amnion

Summary

Summary: The innermost membranous sac that surrounds and protects the developing EMBRYO which is bathed in the AMNIOTIC FLUID. Amnion cells are secretory EPITHELIAL CELLS and contribute to the amniotic fluid.

Top Publications

  1. ncbi Concise review: isolation and characterization of cells from human term placenta: outcome of the first international Workshop on Placenta Derived Stem Cells
    Ornella Parolini
    Centro di Ricerca E Menni, Fondazione Poliambulanza, Istituto Ospedaliero, Via Bissolati 57, 25124 Brescia, Italy
    Stem Cells 26:300-11. 2008
  2. ncbi Engraftment potential of human amnion and chorion cells derived from term placenta
    Marco Bailo
    Centro Ricerche Parco Scientifico E Menni, Ospedale Poliambulanza, Via Romiglia, 4, I 25124 Brescia, Italy
    Transplantation 78:1439-48. 2004
  3. ncbi Mice deficient for BMP2 are nonviable and have defects in amnion/chorion and cardiac development
    H Zhang
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA
    Development 122:2977-86. 1996
  4. ncbi The role of inflammation and infection in preterm birth
    Roberto Romero
    Perinatology Research Branch, National Institute of Child Health and Human Development, National Institutes of Health, Department of Health and Human Services, Bethesda, Maryland, USA
    Semin Reprod Med 25:21-39. 2007
  5. ncbi Human amnion epithelial cell transplantation abrogates lung fibrosis and augments repair
    Yuben Moodley
    F R A C P School of Medicine and Pharmacology, University of Western Australia, 50 Murray Street, Perth, Western Australia 6000, Australia
    Am J Respir Crit Care Med 182:643-51. 2010
  6. ncbi Stem cell characteristics of amniotic epithelial cells
    Toshio Miki
    Department of Pathology and McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA
    Stem Cells 23:1549-59. 2005
  7. ncbi Stem cells derived from human fetal membranes display multilineage differentiation potential
    Sivakami Ilancheran
    Center for Women s Health Research, Department of Obstetrics and Gynecology, Monash University, Monash Medical Center, Clayton, Victoria 3168, Australia
    Biol Reprod 77:577-88. 2007
  8. ncbi Inflammation in preterm and term labour and delivery
    Roberto Romero
    Perinatology Research Branch, NICHD, NIH, DHHS, Bethesda, MD, USA
    Semin Fetal Neonatal Med 11:317-26. 2006
  9. ncbi Amniotic membrane and amniotic cells: potential therapeutic tools to combat tissue inflammation and fibrosis?
    U Manuelpillai
    Monash Institute of Medical Research, Monash University, Clayton, Victoria 3168, Australia
    Placenta 32:S320-5. 2011
  10. ncbi Amnion: a potent graft source for cell therapy in stroke
    Seong Jin Yu
    Department of Neurology, Medical College of Georgia, Augusta, GA, USA
    Cell Transplant 18:111-8. 2009

Detail Information

Publications324 found, 100 shown here

  1. ncbi Concise review: isolation and characterization of cells from human term placenta: outcome of the first international Workshop on Placenta Derived Stem Cells
    Ornella Parolini
    Centro di Ricerca E Menni, Fondazione Poliambulanza, Istituto Ospedaliero, Via Bissolati 57, 25124 Brescia, Italy
    Stem Cells 26:300-11. 2008
    ....
  2. ncbi Engraftment potential of human amnion and chorion cells derived from term placenta
    Marco Bailo
    Centro Ricerche Parco Scientifico E Menni, Ospedale Poliambulanza, Via Romiglia, 4, I 25124 Brescia, Italy
    Transplantation 78:1439-48. 2004
    ..which may entail progenitor potential. The immunologic reactivity and the transplantation potential of amnion and chorion cells, however, remain to be elucidated.
  3. ncbi Mice deficient for BMP2 are nonviable and have defects in amnion/chorion and cardiac development
    H Zhang
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA
    Development 122:2977-86. 1996
    ..Mutant embryos failed to close the proamniotic canal, which caused the malformation of the amnion/chorion...
  4. ncbi The role of inflammation and infection in preterm birth
    Roberto Romero
    Perinatology Research Branch, National Institute of Child Health and Human Development, National Institutes of Health, Department of Health and Human Services, Bethesda, Maryland, USA
    Semin Reprod Med 25:21-39. 2007
    ..This article reviews the role of inflammation in preterm and term parturition...
  5. ncbi Human amnion epithelial cell transplantation abrogates lung fibrosis and augments repair
    Yuben Moodley
    F R A C P School of Medicine and Pharmacology, University of Western Australia, 50 Murray Street, Perth, Western Australia 6000, Australia
    Am J Respir Crit Care Med 182:643-51. 2010
    ..Cellular therapies that could restore pneumocytes and reduce inflammation and fibrosis would be a major advance in management...
  6. ncbi Stem cell characteristics of amniotic epithelial cells
    Toshio Miki
    Department of Pathology and McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA
    Stem Cells 23:1549-59. 2005
    ..b>Amnion derived from term placenta after live birth may be a useful and noncontroversial source of stem cells for cell ..
  7. ncbi Stem cells derived from human fetal membranes display multilineage differentiation potential
    Sivakami Ilancheran
    Center for Women s Health Research, Department of Obstetrics and Gynecology, Monash University, Monash Medical Center, Clayton, Victoria 3168, Australia
    Biol Reprod 77:577-88. 2007
    The amnion is the inner of two membranes surrounding the fetus. That it arises from embryonic epiblast cells prior to gastrulation suggests that it may retain a reservoir of stem cells throughout pregnancy...
  8. ncbi Inflammation in preterm and term labour and delivery
    Roberto Romero
    Perinatology Research Branch, NICHD, NIH, DHHS, Bethesda, MD, USA
    Semin Fetal Neonatal Med 11:317-26. 2006
    ..It is possible that modulation of inflammation using anti-inflammatory cytokines, corticoids, antioxidants and/or other factors may complement antibiotic therapy and limit fetal injury...
  9. ncbi Amniotic membrane and amniotic cells: potential therapeutic tools to combat tissue inflammation and fibrosis?
    U Manuelpillai
    Monash Institute of Medical Research, Monash University, Clayton, Victoria 3168, Australia
    Placenta 32:S320-5. 2011
    ....
  10. ncbi Amnion: a potent graft source for cell therapy in stroke
    Seong Jin Yu
    Department of Neurology, Medical College of Georgia, Augusta, GA, USA
    Cell Transplant 18:111-8. 2009
    ..Recently, amnion-derived cells have been reported to have multipotent differentiation ability, and these cells have attracted ..
  11. ncbi Dose-dependent immunomodulatory effect of human stem cells from amniotic membrane: a comparison with human mesenchymal stem cells from adipose tissue
    Susanne Wolbank
    Red Cross Blood Transfusion Service of Upper Austria, Linz, Austria
    Tissue Eng 13:1173-83. 2007
    ..All three cell types may be considered as possible alternatives to BMSCs for allogeneic application in tissue engineering...
  12. pmc Critical appraisal of ex vivo expansion of human limbal epithelial stem cells
    S C G Tseng
    Research and Development Department, Tissue Tech, Inc, and Ocular Surface Center Miami, FL, USA
    Curr Mol Med 10:841-50. 2010
    ..Such an approach may one day help realize considerable promise held by adult SCs in treating a number of diseases...
  13. ncbi Suppression of transforming growth factor-beta isoforms, TGF-beta receptor type II, and myofibroblast differentiation in cultured human corneal and limbal fibroblasts by amniotic membrane matrix
    S C Tseng
    Ocular Surface and Tear Center, Department of Ophthalmology, Bascom Palmer Eye Institute, University of Miami School of Medicine, Florida, USA
    J Cell Physiol 179:325-35. 1999
    ..It may also explain in part why fetal wound healing is scarless...
