amnion

Summary

Summary: The innermost membranous sac that surrounds and protects the developing EMBRYO which is bathed in the AMNIOTIC FLUID. Amnion cells are secretory EPITHELIAL CELLS and contribute to the amniotic fluid.

Top Publications

  1. ncbi Concise review: isolation and characterization of cells from human term placenta: outcome of the first international Workshop on Placenta Derived Stem Cells
    Ornella Parolini
    Centro di Ricerca E Menni, Fondazione Poliambulanza, Istituto Ospedaliero, Via Bissolati 57, 25124 Brescia, Italy
    Stem Cells 26:300-11. 2008
  2. ncbi Stem cell characteristics of amniotic epithelial cells
    Toshio Miki
    Department of Pathology and McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA
    Stem Cells 23:1549-59. 2005
  3. ncbi Engraftment potential of human amnion and chorion cells derived from term placenta
    Marco Bailo
    Centro Ricerche Parco Scientifico E Menni, Ospedale Poliambulanza, Via Romiglia, 4, I 25124 Brescia, Italy
    Transplantation 78:1439-48. 2004
  4. doi Human amnion epithelial cells prevent bleomycin-induced lung injury and preserve lung function
    Sean Murphy
    The Ritchie Centre, Monash Institute of Medical Research, Monash University, Clayton, Australia
    Cell Transplant 20:909-23. 2011
  5. doi Human amnion epithelial cell transplantation abrogates lung fibrosis and augments repair
    Yuben Moodley
    F R A C P School of Medicine and Pharmacology, University of Western Australia, 50 Murray Street, Perth, Western Australia 6000, Australia
    Am J Respir Crit Care Med 182:643-51. 2010
  6. ncbi The role of inflammation and infection in preterm birth
    Roberto Romero
    Perinatology Research Branch, National Institute of Child Health and Human Development, National Institutes of Health, Department of Health and Human Services, Bethesda, Maryland, USA
    Semin Reprod Med 25:21-39. 2007
  7. doi Transplantation of allogeneic and xenogeneic placenta-derived cells reduces bleomycin-induced lung fibrosis
    Anna Cargnoni
    Centro di Ricerca E Menni, Fondazione Poliambulanza Istituto Ospedaliero, 25124 Brescia, Italy
    Cell Transplant 18:405-22. 2009
  8. ncbi Stem cells derived from human fetal membranes display multilineage differentiation potential
    Sivakami Ilancheran
    Center for Women s Health Research, Department of Obstetrics and Gynecology, Monash University, Monash Medical Center, Clayton, Victoria 3168, Australia
    Biol Reprod 77:577-88. 2007
  9. ncbi Amnion: a potent graft source for cell therapy in stroke
    Seong Jin Yu
    Department of Neurology, Medical College of Georgia, Augusta, GA, USA
    Cell Transplant 18:111-8. 2009
  10. doi Amniotic membrane and amniotic cells: potential therapeutic tools to combat tissue inflammation and fibrosis?
    U Manuelpillai
    Monash Institute of Medical Research, Monash University, Clayton, Victoria 3168, Australia
    Placenta 32:S320-5. 2011

Detail Information

Publications291 found, 100 shown here

  1. ncbi Concise review: isolation and characterization of cells from human term placenta: outcome of the first international Workshop on Placenta Derived Stem Cells
    Ornella Parolini
    Centro di Ricerca E Menni, Fondazione Poliambulanza, Istituto Ospedaliero, Via Bissolati 57, 25124 Brescia, Italy
    Stem Cells 26:300-11. 2008
    ....
  2. ncbi Stem cell characteristics of amniotic epithelial cells
    Toshio Miki
    Department of Pathology and McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA
    Stem Cells 23:1549-59. 2005
    ..b>Amnion derived from term placenta after live birth may be a useful and noncontroversial source of stem cells for cell ..
  3. ncbi Engraftment potential of human amnion and chorion cells derived from term placenta
    Marco Bailo
    Centro Ricerche Parco Scientifico E Menni, Ospedale Poliambulanza, Via Romiglia, 4, I 25124 Brescia, Italy
    Transplantation 78:1439-48. 2004
    ..which may entail progenitor potential. The immunologic reactivity and the transplantation potential of amnion and chorion cells, however, remain to be elucidated.
  4. doi Human amnion epithelial cells prevent bleomycin-induced lung injury and preserve lung function
    Sean Murphy
    The Ritchie Centre, Monash Institute of Medical Research, Monash University, Clayton, Australia
    Cell Transplant 20:909-23. 2011
    Human amnion epithelial cells (hAECs) have attracted recent attention as a promising source of cells for regenerative therapies, with reports that cells derived from human term amnion possess multipotent differentiation ability, low ..
  5. doi Human amnion epithelial cell transplantation abrogates lung fibrosis and augments repair
    Yuben Moodley
    F R A C P School of Medicine and Pharmacology, University of Western Australia, 50 Murray Street, Perth, Western Australia 6000, Australia
    Am J Respir Crit Care Med 182:643-51. 2010
    ..Cellular therapies that could restore pneumocytes and reduce inflammation and fibrosis would be a major advance in management...
  6. ncbi The role of inflammation and infection in preterm birth
    Roberto Romero
    Perinatology Research Branch, National Institute of Child Health and Human Development, National Institutes of Health, Department of Health and Human Services, Bethesda, Maryland, USA
    Semin Reprod Med 25:21-39. 2007
    ..This article reviews the role of inflammation in preterm and term parturition...
  7. doi Transplantation of allogeneic and xenogeneic placenta-derived cells reduces bleomycin-induced lung fibrosis
    Anna Cargnoni
    Centro di Ricerca E Menni, Fondazione Poliambulanza Istituto Ospedaliero, 25124 Brescia, Italy
    Cell Transplant 18:405-22. 2009
    Fetal membranes (amnion and chorion) have recently raised significant attention as potential sources of stem cells...
  8. ncbi Stem cells derived from human fetal membranes display multilineage differentiation potential
    Sivakami Ilancheran
    Center for Women s Health Research, Department of Obstetrics and Gynecology, Monash University, Monash Medical Center, Clayton, Victoria 3168, Australia
    Biol Reprod 77:577-88. 2007
    The amnion is the inner of two membranes surrounding the fetus. That it arises from embryonic epiblast cells prior to gastrulation suggests that it may retain a reservoir of stem cells throughout pregnancy...
  9. ncbi Amnion: a potent graft source for cell therapy in stroke
    Seong Jin Yu
    Department of Neurology, Medical College of Georgia, Augusta, GA, USA
    Cell Transplant 18:111-8. 2009
    ..Recently, amnion-derived cells have been reported to have multipotent differentiation ability, and these cells have attracted ..
  10. doi Amniotic membrane and amniotic cells: potential therapeutic tools to combat tissue inflammation and fibrosis?
    U Manuelpillai
    Monash Institute of Medical Research, Monash University, Clayton, Victoria 3168, Australia
    Placenta 32:S320-5. 2011
    ....
  11. ncbi Dose-dependent immunomodulatory effect of human stem cells from amniotic membrane: a comparison with human mesenchymal stem cells from adipose tissue
    Susanne Wolbank
    Red Cross Blood Transfusion Service of Upper Austria, Linz, Austria
    Tissue Eng 13:1173-83. 2007
    ..All three cell types may be considered as possible alternatives to BMSCs for allogeneic application in tissue engineering...
  12. ncbi The potential of amniotic membrane/amnion-derived cells for regeneration of various tissues
    Ayaka Toda
    Department of Regenerative Medicine, University of Toyama Graduate School of Medicine and Pharmaceutical Sciences, 2630 Sugitani, Toyama 930 0194 Japan
    J Pharmacol Sci 105:215-28. 2007
    ..It is of interest as a potential alternative to complicated tissue/organ transplantation. Recently, amnion-derived cells have been reported to have multipotent differentiation ability, and these cells have attracted ..
  13. doi Amniotic membrane and amniotic fluid-derived cells: potential tools for regenerative medicine?
    Ornella Parolini
    Centro di Ricerca E Menni, Fondazione Poliambulanza Istituto Ospedaliero, Via Bissolati, 57, 25124 Brescia, Italy
    Regen Med 4:275-91. 2009
    ....
  14. pmc Human amniotic epithelial cells as novel feeder layers for promoting ex vivo expansion of limbal epithelial progenitor cells
    Ying Ting Chen
    TissueTech Inc, and Ocular Surface Center, Miami, FL 33173, USA
    Stem Cells 25:1995-2005. 2007
    ..Disclosure of potential conflicts of interest is found at the end of this article...
  15. ncbi Immunosuppressive factors secreted by human amniotic epithelial cells
    Haochuan Li
    Department of Ophthalmology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    Invest Ophthalmol Vis Sci 46:900-7. 2005
    ....
