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| post translational protein processingSummarySummary: Any of various enzymatically catalyzed post-translational modifications of PEPTIDES or PROTEINS in the cell of origin. These modifications include carboxylation; HYDROXYLATION; ACETYLATION; PHOSPHORYLATION; METHYLATION; GLYCOSYLATION; ubiquitination; oxidation; proteolysis; and crosslinking and result in changes in molecular weight and electrophoretic motility. Top Publications
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Publications
How chromatin-binding modules interpret histone modifications: lessons from professional pocket pickersSean D Taverna
Laboratory of Chromatin Biology, The Rockefeller University, New York, New York 10021, USA
Nat Struct Mol Biol 14:1025-40. 2007..Changes in these interactions may have far-reaching implications for human biology and disease, notably cancer...- Global, in vivo, and site-specific phosphorylation dynamics in signaling networksJesper V Olsen
Center for Experimental Bioinformatics, Department of Biochemistry and Molecular Biology, University of Southern Denmark, DK-5230 Odense, Denmark
Cell 127:635-48. 2006..The dynamic phosphoproteome provides a missing link in a global, integrative view of cellular regulation... - Concepts in sumoylation: a decade onRuth Geiss Friedlander
Department of Biochemie I, Faculty of Medicine, University of Goettingen, Humboldt Allee 23, 37073 Goettingen, Germany
Nat Rev Mol Cell Biol 8:947-56. 2007..At first glance, these effects have nothing in common; however, it seems that they all result from changes in the molecular interactions of the sumoylated proteins... - Demethylation of H3K27 regulates polycomb recruitment and H2A ubiquitinationMin Gyu Lee
Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA
Science 318:447-50. 2007..Our results suggest a concerted mechanism for transcriptional activation in which cycles of H3K4 methylation by MLL2/3 are linked with the demethylation of H3K27 through UTX... - Prediction of post-translational glycosylation and phosphorylation of proteins from the amino acid sequenceNikolaj Blom
Center for Biological Sequence Analysis, The Technical University of Denmark, Lyngby, Denmark
Proteomics 4:1633-49. 2004..The new server, NetPhosK, is made publicly available at the URL http://www.cbs.dtu.dk/services/NetPhosK/. The issues of underestimation, over-prediction and strategies for improving prediction specificity are also discussed... - A novel heterodimeric cysteine protease is required for interleukin-1 beta processing in monocytesN A Thornberry
Department of Biochemistry, Merck Research Laboratories, Rahway, New Jersey 07065
Nature 356:768-74. 1992..Selective inhibition of the enzyme in human blood monocytes blocks production of mature IL-1 beta, indicating that it is a potential therapeutic target... - Genome-wide patterns of histone modifications in yeastCatherine B Millar
Department of Biological Chemistry, Geffen School of Medicine and the Molecular Biology Institute, University of California, Los Angeles, California 90095, USA
Nat Rev Mol Cell Biol 7:657-66. 2006..Such patterns might be important for the regulation of heterochromatin-mediated silencing, chromosome segregation, DNA replication and gene expression... - Protein modification by SUMOErica S Johnson
Department of Biochemistry and Molecular Pharmacology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
Annu Rev Biochem 73:355-82. 2004..This review discusses our current understanding of how SUMO conjugation is controlled, as well as the roles of SUMO in a number of biological processes... - X-linked IAP is a direct inhibitor of cell-death proteasesQ L Deveraux
The Burnham Institute, Program on Apoptosis and Cell Death Research, La Jolla, California 92037, USA
Nature 388:300-4. 1997.... 
Functional analysis of the roles of posttranslational modifications at the p53 C terminus in regulating p53 stability and activityLijin Feng
Section of Molecular Biology, Division of Biological Sciences, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0322, USA
Mol Cell Biol 25:5389-95. 2005....- Global analysis of protein palmitoylation in yeastAmy F Roth
Department of Pharmacology, Wayne State University School of Medicine, Detroit, MI 48201, USA
Cell 125:1003-13. 2006.... - Biosynthesis and mode of action of lantibioticsChampak Chatterjee
Department of Chemistry, University of Illinois at Urbana-Champaign, 600 South Mathews Avenue, Urbana, Illinois, USA
Chem Rev 105:633-84. 2005 - InsPecT: identification of posttranslationally modified peptides from tandem mass spectraStephen Tanner
Department of Bioengineering and Computer Science Department, APM 3832, University of California San Diego, 9500 Gilman Drive, La Jolla, California 92093 0114, USA
Anal Chem 77:4626-39. 2005..The tool was used to identify a number of novel modifications in different data sets, including many phosphopeptides in data provided by Alliance for Cellular Signaling that were missed by other tools... - Identification of novel histone post-translational modifications by peptide mass fingerprintingLiwen Zhang
Department of Chemistry, The Ohio State University, Columbus, OH 43210, USA
Chromosoma 112:77-86. 2003..Consistent with the role of histone H3 lysine 79 methylation in the formation of silent chromatin structure, histone H4 lysine 59 is essential for transcriptional silencing at the yeast silent mating loci and telomeres... - Protein posttranslational modifications: the chemistry of proteome diversificationsChristopher T Walsh
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA
Angew Chem Int Ed Engl 44:7342-72. 2005..An understanding of the scope and pattern of the many posttranslational modifications in eukaryotic cells provides insight into the function and dynamics of proteome compositions... - Modification of proteins by ubiquitin and ubiquitin-like proteinsOliver Kerscher
Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520, USA
Annu Rev Cell Dev Biol 22:159-80. 2006..The primary aim of this review is to summarize the latest developments in our understanding of the different Ubl-protein modification systems, including the shared and unique features of these related pathways... - Marking histone H3 variants: how, when and why?Alejandra Loyola
Department of Biochemistry and Molecular Biology, MD Anderson Cancer Center, University of Texas, Houston, TX 77030, USA
Trends Biochem Sci 32:425-33. 2007..We review and discuss recent knowledge about how the choice and initial modifications of a specific variant might affect PTM states and eventually the final epigenetic state of a chromosomal domain... 
