Top Publications

  1. ncbi Verification and validation of diagnostic laboratory tests in clinical virology
    Holger F Rabenau
    Institute for Medical Virology, Johann Wolfgang Goethe University Frankfurt Main, Germany
    J Clin Virol 40:93-8. 2007
  2. pmc Application of next-generation sequencing technologies in virology
    Alan D Radford
    University of Liverpool, Institute of Infection and Global Health, Leahurst Campus, Chester High Road, Neston, South Wirral CH64 7TE, UK
    J Gen Virol 93:1853-68. 2012
  3. ncbi Next-generation sequencing technology in clinical virology
    M R Capobianchi
    National Institute for Infectious Diseases L Spallanzani, Rome, Italy
    Clin Microbiol Infect 19:15-22. 2013
  4. ncbi Laboratory efforts to cultivate noroviruses
    Erwin Duizer
    National Institutes for Public Health and the Environment, PO Box 1, 3720 BA Bilthoven, The Netherlands
    J Gen Virol 85:79-87. 2004
  5. pmc Comparison of two commercially available dengue virus (DENV) NS1 capture enzyme-linked immunosorbent assays using a single clinical sample for diagnosis of acute DENV infection
    Kovi Bessoff
    Centers for Disease Control and Prevention, Division of Vector Borne Infectious Diseases, Dengue Branch, SanJuan, Puerto Rico 00920
    Clin Vaccine Immunol 15:1513-8. 2008
  6. ncbi Accuracy of rapid influenza diagnostic tests: a meta-analysis
    Caroline Chartrand
    CHU Sainte Justine, Universite de Montreal, Montreal Chest Institute, Quebec, Canada
    Ann Intern Med 156:500-11. 2012
  7. pmc Identification of respiratory viruses in adults: nasopharyngeal versus oropharyngeal sampling
    David Lieberman
    Pulmonary Unit, Soroka Medical Center, Beer Sheva 84101, Israel
    J Clin Microbiol 47:3439-43. 2009
  8. ncbi Improved LNA probe-based assay for the detection of African and South American yellow fever virus strains
    Manfred Weidmann
    University Medical Center, Department of Virology, Kreuzbergring 57, 37075 Gottingen, Germany
    J Clin Virol 48:187-92. 2010
  9. pmc Application of broad-spectrum resequencing microarray for genotyping rhabdoviruses
    Laurent Dacheux
    Institut Pasteur, Lyssavirus Dynamics and Host Adaptation Unit, Paris, France
    J Virol 84:9557-74. 2010
  10. ncbi Detection of Cereal yellow dwarf virus using small interfering RNAs and enhanced infection rate with Cocksfoot streak virus in wild cocksfoot grass (Dactylis glomerata)
    Denise W Pallett
    NERC Centre for Ecology and Hydrology, Oxford, Oxford OX1 3SR, UK
    J Virol Methods 168:223-7. 2010

Detail Information

Publications320 found, 100 shown here

  1. ncbi Verification and validation of diagnostic laboratory tests in clinical virology
    Holger F Rabenau
    Institute for Medical Virology, Johann Wolfgang Goethe University Frankfurt Main, Germany
    J Clin Virol 40:93-8. 2007
    ..procedures and describes minimum requirements for verification and validation of diagnostic assays in clinical virology including instructions for CE/IVD-labeled as well as for self-developed ("home-brewed") tests or test systems...
  2. pmc Application of next-generation sequencing technologies in virology
    Alan D Radford
    University of Liverpool, Institute of Infection and Global Health, Leahurst Campus, Chester High Road, Neston, South Wirral CH64 7TE, UK
    J Gen Virol 93:1853-68. 2012
    ..In addition, improved methods for analysis will become available, opening yet further applications in virology including routine diagnostic work on individuals, and new understanding of the interaction between viral and host ..
  3. ncbi Next-generation sequencing technology in clinical virology
    M R Capobianchi
    National Institute for Infectious Diseases L Spallanzani, Rome, Italy
    Clin Microbiol Infect 19:15-22. 2013
    ..The diagnostic application of NGS is just around the corner...
  4. ncbi Laboratory efforts to cultivate noroviruses
    Erwin Duizer
    National Institutes for Public Health and the Environment, PO Box 1, 3720 BA Bilthoven, The Netherlands
    J Gen Virol 85:79-87. 2004
    ..Thus, attempts to develop a method for the cultivation of NoV were unsuccessful...
  5. pmc Comparison of two commercially available dengue virus (DENV) NS1 capture enzyme-linked immunosorbent assays using a single clinical sample for diagnosis of acute DENV infection
    Kovi Bessoff
    Centers for Disease Control and Prevention, Division of Vector Borne Infectious Diseases, Dengue Branch, SanJuan, Puerto Rico 00920
    Clin Vaccine Immunol 15:1513-8. 2008
    ..We concluded that the NS1 capture ELISA is a useful tool that can improve testing algorithms to diagnose DENV infection in single samples from acute and early convalescent cases...
  6. ncbi Accuracy of rapid influenza diagnostic tests: a meta-analysis
    Caroline Chartrand
    CHU Sainte Justine, Universite de Montreal, Montreal Chest Institute, Quebec, Canada
    Ann Intern Med 156:500-11. 2012
    ..Timely diagnosis of influenza can help clinical management...
  7. pmc Identification of respiratory viruses in adults: nasopharyngeal versus oropharyngeal sampling
    David Lieberman
    Pulmonary Unit, Soroka Medical Center, Beer Sheva 84101, Israel
    J Clin Microbiol 47:3439-43. 2009
    ..Sampling by all three methods is required for the maximal detection of respiratory viruses...
  8. ncbi Improved LNA probe-based assay for the detection of African and South American yellow fever virus strains
    Manfred Weidmann
    University Medical Center, Department of Virology, Kreuzbergring 57, 37075 Gottingen, Germany
    J Clin Virol 48:187-92. 2010
    ..Real-time assays for Yellow fever virus (YFV) would help to improve acute diagnostics in outbreak investigations...
  9. pmc Application of broad-spectrum resequencing microarray for genotyping rhabdoviruses
    Laurent Dacheux
    Institut Pasteur, Lyssavirus Dynamics and Host Adaptation Unit, Paris, France
    J Virol 84:9557-74. 2010
  10. ncbi Detection of Cereal yellow dwarf virus using small interfering RNAs and enhanced infection rate with Cocksfoot streak virus in wild cocksfoot grass (Dactylis glomerata)
    Denise W Pallett
    NERC Centre for Ecology and Hydrology, Oxford, Oxford OX1 3SR, UK
    J Virol Methods 168:223-7. 2010
    ..Opportunities are discussed for using siRNA sequencing approaches in virus survey and other ecology studies under field conditions...
  11. pmc Comparison of three multiplex PCR assays for the detection of respiratory viral infections: evaluation of xTAG respiratory virus panel fast assay, RespiFinder 19 assay and RespiFinder SMART 22 assay
    Mareike Dabisch-Ruthe
    Institut fur Laboratoriums und Transfusionsmedizin, Herz und Diabeteszentrum Nordrhein Westfalen, Universitatsklinik der Ruhr Universitat Bochum, Bad Oeynhausen, Germany
    BMC Infect Dis 12:163. 2012
    ..Therefore, the identification of the causative viruses and bacteria is only feasible using multiplex PCR or several monoplex PCR tests in parallel...
  12. pmc Basic statistical considerations in virological experiments
    Barbra A Richardson
    Harborview Medical Center, University of Washington, Box 359909, 325 Ninth Avenue, Seattle, WA 98104 2499, USA
    J Virol 79:669-76. 2005
  13. ncbi Optimized PCR assay for detection of white spot syndrome virus (WSSV)
    Linda M Nunan
    Department of Veterinary Science and Microbiology, University of Arizona, Tucson, AZ 85721, USA
    J Virol Methods 171:318-21. 2011
    ..Various isolates were tested using the modified procedure, to ensure that the assay was able to detect WSSV from different geographical locations...
