post translational protein processing

Summary

Summary: Any of various enzymatically catalyzed post-translational modifications of PEPTIDES or PROTEINS in the cell of origin. These modifications include carboxylation; HYDROXYLATION; ACETYLATION; PHOSPHORYLATION; METHYLATION; GLYCOSYLATION; ubiquitination; oxidation; proteolysis; and crosslinking and result in changes in molecular weight and electrophoretic motility.

Top Publications

  1. ncbi X-linked IAP is a direct inhibitor of cell-death proteases
    Q L Deveraux
    The Burnham Institute, Program on Apoptosis and Cell Death Research, La Jolla, California 92037, USA
    Nature 388:300-4. 1997
  2. ncbi Protein modification by SUMO
    Erica S Johnson
    Department of Biochemistry and Molecular Pharmacology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    Annu Rev Biochem 73:355-82. 2004
  3. ncbi Global, in vivo, and site-specific phosphorylation dynamics in signaling networks
    Jesper V Olsen
    Center for Experimental Bioinformatics, Department of Biochemistry and Molecular Biology, University of Southern Denmark, DK 5230 Odense, Denmark
    Cell 127:635-48. 2006
  4. ncbi Proteomic analysis of post-translational modifications
    Matthias Mann
    Center for Experimental Bioinformatics, Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, Odense M, DK 5230 Denmark
    Nat Biotechnol 21:255-61. 2003
  5. ncbi Concepts in sumoylation: a decade on
    Ruth Geiss-Friedlander
    Department of Biochemie I, Faculty of Medicine, University of Goettingen, Humboldt Allee 23, 37073 Goettingen, Germany
    Nat Rev Mol Cell Biol 8:947-56. 2007
  6. ncbi Prediction of post-translational glycosylation and phosphorylation of proteins from the amino acid sequence
    Nikolaj Blom
    Center for Biological Sequence Analysis, The Technical University of Denmark, Lyngby, Denmark
    Proteomics 4:1633-49. 2004
  7. ncbi The DNA damage response: ten years after
    J Wade Harper
    Department of Pathology, Harvard Medical School, and Center for Genetics and Genomics, Brigham and Women s Hospital, Boston, MA 02115, USA
    Mol Cell 28:739-45. 2007
  8. ncbi Demethylation of H3K27 regulates polycomb recruitment and H2A ubiquitination
    Min Gyu Lee
    Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA
    Science 318:447-50. 2007
  9. pmc Global analysis of protein palmitoylation in yeast
    Amy F Roth
    Department of Pharmacology, Wayne State University School of Medicine, Detroit, MI 48201, USA
    Cell 125:1003-13. 2006
  10. ncbi Mdm2 is a RING finger-dependent ubiquitin protein ligase for itself and p53
    S Fang
    Laboratory of Immune Cell Biology, Division of Basic Sciences, NCI, National Institutes of Health, Bethesda, Maryland 20892 1152, USA
    J Biol Chem 275:8945-51. 2000

Detail Information

Publications372 found, 100 shown here

  1. ncbi X-linked IAP is a direct inhibitor of cell-death proteases
    Q L Deveraux
    The Burnham Institute, Program on Apoptosis and Cell Death Research, La Jolla, California 92037, USA
    Nature 388:300-4. 1997
    ....
  2. ncbi Protein modification by SUMO
    Erica S Johnson
    Department of Biochemistry and Molecular Pharmacology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    Annu Rev Biochem 73:355-82. 2004
    ..This review discusses our current understanding of how SUMO conjugation is controlled, as well as the roles of SUMO in a number of biological processes...
  3. ncbi Global, in vivo, and site-specific phosphorylation dynamics in signaling networks
    Jesper V Olsen
    Center for Experimental Bioinformatics, Department of Biochemistry and Molecular Biology, University of Southern Denmark, DK 5230 Odense, Denmark
    Cell 127:635-48. 2006
    ..The dynamic phosphoproteome provides a missing link in a global, integrative view of cellular regulation...
  4. ncbi Proteomic analysis of post-translational modifications
    Matthias Mann
    Center for Experimental Bioinformatics, Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, Odense M, DK 5230 Denmark
    Nat Biotechnol 21:255-61. 2003
    ..Finally, stable isotope labeling strategies in combination with mass spectrometry have been applied successfully to study the dynamics of modifications...
  5. ncbi Concepts in sumoylation: a decade on
    Ruth Geiss-Friedlander
    Department of Biochemie I, Faculty of Medicine, University of Goettingen, Humboldt Allee 23, 37073 Goettingen, Germany
    Nat Rev Mol Cell Biol 8:947-56. 2007
    ..At first glance, these effects have nothing in common; however, it seems that they all result from changes in the molecular interactions of the sumoylated proteins...
  6. ncbi Prediction of post-translational glycosylation and phosphorylation of proteins from the amino acid sequence
    Nikolaj Blom
    Center for Biological Sequence Analysis, The Technical University of Denmark, Lyngby, Denmark
    Proteomics 4:1633-49. 2004
    ..The new server, NetPhosK, is made publicly available at the URL http://www.cbs.dtu.dk/services/NetPhosK/. The issues of underestimation, over-prediction and strategies for improving prediction specificity are also discussed...
  7. ncbi The DNA damage response: ten years after
    J Wade Harper
    Department of Pathology, Harvard Medical School, and Center for Genetics and Genomics, Brigham and Women s Hospital, Boston, MA 02115, USA
    Mol Cell 28:739-45. 2007
    ..These findings have important implications for aging and cancer...
  8. ncbi Demethylation of H3K27 regulates polycomb recruitment and H2A ubiquitination
    Min Gyu Lee
    Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA
    Science 318:447-50. 2007
    ..Our results suggest a concerted mechanism for transcriptional activation in which cycles of H3K4 methylation by MLL2/3 are linked with the demethylation of H3K27 through UTX...
  9. pmc Global analysis of protein palmitoylation in yeast
    Amy F Roth
    Department of Pharmacology, Wayne State University School of Medicine, Detroit, MI 48201, USA
    Cell 125:1003-13. 2006
    ....
  10. ncbi Mdm2 is a RING finger-dependent ubiquitin protein ligase for itself and p53
    S Fang
    Laboratory of Immune Cell Biology, Division of Basic Sciences, NCI, National Institutes of Health, Bethesda, Maryland 20892 1152, USA
    J Biol Chem 275:8945-51. 2000
    ..However, this RING was ineffective in ubiquitination and proteasomal targeting of p53, suggesting that there may be specificity at the level of the RING in the recognition of heterologous substrates...
  11. ncbi Post-translational modifications and activation of p53 by genotoxic stresses
    E Appella
    Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Eur J Biochem 268:2764-72. 2001
    ....
  12. ncbi Global histone modification patterns predict risk of prostate cancer recurrence
    David B Seligson
    Department of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California, Los Angeles, California 90095, USA
    Nature 435:1262-6. 2005
    ..Thus, widespread changes in specific histone modifications indicate previously undescribed molecular heterogeneity in prostate cancer and might underlie the broad range of clinical behaviour in cancer patients...
  13. ncbi The nucleoporin RanBP2 has SUMO1 E3 ligase activity
    Andrea Pichler
    Max Planck Institute for Biochemistry, Am Klopferspitz 18a, 82152 Martinsried, Germany
    Cell 108:109-20. 2002
    ..Our findings place SUMOylation at the cytoplasmic filaments of the NPC and suggest that, at least for some substrates, modification and nuclear import are linked events...
