pentose phosphate pathway

Summary

Summary: An oxidative decarboxylation process that converts GLUCOSE-6-PHOSPHATE to D-ribose-5-phosphate via 6-phosphogluconate. The pentose product is used in the biosynthesis of NUCLEIC ACIDS. The generated energy is stored in the form of NADPH. This pathway is prominent in tissues which are active in the synthesis of FATTY ACIDS and STEROIDS.

Top Publications

  1. ncbi Glucose metabolism in Chlamydia trachomatis: the 'energy parasite' hypothesis revisited
    E R Iliffe-Lee
    Department of Medical Microbiology, University of Manitoba, 730 William Avenue, Winnipeg, Manitoba, Canada R3E 0W3
    Mol Microbiol 33:177-87. 1999
  2. ncbi Starch biosynthesis and intermediary metabolism in maize kernels. Quantitative analysis of metabolite flux by nuclear magnetic resonance
    Erich Glawischnig
    , , Am Hochanger 8, 85350 Freising, Germany
    Plant Physiol 130:1717-27. 2002
  3. ncbi Glucose-6-phosphate dehydrogenase: a novel therapeutic target in cardiovascular diseases
    Sachin A Gupte
    University of South Alabama, College of Medicine, Department of Biochemistry and Molecular Biology, MSB 2310, 307 University Boulevard North, Mobile, AL 36688, USA
    Curr Opin Investig Drugs 9:993-1000. 2008
  4. ncbi Defective carbohydrate metabolism in mice homozygous for the tubby mutation
    Yun Wang
    The Jackson Laboratory, Bar Harbor, Maine 04609, USA
    Physiol Genomics 27:131-40. 2006
  5. ncbi 13C-labeled gluconate tracing as a direct and accurate method for determining the pentose phosphate pathway split ratio in Penicillium chrysogenum
    Roelco J Kleijn
    Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands
    Appl Environ Microbiol 72:4743-54. 2006
  6. ncbi Modulation of astroglial energy metabolism by nitric oxide
    Juan P Bolanos
    Departamento de Bioquimica y Biologia Molecular, Universidad de Salamanca Instituto de Neurociencias de Castilla y León, Centro Nacional de Investigaciones Cardiovasculares, Campus Miguel de Unamuno, Salamanca, Spain
    Antioxid Redox Signal 8:955-65. 2006
  7. ncbi Interpretation of metabolic flux maps by limitation potentials and constrained limitation sensitivities
    S Aljoscha Wahl
    Institute of Biotechnology 2, , , Germany
    Biotechnol Bioeng 94:263-72. 2006
  8. ncbi Metabolic profiling by 13C-NMR spectroscopy: [1,2-13C2]glucose reveals a heterogeneous metabolism in human leukemia T cells
    A Miccheli
    Department of Chemistry, University of Rome La Sapienza, P.le Aldo Moro 5, 00185 Rome, Italy
    Biochimie 88:437-48. 2006
  9. ncbi Leishmania major and Leishmania tropica: II. Effect of an immunomodulator, S(2) complex on the enzymes of the parasites
    Yassir M Al-Mulla Hummadi
    Department of Pharmacology, College of Medicine, University of Baghdad, P.O. Box 61208, Baghdad 12114, Iraq
    Exp Parasitol 112:85-91. 2006
  10. ncbi K-ras codon-specific mutations produce distinctive metabolic phenotypes in NIH3T3 mice [corrected] fibroblasts
    Pedro Vizan
    Department of Biochemistry and Molecular Biology, Faculty of Biology, University of Barcelona, Spain
    Cancer Res 65:5512-5. 2005

Detail Information

Publications62

  1. ncbi Glucose metabolism in Chlamydia trachomatis: the 'energy parasite' hypothesis revisited
    E R Iliffe-Lee
    Department of Medical Microbiology, University of Manitoba, 730 William Avenue, Winnipeg, Manitoba, Canada R3E 0W3
    Mol Microbiol 33:177-87. 1999
    ..functional enzymes that produce energy or reducing power, four enzymes involved in glycolysis or the pentose phosphate pathway, specifically pyruvate kinase, phosphoglycerate kinase, glyceraldehyde-3-phosphate dehydrogenase and ..
