operon

Summary

Summary: In bacteria, a group of metabolically related genes, with a common PROMOTER, whose transcription into a single polycistronic MESSENGER RNA is under the control of an OPERATOR REGION.

Top Publications

  1. ncbi Prokaryotic transcriptomics: a new view on regulation, physiology and pathogenicity
    Rotem Sorek
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
    Nat Rev Genet 11:9-16. 2010
  2. ncbi The primary transcriptome of the major human pathogen Helicobacter pylori
    Cynthia M Sharma
    Max Planck Institute for Infection Biology, RNA Biology Group, D 10117 Berlin, Germany
    Nature 464:250-5. 2010
  3. ncbi The complete genome sequence of Escherichia coli K-12
    F R Blattner
    Laboratory of Genetics, University of Wisconsin Madison, 445 Henry Mall, Madison, WI 53706, USA
    Science 277:1453-62. 1997
  4. pmc Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter
    L M Guzman
    Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Bacteriol 177:4121-30. 1995
  5. ncbi The Listeria transcriptional landscape from saprophytism to virulence
    Alejandro Toledo-Arana
    Institut Pasteur, Unité des Interactions Bactéries Cellules, F 75015 Paris, France
    Nature 459:950-6. 2009
  6. ncbi Transcriptome complexity in a genome-reduced bacterium
    Marc Güell
    Centre for Genomic Regulation CRG, Universitat Pompeu Fabra, Barcelona, Spain
    Science 326:1268-71. 2009
  7. pmc A single-base resolution map of an archaeal transcriptome
    Omri Wurtzel
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel
    Genome Res 20:133-41. 2010
  8. pmc An efficient recombination system for chromosome engineering in Escherichia coli
    D Yu
    Gene Regulation and Chromosome Biology Laboratory and Mouse Cancer Genetics Program, National Cancer Institute, Division of Basic Science, National Cancer Institute Frederick Cancer Research and Development Center, Frederick, MD 21702, USA
    Proc Natl Acad Sci U S A 97:5978-83. 2000
  9. ncbi Prokaryotic toxin-antitoxin stress response loci
    Kenn Gerdes
    Department of Biochemistry and Molecular Biology, University of Southern Denmark, DK 5230 Odense M, Denmark
    Nat Rev Microbiol 3:371-82. 2005
  10. pmc Comprehensive comparative-genomic analysis of type 2 toxin-antitoxin systems and related mobile stress response systems in prokaryotes
    Kira S Makarova
    National Center for Biotechnology Information, NLM, National Institutes of Health, Bethesda, Maryland 20894, USA
    Biol Direct 4:19. 2009

Detail Information

Publications310 found, 100 shown here

  1. ncbi Prokaryotic transcriptomics: a new view on regulation, physiology and pathogenicity
    Rotem Sorek
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
    Nat Rev Genet 11:9-16. 2010
    ..Deep RNA sequencing and tiling array studies are now revolutionizing our understanding of the complexity, plasticity and regulation of microbial transcriptomes...
  2. ncbi The primary transcriptome of the major human pathogen Helicobacter pylori
    Cynthia M Sharma
    Max Planck Institute for Infection Biology, RNA Biology Group, D 10117 Berlin, Germany
    Nature 464:250-5. 2010
    ..Our approach establishes a paradigm for mapping and annotating the primary transcriptomes of many living species...
  3. ncbi The complete genome sequence of Escherichia coli K-12
    F R Blattner
    Laboratory of Genetics, University of Wisconsin Madison, 445 Henry Mall, Madison, WI 53706, USA
    Science 277:1453-62. 1997
    ..The genome also contains insertion sequence (IS) elements, phage remnants, and many other patches of unusual composition indicating genome plasticity through horizontal transfer...
  4. pmc Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter
    L M Guzman
    Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Bacteriol 177:4121-30. 1995
    ..have constructed a series of plasmid vectors (pBAD vectors) containing the PBAD promoter of the araBAD (arabinose) operon and the gene encoding the positive and negative regulator of this promoter, araC...
  5. ncbi The Listeria transcriptional landscape from saprophytism to virulence
    Alejandro Toledo-Arana
    Institut Pasteur, Unité des Interactions Bactéries Cellules, F 75015 Paris, France
    Nature 459:950-6. 2009
    ..We provide the complete Listeria operon map and have uncovered far more diverse types of RNAs than expected: in addition to 50 small RNAs (<500 ..
  6. ncbi Transcriptome complexity in a genome-reduced bacterium
    Marc Güell
    Centre for Genomic Regulation CRG, Universitat Pompeu Fabra, Barcelona, Spain
    Science 326:1268-71. 2009
    ..Frequent antisense transcripts, alternative transcripts, and multiple regulators per gene imply a highly dynamic transcriptome, more similar to that of eukaryotes than previously thought...
  7. pmc A single-base resolution map of an archaeal transcriptome
    Omri Wurtzel
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel
    Genome Res 20:133-41. 2010
    ..of 625 million bases of sequenced cDNAs yielded a single-base-pair resolution map of transcription start sites and operon structures for more than 1000 transcriptional units...
  8. pmc An efficient recombination system for chromosome engineering in Escherichia coli
    D Yu
    Gene Regulation and Chromosome Biology Laboratory and Mouse Cancer Genetics Program, National Cancer Institute, Division of Basic Science, National Cancer Institute Frederick Cancer Research and Development Center, Frederick, MD 21702, USA
    Proc Natl Acad Sci U S A 97:5978-83. 2000
    ..This system will be especially useful for the engineering of large bacterial plasmids such as those from bacterial artificial chromosome libraries...
  9. ncbi Prokaryotic toxin-antitoxin stress response loci
    Kenn Gerdes
    Department of Biochemistry and Molecular Biology, University of Southern Denmark, DK 5230 Odense M, Denmark
    Nat Rev Microbiol 3:371-82. 2005
    ..It has been proposed that toxin-antitoxin loci function in bacterial programmed cell death, but evidence now indicates that these loci provide a control mechanism that helps free-living prokaryotes cope with nutritional stress...
  10. pmc Comprehensive comparative-genomic analysis of type 2 toxin-antitoxin systems and related mobile stress response systems in prokaryotes
    Kira S Makarova
    National Center for Biotechnology Information, NLM, National Institutes of Health, Bethesda, Maryland 20894, USA
    Biol Direct 4:19. 2009
    ....
  11. pmc DOOR: a database for prokaryotic operons
    Fenglou Mao
    Computational Systems Biology Laboratory, Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602, USA
    Nucleic Acids Res 37:D459-63. 2009
    ..All the operons in DOOR are predicted using our own prediction program, which was ranked to be the best among 14 operon prediction programs by a recent independent review...
  12. pmc STRING: a web-server to retrieve and display the repeatedly occurring neighbourhood of a gene
    B Snel
    European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    Nucleic Acids Res 28:3442-4. 2000
    ..The usefulness of STRING is illustrated with an example that suggests a functional context for an RNA methylase with unknown specificity...
  13. pmc DBTBS: a database of transcriptional regulation in Bacillus subtilis containing upstream intergenic conservation information
    Nicolas Sierro
    Human Genome Center, The Institute of Medical Science, University of Tokyo, 4 6 1 Shirokanedai, Minato ku, Tokyo, Japan
    Nucleic Acids Res 36:D93-6. 2008
    ..DBTBS can be accessed at: http://dbtbs.hgc.jp...
  14. ncbi A major protein component of the Bacillus subtilis biofilm matrix
    Steven S Branda
    Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115, USA
    Mol Microbiol 59:1229-38. 2006
    ..Having defined the major components of the biofilm matrix and the control of their synthesis by the global regulator SinR, we present a working model for how B. subtilis switches between nomadic and sedentary lifestyles...
  15. ncbi Determination of the regulon and identification of novel mRNA targets of Pseudomonas aeruginosa RsmA
    Anja Brencic
    Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115, USA
    Mol Microbiol 72:612-32. 2009
    ..Our work supports a model in which RsmA acts as a negative translational regulator, and where its positive effects are achieved indirectly by RsmA-mediated interference with translation of specific regulatory factors...
  16. pmc RegPrecise: a database of curated genomic inferences of transcriptional regulatory interactions in prokaryotes
    Pavel S Novichkov
    Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA, State Scientific Center GosNIIGenetika, Moscow 117545, Russia
    Nucleic Acids Res 38:D111-8. 2010
    ..Another section in the database represents the results of accurate regulon propagation to the closely related genomes...