Massively regulated genes: the example of TP53Monica Hollstein
LIGHT Laboratories, University of Leeds, Leeds LS2 9JT, UK
J Pathol 220:164-73. 2010..Presumably this is needed, because the p53 protein can have such profound consequences for a cell...- Post-translational disruption of dystroglycan-ligand interactions in congenital muscular dystrophiesDaniel E Michele
Howard Hughes Medical Institute, Department of Physiology and Biophysics, University of Iowa, Iowa City, Iowa 52242 1101, USA
Nature 418:417-22. 2002.... - Structural insights into microtubule functionE Nogales
Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California 94720, USA
Annu Rev Biochem 69:277-302. 2000..The combination of structural, genetic, biochemical, and biophysical data should soon give us a fuller understanding of the exquisite details in the regulation of the microtubule cytoskeleton... - Sorting of lysosomal proteinsThomas Braulke
University Medical Center Hamburg Eppendorf, Department Biochemistry, Children s Hospital Research campus, Martinistr 52, 20246, Hamburg, Germany
Biochim Biophys Acta 1793:605-14. 2009..The complex interaction of both luminal and cytosolic signals with recognition proteins guarantees the specific and directed transport of proteins to lysosomes... - Evidence for the existence of an HP1-mediated subcode within the histone codeGwen Lomberk
Gastroenterology Research Unit, Department of Medicine, and Mayo Clinic Cancer Center, Rochester, MN 55605, USA
Nat Cell Biol 8:407-15. 2006.... - Protein degradation and protection against misfolded or damaged proteinsAlfred L Goldberg
Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
Nature 426:895-9. 2003..A full understanding of the pathogenesis of the protein-folding diseases will require greater knowledge of how misfolded proteins are recognized and selectively degraded... - Regulation of histone methylation by demethylimination and demethylationRobert J Klose
Howard Hughes Medical Institute, Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 7295, USA
Nat Rev Mol Cell Biol 8:307-18. 2007..Here, we examine the enzymatic and structural basis for the mechanisms that these enzymes use to counteract histone methylation and provide insights into their substrate specificity and biological function... - Molecular implementation and physiological roles for histone H3 lysine 4 (H3K4) methylationAli Shilatifard
Stowers Institute for Medical Research, 1000 East 50th Street, Kansas City, MO 64110, USA
Curr Opin Cell Biol 20:341-8. 2008.... - A novel membrane-associated metalloprotease, Ste24p, is required for the first step of NH2-terminal processing of the yeast a-factor precursorK Fujimura Kamada
Department of Cell Biology and Anatomy, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
J Cell Biol 136:271-85. 1997..Ste24p and the proteins related to it define a new subfamily of proteins that are likely to function as intracellular, membrane-associated zinc metalloproteases... - Multiplex N-terminome analysis of MMP-2 and MMP-9 substrate degradomes by iTRAQ-TAILS quantitative proteomicsAnna Prudova
Department of Biochemistry and Molecular Biology, Centre for Blood Research, University of British Columbia, 4 401 Life Sciences Institute, 2350 Health Sciences Mall, Vancouver, British Columbia V6T 1Z3, Canada
Mol Cell Proteomics 9:894-911. 2010..Hence, N-terminomics analyses using iTRAQ-TAILS links gelatinases with new mechanisms of action in angiogenesis and reveals unpredicted restrictions in substrate repertoires for these two very similar proteases... - Predicting protein post-translational modifications using meta-analysis of proteome scale data setsDaniel Schwartz
Department of Genetics, Harvard Medical School, Boston, MA 02115, USA
Mol Cell Proteomics 8:365-79. 2009..New motif discovery is a byproduct of this approach, and the phosphorylation motif analyses provide strong evidence of evolutionary conservation of both known and novel kinase motifs... - The cyclin-dependent kinase inhibitor KRP2 controls the onset of the endoreduplication cycle during Arabidopsis leaf development through inhibition of mitotic CDKA;1 kinase complexesAurine Verkest
Department of Plant Systems Biology, Flanders Interuniversity Institute for Biotechnology, Ghent University, B 9052 Gent, Belgium
Plant Cell 17:1723-36. 2005..Moreover, the proposed model allowed a dynamical simulation of the in vivo observations, validating the sufficiency of the regulatory interactions between CDKA;1, KRP2, and CDKB1;1 in fine-tuning the mitosis-to-endocycle transition... - The Caenorhabditis elegans cell-death protein CED-3 is a cysteine protease with substrate specificities similar to those of the human CPP32 proteaseD Xue
Howard Hughes Medical Institute, Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
Genes Dev 10:1073-83. 1996..Our results suggest that different mammalian CED-3/ICE-like proteases may have distinct roles in mammalian apoptosis and that CPP32 is a candidate for being a mammalian functional equivalent of CED-3... - Loss of acetylation at Lys16 and trimethylation at Lys20 of histone H4 is a common hallmark of human cancerMario F Fraga
Cancer Epigenetics Laboratory, Molecular Pathology Program, Spanish National Cancer Center, Melchor Fernandez Almagro 3, 28029 Madrid, Spain
Nat Genet 37:391-400. 2005..Our data suggest that the global loss of monoacetylation and trimethylation of histone H4 is a common hallmark of human tumor cells... - Eaf1 is the platform for NuA4 molecular assembly that evolutionarily links chromatin acetylation to ATP-dependent exchange of histone H2A variantsAndréanne Auger
Laval University Cancer Research Center, Hôtel Dieu de Québec CHUQ, 9 McMahon St, Quebec City, Quebec G1R 2J6, Canada
