competitive binding

Summary

Top Publications

  1. ncbi Nicotinic acetylcholine receptors on basophils and mast cells
    P S Sudheer
    Department of Anaesthetics and Intensive Care Medicine, University Hospital of Wales, Cardiff, UK
    Anaesthesia 61:1170-4. 2006
  2. ncbi Recombinant transthyretin purification and competitive binding with organohalogen compounds in two gull species (Larus argentatus and Larus hyperboreus)
    Francisco Ucán-Marin
    National Wildlife Research Centre, Carleton University, Ottawa, Ontario, K1A 0H3, Canada
    Toxicol Sci 107:440-50. 2009
  3. ncbi Competitive binding of poly- and perfluorinated compounds to the thyroid hormone transport protein transthyretin
    Jana M Weiss
    Institute for Environmental Studies, Department of Chemistry and Biology, VU University, 1081 HV Amsterdam, The Netherlands
    Toxicol Sci 109:206-16. 2009
  4. ncbi Tyrosine 302 in RACK1 is essential for insulin-like growth factor-I-mediated competitive binding of PP2A and beta1 integrin and for tumor cell proliferation and migration
    Patrick A Kiely
    Department of Biochemistry, Biosciences Institute, University College Cork, Ireland
    J Biol Chem 283:22952-61. 2008
  5. pmc Polyamines regulate the stability of JunD mRNA by modulating the competitive binding of its 3' untranslated region to HuR and AUF1
    Tongtong Zou
    Department of Surgery, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
    Mol Cell Biol 30:5021-32. 2010
  6. pmc TIP47 is a key effector for Rab9 localization
    Dikran Aivazian
    Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305, USA
    J Cell Biol 173:917-26. 2006
  7. ncbi A Dimer of Escherichia coli UvrD is the active form of the helicase in vitro
    Nasib K Maluf
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, 660 S Euclid Avenue, Box 8231, St Louis, MO 63110 1093, USA
    J Mol Biol 325:913-35. 2003
  8. pmc Endogenous expression of a high-affinity pseudoknot RNA aptamer suppresses replication of HIV-1
    Laurent Chaloin
    Max Planck Institut fur molekulare Physiologie, Abteilung Physikalische Biochemie, Otto Hahn Strasse 11, 44227 Dortmund, Germany
    Nucleic Acids Res 30:4001-8. 2002
  9. pmc PUMA promotes Bax translocation by both directly interacting with Bax and by competitive binding to Bcl-X L during UV-induced apoptosis
    Yingjie Zhang
    Ministry of Education Key Laboratory of Laser Life Science and Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China
    Mol Biol Cell 20:3077-87. 2009
  10. ncbi Mechanism of Cd2+ toxicity: Cd2+ inhibits photoactivation of Photosystem II by competitive binding to the essential Ca2+ site
    Peter Faller
    Institut fur Biologie II, Biochemie der Pflanzen, Universitat Freiburg, Schänzlestr 1, 79104 Freiburg, Germany
    Biochim Biophys Acta 1706:158-64. 2005

Detail Information

Publications245 found, 100 shown here

  1. ncbi Nicotinic acetylcholine receptors on basophils and mast cells
    P S Sudheer
    Department of Anaesthetics and Intensive Care Medicine, University Hospital of Wales, Cardiff, UK
    Anaesthesia 61:1170-4. 2006
    ..The identity of this receptor was confirmed by reverse transcriptase PCR and quantitative PCR...
  2. ncbi Recombinant transthyretin purification and competitive binding with organohalogen compounds in two gull species (Larus argentatus and Larus hyperboreus)
    Francisco Ucán-Marin
    National Wildlife Research Centre, Carleton University, Ottawa, Ontario, K1A 0H3, Canada
    Toxicol Sci 107:440-50. 2009
    ..the OH-substituted analogs all had lower K(i) and K(d) values, indicating greater affinity and more potent competitive binding to both T(3) and T(4)...
  3. ncbi Competitive binding of poly- and perfluorinated compounds to the thyroid hormone transport protein transthyretin
    Jana M Weiss
    Institute for Environmental Studies, Department of Chemistry and Biology, VU University, 1081 HV Amsterdam, The Netherlands
    Toxicol Sci 109:206-16. 2009
    ..b>Competitive binding of PFCs to TTR, as observed for human TTR in the present study, may explain altered thyroid hormone levels ..
  4. ncbi Tyrosine 302 in RACK1 is essential for insulin-like growth factor-I-mediated competitive binding of PP2A and beta1 integrin and for tumor cell proliferation and migration
    Patrick A Kiely
    Department of Biochemistry, Biosciences Institute, University College Cork, Ireland
    J Biol Chem 283:22952-61. 2008
    ..Taken together, the data demonstrate that Tyr-302 in RACK1 is required for interaction with PP2A and beta1 integrin, for regulation of PP2A activity, and for IGF-I-mediated cell migration and proliferation...
  5. pmc Polyamines regulate the stability of JunD mRNA by modulating the competitive binding of its 3' untranslated region to HuR and AUF1
    Tongtong Zou
    Department of Surgery, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
    Mol Cell Biol 30:5021-32. 2010
    ..JunD mRNA is a target of HuR and AUF1 and that polyamines modulate JunD mRNA degradation by altering the competitive binding of HuR and AUF1 to the JunD 3'-UTR...
  6. pmc TIP47 is a key effector for Rab9 localization
    Dikran Aivazian
    Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305, USA
    J Cell Biol 173:917-26. 2006
    ..These data confirm the importance of effector interactions for Rab9 localization, and support a model in which effector proteins rely on Rabs as much as Rabs rely on effectors to achieve their correct steady state localizations...
  7. ncbi A Dimer of Escherichia coli UvrD is the active form of the helicase in vitro
    Nasib K Maluf
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, 660 S Euclid Avenue, Box 8231, St Louis, MO 63110 1093, USA
    J Mol Biol 325:913-35. 2003
    ..Initiation of DNA unwinding in vitro appears to require a dimeric UvrD complex in which one subunit is bound to the ssDNA/dsDNA junction, while the second subunit is bound to the 3' ssDNA tail...
  8. pmc Endogenous expression of a high-affinity pseudoknot RNA aptamer suppresses replication of HIV-1
    Laurent Chaloin
    Max Planck Institut fur molekulare Physiologie, Abteilung Physikalische Biochemie, Otto Hahn Strasse 11, 44227 Dortmund, Germany
    Nucleic Acids Res 30:4001-8. 2002
    ..This is the first example of an RNA aptamer selected against a viral enzyme target to show powerful antiviral activity in HIV-1-permissive human T-lymphoid cell lines...
  9. pmc PUMA promotes Bax translocation by both directly interacting with Bax and by competitive binding to Bcl-X L during UV-induced apoptosis
    Yingjie Zhang
    Ministry of Education Key Laboratory of Laser Life Science and Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China
    Mol Biol Cell 20:3077-87. 2009
    ..Together, these studies create an important conclusion that PUMA promotes Bax translocation by both by directly interacting with Bax and by competitive binding to Bcl-X(L) in UV-induced apoptosis.
  10. ncbi Mechanism of Cd2+ toxicity: Cd2+ inhibits photoactivation of Photosystem II by competitive binding to the essential Ca2+ site
    Peter Faller
    Institut fur Biologie II, Biochemie der Pflanzen, Universitat Freiburg, Schänzlestr 1, 79104 Freiburg, Germany
    Biochim Biophys Acta 1706:158-64. 2005
    ..This mechanism is likely to be important for Cd2+ toxicity towards photosynthetic organisms in general, at least in unicellular like C. reinhardtii where Cd2+ has easy access to the photosynthetic apparatus...
  11. ncbi Identification of hSRP1 alpha as a functional receptor for nuclear localization sequences
    K Weis
    European Molecular Biology Laboratory, Heidelberg, Germany
    Science 268:1049-53. 1995
    ..Thus, hSRP1 alpha has the properties of a cytosolic receptor for both simple and bipartite NLS motifs...
  12. ncbi Epitope mapping and competitive binding of HSA drug site II ligands by NMR diffusion measurements
    Laura H Lucas
    Department of Chemistry, University of Kansas, Lawrence, Kansas 66045, USA
    J Am Chem Soc 126:14258-66. 2004
    ....
