platelet adhesiveness

Summary

Summary: The process whereby PLATELETS adhere to something other than platelets, e.g., COLLAGEN; BASEMENT MEMBRANE; MICROFIBRILS; or other "foreign" surfaces.

Top Publications

  1. ncbi Platelet-collagen interaction: is GPVI the central receptor?
    Bernhard Nieswandt
    Department of Vascular Biology, Rudolf Virchow Center for Experimental Biomedicine Versbacher, Wurzburg, Germany
    Blood 102:449-61. 2003
  2. pmc Neutrophil granulocyte-dependent proteolysis enhances platelet adhesion to the arterial wall under high-shear flow
    N Wohner
    Department of Medical Biochemistry, Semmelweis University, Budapest, Hungary
    J Thromb Haemost 8:1624-31. 2010
  3. ncbi Fibrinogen deposition at the postischemic vessel wall promotes platelet adhesion during ischemia-reperfusion in vivo
    S Massberg
    Ludwig Maximilians University, Institute for Surgical Research, Klinikum Grosshadern, Munich, Germany
    Blood 94:3829-38. 1999
  4. pmc Activation-independent platelet adhesion and aggregation under elevated shear stress
    Zaverio M Ruggeri
    The Scripps Research Institute MEM 175, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Blood 108:1903-10. 2006
  5. pmc Signaling during platelet adhesion and activation
    Zhenyu Li
    Department of Medicine, University of Kentucky, Lexington, USA
    Arterioscler Thromb Vasc Biol 30:2341-9. 2010
  6. pmc Impaired platelet responses to thrombin and collagen in AKT-1-deficient mice
    Juhua Chen
    Joseph J Jacobs Center for Thrombosis and Vascular Biology, Department of Molecular Cardiology, Joseph J Jacobs Center for Thrombosis and Vascular Biology, Cleveland Clinic Foundation, NB50, 9500 Euclid Ave, Cleveland, OH 44195, USA
    Blood 104:1703-10. 2004
  7. ncbi Platelets: physiology and biochemistry
    Kerstin Jurk
    Department of Anaesthesiology and Intensive Care, Experimental and Clinical Haemostasis, University Hospital Munster, Munster, Germany
    Semin Thromb Hemost 31:381-92. 2005
  8. ncbi The effect of topography of polymer surfaces on platelet adhesion
    Li Buay Koh
    School of Materials Science and Engineering, Nanyang Technological University, Singapore 639798, Singapore
    Biomaterials 31:1533-45. 2010
  9. ncbi In vitro hemocompatibility of self-assembled monolayers displaying various functional groups
    Claudia Sperling
    Department of Biocompatible Materials, Leibniz Institute of Polymer Research Dresden, The Max Bergmann Center of Biomaterials Dresden, 01069 Dresden, Germany
    Biomaterials 26:6547-57. 2005
  10. ncbi Role and regulation of phosphatidylinositol 3-kinase β in platelet integrin α2β1 signaling
    Alessandra Consonni
    Department of Biochemistry, University of Pavia, Italy
    Blood 119:847-56. 2012

Research Grants

  1. Initiation and Regulation of Platelet Thrombus Formation
    ZAVERIO RUGGERI; Fiscal Year: 2004
  2. Platelet interactions with vessel wall components
    ZAVERIO RUGGERI; Fiscal Year: 2005
  3. Mechanisms of Thrombus Instability
    ZAVERIO RUGGERI; Fiscal Year: 2007
  4. Structure and function of platelet membrane glycoproteins
    ZAVERIO RUGGERI; Fiscal Year: 2008
  5. Role of von Willebrand Factor in Platelet Thrombus Formation
    Zaverio M Ruggeri; Fiscal Year: 2010
  6. Role of JAM Family in Angiogenesis
    Ulhas P Naik; Fiscal Year: 2010
  7. Modulation of integrin function by cytoplasmic signals
    Ulhas Naik; Fiscal Year: 2007
  8. INTERACTION OF OSTEOPONTIN WITH ADHESION RECEPTORS
    JOEL BENNETT; Fiscal Year: 2002
  9. Blood Systems Biology
    Lawrence Brass; Fiscal Year: 2008
  10. CHARACTERIZATION OF A NOVEL G PROTEIN IN PLATELETS
    Lawrence Brass; Fiscal Year: 2005

Detail Information

Publications231 found, 100 shown here

  1. ncbi Platelet-collagen interaction: is GPVI the central receptor?
    Bernhard Nieswandt
    Department of Vascular Biology, Rudolf Virchow Center for Experimental Biomedicine Versbacher, Wurzburg, Germany
    Blood 102:449-61. 2003
    ..This review discusses these recent developments and proposes possible mechanisms for how GPVI acts in concert with other receptors and signaling pathways to initiate hemostasis and arterial thrombosis...
  2. pmc Neutrophil granulocyte-dependent proteolysis enhances platelet adhesion to the arterial wall under high-shear flow
    N Wohner
    Department of Medical Biochemistry, Semmelweis University, Budapest, Hungary
    J Thromb Haemost 8:1624-31. 2010
    ..Under high shear stress platelets adhere preferentially to the adventitia layer of the arterial vessel wall in a von Willebrand factor (VWF)-dependent manner...
  3. ncbi Fibrinogen deposition at the postischemic vessel wall promotes platelet adhesion during ischemia-reperfusion in vivo
    S Massberg
    Ludwig Maximilians University, Institute for Surgical Research, Klinikum Grosshadern, Munich, Germany
    Blood 94:3829-38. 1999
    ..Since platelets are demonstrated here to induce tyrosine phosphorylation in endothelial cells, platelet recruitment might contribute to the development of an inflammatory reaction during I/R...
  4. pmc Activation-independent platelet adhesion and aggregation under elevated shear stress
    Zaverio M Ruggeri
    The Scripps Research Institute MEM 175, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Blood 108:1903-10. 2006
    ..Platelet and VWF modulation by hydrodynamic force is a mechanism for activation-independent aggregation that may support thrombotic arterial occlusion...
  5. pmc Signaling during platelet adhesion and activation
    Zhenyu Li
    Department of Medicine, University of Kentucky, Lexington, USA
    Arterioscler Thromb Vasc Biol 30:2341-9. 2010
    ..Recent studies have provided novel insight into the molecular mechanisms of these processes...
  6. pmc Impaired platelet responses to thrombin and collagen in AKT-1-deficient mice
    Juhua Chen
    Joseph J Jacobs Center for Thrombosis and Vascular Biology, Department of Molecular Cardiology, Joseph J Jacobs Center for Thrombosis and Vascular Biology, Cleveland Clinic Foundation, NB50, 9500 Euclid Ave, Cleveland, OH 44195, USA
    Blood 104:1703-10. 2004
    ..As a consequence of impaired alpha(IIb)beta(3) activation and platelet aggregation, Akt-1 null mice showed significantly longer bleeding times than wild-type mice...
  7. ncbi Platelets: physiology and biochemistry
    Kerstin Jurk
    Department of Anaesthesiology and Intensive Care, Experimental and Clinical Haemostasis, University Hospital Munster, Munster, Germany
    Semin Thromb Hemost 31:381-92. 2005
    ..Finally, special emphasis is placed on important stimulatory and inhibitory signaling pathways that modulate platelet function...
  8. ncbi The effect of topography of polymer surfaces on platelet adhesion
    Li Buay Koh
    School of Materials Science and Engineering, Nanyang Technological University, Singapore 639798, Singapore
    Biomaterials 31:1533-45. 2010
    ..The results signify that topographical manipulation of thrombogenic surfaces of biodegradable polymers is a feasible approach for reducing their thrombogenicity...
