membrane potentials

Summary

Summary: Ratio of inside versus outside concentration of potassium, sodium, chloride and other ions in diffusible tissues or cells. Also called transmembrane and resting potentials, they are measured by recording electrophysiologic responses in voltage-dependent ionic channels of (e.g.) nerve, muscle and blood cells as well as artificial membranes.

Top Publications

  1. ncbi Prevention of apoptosis by Bcl-2: release of cytochrome c from mitochondria blocked
    J Yang
    Department of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322, USA
    Science 275:1129-32. 1997
  2. ncbi Noxious cold ion channel TRPA1 is activated by pungent compounds and bradykinin
    Michael Bandell
    Genomics Institute of the Novartis Research Foundation, San Diego, CA 92121, USA
    Neuron 41:849-57. 2004
  3. doi Molecular background of leak K+ currents: two-pore domain potassium channels
    Peter Enyedi
    Department of Physiology, Semmelweis University, Budapest, Hungary
    Physiol Rev 90:559-605. 2010
  4. ncbi A model for human ventricular tissue
    K H W J ten Tusscher
    Department of Theoretical Biology, Utrecht University, 3584 CH Utrecht, The Netherlands
    Am J Physiol Heart Circ Physiol 286:H1573-89. 2004
  5. ncbi Glutamate exocytosis from astrocytes controls synaptic strength
    Pascal Jourdain
    Department of Cell Biology and Morphology, University of Lausanne, Rue du Bugnon 9, 1005 Lausanne, Switzerland
    Nat Neurosci 10:331-9. 2007
  6. ncbi A mechanistic link between an inherited and an acquired cardiac arrhythmia: HERG encodes the IKr potassium channel
    M C Sanguinetti
    Eccles Program in Human Molecular Biology and Genetics, University of Utah Health Sciences Center, Salt Lake City 84112, USA
    Cell 81:299-307. 1995
  7. ncbi ANKTM1, a TRP-like channel expressed in nociceptive neurons, is activated by cold temperatures
    Gina M Story
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Cell 112:819-29. 2003
  8. ncbi Small-conductance, calcium-activated potassium channels from mammalian brain
    M Kohler
    Vollum Institute, L 474, Oregon Health Sciences University, 3181 Southwest Sam Jackson Road, Portland, OR 97201, USA J Maylie, Department of Obstetrics and Gyne
    Science 273:1709-14. 1996
  9. ncbi Excitatory actions of GABA in the cortex
    Allan T Gulledge
    Division of Neuroscience, John Curtin School of Medical Research, Australian National University, Mills Road, Canberra, ACT 0200, Australia
    Neuron 37:299-309. 2003
  10. ncbi HERG, a human inward rectifier in the voltage-gated potassium channel family
    M C Trudeau
    Department of Physiology, University of Wisconsin Medical School, Madison 53706, USA
    Science 269:92-5. 1995

Research Grants

  1. DYNAMICS OF MEMBRANE ORGANIZATION AT NODE OF RANVIER
    Mark Ellisman; Fiscal Year: 1993
  2. DYNAMICS OF MEMBRANE ORGANIZATION AT NODE OF RANVIER
    Mark Ellisman; Fiscal Year: 1992
  3. DYNAMICS OF MEMBRANE ORGANIZATION AT NODE OF RANVIER
    Mark Ellisman; Fiscal Year: 1991
  4. EFFECT OF OXIDANT STRESS ON ALVEOLAR MACROPHAGE FUNCTION
    HENRY FORMAN; Fiscal Year: 1992
  5. EFFECT OF OXIDANT STRESS ON ALVEOLAR MACROPHAGE FUNCTION
    HENRY FORMAN; Fiscal Year: 1991
  6. CHEMOTRANSDUCTION IN THE CAROTID BODY
    Robert Fitzgerald; Fiscal Year: 1999
  7. CHEMOTRANSDUCTION IN THE CAROTID BODY
    Robert Fitzgerald; Fiscal Year: 2000
  8. CHEMOTRANSDUCTION IN THE CAROTID BODY
    Robert Fitzgerald; Fiscal Year: 2001
  9. EXOCYTIC INSERTION OF POTASSIUM CHANNELS IN MEMBRANES
    William Moran; Fiscal Year: 2001
  10. PROCESSING AND DEGRADATION OF PROTEOLIPID PROTEIN
    Robert P Skoff; Fiscal Year: 2012

Detail Information

Publications277 found, 100 shown here

  1. ncbi Prevention of apoptosis by Bcl-2: release of cytochrome c from mitochondria blocked
    J Yang
    Department of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322, USA
    Science 275:1129-32. 1997
    ..Overexpression of Bcl-2 prevented the efflux of cytochrome c from the mitochondria and the initiation of apoptosis. Thus, one possible role of Bcl-2 in prevention of apoptosis is to block cytochrome c release from mitochondria...
  2. ncbi Noxious cold ion channel TRPA1 is activated by pungent compounds and bradykinin
    Michael Bandell
    Genomics Institute of the Novartis Research Foundation, San Diego, CA 92121, USA
    Neuron 41:849-57. 2004
    ..Collectively, these data demonstrate that TRPA1 activation elicits a painful sensation and provide a potential molecular model for why noxious cold can paradoxically be perceived as burning pain...
  3. doi Molecular background of leak K+ currents: two-pore domain potassium channels
    Peter Enyedi
    Department of Physiology, Semmelweis University, Budapest, Hungary
    Physiol Rev 90:559-605. 2010
    ..In this review, we focus on the physiological roles of K(2P) channels in the most extensively investigated cell types, with special emphasis on the molecular mechanisms of channel regulation...
  4. ncbi A model for human ventricular tissue
    K H W J ten Tusscher
    Department of Theoretical Biology, Utrecht University, 3584 CH Utrecht, The Netherlands
    Am J Physiol Heart Circ Physiol 286:H1573-89. 2004
    ..We conclude that the proposed model reproduces a variety of electrophysiological behaviors and provides a basis for studies of reentrant arrhythmias in human ventricular tissue...
  5. ncbi Glutamate exocytosis from astrocytes controls synaptic strength
    Pascal Jourdain
    Department of Cell Biology and Morphology, University of Lausanne, Rue du Bugnon 9, 1005 Lausanne, Switzerland
    Nat Neurosci 10:331-9. 2007
    ..Thus, we provide the first combined functional and ultrastructural evidence for a physiological control of synaptic activity via exocytosis of glutamate from astrocytes...
  6. ncbi A mechanistic link between an inherited and an acquired cardiac arrhythmia: HERG encodes the IKr potassium channel
    M C Sanguinetti
    Eccles Program in Human Molecular Biology and Genetics, University of Utah Health Sciences Center, Salt Lake City 84112, USA
    Cell 81:299-307. 1995
    ..Since block of IKr is a known mechanism for drug-induced cardiac arrhythmias, the finding that HERG encodes IKr channels provides a mechanistic link between certain forms of inherited and acquired LQT...
  7. ncbi ANKTM1, a TRP-like channel expressed in nociceptive neurons, is activated by cold temperatures
    Gina M Story
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Cell 112:819-29. 2003
    ..Consistent with the expression of ANKTM1, we identify noxious cold-sensitive sensory neurons that also respond to capsaicin but not to menthol...
  8. ncbi Small-conductance, calcium-activated potassium channels from mammalian brain
    M Kohler
    Vollum Institute, L 474, Oregon Health Sciences University, 3181 Southwest Sam Jackson Road, Portland, OR 97201, USA J Maylie, Department of Obstetrics and Gyne
    Science 273:1709-14. 1996
    ....
  9. ncbi Excitatory actions of GABA in the cortex
    Allan T Gulledge
    Division of Neuroscience, John Curtin School of Medical Research, Australian National University, Mills Road, Canberra, ACT 0200, Australia
    Neuron 37:299-309. 2003
    ..Our findings demonstrate that under certain circumstances GABA will have an excitatory role in synaptic integration in the cortex...