  13. pmc Elucidating the relationship between DISC1, NDEL1 and NDE1 and the risk for schizophrenia: evidence of epistasis and competitive binding
    Katherine E Burdick
    Department of Psychiatry Research, The Zucker Hillside Hospital, North Shore Long Island Jewish Health System, Glen Oaks, NY 11004, USA
    Hum Mol Genet 17:2462-73. 2008
    ..These data suggest that NDEL1 significantly influences risk for SZ via an interaction with DISC1. We propose a model where NDEL1 and NDE1 compete for binding with DISC1...
  14. ncbi Competitive binding of alpha-actinin and calmodulin to the NMDA receptor
    M Wyszynski
    Howard Hughes Medical Institute, Harvard Medical School, Boston, Massachusetts 02114, USA
    Nature 385:439-42. 1997
    ..Intriguingly, NR1-alpha-actinin binding is directly antagonized by Ca2+/calmodulin. Thus alpha-actinin may play a role in both the localization of NMDA receptors and their modulation by Ca2+...
  15. ncbi Competitive binding assay for glucose based on glycodendrimer-fluorophore conjugates
    Bennett L Ibey
    Department of Biomedical Engineering, Texas A and M University, College Station, Texas 77843, USA
    Anal Chem 77:7039-46. 2005
    ..Data showing that the sensor is fully reversible and specific through competitive binding between the dendrimer and glucose with Con A are presented...
  16. ncbi RAC-3 is a NF-kappa B coactivator
    S Werbajh
    Deparmento de Cs Biológicas, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellon II, Argentina
    FEBS Lett 485:195-9. 2000
    ..The competition between GR and NF-kappa B for binding to RAC3 may represent a general mechanism by which both transcription factors mutually antagonize their activity...
  17. pmc A mechanism of translational repression by competition of Paip2 with eIF4G for poly(A) binding protein (PABP) binding
    Muhammad M Karim
    Department of Biochemistry and McGill Cancer Center, McGill University, Montreal, QC, Canada H3G 1Y6
    Proc Natl Acad Sci U S A 103:9494-9. 2006
    ..Thus, Paip2 can inhibit translation by a previously unrecognized mechanism, which is independent of its ability to disrupt PABP-poly(A) interaction...
  18. ncbi Design and synthesis of an expanded porphyrin that has selectivity for the c-MYC G-quadruplex structure
    Jeyaprakashnarayanan Seenisamy
    College of Pharmacy, The University of Arizona, 1703 East Mabel, Tucson, Arizona 85721, USA
    J Am Chem Soc 127:2944-59. 2005
    ..From this study, we have identified an expanded porphyrin that selectively binds with the c-MYC G-quadruplex in the presence of duplex DNA and other G-quadruplexes...
  19. ncbi Rat liver and kidney contain high densities of sigma 1 and sigma 2 receptors: characterization by ligand binding and photoaffinity labeling
    S B Hellewell
    Unit on Receptor Biochemistry and Pharmacology, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, MD 20892
    Eur J Pharmacol 268:9-18. 1994
    ..These data are consistent with the notion that the 25 kDa and 21.5 kDa proteins represent sigma 1 and sigma 2 receptors, respectively.(ABSTRACT TRUNCATED AT 400 WORDS)..
  20. ncbi New multifunctional molecular conjugate vector for targeting, imaging, and therapy of tumors
    Elisabeth Garanger
    Groupe de Recherche sur le Cancer du Poumon, INSERM U578, Institut Albert Bonniot, La Tronche 38706 Cedex, France
    Mol Ther 12:1168-75. 2005
    ....
  21. ncbi Avidity regulation of integrins: the driving force in leukocyte adhesion
    Y van Kooyk
    Department of Tumor Immunology, University Medical Center Nijmegen, St Radboud, P Van Leydenlaan 25, 6525 EX, The, Nijmegen, Netherlands
    Curr Opin Cell Biol 12:542-7. 2000
    ....
  22. ncbi A 26 S protease subunit that binds ubiquitin conjugates
    Q Deveraux
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City 84132
    J Biol Chem 269:7059-61. 1994
    ..Thus, proteins conjugated to polymers of ubiquitin may be selected for degradation by a single subunit of the 26 S protease complex...
  23. ncbi Prediction of ligand binding affinity and orientation of xenoestrogens to the estrogen receptor by molecular dynamics simulations and the linear interaction energy method
    Marola M H van Lipzig
    Leiden Amsterdam Center for Drug Research, Division of Molecular Toxicology and Division of Molecular Pharmacology, Vrije Universiteit Amsterdam, Amsterdam, The Netherlands
    J Med Chem 47:1018-30. 2004
    ..61 +/- 0.4 kcal/mol). In conclusion, our LIE model provides a very good method for prediction of absolute ligand binding affinities, as well as binding orientation of ligands...
  24. pmc Aha1 competes with Hop, p50 and p23 for binding to the molecular chaperone Hsp90 and contributes to kinase and hormone receptor activation
    Anja Harst
    Protein Folding Group, Institute for Genetics, University of Bonn, Römerstr 164, D 53117 Bonn, Germany
    Biochem J 387:789-96. 2005
    ....
  25. pmc Assessing environmental chemicals for estrogenicity using a combination of in vitro and in vivo assays
    M D Shelby
    Reproductive Toxicology Group, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA
    Environ Health Perspect 104:1296-300. 1996
    ..The assays were 1) competitive binding with the mouse uterine estrogen receptor, 2) transcriptional activation in HeLa cells transfected with ..
  26. ncbi Opposing effects of Ndel1 and alpha1 or alpha2 on cytoplasmic dynein through competitive binding to Lis1
    Chong Ding
    Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China
    J Cell Sci 122:2820-7. 2009
    ..They also help to explain why brain development is particularly sensitive to a decrease in Lis1 levels...
  27. ncbi Possible existence of common internalization mechanisms among arginine-rich peptides
    Tomoki Suzuki
    Institute for Chemical Research, Kyoto University, Uji, Kyoto 611 0011, Japan
    J Biol Chem 277:2437-43. 2002
    ..These results strongly suggest that the arginine-rich basic peptides share a certain part of the internalization pathway...
  28. ncbi Changes in conformation and subcellular distribution of alpha4beta2 nicotinic acetylcholine receptors revealed by chronic nicotine treatment and expression of subunit chimeras
    Patricia C Harkness
    Department of Pharmacology, University College London, London, WC1E 6BT, United Kingdom
    J Neurosci 22:10172-81. 2002
    ..The finding that subunit conformation can be influenced by coassembled subunit partners is in agreement with models of receptor assembly which propose that subunit folding continues after initial subunit-subunit interactions...
  29. pmc Tat competes with HEXIM1 to increase the active pool of P-TEFb for HIV-1 transcription
    Matjaz Barboric
    Department of Medicine, Rosalind Russell Medical Research Center, University of California at San Francisco, San Francisco, CA 94143 0703, USA
    Nucleic Acids Res 35:2003-12. 2007
    ..All these data are consistent with the model that Tat not only recruits but also increases the active pool of P-TEFb for efficient HIV-1 transcription...
  30. pmc Local conformations and competitive binding affinities of single- and double-stranded primer-template DNA at the polymerization and editing active sites of DNA polymerases
    Kausiki Datta
    Institute of Molecular Biology and Department of Chemistry, University of Oregon, Eugene, Oregon 97403 1229, USA
    J Biol Chem 284:17180-93. 2009
    ..These distributions and the conformational changes in the P/T DNA that occur during template-directed DNA synthesis in solution illuminate some of the mechanisms used by DNA polymerases to assure the fidelity of DNA synthesis...
  31. ncbi Molecular cloning, functional expression, and signaling characteristics of a C-C chemokine receptor
    K Neote
    Department of Immunology, Genentech, Incorporated, South San Francisco, California 94080
    Cell 72:415-25. 1993
    ..Finally, C-C chemokines were shown to bind a C-C CKR-1-related gene product encoded by cytomegalovirus, suggesting a role for C-C chemokines in viral immunity...
