genetic recombination

Summary

Summary: Production of new arrangements of DNA by various mechanisms such as assortment and segregation, CROSSING OVER; GENE CONVERSION; GENETIC TRANSFORMATION; GENETIC CONJUGATION; GENETIC TRANSDUCTION; or mixed infection of viruses.

Top Publications

  1. ncbi Generating samples under a Wright-Fisher neutral model of genetic variation
    Richard R Hudson
    Department of Ecology and Evolution, University of Chicago, 1101 E 57th Street, Chicago, IL 60637, USA
    Bioinformatics 18:337-8. 2002
  2. pmc Evaluation of methods for detecting recombination from DNA sequences: computer simulations
    D Posada
    Department of Zoology, Brigham Young University, Provo, UT 84602, USA
    Proc Natl Acad Sci U S A 98:13757-62. 2001
  3. ncbi Mechanism of eukaryotic homologous recombination
    Joseph San Filippo
    Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, CT 06520, USA
    Annu Rev Biochem 77:229-57. 2008
  4. ncbi The genome of the African trypanosome Trypanosoma brucei
    Matthew Berriman
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton CB10 1SA, UK
    Science 309:416-22. 2005
  5. pmc Mauve: multiple alignment of conserved genomic sequence with rearrangements
    Aaron C E Darling
    Department of Computer Science, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    Genome Res 14:1394-403. 2004
  6. pmc A simple and robust statistical test for detecting the presence of recombination
    Trevor C Bruen
    McGill Centre for Bioinformatics, McGill University, Montreal, Quebec, Canada
    Genetics 172:2665-81. 2006
  7. ncbi Automated phylogenetic detection of recombination using a genetic algorithm
    Sergei L Kosakovsky Pond
    Department of Pathology, University of California San Diego, USA
    Mol Biol Evol 23:1891-901. 2006
  8. ncbi Genome maintenance mechanisms for preventing cancer
    J H Hoeijmakers
    MGC Department of Cell Biology and Genetics, Centre for Biomedical Genetics, Erasmus University, PO Box 1738, 3000DR Rotterdam, The Netherlands
    Nature 411:366-74. 2001
  9. ncbi The structure of haplotype blocks in the human genome
    Stacey B Gabriel
    Whitehead MIT Center for Genome Research, Cambridge, MA 02139, USA
    Science 296:2225-9. 2002
  10. pmc Simple and highly efficient BAC recombineering using galK selection
    Søren Warming
    Mouse Cancer Genetics Program, National Cancer Institute Frederick, MD 21702 1201, USA
    Nucleic Acids Res 33:e36. 2005

Detail Information

Publications359 found, 100 shown here

  1. ncbi Generating samples under a Wright-Fisher neutral model of genetic variation
    Richard R Hudson
    Department of Ecology and Evolution, University of Chicago, 1101 E 57th Street, Chicago, IL 60637, USA
    Bioinformatics 18:337-8. 2002
    ..The samples produced can be used to investigate the sampling properties of any sample statistic under these neutral models...
  2. pmc Evaluation of methods for detecting recombination from DNA sequences: computer simulations
    D Posada
    Department of Zoology, Brigham Young University, Provo, UT 84602, USA
    Proc Natl Acad Sci U S A 98:13757-62. 2001
    ..Results shown here will provide some guidance in the selection of the most appropriate method/s for the analysis of the particular data at hand...
  3. ncbi Mechanism of eukaryotic homologous recombination
    Joseph San Filippo
    Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, CT 06520, USA
    Annu Rev Biochem 77:229-57. 2008
    ..Recent progress on elucidating the mechanisms of action of Rad51 and Dmc1 and their cohorts of ancillary factors is reviewed here...
  4. ncbi The genome of the African trypanosome Trypanosoma brucei
    Matthew Berriman
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton CB10 1SA, UK
    Science 309:416-22. 2005
    ..brucei and the greatest in L. major. Horizontal transfer of genes of bacterial origin has contributed to some of the metabolic differences in these parasites, and a number of novel potential drug targets have been identified...
  5. pmc Mauve: multiple alignment of conserved genomic sequence with rearrangements
    Aaron C E Darling
    Department of Computer Science, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    Genome Res 14:1394-403. 2004
    ..We have evaluated the quality of Mauve alignments and drawn comparison to other methods through extensive simulations of genome evolution...
  6. pmc A simple and robust statistical test for detecting the presence of recombination
    Trevor C Bruen
    McGill Centre for Bioinformatics, McGill University, Montreal, Quebec, Canada
    Genetics 172:2665-81. 2006
    ..The results suggest that phi(w) is one of the best approaches to distinguish recurrent mutation from recombination in a wide variety of circumstances...
  7. ncbi Automated phylogenetic detection of recombination using a genetic algorithm
    Sergei L Kosakovsky Pond
    Department of Pathology, University of California San Diego, USA
    Mol Biol Evol 23:1891-901. 2006
    ..Finally, we demonstrate that prescreening alignments with our method allows one to analyze recombinant sequences for positive selection...
  8. ncbi Genome maintenance mechanisms for preventing cancer
    J H Hoeijmakers
    MGC Department of Cell Biology and Genetics, Centre for Biomedical Genetics, Erasmus University, PO Box 1738, 3000DR Rotterdam, The Netherlands
    Nature 411:366-74. 2001
    ..This review summarizes the main DNA caretaking systems and their impact on genome stability and carcinogenesis...
  9. ncbi The structure of haplotype blocks in the human genome
    Stacey B Gabriel
    Whitehead MIT Center for Genome Research, Cambridge, MA 02139, USA
    Science 296:2225-9. 2002
    ..Our results provide a foundation for the construction of a haplotype map of the human genome, facilitating comprehensive genetic association studies of human disease...
  10. pmc Simple and highly efficient BAC recombineering using galK selection
    Søren Warming
    Mouse Cancer Genetics Program, National Cancer Institute Frederick, MD 21702 1201, USA
    Nucleic Acids Res 33:e36. 2005
    ....
  11. pmc Cre reporter strains produced by targeted insertion of EYFP and ECFP into the ROSA26 locus
    S Srinivas
    Department of Genetics and Development, Columbia University, New York, USA
    BMC Dev Biol 1:4. 2001
    ....
  12. ncbi Mobile elements: drivers of genome evolution
    Haig H Kazazian
    Department of Genetics, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Science 303:1626-32. 2004
    ..Although the host can often control their numbers, massive expansions of retrotransposons have been tolerated during evolution. Now mobile elements are becoming useful tools for learning more about genome evolution and gene function...
  13. pmc Human CtIP promotes DNA end resection
    Alessandro A Sartori
    The Wellcome Trust and Cancer Research UK Gurdon Institute, and Department of Zoology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK
    Nature 450:509-14. 2007
    ..These findings establish evolutionarily conserved roles for CtIP-like proteins in controlling DSB resection, checkpoint signalling and homologous recombination...
  14. pmc Sae2, Exo1 and Sgs1 collaborate in DNA double-strand break processing
    Eleni P Mimitou
    Department of Microbiology, Columbia University Medical Center, 701 West 168th Street, New York, New York 10032, USA
    Nature 455:770-4. 2008
    ..Second, Exo1 and/or Sgs1 rapidly process this intermediate to generate extensive tracts of single-stranded DNA that serve as substrate for Rad51...
