corneal endothelium

..cytokeratins (CKs) 8 and 18--typical epithelial cell markers--are constitutively expressed in adult human corneal endothelium. Cryosections, paraffin-embedded sections and corneal endothelial imprints obtained from eleven adult human ..

..However, these data on reassembly show that a contractile tone of the PAMR is essential for assembly of the apical junctional complex...

..To describe Descemet membrane endothelial keratoplasty (DMEK) with organ cultured Descemet membrane (DM) in a human cadaver eye model and a patient with Fuchs endothelial dystrophy...

The corneal endothelium maintains stromal deturgescence, which is a prerequisite for corneal transparency...

..extracellular matrix macromolecules found in Descemet's membrane and in retrocorneal fibrous membrane (RCFM), and to examine whether the corneal endothelium has the capacity to produce both basement and non-basement membrane phenotypes.

..In addition, these cells might be of value in culturing or as a source for the synthesis of artificial corneas...

..3 channel produces an A-current although expression of constructs which lack the 5' region of the first start codon inactivate much more slowly than full-length constructs. This short hKv3.3 construct also shows changes in activation...

..efficacy and pathogenicity of three commercially available nonviral DNA vectors for gene transfer to the corneal endothelium. METHODS: Corneas obtained from New Zealand White rabbits were cultured ex vivo...

To understand the role of TGF-beta related signals in the repair of a corneal endothelium defect and also to evaluate the therapeutic effect of Smad7 gene transfer on injury induced fibrosis of the corneal endothelium in rats.

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The purpose of this study was to characterize age-associated changes in the corneal endothelium and Descemet's membrane (DM) in C57BL/6 mice, an inbred strain commonly used as a genetic disease model...

PURPOSE: Chloride and bicarbonate are necessary for maintenance of fluid transport by the corneal endothelium, however there is little information on the identity of anion transport proteins that could serve as anion efflux mechanisms in ..

..To investigate the effect of an intracameral injection of bevacizumab on corneal endothelial cells in rabbits...

..To evaluate the endothelial cell count change in eyes implanted with the iris-claw phakic Artisan lens for treatment of moderate to high myopia...

..To determine the effect of intracameral injection of preservative-free lidocaine 1% and carbachol 0.01% on corneal endothelial cells of rabbits...

To determine the effects of proinflammatory cytokines on differential gene expression profiles in the human corneal endothelium (HCE), by using a cDNA expression array.

The corneal endothelium maintains corneal deturgescence and clarity by a pump-leak mechanism first described by David Maurice. This cell layer can be investigated clinically with specular microscopy, fluorophotometry, and pachymetry...

..The effects of exogenous HGF and KGF, compared to epidermal growth factor, on the proliferation of first passage corneal cells were also examined...

..To investigate the expression of p16(INK4a), p21(WAF1/CIP1), p27(KIP1), and p53 in human corneal endothelial cell (HCEC) senescence ex vivo at various donor ages...

..METHODS: The corneal endothelium was investigated in 21 glaucoma patients (29 eyes) who received topical 1% dorzolamide treatment for 3 ..

..To design a technique for transplantation of the Descemet's membrane (DM) as a carrier for its endothelium, while maintaining the low-astigmatic recipient anterior corneal curvature...

..CONCLUSIONS: Hemichannels contribute to ATP release on mechanical stimulation in BCECs. The released ATP contributes to propagation of the Ca(2+) wave...

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..We sought to study HCEC sheet cultivation on such cell culture carriers under serum-free conditions as the next consequential step in developing methods for generation of corneal endothelial cell transplants...

..Inducible rAAV-mediated transgene expression in corneal endothelial cells is a potential strategy in the treatment and prevention of ocular diseases...

..Eyes receiving the expensive combination had marginally faster recovery of corneal swelling by 3 months. However, the cost of Viscoat and 500 mL BSS Plus is 5 times that of Ocucoat and Ringer's solution...

..In this study, blebbistatin, a selective myosin II ATPase inhibitor, was used to investigate whether the effect of thrombin is mediated by enhanced actomyosin contractility...

..These findings further emphasize the role of contractility of the actin cytoskeleton in regulating the barrier integrity of corneal endothelium.

b>Corneal endothelium is the single layer of cells forming a boundary between the corneal stroma and anterior chamber...