  14. pmc Rapid identification viruses from nasal pharyngeal aspirates in acute viral respiratory infections by RT-PCR and electrospray ionization mass spectrometry
    Kuan Fu Chen
    Johns Hopkins University, Department of Medicine, Baltimore, MD 21205, USA
    J Virol Methods 173:60-6. 2011
    ..consented, and "excess" frozen archived nasopharyngeal aspirates were analysed; 46 were positive by conventional virology and 69 by RT-PCR/ESI-MS, among which there were six samples with multiple viral pathogens detected...
  15. pmc Development of phoH as a novel signature gene for assessing marine phage diversity
    Dawn B Goldsmith
    College of Marine Science, University of South Florida, St Petersburg, FL 33701, USA
    Appl Environ Microbiol 77:7730-9. 2011
    ..Each depth and geographic location had a distinct phoH composition, although most phoH clusters were recovered from multiple sites. Overall, phoH is an effective signature gene for examining phage diversity in the marine environment...
  16. ncbi Evaluation of genotypic tropism prediction tests compared with in vitro co-receptor usage in HIV-1 primary isolates of diverse subtypes
    Elena Delgado
    HIV Biology and Variability Department, Centro Nacional de Microbiologia, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain
    J Antimicrob Chemother 67:25-31. 2012
  17. pmc Reanalysis of coreceptor tropism in HIV-1-infected adults using a phenotypic assay with enhanced sensitivity
    Timothy J Wilkin
    Division of Infectious Disease, Weill Cornell Medical College, New York, New York 10011, USA
    Clin Infect Dis 52:925-8. 2011
    ..Nine percent to 26% of patients with CCR5-tropic virus by the original Trofile assay had CXCR4-using virus by TF-ES. Lower CD4 cell counts were associated with CXCR4-using virus in all cohorts...
  18. pmc Simultaneously typing nine serotypes of enteroviruses associated with hand, foot, and mouth disease by a GeXP analyzer-based multiplex reverse transcription-PCR assay
    Xiumei Hu
    State Key Laboratory for Genetic Engineering and Molecular Virology, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, People s Republic of China
    J Clin Microbiol 50:288-93. 2012
    ..59% (25/27). In conclusion, the GeXP assay is a rapid, cost-effective, and high-throughput method for typing nine serotypes of HFMD-associated enteroviruses...
  19. ncbi Detection of pandemic influenza A H1N1 virus by multiplex reverse transcription-PCR with a GeXP analyzer
    Meng Qin
    National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Xuan Wu District, Beijing 100052, China
    J Virol Methods 168:255-8. 2010
    ..This technique has the potential to provide both a powerful method to enhance surveillance of influenza and a platform for investigating the differentiation of other similar pathogens...
  20. pmc Analysis of prototype foamy virus particle-host cell interaction with autofluorescent retroviral particles
    Kristin Stirnnagel
    Institut fur Virologie, Medizinische Fakultät Carl Gustav Carus, Technische Universitat Dresden, Dresden, Germany
    Retrovirology 7:45. 2010
    ..Second, the unusually broad host range of FV is thought to be due to use of a very common entry receptor present on host cell plasma membranes, because all cell lines tested in vitro so far are permissive...
  21. pmc Epidemiology and clinical presentations of the four human coronaviruses 229E, HKU1, NL63, and OC43 detected over 3 years using a novel multiplex real-time PCR method
    E R Gaunt
    Centre for Infectious Diseases, University of Edinburgh, Summerhall, Edinburgh EH9 1QH, United Kingdom
    J Clin Microbiol 48:2940-7. 2010
  22. pmc Microarray-based detection and genotyping of viral pathogens
    David Wang
    Department of Biochemistry and Biophysics, University of California, San Francisco 94143, USA
    Proc Natl Acad Sci U S A 99:15687-92. 2002
    ..This method is versatile and greatly expands the spectrum of detectable viruses in a single assay while simultaneously providing the capability to discriminate among viral subtypes...
  23. pmc Inefficient human immunodeficiency virus replication in mobile lymphocytes
    Marion Sourisseau
    Groupe Virus et Immunité, URA CNRS 1930, Institut Pasteur, 28 rue du Dr Roux, 75724 Paris Cedex 15, France
    J Virol 81:1000-12. 2007
    ..Altogether, these results indicate that cell-to-cell transfer is the predominant mode of HIV spread and help to explain why this virus replicates so efficiently in lymphoid organs...
  24. pmc Development of a virus concentration method and its application to detection of enterovirus and norwalk virus from coastal seawater
    Hiroyuki Katayama
    Department of Urban Engineering, School of Engineering, University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 8656, Japan
    Appl Environ Microbiol 68:1033-9. 2002
    ..Naturally occurring Norwalk viruses from 2 liters of Tokyo Bay water in winter and infectious enteroviruses from 2 liters of recreational coastal seawater in summer were detected by using this viral concentration method...
  25. ncbi Detecting 2009 pandemic influenza A (H1N1) virus infection: availability of diagnostic testing led to rapid pandemic response
    D B Jernigan
    Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
    Clin Infect Dis 52:S36-43. 2011
    ..Future planning efforts should identify ways to improve availability of reliable testing to manage patient care and approaches for optimal use of molecular testing for detecting and controlling emerging influenza virus strains...
  26. pmc Development and characterization of a novel single-cycle recombinant-virus assay to determine human immunodeficiency virus type 1 coreceptor tropism
    Jeannette M Whitcomb
    Monogram Biosciences, Inc, 345 Oyster Point Boulevard, South San Francisco, CA 94080, USA
    Antimicrob Agents Chemother 51:566-75. 2007
  27. pmc The history of tumor virology
    Ronald T Javier
    Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas 77030, USA
    Cancer Res 68:7693-706. 2008
    In the century since its inception, the field of tumor virology has provided groundbreaking insights into the causes of human cancer...
  28. pmc Detection of a novel astrovirus in brain tissue of mink suffering from shaking mink syndrome by use of viral metagenomics
    Anne Lie Blomström
    Department of Biomedical Sciences and Veterinary Public Health, Section of Virology, Swedish University of Agricultural Sciences, Box 7036, S 750 07 Uppsala, Sweden
    J Clin Microbiol 48:4392-6. 2010
  29. pmc Comparison of three commercially available dengue NS1 antigen capture assays for acute diagnosis of dengue in Brazil
    Monique da Rocha Queiroz Lima
    Flavivirus Laboratory, Oswaldo Cruz Institute FIOCRUZ, Rio de Janeiro, Brazil
    PLoS Negl Trop Dis 4:e738. 2010
    ..The flavivirus non-structural protein NS1, a highly conserved and secreted glycoprotein, is a candidate protein for rapid diagnosis of dengue in endemic countries...
  30. ncbi Clinical virology in real time
    Hubert G M Niesters
    Department of Virology, Erasmus MC, University Medical Center Rotterdam, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
    J Clin Virol 25:S3-12. 2002
    The ability to detect nucleic acids has had and still has a major impact on diagnostics in clinical virology. Both quantitative and qualitative techniques, whether signal or target amplification based systems, are currently used routinely ..
  31. ncbi Hepatitis E virus ORF3 antigens derived from genotype 1 and 4 viruses are detected with varying efficiencies by an anti-HEV enzyme immunoassay
    Hongxia Ma
    Department of Cell Biology, National Institute for Control of Pharmaceutical and Biological Products, Beijing, China
    J Med Virol 83:827-32. 2011
    ..The HEV ORF3 polypeptide contains genotype-specific antigens and the antigen-antibody reactions between the same genotypes were stronger than those between different genotypes...
  32. ncbi Detection of Puumala and Rift Valley Fever virus by quantitative RT-PCR and virus viability tests in samples of blood dried and stored on filter paper
    Jonas Näslund
    Department of Clinical Microbiology, Division of Infectious Diseases, Umea University, SE 901 85 Umea, Sweden
    J Virol Methods 178:186-90. 2011
    ..This approach might facilitate sampling and analysis of other RNA viruses from human or animal sources and could be used for field studies in remote areas or in developing countries...
