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| amnionSummarySummary: The innermost membranous sac that surrounds and protects the developing EMBRYO which is bathed in the AMNIOTIC FLUID. Amnion cells are secretory EPITHELIAL CELLS and contribute to the amniotic fluid. Top Publications
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Publications
Concise review: isolation and characterization of cells from human term placenta: outcome of the first international Workshop on Placenta Derived Stem CellsOrnella Parolini
Centro di Ricerca E Menni, Fondazione Poliambulanza, Istituto Ospedaliero, Via Bissolati 57, 25124 Brescia, Italy
Stem Cells 26:300-11. 2008....
Engraftment potential of human amnion and chorion cells derived from term placentaMarco Bailo
Centro Ricerche Parco Scientifico E. Menni, Ospedale Poliambulanza, Via Romiglia, 4, I-25124 Brescia, Italy
Transplantation 78:1439-48. 2004..which may entail progenitor potential. The immunologic reactivity and the transplantation potential of amnion and chorion cells, however, remain to be elucidated...
Stem cell characteristics of amniotic epithelial cellsToshio Miki
Department of Pathology and McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA
Stem Cells 23:1549-59. 2005..b>Amnion derived from term placenta after live birth may be a useful and noncontroversial source of stem cells for cell ..
Human amnion epithelial cell transplantation abrogates lung fibrosis and augments repairYuben Moodley
F R A C P School of Medicine and Pharmacology, University of Western Australia, 50 Murray Street, Perth, Western Australia 6000, Australia
Am J Respir Crit Care Med 182:643-51. 2010..Cellular therapies that could restore pneumocytes and reduce inflammation and fibrosis would be a major advance in management...
Immunosuppressive factors secreted by human amniotic epithelial cellsHaochuan Li
Department of Ophthalmology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
Invest Ophthalmol Vis Sci 46:900-7. 2005..Moreover, AEC supernatant inhibited macrophage migration in vitro. CONCLUSIONS: AECs secrete soluble factors that inhibit cells in both the innate and adaptive immune systems...
Stem cells derived from human fetal membranes display multilineage differentiation potentialSivakami Ilancheran
Center for Women s Health Research, Department of Obstetrics and Gynecology, Monash University, Monash Medical Center, Clayton, Victoria 3168, Australia
Biol Reprod 77:577-88. 2007The amnion is the inner of two membranes surrounding the fetus. That it arises from embryonic epiblast cells prior to gastrulation suggests that it may retain a reservoir of stem cells throughout pregnancy...
Transplantation of allogeneic and xenogeneic placenta-derived cells reduces bleomycin-induced lung fibrosisAnna Cargnoni
Centro di Ricerca E Menni, Fondazione Poliambulanza Istituto Ospedaliero, 25124 Brescia, Italy
Cell Transplant 18:405-22. 2009Fetal membranes (amnion and chorion) have recently raised significant attention as potential sources of stem cells...
Dose-dependent immunomodulatory effect of human stem cells from amniotic membrane: a comparison with human mesenchymal stem cells from adipose tissueSusanne Wolbank
Red Cross Blood Transfusion Service of Upper Austria, Linz, Austria
Tissue Eng 13:1173-83. 2007..All three cell types may be considered as possible alternatives to BMSCs for allogeneic application in tissue engineering...
Isolation, characterization, and differentiation of stem cells derived from the rat amniotic membraneAkiva J Marcus
The Ira B Black Center for Stem Cell Research, Department of Neuroscience and Cell Biology, University of Medicine and Dentistry of New Jersey Robert Wood Johnson Medical School, Piscataway, NJ 08854 5635, USA
Differentiation 76:130-44. 2008..Similar to mesenchymal stem cells, these amnion-derived stem cells (ADSCs) express the surface markers CD29 and CD90, but were negative for the ..
Comparative characterization of cultured human term amnion epithelial and mesenchymal stromal cells for application in cell therapyGrozdana Bilic
Department of Obstetrics, University Hospital Zurich, Zurich, Switzerland
Cell Transplant 17:955-68. 2008Emerging evidence suggests human amnion tissue as a valuable source of two distinct types of pluripotent cells, amnion epithelial cells (hAECs) and mesenchymal stromal cells (hAMSCs), for applications in cell replacement therapy...
Human amniotic mesenchymal cells have some characteristics of cardiomyocytesPeng Zhao
Department of Organ Regeneration, Institute of Organ Transplants, Reconstructive Medicine and Tissue Engineering, Shinshu University School of Medicine, Matsumoto, Japan
Transplantation 79:528-35. 2005..CONCLUSION: The results of the present study suggest that hAMC possess some characteristics of cardiomyocytes...
Transplantation of human amnion epithelial cells reduces hepatic fibrosis in immunocompetent CCl₄-treated miceUrsula Manuelpillai
Centre for Reproduction and Development, Monash Institute of Medical Research, Monash University, Clayton, Victoria, Australia
Cell Transplant 19:1157-68. 2010..To test this hypothesis we transplanted human amnion epithelial cells (hAEC), isolated from term delivered placenta, into immunocompetent C57/BL6 mice at week 2 of a 4-..
Epidermal growth factor and interleukin-1beta utilize divergent signaling pathways to synergistically upregulate cyclooxygenase-2 gene expression in human amnion-derived WISH cellsWilliam E Ackerman
Department of Obstetrics and Gynecology Division of Maternal Fetal Medicine and Laboratory of Perinatal Research, The Ohio State University, College of Medicine and Public Health, Columbus, Ohio 43210, USA
Biol Reprod 71:2079-86. 2004..It was previously demonstrated that EGF could potentiate IL-1beta-driven PGE(2) production in amnion and amnion-derived (WISH) cells...
Identification of stem cell marker-positive cells by immunofluorescence in term human amnionToshio Miki
Department of Pathology, University of Pittsburgh, Pittsburgh, PA 15213, USA
J Reprod Immunol 75:91-6. 2007..Stem cell marker-positive cells were found in amnion but not in choriodecidua, tissues known to contain hematopoietic and trophoblastic stem cells...
