limbus corneae

Summary

Summary: An annular transitional zone, approximately 1 mm wide, between the cornea and the bulbar conjunctiva and sclera. It is highly vascular and is involved in the metabolism of the cornea. It is ophthalmologically significant in that it appears on the outer surface of the eyeball as a slight furrow, marking the line between the clear cornea and the sclera. (Dictionary of Visual Science, 3d ed)

Top Publications

  1. doi Limbal stem-cell therapy and long-term corneal regeneration
    Paolo Rama
    San Raffaele Scientific Institute, Ophthalmology Unit, Milan, Italy
    N Engl J Med 363:147-55. 2010
  2. ncbi Characterization of the limbal epithelial stem cell niche: novel imaging techniques permit in vivo observation and targeted biopsy of limbal epithelial stem cells
    Alex J Shortt
    MRC Clinical Research Fellow, Institute of Ophthalmology and Moorfields Eye Hospital, 11 43 Bath Street, London, EC1V 9EL, United Kingdom
    Stem Cells 25:1402-9. 2007
  3. doi Clinical outcomes of xeno-free autologous cultivated limbal epithelial transplantation: a 10-year study
    Virender S Sangwan
    Cornea and Anterior Segment Services, L V Prasad Eye Institute, Kallam Anji Reddy Campus, Road No 2, Banjara Hills, Hyderabad 500034, India
    Br J Ophthalmol 95:1525-9. 2011
  4. doi Ex vivo expansion and transplantation of limbal epithelial stem cells
    Alex J Shortt
    Cells for Sight Transplantation and Research Programme, UCL Institute of Ophthalmology, London, United Kingdom
    Ophthalmology 115:1989-97. 2008
  5. ncbi Amniotic membrane use in ophthalmology
    Jose A P Gomes
    Advanced Ocular Surface Center, Brazil
    Curr Opin Ophthalmol 16:233-40. 2005
  6. ncbi The phenotype of limbal epithelial stem cells
    Edwin C Figueira
    Inflammatory Diseases Research Unit, University of New South Wales, Australia
    Invest Ophthalmol Vis Sci 48:144-56. 2007
  7. pmc ABCG2-dependent dye exclusion activity and clonal potential in epithelial cells continuously growing for 1 month from limbal explants
    Ozlem Barut Selver
    Department of Ophthalmology, Dokuz Eylul University, Izmir, Turkey
    Invest Ophthalmol Vis Sci 52:4330-7. 2011
  8. doi Characterization of limbal stem cell deficiency by in vivo laser scanning confocal microscopy: a microstructural approach
    Sophie X Deng
    Jules Stein Eye Institute, 100 Stein Plaza, Los Angeles, CA 90095, USA
    Arch Ophthalmol 130:440-5. 2012
  9. pmc Comparative transcriptional profiling of the limbal epithelial crypt demonstrates its putative stem cell niche characteristics
    Bina B Kulkarni
    Division of Ophthalmology and Visual Sciences, Eye and ENT Building Queen s Medical Centre, Derby Road, Nottingham, UK
    BMC Genomics 11:526. 2010
  10. pmc Isolation and expansion of human limbal stromal niche cells
    Hua Tao Xie
    R and D Department, TissueTech, Inc, Ocular Surface Center, and Ocular Surface Research and Education Foundation, Miami, Florida 33173, USA
    Invest Ophthalmol Vis Sci 53:279-86. 2012

Research Grants

  1. Isolation of Limbal Epithelial Stem Cells
    ROBERT LAVKER; Fiscal Year: 2003
  2. SERINE PROTEINASE INHIBITORS IN OCULAR SURFACE EPITHELIA
    ROBERT LAVKER; Fiscal Year: 2003
  3. CORNEAL EPITHELIAL STEM CELLS
    ROBERT LAVKER; Fiscal Year: 2004
  4. CORNEAL EPITHELIAL STEM CELLS
    ROBERT LAVKER; Fiscal Year: 2006
  5. CORNEAL EPITHELIAL STEM CELLS
    ROBERT LAVKER; Fiscal Year: 2009
  6. OCULAR SURFACE EPITHELIAL PRECURSORS
    JOSE WOLOSIN; Fiscal Year: 2007
  7. Gene Gun Technology, Opioids, and Corneal Diseases
    IAN ZAGON; Fiscal Year: 2003
  8. 4th International Research Workshop on Alopecia Areata
    ROBERT LAVKER; Fiscal Year: 2002
  9. CELL MEMBRANE DIFFERENTIATION IN CORNEAL EPITHELIUM
    JOSE WOLOSIN; Fiscal Year: 2002
  10. OCULAR SURFACE EPITHELIAL PRECURSORS
    JOSE WOLOSIN; Fiscal Year: 2009

Detail Information

Publications216 found, 100 shown here

  1. doi Limbal stem-cell therapy and long-term corneal regeneration
    Paolo Rama
    San Raffaele Scientific Institute, Ophthalmology Unit, Milan, Italy
    N Engl J Med 363:147-55. 2010
    ..We investigated the long-term clinical results of cell therapy in patients with burn-related corneal destruction associated with limbal stem-cell deficiency, a highly disabling ocular disease...
  2. ncbi Characterization of the limbal epithelial stem cell niche: novel imaging techniques permit in vivo observation and targeted biopsy of limbal epithelial stem cells
    Alex J Shortt
    MRC Clinical Research Fellow, Institute of Ophthalmology and Moorfields Eye Hospital, 11 43 Bath Street, London, EC1V 9EL, United Kingdom
    Stem Cells 25:1402-9. 2007
    ..Disclosure of potential conflicts of interest is found at the end of this article...
  3. doi Clinical outcomes of xeno-free autologous cultivated limbal epithelial transplantation: a 10-year study
    Virender S Sangwan
    Cornea and Anterior Segment Services, L V Prasad Eye Institute, Kallam Anji Reddy Campus, Road No 2, Banjara Hills, Hyderabad 500034, India
    Br J Ophthalmol 95:1525-9. 2011
    ..Ocular burns can damage the corneal epithelial stem cells located at the limbus. This study evaluated the efficacy of xeno-free autologous cell-based treatment of limbal stem cell deficiency...
  4. doi Ex vivo expansion and transplantation of limbal epithelial stem cells
    Alex J Shortt
    Cells for Sight Transplantation and Research Programme, UCL Institute of Ophthalmology, London, United Kingdom
    Ophthalmology 115:1989-97. 2008
    ..To determine, using objective measures, the outcome of ex vivo cultured limbal epithelial stem cell (LESC) transplantation performed in compliance with good manufacturing practice using a novel culture system without 3T3 feeder cells...
  5. ncbi Amniotic membrane use in ophthalmology
    Jose A P Gomes
    Advanced Ocular Surface Center, Brazil
    Curr Opin Ophthalmol 16:233-40. 2005
    ..The purpose of this review is to describe the most recent and relevant clinical and experimental data about the use of amniotic membrane in ophthalmology...
