myofibrils

Summary

Summary: Highly organized bundles of actin (=ACTINS), MYOSINS, and other proteins in the cytoplasm of skeletal and cardiac muscle cells that contract by a sliding filament mechanism.

Top Publications

  1. pmc The UNC-45 chaperone is critical for establishing myosin-based myofibrillar organization and cardiac contractility in the Drosophila heart model
    Girish C Melkani
    Department of Biology, Molecular Biology Institute and Heart Institute, San Diego State University, San Diego, California, United States of America
    PLoS ONE 6:e22579. 2011
  2. ncbi Protein degradation by the ubiquitin-proteasome pathway in normal and disease states
    Stewart H Lecker
    Nephrology Division, Beth Isreal Deaconess, Harvard Medical School, Boston, Massachusetts, USA
    J Am Soc Nephrol 17:1807-19. 2006
  3. pmc Assembly and dynamics of myofibrils
    Joseph W Sanger
    Department of Cell and Developmental Biology, SUNY Upstate Medical University, Syracuse, NY 13210, USA
    J Biomed Biotechnol 2010:858606. 2010
  4. ncbi Transient association of titin and myosin with microtubules in nascent myofibrils directed by the MURF2 RING-finger protein
    Véronique Pizon
    European Molecular Biology Laboratory, Cell Biology Division, Heidelberg, Germany
    J Cell Sci 115:4469-82. 2002
  5. pmc The organization of titin filaments in the half-sarcomere revealed by monoclonal antibodies in immunoelectron microscopy: a map of ten nonrepetitive epitopes starting at the Z line extends close to the M line
    D O Fürst
    Max Planck Institute for Biophysical Chemistry, Gottingen, Federal Republic of Germany
    J Cell Biol 106:1563-72. 1988
  6. ncbi Chaperone-mediated folding and assembly of myosin in striated muscle
    Rajani Srikakulam
    Department of Pathology and Laboratory Medicine, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Piscataway, NJ 08854, USA
    J Cell Sci 117:641-52. 2004
  7. pmc Modulation of energy transfer pathways between mitochondria and myofibrils by changes in performance of perfused heart
    Marko Vendelin
    Laboratory of Systems Biology, Institute of Cybernetics, Tallinn University of Technology, Akadeemia 21, 12618 Tallinn, Estonia
    J Biol Chem 285:37240-50. 2010
  8. ncbi Nebulin and N-WASP cooperate to cause IGF-1-induced sarcomeric actin filament formation
    Kazunori Takano
    Department of Biology, Graduate School of Science, Chiba University, 1 33 Yayoicho, Inageku, Chiba 263 8522, Japan
    Science 330:1536-40. 2010
  9. pmc Muscle-specific RING finger-1 interacts with titin to regulate sarcomeric M-line and thick filament structure and may have nuclear functions via its interaction with glucocorticoid modulatory element binding protein-1
    Abigail S McElhinny
    Department of Cell Biology and Anatomy, University of Arizona, Tucson, AZ 85724, USA
    J Cell Biol 157:125-36. 2002
  10. pmc Myofibrillar myopathies
    Duygu Selcen
    Department of Neurology, Division of Child Neurology and Neuromuscular Disease Research Laboratory, Mayo Clinic College of Medicine, Mayo Clinic, Rochester, MN, USA
    Neuromuscul Disord 21:161-71. 2011

Detail Information

Publications252 found, 100 shown here

  1. pmc The UNC-45 chaperone is critical for establishing myosin-based myofibrillar organization and cardiac contractility in the Drosophila heart model
    Girish C Melkani
    Department of Biology, Molecular Biology Institute and Heart Institute, San Diego State University, San Diego, California, United States of America
    PLoS ONE 6:e22579. 2011
    ..Structural analysis showed reduced myofibrils, myofibrillar disarray, and greatly decreased cardiac myosin accumulation...
  2. ncbi Protein degradation by the ubiquitin-proteasome pathway in normal and disease states
    Stewart H Lecker
    Nephrology Division, Beth Isreal Deaconess, Harvard Medical School, Boston, Massachusetts, USA
    J Am Soc Nephrol 17:1807-19. 2006
  3. pmc Assembly and dynamics of myofibrils
    Joseph W Sanger
    Department of Cell and Developmental Biology, SUNY Upstate Medical University, Syracuse, NY 13210, USA
    J Biomed Biotechnol 2010:858606. 2010
    We review some of the problems in determining how myofibrils may be assembled and just as importantly how this contractile structure may be renewed by sarcomeric proteins moving between the sarcomere and the cytoplasm...
  4. ncbi Transient association of titin and myosin with microtubules in nascent myofibrils directed by the MURF2 RING-finger protein
    Véronique Pizon
    European Molecular Biology Laboratory, Cell Biology Division, Heidelberg, Germany
    J Cell Sci 115:4469-82. 2002
    ..microtubules associate transiently with sarcomeric myosin and later with A-band titin when non-striated myofibrils differentiate into mature sarcomeres...
  5. pmc The organization of titin filaments in the half-sarcomere revealed by monoclonal antibodies in immunoelectron microscopy: a map of ten nonrepetitive epitopes starting at the Z line extends close to the M line
    D O Fürst
    Max Planck Institute for Biophysical Chemistry, Gottingen, Federal Republic of Germany
    J Cell Biol 106:1563-72. 1988
    ..We discuss the results in relation to titin molecules having half-sarcomere lengths. The three nebulin antibodies so far characterized again give rise to distinct pairs of stripes. These locate close to the N2 line...
  6. ncbi Chaperone-mediated folding and assembly of myosin in striated muscle
    Rajani Srikakulam
    Department of Pathology and Laboratory Medicine, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Piscataway, NJ 08854, USA
    J Cell Sci 117:641-52. 2004
    ..in bright globular foci and short filamentous structures that are later replaced by brightly fluorescent myofibrils. Time-lapse microscopy shows that the intermediates are dynamic and are present in elongating and fusing ..
  7. pmc Modulation of energy transfer pathways between mitochondria and myofibrils by changes in performance of perfused heart
    Marko Vendelin
    Laboratory of Systems Biology, Institute of Cybernetics, Tallinn University of Technology, Akadeemia 21, 12618 Tallinn, Estonia
    J Biol Chem 285:37240-50. 2010
    In the heart, the energy supplied by mitochondria to myofibrils is continuously and finely tuned to the contraction requirement over a wide range of cardiac loads...
  8. ncbi Nebulin and N-WASP cooperate to cause IGF-1-induced sarcomeric actin filament formation
    Kazunori Takano
    Department of Biology, Graduate School of Science, Chiba University, 1 33 Yayoicho, Inageku, Chiba 263 8522, Japan
    Science 330:1536-40. 2010
    ..phosphatidylinositol 3-kinase-Akt signaling formed a complex of nebulin and N-WASP at the Z bands of myofibrils by interfering with glycogen synthase kinase-3β in mice...
  9. pmc Muscle-specific RING finger-1 interacts with titin to regulate sarcomeric M-line and thick filament structure and may have nuclear functions via its interaction with glucocorticoid modulatory element binding protein-1
    Abigail S McElhinny
    Department of Cell Biology and Anatomy, University of Arizona, Tucson, AZ 85724, USA
    J Cell Biol 157:125-36. 2002
    ..The dual interactions of MURF-1 with titin and GMEB-1 may link myofibril signaling pathways (perhaps including titin's kinase domain) with muscle gene expression...
  10. pmc Myofibrillar myopathies
    Duygu Selcen
    Department of Neurology, Division of Child Neurology and Neuromuscular Disease Research Laboratory, Mayo Clinic College of Medicine, Mayo Clinic, Rochester, MN, USA
    Neuromuscul Disord 21:161-71. 2011
    ..However, in the majority of the myofibrillar myopathy patients the disease gene awaits discovery...
  11. pmc Muscle giants: molecular scaffolds in sarcomerogenesis
    Aikaterini Kontrogianni-Konstantopoulos
    Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    Physiol Rev 89:1217-67. 2009
    ....