Factors secreted by human amniotic epithelial cells promote the survival of rat retinal ganglion cellsSaiko Uchida
Department of Ophthalmology, Tokyo University School of Medicine, Tokyo 113-8655, Japan
Neurosci Lett 341:1-4. 2003..These results suggest that HAECs may produce unknown neuroprotective factors, suggesting its potential for the treatment of RGC degenerative diseases...
Amniotic membrane and amniotic cells: potential therapeutic tools to combat tissue inflammation and fibrosis?U Manuelpillai
Monash Institute of Medical Research, Monash University, Clayton, Victoria 3168, Australia
Placenta 32:S320-5. 2011....
Role of human amniotic epithelial cell transplantation in spinal cord injury repair researchV Sankar
Department of Anatomy, Dr Arcot Lakshmanaswamy Mudaliar Postgraduate Institute of Basic Medical Sciences, Taramani Campus, University of Madras, 600 113, Chennai, India
Neuroscience 118:11-7. 2003..First, there was no evidence of immunological rejection probably due to the non-antigenic nature of the HAEC. Second, unlike neural tissue, procurement of HAEC does not involve many legal or ethical problems...
Differentiation of mesenchymal cells derived from human amniotic membranes into hepatocyte-like cells in vitroTomoharu Tamagawa
Institute of Cell Biology, Ishiwata Hospital, RIKEN BioResource Centre, Ibaraki, Japan
Hum Cell 20:77-84. 2007..Although further studies should be carried out to determine whether such in vitro-differentiated cells can function in vivo as hepatocytes. These cells may be useful in various applications that require human hepatocytes...
The potential of amniotic membrane/amnion-derived cells for regeneration of various tissuesAyaka Toda
Department of Regenerative Medicine, University of Toyama Graduate School of Medicine and Pharmaceutical Sciences, 2630 Sugitani, Toyama 930 0194 Japan
J Pharmacol Sci 105:215-28. 2007..It is of interest as a potential alternative to complicated tissue/organ transplantation. Recently, amnion-derived cells have been reported to have multipotent differentiation ability, and these cells have attracted ..
Xenografted human amniotic membrane-derived mesenchymal stem cells are immunologically tolerated and transdifferentiated into cardiomyocytesHiroko Tsuji
Department of Obstetrics, Keio University School of Medicine, Tokyo, Japan
Circ Res 106:1613-23. 2010..Amniotic membrane is known to have the ability to transdifferentiate into multiple organs and is expected to stimulate a reduced immunologic reaction...
Immunogenicity of human amniotic membrane in experimental xenotransplantationM Kubo
Department of Ophthalmology, Tokyo Medical University, Tokyo, Japan
Invest Ophthalmol Vis Sci 42:1539-46. 2001..The immunogenic characterization of amniotic membrane is still unknown. This study was designed to examine the immunogenicity of human amniotic membrane, by using experimental xenotransplantation models...
Efficient reprogramming of human and mouse primary extra-embryonic cells to pluripotent stem cellsShogo Nagata
Stem Cell Engineering, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara cho, Shogoin, Sakyo ku, Kyoto 606 8507, Japan
Genes Cells 14:1395-404. 2009..Here, we demonstrate that newborn human (h) and mouse (m) extra-embryonic amnion (AM) and yolk-sac (YS) cells, in which endogenous KLF4/Klf4, c-MYC/c-Myc and RONIN/Ronin are expressed, can be ..
Human amniotic epithelial cells possess hepatocyte-like characteristics and functionsSeiji Takashima
Department of Organ Regeneration, Institutes of Organ Transplants, Reconstructive Medicine and Tissue Engineering, Shinshu University Graduate School of Medicine, Asahi, Matsumoto, Japan
Cell Struct Funct 29:73-84. 2004..In organ culture, the amnion secreted 30-fold larger amounts of albumin than human amniotic epithelial cells in monolayer culture...
Transplanted human amniotic epithelial cells express connexin 26 and Na-K-adenosine triphosphatase in the inner earIsamu Yuge
Department of Otorhinolaryngology, Shinshu University School of Medicine, Asahi, Matsumoto, Japan
Transplantation 77:1452-4. 2004..This technology suggests the therapeutic potential for the treatment of hearing loss...
Rapid and efficient reprogramming of human amnion-derived cells into pluripotency by three factors OCT4/SOX2/NANOGHong xi Zhao
Department of Gynecology and Obstetrics, Tangdu Hospital, The Fourth Military Medical University, Xi an 710038, China
Differentiation 80:123-9. 2010..In this study, we tested whether human amnion-derived cells (hADCs) could be rapidly and efficiently reprogrammed into iPS cells by the defined factors: OCT4/..
IL-1beta and IL-8 in human fetal membranes: changes with gestational age, labor, and culture conditionsC L Elliott
Institute of Obstetrics and Gynaecology, Imperial College School of Medicine, London, UK
Am J Reprod Immunol 46:260-7. 2001..This study aimed to characterize their concentrations in fetal membranes and any changes in these with advancing gestation and to define as to whether there are interactions between the membranes in their expression...
Nuclear factor-kappa B is essential for up-regulation of interleukin-8 expression in human amnion and cervical epithelial cellsC L Elliott
Institute of Obstetrics and Gynaecology, Imperial College School of Medicine, Queen Charlotte s Hospital, Goldhawk Road, London W6 0XG, UK
Mol Hum Reprod 7:787-90. 2001..these sites to examine the relative importance of each site in IL-8 gene expression in a cervical cell line and in amnion cells obtained before and after labour...
Apoptosis in the normal human amnion at term, independent of Bcl-2 regulation and onset of labourK Kumagai
Department of Obstetrics and Gynecology, Osaka Medical College, 2-7 Daigaku-machi, Takatsuki, Osaka 569-8686, Japan
Mol Hum Reprod 7:681-9. 2001This study was designed to detect apoptosis in the human amnion and to elucidate the signalling pathway involved in its regulation...