  15. ncbi DNA double-strand breaks: signaling, repair and the cancer connection
    K K Khanna
    The Queensland Institute of Medical Research, and Department of Pathology, University of Queensland, PO Royal Brisbane Hospital, Brisbane, Queensland, Australia
    Nat Genet 27:247-54. 2001
    ..Moreover, we discuss how tumor suppressor proteins such as p53, ATM, Brca1 and Brca2 have been linked to such pathways, and how accumulating evidence is connecting deficiencies in cellular responses to DNA DSBs with tumorigenesis...
  16. ncbi Sister-scanning: a Monte Carlo procedure for assessing signals in recombinant sequences
    M J Gibbs
    Research School of Biological Sciences, The Australian National University, GPO Box 475, Canberra ACT 2601, Australia
    Bioinformatics 16:573-82. 2000
    ..To devise a method that, unlike available methods, directly measures variations in phylogenetic signals in gene sequences that result from recombination, tests the significance of the signal variations and distinguishes misleading signals...
  17. ncbi A modified bootscan algorithm for automated identification of recombinant sequences and recombination breakpoints
    D P Martin
    Institute of Infectious Diseases and Molecular Medicine, University of Cape Town, Observatory 7925, South Africa
    AIDS Res Hum Retroviruses 21:98-102. 2005
    ....
  18. ncbi The complete genome sequence of Escherichia coli K-12
    F R Blattner
    Laboratory of Genetics, University of Wisconsin Madison, 445 Henry Mall, Madison, WI 53706, USA
    Science 277:1453-62. 1997
    ..The genome also contains insertion sequence (IS) elements, phage remnants, and many other patches of unusual composition indicating genome plasticity through horizontal transfer...
  19. pmc Sex and virulence in Escherichia coli: an evolutionary perspective
    Thierry Wirth
    Department of Molecular Biology, Schumannstrasse 21 22, Max Planck Institut für Infektionsbiologie, 10117 Berlin, Germany
    Mol Microbiol 60:1136-51. 2006
    ..This long-term pattern of evolution was observed in genes distributed throughout the genome, and thereby is the likely result of episodic selection for strains that can escape the host immune response...
  20. ncbi Sensing DNA damage through ATRIP recognition of RPA-ssDNA complexes
    Lee Zou
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Science 300:1542-8. 2003
    ..Our data suggest that RPA-coated ssDNA is the critical structure at sites of DNA damage that recruits the ATR-ATRIP complex and facilitates its recognition of substrates for phosphorylation and the initiation of checkpoint signaling...
  21. pmc Inference of population structure using multilocus genotype data: linked loci and correlated allele frequencies
    Daniel Falush
    Department of Molecular Biology, Max Planck Institut für Infektionsbiologie, Schumann Strasse 21 22, 10117 Berlin, Germany
    Genetics 164:1567-87. 2003
    ..The methods are implemented in a program, structure, version 2.0, which is available at http://pritch.bsd.uchicago.edu...
  22. pmc Pathways of DNA double-strand break repair during the mammalian cell cycle
    Kai Rothkamm
    Fachrichtung Biophysik, Universitat des Saarlandes, D 66421 Homburg Saar, Germany
    Mol Cell Biol 23:5706-15. 2003
    ..These data provide the first evaluation of the cell cycle-specific contributions of NHEJ and HR to the repair of radiation-induced versus replication-associated DSBs...
  23. pmc A highly efficient recombineering-based method for generating conditional knockout mutations
    Pentao Liu
    Mouse Cancer Genetics Program, Center for Cancer Research, National Cancer Institute, Frederick, Maryland 21702, USA
    Genome Res 13:476-84. 2003
    ..This method should also facilitate the generation of knock-in mutations and transgene constructs, as well as expedite the analysis of regulatory elements and functional domains in or near genes...
  24. pmc Genome sequence of the human malaria parasite Plasmodium falciparum
    Malcolm J Gardner
    The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, Maryland 20850, USA
    Nature 419:498-511. 2002
    ..The genome sequence provides the foundation for future studies of this organism, and is being exploited in the search for new drugs and vaccines to fight malaria...
  25. ncbi Crossover/noncrossover differentiation, synaptonemal complex formation, and regulatory surveillance at the leptotene/zygotene transition of meiosis
    G Valentin Börner
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    Cell 117:29-45. 2004
    ....
  26. pmc An exact nonparametric method for inferring mosaic structure in sequence triplets
    Maciej F Boni
    Stanford Genome Technology Center, Palo Alto, California 94304, USA
    Genetics 176:1035-47. 2007
    ....
  27. ncbi Inducible gene targeting in mice
    R Kuhn
    Institute for Genetics, University of Cologne, Germany
    Science 269:1427-9. 1995
    ..Deletion was complete in liver and nearly complete in lymphocytes within a few days, whereas partial deletion was obtained in other tissues. This method can be used for the inducible inactivation of any other gene in vivo...
  28. pmc An efficient recombination system for chromosome engineering in Escherichia coli
    D Yu
    Gene Regulation and Chromosome Biology Laboratory and Mouse Cancer Genetics Program, National Cancer Institute, Division of Basic Science, National Cancer Institute Frederick Cancer Research and Development Center, Frederick, MD 21702, USA
    Proc Natl Acad Sci U S A 97:5978-83. 2000
    ..This system will be especially useful for the engineering of large bacterial plasmids such as those from bacterial artificial chromosome libraries...
  29. ncbi Genome-wide insertional mutagenesis of Arabidopsis thaliana
    Jose M Alonso
    Genomic Analysis Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA
    Science 301:653-7. 2003
    ..Insertion mutations were identified in genes that are regulated in response to the plant hormone ethylene...
  30. pmc A second generation human haplotype map of over 3.1 million SNPs
    Kelly A Frazer
    The Scripps Research Institute, 10550 North Torrey Pines Road MEM275, La Jolla, California 92037, USA
    Nature 449:851-61. 2007
    ..Finally, we demonstrate increased differentiation at non-synonymous, compared to synonymous, SNPs, resulting from systematic differences in the strength or efficacy of natural selection between populations...
  31. pmc Construction of transgenic Drosophila by using the site-specific integrase from phage phiC31
    Amy C Groth
    Department of Genetics, Stanford University School of Medicine, Stanford, California 94305, USA
    Genetics 166:1775-82. 2004
    ..These experiments demonstrate the potential for precise genetic engineering of the Drosophila genome with the phiC31 integrase system and will likely benefit research in Drosophila and other insects...
  32. ncbi Un ménage à quatre: the molecular biology of chromosome segregation in meiosis
    Mark Petronczki
    Research Institute of Molecular Pathology, Dr Bohrgasse 7, 1030 Vienna, Austria
    Cell 112:423-40. 2003
    ....
  33. ncbi DMC1: a meiosis-specific yeast homolog of E. coli recA required for recombination, synaptonemal complex formation, and cell cycle progression
    D K Bishop
    Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, Massachusetts 02138
    Cell 69:439-56. 1992
    ..DMC1 is homologous to yeast's RAD51 gene, supporting the view that mitotic DSB repair has been recruited for use in meiotic chromosome metabolism...