  12. pmc Self-organization of muscle cell structure and function
    Anna Grosberg
    Disease Biophysics Group, Wyss Institute for Biologically Inspired Engineering, School of Engineering and Applied Sciences, Harvard University, Cambridge, Massachusetts, United States of America
    PLoS Comput Biol 7:e1001088. 2011
    ..fibers, premyofibrils and focal adhesions, as well as cooperative alignment and parallel bundling of nascent myofibrils. Our results suggest that a hierarchy of mechanisms regulate the self-organization of the contractile ..
  13. ncbi Homogeneity of cardiac contraction despite physiological asynchrony of depolarization: a model study
    R C P Kerckhoffs
    Department of Biomedical Engineering, Eindhoven University of Technology, Whoog 4 105, P O Box 513, 5600 Eindhoven, The Netherlands
    Ann Biomed Eng 31:536-47. 2003
    ..The present finding may indicate that electromechanical delay times are heterogeneously distributed, such that a contraction in a normal heart is more synchronous than depolarization...
  14. pmc History-dependent properties of skeletal muscle myofibrils contracting along the ascending limb of the force-length relationship
    Clara Pun
    Department of Kinesiology and Physical Education, Pine Avenue West 475, Montreal QC H2W1S4, Canada
    Proc Biol Sci 277:475-84. 2010
    ..Rabbit psoas myofibrils were isolated and attached between two microneedles for force measurements...
  15. ncbi Intercellular and extracellular mechanotransduction in cardiac myocytes
    J Yasha Kresh
    Department of Cardiothoracic Surgery, Drexel University College of Medicine, 245 North 15th Street, MS 111, Philadelphia, PA 19102, USA
    Pflugers Arch 462:75-87. 2011
    ....
  16. pmc Pathogenity of some limb girdle muscular dystrophy mutations can result from reduced anchorage to myofibrils and altered stability of calpain 3
    Natalia Ermolova
    Department of Neurology, David Geffen School of Medicine at University of California, Los Angeles, CA 90095, USA
    Hum Mol Genet 20:3331-45. 2011
    ..Our data suggest that R448H and D705G mutations affect both CAPN3s anchorage to titin and its stability. These studies reveal a novel mechanism by which mutations that spare enzymatic activity can still lead to calpainopathy...
  17. pmc SmyD1, a histone methyltransferase, is required for myofibril organization and muscle contraction in zebrafish embryos
    Xungang Tan
    Center of Marine Biotechnology, University of Maryland Biotechnology Institute, Baltimore, MD 21202, USA
    Proc Natl Acad Sci U S A 103:2713-8. 2006
    ..Together, these data indicate that SmyD1a and SmyD1b are histone methyltransferases and play a critical role in myofibril organization during myofiber maturation...
  18. ncbi Targeted homozygous deletion of M-band titin in cardiomyocytes prevents sarcomere formation
    Hanny Musa
    Institute for Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds, Leeds, LS2 9JT, UK
    J Cell Sci 119:4322-31. 2006
    ..These results show that integration of the M-band region of titin is required for myosin filament assembly, M-band formation and maturation of the Z-disk...
  19. pmc Knockdown and overexpression of Unc-45b result in defective myofibril organization in skeletal muscles of zebrafish embryos
    Elena P Bernick
    University of Maryland School of Medicine Interdisciplinary Training Program in Muscle Biology, Baltimore, MD 21201, USA
    BMC Cell Biol 11:70. 2010
    ..The effects of unc-45b knockdown on other sarcomeric structures and the phenotype of Unc-45b overexpression, however, are poorly understood in vertebrates...
  20. ncbi Specific interaction of the potassium channel beta-subunit minK with the sarcomeric protein T-cap suggests a T-tubule-myofibril linking system
    T Furukawa
    Department of Physiology, Akita University School of Medicine, Japan
    J Mol Biol 313:775-84. 2001
    ..Striated staining of minK was observed in non-washed, membrane-intact cardiac myofibrils, but not in well-washed, membrane-removed cardiac myofibrils, suggesting that minK localizes on T-tubular ..
  21. ncbi Myofibrillar myopathies: a clinical and myopathological guide
    Rolf Schroder
    Institute of Neuropathology and Department of Neurology, University Hospital Erlangen, Erlangen, Germany
    Brain Pathol 19:483-92. 2009
    ..This review focuses on the clinical and myopathological aspects of genetically defined MFMs, and shall provide a diagnostic guide for this numerically significant group of protein aggregate myopathies...
  22. ncbi Desmin and actin alterations in human muscles affected by delayed onset muscle soreness: a high resolution immunocytochemical study
    Ji Guo Yu
    Department of Integrative Medical Biology, Section for Anatomy, Umea University, 901 87 Umea, Sweden
    Histochem Cell Biol 118:171-9. 2002
    ..7% (SD 3.9%) and 11.4% (SD 4.6%), respectively. We suggest that the increased staining of actin and desmin reflects an increased synthesis of these proteins as part of an adaptation process following the unaccustomed eccentric exercise...
  23. ncbi Functional consequences of hypertrophic and dilated cardiomyopathy-causing mutations in alpha-tropomyosin
    Audrey N Chang
    Department of Medicine, Mayo Clinic College of Medicine, Mayo Clinic, Rochester, Minnesota 55905, USA
    J Biol Chem 280:34343-9. 2005
    ..The endogenous Tm-Tn complex was selectively removed from myofibrils and replaced with recombinant wild-type or mutant proteins...
  24. ncbi Genesis of muscle fiber-type diversity during mouse embryogenesis relies on Six1 and Six4 gene expression
    Anne Françoise Richard
    Institut Cochin, Universite Paris Descartes, Sorbonne Paris Cité, CNRS UMR 8104, Paris, France
    Dev Biol 359:303-20. 2011
    ..Six genes thus behave as essential global regulators of muscle gene expression, as well as a central switch to drive the skeletal muscle fast phenotype during fetal development...
  25. pmc Muscle wasting and interleukin-6-induced atrogin-I expression in the cachectic Apc ( Min/+ ) mouse
    Kristen A Baltgalvis
    Exercise Science Department, University of South Carolina, Columbia, USA
    Pflugers Arch 457:989-1001. 2009
    ..These data suggest that high circulating IL-6 levels induce type IIB fiber CSA loss in Apc ( Min/+ ) mice, and circulating IL-6 is sufficient to regulate Atrogin-I gene expression in cachectic mice...
  26. ncbi Interaction between PEVK-titin and actin filaments: origin of a viscous force component in cardiac myofibrils
    M Kulke
    Institute of Physiology, University of Heidelberg, Heidelberg, Germany
    Circ Res 89:874-81. 2001
    ..To probe the interaction in a more physiological environment, nonactivated cardiac myofibrils were stretched quickly, and force was measured during the subsequent hold period...
  27. ncbi AMPK activation stimulates myofibrillar protein degradation and expression of atrophy-related ubiquitin ligases by increasing FOXO transcription factors in C2C12 myotubes
    Kazuki Nakashima
    Molecular Nutrition Research Team, National Institute of Livestock and Grassland Science, Ikenoda, Tsukuba, Japan
    Biosci Biotechnol Biochem 71:1650-6. 2007
    ..These results indicate that activation of AMPK stimulates myofibrillar protein degradation through the expression of atrogin-1/MAFbx and MuRF1 by increasing FOXO transcription factors in skeletal muscles...
  28. pmc Thin filaments elongate from their pointed ends during myofibril assembly in Drosophila indirect flight muscle
    M Mardahl-Dumesnil
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    J Cell Biol 155:1043-53. 2001
    ..Although pointed-end capping by Tmod controls thin filament lengths in assembled myofibrils, its role in length specification during de novo myofibril assembly is not established...
  29. ncbi MURF-1 and MURF-2 target a specific subset of myofibrillar proteins redundantly: towards understanding MURF-dependent muscle ubiquitination
    Stephanie H Witt
    Institut fur Anasthesiologie und Operative Intensivmedizin, Universitatsklinikum Mannheim, Mannheim 68167, Germany
    J Mol Biol 350:713-22. 2005
    ..These data raise the possibility that MURF-1 may coordinately regulate the energy metabolism of mitochondrial and cytoplasmic compartments...