  6. ncbi The phenotype of limbal epithelial stem cells
    Edwin C Figueira
    Inflammatory Diseases Research Unit, University of New South Wales, Australia
    Invest Ophthalmol Vis Sci 48:144-56. 2007
    ..The purpose of this study was to identify phenotypic markers of human limbal stem cells in fetal and adult corneas...
  7. pmc ABCG2-dependent dye exclusion activity and clonal potential in epithelial cells continuously growing for 1 month from limbal explants
    Ozlem Barut Selver
    Department of Ophthalmology, Dokuz Eylul University, Izmir, Turkey
    Invest Ophthalmol Vis Sci 52:4330-7. 2011
    ..To determine changes in ABCG2-transport-dependent dye exclusion in outgrowths from limbal explants...
  8. doi Characterization of limbal stem cell deficiency by in vivo laser scanning confocal microscopy: a microstructural approach
    Sophie X Deng
    Jules Stein Eye Institute, 100 Stein Plaza, Los Angeles, CA 90095, USA
    Arch Ophthalmol 130:440-5. 2012
    ..To evaluate the cellular changes in the corneal epithelium and surrounding structures in limbal stem cell deficiency (LSCD) by using in vivo laser scanning confocal microscopy...
  9. pmc Comparative transcriptional profiling of the limbal epithelial crypt demonstrates its putative stem cell niche characteristics
    Bina B Kulkarni
    Division of Ophthalmology and Visual Sciences, Eye and ENT Building Queen s Medical Centre, Derby Road, Nottingham, UK
    BMC Genomics 11:526. 2010
    ..The purpose of this microarray study was to characterise the transcriptional profile of the LEC and compare with other ocular surface epithelial regions to support our hypothesis that LEC preferentially harbours stem cells (SC)...
  10. pmc Isolation and expansion of human limbal stromal niche cells
    Hua Tao Xie
    R and D Department, TissueTech, Inc, Ocular Surface Center, and Ocular Surface Research and Education Foundation, Miami, Florida 33173, USA
    Invest Ophthalmol Vis Sci 53:279-86. 2012
    ..Limbal stromal niche cells heterogeneously express embryonic stem cell (SC) markers. This study was conducted to isolate and expand them and to prove that their phenotype is critical for supporting SCs...
  11. pmc Epithelial cell characteristics of cultured human limbal explants
    A Joseph
    Larry A Donoso Laboratory for Eye Research, Division of Ophthalmology and Visual Sciences, University of Nottingham, Nottingham NG7 2UH, UK
    Br J Ophthalmol 88:393-8. 2004
    ..To determine the immunohistochemical characteristics of putative corneal epithelial stem cells remaining on limbal explants maintained in culture...
  12. ncbi In search of markers for the stem cells of the corneal epithelium
    Ahdeah Pajoohesh-Ganji
    Department of Anatomy and Cell Biology, The George Washington University Medical Center, Washington, DC, USA
    Biol Cell 97:265-76. 2005
    ..In this review, we summarize the current state of knowledge of the ocular surface and its adult stem cells, and analyse data as they now exist regarding putative corneal epithelial stem cell markers...
  13. pmc S100A expression in normal corneal-limbal epithelial cells and ocular surface squamous cell carcinoma tissue
    Jing Li
    Department of Ophthalmology, Xinhua Hospital, Shanghai Jiao Tong University, China
    Mol Vis 17:2263-71. 2011
    ..To study the expression and cellular distribution of multiple S100A genes and proteins in normal corneal-limbal epithelium and ocular surface squamous cell carcinoma (SCC) tissue...
  14. pmc ABCG2 transporter identifies a population of clonogenic human limbal epithelial cells
    Cintia S de Paiva
    Ocular Surface Center, Cullen Eye Institute, Department of Ophthalmology, Baylor College of Medicine, 6565 Fannin St, NC 205, Houston, Texas 77030, USA
    Stem Cells 23:63-73. 2005
    ....
  15. ncbi Clinical and cytological findings in limbal stem cell deficiency
    Marta Sacchetti
    Interdisciplinary Center for Biomedical Research CIR Laboratory of Ophthalmology, University of Rome Campus Bio Medico, Rome, Italy
    Graefes Arch Clin Exp Ophthalmol 243:870-6. 2005
    ..To evaluate and relate the clinical (including corneal sensitivity and tear function) and cytological (presence of goblet cells and cytokeratin 3- and 19-positive cells) features of limbal stem cell deficiency (LSCD)...
  16. doi Enhanced functional properties of corneal epithelial cells by coculture with embryonic stem cells via the integrin β1-FAK-PI3K/Akt pathway
    Jin Zhou
    State Key Laboratory of Ophthalmology, Sun Yet Sen University, Guangzhou, Guangdong, PR China
    Int J Biochem Cell Biol 43:1168-77. 2011
    ..These findings demonstrated that the functional properties of stem-like corneal epithelial cells were enhanced by cocultured embryonic stem cells via activation of the integrin β1-FAK-PI3K/Akt signalling pathway...
  17. doi Ex vivo expanded autologous limbal epithelial cells on amniotic membrane using a culture medium with human serum as single supplement
    Aboulghassem Shahdadfar
    Center for Eye Research, Department of Ophthalmology, Oslo University Hospital and University of Oslo, Norway
    Exp Eye Res 97:1-9. 2012
    ....
  18. pmc p63 identifies keratinocyte stem cells
    G Pellegrini
    Laboratory of Tissue Engineering IDI, Istituto Dermopatico dell Immacolata, 00040 Rome, Italy
    Proc Natl Acad Sci U S A 98:3156-61. 2001
    ..The identification of p63 as a keratinocyte stem cell marker will be of practical importance for the clinical application of epithelial cultures in cell therapy as well as for studies on epithelial tumorigenesis...
  19. doi Preservation of the limbal stem cell phenotype by appropriate culture techniques
    Ewa Anna Meyer-Blazejewska
    Department of Ophthalmology, Friedrich Alexander University of Erlangen Nurnberg, Erlangen, Germany
    Invest Ophthalmol Vis Sci 51:765-74. 2010
    ....
  20. pmc Niche regulation of corneal epithelial stem cells at the limbus
    Wei Li
    Ocular Surface Center and TissueTech Inc Miami, Florida, 33173, USA
    Cell Res 17:26-36. 2007
    ....
  21. pmc Corneal limbal microenvironment can induce transdifferentiation of hair follicle stem cells into corneal epithelial-like cells
    Ewa Anna Blazejewska
    Department of Ophthalmology, University of Erlangen Nuremberg, Germany
    Stem Cells 27:642-52. 2009
    ..Therefore, the HF may be an easily accessible alternative therapeutic source of autologous adult stem cells for replacement of the corneal epithelium and restoration of visual function in patients with ocular surface disorders...
