protein binding

Summary

Summary: The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.

Top Publications

  1. pmc JASPAR 2010: the greatly expanded open-access database of transcription factor binding profiles
    Elodie Portales-Casamar
    Department of Medical Genetics, Centre for Molecular Medicine and Therapeutics, Child and Family Research Institute, University of British Columbia, 950 West 28th Avenue, Vancouver, BC V5Z 4H4, Canada
    Nucleic Acids Res 38:D105-10. 2010
  2. pmc Identification and analysis of functional elements in 1% of the human genome by the ENCODE pilot project
    Ewan Birney
    Nature 447:799-816. 2007
  3. pmc Combinatorial patterns of histone acetylations and methylations in the human genome
    Zhibin Wang
    Laboratory of Molecular Immunology, National Heart, Lung, and Blood Institute, US National Institutes of Health, Bethesda, Maryland 20892, USA
    Nat Genet 40:897-903. 2008
  4. ncbi p62/SQSTM1 binds directly to Atg8/LC3 to facilitate degradation of ubiquitinated protein aggregates by autophagy
    Serhiy Pankiv
    Biochemistry Department, Institute of Medical Biology, University of Tromsø, 9037 Tromsø, Norway
    J Biol Chem 282:24131-45. 2007
  5. pmc AutoDock4 and AutoDockTools4: Automated docking with selective receptor flexibility
    Garrett M Morris
    Department of Molecular Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    J Comput Chem 30:2785-91. 2009
  6. pmc Structures of the CXCR4 chemokine GPCR with small-molecule and cyclic peptide antagonists
    Beili Wu
    Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Science 330:1066-71. 2010
  7. pmc Crystal structure of the β2 adrenergic receptor-Gs protein complex
    Søren G F Rasmussen
    Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, California 94305, USA
    Nature 477:549-55. 2011
  8. pmc p62/SQSTM1 forms protein aggregates degraded by autophagy and has a protective effect on huntingtin-induced cell death
    Geir Bjørkøy
    Biochemistry Department, Institute of Medical Biology, University of Tromsø, 9037 Tromsø, Norway
    J Cell Biol 171:603-14. 2005
  9. doi Breaking the code of DNA binding specificity of TAL-type III effectors
    Jens Boch
    Department of Genetics, Institute of Biology, Martin Luther University Halle Wittenberg, Weinbergweg 10, D 06099 Halle Saale Germany
    Science 326:1509-12. 2009
  10. ncbi Crystal structure of the nucleosome core particle at 2.8 A resolution
    K Luger
    Institut fur Molekularbiologie und Biophysik, Zurich, Switzerland
    Nature 389:251-60. 1997

Research Grants

  1. Molecular mechanism of antiangiogenic properties of gold nanoparticle
    Priyabrata Mukherjee; Fiscal Year: 2010
  2. PAIRING OF HOMOLOGOUS SEQUENCES IN DROSOPHILA
    KENT GOLIC; Fiscal Year: 2002
  3. Ty3 viruslike particle morphogenesis and host interactions
    Suzanne Sandmeyer; Fiscal Year: 2010
  4. CHEMISTRY AND BIOLOGY OF PLATINUM ANTICANCER DRUGS
    STEPHEN LIPPARD; Fiscal Year: 2007
  5. CELL CYCLE REGULATION OF THE YEAST HO GENE
    LINDA BREEDEN; Fiscal Year: 2001
  6. SMOOTH MUSCLE MYOSIN PHOSPHATASE SUBUNIT ISOFORMS
    Steven Fisher; Fiscal Year: 2007
  7. PROTEIN BINDING/ORGAN PERFUSION AND RENAL DRUG TRANSPORT
    David Smith; Fiscal Year: 1990
  8. MODIFICATION OF ALPHA-CRYSTALLIN CHAPERONE FUNCTION
    EDATHARA ABRAHAM; Fiscal Year: 2003
  9. Vaccinia Proteome Affinity Reagents From Phage Display
    Philip Felgner; Fiscal Year: 2005
  10. ELECTROCHEMICAL DNA-BASED SENSORS
    Jacqueline K Barton; Fiscal Year: 2010

Detail Information

Publications300 found, 100 shown here

  1. pmc JASPAR 2010: the greatly expanded open-access database of transcription factor binding profiles
    Elodie Portales-Casamar
    Department of Medical Genetics, Centre for Molecular Medicine and Therapeutics, Child and Family Research Institute, University of British Columbia, 950 West 28th Avenue, Vancouver, BC V5Z 4H4, Canada
    Nucleic Acids Res 38:D105-10. 2010
    ..Additionally, three new special collections provide matrix profile data produced by recent alternative high-throughput approaches...
  2. pmc Identification and analysis of functional elements in 1% of the human genome by the ENCODE pilot project
    Ewan Birney
    Nature 447:799-816. 2007
    ..Together, these studies are defining a path for pursuit of a more comprehensive characterization of human genome function...
  3. pmc Combinatorial patterns of histone acetylations and methylations in the human genome
    Zhibin Wang
    Laboratory of Molecular Immunology, National Heart, Lung, and Blood Institute, US National Institutes of Health, Bethesda, Maryland 20892, USA
    Nat Genet 40:897-903. 2008
    ..Our data suggest that these histone modifications may act cooperatively to prepare chromatin for transcriptional activation...
  4. ncbi p62/SQSTM1 binds directly to Atg8/LC3 to facilitate degradation of ubiquitinated protein aggregates by autophagy
    Serhiy Pankiv
    Biochemistry Department, Institute of Medical Biology, University of Tromsø, 9037 Tromsø, Norway
    J Biol Chem 282:24131-45. 2007
    ..In fact, p62 bodies and these structures are indistinguishable. Taken together, our results clearly suggest that p62 is required both for the formation and the degradation of polyubiquitin-containing bodies by autophagy...
  5. pmc AutoDock4 and AutoDockTools4: Automated docking with selective receptor flexibility
    Garrett M Morris
    Department of Molecular Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    J Comput Chem 30:2785-91. 2009
    ..We also report its utility in analysis of covalently bound ligands, using both a grid-based docking method and a modification of the flexible sidechain technique...
  6. pmc Structures of the CXCR4 chemokine GPCR with small-molecule and cyclic peptide antagonists
    Beili Wu
    Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Science 330:1066-71. 2010
    ..These structures provide new clues about the interactions between CXCR4 and its natural ligand CXCL12, and with the HIV-1 glycoprotein gp120...