  32. ncbi Cloning of complementary DNA encoding a functional human interleukin-8 receptor
    P M Murphy
    Laboratory of Host Defenses, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892
    Science 253:1280-3. 1991
    ....
  33. ncbi Syntaxin 5 is a common component of the NSF- and p97-mediated reassembly pathways of Golgi cisternae from mitotic Golgi fragments in vitro
    C Rabouille
    Cell Biology Laboratory, Imperial Cancer Research Fund, London, United Kingdom
    Cell 92:603-10. 1998
    ..p47 also mediates the binding of p97 to syntaxin 5 and so plays an analogous role to alpha-SNAP, which mediates the binding of NSF...
  34. ncbi CD4-induced interaction of primary HIV-1 gp120 glycoproteins with the chemokine receptor CCR-5
    L Wu
    LeukoSite, Inc, Cambridge, Massachusetts 02142, USA
    Nature 384:179-83. 1996
    ..These results suggest that HIV-1 attachment to CD4 creates a high-affinity binding site for CCR-5, leading to membrane fusion and virus entry...
  35. pmc Dual interactions of the translational repressor Paip2 with poly(A) binding protein
    K Khaleghpour
    Department of Biochemistry and McGill Cancer Center, McGill University, Montreal, Quebec, Canada H3G 1Y6
    Mol Cell Biol 21:5200-13. 2001
    ..Significantly, only the central Paip2 fragment, which binds with high affinity to the PABP RRM region, inhibits PABP binding to poly(A) RNA and translation...
  36. ncbi Function of sigma1 receptors in Parkinson's disease
    M Mishina
    The Department of Neurology, Neurological Institute, Nippon Medical School Chiba Hokusoh Hospital, Inba gun, Chiba, Japan
    Acta Neurol Scand 112:103-7. 2005
    ....
  37. ncbi Synthetic cryptolepine inhibits DNA binding of NF-kappaB
    Olumayokun A Olajide
    Department of Pharmacy, Center of Drug Research, University of Munich, Butenandtstr 5 13, 81377 Munich, Germany
    Bioorg Med Chem 15:43-9. 2007
    ..This indicates that cryptolepine may exhibit its anti-inflammatory action by blocking DNA binding of activated NF-kappaB and thus transcription of NF-kappaB-regulated proinflammatory proteins...
  38. ncbi Endocrine disruptor bisphenol A strongly binds to human estrogen-related receptor gamma (ERRgamma) with high constitutive activity
    Sayaka Takayanagi
    Laboratory of Structure Function Biochemistry, Department of Chemistry, Faculty and Graduate School of Sciences, Kyushu University, Fukuoka 812 8581, Japan
    Toxicol Lett 167:95-105. 2006
    ..It will now be important to evaluate whether BPA's hitherto reported low dose effects may be mediated through ERRgamma...
  39. ncbi Identification of prion inhibitors by a fluorescence-polarization-based competitive binding assay
    David A Kocisko
    Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA
    Anal Biochem 363:154-6. 2007
  40. ncbi Guanine nucleotide exchange factors operate by a simple allosteric competitive mechanism
    Zhong Guo
    Department of Structural Biology, Max Planck Institute of Molecular Physiology, Otto Hahn Strasse 11, 44227 Dortmund, Germany
    Biochemistry 44:15423-9. 2005
    ..In addition, we present arguments, which demonstrate how the erroneous conclusions leading to the alternative model were derived...
  41. ncbi B lymphocyte-induced maturation protein (Blimp)-1, IFN regulatory factor (IRF)-1, and IRF-2 can bind to the same regulatory sites
    Tracy C Kuo
    Department of Microbiology, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA
    J Immunol 173:5556-63. 2004
    ..Thus, our data suggest that Blimp-1 competes in vivo with a subset of IRF proteins and help predict the sites and IRF family members that may be affected...
  42. ncbi Biosensor analysis of drug-target interactions: direct and competitive binding assays for investigation of interactions between thrombin and thrombin inhibitors
    R Karlsson
    Biacore AB, Rapsgatan 7, Uppsala, SE 754 50, Sweden
    Anal Biochem 278:1-13. 2000
    ..One additional compound was scored as positive. The direct binding assay compared favorably with two competitive assay formats, a surface competitive assay and a inhibitor in solution assay, that were examined in parallel...
  43. ncbi [3H]N-[4-(3,4-dihydro-6,7-dimethoxyisoquinolin-2(1H)-yl)butyl]-2-methoxy-5-methylbenzamide: a novel sigma-2 receptor probe
    Jinbin Xu
    Department of Radiology, Washington University School of Medicine, 510 S Kingshighway Blvd, St Louis, MO 63110, USA
    Eur J Pharmacol 525:8-17. 2005
    ..b>Competitive binding assays were also conducted using a panel of compounds with known affinity for sigma2 receptors...
  44. ncbi Competitive binding of AUF1 and TIAR to MYC mRNA controls its translation
    Baisong Liao
    Department of Molecular Genetics, Microbiology, and Immunology, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, 675 Hoes Lane, Piscataway, New Jersey 08854, USA
    Nat Struct Mol Biol 14:511-8. 2007
    ..Together, these observations suggest a novel regulatory mechanism where tuning the ratios of AUF1 and TIAR bound to MYC mRNA permits dynamic control of MYC translation and cell proliferation...
  45. pmc Hazard and risk assessment of chemical mixtures using the toxic equivalency factor approach
    S H Safe
    Department of Veterinary Physiology and Pharmacology, Texas A and M University, College Station 77843 4466, USA
    Environ Health Perspect 106:1051-8. 1998
    ....
  46. ncbi The steroid-responsive hiccup reflex arc: competitive binding to the corticosteroid-receptor?
    Rob D Dickerman
    Department of Neurosurgery, North Shore University Hospital, New Hyde Park, NY, USA
    Neuro Endocrinol Lett 24:167-9. 2003
    ..This report validates the previous report on anabolic steroids inducing hiccups and exemplifies the ability for steroids as a class, due to there backbone structural homology, to induce function even as competitive inhibitors...
  47. ncbi Influence of surface shape on DNA binding of bimetallo helicates
    Jemma C Peberdy
    Department of Chemistry, University of Warwick, Coventry CV4 7AL, UK
    J Inorg Biochem 101:1937-45. 2007
    ..b>Competitive binding studies using fluorescent dyes Hoechst 33258 (a minor groove binder), ethidium bromide (an intercalator) ..
  48. ncbi Poly(ADP-ribose) polymerase-1 is a negative regulator of HIV-1 transcription through competitive binding to TAR RNA with Tat.positive transcription elongation factor b (p-TEFb) complex
    Marianne Parent
    Division of Health and Environmental Research, Laval University Medical Center, Ste Foy, Quebec G1V 4G2, Canada
    J Biol Chem 280:448-57. 2005
    ..Thus, these results suggest that PARP-1 acts as a negative regulator of HIV-1 transcription through competitive binding with Tat or the Tat.P-TEFb complex to TAR RNA.
  49. ncbi Fab antibodies capable of blocking T cells by competitive binding have the identical specificity but a higher affinity to the MHC-peptide-complex than the T cell receptor
    Frank Neumann
    I Med Klinik, Saarland University Medical School, 66421 Homburg Saar, Germany
    Immunol Lett 125:86-92. 2009
    ..These results indicate that molecules that block T cells by competitive binding with the TCR must have the same specificity but higher affinity for the MHC-peptide-complex than the TCR.
  50. ncbi Competitive binding of ibuprofen and naproxen to bovine serum albumin : modified form of drug-drug displacement interaction at the binding site
    M M Rahman
    Pharmacy Discipline, Life Science School, Khulna University, Khulna 9208, Bangladesh
    Pak J Pharm Sci 18:43-7. 2005
    The competitive binding characteristics of ibuprofen and naproxen with respect to binding site on bovine serum albumin (BSA) was studied by equilibrium dialysis method at pH 7.4 and 25 degrees C...
  51. ncbi Proteasome subunit Rpn1 binds ubiquitin-like protein domains
    Suzanne Elsasser
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Nat Cell Biol 4:725-30. 2002
    ..We also report that, contrary to expectation, the base subunit Rpn10 does not mediate the binding of UBL proteins to the proteasome in yeast, although it can apparently contribute to the binding of ubiquitin chains by intact proteasomes...
