genomic library

Summary

Summary: A form of GENE LIBRARY containing the complete DNA sequences present in the genome of a given organism. It contrasts with a cDNA library which contains only sequences utilized in protein coding (lacking introns).

Top Publications

  1. doi SNP discovery and allele frequency estimation by deep sequencing of reduced representation libraries
    Curtis P van Tassell
    Bovine Functional Genomics Laboratory, United States Department of Agriculture, Agricultural Research Service, 10300 Baltimore Avenue, Beltsville, Maryland 20705, USA
    Nat Methods 5:247-52. 2008
  2. pmc Genetic diversity of peanut (Arachis hypogaea L.) and its wild relatives based on the analysis of hypervariable regions of the genome
    Marcio de Carvalho Moretzsohn
    EMBRAPA Recursos Geneticos e Biotecnologia, C P 02372, 70 770 900, Brasilia DF, Brazil
    BMC Plant Biol 4:11. 2004
  3. pmc A linkage map for the B-genome of Arachis (Fabaceae) and its synteny to the A-genome
    Marcio C Moretzsohn
    EMBRAPA Recursos Geneticos e Biotecnologia, Brasilia, DF, Brazil
    BMC Plant Biol 9:40. 2009
  4. pmc Dynamics of genomic-library enrichment and identification of solvent tolerance genes for Clostridium acetobutylicum
    Jacob R Borden
    Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL 60208, USA
    Appl Environ Microbiol 73:3061-8. 2007
  5. ncbi Genome-wide RNAi screening in Caenorhabditis elegans
    Ravi S Kamath
    Wellcome Trust Cancer Research UK Institute and Department of Genetics, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK
    Methods 30:313-21. 2003
  6. pmc Functional characterization of the gene cluster from Pseudomonas syringae pv. phaseolicola NPS3121 involved in synthesis of phaseolotoxin
    Selene Aguilera
    CINVESTAV, IPN Unidad Irapuato, Departamento de Ingeniería Genética, Irapuato, Gto, Apdo Postal 629, CP 36500 Mexico
    J Bacteriol 189:2834-43. 2007
  7. ncbi A genomic library-based amplification approach (GL-PCR) for the mapping of multiple IS6110 insertion sites and strain differentiation of Mycobacterium tuberculosis
    Amine Namouchi
    Laboratory of Mycobacteria, Institut Pasteur de Tunis, 13, Place Pasteur, BP 74, 1002, Tunis Belvedre, Tunisie, Tunisia
    J Microbiol Methods 67:202-11. 2006
  8. pmc Metagenomic analyses of an uncultured viral community from human feces
    Mya Breitbart
    Department of Biology, San Diego State University, San Diego, California 92182, USA
    J Bacteriol 185:6220-3. 2003
  9. pmc Assessing pooled BAC and whole genome shotgun strategies for assembly of complex genomes
    Niina Haiminen
    IBM T J Watson Research Center, Yorktown Heights, NY 10598, USA
    BMC Genomics 12:194. 2011
  10. pmc Analysis of BAC end sequences in oak, a keystone forest tree species, providing insight into the composition of its genome
    Patricia Faivre Rampant
    INRA, URGV, Plant Genomics Research, F 91057 Evry, France
    BMC Genomics 12:292. 2011

Research Grants

  1. CHEMICAL PATHOLOGY OF NEUROLOGICAL DISORDERS
    Kunihiko Suzuki; Fiscal Year: 1999
  2. CHEMICAL PATHOLOGY OF NEUROLOGICAL DISORDERS
    Kunihiko Suzuki; Fiscal Year: 1993
  3. TREPONEMA PALLIDUM PATHOGENESIS ASSOCIATED MOLECULES
    Sheila Lukehart; Fiscal Year: 2001
  4. HUMAN GRANULOCYTIC EHRLICHIA
    JOHN DUMLER; Fiscal Year: 1999
  5. NUCLEAR ENCODED GENES REGULATE CHLOROPLAST TRANSLATION
    Amybeth Cohen; Fiscal Year: 2002
  6. B FORSYTHUS PROTEASE AND SIALIDASE--GENES AND SUBSTRATES
    MARK MAIDEN; Fiscal Year: 2003
  7. GENETIC DETERMINANTS OF OXYGEN TOXICITY
    Valeria Culotta; Fiscal Year: 1993
  8. MANNOPROTEIN BIOSYNTHESIS IN SACCHAROMYCES CEREVISIAE
    Annette Herscovics; Fiscal Year: 1991
  9. FUNCTIONAL ANALYSIS OF THE MYXOCOCCUS XANTHUS GENOME
    Philip Youderian; Fiscal Year: 2004
  10. CELLULAR MECHANISMS IN THE INDUCTION OF AROMATASE
    CAROLE MENDELSON; Fiscal Year: 1991

Detail Information

Publications217 found, 100 shown here

  1. doi SNP discovery and allele frequency estimation by deep sequencing of reduced representation libraries
    Curtis P van Tassell
    Bovine Functional Genomics Laboratory, United States Department of Agriculture, Agricultural Research Service, 10300 Baltimore Avenue, Beltsville, Maryland 20705, USA
    Nat Methods 5:247-52. 2008
    ..67. This approach for simultaneous de novo discovery of high-quality SNPs and population characterization of allele frequencies may be applied to any species with at least a partially sequenced genome...
  2. pmc Genetic diversity of peanut (Arachis hypogaea L.) and its wild relatives based on the analysis of hypervariable regions of the genome
    Marcio de Carvalho Moretzsohn
    EMBRAPA Recursos Geneticos e Biotecnologia, C P 02372, 70 770 900, Brasilia DF, Brazil
    BMC Plant Biol 4:11. 2004
    ..Here we report the use of a set of microsatellite markers, including newly developed ones, for phylogenetic inferences and the analysis of genetic variation of accessions of A. hypogea and its wild relatives...
  3. pmc A linkage map for the B-genome of Arachis (Fabaceae) and its synteny to the A-genome
    Marcio C Moretzsohn
    EMBRAPA Recursos Geneticos e Biotecnologia, Brasilia, DF, Brazil
    BMC Plant Biol 9:40. 2009
    ..This can be overcome by the utilization of wild species of Arachis, which present the A- and B-genomes in the diploid state, and show high levels of genetic variability...