Mol Cell Biol 28:2257-70. 2008..Our results identified the key central subunit for the structure and functions of the NuA4 histone acetyltransferase complex and functionally linked this activity with the histone variant H2AZ from yeast to human cells... - Age-related changes in human crystallins determined from comparative analysis of post-translational modifications in young and aged lens: does deamidation contribute to crystallin insolubility?P A Wilmarth
Department of Integrative Biosciences, School of Dentistry, Oregon Health and Science University, 611 South West Campus Drive, Portland, Oregon 97239, USA
J Proteome Res 5:2554-66. 2006..On the basis of spectral counting, the most abundant PTMs in aged lenses were deamidations and methylated cysteines with other PTMs present at lower levels... - Dynamics of component exchange at PML nuclear bodiesStefanie Weidtkamp-Peters
Leibniz Institute of Age Research, Fritz Lipman Institute, Beutenbergstr 11, 07745 Jena, Germany
J Cell Sci 121:2731-43. 2008..These findings provide a kinetics model for factor exchange at PML NBs and highlight potential mechanisms to regulate intranuclear trafficking of specific factors at these domains... - Making copies of chromatin: the challenge of nucleosomal organization and epigenetic informationArmelle Corpet
Laboratory of Nuclear Dynamics and Genome Plasticity, UMR 218 Centre National de la Recherche Scientifique and Institut Curie, 26 Rue d Ulm, Paris Cedex 05, France
Trends Cell Biol 19:29-41. 2009.... - SysPTM: a systematic resource for proteomic research on post-translational modificationsHong Li
Key Lab of Systems Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China
Mol Cell Proteomics 8:1839-49. 2009..SysPTM could be an important contribution to modificomics research. SysPTM is freely available online at www.sysbio.ac.cn/SysPTM... - Production of a DPP activity gradient in the early Drosophila embryo through the opposing actions of the SOG and TLD proteinsG Marques
Department of Molecular Biology and Biochemistry, University of California, Irvine 92697, USA
Cell 91:417-26. 1997..We propose that formation of the embryonic DPP activity gradient involves the opposing effects of SOG inhibiting DPP and TLD processing SOG to release DPP from the inhibitory complex... - Ehrlichia chaffeensis expresses macrophage- and tick cell-specific 28-kilodalton outer membrane proteinsVijayakrishna Singu
Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, 1800 Denison Ave, Manhattan, KS 66506, USA
Infect Immun 73:79-87. 2005..Host cell-specific protein expression coupled with posttranslational modifications may be a hallmark for the pathogen's adaptation to a dual-host life cycle and its persistence... - Epigenetic regulation by histone methylation and histone variantsPeter Cheung
Department of Medical Biophysics, University of Toronto, Ontario Cancer Institute, 610 University Avenue, Room 10 516, Toronto, Ontario, Canada M5G 2M9
Mol Endocrinol 19:563-73. 2005..We also highlight some of the enzymes that mediate histone methylation and discuss the stability and inheritance of this modification... - Regulation of heat shock transcription factor 1 by stress-induced SUMO-1 modificationY Hong
Department of Biochemistry, Chandler Medical Center, University of Kentucky, Lexington, KY 40536, USA
J Biol Chem 276:40263-7. 2001..Mutation of lysine 298 also results in a significant decrease in stress-induced transcriptional activity of HSF1 in vivo. This work implicates SUMO-1 modification as an important modulator of HSF1 function in response to stress... - Post-translational modifications regulate the ticking of the circadian clockMonica Gallego
Center for Children, Huntsman Cancer Institute, Salt Lake City, Utah 84112, USA
Nat Rev Mol Cell Biol 8:139-48. 2007..Mutations in kinases and phosphatases in hamsters, flies, fungi and humans highlight how our timepieces are regulated and provide clues as to how we might be able to manipulate them... - Processing of transforming growth factor beta 1 precursor by human furin convertaseC M Dubois
Department of Pediatrics, Faculty of Medicine, Universite de Sherbrooke, Quebec, Canada
J Biol Chem 270:10618-24. 1995..Collectively, these results provide evidence that in our experimental systems the TGF beta 1 precursor is efficiently and correctly processed by human furin thus permitting release of the biologically active peptide... - The O-GlcNAc transferase gene resides on the X chromosome and is essential for embryonic stem cell viability and mouse ontogenyR Shafi
The Howard Hughes Medical Institute, Glycobiology Research and Training Center, Department of Cellular and Molecular Medicine, 9500 Gilman Drive 0625, University of California San Diego, La Jolla, CA 92093, USA
Proc Natl Acad Sci U S A 97:5735-9. 2000.... - Proteomic analysis of post-translational modificationsMatthias Mann
Center for Experimental Bioinformatics, Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, Odense M, DK 5230 Denmark
Nat Biotechnol 21:255-61. 2003..Finally, stable isotope labeling strategies in combination with mass spectrometry have been applied successfully to study the dynamics of modifications... - Dynamic O-GlcNAc modification of nucleocytoplasmic proteins in response to stress. A survival response of mammalian cellsNatasha E Zachara
Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205-2185, USA
J Biol Chem 279:30133-42. 2004..O-GlcNAc regulates both the rates and extent of the stress-induced induction of heat shock proteins, providing a molecular basis for these findings... - Obesity and impaired prohormone processing associated with mutations in the human prohormone convertase 1 geneR S Jackson
Department of Clinical Biochemistry, Addenbrooke s Hospital, Cambridge, UK
