myofibrils

Summary

Summary: Highly organized bundles of actin (=ACTINS), MYOSINS, and other proteins in the cytoplasm of skeletal and cardiac muscle cells that contract by a sliding filament mechanism.

Top Publications

  1. pmc Assembly and dynamics of myofibrils
    Joseph W Sanger
    Department of Cell and Developmental Biology, SUNY Upstate Medical University, Syracuse, NY 13210, USA
    J Biomed Biotechnol 2010:858606. 2010
  2. ncbi Protein degradation by the ubiquitin-proteasome pathway in normal and disease states
    Stewart H Lecker
    Nephrology Division, Beth Isreal Deaconess, Harvard Medical School, Boston, Massachusetts, USA
    J Am Soc Nephrol 17:1807-19. 2006
  3. ncbi Transient association of titin and myosin with microtubules in nascent myofibrils directed by the MURF2 RING-finger protein
    Véronique Pizon
    European Molecular Biology Laboratory, Cell Biology Division, Heidelberg, Germany
    J Cell Sci 115:4469-82. 2002
  4. pmc Modulation of energy transfer pathways between mitochondria and myofibrils by changes in performance of perfused heart
    Marko Vendelin
    Laboratory of Systems Biology, Institute of Cybernetics, Tallinn University of Technology, Akadeemia 21, 12618 Tallinn, Estonia
    J Biol Chem 285:37240-50. 2010
  5. pmc Muscle-specific RING finger-1 interacts with titin to regulate sarcomeric M-line and thick filament structure and may have nuclear functions via its interaction with glucocorticoid modulatory element binding protein-1
    Abigail S McElhinny
    Department of Cell Biology and Anatomy, University of Arizona, Tucson, AZ 85724, USA
    J Cell Biol 157:125-36. 2002
  6. pmc History-dependent properties of skeletal muscle myofibrils contracting along the ascending limb of the force-length relationship
    Clara Pun
    Department of Kinesiology and Physical Education, Pine Avenue West 475, Montreal QC H2W1S4, Canada
    Proc Biol Sci 277:475-84. 2010
  7. doi Intercellular and extracellular mechanotransduction in cardiac myocytes
    J Yasha Kresh
    Department of Cardiothoracic Surgery, Drexel University College of Medicine, 245 North 15th Street, MS 111, Philadelphia, PA 19102, USA
    Pflugers Arch 462:75-87. 2011
  8. pmc Ranolazine improves cardiac diastolic dysfunction through modulation of myofilament calcium sensitivity
    Joshua D Lovelock
    Section of Cardiology, University of Illinois at Chicago, 60612, USA
    Circ Res 110:841-50. 2012
  9. pmc Pathogenity of some limb girdle muscular dystrophy mutations can result from reduced anchorage to myofibrils and altered stability of calpain 3
    Natalia Ermolova
    Department of Neurology, David Geffen School of Medicine at University of California, Los Angeles, CA 90095, USA
    Hum Mol Genet 20:3331-45. 2011
  10. pmc SmyD1, a histone methyltransferase, is required for myofibril organization and muscle contraction in zebrafish embryos
    Xungang Tan
    Center of Marine Biotechnology, University of Maryland Biotechnology Institute, Baltimore, MD 21202, USA
    Proc Natl Acad Sci U S A 103:2713-8. 2006

Detail Information

Publications225 found, 100 shown here

  1. pmc Assembly and dynamics of myofibrils
    Joseph W Sanger
    Department of Cell and Developmental Biology, SUNY Upstate Medical University, Syracuse, NY 13210, USA
    J Biomed Biotechnol 2010:858606. 2010
    We review some of the problems in determining how myofibrils may be assembled and just as importantly how this contractile structure may be renewed by sarcomeric proteins moving between the sarcomere and the cytoplasm...
  2. ncbi Protein degradation by the ubiquitin-proteasome pathway in normal and disease states
    Stewart H Lecker
    Nephrology Division, Beth Isreal Deaconess, Harvard Medical School, Boston, Massachusetts, USA
    J Am Soc Nephrol 17:1807-19. 2006
  3. ncbi Transient association of titin and myosin with microtubules in nascent myofibrils directed by the MURF2 RING-finger protein
    Véronique Pizon
    European Molecular Biology Laboratory, Cell Biology Division, Heidelberg, Germany
    J Cell Sci 115:4469-82. 2002
    ..microtubules associate transiently with sarcomeric myosin and later with A-band titin when non-striated myofibrils differentiate into mature sarcomeres...
  4. pmc Modulation of energy transfer pathways between mitochondria and myofibrils by changes in performance of perfused heart
    Marko Vendelin
    Laboratory of Systems Biology, Institute of Cybernetics, Tallinn University of Technology, Akadeemia 21, 12618 Tallinn, Estonia
    J Biol Chem 285:37240-50. 2010
    In the heart, the energy supplied by mitochondria to myofibrils is continuously and finely tuned to the contraction requirement over a wide range of cardiac loads...
  5. pmc Muscle-specific RING finger-1 interacts with titin to regulate sarcomeric M-line and thick filament structure and may have nuclear functions via its interaction with glucocorticoid modulatory element binding protein-1
    Abigail S McElhinny
    Department of Cell Biology and Anatomy, University of Arizona, Tucson, AZ 85724, USA
    J Cell Biol 157:125-36. 2002
    ..The dual interactions of MURF-1 with titin and GMEB-1 may link myofibril signaling pathways (perhaps including titin's kinase domain) with muscle gene expression...