  14. ncbi Propolis and amnion reepithelialise second-degree burns in rats
    Alicia Greyce Turatti Pessolato
    Faculty of Veterinary Medicine and Animal Science, Department of Surgery, University of Sao Paulo, São Paulo 05508 270, Sao Paulo, Brazil
    Burns 37:1192-201. 2011
    ..repair or healing of burns in rats that were treated either daily with 5% propolis ointment or by autologous amnion graft...
  15. ncbi Immunosuppressive factors secreted by human amniotic epithelial cells
    Haochuan Li
    Department of Ophthalmology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    Invest Ophthalmol Vis Sci 46:900-7. 2005
    ....
  16. ncbi Isolation, characterization, and differentiation of stem cells derived from the rat amniotic membrane
    Akiva J Marcus
    The Ira B Black Center for Stem Cell Research, Department of Neuroscience and Cell Biology, University of Medicine and Dentistry of New Jersey Robert Wood Johnson Medical School, Piscataway, NJ 08854 5635, USA
    Differentiation 76:130-44. 2008
    ..Similar to mesenchymal stem cells, these amnion-derived stem cells (ADSCs) express the surface markers CD29 and CD90, but were negative for the ..
  17. ncbi Comparative characterization of cultured human term amnion epithelial and mesenchymal stromal cells for application in cell therapy
    Grozdana Bilic
    Department of Obstetrics, University Hospital Zurich, Zurich, Switzerland
    Cell Transplant 17:955-68. 2008
    Emerging evidence suggests human amnion tissue as a valuable source of two distinct types of pluripotent cells, amnion epithelial cells (hAECs) and mesenchymal stromal cells (hAMSCs), for applications in cell replacement therapy...
  18. ncbi Transplantation of allogeneic and xenogeneic placenta-derived cells reduces bleomycin-induced lung fibrosis
    Anna Cargnoni
    Centro di Ricerca E Menni, Fondazione Poliambulanza Istituto Ospedaliero, 25124 Brescia, Italy
    Cell Transplant 18:405-22. 2009
    Fetal membranes (amnion and chorion) have recently raised significant attention as potential sources of stem cells...
  19. ncbi Clinical outcomes of xeno-free autologous cultivated limbal epithelial transplantation: a 10-year study
    Virender S Sangwan
    Cornea and Anterior Segment Services, L V Prasad Eye Institute, Kallam Anji Reddy Campus, Road No 2, Banjara Hills, Hyderabad 500034, India
    Br J Ophthalmol 95:1525-9. 2011
    ..Ocular burns can damage the corneal epithelial stem cells located at the limbus. This study evaluated the efficacy of xeno-free autologous cell-based treatment of limbal stem cell deficiency...
  20. ncbi Growth factor mRNA and protein in preserved human amniotic membrane
    N J Koizumi
    Department of Ophthalmology, Kyoto Prefectural University of Medicine, Japan
    Curr Eye Res 20:173-7. 2000
    ..To investigate the expression of growth factor mRNA and the level of growth factor protein in preserved human amniotic membrane (AM)...
  21. pmc Transplantation of cultivated autologous oral mucosal epithelial cells in patients with severe ocular surface disorders
    T Nakamura
    Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kawaramachi Hirokoji, Kamigyo ku, Kyoto 602 0841 Japan
    Br J Ophthalmol 88:1280-4. 2004
    ..To determine outcomes of transplants of cultivated autologous oral epithelial cells in patients with severe ocular surface disorders...
  22. ncbi Role of human amniotic epithelial cell transplantation in spinal cord injury repair research
    V Sankar
    Department of Anatomy, Dr Arcot Lakshmanaswamy Mudaliar Postgraduate Institute of Basic Medical Sciences, Taramani Campus, University of Madras, 600 113, Chennai, India
    Neuroscience 118:11-7. 2003
    ..First, there was no evidence of immunological rejection probably due to the non-antigenic nature of the HAEC. Second, unlike neural tissue, procurement of HAEC does not involve many legal or ethical problems...
  23. ncbi BMP-dependent serosa and amnion specification in the scuttle fly Megaselia abdita
    Ab Matteen Rafiqi
    University of Chicago, Department of Organismal Biology and Anatomy, Chicago, IL 60637, USA
    Development 139:3373-82. 2012
    ..extra-embryonic tissue specification, we examined the activity profile and function of BMP signaling in serosa and amnion patterning of the scuttle fly Megaselia abdita (Phoridae) and compared the BMP activity profiles between M...
  24. ncbi Production of an acellular amniotic membrane matrix for use in tissue engineering
    Stacy Paul Wilshaw
    Institute of Medical and Biological Engineering, University of Leeds, Leeds, United Kingdom
    Tissue Eng 12:2117-29. 2006
    ..A novel detergent-based protocol was modified to remove all cellular components from amnion to render it non-immunogenic. Amnion was harvested within 24 h after elective caesarean section (n = 12)...
  25. ncbi Fetal stem cells from extra-embryonic tissues: do not discard
    Akiva J Marcus
    The Ira B Black Center for Stem Cell Research and the Department of Neuroscience and Cell Biology, University of Medicine and Dentistry of New Jersey Robert Wood Johnson Medical School, Piscataway, NJ 08854 5635, USA
    J Cell Mol Med 12:730-42. 2008
    ..In this review we summarize recent work examining the plasticity and clinical potential of fetal stem cells isolated from extra-embryonic tissues...
  26. ncbi Regulation of phospholipase isozymes by nuclear factor-kappaB in human gestational tissues in vitro
    Martha Lappas
    Department of Obstetrics and Gynaecology, The University of Melbourne and Mercy Perinatal Research Centre, Mercy Hospital for Women, East Melbourne, Victoria, Australia 3002
    J Clin Endocrinol Metab 89:2365-72. 2004
    ..Human placenta, amnion, and choriodecidua (n = 4-9 separate placentas) were incubated in the presence of SASP (0...
  27. ncbi Outcomes and DNA analysis of ex vivo expanded stem cell allograft for ocular surface reconstruction
    Sheraz M Daya
    Corneo Plastic Unit, Queen Victoria Hospital, East Grinstead, West Sussex, United Kingdom
    Ophthalmology 112:470-7. 2005
    ..To investigate the outcome of a new technique of ex vivo expanded stem cell allograft for limbal stem cell deficiency (LSCD), and to characterize the ocular surface genotype after surgery...
  28. pmc The effects of thrombin and cytokines upon the biomechanics and remodeling of isolated amnion membrane, in vitro
    D Kumar
    Department of Pediatrics, Case Western Reserve University, Cleveland, OH, USA
    Placenta 32:206-13. 2011
    ..The purpose of these studies was to determine whether thrombin and cytokines directly weaken the amnion membrane (AM), the major load-bearing component of FM...
  29. ncbi Standardized limbal epithelial stem cell graft generation and transplantation
    Nadia Zakaria
    Department of Ophthalmology, University Hospital Antwerp, Antwerp, Belgium
    Tissue Eng Part C Methods 16:921-7. 2010
    ..To describe a standardized, xenogenic-free protocol for the manufacture of limbal epithelial stem cell grafts and a "no touch" surgical technique for its standardized transplantation...
  30. ncbi Titanium dioxide nanoparticles induced cytotoxicity, oxidative stress and DNA damage in human amnion epithelial (WISH) cells
    Quaiser Saquib
    Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia
    Toxicol In Vitro 26:351-61. 2012
    Titanium dioxide nanoparticles (TiO(2)-NPs) induced cytotoxicity and DNA damage have been investigated using human amnion epithelial (WISH) cells, as an in vitro model for nanotoxicity assessment...
  31. ncbi Human amnion-derived mesenchymal stem cells are a potential source for uterine stem cell therapy
    K Han
    Department of Biomedical Science and Technology, Research Center for Drugs, Institute of Biomedical Science and Technology, Konkuk University, Seoul, South Korea
    Cell Prolif 41:709-25. 2008
    Human amnion is an easy-to-obtain novel source of human mesenchymal stem cells, which poses little or no ethical dilemmas...