  17. pmc ProOpDB: Prokaryotic Operon DataBase
    Blanca Taboada
    Centro de Ciencias Aplicadas y Desarrollo Tecnologico, Universidad Nacional Autonoma de Mexico, Mexico, DF, Mexico
    Nucleic Acids Res 40:D627-31. 2012
    The Prokaryotic Operon DataBase (ProOpDB, http://operons.ibt.unam.mx/OperonPredictor) constitutes one of the most precise and complete repositories of operon predictions now available...
  18. ncbi Global analysis of small RNA and mRNA targets of Hfq
    Aixia Zhang
    Cell Biology and Metabolism Branch, National Institute of Child Health and Development, Bethesda MD 20892, USA
    Mol Microbiol 50:1111-24. 2003
    ..Within the argX-hisR-leuT-proM operon, Hfq appears to compete with RNase E and modulate RNA processing and degradation...
  19. pmc Phosphoenolpyruvate:carbohydrate phosphotransferase systems of bacteria
    P W Postma
    E C Slater Institute, University of Amsterdam, The Netherlands
    Microbiol Rev 57:543-94. 1993
    ..In gram-positive bacteria, it has been demonstrated that HPr can be phosphorylated by ATP on a serine residue and this modification may perform a regulatory function...
  20. ncbi A master regulator for biofilm formation by Bacillus subtilis
    Daniel B Kearns
    Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Avenue, Cambridge, MA 02138, USA
    Mol Microbiol 55:739-49. 2005
    ..Critical to biofilm formation is the eps operon, which is believed to be responsible for the biosynthesis of an exopolysaccharide that binds chains of cells ..
  21. pmc Global RNA half-life analysis in Escherichia coli reveals positional patterns of transcript degradation
    Douglas W Selinger
    Harvard Medical School, Department of Genetics, Boston, Massachusetts 02115, USA
    Genome Res 13:216-23. 2003
    ..Unexpectedly, transcription of a single operon (tdcABCDEFG) was relatively rifampicin-insensitive and showed significant increases 2...
  22. pmc Functional analysis of genes for biosynthesis of pyocyanin and phenazine-1-carboxamide from Pseudomonas aeruginosa PAO1
    D V Mavrodi
    Department of Plant Pathology, Agricultural Research Service, Washington State University, Pullman, Washington 99164 6430, USA
    J Bacteriol 183:6454-65. 2001
    ..aeruginosa consisting of two core loci responsible for synthesis of PCA and three additional genes encoding unique enzymes involved in the conversion of PCA to pyocyanin, 1-hydroxyphenazine, and phenazine-1-carboxamide...
  23. pmc A high resolution map of a cyanobacterial transcriptome
    Vikram Vijayan
    Graduate Program in Systems Biology, Harvard University, 52 Oxford Street, Northwest 445, 40, Cambridge, MA 02138, USA
    Genome Biol 12:R47. 2011
    ....
  24. ncbi AraC protein, regulation of the l-arabinose operon in Escherichia coli, and the light switch mechanism of AraC action
    Robert Schleif
    Biology Department, Johns Hopkins University, 3400 N Charles St, Baltimore, MD 21218, USA
    FEMS Microbiol Rev 34:779-96. 2010
    This review covers the physiological aspects of regulation of the arabinose operon in Escherichia coli and the physical and regulatory properties of the operon's controlling gene, araC...
  25. pmc Effect of RyhB small RNA on global iron use in Escherichia coli
    Eric Massé
    Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA
    J Bacteriol 187:6962-71. 2005
    ..The two major operons involved in Fe-S cluster assembly showed different behavior; the isc operon appears to be a direct target of RyhB action, while the suf operon does not...
  26. pmc Molecular mechanisms of HipA-mediated multidrug tolerance and its neutralization by HipB
    Maria A Schumacher
    Department of Biochemistry and Molecular Biology, University of Texas, M D Anderson Cancer Center, Unit 1000, Houston, TX 77030, USA
    Science 323:396-401. 2009
    ..Dimeric HipB interacts with two HipA molecules to inhibit its kinase activity through sequestration and conformational inactivation. Combined, these studies suggest mechanisms for HipA-mediated persistence and its neutralization by HipB...
  27. ncbi Inactivation of the dlt operon in Staphylococcus aureus confers sensitivity to defensins, protegrins, and other antimicrobial peptides
    A Peschel
    Mikrobielle Genetik, Universitat Tubingen, Waldhäuser Strasse 70 8, 72076 Tubingen, Germany
    J Biol Chem 274:8405-10. 1999
    ..Wild-type strains bearing additional copies of the dlt operon produced teichoic acids with higher amounts of D-alanine esters, bound cationic proteins less effectively and were ..
  28. pmc Rv2686c-Rv2687c-Rv2688c, an ABC fluoroquinolone efflux pump in Mycobacterium tuberculosis
    Maria Rosalia Pasca
    Department of Genetics and Microbiology, University of Pavia, 27100 Pavia, Italy
    Antimicrob Agents Chemother 48:3175-8. 2004
    The Mycobacterium tuberculosis Rv2686c-Rv2687c-Rv2688c operon, encoding an ABC transporter, conferred resistance to ciprofloxacin and, to a lesser extent, norfloxacin, moxifloxacin, and sparfloxacin to Mycobacterium smegmatis...
  29. ncbi The recA gene of Streptococcus pneumoniae is part of a competence-induced operon and controls lysogenic induction
    B Martin
    Microbiologie et Génétique Moléculaire CNRS UPR, Universite Paul Sabatier, Toulouse, France
    Mol Microbiol 15:367-79. 1995
    ..7 kb long. This transcript indicated that recA is part of a competence-inducible (cin) operon. The major (about 4...
  30. pmc Structural and operational complexity of the Geobacter sulfurreducens genome
    Yu Qiu
    Department of Bioengineering, University of California, San Diego, La Jolla, California 92093, USA
    Genome Res 20:1304-11. 2010
    ..The experimentally determined structural and operational annotations can be combined with functional annotation, yielding a new three-level annotation that greatly expands our understanding of prokaryotic genomes...
  31. pmc The life-cycle of operons
    Morgan N Price
    Lawrence Berkeley National Laboratory, Berkeley, California, USA
    PLoS Genet 2:e96. 2006
    Operons are a major feature of all prokaryotic genomes, but how and why operon structures vary is not well understood...
  32. pmc Evolution of the chaperone/usher assembly pathway: fimbrial classification goes Greek
    Sean Paul Nuccio
    Department of Medical Microbiology and Immunology, School of Medicine, University of California at Davis, One Shields Ave, Davis, CA 95616 8645, USA
    Microbiol Mol Biol Rev 71:551-75. 2007
    ..Members of each clade share related operon structures and encode fimbrial subunits with similar protein domains...
  33. pmc A Bacillus subtilis sensor kinase involved in triggering biofilm formation on the roots of tomato plants
    Yun Chen
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    Mol Microbiol 85:418-30. 2012
    ..We propose that small signalling molecules released by the roots of tomato plants are directly or indirectly recognized by KinD, triggering biofilm formation...
  34. pmc Cell population heterogeneity during growth of Bacillus subtilis
    Daniel B Kearns
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    Genes Dev 19:3083-94. 2005
    ..SwrA stimulates the transcription of a large operon (the flagellum/chemotaxis operon), which includes the genes for sigma(D) and an activator of sigma(D)-directed ..
  35. pmc Bistability and biofilm formation in Bacillus subtilis
    Yunrong Chai
    Department of Molecular and Cellular Biology, The Biological Laboratories, Harvard University, Cambridge, MA 02138, USA
    Mol Microbiol 67:254-63. 2008
    ..The exopolysaccharide is produced by enzymes encoded by the epsA-O operon and the gene encoding TasA is located in the yqxM-sipW-tasA operon...
  36. ncbi Prokaryotic genome regulation: multifactor promoters, multitarget regulators and hierarchic networks
    Akira Ishihama
    Department of Frontier Bioscience, Hosei University, Koganei, Tokyo 184 8584, Japan
    FEMS Microbiol Rev 34:628-45. 2010
    ..The multifactor promoters and the multitarget transcription factors can be assembled into complex networks of transcription regulation, forming hierarchical networks...
  37. ncbi Positive regulation of motility and flhDC expression by the RNA-binding protein CsrA of Escherichia coli
    B L Wei
    Department of Molecular Biology and Immunology, University of North Texas Health Science Center at Fort Worth, 3500 Camp Bowie Blvd, Fort Worth, TX 76107 2699, USA
    Mol Microbiol 40:245-56. 2001
    ..regulated by several global regulatory circuits, which converge to modulate the overall expression of the master operon for flagellum biosynthesis, flhDC. We now show that the global regulator CsrA of E...
