expressed sequence tags

Summary

Summary: Partial cDNA (DNA, COMPLEMENTARY) sequences that are unique to the cDNAs from which they were derived.

Top Publications

  1. ncbi High-throughput functional annotation and data mining with the Blast2GO suite
    Stefan Götz
    Bioinformatics Department, Centro de Investigacion Principe Felipe, Valencia, Spain
    Nucleic Acids Res 36:3420-35. 2008
  2. ncbi Rapid transcriptome characterization for a nonmodel organism using 454 pyrosequencing
    J Cristobal Vera
    Department of Biology, 208 Mueller Laboratory, Pennsylvania State University, University Park, PA 16802, USA
    Mol Ecol 17:1636-47. 2008
  3. ncbi Using the miraEST assembler for reliable and automated mRNA transcript assembly and SNP detection in sequenced ESTs
    Bastien Chevreux
    Department of Molecular Biophysics, German Cancer Research Centre Heidelberg, 69120 Heidelberg, Germany
    Genome Res 14:1147-59. 2004
  4. ncbi High-throughput gene and SNP discovery in Eucalyptus grandis, an uncharacterized genome
    Evandro Novaes
    School of Forest Resources and Conservation, University of Florida, PO Box 110410, Gainesville, USA
    BMC Genomics 9:312. 2008
  5. ncbi The genome of black cottonwood, Populus trichocarpa (Torr. & Gray)
    G A Tuskan
    Environmental Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831, USA
    Science 313:1596-604. 2006
  6. ncbi Transcriptome sequencing in an ecologically important tree species: assembly, annotation, and marker discovery
    Thomas L Parchman
    Department of Botany, University of Wyoming, Laramie, WY 82071, USA
    BMC Genomics 11:180. 2010
  7. ncbi Cd-hit: a fast program for clustering and comparing large sets of protein or nucleotide sequences
    Weizhong Li
    Burnham Institute for Medical Research La Jolla, CA 92037, USA
    Bioinformatics 22:1658-9. 2006
  8. ncbi Genic microsatellite markers in plants: features and applications
    Rajeev K Varshney
    Institute of Plant Genetics and Crop Plant Research IPK, Corrensstrasse 3, D 06466 Gatersleben, Germany
    Trends Biotechnol 23:48-55. 2005
  9. ncbi De novo sequencing and analysis of the American ginseng root transcriptome using a GS FLX Titanium platform to discover putative genes involved in ginsenoside biosynthesis
    Chao Sun
    Institute of Medicinal Plant Development IMPLAD, Chinese Academy of Medical Sciences, Haidian District, Beijing, China
    BMC Genomics 11:262. 2010
  10. ncbi TIGR Gene Indices clustering tools (TGICL): a software system for fast clustering of large EST datasets
    Geo Pertea
    The Institute for Genomic Research, Rockville, MD 20850, USA
    Bioinformatics 19:651-2. 2003

Research Grants

  1. Shotgun Sequencing of the Trichomonas Vaginalis Genome
    Jane Carlton; Fiscal Year: 2003
  2. Metabolic Pathways and Defects in Fructose Metabolism
    DEAN TOLAN; Fiscal Year: 2009
  3. Metabolic Pathways and Defects in Fructose Metabolism
    DEAN TOLAN; Fiscal Year: 2007
  4. Genetic Heterogeneity and Protein Function in DBA
    Colin Sieff; Fiscal Year: 2003
  5. The Tetrahymena Genome Project
    Eduardo Orias; Fiscal Year: 2004
  6. Identification of Metastasis-related Gene in Oral Cancer
    Zhuo Chen; Fiscal Year: 2003
  7. A GENOMIC APPROACH TO PARASITES FROM THE PHYLUM NEMATODA
    Makedonka Mitreva; Fiscal Year: 2007
  8. CHROMATIN-TELOMERE STRUCTURE AND AT GENOMIC SENSITIVITY
    TEJ PANDITA; Fiscal Year: 2005
  9. CHARACTERIZING THE ZEBRAFISH GENOME
    Leonard Zon; Fiscal Year: 2002
  10. AUTOSOMAL DOMINANT FOCAL SEGMENTAL GLOMERULOSCLEROSIS
    Michelle Winn; Fiscal Year: 2004

Detail Information

Publications279 found, 100 shown here

  1. ncbi High-throughput functional annotation and data mining with the Blast2GO suite
    Stefan Götz
    Bioinformatics Department, Centro de Investigacion Principe Felipe, Valencia, Spain
    Nucleic Acids Res 36:3420-35. 2008
    ..Our aim is to offer biologists useful information to take into account when addressing the task of functionally characterizing their sequence data...
  2. ncbi Rapid transcriptome characterization for a nonmodel organism using 454 pyrosequencing
    J Cristobal Vera
    Department of Biology, 208 Mueller Laboratory, Pennsylvania State University, University Park, PA 16802, USA
    Mol Ecol 17:1636-47. 2008
    ..DNA collections from a genetically diverse pool of larvae, pupae, and adults yielded 608,053 expressed sequence tags (mean length = 110 nucleotides), which assembled into 48,354 contigs (sets of overlapping DNA segments) ..
  3. ncbi Using the miraEST assembler for reliable and automated mRNA transcript assembly and SNP detection in sequenced ESTs
    Bastien Chevreux
    Department of Molecular Biophysics, German Cancer Research Centre Heidelberg, 69120 Heidelberg, Germany
    Genome Res 14:1147-59. 2004
    ....
  4. ncbi High-throughput gene and SNP discovery in Eucalyptus grandis, an uncharacterized genome
    Evandro Novaes
    School of Forest Resources and Conservation, University of Florida, PO Box 110410, Gainesville, USA
    BMC Genomics 9:312. 2008
    ..However, it is questionable how effective the sequencing of large numbers of short reads for species with essentially no prior gene sequence information will support contig assemblies and sequence annotation...
  5. ncbi The genome of black cottonwood, Populus trichocarpa (Torr. & Gray)
    G A Tuskan
    Environmental Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831, USA
    Science 313:1596-604. 2006
    ..Overrepresented exceptions in Populus include genes associated with lignocellulosic wall biosynthesis, meristem development, disease resistance, and metabolite transport...
  6. ncbi Transcriptome sequencing in an ecologically important tree species: assembly, annotation, and marker discovery
    Thomas L Parchman
    Department of Botany, University of Wyoming, Laramie, WY 82071, USA
    BMC Genomics 11:180. 2010
    ..Here we describe a sequencing study of expressed genes from P. contorta, including their assembly and annotation, and their potential for molecular marker development to support population and association genetic studies...