Nat Genet 16:303-6. 1997..In view of the similarity between the proband and the fat/fat mouse phenotype, we infer that molecular defects in prohormone conversion may represent a generic mechanism for obesity, common to humans and rodents... - Rapid PIKK-dependent release of Chk1 from chromatin promotes the DNA-damage checkpoint responseVeronique A J Smits
The Wellcome Trust and Cancer Research UK Gurdon Institute, Tennis Court Road, Cambridge CB2 1QN, United Kingdom
Curr Biol 16:150-9. 2006..Within these pathways, the effector kinase Chk1 plays a central role in mediating cell-cycle arrest in response to DNA damage, and it does so by phosphorylating key cell-cycle regulators... - Deficiency of presenilin-1 inhibits the normal cleavage of amyloid precursor proteinB De Strooper
Experimental Genetics Group, Flemish Institute for Biotechnology VIB4, Center for Human Genetics, K U Leuven, Belgium
Nature 391:387-90. 1998..Our results indicate that mutations in PS1 that manifest clinically cause a gain of function and that inhibition of PS1 activity is a potential target for anti-amyloidogenic therapy in Alzheimer's disease... - Assembly of ER-associated protein degradation in vitro: dependence on cytosol, calnexin, and ATPA A McCracken
University of Nevada, Biology Department, Reno 89557, USA
J Cell Biol 132:291-8. 1996..Together, these results indicate that cytosolic protein factor(s), ATP hydrolysis, and calnexin are required for ER-associated protein degradation in yeast, and suggest the cytosol as the site for degradation... - Notch-1 signalling requires ligand-induced proteolytic release of intracellular domainE H Schroeter
Department of Molecular Biology and Pharmacology, Washington University, St Louis, Missouri 63110, USA
Nature 393:382-6. 1998..Very small amounts of NICD are active, explaining why it is hard to detect in the nucleus in vivo. We also show that it is ligand binding that induces release of NICD... - Hedgehog is a signaling protein with a key role in patterning Drosophila imaginal discsT Tabata
Department of Biochemistry and Biophysics, University of California, San Francisco 94143
Cell 76:89-102. 1994..We propose that the cell-cell communication mediated by hedgehog links the special properties of compartment borders with specification of the proximodistal axis in imaginal development... - SUMOylation and De-SUMOylation: wrestling with life's processesEdward T H Yeh
Department of Cardiology, The University of Texas M D Anderson Cancer Center, Houston, Texas 77030, USA
J Biol Chem 284:8223-7. 2009..This review will focus on the de-SUMOylating enzymes with special attention to their biological function... - Single-nucleosome mapping of histone modifications in S. cerevisiaeChih Long Liu
Bauer Center for Genomics Research, Harvard University, Cambridge, Massachusetts, USA
PLoS Biol 3:e328. 2005..g., promoter nucleosomes) from those at another location (e.g., over the 3' ends of coding regions). These results are consistent with the idea of a simple, redundant histone code, in which multiple modifications share the same role... - Mechanisms involved in the regulation of histone lysine demethylasesFei Lan
Department of Pathology, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, United States
Curr Opin Cell Biol 20:316-25. 2008..The chemical mechanisms and substrate specificities have already been extensively discussed elsewhere. This review focuses primarily on regulatory mechanisms that modulate demethylase recruitment and activity... - Emerging extranuclear roles of protein SUMOylation in neuronal function and dysfunctionStephane Martin
MRC Centre for Synaptic Plasticity, Department of Anatomy, School of Medical Sciences, University of Bristol, University Walk, Bristol, BS8 1TD, UK
Nat Rev Neurosci 8:948-59. 2007.... - Crosstalk among Histone ModificationsTamaki Suganuma
Stowers Institute for Medical Research, Kansas City, MO 64110, USA
Cell 135:604-7. 2008..Recent studies (Duncan et al., 2008; Lee et al., 2007; Li et al., 2008) reveal that regulation of histone modifications can be functionally linked to reinforce the activation or repression of gene expression... - The presenilins in Alzheimer's disease--proteolysis holds the keyC Haass
Adolf Butenandt Institute, Department of Biochemistry, Ludwig Maximilians University Munich, Germany
Science 286:916-9. 1999..These findings suggest that the presenilins may prove to be valuable molecular targets for the development of drugs to combat AD... - Smac, a mitochondrial protein that promotes cytochrome c-dependent caspase activation by eliminating IAP inhibitionC Du
Howard Hughes Medical Institute and Department of Biochemistry, University of Texas Southwestern Medical Center at Dallas, 75235, USA
Cell 102:33-42. 2000..Overexpression of Smac increases cells' sensitivity to apoptotic stimuli. Smac is the second mitochondrial protein, along with cytochrome c, that promotes apoptosis by activating caspases... - OxyR: a molecular code for redox-related signalingSung Oog Kim
Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710, USA
Cell 109:383-96. 2002.... - The DNA damage response: ten years afterJ Wade Harper
Department of Pathology, Harvard Medical School, and Center for Genetics and Genomics, Brigham and Women s Hospital, Boston, MA 02115, USA
Mol Cell 28:739-45. 2007..These findings have important implications for aging and cancer... - Intrinsic disorder and functional proteomicsPredrag Radivojac
School of Informatics, Indiana University, Bloomington, Indiana, USA
Biophys J 92:1439-56. 2007..The future of the prediction of protein disorder and the future uses of such predictions in functional proteomics comprise the last section of this article... - A statistics-based platform for quantitative N-terminome analysis and identification of protease cleavage productsUlrich auf dem Keller
Department of Biochemistry and Molecular Biology, Centre for Blood Research, University of British Columbia, 4 401 Life Sciences Institute, 2350 Health Sciences Mall, Vancouver, British Columbia V6T 1Z3, Canada