  38. ncbi Targets of the master regulator of biofilm formation in Bacillus subtilis
    Frances Chu
    Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Avenue, Cambridge, MA 02138, USA
    Mol Microbiol 59:1216-28. 2006
    ..The formation of these biofilms is mediated in part by the 15-gene exopolysaccharide operon, epsA-O, which is under the direct negative control of the SinR repressor...
  39. pmc The Brucella suis virB operon is induced intracellularly in macrophages
    Maria Laura Boschiroli
    Institut National de la Sante et de la Recherche Medicale, Unité 431, Faculte de Medecine, 30900 Nimes, France
    Proc Natl Acad Sci U S A 99:1544-9. 2002
    ..Reverse transcriptase-PCR showed that the 12 genes encoding the Brucella suis VirB system form an operon. Semiquantitative measurements of virB mRNA levels by slot blotting showed that transcription of the virB operon, ..
  40. ncbi Messenger RNA interferase RelE controls relBE transcription by conditional cooperativity
    Martin Overgaard
    Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK 5230 Odense M, Denmark
    Mol Microbiol 69:841-57. 2008
    Prokaryotic toxin-antitoxin (TA) loci consist of two genes in an operon that encodes a metabolically stable toxin and an unstable antitoxin. The antitoxin neutralizes its cognate toxin by forming a tight complex with it...
  41. ncbi Molecular insights into the control of transcription initiation at the Staphylococcus aureus agr operon
    Jonathan Reynolds
    Section of Microbiology, Faculty of Medicine and Centre for Molecular Microbiology and Infection, Imperial College London, London SW7 2AZ, UK
    J Mol Biol 412:862-81. 2011
    The accessory gene regulatory (agr) operon of the opportunistic human pathogen Staphylococcus aureus is a prime pathogenesis factor in this bacterium...
  42. pmc The FleQ protein from Pseudomonas aeruginosa functions as both a repressor and an activator to control gene expression from the pel operon promoter in response to c-di-GMP
    Claudine Baraquet
    Department of Microbiology, University of Washington, Seattle, WA 98195 7735, USA
    Nucleic Acids Res 40:7207-18. 2012
    ..The mechanism of action of FleQ is distinct from that of other bacterial transcription factors that both activate and repress gene expression from a single promoter...
  43. ncbi The emb operon, a gene cluster of Mycobacterium tuberculosis involved in resistance to ethambutol
    A Telenti
    Institute for Medical Microbiology, University of Bern, Switzerland
    Nat Med 3:567-70. 1997
    ..Further characterization of these proteins might provide information on targets for new chemotherapeutic agents and might help development of diagnostic strategies for the detection of resistant M. tuberculosis...
  44. pmc CmeABC functions as a multidrug efflux system in Campylobacter jejuni
    Jun Lin
    Food Animal Health Research Program, Department of Veterinary Preventive Medicine, The Ohio State University, Wooster, Ohio 44691, USA
    Antimicrob Agents Chemother 46:2124-31. 2002
    ..Here we characterized an efflux pump encoded by a three-gene operon (designated cmeABC) that contributes to multidrug resistance in C. jejuni 81-176...
  45. pmc Regulation of Pseudomonas quinolone signal synthesis in Pseudomonas aeruginosa
    Dana S Wade
    Department of Microbiology and Immunology, East Carolina University School of Medicine, BT 132, 600 Moye Blvd, Greenville, NC 27834, USA
    J Bacteriol 187:4372-80. 2005
    ..Previous experiments showed that the transcription of this operon, and therefore PQS production, is negatively regulated by the rhl quorum-sensing system and positively regulated ..
  46. ncbi Ordering genes in a flagella pathway by analysis of expression kinetics from living bacteria
    S Kalir
    Department of Molecular Cell Biology, Department of Physics of Complex Systems, Weizmann Institute of Science, Rehovot 76100, Israel
    Science 292:2080-3. 2001
    ..The observed temporal program of transcription was much more detailed than was previously thought and was associated with multiple steps of flagella assembly...
  47. ncbi Combinatorial engineering of intergenic regions in operons tunes expression of multiple genes
    Brian F Pfleger
    Department of Chemical Engineering, University of California Berkeley, Berkeley, CA 94720 1462, USA
    Nat Biotechnol 24:1027-32. 2006
    ..the relative expression of two reporter genes over a 100-fold range and balance expression of three genes in an operon that encodes a heterologous mevalonate biosynthetic pathway, resulting in a sevenfold increase in mevalonate ..
  48. pmc The specialized secretory apparatus ESX-1 is essential for DNA transfer in Mycobacterium smegmatis
    Abbie Coros
    The Microscopy and Imaging Center, Texas A and M University, 2257 TAMU, College Station, TX 77843, USA
    Mol Microbiol 69:794-808. 2008
    ..smegmatis DNA transfer and M. tuberculosis virulence...
  49. pmc Bacterial toxin-antitoxin systems: more than selfish entities?
    Laurence Van Melderen
    Laboratoire de Génétique et Physiologie Bactérienne, IBMM, Faculte des Sciences, Universite Libre de Bruxelles, Gosselies, Belgium
    PLoS Genet 5:e1000437. 2009
    ..This Review discusses current hypotheses regarding the biological roles of these evolutionarily successful small operons. We consider the various selective forces that could drive the maintenance of TA systems in bacterial genomes...
  50. pmc Multiple control of flagellum biosynthesis in Escherichia coli: role of H-NS protein and the cyclic AMP-catabolite activator protein complex in transcription of the flhDC master operon
    O Soutourina
    Unité de Régulation de l Expression Génétique, Institut Pasteur, 75724 Paris Cedex 15, France
    J Bacteriol 181:7500-8. 1999
    ..Overexpression of the flhDC master operon restored, at least in part, motility in crp and hns mutant strains, suggesting that this operon is the main target ..
  51. ncbi Processing of a sporulation sigma factor in Bacillus subtilis: how morphological structure could control gene expression
    P Stragier
    Institut de Microbiologie, Universite Paris Sud, Orsay, France
    Cell 52:697-704. 1988
    ..According to this model, a morphological structure would directly control the synthesis of a developmental sigma factor and would modify gene expression...
  52. ncbi Group A streptococcal growth phase-associated virulence factor regulation by a novel operon (Fas) with homologies to two-component-type regulators requires a small RNA molecule
    B Kreikemeyer
    Department of Medical Microbiology and Hygiene, University Hospital Ulm, Robert-Koch-Str. 8, D-89081 Ulm, Germany
    Mol Microbiol 39:392-406. 2001
    ..The fas operon, present in all 12 tested M serotypes, was transcribed as polycystronic message (fasBCA) and contained genes ..
  53. pmc Mechanisms causing rapid and parallel losses of ribose catabolism in evolving populations of Escherichia coli B
    V S Cooper
    Center for Microbial Ecology, Michigan State University, East Lansing, Michigan 48824, USA
    J Bacteriol 183:2834-41. 2001
    ..At the molecular level, the loss of ribose catabolic function involved the deletion of part or all of the ribose operon (rbs genes)...
  54. pmc Salmonella enterica serovar Typhi possesses a unique repertoire of fimbrial gene sequences
    S M Townsend
    Department of Medical Microbiology and Immunology, College of Medicine, Texas A and M University, College Station, Texas 77843, USA
    Infect Immun 69:2894-901. 2001
    ..genome, which has been sequenced by the Typhi Sequencing Group at the Sanger Centre, contained a type IV fimbrial operon, an orthologue of the agf operon, and 12 putative fimbrial operons of the chaperone-usher assembly class...
  55. pmc Identification and characterization of a Streptococcus pyogenes operon involved in binding of hemoproteins and acquisition of iron
    Christopher S Bates
    Department of Biology, College of Arts and Sciences, Georgia State University, Atlanta, Georgia 30303, USA
    Infect Immun 71:1042-55. 2003
    ..The sia transporter is part of a highly conserved, iron regulated, 10-gene operon. SiaA, which was localized to the cell membrane, could specifically bind hemoglobin...
  56. pmc Curli fibers are highly conserved between Salmonella typhimurium and Escherichia coli with respect to operon structure and regulation
    U Romling
    Department of Bacteriology, Microbiology and Tumorbiology Center, Karolinska Institutet, Stockholm, Sweden
    J Bacteriol 180:722-31. 1998
    ..The high degree of conservation at the protein level and the identical regulation patterns in E. coli and S. typhimurium suggest similar roles of curli fibers in the same ecological niche in the two species...