  34. ncbi The Collaborative Cross, a community resource for the genetic analysis of complex traits
    Gary A Churchill
    The Jackson Laboratory, 600 Main Street Bar Harbor, Maine 04609, USA
    Nat Genet 36:1133-7. 2004
    ..The Collaborative Cross will provide a common reference panel specifically designed for the integrative analysis of complex systems and will change the way we approach human health and disease...
  35. ncbi To err (meiotically) is human: the genesis of human aneuploidy
    T Hassold
    Department of Genetics, Case Western Reserve University, 10, 900 Euclid Avenue, Cleveland, Ohio 44106, USA
    Nat Rev Genet 2:280-91. 2001
    ..However, recent molecular and cytogenetic approaches are now beginning to shed light on the non-disjunctional processes that lead to aneuploidy...
  36. pmc DNA cloning using in vitro site-specific recombination
    J L Hartley
    Life Technologies, Inc, Rockville, Maryland 20850, USA
    Genome Res 10:1788-95. 2000
    ..In this paper, we outline the concepts of this approach and provide several examples that highlight some of its potential...
  37. ncbi Meiotic chromosomes: integrating structure and function
    D Zickler
    Institut de Genetique et Microbiologie, Universite Paris Sud, Orsay, France
    Annu Rev Genet 33:603-754. 1999
    ..The current article reviews recent information on these topics in an historical context. This juxtaposition has suggested new relationships between structure and function. Additional issues were addressed in a previous chapter (551)...
  38. pmc XRCC3 promotes homology-directed repair of DNA damage in mammalian cells
    A J Pierce
    Cell Biology Program, Memorial Sloan Kettering Cancer Center and Cornell University Graduate School of Medical Sciences, New York, New York 10021, USA
    Genes Dev 13:2633-8. 1999
    ..These results establish that XRCC3-mediated homologous recombination can reverse DNA damage that would otherwise be mutagenic or lethal...
  39. pmc A coalescent-based method for detecting and estimating recombination from gene sequences
    Gil McVean
    Department of Statistics, University of Oxford, Oxford OX1 3TG, United Kingdom
    Genetics 160:1231-41. 2002
    ..The extremely high level of recombination detected in both HIV1 and HIV2 sequences demonstrates that recombination cannot be ignored in the analysis of viral population genetic data...
  40. ncbi Efficient recombination in diverse tissues by a tamoxifen-inducible form of Cre: a tool for temporally regulated gene activation/inactivation in the mouse
    Shigemi Hayashi
    Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Avenue, Cambridge, Massachusetts 02138, USA
    Dev Biol 244:305-18. 2002
    ..This inducible Cre system will be a broadly useful tool to modulate gene activity in mouse embryos, adults, and culture systems where temporal control is an important consideration...
  41. pmc Hitchhiking under positive Darwinian selection
    J C Fay
    Committee on Genetics, University of Chicago, Chicago, Illinois 60637, USA
    Genetics 155:1405-13. 2000
    ..Application of the H test to published surveys of Drosophila variation reveals an excess of high frequency variants that are likely to have been influenced by positive selection...
  42. pmc Mechanism and regulation of class switch recombination
    Janet Stavnezer
    Department of Molecular Genetics and Microbiology, Program in Immunology and Virology, University of Massachusetts Medical School, Worcester, Massachusetts 01655 012, USA
    Annu Rev Immunol 26:261-92. 2008
    ..These proteins are important for faithful joining of S regions, and in their absence aberrant recombination and chromosomal translocations involving S regions occur...
  43. pmc Evolution of the core and pan-genome of Streptococcus: positive selection, recombination, and genome composition
    Tristan Lefebure
    Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA
    Genome Biol 8:R71. 2007
    ..This study employs comparative evolutionary analyses of 26 Streptococcus genomes to yield an improved understanding of the relative roles of recombination and positive selection in pathogen adaptation to their hosts...
  44. ncbi Regulation of DNA double-strand break repair pathway choice
    Meena Shrivastav
    Department of Molecular Genetics and Microbiology, University of New Mexico School of Medicine and Cancer Center, Albuquerque, NM 87131, USA
    Cell Res 18:134-47. 2008
    ....
  45. ncbi Differential timing and control of noncrossover and crossover recombination during meiosis
    T Allers
    Laboratory of Biochemistry, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892, USA
    Cell 106:47-57. 2001
    ..These results suggest that crossovers are formed by resolution of Holliday junction intermediates, while most noncrossover recombinants arise by a different, earlier pathway...
  46. ncbi A common sequence motif associated with recombination hot spots and genome instability in humans
    Simon Myers
    Broad Institute of Massachusetts Institute of Technology and Harvard, 7 Cambridge Center, Cambridge, Massachusetts 02142, USA
    Nat Genet 40:1124-9. 2008
    ....
  47. ncbi Mechanisms of site-specific recombination
    Nigel D F Grindley
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520 8114, USA
    Annu Rev Biochem 75:567-605. 2006
    ..Details of the site-specific recombination processes have been revealed by recent structural and biochemical studies of members of both families...
  48. ncbi Identification of Holliday junction resolvases from humans and yeast
    Stephen C Y Ip
    Genetic Recombination Laboratory, Cancer Research UK, London Research Institute, Clare Hall Laboratories, South Mimms, Herts EN6 3LD, UK
    Nature 456:357-61. 2008
    ..Recombinant GEN1 and Yen1 resolve Holliday junctions by the introduction of symmetrically related cuts across the junction point, to produce nicked duplex products in which the nicks can be readily ligated...
  49. ncbi Talking about a revolution: The impact of site-specific recombinases on genetic analyses in mice
    Catherine S Branda
    Harvard Medical School, Department of Genetics, 77 Avenue Louis Pasteur, Boston, MA 02115, USA
    Dev Cell 6:7-28. 2004
    ..By enabling high-fidelity DNA modifications to be induced in vitro or in vivo, these systems have incited a wave of new biology, advancing our understanding of gene function, genetic relationships, development, and disease...
  50. ncbi Cotranscriptionally formed DNA:RNA hybrids mediate transcription elongation impairment and transcription-associated recombination
    Pablo Huertas
    Departamento de Genetica, Facultad de Biologia, Universidad de Sevilla, Avenida Reina, Mercedes 6, 41012 Seville, Spain
    Mol Cell 12:711-21. 2003
    ..These data support a model to explain the connection between recombination, transcription, and mRNA metabolism and provide a new perspective to understanding transcription-associated recombination...
  51. ncbi Rad51 protein involved in repair and recombination in S. cerevisiae is a RecA-like protein
    A Shinohara
    Department of Biology, Faculty of Science, Osaka University, Japan
    Cell 69:457-70. 1992
    ..Rad51 protein is also homologous to a meiosis-specific Dmc1 protein of S. cerevisiae...