..To describe an innovative device that allows gene electrotransfer to human corneal endothelial cells (EC) during storage in organ culture...

Mechanical stimulation induces intercellular Ca(2 +) waves in the corneal endothelium. The extent of the wave propagation is dependent on the activity of gap junctions, hemichannels, and ectonucleotidases...

..To evaluate the visual, topographic, and endothelial survival results in 25 consecutive patients who have received small-incision deep lamellar endothelial keratoplasty (DLEK) transplant surgery...

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..This study demonstrates the presence of Ca(2+) stores in lysosomes of bovine corneal endothelial cells (BCECs) and examines their interaction with Ins(1,4,5)P(3)-sensitive Ca(2+) stores...

..We describe corneal endothelial cell density and morphology in normal Iranian eyes and compare endothelial cell characteristics in the Iranian population with data available in the literature for American and Indian populations...

To investigate the differential expression of the glycoprotein clusterin/apoJ (CLU) in normal and Fuchs' endothelial dystrophy (FED) corneal endothelium and to compare the expression of various forms of CLU in normal and FED tissue.

..To describe genes expressed in human corneal endothelial cells and identify novel genes...

..3% +/- 9.8% in the control group (P =.0003). CONCLUSION: The results suggest that the soft-shell technique is safe and effective in protecting corneal endothelial cells during cataract surgery in patients with a hard lens nucleus...

..To determine the effect of mitomycin-C (MMC) on the cornea after a single intraoperative application...

..To determine how stimuli that increase corneal endothelial cell proliferation of human corneas in culture relate to changes in endothelial cell density in the central and peripheral cornea...

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..To report the 6- and 12-month results of the first United States clinical series of deep lamellar endothelial keratoplasty (DLEK) in the treatment of endothelial dysfunction...

..These findings together suggest that functional gap junctions are present in BCEC, that both GJIC and PIC contribute significantly to IC, and that the two pathways interact...

..Microtubule stabilization may be a useful strategy to overcome (TNF-alpha)-induced loss of the barrier integrity of corneal endothelium during inflammation associated with transplant rejection and uveitis.

..To compare the effectiveness of deep lamellar keratoplasty (DLK) with that of penetrating keratoplasty (PKP) in cases of corneal lesions not involving the endothelium...

..The feasibility of corneal reconstruction with cultured adult human corneal endothelial cells (HCEC) was examined in a nude rat model...

..To compare subbasal corneal nerve and keratocyte density and endothelial characteristics of ocular hypertensive patients treated with medications or observation...

Free-radical formation may play a role in postoperative complications of phacoemulsification (e.g., corneal endothelium damage from mechanical injury)...

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..To evaluate the immunohistochemical characteristics of human corneas with the diagnosis of Fuchs endothelial dystrophy (FED)...

..This technique showed that the appropriately sized amplification products were specific.(ABSTRACT TRUNCATED AT 250 WORDS)..

..Emphasis is given to issues that are contentious, such as the role of bicarbonate in the endothelial pump and the significance of water channels in both corneal limiting cell layers...

To investigate whether modern phacoemulsification surgery results in more damage to the corneal endothelium than extracapsular cataract extraction (ECCE), and to examine which preoperative, operative, and postoperative factors influence ..

..The potential advantages of this procedure over penetrating keratoplasty in the treatment of endothelial dysfunction are considerable...

..Corneal fibrosis is rarely observed in corneal endothelium/Descemet's membrane complex; but when this pathologic tissue occurs, it causes a loss of vision...

..These CECs may secrete the extracellular matrix components found in the RCM. Hsp 47 up-regulation in the CECs may play an important role in RCM formation. These findings provide further insights into the phenotypic modulation of CECs...

..We evaluated the postoperative effect of Viscoat and Healon5 on intraocular pressure (IOP), central corneal thickness (CCT), endothelial cell counts and laser flare...

..To evaluate the feasibility of the femtosecond (FS) laser in preparation of posterior lamellar discs (PLDs) and to study the effect on endothelial cell (EC) viability for Descemet-stripping endothelial keratoplasty...

