genomic library


Summary: A form of GENE LIBRARY containing the complete DNA sequences present in the genome of a given organism. It contrasts with a cDNA library which contains only sequences utilized in protein coding (lacking introns).

Top Publications

  1. ncbi SNP discovery and allele frequency estimation by deep sequencing of reduced representation libraries
    Curtis P van Tassell
    Bovine Functional Genomics Laboratory, United States Department of Agriculture, Agricultural Research Service, 10300 Baltimore Avenue, Beltsville, Maryland 20705, USA
    Nat Methods 5:247-52. 2008
  2. pmc Complex landscapes of somatic rearrangement in human breast cancer genomes
    Philip J Stephens
    Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1SA, UK
    Nature 462:1005-10. 2009
  3. pmc Genetic diversity of peanut (Arachis hypogaea L.) and its wild relatives based on the analysis of hypervariable regions of the genome
    Marcio de Carvalho Moretzsohn
    EMBRAPA Recursos Geneticos e Biotecnologia, C P 02372, 70 770 900, Brasilia DF, Brazil
    BMC Plant Biol 4:11. 2004
  4. pmc Genomic library screens for genes involved in n-butanol tolerance in Escherichia coli
    Luis H Reyes
    Department of Chemical Engineering, Texas A and M University, College Station, Texas, United States of America
    PLoS ONE 6:e17678. 2011
  5. ncbi Enzymes involved in the biogenesis of the nematode cuticle
    Antony P Page
    Wellcome Centre for Molecular Parasitology, The Anderson College, The University of Glasgow, Glasgow G11 6NU, UK
    Adv Parasitol 53:85-148. 2003
  6. ncbi Genomic library screening for viruses from the human dental plaque revealed pathogen-specific lytic phage sequences
    Ahmed Nasser Al-Jarbou
    Department of Pharmaceutics, College of Pharmacy, Qassim University, Buraidah, Saudi Arabia
    Curr Microbiol 64:1-6. 2012
  7. pmc Comprehensive analysis of MHC class I genes from the U-, S-, and Z-lineages in Atlantic salmon
    Morten F Lukacs
    Centre for Ecology and Evolutionary Synthesis, Dept of Biology, University of Oslo, Norway
    BMC Genomics 11:154. 2010
  8. pmc Monitoring genomic sequences during SELEX using high-throughput sequencing: neutral SELEX
    Bob Zimmermann
    Max F Perutz Laboratories, Department of Biochemistry, University of Vienna, Vienna, Austria
    PLoS ONE 5:e9169. 2010
  9. pmc Metagenomic islands of hyperhalophiles: the case of Salinibacter ruber
    Lejla Pasic
    Evolutionary Genomics Group, Division de Microbiologia, Universidad Miguel Hernandez, Apartado 18, San Juan 03550, Alicante, Spain
    BMC Genomics 10:570. 2009
  10. ncbi Genome-wide RNAi screening in Caenorhabditis elegans
    Ravi S Kamath
    Wellcome Trust Cancer Research UK Institute and Department of Genetics, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK
    Methods 30:313-21. 2003

Detail Information

Publications234 found, 100 shown here

  1. ncbi SNP discovery and allele frequency estimation by deep sequencing of reduced representation libraries
    Curtis P van Tassell
    Bovine Functional Genomics Laboratory, United States Department of Agriculture, Agricultural Research Service, 10300 Baltimore Avenue, Beltsville, Maryland 20705, USA
    Nat Methods 5:247-52. 2008
    ..67. This approach for simultaneous de novo discovery of high-quality SNPs and population characterization of allele frequencies may be applied to any species with at least a partially sequenced genome...
  2. pmc Complex landscapes of somatic rearrangement in human breast cancer genomes
    Philip J Stephens
    Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1SA, UK
    Nature 462:1005-10. 2009
    ..The study provides a new perspective on cancer genomes, highlighting the diversity of somatic rearrangements and their potential contribution to cancer development...
  3. pmc Genetic diversity of peanut (Arachis hypogaea L.) and its wild relatives based on the analysis of hypervariable regions of the genome
    Marcio de Carvalho Moretzsohn
    EMBRAPA Recursos Geneticos e Biotecnologia, C P 02372, 70 770 900, Brasilia DF, Brazil
    BMC Plant Biol 4:11. 2004
    ..Here we report the use of a set of microsatellite markers, including newly developed ones, for phylogenetic inferences and the analysis of genetic variation of accessions of A. hypogea and its wild relatives...
  4. pmc Genomic library screens for genes involved in n-butanol tolerance in Escherichia coli
    Luis H Reyes
    Department of Chemical Engineering, Texas A and M University, College Station, Texas, United States of America
    PLoS ONE 6:e17678. 2011
    ..However, the existing knowledge of genes involved in n-butanol tolerance is limited. The goal of this study is therefore to identify E. coli genes that are involved in n-butanol tolerance...
  5. ncbi Enzymes involved in the biogenesis of the nematode cuticle
    Antony P Page
    Wellcome Centre for Molecular Parasitology, The Anderson College, The University of Glasgow, Glasgow G11 6NU, UK
    Adv Parasitol 53:85-148. 2003
    ..This model system will be the main focus of this review...
  6. ncbi Genomic library screening for viruses from the human dental plaque revealed pathogen-specific lytic phage sequences
    Ahmed Nasser Al-Jarbou
    Department of Pharmaceutics, College of Pharmacy, Qassim University, Buraidah, Saudi Arabia
    Curr Microbiol 64:1-6. 2012
    ..These findings interestingly highlight the uniqueness of over half of the sequences, whilst the dominance of a pathogen-specific prophage sequences imply their role in virulence...
  7. pmc Comprehensive analysis of MHC class I genes from the U-, S-, and Z-lineages in Atlantic salmon
    Morten F Lukacs
    Centre for Ecology and Evolutionary Synthesis, Dept of Biology, University of Oslo, Norway
    BMC Genomics 11:154. 2010
    ..A pseudolocus for Sasa-UCA was identified in the nonclassical IB region. Both regions contained genes for antigen presentation, as wells as orthologues to other genes residing in the human MHC region...
  8. pmc Monitoring genomic sequences during SELEX using high-throughput sequencing: neutral SELEX
    Bob Zimmermann
    Max F Perutz Laboratories, Department of Biochemistry, University of Vienna, Vienna, Austria
    PLoS ONE 5:e9169. 2010
    ..Concerns have been raised regarding requirements imposed on RNA sequences undergoing SELEX selection...
  9. pmc Metagenomic islands of hyperhalophiles: the case of Salinibacter ruber
    Lejla Pasic
    Evolutionary Genomics Group, Division de Microbiologia, Universidad Miguel Hernandez, Apartado 18, San Juan 03550, Alicante, Spain
    BMC Genomics 10:570. 2009
    ..This kind of analysis reveals the presence of metagenomic islands, i.e. highly variable regions among the different lineages in the population...
  10. ncbi Genome-wide RNAi screening in Caenorhabditis elegans
    Ravi S Kamath
    Wellcome Trust Cancer Research UK Institute and Department of Genetics, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK
    Methods 30:313-21. 2003
    ..We have used this library to perform genome-wide analyses of gene function in C. elegans. Here, we describe the protocols used for bacterial library construction and for high-throughput screening in C. elegans using RNAi by feeding...