  52. ncbi The single-end invasion: an asymmetric intermediate at the double-strand break to double-holliday junction transition of meiotic recombination
    N Hunter
    Department of Molecular and Cellular Biology, Harvard University, 7 Divinity Avenue, Cambridge, MA 02138, USA
    Cell 106:59-70. 2001
    ..This feature can explain crossover suppression between homeologous and structurally heterozygous chromosomes...
  53. ncbi The effect of recombination on the accuracy of phylogeny estimation
    David Posada
    Department of Zoology, Brigham Young University, Provo, UT 84602, USA
    J Mol Evol 54:396-402. 2002
    ..In this case, when the recombinational breakpoint divided the alignment in two regions of similar length, a phylogeny that was different from any of the true phylogenies underlying the data was inferred...
  54. ncbi GARD: a genetic algorithm for recombination detection
    Sergei L Kosakovsky Pond
    Department of Pathology, University of California San Diego, La Jolla, CA 92093, USA
    Bioinformatics 22:3096-8. 2006
    ..The evolution of recombinant sequences can not be properly explained by a single phylogenetic tree, but several phylogenies may be used to correctly model the evolution of non-recombinant fragments...
  55. pmc CtIP-BRCA1 modulates the choice of DNA double-strand-break repair pathway throughout the cell cycle
    Maximina H Yun
    Division of Protein and Nucleic Acid Chemistry, MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, UK
    Nature 459:460-3. 2009
    ....
  56. pmc A cladistic analysis of phenotypic associations with haplotypes inferred from restriction endonuclease mapping and DNA sequence data. III. Cladogram estimation
    A R Templeton
    Department of Biology, Washington University, St Louis, Missouri 63130
    Genetics 132:619-33. 1992
    ..We apply this estimation procedure to three data sets to illustrate varying degrees of cladogram ambiguity and recombination...
  57. ncbi Fork reversal and ssDNA accumulation at stalled replication forks owing to checkpoint defects
    Jose M Sogo
    Institute of Cell Biology, ETH Honggerberg, CH 8093 Zurich, Switzerland
    Science 297:599-602. 2002
    ..We speculate that, in checkpoint mutants, abnormal replication intermediates begin to form because of uncoordinated replication and are further processed by unscheduled recombination pathways, causing genome instability...
  58. ncbi Four evolutionary strata on the human X chromosome
    B T Lahn
    Howard Hughes Medical Institute, Whitehead Institute, and Department of Biology, Massachusetts Institute of Technology, 9 Cambridge Center, Cambridge, MA 02142, USA
    Science 286:964-7. 1999
    ..The first event, which marked the beginnings of X-Y differentiation, occurred about 240 to 320 million years ago, shortly after divergence of the mammalian and avian lineages...
  59. ncbi Ubc9- and mms21-mediated sumoylation counteracts recombinogenic events at damaged replication forks
    Dana Branzei
    FIRC Institute of Molecular Oncology Foundation and Department of Biomedical Sciences and Biotechnology, Universita degli Studi di Milano, Via Adamello 16, 20139 Milan, Italy
    Cell 127:509-22. 2006
    ..Our results indicate that Ubc9- and Mms21-mediated sumoylation functions as a regulatory mechanism, different from that of replication checkpoints, to prevent pathological accumulation of cruciform structures at damaged forks...
  60. pmc Construction of a 10,000-marker ultradense genetic recombination map of potato: providing a framework for accelerated gene isolation and a genomewide physical map
    Hans van Os
    Laboratory of Plant Breeding, Wageningen University, The Netherlands
    Genetics 173:1075-87. 2006
    ..potatogenome.net)...
  61. ncbi Synaptonemal complex assembly in C. elegans is dispensable for loading strand-exchange proteins but critical for proper completion of recombination
    MONICA P COLAIACOVO
    Department of Developmental Biology, Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA
    Dev Cell 5:463-74. 2003
    ..Our data also suggest that early prophase barriers to utilization of sister chromatids as repair templates do not depend on central region assembly...
  62. ncbi The RAD51 gene family, genetic instability and cancer
    John Thacker
    Medical Research Council, Radiation and Genome Stability Unit, Harwell, Oxfordshire OX11 0RD, UK
    Cancer Lett 219:125-35. 2005
    ....
  63. ncbi Class switch recombination and hypermutation require activation-induced cytidine deaminase (AID), a potential RNA editing enzyme
    M Muramatsu
    Department of Medical Chemistry, Graduate School of Medicine, Institute for Virus Research, Kyoto University, Japan
    Cell 102:553-63. 2000
    ..These results suggest that AID may be involved in regulation or catalysis of the DNA modification step of both class switching and somatic hypermutation...
  64. ncbi Mechanism of homologous recombination from the RecA-ssDNA/dsDNA structures
    Zhucheng Chen
    Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Nature 453:489-4. 2008
    ..The underwound, stretched filament conformation probably evolved to destabilize the donor duplex, freeing the complementary strand for homology sampling...
  65. ncbi Generating crossovers by resolution of nicked Holliday junctions: a role for Mus81-Eme1 in meiosis
    Fekret Osman
    Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, United Kingdom
    Mol Cell 12:761-74. 2003
    ..These data demonstrate how crossovers could arise without forming or resolving dHJs using an enzyme that is widely conserved amongst eukaryotes...
  66. pmc Paired-end mapping reveals extensive structural variation in the human genome
    Jan O Korbel
    Molecular Biophysics and Biochemistry Department, Yale University, New Haven, CT 06520, USA
    Science 318:420-6. 2007
    ..The breakpoint junction sequences of more than 200 SVs were determined with a novel pooling strategy and computational analysis. Our analysis provided insights into the mechanisms of SV formation in humans...
  67. ncbi Systematic genetic analysis with ordered arrays of yeast deletion mutants
    A H Tong
    Banting and Best Department of Medical Research, University of Toronto, Toronto ON, Canada M5G 1L6
    Science 294:2364-8. 2001
    ..Systematic application of this approach should produce a global map of gene function...
  68. ncbi Chimeric nucleases stimulate gene targeting in human cells
    Matthew H Porteus
    Department of Biology, California Institute of Technology, Pasadena CA 91125, USA
    Science 300:763. 2003
  69. ncbi Community structure and metabolism through reconstruction of microbial genomes from the environment
    Gene W Tyson
    Department of Environmental Science, Policy and Management, University of California, Berkeley, California 94720, USA
    Nature 428:37-43. 2004
    ..Analysis of the gene complement for each organism revealed the pathways for carbon and nitrogen fixation and energy generation, and provided insights into survival strategies in an extreme environment...
  70. pmc How clonal is Staphylococcus aureus?
    Edward J Feil
    Department of Biology and Biochemistry, University of Bath, Claverton Down, Bath, United Kingdom
    J Bacteriol 185:3307-16. 2003
    ....
  71. ncbi Molecular mechanisms of mammalian DNA repair and the DNA damage checkpoints
    Aziz Sancar
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599 7260, USA
    Annu Rev Biochem 73:39-85. 2004
    ..In this review the molecular mechanisms of DNA repair and the DNA damage checkpoints in mammalian cells are analyzed...
  72. ncbi P[acman]: a BAC transgenic platform for targeted insertion of large DNA fragments in D. melanogaster
    Koen J T Venken
    Program in Developmental Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Science 314:1747-51. 2006
    ..This transgenesis platform should greatly facilitate structure/function analyses of most Drosophila genes...