  16. ncbi Isolation, characterization, and differentiation of stem cells derived from the rat amniotic membrane
    Akiva J Marcus
    The Ira B Black Center for Stem Cell Research, Department of Neuroscience and Cell Biology, University of Medicine and Dentistry of New Jersey Robert Wood Johnson Medical School, Piscataway, NJ 08854 5635, USA
    Differentiation 76:130-44. 2008
    ..Similar to mesenchymal stem cells, these amnion-derived stem cells (ADSCs) express the surface markers CD29 and CD90, but were negative for the ..
  17. ncbi Comparative characterization of cultured human term amnion epithelial and mesenchymal stromal cells for application in cell therapy
    Grozdana Bilic
    Department of Obstetrics, University Hospital Zurich, Zurich, Switzerland
    Cell Transplant 17:955-68. 2008
    Emerging evidence suggests human amnion tissue as a valuable source of two distinct types of pluripotent cells, amnion epithelial cells (hAECs) and mesenchymal stromal cells (hAMSCs), for applications in cell replacement therapy...
  18. doi Isolation and characterization of Oct-4+/HLA-G+ mesenchymal stem cells from human umbilical cord matrix: differentiation potential and detection of new markers
    Giampiero La Rocca
    Sezione di Anatomia Umana, Dipartimento di Medicina Sperimentale, Universita degli Studi di Palermo, Via del Vespro 129, 90127, Palermo, Italy
    Histochem Cell Biol 131:267-82. 2009
    ....
  19. ncbi Role of human amniotic epithelial cell transplantation in spinal cord injury repair research
    V Sankar
    Department of Anatomy, Dr Arcot Lakshmanaswamy Mudaliar Postgraduate Institute of Basic Medical Sciences, Taramani Campus, University of Madras, 600 113, Chennai, India
    Neuroscience 118:11-7. 2003
    ..First, there was no evidence of immunological rejection probably due to the non-antigenic nature of the HAEC. Second, unlike neural tissue, procurement of HAEC does not involve many legal or ethical problems...
  20. ncbi Antiinflammatory effects of amniotic membrane transplantation in ocular surface disorders
    S Shimmura
    Department of Ophthalmology, Tokyo Dental College, Tokyo, Japan
    Cornea 20:408-13. 2001
    ..CONCLUSION: Amniotic membrane attracts and traps inflammatory cells infiltrating the ocular surface, which may explain some of the antiinflammatory properties of the fetal tissue...
  21. pmc Surfactant protein-A as an anti-inflammatory component in the amnion: implications for human pregnancy
    Deug Chan Lee
    Perinatology Research Branch, Department of Health and Human Services, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, USA
    J Immunol 184:6479-91. 2010
    ..a potential role for SP-A in human pregnancy and parturition by examining SP-A expression patterns in AF and amnion. High molecular mass (>250 kDa) oligomeric SP-A was increased in AF with advancing gestation...
  22. pmc Changes in culture expanded human amniotic epithelial cells: implications for potential therapeutic applications
    Gita Pratama
    Centre for Reproduction and Development, Monash Institute of Medical Research, Monash University, Clayton, Victoria, Australia
    PLoS ONE 6:e26136. 2011
    ..These findings suggest that primary and expanded hAEC may be suitable for different cellular therapeutic applications...
  23. ncbi The amniotic membrane in ophthalmology
    Harminder S Dua
    Division of Ophthalmology and Visual Sciences, University of Nottingham, Nottingham, UK
    Surv Ophthalmol 49:51-77. 2004
    ..Further studies will undoubtedly reveal the true potential of the membrane, its mechanism(s) of action, and the effective use of this tissue in ophthalmology...
  24. doi Amniotic membrane application reduces liver fibrosis in a bile duct ligation rat model
    Luciana B Sant'anna
    Centro di Ricerca E Menni, Fondazione Poliambulanza Istituto Ospedaliero, Via Bissolati 57, Brescia, Italy
    Cell Transplant 20:441-53. 2011
    ..These findings suggest that human AM, when applied as a patch onto the liver surface, might inhibit fibrosis progression in BDL-injured livers, and could protect against hepatic damage associated with fibrotic degeneration...
  25. doi In vitro secretion profile of pro-inflammatory cytokines IL-1β, TNF-α, IL-6, and of human beta-defensins (HBD)-1, HBD-2, and HBD-3 from human chorioamniotic membranes after selective stimulation with Gardnerella vaginalis
    Veronica Zaga-Clavellina
    Cell Biology Department, Instituto Nacional de Perinatologia Isidro Espinosa de los Reyes, Mexico City, Mexico
    Am J Reprod Immunol 67:34-43. 2012
    ..Preterm labor associated with infection is a major clinical condition; in this work, we analyze the response of human chorioamniotic membranes stimulated with Gardnerella vaginalis...
  26. doi Labor and inflammation increase the expression of oxytocin receptor in human amnion
    Vasso Terzidou
    Imperial College London, Parturition Research Group, Institute of Reproductive and Developmental Biology, Hammersmith Hospital Campus, Du Cane Road, London, United Kingdom
    Biol Reprod 84:546-52. 2011
    The oxytocin/oxytocin receptor (OXT/OXTR) system plays an important role in the regulation of parturition. The amnion is a major source of prostaglandins and inflammatory cytokine synthesis, which increase both before and during labor...
  27. doi Clinical outcomes of xeno-free autologous cultivated limbal epithelial transplantation: a 10-year study
    Virender S Sangwan
    Cornea and Anterior Segment Services, L V Prasad Eye Institute, Kallam Anji Reddy Campus, Road No 2, Banjara Hills, Hyderabad 500034, India
    Br J Ophthalmol 95:1525-9. 2011
    ..Ocular burns can damage the corneal epithelial stem cells located at the limbus. This study evaluated the efficacy of xeno-free autologous cell-based treatment of limbal stem cell deficiency...
  28. ncbi Identification of stem cell marker-positive cells by immunofluorescence in term human amnion
    Toshio Miki
    Department of Pathology, University of Pittsburgh, Pittsburgh, PA 15213, USA
    J Reprod Immunol 75:91-6. 2007
    ..Stem cell marker-positive cells were found in amnion but not in choriodecidua, tissues known to contain hematopoietic and trophoblastic stem cells...
  29. ncbi Factors secreted by human amniotic epithelial cells promote the survival of rat retinal ganglion cells
    Saiko Uchida
    Department of Ophthalmology, Tokyo University School of Medicine, Tokyo 113 8655, Japan
    Neurosci Lett 341:1-4. 2003
    ..These results suggest that HAECs may produce unknown neuroprotective factors, suggesting its potential for the treatment of RGC degenerative diseases...
  30. pmc Epidermal growth factor and interleukin-1beta utilize divergent signaling pathways to synergistically upregulate cyclooxygenase-2 gene expression in human amnion-derived WISH cells
    William E Ackerman
    Department of Obstetrics and Gynecology Division of Maternal Fetal Medicine and Laboratory of Perinatal Research, The Ohio State University, College of Medicine and Public Health, Columbus, Ohio 43210, USA
    Biol Reprod 71:2079-86. 2004
    ..It was previously demonstrated that EGF could potentiate IL-1beta-driven PGE(2) production in amnion and amnion-derived (WISH) cells...
  31. ncbi Xenografted human amniotic membrane-derived mesenchymal stem cells are immunologically tolerated and transdifferentiated into cardiomyocytes
    Hiroko Tsuji
    Department of Obstetrics, Keio University School of Medicine, Tokyo, Japan
    Circ Res 106:1613-23. 2010
    ..Amniotic membrane is known to have the ability to transdifferentiate into multiple organs and is expected to stimulate a reduced immunologic reaction...
  32. ncbi Differentiation of mesenchymal cells derived from human amniotic membranes into hepatocyte-like cells in vitro
    Tomoharu Tamagawa
    Institute of Cell Biology, Ishiwata Hospital, RIKEN BioResource Centre, Ibaraki, Japan
    Hum Cell 20:77-84. 2007
    ..Although further studies should be carried out to determine whether such in vitro-differentiated cells can function in vivo as hepatocytes. These cells may be useful in various applications that require human hepatocytes...
  33. ncbi Suppression of transforming growth factor-beta isoforms, TGF-beta receptor type II, and myofibroblast differentiation in cultured human corneal and limbal fibroblasts by amniotic membrane matrix
    S C Tseng
    Ocular Surface and Tear Center, Department of Ophthalmology, Bascom Palmer Eye Institute, University of Miami School of Medicine, Florida, USA
    J Cell Physiol 179:325-35. 1999
    ..It may also explain in part why fetal wound healing is scarless...
