Genomes and Genes
Summary: Highly organized bundles of actin (=ACTINS), MYOSINS, and other proteins in the cytoplasm of skeletal and cardiac muscle cells that contract by a sliding filament mechanism.
Publications191 found, 100 shown here
- Transient association of titin and myosin with microtubules in nascent myofibrils directed by the MURF2 RING-finger proteinVéronique Pizon
European Molecular Biology Laboratory, Cell Biology Division, Heidelberg, Germany
J Cell Sci 115:4469-82. 2002..microtubules associate transiently with sarcomeric myosin and later with A-band titin when non-striated myofibrils differentiate into mature sarcomeres...
- Modulation of energy transfer pathways between mitochondria and myofibrils by changes in performance of perfused heartMarko Vendelin
Laboratory of Systems Biology, Institute of Cybernetics, Tallinn University of Technology, Akadeemia 21, 12618 Tallinn, Estonia
J Biol Chem 285:37240-50. 2010In the heart, the energy supplied by mitochondria to myofibrils is continuously and finely tuned to the contraction requirement over a wide range of cardiac loads...
- Muscle-specific RING finger-1 interacts with titin to regulate sarcomeric M-line and thick filament structure and may have nuclear functions via its interaction with glucocorticoid modulatory element binding protein-1Abigail S McElhinny
Department of Cell Biology and Anatomy, University of Arizona, Tucson, AZ 85724, USA
J Cell Biol 157:125-36. 2002..The dual interactions of MURF-1 with titin and GMEB-1 may link myofibril signaling pathways (perhaps including titin's kinase domain) with muscle gene expression...
- SmyD1, a histone methyltransferase, is required for myofibril organization and muscle contraction in zebrafish embryosXungang Tan
Center of Marine Biotechnology, University of Maryland Biotechnology Institute, Baltimore, MD 21202, USA
Proc Natl Acad Sci U S A 103:2713-8. 2006..Together, these data indicate that SmyD1a and SmyD1b are histone methyltransferases and play a critical role in myofibril organization during myofiber maturation...
- Myofibrillar myopathies: a clinical and myopathological guideRolf Schroder
Institute of Neuropathology and Department of Neurology, University Hospital Erlangen, Erlangen, Germany
Brain Pathol 19:483-92. 2009..This review focuses on the clinical and myopathological aspects of genetically defined MFMs, and shall provide a diagnostic guide for this numerically significant group of protein aggregate myopathies...
- MURF-1 and MURF-2 target a specific subset of myofibrillar proteins redundantly: towards understanding MURF-dependent muscle ubiquitinationStephanie H Witt
Institut fur Anasthesiologie und Operative Intensivmedizin, Universitatsklinikum Mannheim, Mannheim 68167, Germany
J Mol Biol 350:713-22. 2005..These data raise the possibility that MURF-1 may coordinately regulate the energy metabolism of mitochondrial and cytoplasmic compartments...
- Transverse stiffness of myofibrils of skeletal and cardiac muscles studied by atomic force microscopyNao Akiyama
Department of Physics (Biophysics Section, Faculty of Science, Tokyo University of Science, Tokyo, Japan
J Physiol Sci 56:145-51. 2006The transverse stiffness of single myofibrils of skeletal and cardiac muscles was examined by atomic force microscopy...
- Knockdown and overexpression of Unc-45b result in defective myofibril organization in skeletal muscles of zebrafish embryosElena P Bernick
University of Maryland School of Medicine Interdisciplinary Training Program in Muscle Biology, Baltimore, MD 21201, USA
BMC Cell Biol 11:70. 2010..The effects of unc-45b knockdown on other sarcomeric structures and the phenotype of Unc-45b overexpression, however, are poorly understood in vertebrates...
- Specific interaction of the potassium channel beta-subunit minK with the sarcomeric protein T-cap suggests a T-tubule-myofibril linking systemT Furukawa
Department of Physiology, Akita University School of Medicine, Japan
J Mol Biol 313:775-84. 2001..Striated staining of minK was observed in non-washed, membrane-intact cardiac myofibrils, but not in well-washed, membrane-removed cardiac myofibrils, suggesting that minK localizes on T-tubular ..
- Targeted homozygous deletion of M-band titin in cardiomyocytes prevents sarcomere formationHanny Musa
Institute for Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds, Leeds, LS2 9JT, UK
J Cell Sci 119:4322-31. 2006..These results show that integration of the M-band region of titin is required for myosin filament assembly, M-band formation and maturation of the Z-disk...
- Protein kinase G modulates human myocardial passive stiffness by phosphorylation of the titin springsMartina Kruger
Physiology and Biophysics Unit, University of Muenster, Germany
Circ Res 104:87-94. 2009..determine whether PKG can phosphorylate titin and affect titin-based stiffness in skinned myofibers and isolated myofibrils. PKG in the presence of 8-pCPT-cGMP (cGMP) phosphorylated the 2 main cardiac titin isoforms, N2BA and N2B, in ..
- A new 185,000-dalton skeletal muscle protein detected by monoclonal antibodiesB K Grove
J Cell Biol 98:518-24. 1984..antibodies (mAbs) against a 165-kd fraction, we also obtained mAbs that bound to the M line of isolated myofibrils as detected by indirect immunofluorescence, but recognized a protein band of 185 kd in immunoblotting ..