  32. pmc Expression patterns of microRNAs in the chorioamniotic membranes: a role for microRNAs in human pregnancy and parturition
    D Montenegro
    Perinatology Research Branch, NICHD NIH DHHS, Bethesda, Maryland, USA
    J Pathol 217:113-21. 2009
    ..Furthermore, the expression level of Dicer in the chorioamniotic membranes dichotomizes pathological preterm labour and physiological spontaneous labour at term...
  33. ncbi Midterm results on ocular surface reconstruction using cultivated autologous oral mucosal epithelial transplantation
    Tsutomu Inatomi
    Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan
    Am J Ophthalmol 141:267-275. 2006
    ..To perform a midterm assessment of the integrity and reproducibility of cultivated autologous oral mucosal epithelial sheets, and to evaluate the clinical efficacy of their transplantation in ocular surface...
  34. ncbi Optimization of amniotic membrane (AM) denuding for tissue engineering
    Andrew Hopkinson
    Division of Ophthalmology and Visual Sciences, University of Nottingham, Nottingham, United Kingdom
    Tissue Eng Part C Methods 14:371-81. 2008
    ..We compare the effects of the main denuding procedures, and propose a highly effective standardized alternative...
  35. pmc Pulmonary and systemic inflammatory responses to intra-amniotic IL-1α in fetal sheep
    Suhas G Kallapur
    Cincinnati Children s Hospital Medical Center, Univ of Cincinnati, Division of Pulmonary Biology, 3333 Burnet Ave, Cincinnati, OH 45229 3039, USA
    Am J Physiol Lung Cell Mol Physiol 301:L285-95. 2011
    ..IL-1α is a potent mediator of the fetal inflammatory response syndrome...
  36. ncbi Antiinflammatory effects of amniotic membrane transplantation in ocular surface disorders
    S Shimmura
    Department of Ophthalmology, Tokyo Dental College, Tokyo, Japan
    Cornea 20:408-13. 2001
    ..CONCLUSION: Amniotic membrane attracts and traps inflammatory cells infiltrating the ocular surface, which may explain some of the antiinflammatory properties of the fetal tissue...
  37. pmc Changes in culture expanded human amniotic epithelial cells: implications for potential therapeutic applications
    Gita Pratama
    Centre for Reproduction and Development, Monash Institute of Medical Research, Monash University, Clayton, Victoria, Australia
    PLoS ONE 6:e26136. 2011
    ..These findings suggest that primary and expanded hAEC may be suitable for different cellular therapeutic applications...
  38. ncbi Amniotic membrane application reduces liver fibrosis in a bile duct ligation rat model
    Luciana B Sant'anna
    Centro di Ricerca E Menni, Fondazione Poliambulanza Istituto Ospedaliero, Via Bissolati 57, Brescia, Italy
    Cell Transplant 20:441-53. 2011
    ..These findings suggest that human AM, when applied as a patch onto the liver surface, might inhibit fibrosis progression in BDL-injured livers, and could protect against hepatic damage associated with fibrotic degeneration...
  39. pmc Surfactant protein-A as an anti-inflammatory component in the amnion: implications for human pregnancy
    Deug Chan Lee
    Perinatology Research Branch, Department of Health and Human Services, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, USA
    J Immunol 184:6479-91. 2010
    ..a potential role for SP-A in human pregnancy and parturition by examining SP-A expression patterns in AF and amnion. High molecular mass (>250 kDa) oligomeric SP-A was increased in AF with advancing gestation...
  40. ncbi The amniotic membrane in ophthalmology
    Harminder S Dua
    Division of Ophthalmology and Visual Sciences, University of Nottingham, Nottingham, UK
    Surv Ophthalmol 49:51-77. 2004
    ..Further studies will undoubtedly reveal the true potential of the membrane, its mechanism(s) of action, and the effective use of this tissue in ophthalmology...
  41. ncbi Labor and inflammation increase the expression of oxytocin receptor in human amnion
    Vasso Terzidou
    Imperial College London, Parturition Research Group, Institute of Reproductive and Developmental Biology, Hammersmith Hospital Campus, Du Cane Road, London, United Kingdom
    Biol Reprod 84:546-52. 2011
    The oxytocin/oxytocin receptor (OXT/OXTR) system plays an important role in the regulation of parturition. The amnion is a major source of prostaglandins and inflammatory cytokine synthesis, which increase both before and during labor...
  42. ncbi Identification of stem cell marker-positive cells by immunofluorescence in term human amnion
    Toshio Miki
    Department of Pathology, University of Pittsburgh, Pittsburgh, PA 15213, USA
    J Reprod Immunol 75:91-6. 2007
    ..Stem cell marker-positive cells were found in amnion but not in choriodecidua, tissues known to contain hematopoietic and trophoblastic stem cells...
  43. pmc Epidermal growth factor and interleukin-1beta utilize divergent signaling pathways to synergistically upregulate cyclooxygenase-2 gene expression in human amnion-derived WISH cells
    William E Ackerman
    Department of Obstetrics and Gynecology Division of Maternal Fetal Medicine and Laboratory of Perinatal Research, The Ohio State University, College of Medicine and Public Health, Columbus, Ohio 43210, USA
    Biol Reprod 71:2079-86. 2004
    ..It was previously demonstrated that EGF could potentiate IL-1beta-driven PGE(2) production in amnion and amnion-derived (WISH) cells...
  44. ncbi Factors secreted by human amniotic epithelial cells promote the survival of rat retinal ganglion cells
    Saiko Uchida
    Department of Ophthalmology, Tokyo University School of Medicine, Tokyo 113 8655, Japan
    Neurosci Lett 341:1-4. 2003
    ..These results suggest that HAECs may produce unknown neuroprotective factors, suggesting its potential for the treatment of RGC degenerative diseases...
  45. ncbi Xenografted human amniotic membrane-derived mesenchymal stem cells are immunologically tolerated and transdifferentiated into cardiomyocytes
    Hiroko Tsuji
    Department of Obstetrics, Keio University School of Medicine, Tokyo, Japan
    Circ Res 106:1613-23. 2010
    ..Amniotic membrane is known to have the ability to transdifferentiate into multiple organs and is expected to stimulate a reduced immunologic reaction...
  46. ncbi Carbodiimide cross-linked amniotic membranes for cultivation of limbal epithelial cells
    David Hui Kang Ma
    Limbal Stem Cell Laboratory, Department of Ophthalmology, Chang Gung Memorial Hospital, Taoyuan 33305, Taiwan, ROC
    Biomaterials 31:6647-58. 2010
    ..It is concluded that the AM cross-linked with 0.05 mmol EDC/mg AM may be a potential biomaterial for regenerative medicine...
  47. ncbi Functional screening of TLRs in human amniotic epithelial cells
    Claire Gillaux
    INSERM, U1016, département génétique et développement, Institut Cochin, Centre National de la Recherche Scientifique, Unité Mixte de Recherche 8104, Universite Paris Descartes, Paris, France
    J Immunol 187:2766-74. 2011
    ..These data suggest specific TLR-mediated functions in human amniotic epithelial cells for initiating different immune responses, which ultimately may lead to preterm birth...
  48. ncbi Amniotic membrane and amniotic fluid-derived cells: potential tools for regenerative medicine?
    Ornella Parolini
    Centro di Ricerca E Menni, Fondazione Poliambulanza Istituto Ospedaliero, Via Bissolati, 57, 25124 Brescia, Italy
    Regen Med 4:275-91. 2009
    ....
  49. ncbi Human amnion epithelial cells prevent bleomycin-induced lung injury and preserve lung function
    Sean Murphy
    The Ritchie Centre, Monash Institute of Medical Research, Monash University, Clayton, Australia
    Cell Transplant 20:909-23. 2011
    Human amnion epithelial cells (hAECs) have attracted recent attention as a promising source of cells for regenerative therapies, with reports that cells derived from human term amnion possess multipotent differentiation ability, low ..
