competitive binding

Summary

Top Publications

  1. ncbi Co-operative regulation of ligand binding to melanocortin receptor subtypes: evidence for interacting binding sites
    Sergei Kopanchuk
    Department of Pharmaceutical Biosciences, Uppsala University, BMC Box 591, 751 24 Uppsala, Sweden
    Eur J Pharmacol 512:85-95. 2005
  2. ncbi Nicotinic acetylcholine receptors on basophils and mast cells
    P S Sudheer
    Department of Anaesthetics and Intensive Care Medicine, University Hospital of Wales, Cardiff, UK
    Anaesthesia 61:1170-4. 2006
  3. doi Recombinant transthyretin purification and competitive binding with organohalogen compounds in two gull species (Larus argentatus and Larus hyperboreus)
    Francisco Ucán-Marin
    National Wildlife Research Centre, Carleton University, Ottawa, Ontario, K1A 0H3, Canada
    Toxicol Sci 107:440-50. 2009
  4. doi Competitive binding of poly- and perfluorinated compounds to the thyroid hormone transport protein transthyretin
    Jana M Weiss
    Institute for Environmental Studies, Department of Chemistry and Biology, VU University, 1081 HV Amsterdam, The Netherlands
    Toxicol Sci 109:206-16. 2009
  5. pmc PUMA promotes Bax translocation by both directly interacting with Bax and by competitive binding to Bcl-X L during UV-induced apoptosis
    Yingjie Zhang
    Ministry of Education Key Laboratory of Laser Life Science and Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China
    Mol Biol Cell 20:3077-87. 2009
  6. pmc Polyamines regulate the stability of JunD mRNA by modulating the competitive binding of its 3' untranslated region to HuR and AUF1
    Tongtong Zou
    Department of Surgery, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
    Mol Cell Biol 30:5021-32. 2010
  7. pmc Local conformations and competitive binding affinities of single- and double-stranded primer-template DNA at the polymerization and editing active sites of DNA polymerases
    Kausiki Datta
    Institute of Molecular Biology and Department of Chemistry, University of Oregon, Eugene, Oregon 97403 1229, USA
    J Biol Chem 284:17180-93. 2009
  8. ncbi Stimulation of aromatase P450 promoter (II) activity in endometriosis and its inhibition in endometrium are regulated by competitive binding of steroidogenic factor-1 and chicken ovalbumin upstream promoter transcription factor to the same cis-acting elem
    K Zeitoun
    Cecil H Ida Green Center for Reproductive Biology Sciences, Department of Obstetrics and Gynecology, The University of Texas Southwestern Medical Center, Dallas 75235 9051, USA
    Mol Endocrinol 13:239-53. 1999
  9. ncbi Avidity regulation of integrins: the driving force in leukocyte adhesion
    Y van Kooyk
    Department of Tumor Immunology, University Medical Center Nijmegen, St Radboud, P Van Leydenlaan 25, 6525 EX, The, Nijmegen, Netherlands
    Curr Opin Cell Biol 12:542-7. 2000
  10. ncbi New multifunctional molecular conjugate vector for targeting, imaging, and therapy of tumors
    Elisabeth Garanger
    Groupe de Recherche sur le Cancer du Poumon, INSERM U578, Institut Albert Bonniot, La Tronche 38706 Cedex, France
    Mol Ther 12:1168-75. 2005

Detail Information

Publications227 found, 100 shown here

  1. ncbi Co-operative regulation of ligand binding to melanocortin receptor subtypes: evidence for interacting binding sites
    Sergei Kopanchuk
    Department of Pharmaceutical Biosciences, Uppsala University, BMC Box 591, 751 24 Uppsala, Sweden
    Eur J Pharmacol 512:85-95. 2005
    ..The data indicate that the binding of MSH peptides to melanocortin receptors are governed by a complex dynamic homotropic co-operative regulations...
  2. ncbi Nicotinic acetylcholine receptors on basophils and mast cells
    P S Sudheer
    Department of Anaesthetics and Intensive Care Medicine, University Hospital of Wales, Cardiff, UK
    Anaesthesia 61:1170-4. 2006
    ..The identity of this receptor was confirmed by reverse transcriptase PCR and quantitative PCR...
  3. doi Recombinant transthyretin purification and competitive binding with organohalogen compounds in two gull species (Larus argentatus and Larus hyperboreus)
    Francisco Ucán-Marin
    National Wildlife Research Centre, Carleton University, Ottawa, Ontario, K1A 0H3, Canada
    Toxicol Sci 107:440-50. 2009
    ..the OH-substituted analogs all had lower K(i) and K(d) values, indicating greater affinity and more potent competitive binding to both T(3) and T(4)...
  4. doi Competitive binding of poly- and perfluorinated compounds to the thyroid hormone transport protein transthyretin
    Jana M Weiss
    Institute for Environmental Studies, Department of Chemistry and Biology, VU University, 1081 HV Amsterdam, The Netherlands
    Toxicol Sci 109:206-16. 2009
    ..b>Competitive binding of PFCs to TTR, as observed for human TTR in the present study, may explain altered thyroid hormone levels ..
  5. pmc PUMA promotes Bax translocation by both directly interacting with Bax and by competitive binding to Bcl-X L during UV-induced apoptosis
    Yingjie Zhang
    Ministry of Education Key Laboratory of Laser Life Science and Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China
    Mol Biol Cell 20:3077-87. 2009
    ..Together, these studies create an important conclusion that PUMA promotes Bax translocation by both by directly interacting with Bax and by competitive binding to Bcl-X(L) in UV-induced apoptosis.
  6. pmc Polyamines regulate the stability of JunD mRNA by modulating the competitive binding of its 3' untranslated region to HuR and AUF1
    Tongtong Zou
    Department of Surgery, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
    Mol Cell Biol 30:5021-32. 2010
    ..JunD mRNA is a target of HuR and AUF1 and that polyamines modulate JunD mRNA degradation by altering the competitive binding of HuR and AUF1 to the JunD 3'-UTR...
  7. pmc Local conformations and competitive binding affinities of single- and double-stranded primer-template DNA at the polymerization and editing active sites of DNA polymerases
    Kausiki Datta
    Institute of Molecular Biology and Department of Chemistry, University of Oregon, Eugene, Oregon 97403 1229, USA
    J Biol Chem 284:17180-93. 2009
    ..These distributions and the conformational changes in the P/T DNA that occur during template-directed DNA synthesis in solution illuminate some of the mechanisms used by DNA polymerases to assure the fidelity of DNA synthesis...
  8. ncbi Stimulation of aromatase P450 promoter (II) activity in endometriosis and its inhibition in endometrium are regulated by competitive binding of steroidogenic factor-1 and chicken ovalbumin upstream promoter transcription factor to the same cis-acting elem
    K Zeitoun
    Cecil H Ida Green Center for Reproductive Biology Sciences, Department of Obstetrics and Gynecology, The University of Texas Southwestern Medical Center, Dallas 75235 9051, USA
    Mol Endocrinol 13:239-53. 1999
    ....