- Interaction between PEVK-titin and actin filaments: origin of a viscous force component in cardiac myofibrilsM Kulke
Institute of Physiology, University of Heidelberg, Heidelberg, Germany
Circ Res 89:874-81. 2001..To probe the interaction in a more physiological environment, nonactivated cardiac myofibrils were stretched quickly, and force was measured during the subsequent hold period...
- Binding of an ankyrin-1 isoform to obscurin suggests a molecular link between the sarcoplasmic reticulum and myofibrils in striated musclesPaola Bagnato
Molecular Medicine Section, Department of Neuroscience, University of Siena, 53100 Siena, Italy
J Cell Biol 160:245-53. 2003..The mechanisms that mediate the structural organization of the sarcoplasmic reticulum with respect to the myofibrils are, however, not known. We report here that ank1...
- Structural basis for activation of the titin kinase domain during myofibrillogenesisO Mayans
European Molecular Biology Laboratory, Hamburg Outstation, Germany
Nature 395:863-9. 1998..Titin kinase phosphorylates the muscle protein telethonin in early differentiating myocytes, indicating that this kinase may act in myofibrillogenesis...
- Lys184 deletion in troponin I impairs relaxation kinetics and induces hypercontractility in murine cardiac myofibrilsBogdan Iorga
Institute of Vegetative Physiology, University of Cologne, Robert Koch Strasse 39, Cologne, Germany
Cardiovasc Res 77:676-86. 2008....
- Myofibrillar architecture in engineered cardiac myocytesKevin Kit Parker
Disease Biophysics Group, School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA kkparker seas harvard edu
Circ Res 103:340-2. 2008..These data suggest that physical constrainment of muscle cells by extracellular matrix may be an important regulator of myofibrillar organization...
- Differential involvement of sarcomeric proteins in myofibrillar myopathies: a morphological and immunohistochemical studyKristl G Claeys
Institut de Myologie, Groupe Hospitalier Pitie Salpetriere, Paris, France
Acta Neuropathol 117:293-307. 2009....
- Functional properties of the titin/connectin-associated proteins, the muscle-specific RING finger proteins (MURFs), in striated muscleCarol C Gregorio
Department of Cell Biology and Anatomy, University of Arizona, Tucson, AZ 85724, USA
J Muscle Res Cell Motil 26:389-400. 2005..is dependent upon the proper alignment and coordinated activities of several cytoskeletal networks including myofibrils, microtubules, and intermediate filaments...
- Distinct mechanisms for diastolic dysfunction in diabetes mellitus and chronic pressure-overloadInes Falcao-Pires
Department of Physiology and Cardiothoracic Surgery, Cardiovascular R and D Unit, Faculty of Medicine, University of Porto, Alameda Professor Hernani Monteiro, 4200 319 Porto, Portugal
Basic Res Cardiol 106:801-14. 2011..The association of these damages accelerates the progression of diastolic heart failure progression in diabetic-banded animals...
- Insights into the kinetics of Ca2+-regulated contraction and relaxation from myofibril studiesRobert Stehle
Institute of Physiology, University of Cologne, Robert Koch Str 39, 50931 Cologne, Germany
Pflugers Arch 458:337-57. 2009..techniques that analyse the chemical and mechanical kinetics of small components of a muscle fibre, subcellular myofibrils isolated from skeletal and cardiac muscle...
- Myofibrillar calcium sensitivity of isometric tension is increased in human dilated cardiomyopathies: role of altered beta-adrenergically mediated protein phosphorylationM R Wolff
Department of Medicine, University of Wisconsin, Madison 53792, USA
J Clin Invest 98:167-76. 1996....
- Myofibrillogenesis in the first cardiomyocytes formed from isolated quail precardiac mesodermAiping Du
Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104-6058, USA
Dev Biol 257:382-94. 2003De novo assembly of myofibrils was investigated in explants of precardiac mesoderm from quail embryos to address a controversy about different models of myofibrillogenesis...
- Myofibrillar myopathiesDuygu Selcen
Department of Neurology, Division of Child Neurology and Neuromuscular Disease Research Laboratory, Mayo Clinic College of Medicine, Mayo Clinic, Rochester, MN, USA
Neuromuscul Disord 21:161-71. 2011..However, in the majority of the myofibrillar myopathy patients the disease gene awaits discovery...
- Assembly and dynamics of myofibrilsJoseph W Sanger
Department of Cell and Developmental Biology, SUNY Upstate Medical University, Syracuse, NY 13210, USA
J Biomed Biotechnol 2010:858606. 2010We review some of the problems in determining how myofibrils may be assembled and just as importantly how this contractile structure may be renewed by sarcomeric proteins moving between the sarcomere and the cytoplasm...
- Why choose myofibrils to study muscle myosin ATPase?Corinne Lionne
UMR 5121, CNRS Université Montpellier I, Institut de Biologie, 4 boulevard Henri IV CS89508, 34960 Montpellier 2, France
J Muscle Res Cell Motil 24:139-48. 2003..In the presence of calcium, myofibrillar ATPase is activated and myofibrils either shorten with no external load (native myofibrils) or contract isometrically (cross-linked myofibrils)...
- Actin-titin interaction in cardiac myofibrils: probing a physiological roleW A Linke
Institute of Physiology II, University of Heidelberg, Germany
Biophys J 73:905-19. 1997The high stiffness of relaxed cardiac myofibrils is explainable mainly by the expression of a short-length titin (connectin), the giant elastic protein of the vertebrate myofibrillar cytoskeleton...