  7. ncbi Cd-hit: a fast program for clustering and comparing large sets of protein or nucleotide sequences
    Weizhong Li
    Burnham Institute for Medical Research La Jolla, CA 92037, USA
    Bioinformatics 22:1658-9. 2006
    ..All these programs can handle huge datasets with millions of sequences and can be hundreds of times faster than methods based on the popular sequence comparison and database search tools, such as BLAST...
  8. ncbi Genic microsatellite markers in plants: features and applications
    Rajeev K Varshney
    Institute of Plant Genetics and Crop Plant Research IPK, Corrensstrasse 3, D 06466 Gatersleben, Germany
    Trends Biotechnol 23:48-55. 2005
    ..Applications and potential uses of EST-SSRs in plant genetics and breeding are discussed...
  9. ncbi De novo sequencing and analysis of the American ginseng root transcriptome using a GS FLX Titanium platform to discover putative genes involved in ginsenoside biosynthesis
    Chao Sun
    Institute of Medicinal Plant Development IMPLAD, Chinese Academy of Medical Sciences, Haidian District, Beijing, China
    BMC Genomics 11:262. 2010
    ..Its major bioactive constituents are the triterpene saponins known as ginsenosides. However, little is known about ginsenoside biosynthesis in American ginseng, especially the late steps of the pathway...
  10. ncbi TIGR Gene Indices clustering tools (TGICL): a software system for fast clustering of large EST datasets
    Geo Pertea
    The Institute for Genomic Research, Rockville, MD 20850, USA
    Bioinformatics 19:651-2. 2003
    TGICL is a pipeline for analysis of large Expressed Sequence Tags (EST) and mRNA databases in which the sequences are first clustered based on pairwise sequence similarity, and then assembled by individual clusters (optionally with ..
  11. ncbi Exploiting EST databases for the development and characterization of gene-derived SSR-markers in barley (Hordeum vulgare L.)
    T Thiel
    Institute of Plant Genetics and Crop Plant Research (IPK, Corrensstrasse 3, D-06466 Gatersleben, Germany
    Theor Appl Genet 106:411-22. 2003
    ....
  12. ncbi De novo transcriptome sequencing in Anopheles funestus using Illumina RNA-seq technology
    Jacob E Crawford
    Department of Entomology, Cornell University, Ithaca, New York, United States of America
    PLoS ONE 5:e14202. 2010
    ..funestus from a newly founded colony in Burkina Faso, West Africa, using the Illumina GAIIx next generation sequencing platform...
  13. ncbi Global characterization of Artemisia annua glandular trichome transcriptome using 454 pyrosequencing
    Wei Wang
    Department of Biology and the State Key Lab for Agrobiotechnology, The Chinese University of Hong Kong, Hong Kong SAR, PR China
    BMC Genomics 10:465. 2009
    ..The most prominent anti-malarial drug artemisinin, a sesquiterpene lactone, is produced in glandular trichomes of Artemisia annua. However, only limited genomic information is currently available in this non-model plant species...
  14. ncbi Transcriptome sequencing and comparative analysis of cucumber flowers with different sex types
    Shaogui Guo
    1National Engineering Research Center for Vegetables, Beijing 100097, China
    BMC Genomics 11:384. 2010
    ....
  15. ncbi Tissue-specific transcriptomics of the exotic invasive insect pest emerald ash borer (Agrilus planipennis)
    Omprakash Mittapalli
    Department of Entomology, Ohio Agricultural and Research Development Center, The Ohio State University, Wooster, Ohio, United States of America
    PLoS ONE 5:e13708. 2010
    ..primarily in the Midwestern United States and Ontario, Canada. However, despite its high impact status little knowledge exists for A. planipennis at the molecular level...
  16. ncbi Development of a EST dataset and characterization of EST-SSRs in a traditional Chinese medicinal plant, Epimedium sagittatum (Sieb. Et Zucc.) Maxim
    Shaohua Zeng
    Key Laboratory of Pant Germplasm Enhancement and Speciality Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, Hubei 430074, China
    BMC Genomics 11:94. 2010
    ..Here, we report a set of expressed sequence tags (ESTs) and simple sequence repeats (SSRs) identified in these ESTs for E. sagittatum.
  17. ncbi GENCODE: producing a reference annotation for ENCODE
    Jennifer Harrow
    Wellcome Trust Sanger Institute, Wellcome Trust Campus, Hinxton, Cambridge CB10 1SA, UK
    Genome Biol 7:S4.1-9. 2006
    ..This was achieved by a combination of initial manual annotation by the HAVANA team, experimental validation by the GENCODE consortium and a refinement of the annotation based on these experimental results...
  18. ncbi Comparison of the transcriptomes of American chestnut (Castanea dentata) and Chinese chestnut (Castanea mollissima) in response to the chestnut blight infection
    Abdelali Barakat
    The School of Forest Resources, Department of Horticulture, The Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, PA 16802, USA
    BMC Plant Biol 9:51. 2009
    ..We also identified several candidate genes that may underline the difference in resistance to Cryphonectria parasitica between American chestnut and Chinese chestnut...
  19. ncbi Generation and analysis of a 29,745 unique Expressed Sequence Tags from the Pacific oyster (Crassostrea gigas) assembled into a publicly accessible database: the GigasDatabase
    Elodie Fleury
    UMR M100 Ifremer Université de Caen Basse Normandie Physiologie et Ecophysiologie des Mollusques Marins, Centre de Brest, B, P, 70, 29280 Plouzané IBFA, IFR ICORE 146, Esplanade de la Paix, 14032 Caen Cedex, France
    BMC Genomics 10:341. 2009
    ..cDNA sequencing projects for the Pacific oyster Crassostrea gigas initiated to provide a large number of expressed sequence tags that were subsequently compiled in a publicly accessible database...
  20. ncbi In silico analysis on frequency and distribution of microsatellites in ESTs of some cereal species
    Rajeev K Varshney
    Institute of Plant Genetics and Crop Plant Research (IPK, Corrensstrasse 3, D 06466 Gatersleben, Germany
    Cell Mol Biol Lett 7:537-46. 2002
    ..sequencing and genomics programmes in various cereal species at different laboratories, a large set of expressed sequence tags (ESTs) is being generated, which can be used to search for microsatellites...