  11. ncbi Finding a (pine) needle in a haystack: chloroplast genome sequence divergence in rare and widespread pines
    J B Whittall
    Department of Biology, Santa Clara University, CA 95053, USA
    Mol Ecol 19:100-14. 2010
  12. pmc Functional characterization of the gene cluster from Pseudomonas syringae pv. phaseolicola NPS3121 involved in synthesis of phaseolotoxin
    Selene Aguilera
    CINVESTAV, IPN Unidad Irapuato, Departamento de Ingeniería Genética, Irapuato, Gto, Apdo Postal 629, CP 36500 Mexico
    J Bacteriol 189:2834-43. 2007
    ..Preliminary results also indicate that the gene product of phtL is involved in the regulation of the synthesis of phaseolotoxin...
  13. pmc Circumventing heterozygosity: sequencing the amplified genome of a single haploid Drosophila melanogaster embryo
    Charles H Langley
    Department of Evolution and Ecology, University of California, Davis, California 95616 8554, USA
    Genetics 188:239-46. 2011
    ..This method provides a solid path forward for population genomic sequencing and offers applications to many other systems in which small amounts of genomic DNA have unique experimental relevance...
  14. pmc Genome-wide mapping of DNase hypersensitive sites using massively parallel signature sequencing (MPSS)
    Gregory E Crawford
    National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Genome Res 16:123-31. 2006
    ..This strategy, which can be applied to any cell line or tissue, will enable a better understanding of how chromatin structure dictates cell function and fate...
  15. ncbi Diversity of microbes associated with the marine sponge, Haliclona simulans, isolated from Irish waters and identification of polyketide synthase genes from the sponge metagenome
    Jonathan Kennedy
    Environmental Research Institute, University College Cork, Cork, Ireland
    Environ Microbiol 10:1888-902. 2008
    ..Analyses of these clones revealed a diverse collection of PKS sequences which were most closely affiliated with PKS from members of the Cyanobacteria, Myxobacteria and Dinoflagellata...
  16. ncbi The in vivo Down syndrome genomic library in mouse
    Yann Herault
    Institut de Genetique et de Biologie Moleculaire et Cellulaire, Translational medicine and Neurogenetics program, IGBMC, CNRS, INSERM, Universite de Strasbourg, UMR7104, UMR964, Illkirch, Strasbourg, France
    Prog Brain Res 197:169-97. 2012
    ..This review also addresses future research challenges relative to the making of new models and their combination to go further in the characterization of candidates and modifier of the DS features...
  17. ncbi SCALEs: multiscale analysis of library enrichment
    Michael D Lynch
    Department of Chemical and Biological Engineering, University of Colorado, ECCH 111, Campus Box 424, Boulder, Colorado 80309, USA
    Nat Methods 4:87-93. 2007
    ..approach allows for identification of all single open reading frames and larger multigene fragments within a genomic library that alter the expression of a given phenotype...
  18. pmc Analysis of BAC end sequences in oak, a keystone forest tree species, providing insight into the composition of its genome
    Patricia Faivre Rampant
    INRA, URGV, Plant Genomics Research, F 91057 Evry, France
    BMC Genomics 12:292. 2011
    ..Deep-coverage large-insert genomic libraries are a crucial tool for attaining this objective. We report herein the construction of a BAC library for Quercus robur, its characterization and an analysis of BAC end sequences...
  19. pmc Metagenomic analyses of an uncultured viral community from human feces
    Mya Breitbart
    Department of Biology, San Diego State University, San Diego, California 92182, USA
    J Bacteriol 185:6220-3. 2003
    ..Most of the sequences were unrelated to anything previously reported. The recognizable viruses were mostly siphophages, and the community contained an estimated 1,200 viral genotypes...
  20. pmc Assessing pooled BAC and whole genome shotgun strategies for assembly of complex genomes
    Niina Haiminen
    IBM T J Watson Research Center, Yorktown Heights, NY 10598, USA
    BMC Genomics 12:194. 2011
    ..We propose additional measures enabling easy and visual comparison of assembly quality, such as rearrangements and redundant sequence content, relative to the known target sequence...
  21. pmc DNA repair in Drosophila: insights from the Drosophila genome sequence
    J J Sekelsky
    Department of Biology, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    J Cell Biol 150:F31-6. 2000
  22. ncbi Development of SSR markers derived from SSR-enriched genomic library of eggplant (Solanum melongena L.)
    Tsukasa Nunome
    National Institute of Vegetable and Tea Science NIVTS, National Agriculture and Food Research Organization NARO, 360 Kusawa, Ano, Tsu, Mie 514 2392, Japan
    Theor Appl Genet 119:1143-53. 2009
    ..The linkage map spans a total length of 959.1 cM, with an average marker distance of 4.3 cM. The markers should be a useful resource for qualitative and quantitative trait mapping and for marker-assisted selection in eggplant breeding...
  23. ncbi Identification of cellulase genes from the metagenomes of compost soils and functional characterization of one novel endoglucanase
    Hao Pang
    Guangxi Key Laboratory of Subtropical Bioresources Conservation and Utilization, College of Life Science and Technology, Guangxi University, Nanning, Guangxi, People s Republic of China
    Curr Microbiol 58:404-8. 2009
    ..The recombinant protein, Umcel9B, showed activity against carboxymethyl cellulose, indicating that Umcel9B is an endoactive enzyme. Enzymatic activity occurs optimally at a pH of 7.0 and a temperature of 25 degrees C...
  24. ncbi Construction and characterization of a BAC library for the European sea bass Dicentrarchus labrax
    H A Whitaker
    Institute of Aquaculture, University of Stirling, Stirling FK9 4LA, UK
    Anim Genet 37:526. 2006
  25. pmc Isolation and sequence analysis of the wheat B genome subtelomeric DNA
    Elena A Salina
    Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Science, PR, Lavrentieva 10, Novosibirsk, 630090, Russia
    BMC Genomics 10:414. 2009
  26. ncbi High frequency and large number of polymorphic microsatellites in cultured shrimp, Penaeus (Litopenaeus) vannamei [Crustacea:Decapoda]
    Dawn Meehan
    Environmental and Comparative Genomics Section, Department of Environmental and Population Health, Tufts University School of Veterinary Medicine, 200 Westboro Road, North Grafton, MA 01536, USA
    Mar Biotechnol (NY) 5:311-30. 2003
    A total of 1479 recombinant clones were obtained from a Sau3A-digested genomic library of Penaeus (Litopenaeus) vannamei and used for probe hybridization...
  27. pmc Gene discovery and gene function assignment in filamentous fungi
    L Hamer
    Paradigm Genetics, 108 Alexander Drive, Research Triangle Park, NC 27709, USA
    Proc Natl Acad Sci U S A 98:5110-5. 2001
    ..We further show that insertions can create alterations in gene expression, and we have used this approach to investigate an amino acid oxidation pathway in two important fungal phytopathogens...
  28. ncbi Multicopy suppressors for novel antibacterial compounds reveal targets and drug efflux susceptibility
    Xiaoming Li
    Department of Biochemistry, Antimicrobial Research Centre, McMaster University, 1200 Main Street West, Hamilton, Ontario L8N 3Z5, Canada
    Chem Biol 11:1423-30. 2004
    ..been exploited to select for genetic interactions between a particular mutant and clones from a random genomic library at high copy...