- Myofibrillogenesis in the developing zebrafish heart: A functional study of tnnt2Wei Huang
Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine, Rochester, MN 55905, USA
Dev Biol 331:237-49. 2009..In summary, our data indicates that zebrafish are a valuable in vivo model for studying both myofibrillogenesis and sarcomere-based cardiac diseases...
- Form follows function: how muscle shape is regulated by workB Russell
Department of Physiology and Biophysics, College of Medicine, University of Illinois at Chicago, Chicago, Illinois 60612, USA
J Appl Physiol 88:1127-32. 2000..of cell shape and the composition and aggregation of contractile proteins into filaments, the sarcomere, and myofibrils. We examine how mechanical activity regulates the turnover and exchange of contraction proteins...
- The M-band: an elastic web that crosslinks thick filaments in the center of the sarcomereIrina Agarkova
Institute of Cell Biology, Swiss Federal Institute of Technology, ,
Trends Cell Biol 15:477-85. 2005..We propose that the M-band is in fact a dynamic structure that monitors the stress appearing in the thick filament lattice during contraction and quickly reorganizes to meet new physiological requirements...
- Glucose and palmitic acid induce degeneration of myofibrils and modulate apoptosis in rat adult cardiomyocytesD Dyntar
Division of Endocrinology and Diabetes, University Hospital, Zurich, Switzerland
Diabetes 50:2105-13. 2001..C2-dihydroceramide enhanced apoptosis of cardiomyocytes by 50%, accompanied by detrimental effects on the myofibrils. The palmitic acid-induced effects were impaired by fumonisin B1, an inhibitor of ceramide synthase...
- Mechanical stress-strain sensors embedded in cardiac cytoskeleton: Z disk, titin, and associated structuresMasahiko Hoshijima
Department of Medicine, University of California, San Diego, La Jolla, CA 92093 0734, USA
Am J Physiol Heart Circ Physiol 290:H1313-25. 2006..This review summarizes current knowledge of this rapidly developing area with focus on how the heart adjusts physiological remodeling process to meet with mechanical demands and how this process fails in cardiac pathologies...
- Aberrant myofibril assembly in tropomodulin1 null mice leads to aborted heart development and embryonic lethalityKimberly L Fritz-Six
Department of Cell Biology, The Scripps Research Institute, 10550 N Torrey Pines Road, La Jolla, CA 92037, USA
J Cell Biol 163:1033-44. 2003..5. Confocal microscopy of hearts of E8-8.5 Tmod1 null embryos revealed structures resembling nascent myofibrils with continuous F-actin staining and periodic dots of alpha-actinin, indicating that I-Z-I complexes assembled ..
- Nebulin-deficient mice exhibit shorter thin filament lengths and reduced contractile function in skeletal muscleMarie Louise Bang
Department of Medicine, Veterans Affairs Medical Center, University of California, San Diego, La Jolla, CA 92093, USA
J Cell Biol 173:905-16. 2006..The functional importance of nebulin in skeletal muscle function was revealed by isometric contractility assays, which demonstrated a dramatic reduction in force production in nebulin-deficient skeletal muscle...
- Tropomodulin1 is required in the heart but not the yolk sac for mouse embryonic developmentCaroline R McKeown
The Scripps Research Institute, Department of Cell Biology, La Jolla, CA 92037, USA
Circ Res 103:1241-8. 2008Tropomodulin (Tmod)1 caps the pointed ends of actin filaments in sarcomeres of striated muscle myofibrils and in the erythrocyte membrane skeleton...
- Molecular diversity of myofibrillar proteins: gene regulation and functional significanceS Schiaffino
Department of Biomedical Sciences, University of Padova, Padua, Italy
Physiol Rev 76:371-423. 1996..Both myosin and troponin isoforms contribute to the differences in the resistance to fatigue of muscle fibers...
- Obscurin is required for the lateral alignment of striated myofibrils in zebrafishMaide O Raeker
Department of Pediatrics and Communicable Diseases, University of Michigan, Ann Arbor, Michigan 48109, USA
Dev Dyn 235:2018-29. 2006..independent morpholino antisense oligonucleotides resulted in diminished numbers and marked disarray of skeletal myofibrils, impaired lateral alignment of adjacent myofibrils, disorganization of the sarcoplasmic reticulum, somite ..
- Myofibrillar myopathy: clinical, morphological and genetic studies in 63 patientsDuygu Selcen
Department of Neurology, Neuromuscular Research Laboratory, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA
Brain 127:439-51. 2004..Further advances in defining the molecular causes of MFM will probably come from linkage studies of informative kinships or from systematic search for mutations in proteins participating in the intricate network supporting the Z-disk...
- Myofibrillogenesis in skeletal muscle cells in zebrafishJoseph W Sanger
Department of Cell and Developmental Biology, SUNY Upstate Medical University, Syracuse, New York, USA
Cell Motil Cytoskeleton 66:556-66. 2009..model of myofibrillogenesis, based on observations in cultured avian muscle cells, proposes that mature myofibrils are preceded by two intermediary structures: premyofibrils and nascent myofibrils...
- Myosin transducer mutations differentially affect motor function, myofibril structure, and the performance of skeletal and cardiac musclesAnthony Cammarato
Department of Biology and Heart Institute, San Diego State University, San Diego, CA 92182 4614, USA
Mol Biol Cell 19:553-62. 2008..Our data suggest Drosophila is a valuable system for identifying and modeling mutations analogous to those associated with specific human muscle disorders...