Amniotic mesenchymal tissue cells inhibit dendritic cell differentiation of peripheral blood and amnion resident monocytesMarta Magatti
Centro di Ricerca E Menni, Fondazione Poliambulanza Istituto Ospedaliero, Brescia, Italy
Cell Transplant 18:899-914. 2009..We have previously shown that cells derived from the mesenchymal region of human amnion (AMTC) can strongly inhibit T-lymphocyte proliferation...
Amniotic membrane and amniotic fluid-derived cells: potential tools for regenerative medicine?Ornella Parolini
Centro di Ricerca E Menni, Fondazione Poliambulanza Istituto Ospedaliero, Via Bissolati, 57, 25124 Brescia, Italy
Regen Med 4:275-91. 2009....
Transplantation of human amniotic cells exerts neuroprotection in MPTP-induced Parkinson disease miceXiang Ying Kong
Department of Pharmacology, School of Basic Medicine, Peking Union Medical College and Institute of Basic Medical Science, Chinese Academy of Medical Sciences, Beijing, 100005 China
Brain Res 1205:108-15. 2008..The results suggested that transplanted HACs could survive and promote the endogenous neurogenesis of mice, which maybe related to the increased level of neurotrophins of the striatum...
Human amniotic epithelial cells ameliorate behavioral dysfunction and reduce infarct size in the rat middle cerebral artery occlusion modelTianjin Liu
Genetic Engineering Group, Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai, China
Shock 29:603-11. 2008..This model holds tremendous potential for development into wide use in cell-mediated gene therapy in the future...
Co-transplantation of bFGF-expressing amniotic epithelial cells and neural stem cells promotes functional recovery in spinal cord-injured ratsXiao ting Meng
Department of Histology and Embryology, School of Bethune Medical Science, Jilin University, Changchun 130021, PR China
Cell Biol Int 32:1546-58. 2008..These findings suggest that a cograft of AECs/pLEGFP-hbFGF and NSCs may have benefits for SCI...
Association of PAT proteins with lipid storage droplets in term fetal membranesW E Ackerman
Department of Obstetrics and Gynecology, Division of Maternal Fetal Medicine and Laboratory of Perinatal Research, The Ohio State University, College of Medicine, Columbus, OH 43210, USA
Placenta 28:465-76. 2007..We found that large LDs of amnion epithelial cells were reactive for neutral lipid stains and simultaneously encoated with ADRP and TIP47, but not ..
Characterization of immortalized human corneal endothelial cell line using HPV 16 E6/E7 on lyophilized human amniotic membraneHyun Ju Kim
Department of Ophthalmology, Chung Ang University Yongsan Hospital, Seoul, Korea
Korean J Ophthalmol 20:47-54. 2006..To establish the immortalized human corneal endothelial cell line (IHCEn) by transducing human papilloma virus (HPV) 16 E6/E7 oncogenes, and to identify their characteristics when cultivated on a lyophilized human amniotic membrane (LAM)...
Changes in culture expanded human amniotic epithelial cells: implications for potential therapeutic applicationsGita Pratama
Centre for Reproduction and Development, Monash Institute of Medical Research, Monash University, Clayton, Victoria, Australia
PLoS ONE 6:e26136. 2011..These findings suggest that primary and expanded hAEC may be suitable for different cellular therapeutic applications...
Phenotypic shift of human amniotic epithelial cells in culture is associated with reduced osteogenic differentiation in vitroG Stadler
Ludwig Boltzmann Institute for Experimental and Clinical Traumatology AUVA Research Center, Linz Vienna, Austria
Cytotherapy 10:743-52. 2008....
Production of hepatocyte-like cells from human amnionToshio Miki
Departments of Pathology and Surgery and McGowan Institute for Regenerative Medicine, University of Pittsburgh, USA
Methods Mol Biol 481:155-68. 2009..Cells from the placental tissues such as amnion and chorion have been reported to display multilineage differentiation and surface marker and gene expression ..
Role of glucocorticoid receptor and CCAAT/enhancer-binding protein alpha in the feed-forward induction of 11beta-hydroxysteroid dehydrogenase type 1 expression by cortisol in human amnion fibroblastsZhen Yang
School of Life Sciences, Fudan University, 220 Handan Road, Shanghai 200433, People s Republic of China
J Endocrinol 195:241-53. 2007..We examined the molecular mechanisms of the feedback effect of cortisol on 11beta-HSD1 mRNA expression in human amnion fibroblasts...
Protection of human amniotic epithelial cells (HAEC) from complement-mediated lysis: expression on the cells of three complement inhibitory membrane proteinsI A Rooney
Department of Medical Biochemistry, UWCM, Heath Park, Cardiff, U K
Immunology 71:308-11. 1990..Percentage cell lysis was markedly increased in the presence of Fab2 anti-CD59 and to a lesser, but significant, extent in the presence of Fab2 anti-DAF. Fab2 anti-MIP did not alter the sensitivity of the cells to lysis by complement...
Inducing proliferation of human amniotic epithelial (HAE) cells for cell therapyS Terada
Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Fukui University, Japan
Cell Transplant 9:701-4. 2000Probably because amnion is derived from the fetus and is exposed to the maternal immune system, human amniotic epithelial (HAE) cells do not express the HLA-A, -B, -C, or -DR antigens on their surfaces, suggesting that HAE cells do not ..
The human amnion is a site of MHC class Ib expression: evidence for the expression of HLA-E and HLA-GJ M Houlihan
University of Bristol, Department of Obstetrics and Gynecology, St Michael s Hospital, UK
J Immunol 154:5665-74. 1995The expression of HLA class I Ag by term human amnion epithelial cells was investigated...