  2. ncbi Starch biosynthesis and intermediary metabolism in maize kernels. Quantitative analysis of metabolite flux by nuclear magnetic resonance
    Erich Glawischnig
    , , Am Hochanger 8, 85350 Freising, Germany
    Plant Physiol 130:1717-27. 2002
    ..The relatively high abundance of the [5,6-(13)C(2)]-isotopomer can be explained by the joint operation of glycolysis/glucogenesis and the pentose phosphate pathway.
  3. ncbi Glucose-6-phosphate dehydrogenase: a novel therapeutic target in cardiovascular diseases
    Sachin A Gupte
    University of South Alabama, College of Medicine, Department of Biochemistry and Molecular Biology, MSB 2310, 307 University Boulevard North, Mobile, AL 36688, USA
    Curr Opin Investig Drugs 9:993-1000. 2008
    ..This warrants the development of new drugs or genetic approaches to target G6PD for investigational and clinical use. This review discusses the specificity and side effects of existing investigational G6PD inhibitors...
  4. ncbi Defective carbohydrate metabolism in mice homozygous for the tubby mutation
    Yun Wang
    The Jackson Laboratory, Bar Harbor, Maine 04609, USA
    Physiol Genomics 27:131-40. 2006
    ..tubby mice fail to induce glucose-6-phosphate dehydrogenase (G6pdh), the rate-limiting enzyme in the pentose phosphate pathway that normally supplies NADPH for de novo fatty acid synthesis and glutathione reduction...
  5. ncbi 13C-labeled gluconate tracing as a direct and accurate method for determining the pentose phosphate pathway split ratio in Penicillium chrysogenum
    Roelco J Kleijn
    Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands
    Appl Environ Microbiol 72:4743-54. 2006
    In this study we developed a new method for accurately determining the pentose phosphate pathway (PPP) split ratio, an important metabolic parameter in the primary metabolism of a cell...
  6. ncbi Modulation of astroglial energy metabolism by nitric oxide
    Juan P Bolanos
    Departamento de Bioquimica y Biologia Molecular, Universidad de Salamanca Instituto de Neurociencias de Castilla y León, Centro Nacional de Investigaciones Cardiovasculares, Campus Miguel de Unamuno, Salamanca, Spain
    Antioxid Redox Signal 8:955-65. 2006
    ..The stimulation of these glucose-metabolizing pathways by NO would represent a transient attempt by the glial cells to compensate for energy impairment and oxidative stress, and thus to emerge from an otherwise pathological outcome...
  7. ncbi Interpretation of metabolic flux maps by limitation potentials and constrained limitation sensitivities
    S Aljoscha Wahl
    Institute of Biotechnology 2, , , Germany
    Biotechnol Bioeng 94:263-72. 2006
    ..It can be shown that the reachable yield is drastically reduced by the measured efflux into the TCA cycle, while the oxidative pentose-phosphate pathway only plays a secondary role on the reachable maximum...
  8. ncbi Metabolic profiling by 13C-NMR spectroscopy: [1,2-13C2]glucose reveals a heterogeneous metabolism in human leukemia T cells
    A Miccheli
    Department of Chemistry, University of Rome La Sapienza, P.le Aldo Moro 5, 00185 Rome, Italy
    Biochimie 88:437-48. 2006
    ..and ribose-5-phosphate moiety of nucleotides has allowed original integrated information regarding the pentose phosphate pathway, TCA cycle, and amino acid metabolism in proliferating human leukemia T cells to be obtained...
  9. ncbi Leishmania major and Leishmania tropica: II. Effect of an immunomodulator, S(2) complex on the enzymes of the parasites
    Yassir M Al-Mulla Hummadi
    Department of Pharmacology, College of Medicine, University of Baghdad, P.O. Box 61208, Baghdad 12114, Iraq
    Exp Parasitol 112:85-91. 2006
    ..Thus, the direct antileishmanial effect of S(2) complex may result, partially or entirely, from the inhibition of enzymes that are necessary for the parasites' carbohydrate metabolism...