- Cardiac myofibrillogenesis inside intact embryonic heartsAiping Du
Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 6058, USA
Dev Biol 318:236-46. 2008How proteins assemble into sarcomeric arrays to form myofibrils is controversial...
- How to build a myofibrilJoseph W Sanger
Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 6058, USA
J Muscle Res Cell Motil 26:343-54. 2005..The models hypothesize: (a) stress fiber-like structures as templates for the assembly of myofibrils, (b) assembly in which the actin filaments and Z-bands form subunits independently from A-band subunits, with ..
- Titin: major myofibrillar components of striated muscleK Wang
Proc Natl Acad Sci U S A 76:3698-702. 1979..proteins, we purified a combination of two uncommonly large proteins, designated as titin, from chicken breast myofibrils. Chemical and immunological studies indicated that titin is distinct from myosin, actin, and filamin...
- Selective deletion of the NH2-terminal variable region of cardiac troponin T in ischemia reperfusion by myofibril-associated mu-calpain cleavageZhiling Zhang
Section of Molecular Cardiology, Evanston Northwestern Healthcare and Northwestern University Feinberg School of Medicine, Evanston, Illinois 60201, USA
Biochemistry 45:11681-94. 2006..in myocardial ischemia reperfusion, calpain inhibitors decreased the level of cTnT-ND in Triton-extracted myofibrils. Mu-calpain treatment of the cardiac myofibril and troponin complex specifically reproduced cTnT-ND...
- Isolation and characterization of flightless mutants in Drosophila melanogasterT Koana
J Embryol Exp Morphol 45:123-43. 1978..The indirect flight muscle of flltH was also examined, and it was found that sarcomere length and diameter of myofibrils were abnormal...
- Intercellular and extracellular mechanotransduction in cardiac myocytesJ Yasha Kresh
Department of Cardiothoracic Surgery, Drexel University College of Medicine, 245 North 15th Street, MS 111, Philadelphia, PA 19102, USA
Pflugers Arch 462:75-87. 2011....
- Pathogenity of some limb girdle muscular dystrophy mutations can result from reduced anchorage to myofibrils and altered stability of calpain 3Natalia Ermolova
Department of Neurology, David Geffen School of Medicine at University of California, Los Angeles, CA 90095, USA
Hum Mol Genet 20:3331-45. 2011..Our data suggest that R448H and D705G mutations affect both CAPN3s anchorage to titin and its stability. These studies reveal a novel mechanism by which mutations that spare enzymatic activity can still lead to calpainopathy...
- Late-stage alterations in myofibrillar contractile function in a transgenic mouse model of dilated cardiomyopathy (Tgalphaq*44)István Ferenc Edes
Division of Clinical Physiology, Institute of Cardiology University of Debrecen, Medical and Health Science Center, Faculty of Medicine Móricz Zs krt 22 H 4032 Debrecen, Hungary
J Mol Cell Cardiol 45:363-72. 2008..In conclusion, our data suggested that an initial decrease of PKA signaling and subsequent changes in myofilament protein expression may contribute to the development of dilated cardiomyopathy in Tgalphaq*44 hearts...
- Susceptibility of isolated myofibrils to in vitro glutathionylation: Potential relevance to muscle functionsChiara Passarelli
Children s Hospital and Research Institute Bambino Gesu, Rome, Italy
Cytoskeleton (Hoboken) 67:81-9. 2010In this study we investigated the molecular mechanism of glutathionylation on isolated human cardiac myofibrils using several pro-glutathionylating agents...
- Extralysosomal protein degradation in myofibrillar myopathiesMontse Olive
Institut de Neuropatologia, IDIBELL Hospital Universitari de Bellvitge, Hospitalet de Llobregat, Barcelona, Spain
Brain Pathol 19:507-15. 2009..a group of heterogeneous muscle disorders morphologically defined by the presence of foci of dissolution of the myofibrils, accumulation of the products of myofibrillar degradation and ectopic expression of multiple proteins...
- Myofibrillar myopathiesDuygu Selcen
Department of Neurology, Division of Child Neurology and Neuromuscular Research Laboratory, Mayo Clinic, College of Medicine Rochester, Minnesota, USA
Curr Opin Neurol 21:585-9. 2008..The aim of this communication is to provide an up-to-date overview of myofibrillar myopathies...
- Ranolazine improves cardiac diastolic dysfunction through modulation of myofilament calcium sensitivityJoshua D Lovelock
Section of Cardiology, University of Illinois at Chicago, 60612, USA
Circ Res 110:841-50. 2012..Oxidative stress has been shown to increase late inward sodium current (I(Na)), reducing the net cytosolic Ca(2+) efflux...
- Tension generation and relaxation in single myofibrils from human atrial and ventricular myocardiumNicoletta Piroddi
Centro Interuniversitario di Medicina Molecolare e Biofisica Applicata, Florence, Italy
Pflugers Arch 454:63-73. 2007Fast solution switching techniques in single myofibrils offer the opportunity to dissect and directly examine the sarcomeric mechanisms responsible for force generation and relaxation...
- Effects of the mutation R145G in human cardiac troponin I on the kinetics of the contraction-relaxation cycle in isolated cardiac myofibrilsM Kruger
Department of Vegetative Physiology, University of Cologne, Koeln, Germany
J Physiol 564:347-57. 2005..of the mutation hcTnI(R145G) on crossbridge kinetics, force kinetics were analysed in murine cardiac myofibrils carrying either the mutant or the wild-type protein...