  73. ncbi Mechanism and control of meiotic recombination initiation
    S Keeney
    Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, and Weill Graduate School of Medical Sciences of Cornell University, New York, New York 10021, USA
    Curr Top Dev Biol 52:1-53. 2001
    ....
  74. ncbi Insights into DNA recombination from the structure of a RAD51-BRCA2 complex
    Luca Pellegrini
    University of Cambridge, Department of Biochemistry, Tennis Court Road, Cambridge CB2 1GA, UK
    Nature 420:287-93. 2002
    ..Cancer-associated mutations that affect the BRC repeat disrupt its predicted interaction with RAD51, yielding structural insight into mechanisms for cancer susceptibility...
  75. ncbi Two-step red-mediated recombination for versatile high-efficiency markerless DNA manipulation in Escherichia coli
    B Karsten Tischer
    Cornell University, Ithaca, NY, USA
    Biotechniques 40:191-7. 2006
    ....
  76. ncbi EXO1-A multi-tasking eukaryotic nuclease
    Phuoc T Tran
    Graduate Medical Education, St Mary s Medical Center, San Francisco, CA 94117, USA
    DNA Repair (Amst) 3:1549-59. 2004
    ..Here, we review studies in several experimental systems that shed light on the role of Exo1 in these DNA transaction pathways, particularly those that may relate to oncogenesis...
  77. ncbi A fine-scale map of recombination rates and hotspots across the human genome
    Simon Myers
    Department of Statistics, University of Oxford, 1 South Parks Road, Oxford OX1 3TG, UK
    Science 310:321-4. 2005
    ....
  78. pmc RTEL1 maintains genomic stability by suppressing homologous recombination
    Louise J Barber
    DNA Damage Response Laboratory, London Research Institute, Cancer Research UK, Clare Hall, South Mimms EN6 3LD, UK
    Cell 135:261-71. 2008
    ..We propose that loss of HR control after deregulation of RTEL1 may be a critical event that drives genome instability and cancer...
  79. ncbi Role of Mre11 in chromosomal nonhomologous end joining in mammalian cells
    Emilie Rass
    Unité mixte de recherche 217, Centre National de la Recherche Scientifique Commissariat à l Energie Atomique, Equipe labellisée LA LIGUE 2008, Institut de Radiobiologie Cellulaire et Moleculaire, Fontenay aux Roses, France
    Nat Struct Mol Biol 16:819-24. 2009
    ..These data demonstrate that, in addition to its role in ATM activation, Mre11 can favor alternative NHEJ through its nuclease activity...
  80. pmc Detecting a local signature of genetic hitchhiking along a recombining chromosome
    Yuseob Kim
    Department of Evolutionary Biology, University of Munich, 80333 Munich, Germany
    Genetics 160:765-77. 2002
    ..This method also allows us to estimate the strength and the location of directional selection from DNA sequence data...
  81. ncbi Recombination and linkage disequilibrium in Arabidopsis thaliana
    Sung Kim
    Molecular and Computational Biology, University of Southern California, Los Angeles, California 90089, USA
    Nat Genet 39:1151-5. 2007
    ..LD also reflects the action of selection, and it is more extensive between nonsynonymous polymorphisms than between synonymous polymorphisms...
  82. ncbi Highly efficient endogenous human gene correction using designed zinc-finger nucleases
    Fyodor D Urnov
    Sangamo BioSciences, Inc, Pt Richmond Tech Center 501, Canal Blvd, Suite A100 Richmond, California 94804, USA
    Nature 435:646-51. 2005
    ..We observe comparably high frequencies in human T cells, raising the possibility of strategies based on zinc-finger nucleases for the treatment of disease...
  83. pmc Histone H3 lysine 4 trimethylation marks meiotic recombination initiation sites
    Valerie Borde
    Institut Curie Centre de Recherche, UMR7147 CNRS, Universite Pierre et Marie Curie, Paris, France
    EMBO J 28:99-111. 2009
    ..Novel perspectives to dissect the various layers of the controls of meiotic DSB formation are discussed...
  84. ncbi An evolutionary view of human recombination
    Graham Coop
    Department of Human Genetics, University of Chicago, 920 East 58th Street, Chicago, Illinois 60637, USA
    Nat Rev Genet 8:23-34. 2007
    ....
  85. ncbi DNA double-strand break repair: from mechanistic understanding to cancer treatment
    Thomas Helleday
    Radiation Oncology and Biology, University of Oxford, Oxford OX3 7LJ, UK
    DNA Repair (Amst) 6:923-35. 2007
    ....
  86. ncbi RDP2: recombination detection and analysis from sequence alignments
    D P Martin
    Institute of Infectious Diseases and Molecular Medicine, University of Cape Town Cape Town 7000, South Africa
    Bioinformatics 21:260-2. 2005
    ....
  87. pmc Recombination patterns in aphthoviruses mirror those found in other picornaviruses
    Livio Heath
    Department of Molecular and Cell Biology, Faculty of Science, University of Cape Town, Rondebosch 7701, South Africa
    J Virol 80:11827-32. 2006
    ....
  88. pmc A simplified system for generating recombinant adenoviruses
    T C He
    The Howard Hughes Medical Institute, 424 North Bond Street, Baltimore, MD 21231, USA
    Proc Natl Acad Sci U S A 95:2509-14. 1998
    ..Homogeneous viruses can be obtained from this procedure without plaque purification. This system should expedite the process of generating and testing recombinant adenoviruses for a variety of purposes...
  89. ncbi Human DNA repair genes
    R D Wood
    Imperial Cancer Research Fund, Clare Hall Laboratories, Blanche Lane, South Mimms, Herts EN6 3LD, UK
    Science 291:1284-9. 2001
    ..Modulation of DNA repair should lead to clinical applications including improvement of radiotherapy and treatment with anticancer drugs and an advanced understanding of the cellular aging process...
  90. pmc High efficiency mutagenesis, repair, and engineering of chromosomal DNA using single-stranded oligonucleotides
    H M Ellis
    Gene Regulation and Chromosome Biology Laboratory, Division of Basic Sciences, National Cancer Institute at Frederick, P.O. Box B, Frederick, MD 21702, USA
    Proc Natl Acad Sci U S A 98:6742-6. 2001
    ..Beta protein and ssDNA may prove generally applicable for repairing DNA in many organisms...
  91. ncbi The origins of genome complexity
    Michael Lynch
    Department of Biology, Indiana University, Bloomington, IN 47405, USA
    Science 302:1401-4. 2003
    ..The enormous long-term effective population sizes of prokaryotes may impose a substantial barrier to the evolution of complex genomes and morphologies...
  92. ncbi Hybrid origin of SIV in chimpanzees
    Elizabeth Bailes
    University of Nottingham, Nottingham NG72UH, UK
    Science 300:1713. 2003
  93. ncbi Nonhomologous end joining in yeast
    James M Daley
    Cellular and Molecular Biology Program, University of Michigan Medical School, Ann Arbor, Michigan 48109 0602, USA
    Annu Rev Genet 39:431-51. 2005
    ..We also consider how the balance between NHEJ and homologous repair is regulated by cell state to promote genome preservation. The principles discussed are instructive to NHEJ in all organisms...