Mol Cell Proteomics 9:912-27. 2010.... - Unimod: Protein modifications for mass spectrometryDavid M Creasy
Matrix Science Ltd, London, UK
Proteomics 4:1534-6. 2004..It contains accurate and verifiable values, derived from elemental compositions, for the mass differences introduced by both natural and artificial modifications... - Assembly of cell regulatory systems through protein interaction domainsTony Pawson
Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto, Ontario M5G 1X5, Canada
Science 300:445-52. 2003..The fundamental properties of protein interaction domains are discussed in this review and in detailed reviews on individual domains at Science's STKE at http://www.sciencemag.org/cgi/content/full/300/5618/445/DC1... - S-glutathionylation: indicator of cell stress and regulator of the unfolded protein responseDanyelle M Townsend
Department of Pharmaceutical and Biomedical Sciences, Medical University of South Carolina, Charleston, SC 29425, USA
Mol Interv 7:313-24. 2007..This article seeks to bring together the ways through which these processes are interrelated and considers the implications of these interrelationships upon therapeutic approaches to disease... - Site-specific identification of SUMO-2 targets in cells reveals an inverted SUMOylation motif and a hydrophobic cluster SUMOylation motifIvan Matic
Department of Proteomics and Signal Transduction, Max Planck Institute for Biochemistry, Martinsried D 82152, Germany
Mol Cell 39:641-52. 2010..In 16 proteins we identified a hydrophobic cluster SUMOylation motif (HCSM). SUMO conjugation of RanGAP1 and ZBTB1 via HCSMs is remarkably efficient... - Post-translational protein modifications in antigen recognition and autoimmunityH A Doyle
Yale University School of Medicine, Section of Rheumatology, 333 Cedar Street, LCI 609, PO Box 208031, New Haven, CT 06520 8031, USA
Trends Immunol 22:443-9. 2001..How do post-translational protein modifications affect the processing of foreign and self Ags and what is their role in the origin of autoimmune responses?.. - Nucleosome destabilization in the epigenetic regulation of gene expressionSteven Henikoff
Howard Hughes Medical Institute, Basic Sciences Division, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, Seattle, Washington 98109, USA
Nat Rev Genet 9:15-26. 2008.... - De novo sequencing of unique sequence tags for discovery of post-translational modifications of proteinsYufeng Shen
Biological Science Division, Pacific Northwest National Laboratory, Richland, Washington 99352, USA
Anal Chem 80:7742-54. 2008..The combined de novo-UStag approach complements the UStag method previously reported by enabling the discovery of new protein modifications... - Multisite protein modification and intramolecular signalingXiang Jiao Yang
Molecular Oncology Group, Department of Medicine, McGill University Health Center, Montreal, Quebec, Canada H3A 1A1
Oncogene 24:1653-62. 2005..Multisite modification thus coordinates intermolecular and intramolecular signaling for the qualitative and quantitative control of protein function in vivo... - A transcriptionally [correction of transcriptively] active complex of APP with Fe65 and histone acetyltransferase Tip60X Cao
The Center for Basic Neuroscience, Department of Molecular Genetics, and Howard Hughes Medical Institute, The University of Texas Southwestern Medical Center, Dallas, TX 75390 9111 USA
Science 293:115-20. 2001..This complex potently stimulates transcription via heterologous Gal4- or LexA-DNA binding domains, suggesting that release of the cytoplasmic tail of APP by gamma-cleavage may function in gene expression... - Dual modification of BMAL1 by SUMO2/3 and ubiquitin promotes circadian activation of the CLOCK/BMAL1 complexJiwon Lee
School of Biological Sciences, Seoul National University, Seoul 151 742, Republic of Korea
Mol Cell Biol 28:6056-65. 2008..These results indicate that dual modification of BMAL1 by SUMO2/3 and ubiquitin is essential for circadian activation and degradation of the CLOCK/BMAL1 complex... - Executioner caspase-3 and caspase-7 are functionally distinct proteasesJohn G Walsh
Molecular Cell Biology Laboratory, Department of Genetics, The Smurfit Institute, Trinity College, Dublin 2, Ireland
Proc Natl Acad Sci U S A 105:12815-9. 2008..Our observations provide a molecular basis for the different phenotypes seen in mice lacking either caspase and indicate that these proteases occupy nonredundant roles within the cell death machinery... - Modulation of Ras and a-factor function by carboxyl-terminal proteolysisV L Boyartchuk
Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA
Science 275:1796-800. 1997..Disruption of RCE1 led to defects in Ras localization and signaling and suppressed the activated phenotype associated with the allele RAS2val19... - Histone acetylation and methylation at sites initiating divergent polycistronic transcription in Trypanosoma cruziPatricia Respuela
Department of Genetics and Pathology, Rudbeck Laboratory, Uppsala University, SE 751 85 Uppsala, Sweden
J Biol Chem 283:15884-92. 2008..cruzi with potential implications for transcriptional regulation; they indicate that both histone modifications and bidirectional transcription are evolutionarily conserved... - Autoproteolysis in hedgehog protein biogenesisJ J Lee
Howard Hughes Medical Institute, Department of Molecular Biology and Genetics, Johns Hopkins School of Medicine, Baltimore, MD 21205
Science 266:1528-37. 1994..The two HH protein species exhibited distinctive biochemical properties and tissue distribution, and these differences suggest a mechanism that could account for the long- and short-range signaling activities of HH in vivo... - Analysis of the subcellular localization, function, and proteolytic control of the Arabidopsis cyclin-dependent kinase inhibitor ICK1/KRP1Marc J Jakoby