  22. pmc Partial enrichment of a population of human limbal epithelial cells with putative stem cell properties based on collagen type IV adhesiveness
    De Quan Li
    Ocular Surface Center, Cullen Eye Institute, Department of Ophthalmology, Baylor College of Medicine, 6565 Fannin Street, NC 205, Houston, TX 77030, USA
    Exp Eye Res 80:581-90. 2005
    ..The RAC population enriched for certain putative stem cell properties may prove useful in the future for transplantation to diseased and damaged corneas with limbal stem cell deficiency...
  23. ncbi Analysis of limbal stem cell deficiency by corneal impression cytology
    Pietro Maria Donisi
    Department of Pathology, General Hospital, Venezia, Italy
    Cornea 22:533-8. 2003
    ....
  24. ncbi Limbal stem cells of the corneal epithelium
    H S Dua
    Queen s Medical Centre, University of Nottingham, Nottingham, UK
    Surv Ophthalmol 44:415-25. 2000
    ..With the latter option, systemic immunosuppression is required. Amniotic membrane transplantation is a useful adjunct to the above procedures in some instances...
  25. doi Acute wound healing in the human central corneal epithelium appears to be independent of limbal stem cell influence
    Chuan Yuan Chang
    Department of Ophthalmology, Faculty of Medical and Health Sciences, University of Auckland, Auckland, New Zealand
    Invest Ophthalmol Vis Sci 49:5279-86. 2008
    ..These migrate centripetally, differentiate outward to the surface, and are then lost by desquamation. This study was conducted to analyze the contribution of human central corneal epithelial cells toward corneal epithelial regeneration...
  26. ncbi Characterization of extracellular matrix components in the limbal epithelial stem cell compartment
    U Schlötzer-Schrehardt
    Department of Ophthalmology, University of Erlangen Nurnberg, Schwabachanlage 6, D 91054 Erlangen, Germany
    Exp Eye Res 85:845-60. 2007
    ..Identification of specific niche parameters might not only help to understand limbal stem cell regulation, but also to improve their selective enrichment and in vitro expansion for therapeutic strategies...
  27. pmc Limbal epithelial crypts: a novel anatomical structure and a putative limbal stem cell niche
    H S Dua
    The Larry A Donoso Laboratory for Eye Research, Division of Ophthalmology and Visual Sciences, University of Nottingham, UK
    Br J Ophthalmol 89:529-32. 2005
    ..The authors undertook to re-evaluate the microanatomy of the limbus in order to identify possible sites that may represent a stem cell niche...
  28. doi Corneal epithelial stem cells: deficiency and regulation
    Genevieve A Secker
    Cells for Sight Transplantation and Research Programme, Ocular Repair and Regeneration Biology Unit, Division of Pathology, UCL Institute of Ophthalmology, 11 43 Bath Street, London, EC1V 9EL, UK
    Stem Cell Rev 4:159-68. 2008
    ..Developments in Biologicals 222:41-54, 2000). Studies of models of Pax6 deficiency, such as the small eyed (sey) mouse, should help to reveal the intrinsic and extrinsic mechanisms involved in normal LESC function...
  29. pmc Stratified epithelial sheets engineered from a single adult murine corneal/limbal progenitor cell
    Tetsuya Kawakita
    TissueTech, Inc, and Ocular Surface Center, Miami, FL 33173, USA
    J Cell Mol Med 12:1303-16. 2008
    ....
  30. pmc Expression of toll-like receptors in human limbal and conjunctival epithelial cells
    Jing Li
    Singapore Eye Research Institute, Department of Ophthalmology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore
    Mol Vis 13:813-22. 2007
    ..To determine the expression and function of toll-like receptors (TLRs) in human conjunctival, limbal and corneal epithelial cells...
  31. pmc Preferential gene expression in the limbus of the vervet monkey
    Zhenhua Ding
    Cornea and Uveitis Division, Jules Stein Eye Institute, University of California, Los Angeles, CA 90095, USA
    Mol Vis 14:2031-41. 2008
    ..To elucidate the unique molecular factors and biological processes that are differentially expressed in the limbal stem cell microenvironment by comparing directly to that of its immediate adjacent structures, the cornea and conjunctiva...
  32. doi The role of limbal stem cells in corneal epithelial maintenance: testing the dogma
    Harminder S Dua
    Division of Ophthalmology and Visual Sciences, Queens Medical Centre, University Hospital, Derby Road, Nottingham, UK
    Ophthalmology 116:856-63. 2009
    ....
  33. ncbi Proteomic analysis of epithelium-denuded human amniotic membrane as a limbal stem cell niche
    Hossein Baharvand
    Department of Stem Cells, Royan Institute, Tehran, Iran
    Mol Vis 13:1711-21. 2007
    ..We investigated the proteome of the epithelium-denuded AM to increase our understanding of the mechanisms by which AM may confer its beneficial effects...
  34. ncbi The transdifferentiation potential of limbal fibroblast-like cells
    Subhadra Dravida
    Embryonic and Ocular Stem Cell Group, Reliance Life Sciences Pvt Ltd, Dhirubhai Ambani Life Sciences Centre, R 282, TTC Area of MIDC, Thane Belapur Road, Rabale, Navi Mumbai 400 701, India
    Brain Res Dev Brain Res 160:239-51. 2005
    ..This multipotential capability, relatively easy isolation and high rate of ex vivo proliferation capacity make these cells a promising therapeutic tool...
  35. doi 13 years of cultured limbal epithelial cell therapy: a review of the outcomes
    Oliver Baylis
    Institute of Human Genetics, Newcastle University, Newcastle upon Tyne, UK
    J Cell Biochem 112:993-1002. 2011
    ..Here, we review the outcomes of this procedure over the past 13 years with the aim of highlighting the best culture and surgical techniques to date...
  36. pmc Cultivation and characterization of cornea limbal epithelial stem cells on lens capsule in animal material-free medium
    Reka Albert
    Department of Ophthalmology, Medical and Health Science Center, University of Debrecen, Hungary
    PLoS ONE 7:e47187. 2012
    ....
  37. doi Hypoxia is a key regulator of limbal epithelial stem cell growth and differentiation
    Chris Bath
    Department of Ophthalmology, Aalborg University Hospital, 9100 Aalborg, and Laboratory for Stem Cell Research, Aalborg University, 9220 Aalborg, Denmark
    Stem Cell Res 10:349-60. 2013
    ..Hypoxic cultures may thus potentially improve stem cell grafts for cultured limbal epithelial transplantation (CLET)...
  38. pmc Human epithelial cell cultures from superficial limbal explants
    D Ghoubay-Benallaoua
    Institut de la Vision, UPMC Univ Paris 06, UMR_S 968 INSERM, U968 CHNO des XV XX CNRS, UMR_7210, Paris, France
    Mol Vis 17:341-54. 2011
    ..To study the kinetics of growth and the phenotype of cells cultured from human limbal explants in a cholera toxin-free medium with no feeder cell layer...