  57. ncbi Glutamate decarboxylase-dependent acid resistance in orally acquired bacteria: function, distribution and biomedical implications of the gadBC operon
    Daniela De Biase
    Istituto Pasteur Fondazione Cenci Bolognetti, Dipartimento di Scienze e Biotecnologie Medico Chirurgiche, Sapienza Universita di Roma, 04100, Latina, Italy
    Mol Microbiol 86:770-86. 2012
    ....
  58. pmc A predicted operon map for Mycobacterium tuberculosis
    P Roback
    Department of Mathematics, Statistics and Computer Science, St Olaf College, Northfield, MN 55057, USA
    Nucleic Acids Res 35:5085-95. 2007
    ..From this model, we rank each potential operon pair by the strength of evidence for cotranscription, choose a classification threshold with a true positive rate ..
  59. pmc Gene position in a long operon governs motility development in Bacillus subtilis
    Loralyn M Cozy
    Department of Biology, Indiana University, Bloomington, IN 47405, USA
    Mol Microbiol 76:273-85. 2010
    ..The proportion of ON cells increased when sigD was artificially moved forward in the 27 kb fla/che operon. In addition, we identified a new sigma(D)-dependent promoter that increases sigD expression and may provide ..
  60. ncbi Glycogen in Bacillus subtilis: molecular characterization of an operon encoding enzymes involved in glycogen biosynthesis and degradation
    J A Kiel
    Department of Genetics, State University of Groningen, Haren, The Netherlands
    Mol Microbiol 11:203-18. 1994
    ..subtilis containing a glycogen operon. The operon is located directly downstream from trnB, which maps at 275 degrees on the B. subtilis chromosome...
  61. pmc Transcriptional regulation of the Clostridium cellulolyticum cip-cel operon: a complex mechanism involving a catabolite-responsive element
    Laetitia Abdou
    Laboratoire de Bioenergetique et Ingenierie des Proteines, UPR 9036 CNRS, Universite d Aix Marseille, Marseille, France
    J Bacteriol 190:1499-506. 2008
    ..414 nucleotides downstream from the transcriptional start site has been shown to be involved in regulating this operon by a carbon catabolite repression mechanism...
  62. pmc Regulation of Corynebacterium glutamicum heat shock response by the extracytoplasmic-function sigma factor SigH and transcriptional regulators HspR and HrcA
    Shigeki Ehira
    Research Institute of Innovative Technology for the Earth, 9 2 Kizugawadai, Kizugawa, Kyoto 619 0292, Japan
    J Bacteriol 191:2964-72. 2009
    ..glutamicum heat shock response. Upregulation of 18 genes encoding transcriptional regulators by heat shock suggests a complex regulatory network of heat shock response in C. glutamicum...
  63. pmc Activation of the Pseudomonas aeruginosa type III secretion system requires an intact pyruvate dehydrogenase aceAB operon
    Denis Dacheux
    Departement de Biologie Moleculaire et Structurale, BBSI, UMR 5092 CNRS, CEA Grenoble, CHU Grenoble, Grenoble, France
    Infect Immun 70:3973-7. 2002
    ..Among 25 mutants affected in TTSS function, 14 contained the insertion at different positions in the aceAB operon encoding the PDH-E1 and -E2 subunits of pyruvate dehydrogenase...
  64. ncbi Probing the ArcA-P modulon of Escherichia coli by whole genome transcriptional analysis and sequence recognition profiling
    Xueqiao Liu
    Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115, USA
    J Biol Chem 279:12588-97. 2004
    ..However, the discovery of 3 targets that are not implicated in respiratory metabolism (the tra operon for plasmid conjugation, psi site for Xer-based recombination, and oriC site for chromosome replication) suggests ..
  65. ncbi Ferric uptake regulation protein acts as a repressor, employing iron (II) as a cofactor to bind the operator of an iron transport operon in Escherichia coli
    A Bagg
    Department of Biochemistry, University of California, Berkeley 94720
    Biochemistry 26:5471-7. 1987
    The Fur (ferric uptake regulation) protein is a negative regulator of the aerobactin operon and of several other siderophore-mediated, high-affinity iron transport systems in Escherichia coli...
  66. pmc Genomic arrangement of bacterial operons is constrained by biological pathways encoded in the genome
    Yanbin Yin
    Computational Systems Biology Laboratory, Department of Biochemistry and Molecular Biology and Institute of Bioinformatics, University of Georgia, Athens, GA 30605, USA
    Proc Natl Acad Sci U S A 107:6310-5. 2010
    ..little is understood about what may determine the global arrangement of bacterial genes in a genome beyond the operon level...
  67. pmc Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations
    Yutaka Satou
    Department of Zoology, Graduate School of Science, Kyoto University, Sakyo, Kyoto, Japan
    Genome Biol 9:R152. 2008
    ....
  68. ncbi Formation of D-alanyl-lipoteichoic acid is required for adhesion and virulence of Listeria monocytogenes
    Eric Abachin
    Laboratoire de Microbiologie, INSERM U 411, Paris, France
    Mol Microbiol 43:1-14. 2002
    The dlt operon of Gram-positive bacteria comprises four genes (dltA, dltB, dltC and dltD) that catalyse the incorporation of D-alanine residues into the cell wall-associated lipoteichoic acids (LTAs)...
  69. pmc Genetic organization and iron-responsive regulation of the Brucella abortus 2,3-dihydroxybenzoic acid biosynthesis operon, a cluster of genes required for wild-type virulence in pregnant cattle
    Bryan H Bellaire
    Department of Microbiology and Immunology, Louisiana State University Health Sciences Center, Shreveport, LA 71130 3932, USA
    Infect Immun 71:1794-803. 2003
    ..of total RNA with dhb-specific primers demonstrated that dhbC, -B, and -A are transcribed as components of an operon, together with dhbE, a functional homolog of the Escherichia coli entE gene. Homologs of the E...
  70. pmc The pio operon is essential for phototrophic Fe(II) oxidation in Rhodopseudomonas palustris TIE-1
    Yongqin Jiao
    Division of Geological and Planetary Sciences, California Institute of Technology, Pasadena, CA 91125, USA
    J Bacteriol 189:1765-73. 2007
    ..Here we report the discovery, with Rhodopseudomonas palustris TIE-1 as a model organism, of a three-gene operon, designated the pio operon (for phototrophic iron oxidation), that is necessary for phototrophic Fe(II) oxidation...
  71. pmc The σB-dependent yabJ-spoVG operon is involved in the regulation of extracellular nuclease, lipase, and protease expression in Staphylococcus aureus
    Bettina Schulthess
    Institute of Medical Microbiology, University of Zurich, Gloriastrasse 32, 8006 Zurich, Switzerland
    J Bacteriol 193:4954-62. 2011
    ..The σ(B)-controlled yabJ-spoVG operon encodes two such putative downstream elements. We report here transcriptome analysis in S...
  72. pmc Functional characterization of the sigmaB-dependent yabJ-spoVG operon in Staphylococcus aureus: role in methicillin and glycopeptide resistance
    Bettina Schulthess
    Institute of Medical Microbiology, University of Zurich, Gloriastr 32, 8006 Zurich, Switzerland
    Antimicrob Agents Chemother 53:1832-9. 2009
    ..aureus (GISA) strains. We show here that deletion of the sigma(B)-controlled yabJ-spoVG operon, which codes for potential downstream regulators of sigma(B), abolished capsule synthesis and reduced resistance ..
  73. pmc Description of staphylococcus serine protease (ssp) operon in Staphylococcus aureus and nonpolar inactivation of sspA-encoded serine protease
    K Rice
    University of Toronto Department of Laboratory Medicine and Pathobiology, and Sunnybrook and Womens College Health Science Centre, Department of Microbiology, North York, Ontario, Canada M4N 3M5
    Infect Immun 69:159-69. 2001
    ..In this study, we describe an operon structure within the ssp locus of S. aureus RN6390...
  74. ncbi 50 million years of genomic stasis in endosymbiotic bacteria
    Ivica Tamas
    Department of Molecular Evolution, Evolutionary Biology Center, University of Uppsala, Uppsala, Sweden
    Science 296:2376-9. 2002
    ..The genomic stasis of B. aphidicola, likely attributable to the loss of phages, repeated sequences, and recA, indicates that B. aphidicola is no longer a source of ecological innovation for its hosts...