  14. ncbi Transcriptional regulation by the phosphorylation-dependent factor CREB
    B Mayr
    Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, California 92037, USA
    Nat Rev Mol Cell Biol 2:599-609. 2001
    ..CREB is phosphorylated in response to various signals, but how is specificity achieved in these signalling pathways?..
  15. ncbi Deficiency of presenilin-1 inhibits the normal cleavage of amyloid precursor protein
    B De Strooper
    Experimental Genetics Group, Flemish Institute for Biotechnology VIB4, Center for Human Genetics, K U Leuven, Belgium
    Nature 391:387-90. 1998
    ..Our results indicate that mutations in PS1 that manifest clinically cause a gain of function and that inhibition of PS1 activity is a potential target for anti-amyloidogenic therapy in Alzheimer's disease...
  16. ncbi Functional heterogeneity of small ubiquitin-related protein modifiers SUMO-1 versus SUMO-2/3
    H Saitoh
    The Picower Institute for Medical Research, Manhasset, New York 11030, USA
    J Biol Chem 275:6252-8. 2000
    ..Together, these results support the concept of important distinctions between the SUMO-2/3 and SUMO-1 conjugation pathways and suggest a role for SUMO-2/3 in the cellular responses to environmental stress...
  17. ncbi Genome-wide patterns of histone modifications in yeast
    Catherine B Millar
    Department of Biological Chemistry, Geffen School of Medicine and the Molecular Biology Institute, University of California, Los Angeles, California 90095, USA
    Nat Rev Mol Cell Biol 7:657-66. 2006
    ..Such patterns might be important for the regulation of heterochromatin-mediated silencing, chromosome segregation, DNA replication and gene expression...
  18. ncbi PTMs on H3 variants before chromatin assembly potentiate their final epigenetic state
    Alejandra Loyola
    CNRS UMR 218, Institut Curie, Paris 75248, France
    Mol Cell 24:309-16. 2006
    ..Although the set of initial modifications present on H3.1 is permissive for further modifications, in H3.3 a subset cannot be K9me3. Thus, initial modifications impact final PTMs within chromatin...
  19. ncbi Notch-1 signalling requires ligand-induced proteolytic release of intracellular domain
    E H Schroeter
    Department of Molecular Biology and Pharmacology, Washington University, St Louis, Missouri 63110, USA
    Nature 393:382-6. 1998
    ..Very small amounts of NICD are active, explaining why it is hard to detect in the nucleus in vivo. We also show that it is ligand binding that induces release of NICD...
  20. ncbi Histone lysine methylation: a signature for chromatin function
    Robert J Sims
    Howard Hughes Medical Institute, Division of Nucleic Acids Enzymology, Department of Biochemistry, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA
    Trends Genet 19:629-39. 2003
  21. ncbi Expression patterns and post-translational modifications associated with mammalian histone H3 variants
    Sandra B Hake
    Laboratory of Chromatin Biology, The Rockefeller University, New York, New York 10021, USA
    J Biol Chem 281:559-68. 2006
    ..1 and H3.2 in mammalian cells should not be treated as equivalent proteins...
  22. ncbi Protein posttranslational modifications: the chemistry of proteome diversifications
    Christopher T Walsh
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA
    Angew Chem Int Ed Engl 44:7342-72. 2005
    ..An understanding of the scope and pattern of the many posttranslational modifications in eukaryotic cells provides insight into the function and dynamics of proteome compositions...
  23. ncbi Palmitoylation of intracellular signaling proteins: regulation and function
    Jessica E Smotrys
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Annu Rev Biochem 73:559-87. 2004
    ..This review discusses the function and regulation of protein palmitoylation, focusing on intracellular proteins that participate in cell signaling or protein trafficking...
  24. ncbi Biochemical analysis of Parkinson's disease-causing variants of Parkin, an E3 ubiquitin-protein ligase with monoubiquitylation capacity
    Cornelia Hampe
    Neurologie et Thérapeutique Expérimentale, INSERM U679 Université Pierre and Marie Curie, Paris, France
    Hum Mol Genet 15:2059-75. 2006
    ..The discovery of monoubiquitylated Parkin species in cells hints at a novel post-translational modification potentially involved in the regulation of Parkin function...
  25. ncbi Protein palmitoylation by a family of DHHC protein S-acyltransferases
    David A Mitchell
    Department of Biochemistry, Medical College of Wisconsin, Milwaukee, WI, USA
    J Lipid Res 47:1118-27. 2006
    ..This review presents the genetic and biochemical evidence for the PAT activity of DHHC proteins and discusses the mechanism of protein-mediated palmitoylation...
  26. ncbi Tyrosine sulfation of the amino terminus of CCR5 facilitates HIV-1 entry
    M Farzan
    Dana Farber Cancer Institute, Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Cell 96:667-76. 1999
    ..CXCR4, another important HIV-1 coreceptor, is also sulfated. Tyrosine sulfation may contribute to the natural function of many 7TMS receptors and may be a modification common to primate immunodeficiency virus coreceptors...
  27. pmc Mass spectrometry analysis of Arabidopsis histone H3 reveals distinct combinations of post-translational modifications
    Lianna Johnson
    Life Science Core Curriculum, Department and Molecular Biology Institute, University of California, Los Angeles, CA 90095, USA
    Nucleic Acids Res 32:6511-8. 2004
    ..The two histone H3 variants, H3.1 and H3.2, also differ in the abundance of silencing and activating marks confirming other studies showing that the replication-independent histone H3 is enriched in active chromatin...
  28. pmc Functional analysis of the roles of posttranslational modifications at the p53 C terminus in regulating p53 stability and activity
    Lijin Feng
    Section of Molecular Biology, Division of Biological Sciences, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0322, USA
    Mol Cell Biol 25:5389-95. 2005
    ....
  29. ncbi A presenilin-1-dependent gamma-secretase-like protease mediates release of Notch intracellular domain
    B De Strooper
    Neuronal Cell Biology and Gene Transfer Laboratory, Flanders Institute for Biotechnology VIB4, Center for Human Genetics, KU Leuven, Belgium
    Nature 398:518-22. 1999
    ..Thus the targeting of gamma-secretase for the treatment of Alzheimer's disease may risk toxicity caused by reduced Notch signalling...
  30. ncbi Modification of proteins by ubiquitin and ubiquitin-like proteins
    Oliver Kerscher
    Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520, USA
    Annu Rev Cell Dev Biol 22:159-80. 2006
    ..The primary aim of this review is to summarize the latest developments in our understanding of the different Ubl-protein modification systems, including the shared and unique features of these related pathways...
  31. ncbi Biosynthesis and mode of action of lantibiotics
    Champak Chatterjee
    Department of Chemistry, University of Illinois at Urbana Champaign, 600 South Mathews Avenue, Urbana, Illinois, USA
    Chem Rev 105:633-84. 2005
  32. ncbi A proteomic analysis of arginine-methylated protein complexes
    Francois Michel Boisvert
    Terry Fox Molecular Oncology Group and Bloomfield Center for Research on Aging, Lady Davis Institute for Medical Research and Department of Oncology, McGill University, Montreal, Quebec H3T 1E2, Canada
    Mol Cell Proteomics 2:1319-30. 2003
    ..These findings provide a basis for the identification of the role of arginine methylation in many cellular processes...