  29. ncbi Construction of a genomic library of the food spoilage yeast Zygosaccharomyces bailii and isolation of the beta-isopropylmalate dehydrogenase gene (ZbLEU2)
    F Rodrigues
    , Departamento de Biologia, Universidad do Minho, Campus de Gualtar, Braga, Portugal
    FEMS Yeast Res 1:67-71. 2001
    A genomic library of the yeast Zygosaccharomyces bailii ISA 1307 was constructed in pRS316, a shuttle vector for Saccharomyces cerevisiae and Escherichia coli...
  30. ncbi Complete genome of the mutualistic, N2-fixing grass endophyte Azoarcus sp. strain BH72
    Andrea Krause
    Laboratory of General Microbiology, University of Bremen, PO Box 330440, D 28334 Bremen, Germany
    Nat Biotechnol 24:1385-91. 2006
    ..The genome contains remarkably few mobile elements, indicating a low rate of recent gene transfer that is presumably due to adaptation to a stable, low-stress microenvironment...
  31. ncbi Identification of two novel esterases from a marine metagenomic library derived from South China Sea
    Xinmin Chu
    Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, Hefei, Anhui, 230027, China
    Appl Microbiol Biotechnol 80:615-25. 2008
    ..EstB displayed remarkable activity against p-nitrophenyl esters and was highly stable in 30% methanol, ethanol, dimethylformamide, and dimethyl sulfoxide, making EstB a potential candidate for industrial applications...
  32. ncbi Construction and utility of 10-kb libraries for efficient clone-gap closure for rice genome sequencing
    Tae Jin Yang
    Arizona Genomics Institute, Department of Plant Sciences, 303 Forbes Building, University of Arizona, Tucson, AZ 85721, USA
    Theor Appl Genet 107:652-60. 2003
    ..This method represents a useful way to close clone gaps and thus to complete the entire rice genome...
  33. ncbi Construction and application of a baculovirus genomic library
    Yin Chen
    Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, 310009, China
    Z Naturforsch C 64:574-80. 2009
    A random genomic library of the baculovirus Bombyx mori nucleopolyhedrovirus (BmNPV) was constructed and viral factors were identified by screening the regulator(s) for helicase gene expression...
  34. ncbi The cellular immune recognition of proteins expressed by an African swine fever virus random genomic library
    J S Jenson
    Department of Immunology, Institute for Animal Health, Ash Road, Pirbright, Surrey GU24 ONF, UK
    J Immunol Methods 242:33-42. 2000
    The cellular immune recognition of peptides expressed by an African swine fever virus (ASFV) random genomic library has been studied...
  35. ncbi Microsatellite identification and characterization in peanut ( A. hypogaea L.)
    M E Ferguson
    International Crops Research Institute for the Semi Arid Tropics ICRISAT, 502324, Patancheru, Andhra Pradesh, India
    Theor Appl Genet 108:1064-70. 2004
  36. ncbi Construction of a genomic library of wild rice and Agrobacterium-mediated transformation of large insert DNA linked to BPH resistance locus
    Rui Feng He
    Key Laboratory of Ministry of Education for Plant Development Biology, College of Life Sciences, Wuhan University, Wuhan 430072, China
    Gene 321:113-21. 2003
    Here we report the first genomic library of wild rice constructed on a plant-transformation-competent binary vector (BIBAC2) and transformation of the large insert DNA into rice via Agrobacterium...
  37. ncbi Efficient large-scale development of microsatellites for marker and mapping applications in Brassica crop species
    A J Lowe
    IACR Long Ashton Research Station, Long Ashton, BS41 9AF, Bristol, UK
    Theor Appl Genet 108:1103-12. 2004
    ..napus mapping population. These 97 SSR markers were mapped relative to a framework of RFLP markers and detected 136 loci over all 19 linkage groups of the oilseed rape genome...
  38. ncbi Metagenomic analysis of coastal RNA virus communities
    Alexander I Culley
    Department of Botany, University of British Columbia, 3529 6270 University Boulevard, Vancouver, BC V6T 1Z4, Canada
    Science 312:1795-8. 2006
    ..Our results show that the oceans are a reservoir of previously unknown RNA viruses...
  39. ncbi BAC library construction
    Kazutoyo Osoegawa
    BACPAC Resource Center, Children s Hospital Oakland Research Institute, CA, USA
    Methods Mol Biol 255:1-46. 2004
  40. pmc Identification of three wheat globulin genes by screening a Triticum aestivum BAC genomic library with cDNA from a diabetes-associated globulin
    Evelin Loit
    Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine, University of Ottawa, Ottawa, Canada
    BMC Plant Biol 9:93. 2009
    ..To investigate the genomic origin of the identified wheat protein cDNA, a hexaploid wheat genomic library from Glenlea cultivar was screened.
  41. ncbi Maize genome sequencing by methylation filtration
    Lance E Palmer
    Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
    Science 302:2115-7. 2003
    ..Reverse transcription polymerase chain reaction can be used to finish the maize transcriptome...
  42. pmc High-throughput bisulfite sequencing in mammalian genomes
    Zachary D Smith
    Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA 02138, USA
    Methods 48:226-32. 2009
    ..Contemporary sequencing and array-based technologies are compared against sample throughput and coverage, highlighting the variety of options available to investigate methylation on the genome-scale...
  43. pmc Conditionally amplifiable BACs: switching from single-copy to high-copy vectors and genomic clones
    Jadwiga Wild
    McArdle Laboratory for Cancer Research, University of Wisconsin Medical School, Madison, Wisconsin 53706, USA
    Genome Res 12:1434-44. 2002
    ..2% D-glucose to the growth medium helped toward the objective of obtaining a real SC state for all BAC systems, thus enhancing the stability of their maintenance, which became equivalent to cloning into the host chromosome..
  44. ncbi Cloning and identification of novel cellulase genes from uncultured microorganisms in rabbit cecum and characterization of the expressed cellulases
    Yi Feng
    Guangxi Key Laboratory of Subtropical Bioresources Conservation and Utilization, The Key Laboratory of Ministry of Education for Microbial and Plant Genetic Engineering, Guangxi University, Nanning, Guangxi, 530005, People s Republic of China
    Appl Microbiol Biotechnol 75:319-28. 2007
    ..The enzymes cloned in this work represented at least some of the cellulases operating efficiently in the rabbit cecum. This work provides the first snapshot on the cellulases produced by bacteria in rabbit cecum...
  45. ncbi A genomic library-based amplification approach (GL-PCR) for the mapping of multiple IS6110 insertion sites and strain differentiation of Mycobacterium tuberculosis
    Amine Namouchi
    Laboratory of Mycobacteria, Institut Pasteur de Tunis, 13, Place Pasteur, BP 74, 1002, Tunis Belvedre, Tunisie, Tunisia
    J Microbiol Methods 67:202-11. 2006
    ..strategy, referred to as GL-PCR, that amplifies IS6110-flanking sequences based on the construction of a genomic library. M...
  46. pmc Libraries for genomic SELEX
    B S Singer
    Department of Molecular Biology, University of Colorado, Boulder 80309 0347, USA
    Nucleic Acids Res 25:781-6. 1997
    ..We present evidence that the libraries contain overlapping inserts starting at most of the positions within the genome, making these libraries suitable for genomic SELEX...
