specific Gal4 and Gal4 reporter transgenes to direct transgene overexpression in the mononuclear phagocyte system (MPS)...
- A focused and efficient genetic screening strategy in the mouse: identification of mutations that disrupt cortical developmentKonstantinos Zarbalis
Department of Neurology and the Ernest Gallo Clinic and Research Center, University of California at San Francisco, Emeryville, USA
PLoS Biol 2:E219. 2004..Our results also highlight the utility of focused screens in the mouse, in addition to the large-scale and broadly targeted screens that are being carried out at mutagenesis centers...
- The vasa regulatory region mediates germline expression and maternal transmission of proteins in the malaria mosquito Anopheles gambiae: a versatile tool for genetic control strategiesPhilippos A Papathanos
Imperial College London, Division of Cell and Molecular Biology, Imperial College Road, London, UK
BMC Mol Biol 10:65. 2009..Germline specific promoters are an essential component of potential vector control strategies which function by genetic drive, however suitable promoters are not currently available for the main human malaria vector Anopheles gambiae...
- Dispersal of transgenes through maize seed systems in MexicoGeorge A Dyer
Department of Agricultural and Resource Economics, University of California Davis, Davis, California, United States of America
PLoS ONE 4:e5734. 2009..We analyze the dispersal of maize transgenes via seeds in Mexico, the crop's cradle.
- N-Myc regulates a widespread euchromatic program in the human genome partially independent of its role as a classical transcription factorRebecca Cotterman
Department of Cell Biology, University of California Davis School of Medicine, Davis, California 95616, USA
Cancer Res 68:9654-62. 2008..These findings support a new dual model for Myc chromatin function with important implications for the role of Myc in cancer and stem cell biology, including that of induced pluripotent stem cells...
- Biological pacemaker engineered by nonviral gene transfer in a mouse model of complete atrioventricular blockJulien Piron
INSERM, UMR915, l institut du thorax, Nantes, France
Mol Ther 16:1937-43. 2008..In conclusion, nonviral gene transfer can produce a functional cardiac biological pacemaker regulated by sympathetic input, which improves life expectancy in a mouse model of CAVB...
- Improved efficiency and pace of generating induced pluripotent stem cells from human adult and fetal fibroblastsPrashant Mali
Stem Cell Program, Institute for Cell Engineering, The Johns Hopkins University School of Medicine, Broadway Research Building, Room 747, 733 North Broadway, Baltimore, Maryland 21205, USA
Stem Cells 26:1998-2005. 2008..Using this improved approach, we also generated hES-like cells from homozygous fibroblasts containing the sickle cell anemia mutation Hemoglobin Sickle. Disclosure of potential conflicts of interest is found at the end of this article...
- MAZe: a tool for mosaic analysis of gene function in zebrafishRussell T Collins
Vertebrate Development Laboratory, Cancer Research UK London Research Institute, London, UK
Nat Methods 7:219-23. 2010..any desired gene product can be generated by crossing MAZe fish with other lines containing UAS-driven transgenes. The number of clones induced, and their time of origin, could be varied by adjusting heat-shock timing and ..
- Distinct RNA-dependent RNA polymerases are required for RNAi triggered by double-stranded RNA versus truncated transgenes in Paramecium tetraureliaSimone Marker
Department of Biology, University of Kaiserslautern, Gottlieb Daimler Street, 67663 Kaiserslautern, Germany
Nucleic Acids Res 38:4092-107. 2010..In this organism, RNAi can be triggered either by high-copy, truncated transgenes or by directly feeding cells with double-stranded RNA (dsRNA)...
- An antibiotic selection marker for nematode transgenesisRosina Giordano-Santini
Genome Regulation and Evolution, INSERM U869, Institut Europeen de Chimie et Biologie, Pessac, France
Nat Methods 7:721-3. 2010..This selection system allows hands-off maintenance and enrichment of transgenic worms carrying non-integrated transgenes on selective plates...
- MicroRNA-regulated, systemically delivered rAAV9: a step closer to CNS-restricted transgene expressionJun Xie
Gene Therapy Center, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
Mol Ther 19:526-35. 2011..Our findings promise to facilitate the development of miRNA-regulated rAAV for CNS-targeted gene delivery and other applications...
- Correction of a genetic defect in multipotent germline stem cells using a human artificial chromosomeY Kazuki
Department of Biomedical Science, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Science, Tottori University, Yonago, Tottori, Japan
Gene Ther 15:617-24. 2008..Therefore, the combination of using mGS cells with HACs provides a new tool for gene and cell therapies. The next step is to demonstrate functional restoration using animal models for future gene therapy...