  6. pmc History-dependent properties of skeletal muscle myofibrils contracting along the ascending limb of the force-length relationship
    Clara Pun
    Department of Kinesiology and Physical Education, Pine Avenue West 475, Montreal QC H2W1S4, Canada
    Proc Biol Sci 277:475-84. 2010
    ..Rabbit psoas myofibrils were isolated and attached between two microneedles for force measurements...
  7. doi Intercellular and extracellular mechanotransduction in cardiac myocytes
    J Yasha Kresh
    Department of Cardiothoracic Surgery, Drexel University College of Medicine, 245 North 15th Street, MS 111, Philadelphia, PA 19102, USA
    Pflugers Arch 462:75-87. 2011
    ....
  8. pmc Ranolazine improves cardiac diastolic dysfunction through modulation of myofilament calcium sensitivity
    Joshua D Lovelock
    Section of Cardiology, University of Illinois at Chicago, 60612, USA
    Circ Res 110:841-50. 2012
    ..Oxidative stress has been shown to increase late inward sodium current (I(Na)), reducing the net cytosolic Ca(2+) efflux...
  9. pmc Pathogenity of some limb girdle muscular dystrophy mutations can result from reduced anchorage to myofibrils and altered stability of calpain 3
    Natalia Ermolova
    Department of Neurology, David Geffen School of Medicine at University of California, Los Angeles, CA 90095, USA
    Hum Mol Genet 20:3331-45. 2011
    ..Our data suggest that R448H and D705G mutations affect both CAPN3s anchorage to titin and its stability. These studies reveal a novel mechanism by which mutations that spare enzymatic activity can still lead to calpainopathy...
  10. pmc SmyD1, a histone methyltransferase, is required for myofibril organization and muscle contraction in zebrafish embryos
    Xungang Tan
    Center of Marine Biotechnology, University of Maryland Biotechnology Institute, Baltimore, MD 21202, USA
    Proc Natl Acad Sci U S A 103:2713-8. 2006
    ..Together, these data indicate that SmyD1a and SmyD1b are histone methyltransferases and play a critical role in myofibril organization during myofiber maturation...
  11. ncbi Myofibrillar myopathies: a clinical and myopathological guide
    Rolf Schroder
    Institute of Neuropathology and Department of Neurology, University Hospital Erlangen, Erlangen, Germany
    Brain Pathol 19:483-92. 2009
    ..This review focuses on the clinical and myopathological aspects of genetically defined MFMs, and shall provide a diagnostic guide for this numerically significant group of protein aggregate myopathies...
  12. ncbi Specific interaction of the potassium channel beta-subunit minK with the sarcomeric protein T-cap suggests a T-tubule-myofibril linking system
    T Furukawa
    Department of Physiology, Akita University School of Medicine, Japan
    J Mol Biol 313:775-84. 2001
    ..Striated staining of minK was observed in non-washed, membrane-intact cardiac myofibrils, but not in well-washed, membrane-removed cardiac myofibrils, suggesting that minK localizes on T-tubular ..
  13. ncbi Targeted homozygous deletion of M-band titin in cardiomyocytes prevents sarcomere formation
    Hanny Musa
    Institute for Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds, Leeds, LS2 9JT, UK
    J Cell Sci 119:4322-31. 2006
    ..These results show that integration of the M-band region of titin is required for myosin filament assembly, M-band formation and maturation of the Z-disk...
  14. pmc Knockdown and overexpression of Unc-45b result in defective myofibril organization in skeletal muscles of zebrafish embryos
    Elena P Bernick
    University of Maryland School of Medicine Interdisciplinary Training Program in Muscle Biology, Baltimore, MD 21201, USA
    BMC Cell Biol 11:70. 2010
    ..The effects of unc-45b knockdown on other sarcomeric structures and the phenotype of Unc-45b overexpression, however, are poorly understood in vertebrates...
  15. ncbi Functional consequences of hypertrophic and dilated cardiomyopathy-causing mutations in alpha-tropomyosin
    Audrey N Chang
    Department of Medicine, Mayo Clinic College of Medicine, Mayo Clinic, Rochester, Minnesota 55905, USA
    J Biol Chem 280:34343-9. 2005
    ..The endogenous Tm-Tn complex was selectively removed from myofibrils and replaced with recombinant wild-type or mutant proteins...
  16. doi Genesis of muscle fiber-type diversity during mouse embryogenesis relies on Six1 and Six4 gene expression
    Anne Françoise Richard
    Institut Cochin, Universite Paris Descartes, Sorbonne Paris Cité, CNRS UMR 8104, Paris, France
    Dev Biol 359:303-20. 2011
    ..Six genes thus behave as essential global regulators of muscle gene expression, as well as a central switch to drive the skeletal muscle fast phenotype during fetal development...
  17. pmc Muscle wasting and interleukin-6-induced atrogin-I expression in the cachectic Apc ( Min/+ ) mouse
    Kristen A Baltgalvis
    Exercise Science Department, University of South Carolina, Columbia, USA
    Pflugers Arch 457:989-1001. 2009
    ..These data suggest that high circulating IL-6 levels induce type IIB fiber CSA loss in Apc ( Min/+ ) mice, and circulating IL-6 is sufficient to regulate Atrogin-I gene expression in cachectic mice...
  18. ncbi Interaction between PEVK-titin and actin filaments: origin of a viscous force component in cardiac myofibrils
    M Kulke
    Institute of Physiology, University of Heidelberg, Heidelberg, Germany
    Circ Res 89:874-81. 2001
    ..To probe the interaction in a more physiological environment, nonactivated cardiac myofibrils were stretched quickly, and force was measured during the subsequent hold period...