  39. ncbi [Transplantation of bioengineered corneal epithelium for the treatment of total limbal stem cell deficiency in rabbit]
    Qi Lin
    Beijing Tongren Ophthalmic Center, Capital University of Medical Sciences, Beijing 100730, China
    Zhonghua Yan Ke Za Zhi 42:679-85. 2006
    ..To study the results of transplantation of cultured autologous limbal stem cells using fibrin gel membrane as substrate for the treatment of limbal stem cell deficiency in rabbits...
  40. doi Optimization of culture conditions for an efficient xeno-feeder free limbal cell culture system towards ocular surface regeneration
    Viji Mary Varghese
    Tissue Culture Laboratory, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Thiruvananthapuram, Kerala, India
    Microsc Res Tech 73:1045-52. 2010
    ..This study will be a major step in the development of xeno-feeder free epithelial equivalents towards ocular surface reconstruction...
  41. doi Silk fibroin as a biomaterial substrate for corneal epithelial cell sheet generation
    Jingbo Liu
    Weill Cornell Medical College, New York, New York, USA
    Invest Ophthalmol Vis Sci 53:4130-8. 2012
    ..To evaluate a silk fibroin (SF) biomaterial as a substrate for corneal epithelial cell proliferation, differentiation, and stratification in vitro compared with denuded human amniotic membrane (AM)...
  42. ncbi Limbal relaxing incisions versus on-axis incisions to reduce corneal astigmatism at the time of cataract surgery
    Claude Kaufmann
    Department of Ophthalmology, University of Zurich, Zurich, Switzerland
    J Cataract Refract Surg 31:2261-5. 2005
    ..To compare limbal relaxing incisions (LRIs) with placement of the corneal cataract incision on the steepest keratometric axis for the reduction of preexisting corneal astigmatism at the time of cataract surgery...
  43. doi Contemporary limbal stem cell transplantation - a review
    Harminder S Dua
    Division of Ophthalmology and Visual Sciences, University of Nottingham, Nottingham NG7 2UH, England, UK
    Clin Experiment Ophthalmol 38:104-17. 2010
    ..Despite its complexities limbal transplantation does significantly improve vision related quality of life. Autografts give the best results and living related donor grafts are next best. Majority of cadaver grafts fail in 5 years...
  44. ncbi [Importance of 3T3 feeder layer to establish epithelial cultures from cell suspension obtained from corneo-scleral rims]
    Priscila Cardoso Cristovam
    Instituto da Visão, Centro Avançado de Superfície Ocular, Universidade Federal de Sao Paulo, Sao Paulo, Brasil
    Arq Bras Oftalmol 71:689-94. 2008
    ..To evaluate the importance of the presence of 3T3 fibroblasts for establishing limbal epithelial cultures from cell suspension obtained from corneo-scleral rims (CSR)...
  45. ncbi The side population cells in the rabbit limbus sensitively increased in response to the central cornea wounding
    Ki Sook Park
    R and D Institute, Modern Cell and Tissue Technologies, Inc, Seoul, Korea
    Invest Ophthalmol Vis Sci 47:892-900. 2006
    ..This study was performed to demonstrate the presence and the characteristics of SP cells in the rabbit limbal epithelium and explore their sensitivity in response to the central cornea wounding...
  46. ncbi Homologous penetrating central limbo-keratoplasty (HPCLK) in bilateral limbal stem cell insufficiency
    T Reinhard
    Eye Clinic, Heinrich Heine University, Dusseldorf, Germany
    Acta Ophthalmol Scand 77:663-7. 1999
    ..We have four years' experience with a new surgical one-stage procedure, homologous penetrating central limbo-keratoplasty (HPCLK)...
  47. doi In sickness and in health: Corneal epithelial stem cell biology, pathology and therapy
    M Notara
    Department of Ocular Biology and Therapeutics, UCL Institute of Ophthalmology, Cells for Sight Transplantation and Research Programme, 11 43 Bath Street, London EC1V 9EL, UK
    Exp Eye Res 90:188-95. 2010
    ..The clinical precedence for use of stem cell therapy and the accessibility of the transparent stem cell niche make the cornea a unique model for the study of adult stem cells in physiological conditions as well as in disease...
  48. ncbi Corneal development, limbal stem cell function, and corneal epithelial cell migration in the Pax6(+/-) mouse
    J Martin Collinson
    Department of Biomedical Sciences, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen, Scotland, United Kingdom
    Invest Ophthalmol Vis Sci 45:1101-8. 2004
    ..To investigate the etiology of corneal dysfunction in the Pax6(+/-) mouse model of aniridia-related keratopathy...
  49. doi Tissue-regenerating, vision-restoring corneal epithelial stem cells
    Timothy Jerome Echevarria
    Inflammation and Infection Research Centre, Department of Pathology, School of Medical Sciences, University of New South Wales, Sydney, 2052, Australia
    Stem Cell Rev 7:256-68. 2011
    ..Determining where donor stem cells reside on the recipient's ocular surface and how long they remain viable will provide further insights into improving current therapeutic options for patients with LSCD...
  50. doi N-cadherin in the maintenance of human corneal limbal epithelial progenitor cells in vitro
    Kazunari Higa
    Cornea Center, Tokyo Dental College, Chiba, Japan
    Invest Ophthalmol Vis Sci 50:4640-5. 2009
    ..To demonstrate the role of N-cadherin (N-cad) in maintaining the progenitor status of primary human limbal epithelial cells in vitro...
  51. ncbi Survival of cultured allogeneic limbal epithelial cells following corneal repair
    Justin R Sharpe
    Blond McIndoe Centre, Queen Victoria Hospital, East Grinstead, and Department of Pharmacy and Biomolecular Sciences, University of Brighton, UK
    Tissue Eng 13:123-32. 2007
    ....
  52. doi IL6 and the human limbal stem cell niche: a mediator of epithelial-stromal interaction
    Maria Notara
    Department of Ocular Biology and Therapeutics, UCL Institute of Ophthalmology, London, UK
    Stem Cell Res 5:188-200. 2010
    ..STAT3 and IL6 inhibition in 3.1SF cultures significantly reduced HLE colony-forming efficiency, suggesting a previously undetected STAT3-mediated involvement of IL6 in the maintenance of HLE cells in a progenitor-like state...
  53. doi New hopes and strategies for the treatment of severe ocular surface disease
    Takahiro Nakamura
    Department of Ophthalmology, Graduate School of Medicine, Kyoto Prefectural University of Medicine, Kyoto, Japan
    Curr Opin Ophthalmol 22:274-8. 2011
    ..This article aims to describe the recent advances and current development of ocular surface reconstruction from both basic science and clinical aspects...