  33. ncbi Viral meningitis
    David R Chadwick
    Department of Infection and Travel Medicine, The James Cook University Hospital, Middlesbrough TS4 3BW, UK
    Br Med Bull 75:1-14. 2005
    ..This review focuses on the presentation, diagnosis and management of viral meningitis and also covers the prevention of meningitis for pathogens where effective vaccines are available...
  34. pmc Near-atomic-resolution cryo-EM for molecular virology
    Corey F Hryc
    National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Curr Opin Virol 1:110-7. 2011
    ..This paper reviews the recent advances in near-atomic-resolution cryo-EM for probing the mechanisms of virus assembly and morphogenesis...
  35. pmc Abundance of multiple high-risk human papillomavirus (HPV) infections found in cervical cells analyzed by use of an ultrasensitive HPV genotyping assay
    Markus Schmitt
    Department of Genome Modifications and Carcinogenesis F020, Research Program Infection and Cancer, German Cancer Research Center DKFZ, Im Neuenheimer Feld 242, Heidelberg 69120, Germany
    J Clin Microbiol 48:143-9. 2010
    ..As a consequence, PCR-TS-MPG appears to be highly suited for analysis of the significance of multiple infections in the development of cervical cancer and for the study the natural history and the latency of HPV...
  36. ncbi Detection and identification of Rift Valley fever virus in mosquito vectors by quantitative real-time PCR
    D Mwaengo
    Department of Medical Microbiology, University of Nairobi, PO Box 19676 00202, KNH, Nairobi, Kenya
    Virus Res 169:137-43. 2012
    ..The data confirms the utility of qRT-PCR for identification and initial quantification of virus in mosquito samples during RVFV outbreaks...
  37. pmc Monoclonal antibody-based serological methods for detection of Cucumber green mottle mosaic virus
    Haili Shang
    State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou, Zhejiang, China
    Virol J 8:228. 2011
  38. ncbi Detection of white spot syndrome virus by polymerase chain reaction performed under insulated isothermal conditions
    Yun Long Tsai
    Department of Research and Development, GeneReach Biotechnology Corporation, Taichung, Taiwan
    J Virol Methods 181:134-7. 2012
    ..iiPCR has a potential as a low-cost method for sensitive, specific and rapid detection of pathogens...
  39. pmc Comparison of the Luminex Respiratory Virus Panel fast assay with in-house real-time PCR for respiratory viral infection diagnosis
    Naomi J Gadsby
    Specialist Virology Centre, Department of Laboratory Medicine, Royal Infirmary of Edinburgh, Little France Crescent, Edinburgh, EH16 4SA, United Kingdom
    J Clin Microbiol 48:2213-6. 2010
    ..and multiplex real-time PCRs in the testing of 286 respiratory specimens submitted to the Edinburgh Specialist Virology Centre for routine diagnosis of viral infection between December 2007 and February 2009...
  40. pmc Sensitivity of influenza rapid diagnostic tests to H5N1 and 2009 pandemic H1N1 viruses
    Yuko Sakai-Tagawa
    Division of Virology, Department of Microbiology and Immunology, University of Tokyo, Shirokanedai, Minato ku, Tokyo 108 8639, Japan
    J Clin Microbiol 48:2872-7. 2010
    ..Our results indicate that the detection sensitivity to swine origin H1N1 viruses varies widely among IRDTs, with some tests lacking sufficient sensitivity to detect the early stages of infection when the virus load is low...
  41. ncbi Metagenomics for the discovery of novel human viruses
    Patrick Tang
    British Columbia Centre for Disease Control, Department of Pathology and Laboratory Medicine, University of British Columbia, 655 West 12th Avenue, Vancouver, BC, V5Z 4R4, Canada
    Future Microbiol 5:177-89. 2010
    ..Sequencing-based methods have also been successfully employed to detect novel viruses and have the potential to detect the full spectrum of viruses, including those present in low numbers...
  42. pmc RNA and DNA bacteriophages as molecular diagnosis controls in clinical virology: a comprehensive study of more than 45,000 routine PCR tests
    Laetitia Ninove
    UMR190, Université de la Méditerranée and Institut de Recherche pour le Développement, Marseille, France
    PLoS ONE 6:e16142. 2011
    ..proved to be cost-effective, flexible and adaptable to various technical procedures of real-time PCR detection in virology. It represents a valuable strategy for enhancing the quality of routine molecular diagnosis in laboratories that ..
  43. ncbi Comparative evaluation of the QIAGEN QIAsymphony® SP system and bioMérieux NucliSens easyMAG automated extraction platforms in a clinical virology laboratory
    Adele V Lee
    Department of Infection HPA Lead Microbiology Laboratory for London, Barts and The London NHS Trust, London, E1 2ES, UK
    J Clin Virol 52:339-43. 2011
    ..The QIAGEN QIAsymphony(®) SP is an automated system that can process a variety of different sample types for nucleic acid extraction...
  44. ncbi Detection by PCR of human papillomavirus in Colombia: Comparison of GP5+/6+ and MY09/11 primer sets
    Milena Camargo
    Molecular Biology Department, Fundacion Instituto de Inmunologia de Colombia, Bogota, Colombia
    J Virol Methods 178:68-74. 2011
    ..This study highlights the importance of suitable assessment of HPV epidemiological profiles; screening programs must also be strengthened to broaden the coverage of the most vulnerable populations...
  45. pmc First international external quality assessment of molecular detection of Crimean-Congo hemorrhagic fever virus
    Camille Escadafal
    Centre for Biosafety, Robert Koch Institute, Berlin, Germany
    PLoS Negl Trop Dis 6:e1706. 2012
    ..These results indicate that there is still a need for improving testing conditions and standardizing protocols for the molecular detection of Crimean-Congo hemorrhagic fever virus...
  46. ncbi Full HPV typing by a single restriction enzyme
    Enrique Santiago
    Departamento de Biologia Funcional, Universidad de Oviedo, 33071 Oviedo, Spain
    J Clin Virol 37:38-46. 2006
    ..However, we think that these methods have not taken full advantage of the high diversity of the known restriction enzymes...
  47. ncbi Development of a convenient immunochromatographic strip for the diagnosis of infection with Japanese encephalitis virus in swine
    Yaoming Li
    State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei 430070, China
    J Virol Methods 168:51-6. 2010
    ..3% and 85.7% respectively as compared to that of RT-PCR. This suggests that the MAbs-based ICS test can be used as a convenient method for the rapid detection of JEV in infected swine samples...
  48. ncbi Description and validation of a novel real-time RT-PCR enterovirus assay
    Weston C Hymas
    Institute for Clinical and Experimental Pathology, Salt Lake City, Utah 84108, USA
    Clin Chem 54:406-13. 2008
    ..The assay includes an RNA internal control and incorporates modified nucleotide chemistry...
  49. ncbi Development of a gene capture method to rescue a large deletion mutant of human cytomegalovirus
    Kalpana Dulal
    Department of Microbiology and Molecular Genetics, UMDNJ New Jersey Medical School, Newark, 07101, USA
    J Virol Methods 157:180-7. 2009
    ..This method will be useful particularly for cloning large DNA fragments from any genome without introducing undesired mutations by traditional PCR-based approaches...
  50. pmc Methods for sampling of airborne viruses
    Daniel Verreault
    Centre de Recherche, Hopital Laval, Institut Universitaire de Cardiologie et de Pneumologie, 2725 Chemin Ste Foy, Quebec City, Quebec, Canada G1V 4G5
    Microbiol Mol Biol Rev 72:413-44. 2008
    ..Lastly, general recommendations are made regarding future studies on the sampling of airborne viruses...
  51. pmc Evaluation of a commercial SD dengue virus NS1 antigen capture enzyme-linked immunosorbent assay kit for early diagnosis of dengue virus infection
    Seok Mui Wang
    Department of Medical Microbiology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia
    J Clin Microbiol 48:2793-7. 2010
    ..97%. Combining this assay with an IgM and/or IgG assay will increase the sensitivity of detection, especially in areas with a higher prevalence of secondary dengue virus infections...