  10. ncbi K-ras codon-specific mutations produce distinctive metabolic phenotypes in NIH3T3 mice [corrected] fibroblasts
    Pedro Vizan
    Department of Biochemistry and Molecular Biology, Faculty of Biology, University of Barcelona, Spain
    Cancer Res 65:5512-5. 2005
    ..along pathways that produce nucleic acid and lipid synthesis precursors in the oxidative branch of the pentose phosphate pathway and via pyruvate dehydrogenase flux...
  11. ncbi Quantitative estimation of channeling from early glycolytic intermediates to CO in intact Escherichia coli
    Georgia Shearer
    Department of Biology, Washington University, St. Louis, MO 63130, USA
    FEBS J 272:3260-9. 2005
    ..The data also suggest that, in addition to pathway enzymes being associated with one another, some are free in the aqueous cytosol. How sensitive the degree of channeling is to growth or experimental conditions remains to be determined...
  12. ncbi Theoretical aspects of 13C metabolic flux analysis with sole quantification of carbon dioxide labeling
    Tae Hoon Yang
    Biochemical Engineering, Saarland University, Bldg. 2, , Germany
    Comput Biol Chem 29:121-33. 2005
    ..Metabolic net fluxes in the central metabolism, involving, e.g. glycolysis, pentose phosphate pathway, tricarboxylic acid cycle, anaplerotic carboxylation, and glyoxylate pathway were found to be ..
  13. ncbi Metabolic-flux and network analysis in fourteen hemiascomycetous yeasts
    Lars M Blank
    Institute of Biotechnology, Building HPT E58, ETH Zurich, 8093 Zurich, Switzerland
    FEMS Yeast Res 5:545-58. 2005
    ..In most yeasts, the PP pathway activity appears to be driven exclusively by the demand for NADPH...
  14. ncbi Intracellular metabolite profiling of Fusarium oxysporum converting glucose to ethanol
    Gianni Panagiotou
    Center for Microbial Biotechnology, BioCentrum-DTU, Technical University of Denmark, Soltofts Plads, Building 223, 2800 Lyngby, Denmark
    J Biotechnol 115:425-34. 2005
    ..characterized by high efflux of glyceraldehyde-3-phosphate (G3P) and fructose-6-phosphate (F6P) from the pentose phosphate pathway (PPP) to the Embden-Meyerhof-Parnas (EMP) pathway, resulting in the highest ethanol production under ..
  15. ncbi Metabolic fluxes in Corynebacterium glutamicum during lysine production with sucrose as carbon source
    Christoph Wittmann
    Biochemical Engineering, Saarland University, Saarbrucken, Germany
    Appl Environ Microbiol 70:7277-87. 2004
    ..7% (normalized to an uptake flux of hexose units of 100%) through the pentose phosphate pathway (PPP). The glucose monomer of sucrose was completely channeled into the PPP...
  16. ncbi Futile cycling of lactate through the plasma membrane of C6 glioma cells as detected by (13C, 2H) NMR
    Tiago B Rodrigues
    Laboratory for Imaging and Spectroscopy by Magnetic Resonance, , C/Arturo Duperier 4, E-28029 Madrid, Spain
    J Neurosci Res 79:119-27. 2005
    ....
  17. ncbi Aluminum toxicity triggers the nuclear translocation of HIF-1alpha and promotes anaerobiosis in hepatocytes
    Ryan J Mailloux
    Department of Chemistry and Biochemistry, Laurentian University, Sudbury, Ont, Canada P3E 2C6
    Toxicol In Vitro 21:16-24. 2007
    ..These findings reveal the ability of Al to create a hypoxic environment that promotes the translocation of HIF-1alpha to the nucleus and stimulates the anaerobic metabolism of D-glucose...
  18. ncbi Evaluating microarrays using a semiparametric approach: application to the central carbon metabolism of Escherichia coli BL21 and JM109
    Je Nie Phue
    Biotechnology Unit, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Building 14A, Room 170, 9000 Rockville Pike, Bethesda, MD 20892, USA
    Genomics 89:300-5. 2007
    ..the two strains, whereas no differences were apparent for the groups comprising either glycolysis or the pentose phosphate pathway. These results further characterized differences between the two E...