Interstitial collagen synthesis and processing in human amnion: a property of the mesenchymal cellsM L Casey
Cecil H and Ida Green Center for Reproductive Biology Sciences, University of Texas, Southwestern Medical Center, Dallas 75235, USA
Biol Reprod 55:1253-60. 1996This study was conducted to evaluate the ontogeny and cellular site of interstitial collagen formation in amnion, the tissue that provides the tensile strength of the human fetal membranes...
Immunosuppressive properties of human amniotic membrane for mixed lymphocyte reactionM Ueta
Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan
Clin Exp Immunol 129:464-70. 2002..These results indicate that human AM has the ability to suppress allo-reactive T cells in vitro. This inhibitory effect likely contributes to the success of the ALT-AMT combination...
Evidence of in vitro differential secretion of 72 and 92 kDa type IV collagenases after selective exposure to lipopolysaccharide in human fetal membranesGuadalupe Garcia Lopez
Direction of Research, Instituto Nacional de Perinatologia Isidro Espinosa de los Reyes, Mexico City 11000, Mexico
Mol Hum Reprod 13:409-18. 2007..the chorioamniotic membranes form two independent chambers, allowing for the selective stimulation of either the amnion (AMN) and/or the choriodecidua (CHD) regions...
Biomechanical characterization of human amniotic membrane preparations for ocular surface reconstructionRoy S Chuck
Department of Ophthalmology, University of California, Irvine, Calif, USA
Ophthalmic Res 36:341-8. 2004..To investigate the tensile and elastic properties of both commercially available and experimental human amniotic membrane preparations...
Inducing proliferation of human amnion epithelial and mesenchymal cells for prospective engineering of membrane repairNicole Ochsenbein-Kölble
Unit of Perinatal Physiology, Department of Obstetrics, University Hospital, Zurich, Switzerland
J Perinat Med 31:287-94. 2003..approach to fetal membrane repair after premature rupture of the membranes (PROM) by characterizing the proliferation potential of human amnion epithelial and mesenchymal cells from preterm and term placenta in primary culture.
On the origin of amniotic stem cells: of mice and menMariya P Dobreva
Laboratory of Developmental Signaling, Department of Molecular and Developmental Genetics, VIB, KU Leuven, Belgium
Int J Dev Biol 54:761-77. 2010..The amnion is the innermost extraembryonic membrane that eventually surrounds the fetus of amniotes, and contains the ..
Toll-like receptor-2 and -4 in the chorioamniotic membranes in spontaneous labor at term and in preterm parturition that are associated with chorioamnionitisYeon Mee Kim
Perinatology Research Branch, National Institute of Child Health and Human Development/NIH/DHHS, Bethesda, MD, USA
Am J Obstet Gynecol 191:1346-55. 2004..These observations have implications for understanding the biologic nature of innate immunity...
Amnion-derived pluripotent/multipotent stem cellsToshio Miki
Department of Pathology, University of Pittsburgh, 200 Lothrop, St. Pittsburgh, PA 15213, USA
Stem Cell Rev 2:133-42. 2006..In this review, we describe the unique characteristics of the AE stem cells and summarize previous work concerning the stem cell nature of cells from amnion.
Immunogenicity and immunomodulatory effects of amnion-derived multipotent progenitor cellsRichard Allan Banas
Stemnion, Inc, Pittsburgh, PA, USA
Hum Immunol 69:321-8. 2008This is the first study on the immunologic properties of a clinically relevant population of cells derived from the amnion of human placenta...
Properties of the amniotic membrane for potential use in tissue engineeringHassan Niknejad
Department of Nanomedicine and Tissue Engineering, Shaheed Beheshti Medical University, Tehran, Iran
Eur Cell Mater 15:88-99. 2008..In this review, the various properties of the AM are discussed in light of their potential use for TE...
Fetal stem cells from extra-embryonic tissues: do not discardAkiva J Marcus
The Ira B Black Center for Stem Cell Research and the Department of Neuroscience and Cell Biology, University of Medicine and Dentistry of New Jersey Robert Wood Johnson Medical School, Piscataway, NJ 08854 5635, USA
J Cell Mol Med 12:730-42. 2008..In this review we summarize recent work examining the plasticity and clinical potential of fetal stem cells isolated from extra-embryonic tissues...
Suppression of transforming growth factor-beta isoforms, TGF-beta receptor type II, and myofibroblast differentiation in cultured human corneal and limbal fibroblasts by amniotic membrane matrixS C Tseng
Ocular Surface and Tear Center, Department of Ophthalmology, Bascom Palmer Eye Institute, University of Miami School of Medicine, Florida, USA
J Cell Physiol 179:325-35. 1999..It may also explain in part why fetal wound healing is scarless...
Amniotic membrane transplantation for ocular surface reconstructionS C Tseng
Ocular Surface and Tear Center, Bascom Palmer Eye Institute, William L McKnight Vision Research Pavillion, Miami, FL 33136, USA
Biosci Rep 21:481-9. 2001The use of amniotic membrane (or amnion) for transplantation as graft in ocular surface reconstruction is reviewed...
Basement membrane dissolution and reassembly by limbal corneal epithelial cells expanded on amniotic membraneWei Li
Ocular Surface Center and TissueTech, Inc, Miami, Florida 33173, USA
Invest Ophthalmol Vis Sci 47:2381-9. 2006..Thus, BM resynthesis may be used as another objective readout for assessing the success of ex vivo expansion of limbal epithelial progenitor cells on AM...
Region-specific gene expression profiling: novel evidence for biological heterogeneity of the human amnionYu Mi Han
Perinatology Research Branch, National Institute of Child Health and Human Development, National Institutes of Health, Department of Health and Human Services, Bethesda, Maryland 20892, USA
Biol Reprod 79:954-61. 2008The amnion plays an important role during pregnancy and parturition. Though referred to as a single structure, this fetal tissue is regionally divided into placental amnion, reflected amnion, and umbilical amnion...