  52. pmc Phages harboring specific peptides that recognize the N protein of the porcine reproductive and respiratory syndrome virus distinguish the virus from other viruses
    Xiaofeng Ren
    College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai St, Xiangfang District, 150030 Harbin, China
    J Clin Microbiol 48:1875-81. 2010
    ..More importantly, the selected phages harboring specific peptides that recognize the N protein of PRRSV were able to efficiently distinguish PRRSV from other viruses in enzyme-linked immunosorbent assays...
  53. pmc Diagnostic assays for active infection with human herpesvirus 6 (HHV-6)
    Mary T Caserta
    Department of Pediatrics, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, United States
    J Clin Virol 48:55-7. 2010
    ..Chromosomal integration of HHV-6 also occurs. Previous studies have suggested that the detection of HHV-6 DNA in plasma is an accurate marker of active viral replication...
  54. pmc Improved PCR methods for detection of African rabies and rabies-related lyssaviruses
    Jessica Coertse
    Department of Microbiology and Plant Pathology, Faculty of Natural and Agricultural Sciences, University of Pretoria, Pretoria 0001, South Africa
    J Clin Microbiol 48:3949-55. 2010
    ..Alternatively, the hnRT-PCR assay can be used in laboratories that do not have access to expensive real-time PCR equipment for sensitive diagnosis of lyssaviruses...
  55. ncbi Immunofluorescence versus xTAG multiplex PCR for the detection of respiratory picornavirus infections in children
    Christina Schindera
    University Children s Hospital, Inselspital, and Institute of Infectious Diseases, University of Bern, Freiburgstrasse 3, 3010 Bern, Switzerland
    J Clin Virol 48:223-5. 2010
    ..Immunofluorescence (IF), widely used to detect other respiratory viruses, has recently been introduced as a promising detection method for respiratory picornaviruses...
  56. ncbi Development of a blocking ELISA for detection of serum neutralizing antibodies against porcine circovirus type 2
    Liping Huang
    Division of Swine Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin, China
    J Virol Methods 171:26-33. 2011
    ..This report demonstrates that the assay is a simple, specific, sensitive and convenient method for epidemiological surveys and evaluations of serum neutralizing antibodies against PCV2...
  57. ncbi Comparison of the performance of direct fluorescent antibody staining, a point-of-care rapid antigen test and virus isolation with that of RT-PCR for the detection of novel 2009 influenza A (H1N1) virus in respiratory specimens
    Tina Ganzenmueller
    Institute of Virology, Hannover Medical School, Carl Neuberg Strasse 1, 30625 Hannover, Germany
    J Med Microbiol 59:713-7. 2010
    ..In summary, DFA staining is superior to POC testing and may be appropriate for patients expected to have a rather high level of virus replication. Nevertheless, in DFA-negative specimens, A/H1N1/2009 should be excluded by RT-PCR...
  58. pmc Comparison of a real-time reverse transcriptase PCR assay and a culture technique for quantitative assessment of viral load in children naturally infected with respiratory syncytial virus
    Stephanie M Perkins
    Unversity of Tennessee School of Medicine, Memphis, TN, USA
    J Clin Microbiol 43:2356-62. 2005
    ..The utility of the RTrtPCR quantification technique for clinical studies would be solidified after its correlation with RSV disease severity is established...
  59. pmc GC content-based pan-pox universal PCR assays for poxvirus detection
    Yu Li
    Centers for Disease Control and Prevention, National Center for Zoonotic, Vector Borne, and Enteric Diseases, Division of Viral and Rickettsial Diseases, Poxvirus and Rabies Branch, Atlanta, Georgia 30329, USA
    J Clin Microbiol 48:268-76. 2010
    ..The pan-pox assays represent an important advance for the screening and diagnosis of human and animal poxvirus infections, and the technology used is accessible to many laboratories worldwide...
  60. pmc Assessment of MagNA pure LC extraction system for detection of human papillomavirus (HPV) DNA in PreservCyt samples by the Roche AMPLICOR and LINEAR ARRAY HPV tests
    Matthew P Stevens
    Department of Microbiology, The Royal Women s Hospital, and Department of Obstetrics and Gynaecology, University of Melbourne, Victoria, Australia
    J Clin Microbiol 44:2428-33. 2006
  61. ncbi Repeated HIV-1 resistance genotyping external quality assessments improve virology laboratory performance
    Diane Descamps
    Laboratoire de Virologie, CHU Bichat Claude Bernard, Paris, France
    J Med Virol 78:153-60. 2006
    The performance of French virology laboratories belonging to the ANRS network has been assessed annually for 3 years. The performance of these laboratories was compared between the years 2002 and 2003...
  62. pmc Retrovirology and young Turks
    Kuan Teh Jeang
    Retrovirology 1:1. 2004
  63. pmc Comparison between pernasal flocked swabs and nasopharyngeal aspirates for detection of common respiratory viruses in samples from children
    Afaf Abu-Diab
    Caritas Baby Hospital, Bethlehem
    J Clin Microbiol 46:2414-7. 2008
    ..5% and 100%, respectively. The excellent sensitivity of the flocked swab samples in combination with the rapid means by which they may be collected makes them an alternative to NPA samples, whose collection is more invasive...
  64. pmc Establishment of fruit bat cells (Rousettus aegyptiacus) as a model system for the investigation of filoviral infection
    Verena Krähling
    Institut fur Virologie, Philipps Universitat Marburg, Marburg, Germany
    PLoS Negl Trop Dis 4:e802. 2010
  65. pmc Improved detection of respiratory viruses in pediatric outpatients with acute respiratory illness by real-time PCR using nasopharyngeal flocked swabs
    Patrick Kiio Munywoki
    KEMRI Wellcome Trust Research Programme, Kilifi, Kenya
    J Clin Microbiol 49:3365-7. 2011
    ..6% versus 79.2%; P = 0.0043). NFS collection was noninferior to NW collection in the detection of RSV by IF...
  66. pmc Comparison of the Luminex xTAG respiratory viral panel with xTAG respiratory viral panel fast for diagnosis of respiratory virus infections
    Kanti Pabbaraju
    Provincial Laboratory for Public Health, Calgary, Alberta, Canada
    J Clin Microbiol 49:1738-44. 2011
    ..A small number of samples positive for influenza A virus, respiratory syncytial virus B, human metapneumovirus, and parainfluenza virus type 1 were not detected by the RVP Classic assay and in general had low viral loads...
  67. ncbi Double-labelled HIV-1 particles for study of virus-cell interaction
    Marko Lampe
    Department of Virology, Universitatsklinikum Heidelberg, Im Neuenheimer Feld 324, 69120 Heidelberg, Germany
    Virology 360:92-104. 2007
    ..We present here a detailed characterization of this novel tool together with exemplary live cell imaging studies, demonstrating its suitability for real-time analyses of HIV-cell interaction...
  68. pmc The design and validation of a novel phenotypic assay to determine HIV-1 coreceptor usage of clinical isolates
    Nina H Lin
    Infectious Diseases Unit, Massachusetts General Hospital, 55 Fruit Street, Jackson 504, Boston, MA 02114, USA
    J Virol Methods 169:39-46. 2010
    ..This assay provides a sensitive, efficient and relatively low-cost approach suitable for use by research laboratories for assessing HIV-1 coreceptor usage of plasma samples...
  69. pmc Long PCR and its application to hepatitis viruses: amplification of hepatitis A, hepatitis B, and hepatitis C virus genomes
    R Tellier
    Hepatitis Viruses Section, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland, USA
    J Clin Microbiol 34:3085-91. 1996
    ..We amplified the nearly full-length sequence of each of these viruses from a small number of viral genomes, demonstrating the sensitivity of the process...
  70. pmc Complementary monoclonal antibody-based dot ELISA for universal detection of H5 avian influenza virus
    Fang He
    Animal Health Biotechnology, Temasek Life Sciences Laboratory, National University of Singapore, Singapore
    BMC Microbiol 10:330. 2010
    ..Rapid diagnosis and surveillance for H5 subtype viruses are critical for the control of H5N1 infection...