  19. doi Protein kinase G modulates human myocardial passive stiffness by phosphorylation of the titin springs
    Martina Kruger
    Physiology and Biophysics Unit, University of Muenster, Germany
    Circ Res 104:87-94. 2009
    ..determine whether PKG can phosphorylate titin and affect titin-based stiffness in skinned myofibers and isolated myofibrils. PKG in the presence of 8-pCPT-cGMP (cGMP) phosphorylated the 2 main cardiac titin isoforms, N2BA and N2B, in ..
  20. ncbi Metabolic consequences of functional complexes of mitochondria, myofibrils and sarcoplasmic reticulum in muscle cells
    T Andrienko
    Laboratory of Fundamental and Applied Bioenergetics, INSERM E0221, Joseph Fourier University, Grenoble, France
    J Exp Biol 206:2059-72. 2003
    ..Inside these complexes, mitochondrial functional state is controlled by channeling of ADP, mostly via energy- and phosphoryl-transfer networks, and apparently depends on the state of sarcomere structures...
  21. ncbi Exercise induces a cardiac mitochondrial phenotype that resists apoptotic stimuli
    Andreas N Kavazis
    Department of Applied Physiology and Kinesiology, University of Florida, Gainesville, FL 32611, USA
    Am J Physiol Heart Circ Physiol 294:H928-35. 2008
    ..Furthermore, these results are consistent with the concept that exercise-induced mitochondrial adaptations contribute to exercise-induced cardioprotection...
  22. pmc Diastolic dysfunction in familial hypertrophic cardiomyopathy transgenic model mice
    Theodore P Abraham
    Division of Cardiology, Johns Hopkins University, Baltimore, MD, USA
    Cardiovasc Res 82:84-92. 2009
    ....
  23. pmc Binding of an ankyrin-1 isoform to obscurin suggests a molecular link between the sarcoplasmic reticulum and myofibrils in striated muscles
    Paola Bagnato
    Molecular Medicine Section, Department of Neuroscience, University of Siena, 53100 Siena, Italy
    J Cell Biol 160:245-53. 2003
    ..The mechanisms that mediate the structural organization of the sarcoplasmic reticulum with respect to the myofibrils are, however, not known. We report here that ank1...
  24. pmc Paralysis and delayed Z-disc formation in the Xenopus tropicalis unc45b mutant dicky ticker
    Timothy J Geach
    Division of Developmental Biology, MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK
    BMC Dev Biol 10:75. 2010
    ..Chaperone proteins specific to sarcomeric myosins have been shown to be necessary in zebrafish and invertebrates for proper muscle assembly and function...
  25. ncbi Functional consequences of the mutations in human cardiac troponin I gene found in familial hypertrophic cardiomyopathy
    F Takahashi-Yanaga
    Department of Clinical Pharmacology, Graduate School of Medical Sciences, Kyushu University, Fukuoka 812 8582, Japan
    J Mol Cell Cardiol 33:2095-107. 2001
    ..Exchanging the human cardiac troponin into isolated cardiac myofibrils or skinned cardiac muscle fibers showed that the mutations R145G, R145Q, R162W, DeltaK183 and K206Q induced a ..
  26. pmc Self-organization of muscle cell structure and function
    Anna Grosberg
    Disease Biophysics Group, Wyss Institute for Biologically Inspired Engineering, School of Engineering and Applied Sciences, Harvard University, Cambridge, Massachusetts, United States of America
    PLoS Comput Biol 7:e1001088. 2011
    ..fibers, premyofibrils and focal adhesions, as well as cooperative alignment and parallel bundling of nascent myofibrils. Our results suggest that a hierarchy of mechanisms regulate the self-organization of the contractile ..
  27. pmc Properties of easily releasable myofilaments: are they the first step in myofibrillar protein turnover?
    Girija Neti
    Muscle Biology Group, 626 Shantz, University of Arizona, Tucson, AZ 85721, USA
    Am J Physiol Cell Physiol 296:C1383-90. 2009
    ..It has been proposed that the calpains could remove the outer layer of filaments from myofibrils as a first step in myofibrillar protein turnover...
  28. ncbi The mechanical behavior of individual sarcomeres of myofibrils isolated from rabbit psoas muscle
    Ivan Pavlov
    Department of Kinesiology and Physical Education, McGill University, Montreal, Quebec, Canada, H2W1S4
    Am J Physiol Cell Physiol 297:C1211-9. 2009
    ..Single myofibrils from rabbit psoas were transferred into a temperature-controlled (22 degrees C or 15 degrees C) experimental ..
  29. ncbi Tension generation and relaxation in single myofibrils from human atrial and ventricular myocardium
    Nicoletta Piroddi
    Centro Interuniversitario di Medicina Molecolare e Biofisica Applicata, Florence, Italy
    Pflugers Arch 454:63-73. 2007
    Fast solution switching techniques in single myofibrils offer the opportunity to dissect and directly examine the sarcomeric mechanisms responsible for force generation and relaxation...
  30. ncbi Homogeneity of cardiac contraction despite physiological asynchrony of depolarization: a model study
    R C P Kerckhoffs
    Department of Biomedical Engineering, Eindhoven University of Technology, Whoog 4 105, P O Box 513, 5600 Eindhoven, The Netherlands
    Ann Biomed Eng 31:536-47. 2003
    ..The present finding may indicate that electromechanical delay times are heterogeneously distributed, such that a contraction in a normal heart is more synchronous than depolarization...
  31. pmc Pre-power stroke cross bridges contribute to force during stretch of skeletal muscle myofibrils
    Dilson E Rassier
    Department of Kinesiology and Physical Education Faculty of Education, McGill University, 475 Pine Avenue West, Montreal, Quebec, Canada
    Proc Biol Sci 275:2577-86. 2008
    ..b>Myofibrils were activated in sarcomere lengths (SLs) between 2.2 and 2...