  34. doi Human amniotic epithelial cells ameliorate behavioral dysfunction and reduce infarct size in the rat middle cerebral artery occlusion model
    Tianjin Liu
    Genetic Engineering Group, Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai, China
    Shock 29:603-11. 2008
    ..This model holds tremendous potential for development into wide use in cell-mediated gene therapy in the future...
  35. ncbi Human amniotic mesenchymal cells have some characteristics of cardiomyocytes
    Peng Zhao
    Department of Organ Regeneration, Institute of Organ Transplants, Reconstructive Medicine and Tissue Engineering, Shinshu University School of Medicine, Matsumoto, Japan
    Transplantation 79:528-35. 2005
    ..Here, we report that human amniotic mesenchymal cells (hAMC), which are multipotent cells derived from fetal mesoderm, may be a suitable cell source for CCM...
  36. doi Transplantation of human amnion epithelial cells reduces hepatic fibrosis in immunocompetent CCl₄-treated mice
    Ursula Manuelpillai
    Centre for Reproduction and Development, Monash Institute of Medical Research, Monash University, Clayton, Victoria, Australia
    Cell Transplant 19:1157-68. 2010
    ..To test this hypothesis we transplanted human amnion epithelial cells (hAEC), isolated from term delivered placenta, into immunocompetent C57/BL6 mice at week 2 of a 4-..
  37. ncbi Immunogenicity of human amniotic membrane in experimental xenotransplantation
    M Kubo
    Department of Ophthalmology, Tokyo Medical University, Tokyo, Japan
    Invest Ophthalmol Vis Sci 42:1539-46. 2001
    ..The immunogenic characterization of amniotic membrane is still unknown. This study was designed to examine the immunogenicity of human amniotic membrane, by using experimental xenotransplantation models...
  38. ncbi Efficient reprogramming of human and mouse primary extra-embryonic cells to pluripotent stem cells
    Shogo Nagata
    Stem Cell Engineering, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara cho, Shogoin, Sakyo ku, Kyoto 606 8507, Japan
    Genes Cells 14:1395-404. 2009
    ..Here, we demonstrate that newborn human (h) and mouse (m) extra-embryonic amnion (AM) and yolk-sac (YS) cells, in which endogenous KLF4/Klf4, c-MYC/c-Myc and RONIN/Ronin are expressed, can be ..
  39. ncbi Amniotic membrane use in ophthalmology
    Jose A P Gomes
    Advanced Ocular Surface Center, Brazil
    Curr Opin Ophthalmol 16:233-40. 2005
    ..The purpose of this review is to describe the most recent and relevant clinical and experimental data about the use of amniotic membrane in ophthalmology...
  40. ncbi Regulation of phospholipase isozymes by nuclear factor-kappaB in human gestational tissues in vitro
    Martha Lappas
    Department of Obstetrics and Gynaecology, The University of Melbourne and Mercy Perinatal Research Centre, Mercy Hospital for Women, East Melbourne, Victoria, Australia 3002
    J Clin Endocrinol Metab 89:2365-72. 2004
    ..Human placenta, amnion, and choriodecidua (n = 4-9 separate placentas) were incubated in the presence of SASP (0...
  41. pmc The effects of thrombin and cytokines upon the biomechanics and remodeling of isolated amnion membrane, in vitro
    D Kumar
    Department of Pediatrics, Case Western Reserve University, Cleveland, OH, USA
    Placenta 32:206-13. 2011
    ..The purpose of these studies was to determine whether thrombin and cytokines directly weaken the amnion membrane (AM), the major load-bearing component of FM...
  42. doi Propolis and amnion reepithelialise second-degree burns in rats
    Alicia Greyce Turatti Pessolato
    Faculty of Veterinary Medicine and Animal Science, Department of Surgery, University of Sao Paulo, São Paulo 05508 270, Sao Paulo, Brazil
    Burns 37:1192-201. 2011
    ..repair or healing of burns in rats that were treated either daily with 5% propolis ointment or by autologous amnion graft...
  43. ncbi Proteoglycans and hyaluronan in human fetal membranes
    M Meinert
    Research Laboratory, Department of Obstetrics and Gynecology, Aarhus University Hospital, Denmark
    Am J Obstet Gynecol 184:679-85. 2001
    ..RESULTS: The fetal membranes contained predominantly smaller proteoglycans, such as biglycan and decorin. The amnion consisted of typical fibrous connective tissue with a high concentration of collagen...
  44. ncbi Interleukin-8 release from human gestational tissue explants: the effects of lipopolysaccharide and cytokines
    N Laham
    Perinatal Research Centre, Department of Perinatal Medicine, The Royal Women s Hospital, Carlton, Victoria, Australia
    Biol Reprod 57:616-20. 1997
    ..In this study, we tested the hypotheses that IL-8 is released locally in the intrauterine environment from human amnion, choriodecidua, and placenta, and that IL-8 release from these tissues is increased by bacterial endotoxin, ..
  45. ncbi Properties of the amniotic membrane for potential use in tissue engineering
    Hassan Niknejad
    Department of Nanomedicine and Tissue Engineering, Shaheed Beheshti Medical University, Tehran, Iran
    Eur Cell Mater 15:88-99. 2008
    ..In this review, the various properties of the AM are discussed in light of their potential use for TE...
  46. ncbi Human amnion mesenchyme cells express phenotypes of neuroglial progenitor cells
    Norio Sakuragawa
    Department of Regenerative Medicine, Toho University School of Medicine, Sagamihara, Kanagawa, Japan
    J Neurosci Res 78:208-14. 2004
    Previous studies from our laboratory showed that human amnion epithelial cells (AECs) have multiple functions, such as synthesis and release of catecholamines, acetylcholine, neurotrophic factors, activin, and noggin...
  47. ncbi Protective effect of Astragali radix extract on interleukin 1beta-induced in fl ammation in human amnion
    Yun Hee Shon
    Department of Pharmacology, College of Medicine and Intractable Disease Research Center, Dongguk University, Kyongju 780 714, Korea
    Phytother Res 17:1016-20. 2003
    ..production and prostaglandin E(2) (PGE(2)) and leukotriene C(4) (LTC(4)) release from IL-1beta-stimulated human amnion. Primary monolayer cultures of amnion cells were established from women undergoing elective caesarean section ..
  48. pmc The involvement of human amnion in histologic chorioamnionitis is an indicator that a fetal and an intra-amniotic inflammatory response is more likely and severe: clinical implications
    Chan Wook Park
    Department of Obstetrics and Gynecology, Seoul National University College of Medicine, 28 Yongon Dong, Chongno Gu, Seoul 110 744, Republic of Korea
    Placenta 30:56-61. 2009
    Amnionitis (inflammation of the amnion) is the final stage of extra-placental chorioamniotic inflammation...
  49. pmc Nuclear factor kappa B activation occurs in the amnion prior to labour onset and modulates the expression of numerous labour associated genes
    Sheri Lim
    Imperial College Parturition Research Group, Institute of Reproduction and Developmental Biology, Imperial College London, London, United Kingdom
    PLoS ONE 7:e34707. 2012
    ..is an increase in the synthesis of prostaglandins, cytokines and chemokines in the fetal membranes, particular the amnion. This is associated with activation of the transcription factor nuclear factor kappa B (NFκB)...
  50. pmc The role of the multiple banded antigen of Ureaplasma parvum in intra-amniotic infection: major virulence factor or decoy?
    Samantha J Dando
    Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Queensland, Australia
    PLoS ONE 7:e29856. 2012
    ..This host response may be important in the pathogenesis of inflammation-mediated adverse pregnancy outcomes...
  51. ncbi Micronucleus formation in human amnion cells after exposure to 50 Hz MF applied horizontally and vertically
    M Simko
    Institute of Animal Physiology, Division of Environmental Physiology, University of Rostock, Universitatsplatz 2, D 18055, Rostock, Germany
    Mutat Res 418:101-11. 1998
    ..Therefore, the reason for inconsistent results published in the literature may be caused by the variability of exposure systems, the exposure conditions and the cell types used...
  52. ncbi Tocilizumab inhibits interleukin-6-mediated matrix metalloproteinase-2 and -9 secretions from human amnion cells in preterm premature rupture of membranes
    Yukio Mano
    Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine, Showa Ku, Nagoya, Japan
    Gynecol Obstet Invest 68:145-53. 2009
    ....
  53. pmc A serum- and feeder-free technique of culturing human corneal epithelial stem cells on amniotic membrane
    Kaevalin Lekhanont
    Department of Ophthalmology, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand
    Mol Vis 15:1294-302. 2009
    ..To describe a simple technique of cultivating human corneal epithelial stem cells using an Epilife culture medium under serum- and feeder-free conditions...