  4. pmc Dynamics of genomic-library enrichment and identification of solvent tolerance genes for Clostridium acetobutylicum
    Jacob R Borden
    Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL 60208, USA
    Appl Environ Microbiol 73:3061-8. 2007
    A Clostridium acetobutylicum ATCC 824 genomic library was constructed using randomly sheared DNA. Library inserts conferring increased tolerance to 1-butanol were isolated using two protocols...
  5. ncbi Genome-wide RNAi screening in Caenorhabditis elegans
    Ravi S Kamath
    Wellcome Trust Cancer Research UK Institute and Department of Genetics, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK
    Methods 30:313-21. 2003
    ..We have used this library to perform genome-wide analyses of gene function in C. elegans. Here, we describe the protocols used for bacterial library construction and for high-throughput screening in C. elegans using RNAi by feeding...
  6. pmc Functional characterization of the gene cluster from Pseudomonas syringae pv. phaseolicola NPS3121 involved in synthesis of phaseolotoxin
    Selene Aguilera
    CINVESTAV, IPN Unidad Irapuato, Departamento de Ingeniería Genética, Irapuato, Gto, Apdo Postal 629, CP 36500 Mexico
    J Bacteriol 189:2834-43. 2007
    ..Preliminary results also indicate that the gene product of phtL is involved in the regulation of the synthesis of phaseolotoxin...
  7. ncbi A genomic library-based amplification approach (GL-PCR) for the mapping of multiple IS6110 insertion sites and strain differentiation of Mycobacterium tuberculosis
    Amine Namouchi
    Laboratory of Mycobacteria, Institut Pasteur de Tunis, 13, Place Pasteur, BP 74, 1002, Tunis Belvedre, Tunisie, Tunisia
    J Microbiol Methods 67:202-11. 2006
    ..strategy, referred to as GL-PCR, that amplifies IS6110-flanking sequences based on the construction of a genomic library. M...
  8. pmc Metagenomic analyses of an uncultured viral community from human feces
    Mya Breitbart
    Department of Biology, San Diego State University, San Diego, California 92182, USA
    J Bacteriol 185:6220-3. 2003
    ..Most of the sequences were unrelated to anything previously reported. The recognizable viruses were mostly siphophages, and the community contained an estimated 1,200 viral genotypes...
  9. pmc Assessing pooled BAC and whole genome shotgun strategies for assembly of complex genomes
    Niina Haiminen
    IBM T J Watson Research Center, Yorktown Heights, NY 10598, USA
    BMC Genomics 12:194. 2011
    ..We propose additional measures enabling easy and visual comparison of assembly quality, such as rearrangements and redundant sequence content, relative to the known target sequence...
  10. pmc Analysis of BAC end sequences in oak, a keystone forest tree species, providing insight into the composition of its genome
    Patricia Faivre Rampant
    INRA, URGV, Plant Genomics Research, F 91057 Evry, France
    BMC Genomics 12:292. 2011
    ..Deep-coverage large-insert genomic libraries are a crucial tool for attaining this objective. We report herein the construction of a BAC library for Quercus robur, its characterization and an analysis of BAC end sequences...
  11. ncbi Identification of a new gene encoding EPSPs with high glyphosate resistance from the metagenomic library
    Dan Jin
    College of Life Sciences, Key Laboratory of Bio resources and Eco environment, The Ministry of Education, Sichuan University, 610064, Chengdu, China
    Curr Microbiol 55:350-5. 2007
    ..A His-tagged RD fusion protein was produced into E. coli to characterize the enzymatic properties of the RD EPSP protein...
  12. ncbi Tall fescue genomic SSR markers: development and transferability across multiple grass species
    Malay C Saha
    Forage Improvement Division, The Samuel Roberts Noble Foundation, Inc, 2510 Sam Noble Parkway, Ardmore, OK 73401, USA
    Theor Appl Genet 113:1449-58. 2006
    ..and other forage grass species. A tall fescue genomic library enriched in (GA/CT)( n ) repeats was used to develop primer pairs (PPs) flanking SSRs and assess PP ..
  13. doi Identification of cellulase genes from the metagenomes of compost soils and functional characterization of one novel endoglucanase
    Hao Pang
    Guangxi Key Laboratory of Subtropical Bioresources Conservation and Utilization, College of Life Science and Technology, Guangxi University, Nanning, Guangxi, People s Republic of China
    Curr Microbiol 58:404-8. 2009
    ..The recombinant protein, Umcel9B, showed activity against carboxymethyl cellulose, indicating that Umcel9B is an endoactive enzyme. Enzymatic activity occurs optimally at a pH of 7.0 and a temperature of 25 degrees C...
  14. pmc Monitoring genomic sequences during SELEX using high-throughput sequencing: neutral SELEX
    Bob Zimmermann
    Max F Perutz Laboratories, Department of Biochemistry, University of Vienna, Vienna, Austria
    PLoS ONE 5:e9169. 2010
    ..Concerns have been raised regarding requirements imposed on RNA sequences undergoing SELEX selection...
  15. pmc Comprehensive analysis of MHC class I genes from the U-, S-, and Z-lineages in Atlantic salmon
    Morten F Lukacs
    Centre for Ecology and Evolutionary Synthesis, Dept of Biology, University of Oslo, Norway
    BMC Genomics 11:154. 2010
    ..A pseudolocus for Sasa-UCA was identified in the nonclassical IB region. Both regions contained genes for antigen presentation, as wells as orthologues to other genes residing in the human MHC region...
  16. ncbi Gene cloning and biochemical characterization of the BMP-2 of Pinctada fucata
    Tomoyuki Miyashita
    Department of Genetic Engineering, Faculty of Biology Oriented Science and Technology, Kinki University, Kinokawa, Wakayama, Japan
    Biosci Biotechnol Biochem 72:37-47. 2008
    ..The BMP-2 gene was expressed strongly in the inner part of the mantle tissue, corresponding to the nacreous aragonite shell layer. This finding suggests that BMP-2 has a key role in nacreous layer formation...
  17. doi Finding a (pine) needle in a haystack: chloroplast genome sequence divergence in rare and widespread pines
    J B Whittall
    Department of Biology, Santa Clara University, CA 95053, USA
    Mol Ecol 19:100-14. 2010
    ....