  9. ncbi In vitro hemocompatibility of self-assembled monolayers displaying various functional groups
    Claudia Sperling
    Department of Biocompatible Materials, Leibniz Institute of Polymer Research Dresden, The Max Bergmann Center of Biomaterials Dresden, 01069 Dresden, Germany
    Biomaterials 26:6547-57. 2005
    ..However, the coagulation and platelet activation did not simply correlate with any surface property and were therefore concluded to be determined by a superposition of contact activation and platelet adhesion...
  10. ncbi Role and regulation of phosphatidylinositol 3-kinase β in platelet integrin α2β1 signaling
    Alessandra Consonni
    Department of Biochemistry, University of Pavia, Italy
    Blood 119:847-56. 2012
    ..These results demonstrate that Pyk2 regulates PI3Kβ downstream of integrin α2β1, and document a novel role for Pyk2 and PI3Kβ in integrin α2β1 promoted inside-out activation of integrin αIIbβ3 and thrombus formation...
  11. pmc The catastrophe revisited: blood compatibility in the 21st Century
    Buddy D Ratner
    University of Washington Engineered Biomaterials UWEB, University of Washington, Seattle, WA 98195, USA
    Biomaterials 28:5144-7. 2007
    ..Hypothesis 5-We now have better blood compatible materials but the regulatory and economic climate prevent adoption in clinical practice...
  12. ncbi Small calibre polyhedral oligomeric silsesquioxane nanocomposite cardiovascular grafts: influence of porosity on the structure, haemocompatibility and mechanical properties
    Maqsood Ahmed
    Centre for Nanotechnology and Regenerative Medicine, University College London, London, UK
    Acta Biomater 7:3857-67. 2011
    ..Whilst the different graft samples induced varying levels of cytokine secretion in vitro, no distinct pattern suggesting a non-trivial relationship was observed...
  13. ncbi Inherited bleeding disorders: disorders of platelet adhesion and aggregation
    Indra Ramasamy
    Department of Chemical Pathology, Newham General Hospital, Glen Road, London E13 8RU, UK
    Crit Rev Oncol Hematol 49:1-35. 2004
    ..As progress advances towards individualisation of therapy the phenotypic bleeding tendency of each patient becomes relevant...
  14. pmc Platelet adhesion from shear blood flow is controlled by near-wall rebounding collisions with erythrocytes
    A A Tokarev
    National Research Center for Hematology, Russian Academy of Medical Sciences, Moscow, Russia
    Biophys J 100:799-808. 2011
    ..This finding reveals the mechanism by which erythrocytes physically control platelet hemostasis...
  15. pmc Platelet adhesion and degranulation induce pro-survival and pro-angiogenic signalling in ovarian cancer cells
    Karl Egan
    Molecular and Cellular Therapeutics, The Royal College of Surgeons in Ireland, Dublin, Ireland
    PLoS ONE 6:e26125. 2011
    ..Thus, ovarian cancer cells with different metastatic potential adhere and activate platelets differentially while both platelets and platelet releasate mediate pro-survival and pro-angiogenic signals in ovarian cancer cells...
  16. pmc Antithrombogenic modification of small-diameter microfibrous vascular grafts
    Craig K Hashi
    University of California, San Francisco, CA, USA
    Arterioscler Thromb Vasc Biol 30:1621-7. 2010
    ..To develop small-diameter vascular grafts with a microstructure similar to native matrix fibers and with chemically modified microfibers to prevent thrombosis...
  17. ncbi Mechanisms initiating platelet thrombus formation
    Z M Ruggeri
    Department of Molecular and Experimental Medicine, Scripps Research Institute, La Jolla, CA 92037, USA
    Thromb Haemost 78:611-6. 1997
    ....
  18. ncbi Initiation of platelet adhesion by arrest onto fibrinogen or translocation on von Willebrand factor
    B Savage
    Roon Research Center for Arteriosclerosis and Thrombosis, Department of Molecular and Experimental Medicine, Scripps Research Institute, La Jolla, La Jolla, California 92037, USA
    Cell 84:289-97. 1996
    ..This eventually allows activated alpha IIb beta 3 to arrest platelets onto vWF under conditions not permissive of direct binding to fibrinogen. The coupling of these different functions may be crucial for thrombogenesis...
  19. pmc Antithrombogenic property of bone marrow mesenchymal stem cells in nanofibrous vascular grafts
    Craig K Hashi
    Department of Bioengineering and Center for Tissue Bioengineering, University of California, Berkeley, CA 94720, USA
    Proc Natl Acad Sci U S A 104:11915-20. 2007
    ..These results demonstrate several favorable characteristics of nanofibrous scaffolds, the excellent patency of small-diameter nanofibrous vascular grafts, and the unique antithrombogenic property of MSCs...
  20. ncbi Platelet interactions with titanium: modulation of platelet activity by surface topography
    J Y Park
    Bone Interface Group, Faculty of Dentistry, Institute of Biomaterials and Biomedical Engineering, University of Toronto, Ont, Canada
    Biomaterials 22:2671-82. 2001
    ....
  21. pmc Glycoprotein VI but not alpha2beta1 integrin is essential for platelet interaction with collagen
    B Nieswandt
    Department of Molecular Oncology, General Surgery, Witten Herdecke University, 42117 Wuppertal, Germany
    EMBO J 20:2120-30. 2001
    ..These data show that GPVI plays the central role in platelet-collagen interactions by activating different adhesive receptors, including alpha2beta1 integrin, which strengthens adhesion without being essential...
  22. ncbi Platelets express functional Toll-like receptor-4
    Graciela Andonegui
    Immunology Research Group, Department of Physiology and Biophysics, Faculty of Medicine, University of Calgary, Calgary, AB T2N 4N1, Canada
    Blood 106:2417-23. 2005
    ..Thus, our data show for the first time that platelets do express functional levels of TLR4, which contribute to thrombocytopenia through neutrophil-dependent pulmonary sequestration in response to LPS...
  23. ncbi Use of synthetic peptides to locate novel integrin alpha2beta1-binding motifs in human collagen III
    Nicolas Raynal
    Department of Biochemistry, University of Cambridge, UK
    J Biol Chem 281:3821-31. 2006
    ..We describe two novel high affinity integrin-binding motifs in human collagen III (GROGER and GLOGEN) and a third motif (GLKGEN) that displays intermediate activity. Each motif was verified using shorter synthetic peptides...
  24. ncbi A novel nanocomposite polymer for development of synthetic heart valve leaflets
    Asmeret G Kidane
    Centre for Nanotechnology, Biomaterials and Tissue Engineering, UCL Division of Surgery and Interventional Science, University College London, London NW3 2PF, UK
    Acta Biomater 5:2409-17. 2009
    ..001) (at 37 degrees C) as the thickness increased from 100microm (51.0+/-3.3Nmm(-1)) to 200microm (63+/-1.5Nmm(-1)). The surface of POSS-PCU was significantly less hydrophilic than that of PCU...
  25. pmc Rac1 is essential for platelet lamellipodia formation and aggregate stability under flow
    Owen J T McCarty
    Centre for Cardiovascular Sciences, The Institute of Biomedical Research, School of Biosciences, University of Birmingham, United Kingdom
    J Biol Chem 280:39474-84. 2005
    ....
  26. pmc Identification of coagulation factor XI as a ligand for platelet apolipoprotein E receptor 2 (ApoER2)
    Tara C White-Adams
    Division of Biomedical Engineering, Oregon Health and Science University School of Medicine, 3303 SW Bond Ave, Portland, OR 97239, USA
    Arterioscler Thromb Vasc Biol 29:1602-7. 2009
    ..The aim of this study was to investigate the mechanisms governing interactions between FXI and platelets...