  21. ncbi GMAP: a genomic mapping and alignment program for mRNA and EST sequences
    Thomas D Wu
    Department of Bioinformatics Genentech, Inc, South San Francisco, CA 94080, USA
    Bioinformatics 21:1859-75. 2005
    ....
  22. ncbi Phylogenomics reshuffles the eukaryotic supergroups
    Fabien Burki
    Department of Zoology and Animal Biology, University of Geneva, Geneva, Switzerland
    PLoS ONE 2:e790. 2007
    ..Yet, the composition of these large assemblages and their relationships remain controversial...
  23. ncbi An oligo-based microarray offers novel transcriptomic approaches for the analysis of pathogen resistance and fruit quality traits in melon (Cucumis melo L.)
    Albert Mascarell-Creus
    Molecular Genetics Department, Centre for Research in Agricultural Genomics CRAG CSIC IRTA UAB, Barcelona 08034, Spain
    BMC Genomics 10:467. 2009
    ..However, the unprecedented existence of a transcriptomic approaches in melon, highlight the importance of designing new tools for high-throughput analysis of gene expression...
  24. ncbi Comparative 454 pyrosequencing of transcripts from two olive genotypes during fruit development
    Fiammetta Alagna
    CNR Institute of Plant Genetics, Via Madonna Alta 130, 06128 Perugia, Italy
    BMC Genomics 10:399. 2009
    ..454 pyrosequencing was used to enrich the very few sequence data currently available for the Olea europaea species and to identify genes involved in expression of fruit quality traits...
  25. ncbi Gene discovery and annotation using LCM-454 transcriptome sequencing
    Scott J Emrich
    Bioinformatics and Computational Biology Graduate Program, Iowa State University, Ames, IA 50010, USA
    Genome Res 17:69-73. 2007
    ..We conclude that the coupling of LCM and 454 sequencing technologies facilitates the discovery of rare, possibly cell-type-specific transcripts...
  26. ncbi A consensus genetic map of cowpea [Vigna unguiculata (L) Walp.] and synteny based on EST-derived SNPs
    Wellington Muchero
    Departments of Nematology and Botany and Plant Sciences, University of California, Riverside, CA 92521, USA
    Proc Natl Acad Sci U S A 106:18159-64. 2009
    ..These results support evolutionary closeness between cowpea and soybean and identify regions for synteny-based functional genomics studies in legumes...
  27. ncbi Single nucleotide polymorphism (SNP) discovery in the polyploid Brassica napus using Solexa transcriptome sequencing
    Martin Trick
    John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, UK
    Plant Biotechnol J 7:334-46. 2009
    ..of a polyploid crop, and the Solexa sequencing system was used to generate approximately 20 million expressed sequence tags (ESTs) from each of two cultivars: Tapidor and Ningyou 7...
  28. ncbi Transcriptome profiling of a toxic dinoflagellate reveals a gene-rich protist and a potential impact on gene expression due to bacterial presence
    Ahmed Moustafa
    Ecology, Evolution and Natural Resources, Institute of Marine and Coastal Sciences, Rutgers, The State University of New Jersey, New Brunswick, New Jersey, United States of America
    PLoS ONE 5:e9688. 2010
    ..Here, we used massively parallel signature sequencing (MPSS) to understand global transcriptional regulation patterns in Alexandrium tamarense cultures that were grown under four different conditions...
  29. ncbi Synteny mapping between common bean and soybean reveals extensive blocks of shared loci
    Phillip E McClean
    Genomics and Bioinformatics Program, North Dakota State University, Fargo, ND 58105, USA
    BMC Genomics 11:184. 2010
    ..Determining the syntentic relationship between these two species will allow researchers to leverage not only genomic resources but also genetic data for important agronomic traits to improve both of these species...
  30. ncbi Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization
    Caroline Joubert
    Ifremer Laboratoire de Biotechnologie et de Qualité de Perle, Centre Océanologique du Pacifique BP 7004, 98719 Taravao, French Polynesia
    BMC Genomics 11:613. 2010
    ..margaritifera, high-throughput Expressed Sequence Tag (EST) pyrosequencing was undertaken on the calcifying mantle, combined with a proteomic analysis of the shell...
  31. ncbi High-density integrated linkage map based on SSR markers in soybean
    Tae Young Hwang
    National Agricultural Research Center for Hokkaido Region, Sapporo, Hokkaido 062 8555, Japan
    DNA Res 16:213-25. 2009
    ..8. Our high-density linkage map should facilitate ongoing and future genomic research such as analysis of quantitative trait loci and positional cloning in addition to marker-assisted selection in soybean breeding...
  32. ncbi Microarray analysis identifies candidate genes for key roles in coral development
    Lauretta C Grasso
    Centre for the Molecular Genetics of Development, Research School of Biological Sciences, Australian National University, Canberra, Australia
    BMC Genomics 9:540. 2008
    ....
  33. ncbi Nonrandom distribution and frequencies of genomic and EST-derived microsatellite markers in rice, wheat, and barley
    Mauricio La Rota
    Department of Plant Breeding and Genetics, 240 Emerson Hall, Cornell University, Ithaca, NY 14853, USA
    BMC Genomics 6:23. 2005
    ..sequences containing simple sequence repeats (SSR or microsatellites) by length and motif for wheat, barley and rice and to determine in-silico rice genome locations for primer sets developed for wheat and barley Expressed Sequence Tags.
  34. ncbi EST-SSRs as a resource for population genetic analyses
    J R Ellis
    Department of Biological Sciences, Vanderbilt University, Nashville, TN 37232, USA
    Heredity (Edinb) 99:125-32. 2007
    ..in the production of vast amounts of publicly available DNA sequence data, including large collections of expressed sequence tags (ESTs) from a variety of different taxa...
  35. ncbi Quality assessment parameters for EST-derived SNPs from catfish
    Shaolin Wang
    The Fish Molecular Genetics and Biotechnology Laboratory, Department of Fisheries and Allied Aquacultures and Program of Cell and Molecular Biosciences, Aquatic Genomics Unit, Auburn University, Auburn, AL 36849, USA
    BMC Genomics 9:450. 2008
    ..This project was designed to answer some of the questions relevant to quality assessment of EST-derived SNPs...
  36. ncbi SNP discovery via 454 transcriptome sequencing
    W Brad Barbazuk
    Donald Danforth Plant Science Center, St Louis, MO 63132, USA
    Plant J 51:910-8. 2007
    ..These results demonstrate that 454-based transcriptome sequencing is an excellent method for the high-throughput acquisition of gene-associated SNPs...