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..To achieve this, a matrix that can serve as a carrier for cHCECs is needed. The present study was conducted to examine the feasibility of using amniotic membrane (AM) as a carrier for this application...

..If interface clarity can be maintained, the potential advantages over penetrating keratoplasty in the treatment of endothelial dysfunction are considerable...

..To evaluate visual acuity, refractive outcomes, and endothelial cell density 6 months after Descemet stripping and automated endothelial keratoplasty (DSAEK)...

..To evaluate eye bank-prepared tissue for Descemet's stripping automated endothelial keratoplasty (DSAEK)...

..To evaluate ultrasound (US) energy and endothelial cell loss in cataract surgery using the stop-and-chop and nuclear preslice techniques...

..To evaluate visual acuity, topography, and endothelial cell density 1 year after deep lamellar endothelial keratoplasty (DLEK) for endothelial dysfunction...

..We present the results of our clinical series replacing posterior stroma and endothelium only by deep lamellar endothelial keratoplasty (DLEK) in patients with corneal endothelial diseases...

..To report the outcomes in eyes in which a Sinskey-type conventional posterior chamber intraocular lens (PC-IOL) was fixed in the anterior chamber and followed up for an average of 10 years...

..Deep lamellar endothelial keratoplasty is, therefore, an excellent alternative to PK for patients with endothelial dystrophies...

..To report and discuss the early postoperative complications of deep lamellar endothelial keratoplasty (DLEK)...

..To present a new surgical technique combining penetrating keratoplasty and open-sky posterior iris fixation of the Artisan iris-claw intraocular lens (IOL) for treatment of pseudophakic bullous keratopathy in a case series of five patients...

..To compare the outcomes of bimanual microphacoemulsification and coaxial miniphacoemulsification and assess the potential advantages of the former over the latter...

..This case highlights that a healthy endothelium can maintain corneal deturgescence despite a low endothelial cell count. Corneal thickness increases only when the number of endothelial cells has gone below a physiologic lower limit...

..To compare the therapeutic outcomes after deep lamellar keratoplasty (DLKP) and penetrating keratoplasty (PKP) in patients with lattice corneal dystrophy (LCD) and macular corneal dystrophy (MCD)...

..To determine the safety of prophylactic intracameral moxifloxacin 0.5% ophthalmic solution (Vigamox) in patients having cataract surgery...

..To assess the clinical benefit, relative efficacy, and pharmacokinetic-response curve of preoperative and postoperative ketorolac tromethamine 0.4% (Acular LS) to improve outcomes during and after cataract surgery...

..To compare clinical outcomes of biaxial microincision (1.5 mm) and coaxial small-incision (2.75 mm) clear cornea cataract surgery...

..Collagen is arranged well at the surface of the lamellar. Fibrotic repair is seen in the marginal area connecting the donor disc and recipient's bed...

..To compare the surgical trauma after microincision phacoemulsification and small-incision coaxial phacoemulsification after implantation of conventional, foldable, hydrophobic acrylic intraocular lenses (IOLs)...

..001) on the first postoperative day. CONCLUSIONS: The prevalence of RAM was 6%. Relative anterior microphthalmos with its associations posed significant intraoperative difficulties. The occurrence of transient corneal edema was frequent...

..This study was designed to report the use of deep lamellar endothelial keratoplasty (DLEK) in combination with other intraocular surgeries in the treatment of eyes with severe bullous keratopathy and visual loss...

..The long-term effect of this adverse event was assessed...

..It compares favorably with sodium hyaluronate 1% and can be used as an effective and cheaper alternative in routine small incision cataract surgery with implant...

..To investigate the efficacy of a new surgical and rehabilitative procedure designed to improve vision in patients with central scotoma due to macular diseases...

..To evaluate the functional and endothelial prognosis after Descemet's membrane perforation during deep anterior lamellar keratoplasty (DALK)...

..We report 2 cases in which a Verisyse phakic intraocular lens (PIOL) was used to successfully treat high myopia after penetrating keratoplasty (PK) in pseudophakic patients...

..To report 1-year results of laser in situ keratomileusis (LASIK) in 7 eyes with corneal endothelial guttata and a family history of Fuchs' endothelial dystrophy...