  9. ncbi Avidity regulation of integrins: the driving force in leukocyte adhesion
    Y van Kooyk
    Department of Tumor Immunology, University Medical Center Nijmegen, St Radboud, P Van Leydenlaan 25, 6525 EX, The, Nijmegen, Netherlands
    Curr Opin Cell Biol 12:542-7. 2000
    ....
  10. ncbi New multifunctional molecular conjugate vector for targeting, imaging, and therapy of tumors
    Elisabeth Garanger
    Groupe de Recherche sur le Cancer du Poumon, INSERM U578, Institut Albert Bonniot, La Tronche 38706 Cedex, France
    Mol Ther 12:1168-75. 2005
    ....
  11. ncbi Rat liver and kidney contain high densities of sigma 1 and sigma 2 receptors: characterization by ligand binding and photoaffinity labeling
    S B Hellewell
    Unit on Receptor Biochemistry and Pharmacology, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, MD 20892
    Eur J Pharmacol 268:9-18. 1994
    ..These data are consistent with the notion that the 25 kDa and 21.5 kDa proteins represent sigma 1 and sigma 2 receptors, respectively.(ABSTRACT TRUNCATED AT 400 WORDS)..
  12. ncbi A 26 S protease subunit that binds ubiquitin conjugates
    Q Deveraux
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City 84132
    J Biol Chem 269:7059-61. 1994
    ..Thus, proteins conjugated to polymers of ubiquitin may be selected for degradation by a single subunit of the 26 S protease complex...
  13. ncbi The steroid-responsive hiccup reflex arc: competitive binding to the corticosteroid-receptor?
    Rob D Dickerman
    Department of Neurosurgery, North Shore University Hospital, New Hyde Park, NY, USA
    Neuro Endocrinol Lett 24:167-9. 2003
    ..This report validates the previous report on anabolic steroids inducing hiccups and exemplifies the ability for steroids as a class, due to there backbone structural homology, to induce function even as competitive inhibitors...
  14. doi Opposing effects of Ndel1 and alpha1 or alpha2 on cytoplasmic dynein through competitive binding to Lis1
    Chong Ding
    Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China
    J Cell Sci 122:2820-7. 2009
    ..They also help to explain why brain development is particularly sensitive to a decrease in Lis1 levels...
  15. ncbi Possible existence of common internalization mechanisms among arginine-rich peptides
    Tomoki Suzuki
    Institute for Chemical Research, Kyoto University, Uji, Kyoto 611 0011, Japan
    J Biol Chem 277:2437-43. 2002
    ..These results strongly suggest that the arginine-rich basic peptides share a certain part of the internalization pathway...
  16. ncbi Molecular cloning, functional expression, and signaling characteristics of a C-C chemokine receptor
    K Neote
    Department of Immunology, Genentech, Incorporated, South San Francisco, California 94080
    Cell 72:415-25. 1993
    ..Finally, C-C chemokines were shown to bind a C-C CKR-1-related gene product encoded by cytomegalovirus, suggesting a role for C-C chemokines in viral immunity...
  17. pmc Elucidating the relationship between DISC1, NDEL1 and NDE1 and the risk for schizophrenia: evidence of epistasis and competitive binding
    Katherine E Burdick
    Department of Psychiatry Research, The Zucker Hillside Hospital, North Shore Long Island Jewish Health System, Glen Oaks, NY 11004, USA
    Hum Mol Genet 17:2462-73. 2008
    ..These data suggest that NDEL1 significantly influences risk for SZ via an interaction with DISC1. We propose a model where NDEL1 and NDE1 compete for binding with DISC1...
  18. ncbi Changes in conformation and subcellular distribution of alpha4beta2 nicotinic acetylcholine receptors revealed by chronic nicotine treatment and expression of subunit chimeras
    Patricia C Harkness
    Department of Pharmacology, University College London, London, WC1E 6BT, United Kingdom
    J Neurosci 22:10172-81. 2002
    ..The finding that subunit conformation can be influenced by coassembled subunit partners is in agreement with models of receptor assembly which propose that subunit folding continues after initial subunit-subunit interactions...
  19. ncbi Protection against anthrax toxin by recombinant antibody fragments correlates with antigen affinity
    Jennifer A Maynard
    Department of Chemical Engineering, University of Texas, Austin, TX 78712, USA
    Nat Biotechnol 20:597-601. 2002
    ..High-affinity toxin-neutralizing antibodies may be of therapeutic value for alleviating the symptoms of anthrax toxin in infected individuals and for medium-term prophylaxis to infection...
  20. ncbi Syntaxin 5 is a common component of the NSF- and p97-mediated reassembly pathways of Golgi cisternae from mitotic Golgi fragments in vitro
    C Rabouille
    Cell Biology Laboratory, Imperial Cancer Research Fund, London, United Kingdom
    Cell 92:603-10. 1998
    ..p47 also mediates the binding of p97 to syntaxin 5 and so plays an analogous role to alpha-SNAP, which mediates the binding of NSF...
  21. ncbi The Sp1-like protein BTEB3 inhibits transcription via the basic transcription element box by interacting with mSin3A and HDAC-1 co-repressors and competing with Sp1
    J Kaczynski
    Gastroenterology Research Unit, Mayo Clinic, Rochester, Minnesota 55901, USA
    J Biol Chem 276:36749-56. 2001
    ..Furthermore, our results suggest a mechanism of repression for BTEB3 involving direct repression by the N terminus via interaction with mSin3A and HDAC-1 and competition with Sp1 via the DNA-binding domain...
  22. pmc Collaborative competition mechanism for gene activation in vivo
    Joanna A Miller
    Department of Biochemistry, Molecular Biology and Cellular Biology, Northwestern University, Evanston, Illinois 60208 3500, USA
    Mol Cell Biol 23:1623-32. 2003
    ..We speculate that initial target site recognition and binding may occur via spontaneous nucleosomal site exposure, with remodeling factor action required downstream to lock in higher levels of regulatory protein occupancy...
  23. ncbi Molecular cloning, pharmacological characterization, and brain mapping of the melanocortin 4 receptor in the goldfish: involvement in the control of food intake
    Jose Miguel Cerdá-Reverter
    Department of Biological Sciences, University of Alberta, Edmonton, Alberta, T6G 2E9 Canada
    Endocrinology 144:2336-49. 2003
    ....
  24. pmc Dual interactions of the translational repressor Paip2 with poly(A) binding protein
    K Khaleghpour
    Department of Biochemistry and McGill Cancer Center, McGill University, Montreal, Quebec, Canada H3G 1Y6
    Mol Cell Biol 21:5200-13. 2001
    ..Significantly, only the central Paip2 fragment, which binds with high affinity to the PABP RRM region, inhibits PABP binding to poly(A) RNA and translation...