University group at the Max Planck Institute for Plant Breeding, Max Delbrück Laboratorium, Department of Botany III, University of Cologne, 50829 Cologne, Germany
Plant Physiol 141:1293-305. 2006..This degradation is mediated by at least two domains indicating the presence of at least two different pathways impinging on ICK1/KRP1 protein stability... - Yama/CPP32 beta, a mammalian homolog of CED-3, is a CrmA-inhibitable protease that cleaves the death substrate poly(ADP-ribose) polymeraseM Tewari
Department of Pathology, University of Michigan Medical School, Ann Arbor 48109, USA
Cell 81:801-9. 1995..Furthermore, CrmA blocked cleavage of PARP in cells undergoing apoptosis. We propose that Yama may represent an effector component of the mammalian cell death pathway and suggest that CrmA blocks apoptosis by inhibiting Yama... - Effect of glycosylation on protein folding: a close look at thermodynamic stabilizationDalit Shental-Bechor
Department of Structural Biology, Weizmann Institute of Science, Rehovot 76100, Israel
Proc Natl Acad Sci U S A 105:8256-61. 2008.... - Follow the leader: the use of leader peptides to guide natural product biosynthesisTrent J Oman
Department of Chemistry, Howard Hughes Medical Institute, and Institute for Genomic Biology, University of Illinois at Urbana Champaign, Urbana, Illinois, USA
Nat Chem Biol 6:9-18. 2010.... - Lantibiotics: peptides of diverse structure and functionJoanne M Willey
Department of Biology, Hofstra University, Hempstead, New York 11549, USA
Annu Rev Microbiol 61:477-501. 2007.... - How important are post-translational modifications in p53 for selectivity in target-gene transcription and tumour suppression?A Olsson
Division of Developmental Immunology, Biocenter, Innsbruck Medical University, Innsbruck, Austria
Cell Death Differ 14:1561-75. 2007..Nevertheless, mouse genetics presents again its enormous power. Despite being relatively slow and tedious, it has become indispensable for researchers to sort out the wheat from the chaff in an endless sea of publications on p53... - Identification of a novel post-translational modification in Plasmodium falciparum: protein sumoylation in different cellular compartmentsNeha Issar
Institut Pasteur CNRS URA 2581, Biology of Host Parasite Interactions Unit, F75724 Paris, France
Cell Microbiol 10:1999-2011. 2008..falciparum sumoylated proteins. Our results imply that SUMO conjugation has an essential function in a number of different biological processes in P. falciparum... - PTMs on H3 variants before chromatin assembly potentiate their final epigenetic stateAlejandra Loyola
CNRS UMR 218, Institut Curie, Paris 75248, France
Mol Cell 24:309-16. 2006..Although the set of initial modifications present on H3.1 is permissive for further modifications, in H3.3 a subset cannot be K9me3. Thus, initial modifications impact final PTMs within chromatin... - Transcriptional regulation by the phosphorylation-dependent factor CREBB Mayr
Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, California 92037, USA
Nat Rev Mol Cell Biol 2:599-609. 2001..CREB is phosphorylated in response to various signals, but how is specificity achieved in these signalling pathways?.. - Posttranslational mechanisms regulate the mammalian circadian clockC Lee
Department of Neurobiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA
Cell 107:855-67. 2001..We also provide in vivo evidence that casein kinase I delta is a second clock relevant kinase... - Stressing the role of FoxO proteins in lifespan and diseaseArmando van der Horst
Department of Physiological Chemistry, Centre for Biomedical Genetics, University Medical Center Utrecht, 3584 CG Utrecht, The Netherlands
Nat Rev Mol Cell Biol 8:440-50. 2007..These regulatory enzymes affect FoxO and p53 function in an opposite manner. This shared yet opposing regulatory network between FoxOs and p53 may underlie a 'trade-off' between disease and lifespan... - Post-translational modification of p53 in tumorigenesisAnn M Bode
The Hormel Institute, University of Minnesota, 801 16th Avenue NE, Austin, Minnesota 55912, USA
Nat Rev Cancer 4:793-805. 2004..A thorough understanding of p53 will be extremely useful in the development of new strategies for treating and preventing cancer, including restoration of p53 function and selective killing of tumours with mutant TP53... - Regulation of the cell cycle at the G1-S transition by proteolysis of cyclin E and p27Kip1K I Nakayama
Department of Molecular and Cellular Biology, Laboratory of Embryonic and Genetic Engineering, Medical Institute of Bioregulation, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka, Fukuoka, 812 8582, Japan
Biochem Biophys Res Commun 282:853-60. 2001..In this article, recent progress in characterization of the molecular mechanisms that control the proteolysis of cyclin E and p27Kip1 is reviewed... - A metalloprotease-disintegrin, MDC9/meltrin-gamma/ADAM9 and PKCdelta are involved in TPA-induced ectodomain shedding of membrane-anchored heparin-binding EGF-like growth factorY Izumi
Department of Molecular Biology, Yokohama City University School of Medicine 3 9, Fuku ura, Kanagawa ku, Yokohama 236 0004, Japan
EMBO J 17:7260-72. 1998..These results suggest that MDC9 and PKCdelta are involved in the stimulus-coupled shedding of the proHB-EGF ectodomain... - MLL translocations, histone modifications and leukaemia stem-cell developmentAndrei V Krivtsov
Division of Haematology Oncology, Children s Hospital, Department of Pediatric Oncology, and Harvard Medical School, Boston, Massachusetts 02115, USA
Nat Rev Cancer 7:823-33. 2007..The link between a chromatin modulator and leukaemia stem cells provides support for epigenetic landscapes as an important part of leukaemia and normal stem-cell development... - Tools for analyzing and predicting N-terminal protein modificationsThierry Meinnel