  54. ncbi Ocular surface changes in limbal stem cell deficiency caused by chemical injury: a histologic study of excised pannus from recipients of cultured corneal epithelium
    A Fatima
    Stem Cell Biology Laboratory, LV Prasad Eye Institute, Hyderabad, India
    Eye (Lond) 22:1161-7. 2008
    ..To report histopathologic changes of the ocular surface pannus in patients with severe limbal stem cell deficiency (LSCD)...
  55. pmc Amniotic mesenchymal stem cells: a new source for hepatocyte-like cells and induction of CFTR expression by coculture with cystic fibrosis airway epithelial cells
    Valentina Paracchini
    Medical Genetics Laboratory, Fondazione IRCCS Ca Granda Ospedale Maggiore Policlinico, Via Commenda 12, 20122 Milan, Italy
    J Biomed Biotechnol 2012:575471. 2012
    ..Our data show that amniotic MSCs have the potential to differentiate into epithelial cells of organs relevant in CF pathogenesis and may contribute to partial correction of the CF phenotype...
  56. ncbi Experimental study on allografts of amniotic epithelial cells in calcaneal tendon lesions of sheep
    A Muttini
    Department of Veterinary Clinical Sciences, University of Teramo, Teramo, Italy
    Vet Res Commun 34:S117-20. 2010
    ..In addition, after 1 month, the neo-collagen began to be organized into parallel arrays of fibers oriented along the longitudinal axis of the tendon...
  57. ncbi Amniotic membrane transplantation with conjunctival autograft for recurrent pterygium
    Jun Shimazaki
    Department of Ophthalmology, Tokyo Dental College, Chiba, Japan
    Ophthalmology 110:119-24. 2003
    ..To study the effect of amniotic membrane transplantation (AMT) combined with either limbal autograft transplantation (LAT) or conjunctival autograft transplantation (CAT) in recurrent pterygium...
  58. ncbi Isolation and characterization of mesenchymal stem cells from the amniotic membrane
    Carmen Mihaela Mihu
    Department of Histology, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj Napoca, Romania
    Rom J Morphol Embryol 50:73-7. 2009
    ..HLA expression provides information that might help explain the immunological mechanisms of tolerance between the maternal organism and fetal structures...
  59. doi Isolation, proliferation, cytogenetic, and molecular characterization and in vitro differentiation potency of canine stem cells from foetal adnexa: a comparative study of amniotic fluid, amnion, and umbilical cord matrix
    M Filioli Uranio
    Department of Animal Production, University of Bari Aldo Moro, Bari, Italy
    Mol Reprod Dev 78:361-73. 2011
    ..05). Amnion and UCM-MSCs expressed embryonic and MSC markers, such as Oct-4 CD44, CD184, and CD29, whereas AF-MSCs expressed ..
  60. ncbi Amnion-derived pluripotent/multipotent stem cells
    Toshio Miki
    Department of Pathology, University of Pittsburgh, 200 Lothrop, St Pittsburgh, PA 15213, USA
    Stem Cell Rev 2:133-42. 2006
    ..In this review, we describe the unique characteristics of the AE stem cells and summarize previous work concerning the stem cell nature of cells from amnion.
  61. ncbi Autophagy and endocytosis in the amnion
    Zhong Ying Shen
    Department of Pathology, Key Immunopathology Laboratory of Guangdong Province, Medical College of Shantou University, Shantou 515031, China
    J Struct Biol 162:197-204. 2008
    To search for the origin of nutrition in the amnion, we focused attention on both endocytotic and autophagic pathways. Using ultrastructural and biochemical methods, we examined 20 human amnions at term gestation...
  62. pmc Contribution of aquaporin 9 and multidrug resistance-associated protein 2 to differential sensitivity to arsenite between primary cultured chorion and amnion cells prepared from human fetal membranes
    Yuta Yoshino
    Department of Clinical Molecular Genetics, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, Horinouchi, Hachioji, Tokyo, Japan
    Toxicol Appl Pharmacol 257:198-208. 2011
    ..We examined the effects of As(III) on primary cultured chorion (C) and amnion (A) cells prepared from human fetal membranes...
  63. pmc Hepatic differentiation of amniotic epithelial cells
    Fabio Marongiu
    Department of Pathology, University of Pittsburgh, Pittsburgh, PA, USA
    Hepatology 53:1719-29. 2011
    ..When transplanted in a syngenic animal pretreated with RS, rAECs were able to engraft and generate a progeny of cells with morphology and protein expression typical of mature hepatocytes...
  64. pmc Reversal of myofibroblasts by amniotic membrane stromal extract
    Wei Li
    TissueTech, Inc, Miami, Florida, USA
    J Cell Physiol 215:657-64. 2008
    ..Further investigation into the identity of these factors and the control mechanisms may unravel a new scar-reversing strategy...
  65. ncbi Successful clinical implementation of corneal epithelial stem cell therapy for treatment of unilateral limbal stem cell deficiency
    Sai Kolli
    North East Institute for Stem Cell Research, International Centre for Life, NE1 3BZ, United Kingdom
    Stem Cells 28:597-610. 2010
    ....
  66. ncbi Smad5 determines murine amnion fate through the control of bone morphogenetic protein expression and signalling levels
    Erika A Bosman
    Department of Developmental Biology VIB7 for Biotechnology VIB and Laboratory of Molecular Biology Celgen, University of Leuven, B 3000 Leuven, Belgium
    Development 133:3399-409. 2006
    ..It is essential for primordial germ cell (PGC) development, for the development of the allantois and for amnion closure, as demonstrated by loss of Bmp signalling...
  67. pmc Exposure to in utero lipopolysaccharide induces inflammation in the fetal ovine skin
    Matthew W Kemp
    School of Women s and Infants Health, The University of Western Australia, Perth, Australia
    Reprod Sci 18:88-98. 2011
    ..These novel data strongly suggests that the fetal skin acts as an important mediator of the fetal inflammatory response and as such may contribute to preterm birth...
  68. ncbi Progesterone represses interleukin-8 and cyclo-oxygenase-2 in human lower segment fibroblast cells and amnion epithelial cells
    Jenifer A Z Loudon
    Imperial College Parturition Research Group, Wolfson and Weston Centre for Family Health, Institute of Reproductive and Developmental Biology, London W12 0HN, United Kingdom
    Biol Reprod 69:331-7. 2003
    ..and progesterone on IL-8 and prostaglandin E2 (PGE2) synthesis and IL-8 and COX-2 mRNA and promoter activity in amnion cells and lower segment fibroblast (LSF) cells...
  69. ncbi Amniotic mesenchymal tissue cells inhibit dendritic cell differentiation of peripheral blood and amnion resident monocytes
    Marta Magatti
    Centro di Ricerca E Menni, Fondazione Poliambulanza Istituto Ospedaliero, Brescia, Italy
    Cell Transplant 18:899-914. 2009
    ..We have previously shown that cells derived from the mesenchymal region of human amnion (AMTC) can strongly inhibit T-lymphocyte proliferation...
  70. doi Transplantation of human amniotic cells exerts neuroprotection in MPTP-induced Parkinson disease mice
    Xiang Ying Kong
    Department of Pharmacology, School of Basic Medicine, Peking Union Medical College and Institute of Basic Medical Science, Chinese Academy of Medical Sciences, Beijing, 100005 China
    Brain Res 1205:108-15. 2008
    ..The results suggested that transplanted HACs could survive and promote the endogenous neurogenesis of mice, which maybe related to the increased level of neurotrophins of the striatum...
  71. ncbi Association of PAT proteins with lipid storage droplets in term fetal membranes
    W E Ackerman
    Department of Obstetrics and Gynecology, Division of Maternal Fetal Medicine and Laboratory of Perinatal Research, The Ohio State University, College of Medicine, Columbus, OH 43210, USA
    Placenta 28:465-76. 2007
    ..We found that large LDs of amnion epithelial cells were reactive for neutral lipid stains and simultaneously encoated with ADRP and TIP47, but not ..
  72. pmc Characterization of immortalized human corneal endothelial cell line using HPV 16 E6/E7 on lyophilized human amniotic membrane
    Hyun Ju Kim
    Department of Ophthalmology, Chung Ang University Yongsan Hospital, Seoul, Korea
    Korean J Ophthalmol 20:47-54. 2006
    ..To establish the immortalized human corneal endothelial cell line (IHCEn) by transducing human papilloma virus (HPV) 16 E6/E7 oncogenes, and to identify their characteristics when cultivated on a lyophilized human amniotic membrane (LAM)...
  73. doi Rapid and efficient reprogramming of human amnion-derived cells into pluripotency by three factors OCT4/SOX2/NANOG
    Hong xi Zhao
    Department of Gynecology and Obstetrics, Tangdu Hospital, The Fourth Military Medical University, Xi an 710038, China
    Differentiation 80:123-9. 2010
    ..In this study, we tested whether human amnion-derived cells (hADCs) could be rapidly and efficiently reprogrammed into iPS cells by the defined factors: OCT4/..