  25. ncbi Potent competitive interactions of some brominated flame retardants and related compounds with human transthyretin in vitro
    I A Meerts
    Toxicology Group, Department of Food Technology and Nutritional Sciences, Wageningen University and Research Center, Tuinlaan 5, 6703 HE Wageningen, The Netherlands
    Toxicol Sci 56:95-104. 2000
    ..the possible interaction of several brominated flame retardants with T(4) binding to TTR in an in vitro competitive binding assay, using human TTR and 125 I-T(4) as the displaceable radioligand...
  26. ncbi CD4-induced interaction of primary HIV-1 gp120 glycoproteins with the chemokine receptor CCR-5
    L Wu
    LeukoSite, Inc, Cambridge, Massachusetts 02142, USA
    Nature 384:179-83. 1996
    ..These results suggest that HIV-1 attachment to CD4 creates a high-affinity binding site for CCR-5, leading to membrane fusion and virus entry...
  27. ncbi RAC-3 is a NF-kappa B coactivator
    S Werbajh
    Deparmento de Cs Biológicas, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellon II, Argentina
    FEBS Lett 485:195-9. 2000
    ..The competition between GR and NF-kappa B for binding to RAC3 may represent a general mechanism by which both transcription factors mutually antagonize their activity...
  28. ncbi Function of sigma1 receptors in Parkinson's disease
    M Mishina
    The Department of Neurology, Neurological Institute, Nippon Medical School Chiba Hokusoh Hospital, Inba gun, Chiba, Japan
    Acta Neurol Scand 112:103-7. 2005
    ....
  29. ncbi The breast cell growth inhibitor, estrogen down regulated gene 1, modulates a novel functional interaction between estrogen receptor alpha and transcriptional elongation factor cyclin T1
    Bryan M Wittmann
    Department of Pharmacology, Case Western Reserve University, Cleveland, OH 44106, USA
    Oncogene 24:5576-88. 2005
    ..Conversely, increased EDG1 expression results in inhibition of cyclin T1 recruitment and ERalpha DNA binding. Our results support a novel functional interaction between ERalpha and cyclin T1 that is modulated by EDG1...
  30. pmc Affinity and location of an internal K+ ion binding site in shaker K channels
    J Thompson
    Department of Pharmacology and Physiology, University of Rochester Medical Center, Rochester, New York 14642, USA
    J Gen Physiol 117:373-84. 2001
    ..These results lead to a refined picture of the number and location of ion binding sites at the inner end of the pore in Shaker K channels...
  31. ncbi Biotransformation of brominated flame retardants into potentially endocrine-disrupting metabolites, with special attention to 2,2',4,4'-tetrabromodiphenyl ether (BDE-47)
    Timo Hamers
    Institute for Environmental Studies IVM, Vrije Universiteit Amsterdam, Amsterdam, The Netherlands
    Mol Nutr Food Res 52:284-98. 2008
    ..In combination with other environmentally relevant hydroxylated organohalogens acting on TTR-binding and E2SULT inhibition, internal exposure to BFR metabolites may significantly contribute to the overall risk of endocrine disruption...
  32. ncbi Poly(ADP-ribose) polymerase-1 is a negative regulator of HIV-1 transcription through competitive binding to TAR RNA with Tat.positive transcription elongation factor b (p-TEFb) complex
    Marianne Parent
    Division of Health and Environmental Research, Laval University Medical Center, Ste Foy, Quebec G1V 4G2, Canada
    J Biol Chem 280:448-57. 2005
    ..Thus, these results suggest that PARP-1 acts as a negative regulator of HIV-1 transcription through competitive binding with Tat or the Tat.P-TEFb complex to TAR RNA.
  33. ncbi Proteasome subunit Rpn1 binds ubiquitin-like protein domains
    Suzanne Elsasser
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Nat Cell Biol 4:725-30. 2002
    ..We also report that, contrary to expectation, the base subunit Rpn10 does not mediate the binding of UBL proteins to the proteasome in yeast, although it can apparently contribute to the binding of ubiquitin chains by intact proteasomes...
  34. ncbi Competitive binding of ibuprofen and naproxen to bovine serum albumin : modified form of drug-drug displacement interaction at the binding site
    M M Rahman
    Pharmacy Discipline, Life Science School, Khulna University, Khulna 9208, Bangladesh
    Pak J Pharm Sci 18:43-7. 2005
    The competitive binding characteristics of ibuprofen and naproxen with respect to binding site on bovine serum albumin (BSA) was studied by equilibrium dialysis method at pH 7.4 and 25 degrees C...
  35. pmc Competitive binding inhibition enzyme-linked immunosorbent assay that uses the secreted aspartyl proteinase of Candida albicans as an antigenic marker for diagnosis of disseminated candidiasis
    Christine J Morrison
    Mycotic Diseases Branch, Division of Bacterial and Mycotic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    Clin Diagn Lab Immunol 10:835-48. 2003
    ..The potential use of proteinases as markers of invasive candidiasis led us to develop a competitive binding inhibition enzyme-linked immunosorbent assay (ELISA) to detect Sap in clinical specimens...
  36. ncbi Mechanism of Cd2+ toxicity: Cd2+ inhibits photoactivation of Photosystem II by competitive binding to the essential Ca2+ site
    Peter Faller
    Institut fur Biologie II, Biochemie der Pflanzen, Universitat Freiburg, Schänzlestr 1, 79104 Freiburg, Germany
    Biochim Biophys Acta 1706:158-64. 2005
    ..This mechanism is likely to be important for Cd2+ toxicity towards photosynthetic organisms in general, at least in unicellular like C. reinhardtii where Cd2+ has easy access to the photosynthetic apparatus...
  37. doi Fab antibodies capable of blocking T cells by competitive binding have the identical specificity but a higher affinity to the MHC-peptide-complex than the T cell receptor
    Frank Neumann
    I Med Klinik, Saarland University Medical School, 66421 Homburg Saar, Germany
    Immunol Lett 125:86-92. 2009
    ..These results indicate that molecules that block T cells by competitive binding with the TCR must have the same specificity but higher affinity for the MHC-peptide-complex than the TCR.
  38. doi Competitive binding of musclin to natriuretic peptide receptor 3 with atrial natriuretic peptide
    Shunbun Kita
    Pharmaceutical Research Division, Pharmacology Research Laboratories I, Takeda Pharmaceutical Company Ltd, Yodogawa ku, Osaka 532 8686, Japan
    J Endocrinol 201:287-95. 2009
    ..We conclude that musclin binds to NPR3 competitively with ANP and may affect ANP concentrations in a local or systemic manner...
  39. ncbi ULBP1, 2, 3: novel MHC class I-related molecules that bind to human cytomegalovirus glycoprotein UL16, activate NK cells
    M Kubin
    Department of Immunobiology, Immunex Corporation, Seattle 98101, USA
    Eur J Immunol 31:1428-37. 2001
    ..Addition of soluble recombinant UL16 protein inhibited the biological activities mediated by ULBP, suggesting the existence of a novel mechanism utilized by CMV to evade elimination by the host immune system...