  30. pmc Diffusion spectrum MRI tractography reveals the presence of a complex network of residual myofibers in infarcted myocardium
    David E Sosnovik
    Martinos Center for Biomedical Imaging, Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Boston, Mass, USA
    Circ Cardiovasc Imaging 2:206-12. 2009
    ..Unlike diffusion tensor imaging, DSI tractography resolves multiple myofiber populations per voxel, thus generating accurate 3D tractograms, which we present in the myocardium for the first time...
  31. pmc Desmin is essential for the tensile strength and integrity of myofibrils but not for myogenic commitment, differentiation, and fusion of skeletal muscle
    Z Li
    Station Centrale de Microscopie Electronique, Institut Pasteur, Paris, France
    J Cell Biol 139:129-44. 1997
    ..The results presented here show that desmin is essential to maintain the structural integrity of highly solicited skeletal muscle...
  32. ncbi Focal adhesion kinase is essential for costamerogenesis in cultured skeletal muscle cells
    Navaline L Quach
    Department of Neurology and Neurological Sciences, Stanford University School of Medicine, Stanford, CA 94305, USA
    Dev Biol 293:38-52. 2006
    A central question in muscle biology is how costameres are formed and become aligned with underlying myofibrils in mature tissues...
  33. ncbi Exercise induces a cardiac mitochondrial phenotype that resists apoptotic stimuli
    Andreas N Kavazis
    Department of Applied Physiology and Kinesiology, University of Florida, Gainesville, FL 32611, USA
    Am J Physiol Heart Circ Physiol 294:H928-35. 2008
    ..Furthermore, these results are consistent with the concept that exercise-induced mitochondrial adaptations contribute to exercise-induced cardioprotection...
  34. pmc Diastolic dysfunction in familial hypertrophic cardiomyopathy transgenic model mice
    Theodore P Abraham
    Division of Cardiology, Johns Hopkins University, Baltimore, MD, USA
    Cardiovasc Res 82:84-92. 2009
    ....
  35. ncbi Protein kinase G modulates human myocardial passive stiffness by phosphorylation of the titin springs
    Martina Kruger
    Physiology and Biophysics Unit, University of Muenster, Germany
    Circ Res 104:87-94. 2009
    ..determine whether PKG can phosphorylate titin and affect titin-based stiffness in skinned myofibers and isolated myofibrils. PKG in the presence of 8-pCPT-cGMP (cGMP) phosphorylated the 2 main cardiac titin isoforms, N2BA and N2B, in ..
  36. ncbi Metabolic consequences of functional complexes of mitochondria, myofibrils and sarcoplasmic reticulum in muscle cells
    T Andrienko
    Laboratory of Fundamental and Applied Bioenergetics, INSERM E0221, Joseph Fourier University, Grenoble, France
    J Exp Biol 206:2059-72. 2003
    ..Inside these complexes, mitochondrial functional state is controlled by channeling of ADP, mostly via energy- and phosphoryl-transfer networks, and apparently depends on the state of sarcomere structures...
  37. pmc Binding of an ankyrin-1 isoform to obscurin suggests a molecular link between the sarcoplasmic reticulum and myofibrils in striated muscles
    Paola Bagnato
    Molecular Medicine Section, Department of Neuroscience, University of Siena, 53100 Siena, Italy
    J Cell Biol 160:245-53. 2003
    ..The mechanisms that mediate the structural organization of the sarcoplasmic reticulum with respect to the myofibrils are, however, not known. We report here that ank1...
  38. ncbi The mode of myofibril remodelling in human skeletal muscle affected by DOMS induced by eccentric contractions
    Ji Guo Yu
    Department of Integrative Medical Biology, Section for Anatomy, Umea University, Sweden
    Histochem Cell Biol 119:383-93. 2003
    ..These were preferentially seen in regions with increased numbers of sarcomeres in parallel myofibrils. We propose that these staining patterns represent different stages of sarcomere formation...
  39. ncbi Propofol decreases myofilament Ca2+ sensitivity via a protein kinase C-, nitric oxide synthase-dependent pathway in diabetic cardiomyocytes
    Peter J Wickley
    Kent State University, Kent, Ohio, USA
    Anesthesiology 104:978-87. 2006
    ....
  40. ncbi Immunocytochemical studies using a monoclonal antibody to bovine cardiac titin on intact and extracted myofibrils
    S M Wang
    J Muscle Res Cell Motil 6:293-312. 1985
    ..The antibody recognizes a common antigenic site in striated muscles of several species. In relaxed myofibrils, specific staining at the A-I junction resulted in a doublet of fluorescent bands within a sarcomere...
  41. ncbi Myocardial ischemia selectively depletes cardiolipin in rabbit heart subsarcolemmal mitochondria
    E J Lesnefsky
    Division of Cardiology, Case Western Reserve University, Cleveland, Ohio 44106, USA
    Am J Physiol Heart Circ Physiol 280:H2770-8. 2001
    ..In contrast, oxidation through cytochrome oxidase in interfibrillar mitochondria (IFM), located between the myofibrils, remains unaffected...
  42. pmc Nitroxyl increases force development in rat cardiac muscle
    Tieying Dai
    Department of Anesthesiology and Critical Care Medicine, The Johns Hopkins University School of Medicine, Tower 711, 600 N Wolfe Street, Baltimore, MD 21287, USA
    J Physiol 580:951-60. 2007
    ..This is likely to be due to modulation of myofilament proteins that harbour reactive thiolate groups that are targets of HNO...
  43. pmc The NH2 terminus of titin spans the Z-disc: its interaction with a novel 19-kD ligand (T-cap) is required for sarcomeric integrity
    C C Gregorio
    Departments of Cell Biology and Anatomy, and Molecular and Cellular Biology, University of Arizona, Tucson, Arizona 85724, USA
    J Cell Biol 143:1013-27. 1998
    ....
  44. pmc Engineered troponin C constructs correct disease-related cardiac myofilament calcium sensitivity
    Bin Liu
    Department of Physiology and Cell Biology, The Ohio State University, Columbus, Ohio 43210, USA
    J Biol Chem 287:20027-36. 2012
    ..Thus, the present study provides a novel and versatile therapeutic strategy to restore diseased cardiac muscle Ca(2+) sensitivity...
  45. pmc Ranolazine improves cardiac diastolic dysfunction through modulation of myofilament calcium sensitivity
    Joshua D Lovelock
    Section of Cardiology, University of Illinois at Chicago, 60612, USA
    Circ Res 110:841-50. 2012
    ..Oxidative stress has been shown to increase late inward sodium current (I(Na)), reducing the net cytosolic Ca(2+) efflux...
  46. ncbi Translocation of HSP27 and MKBP in ischemic heart
    K Yoshida
    Department of Legal Medicine, Yamaguchi University School of Medicine, Ube, Japan
    Cell Struct Funct 24:181-5. 1999
    ..Two dimensional gel electrophoresis showed that ischemia increases the hyperphosphorylated form of HSP27. These data suggest that HSP27 and MKBP may be involved in the Z line protection against postischemic reperfusion injury...
  47. ncbi Subcellular movement and expression of HSP27, alphaB-crystallin, and HSP70 after two bouts of eccentric exercise in humans
    G Paulsen
    Norwegian School of Sport Sciences, Department of Anatomy, University of Oslo, P O Box 4014 U S, N 0806 Oslo, Norway
    J Appl Physiol (1985) 107:570-82. 2009
    ..The large amount of HSP27, alphaB-crystallin, and HSP70 in the cytoskeletal/myofibrillar fraction after a repeated bout of exercise suggests a protective role as part of the repeated-bout effect...
  48. pmc 2D and 3D-organized cardiac cells shows differences in cellular morphology, adhesion junctions, presence of myofibrils and protein expression
    Carolina Pontes Soares
    Instituto de Ciencias Biomedicas, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil
    PLoS ONE 7:e38147. 2012
    ..cells, contraction ability, proliferation rate, presence of intercellular adhesion structures, organization of myofibrils, mitochondria morphology, endoplasmic reticulum contents, cytoskeletal filaments and extracellular matrix ..