  18. pmc Genomic library screens for genes involved in n-butanol tolerance in Escherichia coli
    Luis H Reyes
    Department of Chemical Engineering, Texas A and M University, College Station, Texas, United States of America
    PLoS ONE 6:e17678. 2011
    ..However, the existing knowledge of genes involved in n-butanol tolerance is limited. The goal of this study is therefore to identify E. coli genes that are involved in n-butanol tolerance...
  19. pmc Gene discovery and gene function assignment in filamentous fungi
    L Hamer
    Paradigm Genetics, 108 Alexander Drive, Research Triangle Park, NC 27709, USA
    Proc Natl Acad Sci U S A 98:5110-5. 2001
    ..We further show that insertions can create alterations in gene expression, and we have used this approach to investigate an amino acid oxidation pathway in two important fungal phytopathogens...
  20. ncbi Construction of a genomic library of wild rice and Agrobacterium-mediated transformation of large insert DNA linked to BPH resistance locus
    Rui Feng He
    Key Laboratory of Ministry of Education for Plant Development Biology, College of Life Sciences, Wuhan University, Wuhan 430072, China
    Gene 321:113-21. 2003
    Here we report the first genomic library of wild rice constructed on a plant-transformation-competent binary vector (BIBAC2) and transformation of the large insert DNA into rice via Agrobacterium...
  21. ncbi Microsatellite identification and characterization in peanut ( A. hypogaea L.)
    M E Ferguson
    International Crops Research Institute for the Semi Arid Tropics ICRISAT, 502324, Patancheru, Andhra Pradesh, India
    Theor Appl Genet 108:1064-70. 2004
    ....
  22. ncbi The cellular immune recognition of proteins expressed by an African swine fever virus random genomic library
    J S Jenson
    Department of Immunology, Institute for Animal Health, Ash Road, Pirbright, Surrey GU24 ONF, UK
    J Immunol Methods 242:33-42. 2000
    The cellular immune recognition of peptides expressed by an African swine fever virus (ASFV) random genomic library has been studied...
  23. doi Genomic library screening for viruses from the human dental plaque revealed pathogen-specific lytic phage sequences
    Ahmed Nasser Al-Jarbou
    Department of Pharmaceutics, College of Pharmacy, Qassim University, Buraidah, Saudi Arabia
    Curr Microbiol 64:1-6. 2012
    ..These findings interestingly highlight the uniqueness of over half of the sequences, whilst the dominance of a pathogen-specific prophage sequences imply their role in virulence...
  24. doi The in vivo Down syndrome genomic library in mouse
    Yann Herault
    Institut de Genetique et de Biologie Moleculaire et Cellulaire, Translational medicine and Neurogenetics program, IGBMC, CNRS, INSERM, Universite de Strasbourg, UMR7104, UMR964, Illkirch, Strasbourg, France
    Prog Brain Res 197:169-97. 2012
    ..This review also addresses future research challenges relative to the making of new models and their combination to go further in the characterization of candidates and modifier of the DS features...
  25. ncbi Construction and application of a baculovirus genomic library
    Yin Chen
    Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, 310009, China
    Z Naturforsch C 64:574-80. 2009
    A random genomic library of the baculovirus Bombyx mori nucleopolyhedrovirus (BmNPV) was constructed and viral factors were identified by screening the regulator(s) for helicase gene expression...
  26. ncbi Construction and utility of 10-kb libraries for efficient clone-gap closure for rice genome sequencing
    Tae Jin Yang
    Arizona Genomics Institute, Department of Plant Sciences, 303 Forbes Building, University of Arizona, Tucson, AZ 85721, USA
    Theor Appl Genet 107:652-60. 2003
    ..This method represents a useful way to close clone gaps and thus to complete the entire rice genome...
  27. doi Identification of two novel esterases from a marine metagenomic library derived from South China Sea
    Xinmin Chu
    Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, Hefei, Anhui, 230027, China
    Appl Microbiol Biotechnol 80:615-25. 2008
    ..EstB displayed remarkable activity against p-nitrophenyl esters and was highly stable in 30% methanol, ethanol, dimethylformamide, and dimethyl sulfoxide, making EstB a potential candidate for industrial applications...
  28. ncbi Metagenomic analysis of coastal RNA virus communities
    Alexander I Culley
    Department of Botany, University of British Columbia, 3529 6270 University Boulevard, Vancouver, BC V6T 1Z4, Canada
    Science 312:1795-8. 2006
    ..Our results show that the oceans are a reservoir of previously unknown RNA viruses...
  29. ncbi Efficient large-scale development of microsatellites for marker and mapping applications in Brassica crop species
    A J Lowe
    IACR Long Ashton Research Station, Long Ashton, BS41 9AF, Bristol, UK
    Theor Appl Genet 108:1103-12. 2004
    ..napus mapping population. These 97 SSR markers were mapped relative to a framework of RFLP markers and detected 136 loci over all 19 linkage groups of the oilseed rape genome...
  30. pmc Identification of three wheat globulin genes by screening a Triticum aestivum BAC genomic library with cDNA from a diabetes-associated globulin
    Evelin Loit
    Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine, University of Ottawa, Ottawa, Canada
    BMC Plant Biol 9:93. 2009
    ..To investigate the genomic origin of the identified wheat protein cDNA, a hexaploid wheat genomic library from Glenlea cultivar was screened.
  31. pmc Isolation and sequence analysis of the wheat B genome subtelomeric DNA
    Elena A Salina
    Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Science, PR, Lavrentieva 10, Novosibirsk, 630090, Russia
    BMC Genomics 10:414. 2009
    ....
  32. ncbi Preliminary linkage map of the turkey (Meleagris gallopavo) based on microsatellite markers
    D W Burt
    Department of Genomics and Bioinformatics, Roslin Institute Edinburgh, Roslin, Midlothian, UK
    Anim Genet 34:399-409. 2003
    ..turkey genetic linkage map we decided to map new microsatellite sequences obtained from a GT-enriched turkey genomic library. In different chicken populations more than 35-55% of microsatellites are polymorphic...
  33. ncbi BAC library construction
    Kazutoyo Osoegawa
    BACPAC Resource Center, Children s Hospital Oakland Research Institute, CA, USA
    Methods Mol Biol 255:1-46. 2004
  34. pmc High-throughput bisulfite sequencing in mammalian genomes
    Zachary D Smith
    Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA 02138, USA
    Methods 48:226-32. 2009
    ..Contemporary sequencing and array-based technologies are compared against sample throughput and coverage, highlighting the variety of options available to investigate methylation on the genome-scale...