  47. ncbi Direct targeting and rapid isolation of BAC clones spanning a defined chromosome region
    Edwige Isidore
    Institute of Plant Biology, University of Zurich, Zollikerstrasse 107, 8008 Zurich, Switzerland
    Funct Integr Genomics 5:97-103. 2005
    ..The described low-cost cloning strategy is fast and will greatly facilitate direct targeting of genes and large-scale intra- and inter-species comparative genome analysis...
  48. ncbi Systematic search for the Cra-binding promoters using genomic SELEX system
    Tomohiro Shimada
    Nippon Institute for Biological Science, Division of Molecular Biology, Ome, Tokyo 198 0024, Japan
    Genes Cells 10:907-18. 2005
  49. ncbi Screening and characterization of a novel esterase from a metagenomic library
    Yun Jung Kim
    Department of Molecular Science and Technology, College of Engineering, Ajou University, San 5, Woncheon dong, Yeongtong Gu, Suwon 443 749, Republic of Korea
    Protein Expr Purif 45:315-23. 2006
    ..This study demonstrates that functional screening combined with the sequential uses of restriction enzymes to exclude already known enzymes is a useful approach for isolating novel enzymes from a metagenome...
  50. pmc Selection of genomic sequences that bind tightly to Ff gene 5 protein: primer-free genomic SELEX
    Jin Der Wen
    Department of Molecular and Cell Biology, Mail Stop FO 3 1, The University of Texas at Dallas, PO Box 830688, Richardson, TX 75083 0688, USA
    Nucleic Acids Res 32:e182. 2004
    ..Primer-annealing sequences are removed from the genomic library before selection with the target protein and are then regenerated to allow amplification of the selected ..
  51. ncbi Preliminary linkage map of the turkey (Meleagris gallopavo) based on microsatellite markers
    D W Burt
    Department of Genomics and Bioinformatics, Roslin Institute Edinburgh, Roslin, Midlothian, UK
    Anim Genet 34:399-409. 2003
    ..turkey genetic linkage map we decided to map new microsatellite sequences obtained from a GT-enriched turkey genomic library. In different chicken populations more than 35-55% of microsatellites are polymorphic...
  52. ncbi PCR-based approach to SINE isolation: simple and complex SINEs
    Olga R Borodulina
    Laboratory of Eukaryotic Genome Evolution, Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow 119991, Russia
    Gene 349:197-205. 2005
    ..Interestingly, three SINE families of this group are alanine tRNA-derived. Most probably, this tRNA gave rise to short and simple but successful SINEs several times during mammalian evolution...
  53. ncbi Tall fescue genomic SSR markers: development and transferability across multiple grass species
    Malay C Saha
    Forage Improvement Division, The Samuel Roberts Noble Foundation, Inc, 2510 Sam Noble Parkway, Ardmore, OK 73401, USA
    Theor Appl Genet 113:1449-58. 2006
    ..and other forage grass species. A tall fescue genomic library enriched in (GA/CT)( n ) repeats was used to develop primer pairs (PPs) flanking SSRs and assess PP ..
  54. ncbi Direct selection of cDNAs using genomic contigs
    M Lovett
    University of Texas Southwestern Medical Center at Dallas, Dallas, Texas
    Curr Protoc Hum Genet . 2001
    ..In the , uncloned cDNA or cDNA from a library with highly repetitive sequences suppressed are hybridized to biot...
  55. pmc A linkage map for the B-genome of Arachis (Fabaceae) and its synteny to the A-genome
    Marcio C Moretzsohn
    EMBRAPA Recursos Geneticos e Biotecnologia, Brasilia, DF, Brazil
    BMC Plant Biol 9:40. 2009
    ..This can be overcome by the utilization of wild species of Arachis, which present the A- and B-genomes in the diploid state, and show high levels of genetic variability...
  56. ncbi Identification of a new gene encoding EPSPs with high glyphosate resistance from the metagenomic library
    Dan Jin
    College of Life Sciences, Key Laboratory of Bio resources and Eco environment, The Ministry of Education, Sichuan University, 610064, Chengdu, China
    Curr Microbiol 55:350-5. 2007
    ..A His-tagged RD fusion protein was produced into E. coli to characterize the enzymatic properties of the RD EPSP protein...
  57. ncbi Gene cloning and biochemical characterization of the BMP-2 of Pinctada fucata
    Tomoyuki Miyashita
    Department of Genetic Engineering, Faculty of Biology Oriented Science and Technology, Kinki University, Kinokawa, Wakayama, Japan
    Biosci Biotechnol Biochem 72:37-47. 2008
    ..The BMP-2 gene was expressed strongly in the inner part of the mantle tissue, corresponding to the nacreous aragonite shell layer. This finding suggests that BMP-2 has a key role in nacreous layer formation...
  58. pmc Sequencing of 6.7 Mb of the melon genome using a BAC pooling strategy
    Víctor M González
    Molecular Genetics Department, Center for Research in Agricultural Genomics CRAG, CSIC IRTA UAB, Jordi Girona 18 26, 08034 Barcelona, Spain
    BMC Plant Biol 10:246. 2010
    ..The development of massively parallel sequencing methods allows envisaging new strategies to obtain long fragments of genomic sequence at higher speed and lower cost than previous Sanger-based methods...
  59. pmc Dynamics of genomic-library enrichment and identification of solvent tolerance genes for Clostridium acetobutylicum
    Jacob R Borden
    Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL 60208, USA
    Appl Environ Microbiol 73:3061-8. 2007
    A Clostridium acetobutylicum ATCC 824 genomic library was constructed using randomly sheared DNA. Library inserts conferring increased tolerance to 1-butanol were isolated using two protocols...
  60. ncbi Isolation and partial characterization of novel genes encoding acidic cellulases from metagenomes of buffalo rumens
    C J Duan
    Guangxi Key Laboratory of Subtropical Bioresources Conservation and Utilization, The Key Laboratory of Ministry of Education for Microbial and Plant Genetic Engineering, Guangxi University, Nanning, Guangxi, People s Republic of China
    J Appl Microbiol 107:245-56. 2009
    ..To clone and characterize genes encoding novel cellulases from metagenomes of buffalo rumens...
  61. ncbi An integrated BAC and genome sequence physical map of Phytophthora sojae
    Xuemin Zhang
    Virginia Bioinformatics Institute, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061 0477, USA
    Mol Plant Microbe Interact 19:1302-10. 2006
    ..sojae and other Phytophthora spp. In one illustration of this value, we have placed the 350 members of a superfamily of putative pathogenicity effector genes onto the map, revealing extensive clustering of these genes...
  62. ncbi Identification of genes conferring arsenic resistance to Escherichia coli from an effluent treatment plant sludge metagenomic library
    Nar Singh Chauhan
    Microbial Genomics and Biotechnology Group, Institute of Genomics and Integrative Biology, Council of Scientific and Industrial Research CSIR, Delhi, India
    FEMS Microbiol Ecol 67:130-9. 2009
    ..ArsN alone resulted in an approximately sixfold higher resistance to sodium arsenate in wild-type Escherichia coli W3110...
  63. ncbi In vitro selection of RNA aptamers derived from a genomic human library against the TAR RNA element of HIV-1
    Marguerite Watrin
    INSERM U869, European Institute of Chemistry and Biology, Pessac, France
    Biochemistry 48:6278-84. 2009
    ..The 5' ACCCAG loop of a1 constitutes a new motif of interaction with the TAR loop...