  10. ncbi HERG, a human inward rectifier in the voltage-gated potassium channel family
    M C Trudeau
    Department of Physiology, University of Wisconsin Medical School, Madison 53706, USA
    Science 269:92-5. 1995
    ..Because mutations in HERG cause a form of long-QT syndrome, these properties of HERG channel function may be critical to the maintenance of normal cardiac rhythmicity...
  11. ncbi The inward rectification mechanism of the HERG cardiac potassium channel
    P L Smith
    Department of Neurobiology, Harvard Medical School, Boston, MA 02114 2698, USA
    Nature 379:833-6. 1996
    ..The characteristics of this gating suggest a specific role for this channel in the normal suppression of arrhythmias...
  12. ncbi Cellular and network mechanisms of slow oscillatory activity (<1 Hz) and wave propagations in a cortical network model
    Albert Compte
    Volen Center for Complex Systems, Brandeis University, Waltham, Massachusetts 02454, USA
    J Neurophysiol 89:2707-25. 2003
    ..Finally, we show with this model that various known physiological effects of neuromodulation can switch the network to tonic firing, thus simulating a transition to the waking state...
  13. pmc Primary structure and functional expression of the human cardiac tetrodotoxin-insensitive voltage-dependent sodium channel
    M E Gellens
    Department of Medicine, University of Pennsylvania, Philadelphia 19104
    Proc Natl Acad Sci U S A 89:554-8. 1992
    ..7 microM). hH1 is also resistant to mu-conotoxin but sensitive to block by therapeutic concentrations of lidocaine in a use-dependent manner...
  14. pmc Effect of VX-770 in persons with cystic fibrosis and the G551D-CFTR mutation
    Frank J Accurso
    University of Colorado Denver and Children s Hospital, Aurora, CO 80045, USA
    N Engl J Med 363:1991-2003. 2010
    ..VX-770, a CFTR potentiator, has been shown to increase the activity of wild-type and defective cell-surface CFTR in vitro...
  15. ncbi Inhibitory postsynaptic potentials carry synchronized frequency information in active cortical networks
    Andrea Hasenstaub
    Department of Neurobiology, Kavli Institute of Neuroscience, Yale University School of Medicine, New Haven, Connecticut 06510, USA
    Neuron 47:423-35. 2005
    ..Our results support the hypothesis that inhibitory networks are largely responsible for the dissemination of higher-frequency activity in cortex...
  16. ncbi Pannexin membrane channels are mechanosensitive conduits for ATP
    Li Bao
    Department of Physiology and Biophysics, University of Miami School of Medicine, 1600 NW 10th Ave, Miami, FL 33136, USA
    FEBS Lett 572:65-8. 2004
    ..They have a wide expression spectrum, they are of large conductance and permeant for ATP, and they are mechanosensitive. Hence, pannexins are candidates for the release of ATP to the extracellular space upon mechanical stress...
  17. ncbi Functional effects of two voltage-gated sodium channel mutations that cause generalized epilepsy with febrile seizures plus type 2
    J Spampanato
    Department of Microbiology and Molecular Genetics, University of California, Irvine, California 92697-4025, USA
    J Neurosci 21:7481-90. 2001
    ....
  18. ncbi K+ channels and the microglial respiratory burst
    R Khanna
    Division of Cellular and Molecular Biology, Toronto Western Research Institute, University Health Network, Toronto, Ontario, Canada
    Am J Physiol Cell Physiol 280:C796-806. 2001
    ..The respiratory burst was markedly inhibited by blockers of SK2 (apamin) and SK4 channels (clotrimazole and charybdotoxin), and to a lesser extent, by the potent Kv1.3 blocker agitoxin-2...
  19. ncbi Time course and nerve growth factor dependence of inflammation-induced alterations in electrophysiological membrane properties in nociceptive primary afferent neurons
    L Djouhri
    Department of Physiology, University of Bristol, Medical School, University Walk, Bristol BS8 TD, United Kingdom
    J Neurosci 21:8722-33. 2001
    ....
  20. ncbi Natural waking and sleep states: a view from inside neocortical neurons
    M Steriade
    Laboratoire de Neurophysiologie, Faculte de Medicine, Universite Laval, Quebec G1K 7P4, Canada
    J Neurophysiol 85:1969-85. 2001
    ..In view of the high firing rates in the functionally disconnected state of slow-wave sleep, we suggest that neocortical neurons are engaged in processing internally generated signals...
  21. pmc Properties of hyperpolarization-activated pacemaker current defined by coassembly of HCN1 and HCN2 subunits and basal modulation by cyclic nucleotide
    S Chen
    Department of Pharmacology, ColumbiaUniversity, New York, New York 10032, USA
    J Gen Physiol 117:491-504. 2001
    ..Finally, differences in Ih channel properties recorded in cell-free patches versus intact oocytes are shown to be due, in part, to modulation of Ih by basal levels of cAMP in intact cells...
  22. ncbi Spontaneous astrocytic Ca2+ oscillations in situ drive NMDAR-mediated neuronal excitation
    H R Parri
    School of Biosciences, Cardiff University, Museum Avenue, PO Box 911, Cardiff, CF10 3US, Wales, UK
    Nat Neurosci 4:803-12. 2001
    ..These findings show that astrocytes in situ can act as a primary source for generating neuronal activity in the mammalian central nervous system...
  23. ncbi The mitochondrial permeability transition initiates autophagy in rat hepatocytes
    S P Elmore
    Department of Cell and Developmental Biology and Curriculum in Toxicology, University of North Carolina, Chapel Hill, NC 27599, USA
    FASEB J 15:2286-7. 2001
    ..In conclusion, the MPT initiates mitochondrial depolarization after autophagic stimulation and the subsequent sequestration of mitochondria into autophagosomes...
  24. pmc Transmembrane voltage potential controls embryonic eye patterning in Xenopus laevis
    Vaibhav P Pai
    Department of Biology and Tufts Center for Regenerative and Developmental Biology, Tufts University, Medford, MA 02155, USA
    Development 139:313-23. 2012
    ..Learning to control bioelectric initiators of organogenesis offers significant insight into birth defects that affect the eye and might have significant implications for regenerative approaches to ocular diseases...
  25. pmc The S4-S5 linker couples voltage sensing and activation of pacemaker channels
    J Chen
    Department of Medicine, Division of Cardiology, Eccles Program in Human Molecular Biology and Genetics, University of Utah, Eccles Institute of Human Genetics, 15 N 2030 E, Room 4220, Salt Lake City, UT 84112, USA
    Proc Natl Acad Sci U S A 98:11277-82. 2001
    ..Our findings also suggest that opening of HCN and related channels corresponds to activation of a gate located near the inner pore, rather than recovery of channels from a C-type inactivated state...
  26. doi Direct measurement of somatic voltage clamp errors in central neurons
    Stephen R Williams
    Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, UK
    Nat Neurosci 11:790-8. 2008
    ..These errors question the use of the somatic voltage clamp as a quantitative tool in dendritic neurons...
  27. ncbi Pharmacological properties of homomeric and heteromeric pannexin hemichannels expressed in Xenopus oocytes
    Roberto Bruzzone
    Department of Neuroscience, Institut Pasteur, Paris, France
    J Neurochem 92:1033-43. 2005
    ..The opposite was true in the case of connexin46 (Cx46), thus indicating that gap junction blockers are able to selectively modulate pannexin and connexin channels...
  28. doi HCN channels: structure, cellular regulation and physiological function
    C Wahl-Schott
    Center for Integrated Protein Science CIPSM and Zentrum für Pharmaforschung, Department Pharmazie, Pharmakologie für Naturwissenschaften, Ludwig Maximilians Universitat Munchen, Butenandtstr 5 13, 81377 Munchen, Germany
    Cell Mol Life Sci 66:470-94. 2009
    ..In this review we give an overview on structure, function and regulation of HCN channels. Particular emphasis will be laid on the complex roles of these channels for neuronal function and cardiac rhythmicity...