  37. ncbi An approach to transcriptome analysis of non-model organisms using short-read sequences
    Lesley J Collins
    Allan Wilson Centre for Molecular Ecology and Evolution, Massey University, Palmerston North, New Zealand
    Genome Inform 21:3-14. 2008
    ..Our approach demonstrates that transcriptome analysis using high-throughput short-read sequencing need not be restricted to the genomes of model organisms...
  38. ncbi Development and mapping of 2240 new SSR markers for rice (Oryza sativa L.)
    Susan R McCouch
    Plant Breeding Dept, Cornell University, Ithaca, NY 14853 1901, USA
    DNA Res 9:199-207. 2002
    ..In combination with the pool of 500 previously mapped SSR markers, this release makes available a total of 2740 experimentally confirmed SSR markers for rice, or approximately one SSR every 157 kb...
  39. ncbi "Stemness": transcriptional profiling of embryonic and adult stem cells
    Miguel Ramalho-Santos
    Department of Molecular and Cellular Biology and Howard Hughes Medical Institute (HHMI, Harvard University, Cambridge, MA 02138, USA
    Science 298:597-600. 2002
    ..to differentiated cell types, stem cells express a significantly higher number of genes (represented by expressed sequence tags) whose functions are unknown...
  40. ncbi Next generation transcriptomes for next generation genomes using est2assembly
    Alexie Papanicolaou
    Department of Entomology, Max Planck Institute for Chemical Ecology, Jena, Germany
    BMC Bioinformatics 10:447. 2009
    ..This rapid growth in data has outstripped the ability of researchers working on non-model species to analyze and mine transcriptome data efficiently...
  41. ncbi Deep sampling of the Palomero maize transcriptome by a high throughput strategy of pyrosequencing
    Julio C Vega-Arreguín
    Laboratorio Nacional de Genómica para la Biodiversidad, Cinvestav Campus Guanajuato, Carretera Irapuato León, Irapuato, Gto, Mexico
    BMC Genomics 10:299. 2009
    ..In some cases, deep parallel sequencing of Expressed Sequence Tags (ESTs), although not yet optimized for the sequencing of cDNAs, has represented an efficient procedure ..
  42. ncbi A Brassica rapa linkage map of EST-based SNP markers for identification of candidate genes controlling flowering time and leaf morphological traits
    Feng Li
    Laboratory of Plant Breeding and Genetics, Graduate School of Agricultural Science, Tohoku University, 1 1 Tsutsumidori Amamiyamachi, Aoba, Sendai, Miyagi 981 8555, Japan
    DNA Res 16:311-23. 2009
    ..Analysis of nucleotide sequences and expression of these genes suggested possible involvement of these genes in leaf morphological traits...
  43. ncbi The TIGR Rice Genome Annotation Resource: improvements and new features
    Shu Ouyang
    The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA
    Nucleic Acids Res 35:D883-7. 2007
    ..Enhanced data access is available through web interfaces, FTP downloads and a Data Extractor tool developed in order to support discrete dataset downloads...
  44. ncbi Identification and characterization of nucleotide-binding site-leucine-rich repeat genes in the model plant Medicago truncatula
    Carine Ameline-Torregrosa
    Laboratoire des Interactions Plantes Microorganismes, UMR CNRS INRA 442 2594, 31326, Castanet Tolosan, France
    Plant Physiol 146:5-21. 2008
    ..This analysis shows the gene family not only is important functionally and agronomically, but also plays a structural role in the genome...
  45. ncbi Arabidopsis genes involved in acyl lipid metabolism. A 2003 census of the candidates, a study of the distribution of expressed sequence tags in organs, and a web-based database
    Frédéric Beisson
    Department of Plant Biology, Michigan State University, East Lansing 48824, USA
    Plant Physiol 132:681-97. 2003
    ....
  46. ncbi OrchidBase: a collection of sequences of the transcriptome derived from orchids
    Chih Hsiung Fu
    Department of Engineering Science, National Cheng Kung University, Tainan 701, Taiwan
    Plant Cell Physiol 52:238-43. 2011
    ..Among them, 41,310 expressed sequence tags (ESTs) were obtained by using Sanger sequencing, whereas 37,908,032 reads were obtained by using next-..
  47. ncbi A high-density transcript linkage map of barley derived from a single population
    K Sato
    Research Institute for Bioresources, Okayama University, Chuo, Kurashiki, Japan
    Heredity (Edinb) 103:110-7. 2009
    ..linkage map of barley was created using a single doubled haploid (DH) mapping population, only 3'-end expressed sequence tags (ESTs) and only PCR-based assays...
  48. ncbi Computational identification of novel microRNAs and targets in Brassica napus
    Fu Liang Xie
    Department of Biochemistry and Molecular Biology, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China
    FEBS Lett 581:1464-74. 2007
    ..We used previously known miRNAs from Arabidopsis, rice and other plant species against both expressed sequence tags (EST) and genomic survey sequence (GSS) databases to search for potential miRNAs in B. napus...
  49. ncbi Gene discovery using massively parallel pyrosequencing to develop ESTs for the flesh fly Sarcophaga crassipalpis
    Daniel A Hahn
    Department of Entomology and Nematology, The University of Florida, Gainesville, FL 32611 0620, USA
    BMC Genomics 10:234. 2009
    ..To maximize sequence diversity, we pooled RNA extracted from whole bodies of all life stages and normalized the cDNA pool after reverse transcription...
  50. ncbi A comprehensive resource of drought- and salinity- responsive ESTs for gene discovery and marker development in chickpea (Cicer arietinum L.)
    Rajeev K Varshney
    International Crops Research Institute for the Semi Arid Tropics ICRISAT, Patancheru, Greater Hyderabad, AP, India
    BMC Genomics 10:523. 2009
    ..This study reports generation and analysis of comprehensive resource of drought- and salinity-responsive expressed sequence tags (ESTs) and gene-based markers.
  51. ncbi QualitySNP: a pipeline for detecting single nucleotide polymorphisms and insertions/deletions in EST data from diploid and polyploid species
    Jifeng Tang
    Plant Research International, PO Box 16, 6700 AA Wageningen, The Netherlands
    BMC Bioinformatics 7:438. 2006
    ..for SNP discovery make use of the large number of sequences present in public databases (in most cases as expressed sequence tags (ESTs)) and are considered to be faster and more cost-effective than experimental procedures...