- Evidence that phosphate release is the rate-limiting step on the overall ATPase of psoas myofibrils prevented from shortening by chemical cross-linkingCorinne Lionne
INSERM U128, 1919 Route de Mende, 34293 Montpellier Cedex 5, France
Biochemistry 41:13297-308. 2002..We have put this idea to the test with myofibrils as a model because with these both mechanical and chemical kinetic measurements are possible...
- Monolayers of silver nanoparticles decrease photobleaching: application to muscle myofibrilsP Muthu
Department of Molecular Biology and Immunology, University of North Texas Health Science Center, Fort Worth, Texas 76107 2699, USA
Biophys J 95:3429-38. 2008..Skeletal muscle myofibrils were used as an example, because they contain submicron structures conveniently used to define optical ..
- Ultrastructural organization of the transverse tubules and the sarcoplasmic reticulum in a fish sound-producing muscleSuechika Suzuki
Department of Physiology, School of Medicine, Teikyo University, Itabashi ku, Tokyo 173 8605, Japan
J Electron Microsc (Tokyo) 52:337-47. 2003..These results are discussed in connection with the mechanism, by which the mechanical activity of the SBM muscle is neurally controlled...
- Effects of chronic atrial fibrillation on active and passive force generation in human atrial myofibrilsAlexandra Belus
Center of Molecular Medicine, Department of Physiology, University of Florence, Italy
Circ Res 107:144-52. 2010..Chronic atrial fibrillation (cAF) is associated with atrial contractile dysfunction. Sarcomere remodeling may contribute to this contractile disorder...
- Localization of the human 64kD autoantigen D1 to myofibrils in a subset of extraocular muscle fibersC A Conley
Department of Cell Biology, MB24, The Scripps Research Institute 10550 N Torrey Pines Rd, CA 92037, La Jolla, USA
Curr Eye Res 19:313-22. 1999..To evaluate the tissue-specific expression pattern of the 64kD human autoantigen D1, a tropomodulin-related protein that may be involved in thyroid-associated ophthalmopathy...
- Protein degradation by the ubiquitin-proteasome pathway in normal and disease statesStewart H Lecker
Nephrology Division, Beth Isreal Deaconess, Harvard Medical School, Boston, Massachusetts, USA
J Am Soc Nephrol 17:1807-19. 2006
- Paralysis and delayed Z-disc formation in the Xenopus tropicalis unc45b mutant dicky tickerTimothy J Geach
Division of Developmental Biology, MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK
BMC Dev Biol 10:75. 2010..Chaperone proteins specific to sarcomeric myosins have been shown to be necessary in zebrafish and invertebrates for proper muscle assembly and function...
- Assembly and remodelling of myofibrils and intercalated discs in cultured neonatal rat heart cellsB T Atherton
Department of Cell Biology and Anatomy, Northwestern University Medical School, Chicago, Illinois 60611
J Cell Sci 86:233-48. 1986The reorganization of myofibrils and the re-formation of intercalated discs was examined in neonatal rat cardiac muscle cells during the first 72 h of culture...
- Autophagy is required to maintain muscle massEva Masiero
Dulbecco Telethon Institute, 35129 Padova, Italy
Cell Metab 10:507-15. 2009..Our results suggest that inhibition/alteration of autophagy can contribute to myofiber degeneration and weakness in muscle disorders characterized by accumulation of abnormal mitochondria and inclusions...
- Expression of protein kinase C isoforms and interleukin-1beta in myofibrillar myopathyG Vattemi
Department of Neurological Sciences and Vision, Section of Clinical Neurology, Clinica Neurologica, Policlinico G B Rossi, Verona, Italy
Neurology 62:1778-82. 2004..The pathogenesis of this myopathy is not well understood. The morphologic hallmark is myofibrillar destruction with abnormal expression of numerous proteins, most consistently of desmin...
- Isometric force kinetics upon rapid activation and relaxation of mouse, guinea pig and human heart muscle studied on the subcellular myofibrillar levelRobert Stehle
Institute of Physiology, Universitat zu Koln, Robert Koch Str 39, 50931 Koln, Germany
Basic Res Cardiol 97:I127-35. 2002..of force development and relaxation upon rapid application and removal of Ca2+ was measured in bundles of few myofibrils isolated from triton X-100 skinned left ventricular trabeculae of mice (M), guinea pigs (G) and humans (H)...
- Ultrastructural and biochemical localization of N-RAP at the interface between myofibrils and intercalated disks in the mouse heartJ Q Zhang
Laboratory of Muscle Biology, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
Biochemistry 40:14898-906. 2001..addition, immunofluorescence shows that N-RAP remains bound at the ends of isolated, detergent-treated cardiac myofibrils. These results demonstrate that N-RAP remains tightly bound to myofibrils and fasciae adherentes during ..
- Dynamic behaviour of half-sarcomeres during and after stretch in activated rabbit psoas myofibrils: sarcomere asymmetry but no 'sarcomere popping'I A Telley
Laboratory for Biomechanics, , , HCI E 357.1, , Switzerland
J Physiol 573:173-85. 2006..changes of individual half-sarcomeres during and after stretch in actively contracting, single rabbit psoas myofibrils containing 10-30 sarcomeres...