  71. ncbi Screening of mosquitoes using SYBR Green I-based real-time RT-PCR with group-specific primers for detection of Flaviviruses and Alphaviruses in Taiwan
    Cheng Fen Yang
    Department of Health, Research and Diagnostic Center, Centers for Disease Control, Taipei, Taiwan
    J Virol Methods 168:147-51. 2010
    ..This study is the first to quantify the viral load in individual mosquitoes over the course of a 16-day extrinsic incubation period...
  72. pmc The discovery of endogenous retroviruses
    Robin A Weiss
    Division of Infection and Immunity, University College London, 46 Cleveland Street, London W1T 4JF, UK
    Retrovirology 3:67. 2006
    ..Here I recount some of the key observations in birds and mammals that led to the discovery of ERV, and comment on their evolution, cross-species dispersion, and what remains to be elucidated...
  73. pmc A highly efficient protocol of generating and analyzing VZV ORF deletion mutants based on a newly developed luciferase VZV BAC system
    Zhen Zhang
    Department of Microbiology and Molecular Genetics, UMDNJ New Jersey Medical School, 225 Warren Street, Newark, NJ 07101 1709, USA
    J Virol Methods 148:197-204. 2008
    ..In this study, a highly efficient protocol has been developed exploiting the new luciferase VZV BAC system to rapidly isolate and characterize VZV ORF deletion mutants by growth curve analysis in cell culture...
  74. ncbi Development of a porcine epidemic diarrhea virus M protein-based ELISA for virus detection
    Xiaofeng Ren
    Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai Street, Xiangfang District, Harbin 150030, China
    Biotechnol Lett 33:215-20. 2011
    ..Other viruses, porcine transmissible gastroenteritis coronavirus, avian infectious bronchitis coronavirus, porcine reproductive and respiratory syndrome virus, classic swine fever virus and porcine pseudorabies virus, were unreactive...
  75. ncbi Rapid identification of neuraminidase inhibitor resistance mutations in seasonal influenza virus A(H1N1), A(H1N1)2009, and A(H3N2) subtypes by melting point analysis
    M Redlberger-Fritz
    Department of Virology, Medical University Vienna, Kinderspitalgasse 15, 1095, Vienna, Austria
    Eur J Clin Microbiol Infect Dis 31:1593-601. 2012
    ..MPA enables a clear identification of mutations associated with antiviral resistance...
  76. ncbi Development of a sensitive real-time reverse transcriptase PCR assay with an internal control to detect and quantify chikungunya virus
    Philippe Laurent
    Services de Bactériologie Parasitologie Virologie et Hygiène, Groupe Hospitalier Sud Reunion, Saint Pierre, La Reunion
    Clin Chem 53:1408-14. 2007
    ..Facing an outbreak of unprecedented magnitude, we developed a rapid, sensitive, and reliable assay for the detection and quantification of CHIKV in plasma samples...
  77. pmc Systems virology: host-directed approaches to viral pathogenesis and drug targeting
    G Lynn Law
    Department of Microbiology and Washington National Primate Research Center, University of Washington, Box 358070, Seattle, Washington 98195, USA
    Nat Rev Microbiol 11:455-66. 2013
    High-throughput molecular profiling and computational biology are changing the face of virology, providing a new appreciation of the importance of the host in viral pathogenesis and offering unprecedented opportunities for better ..
  78. pmc Diagnostic virology: from animals to automation
    G D Hsiung
    Yale J Biol Med 57:727-33. 1984
    ..Discovery of unknown viral agents still requires continued search and diligent effort...
  79. ncbi High concordance of genotypic coreceptor prediction in plasma-viral RNA and proviral DNA of HIV-1 subtype C: implications for use of whole blood DNA in resource-limited settings
    Soham Gupta
    Department of Microbiology, St John s Medical College and Hospital, Bangalore, India
    J Antimicrob Chemother 68:2003-6. 2013
    ..We aimed to evaluate the performance of cell-associated proviral DNA against plasma-derived viral RNA as the genetic material for GTT in an Indian clinical setting...
  80. pmc Evaluation of the Cepheid Xpert Flu Assay for rapid identification and differentiation of influenza A, influenza A 2009 H1N1, and influenza B viruses
    S M Novak-Weekley
    Southern California Permanente Medical Group, North Hollywood, California, USA
    J Clin Microbiol 50:1704-10. 2012
    ..5%, 100%, and 93.8%, respectively. We conclude that the Cepheid Xpert Flu Assay is an accurate and rapid method that is suitable for on-demand testing for influenza viral infection...
  81. ncbi Visual detection of pandemic influenza A H1N1 Virus 2009 by reverse-transcription loop-mediated isothermal amplification with hydroxynaphthol blue dye
    Xue Jun Ma
    National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Ying Xin Road 100, Xuan Wu District, Beijing 100052, China
    J Virol Methods 167:214-7. 2010
    ..RT-LAMP with HNB dye was demonstrated to be a sensitive and easy assay for rapid detection of pandemic influenza A H1N1 virus...
  82. ncbi Molecular virology in the clinical laboratory
    Deborah Josko
    Department of Clinical Laboratory Sciences, Medical Laboratory Science Program, University of Medicine and Dentistry of New Jersey, 1776 Raritan Road, Scotch Plains, NJ 07076, USA
    Clin Lab Sci 23:231-6. 2010
  83. pmc Highly sensitive detection of the group A Rotavirus using Apolipoprotein H-coated ELISA plates compared to quantitative real-time PCR
    Cornelia Adlhoch
    Robert Koch Institute, Center for Biological Security ZBS1, Nordufer 20, 13353 Berlin, Germany
    Virol J 8:63. 2011
    ..In order to demonstrate that this is a valid approach we show detection of group A rotaviruses from stool samples as a proof of principle for a new method of capture ELISA that should also be applicable to other viruses...
  84. pmc Quantification of HBsAg: basic virology for clinical practice
    Jung Min Lee
    Department of Internal Medicine, Yonsei University College of Medicine, Seoul 120 752, South Korea
    World J Gastroenterol 17:283-9. 2011
    ..In this article, we review current concepts and issues on the quantification of HBsAg titers with respect to their biologic nature, method principles, and clinically relevant topics...
  85. pmc Comparison of the Eragen Multi-Code Respiratory Virus Panel with conventional viral testing and real-time multiplex PCR assays for detection of respiratory viruses
    Max Q Arens
    Department of Pediatrics, Washington University School of Medicine, St Louis, MO 63110, USA
    J Clin Microbiol 48:2387-95. 2010
    High-throughput multiplex assays for respiratory viruses are an important step forward in diagnostic virology. We compared one such assay, the PLx Multi-Code Respiratory Virus Panel (PLx-RVP), manufactured by Eragen Biosciences, Inc...
  86. pmc Simultaneous detection of influenza A, influenza B, and respiratory syncytial viruses and subtyping of influenza A H3N2 virus and H1N1 (2009) virus by multiplex real-time PCR
    Yu Chen
    Center of Clinical Laboratory, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China
    J Clin Microbiol 49:1653-6. 2011
  87. ncbi Genome analysis and detection of a Chilean isolate of Grapevine leafroll associated virus-3
    Esteban A Engel
    Fundación Ciencia para la Vida and Instituto Milenio de Biología Fundamental y Aplicada, Zañartu, 1482 Santiago, Chile
    Virus Genes 37:110-8. 2008
  88. ncbi High-content screening to distinguish between attachment and post-attachment steps of human cytomegalovirus entry into fibroblasts and epithelial cells
    Yvonne Ibig-Rehm
    Lead Finding Platform, Novartis Institutes for BioMedical Research, Novartis Campus, Basle, Switzerland
    Antiviral Res 89:246-56. 2011
    ..Compounds were identified that were able to inhibit virus entry into both cell types at either attachment or post-attachment steps...
  89. ncbi Post-genomic virology: the impact of bioinformatics, microarrays and proteomics on investigating host and pathogen interactions
    P Kellam
    Wohl Virion Centre, Department of Immunology and Molecular Pathology, Windeyer Institute of Medical Sciences, University College London, 46 Cleveland Street, London W1T 4JF, UK
    Rev Med Virol 11:313-29. 2001
    ..Such research should ultimately result in new methods for diagnosing and treating infectious diseases...