  64. ncbi A new beta-galactosidase with a low temperature optimum isolated from the Antarctic Arthrobacter sp. 20B: gene cloning, purification and characterization
    Aneta Monika Białkowska
    Institute of Technical Biochemistry ITB, Technical University of Łódź, Stefanowskigo 4 10, 90 924 Łódź, Poland
    Arch Microbiol 191:825-35. 2009
  65. pmc Genome physical mapping from large-insert clones by fingerprint analysis with capillary electrophoresis: a robust physical map of Penicillium chrysogenum
    Zhanyou Xu
    Department of Soil and Crop Sciences, Texas A and M University College Station, TX 77843, USA
    Nucleic Acids Res 33:e50. 2005
  66. pmc The Oryza bacterial artificial chromosome library resource: construction and analysis of 12 deep-coverage large-insert BAC libraries that represent the 10 genome types of the genus Oryza
    Jetty S S Ammiraju
    Arizona Genomics Institute, University of Arizona, Tucson, Arizona 85721 USA
    Genome Res 16:140-7. 2006
  67. pmc An improved method to identify BAC clones using pooled overgos
    Kavitha Madishetty
    Department of Botany and Plant Sciences, University of California, Riverside, CA 92521 0124, USA
    Nucleic Acids Res 35:e5. 2007
    ..The resulting cost savings and reduced human exposure to radiation make the use of highly pooled overgo probes a more attractive approach for screening of BAC libraries from organisms with large genomes...
  68. pmc A genomic-library based discovery of a novel, possibly synthetic, acid-tolerance mechanism in Clostridium acetobutylicum involving non-coding RNAs and ribosomal RNA processing
    Jacob R Borden
    Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL 60208, USA
    Metab Eng 12:268-81. 2010
    We generated a genomic library from sheared Clostridium acetobutylicum ATCC 824 DNA, whereby inserts can be expressed in both directions from the thiolase promoter, P(thl). Serial transfer of library-bearing C...
  69. ncbi Utilization of a zebra finch BAC library to determine the structure of an avian androgen receptor genomic region
    Meizhong Luo
    Department of Plant Sciences, Arizona Genomics Institute, University of Arizona, Tucson, AZ 85721, USA
    Genomics 87:181-90. 2006
    ..Comparison of BAC end sequences and the whole BAC sequence with the chicken genome draft sequence showed a high degree of conserved synteny between the zebra finch and the chicken genome...
  70. ncbi Updating the metagenomics toolbox
    Esther Gabor
    BRAIN Aktiengesellschaft, Zwingenberg, Germany
    Biotechnol J 2:201-6. 2007
    ..However, challenges still lie ahead. Most prominently, the efficient heterologous expression of a plethora of potentially interesting enzymes from unknown source organisms is not readily achieved...
  71. pmc The first generation of a BAC-based physical map of Brassica rapa
    Jeong Hwan Mun
    Brassica Genomics Team, National Institute of Agricultural Biotechnology, Rural Development Administration, 225 Seodun dong, Gwonseon gu, Suwon 441 707, South Korea
    BMC Genomics 9:280. 2008
    ..Integration of a physical map with an existing genetic map by linking genetic markers and BAC clones in the sequencing pipeline provides a crucial resource for the ongoing genome sequencing effort and assembly of whole genome sequences...
  72. ncbi Construction of a subgenomic BAC library specific for chromosomes 1D, 4D and 6D of hexaploid wheat
    Jaroslav Janda
    Laboratory of Molecular Cytogenetics and Cytometry, Institute of Experimental Botany, Sokolovska 6, 77200 Olomouc, Czech Republic
    Theor Appl Genet 109:1337-45. 2004
    ..Their availability should facilitate the analysis of wheat genome structure and evolution, development of cytogenetic maps, construction of local physical maps and map-based cloning of agronomically important genes...
  73. ncbi zA map for sequence analysis of the Arabidopsis thaliana genome
    M Marra
    Washington University Genome Sequencing Center, St Louis, Missouri 63108, USA
    Nat Genet 22:265-70. 1999
  74. pmc Isolation and biochemical characterization of two novel metagenome-derived esterases
    C Elend
    Molekulare Enzymtechnologie, Universitat Duisburg Essen, Lotharstrasse 1, 47057 Duisburg, Germany
    Appl Environ Microbiol 72:3637-45. 2006
    ..The high level of stability of these enzymes together with their unique substrate specificities make them highly useful for biotechnological applications...
  75. pmc Interactions of Escherichia coli RNA with bacteriophage MS2 coat protein: genomic SELEX
    T Shtatland
    Department of Molecular, University of Colorado, Boulder, CO 80309 0347, USA
    Nucleic Acids Res 28:E93. 2000
    ..Such methods may be useful tools for both randomized sequence SELEX and genomic SELEX...
  76. pmc Metagenomic profiling: microarray analysis of an environmental genomic library
    Jonathan L Sebat
    Environmental Research Institute, University of Idaho, Moscow, Idaho 83844 1052, USA
    Appl Environ Microbiol 69:4927-34. 2003
    ..Metagenomic profiling offers an effective approach for rapidly characterizing many clones and identifying the clones corresponding to unidentified species of microorganisms...
  77. pmc From raw materials to validated system: the construction of a genomic library and microarray to interpret systemic perturbations in Northern bobwhite
    Arun Rawat
    Department of Biological Sciences, University of Southern Mississippi, Hattiesburg, MS, USA
    Physiol Genomics 42:219-35. 2010
    ..Furthermore, the systemic impact of dinitrotoluenes on liver function appears conserved across species as PPAR signaling is similarly affected in fathead minnow liver tissue after exposure to 2,4-DNT...
  78. pmc Boudicca, a retrovirus-like long terminal repeat retrotransposon from the genome of the human blood fluke Schistosoma mansoni
    Claudia S Copeland
    Department of Tropical Medicine, School of Public Health and Tropical Medicine, Tulane University Health Sciences Center, New Orleans, Louisiana 70112, USA
    J Virol 77:6153-66. 2003
    ..By reverse transcription-PCR, mRNA transcripts were detected in the sporocyst, cercaria, and adult developmental stages of S. mansoni, indicating that Boudicca is actively transcribed in this trematode...
  79. pmc Structural characterization of SIL, a gene frequently disrupted in T-cell acute lymphoblastic leukemia
    P D Aplan
    Naval Medical Branch, National Cancer Institute, Bethesda, Maryland 20889 5105
    Mol Cell Biol 11:5462-9. 1991
    ..The genomic structure of SIL was also analyzed. The gene consists of 18 exons distributed over 70 kb, with the 5' portion of the gene demonstrating alternate exon utilization...
  80. ncbi Construction and characterization of three BAC libraries for analysis of the chicken genome
    M K Lee
    Department of Soil and Crop Sciences, 2123 TAMUS, Texas A and M University, College Station, Texas 77843 2123, USA
    Anim Genet 34:151-2. 2003
  81. ncbi Biomedical applications and studies of molecular evolution: a proposal for a primate genomic library resource
    Evan E Eichler
    Department of Genetics, Case Western Reserve University School of Medicine and University Hospitals of Cleveland, Cleveland, Ohio 44106, USA
    Genome Res 12:673-8. 2002
    ..Based on existing BAC library resources, we propose the construction of a panel of primate BAC libraries from phylogenetic anchor species for the purpose of comparative medicine as well as studies of genome evolution...