Amnion-derived cellular cytokine solution (ACCS) promotes migration of keratinocytes and fibroblastsM Georgina Uberti
Institute for Tissue Regeneration, Repair, and Rehabilitation, Bay Pines VA Healthcare System, Bay Pines, FL, USA
Ann Plast Surg 64:632-5. 2010b>Amnion-derived Multipotent Progenitor cells appear to be useful as adjuvants in wound healing...
Human amnion mesenchyme harbors cells with allogeneic T-cell suppression and stimulation capabilitiesMarta Magatti
Centro di Ricerca E Menni, Fondazione Poliambulanza, Istituto Ospedaliero, Via Bissolati 57, I 25124 Brescia, Italy
Stem Cells 26:182-92. 2008..In this study, we isolated cells from the mesenchymal region of amnion and identified two subpopulations discordant for expression of the HLA-DR, CD45, CD14, and CD86 cellular markers...
Topical application of culture supernatant from human amniotic epithelial cells suppresses inflammatory reactions in corneaKazutaka Kamiya
Department of Ophthalmology, University of Tokyo School of Medicine, Tokyo, Japan
Exp Eye Res 80:671-9. 2005..These results suggest that HAEC are a source of soluble anti-inflammatory factors and that conditioned medium from HAEC contains factors other than IL-1ra that suppress corneal inflammation...
Successful vitrification of human amnion-derived mesenchymal stem cellsJeong Hee Moon
Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital, 300 Gumi, Bundang, Seongnam, Gyeonggi 463 707, Korea
Hum Reprod 23:1760-70. 2008A cryopreservation protocol for human amnion-derived mesenchymal stem cells (HAMs) is required because these cells cannot survive for long periods in culture...
Reconstruction of ocular surface with heterologous limbal epithelium and amniotic membrane in a rabbit modelM Avila
Departamento de Oftalmologia, Facultad de Medicina, Hospital San Juan de Dios, Universidad Nacional de Colombia, Colombia
Cornea 20:414-20. 2001..To report in vivo reconstruction of the ocular surface using amniotic membrane and heterologous transplants of epithelial limbal cells in rabbits with chemical burns...
Immunological characteristics of amniotic epitheliumJunko Hori
Department of Ophthalmology, Nippon Medical School, Sendagi 1 1 5, Bunkyo ku, Tokyo 113 8602, Japan
Cornea 25:S53-8. 2006..We therefore suggest that immunogenicity of AE should not be ignored, and use of AM from different donor placentas should be emphasized when repeated AM transplantation is required in patients clinically...
Prostaglandin H synthase-2 gene regulation in the amnion at labour: histone acetylation and nuclear factor kappa B binding to the promoter in vivoC M Mitchell
Mothers and Babies Research Centre, Hunter Medical Research Institute, Newcastle, Australia
Mol Hum Reprod 14:53-9. 2008Increased prostaglandin H synthase-2 (PGHS-2) expression in the amnion is critical for the production of prostaglandins that induce labour...
Smad5 determines murine amnion fate through the control of bone morphogenetic protein expression and signalling levelsErika A Bosman
Department of Developmental Biology VIB7 for Biotechnology VIB and Laboratory of Molecular Biology Celgen, University of Leuven, B 3000 Leuven, Belgium
Development 133:3399-409. 2006..It is essential for primordial germ cell (PGC) development, for the development of the allantois and for amnion closure, as demonstrated by loss of Bmp signalling...
Multilineage potential of side population cells from human amnion mesenchymal layerM Kobayashi
Department of Regenerative Medicine, School of Allied Health Sciences, Kitasato University, Nishi hashimoto, Kanagawa, Japan
Cell Transplant 17:291-301. 2008Side population (SP) cells were isolated by FACS from a human amnion mesenchymal cell (AMC) layer soon after enzyme treatment. The yield of SP cells from AMC layer (AMC-SP cells) was about 0.1-0.2%...
The effect of mechanical stretch on cyclooxygenase type 2 expression and activator protein-1 and nuclear factor-kappaB activity in human amnion cellsAarthi R Mohan
Imperial College London, Parturition Research Group, Institute of Reproductive and Developmental Biology, Hammersmith Hospital Campus, Du Cane Road, London W12 ONN, United Kingdom
Endocrinology 148:1850-7. 2007..Uterine stretch also leads to stretch of the fetal membranes, including the amnion, an important source of prostaglandin E2 (PGE2)...
Invasive pathway of Listeria ivanovii in human amnion-derived WISH cellsM G Ammendolia
Department of Technology and Health, National Institute of Health, Rome, Italy
Int J Immunopathol Pharmacol 20:509-18. 2007..monocytogenes. However, although L. ivanovii is capable of specifically infecting and replicating in human amnion cells, its survival in cytoplasm is limited compared to that of L. monocytogenes.
Amniotic membrane use in ophthalmologyJose A P Gomes
Advanced Ocular Surface Center, Brazil
Curr Opin Ophthalmol 16:233-40. 2005..The purpose of this review is to describe the most recent and relevant clinical and experimental data about the use of amniotic membrane in ophthalmology...
Evidence for a spatial and temporal regulation of prostaglandin-endoperoxide synthase 2 expression in human amnion in term and preterm parturitionDeug Chan Lee
Perinatology Research Branch, National Institute of Child Health and Human Development National Institutes of Health Department of Health and Human Services, Bethesda, Maryland, 20892, and Detroit, Michigan, 48201, USA
J Clin Endocrinol Metab 95:E86-91. 2010..PTGS2 mRNA was found to be differentially expressed between placental amnion (amnion overlying the placental disc) and reflected amnion (amnion of the extraplacental chorioamniotic membranes) ..
Amniotic membrane transplantation for conjunctival tumorZhicong Chen
Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China
Yan Ke Xue Bao 19:165-7, 145. 2003..CONCLUSION: Preserved AMT is a very effective method to repair wound after giant conjunctival tumor operation. Complete removal of tumor and perfect fixation are the key of ocular surface reconstruction...