  40. doi Competitive binding exchange between alkali metal ions (K+, Rb+, and Cs+) and Na+ ions bound to the dimeric quadruplex [d(G4T4G4)]2: a 23Na and 1H NMR study
    Flaminia Cesare Marincola
    Dipartimento di Scienze Chimiche, Universita di Cagliari, Cittadella Universitaria di Monserrato, S S 554 Bivio per Sestu, I 09042, Monserrato, Cagliari, Italy
    Magn Reson Chem 47:1036-42. 2009
    ....
  41. pmc Streptococcus gordonii Hsa environmentally constrains competitive binding by Streptococcus sanguinis to saliva-coated hydroxyapatite
    Angela H Nobbs
    Department of Diagnostic and Biological Sciences, University of Minnesota, 17 164 Moos Tower, 515 Delaware Street, S E, Minneapolis, MN 55455, USA
    J Bacteriol 189:3106-14. 2007
    ..Next, putative S. gordonii adhesins were analyzed for contributions to interspecies competitive binding. Like wild-type S...
  42. doi Electrochemical survey of the chain length influence in phytochelatins competitive binding by cadmium
    Rui Gusmão
    Departament de Quimica Analitica, Universitat de Barcelona, Spain
    Anal Biochem 406:61-9. 2010
    ..with alternating least squares (MCR-ALS) was applied to voltammetric data obtained in the analysis of the competitive binding of glutathione (GSH) and phytochelatins [(gammaGlu-Cys)(n)-Gly, PC(n), n=2-5] by Cd(2+)...
  43. ncbi Nicotine upregulates its own receptors through enhanced intracellular maturation
    Jérôme Sallette
    CNRS URA D2182 Récepteurs et Cognition, Institut Pasteur, Paris, France
    Neuron 46:595-607. 2005
    ....
  44. ncbi Competitive binding of postsynaptic density 95 and Ca2+-calmodulin dependent protein kinase II to N-methyl-D-aspartate receptor subunit 2B in rat brain
    Fan Jie Meng
    Research Center of Biochemistry and Molecular Biology, Xuzhou Medical College, Xuzhou 221002, China
    Acta Pharmacol Sin 25:176-80. 2004
    ....
  45. ncbi Cloning and functional expression of the human histamine H3 receptor
    T W Lovenberg
    R W Johnson Pharmaceutical Research Institute, San Diego, California, USA
    Mol Pharmacol 55:1101-7. 1999
    ..The availability of the human H3 receptor cDNA should greatly aid in the development of chemical and biological reagents, allowing a greater appreciation of the role of histamine in brain function...
  46. ncbi Pharmacological comparison of rat and human melanocortin 3 and 4 receptors in vitro
    Helgi B Schioth
    Department of Neuroscience, Biomedical Center, Box 593, 751 24 Uppsala University, Uppsala, Sweden
    Regul Pept 106:7-12. 2002
    ..Pharmacological species difference have earlier been reported for the MC1 and MC5 receptors but this is the first report showing important differences between the rat and human MC4 receptors...
  47. ncbi Inhibition of the transcription of CYP1A1 gene by the upstream stimulatory factor 1 in rabbits. Competitive binding of USF1 with AhR.Arnt complex
    Y Takahashi
    Division of Drug Metabolism, Faculty of Pharmaceutical Sciences, Hokkaido University, N12W6, Kita ku, Sapporo, Hokkaido 060, Japan
    J Biol Chem 272:30025-31. 1997
    ..These results indicate that the XRE of the rabbit CYP1A1 gene is recognized by the basic helix-loop-helix proteins to regulate the expression of CYP1A1 in both an agonistic (AhR.Arnt) and an antagonistic (USF1) manner...
  48. ncbi Structure-function analysis of a phage display-derived peptide that binds to insulin-like growth factor binding protein 1
    N J Skelton
    Department of Protein Engineering, Genentech, Inc, 1 DNA Way, South San Francisco, California 94080, USA
    Biochemistry 40:8487-98. 2001
    ..By comparison of alanine scanning data for IGF-I and the turn-helix peptide, we propose a model for common surface features of these molecules that recognize IGFBP-1...
  49. pmc The E-SCREEN assay as a tool to identify estrogens: an update on estrogenic environmental pollutants
    A M Soto
    Tufts University School of Medicine, Department of Anatomy and Cellular Biology, Boston, MA 02111, USA
    Environ Health Perspect 103:113-22. 1995
    ....
  50. ncbi Lysine 58 and histidine 66 at the C-terminal alpha-helix of monocyte chemoattractant protein-1 are essential for glycosaminoglycan binding
    L Chakravarty
    Neurobiotechnology Center and the Departments of Biochemistry and Medical Biochemistry, The Ohio State University, Columbus, Ohio 43210, USA
    J Biol Chem 273:29641-7. 1998
    ..Therefore, we conclude that the Lys-58 and His-66 residues in the C-terminal alpha-helix of MCP-1 are essential for glycosaminoglycan binding and probably for the binding to the endothelial surface proteoglycans...
  51. ncbi Intron-mediated expression of the human neuropeptide Y Y1 receptor
    Ulrica Marklund
    Department of Molecular Biology, AstraZeneca R and D, Tvistevägen 48, S 907 36 Umeå, Sweden
    Mol Cell Endocrinol 188:85-97. 2002
    ....
  52. doi Tyrosine 302 in RACK1 is essential for insulin-like growth factor-I-mediated competitive binding of PP2A and beta1 integrin and for tumor cell proliferation and migration
    Patrick A Kiely
    Department of Biochemistry, Biosciences Institute, University College Cork, Ireland
    J Biol Chem 283:22952-61. 2008
    ..Taken together, the data demonstrate that Tyr-302 in RACK1 is required for interaction with PP2A and beta1 integrin, for regulation of PP2A activity, and for IGF-I-mediated cell migration and proliferation...
  53. pmc A mechanism of translational repression by competition of Paip2 with eIF4G for poly(A) binding protein (PABP) binding
    Muhammad M Karim
    Department of Biochemistry and McGill Cancer Center, McGill University, Montreal, QC, Canada H3G 1Y6
    Proc Natl Acad Sci U S A 103:9494-9. 2006
    ..Thus, Paip2 can inhibit translation by a previously unrecognized mechanism, which is independent of its ability to disrupt PABP-poly(A) interaction...
  54. ncbi The CXC chemokine SDF-1 is the ligand for LESTR/fusin and prevents infection by T-cell-line-adapted HIV-1
    E Oberlin
    Unite d Immunologie Virale, Institut Pasteur, Paris, France
    Nature 382:833-5. 1996
    ....
  55. ncbi Pesticides interfere with the nongenomic action of a progestogen on meiotic maturation by binding to its plasma membrane receptor on fish oocytes
    S Das
    Department of Internal Medicine, Yale University, School of Medicine, VA Medical Center, West Haven, CT 06516, USA
    Endocrinology 140:1953-6. 1999
    ..The close correspondence between competitive binding of the two pesticides to the 20beta-S membrane receptor and their inhibition of 20beta-S induced FOM ..