  94. ncbi Transgeneration memory of stress in plants
    Jean Molinier
    Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, CH 4058 Basel, Switzerland
    Nature 442:1046-9. 2006
    ..We conclude that environmental factors lead to increased genomic flexibility even in successive, untreated generations, and may increase the potential for adaptation...
  95. pmc Chk2 phosphorylation of BRCA1 regulates DNA double-strand break repair
    Junran Zhang
    Department of Radiation Oncology, Massachusetts General Hospital Harvard Medical School, 149 13th Street, Charlestown, MA 02129, USA
    Mol Cell Biol 24:708-18. 2004
    ..We propose a dual regulatory role for BRCA1 in maintaining genome integrity, whereby BRCA1 phosphorylation status controls the selectivity of repair events dictated by HR and error-prone NHR...
  96. ncbi The cell-cycle checkpoint kinase Chk1 is required for mammalian homologous recombination repair
    Claus Storgaard Sørensen
    Danish Cancer Society, Institute of Cancer Biology, DK 2100 Copenhagen, Denmark
    Nat Cell Biol 7:195-201. 2005
    ..These results highlight a crucial role for the Chk1 signalling pathway in protecting cells against lethal DNA lesions through regulation of HRR...
  97. ncbi Chromosomal translocations in yeast induced by low levels of DNA polymerase a model for chromosome fragile sites
    Francene J Lemoine
    Department of Biology and Curriculum in Genetics and Molecular Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Cell 120:587-98. 2005
    ..We demonstrate that this pair of Ty elements constitutes a preferred site for double-strand DNA breaks when DNA replication is compromised, analogous to the fragile sites observed in mammalian chromosomes...
  98. pmc Distinct histone modifications define initiation and repair of meiotic recombination in the mouse
    Jerome Buard
    Institut de Genetique Humaine, CNRS UPR 1142, Montpellier, France
    EMBO J 28:2616-24. 2009
    ..Altogether, the chromatin signatures of the Psmb9 and Hlx1 hotspots provide a basis for understanding the distribution of meiotic recombination...
  99. pmc Hierarchy of nonhomologous end-joining, single-strand annealing and gene conversion at site-directed DNA double-strand breaks
    Wael Y Mansour
    Laboratory of Radiobiology and Experimental Radiation Oncology, Department of Radiotherapy and Radiation Oncology, University Medical School Hamburg Eppendorf, Martinistrasse 52, D 20246 Hamburg, Germany
    Nucleic Acids Res 36:4088-98. 2008
    ..Furthermore, the cellular choice of repair pathways is reversible and can be influenced at the level of effector proteins such as Ku80 or Rad51...
  100. ncbi Requirement of NAD and SIR2 for life-span extension by calorie restriction in Saccharomyces cerevisiae
    S J Lin
    Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Science 289:2126-8. 2000
    ..These findings suggest that the increased longevity induced by calorie restriction requires the activation of Sir2p by NAD...
  101. ncbi Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous system
    Jean Livet
    Department of Molecular and Cellular Biology and Center for Brain Science, Harvard University, Cambridge, Massachusetts 02138, USA
    Nature 450:56-62. 2007
    ..The ability of the Brainbow system to label uniquely many individual cells within a population may facilitate the analysis of neuronal circuitry on a large scale...

Research Grants70

  1. Variation in M. tuberculosis in response to host selection
    Sarah Fortune; Fiscal Year: 2009
    ..In these studies, we expect to provide fundamental insights into the mechanisms and targets of diversifying immune selection in M. tuberculosis. ..
  2. Role of the Y-Located TSPY Gene in Human Oncogenesis
    Yun Fai Chris Lau; Fiscal Year: 2013
    ..This project will shed important insights on how the Y-oncogene and X-tumor suppressor operate and how one can interfere or boost their actions respectively to treat prostate cancer. ..
  3. Genome-wide recombination as a risk factor for nondisjunction
    NATASHA D HOLLIS; Fiscal Year: 2010
    ..The reference letters are strong. She has the necessary package to excel as a pre-doctoral fellow. ..
  4. Regulation of thyroid hormone levels by central and peripheral pathways
    KRISTEN RACHEL VELLA; Fiscal Year: 2013
    ..abstract_text> ..
  5. Cellular &Molecular Defects in Human B Cell Development
    Charlotte Cunningham-Rundles; Fiscal Year: 2013
    ..We hypothesize that mutations in this receptor and its isoforms can be used to elucidate the complex functions of this receptor in a human system. ..
  6. Cre, Dre Dual Lineage Model for Calcium Imaging and Optogenetic Manipulations
    Petr Tvrdik; Fiscal Year: 2013
    ..We will be able to determine if Hoxb8 mutant microglia respond to neural stimuli correctly. Taken together, the proposed work will advance the set of tools available to address the underlying causes of glial and other disorders. ..
  7. Functional Analysis of the Dual Specificity Kinase NEK1 in Mammalian Meiosis
    JOANNA KIM HOLLOWAY; Fiscal Year: 2011
    ..Understanding the role of key genes involved in ensuring proper chromosomal segregation during meiosis will increase our understanding of the mechanisms controlling these events and the complex causes of infertility. ..
  8. Environmental Risk Factors for Copy Number Variation in Human Chromosomes
    THOMAS EDWARD WILSON; Fiscal Year: 2010
    ....
  9. Non-coding RNA engineers antibody diversity
    Uttiya Basu; Fiscal Year: 2011
    ..Antibodies are polypeptide complexes produced from B-lymphocytes that are present in the bodily fluids of vertebrates, and are used by the immune system to identify and neutralize foreign antigens, such as bacteria and viruses. ..
  10. Combined BAC Transgenic and Knock-Out Mouse Model of Lowe Syndrome Nephropathy
    Robert L Nussbaum; Fiscal Year: 2013
    ..Finally, after completing a 10-generation backcross onto C57Bl/6, we will examine the mice for abnormalities in central nervous system by testing motor function, anxiety, learning, memory and by examining the brains neuropathologically. ..
  11. Epigenetic downregulation of the antibody response and inhibition of autoimmunity
    Paolo Casali; Fiscal Year: 2013
    ....
  12. Insights from Comparative Genomics for the Treatment of Pneumococcal Infections
    Natalia Luisa Hiller; Fiscal Year: 2011
    ..PUBLIC HEALTH RELEVANCE: The long-term goal of this proposal is the diagnosis and treatment of bacterial-induced ear infections. These infections are the most common illnesses in infants and young children. ..
  13. Development of a Porcine Model of Atherosclerosis
    Christopher Rogers; Fiscal Year: 2010
    ..This animal model will provide academic and industrial research communities with an opportunity to better understand cardiovascular disease and its pathogenesis, and to develop and test new therapeutics and preventative strategies. ..
  14. Protein composition and interactions of telemeric chromatin
    Michael P Killoran; Fiscal Year: 2010
    ..Together, a model for telomere breakdown and large-scale approaches for studying chromatin dynamics at telomeres will make a strong contribution to understanding genome instability in cancer formation. ..