Protein Maturation, Cell Fate and Therapeutics, Institut des Sciences du Vegetal, Centre National de la Recherche Scientifique, Gif sur Yvette, France
Proteomics 8:626-49. 2008..Progress is being made towards the annotation of databases containing information for complete proteomes, and should facilitate research into all areas of proteomics... - Gas2, a growth arrest-specific protein, is a component of the microfilament network systemC Brancolini
International Center for Genetic Engineering and Biotechnology I C G E B Trieste, Italy
J Cell Biol 117:1251-61. 1992..In none of the analyzed oncogene-transformed NIH 3T3 cell lines was Gas2 expression induced under serum starvation... - A proteomic analysis of arginine-methylated protein complexesFrancois Michel Boisvert
Terry Fox Molecular Oncology Group and Bloomfield Center for Research on Aging, Lady Davis Institute for Medical Research and Department of Oncology, McGill University, Montreal, Quebec H3T 1E2, Canada
Mol Cell Proteomics 2:1319-30. 2003..These findings provide a basis for the identification of the role of arginine methylation in many cellular processes... - Metadegradomics: toward in vivo quantitative degradomics of proteolytic post-translational modifications of the cancer proteomeAlain Doucet
Centre for Blood Research, 4 401 Life Sciences Institute, University of British Columbia, 2350 Health Sciences Mall, Vancouver, British Columbia V6T 1Z3, Canada
Mol Cell Proteomics 7:1925-51. 2008..At the global level, the N terminome analysis of whole communities of proteases in tissues and organs in vivo provides a full scale understanding of the protease web and the web-sculpted proteome, so defining metadegradomics... - Modulation of replication protein A function by its hyperphosphorylation-induced conformational change involving DNA binding domain BYiyong Liu
Department of Biochemistry and Molecular Biology, James H Quillen College of Medicine, East Tennessee State University, Johnson City, Tennessee 37614, USA
J Biol Chem 280:32775-83. 2005.... - Post-translational palmitoylation and glycosylation of Wnt-5a are necessary for its signallingManabu Kurayoshi
Department of Biochemistry, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima 734 8551, Japan
Biochem J 402:515-23. 2007..Thus the post-translational palmitoylation and glycosylation of Wnt-5a are important for the actions and secretion of Wnt-5a... - Reciprocal phosphorylation and glycosylation recognition motifs control NCAPP1 interaction with pumpkin phloem proteins and their cell-to-cell movementKen Ichiro Taoka
Section of Plant Biology, College of Biological Sciences, University of California, Davis, CA 95616, USA
Plant Cell 19:1866-84. 2007.... - Receptor specific downregulation of cytokine signaling by autophosphorylation in the FERM domain of Jak2Megumi Funakoshi-Tago
Department of Biochemistry, St Jude Children s Research Hospital, Memphis, TN 3810, USA
EMBO J 25:4763-72. 2006..This regulation occurs with the receptors for Epo, thrombopoietin and growth hormone but not with the receptor for interferon-gamma... - Tisp40, a spermatid specific bZip transcription factor, functions by binding to the unfolded protein response element via the Rip pathwayIppei Nagamori
Department of Molecular Genetics, Research Institute for Microbial Diseases, Osaka University, Yamadaoka 3 1, Suita City, Osaka, Japan
Genes Cells 10:575-94. 2005..These observations unveil a novel event in mouse spermiogenesis and show that the final stage of transcriptional regulation is controlled by the Rip pathway... - Tumor suppression by the von Hippel-Lindau protein requires phosphorylation of the acidic domainMartijn P Lolkema
Department of Medical Oncology, University Medical Center Utrecht, Heidelberglaan 100, 3584 CX Utrecht, The Netherlands
J Biol Chem 280:22205-11. 2005..We propose that phosphorylation of the acidic domain plays a role in the regulation of proper fibronectin matrix deposition and that this may be relevant for the development of VHL-associated malignancies...
Research Grants
- Glycoregulation of Skp1 in the cytoplasm and nucleusChristopher M West; Fiscal Year: 2011..gondii, which is widely disseminated latently in the human population and for which, if it is re-activated, pharmacological therapies are extremely limited. ..
- Glycoregulation of Skp1 in the cytoplasm and nucleusChristopher M West; Fiscal Year: 2010..gondii, which is widely disseminated latently in the human population and for which, if it is re-activated, pharmacological therapies are extremely limited. ..
- Glycoregulation of Skp1 in the cytoplasm and nucleusChristopher West; Fiscal Year: 2009..gondii, which is widely disseminated latently in the human population and for which, if it is re-activated, pharmacological therapies are extremely limited. ..
- The Effects of PP2A on TNF Signaling and Smoke-Induced Lung InjuryRobert F Foronjy; Fiscal Year: 2010..This proposal will advance public health by determining how PP2A, the primary eukaryotic serine/threonine phosphatase, alters the injurious responses to cigarette smoke exposure in the lung. ..
- Distance-Dependent Structure and Function of Neuronal DendritesKristen Harris; Fiscal Year: 2007....
- Distance-Dependent Structure and Function of Neuronal DendritesKristen Harris; Fiscal Year: 2009....
- Molecular Mechanisms of Histone AcetyltransferasesJohn M Denu; Fiscal Year: 2010..Several anti-cancer drugs have already been developed to target certain enzymes involved in this pathway. ..
- Molecular Mechanisms of Histone AcetyltransferasesJOHN DENU; Fiscal Year: 2009..Several anti-cancer drugs have already been developed to target certain enzymes involved in this pathway. ..