  27. ncbi The adhesion of blood platelets on fibrinogen surface: comparison of two biochemical microplate assays
    Martina Vanícková
    Department of Biochemistry, Institute of Hematology and Blood Transfusion, U Nemocnice 1, 12820 Prague, Czech Republic
    Platelets 17:470-6. 2006
    ..The ACP method is much more suitable for quantitative determination of platelet adhesion especially in the development and evaluation of haemocompatibility of new biomaterials...
  28. ncbi New perspectives on von Willebrand factor functions in hemostasis and thrombosis
    Grazia Loredana Mendolicchio
    Roon Research Laboratory for Arteriosclerosis and Thrombosis, Division of Experimental Thrombosis and Hemastasis, Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA 92037, USA
    Semin Hematol 42:5-14. 2005
    ..Developments in these areas of research will refine our understanding of the role played by VWF in vascular biology and pathology...
  29. ncbi Glycoprotein Ibalpha and von Willebrand factor in primary platelet adhesion and thrombus formation: lessons from mutant mice
    Wolfgang Bergmeier
    Cardeza Foundation and Department of Medicine, Thomas Jefferson University, 1015 Walnut Street, Philadelphia, PA 19007, USA
    Thromb Haemost 99:264-70. 2008
    ..Recent studies in mutant mice have shed new light on the importance of either molecule for the development of arterial and venous thrombosis. In this review, we summarize the most important aspects from these studies...
  30. ncbi Peroxynitrite-mediated modification of fibrinogen affects platelet aggregation and adhesion
    Pawel Nowak
    Department of General Biochemistry, University of Lodz, Lodz, Poland
    Platelets 13:293-9. 2002
    ..The percentage of ADP-induced platelet aggregation decreased as a function of peroxynitrite-mediated modification of fibrinogen molecule...
  31. ncbi The small proteoglycan decorin supports adhesion and activation of human platelets
    Gianni Guidetti
    Department of Biochemistry, University of Pavia, Italy
    Blood 100:1707-14. 2002
    ..Therefore, these results demonstrate that human platelets can bind to immobilized decorin through integrin alpha(2)beta(1), and that this interaction results in the tyrosine phosphorylation of intracellular proteins...
  32. ncbi Rac, a small guanosine triphosphate-binding protein, and p21-activated kinase are activated during platelet spreading on collagen-coated surfaces: roles of integrin alpha(2)beta(1)
    K Suzuki-Inoue
    Department of Clinical and Laboratory Medicine, Yamanashi Medical University, Nakakoma, Yamanashi, Japan
    Blood 98:3708-16. 2001
    ....
  33. ncbi Platelet adhesion and fusion to endothelial cell facilitate the metastasis of tumor cell in hypoxia-reoxygenation condition
    Na Zhang
    Graduate School of Peking Union Medical College, Beijing 100730, People s Republic of China
    Clin Exp Metastasis 28:1-12. 2011
    ..The adhesion or fusion of platelet to endothelial cell mediated by platelet surface adhesion molecules, which could promote the adhesion of tumor cell with endothelial cells and the tumor metastasis...
  34. ncbi Glow discharge plasma treatment of polyethylene tubing with tetraglyme results in ultralow fibrinogen adsorption and greatly reduced platelet adhesion
    Lan Cao
    Department of Chemical Engineering, University of Washington, Seattle, Washington 98195, USA
    J Biomed Mater Res A 79:788-803. 2006
    ..The improved hemocompatibility is attributed to the unique chemical structure of RFGD tetraglyme that makes it highly protein resistant...
  35. ncbi Genetic and pharmacological analyses of Syk function in alphaIIbbeta3 signaling in platelets
    D A Law
    COR Therapeutics, Inc, South San Francisco, CA, USA
    Blood 93:2645-52. 1999
    ....
  36. ncbi VEGF-E enhances endothelialization and inhibits thrombus formation on polymeric surfaces
    Menno L W Knetsch
    Centre for Biomaterials Research, University of Maastricht, Maastricht, The Netherlands
    J Biomed Mater Res A 93:77-85. 2010
    ..Adsorption of VEGF-E, especially on heparin containing surfaces, provides an economical strategy to improve endothelialization of cardiovascular implants without disturbing blood-compatibility...
  37. pmc In vivo performance of a phospholipid-coated bioerodable elastomeric graft for small-diameter vascular applications
    Lorenzo Soletti
    Department of Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania 15219, USA
    J Biomed Mater Res A 96:436-48. 2011
    ..Further evaluation in vivo will be required to demonstrate complete remodeling of the graft into a native-like artery...
  38. ncbi Low thrombogenicity coating of nonwoven PET fiber structures for vascular grafts
    Sashka Dimitrievska
    Industrial Materials Institute, National Research Council Canada, 75 de Mortagne, Boucherville, QC J4B6Y4, Canada
    Macromol Biosci 11:493-502. 2011
    ....
  39. ncbi The effect of surface roughness on activation of the coagulation system and platelet adhesion in rotary blood pumps
    Jörg Linneweber
    Klinik für Kardiovaskuläre Chirurgie, Charite Universitatsmedizin Berlin, Berlin, Germany
    Artif Organs 31:345-51. 2007
    ..By contrast, the surface roughness had no significant influence on plasma prothrombin F1.2 fragment- and TAT concentrations. Antithrombogenicity was significantly reduced in pumps with inferior metal-finishing quality...
  40. ncbi Super hydrophilic thin film nitinol demonstrates reduced platelet adhesion compared with commercially available endograft materials
    Allan W Tulloch
    Department of Surgery, Division of Vascular Surgery, David Geffen School of Medicine at the University of California Los Angeles, Los Angeles, California 90025, USA
    J Surg Res 171:317-22. 2011
    ....
  41. ncbi Diminished thrombus formation and alleviation of myocardial infarction and reperfusion injury through antibody- or small-molecule-mediated inhibition of selectin-dependent platelet functions
    Gertie J Oostingh
    University of Wurzburg, Rudolf Virchow Center, DFG Research Center for Experimental Biomedicine, Wurzburg Germany
    Haematologica 92:502-12. 2007
    ..P-selectin ctin has been implicated in important platelet functions. However, neither its role in thrombus formation and cardiovascular disorders nor its suitability as a therapeutic target structure is entirely clear...
  42. ncbi Formation of viscoelastic protein layers on polymeric surfaces relevant to platelet adhesion
    Norbert Weber
    Department of Chemistry and Chemical Biology, Rutgers University, 610 Taylor Road, Piscataway, New Jersey 08854, USA
    J Biomed Mater Res A 72:420-7. 2005
    ..These results suggest that material-dependent changes in the thickness and viscoelastic properties of adsorbed fibrinogen-GPIIb-IIIa layers are crucial factors in the binding behavior of platelets to biomaterials...
  43. pmc A critical role of platelet adhesion in the initiation of atherosclerotic lesion formation
    Steffen Massberg
    Deutsches Herzzentrum und 1 Medizinische Klinik, Technische Universitat Munchen, Germany
    J Exp Med 196:887-96. 2002
    ..These findings establish the platelet as a major player in initiation of the atherogenetic process...
  44. ncbi Blood coagulation on biomaterials requires the combination of distinct activation processes
    Claudia Sperling
    Max Bergmann Center of Biomaterials Dresden, Leibniz Institute of Polymer Research Dresden, Dresden, Germany
    Biomaterials 30:4447-56. 2009
    ..Successful surface design strategies for hemocompatible materials therefore need to carefully consider the interplay of both processes...
  45. ncbi Glycocalyx-mimetic dextran-modified poly(vinyl amine) surfactant coating reduces platelet adhesion on medical-grade polycarbonate surface
    A Sen Gupta
    Department of Biomedical Engineering, Case Western Reserve University, Wickenden Building, 10900 Euclid Avenue, Cleveland, OH 44106, USA
    Biomaterials 27:3084-95. 2006
    ..The results suggest potential application of carbohydrate-modified surfactant polymers as a glycocalyx-mimetic non-thrombogenic interfacial coating for blood-contacting biomaterials...