  15. Testing the Therapeutic Potential of iPS Cells for Inherited Skin Diseases
    Jakub Tolar; Fiscal Year: 2013
    ..If the studies outlined in this application are successful, these pre-clinical data will pave the way for approval of iPSC-based clinical trials for not only other forms of EB, but also other inherited skin disorders. ..
  16. Insights from Comparative Genomics for the Treatment of Pneumococcal Infections
    Natalia Luisa Hiller; Fiscal Year: 2013
    ..abstract_text> ..
  17. Regulation of EBV Latency by EBNA1
    Paul M Lieberman; Fiscal Year: 2013
    ..More detailed understanding of EBNA1 is further justified by its potential value as a target for therapeutic intervention in diseases and cancers associated with EBV latent infection. ..
  18. NOX1 and NOX2 as Therapeutic Targets in Influenza
    JOHN DAVID LAMBETH; Fiscal Year: 2013
    ....
  19. Deriving hepatocytes from disease specific iPS to treat metabolic liver disorders
    Namita Roy-Chowdhury; Fiscal Year: 2013
    ....
  20. Genetic Analysis of FAK Activity
    David D Schlaepfer; Fiscal Year: 2012
    ..These studies will fill key gaps in our understanding of the basic signaling events regulating cell movement and underlying processes such as tumor spread. ..
  21. Inactivation of Hdac3 in the cause, prevention, and treatment of HCC
    Scott W Hiebert; Fiscal Year: 2013
    ..This application addresses the role of this key gene in the cause, prevention, and treatment of hepatocellular carcinoma. ..
  22. Chromosomal Control of Segregation of C. Elegans
    Abby F Dernburg; Fiscal Year: 2013
    ..Through these approaches, we will learn how this molecular machine assembles from its component parts to govern the reassortment of genetic traits during sexual reproduction. ..
  23. Identifying Key Structural Elements in the Activation of the Arp2/3 Complex
    Patricia A Khuu; Fiscal Year: 2013
    ..Arp2/3 is also involved in metastasis as it is required in the formation of cellular structures such as lamellipodia of migrating cells and invadopodia of cancers cells [5, 6]. ..
  24. Development of a Porcine Model of Ataxia-Telangiectasia
    Christopher Rogers; Fiscal Year: 2011
    ..This project is relevant to the NIH's mission because it will provide a resource to stimulate discovery, therapeutic application, and the development of new diagnostic tools. ..
  25. Novel therapies for Fanconi anemia
    DAVID A contact WILLIAMS; Fiscal Year: 2010
    ....
  26. Functional Analysis of the Dual Specificity Kinase NEK1 in Mammalian Meiosis
    JOANNA KIM HOLLOWAY; Fiscal Year: 2013
    ..My progress in this direction will be assessed by regular meetings with my co-mentors, inter-departmental seminars on my research and, ultimately, by my publication record. ..
  27. CSHL 2010 Conference on Molecular Genetics and Bacteria & Phages
    David J Stewart; Fiscal Year: 2010
    ..This international conference will provide a forum for scientists working on related topics to share their latest discoveries and will bring together the leading experts in the field. ..
  28. Mouse Models of Non-Syndromic Cardiac Progeria
    Ali J Marian; Fiscal Year: 2012
    ..The models could afford the opportunity to delineate the molecular and cellular mechanisms of cardiolaminopathy and shed light into the mechanisms of cardiovascular senescence. ..
  29. Inducible recombination and gene expression in specific neurotransmitter systems
    Steven A Thomas; Fiscal Year: 2012
    ..It is expected that the proposed mouse models will permit significant insights into the etiology, identification and treatment of these disorders. ..
  30. Regulation of HIV-1 by Rad51 in CNS cells
    Kamel Khalili; Fiscal Year: 2013
    ..The identification of novel host-viral interaction pathways can provide important information regarding the pathogenesis of AIDS/CNS disease and promote the development of more effective therapeutic strategies. ..
  31. Cellular functions of the essential PcrA helicase in Staphylococcus aureus
    Saleem A Khan; Fiscal Year: 2013
    ..aureus. Since PcrA is a conserved protein, the knowledge gained from these studies could be used in the future for the development of drugs against S. aureus and other Gram-positive human pathogens. ..
  32. Regulation of the VDJ recombinase during genotoxic stress
    Karla K Rodgers; Fiscal Year: 2013
    ..The ability to enhance such a regulatory process may lead to decreased risks of developing lymphoid malignancies following exposure to radiation and other DNA damaging drugs. ..
  33. Activating liver carcinogens in yeast by expressing CYP450 polymorphisms
    MICHAEL THOMAS FASULLO; Fiscal Year: 2010
    ..specific aim, we will determine whether a subset of CYP1A1 and CYP1A2 polymorphisms affect frequencies of genetic recombination and mutation and the DNA damage response to AFB1 and liver carcinogens...
  34. Is symmetrical dimethylation of Arginine 2 of Histone H3 used as an epigenetic si
    Marjorie A Oettinger; Fiscal Year: 2010
    ..The experiments proposed here should define the roles of this modification and lead to insights into its possible role in both normal development and human disease. ..
  35. Modeling schizophrenia gamma deficits using cell-specific RNAi knockdown of GAD67
    RITCHIE EDWARD BROWN; Fiscal Year: 2012
    ....
  36. 2012 CSHL Bacteria &Phages Conference
    David J Stewart; Fiscal Year: 2012
    ..This international conference will provide a forum for scientists working on related topics to share their latest discoveries and will bring together the leading experts in the field. ..
  37. ANALYSIS OF NEMATODE SEX DETERMINATION
    Barbara J Meyer; Fiscal Year: 2013
    ..the compaction, resolution, and segregation of mitotic and meiotic chromosomes as well as the control of genetic recombination between homologous meiotic chromosomes...
  38. Novel Bacillus subtilis based spore vaccine to tuberculosis
    Subramanian Dhandayuthapani; Fiscal Year: 2013
    ..Finally, we will test whether mice with these immunization groups are protected against challenge doses of live M. tuberculosis H37Rv. ..
  39. Cyclin-Dependent Kinase Inhibition During S Phase
    Geoffrey I Shapiro; Fiscal Year: 2013
    ..abstract_text> ..
  40. Generation of Site-Specific Recombinase-Expressing Transgenic Rats using an Enhan
    Eric M Ostertag; Fiscal Year: 2011
    ..The results from this study will generate valuable genetic tools for refined and subtle investigations of gene function in specific tissues. ..
  41. Evolutionary Analysis of the Histone Deacetylase Family: Biomedical Research
    JOSEPH LEWIS GRAVES; Fiscal Year: 2011
    ..This in turn has the potential to inform drug development efforts impacting a breadth of specific diseases, as well as general aging itself. ..
  42. Identification of Disease-Causing Mutations in SCID Using Exome-Wide Sequencing
    Joseph L Roberts; Fiscal Year: 2010
    ....
  43. A Fourth Outcome: DNA Damage and the Differentiation of B Cells
    Michael A Teitell; Fiscal Year: 2013
    ..Overall, we dissect a new and unexpected fourth outcome for DNA damage- cell differentiation. ..