  35. ncbi Cloning and identification of novel cellulase genes from uncultured microorganisms in rabbit cecum and characterization of the expressed cellulases
    Yi Feng
    Guangxi Key Laboratory of Subtropical Bioresources Conservation and Utilization, The Key Laboratory of Ministry of Education for Microbial and Plant Genetic Engineering, Guangxi University, Nanning, Guangxi, 530005, People s Republic of China
    Appl Microbiol Biotechnol 75:319-28. 2007
    ..The enzymes cloned in this work represented at least some of the cellulases operating efficiently in the rabbit cecum. This work provides the first snapshot on the cellulases produced by bacteria in rabbit cecum...
  36. doi Development of SSR markers derived from SSR-enriched genomic library of eggplant (Solanum melongena L.)
    Tsukasa Nunome
    National Institute of Vegetable and Tea Science NIVTS, National Agriculture and Food Research Organization NARO, 360 Kusawa, Ano, Tsu, Mie 514 2392, Japan
    Theor Appl Genet 119:1143-53. 2009
    ..The linkage map spans a total length of 959.1 cM, with an average marker distance of 4.3 cM. The markers should be a useful resource for qualitative and quantitative trait mapping and for marker-assisted selection in eggplant breeding...
  37. pmc Conditionally amplifiable BACs: switching from single-copy to high-copy vectors and genomic clones
    Jadwiga Wild
    McArdle Laboratory for Cancer Research, University of Wisconsin Medical School, Madison, Wisconsin 53706, USA
    Genome Res 12:1434-44. 2002
    ..2% D-glucose to the growth medium helped toward the objective of obtaining a real SC state for all BAC systems, thus enhancing the stability of their maintenance, which became equivalent to cloning into the host chromosome..
  38. pmc Genome physical mapping from large-insert clones by fingerprint analysis with capillary electrophoresis: a robust physical map of Penicillium chrysogenum
    Zhanyou Xu
    Department of Soil and Crop Sciences, Texas A and M University College Station, TX 77843, USA
    Nucleic Acids Res 33:e50. 2005
    ....
  39. doi A new beta-galactosidase with a low temperature optimum isolated from the Antarctic Arthrobacter sp. 20B: gene cloning, purification and characterization
    Aneta Monika Białkowska
    Institute of Technical Biochemistry ITB, Technical University of Łódź, Stefanowskigo 4 10, 90 924 Łódź, Poland
    Arch Microbiol 191:825-35. 2009
    ....
  40. ncbi High frequency and large number of polymorphic microsatellites in cultured shrimp, Penaeus (Litopenaeus) vannamei [Crustacea:Decapoda]
    Dawn Meehan
    Environmental and Comparative Genomics Section, Department of Environmental and Population Health, Tufts University School of Veterinary Medicine, 200 Westboro Road, North Grafton, MA 01536, USA
    Mar Biotechnol (NY) 5:311-30. 2003
    A total of 1479 recombinant clones were obtained from a Sau3A-digested genomic library of Penaeus (Litopenaeus) vannamei and used for probe hybridization...
  41. doi In vitro selection of RNA aptamers derived from a genomic human library against the TAR RNA element of HIV-1
    Marguerite Watrin
    INSERM U869, European Institute of Chemistry and Biology, Pessac, France
    Biochemistry 48:6278-84. 2009
    ..The 5' ACCCAG loop of a1 constitutes a new motif of interaction with the TAR loop...
  42. pmc The Oryza bacterial artificial chromosome library resource: construction and analysis of 12 deep-coverage large-insert BAC libraries that represent the 10 genome types of the genus Oryza
    Jetty S S Ammiraju
    Arizona Genomics Institute, University of Arizona, Tucson, Arizona 85721 USA
    Genome Res 16:140-7. 2006
    ....
  43. ncbi Utilization of a zebra finch BAC library to determine the structure of an avian androgen receptor genomic region
    Meizhong Luo
    Department of Plant Sciences, Arizona Genomics Institute, University of Arizona, Tucson, AZ 85721, USA
    Genomics 87:181-90. 2006
    ..Comparison of BAC end sequences and the whole BAC sequence with the chicken genome draft sequence showed a high degree of conserved synteny between the zebra finch and the chicken genome...
  44. doi Identification of genes conferring arsenic resistance to Escherichia coli from an effluent treatment plant sludge metagenomic library
    Nar Singh Chauhan
    Microbial Genomics and Biotechnology Group, Institute of Genomics and Integrative Biology, Council of Scientific and Industrial Research CSIR, Delhi, India
    FEMS Microbiol Ecol 67:130-9. 2009
    ..ArsN alone resulted in an approximately sixfold higher resistance to sodium arsenate in wild-type Escherichia coli W3110...
  45. pmc An improved method to identify BAC clones using pooled overgos
    Kavitha Madishetty
    Department of Botany and Plant Sciences, University of California, Riverside, CA 92521 0124, USA
    Nucleic Acids Res 35:e5. 2007
    ..The resulting cost savings and reduced human exposure to radiation make the use of highly pooled overgo probes a more attractive approach for screening of BAC libraries from organisms with large genomes...
  46. pmc A genomic-library based discovery of a novel, possibly synthetic, acid-tolerance mechanism in Clostridium acetobutylicum involving non-coding RNAs and ribosomal RNA processing
    Jacob R Borden
    Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL 60208, USA
    Metab Eng 12:268-81. 2010
    We generated a genomic library from sheared Clostridium acetobutylicum ATCC 824 DNA, whereby inserts can be expressed in both directions from the thiolase promoter, P(thl). Serial transfer of library-bearing C...
  47. ncbi Updating the metagenomics toolbox
    Esther Gabor
    BRAIN Aktiengesellschaft, Zwingenberg, Germany
    Biotechnol J 2:201-6. 2007
    ..However, challenges still lie ahead. Most prominently, the efficient heterologous expression of a plethora of potentially interesting enzymes from unknown source organisms is not readily achieved...
  48. pmc DNA repair in Drosophila: insights from the Drosophila genome sequence
    J J Sekelsky
    Department of Biology, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    J Cell Biol 150:F31-6. 2000
  49. ncbi Complete genome of the mutualistic, N2-fixing grass endophyte Azoarcus sp. strain BH72
    Andrea Krause
    Laboratory of General Microbiology, University of Bremen, PO Box 330440, D 28334 Bremen, Germany
    Nat Biotechnol 24:1385-91. 2006
    ..The genome contains remarkably few mobile elements, indicating a low rate of recent gene transfer that is presumably due to adaptation to a stable, low-stress microenvironment...