A serum- and feeder-free technique of culturing human corneal epithelial stem cells on amniotic membraneKaevalin Lekhanont
Department of Ophthalmology, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand
Mol Vis 15:1294-302. 2009..To describe a simple technique of cultivating human corneal epithelial stem cells using an Epilife culture medium under serum- and feeder-free conditions...
Induced in vitro differentiation of pancreatic-like cells from human amnion-derived fibroblast-like cellsTomoharu Tamagawa
Institute of Cell Biology, Ishiwata Hospital, Mito, Ibaraki, Japan
Hum Cell 22:55-63. 2009There is growing evidence that the human amnion contains various types of stem cells. As amniotic tissue is readily available, it has the potential to be an important source of material for regenerative medicine...
In situ immunolabeling allows for detailed localization of prostaglandin synthesizing enzymes within amnion epitheliumW E Ackerman
Laboratory of Perinatal Research, Department of Obstetrics and Gynecology, Division of Maternal-Fetal Medicine, The Ohio State University, College of Medicine, Columbus, 43210, USA
Placenta 27:919-23. 2006Detailed information regarding the subcellular distribution of proteins within amnion epithelial cells is a goal of numerous placental biologists...
The persistence of transplanted amniotic membrane in corneal stromaChe J Connon
Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan
Am J Ophthalmol 141:190-2. 2006..To characterize the long-term incorporation of transplanted amniotic membrane into corneal stroma...
Decreased adherence and spontaneous separation of fetal membrane layers--amnion and choriodecidua--a possible part of the normal weakening processA Strohl
Departments of Pediatrics, Case Western Reserve University CWRU, MetroHealth Medical Center, 2500 MetroHealth Drive, Cleveland, OH, USA
Placenta 31:18-24. 2010The fetal membrane (FM) layers, amnion and choriodecidua, are frequently noted to have varying degrees of separation following delivery. FM layers normally separate prior to rupture during in vitro biomechanical testing...
Telomerase immortalized human amnion- and adipose-derived mesenchymal stem cells: maintenance of differentiation and immunomodulatory characteristicsSusanne Wolbank
Red Cross Blood Transfusion Service of Upper Austria, Linz, Austria
Tissue Eng Part A 15:1843-54. 2009..Sources such as adipose tissue and amnion offer outstanding possibilities for allogeneic transplantation due to their high differentiation potential and ..
Separation of amnion from choriodecidua is an integral event to the rupture of normal term fetal membranes and constitutes a significant component of the work requiredSunny Arikat
Department of Pediatrics, Case Western Reserve University, Cleveland, OH, USA
Am J Obstet Gynecol 194:211-7. 2006..FM) rupture and to compare the biophysical properties of intact FM with its separated individual components (amnion and choriodecidua)...
The use of amniotic membrane in reducing adhesions after strabismus surgeryHosam Sheha
Research Institute of Ophthalmology, Cairo, Egypt
J AAPOS 13:99-101. 2009..Different techniques have been used with varying success to reduce these adhesions. We describe a novel surgical technique in which amniotic membrane is used to wrap the extraocular muscles...
HOXC10 as a potential marker for discriminating between amnion- and decidua-derived mesenchymal stem cellsJong Ha Hwang
Department of Obstetrics and Gynecology, Korea University Guro Hospital, Korea University College of Medicine, Seoul, Korea
Cloning Stem Cells 11:269-79. 2009..HOXC10 was expressed in 10 of 10 amnion-derived MSCs but in only 2 of 10 decidua-derived MSCs...
Effects of amniotic membrane suspension in human corneal wound healing in vitroJin A Choi
Department of Ophthalmology and Visual Science, Kangnam St Mary s Hospital, College of Medicine, The Catholic University of Korea, Seoul, ROK
Mol Vis 15:2230-8. 2009..To investigate the biochemical mechanism of amniotic membrane (AM) suspension on corneal wound healing, particularly on epithelial proliferation and migration...
Characterization of the transcriptome of chorioamniotic membranes at the site of rupture in spontaneous labor at termChia Ling Nhan-Chang
Perinatology Research Branch, Intramural Division, Eunice Kennedy Shriver National Institute of Child Health and Human Development, NICHD NIH DHHS, Bethesda, MD, USA
Am J Obstet Gynecol 202:462.e1-41. 2010..The purpose of this study was to compare the transcriptome between the site of membrane rupture and the chorioamniotic membranes away from the site of rupture...
Amniochorion gelatinase-gelatinase inhibitor imbalance in vitro: a possible infectious pathway to ruptureS J Fortunato
Maternal Fetal Group and Perinatal Research Center of the Women s Health Research and Education Foundation and Aquinas College, Nashville, Tennessee 37203, USA
Obstet Gynecol 95:240-4. 2000..To estimate the effect of lipopolysaccharide on gelatinases and tissue inhibitors of matrix metalloproteinase 2 (gelatinase inhibitor) balance in human fetal membranes...
Multilayered amniotic membrane transplantation for severe ulceration of the cornea and scleraK Hanada
Department of Ophthalmology, Tokyo Dental College, Chiba, Japan
Am J Ophthalmol 131:324-31. 2001..To examine the efficacy of amniotic membrane transplantation in the treatment of deep corneal and scleral ulcers...
Amnionless, essential for mouse gastrulation, is mutated in recessive hereditary megaloblastic anemiaStephan M Tanner
Human Cancer Genetics Program, Comprehensive Cancer Center, The Ohio State University, Columbus, Ohio 43210, USA
Nat Genet 33:426-9. 2003..Mice homozygous with respect to the amn mutation generated by a transgene insertion have no amnion. The embryos are severely compromised, surviving to the tenth day of gestation but seem to lack the mesodermal ..