  46. ncbi New insights into the haemostatic function of platelets
    Andrew H Wei
    The Australian Centre for Blood Diseases, Monash University, Department of Haematology, Alfred Hospital, Melbourne, Victoria, Australia
    Br J Haematol 147:415-30. 2009
    ....
  47. pmc The alpha(2) integrin subunit-deficient mouse: a multifaceted phenotype including defects of branching morphogenesis and hemostasis
    Jianchun Chen
    Department of Pathology and Immunology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Am J Pathol 161:337-44. 2002
    ..The alpha(2)beta(1) integrin-null mouse thus exhibits diverse, sometimes subtle, phenotypes consistent with the widespread pattern of alpha(2)beta(1) integrin expression...
  48. pmc Persistence of platelet thrombus formation in arterioles of mice lacking both von Willebrand factor and fibrinogen
    H Ni
    The Center for Blood Research, Boston, Massachusetts 02115, USA
    J Clin Invest 106:385-92. 2000
    ..Platelets of these doubly deficient mice specifically accumulated fibronectin in their alpha-granules, suggesting that fibronectin could be the ligand supporting the platelet aggregation...
  49. pmc Ligand density dramatically affects integrin alpha IIb beta 3-mediated platelet signaling and spreading
    Marketa Jirouskova
    Laboratory of Blood and Vascular Biology, Rockefeller University, New York, NY 10021, USA
    Blood 109:5260-9. 2007
    ..We conclude that 2 fundamentally different signaling mechanisms can be initiated by a single integrin receptor interacting with the same ligand when it is immobilized at different densities...
  50. ncbi A single high-affinity binding site for von Willebrand factor in collagen III, identified using synthetic triple-helical peptides
    Ton Lisman
    Department of Biochemistry, Downing Site, Cambridge CB2 1QW, United Kingdom
    Blood 108:3753-6. 2006
    ..23 either strongly interacted with both VWF and platelets or lacked both VWF and platelet binding. Thus, we identified the sequence RGQOGVMGF (O is hydroxyproline) as the minimal VWF-binding sequence in collagen III...
  51. ncbi A highly stable nonbiofouling surface with well-packed grafted zwitterionic polysulfobetaine for plasma protein repulsion
    Yung Chang
    R and D Center for Membrane Technology and Department of Chemical Engineering, Chung Yuan Christian University, Jhong Li, Taoyuan 320, Taiwan
    Langmuir 24:5453-8. 2008
    ..This work further concludes that a surface with good hemocompatibility can be achieved by the well-packed surface-grafted polySBMA brushes...
  52. ncbi Platelet-endothelial cell interactions during ischemia/reperfusion: the role of P-selectin
    S Massberg
    Ludwig Maximilians University, Institute for Surgical Research, Klinikum Grosshadern, Munich, Germany
    Blood 92:507-15. 1998
    ..Platelet recruitment and subsequent activation might play an important role in the pathogenesis of I/R injury...
  53. ncbi Platelet collagen receptors, signaling and antagonism: emerging approaches for the prevention of intravascular thrombosis
    William Rasican Surin
    Cardiovascular Pharmacology Unit, Central Drug Research Institute, Lucknow 226001, India
    Thromb Res 122:786-803. 2008
    ..These findings have revealed that collagen receptor inhibition is an attractive and secure strategy for the new drug development to prevent intravascular thrombosis...
  54. ncbi Platelet-associated LIGHT (TNFSF14) mediates adhesion of platelets to human vascular endothelium
    Sultan Celik
    Innere Medizin III, Universitat Heidelberg, Heidelberg, Germany
    Thromb Haemost 98:798-805. 2007
    ..LIGHT may thus be implicated in the pathogenesis of atherosclerosis and acute coronary syndrome, as evidenced by serum levels...
  55. ncbi Antagonism of selectin function attenuates microvascular platelet deposition and platelet-mediated myocardial injury after transient ischemia
    José A Barrabés
    Department of Cardiologia, Hospital Universitari Vall d Hebron, Barcelona, Spain
    J Am Coll Cardiol 45:293-9. 2005
    ..The goal of this study was to assess whether selectin blockade reduces myocardial platelet deposition and platelet-mediated injury after transient ischemia...
  56. ncbi New concepts in von Willebrand disease
    J Evan Sadler
    Howard Hughes Medical Institute, Department of Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Annu Rev Med 56:173-91. 2005
    ..Emerging data suggest that VWF level is a useful biomarker for the risk of either bleeding or thrombosis and could be incorporated into a comprehensive approach to treat or prevent these adverse events...
  57. pmc Integrin alpha(v)beta(3) on human endothelial cells binds von Willebrand factor strings under fluid shear stress
    Jing Huang
    Department of Medicine, Biochemistry and Molecular Biophysics, and Howard Hughes Medical Institute, Washington University School of Medicine, St Louis, MO 63110, USA
    Blood 113:1589-97. 2009
    ..These results indicate that VWF strings bind specifically to integrin alpha(v)beta(3) on human endothelial cells...
  58. pmc von Willebrand factor-mediated platelet adhesion is critical for deep vein thrombosis in mouse models
    Alexander Brill
    Immune Disease Institute, 3 Blackfan Circle, Boston, MA 02115, USA
    Blood 117:1400-7. 2011
    ..Our results demonstrate a pathogenetic role for VWF-platelet interaction in flow disturbance-induced venous thrombosis...
  59. pmc Impaired microvascular perfusion in sepsis requires activated coagulation and P-selectin-mediated platelet adhesion in capillaries
    Dan Secor
    Critical Illness Research, Victoria Research Laboratories, Lawson Health Research Institute, London, ON, N6C 2V5, Canada
    Intensive Care Med 36:1928-34. 2010
    ..Since inflammatory and coagulation pathways cross-activate, we tested if stoppage of blood flow in septic capillaries is due to oxidant-dependent adhesion of platelets in these microvessels...
  60. ncbi Structural basis for the platelet-collagen interaction: the smallest motif within collagen that recognizes and activates platelet Glycoprotein VI contains two glycine-proline-hydroxyproline triplets
    Peter A Smethurst
    Department of Biochemistry, University of Cambridge, Downing Site, Cambridge CB2 1QW, United Kingdom
    J Biol Chem 282:1296-304. 2007
    ..Furthermore, we propose that the (GPO)4 motif can support simultaneous binding of two glycoprotein VI molecules, in either a parallel or anti-parallel stacking arrangement, which could play an important role in activation of signaling...
  61. ncbi CD40-CD40 ligand mediates the recruitment of leukocytes and platelets in the inflamed murine colon
    Thorsten Vowinkel
    Department of Molecular and Cellular Physiology, Louisiana State University Health Sciences Center, Shreveport, Louisiana 71130, USA
    Gastroenterology 132:955-65. 2007
    ....
  62. ncbi Endothelial von Willebrand factor recruits platelets to atherosclerosis-prone sites in response to hypercholesterolemia
    Gregor Theilmeier
    Center for Molecular and Vascular Biology, University of Leuven, Leuven, Belgium
    Blood 99:4486-93. 2002
    ..05). In conclusion, hypercholesterolemia primes platelets for recruitment via VWF, GPIb alpha, and P-selectin to lesion-prone sites, before lesions are detectable...
  63. ncbi F11-receptor (F11R/JAM) mediates platelet adhesion to endothelial cells: role in inflammatory thrombosis
    Anna Babinska
    Department of Anatomy Cell Biology, State University of New York, Downstate Medical Center, 450 Clarkson Avenue, Brooklyn, NY 11203, USA
    Thromb Haemost 88:843-50. 2002
    ..Agents that block the F11R-mediated adhesion of platelets to EC may be of therapeutic value in controlling thrombosis and preventing heart attacks and stroke...