  32. pmc Dynamic behaviour of half-sarcomeres during and after stretch in activated rabbit psoas myofibrils: sarcomere asymmetry but no 'sarcomere popping'
    I A Telley
    Laboratory for Biomechanics, ETH Zurich, ETH Honggerberg, HCI E 357 1, CH 8093 Zurich, Switzerland
    J Physiol 573:173-85. 2006
    ..changes of individual half-sarcomeres during and after stretch in actively contracting, single rabbit psoas myofibrils containing 10-30 sarcomeres...
  33. pmc The UNC-45 chaperone is critical for establishing myosin-based myofibrillar organization and cardiac contractility in the Drosophila heart model
    Girish C Melkani
    Department of Biology, Molecular Biology Institute and Heart Institute, San Diego State University, San Diego, California, United States of America
    PLoS ONE 6:e22579. 2011
    ..Structural analysis showed reduced myofibrils, myofibrillar disarray, and greatly decreased cardiac myosin accumulation...
  34. pmc Residual force enhancement in myofibrils and sarcomeres
    V Joumaa
    University of Calgary, 2500 University Drive NW, Calgary, Alberta, Canada T2N 1N4
    Proc Biol Sci 275:1411-9. 2008
    ..Here, we studied force enhancement for the first time in isolated myofibrils (n=18) that, owing to the strict in series arrangement, allowed for evaluation of this property in individual ..
  35. pmc Cardiac myofibrillogenesis inside intact embryonic hearts
    Aiping Du
    Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 6058, USA
    Dev Biol 318:236-46. 2008
    How proteins assemble into sarcomeric arrays to form myofibrils is controversial...
  36. doi Differential involvement of sarcomeric proteins in myofibrillar myopathies: a morphological and immunohistochemical study
    Kristl G Claeys
    Institut de Myologie, Groupe Hospitalier Pitie Salpetriere, Paris, France
    Acta Neuropathol 117:293-307. 2009
    ....
  37. ncbi Lys184 deletion in troponin I impairs relaxation kinetics and induces hypercontractility in murine cardiac myofibrils
    Bogdan Iorga
    Institute of Vegetative Physiology, University of Cologne, Robert Koch Strasse 39, Cologne, Germany
    Cardiovasc Res 77:676-86. 2008
    ....
  38. ncbi Functional properties of the titin/connectin-associated proteins, the muscle-specific RING finger proteins (MURFs), in striated muscle
    Carol C Gregorio
    Department of Cell Biology and Anatomy, University of Arizona, Tucson, AZ 85724, USA
    J Muscle Res Cell Motil 26:389-400. 2005
    ..is dependent upon the proper alignment and coordinated activities of several cytoskeletal networks including myofibrils, microtubules, and intermediate filaments...
  39. ncbi Structural basis for activation of the titin kinase domain during myofibrillogenesis
    O Mayans
    European Molecular Biology Laboratory, Hamburg Outstation, Germany
    Nature 395:863-9. 1998
    ..Titin kinase phosphorylates the muscle protein telethonin in early differentiating myocytes, indicating that this kinase may act in myofibrillogenesis...
  40. ncbi Myofibrillogenesis in the first cardiomyocytes formed from isolated quail precardiac mesoderm
    Aiping Du
    Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 6058, USA
    Dev Biol 257:382-94. 2003
    De novo assembly of myofibrils was investigated in explants of precardiac mesoderm from quail embryos to address a controversy about different models of myofibrillogenesis...
  41. doi Distinct mechanisms for diastolic dysfunction in diabetes mellitus and chronic pressure-overload
    Ines Falcao-Pires
    Department of Physiology and Cardiothoracic Surgery, Cardiovascular R and D Unit, Faculty of Medicine, University of Porto, Alameda Professor Hernani Monteiro, 4200 319 Porto, Portugal
    Basic Res Cardiol 106:801-14. 2011
    ..The association of these damages accelerates the progression of diastolic heart failure progression in diabetic-banded animals...
  42. pmc Myofibrillar myopathies
    Duygu Selcen
    Department of Neurology, Division of Child Neurology and Neuromuscular Disease Research Laboratory, Mayo Clinic College of Medicine, Mayo Clinic, Rochester, MN, USA
    Neuromuscul Disord 21:161-71. 2011
    ..However, in the majority of the myofibrillar myopathy patients the disease gene awaits discovery...
  43. doi Insights into the kinetics of Ca2+-regulated contraction and relaxation from myofibril studies
    Robert Stehle
    Institute of Physiology, University of Cologne, Robert Koch Str 39, 50931 Cologne, Germany
    Pflugers Arch 458:337-57. 2009
    ..techniques that analyse the chemical and mechanical kinetics of small components of a muscle fibre, subcellular myofibrils isolated from skeletal and cardiac muscle...
  44. ncbi Why choose myofibrils to study muscle myosin ATPase?
    Corinne Lionne
    UMR 5121, CNRS Université Montpellier I, Institut de Biologie, 4 boulevard Henri IV CS89508, 34960 Montpellier 2, France
    J Muscle Res Cell Motil 24:139-48. 2003
    ..In the presence of calcium, myofibrillar ATPase is activated and myofibrils either shorten with no external load (native myofibrils) or contract isometrically (cross-linked myofibrils)...
  45. pmc Myofibrillogenesis in the developing zebrafish heart: A functional study of tnnt2
    Wei Huang
    Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine, Rochester, MN 55905, USA
    Dev Biol 331:237-49. 2009
    ..In summary, our data indicates that zebrafish are a valuable in vivo model for studying both myofibrillogenesis and sarcomere-based cardiac diseases...