  52. ncbi Biotransformation of brominated flame retardants into potentially endocrine-disrupting metabolites, with special attention to 2,2',4,4'-tetrabromodiphenyl ether (BDE-47)
    Timo Hamers
    Institute for Environmental Studies IVM, Vrije Universiteit Amsterdam, Amsterdam, The Netherlands
    Mol Nutr Food Res 52:284-98. 2008
    ..In combination with other environmentally relevant hydroxylated organohalogens acting on TTR-binding and E2SULT inhibition, internal exposure to BFR metabolites may significantly contribute to the overall risk of endocrine disruption...
  53. pmc Affinity and location of an internal K+ ion binding site in shaker K channels
    J Thompson
    Department of Pharmacology and Physiology, University of Rochester Medical Center, Rochester, New York 14642, USA
    J Gen Physiol 117:373-84. 2001
    ..These results lead to a refined picture of the number and location of ion binding sites at the inner end of the pore in Shaker K channels...
  54. pmc Competitive binding inhibition enzyme-linked immunosorbent assay that uses the secreted aspartyl proteinase of Candida albicans as an antigenic marker for diagnosis of disseminated candidiasis
    Christine J Morrison
    Mycotic Diseases Branch, Division of Bacterial and Mycotic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    Clin Diagn Lab Immunol 10:835-48. 2003
    ..The potential use of proteinases as markers of invasive candidiasis led us to develop a competitive binding inhibition enzyme-linked immunosorbent assay (ELISA) to detect Sap in clinical specimens...
  55. ncbi Competitive binding of musclin to natriuretic peptide receptor 3 with atrial natriuretic peptide
    Shunbun Kita
    Pharmaceutical Research Division, Pharmacology Research Laboratories I, Takeda Pharmaceutical Company Ltd, Yodogawa ku, Osaka 532 8686, Japan
    J Endocrinol 201:287-95. 2009
    ..We conclude that musclin binds to NPR3 competitively with ANP and may affect ANP concentrations in a local or systemic manner...
  56. ncbi ULBP1, 2, 3: novel MHC class I-related molecules that bind to human cytomegalovirus glycoprotein UL16, activate NK cells
    M Kubin
    Department of Immunobiology, Immunex Corporation, Seattle 98101, USA
    Eur J Immunol 31:1428-37. 2001
    ..Addition of soluble recombinant UL16 protein inhibited the biological activities mediated by ULBP, suggesting the existence of a novel mechanism utilized by CMV to evade elimination by the host immune system...
  57. pmc Streptococcus gordonii Hsa environmentally constrains competitive binding by Streptococcus sanguinis to saliva-coated hydroxyapatite
    Angela H Nobbs
    Department of Diagnostic and Biological Sciences, University of Minnesota, 17 164 Moos Tower, 515 Delaware Street, S E, Minneapolis, MN 55455, USA
    J Bacteriol 189:3106-14. 2007
    ..Next, putative S. gordonii adhesins were analyzed for contributions to interspecies competitive binding. Like wild-type S...
  58. ncbi Competitive binding exchange between alkali metal ions (K+, Rb+, and Cs+) and Na+ ions bound to the dimeric quadruplex [d(G4T4G4)]2: a 23Na and 1H NMR study
    Flaminia Cesare Marincola
    Dipartimento di Scienze Chimiche, Universita di Cagliari, Cittadella Universitaria di Monserrato, S S 554 Bivio per Sestu, I 09042, Monserrato, Cagliari, Italy
    Magn Reson Chem 47:1036-42. 2009
    ....
  59. ncbi Electrochemical survey of the chain length influence in phytochelatins competitive binding by cadmium
    Rui Gusmão
    Departament de Quimica Analitica, Universitat de Barcelona, Spain
    Anal Biochem 406:61-9. 2010
    ..with alternating least squares (MCR-ALS) was applied to voltammetric data obtained in the analysis of the competitive binding of glutathione (GSH) and phytochelatins [(gammaGlu-Cys)(n)-Gly, PC(n), n=2-5] by Cd(2+)...
  60. ncbi Protection against anthrax toxin by recombinant antibody fragments correlates with antigen affinity
    Jennifer A Maynard
    Department of Chemical Engineering, University of Texas, Austin, TX 78712, USA
    Nat Biotechnol 20:597-601. 2002
    ..High-affinity toxin-neutralizing antibodies may be of therapeutic value for alleviating the symptoms of anthrax toxin in infected individuals and for medium-term prophylaxis to infection...
  61. ncbi G protein-coupled receptor allosterism and complexing
    Arthur Christopoulos
    Department of Pharmacology, University of Melbourne, Parkville, Victoria, Australia
    Pharmacol Rev 54:323-74. 2002
    ..It is proposed that the study of allosteric phenomena will become of progressively greater import to the drug discovery process due to the advent of newer and more sensitive GPCR screening technologies...
  62. ncbi Differential effects of heparin saccharides on the formation of specific fibroblast growth factor (FGF) and FGF receptor complexes
    Olga Ostrovsky
    Department of Biology, Technion Israel Institute of Technology, Haifa 32000, Israel
    J Biol Chem 277:2444-53. 2002
    ..These different requirements may reflect the differences in the mode by which a given FGFR interacts with the various FGFs...
  63. ncbi Model of competitive binding of vascular endothelial growth factor and placental growth factor to VEGF receptors on endothelial cells
    Feilim Mac Gabhann
    Department of Biomedical Engineering, School of Medicine, Johns Hopkins University, Traylor 613, 720 Rutland Ave, Baltimore, MD 21205, USA
    Am J Physiol Heart Circ Physiol 286:H153-64. 2004
    ..These results suggest that VEGFR1 signaling can be functional in adult-derived endothelial cells...
  64. ncbi Molecular cloning, pharmacological characterization, and brain mapping of the melanocortin 4 receptor in the goldfish: involvement in the control of food intake
    Jose Miguel Cerdá-Reverter
    Department of Biological Sciences, University of Alberta, Edmonton, Alberta, T6G 2E9 Canada
    Endocrinology 144:2336-49. 2003
    ....
  65. pmc Collaborative competition mechanism for gene activation in vivo
    Joanna A Miller
    Department of Biochemistry, Molecular Biology and Cellular Biology, Northwestern University, Evanston, Illinois 60208 3500, USA
    Mol Cell Biol 23:1623-32. 2003
    ..We speculate that initial target site recognition and binding may occur via spontaneous nucleosomal site exposure, with remodeling factor action required downstream to lock in higher levels of regulatory protein occupancy...
  66. ncbi Attentional effects of nicotinic agonists in rats
    Britta Hahn
    Section of Behavioural Pharmacology, P049, Institute of Psychiatry, King s College London, De Crespigny Park, London, SE5 8AF, UK
    Neuropharmacology 44:1054-67. 2003
    ....
  67. ncbi The breast cell growth inhibitor, estrogen down regulated gene 1, modulates a novel functional interaction between estrogen receptor alpha and transcriptional elongation factor cyclin T1
    Bryan M Wittmann
    Department of Pharmacology, Case Western Reserve University, Cleveland, OH 44106, USA
    Oncogene 24:5576-88. 2005
    ..Conversely, increased EDG1 expression results in inhibition of cyclin T1 recruitment and ERalpha DNA binding. Our results support a novel functional interaction between ERalpha and cyclin T1 that is modulated by EDG1...
  68. ncbi Fibroblast growth factor receptors 1 and 2 interact differently with heparin/heparan sulfate. Implications for dynamic assembly of a ternary signaling complex
    Andrew K Powell
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, United Kingdom
    J Biol Chem 277:28554-63. 2002
    ..We propose a mechanistic model where the kinetic parameters of the HS/FGFR interaction are a key element regulating the formation of ternary complexes and the resulting FGF signaling outcomes...
  69. ncbi Modeling the functional effects of allosteric modulators at pharmacological receptors: an extension of the two-state model of receptor activation
    D A Hall
    Receptor Pharmacology Unit, In Vitro Pharmacology Department, Medicines Research Centre, Glaxo Wellcome Research and Development, Stevenage, Hertfordshire, United Kingdom
    Mol Pharmacol 58:1412-23. 2000
    ....