  29. pmc TRIP8b splice variants form a family of auxiliary subunits that regulate gating and trafficking of HCN channels in the brain
    Bina Santoro
    Department of Neuroscience, Columbia University, 1051 Riverside Drive, New York, NY 10032, USA
    Neuron 62:802-13. 2009
    ..TRIP8b thus functions as an auxiliary subunit that provides a mechanism for the dynamic regulation of HCN1 channel expression and function...
  30. pmc Measurement of mitochondrial membrane potential using fluorescent rhodamine derivatives
    R C Scaduto
    Department of Cellular and Molecular Physiology, College of Medicine, The Pennsylvania State University, Hershey, Pennsylvania 17033 USA
    Biophys J 76:469-77. 1999
    ....
  31. ncbi The size of gating charge in wild-type and mutant Shaker potassium channels
    N E Schoppa
    Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, CT 06510
    Science 255:1712-5. 1992
    ..These results can be explained by a model for channel activation in which the equilibria of voltage-dependent steps are altered in the mutant channels...
  32. ncbi Role of membrane potential in the regulation of cell proliferation and differentiation
    Sarah Sundelacruz
    Department of Biomedical Engineering, Tufts University, 4 Colby St, Medford, MA 02155, USA
    Stem Cell Rev 5:231-46. 2009
    ....
  33. pmc Bioelectric mechanisms in regeneration: Unique aspects and future perspectives
    Michael Levin
    Tufts Center for Regenerative and Developmental Biology, Biology Department, Tufts University, Medford, MA 02155, USA
    Semin Cell Dev Biol 20:543-56. 2009
    ..This chapter reviews the unique properties of bioelectric signaling, surveys molecular strategies and reagents for its investigation, and discusses the opportunities made available for regenerative medicine...
  34. ncbi Activation of pannexin 1 channels by ATP through P2Y receptors and by cytoplasmic calcium
    Silviu Locovei
    Department of Physiology and Biophysics, University of Miami, School of Medicine, P O Box 016430, 1600 NW 10th Avenue, Miami, FL 33136, USA
    FEBS Lett 580:239-44. 2006
    ..Based on its properties, including ATP permeability, pannexin 1 may be involved in both initiation and propagation of calcium waves...
  35. pmc Pore region of TRPV3 ion channel is specifically required for heat activation
    JORG GRANDL
    Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    Nat Neurosci 11:1007-13. 2008
    ..These findings demonstrate that the temperature sensitivity of TRPV3 is separable from all other known activation mechanisms and implicate a specific region in temperature sensing...
  36. ncbi Heteromeric NMDA receptors: molecular and functional distinction of subtypes
    H Monyer
    Center for Molecular Biology, University of Heidelberg, Germany
    Science 256:1217-21. 1992
    ..Such heteromeric NMDA receptor subtypes may exist in neurons, since NR1 messenger RNA is synthesized throughout the mature rat brain, while NR2 messenger RNA show a differential distribution...
  37. ncbi Expanded CUG repeats trigger aberrant splicing of ClC-1 chloride channel pre-mRNA and hyperexcitability of skeletal muscle in myotonic dystrophy
    Ami Mankodi
    Department of Neurology, School of Medicine and Dentistry, University of Rochester, Box 673, 601 Elmwood Avenue, New York 14642, USA
    Mol Cell 10:35-44. 2002
    ..We propose that a transdominant effect of mutant RNA on RNA processing leads to chloride channelopathy and membrane hyperexcitability in DM...
  38. pmc Bioelectric controls of cell proliferation: ion channels, membrane voltage and the cell cycle
    Douglas J Blackiston
    Biology Department, and Center for Regenerative and Developmental Biology, Tufts University, Medford, MA, USA
    Cell Cycle 8:3519-28. 2009
    ..New advances in genomics and the development of in vivo biophysical techniques suggest exciting opportunities for molecular medicine, bioengineering and regenerative approaches to human health...
  39. ncbi Ischemia opens neuronal gap junction hemichannels
    Roger J Thompson
    Department of Psychiatry and Brain Research Centre, University of British Columbia, Vancouver, British Columbia V6T 2B5, Canada
    Science 312:924-7. 2006
    ..Both the current and dye flux were blocked by inhibitors of hemichannels. Therefore, hemichannel opening contributes to the profound ionic dysregulation during stroke and may be a ubiquitous component of ischemic neuronal death...
  40. ncbi Expression modification of uncoupling proteins and MnSOD in retinal endothelial cells and pericytes induced by high glucose: the role of reactive oxygen species in diabetic retinopathy
    Yan Cui
    Department of Ophthalmology, Shanghai First People s Hospital, 85 Wu Jin Road, Shanghai 200080, PR China
    Exp Eye Res 83:807-16. 2006
    ..Those proteins related to antioxidation mechanism, such as MnSOD and UCPs, could exert compensative action to a certain extent. This compensative action was insufficient when the glucose concentration was too high...
  41. ncbi Synaptic basis for intense thalamocortical activation of feedforward inhibitory cells in neocortex
    Scott J Cruikshank
    Department of Neuroscience, Division of Biology and Medicine, Box G LN, Brown University, Providence, Rhode Island 02912, USA
    Nat Neurosci 10:462-8. 2007
    ..These results demonstrate the importance of selective synaptic targeting and precise timing in the initial stages of neocortical processing...
  42. pmc OPA1 processing controls mitochondrial fusion and is regulated by mRNA splicing, membrane potential, and Yme1L
    Zhiyin Song
    Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA
    J Cell Biol 178:749-55. 2007
    ..Cleavage at S2 is regulated by the i-AAA protease Yme1L. Our results suggest that mammalian cells have multiple pathways to control mitochondrial fusion through regulation of the spectrum of OPA1 isoforms...
  43. pmc Seizure-induced plasticity of h channels in entorhinal cortical layer III pyramidal neurons
    Mala M Shah
    Division of Neuroscience, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
    Neuron 44:495-508. 2004
    ..dendritic excitability was enhanced, resulting in increased neuronal firing despite hyperpolarized membrane potentials. The loss of I(h) and the increased neuronal excitability persisted for 1 week following seizures...
  44. ncbi Alternative to hand-tuning conductance-based models: construction and analysis of databases of model neurons
    Astrid A Prinz
    Volen Center and Biology Department, Brandeis University, Waltham, Massachusetts 02454, USA
    J Neurophysiol 90:3998-4015. 2003
    ..In all cases, database searches can provide insight into how neuronal and network properties depend on the values of the parameters in the models...
  45. ncbi An extrasynaptic GABAA receptor mediates tonic inhibition in thalamic VB neurons
    Fan Jia
    CV Starr Laboratory for Molecular Neuropharmacology, Department of Anesthesiology, Weill Medical College, Cornell University, New York, NY 10021, USA
    J Neurophysiol 94:4491-501. 2005
    ..In view of the critical role of thalamic neurons in the generation of oscillatory activity associated with sleep, these receptors may represent a principal site of action for the novel hypnotic agent gaboxadol...
  46. ncbi Variable channel expression in identified single and electrically coupled neurons in different animals
    David J Schulz
    Volen Center and Biology Department, Brandeis University, Waltham, Massachusetts 02454, USA
    Nat Neurosci 9:356-62. 2006
    ....
  47. ncbi Properties of basal dendrites of layer 5 pyramidal neurons: a direct patch-clamp recording study
    Thomas Nevian
    Department of Physiology, University of Berne, Buhlplatz 5, 3012 Berne, Switzerland
    Nat Neurosci 10:206-14. 2007
    ....
  48. ncbi Conditional knock-out of Kir4.1 leads to glial membrane depolarization, inhibition of potassium and glutamate uptake, and enhanced short-term synaptic potentiation
    Biljana Djukic
    Department of Pharmacology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    J Neurosci 27:11354-65. 2007
    ..1 cKO hippocampus. Our findings implicate a role for glial Kir4.1 channel subunit in the modulation of synaptic strength...