  64. ncbi Genetic evidence for a predominant role of PI3Kbeta catalytic activity in ITAM- and integrin-mediated signaling in platelets
    Ilaria Canobbio
    Department of Biochemistry, University of Pavia, via Bassi 21, Pavia, Italy
    Blood 114:2193-6. 2009
    ..These results provide genetic evidence for a crucial and selective role of PI3Kbeta in signaling through GPVI and integrin alpha(IIb)beta(3)...
  65. ncbi Signaling pathways of the F11 receptor (F11R; a.k.a. JAM-1, JAM-A) in human platelets: F11R dimerization, phosphorylation and complex formation with the integrin GPIIIa
    Malgorzata B Sobocka
    Department of Anatomy and Cell Biology, SUNY Downstate Medical Center, Brooklyn, New York 11203, USA
    J Recept Signal Transduct Res 24:85-105. 2004
    ..formation and growth of platelet aggregates and plaques in cardiovascular disorders, resulting in enhanced platelet adhesiveness and hyperaggregability, may serve in the generation of novel therapies in the treatment of inflammatory ..
  66. ncbi Platelets contribute to postnatal occlusion of the ductus arteriosus
    Katrin Echtler
    Deutsches Herzzentrum, Klinik für Herz und Kreislauferkrankungen, Technische Universitat, Munich, Germany
    Nat Med 16:75-82. 2010
    ..A retrospective clinical study revealed that thrombocytopenia is an independent predictor for failure of DA closure in preterm human newborns, indicating that platelets are likely to contribute to DA closure in humans...
  67. pmc Integrin alpha2beta1 mediates outside-in regulation of platelet spreading on collagen through activation of Src kinases and PLCgamma2
    Osamu Inoue
    Department of Pharmacology, University of Oxford, Mansfield Road, Oxford, OX1 3QT, UK
    J Cell Biol 160:769-80. 2003
    ....
  68. ncbi Role of the intracellular domains of GPIb in controlling the adhesive properties of the platelet GPIb/V/IX complex
    Christelle Perrault
    INSERM U311, Etablissement Français du Sang, Alsace, Strasbourg, France
    Blood 101:3477-84. 2003
    ....
  69. ncbi Hemocompatibility of materials used in microelectromechanical systems: platelet adhesion and morphology in vitro
    Brian A Weisenberg
    University of Minnesota, The Biomedical Engineering Institute, Minneapolis, Minnesota 55455, USA
    J Biomed Mater Res 60:283-91. 2002
    ..These results may be helpful in guiding the selection of materials for use in the development of blood-contacting microelectromechanical systems...
  70. pmc Inhibition of platelet adherence to brain microvasculature protects against severe Plasmodium berghei malaria
    Guang Sun
    Departments of Microbiology and Immunology, Louisiana State University Health Sciences Center, Shreveport, Louisiana 71130, USA
    Infect Immun 71:6553-61. 2003
    ..CD41 is not an adhesion molecule for parasitized erythrocytes, these findings support the hypothesis that inhibition of platelet adhesion to the brain microvasculature protects against development of malarial pathogenesis...
  71. ncbi Time sequence of blood activation by nanoporous alumina: Studies on platelets and complement system
    Natalia Ferraz
    Department of Physical and Analytical Chemistry, BMC, Uppsala University, Sweden
    Microsc Res Tech 73:1101-9. 2010
    ..The surface topography of the alumina most probably influence protein transition rate, which in turn affects material platelet activation kinetics...
  72. ncbi Contribution of distinct adhesive interactions to platelet aggregation in flowing blood
    Z M Ruggeri
    Roon Research Center for Arteriosclerosis and Thrombosis, The Division of Experimental Hemostasis and Thrombosis, The Department of Molecular and Experimental Medicine, Scripps Research Institute, La Jolla, CA 92037, USA
    Blood 94:172-8. 1999
    ..The specific function of distinct adhesion pathways in response to changing hemodynamic conditions helps to explain hemostatic and thrombotic processes...
  73. ncbi Contribution of platelet-derived factor Va to thrombin generation on immobilized collagen- and fibrinogen-adherent platelets
    J J Briedé
    Department of Biochemistry, Cardiovascular Research Institute Maastricht, Maastricht University, The Netherlands
    Thromb Haemost 85:509-13. 2001
    ..Our findings suggest a unique role for immobilized collagen in maintaining haemostasis...
  74. ncbi Activation of platelets adhered on amorphous hydrogenated carbon (a-C:H) films synthesized by plasma immersion ion implantation-deposition (PIII-D)
    P Yang
    Department of Physics and Materials Science, City University of Hong Kong, 83 Tat Chee Avenue, Hong Kong, Kowloon, China
    Biomaterials 24:2821-9. 2003
    ..The results show that the behavior of the platelets adhered on the a-C:H films is influenced by their structure and chemical bond, and it appears that protein interaction plays a key role in the activation of the adherent platelets...
  75. pmc The role of platelet adhesion receptor GPIbalpha far exceeds that of its main ligand, von Willebrand factor, in arterial thrombosis
    Wolfgang Bergmeier
    CBR Institute for Biomedical Research and Department of Pathology, Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 103:16900-5. 2006
    ..Thus, GPIbalpha contributes to arterial thrombosis by important adhesion mechanisms independent of the binding to VWF...
  76. ncbi Modification of Si(100) surface by the grafting of poly(ethylene glycol) for reduction in protein adsorption and platelet adhesion
    F Zhang
    Department of Chemical Engineering, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260
    J Biomed Mater Res 56:324-32. 2001
    ..The grafted PEGMA polymer layer on the Si(100) surface exhibited fairly good stability during storage in a buffer solution...
  77. ncbi Platelets adhere to and translocate on von Willebrand factor presented by endothelium in stimulated veins
    P Andre
    Center for Blood Research and the Department of Pathology, Harvard Medical School, Boston, MA, USA
    Blood 96:3322-8. 2000
    ..It is proposed that this process may rapidly recruit platelets to sites of injury or inflammation in veins...
  78. pmc The glycoprotein Ib-IX-V complex is a platelet counterreceptor for P-selectin
    G M Romo
    Department of Medicine, Baylor College of Medicine and Veterans Affairs Medical Center, Houston, Texas 77030, USA
    J Exp Med 190:803-14. 1999
    ..Thus, the GP Ib-IX-V complex mediates platelet attachment to both subendothelium and activated endothelium...
  79. ncbi Circulating platelet-neutrophil complexes are important for subsequent neutrophil activation and migration
    Kristin N Kornerup
    Sackler Institute of Pulmonary Pharmacology and Therapeutics, Division of Pharmaceutical Sciences, School of Biomedical and Health Sciences, King s College, London, UK
    J Appl Physiol (1985) 109:758-67. 2010
    ..Targeting this interaction may be a successful strategy for inflammatory conditions where current therapy fails to provide adequate treatment...
  80. pmc Plasma fibronectin promotes thrombus growth and stability in injured arterioles
    Heyu Ni
    Center for Blood Research and Department of Pathology, Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 100:2415-9. 2003
    ....
  81. ncbi Protein C supports platelet binding and activation under flow: role of glycoprotein Ib and apolipoprotein E receptor 2
    T C White
    Department of Biomedical Engineering, Oregon Health and Science University, Portland, OR 97239, USA
    J Thromb Haemost 6:995-1002. 2008
    ..Binding of APC to EPCR both modifies and induces PAR-1 signaling, but it is unknown if protein C interacts with cells in an alternative manner...
  82. pmc Distinct spatio-temporal Ca2+ signaling elicited by integrin alpha2beta1 and glycoprotein VI under flow
    Mario Mazzucato
    Department of Laboratory Medicine, Centro di Riferimento Oncologico CRO National Cancer Institute, Istituti di Ricovero e Cura a Carattere Scientifico IRCCS, Aviano PN, Italy
    Blood 114:2793-801. 2009
    ..Our results delineate a GPVI-independent signaling role of alpha2beta1 in response to collagen stimulation...