  49. pmc Cross-bridge kinetics in myofibrils containing familial hypertrophic cardiomyopathy R58Q mutation in the regulatory light chain of myosin
    P Mettikolla
    Department of Molecular Biology and Immunology and Center for Commercialization of Fluorescence Technologies, University of North Texas, Health Science Center, 3500 Camp Bowie Blvd, Fort Worth, TX 76107, USA
    J Theor Biol 284:71-81. 2011
    ..We studied the effect of the FHC-linked R58Q-RLC mutation on the kinetics of transgenic (Tg)-R58Q cardiac myofibrils. Kinetics was determined from the rate of change of orientation of actin monomers during muscle contraction...
  50. ncbi Association of the chaperone alphaB-crystallin with titin in heart muscle
    Belinda Bullard
    European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    J Biol Chem 279:7917-24. 2004
    ..alphaB-crystallin in cardiac muscle binds to myofibrils under conditions of ischemia, and previous work has shown that the protein binds to titin in the I-band of ..
  51. ncbi Evidence for myofibril remodeling as opposed to myofibril damage in human muscles with DOMS: an ultrastructural and immunoelectron microscopic study
    Ji Guo Yu
    Department of Integrative Medical Biology, Section for Anatomy, Umea University, 901 87 Umea, Sweden
    Histochem Cell Biol 121:219-27. 2004
    ..Our results strongly support that the myofibrillar and cytoskeletal alterations, considered to be the hallmarks of DOMS, reflect an adaptive remodeling of the myofibrils.
  52. ncbi Tension generation and relaxation in single myofibrils from human atrial and ventricular myocardium
    Nicoletta Piroddi
    Centro Interuniversitario di Medicina Molecolare e Biofisica Applicata, Florence, Italy
    Pflugers Arch 454:63-73. 2007
    Fast solution switching techniques in single myofibrils offer the opportunity to dissect and directly examine the sarcomeric mechanisms responsible for force generation and relaxation...
  53. pmc Properties of easily releasable myofilaments: are they the first step in myofibrillar protein turnover?
    Girija Neti
    Muscle Biology Group, 626 Shantz, University of Arizona, Tucson, AZ 85721, USA
    Am J Physiol Cell Physiol 296:C1383-90. 2009
    ..It has been proposed that the calpains could remove the outer layer of filaments from myofibrils as a first step in myofibrillar protein turnover...
  54. pmc Paralysis and delayed Z-disc formation in the Xenopus tropicalis unc45b mutant dicky ticker
    Timothy J Geach
    Division of Developmental Biology, MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK
    BMC Dev Biol 10:75. 2010
    ..Chaperone proteins specific to sarcomeric myosins have been shown to be necessary in zebrafish and invertebrates for proper muscle assembly and function...
  55. ncbi Functional consequences of the mutations in human cardiac troponin I gene found in familial hypertrophic cardiomyopathy
    F Takahashi-Yanaga
    Department of Clinical Pharmacology, Graduate School of Medical Sciences, Kyushu University, Fukuoka 812 8582, Japan
    J Mol Cell Cardiol 33:2095-107. 2001
    ..Exchanging the human cardiac troponin into isolated cardiac myofibrils or skinned cardiac muscle fibers showed that the mutations R145G, R145Q, R162W, DeltaK183 and K206Q induced a ..
  56. ncbi The mechanical behavior of individual sarcomeres of myofibrils isolated from rabbit psoas muscle
    Ivan Pavlov
    Department of Kinesiology and Physical Education, McGill University, Montreal, Quebec, Canada, H2W1S4
    Am J Physiol Cell Physiol 297:C1211-9. 2009
    ..Single myofibrils from rabbit psoas were transferred into a temperature-controlled (22 degrees C or 15 degrees C) experimental ..
  57. pmc Pre-power stroke cross bridges contribute to force during stretch of skeletal muscle myofibrils
    Dilson E Rassier
    Department of Kinesiology and Physical Education Faculty of Education, McGill University, 475 Pine Avenue West, Montreal, Quebec, Canada
    Proc Biol Sci 275:2577-86. 2008
    ..b>Myofibrils were activated in sarcomere lengths (SLs) between 2.2 and 2...
  58. pmc Residual force enhancement in myofibrils and sarcomeres
    V Joumaa
    University of Calgary, 2500 University Drive NW, Calgary, Alberta, Canada T2N 1N4
    Proc Biol Sci 275:1411-9. 2008
    ..Here, we studied force enhancement for the first time in isolated myofibrils (n=18) that, owing to the strict in series arrangement, allowed for evaluation of this property in individual ..
  59. ncbi Insights into the kinetics of Ca2+-regulated contraction and relaxation from myofibril studies
    Robert Stehle
    Institute of Physiology, University of Cologne, Robert Koch Str 39, 50931 Cologne, Germany
    Pflugers Arch 458:337-57. 2009
    ..techniques that analyse the chemical and mechanical kinetics of small components of a muscle fibre, subcellular myofibrils isolated from skeletal and cardiac muscle...
  60. ncbi Structural basis for activation of the titin kinase domain during myofibrillogenesis
    O Mayans
    European Molecular Biology Laboratory, Hamburg Outstation, Germany
    Nature 395:863-9. 1998
    ..Titin kinase phosphorylates the muscle protein telethonin in early differentiating myocytes, indicating that this kinase may act in myofibrillogenesis...
  61. ncbi Myofibrillogenesis in the first cardiomyocytes formed from isolated quail precardiac mesoderm
    Aiping Du
    Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 6058, USA
    Dev Biol 257:382-94. 2003
    De novo assembly of myofibrils was investigated in explants of precardiac mesoderm from quail embryos to address a controversy about different models of myofibrillogenesis...
  62. ncbi Lys184 deletion in troponin I impairs relaxation kinetics and induces hypercontractility in murine cardiac myofibrils
    Bogdan Iorga
    Institute of Vegetative Physiology, University of Cologne, Robert Koch Strasse 39, Cologne, Germany
    Cardiovasc Res 77:676-86. 2008
    ....
  63. ncbi Distinct mechanisms for diastolic dysfunction in diabetes mellitus and chronic pressure-overload
    Ines Falcao-Pires
    Department of Physiology and Cardiothoracic Surgery, Cardiovascular R and D Unit, Faculty of Medicine, University of Porto, Alameda Professor Hernani Monteiro, 4200 319 Porto, Portugal
    Basic Res Cardiol 106:801-14. 2011
    ..The association of these damages accelerates the progression of diastolic heart failure progression in diabetic-banded animals...
  64. pmc Myofibrillogenesis in the developing zebrafish heart: A functional study of tnnt2
    Wei Huang
    Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine, Rochester, MN 55905, USA
    Dev Biol 331:237-49. 2009
    ..In summary, our data indicates that zebrafish are a valuable in vivo model for studying both myofibrillogenesis and sarcomere-based cardiac diseases...
  65. ncbi Form follows function: how muscle shape is regulated by work
    B Russell
    Department of Physiology and Biophysics, College of Medicine, University of Illinois at Chicago, Chicago, Illinois 60612, USA
    J Appl Physiol (1985) 88:1127-32. 2000
    ..of cell shape and the composition and aggregation of contractile proteins into filaments, the sarcomere, and myofibrils. We examine how mechanical activity regulates the turnover and exchange of contraction proteins...
  66. pmc Actin-titin interaction in cardiac myofibrils: probing a physiological role
    W A Linke
    Institute of Physiology II, University of Heidelberg, Germany
    Biophys J 73:905-19. 1997
    The high stiffness of relaxed cardiac myofibrils is explainable mainly by the expression of a short-length titin (connectin), the giant elastic protein of the vertebrate myofibrillar cytoskeleton...
  67. ncbi Why choose myofibrils to study muscle myosin ATPase?
    Corinne Lionne
    UMR 5121, CNRS Université Montpellier I, Institut de Biologie, 4 boulevard Henri IV CS89508, 34960 Montpellier 2, France
    J Muscle Res Cell Motil 24:139-48. 2003
    ..In the presence of calcium, myofibrillar ATPase is activated and myofibrils either shorten with no external load (native myofibrils) or contract isometrically (cross-linked myofibrils)...