  90. pmc Posttreatment human papillomavirus testing for recurrent cervical intraepithelial neoplasia: a systematic review
    Benjamin K S Chan
    Oregon Evidence Based Practice Center, Department of Medical Informatics and Clinical Epidemiology, Oregon Health and Science University, Portland, OR, USA
    Am J Obstet Gynecol 200:422.e1-9. 2009
  91. ncbi International naming of viruses - a digest of recent developments
    L Bos
    Plant Research International WUR, Wageningen, The Netherlands
    Arch Virol 147:1471-7. 2002
  92. ncbi Constantin Levaditi (1874-1953): a pioneer in Immunology and Virology
    George Kalantzis
    Department of History of Medicine, Medical School of the Athens University, Greece
    J Med Biogr 14:178-82. 2006
  93. ncbi Comparison of the PapilloCheck® assay with the digene HC2 HPV DNA assay for the detection of 13 high-risk human papillomaviruses in cervical and anal scrapes
    Marie Noëlle Didelot
    Department of Biology and Pathology, Montpellier University Hospital, Montpellier, France
    J Med Virol 83:1377-82. 2011
    ..The results obtained indicate that the PapilloCheck® assay may be considered as a reliable screening test for HPV detection and typing...
  94. ncbi Timely diagnosis of respiratory tract infections: evaluation of the performance of the Respifinder assay compared to the xTAG respiratory viral panel assay
    M Raymaekers
    Clinical Laboratory, Jessa Hospital, Site Virga Jesse, Stadsomvaart 11, B 3500 Hasselt, Belgium
    J Clin Virol 52:314-6. 2011
    ..Existing non-molecular diagnostic methods have several drawbacks such as limited sensitivity, long turn-a-round time and limited number of pathogens that can be detected...
  95. ncbi Evaluation of the performance of the novel PapilloCheck HPV genotyping test by comparison with two other genotyping systems and the HC2 test
    Betti Schopp
    Sektion Experimentelle Virologie, Institute of Medical Virology, University Hospital Tuebingen, Tuebingen, Germany
    J Med Virol 82:605-15. 2010
    ..In conclusion, this study shows that the PapilloCheck give comparable results to established PCR methods. However, these results also show a necessity for the standardization of genotype-specific HPV detection assays...
  96. ncbi Molecular epidemiology of human papillomavirus genotype in women with high-grade squamous intraepithelial lesion and cervical cancer: will a quadrivalent vaccine be necessary in Thailand?
    Chonlaphat Sukasem
    Faculty of Medicine, Unit of Virology and Molecular Microbiology, Department of Pathology, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand
    J Med Virol 83:119-26. 2011
    ..Therefore, HPV-58, HPV-33, and HPV-68 should be considered for development of the next vaccine generation in Thailand...
  97. ncbi Detection and typing of low-risk human papillomavirus genotypes HPV 6, HPV 11, HPV 42, HPV 43 and HPV 44 by polymerase chain reaction and restriction fragment length polymorphism
    Polona J Maver
    Institute of Microbiology and Immunology, Faculty of Medicine, University of Ljubljana, Zaloska 4, 1105 Ljubljana, Slovenia
    J Virol Methods 169:215-8. 2010
    ..The novel assay is simple and robust, does not require any sophisticated equipment and can be of great value for epidemiological studies, particularly in settings in which financial resources are limited...
  98. ncbi Personal historical chronicle of six decades of basic and applied research in virology, immunology, and vaccinology
    M R Hilleman
    Merck Institute for Therapeutic Research, Merck Research Laboratories, West Point, PA 19486, USA
    Immunol Rev 170:7-27. 1999
    ..a microbe hunter in fact, was asked to chronicle his six decades of pioneering achievements in basic and applied virology, bacteriology, immunology, molecular biology, epidemiology, and cancer, with special reference to the pioneering ..
  99. pmc Standardization of sensitive human immunodeficiency virus coculture procedures and establishment of a multicenter quality assurance program for the AIDS Clinical Trials Group. The NIH/NIAID/DAIDS/ACTG Virology Laboratories
    F B Hollinger
    Division of Molecular Virology, Baylor College of Medicine, Houston, Texas 77030
    J Clin Microbiol 30:1787-94. 1992
    ..This consensus protocol is recommended for any laboratory attempting to isolate HIV for the purpose of standardizing recovery and for accessing virologic endpoints in clinical trials...
  100. ncbi A Simple-Probe real-time PCR assay for genotyping reassorted and non-reassorted isolates of Crimean-Congo hemorrhagic fever virus in southern Africa
    Kulsum Kondiah
    Department of Biotechnology, University of Johannesburg, PO Box 17011, Doornfontein 2028, South Africa
    J Virol Methods 169:34-8. 2010
    ..This assay provides a reliable and sensitive tool for the differentiation between reassorted and non-reassorted variants of CCHFV which finds application for diagnosis and epidemiologic and surveillance studies...
  101. pmc High specificity of line-immunoassay based algorithms for recent HIV-1 infection independent of viral subtype and stage of disease
    Jorg Schupbach
    University of Zurich, Institute of Medical Virology, Swiss National Center for Retroviruses, Zurich, Switzerland
    BMC Infect Dis 11:254. 2011
    ..Here, we sought to further investigate the diagnostic specificity of various Inno-Lia algorithms and to identify factors affecting it...

Research Grants69

  1. Regulation of HSV-1 Gene Expression and Replication by Nuclear Hormone Receptors
    SHAOCHUNG VICTOR HSIA; Fiscal Year: 2013
    ..a land-grant, historically black college and university (HBCU) so the students can study the current progress of virology, molecular biology, cell biology, neuroscience, and endocrinology...
  2. Novel DNA-Launched Attenuated Vaccine for VEE Virus
    PETER M PUSHKO; Fiscal Year: 2012
    ..Safety of i-DNA-derived viruses will be evaluated in mice in collaboration with the Institute of Human Virology, University of Maryland...
  3. Viral and Host Determinants of New World Alphaviral Entry
    ASIM AMINSHARIF AHMED; Fiscal Year: 2013
    ..research aims and experiments, the candidate's career development goals are to gain expertise in basic virology, viral pathogenesis, protein structure-function, high throughput genetic screens and the ethical conduct of ..
  4. Mechanisms of Neutralizing Antibody Resistance in Chronic HCV and HIV Infection
    JUSTIN RICHARD BAILEY; Fiscal Year: 2013 the Infectious Diseases Division at Johns Hopkins Hospital and an internationally- recognized expert in HCV virology, humoral immunity, and computational biology and Dr. James E. Crowe, Jr...
  5. Thirteenth Annual Meeting of the Rocky Mountain Virology Association
    Joel Rovnak; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): The Rocky Mountain Virology Club (RMVC, aka RMVA) was established in 2000 to facilitate free and open exchange of scientific data and ideas concerning general virology in a venue that promotes ..
  6. Mechanisms of Early Growth Response Factor 1 (Egr-1) Induction by HSV-1 Lytic Inf
    SHAOCHUNG VICTOR HSIA; Fiscal Year: 2010 to establish an active research program at the University of Louisiana Monroe (ULM) so the students can study Virology since our laboratory is the only research program at ULM and Northeast Louisiana dedicating to virus research...
  7. Hepatic Lipase and HCV Infection
    Tianyi Wang; Fiscal Year: 2010
    ..vaccine and alternative therapies, a goal that cannot be accomplished without a better understanding of the HCV virology. Interestingly, recent studies have linked host cholesterol and fatty acid metabolic pathways to HCV life cycle...
  8. Novel Therapeutics for Neurotropic Alphaviruses
    David J Miller; Fiscal Year: 2013
    ..development, and we have assembled a team of experienced investigators with diverse expertise in the fields of virology, neurology, pathology, physiology, and medicinal chemistry to accomplish this task...