  83. ncbi Arterial thrombosis: relevance of a model with two levels of severity assessed by histologic, ultrastructural and functional characterization
    B Hechler
    UMR S949 INSERM, Universite de Strasbourg, Etablissement Francais du Sang Alsace, Strasbourg, France
    J Thromb Haemost 8:173-84. 2010
    ..Both types of lesion were sensitive to platelet GPIIb-IIIa and P2Y(12) inhibition, whereas only deep injuries were sensitive to thrombin blockade...
  84. ncbi The F11 receptor (F11R/JAM-A) in atherothrombosis: overexpression of F11R in atherosclerotic plaques
    Anna Babinska
    Department of Anatomy Cell Biology, SUNY Downstate Medical Center, 450 Clarkson Ave, Brooklyn, NY 11203, USA
    Thromb Haemost 97:272-81. 2007
    ....
  85. pmc Using microcontact printing of fibrinogen to control surface-induced platelet adhesion and activation
    Lindsey E Corum
    Department of Bioengineering, University of Utah, 20 South 2030 East, Salt Lake City, Utah 84112, USA
    Langmuir 27:8316-22. 2011
    ..Increasing the overall fibrinogen coverage resulted in higher activation levels defined by key morphological characteristics of the spreading platelet...
  86. ncbi Platelet adhesion to dimeric beta-glycoprotein I under conditions of flow is mediated by at least two receptors: glycoprotein Ibalpha and apolipoprotein E receptor 2'
    M T T Pennings
    Laboratory of Clinical Chemistry and Haematology, University Medical Centre Utrecht, Utrecht, The Netherlands
    J Thromb Haemost 5:369-77. 2007
    ..Dimerized beta2GPI binds to apolipoprotein E receptor 2' (apoER2') on platelets and increases platelet adhesion to collagen under conditions of flow...
  87. ncbi Formation of platelet strings and microthrombi in the presence of ADAMTS-13 inhibitor does not require P-selectin or beta3 integrin
    A K Chauhan
    CBR Institute for Biomedical Research and Department of Pathology, Harvard Medical School, Boston, MA 02115, USA
    J Thromb Haemost 5:583-9. 2007
    ..Recent in vitro studies suggested that P-selectin might anchor these platelet strings to endothelium, but whether the same mechanism exists in vivo remains to be elucidated...
  88. ncbi Two functional active conformations of the integrin {alpha}2{beta}1, depending on activation condition and cell type
    Gerlinde R Van De Walle
    Laboratories for Thrombosis Research, Interdisciplinary Research Centre, Katholieke Universiteit Leuven, Campus Kortrijk, 8500 Kortrijk, Belgium
    J Biol Chem 280:36873-82. 2005
    ....
  89. ncbi Salvianolic acid B inhibits platelet adhesion under conditions of flow by a mechanism involving the collagen receptor alpha2beta1
    Ya Ping Wu
    Institute of Life Sciences, Taishan Medical University, Tai an, China
    Thromb Res 123:298-305. 2008
    ..These combined results indicate that SAB inhibits platelet adhesion to immobilized collagen by interfering with the collagen receptor alpha2beta1...
  90. ncbi Adhesion of human platelets to albumin is synergistically increased by lysophosphatidic acid and adrenaline in a donor-dependent fashion
    Andreas C Eriksson
    Division of Pharmacology, Department of Medicine and Care, Faculty of Health Sciences, Linkoping University, Sweden
    Blood Coagul Fibrinolysis 17:359-68. 2006
    ..We also confirm that synergistic platelet activation achieved with LPA and adrenaline is highly donor dependent...
  91. ncbi Complete deficiency in ADAMTS13 is prothrombotic, but it alone is not sufficient to cause thrombotic thrombocytopenic purpura
    Fumiaki Banno
    National Cardiovascular Center Research Institute, 5 7 1 Fujishirodai, Suita, Osaka 565 8565, Japan
    Blood 107:3161-6. 2006
    ..Alternatively, factors in addition to ADAMTS13 deficiency may be necessary for development of TTP...
  92. ncbi Role of dystrophins and utrophins in platelet adhesion process
    Doris Cerecedo
    Dept Morfologia, Escuela Nacional de Ciencias Biologicas, IPN, D F Mexico
    Br J Haematol 134:83-91. 2006
    ..The simultaneous presence of dystrophin and utrophin complexes indicates complementary structural and signalling mechanisms to the actin network, improving the platelet haemostatic role...
  93. ncbi von Willebrand factor mediates platelet spreading through glycoprotein Ib and alpha(IIb)beta3 in the presence of botrocetin and ristocetin, respectively
    O J T McCarty
    Centre for Cardiovascular Sciences, University of Birmingham, Birmingham, UK
    J Thromb Haemost 4:1367-78. 2006
    ..To accomplish this, VWF binds to two distinct platelet receptors: glycoprotein (GP)Ib-IX-V and integrin alpha(IIb)beta3...
  94. ncbi Congenital platelet disorders: overview of their mechanisms, diagnostic evaluation and treatment
    C P M Hayward
    McMaster University, Hamilton, Ontario, Canada
    Haemophilia 12:128-36. 2006
    ..Presently, there are a number of choices to consider for the management of bleeding symptoms, including menorrhagia. This paper reviews the causes, diagnostic evaluation and therapies for common and rare congenital platelet disorders...
  95. pmc Systemic antithrombotic effects of ADAMTS13
    Anil K Chauhan
    CBR Institute for Biomedical Research and Department of Pathology, Harvard Medical School, Boston, MA 02115, USA
    J Exp Med 203:767-76. 2006
    ..Our findings reveal that ADAMTS13 has a powerful natural antithrombotic activity and recombinant ADAMTS13 could be used as an antithrombotic agent...
  96. ncbi The presence of active von Willebrand factor under various pathological conditions
    Evelyn Groot
    Laboratory for Thrombosis and Haemostasis, Department of Clinical Chemistry and Haematology, University Medical Center Utrecht, The Netherlands
    Curr Opin Hematol 14:284-9. 2007
    ..To highlight mechanisms that regulate the balance between latent and active von Willebrand factor (VWF), and describe pathological conditions leading to increased levels of active VWF...
  97. pmc Platelet adhesion: structural and functional diversity of short dystrophin and utrophins in the formation of dystrophin-associated-protein complexes related to actin dynamics
    Doris Cerecedo
    Departamento Morfología, Escuela Nacional de Ciencias Biologicas, IPN, D F, Mexico
    Thromb Haemost 94:1203-12. 2005
    ..Participation of both Dp71d/Dp71delta110m approximately DAPC and Up400/Up71 approximately DAPC in the biological roles of the platelets is discussed...
  98. ncbi Glucocorticoids inhibit the cerebral microvascular dysfunction associated with sepsis in obese mice
    Vidula Vachharajani
    Critical Care Medicine, Louisiana State University Health Sciences Centre, Shreveport, Louisiana 71130, USA
    Microcirculation 13:477-87. 2006
    ..The objective of this study was to determine whether low-dose steroid therapy, which has proven effective in clinical setting, affords any benefit in the increased morbidity to sepsis in genetically obese (ob/ob) mice...
  99. ncbi A new perfusion chamber to detect platelet adhesion using a small volume of blood
    J J Sixma
    Department of Haematology, University Hospital, Utrecht, The Netherlands
    Thromb Res 92:S43-6. 1998
    ..Platelet adhesion under flow has also been studied with direct observation using this same chamber...