  68. ncbi Evidence of myofibrillar protein oxidation induced by postischemic reperfusion in isolated rat hearts
    Marcella Canton
    Dipartimento di Chimica Biologica, Viale G Colombo, 3, 35121 Padova, Italy
    Am J Physiol Heart Circ Physiol 286:H870-7. 2004
    ..Furthermore, the inhibition of MP oxidation by MPG might underlie the protective efficacy of antioxidants...
  69. ncbi Genetics of the Drosophila flight muscle myofibril: a window into the biology of complex systems
    J O Vigoreaux
    Department of Biology, University of Vermont, Burlington, VT 05405 USA
    Bioessays 23:1047-63. 2001
    ....
  70. pmc I-band titin in cardiac muscle is a three-element molecular spring and is critical for maintaining thin filament structure
    W A Linke
    Physiologisches Institut II, Universitat Heidelberg, D 69120 Heidelberg, Germany
    J Cell Biol 146:631-44. 1999
    ..N2-B-titin emerges as a unique region critical for both reversible extensibility and structural maintenance of cardiac myofibrils.
  71. ncbi Comparison of the small heat shock proteins alphaB-crystallin, MKBP, HSP25, HSP20, and cvHSP in heart and skeletal muscle
    Nikola Golenhofen
    Institute of Anatomy and Cell Biology, University of Wurzburg, Koellikerstrasse 6, 97070, Wurzburg, Germany
    Histochem Cell Biol 122:415-25. 2004
    ..In ischemic heart and skeletal muscle these five sHSPs translocated from cytosol to the Z-/I-area of myofibrils. Myofibrillar binding of all sHSPs was very tight and resisted for the most part extraction with 1 M NaSCN or 1 ..
  72. ncbi Impact of disease mutations on the desmin filament assembly process
    Harald Bär
    Department of Cardiology, University of Heidelberg, D 69120 Heidelberg, Germany
    J Mol Biol 360:1031-42. 2006
    ....
  73. pmc A new 185,000-dalton skeletal muscle protein detected by monoclonal antibodies
    B K Grove
    J Cell Biol 98:518-24. 1984
    ..antibodies (mAbs) against a 165-kd fraction, we also obtained mAbs that bound to the M line of isolated myofibrils as detected by indirect immunofluorescence, but recognized a protein band of 185 kd in immunoblotting ..
  74. pmc Mechanical stress-strain sensors embedded in cardiac cytoskeleton: Z disk, titin, and associated structures
    Masahiko Hoshijima
    Department of Medicine, University of California, San Diego, La Jolla, CA 92093 0734, USA
    Am J Physiol Heart Circ Physiol 290:H1313-25. 2006
    ..This review summarizes current knowledge of this rapidly developing area with focus on how the heart adjusts physiological remodeling process to meet with mechanical demands and how this process fails in cardiac pathologies...
  75. ncbi Molecular diversity of myofibrillar proteins: gene regulation and functional significance
    S Schiaffino
    Department of Biomedical Sciences, University of Padova, Padua, Italy
    Physiol Rev 76:371-423. 1996
    ..Both myosin and troponin isoforms contribute to the differences in the resistance to fatigue of muscle fibers...
  76. ncbi The M-band: an elastic web that crosslinks thick filaments in the center of the sarcomere
    Irina Agarkova
    Institute of Cell Biology, Swiss Federal Institute of Technology, ETH Honggerberg, CH 8093 Zurich
    Trends Cell Biol 15:477-85. 2005
    ..We propose that the M-band is in fact a dynamic structure that monitors the stress appearing in the thick filament lattice during contraction and quickly reorganizes to meet new physiological requirements...
  77. ncbi Obscurin is required for the lateral alignment of striated myofibrils in zebrafish
    Maide O Raeker
    Department of Pediatrics and Communicable Diseases, University of Michigan, Ann Arbor, Michigan 48109, USA
    Dev Dyn 235:2018-29. 2006
    ..independent morpholino antisense oligonucleotides resulted in diminished numbers and marked disarray of skeletal myofibrils, impaired lateral alignment of adjacent myofibrils, disorganization of the sarcoplasmic reticulum, somite ..
  78. pmc Aberrant myofibril assembly in tropomodulin1 null mice leads to aborted heart development and embryonic lethality
    Kimberly L Fritz-Six
    Department of Cell Biology, The Scripps Research Institute, 10550 N Torrey Pines Road, La Jolla, CA 92037, USA
    J Cell Biol 163:1033-44. 2003
    ..5. Confocal microscopy of hearts of E8-8.5 Tmod1 null embryos revealed structures resembling nascent myofibrils with continuous F-actin staining and periodic dots of alpha-actinin, indicating that I-Z-I complexes assembled ..
  79. ncbi Glucose and palmitic acid induce degeneration of myofibrils and modulate apoptosis in rat adult cardiomyocytes
    D Dyntar
    Division of Endocrinology and Diabetes, University Hospital, Zurich, Switzerland
    Diabetes 50:2105-13. 2001
    ..C2-dihydroceramide enhanced apoptosis of cardiomyocytes by 50%, accompanied by detrimental effects on the myofibrils. The palmitic acid-induced effects were impaired by fumonisin B1, an inhibitor of ceramide synthase...
  80. pmc Myofibrillogenesis in skeletal muscle cells in zebrafish
    Joseph W Sanger
    Department of Cell and Developmental Biology, SUNY Upstate Medical University, Syracuse, New York, USA
    Cell Motil Cytoskeleton 66:556-66. 2009
    ..model of myofibrillogenesis, based on observations in cultured avian muscle cells, proposes that mature myofibrils are preceded by two intermediary structures: premyofibrils and nascent myofibrils...
  81. ncbi Myofibrillar myopathy: clinical, morphological and genetic studies in 63 patients
    Duygu Selcen
    Department of Neurology, Neuromuscular Research Laboratory, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA
    Brain 127:439-51. 2004
    ..Further advances in defining the molecular causes of MFM will probably come from linkage studies of informative kinships or from systematic search for mutations in proteins participating in the intricate network supporting the Z-disk...
  82. pmc Myosin transducer mutations differentially affect motor function, myofibril structure, and the performance of skeletal and cardiac muscles
    Anthony Cammarato
    Department of Biology and Heart Institute, San Diego State University, San Diego, CA 92182 4614, USA
    Mol Biol Cell 19:553-62. 2008
    ..Our data suggest Drosophila is a valuable system for identifying and modeling mutations analogous to those associated with specific human muscle disorders...
  83. ncbi Differential involvement of sarcomeric proteins in myofibrillar myopathies: a morphological and immunohistochemical study
    Kristl G Claeys
    Institut de Myologie, Groupe Hospitalier Pitie Salpetriere, Paris, France
    Acta Neuropathol 117:293-307. 2009
    ....
  84. pmc Half-sarcomere dynamics in myofibrils during activation and relaxation studied by tracking fluorescent markers
    Ivo A Telley
    Laboratory for Biomechanics, ETH Zurich Honggerberg, 8093 Zurich, Switzerland
    Biophys J 90:514-30. 2006
    To study the dynamics of individual half-sarcomeres in striated muscle contraction, myofibrils prepared from rabbit psoas muscle and left ventricles of guinea pig were immunostained with two conjugated antibody complexes consisting of a ..
  85. pmc Hsp27 associates with the titin filament system in heat-shocked zebrafish cardiomyocytes
    Nathan R Tucker
    School of Molecular Biosciences, Washington State University, Pullman, WA 99164, USA
    Exp Cell Res 315:3176-86. 2009
    ..Here, we examined the association of Hsp27 with myofibrils in adult zebrafish myocardium subjected to hyperthermia and mechanical stretching...
  86. ncbi Functional properties of the titin/connectin-associated proteins, the muscle-specific RING finger proteins (MURFs), in striated muscle
    Carol C Gregorio
    Department of Cell Biology and Anatomy, University of Arizona, Tucson, AZ 85724, USA
    J Muscle Res Cell Motil 26:389-400. 2005
    ..is dependent upon the proper alignment and coordinated activities of several cytoskeletal networks including myofibrils, microtubules, and intermediate filaments...