  82. pmc Integration of Cot analysis, DNA cloning, and high-throughput sequencing facilitates genome characterization and gene discovery
    Daniel G Peterson
    Center for Applied Genetic Technologies and Department of Crop and Soil Sciences, University of Georgia, Athens, Georgia 30602, USA
    Genome Res 12:795-807. 2002
    ..High-throughput sequencing of Cot clones may be a means of "capturing" the sequence complexity of eukaryotic genomes at unprecedented efficiency...
  83. pmc Development of microsatellite markers from an enriched genomic library for genetic analysis of melon (Cucumis melo L.)
    Patricia Silva Ritschel
    Cellular Biology Department, IB University of Brasília UnB Campus Universitário, Asa Norte, CEP 70 910 900, Brasilia, DF, Brazil
    BMC Plant Biol 4:9. 2004
    ..Genomic microsatellite enriched libraries can be an efficient alternative for marker development in such species...
  84. pmc New softwares for automated microsatellite marker development
    Wellington Martins
    Department of Computer Science, Catholic University of Goias, Brazil
    Nucleic Acids Res 34:e31. 2006
    ..The software is freely available and simple to install in both Windows and Unix-based operating systems. Here we demonstrate the software by developing primer pairs for 427 new candidate markers for peanut...
  85. ncbi Lateral gene transfer and phylogenetic assignment of environmental fosmid clones
    Camilla L Nesbø
    Department of Biochemistry and Molecular Biology, Dalhousie University and Genome Atlantic, 5850 College Street, Halifax, Nova Scotia, Canada, B3H1X5
    Environ Microbiol 7:2011-26. 2005
    ..In several cases, we can infer co-transfer of functionally related genes, and generate hypotheses about mechanism and ecological significance of transfer...
  86. pmc A highly redundant BAC library of Atlantic salmon (Salmo salar): an important tool for salmon projects
    Jim Thorsen
    Department of Basic Sciences and Aquatic Medicine, Section of Genetics, Norwegian School of Veterinary Science, P O Box 8146 Dep, N 0033 Oslo, Norway
    BMC Genomics 6:50. 2005
    ..Furthermore, large-scale mapping can utilize highly redundant libraries in genome projects, and hence provide valuable data on the genome structure...
  87. ncbi Development of a genomic library of near isogenic lines (NILs) in melon (Cucumis melo L.) from the exotic accession PI161375
    Iban Eduardo
    Departament de Genètica Vegetal, Laboratori de Genètica Molecular Vegetal CSIC IRTA, Carretera de Cabrils s n, 08348 Cabrils, Barcelona, Spain
    Theor Appl Genet 112:139-48. 2005
    ..The NILs can also be used as pre-competitive breeding lines in melon breeding projects...
  88. pmc E-RNAi: a web application to design optimized RNAi constructs
    Zeynep Arziman
    Boveri Group Signaling and Functional Genomics, German Cancer Research Center, Im Neuenheimer Feld 580, D 69120 Heidelberg, Germany
    Nucleic Acids Res 33:W582-8. 2005
    ..We here describe a web-based tool to evaluate and design optimized dsRNA constructs. Moreover, the application also gives access to published predesigned dsRNAs. The E-RNAi web application is available at
  89. pmc Environmental genomics of "Haloquadratum walsbyi" in a saltern crystallizer indicates a large pool of accessory genes in an otherwise coherent species
    Boris A Legault
    Evolutionary Genomics Group, Division de Microbiologia, Universidad Miguel Hernandez, Apartado 18, San Juan 03550, Alicante, Spain
    BMC Genomics 7:171. 2006
    ..Similar studies carried out in other extreme environments have revealed very little diversity in gene content among the cell lineages present...
  90. ncbi Isolation of a low-temperature adapted lipolytic enzyme from uncultivated micro-organism
    C Roh
    School of Chemical and Biological Engineering, Institute of Molecular Biology and Genetics, Seoul National University, Seoul, South Korea
    J Appl Microbiol 105:116-23. 2008
    ..The aim of the study was to isolate a novel lipolytic enzyme from the activated sludge of uncultured micro-organisms...
  91. pmc Utility of different gene enrichment approaches toward identifying and sequencing the maize gene space
    Nathan Michael Springer
    Center for Plant and Microbial Genomics, Department of Plant Biology, University of Minnesota, St Paul, Minnesota 55108, USA
    Plant Physiol 136:3023-33. 2004
    ..Analysis of the overlap of coverage and bias due to position within a gene suggest that RescueMu, methyl-filtration, and high-Cot methods are at least partially nonredundant...
  92. pmc Rice genome organization: the centromere and genome interactions
    Nori Kurata
    Plant Genetics Laboratory, Genetic Strains Research Center, National Institute of Genetics, 1111 Yata, Mishima, Shizuoka 411 8540, Japan
    Ann Bot 90:427-35. 2002
    ..A genome-wide survey of interactive genetic loci has identified various reproductive barriers that may drive speciation of the rice genome. The significance of these findings in genome organization and evolution is discussed...
  93. ncbi Toward closing rice telomere gaps: mapping and sequence characterization of rice subtelomere regions
    Tae Jin Yang
    Brassica Genomics Team, National Institute of Agricultural Biotechnology, RDA, Suwon, 441 707, Korea
    Theor Appl Genet 111:467-78. 2005
  94. pmc A census of rRNA genes and linked genomic sequences within a soil metagenomic library
    Mark R Liles
    Department of Plant Pathology Gaylord Nelson Institute of Environmental Studies, University of Wisconsin Madison, 1630 Linden Drive, Madison, WI 53706, USA
    Appl Environ Microbiol 69:2684-91. 2003
    ..This study is the first step in using genomics to reveal the physiology of as-yet-uncultured members of the Acidobacterium division...
  95. pmc Rainbow smelt (Osmerus mordax) genomic library and EST resources
    K R von Schalburg
    Centre for Biomedical Research, University of Victoria, Victoria, BC V8W 3N5, Canada
    Mar Biotechnol (NY) 10:487-91. 2008
    ..They also facilitate identification of genes important to fish and direct us toward new technologies for other studies in fish biology...
  96. ncbi High-Cot sequence analysis of the maize genome
    Yinan Yuan
    Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907 1392, USA
    Plant J 34:249-55. 2003
  97. pmc Characterization of a forest soil metagenome clone that confers indirubin and indigo production on Escherichia coli
    He Kyoung Lim
    Division of Applied Biology, College of Natural Resources and Life Science, Dong A University, Busan 604 714, Republic of Korea
    Appl Environ Microbiol 71:7768-77. 2005
    ..This finding suggests that these forest soil microbial genes are responsible for producing the pigment with antibacterial activity...
  98. ncbi Broad host range vectors for stable genomic library construction
    Michael D Lynch
    Department of Chemical and Biological Engineering, UCB424 ECCH120, University of Colorado, Boulder, 80309, USA
    Biotechnol Bioeng 94:151-8. 2006
    We describe the construction of 36 stable vectors for genomic library construction in gram-negative species. These vectors contain the pBBR1 replicon that has been shown to stably replicate in every gram-negative species tested...
  99. ncbi Identification of immunogenic polypeptides from a Mycoplasma hyopneumoniae genome library by phage display
    Jonas Kügler
    Abteilung Biotechnologie, Institut für Biochemie und Biotechnologie, Technische Universitat Braunschweig, Braunschweig, Germany
    Appl Microbiol Biotechnol 80:447-58. 2008
    ..The fragmented genome of M. hyopneumoniae was cloned into a phage display vector, and the genomic library was packaged using the helperphage Hyperphage to enrich open reading frames (ORFs)...