  19. ncbi Amyloid-beta peptide affects the oxygen dependence of erythrocyte metabolism: a role for caspase 3
    M Elisabetta Clementi
    CNR, Istituto di Chimica del Riconoscimento Molecolare, L go F Vito n 1, CAP 00168 Rome, Italy
    Int J Biochem Cell Biol 39:727-35. 2007
    ..This finding suggests that human erythrocytes following to exposure to amyloid-beta peptide show a complete loss of the oxygen-dependent metabolic modulation, which is partially restored by caspase 3 inhibitor-treatment...
  20. ncbi [2D [1H,13C] NMR study of carbon fluxes during glucose utilization by Escherichia coli MG1655]
    A D Kivero
    Prikl Biokhim Mikrobiol 44:168-75. 2008
    Carbon fluxes through main pathways of glucose utilization in Escherichia coli cells--glycolysis, pentose phosphate pathway (PPP), and Enther-Doudoroff pathway (EDP)--were studied. Their ratios were analyzed in E...
  21. ncbi Metabolic capacity estimation of Escherichia coli as a platform for redox biocatalysis: constraint-based modeling and experimental verification
    Lars M Blank
    Laboratory of Chemical Biotechnology, TU Dortmund, D 44221 Dortmund, Germany
    Biotechnol Bioeng 100:1050-65. 2008
    ..With this optimization criterion, simulations showed exclusive use of the pentose phosphate pathway at high rates of glucose catabolism, a flux distribution usually not found in wild-type cells...
  22. ncbi An analytic and systematic framework for estimating metabolic flux ratios from 13C tracer experiments
    Ari Rantanen
    Department of Computer Science, University of Helsinki, Finland
    BMC Bioinformatics 9:266. 2008
    ....
  23. ncbi Metabolic regulation of pathways of carbohydrate oxidation in potato (Solanum tuberosum) tubers
    Danilo C Centeno
    Max Planck Institut fur Molekulare Pflanzenphysiologie, Am Muhlenberg 1, 14476 Potsdam Golm, Germany
    Physiol Plant 133:744-54. 2008
    ..cv. Desiree). We analysed the relative rates of glycolysis and the oxidative pentose phosphate pathway that these lines exhibited as well as the relative contributions of the cytochrome and alternative ..
  24. ncbi Elevated activity of the oxidative and non-oxidative pentose phosphate pathway in (pre)neoplastic lesions in rat liver
    Wilma M Frederiks
    Academic Medical Center, University of Amsterdam, Department of Cell Biology and Histology, Amsterdam, The Netherlands
    Int J Exp Pathol 89:232-40. 2008
    ..are characterized by elevated activity of the first irreversible enzyme of the oxidative branch of the pentose phosphate pathway (PPP), glucose-6-phosphate dehydrogenase (G6PD), for production of NADPH...
  25. ncbi Profiling the metabolic proteome of bovine mammary tissue
    Amanda J Beddek
    Centre for Biodiscovery and School of Biological Sciences, Victoria University of Wellington, Wellington, New Zealand
    Proteomics 8:1502-15. 2008
    ..11 out of the 15 enzymes in the direct pathways for conversion of glucose to fatty acids, two of the pentose phosphate pathway enzymes and two of the enzymes for lactose synthesis from glucose...
  26. ncbi Comparison of metabolic flux distributions for MDCK cell growth in glutamine- and pyruvate-containing media
    Yury Sidorenko
    Max Planck Institute for Dynamics of Complex Technical Systems, Sandtorstrasse 1, Magdeburg, Germany
    Biotechnol Prog 24:311-20. 2008
    ..Thus, the switch from glutamine-containing to glutamine-free medium with pyruvate provided a series of benefits without dramatic changes of cellular metabolism...
  27. ncbi Metabolic flux analysis in Escherichia coli by integrating isotopic dynamic and isotopic stationary 13C labeling data
    Jochen Schaub
    Institute of Biochemical Engineering, University of Stuttgart, Allmandring 31, D 70569 Stuttgart, Germany
    Biotechnol Bioeng 99:1170-85. 2008
    ..of metabolic fluxes was developed and applied to determine intracellular fluxes in glycolysis, pentose phosphate pathway (PPP), and citric acid cycle (TCA) in Escherichia coli grown in aerobic, glucose-limited chemostat ..