  46. pmc Actin-titin interaction in cardiac myofibrils: probing a physiological role
    W A Linke
    Institute of Physiology II, University of Heidelberg, Germany
    Biophys J 73:905-19. 1997
    The high stiffness of relaxed cardiac myofibrils is explainable mainly by the expression of a short-length titin (connectin), the giant elastic protein of the vertebrate myofibrillar cytoskeleton...
  47. ncbi Form follows function: how muscle shape is regulated by work
    B Russell
    Department of Physiology and Biophysics, College of Medicine, University of Illinois at Chicago, Chicago, Illinois 60612, USA
    J Appl Physiol (1985) 88:1127-32. 2000
    ..of cell shape and the composition and aggregation of contractile proteins into filaments, the sarcomere, and myofibrils. We examine how mechanical activity regulates the turnover and exchange of contraction proteins...
  48. pmc A new 185,000-dalton skeletal muscle protein detected by monoclonal antibodies
    B K Grove
    J Cell Biol 98:518-24. 1984
    ..antibodies (mAbs) against a 165-kd fraction, we also obtained mAbs that bound to the M line of isolated myofibrils as detected by indirect immunofluorescence, but recognized a protein band of 185 kd in immunoblotting ..
  49. ncbi MURF-1 and MURF-2 target a specific subset of myofibrillar proteins redundantly: towards understanding MURF-dependent muscle ubiquitination
    Stephanie H Witt
    Institut fur Anasthesiologie und Operative Intensivmedizin, Universitatsklinikum Mannheim, Mannheim 68167, Germany
    J Mol Biol 350:713-22. 2005
    ..These data raise the possibility that MURF-1 may coordinately regulate the energy metabolism of mitochondrial and cytoplasmic compartments...
  50. ncbi AMPK activation stimulates myofibrillar protein degradation and expression of atrophy-related ubiquitin ligases by increasing FOXO transcription factors in C2C12 myotubes
    Kazuki Nakashima
    Molecular Nutrition Research Team, National Institute of Livestock and Grassland Science, Ikenoda, Tsukuba, Japan
    Biosci Biotechnol Biochem 71:1650-6. 2007
    ..These results indicate that activation of AMPK stimulates myofibrillar protein degradation through the expression of atrogin-1/MAFbx and MuRF1 by increasing FOXO transcription factors in skeletal muscles...
  51. pmc Desmin is essential for the tensile strength and integrity of myofibrils but not for myogenic commitment, differentiation, and fusion of skeletal muscle
    Z Li
    Station Centrale de Microscopie Electronique, Institut Pasteur, Paris, France
    J Cell Biol 139:129-44. 1997
    ..The results presented here show that desmin is essential to maintain the structural integrity of highly solicited skeletal muscle...
  52. pmc Aberrant myofibril assembly in tropomodulin1 null mice leads to aborted heart development and embryonic lethality
    Kimberly L Fritz-Six
    Department of Cell Biology, The Scripps Research Institute, 10550 N Torrey Pines Road, La Jolla, CA 92037, USA
    J Cell Biol 163:1033-44. 2003
    ..5. Confocal microscopy of hearts of E8-8.5 Tmod1 null embryos revealed structures resembling nascent myofibrils with continuous F-actin staining and periodic dots of alpha-actinin, indicating that I-Z-I complexes assembled ..
  53. ncbi Molecular diversity of myofibrillar proteins: gene regulation and functional significance
    S Schiaffino
    Department of Biomedical Sciences, University of Padova, Padua, Italy
    Physiol Rev 76:371-423. 1996
    ..Both myosin and troponin isoforms contribute to the differences in the resistance to fatigue of muscle fibers...
  54. ncbi Obscurin is required for the lateral alignment of striated myofibrils in zebrafish
    Maide O Raeker
    Department of Pediatrics and Communicable Diseases, University of Michigan, Ann Arbor, Michigan 48109, USA
    Dev Dyn 235:2018-29. 2006
    ..independent morpholino antisense oligonucleotides resulted in diminished numbers and marked disarray of skeletal myofibrils, impaired lateral alignment of adjacent myofibrils, disorganization of the sarcoplasmic reticulum, somite ..
  55. ncbi Glucose and palmitic acid induce degeneration of myofibrils and modulate apoptosis in rat adult cardiomyocytes
    D Dyntar
    Division of Endocrinology and Diabetes, University Hospital, Zurich, Switzerland
    Diabetes 50:2105-13. 2001
    ..C2-dihydroceramide enhanced apoptosis of cardiomyocytes by 50%, accompanied by detrimental effects on the myofibrils. The palmitic acid-induced effects were impaired by fumonisin B1, an inhibitor of ceramide synthase...
  56. pmc Mechanical stress-strain sensors embedded in cardiac cytoskeleton: Z disk, titin, and associated structures
    Masahiko Hoshijima
    Department of Medicine, University of California, San Diego, La Jolla, CA 92093 0734, USA
    Am J Physiol Heart Circ Physiol 290:H1313-25. 2006
    ..This review summarizes current knowledge of this rapidly developing area with focus on how the heart adjusts physiological remodeling process to meet with mechanical demands and how this process fails in cardiac pathologies...
  57. ncbi The M-band: an elastic web that crosslinks thick filaments in the center of the sarcomere
    Irina Agarkova
    Institute of Cell Biology, Swiss Federal Institute of Technology, ETH Honggerberg, CH 8093 Zurich
    Trends Cell Biol 15:477-85. 2005
    ..We propose that the M-band is in fact a dynamic structure that monitors the stress appearing in the thick filament lattice during contraction and quickly reorganizes to meet new physiological requirements...