  54. ncbi Full-thickness central corneal grafts in lamellar keratoscleroplasty to treat limbal dermoids
    Yun Dun Shen
    Department of Ophthalmology, National Taiwan University Hospital, Taipei, Taiwan
    Ophthalmology 112:1955. 2005
    ..To assess the surgical outcomes in patients who underwent lamellar keratoscleroplasty with full-thickness central corneal grafts for limbal dermoids...
  55. ncbi Outcome of lamellar keratoplasty for limbal dermoids in children
    Patrick Watts
    Department of Ophthalmology, The Hospital for Sick Children, Toronto, Ontario, Canada
    J AAPOS 6:209-15. 2002
    ..We report on the surgical management, complications, postoperative visual acuity, and astigmatism of limbal dermoids in children...
  56. ncbi The spectrum of cytokeratins expressed in the adult human cornea, limbus and perilimbal conjunctiva
    Stanislava Merjava
    Laboratory of the Biology and Pathology of the Eye, Institute of Inherited Metabolic Disorders, General Teaching Hospital and Charles University, Prague, Czech Republic
    Histol Histopathol 26:323-31. 2011
    ..The detailed characterization of the corneal, limbal and perilimbal conjunctival epithelium under normal circumstances may be useful for characterizing the changes occurring under pathological conditions...
  57. doi The porcine limbal epithelial stem cell niche as a new model for the study of transplanted tissue-engineered human limbal epithelial cells
    Maria Notara
    Ocular Biology and Therapeutics, UCL Institute of Ophthalmology, London, United Kingdom
    Tissue Eng Part A 17:741-50. 2011
    ..These data have significant clinical value as this model can provide information on LESC fate post-transplantation and their ability to respond to injury, which is not possible to study in patients...
  58. doi Effects of fibroblast origin and phenotype on the proliferative potential of limbal epithelial progenitor cells
    S Louise Ainscough
    Discipline of Medical Sciences and Vision Improvement Domain, Institute of Health and Biomedical Innovation, Queensland University of Technology, 60 Musk Avenue, Kelvin Grove, Brisbane, QLD 4059, Australia
    Exp Eye Res 92:10-9. 2011
    ..Superior growth of LEPs in the presence of limbal fibroblasts indicates a role for limbal fibroblasts in promoting the proliferation of limbal epithelium during wound healing...
  59. ncbi Corneal epithelial cultures generated from organ-cultured limbal tissue: factors influencing epithelial cell growth
    Eleonora Zito-Abbad
    Cornea Bank of EFS Ile de France and Department of Ophthalmology, centre hospitalier national d ophtalmologie des XV XX, Paris VI University, 28 rue de Charenton, 75012 Paris, France
    Curr Eye Res 31:391-9. 2006
    ..To explore the in vitro proliferative potential of human limbal epithelial cells after 31 degrees C organ-culture storage and to investigate putative factors influencing it...
  60. doi A systematic literature review of surgical interventions for limbal stem cell deficiency in humans
    Paul A Cauchi
    Department of Ophthalmology, Aberdeen Royal Infirmary, University of Aberdeen, Aberdeen, United Kingdom
    Am J Ophthalmol 146:251-259. 2008
    ..To evaluate the relative benefits and to identify any adverse effects of surgical interventions for limbal stem cell deficiency (LSCD)...
  61. doi Cytokeratin 8 is expressed in human corneoconjunctival epithelium, particularly in limbal epithelial cells
    Stanislava Merjava
    Laboratory of the Biology and Pathology of the Eye, Institute of Inherited Metabolic Disorders, General Teaching Hospital and First Faculty of Medicine, Charles University, Unemocnice 2, 128 08, Prague, Czech Republic
    Invest Ophthalmol Vis Sci 52:787-94. 2011
    ..The purpose of this study was to investigate the expression of cytokeratin (CK) 8 in the corneoconjunctival epithelium...
  62. doi Comparison of the histology, gene expression profile, and phenotype of cultured human limbal epithelial cells from different limbal regions
    Tor P Utheim
    Center for Clinical Research, Oslo University Hospital, Ulleval, University of Oslo, Oslo, Norway
    Invest Ophthalmol Vis Sci 50:5165-72. 2009
    ..To investigate whether human limbal epithelial cells (HLECs) derived from various regions of the limbus exhibit differences in gene expression and epithelial characteristics...
  63. ncbi Therapeutic lamellar keratoplasty for limbal dermoids
    J A Scott
    Singapore National Eye Centre, Singapore
    Ophthalmology 108:1858-67. 2001
    ..However, improvement in refractive, astigmatic, and visual status is not guaranteed, and these procedures should be viewed more cautiously, although established good vision was maintained in our series...
  64. ncbi Limbal relaxing incisions for primary mixed astigmatism and mixed astigmatism after cataract surgery
    H üseyin Bayramlar H
    Inonu University, Turgut Ozal Medical Center, Department of Ophthalmology, Malatya, Turkey
    J Cataract Refract Surg 29:723-8. 2003
    ..To evaluate the effect of limbal relaxing incisions (LRIs) in the treatment of primary mixed astigmatism and mixed astigmatism after cataract surgery...
  65. doi Management of focal limbal stem cell deficiency associated with soft contact lens wear
    Bennie H Jeng
    Cole Eye Institute, Cleveland Clinic, Cleveland, OH, USA
    Cornea 30:18-23. 2011
    ..To present the varying manifestations of and the treatment methods for corneal epitheliopathies because of focal limbal stem cell deficiency (LSCD) associated with soft contact lens (SCL) wear...
  66. pmc From hair to cornea: toward the therapeutic use of hair follicle-derived stem cells in the treatment of limbal stem cell deficiency
    Ewa Anna Meyer-Blazejewska
    Department of Ophthalmology, University of Erlangen Nurnberg, Erlangen, Germany
    Stem Cells 29:57-66. 2011
    ..These data highlight the therapeutic properties of using HFSC to treat LSCD in a mouse model while demonstrating a strong translational potential and points to the niche as a key factor for determining stem cell differentiation...
  67. doi Location of corneal epithelial stem cells
    Tung Tien Sun
    Department of Cell Biology, New York University School of Medicine, New York, New York 10016, USA
    Nature 463:E10-1; discussion E11. 2010
    ..We point out here that these suggestions are inconsistent with many known growth, differentiation and cell migration properties of the anterior ocular epithelia...
  68. ncbi Transcriptional profiling of enriched populations of stem cells versus transient amplifying cells. A comparison of limbal and corneal epithelial basal cells
    Mingyuan Zhou
    Department of Dermatology, Feinberg School of Medicine, Chicago, Illinois 60611, USA
    J Biol Chem 281:19600-9. 2006
    ..Our findings indicate that we can obtain accurate gene expression profiles of the stem cell-enriched limbal basal cell population in their "natural" quiescent state...