  75. pmc Identification of an aggregative adhesion fimbria (AAF) type III-encoding operon in enteroaggregative Escherichia coli as a sensitive probe for detecting the AAF-encoding operon family
    Christine Bernier
    Unité de Pathogénie Bactérienne des Muqueuses, Groupe d Etude des Infections Diarrhéiques GEID, Institut Pasteur, 75724 Paris Cedex 15, France
    Infect Immun 70:4302-11. 2002
    ..We developed PCR assays specific for the agg-3 operon. In our collection, the frequency of AAF-III strains was similar (12%) to that of AAF-I strains (16%) but higher ..
  76. pmc Genome-wide polysome profiling reveals an inflammation-responsive posttranscriptional operon in gamma interferon-activated monocytes
    Keyur Vyas
    Center for Gene Regulation in Health and Disease and Department of Biology, Cleveland State University, Cleveland, Ohio 44115, USA
    Mol Cell Biol 29:458-70. 2009
    ..Our studies have identified a new inflammation-responsive posttranscriptional operon that can be regulated directly at the level of translation in IFN-gamma-activated monocytes...
  77. pmc Global gene expression profiling of Yersinia pestis replicating inside macrophages reveals the roles of a putative stress-induced operon in regulating type III secretion and intracellular cell division
    Hana S Fukuto
    Center for Infectious Diseases, Department of Molecular Genetics and Biology, Stony Brook University, Stony Brook, NY 11794 5120, USA
    Infect Immun 78:3700-15. 2010
    ..A putative stress-induced operon consisting of y2313, y2315, and y2316 (y2313-y2316), and a previously unidentified open reading frame, orfX, was ..
  78. ncbi The dhb operon of Bacillus subtilis encodes the biosynthetic template for the catecholic siderophore 2,3-dihydroxybenzoate-glycine-threonine trimeric ester bacillibactin
    J J May
    Department of Chemistry, Philipps Universitat Marburg, D 35032 Marburg, Germany
    J Biol Chem 276:7209-17. 2001
    ..Comparative mass spectrometric analysis of culture extracts from both the wild type and the dhbF mutant led to the identification of a mass peak at m/z 881 ([M-H](1-)) that corresponds to a cyclic trimeric ester of DHB-glycine-threonine...
  79. pmc Identification of psl, a locus encoding a potential exopolysaccharide that is essential for Pseudomonas aeruginosa PAO1 biofilm formation
    Kara D Jackson
    Department of Microbiology and Immunology, Wake Forest University School of Medicine, Medical Center Blvd, Winston Salem, NC 27157 1064, USA
    J Bacteriol 186:4466-75. 2004
    ..Understanding psl-encoded exopolysaccharide expression and protection in biofilms will provide insight into the pathogenesis of P. aeruginosa in cystic fibrosis and other infections involving biofilms...
  80. pmc Functional and comparative genomic analyses of an operon involved in fructooligosaccharide utilization by Lactobacillus acidophilus
    Rodolphe Barrangou
    Genomic Sciences Program and Southeast Dairy Foods Research Center, North Carolina State University, Raleigh, NC 27695, USA
    Proc Natl Acad Sci U S A 100:8957-62. 2003
    ..Transcriptional analysis of this operon demonstrated that gene expression was induced by sucrose and FOS but not by glucose or fructose, suggesting some ..
  81. ncbi Overexpression of the mexC-mexD-oprJ efflux operon in nfxB-type multidrug-resistant strains of Pseudomonas aeruginosa
    K Poole
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada
    Mol Microbiol 21:713-24. 1996
    ..sequencing of portions of these fragments revealed that oprJ was the terminal gene in a putative three-gene operon, mexC-mexD-oprJ...
  82. pmc mexEF-oprN multidrug efflux operon of Pseudomonas aeruginosa: regulation by the MexT activator in response to nitrosative stress and chloramphenicol
    Hossam Fetar
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada
    Antimicrob Agents Chemother 55:508-14. 2011
    A null mutation in the mexS gene of Pseudomonas aeruginosa yielded an increased level of expression of a 3-gene operon containing a gene, xenB, whose product is highly homologous to a xenobiotic reductase in Pseudomonas fluorescens shown ..
  83. pmc Transcriptional regulation of the cellobiose operon of Streptococcus mutans
    Lin Zeng
    Department of Oral Biology, University of Florida, Gainesville, 32610, USA
    J Bacteriol 191:2153-62. 2009
    ..the hydrolysis of cellulose, is shown to be regulated by a transcriptional regulator, CelR, which is encoded by an operon with a phospho-beta-glucosidase (CelA) and a cellobiose-specific sugar phosphotransferase system (PTS) permease (..
  84. pmc The Staphylococcus aureus lrgAB operon modulates murein hydrolase activity and penicillin tolerance
    K H Groicher
    Department of Microbiology, Molecular Biology and Biochemistry, University of Idaho, Moscow, Idaho 83844 3052, USA
    J Bacteriol 182:1794-801. 2000
    ..A LytSR-regulated dicistronic operon has also been identified and shown to encode two potential membrane-associated proteins, designated LrgA and LrgB, ..
  85. pmc Characterization of the ebp(fm) pilus-encoding operon of Enterococcus faecium and its role in biofilm formation and virulence in a murine model of urinary tract infection
    Jouko Sillanpaa
    Department of Internal Medicine, and Center for the Study of Emerging and Re emerging Pathogens, Division of Infectious Diseases, University of Texas Medical School at Houston, Houston, TX, USA
    Virulence 1:236-46. 2010
    ..predicted pilus-encoding gene clusters; we also demonstrated that one of these, ebpABC(fm), is transcribed as an operon, that its putative major pilus subunit, EbpC(fm) (also called pilB), is polymerized into high molecular weight ..
  86. pmc Physiological studies of tryptophan transport and tryptophanase operon induction in Escherichia coli
    C Yanofsky
    Department of Biological Sciences, Stanford University, California 94305 5020
    J Bacteriol 173:6009-17. 1991
    ..We have exploited the fact that the tryptophanase (tna) operon is induced by tryptophan to infer how tryptophan transport is influenced by the growth medium and by mutations ..
  87. pmc Short RNA half-lives in the slow-growing marine cyanobacterium Prochlorococcus
    Claudia Steglich
    Massachusetts Institute of Technology, Department of Civil and Environmental Engineering, Cambridge, MA 02139, USA
    Genome Biol 11:R54. 2010
    ..We investigated whole-genome RNA stability of Prochlorococcus, a relatively slow-growing marine cyanobacterium doubling approximately once a day, which is extremely abundant in the oceans...
  88. ncbi Identification of the maturation factor for dual oxidase. Evolution of an eukaryotic operon equivalent
    Helmut Grasberger
    Department of Medicine, University of Chicago, Illinois 60637, USA
    J Biol Chem 281:18269-72. 2006
    ..The identification of DUOXA genes has important implications for studies of the molecular mechanisms controlling DUOX expression and the molecular genetics of congenital hypothyroidism...
  89. pmc Multiple antibiotic resistance in Pseudomonas aeruginosa: evidence for involvement of an efflux operon
    K Poole
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada
    J Bacteriol 175:7363-72. 1993
    ..The gene encoding OprK (previously identified as ORFC) forms part of an operon composed of three genes (ORFABC) implicated in the secretion of the siderophore pyoverdine...
  90. pmc HCF152, an Arabidopsis RNA binding pentatricopeptide repeat protein involved in the processing of chloroplast psbB-psbT-psbH-petB-petD RNAs
    Karin Meierhoff
    Heinrich Heine Universitat, Institut für Entwicklungs und Molekularbiologie der Pflanzen, 40225 Dusseldorf, Germany
    Plant Cell 15:1480-95. 2003
    The psbB-psbT-psbH-petB-petD operon of higher plant chloroplasts is a heterogeneously composed transcriptional unit that undergoes complex RNA processing events until the mature oligocistronic RNAs are formed...
  91. pmc The fox operon from Rhodobacter strain SW2 promotes phototrophic Fe(II) oxidation in Rhodobacter capsulatus SB1003
    Laura R Croal
    California Institute of Technology, Pasadena, CA 91125, USA
    J Bacteriol 189:1774-82. 2007
    ..study, we circumvented this problem by taking a heterologous-complementation approach to identify a three-gene operon (the foxEYZ operon) from Rhodobacter sp...