  58. pmc Nebulin-deficient mice exhibit shorter thin filament lengths and reduced contractile function in skeletal muscle
    Marie Louise Bang
    Department of Medicine, Veterans Affairs Medical Center, University of California, San Diego, La Jolla, CA 92093, USA
    J Cell Biol 173:905-16. 2006
    ..The functional importance of nebulin in skeletal muscle function was revealed by isometric contractility assays, which demonstrated a dramatic reduction in force production in nebulin-deficient skeletal muscle...
  59. pmc Myofibrillogenesis in skeletal muscle cells in zebrafish
    Joseph W Sanger
    Department of Cell and Developmental Biology, SUNY Upstate Medical University, Syracuse, New York, USA
    Cell Motil Cytoskeleton 66:556-66. 2009
    ..model of myofibrillogenesis, based on observations in cultured avian muscle cells, proposes that mature myofibrils are preceded by two intermediary structures: premyofibrils and nascent myofibrils...
  60. pmc Myosin transducer mutations differentially affect motor function, myofibril structure, and the performance of skeletal and cardiac muscles
    Anthony Cammarato
    Department of Biology and Heart Institute, San Diego State University, San Diego, CA 92182 4614, USA
    Mol Biol Cell 19:553-62. 2008
    ..Our data suggest Drosophila is a valuable system for identifying and modeling mutations analogous to those associated with specific human muscle disorders...
  61. ncbi Myofibrillar myopathy: clinical, morphological and genetic studies in 63 patients
    Duygu Selcen
    Department of Neurology, Neuromuscular Research Laboratory, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA
    Brain 127:439-51. 2004
    ..Further advances in defining the molecular causes of MFM will probably come from linkage studies of informative kinships or from systematic search for mutations in proteins participating in the intricate network supporting the Z-disk...
  62. pmc Myofibrillar architecture in engineered cardiac myocytes
    Kevin Kit Parker
    Disease Biophysics Group, School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA kkparker seas harvard edu
    Circ Res 103:340-2. 2008
    ..These data suggest that physical constrainment of muscle cells by extracellular matrix may be an important regulator of myofibrillar organization...
  63. doi Sarcomere alignment is regulated by myocyte shape
    Mark Anthony Bray
    Disease Biophysics Group, School of Engineering and Applied Sciences, Harvard University, Cambridge, Massachusetts, USA
    Cell Motil Cytoskeleton 65:641-51. 2008
    ..Furthermore, geometric boundaries such as corners induce localized myofibrillar anisotropy that becomes global as the myocyte aspect ratio increases...
  64. ncbi De novo myofibrillogenesis in C2C12 cells: evidence for the independent assembly of M bands and Z disks
    Aikaterini Kontrogianni-Konstantopoulos
    Dept of Physiology, School of Medicine, Univ of Maryland at Baltimore, 685 W Baltimore St, Baltimore, MD 21201, USA
    Am J Physiol Cell Physiol 290:C626-37. 2006
    ....
  65. pmc Titin: major myofibrillar components of striated muscle
    K Wang
    Proc Natl Acad Sci U S A 76:3698-702. 1979
    ..proteins, we purified a combination of two uncommonly large proteins, designated as titin, from chicken breast myofibrils. Chemical and immunological studies indicated that titin is distinct from myosin, actin, and filamin...
  66. ncbi How to build a myofibril
    Joseph W Sanger
    Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 6058, USA
    J Muscle Res Cell Motil 26:343-54. 2005
    ..The models hypothesize: (a) stress fiber-like structures as templates for the assembly of myofibrils, (b) assembly in which the actin filaments and Z-bands form subunits independently from A-band subunits, with ..
  67. ncbi Concentration-dependent suppressive effect of shrimp head protein hydrolysate on dehydration-induced denaturation of lizardfish myofibrils
    Yaowalux Ruttanapornvareesakul
    Graduate School of Science and Technology, Nagasaki University, Bunkyo machi 1 14, Nagasaki 852 8521, Japan
    Bioresour Technol 97:762-9. 2006
    ..The SHPH was used as a natural food preservative by adding to lizardfish myofibrils at concentrations ranging from 2.5% to 10%...
  68. ncbi Tyrosinase-aided protein cross-linking: effects on gel formation of chicken breast myofibrils and texture and water-holding of chicken breast meat homogenate gels
    R Lantto
    VTT, P O Box 1000, 02044 VTT, Finland
    J Agric Food Chem 55:1248-55. 2007
    ..Microstructural characterization showed that a collagen network was formed in the presence of tyrosinase...
  69. ncbi Extraction and replacement of the tropomyosin-troponin complex in isolated myofibrils
    Beatrice Scellini
    Dipartmento di Scienze Fisiologiche, Universita di Firenze, Firenze, Italy
    Adv Exp Med Biol 682:163-74. 2010
    ..Here we describe the method we recently developed to replace Tm-Tn of skeletal and cardiac myofibrils from animals and humans to generate an experimental model of homogeneous Tm composition and giving the ..
  70. ncbi Contractile characteristics of sarcomeres arranged in series or mechanically isolated from myofibrils
    Dilson E Rassier
    Department of Kinesiology and Physical Education, McGill University, Montreal, QC, Canada
    Adv Exp Med Biol 682:123-40. 2010
    ..This chapter presents two sets of experiments performed with (1) myofibrils, in which individual sarcomeres in series can be evaluated during contractions, and (2) mechanically isolated ..