  9. Protective orthopox immunization in normals and patients with cancer or eczema
    Ellis Reinherz; Fiscal Year: 2009
    ..Here, four groups of investigators will utilize their considerable talents in vaccinology, virology, immunology, cutaneous biology, structure and bioinformatics to identify critical orthopox epitopes affording ..
  10. The function and regulation of IL-33 in the airway epithelium in asthma
    ERIN D GORDON; Fiscal Year: 2013
    ..interested in research science as an undergraduate at the University of California, Berkeley where I worked in a virology laboratory for three years...
  11. Mechanistic Studies of HCV E2
    Joseph Marcotrigiano; Fiscal Year: 2013
    ..more in-depth mechanistic dissection of E2 requires complementary biochemical, biophysical and molecular virology techniques...
  12. Microneedle-based immunization against pandemic influenza
    Mark R Prausnitz; Fiscal Year: 2011
    ..out by a collaborative team of five Lead Investigators, including experts in microfabrication, drug delivery, virology, and immunology, with guidance from scientific and industry advisory boards...
  13. Molecular Biology of Eukaryotic Viruses
    Bert L Semler; Fiscal Year: 2012
    ..The goal of this program is to train Ph.D. graduate students in the fundamental aspects of molecular virology as they relate to the regulation of gene expression, virus structure, virus-host interactions, and pathogenesis...
  14. Biophysics of Drug Interactions with Viral Episomes
    James K Bashkin; Fiscal Year: 2013
    ..collaboration has begun around biophysical spectroscopy, DNA binding/footprinting assays, synthetic chemistry, virology and cellular-molecular biology...
  15. Regional Biocontainment Laboratory
    Charles Bailey; Fiscal Year: 2007
    ..personnel with national reputations and extensive experience in biological defense, aerobiology, bacteriology, virology, and animal model development; its location in the Washington, DC metropolitan area; and its collaborative ..
  16. Activity-dependent phosphorylation of MeCP2
    DANIEL HIGGINS EBERT; Fiscal Year: 2013
    ..Though I was excited about new research in virology, I was drawn to the frontiers of neuroscience and the clinical discipline of psychiatry...
  17. The functions of the US3 protein kinase of herpes simplex virus
    Bernard Roizman; Fiscal Year: 2011
    ..The mechanisms by which viruses conquer human cells are key objectives of virology today...
  18. Immune Regulation of Virus Clearance and Tissue Injury at Sites of Infection
    Thomas J Braciale; Fiscal Year: 2013
    ..with unique expertise and diverse and complementary skills in the areas of Viral Immunology, Mammalian Genetics, Virology and Clinical Medicine...
  19. Mechanisms of Virus Replication and Gene Expression
    ANNE ELIZABETH SIMON; Fiscal Year: 2013
    Research in Virology requires more than a focused understanding of human pathogens. It requires comprehensive knowledge of viruses that infect hosts ranging from simple eukaryotes to complex multicellular organisms...
  20. Key determinants of dengue virus neutralization by naturally occurring human mAbs
    Scott A Smith; Fiscal Year: 2013
    ..determining fine details of the dengue virus neutralizing immune response, I will acquire skills in the areas of virology, immunology, genetics and recombinant antibody techniques...
    Donald Gilden; Fiscal Year: 2013 neurology (including pediatric neurology), pediatrics or neuropathology;and (b) PhDs, with a background in virology or molecular biology...
  22. ASM Conference on Viral Genome Replication
    Sean P J Whelan; Fiscal Year: 2011
    ..It has been the tradition in virology to divide and conquer and there is an acute need for a different paradigm: to unify and conquer...
  23. Mechanisms by which VZV IE proteins interact with cell antiviral proteins to prev
    MARIA ACENA NAGEL; Fiscal Year: 2013
    ..Training will be enhanced with didactic courses in molecular virology, bioethics, and statistical analysis...
    Michael L Cleary; Fiscal Year: 2013
    ..cells;biochemical and molecular actions of oncoproteins and tumor suppressor proteins;tumor immunology and virology;immunogenetics and immunoregulation in human and murine model systems...
  25. Novel vaccine against Norovirus
    Xi Jiang; Fiscal Year: 2013
    ..This project will be conducted by a research team with multidiscipline experts in molecular virology, immunology, vaccine development at the Cincinnati Children's Hospital Medical Center (CCHMC), the Virginia ..
    DALE LESLIE GREINER; Fiscal Year: 2013
    ..The Program will be supported by a Virology and Technology Core that will provide viruses, virological and immunological analyses and new technologies to ..
  27. Strain Specific Detection of Influenza at the Point-of-Care
    HYONGSOK TOM SOH; Fiscal Year: 2012
    ..a solution to this important problem, this group of three PI's with complementary skill sets (influenza virology, aptamer biochemistry and microfluidic device engineering) propose to develop a powerful, field- portable, ..
    Marcia B Goldberg; Fiscal Year: 2012
    ..pathogenesis, genetics, toxins, virulence factors, cell biology, host defense, and vaccine development;(2) virology, including pathogenesis, genetics, cell biology, virulence, host defense, antiviral agents, and retrovirology;(3) ..
  29. Glioma immunotherapy with strategies based on autonomous parvovirus MVMp
    JUSTIN COBB PAGLINO; Fiscal Year: 2013
    ..referee) in the department of Laboratory Medicine at Yale equipped me with skills in a variety of molecular virology techniques, developed my ability to design an effective experimental approach, and resulted in two first-author ..
  30. Mechanisms of pox virus motility and release
    Daniel Kalman; Fiscal Year: 2012
    ..To do this, we will use state of the art microscopy, mass spectroscopy, cell biology, and virology methods together with lead drug compounds in an animal model...
  31. The Ecology, Emergence and Pandemic Potential of Nipah virus in Bangladesh
    Peter Daszak; Fiscal Year: 2012
    ..We will conduct 5 years of fieldwork in Bangladesh, collecting data on bat ecology, virology, demography and social behavior...
  32. 2nd ASM Conference on Manipulation of Nuclear Processes by DNA Viruses
    Laimonis A Laimins; Fiscal Year: 2011
    ..This is a significant area in virology that is yielding many new and exciting insights into viral pathogenesis...
  33. Spatiotemporal Regulation of Membrane Raft Trafficking in Virus Activated Cells
    Tione Buranda; Fiscal Year: 2012
    ..brings together a unique team employing tools and concepts of biophysical science, molecular cell biology and virology to study the mechanism used by hantaviruses to enter cells...
  34. LTQ Orbitrap XL ETD Mass Spectrometer
    Haining Zhu; Fiscal Year: 2010
    ..including cutting-edge programs focused on neurological disorders, cancer, diabetes, cardiovascular disease, virology, eye diseases, and women's health...
  35. Novel Inhibitors of Viral Replication
    Michael G Roth; Fiscal Year: 2013
    ..We will combine chemical biology, cell biology, and virology to investigate the mechanisms by which these compounds inhibit virus replication with the twin goals of ..
  36. Norovirus P Particle, A Multifunctional Platform For Vaccine Development
    Ming Tan; Fiscal Year: 2012
    ..Cincinnati Children's Hospital Medical Center with expertises in molecular biology, structural biology, and virology, immunology and vaccine technology...
  37. Enteric Virus Calcium Channel Inhibitors
    Joseph M Hyser; Fiscal Year: 2013
    ..research program on viral calcium channels and development of viroporin inhibitors as tools for molecular virology studies and leads for antiviral drug development. My two mentors, Dr. Frank Horrigan and Dr...
  38. Assembly dynamics and role of PI4P enriched replication organelles for enterovira
    Ann Cali; Fiscal Year: 2013
    ..Our findings have brought forward a new paradigm to virology;revealing how viruses can selectively exploit specific elements of the host to form specialized organelles where ..
  39. Novel Tetravalent Vaccines for Dengue Virus
    ROBERT GERALD WHALEN; Fiscal Year: 2013
    ..To assist us in this work, we have assembled a team of consul- tants and collaborators with expertise in many different fields of dengue virology, immunology, and pathology.