- Recombinant adenoviral vectors turn on the type I interferon system without inhibition of transgene expression and viral replicationEduardo Huarte
Center for Applied Medical Research, School of Medicine, and University Clinic, University of Navarra, Avenida Pio XII, 55, 31008 Pamplona, Spain
Mol Ther 14:129-38. 2006..However, type I IFN might modulate antitumor and antiadenoviral immune responses and thus influence the outcome of gene immunotherapy...
- Hair follicle stem cell-targeted gene transfer and reconstitution systemY Sugiyama-Nakagiri
Department of Dermatology, Hokkaido University Graduate School of Medicine, Sapporo 060-8638, Japan
Gene Ther 13:732-7. 2006..In addition, transgene expression was observed for at least 6 months. This HF stem cell-targeted gene transfer and reconstitution system provides reliable gene-function analysis and gene therapy...
- Noninvasive monitoring of long-term lentiviral vector-mediated gene expression in rodent brain with bioluminescence imagingChristophe M Deroose
Molecular Small Animal Imaging Center (MoSAIC) and Department of Nuclear Medicine, University Hospital Leuven, Belgium
Mol Ther 14:423-31. 2006..This is the first study to document noninvasive monitoring of long-term transgene expression in the adult mouse brain and provides the basis for applying BLI in the study of brain disease and gene therapeutic strategies...
- Evaluation of adenovirus vectors containing serotype 35 fibers for vaccinationNelson DiPaolo
Division of Medical Genetics, Department of Medicine, University of Washington, Seattle, WA 98195, USA
Mol Ther 13:756-65. 2006..Overall, compared to Ad5 vectors, Ad5/35 vectors had a better safety profile, reflected by lower serum levels of proinflammatory cytokines...
- Increased glia-specific transgene expression with glial fibrillary acidic protein promoters containing multiple enhancer elementsBertie de Leeuw
Department of Neurology, Erasmus University Medical Center, Rotterdam, The Netherlands
J Neurosci Res 83:744-53. 2006..Adenoviral vectors containing these enhanced promoters may be useful in glioma gene therapy...
- RISC control for gene therapyWaseem Qasim
Nat Biotechnol 24:661-2. 2006
- Long-term persistence of gene expression from adeno-associated virus serotype 5 in the mouse airwaysS G Sumner-Jones
Nuffield Department of Clinical Laboratory Sciences, John Radcliffe Hospital, University of Oxford, Oxford, UK
Gene Ther 13:1703-13. 2006Recombinant adeno-associated virus vectors based on serotype 2 (rAAV2) have been used to deliver transgenes to the airways in a variety of pre-clinical and clinical studies...
- Gene transfer and genetic modification of embryonic stem cells by Cre- and Cre-PR-expressing MESV-based retroviral vectorsStelios Psarras
INSERM U373, Institut Necker, Paris, France
J Gene Med 6:32-42. 2004..CONCLUSIONS: The MESV-based retroviral systems, when combined with hormone inducible Cre, represent efficient tools for the transfer of Cre activity in ES cells...
- Mutant EGF Receptor-Dependent Lung Cancer in Human Cell Lines and Transgenic MiceKATERINA ABIGAIL POLITI; Fiscal Year: 2010..of this award, we have characterized mouse models of lung adenocarcinoma initiated by doxycycline-inducible transgenes encoding the two common forms of mutant EGFR found in the corresponding type of human cancer...
- MECHANISMS OF AUTOIMMUNE ARTHRITISEDWARD ROSLONIEC; Fiscal Year: 2001..These transgenes were subsequently shown to confer susceptibility to collagen induced arthritis in normally resistant B10.M...
- Mechanisms of Posttranscriptional Gene SilencingHeriberto Cerutti; Fiscal Year: 2004..In fungi, plants, and animals the introduction of transgenes can lead to the silencing of homologous sequences by a variety of epigenetic phenomena operating at both ..
- Studies of Collagen Gene Regulation in Two Murine ModelsStephen Clark; Fiscal Year: 2005..These elements will be defined by studying the expression of Collal CAT reporter transgenes bearing various segments of the 5' promoter region as well as specific deletions of the first intron...
- STRATEGIES FOR PRIMATE TRANSGENESISThaddeus Golos; Fiscal Year: 2003..Specific Aim 1 is to deliver transgenes to rhesus monkey preimplantation embryos with Epstein-Barr Virus (EBV)-based and retrovirus-based vectors...