  52. ncbi Pyrosequencing the Manduca sexta larval midgut transcriptome: messages for digestion, detoxification and defence
    Y Pauchet
    School of Biosciences, University of Exeter, Cornwall Campus, Penryn, UK
    Insect Mol Biol 19:61-75. 2010
    ..Following a recent pyrosequencing study generating immune related expressed sequence tags (ESTs), here we use this new technology to define the M. sexta larval midgut transcriptome...
  53. ncbi Transcriptional regulation of biomass-degrading enzymes in the filamentous fungus Trichoderma reesei
    Pamela K Foreman
    Genencor International, Inc, Palo Alto, California 94304, USA
    J Biol Chem 278:31988-97. 2003
    ..However, genes whose products are involved in protein processing and secretion were not highly regulated during cellulase induction...
  54. ncbi High throughput SNP discovery and genotyping in grapevine (Vitis vinifera L.) by combining a re-sequencing approach and SNPlex technology
    Diego Lijavetzky
    Departamento de Genética Molecular de Plantas, Centro Nacional de Biotecnologia, Consejo Superior de Investigaciones Cientificas CSIC, C Darwin 3, 28049 Madrid, Spain
    BMC Genomics 8:424. 2007
    ..b>Expressed sequence tags (EST) sequencing projects in grapevine are allowing for the in silico detection of multiple putative ..
  55. ncbi Conservation and divergence of plant microRNA genes
    Baohong Zhang
    The Institute of Environmental and Human Health (TIEHH, and Department of Environmental Toxicology, Texas Tech University, Lubbock, TX 79409-1163, USA
    Plant J 46:243-59. 2006
    ..Regulation of gene expression by miRNAs appears to have existed at the earliest stages of plant evolution and has been tightly constrained (functionally) for more than 425 million years...
  56. ncbi Identification of soybean microRNAs and their targets
    Baohong Zhang
    Department of Biology, East Carolina University, Greenville, NC 27858, USA
    Planta 229:161-82. 2008
    ....
  57. ncbi Combining next-generation pyrosequencing with microarray for large scale expression analysis in non-model species
    Diana Bellin
    Department of Biotechnology, University of Verona, Strada Le Grazie 15, 37134 Verona, Italy
    BMC Genomics 10:555. 2009
    ..We describe here an innovative approach for this purpose and demonstrate its potential also for non-model species...
  58. ncbi Identification, analysis, and utilization of conserved ortholog set markers for comparative genomics in higher plants
    Theresa M Fulton
    Department of Plant Breeding, Cornell University, Ithaca, New York 14853, USA
    Plant Cell 14:1457-67. 2002
    ..This set of COS markers, identified computationally and experimentally, may further studies on comparative genomes and phylogenetics and elucidate the nature of genes conserved throughout plant evolution...
  59. ncbi MytiBase: a knowledgebase of mussel (M. galloprovincialis) transcribed sequences
    Paola Venier
    Department of Biology, University of Padova, Via U Bassi 58 B, Padova, Italy
    BMC Genomics 10:72. 2009
    ....
  60. ncbi From stop to start: tandem gene arrangement, copy number and trans-splicing sites in the dinoflagellate Amphidinium carterae
    Tsvetan R Bachvaroff
    Center of Marine Biotechnology, University of Maryland Biotechnology Institute, Baltimore, Maryland, United States of America
    PLoS ONE 3:e2929. 2008
    ..This combination of features appears to be unique among eukaryotes...
  61. ncbi Insights into corn genes derived from large-scale cDNA sequencing
    Nickolai N Alexandrov
    Ceres, Inc, 1535 Rancho Conejo Blvd, Thousand Oaks, CA 91320, USA
    Plant Mol Biol 69:179-94. 2009
    ..All of the sequence data from this study have been submitted to DDBJ/GenBank/EMBL under accession numbers EU940701-EU977132 (FLI cDNA) and FK944382-FL482108 (EST)...
  62. ncbi Genomic resources for Myzus persicae: EST sequencing, SNP identification, and microarray design
    John S Ramsey
    Boyce Thompson Institute for Plant Research, Tower Road, Ithaca, NY 14853, USA
    BMC Genomics 8:423. 2007
    ..However, despite the significant damage inflicted by M. persicae in agricultural systems through direct feeding damage and by its ability to transmit plant viruses, limited genomic information is available for this species...
  63. ncbi A conifer genomics resource of 200,000 spruce (Picea spp.) ESTs and 6,464 high-quality, sequence-finished full-length cDNAs for Sitka spruce (Picea sitchensis)
    Steven G Ralph
    Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia, Canada
    BMC Genomics 9:484. 2008
    ..Sequence-finished full-length (FL)cDNAs and large collections of expressed sequence tags (ESTs) are essential for gene discovery, functional genomics, and for future efforts of conifer genome ..
  64. ncbi Identification and functional characterization of effectors in expressed sequence tags from various life cycle stages of the potato cyst nematode Globodera pallida
    John T Jones
    Plant Pathology Programme, SCRI, Invergowrie, Dundee DD2 5DA, UK
    Mol Plant Pathol 10:815-28. 2009
    In this article, we describe the analysis of over 9000 expressed sequence tags (ESTs) from cDNA libraries obtained from various life cycle stages of Globodera pallida. We have identified over 50 G...
  65. ncbi Comparison of 454-ESTs from Huperzia serrata and Phlegmariurus carinatus reveals putative genes involved in lycopodium alkaloid biosynthesis and developmental regulation
    Hongmei Luo
    Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Haidian District, Beijing, China
    BMC Plant Biol 10:209. 2010
    ..We focused on gene discovery in the areas of bioactive compound biosynthesis and transcriptional regulation as well as genetic marker detection in these species...
  66. ncbi Comprehensive EST analysis of the symbiotic sea anemone, Anemonia viridis
    Cécile Sabourault
    Universite de Nice Sophia Antipolis, EA4228 ECOMERS, Nice, France
    BMC Genomics 10:333. 2009
    ....
  67. ncbi A high density barley microsatellite consensus map with 775 SSR loci
    R K Varshney
    Leibniz Institute of Plant Genetics and Crop Plant Research IPK, Corrensstrasse 3, 06466 Gatersleben, Germany
    Theor Appl Genet 114:1091-103. 2007
    ..This map also offers the possibilities of thorough alignment for the (future) physical map and implementation in haplotype diversity studies of barley...