  40. Development of the Cotton Rat Model of Rhinovirus Infection and Disease
    JORGE C BLANCO; Fiscal Year: 2013 of investigators has been assembled combining extensive expertise in the use of cotton rats and strong virology capabilities...
  41. Benzophenones: Novel natural agents which inhibits HIV and Candida
    Ramiro Mendonca Murata; Fiscal Year: 2011
    ..This training will provide expertise in virology, structure determination, and cell culture techniques...
  42. Benzophenones: Novel natural agents which inhibits HIV and Candida
    Ramiro Mendonca Murata; Fiscal Year: 2013
    ..This training will provide expertise in virology, structure determination, and cell culture techniques...
  43. Training in Hantavirus Ecology, Virology & Clinical Investigation in the Americas
    GREGORY JAMES MERTZ; Fiscal Year: 2010
    ..the Western Hemisphere in 1993, so there is an urgent need to build expertise in the Americas in rodent ecology, virology, clinical care, and clinical research as it relates to hantavirus infections in the Americas. Our group of U.S...
  44. Genetic determinants of HSV-1 virulence in neurons
    Moriah L Szpara; Fiscal Year: 2013
    ..pathogens, based on the strong foundations of my graduate training in neurobiology and postdoctoral training in virology. This award will facilitate my career development by allowing me to gain training in bioinformatics and ..
    JACK J HAWIGER; Fiscal Year: 2010
    ..from a pool of graduate and MD/PhD students who matriculated to VU and MMC and have indicated microbiology, virology, immunology, and/or molecular cell biology as their area(s) of interest...
  46. UCB/UCSF AIDS International Training and Research Program (AITRP)
    Arthur L Reingold; Fiscal Year: 2013
    ..behavioral and policy research, including study design and data collection, management, and analysis;virology, immunology, serology, and other diagnostic methods related to AIDS/HIV;clinical microbiology related to STDs, TB,..
  47. Vertical CMV transmission in the setting of maternal HIV-1 infection in Kenya
    JENNIFER ANN SLYKER; Fiscal Year: 2013
    ..which will be combined with mentorship in epidemiology, statistical analyses, clinical trials design, and CMV virology which will enable the candidate to develop an independent research career in molecular epidemiology...
  48. Dengue Virus Genome Encapsidation and Its Interplay with Host Lipid Droplets
    Andrea Gamarnik; Fiscal Year: 2013
    ..In this proposal, we will combine molecular biology, biochemistry, and genetic approaches with classical virology studies to define the mechanism of dengue virus encapsidation and its interplay with the host cell...
  49. TIM recognition of phosphatidylserine regulates innate and adaptive immunity
    Dale T Umetsu; Fiscal Year: 2013
    ..monkeys and complementary approaches involving crystallogra[unreadable][unreadable] phy, structural biology, virology, biochemistry as well as cellular and molecular immunology, in models of asthma, hepatitis, respiratory tolerance ..
  50. Global Health Partnership at UCSD and SDSU
    Steffanie Strathdee; Fiscal Year: 2009
    ..behavioral science, bioterrorism, anthropology, economics, political science, geography, sociology, toxicology, virology, parasitology and molecular biology. Teams of researchers at our universities have a history of working together...
  51. HIV Nucleoside Analogs Translational Studies: Resistance and Metabolism
    Elijah Paintsil; Fiscal Year: 2012
    ..The candidate's goal is to develop a command of virology and pharmacology by the integration of research studies on the cellular pharmacology of HIV reverse transcriptase ..
  52. Opening a New Lassa Fever Ward in Kenema Sierra Leone
    Robert F Garry; Fiscal Year: 2013
    ..We will discuss the many recent breakthroughs in our understanding of the virology, immunology, epidemiology and natural history of Lassa fever...
  53. XV International Conference on Human Retrovirology: HTLV and Related Viruses
    Edward L Murphy; Fiscal Year: 2011
    ..disease research institutions gather at this biennial meeting to present their latest data on the molecular virology, immunology, epidemiology and clinical outcomes of HTLV infection...
  54. Human Herpesvirus-8 Replication and Kaposi Sarcoma Response to Treatment
    Warren Phipps; Fiscal Year: 2013
    ..Corey Casper, Larry Corey, and Anna Wald, who provide unparalleled expertise in herpesvirus virology and infection-related cancers...
  55. Human neutralizing monoclonal antibodies for Rift Valley fever virus
    James E Crowe; Fiscal Year: 2013
    ..This proposal incorporates experts in molecular immunology and RVF virology and viral pathogenesis with access to CDC/USDA approved BSL-3 enhanced (BSL3+) facilities...
  56. City of Hope Clinical Oncology Career Research Development Program
    Joanne E Mortimer; Fiscal Year: 2013
    ..goals, the enhancement of interactive basic and clinical research at the cutting edge of molecular genetics, virology and gene therapy, immunology and immunotherapy, bone marrow transplantation and stem cell biology, and ..
  57. One Health;Epidemiology of natural and deliberate contaminants (infectious and t
    Susan Sanchez; Fiscal Year: 2013
    ..Our laboratory has expertise in the areas of pathology, bacteriology, parasitology, virology, serology, and molecular biology, including high throughput platform testing...
  58. Stimulation of broadly neutralizing antibodies against influenza in a pig model
    RADHEY SHYAM KAUSHIK; Fiscal Year: 2011
    ..of David Francis (Co-PI, Pig model), Radhey Kaushik (Co-PI, swine immunology), Feng Li (Investigator, influenza virology) and two swine influenza consultants (Weijun Ma and Mahesh Khatri)...
  59. Behavioral and Virologic Impact of HPV Immunization
    Jessica A Kahn; Fiscal Year: 2012
    ..impact of type-specific HPV vaccination is an important but under-investigated area of behavioral science and virology that has direct implications for maximizing the public health impact of HPV vaccines...
  60. The Structure and Function of the Influenza Virus Genome
    Robert A Lamb; Fiscal Year: 2013
    ..using assays that are well established in the field of membrane biophysics but are under utilized in the field of virology and that we have developed in our laboratory. We will analyze virus assembly in vivo by isolating membrane sheets...
  61. 2010 Molecular Biology of Hepatitis B Viruses Meeting
    Adam Zlotnick; Fiscal Year: 2010
    ..Since then, the HBV meeting has introduced a continuous series of broad-reaching advances to the virology community...
  62. Interdisciplinary Training in Virology and Gene Therapy
    Ren Sun; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Developments in virology have led to the advancement of several disciplines, including gene therapy. Gene therapy holds promise of therapeutic options for a variety of diseases...
    Nigel Fraser; Fiscal Year: 2007
    ..We now wish to continue to refine these models using the techniques of molecular virology in order to identify drug targets to intervene in the latency process...
    Nelson Fausto; Fiscal Year: 1999
    ..This application represents the combined efforts of investigators with expertise in clinical virology (HCV quantitation and subspecies characterization), the development of differentiated hepatocyte cell lines and ..
    Celia A Schiffer; Fiscal Year: 2012
    ..AIDS-related research activities at UMASS are supported by Clinical (Core C), Molecular Virology (Core D) and Molecular Biology (Core E) Cores...
  66. 2009 NHP Models for AIDS Conference
    Amitinder Kaur; Fiscal Year: 2009
    ..The latest findings in primate pathogenesis, immunology, genomics, virology, vaccines and therapeutics will be presented...
    ROLAND RUECKERT; Fiscal Year: 1990
    ..outstanding junior American virologists to attend and participate in the Eighth International Congress of Virology (ICV) to be held August 1990 in Berlin, West Germany...
  68. Plant-derived vaccines against hepatitis C Cooperative *
    Charles Arntzen; Fiscal Year: 2009
    DESCRIPTION (provided by applicant): Research in immunology and virology has made enormous progress toward the design of an effective vaccine to prevent Hepatitis C infection, but multidisciplinary efforts are needed to move these ..
    Hilary Koprowski; Fiscal Year: 1999
    Increasingly molecularly oriented virology and genetic studies in this program of the last fifteen years have brought us to the point that our main theme in this revised renewal program is to use molecular techniques in virology and ..