  69. doi Differentially expressed genes associated with human limbal epithelial phenotypes: new molecules that potentially facilitate selection of stem cell-enriched populations
    Lili Takacs
    Department of Ophthalmology, Medical and Health Science Center, University of Debrecen, Debrecen, Hungary
    Invest Ophthalmol Vis Sci 52:1252-60. 2011
    ..The aim of this study was to identify differentially expressed genes in the human limbal epithelium by microarray analysis...
  70. ncbi N-Cadherin is expressed by putative stem/progenitor cells and melanocytes in the human limbal epithelial stem cell niche
    Ryuhei Hayashi
    Department of Ophthalmology, Tohoku University School of Medicine, 1 1 Seiryomachi, Aoba ku, Sendai 980 8574, Japan
    Stem Cells 25:289-96. 2007
    ..These results indicate that N-cadherin may be a critical cell-to-cell adhesion molecule between corneal epithelial stem/progenitor cells and their corresponding niche cells in the limbal epithelium...
  71. pmc Morphological characteristics of the limbal epithelial crypt
    Vijay A Shanmuganathan
    The Larry A Donoso Laboratory for Eye Research, Division of Ophthalmology and Visual Sciences, University of Nottingham, United Kingdom
    Br J Ophthalmol 91:514-9. 2007
    ..The purpose of this study was to further evaluate the distribution, immunophenotypical, and ultra structural characteristics of the LEC as a putative niche of stem cells...
  72. pmc Nerve growth factor and its receptor TrkA serve as potential markers for human corneal epithelial progenitor cells
    Hong Qi
    Ocular Surface Center, Cullen Eye Institute, Department of Ophthalmology, Baylor College of Medicine, 6565 Fannin Street, NC 205, Houston, TX 77030, USA
    Exp Eye Res 86:34-40. 2008
    ..The spatial expression of NGF and TrkA by small clusters of basal cells interspersed between negative cell patches suggests that they are potential markers for human corneal epithelial progenitor cells...
  73. pmc Phenotypic characterization of human corneal epithelial cells expanded ex vivo from limbal explant and single cell cultures
    Hyun Seung Kim
    Ocular Surface Center, Cullen Eye Institute, Department of Ophthalmology, Baylor College of Medicine, 6565 Fannin Street, NC 205, Houston, TX 77030, USA
    Exp Eye Res 79:41-9. 2004
    ..Slow-cycling BrdU-label retaining cells, that are characteristic of stem cells, were identified in the cultures...
  74. ncbi Different cell sizes in human limbal and central corneal basal epithelia measured by confocal microscopy and flow cytometry
    Andre C Romano
    TissueTech, Inc, and Ocular Surface Research and Education Foundation, 7000 SW 97 Avenue, Miami, Florida 33173, USA
    Invest Ophthalmol Vis Sci 44:5125-9. 2003
    ..The current study was conducted to determine whether this spatial SC arrangement is reflected in differences in the cell size between limbal and corneal cells...
  75. ncbi Stem cell markers in the human posterior limbus and corneal endothelium of unwounded and wounded corneas
    Sara L McGowan
    Department of Vision Sciences, Vision Science Research Center, School of Optometry, The University of Alabama at Birmingham, Birmingham, AL 35294, USA
    Mol Vis 13:1984-2000. 2007
    ..This study was initiated to demonstrate the presence of such stem cells in the region of the posterior limbus and to show the response of these cells to corneal wounding...
  76. pmc Mesenchymal cells from limbal stroma of human eye
    Naresh Polisetty
    Sudhakar Sreekanth Ravi Stem Cell Biology Laboratory, LV Prasad Eye Institute, Kallam Anji Reddy Campus, Hyderabad, India
    Mol Vis 14:431-42. 2008
    ..In this study, we aimed to report the presence of mesenchymal cells resembling bone marrow-derived cells (MSC-BM) in the limbus of the human eye...
  77. pmc C/EBPdelta regulates cell cycle and self-renewal of human limbal stem cells
    Vanessa Barbaro
    Epithelial Stem Cell Research Center, The Veneto Eye Bank Foundation, H SS Giovanni and Paolo, 30100 Venice, Italy
    J Cell Biol 177:1037-49. 2007
    ....
  78. ncbi The role of ultrasound biomicroscopy in surgical planning for limbal dermoids
    I M Lanzl
    Glaucoma Service, Wills Eye Hospital, Jefferson Medical College, Thomas Jefferson University, Philadelphia, Pennsylvania, USA
    Cornea 17:604-6. 1998
    ..Depth information is difficult to obtain by slit-lamp examination because the structure is opaque. We investigated the use of ultrasound biomicroscopic (UBM) examination for surgical planning in limbal dermoids...
  79. pmc Isoforms of DeltaNp63 and the migration of ocular limbal cells in human corneal regeneration
    Enzo Di Iorio
    Epithelial Stem Cell Research Center, Veneto Eye Bank Foundation, 30122 Venice, Italy
    Proc Natl Acad Sci U S A 102:9523-8. 2005
    ..The beta and gamma isoforms, being suprabasal and virtually absent from the resting limbus, are not stem cell markers but are likely to play a role in epithelial differentiation specifically during the process of corneal regeneration...
  80. pmc Spontaneous corneal hem- and lymphangiogenesis in mice with destrin-mutation depend on VEGFR3 signaling
    Claus Cursiefen
    Department of Ophthalmology, Friedrich Alexander University Erlangen Nurnberg, Schwabachanlage 6, 91054 Erlangen, Germany
    Am J Pathol 166:1367-77. 2005
    ....
  81. doi Challenges to the study of asymmetric cell division in corneal and limbal epithelia
    Federico Castro-Muñozledo
    Department of Cell Biology, Centro de Investigacion y de Estudios Avanzados del IPN, Apdo Postal 14 740, Mexico City 07000, Mexico
    Exp Eye Res 92:4-9. 2011
    ..Future studies should be oriented to understand the regulation of the asymmetrical/symmetrical division, and the control of cell fate by the niche...
  82. ncbi Human limbal progenitor cell characteristics are maintained in tissue culture
    Shaohui Liu
    Singapore Eye Research Institute, Singapore
    Ann Acad Med Singapore 35:80-6. 2006
    ..To determine the differentiation of human limbal epithelial cells in tissue culture...
  83. ncbi Treatment of superior limbic keratoconjunctivitis by application of autologous serum
    E Goto
    Department of Ophthalmology, Tokyo Dental College, Ichikawa City, Chiba, Japan
    Cornea 20:807-10. 2001
    ..The average rose bengal and fluorescein score improved ( p < 0.05), and there was subjective improvement. CONCLUSION: Autologous serum application can be used as an alternative mode of therapy in SLK...