- MECHANISMS OF IGE SYNTHESIS BY B CELLSRaif Geha; Fiscal Year: 2007..assess the role of TRAF proteins in CD40 mediated isotype switching, we have examined the ability of mutant CD40 transgenes that lack one or both TRAF binding sites (ATRAF6, ATRAF2/3 and ATRAFs respectively) to reconstitute isotype ..
- MECHANISMS OF IGE SYNTHESIS BY B CELLSRaif Geha; Fiscal Year: 2006..assess the role of TRAF proteins in CD40 mediated isotype switching, we have examined the ability of mutant CD40 transgenes that lack one or both TRAF binding sites (deltaTRAF6, deltaTRAF2/3 and deltaTRAFs respectively) to reconstitute ..
- Selection & Regulation of B Lymphocytes in IDDMJames Thomas; Fiscal Year: 2006..To better understand the role of B lymphocytes in IDDM, NOD mice were produced that express immunoglobulin transgenes (Tg) from anti-insulin mab125...
- IN VIVO NOD EVALUATION OF A PATHOGENIC INSULIN PEPTIDEGeorge Eisenbarth; Fiscal Year: 2005..Our long term goal is to combine the insulin 1 and 2 knockouts with transgenes encoding altered insulin B:9-23 sequences in the same NOD strain and evaluate whether the B:9-23 native sequence ..
- Development of Novel AIDS Gene Therapy Strategies in the Macaque SHIV ModelHans Peter Kiem; Fiscal Year: 2010..The anti-HIV vectors we propose to test contain MGMT transgenes to allow in vivo selection post-transplantation...
- Development of Novel AIDS Gene Therapy Strategies in the Macaque SHIV ModelHans Peter Kiem; Fiscal Year: 2010..The anti-HIV vectors we propose to test contain MGMT transgenes to allow in vivo selection post-transplantation...
- SB Transposons for Gene TherapyPerry B Hackett; Fiscal Year: 2010..and validation of the Sleeping Beauty (SB) transposon system for its efficacy to catalyze integration of transgenes to cure mucopolysaccharidosis diseases in murine and canine model systems...
- Protein-induced human iPS cells for personalized cell therapy of PDKwang Soo Kim; Fiscal Year: 2010..However, current iPSCs suffer from major drawbacks including multiple viral integrations and remaining transgenes at various chromosomal locations, any of which may cause unpredictable genetic dysfunction and/or tumor ..
- Differential regulation of multiple transgenes for treatment of eye diseaseRICHARD J contact SAMULSKI; Fiscal Year: 2010..While these systems have been proven effective, the requirements prohibit differential regulation of multiple transgenes. Additionally, the requirement of co-delivering a trans-activator adds significantly to the payload required of ..
- SEQUENCES CONTROLLING H19 GENE IMPRINTINGMarisa Bartolomei; Fiscal Year: 2002..The DMD is essential to imprint H19 transgenes as well as the endogenous H19 gene, and the linked and oppositely imprinted Igf2 gene...
- Photoreceptor Degeneration Assay-Transgenic Xenopus(RMI)Nicholas Marsh Armstrong; Fiscal Year: 2005..We have developed lines of Xenopus laevis in which transgenes both cause rod photoreceptors to degenerate in a manner that resemble human retinitis pigmentosa as well as ..
- LEPTIN RECEPTOR AND THE OBESITY/DIABETES SYNDROMEStreamson Chua; Fiscal Year: 2004..For A, transgenes with cell type-specific promoter/enhancers will be used to drive LeprB expression...
- ANTIGEN-INDUCED B LYMPHOCYTE DEVELOPMENTDouglas Fearon; Fiscal Year: 2006..For Aims 2-5, mice will be created in which transgenes can be inducibly expressed in a lineage-specific manner...
- In vitro culture and transformation of Wolbachia from mosquitoesAnn Fallon; Fiscal Year: 2007..The next challenge to implementation of transgenic mosquitoes is development of tools to drive the spread of transgenes from small numbers of laboratory- manipulated mosquitoes into field populations...
- Wolbachia as an Agent for Population Replacement in Anopheles GambiaeJason Rasgon; Fiscal Year: 2007..b>Transgenes must be actively driven into natural Anopheles populations to high frequency for transgenic strategies to be ..
- THE HORMONAL CONTROL OF FETAL GROWTHA D ERCOLE; Fiscal Year: 1993..Using a number of transgenes designed to alter IGF expression, we will study in utero actions and mechanisms of action of IGFs in cultured ..