  44. ANALYSIS OF MEIOTIC CHROMOSOME SYNAPSIS IN YEAST
    Nancy M Hollingsworth; Fiscal Year: 2013
    ..The knowledge gained from these studies is likely to be relevant to mammals, given the evolutionary conservation of meiosis between yeast and mammals. ..
  45. Genetic toxicology of purine metabolism
    Peter C Dedon; Fiscal Year: 2010
    ....
  46. Checkpoints and double strand breaks in S. pombe meiosis
    Susan L Forsburg; Fiscal Year: 2013
    ..This will provide important insights into the stresses that impact meiotic progression in humans as well as yeast. ..
  47. Gene Therapy Reduction of Radiotherapy Esophagitis
    Joel S Greenberger; Fiscal Year: 2010
    ..These studies should elucidate the molecular mechanism of induction of damage to engrafting marrow origin, epithelial stem cells by the irradiated esophageal microenvironment and identify new targets for therapeutic intervention. ..
  48. Coronavirus RNA Replication
    DAVID ALVIN BRIAN; Fiscal Year: 2012
    ..Knowing in detail what molecular interactions lead to coronavirus replication and recombination, the focus of this proposal, are essential for the development of stable and safe recombinant vaccines and the design of anti-viral drugs. ..
  49. Genomic profiling of yeast resistance to AFB1, a P450-activated carcinogen
    MICHAEL THOMAS FASULLO; Fiscal Year: 2013
    ..The project will be a valuable training tool in systems and computational biology. ..
  50. Meiosis, SUMOylation and the ZIP3 Protein: Parallel Studies in Mouse and Yeast.
    Neil Hunter; Fiscal Year: 2012
    ..Defects in these processes are linked to human infertility, miscarriage and genetic diseases, particularly cancer. A greater understanding of their mechanism and regulation will help us better understand the etiology of these diseases. ..
  51. The Role of Fibrocytes in Liver Fibrosis
    Tatiana Kisseleva; Fiscal Year: 2012
    ..We expect that mutant collagen-deficient fibrocytes exhibit a defect in transmigration, differentiation into myofibroblasts, while Col1a1rr-mutant fibrocytes are expected to possess a highly fibrogenic phenotype. ..
  52. Mechanics and regulation of chromosome dynamics in meiotic prophase
    MICHAEL EDWARD DRESSER; Fiscal Year: 2012
    ..Understanding this new process will provide a new perspective to understand how chromosomes are prepared for inheritance and what makes chromosome transmission fail so frequently in humans. ..
  53. Long-Range Genetic and Epigenetic Control of Igh Gene Assembly
    Eugene M Oltz; Fiscal Year: 2010
    ..We propose studies to address how precursor B cells control long-range changes in the spatial organization of the immunoglobulin heavy chain locus to ensure its proper assembly and pursuant generation of an adaptive immune system. ..
  54. Retroviral Subversion of ERAD and Intrinsic Immunity
    JAQUELIN PAGE DUDLEY; Fiscal Year: 2013
    ..Further understanding of ERAD and intrinsic immunity are essential to develop new treatments for cancer and pathogenic viral infections. ..
  55. Development of Site-Specific Mutagenesis in Coxiella burnetti
    JAMES EVANS SAMUEL; Fiscal Year: 2012
    ..The proposed project outlined several strategies to develop genetic tools for characterization of gene functions and will aid the development of a vaccine as national security goal. ..
  56. Beta-Globin Gene Correction Using Peptide Nucleic Acids for the Treatment of Sick
    GERALD FRANCIS VOVIS; Fiscal Year: 2010
    ..Helix Therapeutics is proposing to develop a therapeutic gene targeting agent to correct, in human hematopoietic stem cells, the mutation responsible for causing SCD and, thus, cure the disease permanently. ..
  57. Genetic Interactions and Synthetic Population Biology
    Michael B Elowitz; Fiscal Year: 2013
    ..These fundamental issues are critical for understanding the link between population genetics and the observed complex disease phenotypes, and are thus central to the development of genomic medicine. ..
  58. Regulation of Immunoglobulin Class Switch in Aged Mice
    Bonnie B Blomberg; Fiscal Year: 2013
    ..Studies of protein and mRNA stability, post translational regulation, and signal transduction pathways including MAPK, will determine key molecular elements in the control of Ig class switch in aged mice. ..
  59. Triplex-mediated DNA repair as a novel therapy for Hurler Syndrome patients
    Elizabeth Peterson-Roth; Fiscal Year: 2012
    ..Our protocol would repair this mutation in isolated blood stem cells from HS patients and then reintroduce the corrected cells back into the patient with the potential of having a significant impact on the symptoms of the disease. ..
  60. Functional role of RAG2 H3K4me3 interaction in V(D)J recombination
    Marjorie A Oettinger; Fiscal Year: 2013
    ..Our goal is to understand the normal steps in development and how errors in the process lead to immune deficiency syndromes and lymphoid tumors, with the hope of preventing or treating such diseases. ..
  61. INSULIN RECEPTOR STRUCTURE AND TURNOVER
    C Ronald Kahn; Fiscal Year: 2013
    ....
  62. Study DNA repair in preventing MDS and AML after radiation and benzene exposure
    EDWARD PAUL HASTY; Fiscal Year: 2013
    ..adult). Thus, results from this proposal will elucidate the pathobiology of MDS/AML. ..
  63. PAIRING OF HOMOLOGOUS SEQUENCES IN DROSOPHILA
    KENT GOLIC; Fiscal Year: 2002
    ....
  64. Host factors involved in viral RNA recombination
    Peter Nagy; Fiscal Year: 2006
    ..Knowledge of recombination promises untold health benefits by providing means to limit virus infections and to develop safer vaccines. ..
  65. The role of the host Ca/Mn pump in emergence of novel viral RNA recombinants
    Peter Nagy; Fiscal Year: 2010
    ..The research holds promise of benefiting society by leading to groundbreaking results in the area of virus recombination/evolution, host-virus interaction and the emergence of new viruses. ..
  66. Targeted Manipulation of the Zebrafish Genome through Homologous Recombination
    Brian Ciruna; Fiscal Year: 2010
    ....
  67. TEST OF NEUTRAL MOLECULAR EVOLUTION
    Martin Kreitman; Fiscal Year: 1999
    ..Rates of synonymous and amino acid substitutions will be determined for these species pairs, and examined for consistency with the patterns observed for D. melanogaster and D. simulans. ..
  68. Dissecting the mechanism of viral RNA recombination
    Peter Nagy; Fiscal Year: 2004
    ..Knowledge of recombination promises untold health benefits by providing means to limit virus infections and safer vaccines. ..
  69. Molecular Structure of Yeast Chromosome 1
    DAVID KABACK; Fiscal Year: 2005
    ..abstract_text> ..
  70. Rapid genome-wide mapping of mammalian meiotic recombination hotspots.
    PHILIPPE ROGER JEAN BOIS; Fiscal Year: 2010
    ..The research proposed in this R21 will provide fundamental knowledge regarding the roles of meiotic recombination in directing genetic diversity and in driving evolution. ..