  100. pmc FSY1, a novel gene encoding a specific fructose/H(+) symporter in the type strain of Saccharomyces carlsbergensis
    P Goncalves
    Centro de Recursos Microbiológicos, Biotechnology Unit, Faculty of Sciences and Technology, New University of Lisbon, 2825 114 Caparica, Portugal
    J Bacteriol 182:5628-30. 2000
    ..Fsy1p is only distantly related to the Hxt proteins that mediate facilitated diffusion of glucose and fructose in Saccharomyces cerevisiae and related species...
  101. pmc A BAC-based physical map of the Drosophila buzzatii genome
    Josefa González
    Departament de Genetica i de Microbiologia, Universitat Autonoma de Barcelona, 08193 Bellaterra Barcelona, Spain
    Genome Res 15:885-92. 2005
    ..We constructed a Bacterial Artificial Chromosome (BAC) genomic library of D. buzzatii using the shuttle vector pTARBAC2.1...

Research Grants62

  1. Validating metabolic pathways in the intracellular pathogen Chlamydia trachomatis
    DEREK JAMES FISHER; Fiscal Year: 2010
    ..This research application seeks to identify new ways to treat and prevent chlamydial infections, as well as to elucidate unknown features of the chlamydial metabolism. ..
  2. Ehrlichia chaffeensis protective proteins
    David H Walker; Fiscal Year: 2010
    ..Both T-cell screening of a genomic library and proteomic approaches will be utilized to identify E...
    Sergei M Mirkin; Fiscal Year: 2012
    ..repeat expansions or a decreased rate of repeat contractions using gene disruption with a mutagenized yeast genomic library. In mammalian cells, we will evaluate replication of various expandable repeats in the pSV2neo episome using ..
  4. Signaling Networks in Chemotaxis and Cytokinesis
    Peter N Devreotes; Fiscal Year: 2013
    ..We also report on a genomic library complementation approach to identify chemically-induced mutants...
  5. Identification of Francisella tularensis extracellular proteins expressed in vivo
    JAMES EDWARD BINA; Fiscal Year: 2010
    ..In accordance with our research goals, three scientific aims are proposed. In specific aim 1 a genomic library will be constructed...
    Anath Das; Fiscal Year: 1991
    ..The genomic clones corresponding to the cDNA clones will be isolated by screening a genomic library using cDNA clones as probe...
  7. Evaluation of Novel Antimicrobial Agents and a Novel Shuttle Vector
    Mark R Liles; Fiscal Year: 2010 evaluation of a novel Gram- negative shuttle vector, and the next generation of microbial community genomic library for future development of this technology...
    Andrew Leiter; Fiscal Year: 1990
    ..To accomplish these objectives the rat PP gene will be isolated and characterized from a genomic library. A PYY cDNA, which I have recently isolated from a bacteriophage lambda gt11 cDNA expression library prepared ..
    David Mount; Fiscal Year: 1999
    ..The mouse BSC gene will be cloned from a genomic library. Its intron-exon structure will be determined, and the 5'-promoter region will be cloned and sequenced...
    MARK MAIDEN; Fiscal Year: 2003
    ..The experimental design will be to first isolate clones of the two genes by screening a B. forsythus genomic library (Specific Aims 1 and 3). The cloned genes will be sequences and expressed in E. coli...
    Amybeth Cohen; Fiscal Year: 2002
    ..We have produced antisera against these proteins, and have isolated two of the genomic clones: a screen of a genomic library should allow isolation of the third clone...
    ANNE DRANGINIS; Fiscal Year: 1999
    ..A FLO11 mutant Saccharomyces strain will be transformed with a plasmid genomic library from C. albicans. Transformants will be selected and assayed for invasiveness...
    Randy Schekman; Fiscal Year: 1990
    ..antiserum was used to identify a clone of the yeast heavy chain gene (CHC1) expressed in a Lambdagtll genomic library. The identity of this insert was confirmed by hybrid-selected translation of heavy chain mRNA followed by ..
    CARLOS HIRSCHBERG; Fiscal Year: 2000
    ..We will transform, with a genomic library made from wild-type DNA, a mutant of K. lacis...
    Philip Youderian; Fiscal Year: 2004
    ..More important, these P22+insert particles are powerful genetic elements that allow us to order a genomic library of M. xanthus inserts by the functional approach of complementation of Salmonella auxotrophies...
    LINVILLE MEADOWS; Fiscal Year: 1990
    ..We will use the oligonucleotides as probes to screen a genomic library of human DNA (or a cDNA library prepared from Daudi cells) to isolate the gene coding for the receptor...
    ROBERT DESNICK; Fiscal Year: 1991
    ..The oligonucleotide mixture has also been used to select clones from an X-chromosome-specific genomic library constructed in phage lambda...
    John Reveille; Fiscal Year: 1992
    ..2kb RFLP. The new probe thus generated will be utilized to screen a cosmid genomic library from an AS patient with the 9...
    Marvin Paule; Fiscal Year: 1992
    ..A genomic library, which because of the small genomic complexity of Acanthamoeba consists of only 9200 plaques, will be ..
    LOWELL HOKIN; Fiscal Year: 1991
    ..This system will also be used to test for involvement of signal recognition particle. A brine shrimp genomic library will be constructed in order to examine the genomic structure of the genes encoding the subunits...
    Pranela Rameshwar; Fiscal Year: 2000 required and this will be obtained after cloning of the human NK-1 gene from a commercially available genomic library. Different upstream sequences of the NK-1 gene will be used in aim 3 to prepare heterologous reporter ..
    Abram Gabriel; Fiscal Year: 1991
    ..2) characterize the protein coding alpha and beta tubulin genes and their expression, through cDNA and genomic library construction, DNA and RNA filter hybridization, and specific transcription rare measurements...
    Ben Shen; Fiscal Year: 2001
    ..2 kilobase DNA fragment and map the entire blm cluster by hybridization of a genomic library of S. verticillus with the cosmid clone containing the above DNA...
  24. An expression system for protein production in eggs
    Lei Zhu; Fiscal Year: 2002
    ..Regulatory sequences from the Ovalbumin (Ov) gene will be isolated from a chicken genomic library. Expression vectors with Ov regulatory sequences will be constructed and tested in primary tubular gland ..
    Jan Hu; Fiscal Year: 2001
    ..The porcine amelogenin gene(s) is isolated from a male porcine genomic library. The structure of the gene is determined by the characterization of PCR amplification products, restriction ..
    RADHESHYAM JAYASWAL; Fiscal Year: 1993
    ..mutated gene: This will be achieved by cloning the 5' flanking sequences of the mutated gene and probing a genomic library of S. aureus followed by deletion, complementation, nucleotide sequencing, and Northern analysis...
    Gerold Bepler; Fiscal Year: 1999
    ..Contig gaps will be filled with bacterial artificial chromosome (BAC) clones by screening the BAC human genomic library available from GenomeSystems, Inc...
    Mark Grossman; Fiscal Year: 1991
    ..They will then be used to isolate clones from screening a human genomic library. The genomic clones will be characterized by restriction mapping, nucleotide sequencing, and S-1 mapping to ..
    Ellen Kraig; Fiscal Year: 2002
    ..Clone genes that encode other T cell epitopes by direct screening of an Aa genomic library. The identities of the Aa proteins recognized by the T cell hybridomas will be determined and recombinant ..