  71. pmc Cardiac myocyte remodeling mediated by N-cadherin-dependent mechanosensing
    Anant Chopra
    Department of Biomedical Engineering, Drexel University, Philadelphia, PA 19102, USA
    Am J Physiol Heart Circ Physiol 300:H1252-66. 2011
    ..These results indicate that cell-to-cell-mediated force perception and transmission are involved in the organization and development of cardiac structure and function...
  72. pmc Second harmonic generation microscopy probes different states of motor protein interaction in myofibrils
    Sebastian Schurmann
    Medical Biophysics, Institute of Physiology and Pathophysiology, Heidelberg University, Heidelberg, Germany
    Biophys J 99:1842-51. 2010
    ..In summary, different actomyosin states can be visualized noninvasively with SHG microscopy. Specifically, SHG even allows us to distinguish different actin-bound states of myosin II using γ as a parameter...
  73. ncbi Immunocytochemical studies using a monoclonal antibody to bovine cardiac titin on intact and extracted myofibrils
    S M Wang
    J Muscle Res Cell Motil 6:293-312. 1985
    ..The antibody recognizes a common antigenic site in striated muscles of several species. In relaxed myofibrils, specific staining at the A-I junction resulted in a doublet of fluorescent bands within a sarcomere...
  74. pmc Interactions between connected half-sarcomeres produce emergent mechanical behavior in a mathematical model of muscle
    Kenneth S Campbell
    Department of Physiology and Center for Muscle Biology, University of Kentucky, Lexington, Kentucky, USA
    PLoS Comput Biol 5:e1000560. 2009
    ..rabbit psoas fiber could be reproduced with a framework consisting of 300 half-sarcomeres arranged in 6 parallel myofibrils without requiring titin filaments to stiffen in activating solutions...
  75. doi Single molecule detection approach to muscle study: kinetics of a single cross-bridge during contraction of muscle
    Julian Borejdo
    Department of Molecular Biology and Immunology, Center for Commercialization of Fluorescence Technologies, University of North Texas Health Science Center, Fort Worth, TX, USA
    Methods Mol Biol 875:311-34. 2012
    ..of cross-bridge attachment and dissociation are significantly different in isometrically contracting cardiac myofibrils from right ventricle of WT and Tg-D166V mice...
  76. pmc The rates of Ca2+ dissociation and cross-bridge detachment from ventricular myofibrils as reported by a fluorescent cardiac troponin C
    Sean C Little
    Department of Physiology and Cell Biology, College of Medicine, The Ohio State University, Columbus, Ohio 43210, USA
    J Biol Chem 287:27930-40. 2012
    ..dissociation from TnC and the rate of cross-bridge detachment from several different species of ventricular myofibrils. The fluorescently labeled TnC was sensitive to both Ca(2+) dissociation and cross-bridge detachment at low Ca(2+..
  77. pmc Myofiber angle distributions in the ovine left ventricle do not conform to computationally optimized predictions
    Daniel B Ennis
    Department of Cardiothoracic Surgery, 300 Pasteur Drive, Falk CVRB, Stanford University, Stanford, CA 94305 5488, USA
    J Biomech 41:3219-24. 2008
    ..To test the predictive capability of the current computational model would we propose using an ovine specific LV geometry and comparing the computed myofiber orientations to those we report herein...
  78. pmc Modulation by substrate concentration of maximal shortening velocity and isometric force in single myofibrils from frog and rabbit fast skeletal muscle
    C Tesi
    Dipartimento di Scienze Fisiologiche, Universita degli Studi di Firenze, Viale G B Morgagni 63, I 50134 Firenze, Italy
    J Physiol 516:847-53. 1999
    ..on maximal force and shortening velocity have been studied at 5 C in single and thin bundles of striated muscle myofibrils. The minute diameters of the preparations promote rapid diffusional equilibrium between the bathing medium and ..
  79. ncbi Effects of calmodulin and okadaic acid on myofibrillar Ca2+ sensitivity in cardiac myocytes
    Nobuhiro Suematsu
    Department of Cardiovascular Medicine, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan
    Basic Res Cardiol 97:137-44. 2002
    ..05). These results suggest that MLC2 phosphorylation plays a partial role in the regulation of myofibrillar Ca2+ sensitivity in cardiac muscle...
  80. ncbi Susceptibility of isolated myofibrils to in vitro glutathionylation: Potential relevance to muscle functions
    Chiara Passarelli
    Children s Hospital and Research Institute Bambino Gesu, Rome, Italy
    Cytoskeleton (Hoboken) 67:81-9. 2010
    In this study we investigated the molecular mechanism of glutathionylation on isolated human cardiac myofibrils using several pro-glutathionylating agents...
  81. pmc Myofibrillar myopathies
    Duygu Selcen
    Department of Neurology, Division of Child Neurology and Neuromuscular Research Laboratory, Mayo Clinic, College of Medicine Rochester, Minnesota, USA
    Curr Opin Neurol 21:585-9. 2008
    ..The aim of this communication is to provide an up-to-date overview of myofibrillar myopathies...
  82. ncbi Extralysosomal protein degradation in myofibrillar myopathies
    Montse Olive
    Institut de Neuropatologia, IDIBELL Hospital Universitari de Bellvitge, Hospitalet de Llobregat, Barcelona, Spain
    Brain Pathol 19:507-15. 2009
    ..a group of heterogeneous muscle disorders morphologically defined by the presence of foci of dissolution of the myofibrils, accumulation of the products of myofibrillar degradation and ectopic expression of multiple proteins...