  70. ncbi The Orf18 gene product from conjugative transposon Tn916 is an ArdA antirestriction protein that inhibits type I DNA restriction-modification systems
    Dimitra Serfiotis-Mitsa
    School of Chemistry, The University of Edinburgh, The King s Buildings, Edinburgh EH9 3JJ, UK
    J Mol Biol 383:970-81. 2008
    ..Our results suggest that ArdA can overcome the restriction barrier following conjugation and so helps increase the spread of antibiotic resistance genes by horizontal gene transfer...
  71. ncbi Cloning and functional expression of the human histamine H3 receptor
    T W Lovenberg
    R W Johnson Pharmaceutical Research Institute, San Diego, California, USA
    Mol Pharmacol 55:1101-7. 1999
    ..The availability of the human H3 receptor cDNA should greatly aid in the development of chemical and biological reagents, allowing a greater appreciation of the role of histamine in brain function...
  72. ncbi Pharmacological comparison of rat and human melanocortin 3 and 4 receptors in vitro
    Helgi B Schioth
    Department of Neuroscience, Biomedical Center, Box 593, 751 24 Uppsala University, Uppsala, Sweden
    Regul Pept 106:7-12. 2002
    ..Pharmacological species difference have earlier been reported for the MC1 and MC5 receptors but this is the first report showing important differences between the rat and human MC4 receptors...
  73. ncbi Competitive binding of postsynaptic density 95 and Ca2+-calmodulin dependent protein kinase II to N-methyl-D-aspartate receptor subunit 2B in rat brain
    Fan Jie Meng
    Research Center of Biochemistry and Molecular Biology, Xuzhou Medical College, Xuzhou 221002, China
    Acta Pharmacol Sin 25:176-80. 2004
    ....
  74. ncbi Lysine 58 and histidine 66 at the C-terminal alpha-helix of monocyte chemoattractant protein-1 are essential for glycosaminoglycan binding
    L Chakravarty
    Neurobiotechnology Center and the Departments of Biochemistry and Medical Biochemistry, The Ohio State University, Columbus, Ohio 43210, USA
    J Biol Chem 273:29641-7. 1998
    ..Therefore, we conclude that the Lys-58 and His-66 residues in the C-terminal alpha-helix of MCP-1 are essential for glycosaminoglycan binding and probably for the binding to the endothelial surface proteoglycans...
  75. ncbi Inhibition of the transcription of CYP1A1 gene by the upstream stimulatory factor 1 in rabbits. Competitive binding of USF1 with AhR.Arnt complex
    Y Takahashi
    Division of Drug Metabolism, Faculty of Pharmaceutical Sciences, Hokkaido University, N12W6, Kita ku, Sapporo, Hokkaido 060, Japan
    J Biol Chem 272:30025-31. 1997
    ..These results indicate that the XRE of the rabbit CYP1A1 gene is recognized by the basic helix-loop-helix proteins to regulate the expression of CYP1A1 in both an agonistic (AhR.Arnt) and an antagonistic (USF1) manner...
  76. pmc The E-SCREEN assay as a tool to identify estrogens: an update on estrogenic environmental pollutants
    A M Soto
    Tufts University School of Medicine, Department of Anatomy and Cellular Biology, Boston, MA 02111, USA
    Environ Health Perspect 103:113-22. 1995
    ....
  77. ncbi Structure-function analysis of a phage display-derived peptide that binds to insulin-like growth factor binding protein 1
    N J Skelton
    Department of Protein Engineering, Genentech, Inc, 1 DNA Way, South San Francisco, California 94080, USA
    Biochemistry 40:8487-98. 2001
    ..By comparison of alanine scanning data for IGF-I and the turn-helix peptide, we propose a model for common surface features of these molecules that recognize IGFBP-1...
  78. ncbi Intron-mediated expression of the human neuropeptide Y Y1 receptor
    Ulrica Marklund
    Department of Molecular Biology, AstraZeneca R and D, Tvistevägen 48, S 907 36 Umeå, Sweden
    Mol Cell Endocrinol 188:85-97. 2002
    ....
  79. ncbi The CXC chemokine SDF-1 is the ligand for LESTR/fusin and prevents infection by T-cell-line-adapted HIV-1
    E Oberlin
    Unite d Immunologie Virale, Institut Pasteur, Paris, France
    Nature 382:833-5. 1996
    ....
  80. ncbi Pesticides interfere with the nongenomic action of a progestogen on meiotic maturation by binding to its plasma membrane receptor on fish oocytes
    S Das
    Department of Internal Medicine, Yale University, School of Medicine, VA Medical Center, West Haven, CT 06516, USA
    Endocrinology 140:1953-6. 1999
    ..The close correspondence between competitive binding of the two pesticides to the 20beta-S membrane receptor and their inhibition of 20beta-S induced FOM ..
  81. ncbi Receptor-mediated effects on ligand availability influence relative mitogenic potencies of epidermal growth factor and transforming growth factor alpha
    C C Reddy
    Department of Chemical Engineering, University of Illinois at Urbana Champaign, Urbana, Illinois 61801, USA
    J Cell Physiol 166:512-22. 1996
    ..Our results suggest a model of regulation of hormone responsiveness which favors dissociative ligands (such as TGF alpha) in receptor-limited situations and non-dissociative ligands (such as EGF) in the face of high receptor levels...
  82. ncbi PKCepsilon activation induces dichotomous cardiac phenotypes and modulates PKCepsilon-RACK interactions and RACK expression
    J M Pass
    Department of Physiology and Biophysics, University of Louisville, Louisville, Kentucky 40292, USA
    Am J Physiol Heart Circ Physiol 280:H946-55. 2001
    ..Our observations demonstrate that RACK expression is dynamically regulated by PKCepsilon and suggest that differential patterns of PKCepsilon-RACK interactions may be important determinants of PKCepsilon-dependent cardiac phenotypes...
  83. ncbi Stimulation of aromatase P450 promoter (II) activity in endometriosis and its inhibition in endometrium are regulated by competitive binding of steroidogenic factor-1 and chicken ovalbumin upstream promoter transcription factor to the same cis-acting elem
    K Zeitoun
    Cecil H Ida Green Center for Reproductive Biology Sciences, Department of Obstetrics and Gynecology, The University of Texas Southwestern Medical Center, Dallas 75235 9051, USA
    Mol Endocrinol 13:239-53. 1999
    ....
  84. ncbi Nicotine upregulates its own receptors through enhanced intracellular maturation
    Jérôme Sallette
    CNRS URA D2182 Récepteurs et Cognition, Institut Pasteur, Paris, France
    Neuron 46:595-607. 2005
    ....
  85. pmc Cloning of the human eosinophil chemoattractant, eotaxin. Expression, receptor binding, and functional properties suggest a mechanism for the selective recruitment of eosinophils
    P D Ponath
    LeukoSite, Inc, Cambridge, Massachusetts 02142, USA
    J Clin Invest 97:604-12. 1996
    ..Thus eotaxin in humans is a potent and selective eosinophil chemoattractant that is expressed by a variety cell types in certain inflammatory conditions...
  86. ncbi Potent competitive interactions of some brominated flame retardants and related compounds with human transthyretin in vitro
    I A Meerts
    Toxicology Group, Department of Food Technology and Nutritional Sciences, Wageningen University and Research Center, Tuinlaan 5, 6703 HE Wageningen, The Netherlands
    Toxicol Sci 56:95-104. 2000
    ..the possible interaction of several brominated flame retardants with T(4) binding to TTR in an in vitro competitive binding assay, using human TTR and 125 I-T(4) as the displaceable radioligand...
  87. ncbi The Sp1-like protein BTEB3 inhibits transcription via the basic transcription element box by interacting with mSin3A and HDAC-1 co-repressors and competing with Sp1
    J Kaczynski
    Gastroenterology Research Unit, Mayo Clinic, Rochester, Minnesota 55901, USA
    J Biol Chem 276:36749-56. 2001
    ..Furthermore, our results suggest a mechanism of repression for BTEB3 involving direct repression by the N terminus via interaction with mSin3A and HDAC-1 and competition with Sp1 via the DNA-binding domain...