  7. pmc Crystal structure of the β2 adrenergic receptor-Gs protein complex
    Søren G F Rasmussen
    Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, California 94305, USA
    Nature 477:549-55. 2011
    ..The most surprising observation is a major displacement of the α-helical domain of Gαs relative to the Ras-like GTPase domain. This crystal structure represents the first high-resolution view of transmembrane signalling by a GPCR...
  8. pmc p62/SQSTM1 forms protein aggregates degraded by autophagy and has a protective effect on huntingtin-induced cell death
    Geir Bjørkøy
    Biochemistry Department, Institute of Medical Biology, University of Tromsø, 9037 Tromsø, Norway
    J Cell Biol 171:603-14. 2005
    ..We suggest that p62 may, via LC3, be involved in linking polyubiquitinated protein aggregates to the autophagy machinery...
  9. doi Breaking the code of DNA binding specificity of TAL-type III effectors
    Jens Boch
    Department of Genetics, Institute of Biology, Martin Luther University Halle Wittenberg, Weinbergweg 10, D 06099 Halle Saale Germany
    Science 326:1509-12. 2009
    ..Our study describes the functionality of a distinct type of DNA binding domain and allows the design of DNA binding domains for biotechnology...
  10. ncbi Crystal structure of the nucleosome core particle at 2.8 A resolution
    K Luger
    Institut fur Molekularbiologie und Biophysik, Zurich, Switzerland
    Nature 389:251-60. 1997
    ..The lack of uniformity between multiple histone/DNA-binding sites causes the DNA to deviate from ideal superhelix geometry...
  11. ncbi hSIR2(SIRT1) functions as an NAD-dependent p53 deacetylase
    H Vaziri
    Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA
    Cell 107:149-59. 2001
    ..In contrast, expression of a catalytically inactive hSir2 protein potentiates p53-dependent apoptosis and radiosensitivity. We propose that hSir2 is involved in the regulation of p53 function via deacetylation...
  12. pmc An oestrogen-receptor-alpha-bound human chromatin interactome
    Melissa J Fullwood
    Genome Institute of Singapore, Agency for Science, Technology and Research, Singapore 138672
    Nature 462:58-64. 2009
    ..We propose that chromatin interactions constitute a primary mechanism for regulating transcription in mammalian genomes...
  13. pmc Five-vertebrate ChIP-seq reveals the evolutionary dynamics of transcription factor binding
    Dominic Schmidt
    Cancer Research UK, Cambridge Research Institute, Li Ka Shing Centre, Robinson Way, Cambridge CB2 0RE, UK
    Science 328:1036-40. 2010
    ..Our results reveal large interspecies differences in transcriptional regulation and provide insight into regulatory evolution...
  14. ncbi The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinases
    J W Harper
    Verna and Marrs McLean Department of Biochemistry, Baylor College of Medicine, Houston, Texas 77030
    Cell 75:805-16. 1993
    ..Cotransfection experiments indicate that CIP1 and SV40 T antigen function in a mutually antagonistic manner to control cell cycle progression...
  15. ncbi Mdm2 promotes the rapid degradation of p53
    Y Haupt
    Lautenberg Center for General and Tumor Immunology, The Hebrew University Haddassah Medical School, Jerusalem, Israel
    Nature 387:296-9. 1997
    ..During recovery from DNA damage, maximal Mdm2 induction coincides with rapid p53 loss. We propose that the Mdm2-promoted degradation of p53 provides a new mechanism to ensure effective termination of the p53 signal...
  16. pmc Core transcriptional regulatory circuitry in human embryonic stem cells
    Laurie A Boyer
    Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, Massachusetts 02142, USA
    Cell 122:947-56. 2005
    ..These results provide new insights into the transcriptional regulation of stem cells and reveal how OCT4, SOX2, and NANOG contribute to pluripotency and self-renewal...
  17. ncbi Genome-wide mapping of in vivo protein-DNA interactions
    David S Johnson
    Department of Genetics, Stanford University School of Medicine, Stanford, CA, 94305 5120, USA
    Science 316:1497-502. 2007
    ..96] and statistical confidence (P <10(-4)), properties that were important for inferring new candidate interactions. These include key transcription factors in the gene network that regulates pancreatic islet cell development...
  18. doi Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis
    Jesper V Olsen
    Department of Proteomics and Signal Transduction, Max Planck Institute for Biochemistry, Am Klopferspitz 18, D 82152 Martinsried near Munich, Germany
    Sci Signal 3:ra3. 2010
    ..In particular, nuclear proteins and proteins involved in regulating metabolic processes have high phosphorylation site occupancy in mitosis. This suggests that these proteins may be inactivated by phosphorylation in mitotic cells...
  19. pmc Abscisic acid inhibits type 2C protein phosphatases via the PYR/PYL family of START proteins
    Sang Youl Park
    Department of Botany and Plant Sciences, University of California at Riverside, Riverside, CA 92521, USA
    Science 324:1068-71. 2009
    ..Our results illustrate the power of the chemical genetic approach for sidestepping genetic redundancy...
  20. ncbi Cardif is an adaptor protein in the RIG-I antiviral pathway and is targeted by hepatitis C virus
    Etienne Meylan
    Department of Biochemistry, University of Lausanne, BIL Biomedical Research Center, Chemin des Boveresses 155, CH 1066 Epalinges, Switzerland
    Nature 437:1167-72. 2005
    ..Cardif thus functions as an adaptor, linking the cytoplasmic dsRNA receptor RIG-I to the initiation of antiviral programmes...
  21. pmc The 2.6 angstrom crystal structure of a human A2A adenosine receptor bound to an antagonist
    Veli Pekka Jaakola
    Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037 USA
    Science 322:1211-7. 2008
    ....
  22. doi A quantitative analysis of kinase inhibitor selectivity
    Mazen W Karaman
    Ambit Biosciences, 4215 Sorrento Valley Blvd, San Diego, California 92121, USA
    Nat Biotechnol 26:127-32. 2008
    ..We further investigate the impact of panel size and find that small assay panels do not provide a robust measure of selectivity...
  23. ncbi GEF means go: turning on RHO GTPases with guanine nucleotide-exchange factors
    Kent L Rossman
    Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Nat Rev Mol Cell Biol 6:167-80. 2005
    ..The failure to do so can have significant consequences and is reflected in the aberrant function of Dbl-family GEFs in some human diseases...