  74. ncbi Transplanted human amniotic epithelial cells express connexin 26 and Na-K-adenosine triphosphatase in the inner ear
    Isamu Yuge
    Department of Otorhinolaryngology, Shinshu University School of Medicine, Asahi, Matsumoto, Japan
    Transplantation 77:1452-4. 2004
    ..This technology suggests the therapeutic potential for the treatment of hearing loss...
  75. doi Co-transplantation of bFGF-expressing amniotic epithelial cells and neural stem cells promotes functional recovery in spinal cord-injured rats
    Xiao ting Meng
    Department of Histology and Embryology, School of Bethune Medical Science, Jilin University, Changchun 130021, PR China
    Cell Biol Int 32:1546-58. 2008
    ..These findings suggest that a cograft of AECs/pLEGFP-hbFGF and NSCs may have benefits for SCI...
  76. doi Phenotypic shift of human amniotic epithelial cells in culture is associated with reduced osteogenic differentiation in vitro
    G Stadler
    Ludwig Boltzmann Institute for Experimental and Clinical Traumatology AUVA Research Center, Linz Vienna, Austria
    Cytotherapy 10:743-52. 2008
    ....
  77. doi Production of hepatocyte-like cells from human amnion
    Toshio Miki
    Departments of Pathology and Surgery and McGowan Institute for Regenerative Medicine, University of Pittsburgh, USA
    Methods Mol Biol 481:155-68. 2009
    ..Cells from the placental tissues such as amnion and chorion have been reported to display multilineage differentiation and surface marker and gene expression ..
  78. ncbi Human amniotic epithelial cells possess hepatocyte-like characteristics and functions
    Seiji Takashima
    Department of Organ Regeneration, Institutes of Organ Transplants, Reconstructive Medicine and Tissue Engineering, Shinshu University Graduate School of Medicine, Asahi, Matsumoto, Japan
    Cell Struct Funct 29:73-84. 2004
    ..In organ culture, the amnion secreted 30-fold larger amounts of albumin than human amniotic epithelial cells in monolayer culture...
  79. ncbi Role of glucocorticoid receptor and CCAAT/enhancer-binding protein alpha in the feed-forward induction of 11beta-hydroxysteroid dehydrogenase type 1 expression by cortisol in human amnion fibroblasts
    Zhen Yang
    School of Life Sciences, Fudan University, 220 Handan Road, Shanghai 200433, People s Republic of China
    J Endocrinol 195:241-53. 2007
    ..We examined the molecular mechanisms of the feedback effect of cortisol on 11beta-HSD1 mRNA expression in human amnion fibroblasts...
  80. ncbi Inducing proliferation of human amniotic epithelial (HAE) cells for cell therapy
    S Terada
    Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Fukui University, Japan
    Cell Transplant 9:701-4. 2000
    Probably because amnion is derived from the fetus and is exposed to the maternal immune system, human amniotic epithelial (HAE) cells do not express the HLA-A, -B, -C, or -DR antigens on their surfaces, suggesting that HAE cells do not ..
  81. pmc Immunosuppressive properties of human amniotic membrane for mixed lymphocyte reaction
    M Ueta
    Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan
    Clin Exp Immunol 129:464-70. 2002
    ..These results indicate that human AM has the ability to suppress allo-reactive T cells in vitro. This inhibitory effect likely contributes to the success of the ALT-AMT combination...
  82. ncbi Nuclear factor-kappa B is essential for up-regulation of interleukin-8 expression in human amnion and cervical epithelial cells
    C L Elliott
    Institute of Obstetrics and Gynaecology, Imperial College School of Medicine, Queen Charlotte s Hospital, Goldhawk Road, London W6 0XG, UK
    Mol Hum Reprod 7:787-90. 2001
    ..these sites to examine the relative importance of each site in IL-8 gene expression in a cervical cell line and in amnion cells obtained before and after labour...
  83. ncbi Apoptosis in the normal human amnion at term, independent of Bcl-2 regulation and onset of labour
    K Kumagai
    Department of Obstetrics and Gynecology, Osaka Medical College, 2-7 Daigaku-machi, Takatsuki, Osaka 569-8686, Japan
    Mol Hum Reprod 7:681-9. 2001
    This study was designed to detect apoptosis in the human amnion and to elucidate the signalling pathway involved in its regulation...
  84. ncbi IL-1beta and IL-8 in human fetal membranes: changes with gestational age, labor, and culture conditions
    C L Elliott
    Institute of Obstetrics and Gynaecology, Imperial College School of Medicine, London, UK
    Am J Reprod Immunol 46:260-7. 2001
    ..This study aimed to characterize their concentrations in fetal membranes and any changes in these with advancing gestation and to define as to whether there are interactions between the membranes in their expression...
  85. ncbi Interstitial collagen synthesis and processing in human amnion: a property of the mesenchymal cells
    M L Casey
    Cecil H and Ida Green Center for Reproductive Biology Sciences, University of Texas, Southwestern Medical Center, Dallas 75235, USA
    Biol Reprod 55:1253-60. 1996
    This study was conducted to evaluate the ontogeny and cellular site of interstitial collagen formation in amnion, the tissue that provides the tensile strength of the human fetal membranes...
  86. ncbi The human amnion is a site of MHC class Ib expression: evidence for the expression of HLA-E and HLA-G
    J M Houlihan
    University of Bristol, Department of Obstetrics and Gynecology, St Michael s Hospital, UK
    J Immunol 154:5665-74. 1995
    The expression of HLA class I Ag by term human amnion epithelial cells was investigated...
  87. pmc Protection of human amniotic epithelial cells (HAEC) from complement-mediated lysis: expression on the cells of three complement inhibitory membrane proteins
    I A Rooney
    Department of Medical Biochemistry, UWCM, Heath Park, Cardiff, U K
    Immunology 71:308-11. 1990
    ..Percentage cell lysis was markedly increased in the presence of Fab2 anti-CD59 and to a lesser, but significant, extent in the presence of Fab2 anti-DAF. Fab2 anti-MIP did not alter the sensitivity of the cells to lysis by complement...
  88. ncbi Biomechanical characterization of human amniotic membrane preparations for ocular surface reconstruction
    Roy S Chuck
    Department of Ophthalmology, University of California, Irvine, Calif, USA
    Ophthalmic Res 36:341-8. 2004
    ..To investigate the tensile and elastic properties of both commercially available and experimental human amniotic membrane preparations...
  89. ncbi Evidence of in vitro differential secretion of 72 and 92 kDa type IV collagenases after selective exposure to lipopolysaccharide in human fetal membranes
    Guadalupe Garcia-Lopez
    Direction of Research, Instituto Nacional de Perinatologia Isidro Espinosa de los Reyes, Mexico City 11000, Mexico
    Mol Hum Reprod 13:409-18. 2007
    ..the chorioamniotic membranes form two independent chambers, allowing for the selective stimulation of either the amnion (AMN) and/or the choriodecidua (CHD) regions...
  90. ncbi Toll-like receptor-2 and -4 in the chorioamniotic membranes in spontaneous labor at term and in preterm parturition that are associated with chorioamnionitis
    Yeon Mee Kim
    Perinatology Research Branch, National Institute of Child Health and Human Development NIH DHHS, Bethesda, MD, USA
    Am J Obstet Gynecol 191:1346-55. 2004
    ..This study examined Toll-like receptor-2 and -4 expression in fetal membranes in response to labor at term and histologic chorioamnionitis...
  91. doi On the origin of amniotic stem cells: of mice and men
    Mariya P Dobreva
    Laboratory of Developmental Signaling, Department of Molecular and Developmental Genetics, VIB, KU Leuven, Belgium
    Int J Dev Biol 54:761-77. 2010
    ..The amnion is the innermost extraembryonic membrane that eventually surrounds the fetus of amniotes, and contains the ..
  92. ncbi Inducing proliferation of human amnion epithelial and mesenchymal cells for prospective engineering of membrane repair
    Nicole Ochsenbein-Kölble
    Unit of Perinatal Physiology, Department of Obstetrics, University Hospital, Zurich, Switzerland
    J Perinat Med 31:287-94. 2003
    ..approach to fetal membrane repair after premature rupture of the membranes (PROM) by characterizing the proliferation potential of human amnion epithelial and mesenchymal cells from preterm and term placenta in primary culture.
  93. doi Fetal stem cells from extra-embryonic tissues: do not discard
    Akiva J Marcus
    The Ira B Black Center for Stem Cell Research and the Department of Neuroscience and Cell Biology, University of Medicine and Dentistry of New Jersey Robert Wood Johnson Medical School, Piscataway, NJ 08854 5635, USA
    J Cell Mol Med 12:730-42. 2008
    ..In this review we summarize recent work examining the plasticity and clinical potential of fetal stem cells isolated from extra-embryonic tissues...