  88. ncbi Molecular modeling of wild-type and D816V c-Kit inhibition based on ATP-competitive binding of ellipticine derivatives to tyrosine kinases
    Jeremie Vendome
    CNRS UMR 8113, Laboratoire de Biotechnologies et Pharmacologie génétique Appliquée LBPA Ecole Normale Supérieure de Cachan, 61 Avenue du President Wilson, 94235 Cachan, France
    J Med Chem 48:6194-201. 2005
    ..The model provides possible explanations for the structure-activity relationships observed among ellipticine compounds, resulting in new insights into D816V c-Kit mutant inhibition...
  89. ncbi The behavior of mixtures of paralytic shellfish toxins in competitive binding assays
    Lyndon E Llewellyn
    Australian Institute of Marine Science, PMB 3, Townsville MC, Queensland, 4810, Australia
    Chem Res Toxicol 19:661-7. 2006
    ..This equation has been extended to mixtures of PSTs containing more than three toxins and may be applicable to other natural contaminants and any competitive binding assays used to detect their presence and measure their concentration.
  90. ncbi A fluorescent intercalator displacement assay for establishing DNA binding selectivity and affinity
    Winston C Tse
    Department of Chemistry and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Acc Chem Res 37:61-9. 2004
    ....
  91. ncbi Development of a competitive binding assay system with recombinant estrogen receptors from multiple species
    Cynthia V Rider
    Department of Molecular Biomedical Sciences, North Carolina State University, Raleigh, NC 27606, USA
    Toxicol Lett 184:85-9. 2009
    ..This system provides a convenient in vitro approach for directly comparing chemical binding to estrogen receptors across multiple species without the need to sacrifice animals...
  92. ncbi Cooperative and competitive binding in synergistic mixtures of Thermobifida fusca cellulases Cel5A, Cel6B, and Cel9A
    Tina Jeoh
    Department of Biological and Environmental Engineering and Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14853, USA
    Biotechnol Prog 18:760-9. 2002
    ....
  93. pmc External TEA block of shaker K+ channels is coupled to the movement of K+ ions within the selectivity filter
    Jill Thompson
    Department of Pharmacology and Physiology, University of Rochester Medical Center, Rochester, NY 14642, USA
    J Gen Physiol 122:239-46. 2003
    ....
  94. pmc Binding of the G domains of laminin alpha1 and alpha2 chains and perlecan to heparin, sulfatides, alpha-dystroglycan and several extracellular matrix proteins
    J F Talts
    Max Planck Institut fur Biochemie, Am Klopferspitz 18a, D 82152 Martinsried, Germany
    EMBO J 18:863-70. 1999
    ..This demonstrated similar binding repertoires for the LG modules of three basement membrane proteins involved in cell-matrix interactions and supramolecular assembly...
  95. ncbi Antiobesity efficacy of a novel cannabinoid-1 receptor inverse agonist, N-[(1S,2S)-3-(4-chlorophenyl)-2-(3-cyanophenyl)-1-methylpropyl]-2-methyl-2-[[5-(trifluoromethyl)pyridin-2-yl]oxy]propanamide (MK-0364), in rodents
    Tung M Fong
    Department of Metabolic Disorders, Merck Research Laboratories, R80M 213, P O Box 2000, Rahway, NJ 07065, USA
    J Pharmacol Exp Ther 321:1013-22. 2007
    ..These studies demonstrated that MK-0364 is a highly potent and selective CB1R inverse agonist and that it is orally active in rodent models of obesity...
  96. ncbi Saturated fat-induced changes in Sf 60-400 particle composition reduces uptake of LDL by HepG2 cells
    Kim G Jackson
    School of Food Biosciences, University of Reading, Reading, Berkshire, United Kingdom
    J Lipid Res 47:393-403. 2006
    ..008). In conclusion, these findings suggest an alternative or additional mechanism whereby acute fat ingestion can influence LDL clearance via competitive apoE-dependent effects of TRL on the LDL receptor...
  97. ncbi Glucose sensors based on microcapsules containing an orange/red competitive binding resonance energy transfer assay
    Swetha Chinnayelka
    Biomedica Engineering Program, Institute for Micromanufacturing, Louisiana Tech University, Ruston, 71272, USA
    Diabetes Technol Ther 8:269-78. 2006
    ..A recent development towards this goal is a highly specific and sensitive competitive binding assay for glucose using apo-glucose oxidase (apo-GOx) as the recognition element and dextran as the ..
  98. pmc Maintenance of antigen-specific immunological memory through variable regions of heavy and light chains of anti-idiotypic antibody
    J Vani
    Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, Karnataka, India
    Immunology 120:486-96. 2007
    ..Evidence has been provided for the existence of an antigen-specific B-cell idiotypic network in the body that supports the perpetuation of immunological memory as proposed in the relay hypothesis...
  99. ncbi The anomalous behaviour of exogenous 25-hydroxyvitamin D in competitive binding assays
    G D Carter
    Department of Clinical Chemistry, Hammersmith Hospitals NHS Trust, London W6 8RF, UK
    J Steroid Biochem Mol Biol 103:480-2. 2007
    ..9, 46.4%, 43.2%, HPLC (n=9); 42.6, 112.2%, 97.1%, LC-MS (n=4); 34.0, 111.5%, 118.1%. The IDS RIA and Nichols assays gave unexpectedly low recoveries. This does not appear to be a calibration problem or the effect of ethanol...
  100. pmc Evaluation of novel antimouse VEGFR2 antibodies as potential antiangiogenic or vascular targeting agents for tumor therapy
    Sophia Ran
    Simmons Comprehensive Cancer Center, University of Texas Southwestern Medical Center, Dallas, TX 75390 8594, USA
    Neoplasia 5:297-307. 2003
    ..Vascular damage was not observed in normal organs, including kidneys and pancreas. These studies demonstrate that anti-VEGFR2 antibodies have potential for vascular targeting and imaging of tumors in vivo...
  101. ncbi Host-guest chemistry of dendrimer-drug complexes. 3. Competitive binding of multiple drugs by a single dendrimer for combination therapy
    Libo Zhao
    Laboratory of Functional Membranes, Department of Chemistry, University of Science and Technology of China, Hefei, Anhui 230026, PR China
    J Phys Chem B 113:14172-9. 2009
    ..The competitive binding of multiple drugs by a single dendrimer in aqueous solutions was investigated by (1)H NMR and 2D-NOESY ..

Research Grants63

  1. Novel genomic effects of Y-linked polymorphisms
    Daniel L Hartl; Fiscal Year: 2012
    ..population dynamics of polymorphic Y chromosomes associated with YRV to determine whether a model based on competitive binding is possible with realistic values of the parameters...
  2. Dual Direct Inhibitors of Thrombin and Factor Xa
    UMESH RAMANLAL DESAI; Fiscal Year: 2012
    ..7) Competitive binding studies suggest that CDs interacts with exosite II of thrombin, a site not typically associated with ..
  3. Structure and function analysis of C1/C1q and MBL using a novel peptide inhibitor
    NEEL KUMAR KRISHNA; Fiscal Year: 2012
    ..which CPP interaction with C1q alters interaction with its cognate serine protease complex by utilizing a competitive binding assay developed in our laboratory (ELISA)...
  4. High throughput assay for fibrin-binding probes
    PETER D CARAVAN; Fiscal Year: 2013
    ..We have shown that these peptides also bind to a soluble fibrin degradation product, DD(E), and that a competitive binding assay can be implemented...
  5. FACTOR VA INTERACTIONS REGULATING PROTHROMBIN ACTIVATION
    Paul E Bock; Fiscal Year: 2010
    ..inactivation by APC through ordered cleavages at Arg506, Arg306, and Arg679 is achieved by exosite-mediated competitive binding of factor Xa and APC to factor Va for cleavage at Arg506, and specific acceleration of cleavage at Arg306 ..
  6. Corneal Expression and Function of Slurp1
    SHIVALINGAPPA KOTTUR SWAMYNATHAN; Fiscal Year: 2013
    ..keratitis and (b) Slurp1 bound the uPAR ligand uPA, suggesting that Slurp1 inhibits inflammation by competitive binding of UPAR ligands. We will test our hypothesis by pursuing three Specific Aims...