  87. pmc Unfolding of titin domains explains the viscoelastic behavior of skeletal myofibrils
    A Minajeva
    Institute of Physiology and Pathophysiology, University of Heidelberg, D-69120 Heidelberg, Germany
    Biophys J 80:1442-51. 2001
    ..We show that isolated psoas myofibrils respond to a stretch-hold protocol with a characteristic force decay that becomes more pronounced following ..
  88. ncbi Not just a plasma membrane protein: in cardiac muscle cells alpha-II spectrin also shows a close association with myofibrils
    Pauline M Bennett
    The Randall Centre for Molecular Mechanisms of Cell Function, New Hunt s House, King s College London, Guy s Campus, London SE1 1UL, UK
    J Muscle Res Cell Motil 25:119-26. 2004
    ..reveals statistically significant clustering of gold particles near the Z-disc, within and close to the edge of myofibrils. betaII-spectrin and ankyrin-R and G are both known to occupy this region...
  89. pmc Dynamic behaviour of half-sarcomeres during and after stretch in activated rabbit psoas myofibrils: sarcomere asymmetry but no 'sarcomere popping'
    I A Telley
    Laboratory for Biomechanics, ETH Zurich, ETH Honggerberg, HCI E 357 1, CH 8093 Zurich, Switzerland
    J Physiol 573:173-85. 2006
    ..changes of individual half-sarcomeres during and after stretch in actively contracting, single rabbit psoas myofibrils containing 10-30 sarcomeres...
  90. pmc Myofibrillar architecture in engineered cardiac myocytes
    Kevin Kit Parker
    Disease Biophysics Group, School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA kkparker seas harvard edu
    Circ Res 103:340-2. 2008
    ..These data suggest that physical constrainment of muscle cells by extracellular matrix may be an important regulator of myofibrillar organization...
  91. ncbi Sarcomere alignment is regulated by myocyte shape
    Mark Anthony Bray
    Disease Biophysics Group, School of Engineering and Applied Sciences, Harvard University, Cambridge, Massachusetts, USA
    Cell Motil Cytoskeleton 65:641-51. 2008
    ..Furthermore, geometric boundaries such as corners induce localized myofibrillar anisotropy that becomes global as the myocyte aspect ratio increases...
  92. ncbi How to build a myofibril
    Joseph W Sanger
    Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 6058, USA
    J Muscle Res Cell Motil 26:343-54. 2005
    ..The models hypothesize: (a) stress fiber-like structures as templates for the assembly of myofibrils, (b) assembly in which the actin filaments and Z-bands form subunits independently from A-band subunits, with ..
  93. pmc Titin: major myofibrillar components of striated muscle
    K Wang
    Proc Natl Acad Sci U S A 76:3698-702. 1979
    ..proteins, we purified a combination of two uncommonly large proteins, designated as titin, from chicken breast myofibrils. Chemical and immunological studies indicated that titin is distinct from myosin, actin, and filamin...
  94. ncbi ELISA for monitoring lipid oxidation in chicken myofibrils through quantification of hexanal-protein adducts
    Carolyn F Goodridge
    Department of Food Science and Human Nutrition, Michigan State University, East Lansing, Michigan 48824, USA
    J Agric Food Chem 51:7533-9. 2003
    ..Freeze-dried myofibrils with added methyl linoleate (0...
  95. ncbi A nonsense mutation within the act88F actin gene disrupts myofibril formation in Drosophila indirect flight muscles
    C C Karlik
    Cell 38:711-9. 1984
    ..semi-dominant allele was isolated by Mogami and Hotta (1981) and was shown to disrupt the organization of myofibrils in indirect flight muscles...
  96. pmc Altered patterns of cardiac intercellular junction distribution in hypertrophic cardiomyopathy
    R Sepp
    Second Department of Internal Medicine, A Szent Györgyi University of Medicine, Szeged, Hungary
    Heart 76:412-7. 1996
    ..To examine the distribution pattern of intercellular junctions (the mechanically coupling desmosomes and the electrically coupling gap junctions) in hypertrophic cardiomyopathy (HCM) hearts showing myofibre disarray...
  97. ncbi Fructose diet treatment in mice induces fundamental disturbance of cardiomyocyte Ca2+ handling and myofilament responsiveness
    Kimberley M Mellor
    Dept of Physiology, Univ of Melbourne, Parkville, Victoria, Australia 3010
    Am J Physiol Heart Circ Physiol 302:H964-72. 2012
    ..These findings demonstrate that fructose diet-associated myocardial insulin resistance induces profound disturbance of cardiomyocyte Ca(2+) handling and responsiveness in the absence of altered systemic loading conditions...
  98. pmc Measurement of thin filament lengths by distributed deconvolution analysis of fluorescence images
    Ryan Littlefield
    Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    Biophys J 82:2548-64. 2002
    ..fluorescence, we were able to determine the thin filament lengths of isolated chicken pectoralis major myofibrils with an accuracy and precision comparable to electron microscopy...
  99. ncbi De novo myofibrillogenesis in C2C12 cells: evidence for the independent assembly of M bands and Z disks
    Aikaterini Kontrogianni-Konstantopoulos
    Dept of Physiology, School of Medicine, Univ of Maryland at Baltimore, 685 W Baltimore St, Baltimore, MD 21201, USA
    Am J Physiol Cell Physiol 290:C626-37. 2006
    ....
  100. pmc Cardiac myofibrillogenesis inside intact embryonic hearts
    Aiping Du
    Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 6058, USA
    Dev Biol 318:236-46. 2008
    How proteins assemble into sarcomeric arrays to form myofibrils is controversial...
  101. pmc Nebulin-deficient mice exhibit shorter thin filament lengths and reduced contractile function in skeletal muscle
    Marie Louise Bang
    Department of Medicine, Veterans Affairs Medical Center, University of California, San Diego, La Jolla, CA 92093, USA
    J Cell Biol 173:905-16. 2006
    ..The functional importance of nebulin in skeletal muscle function was revealed by isometric contractility assays, which demonstrated a dramatic reduction in force production in nebulin-deficient skeletal muscle...

Research Grants64

  1. Chymase-Mediated MMP Activation in Ishemia Reperfusion Injury
    LOUIS JOSEPH DELL'ITALIA; Fiscal Year: 2013
    ..Recent studies demonstrated activated MMP-2 within the cardiomyocyte that cleaved myofibrils (4-6) and nuclear matrix protein poly ADP-ribose polymerase (PARP)-a DNA repair enzyme in cell survival during I/..
  2. Role of troponin T isoforms in nemaline myopathy
    Jian Ping Jin; Fiscal Year: 2012
    ..This truncated slow TnT cannot incorporate into myofibrils and is degraded inside the myocyte...
  3. Cardiac dysfunction in StrepTSS: interplay of SLO and MMPs
    Dennis L Stevens; Fiscal Year: 2013
    ..Yet disassembled collagen scaffolding cannot function efficiently as structural support for myofibrils. Thus, MMP-13, induced by bacterial virulence factors such as SLO, may uniquely drive acute cardiac dysfunction ..
  4. Modulation of Ca Control of Cardiac Myofibrils
    R John Solaro; Fiscal Year: 2013
    ....
  5. Cullin mediated protein turnover in skeletal and cardiac muscles
    Stephan Lange; Fiscal Year: 2012
    ..Intriguingly, several components involved in the ubiquitylation of muscle proteins are localized along myofibrils, like the muscle specific E3-ligases of the MuRF protein family...
  6. Cullin mediated protein turnover in skeletal and cardiac muscles
    Stephan Lange; Fiscal Year: 2013
    ..Intriguingly, several components involved in the ubiquitylation of muscle proteins are localized along myofibrils, like the muscle specific E3-ligases of the MuRF protein family...
  7. Analysis of Z-band assembly and maintenance in living skeletal muscle cells
    JOSEPH WILLIAM SANGER; Fiscal Year: 2013
    Our long-term goals are to understand how sarcomeric proteins are assembled into myofibrils, and, how when mutated, truncated or deleted, these sarcomeric proteins result in myopathies...