  56. ncbi PKCepsilon activation induces dichotomous cardiac phenotypes and modulates PKCepsilon-RACK interactions and RACK expression
    J M Pass
    Department of Physiology and Biophysics, University of Louisville, Louisville, Kentucky 40292, USA
    Am J Physiol Heart Circ Physiol 280:H946-55. 2001
    ..Our observations demonstrate that RACK expression is dynamically regulated by PKCepsilon and suggest that differential patterns of PKCepsilon-RACK interactions may be important determinants of PKCepsilon-dependent cardiac phenotypes...
  57. ncbi Receptor-mediated effects on ligand availability influence relative mitogenic potencies of epidermal growth factor and transforming growth factor alpha
    C C Reddy
    Department of Chemical Engineering, University of Illinois at Urbana Champaign, Urbana, Illinois 61801, USA
    J Cell Physiol 166:512-22. 1996
    ..Our results suggest a model of regulation of hormone responsiveness which favors dissociative ligands (such as TGF alpha) in receptor-limited situations and non-dissociative ligands (such as EGF) in the face of high receptor levels...
  58. pmc External TEA block of shaker K+ channels is coupled to the movement of K+ ions within the selectivity filter
    Jill Thompson
    Department of Pharmacology and Physiology, University of Rochester Medical Center, Rochester, NY 14642, USA
    J Gen Physiol 122:239-46. 2003
    ....
  59. ncbi A fluorescent intercalator displacement assay for establishing DNA binding selectivity and affinity
    Winston C Tse
    Department of Chemistry and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Acc Chem Res 37:61-9. 2004
    ....
  60. ncbi The behavior of mixtures of paralytic shellfish toxins in competitive binding assays
    Lyndon E Llewellyn
    Australian Institute of Marine Science, PMB 3, Townsville MC, Queensland, 4810, Australia
    Chem Res Toxicol 19:661-7. 2006
    ..This equation has been extended to mixtures of PSTs containing more than three toxins and may be applicable to other natural contaminants and any competitive binding assays used to detect their presence and measure their concentration.
  61. ncbi Cooperative and competitive binding in synergistic mixtures of Thermobifida fusca cellulases Cel5A, Cel6B, and Cel9A
    Tina Jeoh
    Department of Biological and Environmental Engineering and Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14853, USA
    Biotechnol Prog 18:760-9. 2002
    ....
  62. ncbi Molecular modeling of wild-type and D816V c-Kit inhibition based on ATP-competitive binding of ellipticine derivatives to tyrosine kinases
    Jeremie Vendome
    CNRS UMR 8113, Laboratoire de Biotechnologies et Pharmacologie génétique Appliquée LBPA Ecole Normale Supérieure de Cachan, 61 Avenue du President Wilson, 94235 Cachan, France
    J Med Chem 48:6194-201. 2005
    ..The model provides possible explanations for the structure-activity relationships observed among ellipticine compounds, resulting in new insights into D816V c-Kit mutant inhibition...
  63. doi Development of a competitive binding assay system with recombinant estrogen receptors from multiple species
    Cynthia V Rider
    Department of Molecular Biomedical Sciences, North Carolina State University, Raleigh, NC 27606, USA
    Toxicol Lett 184:85-9. 2009
    ..This system provides a convenient in vitro approach for directly comparing chemical binding to estrogen receptors across multiple species without the need to sacrifice animals...
  64. pmc Binding of the G domains of laminin alpha1 and alpha2 chains and perlecan to heparin, sulfatides, alpha-dystroglycan and several extracellular matrix proteins
    J F Talts
    Max Planck Institut fur Biochemie, Am Klopferspitz 18a, D 82152 Martinsried, Germany
    EMBO J 18:863-70. 1999
    ..This demonstrated similar binding repertoires for the LG modules of three basement membrane proteins involved in cell-matrix interactions and supramolecular assembly...
  65. pmc Structure-activity relationships of new 1H-imidazo[4,5-c]quinolin-4-amine derivatives as allosteric enhancers of the A3 adenosine receptor
    Anikó Göblyös
    Division of Medicinal Chemistry, Leiden Amsterdam Center for Drug Research, Leiden University, P O Box 9502, 2300 RA, Leiden, The Netherlands
    J Med Chem 49:3354-61. 2006
    ..Thus, we have prepared allosteric enhancers of the human A3AR that have an improved allosteric effect in comparison to the inhibition of equilibrium binding at the orthosteric site...
  66. pmc HEXIM1 is a promiscuous double-stranded RNA-binding protein and interacts with RNAs in addition to 7SK in cultured cells
    Qintong Li
    Department of Biochemistry, University of Iowa, Iowa City, Iowa 52242, USA
    Nucleic Acids Res 35:2503-12. 2007
    ..Our study illuminates novel properties of HEXIM1 both in vitro and in vivo, and suggests that HEXIM1 may be involved in other nuclear and cytoplasmic processes besides controlling P-TEFb...
  67. ncbi Antiobesity efficacy of a novel cannabinoid-1 receptor inverse agonist, N-[(1S,2S)-3-(4-chlorophenyl)-2-(3-cyanophenyl)-1-methylpropyl]-2-methyl-2-[[5-(trifluoromethyl)pyridin-2-yl]oxy]propanamide (MK-0364), in rodents
    Tung M Fong
    Department of Metabolic Disorders, Merck Research Laboratories, R80M 213, P O Box 2000, Rahway, NJ 07065, USA
    J Pharmacol Exp Ther 321:1013-22. 2007
    ..These studies demonstrated that MK-0364 is a highly potent and selective CB1R inverse agonist and that it is orally active in rodent models of obesity...
  68. ncbi Steroid and G protein binding characteristics of the seatrout and human progestin membrane receptor alpha subtypes and their evolutionary origins
    Peter Thomas
    University of Texas Marine Science Institute, 750 Channel View Drive, Port Aransas, Texas 78373, USA
    Endocrinology 148:705-18. 2007
    ..Thus, mPRs arose from Eubacteria independently from members of the GPCR superfamily, which arose from Archeabacteria, suggesting convergent evolution of seven-transmembrane hormone receptors coupled to G proteins...
  69. ncbi Model of competitive binding of vascular endothelial growth factor and placental growth factor to VEGF receptors on endothelial cells
    Feilim Mac Gabhann
    Department of Biomedical Engineering, School of Medicine, Johns Hopkins University, Traylor 613, 720 Rutland Ave, Baltimore, MD 21205, USA
    Am J Physiol Heart Circ Physiol 286:H153-64. 2004
    ..These results suggest that VEGFR1 signaling can be functional in adult-derived endothelial cells...