- The NH2 terminus of titin spans the Z-disc: its interaction with a novel 19-kD ligand (T-cap) is required for sarcomeric integrityC C Gregorio
Departments of Cell Biology and Anatomy, and Molecular and Cellular Biology, University of Arizona, Tucson, Arizona 85724, USA
J Cell Biol 143:1013-27. 1998....
- Contractile effects of the exchange of cardiac troponin for fast skeletal troponin in rabbit psoas single myofibrilsN Piroddi
Dipartimento di Scienze Fisiologiche, , I-50134 Firenze, Italy
J Physiol 552:917-31. 2003..the exchange of fast skeletal troponin (fsTn) for cardiac troponin (cTn) were measured in rabbit fast skeletal myofibrils. Electrophoretic analysis of myofibril suspensions indicated that replacement of fsTnC or exchange of fsTn with ..
- A functional knock-out of titin results in defective myofibril assemblyP F van der Ven
Department of Cell Biology, University of Potsdam, Lennéstr 7a, D 14471 Potsdam, Germany
J Cell Sci 113:1405-14. 2000..The myofibroblast cell line BHK-21/C13 was described to assemble myofibrils in culture. We have now characterized the sub-line BHK-21-Bi, which bears a small deletion within the titin gene...
- Sequential myofibrillar breakdown accompanies mitotic division of mammalian cardiomyocytesPreeti Ahuja
Institute of Cell Biology, Swiss Federal Institute of Technology, , 8093 Zurich
J Cell Sci 117:3295-306. 2004..However, to go through cell division, a disassembly of the contractile elements, the myofibrils, has to take place...
- Myofibril degeneration caused by tropomodulin overexpression leads to dilated cardiomyopathy in juvenile miceM A Sussman
The Children s Hospital and Research Foundation, Cincinnati, Ohio 45229, USA
J Clin Invest 101:51-61. 1998..the heart compensates for myofibril degeneration, transgenic mice were created that undergo progressive loss of myofibrils after birth...
- SALS, a WH2-domain-containing protein, promotes sarcomeric actin filament elongation from pointed ends during Drosophila muscle growthJianwu Bai
Department of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA
Dev Cell 13:828-42. 2007..When sals is knocked down by RNAi, primary muscles display thin myofibrils with shortened sarcomeres and increased sarcomere number...
- Desmin is essential for the tensile strength and integrity of myofibrils but not for myogenic commitment, differentiation, and fusion of skeletal muscleZ Li
Station Centrale de Microscopie Electronique, Institut Pasteur, Paris, France
J Cell Biol 139:129-44. 1997..The results presented here show that desmin is essential to maintain the structural integrity of highly solicited skeletal muscle...
- Assembly of the giant protein projectin during myofibrillogenesis in Drosophila indirect flight musclesAgnes Ayme-Southgate
Department of Biology, College of Charleston, Charleston, South Carolina 29404, USA
BMC Cell Biol 5:17. 2004..It is comparable in its structure to titin, which has been implicated as a scaffold during vertebrate myofibrillogenesis...
- Protein import into subsarcolemmal and intermyofibrillar skeletal muscle mitochondria. Differential import regulation in distinct subcellular regionsM Takahashi
Departments of Biology and Kinesiology, York University, North York, Ontario M3J 1P3, Canada
J Biol Chem 271:27285-91. 1996....
- Genetics of the Drosophila flight muscle myofibril: a window into the biology of complex systemsJ O Vigoreaux
Department of Biology, University of Vermont, Burlington, VT 05405 USA
Bioessays 23:1047-63. 2001....
- Thin filaments elongate from their pointed ends during myofibril assembly in Drosophila indirect flight muscleM Mardahl-Dumesnil
Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
J Cell Biol 155:1043-53. 2001..Although pointed-end capping by Tmod controls thin filament lengths in assembled myofibrils, its role in length specification during de novo myofibril assembly is not established...
- Unfolding of titin domains explains the viscoelastic behavior of skeletal myofibrilsA Minajeva
Institute of Physiology and Pathophysiology, University of Heidelberg, D-69120 Heidelberg, Germany
Biophys J 80:1442-51. 2001..We show that isolated psoas myofibrils respond to a stretch-hold protocol with a characteristic force decay that becomes more pronounced following ..
- Genetic dissection of Drosophila myofibril formation: effects of actin and myosin heavy chain null allelesC J Beall
Department of Biology, Johns Hopkins University, Baltimore, Maryland 21218
Genes Dev 3:131-40. 1989..alleles have complex myofibrillar defects, whereas Mhc36B-/+; Act88F-/+ double heterozygotes have nearly normal myofibrils. These results imply that most defects observed in single heterozygotes are due to filament imbalances, not ..
- The Caenorhabditis elegans unc-78 gene encodes a homologue of actin-interacting protein 1 required for organized assembly of muscle actin filamentsS Ono
Department of Pathology, Emory University, Atlanta, Georgia 30322, USA
J Cell Biol 152:1313-9. 2001Assembly and maintenance of myofibrils require dynamic regulation of the actin cytoskeleton...
- Dynamics of actin and alpha-actinin in nascent myofibrils and stress fibersF Hasebe-Kishi
Department of Anatomy and Cell Biology, Chiba University School of Medicine, Japan
J Muscle Res Cell Motil 21:717-24. 2000..of these proteins, short spaced alpha-actinin dots adjoined each other and aligned with Z bands of neighboring myofibrils. It appears that both the difference in exchangeability between actin and alpha-actinin molecules and the ..