Paradoxical proinflammatory actions of interleukin-10 in human amnion: potential roles in term and preterm labourMurray D Mitchell
Liggins Institute, Department of Pharmacology, University of Auckland, Faculty of Medical and Health Sciences, Private Bag 92019, Auckland, New Zealand
J Clin Endocrinol Metab 89:4149-52. 2004..we have found that IL-10 does indeed exert anti-inflammatory properties in choriodecidua, but that in the adjacent amnion it has remarkable pro-inflammatory actions...
Chorioamniotic membranes constitute a competent barrier to group b streptococcus in vitroN Kjaergaard
Department of Obstetrics and Gynecology, Aalborg Hospital, Denmark
Eur J Obstet Gynecol Reprod Biol 83:165-9. 1999..To study the penetration of group B streptococcus (GBS) through human chorioamniotic membranes in vitro...
[Application of amnion membrane transplantation combine with mitomycin C in the treatment of pterygium]Yixia Huang
Shantou Center Hospital, Shantou 515031, China
Yan Ke Xue Bao 20:74-6. 2004..RESULT: Following up 6 to 18 months, the rate of recurrence is 3%. CONCLUSION: Applying amniotic membrane transplantation with mitomycin in operation can decrease pterygium recurrence, and no serious complication happens...
Secretions of interleukin-1beta and tumor necrosis factor alpha by whole fetal membranes depend on initial interactions of amnion or choriodecidua with lipopolysaccharides or group B streptococciVeronica Zaga
Biomedical Research Branch, Instituto Nacional de Perinatologia, Mexico City, Mexico
Biol Reprod 71:1296-302. 2004..the microbial insult using an in vitro experimental model that allowed testing of the individual contributions of amnion and choriodecidua to stimulation...
Comparison of characteristics of cultured limbal cells on denuded amniotic membrane and fresh conjunctival, limbal and corneal tissuesHossein Baharvand
Department of Stem Cells, Royan Institute, PO Box 19395 4644, Tehran, Iran
Dev Growth Differ 49:241-51. 2007..Also, the expression of traditional SC markers may not be a reliable indicator of limbal SC and there is an increasing need to determine factor(s) involved in their stemness...
Differential gene expression in the amnion, chorion, and trophoblast of the human placentaC A Plouzek
Human Genetics Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892
Placenta 14:277-85. 1993..Immunocytochemical studies suggest that PSG and hCG are also present in the human amnion. In this study, we examined whether amniotic and chorionic membranes were capable of expressing trophoblastic-..
Proteome analysis of human amnion and amniotic fluid by two-dimensional electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometrySoo-Jin Park
Department of Chemistry, Seoul National University, Gwanak-gu, Seoul, Korea 151-747
Proteomics 6:349-63. 2006Proteome analysis by 2-DE and PMF by MALDI-TOF MS was performed on human amnion and amniotic fluid at term. Ninety-two soluble and nineteen membrane proteins were identified from amnion...
Amniotic membrane transplantation with or without limbal allografts for corneal surface reconstruction in patients with limbal stem cell deficiencyS C Tseng
Ocular Surface and Tear Center, Department of Ophthalmology, Bascom Palmer Eye Institute, Miami, Fla 33136, USA
Arch Ophthalmol 116:431-41. 1998..To examine whether amniotic membrane transplantation (AMT), in preparing the perilimbal stroma, enhances the success of allograft limbal transplantation (ALT)...
Identification of matrix metalloproteinase-9 in amniotic fluid and amniochorion in spontaneous labor and after experimental intrauterine infection or interleukin-1 beta infusion in pregnant rhesus monkeysFelipe Vadillo-Ortega
Department of Research Direction and Electron Microscopy, , Mexico City, Mexico
Am J Obstet Gynecol 186:128-38. 2002..Immunocytochemistry localized matrix metalloproteinase-9 protein to amnion and chorion epithelial and mesenchymal cells and decidual stromal cells...
A single nucleotide polymorphism in the matrix metalloproteinase-1 (MMP-1) promoter influences amnion cell MMP-1 expression and risk for preterm premature rupture of the fetal membranesToshio Fujimoto
Center for Research on Reproduction and Women's Health, University of Pennsylvania Medical Center, Philadelphia, Pennsylvania 19104, USA
J Biol Chem 277:6296-302. 2002..We investigated whether this polymorphism is functionally significant for MMP-1 expression in amnion cells and whether it is associated with preterm premature rupture of the membranes (PPROM)...
Reduction in corneal haze and apoptosis by amniotic membrane matrix in excimer laser photoablation in rabbitsM X Wang
Vanderbilt Laser Sight Center and Department of Ophthalmology and Visual Sciences, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 8808, USA
J Cataract Refract Surg 27:310-9. 2001..To determine whether preserved human amniotic membrane can reduce corneal haze and keratocyte apoptosis induced by excimer laser photoablation in rabbit corneas...
Functional and anatomic results of amnion vaginoplasty in young women with Mayer-Rokitansky-Küster-Hauser syndromeChristina Fotopoulou
Department of Gynecology and Obstetrics, Charite, Campus Virchow Clinic, University Hospital, Berlin, Germany
Fertil Steril 94:317-23. 2010..To evaluate the surgical outcome and the long-term anatomic and functional results in young women with Mayer-Rokitansky-Küster-Hauser Syndrome (MRKH) undergoing neovaginal creation with amniotic membranes...
A combined proteome and ultrastructural localization analysis of 14-3-3 proteins in transformed human amnion (AMA) cells: definition of a framework to study isoform-specific differencesJose M A Moreira
Department of Proteomics in Cancer, Institute of Cancer Biology and Danish Centre for Translational Breast Cancer Research DCTB, Danish Cancer Society, DK 2100 Copenhagen, Denmark
Mol Cell Proteomics 7:1225-40. 2008..subcellular localization of the 14-3-3 beta, epsilon, sigma, tau, and zeta protein isoforms in transformed human amnion (AMA) cells...