  70. pmc Tat competes with HEXIM1 to increase the active pool of P-TEFb for HIV-1 transcription
    Matjaz Barboric
    Department of Medicine, Rosalind Russell Medical Research Center, University of California at San Francisco, San Francisco, CA 94143 0703, USA
    Nucleic Acids Res 35:2003-12. 2007
    ..All these data are consistent with the model that Tat not only recruits but also increases the active pool of P-TEFb for efficient HIV-1 transcription...
  71. ncbi Synthetic cryptolepine inhibits DNA binding of NF-kappaB
    Olumayokun A Olajide
    Department of Pharmacy, Center of Drug Research, University of Munich, Butenandtstr 5 13, 81377 Munich, Germany
    Bioorg Med Chem 15:43-9. 2007
    ..This indicates that cryptolepine may exhibit its anti-inflammatory action by blocking DNA binding of activated NF-kappaB and thus transcription of NF-kappaB-regulated proinflammatory proteins...
  72. pmc Evaluation of novel antimouse VEGFR2 antibodies as potential antiangiogenic or vascular targeting agents for tumor therapy
    Sophia Ran
    Simmons Comprehensive Cancer Center, University of Texas Southwestern Medical Center, Dallas, TX 75390 8594, USA
    Neoplasia 5:297-307. 2003
    ..Vascular damage was not observed in normal organs, including kidneys and pancreas. These studies demonstrate that anti-VEGFR2 antibodies have potential for vascular targeting and imaging of tumors in vivo...
  73. ncbi Endocrine disruptor bisphenol A strongly binds to human estrogen-related receptor gamma (ERRgamma) with high constitutive activity
    Sayaka Takayanagi
    Laboratory of Structure Function Biochemistry, Department of Chemistry, Faculty and Graduate School of Sciences, Kyushu University, Fukuoka 812 8581, Japan
    Toxicol Lett 167:95-105. 2006
    ..It will now be important to evaluate whether BPA's hitherto reported low dose effects may be mediated through ERRgamma...
  74. ncbi B lymphocyte-induced maturation protein (Blimp)-1, IFN regulatory factor (IRF)-1, and IRF-2 can bind to the same regulatory sites
    Tracy C Kuo
    Department of Microbiology, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA
    J Immunol 173:5556-63. 2004
    ..Thus, our data suggest that Blimp-1 competes in vivo with a subset of IRF proteins and help predict the sites and IRF family members that may be affected...
  75. ncbi Competitive binding of pentraxins and IgM to newly exposed epitopes on late apoptotic cells
    Caroline L F Ciurana
    Department of Immunopathology, Sanquin Research at CLB, Plesmanlaan 125, 1066 CX Amsterdam, The Netherlands
    Cell Immunol 239:14-21. 2006
    ..This shared specificity as well as their shared capability to activate complement, suggest that IgM and the pentraxins CRP and SAP exert similar functions in the removal of apoptotic cells...
  76. ncbi Competitive binding of CREB and ATF2 to cAMP/ATF responsive element regulates eNOS gene expression in endothelial cells
    Kazuo Niwano
    Department of Medicine and Biological Science, Gunma University Graduate School of Medicine, Japan
    Arterioscler Thromb Vasc Biol 26:1036-42. 2006
    ..We examined the effects of Beraprost sodium (BPS), a stable analogue of prostacyclin, on the eNOS gene expression in the presence of inflammatory cytokine interleukin (IL)-1beta in cultured endothelial cells...
  77. ncbi Guanine nucleotide exchange factors operate by a simple allosteric competitive mechanism
    Zhong Guo
    Department of Structural Biology, Max Planck Institute of Molecular Physiology, Otto Hahn Strasse 11, 44227 Dortmund, Germany
    Biochemistry 44:15423-9. 2005
    ..In addition, we present arguments, which demonstrate how the erroneous conclusions leading to the alternative model were derived...
  78. ncbi [3H]N-[4-(3,4-dihydro-6,7-dimethoxyisoquinolin-2(1H)-yl)butyl]-2-methoxy-5-methylbenzamide: a novel sigma-2 receptor probe
    Jinbin Xu
    Department of Radiology, Washington University School of Medicine, 510 S Kingshighway Blvd, St Louis, MO 63110, USA
    Eur J Pharmacol 525:8-17. 2005
    ..b>Competitive binding assays were also conducted using a panel of compounds with known affinity for sigma2 receptors...
  79. ncbi Saturated fat-induced changes in Sf 60-400 particle composition reduces uptake of LDL by HepG2 cells
    Kim G Jackson
    School of Food Biosciences, University of Reading, Reading, Berkshire, United Kingdom
    J Lipid Res 47:393-403. 2006
    ..008). In conclusion, these findings suggest an alternative or additional mechanism whereby acute fat ingestion can influence LDL clearance via competitive apoE-dependent effects of TRL on the LDL receptor...
  80. ncbi Glucose sensors based on microcapsules containing an orange/red competitive binding resonance energy transfer assay
    Swetha Chinnayelka
    Biomedica Engineering Program, Institute for Micromanufacturing, Louisiana Tech University, Ruston, 71272, USA
    Diabetes Technol Ther 8:269-78. 2006
    ..A recent development towards this goal is a highly specific and sensitive competitive binding assay for glucose using apo-glucose oxidase (apo-GOx) as the recognition element and dextran as the ..
  81. ncbi Aptamers targeted to an RNA hairpin show improved specificity compared to that of complementary oligonucleotides
    Fabien Darfeuille
    INSERM U386, Universite Victor Segalen, 33076 Bordeaux, France
    Biochemistry 45:12076-82. 2006
    ..These results demonstrate the benefit of reading the three-dimensional shape of an RNA target rather than its primary sequence for the design of highly specific oligonucleotides...
  82. pmc Divalent cation sensitivity of BK channel activation supports the existence of three distinct binding sites
    Xu Hui Zeng
    Department of Anesthesiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Gen Physiol 125:273-86. 2005
    ..The major kinetic effect of the E399-related low affinity mechanism is to slow deactivation at mM Mg2+ or Ca2+. The results support the view that three distinct divalent-cation binding sites mediate regulation of BK channels...
  83. ncbi The anomalous behaviour of exogenous 25-hydroxyvitamin D in competitive binding assays
    G D Carter
    Department of Clinical Chemistry, Hammersmith Hospitals NHS Trust, London W6 8RF, UK
    J Steroid Biochem Mol Biol 103:480-2. 2007
    ..9, 46.4%, 43.2%, HPLC (n=9); 42.6, 112.2%, 97.1%, LC-MS (n=4); 34.0, 111.5%, 118.1%. The IDS RIA and Nichols assays gave unexpectedly low recoveries. This does not appear to be a calibration problem or the effect of ethanol...
  84. ncbi Satellite DNA binding and cellular localisation of RNA helicase P68
    Natella Enukashvily
    Cell Cultures Department, Institute of Cytology, Tikhoretsky, 4, St Petersburg, 194064, Russia
    J Cell Sci 118:611-22. 2005
    ..Direct interaction of P68/DEAD box RNA helicase with satellite DNAs in vitro has not been demonstrated for any other members of the RNA helicase family...