  49. ncbi Protective role of ATP-sensitive potassium channels in hypoxia-induced generalized seizure
    K Yamada
    Department of Physiology, Akita University School of Medicine, Hondo, Akita 010 8543, Japan
    Science 292:1543-6. 2001
    ..K(ATP) channels exert a depressant effect on SNr neuronal activity during hypoxia and may be involved in the nigral protection mechanism against generalized seizures...
  50. ncbi The role of dynamin-related protein 1, a mediator of mitochondrial fission, in apoptosis
    S Frank
    Surgical Neurology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Behesda, Maryland 20892, USA
    Dev Cell 1:515-25. 2001
    ..Remarkably, inhibition of Drp1 blocks cell death, implicating mitochondrial fission as an important step in apoptosis...
  51. pmc An electrostatic potassium channel opener targeting the final voltage sensor transition
    Sara I Börjesson
    Department of Clinical and Experimental Medicine, Division of Cell Biology, Linkoping University, Sweden
    J Gen Physiol 137:563-77. 2011
    ..The identified site and the pharmacological mechanism will potentially be very useful in future drug design of small-molecule compounds specifically targeting neuronal and cardiac excitability...
  52. pmc Charge substitution for a deep-pore residue reveals structural dynamics during BK channel gating
    Xixi Chen
    Section of Neurobiology and Center for Learning and Memory, The University of Texas at Austin, Austin, TX 78712, USA
    J Gen Physiol 138:137-54. 2011
    ..With models based on known structures of related channels, we postulate a dynamic rearrangement of the deep-pore region during BK channel opening/closing, which involves a change of the degree of pore exposure for 314...
  53. pmc Synapses with short-term plasticity are optimal estimators of presynaptic membrane potentials
    Jean Pascal Pfister
    Computational and Biological Learning Lab, Department of Engineering, University of Cambridge, Cambridge, UK
    Nat Neurosci 13:1271-5. 2010
    ..Our analysis provides a unifying account of a powerful, but puzzling, form of plasticity...
  54. ncbi KCNQ5, a novel potassium channel broadly expressed in brain, mediates M-type currents
    B C Schroeder
    Zentrum fur Molekulare Neurobiologie Hamburg, Hamburg University, Martinistrasse 85, D 20246 Hamburg, Germany
    J Biol Chem 275:24089-95. 2000
    ..A KCNQ5 splice variant found in skeletal muscle displays altered gating kinetics. This indicates a molecular diversity of channels yielding M-type currents and suggests a role for KCNQ5 in the regulation of neuronal excitability...
  55. pmc Time constants of h current in layer ii stellate cells differ along the dorsal to ventral axis of medial entorhinal cortex
    Lisa M Giocomo
    Center for Memory and Brain, Program in Neuroscience and Department of Psychology, Boston University, Boston, Massachusetts 02215, USA
    J Neurosci 28:9414-25. 2008
    ..Differences in h current could underlie differences in membrane potential oscillation properties and contribute to grid cell periodicity along the dorsal-ventral axis of medial entorhinal cortex...
  56. pmc Differential effects of beta 1 and beta 2 subunits on BK channel activity
    Patricio Orio
    Centro de Estudios Cientificos, Valdivia, Chile
    J Gen Physiol 125:395-411. 2005
    ..All allosteric coupling factors need to be increased in order to explain the observed effects when the alpha subunit is coexpressed with the beta2IR subunit...
  57. pmc A novel nicotinic acetylcholine receptor subtype in basal forebrain cholinergic neurons with high sensitivity to amyloid peptides
    Qiang Liu
    Divisions of Neurology, Barrow Neurological Institute, St Joseph s Hospital and Medical Center, Phoenix, Arizona 85013 4496, USA
    J Neurosci 29:918-29. 2009
    ....
  58. ncbi H+ pump-dependent changes in membrane voltage are an early mechanism necessary and sufficient to induce Xenopus tail regeneration
    Dany S Adams
    Center for Regenerative and Developmental Biology, Forsyth Institute, and Developmental Biology Department, Harvard School of Dental Medicine, 140 The Fenway, Boston, MA 02115, USA
    Development 134:1323-35. 2007
    ..Control of H(+) flows represents a very important new modality that, together with traditional biochemical approaches, may eventually allow augmentation of regeneration for therapeutic applications...
  59. ncbi Kv1 currents mediate a gradient of principal neuron excitability across the tonotopic axis in the rat lateral superior olive
    Margaret Barnes-Davies
    Department of Cell Physiology and Pharmacology, University of Leicester, PO Box 138, Leicester LE1 9HN, UK
    Eur J Neurosci 19:325-33. 2004
    ..1 immunolabelling. We conclude that Kv1 channels underlie the gradient of LSO principal neuron firing properties. The properties of single-spiking neurons would render them particularly suited to preserving timing information...
  60. ncbi Efficacy of MitoTracker Green and CMXrosamine to measure changes in mitochondrial membrane potentials in living cells and tissues
    W Pendergrass
    Department of Pathology 357470, University of Washington Medical School, Seattle, WA 98195, USA
    Cytometry A 61:162-9. 2004
    ..Because MTG was recently reported to be sensitive to MMP, we have reevaluated the effects of loss of MMP on MTG and CMXRos fluorescence, using both flow cytometry and laser scanning confocal microscopy (LSCM)...
  61. ncbi Changes in the expression of tetrodotoxin-sensitive sodium channels within dorsal root ganglia neurons in inflammatory pain
    Joel A Black
    Department of Neurology and Paralyzes Veterans of America, Eastern Paralyzed Veterans Association Neuroscience Research Center, Yale University School of Medicine, New Haven CT 06510, USA
    Pain 108:237-47. 2004
    ....
  62. ncbi Cellular expression and functional characterization of four hyperpolarization-activated pacemaker channels in cardiac and neuronal tissues
    S Moosmang
    , , Germany
    Eur J Biochem 268:1646-52. 2001
    ..Our results suggest that the heterogeneity of native I(h) currents is generated, at least in part, by the tissue-specific expression of HCN channel genes...
  63. pmc The spine neck filters membrane potentials
    Roberto Araya
    Howard Hughes Medical Institute and Department of Biological Sciences, Columbia University, New York, NY 10027, USA
    Proc Natl Acad Sci U S A 103:17961-6. 2006
    ..We conclude that the spine neck plays an electrical role in the transmission of membrane potentials, isolating synapses electrically.
  64. ncbi TREK-2 (K2P10.1) and TRESK (K2P18.1) are major background K+ channels in dorsal root ganglion neurons
    Dawon Kang
    Department of Physiology and Biophysics, Rosalind Franklin University of Medicine and Science, Chicago Medical School, 3333 Green Bay Road, North Chicago, IL 60064, USA
    Am J Physiol Cell Physiol 291:C138-46. 2006
    ..As TREKs and TRESK are targets of modulation by receptor agonists, they are likely to play an active role in the regulation of excitability in DRG neurons...
  65. ncbi Voltage-sensing mechanism is conserved among ion channels gated by opposite voltages
    Roope Mannikko
    Department of Neuroscience, The Nobel Institute for Neurophysiology, Karolinska Institutet, SE 171 77 Stockholm, Sweden
    Nature 419:837-41. 2002
    ....
  66. pmc Cell-attached measurements of the firing threshold of rat hippocampal neurones
    D Fricker
    Laboratoire de Neurobiologie Cellulaire, INSERM U261, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris, France
    J Physiol 517:791-804. 1999
    ..At potentials close to threshold, the rate of inactivation of Na+ and K+ followed a double exponential time course, such that Na+ currents were 62 % inactivated and K+ currents were 63 % inactivated within 15 ms...
  67. ncbi Channelrhodopsin-1: a light-gated proton channel in green algae
    Georg Nagel
    Max Planck Institut fur Biophysik, Kennedyallee 70, 60596 Frankfurt am Main, Germany
    Science 296:2395-8. 2002
    ..We suggest that Channelrhodopsins are involved in phototaxis of green algae...