  94. ncbi Immunogenicity and immunomodulatory effects of amnion-derived multipotent progenitor cells
    Richard Allan Banas
    Stemnion, Inc, Pittsburgh, PA, USA
    Hum Immunol 69:321-8. 2008
    This is the first study on the immunologic properties of a clinically relevant population of cells derived from the amnion of human placenta...
  95. ncbi Amniotic membrane transplantation for ocular surface reconstruction
    S C Tseng
    Ocular Surface and Tear Center, Bascom Palmer Eye Institute, William L McKnight Vision Research Pavillion, Miami, FL 33136, USA
    Biosci Rep 21:481-9. 2001
    The use of amniotic membrane (or amnion) for transplantation as graft in ocular surface reconstruction is reviewed...
  96. doi Amnion-derived cellular cytokine solution (ACCS) promotes migration of keratinocytes and fibroblasts
    M Georgina Uberti
    Institute for Tissue Regeneration, Repair, and Rehabilitation, Bay Pines VA Healthcare System, Bay Pines, FL, USA
    Ann Plast Surg 64:632-5. 2010
    b>Amnion-derived Multipotent Progenitor cells appear to be useful as adjuvants in wound healing...
  97. ncbi Topical application of culture supernatant from human amniotic epithelial cells suppresses inflammatory reactions in cornea
    Kazutaka Kamiya
    Department of Ophthalmology, University of Tokyo School of Medicine, Tokyo, Japan
    Exp Eye Res 80:671-9. 2005
    ..These results suggest that HAEC are a source of soluble anti-inflammatory factors and that conditioned medium from HAEC contains factors other than IL-1ra that suppress corneal inflammation...
  98. ncbi Reconstruction of ocular surface with heterologous limbal epithelium and amniotic membrane in a rabbit model
    M Avila
    Departamento de Oftalmologia, Facultad de Medicina, Hospital San Juan de Dios, Universidad Nacional de Colombia, Colombia
    Cornea 20:414-20. 2001
    ..To report in vivo reconstruction of the ocular surface using amniotic membrane and heterologous transplants of epithelial limbal cells in rabbits with chemical burns...
  99. ncbi Human amnion mesenchyme harbors cells with allogeneic T-cell suppression and stimulation capabilities
    Marta Magatti
    Centro di Ricerca E Menni, Fondazione Poliambulanza, Istituto Ospedaliero, Via Bissolati 57, I 25124 Brescia, Italy
    Stem Cells 26:182-92. 2008
    ..In this study, we isolated cells from the mesenchymal region of amnion and identified two subpopulations discordant for expression of the HLA-DR, CD45, CD14, and CD86 cellular markers...
  100. pmc Basement membrane dissolution and reassembly by limbal corneal epithelial cells expanded on amniotic membrane
    Wei Li
    Ocular Surface Center and TissueTech, Inc, Miami, Florida 33173, USA
    Invest Ophthalmol Vis Sci 47:2381-9. 2006
    ..To investigate basement membrane (BM) formation during ex vivo expansion of limbal corneal epithelial cells on intact amniotic membrane (iAM) and epithelially denuded (d)AM...
  101. ncbi Immunological characteristics of amniotic epithelium
    Junko Hori
    Department of Ophthalmology, Nippon Medical School, Sendagi 1 1 5, Bunkyo ku, Tokyo 113 8602, Japan
    Cornea 25:S53-8. 2006
    ..We therefore suggest that immunogenicity of AE should not be ignored, and use of AM from different donor placentas should be emphasized when repeated AM transplantation is required in patients clinically...

Research Grants88

  1. MECHANISMS OF FETAL MEMBRANE RUPTURE
    JEROME STRAUSS; Fiscal Year: 2009
    ..increases SERPINH1 promoter activity mitigating the effect of the 656 T allele;4) collagen synthesis is reduced in amnion fibroblasts carrying the 656 T allele, but not the 656 T allele in the presence of the 12 bp deletion;5) the ..
  2. MECHANISMS OF FETAL MEMBRANE RUPTURE
    Jerome F Strauss; Fiscal Year: 2010
    ..promoter activity mitigating the effect of the [unreadable]656 T allele;4) collagen synthesis is reduced in amnion fibroblasts carrying the [unreadable]656 T allele, but not the [unreadable]656 T allele in the presence of the 12 ..
  3. Genetics of preterm birth in African Americans
    JEROME STRAUSS; Fiscal Year: 2011
    ..contributing to preterm birth in African- Americans;2) the -656 T allele has diminished promoter activity in fetal amnion fibroblasts;3) a 12 bp deletion adjacent to the -656 T SNP opposes the adverse effect of the -656 T allele on ..
  4. Cellular Mechanisms of Amniotic Fluid Volume Regulation
    Cecilia Y Cheung; Fiscal Year: 2013
    ..These studies will be carried out in ovine amnion cells in vitro and chronically catheterized ovine fetuses in vivo...
  5. REGULATION OF PHOSPHOLIPASE A2 IN HUMAN AMNION
    Leslie Myatt; Fiscal Year: 2009
    The amnion is a major site of PGE2 production during labor and is a rich source of arachidonic acid, the precursor of PG production...
  6. Allogeneic Placental Stem Cells for Tissue Repair and Regeneration
    Vijay Kumar; Fiscal Year: 2010
    Discrete populations of stem cells reside within placental tissue (amnion, chorion, and umbilical cord matrix)...
  7. Repair of iatrogenic fetal membrane defects with an adhesive tissue scaffold
    Russell J Stewart; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Healthy and intact fetal membranes (amnion and chorion) are required for optimal pregnancy outcomes...
  8. Compartmental analysis of proteomic biomarkers during intra-uterine infections
    PETA GRIGSBY; Fiscal Year: 2009
    ..Moreover, these studies will provide important information on the contribution of the amnion or choriodecidua to the inflammatory response following U. parvum exposure...
  9. Compartmental analysis of proteomic biomarkers during intra-uterine infections
    PETA LOUISE GRIGSBY; Fiscal Year: 2010
    ..Moreover, these studies will provide important information on the contribution of the amnion or choriodecidua to the inflammatory response following U. parvum exposure...
  10. LIPOCORTINS AND ARACHIDONIC ACID RELEASE IN PARTURITION
    William Everson; Fiscal Year: 1991
    ..However, the intracellular events occurring in amnion and chorion which trigger this response are ill-defined...
  11. Cellular Mechanisms of Amniotic Fluid Volume Regulation
    Cecilia Y Cheung; Fiscal Year: 2010
    ..These studies will be carried out in ovine amnion cells in vitro and chronically catheterized ovine fetuses in vivo...
  12. REGULATION OF VEGF EXPRESSION IN PLACENTA AND MEMBRANES
    CECILIA CHEUNG; Fiscal Year: 2003
    ..Recently, we found expression of vascular endothelial growth factor (VEGF) in the amnion and chorion, and this expression is increased under conditions of enhanced intramembranous absorption...
  13. REGULATION OF VEGF EXPRESSION IN PLACENTA AND MEMBRANES
    CECILIA CHEUNG; Fiscal Year: 2004
    ..Recently, we found expression of vascular endothelial growth factor (VEGF) in the amnion and chorion, and this expression is increased under conditions of enhanced intramembranous absorption...
  14. REGULATION OF VEGF EXPRESSION IN PLACENTA AND MEMBRANES
    CECILIA CHEUNG; Fiscal Year: 2002
    ..Recently, we found expression of vascular endothelial growth factor (VEGF) in the amnion and chorion, and this expression is increased under conditions of enhanced intramembranous absorption...
  15. Mouse Model For Amniotic Fluid Volume Regulation
    CECILIA CHEUNG; Fiscal Year: 2001
    ..This pathway is constituted by a network of microvessels on the surface of the placenta and, in sheep, within the amnion and chorion as well...
  16. Mouse Model For Amniotic Fluid Volume Regulation
    CECILIA CHEUNG; Fiscal Year: 2002
    ..This pathway is constituted by a network of microvessels on the surface of the placenta and, in sheep, within the amnion and chorion as well...
  17. Allogeneic Placental Stem Cells for Tissue Repair and Regeneration
    Michael Friedman; Fiscal Year: 2009
    Discrete populations of stem cells reside within placental tissue (amnion, chorion, and umbilical cord matrix)...
  18. CATHEPSIN B-LIKE CYSTEINE PROTEINASES AND TUMOR INVASION
    Bonnie Sloane; Fiscal Year: 1991
    ..tumor cell degradation of the basement membrane or isolated components and/or 4) tumor cell invasion through the amnion basement membrane...
  19. CATHEPSIN B-LIKE CYSTEINE PROTEINASES AND TUMOR INVASION
    Bonnie Sloane; Fiscal Year: 1990
    ..tumor cell degradation of the basement membrane or isolated components and/or 4) tumor cell invasion through the amnion basement membrane...