  33. ncbi A transcriptionally [correction of transcriptively] active complex of APP with Fe65 and histone acetyltransferase Tip60
    X Cao
    The Center for Basic Neuroscience, Department of Molecular Genetics, and Howard Hughes Medical Institute, The University of Texas Southwestern Medical Center, Dallas, TX 75390 9111 USA
    Science 293:115-20. 2001
    ..This complex potently stimulates transcription via heterologous Gal4- or LexA-DNA binding domains, suggesting that release of the cytoplasmic tail of APP by gamma-cleavage may function in gene expression...
  34. ncbi Pervasive combinatorial modification of histone H3 in human cells
    Benjamin A Garcia
    Institute for Genomic Biology, University of Illinois at Urbana Champaign, Urbana, Illinois 61801, USA
    Nat Methods 4:487-9. 2007
    ....
  35. ncbi Identification of novel histone post-translational modifications by peptide mass fingerprinting
    Liwen Zhang
    Department of Chemistry, The Ohio State University, Columbus, OH 43210, USA
    Chromosoma 112:77-86. 2003
    ..Consistent with the role of histone H3 lysine 79 methylation in the formation of silent chromatin structure, histone H4 lysine 59 is essential for transcriptional silencing at the yeast silent mating loci and telomeres...
  36. ncbi InsPecT: identification of posttranslationally modified peptides from tandem mass spectra
    Stephen Tanner
    Department of Bioengineering and Computer Science Department, APM 3832, University of California San Diego, 9500 Gilman Drive, La Jolla, California 92093 0114, USA
    Anal Chem 77:4626-39. 2005
    ..The tool was used to identify a number of novel modifications in different data sets, including many phosphopeptides in data provided by Alliance for Cellular Signaling that were missed by other tools...
  37. ncbi Histone modifications in transcriptional regulation
    Shelley L Berger
    The Wistar Institute, 3601 Spruce Street, Philadelphia, Pennsylvania 19104, USA
    Curr Opin Genet Dev 12:142-8. 2002
    ..This multiplicity of modifications, and their occurrence in patterns and dependent sequences, argues persuasively for the existence of a histone code...
  38. ncbi Reading protein modifications with interaction domains
    Bruce T Seet
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto, Ontario M5G 1X5, Canada
    Nat Rev Mol Cell Biol 7:473-83. 2006
    ..We also identify common strategies through which modification-dependent interactions synergize to regulate cell behaviour...
  39. pmc Histone sumoylation is a negative regulator in Saccharomyces cerevisiae and shows dynamic interplay with positive-acting histone modifications
    Dafna Nathan
    Gene Expression and Regulation Program, The Wistar Institute, Philadelphia, Pennsylvania 19104, USA
    Genes Dev 20:966-76. 2006
    ..These results indicate that sumoylation is the first negative histone modification to be identified in S. cerevisiae and further suggest that sumoylation may serve as a general dynamic mark to oppose transcription...
  40. ncbi Different EZH2-containing complexes target methylation of histone H1 or nucleosomal histone H3
    Andrei Kuzmichev
    Robert Wood Johnson Medical School, Howard Hughes Medical Institute and Department of Biochemistry, University of Medicine and Dentistry of New Jersey, Piscataway, NJ 08854, USA
    Mol Cell 14:183-93. 2004
    ..Our data provide evidence for a novel mechanism regulating the substrate specificity of a chromatin-modifying enzyme through disparate translational products of a regulatory subunit...
  41. ncbi HIV-1 Vif blocks the antiviral activity of APOBEC3G by impairing both its translation and intracellular stability
    Kim Stopak
    Gladstone Institute of Virology and Immunology, University of California San Francisco, San Francisco, CA 94143, USA
    Mol Cell 12:591-601. 2003
    ....
  42. ncbi c-Jun and p53 activity is modulated by SUMO-1 modification
    S Muller
    Unite de Recombinaison et Expression Genetique, INSERM Unité 163, Institut Pasteur, 28 rue du Dr Roux, 75724 Paris Cedex 15, France
    J Biol Chem 275:13321-9. 2000
    ..Taken together, these data provide a first link between the SUMO-1 conjugation pathway and the regulation of transcription factors...
  43. ncbi A targeted multienzyme mechanism for selective microtubule polyglutamylation
    Juliette Van Dijk
    CRBM, CNRS, 34293 Montpellier, France
    Mol Cell 26:437-48. 2007
    ..In conclusion, we propose a multienzyme mechanism of polyglutamylation that can explain how the diversity of polyglutamylation on selected types of MTs is controlled at the molecular level...
  44. ncbi Post-translational modification of p53 in tumorigenesis
    Ann M Bode
    The Hormel Institute, University of Minnesota, 801 16th Avenue NE, Austin, Minnesota 55912, USA
    Nat Rev Cancer 4:793-805. 2004
    ..A thorough understanding of p53 will be extremely useful in the development of new strategies for treating and preventing cancer, including restoration of p53 function and selective killing of tumours with mutant TP53...
  45. ncbi Keeping active endogenous retroviral-like elements in check: the epigenetic perspective
    I A Maksakova
    Terry Fox Laboratory, British Columbia Cancer Research Centre, Vancouver, British Columbia, Canada
    Cell Mol Life Sci 65:3329-47. 2008
    ..We also address relevance of research performed in the mouse system to human and challenges associated with studying repetitive families. (Part of a multi-author review)...
  46. pmc The landscape of histone modifications across 1% of the human genome in five human cell lines
    Christoph M Koch
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB101SA, United Kingdom
    Genome Res 17:691-707. 2007
    ..These results provide an overview of the functional relationship among histone modifications and gene expression in human cells...
  47. ncbi The age of crosstalk: phosphorylation, ubiquitination, and beyond
    Tony Hunter
    The Salk Institute for Biological Studies, La Jolla, CA 92037, USA
    Mol Cell 28:730-8. 2007
    ..Particularly prominent are the multiple connections between phosphorylation and ubiquitination, which act either positively or negatively in both directions to regulate these processes...
  48. ncbi The DeltaF508 cystic fibrosis mutation impairs domain-domain interactions and arrests post-translational folding of CFTR
    Kai Du
    Hospital for Sick Children Research Institute, Program in Cell and Lung Biology, University of Toronto, Ontario M5G 1X8, Canada
    Nat Struct Mol Biol 12:17-25. 2005
    ....
  49. pmc Phosphorylation of serine 303 is a prerequisite for the stress-inducible SUMO modification of heat shock factor 1
    Ville Hietakangas
    Turku Centre for Biotechnology, University of Turku and Abo Akademi University and Department of Biochemistry and Food Chemistry, University of Turku, Finland
    Mol Cell Biol 23:2953-68. 2003
    ..A transient phosphorylation-dependent colocalization of HSF1 and SUMO-1 in nuclear stress granules provides evidence for a strictly regulated subnuclear interplay between HSF1 and SUMO...
  50. pmc Multiplex N-terminome analysis of MMP-2 and MMP-9 substrate degradomes by iTRAQ-TAILS quantitative proteomics
    Anna Prudova
    Department of Biochemistry and Molecular Biology, Centre for Blood Research, University of British Columbia, 4 401 Life Sciences Institute, 2350 Health Sciences Mall, Vancouver, British Columbia V6T 1Z3, Canada
    Mol Cell Proteomics 9:894-911. 2010
    ..Hence, N-terminomics analyses using iTRAQ-TAILS links gelatinases with new mechanisms of action in angiogenesis and reveals unpredicted restrictions in substrate repertoires for these two very similar proteases...