- Alterations in flight muscle ultrastructure and function in Drosophila tropomyosin mutantsA J Kreuz
Department of Molecular Genetics, Ohio State University, Columbus 43210, USA
J Cell Biol 135:673-87. 1996..In contrast, reduced expression of the TnH isoforms has an unexpectedly mild effect on IFM structure and function...
- A nebulin ruler does not dictate thin filament lengthsAngelica Castillo
Department of Forensic Science, Chaminade University, Honolulu, Hawaii, USA
Biophys J 96:1856-65. 2009....
- Drosophila D-titin is required for myoblast fusion and skeletal muscle striationY Zhang
Department of Biology, University of Utah, Salt Lake City, Utah 84112 0840, USA
J Cell Sci 113:3103-15. 2000..We propose that D-Titin is instrumental in the development of the two defining features of striated muscle: the formation of multi-nucleate syncitia and the organization of actin-myosin filaments into striated arrays...
- Decline in titin content in rat skeletal muscle after denervationSy-Ping Chen
Graduate Institute of Medical Sciences, Taipei Medical University, Taipei, Taiwan
Muscle Nerve 32:798-807. 2005..The ultrastructure of myofibrils also showed disturbed arrangements of myofilaments and a disorganized contractile apparatus in denervated muscle...
- Diaphragm dysfunction in chronic obstructive pulmonary diseaseCoen A C Ottenheijm
Department of Pulmonary Diseases, Radboud University Nijmegen Medical Centre, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands
Am J Respir Crit Care Med 172:200-5. 2005..Our data suggest that enhanced diaphragm protein degradation through the ubiquitin-proteasome pathway plays a role in loss of contractile protein and, consequently, failure of the diaphragm to generate force...
- No effect of menstrual cycle on myofibrillar and connective tissue protein synthesis in contracting skeletal muscleBenjamin F Miller
Institute of Sports Medicine, Copenhagen
Am J Physiol Endocrinol Metab 290:E163-E168. 2006..Therefore, there is no effect of menstrual cycle phase, at rest or in response to an acute bout of exercise, on myofibrillar protein synthesis and muscle collagen synthesis in women...
- Effect of heart failure on skeletal muscle myofibrillar protein content, isoform expression and calcium sensitivityMichael J Toth
Department of Medicine, University of Vermont, Burlington, VT 05405, United States
Int J Cardiol 107:211-9. 2006..To address this issue, we examined the effect of heart failure on skeletal muscle myofibrillar protein content, isoform distribution and Ca2+ sensitivity...
- Characterization of the myosin-based source for second-harmonic generation from muscle sarcomeresSergey V Plotnikov
Department of Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, Connecticut 06030-3301, USA
Biophys J 90:693-703. 2006..SHG from isolated myofibrils is abolished by extraction of myosin, but is unaffected by removal or addition of actin filaments...
- Skeletal muscle myofibrillar mRNA expression in heart failure: relationship to local and circulating hormonesMichael J Toth
Department of Medicine, Health Science Research Facility 126 B, 149 Beaumont Ave, University of Vermont, Burlington, VT 05405, USA
J Appl Physiol 100:35-41. 2006....
- Half-sarcomere dynamics in myofibrils during activation and relaxation studied by tracking fluorescent markersIvo A Telley
Laboratory for Biomechanics, , , Switzerland
Biophys J 90:514-30. 2006To study the dynamics of individual half-sarcomeres in striated muscle contraction, myofibrils prepared from rabbit psoas muscle and left ventricles of guinea pig were immunostained with two conjugated antibody complexes consisting of a ..
- The origin of passive force enhancement in skeletal muscleV Joumaa
Faculty of Kinesiology, University of Calgary, Calgary, Alberta, Canada
Am J Physiol Cell Physiol 294:C74-8. 2008..We measured passive force enhancement in troponin C (TnC)-depleted myofibrils in which active force production was completely eliminated...
- De novo myofibrillogenesis in C2C12 cells: evidence for the independent assembly of M bands and Z disksAikaterini Kontrogianni-Konstantopoulos
Dept of Physiology, School of Medicine, Univ of Maryland at Baltimore, 685 W Baltimore St, Baltimore, MD 21201, USA
Am J Physiol Cell Physiol 290:C626-37. 2006....
- In indirect flight muscles Drosophila projectin has a short PEVK domain, and its NH2-terminus is embedded at the Z-bandAgnes Ayme-Southgate
Department of Biology, College of Charleston, Charleston, SC 29424, USA
J Muscle Res Cell Motil 26:467-77. 2005..We propose a model in which projectin extends from the Z-band to the lateral borders of the A-band. The PEVK-like domain and a series of Ig domains spanning the intervening I-band may provide the elastic properties of projectin...
- Targeting of cardiac muscle titin fragments to the Z-bands and dense bodies of living muscle and non-muscle cellsJ C Ayoob
Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia 19104 6804, USA
Cell Motil Cytoskeleton 45:67-82. 2000..skeletal myotubes and non-muscle cells lead to the localization of these cardiac z-repeats in the Z-bands of the myofibrils and the dense bodies of the stress fibers...
- Mysteries of muscle contractionWalter Herzog
Faculty of Kinesiology, University of Calgary, AB
J Appl Biomech 24:1-13. 2008..The active component is associated with the cross-bridge kinetics, and the passive component is associated with a calcium-dependent increase in titin stiffness...