  50. pmc Isolation and biochemical characterization of two novel metagenome-derived esterases
    C Elend
    Molekulare Enzymtechnologie, Universitat Duisburg Essen, Lotharstrasse 1, 47057 Duisburg, Germany
    Appl Environ Microbiol 72:3637-45. 2006
    ..The high level of stability of these enzymes together with their unique substrate specificities make them highly useful for biotechnological applications...
  51. pmc New softwares for automated microsatellite marker development
    Wellington Martins
    Department of Computer Science, Catholic University of Goias, Brazil
    Nucleic Acids Res 34:e31. 2006
    ..The software is freely available and simple to install in both Windows and Unix-based operating systems. Here we demonstrate the software by developing primer pairs for 427 new candidate markers for peanut...
  52. ncbi zA map for sequence analysis of the Arabidopsis thaliana genome
    M Marra
    Washington University Genome Sequencing Center, St Louis, Missouri 63108, USA
    Nat Genet 22:265-70. 1999
    ....
  53. pmc Genome-wide mapping of DNase hypersensitive sites using massively parallel signature sequencing (MPSS)
    Gregory E Crawford
    National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Genome Res 16:123-31. 2006
    ..This strategy, which can be applied to any cell line or tissue, will enable a better understanding of how chromatin structure dictates cell function and fate...
  54. pmc A highly redundant BAC library of Atlantic salmon (Salmo salar): an important tool for salmon projects
    Jim Thorsen
    Department of Basic Sciences and Aquatic Medicine, Section of Genetics, Norwegian School of Veterinary Science, P O Box 8146 Dep, N 0033 Oslo, Norway
    BMC Genomics 6:50. 2005
    ..Furthermore, large-scale mapping can utilize highly redundant libraries in genome projects, and hence provide valuable data on the genome structure...
  55. pmc Environmental genomics of "Haloquadratum walsbyi" in a saltern crystallizer indicates a large pool of accessory genes in an otherwise coherent species
    Boris A Legault
    Evolutionary Genomics Group, Division de Microbiologia, Universidad Miguel Hernandez, Apartado 18, San Juan 03550, Alicante, Spain
    BMC Genomics 7:171. 2006
    ..Similar studies carried out in other extreme environments have revealed very little diversity in gene content among the cell lineages present...
  56. pmc The first generation of a BAC-based physical map of Brassica rapa
    Jeong Hwan Mun
    Brassica Genomics Team, National Institute of Agricultural Biotechnology, Rural Development Administration, 225 Seodun dong, Gwonseon gu, Suwon 441 707, South Korea
    BMC Genomics 9:280. 2008
    ..Integration of a physical map with an existing genetic map by linking genetic markers and BAC clones in the sequencing pipeline provides a crucial resource for the ongoing genome sequencing effort and assembly of whole genome sequences...
  57. pmc Interactions of Escherichia coli RNA with bacteriophage MS2 coat protein: genomic SELEX
    T Shtatland
    Department of Molecular, University of Colorado, Boulder, CO 80309 0347, USA
    Nucleic Acids Res 28:E93. 2000
    ..Such methods may be useful tools for both randomized sequence SELEX and genomic SELEX...
  58. ncbi Lateral gene transfer and phylogenetic assignment of environmental fosmid clones
    Camilla L Nesbø
    Department of Biochemistry and Molecular Biology, Dalhousie University and Genome Atlantic, 5850 College Street, Halifax, Nova Scotia, Canada, B3H1X5
    Environ Microbiol 7:2011-26. 2005
    ..In several cases, we can infer co-transfer of functionally related genes, and generate hypotheses about mechanism and ecological significance of transfer...
  59. pmc Development of microsatellite markers from an enriched genomic library for genetic analysis of melon (Cucumis melo L.)
    Patricia Silva Ritschel
    Cellular Biology Department, IB University of Brasília UnB Campus Universitário, Asa Norte, CEP 70 910 900, Brasilia, DF, Brazil
    BMC Plant Biol 4:9. 2004
    ..Genomic microsatellite enriched libraries can be an efficient alternative for marker development in such species...
  60. pmc Utility of different gene enrichment approaches toward identifying and sequencing the maize gene space
    Nathan Michael Springer
    Center for Plant and Microbial Genomics, Department of Plant Biology, University of Minnesota, St Paul, Minnesota 55108, USA
    Plant Physiol 136:3023-33. 2004
    ..Analysis of the overlap of coverage and bias due to position within a gene suggest that RescueMu, methyl-filtration, and high-Cot methods are at least partially nonredundant...
  61. pmc Metagenomic profiling: microarray analysis of an environmental genomic library
    Jonathan L Sebat
    Environmental Research Institute, University of Idaho, Moscow, Idaho 83844 1052, USA
    Appl Environ Microbiol 69:4927-34. 2003
    ..Metagenomic profiling offers an effective approach for rapidly characterizing many clones and identifying the clones corresponding to unidentified species of microorganisms...
  62. ncbi Construction of a subgenomic BAC library specific for chromosomes 1D, 4D and 6D of hexaploid wheat
    Jaroslav Janda
    Laboratory of Molecular Cytogenetics and Cytometry, Institute of Experimental Botany, Sokolovska 6, 77200 Olomouc, Czech Republic
    Theor Appl Genet 109:1337-45. 2004
    ..Their availability should facilitate the analysis of wheat genome structure and evolution, development of cytogenetic maps, construction of local physical maps and map-based cloning of agronomically important genes...
  63. ncbi Multicopy suppressors for novel antibacterial compounds reveal targets and drug efflux susceptibility
    Xiaoming Li
    Department of Biochemistry, Antimicrobial Research Centre, McMaster University, 1200 Main Street West, Hamilton, Ontario L8N 3Z5, Canada
    Chem Biol 11:1423-30. 2004
    ..been exploited to select for genetic interactions between a particular mutant and clones from a random genomic library at high copy...