  68. ncbi Intracellular acidosis enhances the excitability of working muscle
    Thomas H Pedersen
    Department of Physiology, University of Aarhus, DK 8000, Denmark
    Science 305:1144-7. 2004
    ..These results implicate chloride ion channels in muscle function and emphasize that intracellular acidosis of muscle has protective effects during muscle fatigue...
  69. ncbi The two-pore domain K+ channel, TRESK, is activated by the cytoplasmic calcium signal through calcineurin
    Gábor Czirják
    Department of Physiology and Laboratory of Neuromorphology, Semmelweis University, H 1444 Budapest, Hungary
    J Biol Chem 279:18550-8. 2004
    ....
  70. ncbi State-dependent calcium signaling in dendritic spines of striatal medium spiny neurons
    Adam G Carter
    Department of Neurobiology, Harvard Medical School, 220 Longwood Avenue, Boston, MA 02115, USA
    Neuron 44:483-93. 2004
    ..By altering the sources, amplitude, and kinetics of spine Ca signals, state transitions may gate synaptic plasticity and gene expression in MSNs...
  71. pmc A role for hydrophobic residues in the voltage-dependent gating of Shaker K+ channels
    K McCormack
    Division of Biology 216 76, California Institute of Technology, Pasadena 91125
    Proc Natl Acad Sci U S A 88:2931-5. 1991
    ..Our results suggest that the leucines mediate interactions that play an important role in the transduction of charge movement into channel opening and closing...
  72. ncbi Theta-frequency bursting and resonance in cerebellar granule cells: experimental evidence and modeling of a slow k+-dependent mechanism
    E D'Angelo
    Department of Molecular Cellular Physiology and Instituto Nazionale per la Fisica della Materia, University of Pavia, I 27100 Pavia, Italy
    J Neurosci 21:759-70. 2001
    ..Theta-frequency bursting and resonance in granule cells may play an important role in determining synchronization, rhythmicity, and learning in the cerebellum...
  73. ncbi Asymmetries in H+/K+-ATPase and cell membrane potentials comprise a very early step in left-right patterning
    Michael Levin
    Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Cell 111:77-89. 2002
    ..Thus, LR asymmetry determination depends on a very early differential ion flux created by H+/K+-ATPase activity...
  74. ncbi Intrinsic membrane properties of vertebrate vestibular neurons: function, development and plasticity
    H Straka
    L N R S, CNRS UMR 7060 Université René Descartes Paris 5, Paris, France
    Prog Neurobiol 76:349-92. 2005
    ....
  75. pmc Heterogeneous expression of repolarizing, voltage-gated K+ currents in adult mouse ventricles
    Sylvain Brunet
    Department of Molecular Biology and Pharmacology, Washington University Medical School, 660 South Euclid Avenue, Box 8103, St Louis, MO 63110 1093, USA
    J Physiol 559:103-20. 2004
    ....
  76. ncbi Cellular mechanisms of the slow (<1 Hz) oscillation in thalamocortical neurons in vitro
    Stuart W Hughes
    School of Biosciences, Cardiff University, Museum Avenue, CF10 3US, Cardiff, United Kingdom
    Neuron 33:947-58. 2002
    ..These results suggest an active role for the thalamus in shaping the slow (<1 Hz) sleep rhythm...
  77. pmc Regulation of sodium channel function by bilayer elasticity: the importance of hydrophobic coupling. Effects of Micelle-forming amphiphiles and cholesterol
    Jens A Lundbaek
    Novo Nordisk A S, Måløv, Denmark
    J Gen Physiol 123:599-621. 2004
    ..These results provide strong support for the notion that bilayer-protein hydrophobic coupling allows the bilayer elastic properties to regulate membrane protein function...
  78. pmc Biophysics and structure of the patch and the gigaseal
    Thomas M Suchyna
    State University of New York at Buffalo, Department of Physiology and Biophysics, Buffalo, New York 14214, USA
    Biophys J 97:738-47. 2009
    ..Voltage-dependent creep seems to arise from electroosmosis in the seal. Neutralization of negative charges on the seal membrane with low pH decreased the creep rate and reversed the direction of creep at positive pipette potentials...
  79. ncbi Channel noise is essential for perithreshold oscillations in entorhinal stellate neurons
    Alan D Dorval
    Department of Biomedical Engineering, Center for BioDynamics, Boston University, Boston, Massachusetts 02215, USA
    J Neurosci 25:10025-8. 2005
    ..These results provide the first direct demonstration that physiological levels of channel noise can produce qualitative changes in the integrative properties of neurons...
  80. pmc An intersubunit interaction between S4-S5 linker and S6 is responsible for the slow off-gating component in Shaker K+ channels
    Zarah Batulan
    Département de Physique and Groupe d Etude des Protéines Membranaires, Universite de Montreal, Montreal, Quebec H3C 3J7 Canada
    J Biol Chem 285:14005-19. 2010
    ..Development of this interaction occurs under physiological ion conduction and is correlated with pore opening. We, thus, suggest that the above residues stabilize the channel in the open state...
  81. pmc M3 muscarinic acetylcholine receptor expression confers differential cholinergic modulation to neurochemically distinct hippocampal basket cell subtypes
    Christian A Cea-Del Rio
    Program in Developmental Neurobiology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, Bethesda, Maryland 20892, USA
    J Neurosci 30:6011-24. 2010
    ....
  82. ncbi LRRC26 auxiliary protein allows BK channel activation at resting voltage without calcium
    Jiusheng Yan
    Section of Neurobiology, Center for Learning and Memory, University of Texas, Austin, Texas 78712, USA
    Nature 466:513-6. 2010
    ....
  83. doi Large-scale calcium waves traveling through astrocytic networks in vivo
    Nahoko Kuga
    Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo 113 0033, Japan
    J Neurosci 31:2607-14. 2011
    ..This novel phenomenon was heretofore overlooked, probably because of the high vulnerability of astrocytes to light damage; glissandi occurred only when observed at much lower laser intensities than previously used...
  84. ncbi Neuronal integration of synaptic input in the fluctuation-driven regime
    Alexandre Kuhn
    Neurobiology and Biophysics, Institute of Biology III, Albert Ludwigs University, D 79104 Freiburg, Germany
    J Neurosci 24:2345-56. 2004
    ..These findings do not depend on details of the model and, hence, are relevant to cells of other cortical areas as well...
  85. pmc Neocortex network activation and deactivation states controlled by the thalamus
    Akio Hirata
    Department of Neurobiology and Anatomy, Drexel University College of Medicine, 2900 Queen Lane, Philadelphia, PA 19129, USA
    J Neurophysiol 103:1147-57. 2010
    ....
  86. pmc ATP-dependent infra-slow (<0.1 Hz) oscillations in thalamic networks
    Magor L Lorincz
    School of Biosciences, Cardiff University, Cardiff, UK
    PLoS ONE 4:e4447. 2009
    ..We argue that this ISO has a likely non-neuronal origin and may contribute to shaping ISOs in the intact brain...
  87. ncbi Crystal structure of the sodium-potassium pump
    J Preben Morth
    Centre for Membrane Pumps in Cells and Disease PUMPKIN, Danish National Research Foundation, University of Aarhus, Gustav Wieds Vej 10C, DK 8000 Aarhus C, Denmark
    Nature 450:1043-9. 2007
    ..The carboxy terminus of the alpha-subunit is contained within a pocket between transmembrane helices and seems to be a novel regulatory element controlling sodium affinity, possibly influenced by the membrane potential...
  88. doi Evaluation of stochastic differential equation approximation of ion channel gating models
    Ian C Bruce
    Department of Electrical and Computer Engineering, Room ITB A213, McMaster University, 1280 Main Street West, Hamilton, ON L8S 4K1, Canada
    Ann Biomed Eng 37:824-38. 2009
    ....