  50. ncbi Isolation and characterization of Oct-4+/HLA-G+ mesenchymal stem cells from human umbilical cord matrix: differentiation potential and detection of new markers
    Giampiero La Rocca
    Sezione di Anatomia Umana, Dipartimento di Medicina Sperimentale, Universita degli Studi di Palermo, Via del Vespro 129, 90127, Palermo, Italy
    Histochem Cell Biol 131:267-82. 2009
    ....
  51. ncbi The potential of amniotic membrane/amnion-derived cells for regeneration of various tissues
    Ayaka Toda
    Department of Regenerative Medicine, University of Toyama Graduate School of Medicine and Pharmaceutical Sciences, 2630 Sugitani, Toyama 930 0194 Japan
    J Pharmacol Sci 105:215-28. 2007
    ..It is of interest as a potential alternative to complicated tissue/organ transplantation. Recently, amnion-derived cells have been reported to have multipotent differentiation ability, and these cells have attracted ..
  52. ncbi Differentiation of mesenchymal cells derived from human amniotic membranes into hepatocyte-like cells in vitro
    Tomoharu Tamagawa
    Institute of Cell Biology, Ishiwata Hospital, RIKEN BioResource Centre, Ibaraki, Japan
    Hum Cell 20:77-84. 2007
    ..Although further studies should be carried out to determine whether such in vitro-differentiated cells can function in vivo as hepatocytes. These cells may be useful in various applications that require human hepatocytes...
  53. ncbi Human amniotic mesenchymal cells have some characteristics of cardiomyocytes
    Peng Zhao
    Department of Organ Regeneration, Institute of Organ Transplants, Reconstructive Medicine and Tissue Engineering, Shinshu University School of Medicine, Matsumoto, Japan
    Transplantation 79:528-35. 2005
    ..Here, we report that human amniotic mesenchymal cells (hAMC), which are multipotent cells derived from fetal mesoderm, may be a suitable cell source for CCM...
  54. ncbi Human amniotic epithelial cells ameliorate behavioral dysfunction and reduce infarct size in the rat middle cerebral artery occlusion model
    Tianjin Liu
    Genetic Engineering Group, Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai, China
    Shock 29:603-11. 2008
    ..This model holds tremendous potential for development into wide use in cell-mediated gene therapy in the future...
  55. ncbi Transplantation of human amnion epithelial cells reduces hepatic fibrosis in immunocompetent CCl₄-treated mice
    Ursula Manuelpillai
    Centre for Reproduction and Development, Monash Institute of Medical Research, Monash University, Clayton, Victoria, Australia
    Cell Transplant 19:1157-68. 2010
    ..To test this hypothesis we transplanted human amnion epithelial cells (hAEC), isolated from term delivered placenta, into immunocompetent C57/BL6 mice at week 2 of a 4-..
  56. ncbi Efficient reprogramming of human and mouse primary extra-embryonic cells to pluripotent stem cells
    Shogo Nagata
    Stem Cell Engineering, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara cho, Shogoin, Sakyo ku, Kyoto 606 8507, Japan
    Genes Cells 14:1395-404. 2009
    ..Here, we demonstrate that newborn human (h) and mouse (m) extra-embryonic amnion (AM) and yolk-sac (YS) cells, in which endogenous KLF4/Klf4, c-MYC/c-Myc and RONIN/Ronin are expressed, can be ..
  57. ncbi Properties of the amniotic membrane for potential use in tissue engineering
    Hassan Niknejad
    Department of Nanomedicine and Tissue Engineering, Shaheed Beheshti Medical University, Tehran, Iran
    Eur Cell Mater 15:88-99. 2008
    ..In this review, the various properties of the AM are discussed in light of their potential use for TE...
  58. ncbi Amnionless, essential for mouse gastrulation, is mutated in recessive hereditary megaloblastic anemia
    Stephan M Tanner
    Human Cancer Genetics Program, Comprehensive Cancer Center, The Ohio State University, Columbus, Ohio 43210, USA
    Nat Genet 33:426-9. 2003
    ..Mice homozygous with respect to the amn mutation generated by a transgene insertion have no amnion. The embryos are severely compromised, surviving to the tenth day of gestation but seem to lack the mesodermal ..
  59. ncbi Identification of matrix metalloproteinase-9 in amniotic fluid and amniochorion in spontaneous labor and after experimental intrauterine infection or interleukin-1 beta infusion in pregnant rhesus monkeys
    Felipe Vadillo-Ortega
    Department of Research Direction and Electron Microscopy, Instituto Nacional de Perinatologia, Mexico City, Mexico
    Am J Obstet Gynecol 186:128-38. 2002
    ..The purpose of this study was to examine the roles of intrauterine infection, inflammation, and spontaneous labor on the expression of matrix metalloproteinase-9 in fetal membranes and amniotic fluid of late pregnant rhesus monkeys...
  60. ncbi Immunogenicity of human amniotic membrane in experimental xenotransplantation
    M Kubo
    Department of Ophthalmology, Tokyo Medical University, Tokyo, Japan
    Invest Ophthalmol Vis Sci 42:1539-46. 2001
    ..The immunogenic characterization of amniotic membrane is still unknown. This study was designed to examine the immunogenicity of human amniotic membrane, by using experimental xenotransplantation models...
  61. pmc Human spontaneous labor without histologic chorioamnionitis is characterized by an acute inflammation gene expression signature
    Ramsi Haddad
    The Perinatology Research Branch, National Institute of Child Health and Human Development, NIH, DHHS, Bethesda, MD, USA
    Am J Obstet Gynecol 195:394.e1-24. 2006
    ..The purpose of this study was to identify which biological processes may be involved in normal labor...
  62. ncbi A single nucleotide polymorphism in the matrix metalloproteinase-1 (MMP-1) promoter influences amnion cell MMP-1 expression and risk for preterm premature rupture of the fetal membranes
    Toshio Fujimoto
    Center for Research on Reproduction and Women s Health, University of Pennsylvania Medical Center, Philadelphia, Pennsylvania 19104, USA
    J Biol Chem 277:6296-302. 2002
    ..We investigated whether this polymorphism is functionally significant for MMP-1 expression in amnion cells and whether it is associated with preterm premature rupture of the membranes (PPROM)...
  63. ncbi Amniotic membrane use in ophthalmology
    Jose A P Gomes
    Advanced Ocular Surface Center, Brazil
    Curr Opin Ophthalmol 16:233-40. 2005
    ..The purpose of this review is to describe the most recent and relevant clinical and experimental data about the use of amniotic membrane in ophthalmology...
  64. pmc Suppression of interleukin 1alpha and interleukin 1beta in human limbal epithelial cells cultured on the amniotic membrane stromal matrix
    A Solomon
    Ocular Surface and Tear Center, Bascom Palmer Eye Institute, Department of Ophthalmology, University of Miami School of Medicine, Miami, Florida, USA
    Br J Ophthalmol 85:444-9. 2001
    ..These data may explain in part the effect of AM transplantation in reducing ocular surface inflammation, underscoring the unique feature of the AM as a substrate for tissue engineering...
  65. ncbi The variation in transparency of amniotic membrane used in ocular surface regeneration
    C J Connon
    School of Pharmacy, University of Reading, RM 108, Hopkins Building Whiteknights, PO Box 228, Reading RG6 6UB, UK
    Br J Ophthalmol 94:1057-61. 2010
    ..Therefore, we applied our experience of quantifying corneal transparency to AM...
  66. pmc Human amniotic epithelial cells as novel feeder layers for promoting ex vivo expansion of limbal epithelial progenitor cells
    Ying Ting Chen
    TissueTech Inc, and Ocular Surface Center, Miami, FL 33173, USA
    Stem Cells 25:1995-2005. 2007
    ..Disclosure of potential conflicts of interest is found at the end of this article...
  67. ncbi Human amniotic membrane transplantation: past, present, and future
    Thomas John
    Loyola University at Chicago, Department of Ophthalmology, 2160 South First Avenue, Maywood, IL 60153, USA
    Ophthalmol Clin North Am 16:43-65, vi. 2003
    ..The procurement, complications, and solutions of this delicate procedure are covered with a mention of what is in store for the future...