  68. ncbi 'Green revolution' genes encode mutant gibberellin response modulators
    J Peng
    John Innes Centre, Norwich Research Park, UK
    Nature 400:256-61. 1999
    ..Transgenic rice plants containing a mutant GAI allele give reduced responses to gibberellin and are dwarfed, indicating that mutant GAI orthologues could be used to increase yield in a wide range of crop species...
  69. ncbi Bin mapping of genomic and EST-derived SSRs in melon (Cucumis melo L.)
    I Fernández-Silva
    IRTA, Centre de Recerca en Agrigenòmica CSIC IRTA UAB CRAG, Carretera de Cabrils, Km 2, 08348, Cabrils, Barcelona, Spain
    Theor Appl Genet 118:139-50. 2008
    ..A final bin-map was constructed including 80 RFLPs, 212 SSRs, 3 SNPs and the Nsv locus, distributed in 122 bins with an average bin length of 10.2 cM and a maximum bin length of 33 cM. Map density was 4.2 cM/marker or 5.9 cM/SSR...
  70. ncbi dCAS: a desktop application for cDNA sequence annotation
    Yongjian Guo
    Bioinformatics and Computational Biosciences Branch, Office of Cyber Infrastructure and Computational Biology and Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892, USA
    Bioinformatics 25:1195-6. 2009
    ..AVAILABILITY: The dCAS package is distributed freely. A cross-platform installer and associated sequence databases can be downloaded at: http://exon.niaid.nih.gov/applications.html...
  71. ncbi A genetic map of melon highly enriched with fruit quality QTLs and EST markers, including sugar and carotenoid metabolism genes
    R Harel-Beja
    Department of Vegetable Research, Agricultural Research Organization, Newe Ya ar Research Center, P O Box 1021, Ramat Yishay, 30095, Israel
    Theor Appl Genet 121:511-33. 2010
    ..The RI population described here provides a useful resource for further genomics and metabolomics studies in melon, as well as useful markers for breeding for fruit quality...
  72. ncbi LocustDB: a relational database for the transcriptome and biology of the migratory locust (Locusta migratoria)
    Zongyuan Ma
    National Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China
    BMC Genomics 7:11. 2006
    ..We developed the first transcriptomic database of the locust (LocustDB), building necessary infrastructures to integrate, organize, and retrieve data that are either currently available or to be acquired in the future...
  73. ncbi Characterization of the soybean genome using EST-derived microsatellite markers
    Hiroshi Hisano
    Kazusa DNA Research Institute, 2 6 7 Kazusa Kamatari, Kisarazu, Chiba 292 0818, Japan
    DNA Res 14:271-81. 2007
    ....
  74. ncbi MELOGEN: an EST database for melon functional genomics
    Daniel Gonzalez-Ibeas
    Departamento de Biología del Estrés y Patología Vegetal, Centro de Edafologia y Biologia Aplicada del Segura CEBAS CSIC, Apdo, correos 164, 30100 Espinardo Murcia, Spain
    BMC Genomics 8:306. 2007
    ..up the process of breeding new and better adapted melon varieties, we have produced a large collection of expressed sequence tags (ESTs) from eight normalized cDNA libraries from different tissues in different physiological conditions.
  75. ncbi Genome structure of cotton revealed by a genome-wide SSR genetic map constructed from a BC1 population between gossypium hirsutum and G. barbadense
    Yu Yu
    National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research Wuhan, Huazhong Agricultural University, Wuhan 430070, Hubei, PR China
    BMC Genomics 12:15. 2011
    ..To construct a genome-wide, functional marker-based genetic linkage map in cotton, we isolated and mapped expressed sequence tag-simple sequence repeats (EST-SSRs) from cotton ESTs derived from the A1, D5, (AD)1, and (AD)2 genome...
  76. ncbi Carbohydrate mobilization and gene regulatory profile in the pseudobulb of Oncidium orchid during the flowering process
    Chih Yu Wang
    Institute of Plant Biology, National Taiwan University, Taipei, Taiwan
    Planta 227:1063-77. 2008
    ..These results demonstrated a novel functional profile of polysaccharide mobilization pathway as well as their relevant gene expression in the pseudobulb of Oncidium orchid during the flowering process...
  77. ncbi SNP discovery and linkage map construction in cultivated tomato
    Kenta Shirasawa
    Kazusa DNA Research Institute, 2 6 7 Kazusa Kamatari, Kisarazu, Chiba 292 0818, Japan
    DNA Res 17:381-91. 2010
    ..In this study, we developed SNP markers based on expressed sequence tags for the construction of intraspecific linkage maps in tomato...
  78. ncbi Analysis of organ-specific, expressed genes in Oncidium orchid by subtractive expressed sequence tags library
    Jun Tan
    Institute of Plant Biology, National Taiwan University, 106, Taipei, Taiwan
    Biotechnol Lett 27:1517-28. 2005
    ..In total, 1080 subtractive expressed sequence tags (ESTs) were obtained. Analysis revealed approximately 636 unique gene parts, 120 clusters and 516 singles...
  79. ncbi Development and analysis of EST-SSRs for flax (Linum usitatissimum L.)
    Sylvie Cloutier
    Cereal Research Centre, Agriculture and Agri Food Canada, Winnipeg, MB, Canada
    Theor Appl Genet 119:53-63. 2009
    A set of 146,611 expressed sequence tags (ESTs) were generated from 10 flax cDNA libraries...
  80. ncbi Analysis of expressed sequence tags from a single wheat cultivar facilitates interpretation of tandem mass spectrometry data and discrimination of gamma gliadin proteins that may play different functional roles in flour
    Susan B Altenbach
    USDA ARS Western Regional Research Center, U S Department of Agriculture Agricultural Research Service, 800 Buchanan Street, Albany, CA 94710, USA
    BMC Plant Biol 10:7. 2010
    ..The ability to differentiate among the gamma gliadins is important for studies of wheat flour quality because proteins with similar sequences can have different effects on functional properties...
  81. ncbi Comparative analysis of secreted protein evolution using expressed sequence tags from four poplar leaf rusts (Melampsora spp.)
    David L Joly
    Natural Resources Canada, Canadian Forest Service, Laurentian Forestry Centre, 1055 du PEPS, P, O, Box 10380, Stn, Sainte Foy, Quebec, QC, G1V 4C7, Canada
    BMC Genomics 11:422. 2010
    ..Recently, whole genome sequence data has allowed the prediction of secretomes, facilitating the identification of putative effectors...