  24. ncbi HDAC6 is a microtubule-associated deacetylase
    Charlotte Hubbert
    Department of Pharmacology and Cancer Biology, Duke University, Durham, North Carolina 27710, USA
    Nature 417:455-8. 2002
    ..Our results show that HDAC6 is the tubulin deacetylase, and provide evidence that reversible acetylation regulates important biological processes beyond histone metabolism and gene transcription...
  25. pmc Structure of P-glycoprotein reveals a molecular basis for poly-specific drug binding
    Stephen G Aller
    Department of Molecular Biology, Scripps Research Institute, 10550 North Torrey Pines Road, CB105, La Jolla, CA 92037, USA
    Science 323:1718-22. 2009
    ..The inward-facing conformation represents an initial stage of the transport cycle that is competent for drug binding...
  26. ncbi DNA damage activates ATM through intermolecular autophosphorylation and dimer dissociation
    Christopher J Bakkenist
    Department of Hematology Oncology, St Jude Children s Research Hospital, 332 North Lauderdale Street, Memphis, Tennessee 38105, USA
    Nature 421:499-506. 2003
    ....
  27. doi Chromatin accessibility pre-determines glucocorticoid receptor binding patterns
    Sam John
    Laboratory of Receptor Biology and Gene Expression, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA
    Nat Genet 43:264-8. 2011
    ..The results define a framework for understanding regulatory factor-genome interactions and provide a molecular basis for the tissue selectivity of steroid pharmaceuticals and other agents that intersect the living genome...
  28. doi Crystal structure of the eukaryotic ribosome
    Adam Ben-Shem
    IGBMC Institut de Génétique et de Biologie Moléculaire et Cellulaire, 1 rue Laurent Fries, BP10142, Illkirch F 67400, France
    Science 330:1203-9. 2010
    ..We describe the conformational changes in both ribosomal subunits that are involved in ratcheting and their implications in coordination between the two associated subunits and in mRNA and tRNA translocation...
  29. ncbi Sensing DNA damage through ATRIP recognition of RPA-ssDNA complexes
    Lee Zou
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Science 300:1542-8. 2003
    ..Our data suggest that RPA-coated ssDNA is the critical structure at sites of DNA damage that recruits the ATR-ATRIP complex and facilitates its recognition of substrates for phosphorylation and the initiation of checkpoint signaling...
  30. pmc Regulation of alternative splicing by histone modifications
    Reini F Luco
    National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Science 327:996-1000. 2010
    ..Histone marks affect splicing outcome by influencing the recruitment of splicing regulators via a chromatin-binding protein. These results outline an adaptor system for the reading of histone marks by the pre-mRNA splicing machinery...
  31. pmc Cellular prion protein mediates impairment of synaptic plasticity by amyloid-beta oligomers
    Juha Lauren
    Cellular Neuroscience, Neurodegeneration and Repair Program, Yale University School of Medicine, New Haven, Connecticut 06536, USA
    Nature 457:1128-32. 2009
    ..Thus, PrP(C) is a mediator of amyloid-beta-oligomer-induced synaptic dysfunction, and PrP(C)-specific pharmaceuticals may have therapeutic potential for Alzheimer's disease...
  32. ncbi Negative control of p53 by Sir2alpha promotes cell survival under stress
    J Luo
    Institute of Cancer Genetics and Department of Pathology, College of Physicians and Surgeons, Columbia University, 1150 St Nicholas Avenue, New York, NY 10032, USA
    Cell 107:137-48. 2001
    ..These results have significant implications regarding an important role for Sir2alpha in modulating the sensitivity of cells in p53-dependent apoptotic response and the possible effect in cancer therapy...
  33. ncbi Global analysis of protein localization in budding yeast
    Won Ki Huh
    Howard Hughes Medical Institute, University of California San Francisco, Department of Biochemistry and Biophysics, 600 16th Street, San Francisco, California 94143 2240, USA
    Nature 425:686-91. 2003
    ....
  34. pmc An empirical framework for binary interactome mapping
    Kavitha Venkatesan
    Center for Cancer Systems Biology and Department of Cancer Biology, Dana Farber Cancer Institute, 1 Jimmy Fund Way, Boston, MA 02115, USA
    Nat Methods 6:83-90. 2009
    ....
  35. ncbi Dynamic interaction of BiP and ER stress transducers in the unfolded-protein response
    A Bertolotti
    Skirball Institute of Biomolecular Medicine, Departments of Medicine and Cell Biology and the Kaplan Cancer Center, New York University School of Medicine, New York, New York 10016, USA
    Nat Cell Biol 2:326-32. 2000
    ..These findings are consistent with a model in which BiP represses signalling through PERK and IRE1 and protein misfolding relieves this repression by effecting the release of BiP from the PERK and IRE1 lumenal domains...
  36. pmc APP binds DR6 to trigger axon pruning and neuron death via distinct caspases
    Anatoly Nikolaev
    Division of Research, Genentech, Inc, 1 DNA Way, South San Francisco, California 94080, USA
    Nature 457:981-9. 2009
    ..Our results indicate that APP and DR6 are components of a neuronal self-destruction pathway, and suggest that an extracellular fragment of APP, acting via DR6 and caspase 6, contributes to Alzheimer's disease...
  37. pmc Structure of the human histamine H1 receptor complex with doxepin
    Tatsuro Shimamura
    Human Receptor Crystallography Project, ERATO, Japan Science and Technology Agency, Kyoto 606 8501, Japan
    Nature 475:65-70. 2011
    ..Our study sheds light on the molecular basis of H(1)R antagonist specificity against H(1)R...
  38. pmc Membrane fusion: grappling with SNARE and SM proteins
    Thomas C Sudhof
    Department of Cellular and Molecular Physiology, Stanford University, Palo Alto, CA 94304, USA
    Science 323:474-7. 2009
    ....
  39. pmc High-resolution DNA-binding specificity analysis of yeast transcription factors
    Cong Zhu
    Division of Genetics, Department of Medicine, Brigham and Women s Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA
    Genome Res 19:556-66. 2009
    ..These approaches could be adapted to identify TFs and cis regulatory elements in higher eukaryotes...