  68. ncbi Antimicrobial peptides in amniotic fluid: defensins, calprotectin and bacterial/permeability-increasing protein in patients with microbial invasion of the amniotic cavity, intra-amniotic inflammation, preterm labor and premature rupture of membranes
    J Espinoza
    Perinatology Research Branch, National Institute of Child Health and Human Development NIH DHSS, Bethesda, Maryland, USA
    J Matern Fetal Neonatal Med 13:2-21. 2003
    ....
  69. ncbi Improving electrophysiologic and histologic outcomes by photochemically sealing amnion to the peripheral nerve repair site
    Francis P Henry
    Plastic Surgery Research Laboratory, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA
    Surgery 145:313-21. 2009
    ..This study determines whether isolation of the nerve repair site using photochemical tissue bonding (PTB) in combination with human amniotic membrane can improve both functional and histologic recovery...
  70. ncbi Investigating the efficacy of amnion-derived compared with bone marrow-derived mesenchymal stromal cells in equine tendon and ligament injuries
    Anna Lange-Consiglio
    Universita degli Studi di Milano, Large Animal Hospital, Reproduction Unit, Lodi, Italy
    Cytotherapy 15:1011-20. 2013
    ..The objective was to prove the ability of AMSCs to exert beneficial effects in vivo...
  71. ncbi Human amnion epithelial cells as a treatment for inflammation-induced fetal lung injury in sheep
    Patricia Vosdoganes
    The Ritchie Centre, Monash Institute of Medical Research, Department of Obstetrics and Gynaecology, Monash University, Clayton, Vic, Australia
    Am J Obstet Gynecol 205:156.e26-33. 2011
    The purpose of this study was to determine whether human amnion epithelial cells (hAECs) can modulate the pulmonary developmental consequences of intrauterine inflammation in fetal sheep that are exposed to intraamniotic ..
  72. pmc Technique of amniotic membrane transplant dressing in the management of acute Stevens-Johnson syndrome
    Mahiul M K Muqit
    Cornea and Anterior Segment Service, York Hospital NHS Trust, Wigginton Road, York YO31 8HE, UK
    Br J Ophthalmol 91:1536. 2007
    ..To report the technique of amniotic membrane transplantation dressing during the acute phase of Stevens-Johnson syndrome...
  73. ncbi Interleukin (IL)-6 and IL-8 production by human amnion: regulation by cytokines, growth factors, glucocorticoids, phorbol esters, and bacterial lipopolysaccharide
    J A Keelan
    Department of Pharmacology and Clinical Pharmacology, School of Medicine, University of Auckland, New Zealand
    Biol Reprod 57:1438-44. 1997
    ..The source of these cytokines has not been identified, although the fetal membranes (amnion and chorion) are likely contributors...
  74. ncbi Phenotypic and functional heterogeneity of human bone marrow- and amnion-derived MSC subsets
    Kavitha Sivasubramaniyan
    Department of Internal Medicine II, Division of Hematology, Immunology, Oncology, Rheumatology and Pulmonology, University Clinic of Tubingen, Tubingen, Germany
    Ann N Y Acad Sci 1266:94-106. 2012
    ..In this report, we summarize the most frequently used markers and introduce new targets for antibody-based isolation procedures of primary bone marrow- and amnion-derived MSCs.
  75. ncbi Amniotic membrane grafting for conjunctival and lid surface disease in the acute phase of toxic epidermal necrolysis
    Anamika Tandon
    Princess Alexandra Eye Pavilion, Edinburgh, Scotland, UK
    J AAPOS 11:612-3. 2007
    ..We present a case of TEN with severe bilateral ocular involvement in whom we successfully used amniotic membrane grafts to treat the ocular surface during the acute phase of the disease...
  76. ncbi In vitro secretion profile of pro-inflammatory cytokines IL-1β, TNF-α, IL-6, and of human beta-defensins (HBD)-1, HBD-2, and HBD-3 from human chorioamniotic membranes after selective stimulation with Gardnerella vaginalis
    Veronica Zaga-Clavellina
    Cell Biology Department, Instituto Nacional de Perinatologia Isidro Espinosa de los Reyes, Mexico City, Mexico
    Am J Reprod Immunol 67:34-43. 2012
    ..Preterm labor associated with infection is a major clinical condition; in this work, we analyze the response of human chorioamniotic membranes stimulated with Gardnerella vaginalis...
  77. pmc Isolation and biological characterization of chicken amnion epithelial cells
    Y Gao
    Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China
    Eur J Histochem 56:e33. 2012
    ..These results suggested that AECs isolated from chicken embryos exhibited the characteristics of the multipotent stem cells. AECs may therefore be ideal candidates for cellular transplantation therapy and tissue engineering...
  78. ncbi Cross-linking of human amniotic membrane by glutaraldehyde
    Eberhard Spoerl
    Department of Ophthalmology, Universitatsklinikum CGC, Dresden, Germany
    Ophthalmic Res 36:71-7. 2004
    ..However, in some diseases the transplant usually dissolves rather quickly and early detachment may occur. Therefore, we tried to stabilize the amniotic transplant by applying glutaraldehyde for collagen cross-linking of the membrane...
  79. ncbi Transplantation of human limbal epithelium cultivated on amniotic membrane for the treatment of severe ocular surface disorders
    Jun Shimazaki
    Department of Ophthalmology, Tokyo Dental College, 5 11 13 Sugano, Ichikawa shi, Chiba 272 8513, Japan
    Ophthalmology 109:1285-90. 2002
    ..To study the short-term clinical results of transplanting of cultivated corneal/limbal epithelial cells on human amniotic membrane (AM) for limbal deficiency...
  80. ncbi Amniotic membrane transplantation in acute phase of toxic epidermal necrolysis with severe corneal involvement
    Akira Kobayashi
    Department of Ophthalmology, Kanazawa University Graduate School of Medical Science, Kanazawa, Ishikawa, Japan
    Ophthalmology 113:126-32. 2006
    ..To report successful management of acute stage toxic epidermal necrolysis (TEN) by amniotic membrane transplantation...
  81. ncbi Premature rupture of the fetal membranes: is the amnion the major determinant?
    Michelle L Oyen
    Department of Biophysical Sciences and Medical Physics, University of Minnesota, Minneapolis, USA
    Am J Obstet Gynecol 195:510-5. 2006
    The objective of the study was to evaluate the relative contributions of amnion and chorion to the strength of fetal membranes and to correlate these findings with gestational age.
  82. ncbi Living skin equivalents constructed using human amnions as a matrix
    Lujun Yang
    Department of Dermatology, Ehime University Graduate School of Medicine, Toon City, Ehime, Japan
    J Dermatol Sci 56:188-95. 2009
    ..The amnionic membrane (AM) is known to have useful properties as a dermal matrix and can be used to construct a LSE...
  83. ncbi Epithelial reorganization events during late extraembryonic development in a hemimetabolous insect
    Kristen A Panfilio
    Institute for Developmental Biology, University of Cologne, Gyrhofstrabetae 17, 50931 Cologne, Germany
    Dev Biol 340:100-15. 2010
    As extra-embryonic tissues, the amnion and serosa are not considered to contribute materially to the insect embryo, yet they must execute an array of morphogenetic movements before they are dispensable...
  84. ncbi Induction of Galphas contributes to the paradoxical stimulation of cytosolic phospholipase A2alpha expression by cortisol in human amnion fibroblasts
    Chunming Guo
    School of Life Sciences, Fudan University, 220 Handan Road, Shanghai 200433, People s Republic of China
    Mol Endocrinol 24:1052-61. 2010
    ..down-regulation of cPLA(2alpha), up-regulation of cPLA(2alpha) by glucocorticoids has been reported in human amnion fibroblasts, which may play a key role in parturition...
  85. pmc Isolation and characterization of canine amniotic membrane-derived multipotent stem cells
    Sang Bum Park
    Adult Stem Cell Research Center, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea
    PLoS ONE 7:e44693. 2012
    ..Taken together, our results show that cAM-MSCs could be a rich source of stem cells in dogs. Furthermore, cAM-MSCs may be useful as a cell therapy application for veterinary regenerative medicine...