  64. pmc Boudicca, a retrovirus-like long terminal repeat retrotransposon from the genome of the human blood fluke Schistosoma mansoni
    Claudia S Copeland
    Department of Tropical Medicine, School of Public Health and Tropical Medicine, Tulane University Health Sciences Center, New Orleans, Louisiana 70112, USA
    J Virol 77:6153-66. 2003
    ..By reverse transcription-PCR, mRNA transcripts were detected in the sporocyst, cercaria, and adult developmental stages of S. mansoni, indicating that Boudicca is actively transcribed in this trematode...
  65. ncbi Construction and characterization of three BAC libraries for analysis of the chicken genome
    M K Lee
    Department of Soil and Crop Sciences, 2123 TAMUS, Texas A and M University, College Station, Texas 77843 2123, USA
    Anim Genet 34:151-2. 2003
  66. ncbi Rice genome organization: the centromere and genome interactions
    Nori Kurata
    Plant Genetics Laboratory, Genetic Strains Research Center, National Institute of Genetics, 1111 Yata, Mishima, Shizuoka 411 8540, Japan
    Ann Bot 90:427-35. 2002
    ..A genome-wide survey of interactive genetic loci has identified various reproductive barriers that may drive speciation of the rice genome. The significance of these findings in genome organization and evolution is discussed...
  67. pmc Integration of Cot analysis, DNA cloning, and high-throughput sequencing facilitates genome characterization and gene discovery
    Daniel G Peterson
    Center for Applied Genetic Technologies and Department of Crop and Soil Sciences, University of Georgia, Athens, Georgia 30602, USA
    Genome Res 12:795-807. 2002
    ..High-throughput sequencing of Cot clones may be a means of "capturing" the sequence complexity of eukaryotic genomes at unprecedented efficiency...
  68. ncbi Biomedical applications and studies of molecular evolution: a proposal for a primate genomic library resource
    Evan E Eichler
    Department of Genetics, Case Western Reserve University School of Medicine and University Hospitals of Cleveland, Cleveland, Ohio 44106, USA
    Genome Res 12:673-8. 2002
    ..Based on existing BAC library resources, we propose the construction of a panel of primate BAC libraries from phylogenetic anchor species for the purpose of comparative medicine as well as studies of genome evolution...
  69. pmc E-RNAi: a web application to design optimized RNAi constructs
    Zeynep Arziman
    Boveri Group Signaling and Functional Genomics, German Cancer Research Center, Im Neuenheimer Feld 580, D 69120 Heidelberg, Germany
    Nucleic Acids Res 33:W582-8. 2005
    ..We here describe a web-based tool to evaluate and design optimized dsRNA constructs. Moreover, the application also gives access to published predesigned dsRNAs. The E-RNAi web application is available at http://e-rnai.dkfz.de/...
  70. ncbi Development of a genomic library of near isogenic lines (NILs) in melon (Cucumis melo L.) from the exotic accession PI161375
    Iban Eduardo
    Departament de Genètica Vegetal, Laboratori de Genètica Molecular Vegetal CSIC IRTA, Carretera de Cabrils s n, 08348 Cabrils, Barcelona, Spain
    Theor Appl Genet 112:139-48. 2005
    ..The NILs can also be used as pre-competitive breeding lines in melon breeding projects...
  71. doi Isolation of a low-temperature adapted lipolytic enzyme from uncultivated micro-organism
    C Roh
    School of Chemical and Biological Engineering, Institute of Molecular Biology and Genetics, Seoul National University, Seoul, South Korea
    J Appl Microbiol 105:116-23. 2008
    ..The aim of the study was to isolate a novel lipolytic enzyme from the activated sludge of uncultured micro-organisms...
  72. pmc From raw materials to validated system: the construction of a genomic library and microarray to interpret systemic perturbations in Northern bobwhite
    Arun Rawat
    Department of Biological Sciences, University of Southern Mississippi, Hattiesburg, MS, USA
    Physiol Genomics 42:219-35. 2010
    ..Furthermore, the systemic impact of dinitrotoluenes on liver function appears conserved across species as PPAR signaling is similarly affected in fathead minnow liver tissue after exposure to 2,4-DNT...
  73. pmc Structural characterization of SIL, a gene frequently disrupted in T-cell acute lymphoblastic leukemia
    P D Aplan
    Naval Medical Branch, National Cancer Institute, Bethesda, Maryland 20889 5105
    Mol Cell Biol 11:5462-9. 1991
    ..The genomic structure of SIL was also analyzed. The gene consists of 18 exons distributed over 70 kb, with the 5' portion of the gene demonstrating alternate exon utilization...
  74. ncbi Toward closing rice telomere gaps: mapping and sequence characterization of rice subtelomere regions
    Tae Jin Yang
    Brassica Genomics Team, National Institute of Agricultural Biotechnology, RDA, Suwon, 441 707, Korea
    Theor Appl Genet 111:467-78. 2005
    ....
  75. doi Preparation of genome-wide DNA fragment libraries using bisulfite in polyacrylamide gel electrophoresis slices with formamide denaturation and quality control for massively parallel sequencing by oligonucleotide ligation and detection
    Swati S Ranade
    Applied Biosystems, Foster City, CA 94404, USA
    Anal Biochem 390:126-35. 2009
    ....
  76. pmc A BAC-based physical map of the Drosophila buzzatii genome
    Josefa González
    Departament de Genetica i de Microbiologia, Universitat Autonoma de Barcelona, 08193 Bellaterra Barcelona, Spain
    Genome Res 15:885-92. 2005
    ..We constructed a Bacterial Artificial Chromosome (BAC) genomic library of D. buzzatii using the shuttle vector pTARBAC2.1...
  77. pmc Bacterial artificial chromosome libraries for mouse sequencing and functional analysis
    K Osoegawa
    Department of Cancer Genetics, Roswell Park Cancer Institute, Buffalo, New York 14263 USA
    Genome Res 10:116-28. 2000
    ..The low rate of chimerism, approximately 1%, and the low level of detected rearrangements support the anticipated usefulness of the BAC libraries for genome research...
  78. ncbi High density of long dinucleotide microsatellites in Drosophila subobscura
    M Pascual
    Department of Molecular Biology and Genetics, Cornell University, New York, NY, USA
    Mol Biol Evol 17:1259-67. 2000
    ..subobscura compared with D. melanogaster are due to both a higher average effective population and a higher intrinsic slippage rate in the former species...