  51. pmc Dual modification of BMAL1 by SUMO2/3 and ubiquitin promotes circadian activation of the CLOCK/BMAL1 complex
    Jiwon Lee
    School of Biological Sciences, Seoul National University, Seoul 151 742, Republic of Korea
    Mol Cell Biol 28:6056-65. 2008
    ..These results indicate that dual modification of BMAL1 by SUMO2/3 and ubiquitin is essential for circadian activation and degradation of the CLOCK/BMAL1 complex...
  52. ncbi LAT palmitoylation: its essential role in membrane microdomain targeting and tyrosine phosphorylation during T cell activation
    W Zhang
    Section on Lymphocyte Signaling, Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892 5430, USA
    Immunity 9:239-46. 1998
    ..LAT is central to T cell activation and is a novel linker molecule shown to require targeting to membrane microdomains for signaling...
  53. pmc Emerging extranuclear roles of protein SUMOylation in neuronal function and dysfunction
    Stephane Martin
    MRC Centre for Synaptic Plasticity, Department of Anatomy, School of Medical Sciences, University of Bristol, University Walk, Bristol, BS8 1TD, UK
    Nat Rev Neurosci 8:948-59. 2007
    ....
  54. ncbi Protein degradation and protection against misfolded or damaged proteins
    Alfred L Goldberg
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 426:895-9. 2003
    ..A full understanding of the pathogenesis of the protein-folding diseases will require greater knowledge of how misfolded proteins are recognized and selectively degraded...
  55. ncbi Abnormal histone modification patterns in lupus CD4+ T cells
    Nan Hu
    Department of Dermatology, Second Xiangya Hospital, Central South University, Changsha, China
    J Rheumatol 35:804-10. 2008
    ..To investigate alterations in histone modifications in patients with systemic lupus erythematosus (SLE)...
  56. pmc O-GLYCBASE version 4.0: a revised database of O-glycosylated proteins
    R Gupta
    Center for Biological Sequence Analysis, The Technical University of Denmark, DK 2800 Lyngby, Denmark
    Nucleic Acids Res 27:370-2. 1999
    ..Sequence logos representing the acceptor specificity patterns for GalNAc, GlcNAc, mannosyl and xylosyl transferases are shown. The O-GLYCBASE database is available through the WWW at http://www.cbs.dtu.dk/databases/OGLYCBASE/..
  57. ncbi Genome-wide replication-independent histone H3 exchange occurs predominantly at promoters and implicates H3 K56 acetylation and Asf1
    Anne Rufiange
    Centre de recherche en cancérologie de l Université Laval, L Hôtel Dieu de Québec CHUQ, Quebec, Canada
    Mol Cell 27:393-405. 2007
    ..Taken together, our data underline the dynamic nature of replication-independent nucleosome assembly/disassembly, specify a link to transcription, and implicate Asf1 and H3 K56 acetylation...
  58. ncbi Lantibiotics: peptides of diverse structure and function
    Joanne M Willey
    Department of Biology, Hofstra University, Hempstead, New York 11549, USA
    Annu Rev Microbiol 61:477-501. 2007
    ....
  59. ncbi Marking histone H3 variants: how, when and why?
    Alejandra Loyola
    Department of Biochemistry and Molecular Biology, MD Anderson Cancer Center, University of Texas, Houston, TX 77030, USA
    Trends Biochem Sci 32:425-33. 2007
    ..We review and discuss recent knowledge about how the choice and initial modifications of a specific variant might affect PTM states and eventually the final epigenetic state of a chromosomal domain...
  60. ncbi Processing, subcellular localization, and function of 519 (granulysin), a human late T cell activation molecule with homology to small, lytic, granule proteins
    S V Pena
    Department of Pediatrics, Stanford University School of Medicine, CA 94305, USA
    J Immunol 158:2680-8. 1997
    ..Its lytic activity suggests the involvement of 519 in CTL effector function...
  61. ncbi Histone crosstalk between H2B monoubiquitination and H3 methylation mediated by COMPASS
    Jung Shin Lee
    Stowers Institute for Medical Research, 1000 East 50 th Street, Kansas City, MO 64110, USA
    Cell 131:1084-96. 2007
    ..Cps35 is also required for proper H3K79 trimethylation. These findings offer insight into the molecular role of Cps35 in translating the H2B monoubiquitination signal into H3 methylation...
  62. ncbi Phosphorylation of p40AUF1 regulates binding to A + U-rich mRNA-destabilizing elements and protein-induced changes in ribonucleoprotein structure
    Gerald M Wilson
    Department of Biochemistry and Molecular Biology and Center for Fluorescence Spectroscopy, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
    J Biol Chem 278:33039-48. 2003
    ....
  63. ncbi Glutaredoxin: role in reversible protein s-glutathionylation and regulation of redox signal transduction and protein translocation
    Melissa D Shelton
    Department of Pharmacology, School of Medicine, Case Western Reserve University, 2109 Adelbert Road, Cleveland, OH 44106 4965, USA
    Antioxid Redox Signal 7:348-66. 2005
    ..Among many proteins under consideration, actin, protein tyrosine phosphatase-1B, and Ras stand out as the best current examples for establishing this regulatory mechanism...
  64. ncbi Cleavage of cohesin by the CD clan protease separin triggers anaphase in yeast
    F Uhlmann
    Research Institute of Molecular Pathology, Vienna, Austria
    Cell 103:375-86. 2000
    ..Cleavage of Sccl in metaphase arrested cells is sufficient to trigger the separation of sister chromatids and their segregation to opposite cell poles...
  65. pmc Regulation of cellular growth by the Drosophila target of rapamycin dTOR
    H Zhang
    Chiron Corporation, Emeryville, California 94608, USA
    Genes Dev 14:2712-24. 2000
    ..Our results suggest that dTOR regulates growth during animal development by coupling growth factor signaling to nutrient availability...
  66. ncbi The novel human protein arginine N-methyltransferase PRMT6 is a nuclear enzyme displaying unique substrate specificity
    Adam Frankel
    University of Texas M D Anderson Cancer Center, Science Park Research Division, Smithville, Texas 78957, USA
    J Biol Chem 277:3537-43. 2002
    ..This novel human PRMT, which resides solely in the nucleus when fused to the green fluorescent protein, joins a family of enzymes with diverse functions within cells...
  67. ncbi Deoxyhypusine synthase gene is essential for cell viability in the yeast Saccharomyces cerevisiae
    K Sasaki
    Department of Molecular Biology, School of Science, Nagoya University, Japan
    FEBS Lett 384:151-4. 1996
    ..This gene was shown to be an intron-free, single-copy gene, and its product can catalyze the synthesis of deoxyhypusine equally in two precursor forms of eIF-5A, derived from two distinct genes of yeast...
  68. ncbi A novel proteolytic cleavage involved in Notch signaling: the role of the disintegrin-metalloprotease TACE
    C Brou
    Unité de Biologie Moléculaire de l Expression Génique, URA 1773 CNRS, Institut Pasteur, Paris, France
    Mol Cell 5:207-16. 2000
    ..Furthermore, experiments carried out on TACE-/- bone marrow-derived monocytic precursor cells suggest that this metalloprotease plays a prominent role in the activation of the Notch pathway...