  86. ncbi Proteoglycans and hyaluronan in human fetal membranes
    M Meinert
    Research Laboratory, Department of Obstetrics and Gynecology, Aarhus University Hospital, Denmark
    Am J Obstet Gynecol 184:679-85. 2001
    ..RESULTS: The fetal membranes contained predominantly smaller proteoglycans, such as biglycan and decorin. The amnion consisted of typical fibrous connective tissue with a high concentration of collagen...
  87. ncbi Interleukin-8 release from human gestational tissue explants: the effects of lipopolysaccharide and cytokines
    N Laham
    Perinatal Research Centre, Department of Perinatal Medicine, The Royal Women s Hospital, Carlton, Victoria, Australia
    Biol Reprod 57:616-20. 1997
    ..In this study, we tested the hypotheses that IL-8 is released locally in the intrauterine environment from human amnion, choriodecidua, and placenta, and that IL-8 release from these tissues is increased by bacterial endotoxin, ..
  88. ncbi Human amnion mesenchyme cells express phenotypes of neuroglial progenitor cells
    Norio Sakuragawa
    Department of Regenerative Medicine, Toho University School of Medicine, Sagamihara, Kanagawa, Japan
    J Neurosci Res 78:208-14. 2004
    Previous studies from our laboratory showed that human amnion epithelial cells (AECs) have multiple functions, such as synthesis and release of catecholamines, acetylcholine, neurotrophic factors, activin, and noggin...
  89. pmc The role of the multiple banded antigen of Ureaplasma parvum in intra-amniotic infection: major virulence factor or decoy?
    Samantha J Dando
    Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Queensland, Australia
    PLoS ONE 7:e29856. 2012
    ..This host response may be important in the pathogenesis of inflammation-mediated adverse pregnancy outcomes...
  90. pmc The involvement of human amnion in histologic chorioamnionitis is an indicator that a fetal and an intra-amniotic inflammatory response is more likely and severe: clinical implications
    Chan Wook Park
    Department of Obstetrics and Gynecology, Seoul National University College of Medicine, 28 Yongon Dong, Chongno Gu, Seoul 110 744, Republic of Korea
    Placenta 30:56-61. 2009
    Amnionitis (inflammation of the amnion) is the final stage of extra-placental chorioamniotic inflammation...
  91. ncbi Micronucleus formation in human amnion cells after exposure to 50 Hz MF applied horizontally and vertically
    M Simko
    Institute of Animal Physiology, Division of Environmental Physiology, University of Rostock, Universitatsplatz 2, D 18055, Rostock, Germany
    Mutat Res 418:101-11. 1998
    ..Therefore, the reason for inconsistent results published in the literature may be caused by the variability of exposure systems, the exposure conditions and the cell types used...
  92. ncbi Protective effect of Astragali radix extract on interleukin 1beta-induced in fl ammation in human amnion
    Yun Hee Shon
    Department of Pharmacology, College of Medicine and Intractable Disease Research Center, Dongguk University, Kyongju 780 714, Korea
    Phytother Res 17:1016-20. 2003
    ..production and prostaglandin E(2) (PGE(2)) and leukotriene C(4) (LTC(4)) release from IL-1beta-stimulated human amnion. Primary monolayer cultures of amnion cells were established from women undergoing elective caesarean section ..
  93. pmc Nuclear factor kappa B activation occurs in the amnion prior to labour onset and modulates the expression of numerous labour associated genes
    Sheri Lim
    Imperial College Parturition Research Group, Institute of Reproduction and Developmental Biology, Imperial College London, London, United Kingdom
    PLoS ONE 7:e34707. 2012
    ..is an increase in the synthesis of prostaglandins, cytokines and chemokines in the fetal membranes, particular the amnion. This is associated with activation of the transcription factor nuclear factor kappa B (NFκB)...
  94. ncbi Tocilizumab inhibits interleukin-6-mediated matrix metalloproteinase-2 and -9 secretions from human amnion cells in preterm premature rupture of membranes
    Yukio Mano
    Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine, Showa Ku, Nagoya, Japan
    Gynecol Obstet Invest 68:145-53. 2009
    ....
  95. pmc A serum- and feeder-free technique of culturing human corneal epithelial stem cells on amniotic membrane
    Kaevalin Lekhanont
    Department of Ophthalmology, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand
    Mol Vis 15:1294-302. 2009
    ..To describe a simple technique of cultivating human corneal epithelial stem cells using an Epilife culture medium under serum- and feeder-free conditions...
  96. ncbi Ocular surface changes in limbal stem cell deficiency caused by chemical injury: a histologic study of excised pannus from recipients of cultured corneal epithelium
    A Fatima
    Stem Cell Biology Laboratory, LV Prasad Eye Institute, Hyderabad, India
    Eye (Lond) 22:1161-7. 2008
    ..To report histopathologic changes of the ocular surface pannus in patients with severe limbal stem cell deficiency (LSCD)...
  97. pmc Amniotic mesenchymal stem cells: a new source for hepatocyte-like cells and induction of CFTR expression by coculture with cystic fibrosis airway epithelial cells
    Valentina Paracchini
    Medical Genetics Laboratory, Fondazione IRCCS Ca Granda Ospedale Maggiore Policlinico, Via Commenda 12, 20122 Milan, Italy
    J Biomed Biotechnol 2012:575471. 2012
    ..Our data show that amniotic MSCs have the potential to differentiate into epithelial cells of organs relevant in CF pathogenesis and may contribute to partial correction of the CF phenotype...
  98. ncbi Amniotic membrane transplantation with conjunctival autograft for recurrent pterygium
    Jun Shimazaki
    Department of Ophthalmology, Tokyo Dental College, Chiba, Japan
    Ophthalmology 110:119-24. 2003
    ..To study the effect of amniotic membrane transplantation (AMT) combined with either limbal autograft transplantation (LAT) or conjunctival autograft transplantation (CAT) in recurrent pterygium...
  99. ncbi Amnion-derived pluripotent/multipotent stem cells
    Toshio Miki
    Department of Pathology, University of Pittsburgh, 200 Lothrop, St Pittsburgh, PA 15213, USA
    Stem Cell Rev 2:133-42. 2006
    ..In this review, we describe the unique characteristics of the AE stem cells and summarize previous work concerning the stem cell nature of cells from amnion.
  100. ncbi Experimental study on allografts of amniotic epithelial cells in calcaneal tendon lesions of sheep
    A Muttini
    Department of Veterinary Clinical Sciences, University of Teramo, Teramo, Italy
    Vet Res Commun 34:S117-20. 2010
    ..In addition, after 1 month, the neo-collagen began to be organized into parallel arrays of fibers oriented along the longitudinal axis of the tendon...
  101. ncbi Isolation and characterization of mesenchymal stem cells from the amniotic membrane
    Carmen Mihaela Mihu
    Department of Histology, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj Napoca, Romania
    Rom J Morphol Embryol 50:73-7. 2009
    ..HLA expression provides information that might help explain the immunological mechanisms of tolerance between the maternal organism and fetal structures...

Research Grants63

  1. MECHANISMS OF FETAL MEMBRANE RUPTURE
    Jerome F Strauss; Fiscal Year: 2010
    ..promoter activity mitigating the effect of the [unreadable]656 T allele;4) collagen synthesis is reduced in amnion fibroblasts carrying the [unreadable]656 T allele, but not the [unreadable]656 T allele in the presence of the 12 ..
  2. Cellular Mechanisms of Amniotic Fluid Volume Regulation
    Cecilia Y Cheung; Fiscal Year: 2013
    ..These studies will be carried out in ovine amnion cells in vitro and chronically catheterized ovine fetuses in vivo...
  3. Allogeneic Placental Stem Cells for Tissue Repair and Regeneration
    Vijay Kumar; Fiscal Year: 2010
    Discrete populations of stem cells reside within placental tissue (amnion, chorion, and umbilical cord matrix)...