  82. ncbi Genes expression analyses of sea-island cotton (Gossypium barbadense L.) during fiber development
    Li Li Tu
    National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, Hubei 430070, China
    Plant Cell Rep 26:1309-20. 2007
    ..The 887 high-quality expressed sequence tags obtained were assembled into 645 consensus sequences (582 singletons and 63 contigs), of which 455 were ..
  83. ncbi Mining an Ostrinia nubilalis midgut expressed sequence tag (EST) library for candidate genes and single nucleotide polymorphisms (SNPs)
    B S Coates
    USDA ARS, Corn Insect and Crop Genetics Research Unit, Genetics Laboratory, Iowa State University, Ames, Iowa 50011, USA
    Insect Mol Biol 17:607-20. 2008
    ..nubilalis larval midgut expressed sequence tags (ESTs). Full-length cDNA sequence of 12 putative serine proteinase genes and 3 partial O...
  84. ncbi Phylogenomic analyses support the monophyly of Taphrinomycotina, including Schizosaccharomyces fission yeasts
    Yu Liu
    Robert Cedergren Centre, Departement de Biochimie, Universite de Montreal, Montreal, Quebec, Canada
    Mol Biol Evol 26:27-34. 2009
    ....
  85. ncbi Gene expression in Florida red tide dinoflagellate Karenia brevis: analysis of an expressed sequence tag library and development of DNA microarray
    Kristy B Lidie
    Marine Biotoxins Program, NOAA Center for Coastal Environmental and Biomolecular Research, SC 29412, USA
    Mar Biotechnol (NY) 7:481-93. 2005
    ..Large-scale sequencing yielded 7001 high-quality expressed sequence tags (ESTs), which clustered into 5280 unique gene groups...
  86. ncbi Parallel evolution of nacre building gene sets in molluscs
    Daniel J Jackson
    School of Biological Sciences, University of Queensland, Brisbane, Australia
    Mol Biol Evol 27:591-608. 2010
    ..The expression of novel biomineralizing RLCD proteins in each of these two molluscs and, interestingly, sea urchins suggests that the evolution of such structural proteins has occurred independently multiple times in the Metazoa...
  87. ncbi A soybean transcript map: gene distribution, haplotype and single-nucleotide polymorphism analysis
    Ik Young Choi
    Soybean Genomics and Improvement Laboratory, USDA, ARS, Beltsville, Maryland 20705, USA
    Genetics 176:685-96. 2007
    ....
  88. ncbi Genetic mapping of EST-derived microsatellites from the diploid Gossypium arboreum in allotetraploid cotton
    Z-G Han
    National Key Laboratory of Crop Genetics and Germplasm Enhancement, Cotton Research Institute, Nanjing Agricultural University, 210095 Nanjing, China
    Mol Genet Genomics 272:308-27. 2004
    ..genomics to elucidate fiber development and breeding in cotton, we sampled microsatellite sequences from expressed sequence tags (ESTs) transcribed during fiber elongation in the A-genome species Gossypium arboreum to evaluate their ..
  89. ncbi Complementary DNA sequencing and identification of mRNAs from the venomous gland of Agkistrodon piscivorus leucostoma
    Ying Jia
    Natural Toxins Research Center, College of Arts and Sciences, Texas A and M University Kingsville, Kingsville, TX 78363, USA
    Toxicon 51:1457-66. 2008
    ..a cDNA library from the venom gland of Agkistrodon piscivorus leucostoma for the generation of expressed sequence tags (ESTs) database...
  90. ncbi Gene cataloging and expression profiling in human gastric cancer cells by expressed sequence tags
    Nam-Soon Kim
    Laboratory of Human Genomics, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-333, South Korea
    Genomics 83:1024-45. 2004
    ..This comprehensive molecular profiling of human gastric cancer should be useful for elucidating the genetic events associated with human gastric cancer...
  91. ncbi An annotated cDNA library and microarray for large-scale gene-expression studies in the ant Solenopsis invicta
    John Wang
    Department of Ecology and Evolution, University of Lausanne, 1015 Lausanne, Switzerland
    Genome Biol 8:R9. 2007
    ..From a normalized cDNA library we obtained 21,715 ESTs, which represent 11,864 putatively different transcripts with very diverse molecular functions. All ESTs were used to construct a cDNA microarray...
  92. ncbi Identification of differentially expressed transcripts from maturing stem of sugarcane by in silico analysis of stem expressed sequence tags and gene expression profiling
    Rosanne E Casu
    CSIRO Plant Industry, Queensland Bioscience Precinct, 306 Carmody Road, St Lucia, Queensland 4067, Australia
    Plant Mol Biol 54:503-17. 2004
    ....
  93. ncbi Analysis and functional annotation of expressed sequence tags from the fall armyworm Spodoptera frugiperda
    Youping Deng
    Molecular, Cellular, and Developmental Biology Program, Division of Biology, Kansas State University, Manhattan, Kansas 66506, USA
    BMC Genomics 7:264. 2006
    ..This study reports the results of an expressed sequence tag (EST) sequencing project in cells from the lepidopteran insect S. frugiperda, the fall armyworm...
  94. ncbi cDNA array analysis of Japanese quail lines divergently selected for four-week body weight
    I W Mott
    Department of Genetics, University of Georgia, Athens, Georgia 30602-7223, USA
    Poult Sci 83:1524-9. 2004
    ..These limited findings suggest that anonymous cDNA array analysis is a productive means to identify differentially expressed genes in growth-selected poultry...
  95. ncbi Generation, annotation and analysis of ESTs from Trichoderma harzianum CECT 2413
    Juan Antonio Vizcaino
    IBVF CIC Isla de la Cartuja, CSIC Universidad de Sevilla, Avda, Americo Vespucio s n, 41092, Sevilla, Spain
    BMC Genomics 7:193. 2006
    ..of this fungus, a functional genomics project called "TrichoEST" was developed to give insights into genes involved in biological control activities using an approach based on the generation of expressed sequence tags (ESTs).
  96. ncbi Automated SNP detection from a large collection of white spruce expressed sequences: contributing factors and approaches for the categorization of SNPs
    Nathalie Pavy
    Forest Genomics, Pavillon Charles Eugène Marchand, Universite Laval, Ste Foy, Quebec G1K 7P4, Canada
    BMC Genomics 7:174. 2006
    ..As a first step toward this goal, we aimed to develop a resource of candidate Single Nucleotide Polymorphisms (SNP) in white spruce (Picea glauca [Moench] Voss), a softwood tree of major economic importance...