  92. pmc OperonDB: a comprehensive database of predicted operons in microbial genomes
    Mihaela Pertea
    Center for Bioinformatics and Computational Biology, University of Maryland, College Park, MD 20742, USA
    Nucleic Acids Res 37:D479-82. 2009
    ..OperonDB, first released in 2001, is a database containing the results of a computational algorithm for locating operon structures in microbial genomes...
  93. pmc Unusual regulation of a leaderless operon involved in the catabolism of dimethylsulfoniopropionate in Rhodobacter sphaeroides
    Matthew J Sullivan
    School of Biological Sciences, University of East Anglia, Norwich Research Park, Norwich, United Kingdom
    PLoS ONE 6:e15972. 2011
    ..It does so by using a lyase encoded by dddL, the promoter-distal gene of a three-gene operon, acuR-acuI-dddL...
  94. pmc Role of the multidrug resistance regulator MarA in global regulation of the hdeAB acid resistance operon in Escherichia coli
    Cristian Ruiz
    Center for Adaptation Genetics and Drug Resistance, Tufts University School of Medicine, 136 Harrison Ave, Boston, MA 02111, USA
    J Bacteriol 190:1290-7. 2008
    ..5. These findings show that repression of hdeAB by MarA depends on pH, growth phase, and other regulators of hdeAB and is associated with reduced resistance to acid conditions...
  95. pmc Noncognate Mycobacterium tuberculosis toxin-antitoxins can physically and functionally interact
    Ling Zhu
    Department of Biochemistry, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854 5635, USA
    J Biol Chem 285:39732-8. 2010
    ..As is characteristic of all toxin-antitoxin systems, each of the mazF-mt toxin genes is organized in an operon downstream of putative antitoxin genes...
  96. ncbi An oxygen-sensing diguanylate cyclase and phosphodiesterase couple for c-di-GMP control
    Jason R Tuckerman
    Department of Biochemistry, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, Texas 75390 9038, USA
    Biochemistry 48:9764-74. 2009
    ..Here we describe an Escherichia coli two-gene operon, dosCP, for control of c-di-GMP by oxygen...
  97. pmc Binding of Pseudomonas aeruginosa AlgZ to sites upstream of the algZ promoter leads to repression of transcription
    Deborah M Ramsey
    Department of Microbiology and Immunology, Wake Forest University School of Medicine, Medical Center Blvd, Winston Salem, NC 27157, USA
    J Bacteriol 187:4430-43. 2005
    ..The genes encoding the alginate biosynthetic enzymes are clustered in a single operon, which is under tight transcriptional control...
  98. pmc Role of the Pseudomonas aeruginosa oxyR-recG operon in oxidative stress defense and DNA repair: OxyR-dependent regulation of katB-ankB, ahpB, and ahpC-ahpF
    U A Ochsner
    Department of Microbiology, University of Colorado Health Sciences Center, Denver, Colorado 80262, USA
    J Bacteriol 182:4533-44. 2000
    ..and recG-lacZ reporter activities and oxyR-recG mRNA analysis showed that oxyR and recG are organized in an operon and expressed constitutively with regard to oxidative stress from a single promoter upstream of oxyR...
  99. pmc The methanogen-specific transcription factor MsvR regulates the fpaA-rlp-rub oxidative stress operon adjacent to msvR in Methanothermobacter thermautotrophicus
    Elizabeth A Karr
    Department of Botany and Microbiology, University of Oklahoma, Norman, OK 73019, USA
    J Bacteriol 192:5914-22. 2010
    ..assays were used to demonstrate that MsvR regulates transcription of the divergently transcribed fpaA-rlp-rub operon in Methanothermobacter thermautotrophicus in addition to transcription from its own promoter...
  100. pmc Identification of a reactivating factor for adenosylcobalamin-dependent ethanolamine ammonia lyase
    Koichi Mori
    Department of Bioscience and Biotechnology, Faculty of Engineering, Okayama University, Tsushima Naka, Okayama 700 8530, Japan
    J Bacteriol 186:6845-54. 2004
    ..The reactivation was observed in the permeabilized E. coli cells carrying a plasmid containing the E. coli eut operon as well...
  101. pmc Assessment of three Resistance-Nodulation-Cell Division drug efflux transporters of Burkholderia cenocepacia in intrinsic antibiotic resistance
    Silvia Buroni
    Department of Genetics and Microbiology, University of Pavia, Pavia, Italy
    BMC Microbiol 9:200. 2009
    ..cenocepacia J2315, but the contribution of these pumps to the intrinsic drug resistance of this bacterium remains unclear...

Research Grants72

  1. Group A Streptococcus responses to oxidative stress
    Ioannis Gryllos; Fiscal Year: 2010
    ..Our data also show that perR is co-transcribed from an operon encompassing four additional genes of which three are predicted to be involved in GAS stress responses...
  2. Role of the Mammalian Siderophore in Cellular Iron Metabolism and Erythropoiesis
    Laxminarayana R Devireddy; Fiscal Year: 2012
    ..These genes are highly homologous to a group of genes that constitute an operon controlling E. coli enterobactin synthesis...
  3. Characterization of new toxins (YmgD and YdfD) from E.coli, targeting cell wall
    Hisako Masuda; Fiscal Year: 2012
    ..The sets of these toxin and antitoxin pairs are most often encoded from the single operon, and termed TA systems...
  4. DlaT as a transcriptional regulator in non-replicating Mycobacterium tuberculosis
    Ruslana Bryk; Fiscal Year: 2012
    ..for Mtb's full virulence in mice and survival in guinea pigs;dlaT's chromosomal location outside the PDH operon suggests a function unrelated to coordinate expression of other PDH components;expression of DlaT increases in ..
  5. Rho-Modifying Cytotoxic Necrotizing Factor of E. coli
    ALISON DAVIS O'BRIEN; Fiscal Year: 2013
    ..e., cystitis or pyelonephritis, or acute prostatitis. In fact, the cnf1 locus and a hly operon are co-transcribed and co-regulated from a prototypic uropathogenic E. coli (UPEC) strain during culture in vitro...
  6. Quorum sensing regulation of EHEC virulence genes
    Vanessa Sperandio; Fiscal Year: 2013
    ..Finally QseBC activate expression of the qseEGF operon that encodes the histine sensor kinase QseE which also senses epinephrine;its cognate RR QseF (also phosphorylated ..
  7. Defining the functions of uncharacterized genes in priority pathogens
    Sean Crosson; Fiscal Year: 2013
    ..S. Centers for Disease Control (CDC). Experiments proposed herein will define the biochemical function of uncharacterized genes implicated in regulation of B. abortus stress physiology and in the control of chronic brucellosis disease. ..
  8. Reciprocal Control of Motility and Adherence in UTI
    Harry L Mobley; Fiscal Year: 2013
    ..specific aims: 1) Elucidate the prevalence, function, structure, and contribution to virulence of fimbrial operon-encoded repressors of motility: PapX and MrpJ;and 2) Define the regulatory pathways for proteins that mediate ..
  9. Mechanism of PsrP mediated adhesion
    Carlos J Orihuela; Fiscal Year: 2013
    ..We will: Aim 1. Determine the operon structure and transcriptional regulation of psrP-secY2A2...
  10. The Biosynthesis of Anandamide
    Dale G Deutsch; Fiscal Year: 2010
    ..These mice will also be transgenic for a Cre recombinase gene that is under the control of a tetracycline operon that can be turned on in the absence of doxycycline...
  11. Regulatory Mechanisms Controlling Expression of P. gingivalis Surface Structures
    MARY ELLEN DAVEY; Fiscal Year: 2013
    ..with high similarity to the DNA binding protein HU (PG0121) is transcribed with the K- antigen capsule synthesis operon and that this "histone-like" regulatory protein controls expression of the capsule operon...
  12. GENETIC STUDIES OF GLUTAMINE SYNTHETASE IN BACTERIA
    SYDNEY GOVONS KUSTU; Fiscal Year: 2010
    ..Our second long-term goal is to continue studies of the b1012 operon. This operon of 7 genes encodes a previously undescribed pathway for degradation of pyrimidine rings, which we ..
  13. Aminoglycoside potentiators for P. aeruginosa therapy
    Donald T Moir; Fiscal Year: 2013
    ..aeruginosa gene PA2549 promoter to a luciferase operon, and exhibits increased signal in response to deletion of ftsH and decreased signal in response to deletion of ..
  14. Structure-Function of the Shigella dysenteriae heme uptake operon (shu)
    Angela Wilks; Fiscal Year: 2012
    ..Heme utilization by S. dysenteriae is required for virulence and characterization of heme uptake and metabolism may provide novel therapeutic targets for drug development. ..