  4. Human Amnion Cell Model for Investigation of Amniotic Fluid Volume
    Cecilia Y Cheung; Fiscal Year: 2012
    ..in experimental animals have generated most of the current knowledge on AF transport characteristics across the amnion, the rate-limiting layer for transport...
  5. Repair of iatrogenic fetal membrane defects with an adhesive tissue scaffold
    Russell J Stewart; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Healthy and intact fetal membranes (amnion and chorion) are required for optimal pregnancy outcomes...
  6. REGULATION OF VEGF EXPRESSION IN PLACENTA AND MEMBRANES
    CECILIA CHEUNG; Fiscal Year: 2004
    ..Recently, we found expression of vascular endothelial growth factor (VEGF) in the amnion and chorion, and this expression is increased under conditions of enhanced intramembranous absorption...
  7. Fetal Membrane AQP Expression/Amniotic Fluid Regulation
    Michael Ross; Fiscal Year: 2004
    ..In situ hybridization reveals that AQP8 is expressed in epithelial cells of human amnion and chorion, as well as trophoblasts of placenta...
  8. SIGNAL TRANSDUCTION MECHANISMS AND AMNION PGE2 SYNTHESIS
    Leslie Myatt; Fiscal Year: 1993
    Synthesis and release of eicosanoids by amnion in response to a fetal or maternal signal may be involved the onset of parturition in humans but the intracellular signal transduction mechanisms that lead to eicosanoid synthesis (the ..
  9. INHIBITION OF UTERINE CONTRACTIONS BY FETAL MEMBRANES
    Patricia Collins; Fiscal Year: 1999
    ..and that the inhibitory effect is specific to the chorion/decidual side because membranes mounted with the amnion side toward the muscle did not show inhibition...
  10. The Molecular Pathogenesis of Health Disparities in Inf*
    Emmet Hirsch; Fiscal Year: 2005
    ..In the second phase of the project, we analyze human tissues (myometrium, placenta, chorion, amnion, decidua, amniocytes and blood) collected from approximately 1360 patients presenting in term and preterm labor in ..
  11. REGULATION OF PHOSPHOLIPASE A2 IN HUMAN AMNION
    Leslie Myatt; Fiscal Year: 2009
    The amnion is a major site of PGE2 production during labor and is a rich source of arachidonic acid, the precursor of PG production...
  12. In-ovo vaccination/avian influenza/bird/human
    DE CHU TANG; Fiscal Year: 2006
    ..Both vaccines will be injected into the amnion of chicken embryos...
  13. GLUCOCORTICOIDS AND MATRIX PROTEINS AT PARTURITION
    Seth Guller; Fiscal Year: 1999
    ..completely suppress the synthesis of FN and other ECM proteins in primary cultures of human cytotrophoblasts and amnion epithelial cells...
  14. ENDOTHELIAL CELL COMMUNICATION IN HOMEOSTASIS
    David Shepro; Fiscal Year: 1990
    ..treatment with barrier-mediating agents, cultures will be grown on microcarrier beads or on human acellular amnion membranes. These cultures provide a two-compartment assay to measure cell and macromolecule transit...
  15. INTERLEUKIN-1 IN HUMAN PREGNANCY
    Roberto Romero; Fiscal Year: 1991
    ..the ontogeny of fetal monocyte IL-1 production; 6) determine the effect of purified human IL-1 and bacterial products in amnion, chorion and decidual production of PGs; and 7) determine whether IL-1 can induce labor in an animal model.
  16. Fetal membrane fibroblasts:Proliferation/collagen synth
    MARK PLESSINGER; Fiscal Year: 2004
    ..rates, mitogenic responses, and effects upon collagen synthesis in recently isolated fibroblasts from the amnion and from the chorion in response to growth and immune factors endogenous to the fetal membranes...
  17. DOPAMINERGIC NEUROTROPHIC FACTORS AND TOURETTE SYNDROME
    MEG PALMATIER; Fiscal Year: 1999
    ..The specific aims are; 1. Purify the protein for the dopaminergic neurotrophic factor, amnion- derived neurite-promoting factor (ANPF), and clone, sequence, and map the gene for ANPF. 2...
  18. BIOMOLECULAR BASIS OF INFECTION-INDUCED PRETERM LABOR
    Susan Cox; Fiscal Year: 1990
    ..evaluate the mechanisms that are operative in the expression of receptors for cytokines in decidua, chorion leave, amnion, and villous trophoblastic tissues...
  19. ROLE OF CATECHOLAMINES IN THE INITIATION OF LABOR
    CHARLES DUCSAY; Fiscal Year: 1990
    ..Specifically, what are the stimulatory effects of CATS on PG biosynthesis by decidua, myometrium, amnion, and chorion? PG (PGF2, PGE2 and 6-keto-PGF1alpha) production will be quantitated before and after exposure of the ..
  20. ENDOGENOUS REGULATION OF LEUKOTRIENE SYNTHESIS
    STEVEN FEINMARK; Fiscal Year: 1990
    ..migration studies and electrical resistance measurements will be performed and will use EC monolayers grown on amnion. PMNL intracellular calcium measurements will be done in collaboration with Dr...
  21. REGULATION OF HUMAN DECIDUAL PROLACTIN
    Rebecca Jackson; Fiscal Year: 1992
    ..modulates a wide diversity of biologic actions including fetal lung maturation and transport of water across the amnion. Studies of the regulation of the human prolactin gene have been limited in part by the relative lack of ..
  22. LEUKOTRIENES AND LEUKOCYTE/VASCULAR CELL INTERACTIONS
    STEVEN FEINMARK; Fiscal Year: 1993
    ..Samuel Silverstein and will use EC monolayers grown on amnion. These novel interactions which provide a source of substrate and biochemical modulation of local vascular LTC4 ..
  23. Differentiation of Amnion-Derived Cells to Islet Cell Types
    Diana Clarke; Fiscal Year: 2006
    ..Stemnion's objective is to use human amnion epithelial stem cells to generate a population of glucose-responsive islet-like cells...
  24. CULTIVATION OF VIRUSES IN INFLAMMATORY BOWEL DISEASE
    Gary Gitnick; Fiscal Year: 1980
    ..colon cultures, human diploid lung cells, primary monkey kidney cells, human embryonic kidney and primary human amnion cultures as well as Vero monkey kidney cultures and human fetal intestinal cultures...
  25. ROLE OF PROTO-ONCOGENES IN MAMMALIAN EMBRYOGENESIS
    THOMAS SADLER; Fiscal Year: 2000
    ..a. Antisense oligonucleotides targeted to each of the genes will be microinjected into the amnion of 3-5 somite stage mouse embryos undergoing the processes of neurulation and gastrulation...
  26. CHEMICAL PROGRESSION AND INHIBITION OF NEOPLASIA
    Gene Siegal; Fiscal Year: 1991
    ..have recently shown that in a system of clonally derived cells, the invasive ability, as judged by this in vitro amnion model system, correlates and cosegregates with tumorigenicity...
  27. Lipid Bodies and Prostaglandin Production in Labor
    William E Ackerman; Fiscal Year: 2010
    ..the role of lipid bodies (LBs) in arachidonic acid (AA) storage, mobilization, and metabolism within cells of the amnion epithelium...
  28. INTERACTION OF ENDOTHELIAL CELLS AND LEUKOCYTES
    Samuel Silverstein; Fiscal Year: 1993
    ..difficulties we have developed a system for maintaining human ECs in monolayer cultures on a substrate of human amnion. ECs maintained in this way form confluent monolayers...
  29. OXYTOCIN RECEPTOR MEDIATED PROSTAGLANDIN RELEASE
    MELVYN SOLOFF; Fiscal Year: 2000
    DESCRIPTION: (Adapted from the applicant's abstract) Work described in this application will involve the use of amnion cells to elucidate principal mechanisms of up and down regulation of the OTR...