    Ann Progulske Fox; Fiscal Year: 1990
    ..gingivalis chromosomal DNA via an expression vector (plasmid) into Escherichia coli in order to produce a genomic library. Those E. coli transformants of the library which encode B...
    Laura Richman; Fiscal Year: 2002
    ..Hayward s laboratory, creation of a viral genomic library to obtain additional clones to help elucidate the subfamily of the virus, possible utilization of ..
    JOHN SPITZNAGEL; Fiscal Year: 2001
    ..A genomic library, constructed in a broad range mobilization cosmid vector, will be used in complementation studies with a non-..
    Mitchell Abrahamsen; Fiscal Year: 2002
    ..The following specific aims are proposed: 1) Generate and "end sequence" a random small-insert C. parvum genomic library; 2) Generate and "end sequence" large-insert (15-20 kb) C...
    Leena Pulkkinen; Fiscal Year: 2002
    ..the following specific aims are proposed: 1) Isolation and characterization of LAMC2 lambda-clones from mouse genomic library; 2) Construction of the targeting vector based on the data obtained from 1); 3) Generation of three allelic ..
    Immo Scheffler; Fiscal Year: 1991
    ..Cloned, human probes are available from the screening of a genomic library of one of these hybrids, and we also have several cloned human satellite probes from other laboratories...
    Thomas Gill; Fiscal Year: 1992
    ..A genomic library will be constructed from the R21 strain, and the class I loci will be mapped using overlapping cosmids...
    Kenneth Weiss; Fiscal Year: 1993
    ..This is responsive to NIDR's Small Grants Program, as a research retraining initiative for Kenneth M. Weiss, and an extension of ongoing methods to a new area for Frank H. Ruddle...
  38. Selenoprotein Targeting in Prostate Carcinogenesis
    David Menter; Fiscal Year: 2004
    ..Aim 1: Will isolate and characterize the SBP2 gene. An SBP2 cDNA clone will be used to screen a mouse 129S genomic library. Sequencing will be performed to determine the intron-exon boundaries and the most useful exon for targeting...
  39. Harvard-Partners Genome Center
    Raju Kucherlapati; Fiscal Year: 2002
    ..We will use bacterial artificial chromosome (BAC) clones from a genomic library. The genomic library was prepared at RPCI using DNA from C57B1/6J mouse strain...
    LARRY BARNES; Fiscal Year: 1990
    ..The gene (AFP) for Ap4A phosphorylase will be isolated from a yeast genomic library using an immunologic or oligonucleotide probe...
    Emily Kim; Fiscal Year: 1999
    ..To isolate a sweetness receptor through expression cloning, a human genomic library and a cDNA expression library from taste receptor cells will be screened using a variety of affinity ..
    Felix Friedberg; Fiscal Year: 1991 isolate and characterize each of the human calmodulin genes using both chromosome-specific libraries and a genomic library. This would allow analysis of the two or more active genes thus clarifying the distinguishing and common ..
    James Grant; Fiscal Year: 1991
    ..The gene for the 20,000 dalton regulatory light chain of smooth muscle will be isolated by screening a rat genomic library using the full length regulatory light chain cDNA already isolated by the applicant...
    JOSEPH SORGE; Fiscal Year: 1990
    ..2) The second step is to construct a chromosome specific human genomic library in this vector...
    J Sutcliffe; Fiscal Year: 1993
    ..1. We will assemble a nearly complete mouse chromosome 16-specific genomic library that can be rapidly and repeatedly analyzed by hybridization. 2...
  46. Genetic Regulation of Mushroom Development
    John Horton; Fiscal Year: 2001
    ..A delta-frtl null strain will be transformed by DNA from a "non-self" genomic library, looking for complementation...
    Michael Hall; Fiscal Year: 2000
    ..The complete gene sequence will also be obtained from a human genomic library. The normal and mutant cDNAs will be introduced into non-phagocytic eukaryotic cell lines, and the ability of ..
    J Gargus; Fiscal Year: 1993
    ..A cosmid genomic library has been formed from the mutant DNA and the mutant channel gene has been localized to a specific volume in ..
    Najib El Sayed; Fiscal Year: 1999
    ..Telomere-proximal sequences containing the telomere-linked vsgs will be identified in clones of the sheared genomic library. The purpose of this first phase is to enhance early gene discovery and to provide markers that will be ..
  50. Characterization of the Murine pcy Mutation
    David Woo; Fiscal Year: 2005 the molecular level, we will (1) isolate the minimal pcy interval in BACs contigs using the RPCI-23 mouse genomic library for sequencing by the NIH mouse genome sequencing project and (2) identify the pcy gene by systematically ..
    Paul Leavis; Fiscal Year: 2000
    ..we will use a partial protein sequence to prepare degenerate oligonucleotides to identify the gene in a human genomic library. The clone will be partially sequenced, and a specific probe will be used to screen a cDNA library...
    JEANNE DIETZ BAND; Fiscal Year: 1993
    ..Phase I will use P1 and cosmid genomic library screening strategies to isolate clones representative of c-myc, N-myc, and L-myc oncogene sequences...
    Basil Rigas; Fiscal Year: 1993
    ..The investigators' approach is as follows: the DNA of several equivalents of a genomic library is isolated, and using an end fragment of clone A that represents a starting point for the mapping, the ..
  54. A screen for damage response genes using recombination
    Alexander Bishop; Fiscal Year: 2006
    ..From this work I hope to identify the genes that will form the basis of my future work. ..
  55. Molecular Haplotyping of Large Genomic Distances of DNA
    JOHN LANGMORE; Fiscal Year: 2002
    ..The specific aims are to 1) create a genomic library of high molecular weight DNA molecules, 2) devise a technique to join the ends of the high molecular weight ..
    Fiona Levy; Fiscal Year: 1999
    ..the structural organization and regulatory regions of the murine subunit e gene by screening a mouse genomic library and characterizing the isolated clones using DNA sequencing analysis, restriction digest mapping, Southern ..
  57. Genome-wide discovery of beta cell gene control elements
    Claudia Kappen; Fiscal Year: 2004
    ..From a human genomic library, DNA fragments carrying beta cell-specific enhancers will be selected by virtue of their ability to drive ..
  58. High Fidelity Genomic Cloning And Amplification
    RONALD GODISKA; Fiscal Year: 2007
    ..Improved sequence information will accelerate the advances in biotechnology and increase the potential of individual and comparative genomic analyses. [unreadable] [unreadable] [unreadable]..
  59. The Psp response of Yersinia enterocolitica
    Andrew Darwin; Fiscal Year: 2008
    ..Therefore, by understanding the Psp system we will gain further insight into the essential ability of bacteria to respond to stressful conditions that occur during host infection. ..
    Abram Gabriel; Fiscal Year: 2004
    ..Further we will determine whether the properties of this novel mutant are generalizable to the ubiquitous family of retrotransposons whose RTs have not previously been studied. ..
    Ronald Parry; Fiscal Year: 2001
    ..The potential use of the CmaA gene for construction of hybrid peptide antibiotics containing L-alloisoleucine or coronamic acid will also be explored, using surfactin biosynthesis in B. subtilis as a model system. ..
  62. In Vitro Reprogramming of Somatic Cells into Pluripotent ES-like Cells
    Rudolf Jaenisch; Fiscal Year: 2008