  40. pmc The KEGG resource for deciphering the genome
    Minoru Kanehisa
    Bioinformatics Center, Institute for Chemical Research, Kyoto University, Uji, Kyoto 611 0011, Japan
    Nucleic Acids Res 32:D277-80. 2004
    ..New efforts are being made to abstract knowledge, both computationally and manually, about ortholog clusters in the KO (KEGG Orthology) database, and to collect and analyze carbohydrate structures in the GLYCAN database...
  41. pmc Glycogen synthase kinase-3beta regulates cyclin D1 proteolysis and subcellular localization
    J A Diehl
    Howard Hughes Medical Institute, St Jude Children s Research Hospital, Memphis, Tennessee 38105 USA
    Genes Dev 12:3499-511. 1998
    ..Therefore, phosphorylation and proteolytic turnover of cyclin D1 and its subcellular localization during the cell division cycle are linked through the action of GSK-3beta...
  42. doi The structural basis of lipopolysaccharide recognition by the TLR4-MD-2 complex
    Beom Seok Park
    Department of Chemistry, KAIST, Daejeon, 305 701, Korea
    Nature 458:1191-5. 2009
    ..The TLR4-MD-2-LPS structure illustrates the remarkable versatility of the ligand recognition mechanisms employed by the TLR family, which is essential for defence against diverse microbial infection...
  43. pmc Design and analysis of ChIP-seq experiments for DNA-binding proteins
    Peter V Kharchenko
    Center for Biomedical Informatics, Harvard Medical School, 10 Shattuck St, Boston, Massachusetts 02115, USA
    Nat Biotechnol 26:1351-9. 2008
    ..We also analyze the relationship between the depth of sequencing and characteristics of the detected binding positions, and provide a method for estimating the sequencing depth necessary for a desired coverage of protein binding sites.
  44. pmc Diversity and complexity in DNA recognition by transcription factors
    Gwenael Badis
    Banting and Best Department of Medical Research, University of Toronto, Toronto, ON M5S 3E1, Canada
    Science 324:1720-3. 2009
    ..This complexity in DNA recognition may be important in gene regulation and in the evolution of transcriptional regulatory networks...
  45. pmc Structural basis for RNA 3'-end recognition by Hfq
    Evelyn Sauer
    Department of Biochemistry, Max Planck Institute for Developmental Biology, 72076 Tubingen, Germany
    Proc Natl Acad Sci U S A 108:13065-70. 2011
    ..The capacity of Hfq to occupy and sequester the RNA 3' end has important implications for the mechanisms by which Hfq is thought to affect sRNA stability, turnover, and regulation...
  46. ncbi Transcriptional repression by the methyl-CpG-binding protein MeCP2 involves a histone deacetylase complex
    X Nan
    Institute of Cell and Molecular Biology, University of Edinburgh, UK
    Nature 393:386-9. 1998
    ..The data suggest that two global mechanisms of gene regulation, DNA methylation and histone deacetylation, can be linked by MeCP2...
  47. ncbi Specificity and stability in topology of protein networks
    Sergei Maslov
    Department of Physics, Brookhaven National Laboratory, Upton, NY 11973, USA
    Science 296:910-3. 2002
    ..This effect decreases the likelihood of cross talk between different functional modules of the cell and increases the overall robustness of a network by localizing effects of deleterious perturbations...
  48. doi Transposable elements have rewired the core regulatory network of human embryonic stem cells
    Galih Kunarso
    Computational and Mathematical Biology, Genome Institute of Singapore, Singapore, Singapore
    Nat Genet 42:631-4. 2010
    ..These data indicate that species-specific transposable elements have substantially altered the transcriptional circuitry of pluripotent stem cells...
  49. pmc Induced fit, conformational selection and independent dynamic segments: an extended view of binding events
    Peter Csermely
    Department of Medical Chemistry, Semmelweis University, PO Box 260, H 1444 Budapest 8, Hungary
    Trends Biochem Sci 35:539-46. 2010
    ..Additionally, we highlight the dynamic complexity of binding scenarios as they relate to events such as aggregation and signalling, and the crowded cellular environment...
  50. ncbi Drosophila enhancer of Zeste/ESC complexes have a histone H3 methyltransferase activity that marks chromosomal Polycomb sites
    Birgit Czermin
    Adolf Butenandt Institut, University of Munich, Schillerstrasse 44, 80336 Munich, Germany
    Cell 111:185-96. 2002
    ..Histone H3 methylated in vitro by the E(Z)/ESC complex binds specifically to Polycomb protein...
  51. ncbi Refined structure of alpha beta-tubulin at 3.5 A resolution
    J Lowe
    MRC Laboratory of Molecular Biology, Cambridge, UK
    J Mol Biol 313:1045-57. 2001
    ..In light of the new model we discuss details of the tubulin structure such as nucleotide and taxol binding sites, lateral contacts in zinc-sheets, and the significance of the location of highly conserved residues...
  52. ncbi Genome-wide profiles of STAT1 DNA association using chromatin immunoprecipitation and massively parallel sequencing
    Gordon Robertson
    British Columbia Cancer Agency Genome Sciences Centre, 675 West 10th Avenue, Vancouver, British Columbia V5Z 4S6, Canada
    Nat Methods 4:651-7. 2007
    ..ChIP-seq targets were enriched in sequences similar to known STAT1 binding motifs. Comparisons with two ChIP-PCR data sets suggested that ChIP-seq sensitivity was between 70% and 92% and specificity was at least 95%...
  53. ncbi p53 has a direct apoptogenic role at the mitochondria
    Motohiro Mihara
    Department of Pathology, Stony Brook University, Stony Brook, NY 11794, USA
    Mol Cell 11:577-90. 2003
    ..This opens the possibility that mutations might represent "double-hits" by abrogating the transcriptional and mitochondrial apoptotic activity of p53...
  54. ncbi The tumour suppressor protein VHL targets hypoxia-inducible factors for oxygen-dependent proteolysis
    P H Maxwell
    Wellcome Trust Centre for Human Genetics, Oxford, UK
    Nature 399:271-5. 1999
    ..Thus, constitutive HIF-1 activation may underlie the angiogenic phenotype of VHL-associated tumours. The pVHL/HIF-1 interaction provides a new focus for understanding cellular oxygen sensing...