  7. The role of IL-7 in guiding developing B cells through the pre-B cell check-point
    Kristen Johnson; Fiscal Year: 2010
    ..Specifically, I will explore mechanisms that include competitive binding and altered locus accessibility considering both localized changes in chromatin structure as well as more ..
  8. The role of HDAC2 in hormone therapy resistance
    Pamela N Munster; Fiscal Year: 2013
    ..The two most commonly used methods of ER inhibition are either competitive binding of the ER with anti-estrogens (e.g...
  9. Anti-inflammatory and Mitotic Properties of Non-hormonal Steroids in Lung Epithel
    ERICA REEVES; Fiscal Year: 2010
    ..The VBP series shows lack of competitive binding to the glucocorticoid receptor, yet retains strong transrepression (signaling) activities of ..
  10. Development of a computational model for improved diagnostic accuracy of DNA micr
    David A Stahl; Fiscal Year: 2010
    ..mathematical model of microarray hybridization that captures the essential features of our platform (competitive binding and dissociation in three-dimensional gel elements, diffusion and convective flow, effects of target length,..
  11. IMAGING AGENTS FOR BETA CELL MASS OF PANCREAS
    Hank F Kung; Fiscal Year: 2013
    ..and the uptake was blocked (>80%) by a pretreatment of a challenging dose of VMAT2 ligand, suggesting a competitive binding to the same VMAT2 binding sites...
  12. Novel targets for discovering peptide inhibitors of HIV-1 replication
    Xinhong Dong; Fiscal Year: 2011
    ..Screen for peptide candidates against libraries will be performed using direct binding and competitive binding inhibition assays. The binding dynamics of peptide candidates with target proteins will be characterized...
  13. Multi-User, Isothermal Titration Microcalorimeter
    IVAN JULIAN DMOCHOWSKI; Fiscal Year: 2013
    ..directly sub-millimolar to nanomolar binding constants~ stronger interactions can be investigated via competitive binding. Technical advances over the previous model enable this instrument to measure samples of roughly 7-fold ..
  14. Development of Translation Initiation Inhibitors and their Anti-Cancer Activity
    Michael Chorev; Fiscal Year: 2012
    ..b>competitive binding to eIF4E, 2. inhibition of cancer cell growth, 3...
  15. Drug Screening with Nano-Porous Silicon Optical Biosensors
    LISA BONANNO; Fiscal Year: 2010
    ..The first aim will focus on developing a semi-quantitative screening assay in PSi. A competitive binding assay will target opiates in urine as a proof of concept...
  16. Development of High Throughput Aptamer-based Protein Capture/Detection Assays tow
    John T Lis; Fiscal Year: 2013
    ..After validation of non-competitive binding of a pair of aptamer to individual target proteins, these aptamer pairs will be utilized in high throughput ..
  17. Biotransformation of Estrogens to Carcinogenic Quinoids
    JUDY BOLTON; Fiscal Year: 2005
    ..The relative binding affinity of these compounds to ERa and ERb proteins will be measured using a competitive binding assay...
  18. Bivalent ligands as molecular probes for CB1/OX1 receptor heterodimers
    Yanan Zhang; Fiscal Year: 2010
    ..All the compounds will be tested in competitive binding assays using single transfected cell lines, expressing either CB1 or OX1 receptors...
  19. SOMATOSTATIN RECEPTORS--CLASSIFICATION OF NEUROBLASTOMAS
    M O DORISIO; Fiscal Year: 1999
    ..b>Competitive binding techniques will be used to identify high affinity binding sites as a measure of functional receptor protein...
  20. INTESTINAL & PLACENTAL FC-RECEPTORS FOR IMMUNOGLOBULIN
    Neil Simister; Fiscal Year: 2000
    ..The specificity of human FcRn for different human IgG subclasses, measured by a competitive binding assay, will be compared with the relative efficiency of placental transport of these subclasses...
  21. Development of Platform for Ligand Screening using Hyperpolarized NMR
    CHRISTIAN B HILTY; Fiscal Year: 2013
    ..Furthermore, other drug molecules can be identified by competitive binding to a target site together with a fluorinated reporter ligand...
  22. Discovery of A-beta Oligomer Antagonists via 3D Pharmacophore Space Analysis
    CHRISTIAN ALEXANDER LANG; Fiscal Year: 2012
    ..The second step consists of biochemical assays including competitive binding with bexarotene, and ApoE level determination in cultured mouse brain cells...
  23. Mechanism of Neuropilin Dependent Angiogenesis
    CRAIG VANDER KOOI; Fiscal Year: 2013
    ..In Aim 1, we will determine the basis for competitive binding of ligands to neuropilin, and the role that this competition plays in regulating angiogenesis...
  24. Complementation assay for protein kinase inhibitors
    RICHARD EGLEN; Fiscal Year: 2002
    ..The complementation reaction is used as a signaling system for competitive binding assays and has been adapted by us to measure reactions such as ligand binding to receptors, enzymatic ..
  25. Y Receptor In and Cardiovascular Function From the PHN
    John Martin; Fiscal Year: 2004
    ..IP3 levels will be measured using competitive binding between [3H]IP3 and IP3 from the tissue slices to a binding protein...
  26. MOLECULAR BASIS OF COAGULATION PROTEASE SPECIFICITY
    Alireza R Rezaie; Fiscal Year: 2010
    ..These studies will utilize fluorescence spectroscopy, enzyme kinetics, and competitive binding methods to provide critical information about the kinetic and thermodynamic constants that define the ..
  27. GLIAL B-AMYLOID PEPTIDE RECEPTORS IN ALZHEIMER'S DISEASE
    Kurt Brunden; Fiscal Year: 1993
    ..Dr. Brunden found that 125I-substance P binds specifically to these glial cells. Using competitive binding assays he also showed that this binding was inhibited by the amino acid residues BAP (1-40) and BAP (25-35),..
  28. BAND 3 N-TERMINAL AA (10-16) AND DEOXY S POLYMERIZATION
    John Harris; Fiscal Year: 1990
    ..of reactions with hemoglobin will be by Csat, time-viscosity profile, physical properties of gel, P50, competitive binding with other polyanions, change in charge and M.W...
  29. Autoregulated insulin delivery particles for the treatment of diabetes will be de
    Mostafa Analoui; Fiscal Year: 2009
    ..The major shortcoming of the current AVT however, is that the glucose-sensitive linkage is based on competitive binding of concanavalin-A (Con-A) to a sugar...
  30. ORAL ECOLOGY OF PROSTHETIC SURFACES
    Mira Edgerton; Fiscal Year: 1991
    ..albicans adherence to acrylic surfaces is DIS will be done. This will be accomplished by competitive binding studies between C...
  31. DYNAMICS OF MICROTUBULE ASSEMBLY/DISASSEMBLY
    JOHN CORREIA; Fiscal Year: 1993
    ..Therefore, competitive binding studies will be undertaken in the presence of other metals, especially Mn2+ and Al3+, and over a wide range ..
  32. Anthracycline cardiotoxicity and calsequestrin function
    SUSAN SHADLE; Fiscal Year: 2002
    ..3) to probe the ability of anthracyclines to inhibit calcium binding to calsequestrin using Ca-45 competitive binding assays; 4) to determine the conformation of the cardiac ryanodine receptor (RyR2) in isolated SR vesicles ..
  33. BROAD NEUTRALIZING RESPONSE TO HIV/VEE REPLICONS
    Gerald Quinnan; Fiscal Year: 1999
    ..The epitopes responsible for the cross-reactivity of R2 will be sought by competitive binding studies using sera with NA which do and do not neutralize R2 and monoclonal antibodies which are currently ..
  34. REPRESSION OF T CELL ACTIVATION BY ENDOCANNABINOIDS
    Norbert Kaminski; Fiscal Year: 2003
    ..To date a number of putative eicosinoid-like cannabinoids have been identified by competitive binding as endogenous cannabinoid receptor ligands...
  35. RELATIONSHIPS BETWEEN PROSTANOIDS AND CELLULAR INJURY
    John Flynn; Fiscal Year: 2000
    ..Equilibrium and kinetic receptor binding, (125)I-LPS competitive binding, receptor occupancy and receptor affinity studies will be used to characterize the nature of the receptor...