  85. pmc Maintenance of antigen-specific immunological memory through variable regions of heavy and light chains of anti-idiotypic antibody
    J Vani
    Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, Karnataka, India
    Immunology 120:486-96. 2007
    ..Evidence has been provided for the existence of an antigen-specific B-cell idiotypic network in the body that supports the perpetuation of immunological memory as proposed in the relay hypothesis...
  86. pmc Aha1 competes with Hop, p50 and p23 for binding to the molecular chaperone Hsp90 and contributes to kinase and hormone receptor activation
    Anja Harst
    Protein Folding Group, Institute for Genetics, University of Bonn, Römerstr 164, D 53117 Bonn, Germany
    Biochem J 387:789-96. 2005
    ....
  87. ncbi Design and synthesis of an expanded porphyrin that has selectivity for the c-MYC G-quadruplex structure
    Jeyaprakashnarayanan Seenisamy
    College of Pharmacy, The University of Arizona, 1703 East Mabel, Tucson, Arizona 85721, USA
    J Am Chem Soc 127:2944-59. 2005
    ..From this study, we have identified an expanded porphyrin that selectively binds with the c-MYC G-quadruplex in the presence of duplex DNA and other G-quadruplexes...
  88. pmc TIP47 is a key effector for Rab9 localization
    Dikran Aivazian
    Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305, USA
    J Cell Biol 173:917-26. 2006
    ..These data confirm the importance of effector interactions for Rab9 localization, and support a model in which effector proteins rely on Rabs as much as Rabs rely on effectors to achieve their correct steady state localizations...
  89. ncbi Influence of surface shape on DNA binding of bimetallo helicates
    Jemma C Peberdy
    Department of Chemistry, University of Warwick, Coventry CV4 7AL, UK
    J Inorg Biochem 101:1937-45. 2007
    ..b>Competitive binding studies using fluorescent dyes Hoechst 33258 (a minor groove binder), ethidium bromide (an intercalator) ..
  90. pmc Ca2+-dependent inactivation of CaV1.2 channels prevents Gd3+ block: does Ca2+ block the pore of inactivated channels?
    Olga Babich
    Department of Pharmacology and Physiology, University of Medicine and Dentistry of New Jersey, New Jersey Medical School, Newark, NJ 07103, USA
    J Gen Physiol 129:477-83. 2007
    ..A minimal model, in which the inactivation "gate" is an increase in affinity of the selectivity filter for permeating ions, successfully simulates the characteristic U-shaped voltage dependence of inactivation in Ca(2+)...
  91. ncbi Small molecule inhibitors of the MDM2-p53 interaction discovered by ensemble-based receptor models
    Anna L Bowman
    Department of Medicinal Chemistry and the Comprehensive Cancer Center, University of Michigan, Ann Arbor, MI 48109, USA
    J Am Chem Soc 129:12809-14. 2007
    ..Our MPS technique is one of very few ensemble-based techniques to be proven through experimental verification of the discovery of new inhibitors...
  92. pmc Block of CaV1.2 channels by Gd3+ reveals preopening transitions in the selectivity filter
    Olga Babich
    Department of Pharmacology and Physiology, University of Medicine and Dentistry of New Jersey, New Jersey Medical School, Newark, NJ 07103, USA
    J Gen Physiol 129:461-75. 2007
    ..Therefore, we propose that at submicromolar concentrations, Gd(3+) binds at the entry to the selectivity locus and that the affinity of the site for permeant ions decreases during preopening transitions of the channel...
  93. ncbi Androgen binding profiles of two distinct nuclear androgen receptors in Atlantic croaker (Micropogonias undulatus)
    T S Sperry
    Department of Marine Science, The University of Texas Marine Science Institute, The University of Texas at Austin, 78373, USA
    J Steroid Biochem Mol Biol 73:93-103. 2000
    ..Atlantic croaker (Micropogonias undulatus) brain and ovarian tissues, respectively, were determined using competitive binding assays...
  94. ncbi Development of high throughput screening assays using fluorescence polarization: nuclear receptor-ligand-binding and kinase/phosphatase assays
    G J Parker
    PanVera Corporation, Madison, WI 53711, USA
    J Biomol Screen 5:77-88. 2000
    ..These data show that an FP-based competitive binding assay can be used to screen diverse compounds with a broad range of binding affinities for ERs...
  95. ncbi Differential binding affinities of PCBs, HO-PCBs, and aroclors with recombinant human, rainbow trout (Onchorhynkiss mykiss), and green anole (Anolis carolinensis) estrogen receptors, using a semi-high throughput competitive binding assay
    J Matthews
    Department of Biochemistry and National Food Safety and Toxicology Center, Michigan State University, East Lansing 48824 1319, USA
    Toxicol Sci 53:326-39. 2000
    ..These results demonstrate that ERs from different species exhibit differential ligand preferences and relative binding affinities for PCBs, which can be dramatically affected by DMSO concentration...
  96. ncbi Competitive binding of AUF1 and TIAR to MYC mRNA controls its translation
    Baisong Liao
    Department of Molecular Genetics, Microbiology, and Immunology, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, 675 Hoes Lane, Piscataway, New Jersey 08854, USA
    Nat Struct Mol Biol 14:511-8. 2007
    ..Together, these observations suggest a novel regulatory mechanism where tuning the ratios of AUF1 and TIAR bound to MYC mRNA permits dynamic control of MYC translation and cell proliferation...
  97. ncbi In vitro and in vivo binding of neuroactive steroids to the sigma-1 receptor as measured with the positron emission tomography radioligand [18F]FPS
    Rikki N Waterhouse
    Department of Psychiatry, Columbia University, New York, New York, USA
    Synapse 61:540-6. 2007
    ....
  98. doi The Orf18 gene product from conjugative transposon Tn916 is an ArdA antirestriction protein that inhibits type I DNA restriction-modification systems
    Dimitra Serfiotis-Mitsa
    School of Chemistry, The University of Edinburgh, The King s Buildings, Edinburgh EH9 3JJ, UK
    J Mol Biol 383:970-81. 2008
    ..Our results suggest that ArdA can overcome the restriction barrier following conjugation and so helps increase the spread of antibiotic resistance genes by horizontal gene transfer...
  99. doi Host-guest chemistry of dendrimer-drug complexes. 3. Competitive binding of multiple drugs by a single dendrimer for combination therapy
    Libo Zhao
    Laboratory of Functional Membranes, Department of Chemistry, University of Science and Technology of China, Hefei, Anhui 230026, PR China
    J Phys Chem B 113:14172-9. 2009
    ..The competitive binding of multiple drugs by a single dendrimer in aqueous solutions was investigated by (1)H NMR and 2D-NOESY ..