  20. MECHANISMS OF FETAL MEMBRANE RUPTURE
    Jerome F Strauss; Fiscal Year: 2010
    ..increases SERPINH1 promoter activity mitigating the effect of the 656 T allele;4) collagen synthesis is reduced in amnion fibroblasts carrying the 656 T allele, but not the 656 T allele in the presence of the 12 bp deletion;5) the ..
  21. MECHANISMS OF FETAL MEMBRANE RUPTURE
    JEROME STRAUSS; Fiscal Year: 2009
    ..increases SERPINH1 promoter activity mitigating the effect of the 656 T allele;4) collagen synthesis is reduced in amnion fibroblasts carrying the 656 T allele, but not the 656 T allele in the presence of the 12 bp deletion;5) the ..
  22. LOCAL AND SYSTEMIC CONSEQUENCES OF ISCHEMIA
    HERBERT HECHTMAN; Fiscal Year: 1990
    ..In vitro methods using pulmonary artery and pulmonary microvessel endothelial cells (EC) grown on an amnion membrane or microcarrier beads will be used to study mechanisms of PMN diapedesis and EC permeability...
  23. Fetal Membrane AQP Expression/Amniotic Fluid Regulation
    Michael Ross; Fiscal Year: 2003
    ..In situ hybridization reveals that AQP8 is expressed in epithelial cells of human amnion and chorion, as well as trophoblasts of placenta...
  24. Fetal Membrane AQP Expression/Amniotic Fluid Regulation
    Michael Ross; Fiscal Year: 2004
    ..In situ hybridization reveals that AQP8 is expressed in epithelial cells of human amnion and chorion, as well as trophoblasts of placenta...
  25. ENDOCRINE AND VASCULAR RELATIONSHIPS IN PARTURITION
    MILES NOVY; Fiscal Year: 1993
    ..rates in a tissue superfusion system; PG inhibitory and stimulatory substances by bovine seminal vesicles and amnion cell cultures...
  26. ENDOCRINE AND VASCULAR RELATIONSHIPS IN PARTURITION
    MILES NOVY; Fiscal Year: 1990
    ..rates in a tissue superfusion system; PG inhibitory and stimulatory substances by bovine seminal vesicles and amnion cell cultures...
  27. ENDOCRINE AND VASCULAR RELATIONSHIPS IN PARTURITION
    MILES NOVY; Fiscal Year: 1991
    ..rates in a tissue superfusion system; PG inhibitory and stimulatory substances by bovine seminal vesicles and amnion cell cultures...
  28. ENDOCRINE AND VASCULAR RELATIONSHIPS IN PARTURITION
    MILES NOVY; Fiscal Year: 1992
    ..rates in a tissue superfusion system; PG inhibitory and stimulatory substances by bovine seminal vesicles and amnion cell cultures...
  29. ROLE OF MONONUCLEAR LEUKOCYTES IN IMMUNITY
    Samuel Silverstein; Fiscal Year: 1992
    ..We have developed a "model" vessel wall composed of bovine endothelial cells cultured on human amnion. The endothelial cell layer is "tight" to macromolecular tracers...
  30. SIGNAL TRANSDUCTION MECHANISMS AND AMNION PGE2 SYNTHESIS
    Leslie Myatt; Fiscal Year: 1990
    Synthesis and release of eicosanoids by amnion in response to a fetal or maternal signal may be involved the onset of parturition in humans but the intracellular signal transduction mechanisms that lead to eicosanoid synthesis (the ..
  31. INITIATION OF HUMAN LABOR--PREVENTION OF PREMATURITY
    M Casey; Fiscal Year: 1993
    ..appearance of uterine phase 1 proteins in myometrium; and, (iii) a newly discovered biological system of the fetal amnion, i.e., the formation of a vasocontractant and a vasorelaxant that can be transported to the chorionic vessels...
  32. INHIBITION OF UTERINE CONTRACTIONS BY FETAL MEMBRANES
    Patricia Collins; Fiscal Year: 1999
    ..and that the inhibitory effect is specific to the chorion/decidual side because membranes mounted with the amnion side toward the muscle did not show inhibition...
  33. The Molecular Pathogenesis of Health Disparities in Inf*
    Emmet Hirsch; Fiscal Year: 2004
    ..In the second phase of the project, we analyze human tissues (myometrium, placenta, chorion, amnion, decidua, amniocytes and blood) collected from approximately 1360 patients presenting in term and preterm labor in ..
  34. The Molecular Pathogenesis of Health Disparities in Inf*
    Emmet Hirsch; Fiscal Year: 2002
    ..In the second phase of the project, we analyze human tissues (myometrium, placenta, chorion, amnion, decidua, amniocytes and blood) collected from approximately 1360 patients presenting in term and preterm labor in ..
  35. The Molecular Pathogenesis of Health Disparities in Inf*
    Emmet Hirsch; Fiscal Year: 2005
    ..In the second phase of the project, we analyze human tissues (myometrium, placenta, chorion, amnion, decidua, amniocytes and blood) collected from approximately 1360 patients presenting in term and preterm labor in ..
  36. The Molecular Pathogenesis of Health Disparities in Inf*
    Emmet Hirsch; Fiscal Year: 2003
    ..In the second phase of the project, we analyze human tissues (myometrium, placenta, chorion, amnion, decidua, amniocytes and blood) collected from approximately 1360 patients presenting in term and preterm labor in ..
  37. PATHOGENESIS OF HEALTH DISPARITIES IN PRETERM BIRTH
    Emmet Hirsch; Fiscal Year: 2001
    ..In the second phase of the project, we analyze human tissues (myometrium, placenta, chorion, amnion, decidua, amniocytes and blood) collected from approximately 1360 patients presenting in term and preterm labor in ..
  38. REGULATION OF PHOSPHOLIPASE A2 IN HUMAN AMNION
    Leslie Myatt; Fiscal Year: 2005
    The amnion is a major site of PGE2 production during labor and is a rich source of arachidonic acid, the precursor of PG production...
  39. FETAL MEMBRANE CELL INTERACTIONS, ANATOMICAL RELATIONSHIPS, AND FUNCTIONS
    Judith Head; Fiscal Year: 2002
    ..are coordinated with those designed to define the ontogeny of interstitial pro-collagen synthesis/processing in amnion. An alternative approach to most previous studies is proposed; viz, an investigation of the region of the fetal ..
  40. REGULATION OF PHOSPHOLIPASE A2 IN HUMAN AMNION
    Leslie Myatt; Fiscal Year: 2007
    The amnion is a major site of PGE2 production during labor and is a rich source of arachidonic acid, the precursor of PG production...
  41. REGULATION OF PHOSPHOLIPASE A2 IN HUMAN AMNION
    Leslie Myatt; Fiscal Year: 2009
    The amnion is a major site of PGE2 production during labor and is a rich source of arachidonic acid, the precursor of PG production...
  42. ROLE OF MONONUCLEAR LEUKOCYTES IN IMMUNITY
    Samuel Silverstein; Fiscal Year: 1991
    ..We have developed a "model" vessel wall composed of bovine endothelial cells cultured on human amnion. The endothelial cell layer is "tight" to macromolecular tracers...
  43. ROLE OF MONONUCLEAR LEUKOCYTES IN IMMUNITY
    Samuel Silverstein; Fiscal Year: 1990
    ..We have developed a "model" vessel wall composed of bovine endothelial cells cultured on human amnion. The endothelial cell layer is "tight" to macromolecular tracers...
  44. OXIDATIVE DAMAGE AND PREMATURE RUPTURE OF THE MEMBRANES
    James Woods; Fiscal Year: 2004
    ..laboratory demonstrate that, in physiologic concentrations, the ROS, hypochlorous acid and superoxide, do damage amnion epithelium and collagen I, the strongest component of chorioamnion...
  45. OXIDATIVE DAMAGE AND PREMATURE RUPTURE OF THE MEMBRANES
    James Woods; Fiscal Year: 2003
    ..laboratory demonstrate that, in physiologic concentrations, the ROS, hypochlorous acid and superoxide, do damage amnion epithelium and collagen I, the strongest component of chorioamnion...
  46. OXIDATIVE DAMAGE AND PREMATURE RUPTURE OF THE MEMBRANES
    James Woods; Fiscal Year: 2002
    ..laboratory demonstrate that, in physiologic concentrations, the ROS, hypochlorous acid and superoxide, do damage amnion epithelium and collagen I, the strongest component of chorioamnion...
  47. OXIDATIVE DAMAGE AND PREMATURE RUPTURE OF THE MEMBRANES
    James Woods; Fiscal Year: 2001
    ..laboratory demonstrate that, in physiologic concentrations, the ROS, hypochlorous acid and superoxide, do damage amnion epithelium and collagen I, the strongest component of chorioamnion...