- Can all residual force enhancement be explained by sarcomere non-uniformities?David L Morgan
J Physiol 578:613-5; author reply 617-20. 2007
- Troponin C isoform composition determines differences in Sr(2+)-activation characteristics between rat diaphragm fibersBrett O'Connell
School of Biomedical Sciences, Victoria University of Technology, Melbourne, Victoria 8001, Australia
Am J Physiol Cell Physiol 287:C79-87. 2004....
- Ectopic expression and dynamics of TPM1alpha and TPM1kappa in myofibrils of avian myotubesJushuo Wang
Department of Cell and Developmental Biology, SUNY Upstate Medical University, Syracuse, New York 13210, USA
Cell Motil Cytoskeleton 64:767-76. 2007..coupled to chicken TPM1alpha and chicken TPM1kappa and compared their localizations in premyofibrils and mature myofibrils. We used the technique of fluorescence recovery after photobleaching (FRAP) to compare the dynamics of TPM1alpha ..
- Comparison of the tension responses to ramp shortening and lengthening in intact mammalian muscle fibres: crossbridge and non-crossbridge contributionsH Roots
Muscle Contraction Group, Department of Physiology, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, UK
J Muscle Res Cell Motil 28:123-39. 2007....
- Myosin Phosphatase Regulation in Vascular Smooth MuscleHOWARD SURKS; Fiscal Year: 2007..Further preliminary studies show that M-RIP colocalizes with myofibrils in VSMCs, and MRIP itself is a Rho-kinase substrate...
- Role of troponin T isoforms in nemaline myopathyJian Ping Jin; Fiscal Year: 2010..This truncated slow TnT cannot incorporate into myofibrils and is degraded inside the myocyte...
- Role of troponin T isoforms in nemaline myopathyJian Ping Jin; Fiscal Year: 2009..This truncated slow TnT cannot incorporate into myofibrils and is degraded inside the myocyte...
- Proteolytic Regulation of Troponin T & Cardiac FunctionJian Ping Jin; Fiscal Year: 2010..of cTnT selectively removes the N-terminal variable region and keeps the remaining conserved structure in the myofibrils with functional consequences...
- MUSCLE PROTEIN TURNOVER AND AMINO ACID UPTAKE IN SEPSISPer Olof Hasselgren; Fiscal Year: 2003..Because intact myofibrils are not degraded by the proteasome, it is possible that actin and myosin are dissociated from the myofibrils ..
- Role of Troponin T Isoforms in Amish Nemaline MyopathyJian Ping Jin; Fiscal Year: 2007..protein at amino acid 179 We have shown that the truncated slow TnT is degraded and not incorporated into the myofibrils, consistent with the recessive inheritance of the disease The ANM phenotypes include tremors, contractures and ..
- PHYSIOLOGY OF RESPIRATORY MUSCLE MICRO MECHANICSALADIN BORIEK; Fiscal Year: 2004..and longitudinal mechanical properties of the diaphragm, and the strength of the mechanical linkage between myofibrils and the plasma membrane is determined primarily by these proteins; and (3) the mechanical coupling between ..
- Intermediate Filaments & Costamere Structure & FunctionRobert J Bloch; Fiscal Year: 2010Costameres are structures at the surface of striated muscle that align the sarcolemma regularly with nearby myofibrils and transmit contractile force laterally, through the membrane to the extracellular matrix...
- Studies on a Novel RNA that Promotes Heart DevelopmentLARRY LEMANSKI; Fiscal Year: 2006..development, because homozygous embryos (c/c) form hearts that are deficient in tropomyosin, lack organized myofibrils, and fail to beat...
- The Sarcolemma in FSHD and in the myd MouseRobert Bloch; Fiscal Year: 2003..the sarcolemma of FSHD muscle has frequent interruptions in its membrane skeleton, is separated from the nearest myofibrils by a considerable gap, and is organized irregularly, and most closely resembles the sarcolemma of slow twitch ..
- ALTERED MATRIX METALLOPROTEINASES IN HEART FAILUREJEANINE D ARMIENTO; Fiscal Year: 2002..integrity and tensile strength to the myocardium critical to maintaining the alignment of myocytes and myofibrils, and the overall geometry of the left ventricle...
- STUDIES OF A NOVEL RNA APPROACH THAT PROMOTES HEART DEVLARRY LEMANSKI; Fiscal Year: 2001..recessive (c/c) mutant embryos do not beat, as they are deficient in tropomyosin and do not contain organized myofibrils. However, the defect can be corrected by culturing the mutant hearts in the presence of normal anterior endoderm ..
- Role of Troponin T Isoforms in Amish Nemaline MyopathyJian Ping Jin; Fiscal Year: 2003..We have shown that the truncated slow TnT is degraded and not incorporated into the myofibrils, consistent with the recessive inheritance of the disease...
- Role of Troponin T Isoforms in Amish Nemaline MyopathyJian Ping Jin; Fiscal Year: 2006..We have shown that the truncated slow TnT is degraded and not incorporated into the myofibrils, consistent with the recessive inheritance of the disease...
- REGULATION OF MYOTENDINOUS JUNCTION STRUCTUREJames Tidball; Fiscal Year: 1993..Phosphorylation of MTJ structural proteins(s) results in dissociation of myofibrils from the MTJ membrane...
- Costamere Defects in Muscular DystrophiesJAMES ERVASTI; Fiscal Year: 2009..assemblies in striated muscle cells that circumferentially align in register with the Z disk of peripheral myofibrils and physically couple force-generating sarcomeres with the sarcolemma...