  28. ncbi Retooling Leishmania metabolism: from sand fly gut to human macrophage
    Doron Rosenzweig
    Faculty of Biology, Technion Israel Institute of Technology, Haifa 32000, Israel
    FASEB J 22:590-602. 2008
    ..These changes occur while promastigotes undergo morphological transformation. Our findings provide new insight into changes occurring in single-cell organisms during a developmental process...
  29. ncbi The fate of glucose during the period of decreased metabolism after fluid percussion injury: a 13C NMR study
    Brenda L Bartnik
    UCLA Brain Injury Research Center, Division of Neurosurgery, David Geffen School of Medicine at UCLA, Los Angeles California, USA
    J Neurotrauma 24:1079-92. 2007
    ..The percentage of glucose metabolism through the pentose phosphate pathway (PPP) increased in the injured (13%) and contralateral (11%) cortex at 3...
  30. ncbi Carbohydrate metabolism in Dioscorea esculenta (Lour.) Burk. tubers and Curcuma longa L. rhizomes during two phases of dormancy
    R Panneerselvam
    Stress Physiology Laboratory, Department of Botany, Annamalai University, Annamalainagar 608002, Tamil Nadu, India
    Colloids Surf B Biointerfaces 59:59-66. 2007
    ..enzymes of starch degradation, respiration, glycolysis, tricarboxylic acid (TCA) cycle and oxidative pentose phosphate pathway (PPP) were studied during 1-70 days after harvest (DAH)...
  31. ncbi Adaptation of energy metabolism in breast cancer brain metastases
    Emily I Chen
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Cancer Res 67:1472-86. 2007
    ..The cells further showed enhanced activation of the pentose phosphate pathway and the glutathione system, which can minimize production of reactive oxygen species resulting from an ..
  32. ncbi Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for metabolic flux analyses using isotope-labeled ethanol
    Klaus Hollemeyer
    Biochemical Engineering Institute, Im Stadtwald, Bldg A 1 5, Saarland University, D 66123 Saarbrucken, Germany
    Rapid Commun Mass Spectrom 21:336-42. 2007
    ..3%...
  33. ncbi Metabolic network analysis on Phaffia rhodozyma yeast using 13C-labeled glucose and gas chromatography-mass spectrometry
    Christopher Cannizzaro
    Laboratory of Chemical and Biochemical Engineering, Swiss Federal Institute of Technology (EPFL, CH-1015 Lausanne, Switzerland
    Metab Eng 6:340-51. 2004
    ..GC-MS, were in accordance with a metabolic network consisting of the Embden-Meyerhof-Parnas pathway, the pentose phosphate pathway, and the TCA cycle...
  34. ncbi Metabolic flux analysis based on 13C-labeling experiments and integration of the information with gene and protein expression patterns
    Kazuyuki Shimizu
    Department of Biochemical Engineering and Science, Kyushu Institute of Technology, Iizuka, Fukuoka 820 8502, Japan
    Adv Biochem Eng Biotechnol 91:1-49. 2004
    ....
  35. ncbi Protein S-thiolation targets glycolysis and protein synthesis in response to oxidative stress in the yeast Saccharomyces cerevisiae
    Daniel Shenton
    Department of Biomolecular Sciences, University of Manchester Institute of Science and Technology (UMIST, P.O. Box 88, Manchester M60 1QD, UK
    Biochem J 374:513-9. 2003
    ..dehydrogenase or 6-phosphogluconate dehydrogenase, the enzymes that catalyse NADPH production via the pentose phosphate pathway. Thus the inhibition of glycolytic flux is proposed to result in glucose equivalents entering the ..
  36. ncbi The stable isotope-based dynamic metabolic profile of butyrate-induced HT29 cell differentiation
    Joan Boren
    Department of Biochemistry and Molecular Biology, , , University of Barcelona, , 08028 Barcelona, Catalonia, Spain
    J Biol Chem 278:28395-402. 2003
    ....