  55. pmc The Rag GTPases bind raptor and mediate amino acid signaling to mTORC1
    Yasemin Sancak
    Whitehead Institute for Biomedical Research and Department of Biology, Massachusetts Institute of Technology MIT, Nine Cambridge Center, Cambridge, MA 02142, USA
    Science 320:1496-501. 2008
    ..The Rag proteins do not directly stimulate the kinase activity of mTORC1, but, like amino acids, promote the intracellular localization of mTOR to a compartment that also contains its activator Rheb...
  56. pmc Robust hepatitis C virus infection in vitro
    Jin Zhong
    Departments of Molecular and Experimental Medicine and Immunology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 102:9294-9. 2005
    ..This system provides a powerful tool for the analysis of host-virus interactions that should facilitate the discovery of antiviral drugs and vaccines for this important human pathogen...
  57. doi The tail of integrins, talin, and kindlins
    Markus Moser
    Max Planck Institute of Biochemistry, 82152 Martinsried, Germany
    Science 324:895-9. 2009
    ..This review focuses on the mechanisms whereby two key proteins, talin and the kindlins, regulate integrin activation by binding the tails of integrin-beta subunits...
  58. pmc Structure of a V3-containing HIV-1 gp120 core
    Chih Chin Huang
    Vaccine Research Center, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892, USA
    Science 310:1025-8. 2005
    ..The extended nature and antibody accessibility of V3 explain its immunodominance. Together, the results provide a structural rationale for the role of V3 in HIV entry and neutralization...
  59. ncbi The Microprocessor complex mediates the genesis of microRNAs
    Richard I Gregory
    The Wistar Institute, 3601 Spruce Street, Philadelphia, Pennsylvania 19104, USA
    Nature 432:235-40. 2004
    ..In vivo knock-down and in vitro reconstitution studies revealed that both components of this smaller complex, termed Microprocessor, are necessary and sufficient in mediating the genesis of miRNAs from the primary miRNA transcript...
  60. pmc Diverse RNA-binding proteins interact with functionally related sets of RNAs, suggesting an extensive regulatory system
    Daniel J Hogan
    Department of Biochemistry, Stanford University School of Medicine, Stanford, California, United States of America
    PLoS Biol 6:e255. 2008
    ..These results strongly suggest that combinatorial binding of RBPs to specific recognition elements in mRNAs is a pervasive mechanism for multi-dimensional regulation of their post-transcriptional fate...
  61. pmc Inactivation of YAP oncoprotein by the Hippo pathway is involved in cell contact inhibition and tissue growth control
    Bin Zhao
    Life Sciences Institute, University of Michigan, Ann Arbor, Michigan 48109, USA
    Genes Dev 21:2747-61. 2007
    ..Our observations demonstrate that YAP plays a key role in the Hippo pathway to control cell proliferation in response to cell contact...
  62. ncbi MatInspector and beyond: promoter analysis based on transcription factor binding sites
    K Cartharius
    Genomatix Software GmbH Landsberger Strasse 6, 80339 München, Germany
    Bioinformatics 21:2933-42. 2005
    ..The next steps in promoter analysis can be tackled only with reliable predictions, e.g. finding phylogenetically conserved patterns or identifying higher order combinations of sites in promoters of co-regulated genes...
  63. doi Ubiquitin-binding domains - from structures to functions
    Ivan Dikic
    Institute of Biochemistry II and Cluster of Excellence Macromolecular Complexes, Goethe University Frankfurt, Germany
    Nat Rev Mol Cell Biol 10:659-71. 2009
    ..These new structure-based insights provide strategies for controlling cellular processes by targeting ubiquitin-UBD interfaces...
  64. pmc Preferential associations between co-regulated genes reveal a transcriptional interactome in erythroid cells
    Stefan Schoenfelder
    Laboratory of Chromatin and Gene Expression, Babraham Research Campus, Cambridge, UK
    Nat Genet 42:53-61. 2010
    ..Our results establish a new gene expression paradigm, implying that active co-regulated genes and their regulatory factors cooperate to create specialized nuclear hot spots optimized for efficient and coordinated transcriptional control...
  65. ncbi Functional interaction of beta-catenin with the transcription factor LEF-1
    J Behrens
    Max Delbruck Centre for Molecular Medicine, Berlin, Germany
    Nature 382:638-42. 1996
    ..Thus beta-catenin regulates gene expression by direct interaction with transcription factors such as LEF-1, providing a molecular mechanism for the transmission of signals, from cell-adhesion components or wnt protein to the nucleus...
  66. ncbi Mass spectrometry-based proteomics
    Ruedi Aebersold
    Institute for Systems Biology, 1441 North 34th Street, Seattle, Washington 98103 8904, USA
    Nature 422:198-207. 2003
    ..The ability of mass spectrometry to identify and, increasingly, to precisely quantify thousands of proteins from complex samples can be expected to impact broadly on biology and medicine...
  67. pmc Purified human BRCA2 stimulates RAD51-mediated recombination
    Ryan B Jensen
    Department of Microbiology, University of California, Davis, California 95616, USA
    Nature 467:678-83. 2010
    ....
  68. doi Converging concepts of protein folding in vitro and in vivo
    F Ulrich Hartl
    Max Planck Institute of Biochemistry, Department of Cellular Biochemistry, Martinsried, Germany
    Nat Struct Mol Biol 16:574-81. 2009
    ....
  69. pmc Recognition of trimethylated histone H3 lysine 4 facilitates the recruitment of transcription postinitiation factors and pre-mRNA splicing
    Robert J Sims
    Department of Biochemistry, New York University School of Medicine, New York, NY 10016, USA
    Mol Cell 28:665-76. 2007
    ..These findings suggest that methylated H3K4 serves to facilitate the competency of pre-mRNA maturation through the bridging of spliceosomal components to H3K4me3 via CHD1...
  70. pmc Developmental roles of 21 Drosophila transcription factors are determined by quantitative differences in binding to an overlapping set of thousands of genomic regions
    Stewart MacArthur
    Genomics Division, Lawrence Berkeley National Laboratory, Cyclotron Road MS 84 181, Berkeley, CA 94720, USA
    Genome Biol 10:R80. 2009
    ....
  71. pmc Systematic analysis of human protein complexes identifies chromosome segregation proteins
    James R A Hutchins
    Research Institute of Molecular Pathology IMP, Dr Bohr Gasse 7, A 1030 Vienna, Austria
    Science 328:593-9. 2010
    ..The approaches we describe here are generally applicable to high-throughput follow-up analyses of phenotypic screens in mammalian cells...