  100. ncbi Measurement of adhesion of human platelets in plasma to protein surfaces in microplates
    Andreas C Eriksson
    Division of Pharmacology, Department of Medicine and Care, Faculty of Health Sciences, Linkoping University, SE 581 85 Linkoping, Sweden
    J Pharmacol Toxicol Methods 52:356-65. 2005
    ..The aim of this work was to develop a method to measure adhesion of platelets in plasma with standard laboratory equipment...
  101. pmc Coordinate interactions of Csk, Src, and Syk kinases with [alpha]IIb[beta]3 initiate integrin signaling to the cytoskeleton
    Achim Obergfell
    Division of Vascular Biology, Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    J Cell Biol 157:265-75. 2002
    ..These studies establish that platelet spreading on fibrinogen requires sequential activation of Src and Syk in proximity to alphaIIbbeta3, thus providing a paradigm for initiation of integrin signaling to the actin cytoskeleton...

Research Grants59

  1. Initiation and Regulation of Platelet Thrombus Formation
    ZAVERIO RUGGERI; Fiscal Year: 2004
    ..The results of the proposed studies will have an impact on public health by providing novel mechanistic information on processes that are central to normal hemostasis and pathological arterial thrombosis. ..
  2. Platelet interactions with vessel wall components
    ZAVERIO RUGGERI; Fiscal Year: 2005
    ..These findings will provide a better definition and characterization of several potential targets for antithrombotic intervention that may yield new treatments for patients at risk of cardiovascular and cerebrovascular events. ..
  3. Mechanisms of Thrombus Instability
    ZAVERIO RUGGERI; Fiscal Year: 2007
    ..These studies will provide novel insights into the unexpected role of leptin in platelet function, and in the formation and stability of thrombi. ..
  4. Structure and function of platelet membrane glycoproteins
    ZAVERIO RUGGERI; Fiscal Year: 2008
    ..abstract_text> ..
  5. Role of von Willebrand Factor in Platelet Thrombus Formation
    Zaverio M Ruggeri; Fiscal Year: 2010
    ..Understanding these different functions requires addressing structural details as well as verification of concepts in intravidal models. This will lead to a better diagnosis and treatment for cardio- and cerebro-vascular diseases. ..
  6. Role of JAM Family in Angiogenesis
    Ulhas P Naik; Fiscal Year: 2010
    ..Further, elucidation of the role of JAM-A in endothelial cell proliferation and migration leading to angiogenesis will open up a mew line of investigation that is important in tumerigenesis and vascular biology. ..
  7. Modulation of integrin function by cytoplasmic signals
    Ulhas Naik; Fiscal Year: 2007
    ..Further, characterization of CIB and its interactions with integrin alphaIIb-beta3 and Plk3 may help in delineating the mechanisms by which cytoplasmic signals modulate integrin function. ..
  8. INTERACTION OF OSTEOPONTIN WITH ADHESION RECEPTORS
    JOEL BENNETT; Fiscal Year: 2002
    ..Our results suggest that it may be possible to inhibit the formation of platelet thrombi in arteries involved by atherosclerosis by preventing the interaction of platelet alphavbeta3 with osteopontin. ..
  9. Blood Systems Biology
    Lawrence Brass; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  10. CHARACTERIZATION OF A NOVEL G PROTEIN IN PLATELETS
    Lawrence Brass; Fiscal Year: 2005
    ..All four of these aims will combine biochemical and pharmacologic approaches with studies on genetically engineered mice lacking selected receptors and G proteins, allowing us to better understand platelet activation in vivo. ..
  11. Proteomic studies of normal and abnormal platelet function
    Lawrence Brass; Fiscal Year: 2007
    ..This includes novel methods that permit quantitation of individual molecules so that changes in levels of expression can be measured. [unreadable] [unreadable] [unreadable]..
  12. FASEB Conf. Proteases in Hemostasis and Vascular Biology
    Lawrence Brass; Fiscal Year: 2005
    ..Tissue factor, platelets and coagulation; 8. Regulators of hemostasis; 9. Protease receptors. The keynote lecture will be on gene therapy for disorders of hemostasis delivered by Dr. Katherine High. ..
  13. Multiplexed HTS of Serine and Cysteine Proteases (RMI)
    Scott Diamond; Fiscal Year: 2005
    ..The use of multiplexed assay on microarrays allows the generation of critical data for the chemical protease interactome. ..
  14. Ischemia Reperfusion Injury in the Diabetic Heart
    Paul McDonagh; Fiscal Year: 2007
    ..The results will improve our understanding of the role of chronic and acute inflammation in I/R injury in the diabetic heart and suggest new, thoughtful approaches to treat this growing problem. [unreadable] [unreadable]..
  15. Mechanobiology of Mesenchymal Stem Cells
    Song Li; Fiscal Year: 2007
    ..g., using BMMSC-derived SMCs to construct tissue-engineered vascular grafts. ..
  16. Molecular Genetics of Integrin Collagen Receptors
    Thomas Kunicki; Fiscal Year: 2007
    ..From a clinical standpoint, these studies will also reveal the impact of these gene differences on risk for adverse events in bleeding disorders such as Von Willebrand Disease. ..
  17. MOLECULAR BASIS OF PLATELET ADHESION
    Miguel Cruz; Fiscal Year: 2008
    ..It may also contribute to the development of an effective assay to detect the activity of the metalloprotease in plasma. It will provide molecular guidance for the generation of reagents to treat arterial thrombosis. ..
  18. Best Practices in Transfusion Medicine for Patients with Sickle Cell Disease
    Theodore Wun; Fiscal Year: 2007
    ..Use of these pathways will improve the care for these patients. (End of Abstract) [unreadable] [unreadable] [unreadable]..
  19. SELECTIN LIGANDS IN LEUKOCYTE BIOLOGY AND INFLAMMATION
    Bruce Furie; Fiscal Year: 2008
    ..Finally, we propose to use the PSGL-1 null mouse to establish whether the absence of PSGL-1 will block or mitigate graft versus host disease following bone marrow transplantation in a mouse model. ..
  20. Characterizing the Phenotype of Staphylococcal Biofilms
    Julia Ross; Fiscal Year: 2008
    ..Completion of these specific aims will provide a rational basis for the design of new therapeutic molecules. ..
  21. Role of Carboxyterminal Hypervariable Region in Rap1b Function
    Gilbert C White; Fiscal Year: 2010
    ..These findings will provide insight into how the body heals bleeding injuries, as well as the underlying cause of blood clotting conditions. ..
  22. Prx1 in malignant progression of prostate cancer
    Candace S Johnson; Fiscal Year: 2010
    ..This study will also provide a soundscientific basis upon which the role ofPrxl canbe elucidatedin prostate cancer, enabling the development of novelprognostic/therapeutic approaches to inhibit its malignant progression. ..
  23. The Penn Center For Molecular Discovery
    Scott Diamond; Fiscal Year: 2008
    ..This project is responsive to the NIH Roadmap objectives to apply both establish HTS/Synthetic methods and emerging next generation technologies developed at the University of Pennsylvania. ..
  24. Optimizing Cardiovascular Devices Thrombogenicity for Eliminating Anticoagulation
    Danny Bluestein; Fiscal Year: 2009
    ..It is envisioned that it will also facilitate the use of these devices for long term therapy by reducing the need for difficult pharmacological management with anticoagulants, which is mandated for most existing devices. ..
  25. Targeting Metastatic Breast Cancer with Human Antibodies
    Brunhilde Felding Habermann; Fiscal Year: 2009
    ..Novel immunoconjugates containing small, highly toxic compounds will be tested for their effects on metastatic breast cancer. If successful, this work will contribute to an improvement of current anti-cancer treatment. ..
  26. S. sanguis Adhesion: Molecular Basis of Specificity
    MARK HERZBERG; Fiscal Year: 2009
    ..abstract_text> ..