  15. Genetic control of denitrification in Pseudomonas aeruginosa
    Valley J Stewart; Fiscal Year: 2010
    ..In previous studies by others, the arcD operon encoding arginine fermentation enzymes has been suggested to be regulated exclusively by Anr, whereas the nosR ..
  16. Bacteriophage Control of DNA Repair in Streptococcus Pyogenes
    WILLIAM MICHAEL MCSHAN; Fiscal Year: 2013
    ..As we have shown in our previous studies, SpyCIM1 regulates the DNA mismatch repair (MMR) operon in M1 genome strain SF370 by altering between integrated and episomal forms in response to environmental signals...
  17. Novel components of iron sulfur cluster biosynthesis pathways
    Gareth P Butland; Fiscal Year: 2013
    ..are highly toxic to cells and FeS clusters are formed by specific biosynthesis pathways such as the iscRSUhscBAfdx operon and sufABCDSE operon in Escherichia coli...
  18. RmpA, a unique virulence regulator in emerging Klebsiella pneumoniae
    Valley J Stewart; Fiscal Year: 2012
    ..those involved directly in capsule synthesis and assembly;(3a) RcsB-RmpA heterodimers control capsule biosynthetic operon expression;and (3b) RmpA activity is modulated by phosphorylation...
  19. Genetic and Biochemical Analysis of DNA Exchange in Helicobacter pylori
    MARION SHONN DORER; Fiscal Year: 2010
    ..localization studies to understand the Lys mechanism of action as well as genetic study of two genes that form an operon with lys...
  20. Mechanisms of Action of UmuD in Regulating DNA Damage-Induced Genes in a umuC Def
    Janelle M Hare; Fiscal Year: 2013
    ..Unlike other bacteria, non-enteric Acinetobacter baylyi possesses an operon consisting of a umuD homolog, umuDAb, and a truncated, incomplete umuC...
  21. Pathogenic Mechanism for Lung Infection in Mucoid Pseudomonas
    DENNIS EDWARD OHMAN; Fiscal Year: 2013
    ..Most of the enzymes for the production of alginate are clustered in an operon of 12 genes...
  22. V. cholerae proteins are required for biofilm growth-vaccine targets
    HAVVA FITNAT YILDIZ; Fiscal Year: 2011
    ..Wade's laboratories has shown that anti-TcpA antibodies can modify biofilm growth. Other work by Dr. Yildiz's group has identified proteins required for biofilm development that will serve as immunogens to induce antibodies. ..
  23. Structural and functional studies of autoinducer-2 processing proteins
    Stephen T Miller; Fiscal Year: 2012
    ..A variety of bacterial species, including Escherichia coli, B. anthracis, and S. typhimurium have the lsr operon, a set of genes responsible for recognizing, internalizing, and processing AI-2 [20, 21]...
  24. Characterization of a New Key Regulator for Phenazine Production in Pseudomonads
    Christopher T Nomura; Fiscal Year: 2013
    ..aeruginosa. Ultimately, such knowledge has the potential to identify new targets to interfere with virulence factor production in P. aeruginosa. ..
  25. Regulation and Function of a Bacterial Cytoskeleton
    R Dyche Mullins; Fiscal Year: 2013
    ..The ParM gene is part of a partitioning locus (the par operon) found on many low-copy plasmids (e.g. clinically important R1 and R100 drug-resistance plasmids)...
  26. Mechanism of Pseudomonas-mediated epithelial cell damage
    Joanne N Engel; Fiscal Year: 2010
    ..We have investigated the role of the Chp operon and FimL in regulating twitching motility and T3SS...
  27. Impact of Diet on Intestinal Microbiota-Host Dynamics
    Justin Sonnenburg; Fiscal Year: 2013
    ..The aims of this proposal are to (i) gain mechanistic insight into the function of an operon conserved in Bacteroides required for use of abundant dietary plant polysaccharides called fructans;(ii) determine ..
  28. Mechanisms of Mycoplasmal Disease Pathogenesis
    KEVIN F DYBVIG; Fiscal Year: 2013
    ..These genes code for proteins that are annotated as ABC transporters. However, EPS-I is synthesized when an operon containing two of these genes are cloned into other species of mycoplasma...
  29. Studies of metal-dependent intercellular adhesion in Staphylococcal biofilms
    Andrew B Herr; Fiscal Year: 2013
    ..of Aap in the formation of cultured staphylococcal biofilms, and compare its function with that of the icaADBC operon responsible for biosynthesis of the biofilm polysaccharide...
  30. HuR in Allergic Asthma
    Ulus Atasoy; Fiscal Year: 2012
    ..We have developed a new paradigm, the posttranscriptional operon hypothesis, which states that RNA binding proteins are coordinately regulating the expression of biologically ..
  31. Genetic Mechanisms in Borrelia burgdorferi Pathogenesis
    Jonathan T Skare; Fiscal Year: 2013
    ..Recently, we found that two genes linked to bosR, bb0646 and bb0648, share a transcript and thus comprise an operon. These genes encode for an exported lipase (bb0646) and a serine/threonine kinase (bb0648), respectively, which we ..
  32. The RpoN-RpoS regulatory pathway in Borrelia burgdorferi
    Michael V Norgard; Fiscal Year: 2013
    ..the mechanism by which the RpoN-RpoS pathway regulates atypical expression of the decorin-binding protein (DbpB/A) operon. The dbpBA operon is under the control of the RpoN-RpoS pathway, but it has an expression pattern different from ..
  33. Mechanism of Folate Deficiency as a Co-Factor for HPV16-induced Carcinogenesis
    ASOK ANTONY; Fiscal Year: 2013
    ..hnRNP-E1) during physiological folate deficiency, activates a nutrition-sensitive posttranscriptional RNA operon that also includes an important interaction with two loci in Human Papillomavirus type-16 (HPV16) RNA...
  34. Apolipoprotein B and Control of S. aureus Quorum Sensing
    PAMELA RANEL HALL; Fiscal Year: 2013
    ..made in elucidating the MRSA virulence factors that contribute to infection and their control by a quorum sensing operon, agr, very little is known about host factors that contribute to susceptibility. Typically, S...
  35. Structural Biology of Purine and Pyrimidine Biosynthesis and Metabolism
    Steven E Ealick; Fiscal Year: 2012
    ..The purine catabolic operon encodes two novel enzymatic activities - a putative flavin- dependent uricase and an iron-dependent xanthine ..
  36. Intracellular Invasion by Streptococcus mutans: Significance in Disease
    JOSE A LEMOS; Fiscal Year: 2013
    ..Molecular characterization of the cnm locus), we will (i) conduct the transcriptional characterization of the cnm operon, (ii) employ mutagenesis approaches to continue the characterization of the Cnm and CsbB, and (iii) identify the ..
  37. Targeting Staphylococcus aureus Virulence
    Hattie D Gresham; Fiscal Year: 2013
    ..identified to date as essential for invasive MRSA infection are globally regulated in part by a quorum sensing operon, agr Importantly, our recent work identifying apolipoprotein B as an innate barrier that antagonizes agr signaling ..
  38. Genetic Requirements for the survival of Tubercle Bacilli in Nonhuman Primates
    Deepak Kaushal; Fiscal Year: 2013
    ....
  39. Trans-splicing in C. elegans
    Thomas Blumenthal; Fiscal Year: 2011
    ..These two events occur in close proximity between operon genes. Although 3'end formation is generally accompanied by transcription termination, in operons it is not...
  40. Regulation of transcription elongation
    PAUL L BABITZKE; Fiscal Year: 2013
    ..We developed the B. subtilis trp operon leader as a model system for RNA polymerase (RNAP) pausing and intrinsic termination...
  41. GONOCOCCAL INFECTION AND GENE EXPRESSION IN FEMALE MICE
    Ann E Jerse; Fiscal Year: 2013
    ..factors of the host innate defense, is critical for murine infection, and mutants that over-express the mtrCDE operon due to mutations in the mtrR repressor locus are more fit than wild type Gc in vivo...
  42. Contribution of Novel Frimbriae to Proteus Mirabilis Virulence
    Melanie M Pearson; Fiscal Year: 2013
    ..The majority of these regulators, identified by their homology to mrpJ in the mrp fimbrial operon, inhibit flagellar motility...
  43. The molecular control of bacterial autolysis
    Kenneth W Bayles; Fiscal Year: 2012
    ..The results generated by these studies will provide a better understanding of the molecular mechanisms controlling bacterial cell death and will lead to novel and more effective therapeutic strategies to combat infections. ..