  72. ncbi SIR2 and SIR4 interactions differ in core and extended telomeric heterochromatin in yeast
    S Strahl-Bolsinger
    Department of Biological Chemistry, University of California, Los Angeles 90095, USA
    Genes Dev 11:83-93. 1997
    ..RAP1 binding at the core region is unaffected by SIR3 overproduction and RAP1 shows no evidence of spreading. Thus, we propose that the structure of core telomeric heterochromatin differs from that extended by SIR3...
  73. ncbi A semiempirical free energy force field with charge-based desolvation
    Ruth Huey
    Department of Molecular Biology, Scripps Research Institute, La Jolla, California 92102, USA
    J Comput Chem 28:1145-52. 2007
    ..The force field shows improvement in redocking simulations over the previous AutoDock3 force field...
  74. pmc Genome-wide analysis of ETS-family DNA-binding in vitro and in vivo
    Gong Hong Wei
    Public Health Genomics Unit, National Institute for Health and Welfare THL and Genome Scale Biology Program, Institute of Biomedicine and High Throughput Center, University of Helsinki, Biomedicum, Helsinki, Finland
    EMBO J 29:2147-60. 2010
    ..The results indicate that even relatively small differences in in vitro binding specificity of a TF contribute to site selectivity in vivo...
  75. pmc Benchmarking sets for molecular docking
    Niu Huang
    Department of Pharmaceutical Chemistry, University of California San Francisco, QB3 Building, 1700 4th Street, Box 2550, San Francisco, California 94143 2550, USA
    J Med Chem 49:6789-801. 2006
    ..DUD is freely available online as a benchmarking set for docking at http://blaster.docking.org/dud/...
  76. ncbi Global, in vivo, and site-specific phosphorylation dynamics in signaling networks
    Jesper V Olsen
    Center for Experimental Bioinformatics, Department of Biochemistry and Molecular Biology, University of Southern Denmark, DK 5230 Odense, Denmark
    Cell 127:635-48. 2006
    ..The dynamic phosphoproteome provides a missing link in a global, integrative view of cellular regulation...
  77. pmc Molecular architecture of native HIV-1 gp120 trimers
    Jun Liu
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland 20892, USA
    Nature 455:109-13. 2008
    ..Our findings elucidate the structure and conformational changes of trimeric HIV-1 gp120 relevant to antibody neutralization and attachment to target cells...
  78. ncbi The transcription factor snail controls epithelial-mesenchymal transitions by repressing E-cadherin expression
    A Cano
    Instituto de Investigaciones Biomedicas, Arturo Duperier 4, 28029 Madrid, Spain
    Nat Cell Biol 2:76-83. 2000
    ..Snail may thus be considered as a marker for malignancy, opening up new avenues for the design of specific anti-invasive drugs...
  79. doi MeCP2 binding to DNA depends upon hydration at methyl-CpG
    Kok Lian Ho
    School of Biological Sciences, University of Edinburgh, King s Buildings, Mayfield Road, Edinburgh EH9 3JR, UK
    Mol Cell 29:525-31. 2008
    ..The MeCP2-DNA complex also identifies a unique structural role for T158, the residue most commonly mutated in Rett syndrome...
  80. ncbi Visualization of protein interactions in living plant cells using bimolecular fluorescence complementation
    Michael Walter
    Institut fur Botanik und Botanischer Garten, Molekulare Entwicklungsbiologie der Pflanzen, Universitat Munster, Schlossplatz 4, 48149 Munster, Germany
    Plant J 40:428-38. 2004
    ..Consequently, the BiFC approach should significantly facilitate the visualization of the subcellular sites of protein interactions under conditions that closely reflect the normal physiological environment...
  81. pmc A dual-kinase mechanism for Wnt co-receptor phosphorylation and activation
    Xin Zeng
    Neurobiology Program, Children s Hospital Boston, Department of Neurology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 438:873-7. 2005
    ....
  82. pmc Frequent gain and loss of functional transcription factor binding sites
    Scott W Doniger
    Computational Biology Program, Washington University School of Medicine, St Louis, Missouri, United States of America
    PLoS Comput Biol 3:e99. 2007
    ..The frequent gain and loss of TFBSs implies that cis-regulatory sequences are labile and, in the absence of turnover, may contribute to species-specific patterns of gene expression...
  83. ncbi A flagellin-induced complex of the receptor FLS2 and BAK1 initiates plant defence
    Delphine Chinchilla
    Zurich Basel Plant Science Center, Botanical Institute, University of Basel, Hebelstrasse 1, 4056 Basel, Switzerland
    Nature 448:497-500. 2007
    ..Thus, BAK1 is not only associated with developmental regulation through the plant hormone receptor BRI1 (refs 6,7), but also has a functional role in PRR-dependent signalling, which initiates innate immunity...
  84. ncbi MDM2 inhibitors for cancer therapy
    Lyubomir T Vassilev
    Discovery Oncology, Roche Research Center, Hoffmann La Roche Inc, Nutley, NJ 07110, USA
    Trends Mol Med 13:23-31. 2007
    ..Here, the new developments in the quest for pharmacological p53 activators are reviewed with an emphasis on small-molecule inhibitors of the p53-MDM2 interaction...
  85. pmc Neurexins induce differentiation of GABA and glutamate postsynaptic specializations via neuroligins
    Ethan R Graf
    Department of Anatomy and Neurobiology, Washington University School of Medicine, 660 S Euclid Avenue, St Louis, MO 63110, USA
    Cell 119:1013-26. 2004
    ....
  86. pmc Antidepressant specificity of serotonin transporter suggested by three LeuT-SSRI structures
    Zheng Zhou
    Kimmel Center for Biology and Medicine at the Skirball Institute of Biomolecular Medicine, Department of Cell Biology, New York University School of Medicine, New York, New York, USA
    Nat Struct Mol Biol 16:652-7. 2009
    ..Thus, the specificity of SERT for SSRIs is dependent largely on interaction of the drug halogens with the protein's HBP...
  87. pmc Predicting protein ligand binding sites by combining evolutionary sequence conservation and 3D structure
    John A Capra
    Department of Computer Science, Princeton University, Princeton, New Jersey, United States of America
    PLoS Comput Biol 5:e1000585. 2009
    ..Data, source code, and prediction visualizations are available on the ConCavity web site (http://compbio.cs.princeton.edu/concavity/)...