  27. Blood Systems Biology
    Scott Diamond; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  28. Actin assembly in platelets & megakaryoctyes
    Charles Abrams; Fiscal Year: 2008
    ..In the second Aim, we will formally address how PIP2 drives reorganization of platelet actin using a combination of biochemistry, murine genetics, and ex vivo platelet adhesion models. ..
  29. Factor lXa-Factor Vllla complexes in blood coagulation
    Bruce Furie; Fiscal Year: 2008
    ..abstract_text> ..
  30. Tissue Engineered Nanofibrous Vascular Graft
    Song Li; Fiscal Year: 2010
    ..The accomplishment of this project will lead to the development of innovative technologies to engineer stem cells and nanofibrous scaffolds for the construction of small TEVGs. ..
  31. Regulation of Outside-In Integrin Signaling in Platelets
    SANFORD SHATTIL; Fiscal Year: 2007
    ..Thus, this line of investigation may lead to identification of new anti-thrombotic drug targets and serve as a paradigm for integrin signaling in other blood cells. [unreadable] [unreadable] [unreadable]..
  32. TRANSCRIPTION FACTOR NF-E2 IN ALPHA IIB BETA 3 SIGNALING
    SANFORD SHATTIL; Fiscal Year: 2004
    ..abstract_text> ..
  33. Proteomic Profiling of Stem Cell Differentiation
    Song Li; Fiscal Year: 2005
    ..The successful accomplishment of the goals will have high impact on stem cell engineering, and provide a rational basis for engineering BMMSC for vascular tissue repair. ..
  34. IVIS 200 Imaging System
    Brunhilde Felding Habermann; Fiscal Year: 2005
    ..abstract_text> ..
  35. DYNAMIC PLATELET - STAPHYLOCOCCAL INTERACTIONS
    Julia Ross; Fiscal Year: 2004
    ..Completion of these specific aims will provide a rational basis for the design of new therapeutic molecules to block specific adhesion events, as well as identify the most important bacterial receptors to target in vaccine development. ..
  36. Role of selectins and selectin ligands in the pathobiol*
    Bruce Furie; Fiscal Year: 2004
    ..To prove the physiologic importance of this pathway, we will attempt to rescue homozygous protein C deficient mice from fatal thrombosis by PSGL-1 deficiency. ..
  37. MOLECULAR BIOLOGY OF COLLAGEN RECEPTORS
    Thomas Kunicki; Fiscal Year: 2004
    ..The successful completion of the proposed studies will lead to a substantial increase in our understanding of the genetics and physiology of the platelet collagen receptor, GPVI. ..
  38. PLATELET-DERIVED NITRIC OXIDE AND HEMOSTASIS
    Jane Freedman; Fiscal Year: 2003
    ....
  39. INTEGRIN CYTODOMAIN ANTI-THROMBOTICS
    David Phillips; Fiscal Year: 2002
    ..In the long term we will develop these novel inhibitors of alphaIIbbeta3 to offer improved therapy for the large population afflicted with ischemic cardiovascular diseases. PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE ..
  40. BIOSYNTHESIS OF BLOOD CLOTTING PROTEINS
    Bruce Furie; Fiscal Year: 2001
    ..Detailed knowledge of these processes will improve our understanding of the biology of these proteins and has potential for improvements in hemophilia therapy. ..
  41. eNOS Polymorphisms, Aging and Platelet Function
    Jane Freedman; Fiscal Year: 2005
    ..We have continued to examine the imoortance of this mutation to the vascular response. ..
  42. Genetic Risk Factors for Portopulmonary Hypertension
    Steven Kawut; Fiscal Year: 2005
    ..In addition, this pilot study could suggest targeted preventative or therapeutic modalities, leading to clinical trials for an "orphan" disease routinely excluded from other research programs and trials of therapy. ..
  43. Neurohormonal Activation in Pulmonary Hypertension
    Steven Kawut; Fiscal Year: 2005
    ..We will formulate prediction rules using neurohormone levels and clinical variables to improve prognostication and management in this disease. ..
  44. Collagen signaling in platelets
    Mark Kahn; Fiscal Year: 2006
    ..These studies will help define the pathogenesis of stroke and myocardial infarction at the molecular level and will lay the foundation for novel therapies to treat these diseases. ..
  45. INTRAVITAL VIDEO-RATE CONFOCAL MICROSCOPE
    Bruce Furie; Fiscal Year: 2001
    ..The BioRad RTS2000 real-time laser confocal system, fitted to a Nikon E600FN microscope, will provide a group of investigators with instrumentation to significantly advance their fields. ..
  46. Oral Epithelial Cells: Innate Immune "Gatekeeper" of HIV
    MARK HERZBERG; Fiscal Year: 2006
    ..Innate immune factor-related genes may prove to be novel targets to prevent mucosal HIV. ..
  47. INNATE INTRAEPITHELIAL DEFENSE AGAINST ORAL INFECTION
    MARK HERZBERG; Fiscal Year: 2006
    ..abstract_text> ..
  48. Integrin Activation in Breast Cancer Metastasis
    Brunhilde Felding Habermann; Fiscal Year: 2006
    ....
  49. Controlled Drug Delivery to Colon Tumors Via Ultrasound
    WILLIAM PITT; Fiscal Year: 2006
    ..The research outcomes will prove hypotheses, which will become the basis for understanding and exploiting this therapy in the treatment of localized cancers, hopefully paving the way for clinical application. ..
  50. Regulation of vascular development and function by LKLF
    Mark L Kahn; Fiscal Year: 2010
    ..Understanding the mechanism by which LKLF regulates genetic responses in the developing and mature vasculature is expected to provide new insights into the pathogenesis and treatment of human vascular diseases. ..
  51. YOUTH TAKE HEART (PHASE I & II)
    Buddy Ratner; Fiscal Year: 2007
    ..Youth Take Heart will help enhance the cardiovascular health and quality of life for numerous students through the teaching of medical management, prevention, and control of risk factors for heart disease. ..
  52. GLUCOCORTICOIDS & VITAMIN D: ROLE IN ANTI-TUMOR EFFECTS
    Candace Johnson; Fiscal Year: 2009
    ..abstract_text> ..
  53. Reaction and Transport Dynamics in Human Blood
    Scott Diamond; Fiscal Year: 2009
    ..Overall, these studies seek to provide fundamental insight into cell-cell interacitions and coagulation biochemistry that occur under flow. ..
  54. Platelet Liposomal Drug Delivery--Thrombosis /Restenosis
    Shuwu Wang; Fiscal Year: 2006
    ..The targeted liposomal system proposed could reduce the costs in both treatment areas with a 5% reduction in revascularizations alone leading to >$60 million annual medical cost savings. ..
  55. 1st International Symposium on Wound Healing and Technology (WHAT I)
    Buddy Ratner; Fiscal Year: 2006
    ..Communication and discussion of these problems and new ideas for how we can address them are central to this meeting. [unreadable] [unreadable] [unreadable]..
  56. Engineered Cardiac Morphogenesis-Stem Cells and Scaffolds
    Buddy D Ratner; Fiscal Year: 2010
    ..This comprehensive approach to heart muscle repair will be implemented by a collaborative, multidisciplinary team closely integrating engineers, scientists and clinicians. ..
  57. Training in Engineered Biomaterials
    Buddy Ratner; Fiscal Year: 2007
    ..Trainees will participate in the training program for a period of 2 years. ..
  58. Biomimetic Coatings for Small Diameter Vascular Grafts
    Shuwu Wang; Fiscal Year: 2004
    ..We anticipate that this project will lead to highly effective biomimetic materials that facilitate shear stable modification of small diameter vascular prostheses that demonstrate improved blood compatibility. ..
  59. High Intensity Ultrasound in Gastroenterology
    JOO HWANG; Fiscal Year: 2003
    ..It is anticipated that this work will help to gain a better understanding of the mechanisms of HIFU and its potential role for other GI applications such as tumor therapy. ..