  89. pmc Voltage is a partial activator of rat thermosensitive TRP channels
    JOSE A MATTA
    Department of Pharmacology, Georgetown University, MedDent SW401, 3900 Reservoir Rd Washington, DC 20007, USA
    J Physiol 585:469-82. 2007
    ..Thus, voltage acts separately but in concert with other stimuli to regulate channel activation, and, therefore, a voltage-sensitive mechanism is unlikely to represent a final, gating mechanism for these channels...
  90. ncbi SK channels gate information processing in vivo by regulating an intrinsic bursting mechanism seen in vitro
    Natalia Toporikova
    Department of Physiology, Center for Nonlinear Dynamics, McGill University, Montreal, Quebec, Canada
    J Neurophysiol 102:2273-87. 2009
    ..Our results show that ion channels located in dendrites can have a profound influence on the processing of sensory input by neurons in vivo through the modulation of an intrinsic bursting mechanism...
  91. pmc Conformational changes and slow dynamics through microsecond polarized atomistic molecular simulation of an integral Kv1.2 ion channel
    Pär Bjelkmar
    Center for Biomembrane Research and Stockholm Bioinformatics Center, Department of Biochemistry and Biophysics, Stockholm University, Stockholm, Sweden
    PLoS Comput Biol 5:e1000289. 2009
    ..Together with lipids binding in matching positions and significant thinning of the membrane also observed in experiments, this provides additional support for the predictive power of microsecond-scale membrane protein simulations...
  92. pmc Alternative splicing in the voltage-gated sodium channel DmNav regulates activation, inactivation, and persistent current
    Wei Hsiang Lin
    Faculty of Life Sciences, University of Manchester, Oxford Road, Manchester M13 9PL, UK
    J Neurophysiol 102:1994-2006. 2009
    ..e., phosphorylation, binding of cofactors, etc.). Our results provide a functional basis for understanding how alternative splicing of a voltage-gated Na+ channel results in diversity in neuronal signaling...
  93. pmc Vesicle pool size at the salamander cone ribbon synapse
    Theodore M Bartoletti
    Department of Ophthalmology and Visual Sciences, University of Nebraska Medical Center, 4050 Durham Research Center, Omaha, NE 68198 5840, USA
    J Neurophysiol 103:419-23. 2010
    ..EPSCs evoked by lengthy depolarization suggest a reserve pool of approximately 90 vesicles per ribbon, similar to the number of additional docking sites further up the ribbon...
  94. ncbi How noise contributes to contrast invariance of orientation tuning in cat visual cortex
    D Hansel
    Laboratoire de Neurophysique et Physiologie du Système Moteur EP 1848 Centre National de la Recherche Scientifique, Universite Rene Descartes, 75270 Paris Cedex 06, France
    J Neurosci 22:5118-28. 2002
    ..This prediction does not depend on the specifics of the model and can be tested experimentally...
  95. pmc Free tubulin modulates mitochondrial membrane potential in cancer cells
    Eduardo N Maldonado
    Center for Cell Death, Injury and Regeneration, Department of Pharmaceutical and Biomedical Sciences, Medical University of South Carolina, Charleston, South Carolina, USA
    Cancer Res 70:10192-201. 2010
    ..Plasma membrane potential assessed by DiBAC(4)(3) was not altered by any of the treatments. We propose that inhibition of VDAC by free tubulin limits mitochondrial metabolism in cancer cells...
  96. pmc Temperature-induced opening of TRPV1 ion channel is stabilized by the pore domain
    JORG GRANDL
    Department of Cell Biology, Scripps Research Institute, La Jolla, California, USA
    Nat Neurosci 13:708-14. 2010
    ..We propose that sequential occupancy of short and long open states on activation provides a mechanism for enhancing temperature sensitivity. Our results suggest that the outer pore is important for the heat sensitivity of thermoTRPs...
  97. pmc Integration and propagation of somatosensory responses in the corticostriatal pathway: an intracellular study in vivo
    Morgane Pidoux
    Centre de Recherche de l Institut du Cerveau et de la Moelle Epiniere, UPMC INSERM UMR S 975, CNRS UMR 7225, Hopital Pitie Salpetriere, Paris, France
    J Physiol 589:263-81. 2011
    ....
  98. ncbi Turning astrocytes from the rostral migratory stream into neurons: a role for the olfactory sensory organ
    Mariana Alonso
    Laboratory for Perception and Memory, Centre National de la Recherche Scientifique, Unité de Recherche Associée 2182, Institut Pasteur, 75724 Paris Cedex 15, France
    J Neurosci 28:11089-102. 2008
    ..New neurons in the adult OB can therefore arise from different neurogenic areas that can be separately regulated...
  99. pmc The plasma membrane calcium ATPase (PMCA) of neurones is electroneutral and exchanges 2 H+ for each Ca2+ or Ba2+ ion extruded
    Roger C Thomas
    Physiological Laboratory, University of Cambridge, Downing Site, Cambridge, UK
    J Physiol 587:315-27. 2009
    ....
  100. pmc Contribution of the Kv3.1 potassium channel to high-frequency firing in mouse auditory neurones
    L Y Wang
    Departments of Pharmacology, Cellular and Molecular Physiology, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06510, USA
    J Physiol 509:183-94. 1998
    ..8. We conclude that in mouse MNTB neurones the Kv3.1 channel contributes to the ability of these cells to lock their firing to high-frequency inputs...
  101. doi Openers of calcium-activated potassium channels and endothelium-dependent hyperpolarizations in the guinea pig carotid artery
    V Leuranguer
    Département Angiologie, Institut de Recherches Servier, 11 rue des Moulineaux, 92150 Suresnes, France
    Naunyn Schmiedebergs Arch Pharmacol 377:101-9. 2008
    ..By contrast, NS-309 is a reasonably potent and selective opener of both SK(Ca) and IK(Ca), and this compound mimics the endothelium-dependent hyperpolarizations to acetylcholine...

Research Grants76

  1. DYNAMICS OF MEMBRANE ORGANIZATION AT NODE OF RANVIER
    Mark Ellisman; Fiscal Year: 1993
    ..interference contrast ((DIC) time-lapse recording in conjunction with optical methods for recording membrane potentials and Ca transients...
  2. DYNAMICS OF MEMBRANE ORGANIZATION AT NODE OF RANVIER
    Mark Ellisman; Fiscal Year: 1992
    ..interference contrast ((DIC) time-lapse recording in conjunction with optical methods for recording membrane potentials and Ca transients...
  3. DYNAMICS OF MEMBRANE ORGANIZATION AT NODE OF RANVIER
    Mark Ellisman; Fiscal Year: 1991
    ..interference contrast ((DIC) time-lapse recording in conjunction with optical methods for recording membrane potentials and Ca transients...
  4. EFFECT OF OXIDANT STRESS ON ALVEOLAR MACROPHAGE FUNCTION
    HENRY FORMAN; Fiscal Year: 1992
    ..We will examine the effects of 2-enals and ROOH upon plasma and mitochondrial membrane potentials, intracellular Ca2+ movements, inositol phosphate turnover, and protein phosphorylation...
  5. EFFECT OF OXIDANT STRESS ON ALVEOLAR MACROPHAGE FUNCTION
    HENRY FORMAN; Fiscal Year: 1991
    ..We will examine the effects of 2-enals and ROOH upon plasma and mitochondrial membrane potentials, intracellular Ca2+ movements, inositol phosphate turnover, and protein phosphorylation...
  6. CHEMOTRANSDUCTION IN THE CAROTID BODY
    Robert Fitzgerald; Fiscal Year: 1999
    ..C) The third goal is to determine the impact of cholinergic agonists and antagonists on membrane potentials and currents in cultured cells and their impact on intracellular calcium...
  7. CHEMOTRANSDUCTION IN THE CAROTID BODY
    Robert Fitzgerald; Fiscal Year: 2000
    ..C) The third goal is to determine the impact of cholinergic agonists and antagonists on membrane potentials and currents in cultured cells and their impact on intracellular calcium...