  88. ncbi Nutrient control of glucose homeostasis through a complex of PGC-1alpha and SIRT1
    Joseph T Rodgers
    Department of Cell Biology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Nature 434:113-8. 2005
    ..Thus, we have identified a molecular mechanism whereby SIRT1 functions in glucose homeostasis as a modulator of PGC-1alpha. These findings have strong implications for the basic pathways of energy homeostasis, diabetes and lifespan...
  89. doi Cracking the RNA polymerase II CTD code
    Sylvain Egloff
    Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, UK
    Trends Genet 24:280-8. 2008
    ..How and when is the code written and read? How does it contribute to transcription and coordinate RNA processing?..
  90. pmc Cyclic di-GMP allosterically inhibits the CRP-like protein (Clp) of Xanthomonas axonopodis pv. citri
    Jason L Leduc
    Department of Bacteriology, 1550 Linden Drive, University of Wisconsin Madison, Madison, WI 53706, USA
    J Bacteriol 191:7121-2. 2009
    ..We show that unlike the DNA-binding activity of other members of this family, the DNA-binding activity of Clp is allosterically inhibited by its effector and that cyclic di-GMP serves as that effector at physiological concentrations...
  91. pmc Structural basis for recognition of H3K4 methylation status by the DNA methyltransferase 3A ATRX-DNMT3-DNMT3L domain
    Junji Otani
    Department of Molecular Engineering, Graduate School of Engineering, Kyoto University, Kyoto Daigaku Katsura, Nishikyo ku, Kyoto 615 8510, Japan
    EMBO Rep 10:1235-41. 2009
    ..These results indicate that de novo DNA methylation by DNMT3A requires the alteration of chromatin structure...
  92. pmc The mammalian clock component PERIOD2 coordinates circadian output by interaction with nuclear receptors
    Isabelle Schmutz
    Department of Medicine, Unit of Biochemistry, University of Fribourg, 1700 Fribourg, Switzerland
    Genes Dev 24:345-57. 2010
    ..The concept that PER2 may propagate clock information to metabolic pathways via nuclear receptors adds an important facet to the clock-dependent regulation of biological networks...
  93. pmc Structure of Escherichia coli Hfq bound to polyriboadenylate RNA
    Todd M Link
    Department of Biochemistry and Molecular Biology, University of Texas, MD Anderson Cancer Center, Houston, TX 77030 4009, USA
    Proc Natl Acad Sci U S A 106:19292-7. 2009
    ..The abundance of (A-R-N)(4) and (A-R-N)(5) triplet repeats in the E. coli genome suggests additional RNA targets for Hfq. Further, the structure provides insight into Hfq-mediated sRNA-mRNA annealing and the role of Hfq in RNA decay...
  94. ncbi p53AIP1, a potential mediator of p53-dependent apoptosis, and its regulation by Ser-46-phosphorylated p53
    K Oda
    Laboratory of Molecular Medicine, Human Genome Center, Institute of Medical Science, University of Tokyo, Japan
    Cell 102:849-62. 2000
    ..Our results suggest that p53AIP1 is likely to play an important role in mediating p53-dependent apoptosis, and phosphorylation of Ser-46 regulates the transcriptional activation of this apoptosis-inducing gene...
  95. pmc In-frame deletion in a novel centrosomal/ciliary protein CEP290/NPHP6 perturbs its interaction with RPGR and results in early-onset retinal degeneration in the rd16 mouse
    Bo Chang
    The Jackson Laboratory, Bar Harbor, ME 04609, USA
    Hum Mol Genet 15:1847-57. 2006
    ..Our findings suggest a critical function for CEP290 in ciliary transport and provide insights into the mechanism of early-onset photoreceptor degeneration...
  96. ncbi Curvature of clathrin-coated pits driven by epsin
    Marijn G J Ford
    MRC Laboratory of Molecular Biology, Cambridge, UK
    Nature 419:361-6. 2002
    ..We propose that this helix is inserted into one leaflet of the lipid bilayer, inducing curvature. On lipid monolayers epsin alone is sufficient to facilitate the formation of clathrin-coated invaginations...
  97. pmc A comprehensive map of insulator elements for the Drosophila genome
    Nicolas Negre
    Institute for Genomics and Systems Biology, Department of Human Genetics, and Department of Ecology and Evolution, University of Chicago, Chicago, Illinois, USA
    PLoS Genet 6:e1000814. 2010
    ..Together, these results provide a map demarcating the boundaries of gene regulatory units and a framework for understanding insulator function during the development and evolution of Drosophila...
  98. doi The pharmacology of mTOR inhibition
    David A Guertin
    Whitehead Institute for Biomedical Research, Nine Cambridge Center, Cambridge, MA 02141, USA
    Sci Signal 2:pe24. 2009
    ..Here, we summarize exciting findings regarding mTOR signaling and the outlook for mTOR inhibitors as tools to study the mTOR pathway and as drugs in the clinic...
  99. pmc Dynamic strength of molecular adhesion bonds
    E Evans
    Department of Physics, University of British Columbia, Vancouver, Canada
    Biophys J 72:1541-55. 1997
    ..Balsera, Y. Oono, and K. Schulten. 1997. Molecular dynamics study of unbinding of the avidin-biotin complex. Biophys. J., this issue), we describe how Brownian dynamics can help bridge the gap between molecular dynamics and probe tests...
  100. pmc Circadian clock feedback cycle through NAMPT-mediated NAD+ biosynthesis
    Kathryn Moynihan Ramsey
    Department of Medicine, Northwestern University Feinberg School of Medicine, 2200 Campus Drive, Evanston, IL 60208 3500, USA
    Science 324:651-4. 2009
    ..In turn, the circadian transcription factor CLOCK binds to and up-regulates Nampt, thus completing a feedback loop involving NAMPT/NAD+ and SIRT1/CLOCK:BMAL1...
  101. ncbi Improved protein-ligand docking using GOLD
    Marcel L Verdonk
    Astex Technology, Ltd, Cambridge, United Kingdom
    Proteins 52:609-23. 2003
    ..Even at docking speeds of around 1-2 min/compound, the Goldscore function predicts binding energies with a standard deviation of approximately 10.5 kJ/mol...