  79. pmc A census of rRNA genes and linked genomic sequences within a soil metagenomic library
    Mark R Liles
    Department of Plant Pathology Gaylord Nelson Institute of Environmental Studies, University of Wisconsin Madison, 1630 Linden Drive, Madison, WI 53706, USA
    Appl Environ Microbiol 69:2684-91. 2003
    ..This study is the first step in using genomics to reveal the physiology of as-yet-uncultured members of the Acidobacterium division...
  80. ncbi Molecular structure of a novel gypsy-Ty3-like retrotransposon (Kabuki) and nested retrotransposable elements on the W chromosome of the silkworm Bombyx mori
    H Abe
    Department of Biological Production, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Japan
    Mol Gen Genet 263:916-24. 2000
    ..Moreover, the Kabuki element is largely intact. These results suggest that many retrotransposable elements have accumulated on the W chromosome, and these elements are expected to evolve more slowly than those on other chromosomes...
  81. pmc FSY1, a novel gene encoding a specific fructose/H(+) symporter in the type strain of Saccharomyces carlsbergensis
    P Goncalves
    Centro de Recursos Microbiológicos, Biotechnology Unit, Faculty of Sciences and Technology, New University of Lisbon, 2825 114 Caparica, Portugal
    J Bacteriol 182:5628-30. 2000
    ..Fsy1p is only distantly related to the Hxt proteins that mediate facilitated diffusion of glucose and fructose in Saccharomyces cerevisiae and related species...
  82. doi Identification of immunogenic polypeptides from a Mycoplasma hyopneumoniae genome library by phage display
    Jonas Kügler
    Abteilung Biotechnologie, Institut für Biochemie und Biotechnologie, Technische Universitat Braunschweig, Braunschweig, Germany
    Appl Microbiol Biotechnol 80:447-58. 2008
    ..The fragmented genome of M. hyopneumoniae was cloned into a phage display vector, and the genomic library was packaged using the helperphage Hyperphage to enrich open reading frames (ORFs)...
  83. ncbi High-Cot sequence analysis of the maize genome
    Yinan Yuan
    Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907 1392, USA
    Plant J 34:249-55. 2003
    ....
  84. ncbi Broad host range vectors for stable genomic library construction
    Michael D Lynch
    Department of Chemical and Biological Engineering, UCB424 ECCH120, University of Colorado, Boulder, 80309, USA
    Biotechnol Bioeng 94:151-8. 2006
    We describe the construction of 36 stable vectors for genomic library construction in gram-negative species. These vectors contain the pBBR1 replicon that has been shown to stably replicate in every gram-negative species tested...
  85. ncbi Identification and characterisation of a melon genomic region containing a resistance gene cluster from a constructed BAC library. Microcolinearity between Cucumis melo and Arabidopsis thaliana
    Hans van Leeuwen
    Department de Genètica Molecular, Institut de Biologia Molecular de Barcelona, CID CSIC, Jordi Girona 18, 08034, Barcelona, Spain
    Plant Mol Biol 51:703-18. 2003
    ..The detailed analysis of this region provides a description of the gene structure and the presence of repetitive sequences in a defined fragment of the genome of Cucumis melo...
  86. pmc Parallel construction of orthologous sequence-ready clone contig maps in multiple species
    James W Thomas
    Genome Technology Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Genome Res 12:1277-85. 2002
    ..The described approach can be used to facilitate the multispecies comparative sequencing of targeted genomic regions and can be adapted for constructing BAC contig maps in other vertebrates...
  87. pmc Rainbow smelt (Osmerus mordax) genomic library and EST resources
    K R von Schalburg
    Centre for Biomedical Research, University of Victoria, Victoria, BC V8W 3N5, Canada
    Mar Biotechnol (NY) 10:487-91. 2008
    ..They also facilitate identification of genes important to fish and direct us toward new technologies for other studies in fish biology...
  88. ncbi Construction and characterization of a BAC library for the European sea bass Dicentrarchus labrax
    H A Whitaker
    Institute of Aquaculture, University of Stirling, Stirling FK9 4LA, UK
    Anim Genet 37:526. 2006
  89. pmc Characterization of a forest soil metagenome clone that confers indirubin and indigo production on Escherichia coli
    He Kyoung Lim
    Division of Applied Biology, College of Natural Resources and Life Science, Dong A University, Busan 604 714, Republic of Korea
    Appl Environ Microbiol 71:7768-77. 2005
    ..This finding suggests that these forest soil microbial genes are responsible for producing the pigment with antibacterial activity...
  90. ncbi Rhizobium meliloti homologs of Escherichia coli mur genes
    F Leach
    Department of Biology, Massachusetts Institute of Technology, Cambridge 02139
    Gene 148:87-90. 1994
    ..coli cell wall. The R. meliloti putative murD sequence is preceded by a partial ORF that shares sequence identity with mraY. The orientation of the two ORFs in R. meliloti is similar to that of the E. coli murD and mraY genes...
  91. pmc Identification and characterization of Lactobacillus helveticus PepO2, an endopeptidase with post-proline specificity
    Yo Shen Chen
    Department of Food Science, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    Appl Environ Microbiol 69:1276-82. 2003
    A post-proline endopeptidase (PepO2) was detected in cell extracts from a genomic library of Lactobacillus helveticus CNRZ32 by using the synthetic substrate N-acetyl-beta-casein-(f203-209)-rho-nitroanilide in a coupled reaction with ..
  92. ncbi The GLABRA2 gene encodes a homeo domain protein required for normal trichome development in Arabidopsis
    W G Rerie
    School of Biological Sciences, University of Nebraska Lincoln 68588 0118
    Genes Dev 8:1388-99. 1994
    ..This suggests that GL2 may regulate events required for the directional cell expansion observed during trichome formation...
  93. ncbi Structural analysis of a Lotus japonicus genome. V. Sequence features and mapping of sixty-four TAC clones which cover the 6.4 mb regions of the genome
    Tomohiko Kato
    Kazusa DNA Research Institute, 2 6 7 Kazusa Kamatari, Kisarazu, Chiba 292 0818, Japan
    DNA Res 10:277-85. 2003
    ..japonicus, MG-20 and Gifu B-129. The sequence data, gene information and mapping information are available through the World Wide Web at http://www.kazusa.or.jp/lotus/...