..To assess the endothelial cell loss after cataract extraction using a fluid-based system and compare it with conventional ultrasound...

..To evaluate the long-term results of air-guided manual deep anterior lamellar keratoplasty (DALK) and to perform confocal microscopy on postoperative DALK corneas...

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..There is no compression of tissue so mechanical trauma to the endothelium is less than when an inserting forceps is used. The results in 12 cases are presented...

..To evaluate posterior and anterior segment safety of an intracameral injection of moxifloxacin 0.5% ophthalmic solution as prophylaxis for endophthalmitis in patients having cataract surgery...

..To evaluate the use of the Femtec femtosecond (fs) laser for penetrating keratoplasty (PK) in the treatment of corneal diseases...

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..To compare endothelial cell damage during cataract surgery performed using the divide-and-conquer or phaco-chop nuclear fracturing technique...

..There is mounting evidence that oxidative stress induces damage to corneal endothelium in FECD...

..fibroblastic cell populations differentiate into distinct cell-types in the eye, including corneal nerves, corneal endothelium, stromal keratocytes, non-myelinating and myelinating Schwann cells, connective tissues of limbus, ..

..null mutant mice for Foxc1 have numerous ocular abnormalities, including lack of the anterior chamber and corneal endothelium, iris dystrophy and abnormalities of the trabecular meshwork...

..fibroblastic cell populations differentiate into distinct cell-types in the eye, including corneal nerves, corneal endothelium, stromal keratocytes, non-myelinating and myelinating Schwann cells, connective tissues of limbus, ..

DESCRIPTION: Retrocorneal fibrous membrane (RCFM) occurs in various corneal diseases or after damage to the corneal endothelium. RCFM of Descemet's membrane is believed to represent an end-stage disease process of the corneal ..

..The understanding to be gained may provide insights on how to modulate fluid transport so as to offset endothelial dysfunction leading to the loss of normal vision. ..

..to test the following hypothesis about the specific effects of each of three factors on the compromised corneal endothelium. (1) that the corneal endothelium has a regeneration zone (stem cells) that maintains a constant supply of ..

..research; 1) To characterize the morphologic and functional changes involved in disorders of the human corneal endothelium and 2) to develop a method for permanent corneal storage to improve the overall results of corneal ..

..the following specific aims: (1) Is polymegathism related to corneal endothelial cytoskeleton change? The corneal endothelium of contact lens wearers, diabetic, age related polymegathism and following wound healing will be studied...

..The long-term goal of this project is to understand how the corneal endothelium does this job, so that medical therapies can be developed to enhance endothelial function in corneas that ..

..The long-term goal of this project is to understand how the corneal endothelium does this job, so that medical therapies can be developed to enhance endothelial function in corneas that ..

..The long-term goal of this project is to understand how the corneal endothelium does this job, so that medical therapies can be developed to enhance endothelial function in corneas that ..

DESCRIPTION (applicant's abstract): Disease and damage at the level of Descemet's membrane/corneal endothelium can result in production of retrocorneal fibrous membrane (RCFM)...

..3) Characterize the properties of individual ion channels in corneal endothelium and corneal epithelium...

..of periocular neural crest into the eye rudiment, their interaction with the lens and differentiation into corneal endothelium and stroma, and relationship to neural crest-derived keratocytes during regeneration of the cornea...

..shown expression of AQP1 and AQP4 in non-pigmented ciliary epithelium, AQP1 in canal of Schlemm, AQP1 in corneal endothelium and AQP5 in corneal epithelium...

..Transparency is dependent on corneal hydration, which is controlled by an epithelial cell layer called the corneal endothelium. The endothelium controls hydration by secreting fluid from the corneal stoma to the aqueous humor by ion ..

Disease and damage at the level of Descemet's membrane/corneal endothelium can result in production of retrocorneal fibrous membrane (RCFM)...

Disease and damage at the level of Descemet's membrane/corneal endothelium can result in production of retrocorneal fibrous membrane (RCFM)...

..as commonly done with other water transporting epithelia such as those of the kidney, urinary bladder and corneal endothelium. Our preliminary, experiments show that we can measure fluid transport in this isolated tissue...