  37. ncbi Failure to increase glucose consumption through the pentose-phosphate pathway results in the death of glucose-6-phosphate dehydrogenase gene-deleted mouse embryonic stem cells subjected to oxidative stress
    Stefania Filosa
    IGB Adriano Buzzati Traverso CNR, Via G Marconi 12, 80125 Napoli, Italy
    Biochem J 370:935-43. 2003
    ..Moreover, bacterial G6PD can substitute for the human enzyme, strongly suggesting that a relatively simple mechanism of enzyme kinetics underlies this phenomenon...
  38. ncbi Genealogy profiling through strain improvement by using metabolic network analysis: metabolic flux genealogy of several generations of lysine-producing corynebacteria
    Christoph Wittmann
    Biochemical Engineering Institute, Saarland University, Saarbruecken, Germany
    Appl Environ Microbiol 68:5843-59. 2002
    ..The relative pentose phosphate pathway (PPP) flux successively increased, clearly corresponding to the product yield...
  39. ncbi Fermented wheat germ extract inhibits glycolysis/pentose cycle enzymes and induces apoptosis through poly(ADP-ribose) polymerase activation in Jurkat T-cell leukemia tumor cells
    Begona Comin-Anduix
    Department of Biochemistry and Molecular Biology, CeRQT-PCB at Barcelona Scientific Park, University of Barcelona, , Barcelona 08028, Spain
    J Biol Chem 277:46408-14. 2002
    ..02 mg/ml) for peripheral blood lymphocytes to induce a biological response, which provides the broad therapeutic window for this supplemental cancer treatment modality with no toxic effects...
  40. ncbi O(2)-dependent stimulation of the pentose phosphate pathway by S-nitrosocysteine in human erythrocytes
    Francesco Misiti
    Istituto di Biochimica e Biochimica Clinica, , , 00168 Rome, Italy
    Biochem Biophys Res Commun 294:829-34. 2002
    ..In particular, glucose metabolised through the pentose phosphate pathway (PPP) was higher in treated erythrocytes than in untreated cells only at high O(2) pressure...
  41. ncbi Accelerated rates of glycolysis in the hypertrophied heart: are they a methodological artifact?
    Hon Sing Leong
    McDonald Research Laboratories The iCAPTUR, University of British Columbia St Paul s Hospital, Vancouver, British Columbia, V6Z 1Y6
    Am J Physiol Endocrinol Metab 282:E1039-45. 2002
    ..However, nonglycolytic detritiation of [5-(3)H]glucose via the nonoxidative pentose phosphate pathway (PPP) could potentially lead to an overestimation of true glycolytic rates, especially in hypertrophied ..
  42. ncbi Metabolic flux responses to pyruvate kinase knockout in Escherichia coli
    Marcel Emmerling
    Institute of Biotechnology, ETH Zurich, CH 8093 Zurich, Switzerland
    J Bacteriol 184:152-64. 2002
    ..Responses in the pentose phosphate pathway and the tricarboxylic acid cycle were strongly dependent on the environmental conditions...
  43. ncbi Bacillus subtilis metabolism and energetics in carbon-limited and excess-carbon chemostat culture
    M Dauner
    Institute of Biotechnology, , , Switzerland
    J Bacteriol 183:7308-17. 2001
    ..ATP-dependent control of in vivo enzyme activity appears to be irrelevant for the observed differences in TCA cycle fluxes...
  44. ncbi Influences of pH on human platelet metabolism
    J M Baker
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT
    Platelets 12:333-42. 2001
    ..The rate of the oxidative pentose phosphate pathway also increased with increasing pH and this pathway was inhibited by both acetate and propionate...
  45. ncbi [5-3H]glucose overestimates glycolytic flux in isolated working rat heart: role of the pentose phosphate pathway
    G W Goodwin
    Division of Cardiology, University of Texas-Houston Medical School, Department of Pediatrics, Baylor College of Medicine, Houston, TX 77030, USA
    Am J Physiol Endocrinol Metab 280:E502-8. 2001
    We set out to study the pentose phosphate pathway (PPP) in isolated rat hearts perfused with [5-3H]glucose and [1-14C]glucose or [6-14C]glucose (crossover study with 1- then 6- or 6- then 1-14C-labeled glucose)...