  94. ncbi Cloning and nucleotide sequencing of a 15 kb region of the Bacillus subtilis genome containing the iol operon
    K Yoshida
    Department of Biotechnology, Faculty of Engineering, Fukuyama University, Japan
    Microbiology 140:2289-98. 1994
    ..Based on the features of the nucleotide sequence determined and the results of the homology search, the primary structure of the iol operon is predicted...
  95. pmc Primary structure and functional expression of a Drosophila cyclic nucleotide-gated channel present in eyes and antennae
    A Baumann
    Institut für Biologische Informationsverarbeitung, Forschungszentrum Julich, Germany
    EMBO J 13:5040-50. 1994
    ..The channel mRNA is expressed in antennae and the visual system of Drosophila. It is proposed that CNG channels are involved in transduction cascades of both invertebrate photoreceptors and olfactory sensillae...
  96. ncbi Properties of Acinetobacter calcoaceticus recA and its contribution to intracellular gene conversion
    L A Gregg-Jolly
    Department of Biology, Yale University, New Haven, Connecticut 06511
    Mol Microbiol 12:985-92. 1994
    ..Strains that had acquired the mutant gene were sensitive to both MMS and u.v. light, were deficient in natural transformation, and failed to carry out catJ-dependent high-frequency repair of the pcaJ3125 mutation...
  97. pmc Sporulation and primary sigma factor homologous genes in Clostridium acetobutylicum
    U Sauer
    Institut fur Mikrobiologie, Georg August Universitat Gottingen, Germany
    J Bacteriol 176:6572-82. 1994
    ..The data presented here provide strong evidence that at least some of the complex regulation features of sporulation in B. subtilis are conserved in C. acetobutylicum and possibly Clostridium spp...
  98. ncbi Gene structure and cell type-specific expression of the human ATP synthase alpha subunit
    S Akiyama
    Department of Biochemistry, Jichi Medical School, Tochigi ken, Japan
    Biochim Biophys Acta 1219:129-40. 1994
    ..The enhancer activities derived from the 5'-deletion mutants of a hATP1-CAT chimeric gene were different in cell lines from four different human tissues, suggesting the existence of cell type-specific gene regulation...
  99. ncbi Characterization of a unique genomic clone located 5' upstream of the Oshsp16.9B gene on chromosome 1 in rice (Oryza sativa L. cv Tainung No. 67)
    J C Guan
    Department of Botany, National Taiwan University, 106 Taipei, Taiwan, R O C
    Theor Appl Genet 106:503-11. 2003
    ..A 1.6-kb DNA fragment was isolated from the EcoRI-digested rice genomic library probed with the cDNA pTS1 encoding a 16.9-kDa class-I sHSP...
  100. ncbi Analysis and toxic overexpression in Escherichia coli of a staphylococcal gene encoding isoleucyl-tRNA synthetase
    A F Chalker
    Department of Biotechnology, SmithKline Beecham Pharmaceuticals, Brockham Park, Surrey, UK
    Gene 141:103-8. 1994
    ..The gene was engineered for high-level expression in Escherichia coli. Ile-RS overproduction was toxic to the E. coli host, the magnitude of its observed effects being strain-dependent...
  101. ncbi Cloning and characterization of the SEC18 gene from Candida albicans
    A Nieto
    , , Burjassot, Valencia, Spain
    Yeast 9:875-87. 1993
    ..cerevisiae thermosensitive mutant using a C. albicans genomic library in YRp7...

Research Grants76

  1. CHEMICAL PATHOLOGY OF NEUROLOGICAL DISORDERS
    Kunihiko Suzuki; Fiscal Year: 1999
    ..Standard methodologies include cDNA and genomic library construction, screening, the polymerase chain reaction, sequencing, allele-specific oligonucleotide screening,..
  2. CHEMICAL PATHOLOGY OF NEUROLOGICAL DISORDERS
    Kunihiko Suzuki; Fiscal Year: 1993
    ..Standard methodologies include cDNA and genomic library construction, screening, the polymerase chain reaction, sequencing, allele-specific oligonucleotide screening,..
  3. TREPONEMA PALLIDUM PATHOGENESIS ASSOCIATED MOLECULES
    Sheila Lukehart; Fiscal Year: 2001
    ..and homology with known virulence factors, and full-length genes for each ORF will be obtained from a genomic library or by PCR amplification of the ORF using primer sequences from the T...
  4. HUMAN GRANULOCYTIC EHRLICHIA
    JOHN DUMLER; Fiscal Year: 1999
    ..Genes encoding immunodominant, surface-exposed proteins of HGE will be cloned from a genomic library and sequenced. The sequences will be compared with homologs in E. equi and other ehrlichiae. 3...
  5. NUCLEAR ENCODED GENES REGULATE CHLOROPLAST TRANSLATION
    Amybeth Cohen; Fiscal Year: 2002
    ..We have produced antisera against these proteins, and have isolated two of the genomic clones: a screen of a genomic library should allow isolation of the third clone...
  6. B FORSYTHUS PROTEASE AND SIALIDASE--GENES AND SUBSTRATES
    MARK MAIDEN; Fiscal Year: 2003
    ..The experimental design will be to first isolate clones of the two genes by screening a B. forsythus genomic library (Specific Aims 1 and 3). The cloned genes will be sequences and expressed in E. coli...
  7. GENETIC DETERMINANTS OF OXYGEN TOXICITY
    Valeria Culotta; Fiscal Year: 1993
    ..to meet these goals are as follows: (AIM 1) To clone the BSD2 gene: This gene will be isolated from a yeast genomic library through functional complementation...
  8. MANNOPROTEIN BIOSYNTHESIS IN SACCHAROMYCES CEREVISIAE
    Annette Herscovics; Fiscal Year: 1991
    ..amino acid sequencing in order to clone the gene encoding this processing enzyme by screening a lambda gt11 genomic library with the antibodies and with synthetic oligonucleotides...
  9. FUNCTIONAL ANALYSIS OF THE MYXOCOCCUS XANTHUS GENOME
    Philip Youderian; Fiscal Year: 2004
    ..More important, these P22+insert particles are powerful genetic elements that allow us to order a genomic library of M. xanthus inserts by the functional approach of complementation of Salmonella auxotrophies...
  10. CELLULAR MECHANISMS IN THE INDUCTION OF AROMATASE
    CAROLE MENDELSON; Fiscal Year: 1991
    ..The cDNAs will be used to screen a human genomic library for isolation of the P-450AROM gene, which will be characterized and partially sequenced...