  8. Role of Cardiac Troponin in Health and Disease
    JONATHAN PAUL DAVIS; Fiscal Year: 2013
    ..from TnC is not rapid in more physiologically relevant biochemical systems (reconstituted thin filaments and myofibrils) and is similar to the rate of cardiac muscle relaxation, 3) physiological and pathophysiological modifications ..
  9. MOLECULAR MECHANISMS OF OXIDATIVE STRESS IN AGING MUSCLE
    Christiaan Leeuwenburgh; Fiscal Year: 2012
    ..of mitochondria exist: interfibrillar mitochondria (IFM), which are found in parallel rows between the myofibrils, and subsarcolemmal mitochondria (SSM), which are located beneath the plasma membrane...
  10. INTEGRATED MECHANISMS OF CARDIAC MALADAPTATION
    R John Solaro; Fiscal Year: 2013
    ..of cardiac hemodynamics and metabolism with NMR, mechanical studies at the level of the heart, myocytes, and myofibrils, proteomics, and molecular studies at the protein level...
  11. Effect of Aging on Lateral Transmission of Force in Skeletal Muscle
    Yingxin Gao; Fiscal Year: 2012
    ..Transmitting forces from myofibrils to the tendon laterally involves several anatomical structures, including the myofibrils, the extracellular ..
  12. COOPERATIVE THIN FILAMENT ACTIVATION IN STRIATED MUSCLE
    Michael Regnier; Fiscal Year: 2012
    ..sophisticated solution biochemical techniques and functional analysis at the level of individual myofilaments, myofibrils cells and tissue...
  13. Kinetics of Cardiac Myofilament Activation
    Wen Ji Dong; Fiscal Year: 2013
    ..the structural changes and structural kinetics of the thin filament modified in the skinned muscle fibers and myofibrils? The underlying hypothesis of this proposal is that the Ca2+induced structural transitions involving the ..
  14. Thick and Thin Filament Mutations that cause Distal Arthrogryposis
    ALICE WARD RACCA; Fiscal Year: 2013
    ..I plan to do so with three aims, focusing on muscle contractility of muscle fibers and muscle myofibrils. I will use muscle fibers to determine maximal force production, crossbridge binding, and Ca2+ sensitivity of ..
  15. Roles of nebulin in structure and function of straited muscle
    Henk L Granzier; Fiscal Year: 2013
    ..This work includes study of force kinetics in single myofibrils and the effect of nebulin on crossbridge recruitment...
  16. MyBP-C slow variant 1: a slow isoform in a fast muscle
    MAEGEN ALLYSUM ACKERMANN; Fiscal Year: 2012
    DESCRIPTION (provided by applicant): Myofibrils, the workhorses of skeletal muscle, consist of interdigitating thick and thin filaments, their surrounding membrane systems and the proteins associated with each...
  17. Myofibril disassembly during neonatal heart muscle cell proliferation
    Bernhard Kuhn; Fiscal Year: 2013
    ..requires understanding of how cardiomyocytes are able to perform two completely different tasks: contraction of myofibrils and cell division...
  18. TROPOMYOSIN AND THE REGULATION OF MUSCLE CONTRACTION
    SHERWIN LEHRER; Fiscal Year: 2009
    ..energy transfer (FRET) in solution and in regions of the sarcomere of components exchanged into single myofibrils. These measurements between specific labeled amino acid residues will provide information about the dynamics of ..
  19. Studies on a Novel RNA that Promotes Heart Development
    LARRY LEMANSKI; Fiscal Year: 2006
    ..development, because homozygous embryos (c/c) form hearts that are deficient in tropomyosin, lack organized myofibrils, and fail to beat...
  20. Intermediate Filaments & Costamere Structure & Function
    Robert J Bloch; Fiscal Year: 2010
    Costameres are structures at the surface of striated muscle that align the sarcolemma regularly with nearby myofibrils and transmit contractile force laterally, through the membrane to the extracellular matrix...
  21. The Sarcolemma in FSHD and in the myd Mouse
    Robert Bloch; Fiscal Year: 2003
    ..the sarcolemma of FSHD muscle has frequent interruptions in its membrane skeleton, is separated from the nearest myofibrils by a considerable gap, and is organized irregularly, and most closely resembles the sarcolemma of slow twitch ..
  22. ALTERED MATRIX METALLOPROTEINASES IN HEART FAILURE
    JEANINE D ARMIENTO; Fiscal Year: 2002
    ..integrity and tensile strength to the myocardium critical to maintaining the alignment of myocytes and myofibrils, and the overall geometry of the left ventricle...
  23. FROM CARDIAC MUSCLE TO VENTRICLE
    William Hunter; Fiscal Year: 1999
    ..The project will test 3 hypotheses: (1) Mechanical coupling between active myofibrils and the cytoskeletal-extracellular matrix causes significant stiffening of systolic myocardium against shear and ..
  24. IMMUNOCYTOCHEMICAL STUDY OF CARDIAC MUSCLE ORGANIZATION
    KIYOTERU TOKUYASU; Fiscal Year: 1990
    ..The positions of both the myofibrils and the fasciae adherentes into which the myofibrils terminate must therefore be precisely fixed in space...
  25. GENE EXPRESSION DURING DROSOPHILA MYOGENESIS
    Allen Shearn; Fiscal Year: 1999
    ..We will use polarized light microscopy and anti-myosin antibody decoration to recognize mutants having defective myofibrils. Since the locations of all contractile protein genes of Drosophila are known, this project will provide an ..
  26. NOVEL PROTEIN ASSOCIATED WITH HEART DEVELOPMENT
    LARRY LEMANSKI; Fiscal Year: 2001
    ..Homozygosity for the lethal cardiac mutant allele in Mexican axolotls results in failure to develop organized myofibrils and contracting hearts...
  27. STRUCTURE, FUNCTION & MECHANISM OF CONTRACTILE PROTEINS
    YALE GOLDMAN; Fiscal Year: 2002
    ..activity, molecular motions and reaction kinetics in isolated contractile protein molecules fully constituted myofibrils. The objective is to understand how these proteins, individually and organized in highly specific, ..
  28. EARLY DETECTION OF DIABETIC CARDIOMYOPATHY WITH MRI
    Christine Lorenz; Fiscal Year: 1999
    ..motion of the ventricle referred to as torsion reflects the underlying shearing motion of individual planes of myofibrils which generates wall thickening and ventricular ejection...
  29. ANTHRACYCLINE CARDIOTOXICITY--OXIDATIVE STRESS
    Narine Sarvazyan; Fiscal Year: 2002
    ..administration is associated with redistribution of the protein kinase C epsilon isoform from cytosol to myofibrils, and 3) detected disruption of the periodicity of actin staining after repetitive exposure of myocytes to ..
  30. MECHANO-CHEMICAL PROPERTIES OF SKINNED CARDIAC MYOCYTES
    TATSUO IWAZUMI; Fiscal Year: 1992
    ..These were originally aimed at measuring the mechanics of single myofibrils, but the methodology was recently modified to suit for myocytes and ultra-thin trabeculae...
  31. STRUCTURAL ASSEMBLY IN MUSCLE AND NON-MUSCLE CELLS
    Yu li Wang; Fiscal Year: 1993
    ..The ability and pattern of incorporation into leading lamellipodia, contractile rings, and developing myofibrils will be analyzed...
  32. Cyclin Dependent Kinase 6 in Cardiac Development
    Hal Skopicki; Fiscal Year: 2006
    ..of cdk6 can be effected in vitro by TGFbeta; 4) In vivo co- localization of cdk6 with developing cytoplasmic myofibrils ; and 5) Specific binding of cdk6 to myosin heavy chain with the maturing sarcomere...
  33. MOLECULAR/CELLULAR BIOLOGY OF CARDIOMYOCTE DEVELOPMENT
    DONALD FISCHMAN; Fiscal Year: 2000
    ..program is to understand the morphogenesis of embryonic cardiac muscle in vivo, particularly the assembly of myofibrils in that tissue...
  34. Mechanisms of E-C Coupling in Atrial Cells
    C Balke; Fiscal Year: 2006
    ..the cell interior that triggers a near synchronous release of C2+ from the SR and subsequent activation of the myofibrils throughout the cell...