  46. ncbi A flux model of glycolysis and the oxidative pentosephosphate pathway in developing Brassica napus embryos
    Jorg Schwender
    Department of Plant Biology, Michigan State University, East Lansing, Michigan 48824, USA
    J Biol Chem 278:29442-53. 2003
    ..metabolic flux model was developed and tested for the reaction network of glycolysis and the oxidative pentose phosphate pathway (OPPP)...
  47. ncbi Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways
    Hans-Matti Blencke
    , , , Staudtstr. 5, D-91058 Erlangen, Germany
    Metab Eng 5:133-49. 2003
    ..The genes encoding enzymes of the pentose phosphate pathway are expressed both in the presence and the absence of glucose, as suggested by the central role of this ..
  48. ncbi From genome to enzyme: analysis of key glycolytic and oxidative pentose-phosphate pathway enzymes in the cyanobacterium Synechocystis sp. PCC 6803
    Vicki L Knowles
    Department of Biology, Queen's University, Kingston, Ontario, K7L 3N6 Canada
    Plant Cell Physiol 44:758-63. 2003
    ..Immunoblot and kinetic studies indicated that irrespective of nutritional mode, the Synechocystis PK corresponds to a PK-A (AMP activated) rather than PK-F (fructose-1,6-bisphosphate activated)...
  49. ncbi Human sebaceous glands engage in aerobic glycolysis and glutaminolysis
    M M T Downie
    Department of Clinical Biochemistry, University of Cambridge, Addenbrooke s Hospital, Box 232, Hills Road, Cambridge CB2 2QR, UK
    Br J Dermatol 151:320-7. 2004
    ..The skin and its appendages support aerobic glycolytic and glutaminolytic metabolism. Their major fuels are glucose and glutamine, which are, however, largely catabolized anaerobically...
  50. ncbi Phenotypic characterization of glucose repression mutants of Saccharomyces cerevisiae using experiments with 13C-labelled glucose
    Vijayendran Raghevendran
    Centre for Microbial Biotechnology, BioCentrum-DTU, Building 223, Technical University of Denmark, Lyngby DK 2800, Denmark
    Yeast 21:769-79. 2004
    ..Furthermore, deletion of the genes MIG1, MIG1/MIG2 and REG1 did not result in a significant change in the phenotype at the fluxome level...
  51. ncbi Effects of exposure to a simulated altitude of 5500 m on energy metabolic pathways in rats
    L C Ou
    Department of Physiology, Dartmouth Medical School, Lebanon, NH 03756, USA
    Respir Physiol Neurobiol 141:59-71. 2004
    ..On the other hand, enhanced fatty acid metabolism may spare carbohydrate for metabolic fuel under conditions of extreme hypoxic limitation...
  52. ncbi The Bacillus subtilis yqjI gene encodes the NADP+-dependent 6-P-gluconate dehydrogenase in the pentose phosphate pathway
    Nicola Zamboni
    Institute of Biotechnology, ETH Zurich, Zurich, Switzerland
    J Bacteriol 186:4528-34. 2004
    ....
  53. ncbi The pentose phosphate pathway in Trypanosoma cruzi
    Dante A Maugeri
    Instituto de Investigaciones Biotecnologicas/INTECH, Universidad Nacional de General San Martin/CONICET, Av. Gral Paz s/n, INTI, Edificio 24, Av. General Paz y Albarellos, Casilla de Correo 30, 1650 San Martin, Buenos Aires, Argentina
    FEMS Microbiol Lett 234:117-23. 2004
    The pentose phosphate pathway has been studied in Trypanosoma cruzi, Clone CL Brener. Functioning of the pathway was demonstrated in epimastigotes by measuring the evolution of (14)CO(2) from [1-(14)C] or [6-(14)C]D-glucose...
  54. ncbi Coenzyme specificity of enzymes in the oxidative pentose phosphate pathway of Gluconobacter oxydans
    Naoto Tonouchi
    AminoScience Laboratories, Ajinomoto Co, Inc, Kawasaki-shi, Japan
    Biosci Biotechnol Biochem 67:2648-51. 2003
    The coenzyme specificity of enzymes in the oxidative pentose phosphate pathway of Gluconobacter oxydans was investigated...