  84. doi Culture of corneal limbal epithelial stem cells: experience from benchtop to bedside in a tertiary care hospital in India
    Shweta Sharma
    Department of Ophthalmology, Dr Rajendra Prasad Centre for Ophthalmic Sciences, All India Institute of Medical Sciences, New Delhi, India
    Cornea 30:1223-32. 2011
    ..To standardize the technique of ex vivo culture of limbal epithelial stem cells (LESCs) using locally available adapted methods and evaluate the outcome of transplantation in patients with limbal stem cell deficiency...
  85. doi Differences in the protein expression in limbal versus central human corneal epithelium--a search for stem cell markers
    Mikkel Lyngholm
    Department of Ophthalmology, Aarhus University Hospital, Norrebrogade 44, 8000 Aarhus C, Denmark
    Exp Eye Res 87:96-105. 2008
    ..SOD2 and CK15 identify clusters of limbal basal cells and therefore they may serve as markers for limbal stem cells in conjunction with the earliest transient amplifying cells...
  86. pmc Distribution of label-retaining cells in the limbal epithelium of a mouse eye
    Jin Zhao
    Department of Ophthalmology, Columbia University, New York, NY, USA
    J Histochem Cytochem 57:177-85. 2009
    ..These results show that LRCs are present asymmetrically in a narrow zone within the limbus that can be defined precisely in reference to a newly defined anatomical boundary line between the cornea and the limbus...
  87. doi Ocular regeneration by stem cells: present status and future prospects
    G Astrid Limb
    UCL Institute of Ophthalmology, 11 Bath Street, London EC1V 9EL, UK
    Br Med Bull 85:47-61. 2008
    ..Here, we discuss the current status and limitations on the application of stem cells to ocular therapies, and consider the future prospects for their use in the restoration of vision...
  88. doi Ex vivo construction of an artificial ocular surface by combination of corneal limbal epithelial cells and a compressed collagen scaffold containing keratocytes
    Shengli Mi
    School of Pharmacy, University of Reading, Reading, UK
    Tissue Eng Part A 16:2091-100. 2010
    ....
  89. ncbi Comparison of characteristics of cultured limbal cells on denuded amniotic membrane and fresh conjunctival, limbal and corneal tissues
    Hossein Baharvand
    Department of Stem Cells, Royan Institute, PO Box 19395 4644, Tehran, Iran
    Dev Growth Differ 49:241-51. 2007
    ..Also, the expression of traditional SC markers may not be a reliable indicator of limbal SC and there is an increasing need to determine factor(s) involved in their stemness...
  90. doi Loss of corneal epithelial stem cell properties in outgrowths from human limbal explants cultured on intact amniotic membrane
    Sai Kolli
    North East Stem Cell Institute, Newcastle University, Central Parkway, Newcastle upon Tyne, NE1 3BZ, UK
    Regen Med 3:329-42. 2008
    ..In particular, the expression of putative stem cell markers and the colony-forming efficiency of the different zones of the outgrowth were studied...
  91. pmc Patterned expression of neurotrophic factors and receptors in human limbal and corneal regions
    Hong Qi
    Ocular Surface Center, Cullen Eye Institute, Department of Ophthalmology, Baylor College of Medicine, Houston, TX 77030, USA
    Mol Vis 13:1934-41. 2007
    ..To evaluate the expression patterns of neurotrophic factors (NTFs) and their receptors in the human cornea with the intention of exploring the role of NTFs in maintaining corneal epithelial stem cells in the limbus...
  92. doi Localization of the low-affinity nerve growth factor receptor p75 in human limbal epithelial cells
    Nick Di Girolamo
    Inflammatory Diseases Research Unit, School of Medical Sciences, University of New South Wales, and Department of Ophthalmology, Prince of Wales Hospital, Sydney, NSW, Australia
    J Cell Mol Med 12:2799-811. 2008
    ..p75 may represent an additional ocular surface epithelial stem/progenitor cell signature gene...
  93. pmc Autologous transplantation of conjunctival epithelial cells cultured on amniotic membrane in a rabbit model
    Kyoko Ono
    Department of Ophthalmology, University of Tokyo Graduate School of Medicine, Tokyo, Japan
    Mol Vis 13:1138-43. 2007
    ..To evaluate the feasibility of autologous transplantation in a rabbit model of conjunctival epithelial cells cultured on amniotic membrane for ocular surface reconstruction...
  94. ncbi Cytokeratin 15 can be used to identify the limbal phenotype in normal and diseased ocular surfaces
    Satoru Yoshida
    Cornea Center and Department of Ophthalmology, Tokyo Dental College, Chiba, Japan
    Invest Ophthalmol Vis Sci 47:4780-6. 2006
    ..To elucidate the expression pattern of K15, K19, K14, and K12 in human and mouse ocular surface epithelium as putative markers of epithelial phenotype...
  95. ncbi Amniotic membrane transplantation with or without limbal allografts for corneal surface reconstruction in patients with limbal stem cell deficiency
    S C Tseng
    Ocular Surface and Tear Center, Department of Ophthalmology, Bascom Palmer Eye Institute, Miami, Fla 33136, USA
    Arch Ophthalmol 116:431-41. 1998
    ..To examine whether amniotic membrane transplantation (AMT), in preparing the perilimbal stroma, enhances the success of allograft limbal transplantation (ALT)...
  96. ncbi Identification and characterization of limbal stem cells
    Ursula Schlötzer-Schrehardt
    Department of Ophthalmology, University Erlangen Nurnberg, Erlangen, Germany
    Exp Eye Res 81:247-64. 2005
    ....
  97. ncbi Factors affecting outcome following transplantation of ex vivo expanded limbal epithelium on amniotic membrane for total limbal deficiency in rabbits
    Seng Ei Ti
    Department of Ophthalmology, Bascom Palmer Eye Institute, University of Miami School of Medicine, Miami, Florida, USA
    Invest Ophthalmol Vis Sci 43:2584-92. 2002
    ....
  98. ncbi Human corneal stem cells display functional neuronal properties
    Gail M Seigel
    Department of Ophthalmology, University at Buffalo, Buffalo, NY 14214, USA
    Mol Vis 9:159-63. 2003
    ..In this study, we tested the hypothesis that human corneal stem cells, derived from neural ectoderm, can exhibit functional neuronal properties...
  99. ncbi Regional distribution of alpha9beta1 integrin within the limbus of the mouse ocular surface
    Ahdeah Pajoohesh-Ganji
    Department of Anatomy and Cell Biology, The George Washington University Medical Center, Washington, DC, USA
    Dev Dyn 230:518-28. 2004
    ..These studies are an important step toward improving our understanding of the development and cell biology of limbal basal cells...
  100. ncbi Phenotypic analyses of limbal epithelial cell cultures derived from donor corneoscleral rims
    Z Barnard
    School of Biomolecular and Biomedical Science, Griffith University, Brisbane, Queensland, Australia
    Clin Experiment Ophthalmol 29:138-42. 2001
    ..These findings demonstrate the potential importance of keratins 14 and 19 as markers of epithelial cell differentiation in the human cornea...