  30. MECHANISM OF HUMAN TRANSENDOTHELIAL LEUKOCYTE MIGRATION
    Samuel Silverstein; Fiscal Year: 1992
    ..permeability across an endothelial cells (EC) barrier, I have cultured human EC monolayers on a substrate of human amnion. I have measured the permeability of these monolayers and changes in EC cytosolic free calcium ((Ca++)i) during ..
  31. FOS ONCOGENE IN DEVELOPMENT
    Eileen Adamson; Fiscal Year: 1993
    ..Extraembryonic tissues express the highest levels (placenta, amnion, visceral yolk sac, etc...
  32. FETAL ISOENZYME EXPRESSION IN HUMAN TUMOR XENOGRAFTS
    Robert Singer; Fiscal Year: 1980
    ..A new onco-amnion (FL) isoenzyme is expressed in both of these cell lines...
  33. ISOLATION OF PEMPHIGUS ANTIGEN CDNA
    Madeleine Duvic; Fiscal Year: 1990
    ..We propose to screen a human cDNA expression library made from human amnion and placenta messenger RNA using purified labeled pemphigus antibody as a probe...
  34. AMNION APOPTOSIS IS MEDIATED VIA PROSTAGLANDINS
    John Moore; Fiscal Year: 2003
    DESCRIPTION: (Adapted from the Investigator's Abstract): It is proposed that amnion apoptosis requires: (1) Activation of cyclooxygenase-2; (2) Funneling of PGDH2, the cyclooxygenase product, to PGD2, and thereafter to metabolites of ..
  35. FELINE/FIV MODEL FOR PEDIATRIC AIDS
    Wayne Tompkins; Fiscal Year: 1993
    ..To study mode of transmission, placental tissues, amnion, cord blood and mammary tissues will be evaluated for FIV and Ab to FIV...
  36. MECHANISMS OF PRETERM LABOR: NF-KB ACTIVATION OF COX-2
    DOUGLAS KNISS; Fiscal Year: 2001
    ..Using amnion-derived WISH cells as a model system, they will examine whether NF-kB is activated following cytokine challenge...
  37. Mechanisms of Vascular Injury in the preterm lung
    Suhas Kallapur; Fiscal Year: 2006
    ..The experimental design in this proposal incorporates challenging but, particularly in this environment achievable goals that will provide important knowledge in the pathogenesis of bronchopulmonary dysplasia. ..
  38. TRANSPLANTATION OF EXPANDED LIMBAL EPITHELIAL STEM CELLS
    Scheffer Tseng; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  39. DEVELOPMENT OF SUTURELESS AMNIOTIC MEMBRANE GRAFT
    Scheffer Tseng; Fiscal Year: 2007
    ..Taken together, we may further promote our business development by expanding the market potential using Prokera(tm) for suture-less AMT. [unreadable] [unreadable]..
  40. Compartmental analysis of proteomic biomarkers during intra-uterine infections
    PETA GRIGSBY; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  41. HYPOXIA EFFECTS ON AMNIOTIC FLUID VOLUME
    ROBERT BRACE; Fiscal Year: 2008
    ..pathway will be determined by studying the effects of VEGF on transport in isolated amniotic membrane and amnion cells in culture...
  42. ANTI-SCARRING AND ANTI-INFLAMMATORY EFFECTS OF AMNIOTIC MEMBRANE EXTRACTS
    Scheffer Tseng; Fiscal Year: 2006
    ..unreadable] [unreadable] [unreadable]..
  43. OXIDATIVE DAMAGE AND PREMATURE RUPTURE OF THE MEMBRANES
    James Woods; Fiscal Year: 2004
    ..laboratory demonstrate that, in physiologic concentrations, the ROS, hypochlorous acid and superoxide, do damage amnion epithelium and collagen I, the strongest component of chorioamnion...
  44. EX VIVO EXPANSION OF RPE CELLS FOR TRANSPLANTATION
    Scheffer Tseng; Fiscal Year: 2005
    ..Success in accomplishing these goals will allow us to engineer this new tissue-based "Biologies" to restore sights in patients suffering ARMD or other retinal diseases in which RPE is dysfunctional. ..
  45. EARLY EVENTS IN NEURAL AND HOMEOGENETIC INDUCTION
    Claudio Stern; Fiscal Year: 2005
    ..D. To explore the possible roles of 6 newly-identified candidate secreted factors in the early steps of neural induction and homeogenetic induction. ..
  46. MIDCAREER AWARD IN PATIENT ORIENTED OBSTETRIC RESEARCH
    Dwight Rouse; Fiscal Year: 2004
    ..Finally, the effectiveness of an intervention (how it would perform under conditions of actual clinical practice) is an attribute that, in many situations, is as important as its efficacy (how it performs under ideal conditions). ..
  47. OVARIAN STEROID REGULATION OF SEROTONIN IN PRIMATES
    CYNTHIA LOUISE BETHEA; Fiscal Year: 2010
    ..Altogether, we will show that ovarian steroids support serotonin neuron survival and we will provide pivotal underlying genomic mechanisms. ..
  48. Defining the Mammalian Intercellular Bridge Interactome
    Martin M Matzuk; Fiscal Year: 2010
    ..Our proposed Specific Aims will also help us to develop additional high throughput screening assays for the identification of small molecule disruptors of the intercellular bridge. ..
  49. MOUSE MODELS TO STUDY GONADAL TUMOR DEVELOPMENT
    Martin M Matzuk; Fiscal Year: 2010
    ..Characterization of these mice will lead to important insights into the development of gonadal cancers in men and women and the roles of several proteins in reproductive physiology. ..
  50. Mouse Model For Amniotic Fluid Volume Regulation
    CECILIA CHEUNG; Fiscal Year: 2002
    ..This pathway is constituted by a network of microvessels on the surface of the placenta and, in sheep, within the amnion and chorion as well...
  51. Immortalization of Pregnant Human Myometrial Cells
    MELVYN SOLOFF; Fiscal Year: 2004
    ....
  52. VASCULOGENESIS IN THE MURINE ALLANTOIS
    KAREN DOWNS; Fiscal Year: 2002
    ..Further, results of our experiments will shed light on the mechanism of formation of the endothelial cell lineage. ..
  53. DERMAL-EPIDERMAL INTERACTIONS IN DIABETIC WOUND HEALING
    Timothy Crombleholme; Fiscal Year: 2004
    ..abstract_text> ..
  54. Oral Infection in the Pathogenesis of Gestational Diabetes
    Lynnae Millar; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  55. Bridges to the Doctorate in Reproductive Biology
    Fuller Bazer; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  56. Endothelial Progenitor Cell Biology in Type 1 Diabetes
    Timothy Crombleholme; Fiscal Year: 2008
    ..3. To determine the mechanisms by which type I diabetes alters EPC gene expression and function. ..
  57. EXPERIMENTAL MODEL FOR CHORIOAMNIONITIS AND PREMATURITY
    Michael Gravett; Fiscal Year: 2005
    ..These studies will clarify the pathophysiology of infection-associated preterm labor and will suggest effective interventional strategies. ..
  58. CORE GRANT FOR VISION RESEARCH
    Herbert Kaufman; Fiscal Year: 2008
    ..This Core Grant has [unreadable] been a vital force in keeping vision research highly active at LSU for the past 25 years, and we hope that it will continue to serve this function in the future. [unreadable] [unreadable]..
  59. CHLORHEXIDINE IRRIGATION TO PREVENT INFECTION
    Dwight Rouse; Fiscal Year: 2001
    ....
  60. Twin-Twin Transfusion Syndrome Trial
    Timothy Crombleholme; Fiscal Year: 2004
    ..The overall goal of the study is to improve the outcomes of twins with TTTS by determining which treatment for TTTS has a better survival as well as cardiac, neurologic, and developmental outcome. ..
  61. OCULAR HERPES SIMPLEX
    Herbert Kaufman; Fiscal Year: 2008
    ..If they are additive to or synergistic with acyclovir in suppressing viral DNA production, tests on human infection could almost certainly follow. [unreadable] [unreadable] [unreadable]..