  69. ncbi Methylation of SPT5 regulates its interaction with RNA polymerase II and transcriptional elongation properties
    Youn Tae Kwak
    Division of Hematology Oncology, Department of Medicine, University of Texas Southwestern Medical Center, Dallas 75390, USA
    Mol Cell 11:1055-66. 2003
    ..These results suggest that methylation of SPT5 is an important posttranslational modification that is involved in regulating its transcriptional elongation properties in response to viral and cellular factors...
  70. ncbi Crosstalk between SUMO and ubiquitin on PCNA is mediated by recruitment of the helicase Srs2p
    Efterpi Papouli
    Cancer Research UK, Clare Hall Laboratories, Blanche Lane, South Mimms, Herts EN6 3LD, United Kingdom
    Mol Cell 19:123-33. 2005
    ..Our findings suggest a mechanism by which SUMO and ubiquitin cooperatively control the choice of pathway for the processing of DNA lesions during replication...
  71. ncbi A novel heterodimeric cysteine protease is required for interleukin-1 beta processing in monocytes
    N A Thornberry
    Department of Biochemistry, Merck Research Laboratories, Rahway, New Jersey 07065
    Nature 356:768-74. 1992
    ..Selective inhibition of the enzyme in human blood monocytes blocks production of mature IL-1 beta, indicating that it is a potential therapeutic target...
  72. ncbi How chromatin-binding modules interpret histone modifications: lessons from professional pocket pickers
    Sean D Taverna
    Laboratory of Chromatin Biology, The Rockefeller University, New York, New York 10021, USA
    Nat Struct Mol Biol 14:1025-40. 2007
    ..Changes in these interactions may have far-reaching implications for human biology and disease, notably cancer...
  73. ncbi Hedgehog is a signaling protein with a key role in patterning Drosophila imaginal discs
    T Tabata
    Department of Biochemistry and Biophysics, University of California, San Francisco 94143
    Cell 76:89-102. 1994
    ..We propose that the cell-cell communication mediated by hedgehog links the special properties of compartment borders with specification of the proximodistal axis in imaginal development...
  74. pmc Small G proteins in islet beta-cell function
    Anjaneyulu Kowluru
    Department of Pharmaceutical Sciences, Eugene Applebaum College of Pharmacy and Health Sciences, Wayne State University, 259 Mack Avenue, Detroit, MI 48202 3489, USA
    Endocr Rev 31:52-78. 2010
    ..Potential significance of these experimental observations from multiple laboratories and possible avenues for future research in this area of islet research are highlighted...
  75. pmc The histone-like NF-Y is a bifunctional transcription factor
    Michele Ceribelli
    Dipartimento di Scienze Biomolecolari e Biotecnologie, Universita di Milano, Via Celoria 26, 20133 Milano, Italy
    Mol Cell Biol 28:2047-58. 2008
    ..These data indicate that NF-Y is embedded in positive as well as in negative methyl histone marks, serving a dual function in transcriptional regulation, as an activator or as a repressor...
  76. pmc Erythropoietin stimulates phosphorylation and activation of GATA-1 via the PI3-kinase/AKT signaling pathway
    Wei Zhao
    Department of Gene and Cell Medicine, Mount Sinai School of Medicine, New York, NY 10029, USA
    Blood 107:907-15. 2006
    ..These data demonstrate that EpoR signaling phosphorylates GATA-1 and modulates its activity via the PI3-kinase/AKT signaling pathway...
  77. ncbi Acetylation of p53 inhibits its ubiquitination by Mdm2
    Muyang Li
    Institute for Cancer Genetics, and Department of Pathology, College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA
    J Biol Chem 277:50607-11. 2002
    ..This study has significant implications regarding a general mechanism by which protein acetylation modulates ubiquitination-dependent proteasome proteolysis...
  78. ncbi DCAFs, the missing link of the CUL4-DDB1 ubiquitin ligase
    Jennifer Lee
    Department of Pathology and Laboratory Medicine, Weill Medical College of Cornell University, New York, NY 10021, USA
    Mol Cell 26:775-80. 2007
    ..Recent work identified a family of DDB1 and CUL4-associated factors (DCAFs) as substrate receptors, implicating a broad spectrum of cellular processes regulated by CUL4-DDB1...
  79. ncbi A novel juxtamembrane domain isoform of HER4/ErbB4. Isoform-specific tissue distribution and differential processing in response to phorbol ester
    K Elenius
    Department of Surgery, Children s Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 272:26761-8. 1997
    ....
  80. ncbi Parkin mediates nonclassical, proteasomal-independent ubiquitination of synphilin-1: implications for Lewy body formation
    Kah Leong Lim
    Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Neurosci 25:2002-9. 2005
    ..Our results suggest that parkin is a dual-function ubiquitin ligase and that K63-linked ubiquitination of synphilin-1 by parkin may be involved in the formation of Lewy body inclusions associated with PD...
  81. ncbi Isoaspartyl post-translational modification triggers autoimmune responses to self-proteins
    M J Mamula
    Section of Rheumatology, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    J Biol Chem 274:22321-7. 1999
    ..Isoaspartyl linkages within proteins are enhanced in aging and stressed cells and arise under physiological conditions. These post-translationally modified peptides may serve as an early immunologic stimulus in autoimmune disease...
  82. ncbi Regulation of p53 responses by post-translational modifications
    Yang Xu
    Section of Molecular Biology, Division of Biological Sciences, University of California, San Diego, USA
    Cell Death Differ 10:400-3. 2003
  83. pmc The zinc finger protein A20 targets TRAF2 to the lysosomes for degradation
    Lianyun Li
    Building 5, Room 433, Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health 5, Center Drive, Bethesda, MD 20892, USA
    Biochim Biophys Acta 1793:346-53. 2009
    ..Our findings suggest a novel mode of A20 action that involves lysosomal targeting of signal molecules bound to A20...
  84. ncbi Analysis of dynamic changes in post-translational modifications of human histones during cell cycle by mass spectrometry
    Debora Bonenfant
    Novartis Institutes for BioMedical Research, Lichtstrasse 35, CH 4056 Basel, Switzerland
    Mol Cell Proteomics 6:1917-32. 2007
    ..Our results show that mitosis was the period of the cell cycle during which many modifications exhibit dynamic changes...
  85. ncbi Nanotransducers in cellular redox signaling: modification of thiols by reactive oxygen and nitrogen species
    Chris E Cooper
    Dept of Biological Sciences, University of Essex, Wivenhoe Park, CO4 3SQ, Colchester, UK
    Trends Biochem Sci 27:489-92. 2002
    ..The findings offer the enticing possibility that the cell can distinguish between different degrees of oxidant and nitrosative exposure by modification at a single site on a signaling molecule...
  86. pmc A statistics-based platform for quantitative N-terminome analysis and identification of protease cleavage products
    Ulrich auf dem Keller
    Department of Biochemistry and Molecular Biology, Centre for Blood Research, University of British Columbia, 4 401 Life Sciences Institute, 2350 Health Sciences Mall, Vancouver, British Columbia V6T 1Z3, Canada
    Mol Cell Proteomics 9:912-27. 2010
    ....
  87. ncbi Crosstalk among Histone Modifications
    Tamaki Suganuma
    Stowers Institute for Medical Research, Kansas City, MO 64110, USA
    Cell 135:604-7. 2008
    ..Recent studies (Duncan et al., 2008; Lee et al., 2007; Li et al., 2008) reveal that regulation of histone modifications can be functionally linked to reinforce the activation or repression of gene expression...