  101. doi Targeted cornea limbal stem/progenitor cell transfection in an organ culture model
    Bojun Zhao
    Department of Biological Sciences, Biomedical Sciences, Lancaster University, Lancaster, United Kingdom
    Invest Ophthalmol Vis Sci 49:3395-401. 2008
    ..To optimize a nonviral gene transfection system targeting the corneal limbal stem/progenitor cells...

Research Grants68

  1. Isolation of Limbal Epithelial Stem Cells
    ROBERT LAVKER; Fiscal Year: 2003
    ....
  2. SERINE PROTEINASE INHIBITORS IN OCULAR SURFACE EPITHELIA
    ROBERT LAVKER; Fiscal Year: 2003
    ..These studies will serve as a baseline for future studies on the therapeutic use of PAI-2 in ophthalmology. ..
  3. CORNEAL EPITHELIAL STEM CELLS
    ROBERT LAVKER; Fiscal Year: 2004
    ..g. persistent corneal epithelial breakdown), as well as neoplastic transformations giving rise to the unusually aggressive mucoepidermoid and sebaceous carcinomas. ..
  4. CORNEAL EPITHELIAL STEM CELLS
    ROBERT LAVKER; Fiscal Year: 2006
    ..g., persistent corneal epithelial breakdown, as well as neoplastic transformations giving rise ocular epithelial tumors). ..
  5. CORNEAL EPITHELIAL STEM CELLS
    ROBERT LAVKER; Fiscal Year: 2009
    ..g., persistent corneal epithelial breakdown, as well as neoplastic transformations giving rise ocular epithelial tumors). ..
  6. OCULAR SURFACE EPITHELIAL PRECURSORS
    JOSE WOLOSIN; Fiscal Year: 2007
    ..Completion of these initial studies will provide critical new information on the features of the ocular precursor cell pool. ..
  7. Gene Gun Technology, Opioids, and Corneal Diseases
    IAN ZAGON; Fiscal Year: 2003
    ..Ultimately, such data can be employed to design molecular strategies to remedy visual dysfunction. ..
  8. 4th International Research Workshop on Alopecia Areata
    ROBERT LAVKER; Fiscal Year: 2002
    ..Integration of the latest research findings with new AA therapies on the horizon will sum up the formal presentations. The evening session will be a poster discussion session in which new work will be presented. ..
  9. CELL MEMBRANE DIFFERENTIATION IN CORNEAL EPITHELIUM
    JOSE WOLOSIN; Fiscal Year: 2002
    ..abstract_text> ..
  10. OCULAR SURFACE EPITHELIAL PRECURSORS
    JOSE WOLOSIN; Fiscal Year: 2009
    ..Completion of these initial studies will provide critical new information on the features of the ocular precursor cell pool. ..
  11. ADHESION MOLECULES AND EPIDERMAL STRATIFICATION
    ROBERT LAVKER; Fiscal Year: 2001
    ....
  12. OCULAR SURFACE STEM CELL GENES
    JOSE WOLOSIN; Fiscal Year: 2006
    ..Given our current very limited understanding of stem cells, acquisition of a gene library is an essential, high impact step in the long path to unravel the process underlying the functionality of these privileged cells. ..
  13. Naltrexone as a Novel Treatment for Diabetic Keratopathy
    IAN ZAGON; Fiscal Year: 2007
    ..The proposed use of biotherapy with NTX is an innovative approach whereby the patient's own growth regulatory mechanisms are manipulated to correct complications from diabetes. ..
  14. MicroRNAs and Corneal Epithelial Stem Cells
    ROBERT LAVKER; Fiscal Year: 2007
    ..Since miRNAs can function to prevent cell division, their identification and potential pharmacologic downregulation could be useful in promoting corneal epithelial wound repair. ..
  15. The Role of MicroRNAs in Corneal Epithelial Homeostasis
    Robert M Lavker; Fiscal Year: 2010
    ....
  16. CORNEAL EPITHELIAL STEM CELLS
    ROBERT LAVKER; Fiscal Year: 2009
    ..g., persistent corneal epithelial breakdown, as well as neoplastic transformations giving rise ocular epithelial tumors). ..
  17. CORNEAL EPITHELIAL STEM CELLS
    Robert M Lavker; Fiscal Year: 2010
    ..g., persistent corneal epithelial breakdown, as well as neoplastic transformations giving rise ocular epithelial tumors). ..
  18. CORNEAL EPITHELIAL STEM CELLS
    ROBERT LAVKER; Fiscal Year: 2000
    ..These studies should ultimately lead to a better understanding and therapy of ocular surface disorders including persistent corneal epithelial defect and various dry-eye conditions. ..
  19. CORNEAL EPITHELIAL STEM CELLS
    ROBERT LAVKER; Fiscal Year: 1993
    ..This will ultimately increase our knowledge on diseases of the ocular surface epithelia such as persistent corneal epithelial defect and the various dry- eye conditions...
  20. CORNEAL EPITHELIAL STEM CELLS
    ROBERT LAVKER; Fiscal Year: 1990
    ..This will ultimately increase our knowledge on the structure and function of corneal and limbal epithelia in man...
  21. Notch Signaling in the Corneal Epithelium
    Ali Djalilian; Fiscal Year: 2007
    ..These results will lead to the development of new treatments for patients with disabling visual conditions involving the surface of the eye. ..
  22. NICHE REGULATION OF LIMBAL STEM CELLS
    Scheffer C G Tseng; Fiscal Year: 2010
    ....
  23. NICHE REGULATION OF LIMBAL STEM CELLS
    Scheffer Tseng; Fiscal Year: 2007
    ....
  24. GROWTH AND DIFFERNTIATION OF OCULAR SURFACE EPITHELIA
    Scheffer Tseng; Fiscal Year: 1993
    ..Using the resistance of the SC but not the TAC to tumor-promoter (TPA) treatment, the two cells lines will be differentiated with respect to the mitogenic pathway mediated by protein kinase C...
  25. NEUROTROPHIC FACTOR MODULATION OF CORNEAL ENDOTHELIUM
    Shay Whey Koh; Fiscal Year: 2009
    ..abstract_text> ..
  26. Gene Delivery to the Trabecular Meshwork
    Douglas Rhee; Fiscal Year: 2004
    ..These studies would set the foundation for future evaluations of the function of MIF in the TM and MIF?s potential as a candidate for gene therapy for the treatment of glaucoma. ..
  27. O-Glycans on Mucins at the Ocular Surface
    PABLO ARGUESO; Fiscal Year: 2007
    ..aeruginosa to the O-linked carbohydrates of individual mucins collected from tears. B. Determine in vitro how inhibition of O-glycan synthesis on membrane-associated mucins affects P. aeruginosa adherence to corneal cells. ..