  48. INFECTION AND HISTOPATHOLOGY IN PRETERM LABOR
    David Eschenbach; Fiscal Year: 1993
    ..Organisms can be isolated from between the chorion and amnion of 60% of the women who deliver prematurely, in contrast to only 20% of women who deliver at term...
  49. INFECTION AND HISTOPATHOLOGY IN PRETERM LABOR
    David Eschenbach; Fiscal Year: 1991
    ..Organisms can be isolated from between the chorion and amnion of 60% of the women who deliver prematurely, in contrast to only 20% of women who deliver at term...
  50. INFECTION AND HISTOPATHOLOGY IN PRETERM LABOR
    David Eschenbach; Fiscal Year: 1992
    ..Organisms can be isolated from between the chorion and amnion of 60% of the women who deliver prematurely, in contrast to only 20% of women who deliver at term...
  51. Progesterone Receptor Function in Initiation of Labor
    CAROLE MENDELSON; Fiscal Year: 2002
    ..is effected at the cellular/gene level; (iii) define the ontogeny/regulation of interstitial collagen formation in amnion and the impact of selected risk factors for pre-term pre-mature rupture of the fetal membranes (PT-PROM) on pro-..
  52. OXYTOCIN-RECEPTOR-MEDIATED PROSTAGLANDIN RELEASE
    MELVYN SOLOFF; Fiscal Year: 1990
    ..proposal is to determine the relationship between the occupancy of OT bindi g sites in decidua, endometrium and amnion and the production of PGs. The specific aims are to: 1...
  53. OXYTOCIN-RECEPTOR-MEDIATED PROSTAGLANDIN RELEASE
    MELVYN SOLOFF; Fiscal Year: 1993
    ..proposal is to determine the relationship between the occupancy of OT binding sites in decidua, endometrium and amnion and the production of PGs. The specific aims are to: 1...
  54. OXYTOCIN-RECEPTOR-MEDIATED PROSTAGLANDIN RELEASE
    MELVYN SOLOFF; Fiscal Year: 1991
    ..proposal is to determine the relationship between the occupancy of OT binding sites in decidua, endometrium and amnion and the production of PGs. The specific aims are to: 1...
  55. REGULATION OF PHOSPHOLIPASE A2 IN HUMAN AMNION
    Leslie Myatt; Fiscal Year: 2006
    The amnion is a major site of PGE2 production during labor and is a rich source of arachidonic acid, the precursor of PG production...
  56. ENDOCRINE AND PARACRINE RELATIONSHIPS IN PARTURITION
    MILES NOVY; Fiscal Year: 2002
    ..Aim 2 will describe the presence of androgen and glucocorticoid receptors (ARs, GRs) in rhesus amnion, chorion, decidua, and myometrium at 80 and 100 percent of gestation using immunohistochemistry and ribonuclease ..
  57. In-ovo vaccination/avian influenza/bird/human
    DE CHU TANG; Fiscal Year: 2006
    ..Both vaccines will be injected into the amnion of chicken embryos...
  58. ENDOCRINE AND PARACRINE RELATIONSHIPS IN PARTURITION
    MILES NOVY; Fiscal Year: 2000
    ..Aim 2 will describe the presence of androgen and glucocorticoid receptors (ARs, GRs) in rhesus amnion, chorion, decidua, and myometrium at 80 and 100 percent of gestation using immunohistochemistry and ribonuclease ..
  59. BIOMOLECULAR BASIS OF INFECTION-INDUCED PRETERM LABOR
    Susan Cox; Fiscal Year: 1990
    ..evaluate the mechanisms that are operative in the expression of receptors for cytokines in decidua, chorion leave, amnion, and villous trophoblastic tissues...
  60. HYPOXIA EFFECTS ON AMNIOTIC FLUID VOLUME
    ROBERT BRACE; Fiscal Year: 2007
    ..pathway will be determined by studying the effects of VEGF on transport in isolated amniotic membrane and amnion cells in culture...
  61. HYPOXIA EFFECTS ON AMNIOTIC FLUID VOLUME
    ROBERT BRACE; Fiscal Year: 2006
    ..pathway will be determined by studying the effects of VEGF on transport in isolated amniotic membrane and amnion cells in culture...
  62. HYPOXIA EFFECTS ON AMNIOTIC FLUID VOLUME
    ROBERT BRACE; Fiscal Year: 2005
    ..pathway will be determined by studying the effects of VEGF on transport in isolated amniotic membrane and amnion cells in culture...
  63. HYPOXIA EFFECTS ON AMNIOTIC FLUID VOLUME
    ROBERT BRACE; Fiscal Year: 2004
    ..pathway will be determined by studying the effects of VEGF on transport in isolated amniotic membrane and amnion cells in culture...
  64. GLUCOCORTICOIDS AND MATRIX PROTEINS AT PARTURITION
    Seth Guller; Fiscal Year: 1999
    ..completely suppress the synthesis of FN and other ECM proteins in primary cultures of human cytotrophoblasts and amnion epithelial cells...
  65. INITIATION OF HUMAN LABOR: PREVENTION OF PREMATURITY
    CAROLE MENDELSON; Fiscal Year: 2002
    ..is effected at the cellular/gene level; (iii) define the ontogeny/regulation of interstitial collagen formation in amnion and the impact of selected risk factors for pre-term pre-mature rupture of the fetal membranes (PT-PROM) on pro-..
  66. INITIATION OF HUMAN LABOR: PREVENTION OF PREMATURITY
    CAROLE MENDELSON; Fiscal Year: 2001
    ..is effected at the cellular/gene level; (iii) define the ontogeny/regulation of interstitial collagen formation in amnion and the impact of selected risk factors for pre-term pre-mature rupture of the fetal membranes (PT-PROM) on pro-..
  67. MECHANISMS OF FETAL MEMBRANE RUPTURE
    JEROME STRAUSS; Fiscal Year: 2004
    ..We will determine whether proteolytic fragments of fibronectin induce MMP expression in human amnion cells in vitro; define the receptor(s) and signal transduction cascade underlying this response; identify active ..
  68. INITIATION OF HUMAN LABOR: PREVENTION OF PREMATURITY
    M Casey; Fiscal Year: 1999
    ..is effected at the cellular/gene level; (iii) define the ontogeny/regulation of interstitial collagen formation in amnion and the impact of selected risk factors for pre-term pre-mature rupture of the fetal membranes (PT-PROM) on pro-..
  69. ROLE OF INTERLEUKIN-6 IN PRETERM LABOR
    Donald Dudley; Fiscal Year: 1991
    ..gestational tissues in response to infection and that IL-6 will stimulate prostaglandin production by decidua and amnion; (2) the regulation of IL-t production is mediated by other inflammatory cytokines, effectors of cell signal ..
  70. MECHANISMS OF FETAL MEMBRANE RUPTURE
    JEROME STRAUSS; Fiscal Year: 2001
    ..We will determine whether proteolytic fragments of fibronectin induce MMP expression in human amnion cells in vitro; define the receptor(s) and signal transduction cascade underlying this response; identify active ..
  71. INITIATION OF HUMAN LABOR: PREVENTION OF PREMATURITY
    CAROLE MENDELSON; Fiscal Year: 2000
    ..is effected at the cellular/gene level; (iii) define the ontogeny/regulation of interstitial collagen formation in amnion and the impact of selected risk factors for pre-term pre-mature rupture of the fetal membranes (PT-PROM) on pro-..
  72. MECHANISMS OF FETAL MEMBRANE RUPTURE
    JEROME STRAUSS; Fiscal Year: 2002
    ..We will determine whether proteolytic fragments of fibronectin induce MMP expression in human amnion cells in vitro; define the receptor(s) and signal transduction cascade underlying this response; identify active ..
  73. ENDOTHELIAL CELL COMMUNICATION IN HOMEOSTASIS
    David Shepro; Fiscal Year: 1990
    ..treatment with barrier-mediating agents, cultures will be grown on microcarrier beads or on human acellular amnion membranes. These cultures provide a two-compartment assay to measure cell and macromolecule transit...
  74. MECHANISMS OF FETAL MEMBRANE RUPTURE
    JEROME STRAUSS; Fiscal Year: 2003
    ..We will determine whether proteolytic fragments of fibronectin induce MMP expression in human amnion cells in vitro; define the receptor(s) and signal transduction cascade underlying this response; identify active ..
  75. MECHANISMS OF FETAL MEMBRANE RUPTURE
    JEROME STRAUSS; Fiscal Year: 2005
    ..We will determine whether proteolytic fragments of fibronectin induce MMP expression in human amnion cells in vitro; define the receptor(s) and signal transduction cascade underlying this response; identify active ..