  8. CHEMOTRANSDUCTION IN THE CAROTID BODY
    Robert Fitzgerald; Fiscal Year: 2001
    ..C) The third goal is to determine the impact of cholinergic agonists and antagonists on membrane potentials and currents in cultured cells and their impact on intracellular calcium...
  9. EXOCYTIC INSERTION OF POTASSIUM CHANNELS IN MEMBRANES
    William Moran; Fiscal Year: 2001
    ..These studies will provide important information about regulation of the pump-leak model that generates membrane potentials and controls cell volume in nutrient- stimulated Na-absorbing epithelia.
  10. PROCESSING AND DEGRADATION OF PROTEOLIPID PROTEIN
    Robert P Skoff; Fiscal Year: 2012
    ..Following PLP's insertion into mitochondria, ATP and mitochondrial membrane potentials dramatically decrease...
  11. ELECTROPHYSIOLOGICAL STUDIES OF VOLTAGE GATED CHANNELS
    Francisco Bezanilla; Fiscal Year: 2000
    ..Distance measurements will be performed at different membrane potentials to assess possible distance during activation of the conductance...
  12. ELECTROPHYSIOLOGICAL STUDIES OF VOLTAGE GATED CHANNELS
    Francisco Bezanilla; Fiscal Year: 2001
    ..Distance measurements will be performed at different membrane potentials to assess possible distance during activation of the conductance...
  13. ELECTROPHYSIOLOGICAL STUDIES OF VOLTAGE GATED CHANNELS
    Francisco Bezanilla; Fiscal Year: 2006
    ..Distance measurements will be performed at different membrane potentials to assess possible distance during activation of the conductance...
  14. ELECTROPHYSIOLOGICAL STUDIES OF VOLTAGE GATED CHANNELS
    Francisco Bezanilla; Fiscal Year: 2002
    ..Distance measurements will be performed at different membrane potentials to assess possible distance during activation of the conductance...
  15. ELECTROPHYSIOLOGICAL STUDIES OF VOLTAGE GATED CHANNELS
    Francisco Bezanilla; Fiscal Year: 2003
    ..Distance measurements will be performed at different membrane potentials to assess possible distance during activation of the conductance...
  16. ELECTROPHYSIOLOGICAL STUDIES OF VOLTAGE GATED CHANNELS
    Francisco Bezanilla; Fiscal Year: 1999
    ..Distance measurements will be performed at different membrane potentials to assess possible distance during activation of the conductance...
  17. ELECTROPHYSIOLOGICAL STUDIES OF VOLTAGE GATED CHANNELS
    Francisco Bezanilla; Fiscal Year: 2007
    ..Distance measurements will be performed at different membrane potentials to assess possible distance during activation of the conductance...
  18. MECHANOELECTRICAL TRANSDUCTION IN ADULT OUTER HAIR CELLS
    David He; Fiscal Year: 2004
    ..Specifically, the transducer currents and membrane potentials as a function of basilar membrane motion wilt be measured...
  19. MECHANOELECTRICAL TRANSDUCTION IN ADULT OUTER HAIR CELLS
    David He; Fiscal Year: 2003
    ..Specifically, the transducer currents and membrane potentials as a function of basilar membrane motion wilt be measured...
  20. Amphetamine Acts as a Molecular Stent in the Dopamine Transporter
    Louis J De Felice; Fiscal Year: 2010
    ..An application of high positive membrane potentials only partially removed AMPH from the transporter, implying AMPH binds tightly and the stent persists long ..
  21. NEURAL CONTROL OF MUSCLE ACTIVITY
    Eberhard Fetz; Fiscal Year: 1999
    ..spikes recorded simultaneously from neighboring neurons, we will compile spike-triggered averages of membrane potentials. This will document unitary post-synaptic potentials (PSPs) produced between cells in different cortical ..
  22. NEURAL CONTROL OF MUSCLE ACTIVITY
    Eberhard Fetz; Fiscal Year: 1999
    ..spikes recorded simultaneously from neighboring neurons, we will compile spike-triggered averages of membrane potentials. This will document unitary post-synaptic potentials (PSPs) produced between cells in different cortical ..
  23. NEURAL CONTROL OF MUSCLE ACTIVITY
    Eberhard Fetz; Fiscal Year: 2000
    ..spikes recorded simultaneously from neighboring neurons, we will compile spike-triggered averages of membrane potentials. This will document unitary post-synaptic potentials (PSPs) produced between cells in different cortical ..
  24. Amphetamine Acts as a Molecular Stent in the Dopamine Transporter
    LOUIS DE FELICE; Fiscal Year: 2009
    ..An application of high positive membrane potentials only partially removed AMPH from the transporter, implying AMPH binds tightly and the stent persists long ..
  25. Insecticide Action at the Local Anesthetic Receptor
    DAVID SODERLUND; Fiscal Year: 2009
    ..PTIs also selectively block mammalian sodium channels at membrane potentials that promote slow sodium channel inactivation...
  26. Insecticide Action at the Local Anesthetic Receptor
    David M Soderlund; Fiscal Year: 2011
    ..PTIs also selectively block mammalian sodium channels at membrane potentials that promote slow sodium channel inactivation...
  27. Insecticide Action at the Local Anesthetic Receptor
    David M Soderlund; Fiscal Year: 2010
    ..PTIs also selectively block mammalian sodium channels at membrane potentials that promote slow sodium channel inactivation...
  28. Insecticide Action at the Local Anesthetic Receptor
    DAVID SODERLUND; Fiscal Year: 2007
    ..PTIs also selectively block mammalian sodium channels at membrane potentials that promote slow sodium channel inactivation...
  29. Search for the Molecular Targets of Phenols
    CYNTHIA SELASSIE; Fiscal Year: 2006
    ..Specific Aim 3 will determine the direct effects of phenols on caspases 1, 3, 8, and 9 and on mitochondrial membrane potentials in vitro, in order to delineate which apoptotic pathway (intrinsic or extrinsic) is compromised in phenol ..
  30. FLOW AND METABOLIC TRACER KINETICS IN HEART
    Robert Marshall; Fiscal Year: 2001
    ..Rhodamine-123 and 99mTc-sestamibi accumulate in active mitochondria that have hemostatic membrane potentials. In all of proposed investigations, the applicants proposed to compare the extraction and retention of the ..
  31. FLOW AND METABOLIC TRACER KINETICS IN HEART
    Robert Marshall; Fiscal Year: 2002
    ..Rhodamine-123 and 99mTc-sestamibi accumulate in active mitochondria that have hemostatic membrane potentials. In all of proposed investigations, the applicants proposed to compare the extraction and retention of the ..
  32. FLOW AND METABOLIC TRACER KINETICS IN HEART
    Robert Marshall; Fiscal Year: 1999
    ..Rhodamine-123 and 99mTc-sestamibi accumulate in active mitochondria that have hemostatic membrane potentials. In all of proposed investigations, the applicants proposed to compare the extraction and retention of the ..
  33. FLOW AND METABOLIC TRACER KINETICS IN HEART
    Robert Marshall; Fiscal Year: 2000
    ..Rhodamine-123 and 99mTc-sestamibi accumulate in active mitochondria that have hemostatic membrane potentials. In all of proposed investigations, the applicants proposed to compare the extraction and retention of the ..
  34. ANESTHETIC ACTIONS ON SINGLE HUMAN BRAIN SODIUM CHANNELS
    DANIEL DUCH; Fiscal Year: 1990
    ..be needed that describe anesthetic effects on the kinetics of sodium channels as they respond to transient membrane potentials. The present project will prepare the ground for such work and the future characterization of other human ..
  35. PHARMACOLOGY OF DEVELOPING CARDIAC TISSUES
    Achilles Pappano; Fiscal Year: 1991
    ..and muscarinic receptor (mAChR) inhibition of biochemical (adenylate cyclase activity), electrical (membrane potentials, ionic currents), and mechanical (inotropic effects) properties of cardiac tissues...