  35. TROPOMODULIN FUNCTION AND CARDIAC MYOFIBRIL ASSEMBLY
    Carol Gregorio; Fiscal Year: 2006
    DESCRIPTION (provided by applicant): The formation of perfectly aligned myofibrils in cardiac muscle is a dramatic example of supramolecular assembly in eukaryotic cells; the mechanisms by which this occur are still incompletely ..
  36. Obscurin Signaling Through its Rho-GEF and PH Domains
    AMBER BOWMAN; Fiscal Year: 2007
    ..During embryonic development, sarcomeres are assembled and organized to form myofibrils. Defects in the proteins that form the sarcomeres and the cytoskeleton that supports the contractile apparatus ..
  37. STRESS AND STRAIN AS STIMULI FOR MYOCARDIAL GROWTH
    Jeffrey Omens; Fiscal Year: 1999
    ..Furthermore, the applicant proposes that cytoskeletal components play a key role in the growth response of the myofibrils and will test this hypothesis by disrupting certain cytoskeletal structures and documenting the growth response...
  38. Physiological Role of the Myosin Regulatory Light Chain
    James Potter; Fiscal Year: 2005
    ..g., myofibrils and skinned muscle fibers and this has contributed to the controversy...
  39. TROPOMODULIN FUNCTION IN HEART DEVELOPMENT
    Carol Gregorio; Fiscal Year: 2003
    The formation of perfectly aligned myofibrils in cardiac muscle represents a dramatic examine of supramolecular assembly in eukaryotic cells; the mechanisms by which this occurs are still incompletely understood...
  40. Mechanical role of titin in limiting eccentric muscle damage
    Emanuel Azizi; Fiscal Year: 2010
    ..These eccentric contractions can damage myofibrils and often result in severe delayed-onset muscle soreness...
  41. Myosin-based kinetic differences between muscle types
    David Maughan; Fiscal Year: 2006
    ..isoforms? To answer these questions (framed as hypotheses), we will contrast kinetic schemes obtained from myofibrils of indirect flight muscle (IFM, an extremely fast muscle), jump muscle (a fast muscle), IFM myofibrils ..
  42. IN VITRO STUDIES OF THICK FILAMENT ASSEMBLY
    ANURADHA SAAD; Fiscal Year: 1990
    A preeminent feature of muscle differentiation is the morphogenesis of myofibrils and the onset of contractility...
  43. HIV-I PROTEINS AND RNA IN THE HEART OF AIDS PATIENTS
    Jiang Gu; Fiscal Year: 1991
    ..proteins pl7- and p24-like immunoreactivities and envelope protein gpl6O-like immunoreactivity were present in myofibrils of the myocardiocytes in the heart of HIV infected patients (n=5) but not of non AIDS patients (n=6)...
  44. Calcium Waves in Atrial Cells
    Leighton Izu; Fiscal Year: 2005
    ..triggering a near synchronous calcium (Ca) release from the sarcoplasmic reticulum (SR) and activation of myofibrils throughout the cell. Atrial cells lack an extensive t-tubular system...
  45. GLYCOPROTEINS MEDIATING MYOBLAST RECOGNITION/FUSION
    Karen Knudsen; Fiscal Year: 1991
    ..and fuse to form multinucleate cells that synthesize proteins necessary for the assembly of striated myofibrils. One event previously shown by the applicant to be critical to this multistep process is the specific, Ca++-..
  46. IMAGING MELANIN IN MELANOCYTES
    Julian Borejdo; Fiscal Year: 2003
    ..The modifications are relatively simple, inexpensive and not tied to a specific brand of instrument. It is expected that the method will find application in a small diagnostic laboratory. ..
  47. Rotation of Single Cross-Bridge in Skeletal Muscle Fiber
    Julian Borejdo; Fiscal Year: 2007
    ..This is expected to provide definitive answers about the mechanism of contraction of skeletal muscle. ..
  48. Improved Confocal Microscope: Detection & Functionality
    Julian Borejdo; Fiscal Year: 2005
    ..To facilitate a future commercialization, a microscope maintenance/modification company that is interested in distribution of the product version of the modification package has been identified. ..
  49. M-Line Proteins and A-Band Assembly in Skeletal Muscle
    Aikaterini Kontrogianni Konstantopoulos; Fiscal Year: 2010
    ..abstract_text> ..
  50. INSECT PROJECTIN: ROLES IN SARCOMERE ASSEMBLY & FLIGHT
    Agnes Ayme Southgate; Fiscal Year: 2005
    The complex differentiation program undergone by muscle cells includes the assembly of myofibrils that involves the timely interactions of many proteins...
  51. MYOFIBRILLOGENESIS IN LIVING SKELETAL MUSCLE CELLS
    Joseph Sanger; Fiscal Year: 2004
    DESCRIPTION (adapted from investigator's abstract): Central to the form and function of muscles are the myofibrils that provide the cytoskeletal structure and the contractile force that characterize skeletal muscles...
  52. Purchase of Deconvolution Microscope System
    Joseph Sanger; Fiscal Year: 2003
    ..A deconvolution microscope system would open up exciting new possibilities for all of these biologists. ..
  53. CHANGES IN GENE EXPRESSION IN SENESCENT MUSCLE
    Stephen Welle; Fiscal Year: 2003
    ..abstract_text> ..
  54. STRUCTURAL BASIS OF SMOOTH MUSCLE CONTRACTION
    Roger Craig; Fiscal Year: 2002
    ..Preliminary data are presented showing the feasibility of our goals. ..
  55. C PROTEIN AND CROSS BRIDGE FUNCTION IN HEART MUSCLE
    Saul Winegrad; Fiscal Year: 2004
    ..Abnormalities in its structure and function are responsible for one of the more common forms of Familial Hypertrophic Cardiomyopathy. ..
  56. CCD Digital Imaging System for Core EM Facility
    Roger Craig; Fiscal Year: 2004
    ..A third TEM will continue to operate with film for researchers who prefer this medium. ..
  57. Disease and Continuous Myofiber Remodeling in EOM
    Linda McLoon; Fiscal Year: 2004
    ..Ultimately, we hope to use this information to develop new therapeutic strategies.for the treatment of strabismus and other ocular and non-ocular muscle diseases. ..
  58. Myofibril damage during low-flow ischemia
    Robert Decker; Fiscal Year: 2005
    ..the chronically instrumented canine heart demonstrate that viable cardiac myocytes disclose areas of disrupted myofibrils when flow and function remain mismatched...
  59. MYOFIBRILLOGENESIS IN LIVING CARDIAC MUSCLE CELLS
    Joseph Sanger; Fiscal Year: 2006
    A key subunit of myofibrils, the Z-band, links the sarcomeric contractile units in series and also serves as attachment sites for the intermediate filaments, the costameres and membranes...
  60. Muscle protein synthesis and gene expression in myostatin-deficient mice
    Stephen L Welle; Fiscal Year: 2010
    ..Knowledge of the physiological and molecular effects of reducing myostatin activity in mature muscle will reveal potential benefits and limitations of anti-myostatin therapy. ..
  61. Scanning Electron Microscope for Core EM Facility
    Roger Craig; Fiscal Year: 2007
    ..Many have a direct bearing on the understanding of human disease. Results provided by this instrument will play a key role in advancing our knowledge in these areas. [unreadable] [unreadable] [unreadable]..
  62. Dynamics of Proteins in the A-bands of Cardiac Muscle
    Joseph Sanger; Fiscal Year: 2009
    ..We have proposed a three-step model for the formation of myofibrils in muscle cells: premyofibrils to nascent myofibrils to mature myofibrils...
  63. BIACORE 3000 Biosensor
    Robert Bloch; Fiscal Year: 2002
    ..We anticipate that this facility will provide its primary users, and ultimately others at the SoM and in the Baltimore area, with state-of-the-art biosensor technology. ..
  64. MECHANOENERGETICS OF HYPERTROPHY AND FAILURE
    Martin LeWinter; Fiscal Year: 2006
    ..This approach, combined with Aims I and II, should provide novel information about the details of thin filament phosphorylation in failing myocardium and may suggest new therapeutic targets. ..