Amnioinfusion in preterm PROM: effects on amnion and cord histologyA Locatelli
Department of Obstetrics and Gynecology, University of Milano Bicocca, Monza, Italy
J Perinatol 28:97-101. 2008To investigate the effects of transabdominal amnioinfusion (TA) on the histology of amnion (A) and umbilical cord (UC).
Development, validation and application of a two-site enzyme-linked immunosorbent assay for activin-ABL W Evans
School of Biological and Molecular Sciences, Oxford Brookes University, UK
J Endocrinol 153:221-30. 1997..Activin-A was also found in the culture media from explants of human amnion, chorion, maternal decidua and placenta. In marked contrast, activin-AB was undetectable (< 0...
Monochorionic diamniotic infants without twin-to-twin transfusion syndromeLeandro Cordero
Division of Neonatal-Perinatal Medicine, Department of Pediatrics and Obstetrics, College of Medicine and Public Health, The Ohio State University, Columbus, OH 43210-1228, USA
J Perinatol 25:753-8. 2005..Although their perinatal mortality is low, the reported occurrence and the short- and long-term impacts of fetal and neonatal morbidities warrants attention...
Research Grants
- Cellular Mechanisms of Amniotic Fluid Volume RegulationCecilia Y Cheung; Fiscal Year: 2010..These studies will be carried out in ovine amnion cells in vitro and chronically catheterized ovine fetuses in vivo...
- REGULATION OF VEGF EXPRESSION IN PLACENTA AND MEMBRANESCECILIA CHEUNG; Fiscal Year: 2004..Recently, we found expression of vascular endothelial growth factor (VEGF) in the amnion and chorion, and this expression is increased under conditions of enhanced intramembranous absorption...
- MECHANISMS OF FETAL MEMBRANE RUPTUREJerome F Strauss; Fiscal Year: 2010..increases SERPINH1 promoter activity mitigating the effect of the 656 T allele;4) collagen synthesis is reduced in amnion fibroblasts carrying the 656 T allele, but not the 656 T allele in the presence of the 12 bp deletion;5) the ..
- MECHANISMS OF FETAL MEMBRANE RUPTUREJEROME STRAUSS; Fiscal Year: 2009..SERPINH1 promoter activity mitigating the effect of the 656 T allele; 4) collagen synthesis is reduced in amnion fibroblasts carrying the 656 T allele, but not the 656 T allele in the presence of the 12 bp deletion; 5) the ..
- MECHANISMS OF FETAL MEMBRANE RUPTUREJEROME STRAUSS; Fiscal Year: 2009..SERPINH1 promoter activity mitigating the effect of the 656 T allele; 4) collagen synthesis is reduced in amnion fibroblasts carrying the 656 T allele, but not the 656 T allele in the presence of the 12 bp deletion; 5) the ..
- Mouse Model For Amniotic Fluid Volume RegulationCECILIA CHEUNG; Fiscal Year: 2002..This pathway is constituted by a network of microvessels on the surface of the placenta and, in sheep, within the amnion and chorion as well...
- CATHEPSIN B-LIKE CYSTEINE PROTEINASES AND TUMOR INVASIONBonnie Sloane; Fiscal Year: 1991..tumor cell degradation of the basement membrane or isolated components and/or 4) tumor cell invasion through the amnion basement membrane...
- MECHANISMS OF FETAL MEMBRANE RUPTUREJerome F Strauss; Fiscal Year: 2010..increases SERPINH1 promoter activity mitigating the effect of the 656 T allele;4) collagen synthesis is reduced in amnion fibroblasts carrying the 656 T allele, but not the 656 T allele in the presence of the 12 bp deletion;5) the ..
- The Molecular Pathogenesis of Health Disparities in Inf*Emmet Hirsch; Fiscal Year: 2005..In the second phase of the project, we analyze human tissues (myometrium, placenta, chorion, amnion, decidua, amniocytes and blood) collected from approximately 1360 patients presenting in term and preterm labor in ..
- Fetal Membrane AQP Expression/Amniotic Fluid RegulationMichael Ross; Fiscal Year: 2004..In situ hybridization reveals that AQP8 is expressed in epithelial cells of human amnion and chorion, as well as trophoblasts of placenta...
- REGULATION OF PHOSPHOLIPASE A2 IN HUMAN AMNIONLeslie Myatt; Fiscal Year: 2007The amnion is a major site of PGE2 production during labor and is a rich source of arachidonic acid, the precursor of PG production...
- REGULATION OF PHOSPHOLIPASE A2 IN HUMAN AMNIONLeslie Myatt; Fiscal Year: 2009The amnion is a major site of PGE2 production during labor and is a rich source of arachidonic acid, the precursor of PG production...
- OXIDATIVE DAMAGE AND PREMATURE RUPTURE OF THE MEMBRANESJames Woods; Fiscal Year: 2004..laboratory demonstrate that, in physiologic concentrations, the ROS, hypochlorous acid and superoxide, do damage amnion epithelium and collagen I, the strongest component of chorioamnion...
- In-ovo vaccination/avian influenza/bird/humanDE CHU TANG; Fiscal Year: 2006..Both vaccines will be injected into the amnion of chicken embryos...
- ENDOCRINE AND PARACRINE RELATIONSHIPS IN PARTURITIONMILES NOVY; Fiscal Year: 2002..Aim 2 will describe the presence of androgen and glucocorticoid receptors (ARs, GRs) in rhesus amnion, chorion, decidua, and myometrium at 80 and 100 percent of gestation using immunohistochemistry and ribonuclease ..
- HYPOXIA EFFECTS ON AMNIOTIC FLUID VOLUMEROBERT BRACE; Fiscal Year: 2007..pathway will be determined by studying the effects of VEGF on transport in isolated amniotic membrane and amnion cells in culture...
- MECHANISMS OF FETAL MEMBRANE RUPTUREJEROME STRAUSS; Fiscal Year: 2005..We will determine whether proteolytic fragments of fibronectin induce MMP expression in human amnion cells in vitro; define the receptor(s) and signal transduction cascade underlying this response; identify active ..