  100. ncbi Vitronectin binding to IGF binding protein-5 (IGFBP-5) alters IGFBP-5 modulation of IGF-I actions
    Taek Nam
    Division of Endocrinology, University of North Carolina, Chapel Hill, NC 27599, USA
    Endocrinology 143:30-6. 2002
    ..Synthetic peptides that contained four distinct regions of the IGFBP-5 sequence were used in competitive binding assays to determine the regions of IGFBP-5 that were necessary for vitronectin binding...
  101. ncbi Competitive binding of xenobiotic oestrogens to rat alpha-fetoprotein and to sex steroid binding proteins in human and rainbow trout (Oncorhynchus mykiss) plasma
    S R Milligan
    Physiology Group, Biomedical Sciences Division, King s College London, Strand, London, WC2R 2LS, United Kingdom
    Gen Comp Endocrinol 112:89-95. 1998
    ..These results suggest that environmental oestrogenic agents are unlikely to produce biological effects by displacing endogenous steroids from plasma steroid binding proteins unless they are present in very high concentrations...

Research Grants75

  1. Dual Direct Inhibitors of Thrombin and Factor Xa
    Umesh Desai; Fiscal Year: 2009
    ..7) Competitive binding studies suggest that CDs interacts with exosite II of thrombin, a site not typically associated with ..
  2. Dual Direct Inhibitors of Thrombin and Factor Xa
    UMESH RAMANLAL DESAI; Fiscal Year: 2010
    ..7) Competitive binding studies suggest that CDs interacts with exosite II of thrombin, a site not typically associated with ..
  3. METALLOPROTEASES IN CONNECTIVE TISSUE MATRIX BREAKDOWN
    Hideaki Nagase; Fiscal Year: 1993
    ..to tissue inhibitor of metalloproteinases 1 (TIMP-1) and in reaction with alpha2- macroglobulin by a competitive binding "label selection" method...
  4. Novel genomic effects of Y-linked polymorphisms
    Daniel L Hartl; Fiscal Year: 2010
    ..population dynamics of polymorphic Y chromosomes associated with YRV to determine whether a model based on competitive binding is possible with realistic values of the parameters...
  5. Biotransformation of Estrogens to Carcinogenic Quinoids
    JUDY BOLTON; Fiscal Year: 2005
    ..The relative binding affinity of these compounds to ERa and ERb proteins will be measured using a competitive binding assay...
  6. FACTOR VA INTERACTIONS REGULATING PROTHROMBIN ACTIVATION
    Paul Bock; Fiscal Year: 2007
    ..inactivation by APC through ordered cleavages at Arg506, Arg306, and Arg679 is achieved by exosite-mediated competitive binding of factor Xa and APC to factor Va for cleavage at Arg506, and specific acceleration of cleavage at Arg306 ..
  7. FACTOR VA INTERACTIONS REGULATING PROTHROMBIN ACTIVATION
    Paul E Bock; Fiscal Year: 2010
    ..inactivation by APC through ordered cleavages at Arg506, Arg306, and Arg679 is achieved by exosite-mediated competitive binding of factor Xa and APC to factor Va for cleavage at Arg506, and specific acceleration of cleavage at Arg306 ..
  8. INTESTINAL AND PLACENTAL FC-RECEPTORS FOR IMMUNOGLOBULIN
    Neil Simister; Fiscal Year: 1999
    ..The specificity of human FcRn for different human IgG subclasses, measured by a competitive binding assay, will be compared with the relative efficiency of placental transport of these subclasses...
  9. NON-H-2 HISTOCOMPATIBILITY GENES AND ENCODED ANTIGENS
    Peter Wettstein; Fiscal Year: 1993
    ..viral and soluble antigens, and abundant endogenous proteins will be tested for relative affinity in competitive binding assays and assays for stabilization of expression of class I:peptide complexes...
  10. STRUCTURE & FUNCTION OF E HISTOLYTICA ADHERENCE LECTIN
    William Petri; Fiscal Year: 1992
    ..Antigenic determinants and functional domains of the adherence lectin will be defined by competitive binding of monoclonal antibodies...
  11. Serpin Regulation of Coagulation Proteases
    Alireza R Rezaie; Fiscal Year: 2010
    ..These studies will utilize enzyme kinetics, fluorescence spectroscopy, plasmon resonance, and competitive binding methods to provide critical information about kinetic and thermodynamic constants that define the ..
  12. Serpin Regulation of Coagulation Proteases
    ALIREZA REZAIE; Fiscal Year: 2009
    ..These studies will utilize enzyme kinetics, fluorescence spectroscopy, plasmon resonance, and competitive binding methods to provide critical information about kinetic and thermodynamic constants that define the ..
  13. STRUCTURE/FUNCTION OF F-VIII-WILLEBRAND FACTOR COMPLEX
    JOHN LOLLAR; Fiscal Year: 1992
    ..This technique will allow determination of whether fVIIIa binds WF and by competitive binding studies, which subunits or fragments of fVIII bind WF...
  14. Y Receptor In and Cardiovascular Function From the PHN
    John Martin; Fiscal Year: 2004
    ..IP3 levels will be measured using competitive binding between [3H]IP3 and IP3 from the tissue slices to a binding protein...
  15. MOLECULAR BASIS OF COAGULATION PROTEASE SPECIFICITY
    Alireza R Rezaie; Fiscal Year: 2010
    ..These studies will utilize fluorescence spectroscopy, enzyme kinetics, and competitive binding methods to provide critical information about the kinetic and thermodynamic constants that define the ..
  16. MOLECULAR BASIS OF COAGULATION PROTEASE SPECIFICITY
    ALIREZA REZAIE; Fiscal Year: 2009
    ..These studies will utilize fluorescence spectroscopy, enzyme kinetics, and competitive binding methods to provide critical information about the kinetic and thermodynamic constants that define the ..
  17. MOLECULAR BASIS OF COAGULATION PROTEASE SPECIFICITY
    ALIREZA REZAIE; Fiscal Year: 2007
    ..These studies will utilize fluorescence spectroscopy, enzyme kinetics, and competitive binding methods to provide critical information about the kinetic and thermodynamic constants that define the ..
  18. Functional assessment of methylation-associted nuclear proteins in pancreatic ade
    TALAL ELHEFNAWY; Fiscal Year: 2007
    ..In one model, the DNA methyl transferase target-specificity is influenced by the promoter competitive binding to differentially expressed nuclear proteins thus maintaining the hypomethylated status of the DNA binding ..
  19. Development of Translation Initiation Inhibitors and their Anti-Cancer Activity
    Michael Chorev; Fiscal Year: 2010
    ..b>competitive binding to eIF4E, 2. inhibition of cancer cell growth, 3...
  20. PRO LEU GLY NH2 AMD DOPAMINE RECEPTOR MODULATION STUDY
    Rodney Johnson; Fiscal Year: 1999
    ..synthesized will be evaluated for their ability to modulate (3H]spiroperidol/N- propylnorapomorphine competitive binding to D2 receptors in the presence or absence of 5 prime-guanylylimidodiphosphate, a norhydrolyzable analog of ..