- MOLECULAR PHARMACOLOGY OF INSULIN RESISTANCE IN BURNSJEEVENDRA MARTYN; Fiscal Year: 2005..The studies together will thus provide significant insights into the pathogenesis of insulin resistance and provide information on novel therapeutic strategies to treat burn, and other stress or inflammation-induced insulin resistance. ..
- Bioflavonoid Effects on EGF Signaling and Cell Cycle Pathways in Prostate CancerBarry Markaverich; Fiscal Year: 2009..These studies may lead to the development of new drugs and/or dietary components for use in cancer prevention or treatment. ..
- Bioflavonoid Effects on EGF Signaling and Cell Cycle Pathways in Prostate CancerBARRY MATTHEW MARKAVERICH; Fiscal Year: 2010..These studies may lead to the development of new drugs and/or dietary components for use in cancer prevention or treatment. ..
- In vivo imaging of X inactivationMatthew Levy; Fiscal Year: 2010....
- Novel Mechanistic Targets of Steroid Hormones in the BrainMeharvan Singh; Fiscal Year: 2007....
- The functions of the US3 protein kinase of herpes simplex virusBernard Roizman; Fiscal Year: 2010....
- MODIFICATION OF ALPHA-CRYSTALLIN CHAPERONE FUNCTIONEDATHARA ABRAHAM; Fiscal Year: 2003..We will test the hypothesis that posttranslational modifications affect the chaperone-target protein binding leading to reduced chaperone function. ..
- Regulation of structure and function of protein by glycosylationZhiwen Jonathan Zhang; Fiscal Year: 2010....
- Regulation of structure and function of protein by glycosylationZhiwen Zhang; Fiscal Year: 2009....
- Inducible Nitric Oxide Synthase and Insulin ResistanceMasao Kaneki; Fiscal Year: 2004..abstract_text> ..
- Structual and Calcium Regulatory Proteins in SarcopeniaLaDora V Thompson; Fiscal Year: 2010..This proposal investigates two key skeletal muscle proteins, Troponin C and myosin light chains, which are candidates to explain age-related muscle dysfunction. ..
- Structual and Calcium Regulatory Proteins in SarcopeniaLaDora Thompson; Fiscal Year: 2009..This proposal investigates two key skeletal muscle proteins, Troponin C and myosin light chains, which are candidates to explain age-related muscle dysfunction. ..
- Enzymology of Post-translational ModificationsCarol A Fierke; Fiscal Year: 2010..Identification of the protein substrates and downstream biological function will enhance the development of new therapeutics targeting these pathways. ..
- CaaX Protein Processing and Human DiseaseStephen G Young; Fiscal Year: 2010..Our studies will be focused on the physiologic importance of the B-type lamins in the developing brain and other tissues and the physiologic importance of the posttranslational processing of prelamin A. ..
- Assays for Screening Histone Modifications in CancerMichael A Freitas; Fiscal Year: 2010....
- Biological role of modifications to C/EBPbeta-1 and -2Linda Sealy; Fiscal Year: 2007..These studies will provide important insights into how misregulated C/EBPbeta isoform expression may contribute to the development of metastatic mammary carcinoma. ..
- Structural Biology of Lysine Methylation in DNA Damage and Checkpoint SignalingGeorges Mer; Fiscal Year: 2010..The work has relevance to public health because by understanding the mechanisms of these important processes, we may be able to find ways to prevent and treat human malignancies, particularly cancer. ..
- Structure and Mechanism of Pathogen SET Domain HKMTsMing Ming Zhou; Fiscal Year: 2007....
- Structure and Mechanism of Pathogen SET Domain HKMTsMing Ming Zhou; Fiscal Year: 2009....
- The Epileptogenic Effect of Perinatal HypoxiaFrances Jensen; Fiscal Year: 2009..The present proposal aims to refine our understanding of this novel antiepileptogenic effect, and potentially elucidate other targets that can be used for therapeutic intervention in this unique population. ..
- The Epileptogenic Effect of Perinatal HypoxiaFrances E Jensen; Fiscal Year: 2010..The present proposal aims to refine our understanding of this novel antiepileptogenic effect, and potentially elucidate other targets that can be used for therapeutic intervention in this unique population. ..
- Glycoregulation of Skp1 in the cytoplasm and nucleusChristopher M West; Fiscal Year: 2010..The knowledge gained is expected to generate new ideas of how the proteome is regulated in select protists in response to external signals such as O2 and internal signals such as sugar metabolites. ..
- Novel Micro-X-ray Translucent Flow Cell Technology for Proteomics, a Broadly ApplEVA BIRNBAUM; Fiscal Year: 2007....
- Innate immune signal transduction specificity in inflammatory diseaseDerek W Abbott; Fiscal Year: 2011..This work aims to help determine the causes of inflammatory disease and aims to identify novel targets for pharmaceutical intervention in these debilitating disorders. ..
- Innate immune signal transduction specificity in inflammatory diseaseDerek W Abbott; Fiscal Year: 2010..This work aims to help determine the causes of inflammatory disease and aims to identify novel targets for pharmaceutical intervention in these debilitating disorders. ..
- Identifying and Characterizing Readers of the Neural Histone CodeMark Bedford; Fiscal Year: 2007..In this study we plan to investigate how this memory code is read. This basic scientific study will clearly impact the fields of drug addiction, mental retardation and aging diseases of the brain. ..
- De Novo Methyltransferase Function in Chromatin and CancerKeith D Robertson; Fiscal Year: 2010....
- Myocardial Ischemia-Reperfusion and Protein Phosphatase 2aPolly A Hofmann; Fiscal Year: 2010..the heart, can we improve the short and long term outcomes from ischemic events in the heart? Which of the unique PP2aB targeting subunits is key to improving post-ischemic recovery in the heart, and by what mechanisms does this occur? ..