  36. ION TRANSPORT IN SMALL INTESTINE
    William Armstrong; Fiscal Year: 1991
    ..Double barreled (open tip and ion-selective) microelectrodes are used to measure cell membrane potentials and intracellular ionic activities in intact tissue sheets...
  37. PHOTOENERGY TRANSDUCTION IN HALOBACTERIUM
    Walther Stoeckenius; Fiscal Year: 1980
    ..Techniques employed include optical spectroscopy of the pigment and optical detection of pH and membrane potentials using indicating dyes, radioactive tracers for the measuring distribution of ions, and general biochemical ..
  38. Sodium channels and electrogenesis in sensory neurons
    THEODORE CUMMINS; Fiscal Year: 2007
    ..Subthreshold sodium currents, currents that are active at membrane potentials negative to the threshold for action potential generation, can play crucial roles in regulating ..
  39. Sodium channels and electrogenesis in sensory neurons
    THEODORE CUMMINS; Fiscal Year: 2006
    ..Subthreshold sodium currents, currents that are active at membrane potentials negative to the threshold for action potential generation, can play crucial roles in regulating ..
  40. Sodium channels and electrogenesis in sensory neurons
    THEODORE CUMMINS; Fiscal Year: 2009
    ..Subthreshold sodium currents, currents that are active at membrane potentials negative to the threshold for action potential generation, can play crucial roles in regulating ..
  41. Sodium channels and electrogenesis in sensory neurons
    Theodore R Cummins; Fiscal Year: 2010
    ..Subthreshold sodium currents, currents that are active at membrane potentials negative to the threshold for action potential generation, can play crucial roles in regulating ..
  42. Novel ion channel approaches to reentrant arrythymias
    Michael R Rosen; Fiscal Year: 2010
    ..2: Increasing diastolic K conductance should restore depolarized membrane potentials towards normal and enhance excitability for normal myocytes at high stimulation frequencies...
  43. REGULATORY ROLE OF CA++ & MG++ IN VASCULAR SMOOTH MUSCLE
    V Krishnamurty; Fiscal Year: 1980
    ..in vascular smooth muscle Ca ions and Mg ions movements will be studied by measuring cellular ion fluxes, membrane potentials and total Ca ions and Mg ions content of the muscle by atomic absorption spectrophotometry...
  44. Novel ion channel approaches to reentrant arrythymias
    Michael Rosen; Fiscal Year: 2009
    ..2: Increasing diastolic K conductance should restore depolarized membrane potentials towards normal and enhance excitability for normal myocytes at high stimulation frequencies...
  45. BIOCHEMISTRY OF EXCITABLE MEMBRANES
    BRUCE KRUEGER; Fiscal Year: 1980
    ..and other excitable membrane components are controlled by the membrane potential, the method of monitoring membrane potentials in suspensions of particles using fluorescent probes will be refined for synaptosomes and liposomes in ..
  46. CAROTID BODY CHEMOTRANSDUCTION& CARDIOPULMONARY CONTROL
    Machiko Shirahata; Fiscal Year: 1992
    ..freshly dissociated or short-term cultured carotid body Type I cells in which specific ion conductances and membrane potentials are recorded using the patch clamp technique...
  47. SYNAPTIC SOURCES OF HIPPOCAMPAL THETA RHYTHM
    Steven Fox; Fiscal Year: 1991
    ..theta rhythm occurs naturally as the rat moves from place to place and it represents oscillations of the membrane potentials of these same pyramidal cells, so it may be involved in this extraction process...
  48. VASCULAR MUSCLE CALCIUM CURRENTS IN HYPERTENSION
    NANCY RUSCH; Fiscal Year: 1990
    ..of vascular muscle contraction, the predominance of the sustained calcium current occurs at more positive membrane potentials. Since the sustained current likely supplies extracellular calcium current in the SHR may help to explain ..
  49. DIGITALIS EFFECTS ON CARDIAC AUTOMATICITY MECHANISMS
    Mario Vassalle; Fiscal Year: 1980
    ..The microelectrode technique will be used to record membrane potentials and control voltage and a force transducer to measure the force of contraction...
  50. BLOOD PLATELET INTERACTIONS WITH AGGREGANTS
    ELIZABETH SIMONS; Fiscal Year: 1980
    ..changes in intraplatelet concentrations of Ca ion and H ion), spectrofluorimetry (to measure platelet membrane potentials, membrane fluidities, intraplatelet pH), turbimetry (to measure collagen fibril formation), chemical ..
  51. IONIC TRANSFER MECHANISMS IN AIRWAY EPITHELIUM
    William Armstrong; Fiscal Year: 1980
    ..the measurement of transepithelial potential difference, short circuit current, and epithelial cell membrane potentials; determination (by atomic absorption spectrophotometry) of intracellular sodium and potassium ..
  52. Transmitter release from peripheral sensory neurons
    Amy B Macdermott; Fiscal Year: 2010
    ..The first condition involves the interaction between temperature and voltage. At resting membrane potentials, TRPV1 shows measurable, tonic activation at 37oC...
  53. Transmitter release from peripheral sensory neurons
    AMY MACDERMOTT; Fiscal Year: 2009
    ..The first condition involves the interaction between temperature and voltage. At resting membrane potentials, TRPV1 shows measurable, tonic activation at 37oC...
  54. Transmitter release from peripheral sensory neurons
    AMY MACDERMOTT; Fiscal Year: 2006
    ..The first condition involves the interaction between temperature and voltage. At resting membrane potentials, TRPV1 shows measurable, tonic activation at 37oC...
  55. Transmitter release from peripheral sensory neurons
    AMY MACDERMOTT; Fiscal Year: 2007
    ..The first condition involves the interaction between temperature and voltage. At resting membrane potentials, TRPV1 shows measurable, tonic activation at 37oC...
  56. Engineering Microbial Rhodopsins as Optical Voltage Sensors
    ADAM EZRA COHEN; Fiscal Year: 2011
    ..Such a molecule could also be used to probe membrane potentials in mitochondria, cardiac cells, bacteria, or in other non-neuronal cells, and thus would provide a new ..
  57. Cardiomyoplasty in vitro: Host /Doner Cell Interactions
    Nenad Bursac; Fiscal Year: 2006
    ..e. the micropatterned cardiomyocyte strands with inserts made of donor cells. The propagation of cell membrane potentials and intracellular calcium transients in donor cells will be optically mapped in the presence of various ..
  58. Cellular Cardiomyoplasty in Vitro: Host-Donor Cell Interactions
    Nenad Bursac; Fiscal Year: 2007
    ..e. the micropatterned cardiomyocyte strands with inserts made of donor cells. The propagation of cell membrane potentials and intracellular calcium transients in donor cells will be optically mapped in the presence of various ..
  59. The Effects of Resveratrol on Presbyacusis
    Michael Seidman; Fiscal Year: 2003
    ..The differences in mitochondrial function will be studied by measuring the mitochondrial membrane potentials, mitochondrial respiratory enzyme level (complex 1) and mtDNA deletions...
  60. MOLECULAR MECHANISMS OF MEMBRANE TRANSPORT
    David Cafiso; Fiscal Year: 2002
    ..critical process for normal cell metabolism, including the maintenance of ion-gradients, osmotic balance, membrane potentials, and apoptosis...
  61. MOLECULAR MECHANISMS OF MEMBRANE TRANSPORT
    David Cafiso; Fiscal Year: 2000
    ..critical process for normal cell metabolism, including the maintenance of ion-gradients, osmotic balance, membrane potentials, and apoptosis...
  62. MOLECULAR MECHANISMS OF MEMBRANE TRANSPORT
    David Cafiso; Fiscal Year: 2001
    ..critical process for normal cell metabolism, including the maintenance of ion-gradients, osmotic balance, membrane potentials, and apoptosis...