Research Grants74

  1. Molecular mechanism of antiangiogenic properties of gold nanoparticle
    Priyabrata Mukherjee; Fiscal Year: 2010
    ..Determine, in detail, pharmacological properties of gold nanoparticles, biodistribution, toxicity and plasma protein binding properties of AuNPs, and 2) Delineate the molecular mechanism of anti-angiogenic properties of AuNP in vivo...
  2. PAIRING OF HOMOLOGOUS SEQUENCES IN DROSOPHILA
    KENT GOLIC; Fiscal Year: 2002
    ..These candidates include the PRE sequences recognized by Polycomb Group proteins, polymers of the Zeste protein binding site, repeats of the AG dinucleotide recognized by the GAGA protein (the product of the Trithorax-like gene), ..
  3. Ty3 viruslike particle morphogenesis and host interactions
    Suzanne Sandmeyer; Fiscal Year: 2010
    ..SELEX and in vitro RNA-protein binding assays will be used to identify important nucleocapsid binding contacts in the packaging site of the Ty3 UTR ..
  4. CHEMISTRY AND BIOLOGY OF PLATINUM ANTICANCER DRUGS
    STEPHEN LIPPARD; Fiscal Year: 2007
    ..b) To investigate the kinetics and thermodynamics of HMG-domain protein binding to cisplatin-modified DNA...
  5. CELL CYCLE REGULATION OF THE YEAST HO GENE
    LINDA BREEDEN; Fiscal Year: 2001
    ..Most of the research proposal is an investigation of the proteins and protein binding sites required for these two waves of transcriptional activation...
  6. SMOOTH MUSCLE MYOSIN PHOSPHATASE SUBUNIT ISOFORMS
    Steven Fisher; Fiscal Year: 2007
    ..We used mutation/deletion of MYPT1 mini-gene constructs, gain-and-loss of function, and RNA-protein binding experiments to show 1) TIA and SR protein binding to regulatory elements near the 5' splice site are ..
  7. PROTEIN BINDING/ORGAN PERFUSION AND RENAL DRUG TRANSPORT
    David Smith; Fiscal Year: 1990
    ..In particular, the effect of protein binding and renal blood flow on the secretory transport of drug is still considered in a descriptive rather than ..
  8. MODIFICATION OF ALPHA-CRYSTALLIN CHAPERONE FUNCTION
    EDATHARA ABRAHAM; Fiscal Year: 2003
    ..in vivo and that can be inflicted in vitro will be correlated with chaperone activity and chaperone-target protein binding. The specific aims of this proposal are based on our most recent findings that glycation, oxidation and mixed ..
  9. Vaccinia Proteome Affinity Reagents From Phage Display
    Philip Felgner; Fiscal Year: 2005
    ..Weiss (Co-Investigator), will be used to hone binding affinity and specificity of vaccinia protein binding partners...
  10. ELECTROCHEMICAL DNA-BASED SENSORS
    Jacqueline K Barton; Fiscal Year: 2010
    ..base pairs and hence can be used as a sensor for changes in base stacking that arise with base mismatches or protein binding. Electrical sensors for mutation detection and for base flipping proteins that bind to DNA have been ..
  11. Cellular Retinoic Acid Binding Proteins: Functional Studies
    Lorraine Gudas; Fiscal Year: 2009
    ..and 3 null cells; and deletion of DNA regulatory elements such as the Hoxa1 RARE, followed by analysis of PcG protein binding. We also propose a comparison of 3T3 cells with H-Ras transformed 3T3 cells to assess how an oncogenic ..
  12. Cellular Retinoic Acid Binding Proteins: Functional Studies
    Lorraine J Gudas; Fiscal Year: 2010
    ..and 3 null cells;and deletion of DNA regulatory elements such as the Hoxa1 RARE, followed by analysis of PcG protein binding. We also propose a comparison of 3T3 cells with H-Ras transformed 3T3 cells to assess how an oncogenic ..
  13. ACYL GLUCURONIDES: COVALENT BINDING & PHARMACOKINETICS
    Leslie Benet; Fiscal Year: 1993
    ..aims of this grant proposal are to determine the scope and biological significance of the rearrangement and protein binding reactions of acyl glucuronides, and to establish whether irreversible binding of these metabolites to ..
  14. Analysis of the Human c-myc Gene Replication Origin
    Michael Leffak; Fiscal Year: 2010
    ..identified the replication origin 5'to the human c-myc oncogene, and we have shown that the structure, protein binding and function of the core c-myc replicator as a replication origin are the same at its endogenous chromosomal ..
  15. Analysis of the Human c-myc Gene Replication Origin
    Michael Leffak; Fiscal Year: 2009
    ..identified the replication origin 5' to the human c-myc oncogene, and we have shown that the structure, protein binding and function of the core c-myc replicator as a replication origin are the same at its endogenous chromosomal ..
  16. Novel Micro-X-ray Translucent Flow Cell Technology for Proteomics, a Broadly Appl
    EVA BIRNBAUM; Fiscal Year: 2007
    ..has the potential to provide benefit to all biomedical and pharmaceutical research which involves drug to protein binding. The proposed project is a method and apparatus for rapidly characterizing proteins for their binding ..
  17. Molecular Principles of FGF-HSGAG Interactions
    Ram Sasisekharan; Fiscal Year: 2006
    ..Our preliminary studies point out to exciting properties associated with HSGAG upon protein binding. While the overall helical structure is maintained in the protein-bound HSGAGs, a "kink" in the helical axis ..
  18. Transgenic Targeting of Vascular G Protein Signaling
    Andrea Eckhart; Fiscal Year: 2009
    ..GRK-phosphorylated 7TMRs are inhibited from association with their normal heterotrimeric G protein binding partner via arrestin binding...
  19. Transgenic Targeting of Vascular G Protein Signaling
    Karsten Peppel; Fiscal Year: 2010
    ..GRK-phosphorylated 7TMRs are inhibited from association with their normal heterotrimeric G protein binding partner via arrestin binding...
  20. Membrane Structure and Enzyme Activity
    HOWARD BROCKMAN; Fiscal Year: 2005
    ..understand how interactions between phospholipids and non-phospholipid second messengers regulate peripheral protein binding to interfaces and the subsequent expression of catalytic activity...
  21. MEMBRANE FUSION IN RETINAL ROD OUTER SEGMENTS
    Kathleen Boesze Battaglia; Fiscal Year: 2009
    ..of p/rds in OS morphogenesis and stability and understand the physiological implications of binary (direct) protein binding to this domain...