  88. pmc Assembly and misassembly of cystic fibrosis transmembrane conductance regulator: folding defects caused by deletion of F508 occur before and after the calnexin-dependent association of membrane spanning domain (MSD) 1 and MSD2
    Meredith F N Rosser
    Department of Cell and Developmental Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Mol Biol Cell 19:4570-9. 2008
    ..Early folding defects are evident upon translation of the NBD1 and R-domain and are sensed by the RMA-1 ubiquitin ligase complex...
  89. ncbi A ncRNA modulates histone modification and mRNA induction in the yeast GAL gene cluster
    Jonathan Houseley
    Wellcome Trust Centre for Cell Biology, Institute for Cell Biology, University of Edinburgh, Edinburgh EH9 3JR, UK
    Mol Cell 32:685-95. 2008
    ..These chromatin modifications act principally through the Rpd3S complex to aid glucose repression of GAL1-10 at physiologically relevant sugar concentrations...
  90. pmc Molecular implementation and physiological roles for histone H3 lysine 4 (H3K4) methylation
    Ali Shilatifard
    Stowers Institute for Medical Research, 1000 East 50th Street, Kansas City, MO 64110, USA
    Curr Opin Cell Biol 20:341-8. 2008
    ....
  91. ncbi Evidence for the existence of an HP1-mediated subcode within the histone code
    Gwen Lomberk
    Gastroenterology Research Unit, Department of Medicine, and Mayo Clinic Cancer Center, Rochester, MN 55605, USA
    Nat Cell Biol 8:407-15. 2006
    ....
  92. pmc A novel membrane-associated metalloprotease, Ste24p, is required for the first step of NH2-terminal processing of the yeast a-factor precursor
    K Fujimura-Kamada
    Department of Cell Biology and Anatomy, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Cell Biol 136:271-85. 1997
    ..Ste24p and the proteins related to it define a new subfamily of proteins that are likely to function as intracellular, membrane-associated zinc metalloproteases...
  93. ncbi Structural insights into microtubule function
    E Nogales
    Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California 94720, USA
    Annu Rev Biochem 69:277-302. 2000
    ..The combination of structural, genetic, biochemical, and biophysical data should soon give us a fuller understanding of the exquisite details in the regulation of the microtubule cytoskeleton...
  94. ncbi The Caenorhabditis elegans cell-death protein CED-3 is a cysteine protease with substrate specificities similar to those of the human CPP32 protease
    D Xue
    Howard Hughes Medical Institute, Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Genes Dev 10:1073-83. 1996
    ..Our results suggest that different mammalian CED-3/ICE-like proteases may have distinct roles in mammalian apoptosis and that CPP32 is a candidate for being a mammalian functional equivalent of CED-3...
  95. pmc Eaf1 is the platform for NuA4 molecular assembly that evolutionarily links chromatin acetylation to ATP-dependent exchange of histone H2A variants
    Andréanne Auger
    Laval University Cancer Research Center, Hôtel Dieu de Québec CHUQ, 9 McMahon St, Quebec City, Quebec G1R 2J6, Canada
    Mol Cell Biol 28:2257-70. 2008
    ..Our results identified the key central subunit for the structure and functions of the NuA4 histone acetyltransferase complex and functionally linked this activity with the histone variant H2AZ from yeast to human cells...
  96. pmc Predicting protein post-translational modifications using meta-analysis of proteome scale data sets
    Daniel Schwartz
    Department of Genetics, Harvard Medical School, Boston, MA 02115, USA
    Mol Cell Proteomics 8:365-79. 2009
    ..New motif discovery is a byproduct of this approach, and the phosphorylation motif analyses provide strong evidence of evolutionary conservation of both known and novel kinase motifs...
  97. ncbi Loss of acetylation at Lys16 and trimethylation at Lys20 of histone H4 is a common hallmark of human cancer
    Mario F Fraga
    Cancer Epigenetics Laboratory, Molecular Pathology Program, Spanish National Cancer Center, Melchor Fernandez Almagro 3, 28029 Madrid, Spain
    Nat Genet 37:391-400. 2005
    ..Our data suggest that the global loss of monoacetylation and trimethylation of histone H4 is a common hallmark of human tumor cells...
  98. pmc The cyclin-dependent kinase inhibitor KRP2 controls the onset of the endoreduplication cycle during Arabidopsis leaf development through inhibition of mitotic CDKA;1 kinase complexes
    Aurine Verkest
    Department of Plant Systems Biology, Flanders Interuniversity Institute for Biotechnology, Ghent University, B 9052 Gent, Belgium
    Plant Cell 17:1723-36. 2005
    ..Moreover, the proposed model allowed a dynamical simulation of the in vivo observations, validating the sufficiency of the regulatory interactions between CDKA;1, KRP2, and CDKB1;1 in fine-tuning the mitosis-to-endocycle transition...
  99. ncbi Post-translational disruption of dystroglycan-ligand interactions in congenital muscular dystrophies
    Daniel E Michele
    Howard Hughes Medical Institute, Department of Physiology and Biophysics, University of Iowa, Iowa City, Iowa 52242 1101, USA
    Nature 418:417-22. 2002
    ....
  100. ncbi Sorting of lysosomal proteins
    Thomas Braulke
    University Medical Center Hamburg Eppendorf, Department Biochemistry, Children s Hospital Research campus, Martinistr 52, 20246, Hamburg, Germany
    Biochim Biophys Acta 1793:605-14. 2009
    ..The complex interaction of both luminal and cytosolic signals with recognition proteins guarantees the specific and directed transport of proteins to lysosomes...
  101. ncbi Massively regulated genes: the example of TP53
    Monica Hollstein
    LIGHT Laboratories, University of Leeds, Leeds LS2 9JT, UK
    J Pathol 220:164-73. 2010
    ..Presumably this is needed, because the p53 protein can have such profound consequences for a cell...

Research Grants72

  1. Personalized Clinical Diagnostics and Beyond: Integrated Ring Resonator Arrays
    Ryan Bailey; Fiscal Year: 2009
    ..This technology promises to broadly impact the landscape of the biomedical sciences, both meeting the clinical diagnostic challenges of today and pioneering the paradigm-shifting discoveries of tomorrow. ..
  2. Optimization of DNA Repair by Abh8
    Lauren Endres; Fiscal Year: 2013
    ..Overall, these findings will form the basis of future studies into Abh8 regulation during the DDR. ..
  3. Novel Mitochondrial Mechanisms of Ethanol-Induced Cardiac Injury in Aged Females
    DONNA HOPE KORZICK; Fiscal Year: 2010
    ....
  4. Role of SAMe on UBC9 and sumolyation in liver cancer and alcoholic liver injury
    Maria Lauda Tomasi; Fiscal Year: 2012
    ..Three aims are proposed in this application to test these hypotheses and elucidate how SAMe affects signaling pathways that are important in hepatocarcinogenesis and development of alcoholic liver injury. ..
  5. The role of Fli1 in T cell function and the pathogenesis of lupus
    Tamara K Nowling; Fiscal Year: 2011
    ..Additionally, insight will be gained into the regulation of T cell function in both health and disease with relevance to an array of immune and neoplastic diseases. ..
  6. Bioflavonoid Effects on EGF Signaling and Cell Cycle Pathways in Prostate Cancer
    BARRY MATTHEW MARKAVERICH; Fiscal Year: 2013
    ..These studies may lead to the development of new drugs and/or dietary components for use in cancer prevention or treatment. ..