  44. Borrelia burgdorferi tick phase genes and Lyme disease pathogenesis
    IRA S SCHWARTZ; Fiscal Year: 2013
    ..tick-phase protein with a unique expression profile during spirochete colonization of the vector;2) the Glp operon that encodes proteins required for glycerol uptake and utilization, a process likely to be particularly important ..
  45. Crystallization of outer membrane proteins for export of polysaccharide capsule
    Mark A Saper; Fiscal Year: 2012
    ..function in this role? Enteropathogenic and enterohaemorrhagic Escherichia coli require the seven genes of the gfc operon to produce a group 4 capsule comprised of O-antigen repeating units...
  46. Translational Silencing in Monocytes: Role of L13a
    Barsanjit Mazumder; Fiscal Year: 2013
    ..following three specific aims: (1) Analysis of the L13a regulated and inflammation responsive post-transcriptional operon in IFN-3 activated monocytes...
  47. PATHOGENIC FIBER FORMATION IN BACTERIA: STRUCTURAL BASIS
    Scott J Hultgren; Fiscal Year: 2013
    ....
  48. Transcriptional regulation of lipid homeostasis in mycobacteria
    Hugo Gramajo; Fiscal Year: 2013
    ..genetic analysis of conditional mutant strains in fas and mabR (the transcriptional regulator of the main fasII operon), both in Mycobacterium smegmatis and M...
  49. New mechanisms of sulfur sensing and trafficking in Staphylococcus aureus.
    David P Giedroc; Fiscal Year: 2013
    ..We have discovered a paralog of a copper-sensing transcriptional repressor CsoR (copper-sensitive operon repressor) in S. aureus strain Newman that we denote CstR, for CsoR-like sulfurtransferase repressor...
  50. Evaluation of Protein Channel-Attenuated Salmonella Vaccines
    Xinghong Yang; Fiscal Year: 2010
    ..Specific Aim 2: Determine which of the deletion mutants of the cfa/I operon attenuates wild-type Salmonella and can therefore be used as a protective vaccine against salmonellosis...
  51. Posttranscriptional Gene Regulation in Asthma
    Ulus Atasoy; Fiscal Year: 2010
    ..We have developed a new paradigm, the posttranscriptional operon hypothesis, which states that RNA binding proteins are coordinately regulating the expression of biologically ..
  52. cAMP signaling pathways within Mycobacterium tuberculosis
    Kathleen A McDonough; Fiscal Year: 2013
    ..3 addresses the roles of CrpMt and Cmr as co-regulators with other transcription factors, of the espA virulence operon using a combination of molecular, genetic and cellular approaches...
  53. Mechanisms of Resistance in Gram-Negative Bacteria
    Marcelo Carlos Sousa; Fiscal Year: 2010
    ..The enzymes responsible for the biosynthesis of 4- aminoarabinose-lipid A are clustered in an operon recently renamed to ArnBCADTEF...
  54. Environmental Adaptation and Membrane Physiology of an Oral Pathogen
    KEITH PETER MINTZ; Fiscal Year: 2013
    ..In Aggregatibacter and related genera, the gene is located in a three-gene operon under the control of a promoter responsive to environmental changes...
  55. Characterization of the ferrous iron transporter Feo in Vibrio cholerae
    Shelley M Payne; Fiscal Year: 2013
    ..Third, we will define the mechanism of regulation of expression of the feo operon and determine its pattern of expression when the bacteria are within the host...
  56. Structure-function relationship between multiple translation initiation factor 3
    APRIL DAWN NESBIT; Fiscal Year: 2012
    ..Furthermore, IF3a regulates the abundance of transcripts from the cpeC operon at the post-transcriptional level in response to changes in ambient light color...
  57. Effects of the nucleoid protein H-NS on RNAP elongation
    Matthew V Kotlajich; Fiscal Year: 2013
    ..Recent work from our lab found that the cryptic bgl operon has an antisense transcript that originates from the bglF gene, and transcription that initiates from the ..
  58. The RpoN-RpoS regulatory pathway in Borrelia burgdorferi
    MICHAEL NORGARD; Fiscal Year: 2009
    ..the mechanism by which the RpoN-RpoS pathway regulates atypical expression of the decorin-binding protein (DbpB/A) operon. The dbpBA operon is under the control of the RpoN-RpoS pathway, but it has an expression pattern different from ..
  59. Regulation of ESX-1 secretion and its role in M. tuberculosis virulence
    Jeffery S Cox; Fiscal Year: 2013
    ..EspR activates transcription of an operon that includes three ESX-1 components, whose expression in turn promotes secretion of ESX-1 substrates...
  60. Francisella tularensis Pathogenesis
    Thomas H Kawula; Fiscal Year: 2010
    ..with a transcriptional regulator, IclR, and IclR regulates a restricted number of genes, most notably an operon containing a response regulator, pmrA, that is required for F. tularensis intracellular growth and pathogenesis...
  61. ACTIVATION OF ALGINATE GENES IN PSEUDOMONAS AERUGINOSA
    DENNIS EDWARD OHMAN; Fiscal Year: 2013
    ..activate alginate biosynthesis by using sigma-54, which suggests an alternate pathway for expression of the algD operon for alginate biosynthesis...
  62. The Molecular Control of Cell Death in Staphylococcus aureus
    JEFFREY LEE BOSE; Fiscal Year: 2010
    ..The results generated by these studies will provide a better understanding of the molecular mechanisms controlling bacterial cell death and will lead to novel and more effective therapeutic strategies to combat infections. ..
  63. REGULATION OF PYRIMIDINE GENE EXPRESSION IN BACTERIA
    CHARLES TURNBOUGH; Fiscal Year: 2001
    ..reiterative transcription occurs at these promoters and is used for purine (ATP and/or GTP)-mediated regulation of operon expression...
  64. Pathogenesis of Persistent Chlamydial STDs
    Deborah Dean; Fiscal Year: 2007
    ..Further, CT contains a partial tryptophan biosynthesis operon (trpR, trpA, trpB) not found in a CT mouse strain (MoPn) or other species of Chlamydia...
  65. MOLECULAR ANALYSIS OF A POTENTIAL TRANSPORT OPERON IN AA
    Karen Novak; Fiscal Year: 2001
    ..For example, the Bordetella pertussis pertussis toxin liberation (ptl) operon and the Agrobacterium tumefaciens virulence (virB) operon are two well characterized, homologous transport operons...
  66. ANALYSIS TYPHIMURIUM ADHESION TO PEYERS PATCHES
    ANDREAS BAUMLER; Fiscal Year: 2001
    ..The lpf operon encodes long polar fimbriae (LP fimbriae), which allow Salmonella typhimurium to adhere to murine Peyer's patches ..
  67. STREPTOCOCCAL HYALURONATE CAPSULE
    Ivo van de Rijn; Fiscal Year: 1999
    ..Towards this objective they plan to : 1) Isolate and sequence the hyaluronate synthase (has) capsule operon. Determine which genes of the operon are required for production of capsule in group A streptococci and analyze ..
  68. Characterization of iron transport in GBS
    KATHRYN CLANCY; Fiscal Year: 2004
    ..We have identified a putative siderophore-mediated iron transport system, the fhu operon, in GBS...
  69. MOLECULAR MECHANISM OF REGULATION OF THE LUX REGULON
    Thomas Baldwin; Fiscal Year: 1991
    ..The primary regulation involves a positive regulatory protein, LuxR, the sole gene product of the left operon, and a small molecule effector, N-(3-oxohexanoyl)homoserine lactone, or "autoinducer"...
  70. MERCURY RESISTANCE IN STAPHYLOCOCCUS AUREUS
    RICHARD LADDAGA; Fiscal Year: 1992
    ..One of these resistance determinants is the mercury resistance determinant (mer operon) contained on the S. aureus plasmid pI258...
  71. ASSEMBLY OF A MEMBRANE-BOUND MULTI-ENZYME COMPLEX
    JOHN DE MOSS; Fiscal Year: 1993
    We propose to extend our studies on the regulation of the expression of the nar operon and the biogenesis of the nitrate reductase complex in Escherichia coli with the following general aims: (a) To define the molecular mechanisms ..
  72. Genetic circuits based on allosteric ribozymes
    Andrew D Ellington; Fiscal Year: 2010
    ..are instrumental in modulating the expression of operons involved in the utilization of sugars, such as the lac operon. Starting with three different ribozyme platforms (hammerhead, L1 ligase, and Group I self-splicing intron) we ..