  97. ncbi A survey of gene expression and diversity in the venom glands of the pitviper snake Bothrops insularis through the generation of expressed sequence tags (ESTs)
    Inacio de L M Junqueira-de-Azevedo
    Centro de Biotecnologia, Instituto Butantan, Av Vital Brazil, 1500, Sao Paulo, SP 05503 900, Brazil
    Gene 299:279-91. 2002
    ..to RNA library from the venom glands of the Viperidae snake Bothrops insularis for the generation of expressed sequence tags (ESTs)...
  98. ncbi Subtractive hybridization reveals the expression of immunoglobulin-like transcript 7, Eph-B1, granzyme B, and 3 novel transcripts in human plasmacytoid dendritic cells
    Marie Clotilde Rissoan
    Laboratory for Immunological Research, Schering Plough, 27 chemin des Peupliers, BP11, 69571 Dardilly, France
    Blood 100:3295-303. 2002
    ..The identification of genes expressed in PDCs provides new insights into their function and origin...
  99. ncbi Generation and analysis of large-scale expressed sequence tags (ESTs) from a full-length enriched cDNA library of porcine backfat tissue
    Tae Hun Kim
    Division of Animal Genomics and Bioinformatics, National Livestock Research Institute, Rural Development Administration, Omokchun dong 564, Suwon, Korea
    BMC Genomics 7:36. 2006
    ..A combination of quantitative trait locus mapping and microarray analysis is a useful approach to reduce the overall effort needed to identify genes associated with quantitative traits of interest...
  100. ncbi Expressed sequence tag analysis of adult human lens for the NEIBank Project: over 2000 non-redundant transcripts, novel genes and splice variants
    Graeme Wistow
    Section on Molecular Structure and Function, National Eye Institute, National Institutes of Health, Bethesda, MD 20892 2740, USA
    Mol Vis 8:171-84. 2002
    ..To explore the expression profile of the human lens and to provide a resource for microarray studies, expressed sequence tag (EST) analysis has been performed on cDNA libraries from adult lenses...
  101. ncbi Expressed sequence tag analysis of adult human iris for the NEIBank Project: steroid-response factors and similarities with retinal pigment epithelium
    Graeme Wistow
    Section on Molecular Structure and Function, National Eye Institute, National Institutes of Health, Bethesda, MD 20892 2740, USA
    Mol Vis 8:185-95. 2002
    ..Expressed sequence tag (EST) analysis of human iris has been performed to explore the repertoire of genes expressed in this tissue...

Research Grants68

  1. Shotgun Sequencing of the Trichomonas Vaginalis Genome
    Jane Carlton; Fiscal Year: 2003
    ..In addition, we will generate 30,000 Expressed Sequence Tags (ESTs) to aid in gene identification...
  2. Metabolic Pathways and Defects in Fructose Metabolism
    DEAN TOLAN; Fiscal Year: 2009
    ..The large database of expressed sequence tags (dbEST) will be analyzed for overlapping expression profiles of the GLUT5, GLUT2, ketohexokinase, ..
  3. Metabolic Pathways and Defects in Fructose Metabolism
    DEAN TOLAN; Fiscal Year: 2007
    ..The large database of expressed sequence tags (dbEST) will be analyzed for overlapping expression profiles of the GLUT5, GLUT2, ketohexokinase, ..
  4. Genetic Heterogeneity and Protein Function in DBA
    Colin Sieff; Fiscal Year: 2003
    ..screen by cytogenetic techniques for deletions and translocations; (2) use cDNA arrays of the genes and expressed sequence tags (ESTs) in the critical region to define candidate genes by examining their pattern of expressed RNAs in ..
  5. The Tetrahymena Genome Project
    Eduardo Orias; Fiscal Year: 2004
    ..4,000 expressed sequence tags (ESTs) will constitute the backbone of the physical map...
  6. Identification of Metastasis-related Gene in Oral Cancer
    Zhuo Chen; Fiscal Year: 2003
    ..The preliminary experiments revealed the existence of several genes and expressed sequence tags whose expressions were significantly altered in the selected metastatic cell lines...
  7. A GENOMIC APPROACH TO PARASITES FROM THE PHYLUM NEMATODA
    Makedonka Mitreva; Fiscal Year: 2007
    ..University's Genome Sequencing Center (GSC) has generated and immediately made public over 210,000 expressed sequence tags (ESTs) from 25 nematode species, including representatives of all the major groups of human parasites ..
  8. CHROMATIN-TELOMERE STRUCTURE AND AT GENOMIC SENSITIVITY
    TEJ PANDITA; Fiscal Year: 2005
    ..We found six expressed sequence tags aberrantly expressed in Atm null mice as compared to the normal control, and we will characterize them ..
  9. CHARACTERIZING THE ZEBRAFISH GENOME
    Leonard Zon; Fiscal Year: 2002
    ..500 expressed sequence tags (ESTs) derived from cDNA libraries of various tissues and developmental stages will be mapped using this ..
  10. AUTOSOMAL DOMINANT FOCAL SEGMENTAL GLOMERULOSCLEROSIS
    Michelle Winn; Fiscal Year: 2004
    ..PACs and BACs will in turn be used to map expressed sequence tags (ESTs), microsatellites and genes in the MCR...
  11. EFFECTS OF GENE PATENTS ON GENETIC TESTING AND RESEARCH
    Mildred Cho; Fiscal Year: 2002
    ..Many gene sequences, including expressed sequence tags and single nucleotide polymorphisms are particularly useful for developing predictive or diagnostic tests ..
  12. Songbird Neurogenomics Initiative
    David Clayton; Fiscal Year: 2005
    ..a) a collection of cDNA clones representing the mRNAs in the zebra finch brain; b) a database of expressed sequence tags (ESTs) derived by high-throughput sequencing of the collection; c) a web-based publicly accessible server ..
  13. Resources for Teleost Gene Duplicates and Human Disease
    JOHN HARVEY POSTLETHWAIT; Fiscal Year: 2010
    ..Aim 2 is to construct a resource of expressed sequence tags (ESTs) for gar and to develop bioinformatics that assign ESTs to thousands of markers on the linkage map...
  14. Comprehensive Assessment of Endocrine Active Mixtures
    Timothy Zacharewski; Fiscal Year: 2003
    ..cDNA clones or expressed sequence tags (ESTs) for the estrogen responsive genes will be used to construct a custom estrogen responsive cDNA/EST ..