  55. ncbi Global metabolic response of Escherichia coli to gnd or zwf gene-knockout, based on 13C-labeling experiments and the measurement of enzyme activities
    J Zhao
    Metabolome Unit, Institute for Advanced Biosciences, Keio University, Tsuruoka, Japan
    Appl Microbiol Biotechnol 64:91-8. 2004
    ..how the central metabolism of Escherichia coli changes with the knockout of genes in the oxidative pentose phosphate pathway (PPP)...
  56. ncbi [Kinetic parameters of androgen receptor complexes and the activities of the glycolysis and oxidative pentose phosphate pathway key enzymes in rat testis cytosol after whole body 60-min exposure to high frequency electromagnetic field (39.5 Ghz)]
    E F Kopoplia
    Institute of Radiobiology, Belarus National Academs of Sciences, Minsk, 220141 Belarus
    Radiats Biol Radioecol 43:535-7. 2003
    ..68-fold reduced in relation to the control; through 1-month after exposure the rising of [RA] (123%) was registered; however, to 3-month term the significant fall (1.42 times) of the RA contents in testicular cytosol persisted...
  57. ncbi Biosynthesis of camptothecin. In silico and in vivo tracer study from [1-13C]glucose
    Yasuyo Yamazaki
    Graduate School of Pharmaceutical Sciences, Chiba University, 1-33 Yayoi-cho, Inage-ku, Chiba 263-8522, Japan
    Plant Physiol 134:161-70. 2004
    ..This study provides the innovative example for how a computer-aided comprehensive metabolic analysis will refine the experimental design to obtain more precise biological information...
  58. ncbi Evidences of high carbon catabolic enzyme activities during sporulation of Pleurotus ostreatus (Florida)
    Tapas K Chakraborty
    Indian Institute of Chemical Biology, 4 Raja S. C. Mullick Road, Kolkata--700 032, India
    J Basic Microbiol 43:462-7. 2003
    ..Enzymes of the pentose phosphate pathway always showed lower specific activities as compared to those of the enzymes of the glycolytic pathway...
  59. ncbi Metabolic strategy of boar spermatozoa revealed by a metabolomic characterization
    Silvia Marin
    Department of Biochemistry and Molecular Biology, University of Barcelona, , Barcelona 08028, Spain
    FEBS Lett 554:342-6. 2003
    ..In contrast to the known metabolic activities in dog sperm, boar spermatozoa have low levels of energy production and biosynthetic activities suggesting two different metabolic profiles for the two different phenotypes...
  60. ncbi Metabolic analysis of acetate accumulation during xylose consumption by Paenibacillus polymyxa
    B Marwoto
    Department of Molecular Biotechnology, Hiroshima University, Graduate School of Advanced Sciences of Matter, 1-3-1, 739-8530 Kagamiyama, Higashi-Hiroshima, Japan
    Appl Microbiol Biotechnol 64:112-9. 2004
    ..In fed-batch culture on xylose, glucose feeding raised the butanediol yield to 0.56 mol/mol sugar and reduced acetate accumulation to 0.04 mol/mol sugar...
  61. ncbi Analysis of Escherichia coli anaplerotic metabolism and its regulation mechanisms from the metabolic responses to altered dilution rates and phosphoenolpyruvate carboxykinase knockout
    Chen Yang
    Metabolome Unit, Institute for Advanced Biosciences, Keio University, Tsuruoka 997 0017, Japan
    Biotechnol Bioeng 84:129-44. 2003
    ..Combining the measurement data of in vivo fluxes, metabolite concentrations and enzyme activities, the in vivo regulations of PEP carboxykinase flux, PEP carboxylation, and glyoxylate shunt in E. coli are discussed...
  62. ncbi Stress responses in alfalfa (Medicago sativa L.) XIX. Transcriptional activation of oxidative pentose phosphate pathway genes at the onset of the isoflavonoid phytoalexin response
    T Fahrendorf
    Plant Biology Division, Samuel Roberts Noble Foundation, Ardmore, OK 73401, USA
    Plant Mol Biol 28:885-900. 1995
    We have isolated cDNA clones encoding the pentose phosphate pathway enzymes 6-phosphogluconate dehydrogenase (6PGDH, EC 1.1.1.44) and glucose 6-phosphate dehydrogenase (G6PDH, EC 1.1.1.49) from alfalfa (Medicago sativa L.)...