  7. Regulation of K-Ras by a Farnesyl-electrostatic Switch
    MARK REID PHILIPS; Fiscal Year: 2013
    ..We propose to study the cell biology and physiology of K-Ras phosphorylation to better understand how to exploit this process to develop anti-cancer drugs. ..
  8. Regulation of LPS-mediated HMGB1 Release by Poly (ADP-ribose) Polymerase-1
    RAJESH K ANEJA; Fiscal Year: 2013
    ..Additional studies will be performed to determine the therapeutic window for use of PARP-1 inhibitors in murine sepsis. ..
  9. Improving Epigenetic-based Cell Reprogramming with Proteasome Inhibition
    Jaroslaw Staszkiewicz; Fiscal Year: 2011
    ....
  10. Regulation of Triglyceride Metabolism by the transcription factor CREB-H
    Ann Hwee Lee; Fiscal Year: 2013
    ....
  11. The Effects of PP2A on TNF Signaling and Smoke-Induced Lung Injury
    Robert F Foronjy; Fiscal Year: 2013
    ..Importantly, our findings will have important implications for other diseases where TNF signaling and inflammation play a central role. ..
  12. Enhance Production of Functional Recombinant Human Protein C Variant In Mammalian
    Nienwen Chow; Fiscal Year: 2010
    ..This application seeks to develop a potential new therapeutic designed to protect blood vessels as well as neurons in the ALS-affected regions. ..
  13. Evaluating the role of histone H3K79 methylation states in DNA damage repair in S
    Jeffrey S Thompson; Fiscal Year: 2010
    ..Such experiences will inspire students to pursue graduate/professional studies in biomedical research, in addition to the contributions that these students will make towards the scientific goals of this project. ..
  14. ROLE OF SAMe IN LIVER FUNCTION AND INJURY
    SHELLY CHI LOO LU; Fiscal Year: 2012
    ..The goal of this project is to understand how SAMe controls these processes and how injury and cancer occur when SAMe metabolism is perturbed. ..
  15. Proteomic signatures of an early life asthma-protective exposure
    Serrine S Lau; Fiscal Year: 2010
    ....
  16. Varicella zoster virus: molecular controls of cell fusion-dependent pathogenesis
    Ann Arvin; Fiscal Year: 2013
    ....
  17. AMPK Endothelial Cell Dysfunction and the Metabolic Syndrome (PROGRAM PROJECT)
    Neil B Ruderman; Fiscal Year: 2013
    ..The proposed studies should both yield novel insights into the biological bases for the premature atherosclerosis and impaired angiogenesis associated with this entity and suggest new therapeutic targets for their prevention...
  18. Characterization of the thioredoxins system in the diabetic retina
    Folami Lamoke; Fiscal Year: 2013
    ..Aim 2)) Test the hypothesis that HG/diabetes induces TrxR1 protein nitration and this effect contributes to oxidative retinal endothelial cell injury. ..
  19. The Role of the Nuclear Lamina in Chromatin Organization and Gene Expression in N
    Marcos Estecio; Fiscal Year: 2012
    ....
  20. REGULATION OF NUCLEAR ENVELOPE ASSEMBLY AND DISASSEMBLY
    Katherine L Wilson; Fiscal Year: 2012
    ..Aim 3 is a whole-genome and promoter-specific analysis of BAF function in C. elegans, which is expected to identify genes relevant to its roles in muscle and DNA- damage responses. ..
  21. Effect of Maspin on Corneal Heme-and lymph- angiogenesis
    Sally S Twining; Fiscal Year: 2013
    ..The results will guide the generation and initial testing of maspin mutants and peptides as potential modalities for the treatment of neovascular diseases. ..
  22. HMG in Post Operative and Traumatic Stress
    Kevin J Tracey; Fiscal Year: 2013
    ....
  23. MECHANISMS OF LIVER INJURY BY HEPATITIS C AND ALCOHOL
    Steven A Weinman; Fiscal Year: 2013
    ....
  24. Resolving the role of nicotine-mediated phosphorylation on pancreatic fibrosis
    Joao A Paulo; Fiscal Year: 2013
    ..abstract_text> ..
  25. Functional analysis of mDia formins in hematopoietic stem cell engraftment and mi
    Peng Ji; Fiscal Year: 2010
    ..Understanding these processes is highly relevant for bone-marrow transplantation therapies to treat blood-related diseases. ..
  26. Sex Differences in Stress Receptors Underlie Female Vulnerability to Stress
    Debra A Bangasser; Fiscal Year: 2012
    ..This project will not only help us understand why women are more vulnerable to stress-related mental illnesses, but it will identify new pharmaceutical targets which can lead to treatments that are efficacious in both men and women. ..
  27. Modulation of Ca Control of Cardiac Myofibrils
    R John Solaro; Fiscal Year: 2013
    ....
  28. Molecular Analysis of Alcohol Effects on Human Oral Epithelium Cells'Epigenome
    Alison Urvalek; Fiscal Year: 2013
    ....
  29. Enzymology of Post-translational Modifications
    Carol A Fierke; Fiscal Year: 2013
    ..Identification of the protein substrates and downstream biological function will enhance the development of new therapeutics targeting these pathways. ..
  30. Molecular Mechanisms of Mitigation of Insulin Induction of SREBP-1c by N-3 PUFA
    Marshall B Elam; Fiscal Year: 2013
    ..Understanding how PUFA causes this will lead to more effective treatments for high triglyceride levels in individuals with obesity and type II diabetes to reduce risk of heart attack and stroke. ..
  31. PCAF/GCN5 ACETYLATION ON P53 TRANSACTIVATION
    Shelley L Berger; Fiscal Year: 2013
    ..The proposed studies will advance our understanding of how p53 protects cells, and our results will raise the likelihood for pharmacologic regulation of p53 function to fight human cancer. ..
  32. Developing Nrf2 as a target for treatment of corticosteroid resistance in COPD
    Robert A Wise; Fiscal Year: 2012
    ..This projects aims at preclinical development of a potential drug target that can be used in the clinical setting to improve steroid responsiveness in COPD nafients. ..
  33. The functions of the US3 protein kinase of herpes simplex virus
    Bernard Roizman; Fiscal Year: 2011
    ....
  34. REPAIR OF CARCINOGEN DAMAGED DNA IN HUMAN CHROMATIN
    John J Wyrick; Fiscal Year: 2013
    ..As all eukaryotes, including humans, must deal with this 'packaging paradox'for surveillance of the genome, results from these studies are relevant to the broad spectrum of cancer etiology, prevention and treatment. ..
  35. Photocleavable Bead Technology for Glycomics
    Mark Lim; Fiscal Year: 2013
    ..Blixt and Costello). ..
  36. 5500 Q-Trap Mass Spectrometer
    Stephen Barnes; Fiscal Year: 2010
    ..It's very well suited to obtaining quantitative data to better understand the role of the protein(s) in specific areas of public health. ..
  37. ELEVATED CALCIUM AND MYOCARDIAL ISCHEMIC INJURY
    Charles Steenbergen; Fiscal Year: 2013
    ....
  38. Role of POZ-zinc finger proteins in tumorigenesis.
    Pier Paolo Pandolfi; Fiscal Year: 2013
    ..In view of the critical role exerted by this protein in tumorigenesis and embryonic development the further elucidation of POKEMON/LRF function and role in human cancer is of great relevance to biomedical research. ..