  36. RADIOPHARMACEUTICALS TARGETED TO TUMOR FOLATE RECEPTORS
    Mark Green; Fiscal Year: 2003
    ..The affinity of the conjugate for cell uptake via the folate receptor will be determined in vitro through competitive binding assays with cultured human tumor cells...
  37. Hypocretins and Their Role in the Control of Sleep
    RICHARD MAKI; Fiscal Year: 2002
    ..The antagonists will be tested in competitive binding assays and cell-based functional assays. Second, the small molecule antagonists will be tested in vivo...
  38. MOLECULAR MECHANISM OF BREVETOXIN AND CIGUATOXIN INSULT
    Daniel Baden; Fiscal Year: 2000
    ..Evaluate the effects of toxin derivatization on binding of [3H] brevetoxin PbTx-3 to ensure and quantify competitive binding and to ensure high affinity...
  39. ROLE OF INSULIN-LIKE GROWTH FACTORS IN THE PNS
    Eva Feldman; Fiscal Year: 1993
    ..sites will be determined in a model system, the SH-SY5Y neuroblastoma clone, using iodinated ligands, competitive binding curves and affinity crosslinking...
  40. Novel calpain inhibitors based on phage display
    RODNEY GUTTMANN; Fiscal Year: 2005
    ..BIAcore technology will be used in addition to Elisa based assays to assay for calcium-dependent competitive binding between calpastatin, tau and LSEAL. We will also test additional calpain substrates...
  41. MOLECULAR COEVOLUTION OF GROWTH HORMONE AND ITS RECEPTOR
    Wen Hsiung Li; Fiscal Year: 2002
    ..5. Use the variant GHs and GHBPs in competitive binding assays to determine the effect of each change...
  42. STUDIES OF DIFFERENTIALLY EXPRESSED LEISHMANIA ANTIGENS
    DIANE MCMAHON PRATT; Fiscal Year: 2003
    ..The CD1d -P-8 glycolipid(s) interaction will be investigated and KB determined using competitive binding and/or BIAcor methods...
  43. COMPLEMENTATION ASSAY TO SCREEN FOR KINASE INHIBITORS
    RICHARD EGLEN; Fiscal Year: 2004
    ..protein kinase assay which employs our proprietary CEDIA(r) technology and consists of measuring the competitive binding of staurosporine, a universal protein kinase ligand, to individual recombinant protein kinases...
  44. IMPAIRED SOMATOMEDIN RESPONSIVENESS AND GROWTH FAILURE
    Stephen Rosenthal; Fiscal Year: 1990
    ..Previous attempts to identify children with IGF-I receptor defects have utilized competitive binding studies and measurements of receptor responsiveness, and suggest that IGF-I receptor defects constitute a ..
  45. COMPARATIVE STUDIES OF SOMATOMEDIN AND INSULIN RECEPTORS
    Ron Rosenfeld; Fiscal Year: 1991
    ..These antibodies will be employed, together with competitive binding and affinity cross-linking, to determine tissue and species specificity of the receptors, ontogeny of each ..
  46. INVESTIGATION OF THE BLOCK TO POLYSPERMY IN A. CERATODES
    CHRISTINA GOODE; Fiscal Year: 2001
    ..and follicle cells will be isolated and characterized using kinetic analysis, physical characterization and competitive binding assays. In addition, Dr...
  47. MONO-SUBSTITUTED CONJUGATES IN ENZYMEIMMUNOASSAYS
    LEONIDAS BACHAS; Fiscal Year: 1992
    Enzyme-linked competitive binding assays are well recognized as alternatives to radioimmunoassays for the sensitive and selective determination of a variety of biologically important analytes (ligands)...
  48. GTP BINDING PROTEINS IN MITOCHONDRIAL PROTEIN IMPORT
    RONALD PAYNE; Fiscal Year: 2001
    ..Mutations in the transit peptide, competitive binding assays, and protease protection assays will identify the components of ribosome-mitochondrial binding...
  49. Molecular Regulation of TIMP-2 in Neuron Cell Cycle
    DIANE JAWORSKI; Fiscal Year: 2007
    ..In aim 2, the integrin(s) to which TIMP-2 binds will be identified and competitive binding assays will be performed to block the phenotypic rescue by TIMP-2...
  50. Identify BCL-2 "gain of function"inhibitors (RMI)
    Michael Manion; Fiscal Year: 2004
    ..The counter-screen will involve a competitive binding assay based on the anisotropy changes measured when AA binds to BCL-XL...
  51. AGONISTS/ANTAGONISTS FOR CHARACTERIZING CHEMORECEPTORS
    Bruce Bryant; Fiscal Year: 1993
    ..using both stimulatory effectiveness and cross-adaptation procedures in a neurophysiological assay and competitive binding in biochemical assays. The resultant characterized probes and competitive binding in biochemical assays...
  52. QUANTITATIVE TEST FOR THEOPHYLLINE IN SALIVA
    James Johnston; Fiscal Year: 1993
    ..Like EMIT assays, the device will utilize competitive binding of theophylline and an analyte-enzyme conjugate to a monoclonal anti-theophylline antibody...
  53. Small animal fluorescence enhanced optical tomography
    Eva Sevick Muraca; Fiscal Year: 2006
    ..specificity of imaging agents c(KRGDf)-IRDye800, 1111n-c(KRGDf), and 1111n- c(KRDGf)-IRDye800 through competitive binding and determination of IC50 in cell cultures of integrin positive M21 and integrin negative M21-L; 2...
  54. Highly Multiplexed, SERS-Based Quantitation Nanotags
    Richard Freeman; Fiscal Year: 2004
    ..Finally, we will carry out a competitive binding assay between biotin and desthiobiotin to show that the SERS nanotags can provide quantitative data in a ..
  55. AEQUORIN AS A LABEL IN BIOLUMINESCENCE IMMUNOASSAYS
    Sylvia Daunert; Fiscal Year: 1999
    ..In this study, we propose to develop and evaluate novel competitive binding assays for biomolecules by taking advantage of the selective interaction between biological binders (..
  56. Small Molecule Inhibitors of BcI xL Survival Protein
    David Hockenbery; Fiscal Year: 2004
    ..We confirmed this interaction by showing competitive binding of AA and its 2-methoxy derivative with a known hydrophobic groove ligand to recombinant Bcl-xL and Bcl-2 ..
  57. PROTEINS IN MOLECULAR MECHANISMS OF TEAR FILM FORMATION
    BEN GLASGOW; Fiscal Year: 2000
    ..A. Measurement of the binding affinity of lipocalins for various lipid ligands. Competitive binding studies will be performed with an array of lipid compounds found in native tears...
  58. Evolving Libraries of Bivalent Compounds (RMI)
    Kevin Burgess; Fiscal Year: 2007
    ..it possible to screen the libraries via direct binding assays; these have considerable advantages over the competitive binding assays that are often used for protein-protein interaction targets...
  59. Cholesterol Trafficking by Human Serum Albumin
    CHRISTOPHER ABELT; Fiscal Year: 2007
    ..b>Competitive binding studies with well known HSA ligands will establish the position of the binding sites and reveal allosteric ..
  60. HEPATIC DIRECTED ANTIVIRAL AGENTS
    Philip Enriquez; Fiscal Year: 1992
    ..Interaction with the hepatic asialoglycoprotein receptor in vitro will be determined in a competitive binding assay using isolated rat hepatocytes...
  61. KIDNEY DERIVED SOMATOSTATIN
    MARTIN TURMAN; Fiscal Year: 2002
    ..and production of FN and TGF-beta1, and b) testing for sst subtype expression by Northern, immunoblot, and competitive binding analysis...
  62. Computer Modeling of Perlecan as a Vascular Regulator
    MICHAEL W FANNON; Fiscal Year: 2010
    ..Test the model using competitive binding assays with growth factors alone or in combination with other factors as a function of perlecan ..
  63. IMMUNOCHROMATOGRAPHY ASSAY FOR MICROALBUMINURIA
    ROBERT CARRICO; Fiscal Year: 1992
    ..The assay is based on immunochromatography with urine as the developing liquid. A competitive binding immunoassay format will be evaluated...