  39. Targeting Sirtuin-1 to enhance Foxp3+ regulatory T-cell function
    Ulf Beier; Fiscal Year: 2013
    ..Furthermore, it is important to learn more about the role of Sirtuin-1 in the immune system, as many investigators pursue Sirtuin-1 enhancement (diabetes mellitus, metabolic syndrome), or Sirtuin-1 suppression (certain cancers). ..
  40. Mechanisms that Regulate Neonatal Lung Heme Oxygenase in Hyperoxia
    Phyllis A Dennery; Fiscal Year: 2013
    ....
  41. Analysis and Therapy of Age-Dependent Proteostasis Failure in Neurodegeneration
    ROBERT JOSEPH SHMOOKLER REIS; Fiscal Year: 2013
    ..Proteins or modifications in either soluble or insoluble aggregates, which vary concordantly with neurotoxicity, become functional candidates ..
  42. Molecular Mechanisms in Bone Resorption
    Steven A Lietman; Fiscal Year: 2012
    ..New information about mechanisms of bone resorption may facilitate the development of novel strategies to decrease bone resorption and may lead to new treatments for osteoporosis. ..
  43. FAMU RCMI Pharmaceutical Research Center
    Karam F A Soliman; Fiscal Year: 2013
    ..These efforts will result in positive health outcomes in addition to decreasing and eliminating health disparities. ..
  44. Analysis of PTH and Dopamine Receptor Signaling in Proximal Tubules
    Eleanor D Lederer; Fiscal Year: 2013
    ..Thus understanding how the kidney regulates phosphate homeostasis has significant relevance to the health of Veterans of all ages. ..
  45. Effects of ozone exposure on expression and function of surfactant protein D
    Angela Haczku; Fiscal Year: 2010
    ..Results from this application will define the implications of SP-D in O3-induced exacerbation of asthma and will provide novel approaches to manipulate the pulmonary immune system. ..
  46. System for Rapid Generation of Aptamers for Transplant Therapies
    KAREN QIAN; Fiscal Year: 2010
    ....
  47. Role of FoxOs in Skeletal Homeostasis
    MARIA JOSE ALMEIDA; Fiscal Year: 2013
    ..Increased understanding of the mechanisms that control bone formation will provide important information for the development of therapies to maintain or increase bone mass and strength, thereby reducing the risk of osteoporotic fractures ..
  48. Regulation of cholesterol catabolism by bile acids
    Jongsook Kim Kemper; Fiscal Year: 2013
    ..These studies will elucidate how SHP epigenetically controls BA-regulated hepatic functions, and identify novel potential diagnostic and/or therapeutic targets for metabolic diseases. ..
  49. Molecular elucidation of the CBM complex in NF-kappaB activation by antigen recep
    Hao Wu; Fiscal Year: 2013
    ..These translocations likely activate NF-kB through MALT1 and Bcl10 overexpression. Collectively, these studies suggest that the CBM complex is an attractive therapeutic target neoplastic disorders of the lymphocytes. ..
  50. Gene Regulation by and of LEDGF
    Dhirendra P Singh; Fiscal Year: 2012
    ....
  51. Post Translational Modifications in Tolerance and Autoimmunity
    Mark J Mamula; Fiscal Year: 2012
    ..The goal of this work is to determine the importance of spontaneous protein modification in B and T cell immune tolerance and autoimmune pathology. ..
  52. Urine biomarkers of lupus nephritis pathology and response to therapy
    James C Oates; Fiscal Year: 2013
    ..The long-term goal is to provide clinicians with a qualified biomarker panel coupled with a web-based predictive tool for more accurate diagnosis and therapeutic monitoring of LN. ..
  53. Recombination Repair Complex in Human Cells
    Richard Fishel; Fiscal Year: 2013
    ....
  54. The role of intracellular trafficking pathways in calcium signaling
    Suzette Farber-Katz; Fiscal Year: 2013
    ..The characterization of these proteins will lead to the elucidation of novel pathways that control calcium signaling. ..
  55. Effects of Dietary Fat on Contractile and Mitochondrial Function in Heart Failure
    Margaret P Chandler; Fiscal Year: 2010
    ....
  56. Sex Differences in Stress Receptors Underlie Female Vulnerability to Stress
    Debra A Bangasser; Fiscal Year: 2013
    ..Moreover, because CRF antagonists are being developed to treat these illnesses, considering sex differences in CRF receptors may increase the efficacy of these compounds in women. ..
  57. Distance-Dependent Structure and Function of Neuronal Dendrites
    Kristen M Harris; Fiscal Year: 2010
    ....
  58. Chemistry and biology of protein histidine phosphorylation
    Tom Muir; Fiscal Year: 2013
    ....
  59. The role of a novel AIF-associated nuclease PAAN1 in neuronal injury
    Yingfei Wang; Fiscal Year: 2013
    ..This training will allow me to expand my expertise and help my transition into a successful independent academic researcher. ..
  60. Chromatin Immunoprecipitation (ChIP) Assay Development for FFPE human brain secti
    Mary Anne Jelinek; Fiscal Year: 2011
    ....
  61. Protein biomarkers of pulmonary hypertension in sickle cell disease
    Mark E McComb; Fiscal Year: 2013
    ....
  62. Role of the Giant Protein Titin in Skeletal Muscle Structure and Function
    Henk L Granzier; Fiscal Year: 2013
    ..We hope that ultimately larger muscle masses can be maintained as a result of our studies on the role of titin in hypertrophy signaling. ..
  63. Reading the histone code:nanoscale morphology of Epigneomic Histone Modifications
    M Mitchell Smith; Fiscal Year: 2010
    ....
  64. Multiplex Fluorescent Zdyes for Differential Glycomic Detection
    EDWARD DRATZ; Fiscal Year: 2009
    ....
  65. Humanizing N-linked glycosylation in Escherichia coli
    ADAM FISHER; Fiscal Year: 2009
    ..Therefore, the focus of these studies is the development of "glyco-engineered" bacteria that are capable of producing authentic human glycoproteins. ..
  66. Cone snail venom peptides that modulate zebrafish spinal motor activity
    Joseph Schulz; Fiscal Year: 2009
    ..The peptides will be studied using the zebrafish model system to understand how the normal activity of neurons is regulated with the potential for the development of novel therapeutics to treat nervous system disorders. ..
  67. REGULATION OF PROLYL HYDROXYLASE
    Richard Berg; Fiscal Year: 1992
    ....
  68. Functional studies of the Fragile X Mental Retardation Protein: switching from re
    Mihaela Mihailescu; Fiscal Year: 2009
    ....
  69. Biological role of modifications to C/EBPbeta-1 and -2
    Linda Sealy; Fiscal Year: 2007
    ..These studies will provide important insights into how misregulated C/EBPbeta isoform expression may contribute to the development of metastatic mammary carcinoma. ..
  70. Structure and Mechanism of Pathogen SET Domain HKMTs
    Ming Ming Zhou; Fiscal Year: 2009
    ....
  71. SIGNIFICANCE OF GGT EXPRESSION IN TUMORS
    MARIE HANIGAN; Fiscal Year: 2009
    ..Identifying these pathways and other proteins that are similarly modified will provide novel targets for diagnosis and therapy. ..
  72. Myocardial Ischemia-Reperfusion and Protein Phosphatase 2a
    Polly A Hofmann; Fiscal Year: 2010
    ..the heart, can we improve the short and long term outcomes from ischemic events in the heart? Which of the unique PP2aB targeting subunits is key to improving post-ischemic recovery in the heart, and by what mechanisms does this occur? ..