  83. ncbi Late-stage alterations in myofibrillar contractile function in a transgenic mouse model of dilated cardiomyopathy (Tgalphaq*44)
    István Ferenc Edes
    Division of Clinical Physiology, Institute of Cardiology University of Debrecen, Medical and Health Science Center, Faculty of Medicine Móricz Zs krt 22 H 4032 Debrecen, Hungary
    J Mol Cell Cardiol 45:363-72. 2008
    ..In conclusion, our data suggested that an initial decrease of PKA signaling and subsequent changes in myofilament protein expression may contribute to the development of dilated cardiomyopathy in Tgalphaq*44 hearts...
  84. pmc Effects of the mutation R145G in human cardiac troponin I on the kinetics of the contraction-relaxation cycle in isolated cardiac myofibrils
    M Kruger
    Department of Vegetative Physiology, University of Cologne, Koeln, Germany
    J Physiol 564:347-57. 2005
    ..of the mutation hcTnI(R145G) on crossbridge kinetics, force kinetics were analysed in murine cardiac myofibrils carrying either the mutant or the wild-type protein...
  85. ncbi Ultrastructural organization of the transverse tubules and the sarcoplasmic reticulum in a fish sound-producing muscle
    Suechika Suzuki
    Department of Physiology, School of Medicine, Teikyo University, Itabashi ku, Tokyo 173 8605, Japan
    J Electron Microsc (Tokyo) 52:337-47. 2003
    ..These results are discussed in connection with the mechanism, by which the mechanical activity of the SBM muscle is neurally controlled...
  86. doi Effects of chronic atrial fibrillation on active and passive force generation in human atrial myofibrils
    Alexandra Belus
    Center of Molecular Medicine, Department of Physiology, University of Florence, Italy
    Circ Res 107:144-52. 2010
    ..Chronic atrial fibrillation (cAF) is associated with atrial contractile dysfunction. Sarcomere remodeling may contribute to this contractile disorder...
  87. ncbi Assembly and remodelling of myofibrils and intercalated discs in cultured neonatal rat heart cells
    B T Atherton
    Department of Cell Biology and Anatomy, Northwestern University Medical School, Chicago, Illinois 60611
    J Cell Sci 86:233-48. 1986
    The reorganization of myofibrils and the re-formation of intercalated discs was examined in neonatal rat cardiac muscle cells during the first 72 h of culture...
  88. ncbi Expression of protein kinase C isoforms and interleukin-1beta in myofibrillar myopathy
    G Vattemi
    Department of Neurological Sciences and Vision, Section of Clinical Neurology, Clinica Neurologica, Policlinico G B Rossi, Verona, Italy
    Neurology 62:1778-82. 2004
    ..The pathogenesis of this myopathy is not well understood. The morphologic hallmark is myofibrillar destruction with abnormal expression of numerous proteins, most consistently of desmin...
  89. ncbi Autophagy is required to maintain muscle mass
    Eva Masiero
    Dulbecco Telethon Institute, 35129 Padova, Italy
    Cell Metab 10:507-15. 2009
    ..Our results suggest that inhibition/alteration of autophagy can contribute to myofiber degeneration and weakness in muscle disorders characterized by accumulation of abnormal mitochondria and inclusions...
  90. ncbi Localization of the human 64kD autoantigen D1 to myofibrils in a subset of extraocular muscle fibers
    C A Conley
    Department of Cell Biology, MB24, The Scripps Research Institute 10550 N Torrey Pines Rd, CA 92037, La Jolla, USA
    Curr Eye Res 19:313-22. 1999
    ..To evaluate the tissue-specific expression pattern of the 64kD human autoantigen D1, a tropomodulin-related protein that may be involved in thyroid-associated ophthalmopathy...
  91. ncbi Ultrastructural and biochemical localization of N-RAP at the interface between myofibrils and intercalated disks in the mouse heart
    J Q Zhang
    Laboratory of Muscle Biology, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    Biochemistry 40:14898-906. 2001
    ..addition, immunofluorescence shows that N-RAP remains bound at the ends of isolated, detergent-treated cardiac myofibrils. These results demonstrate that N-RAP remains tightly bound to myofibrils and fasciae adherentes during ..
  92. pmc Monolayers of silver nanoparticles decrease photobleaching: application to muscle myofibrils
    P Muthu
    Department of Molecular Biology and Immunology, University of North Texas Health Science Center, Fort Worth, Texas 76107 2699, USA
    Biophys J 95:3429-38. 2008
    ..Skeletal muscle myofibrils were used as an example, because they contain submicron structures conveniently used to define optical ..
  93. ncbi Isometric force kinetics upon rapid activation and relaxation of mouse, guinea pig and human heart muscle studied on the subcellular myofibrillar level
    Robert Stehle
    Institute of Physiology, Universitat zu Koln, Robert Koch Str 39, 50931 Koln, Germany
    Basic Res Cardiol 97:I127-35. 2002
    ..of force development and relaxation upon rapid application and removal of Ca2+ was measured in bundles of few myofibrils isolated from triton X-100 skinned left ventricular trabeculae of mice (M), guinea pigs (G) and humans (H)...
  94. ncbi Evidence that phosphate release is the rate-limiting step on the overall ATPase of psoas myofibrils prevented from shortening by chemical cross-linking
    Corinne Lionne
    INSERM U128, 1919 Route de Mende, 34293 Montpellier Cedex 5, France
    Biochemistry 41:13297-308. 2002
    ..We have put this idea to the test with myofibrils as a model because with these both mechanical and chemical kinetic measurements are possible...
  95. pmc A mutation in the dimerization domain of filamin c causes a novel type of autosomal dominant myofibrillar myopathy
    Matthias Vorgerd
    Department of Neurology, Neuromuscular Center Ruhrgebiet, Ruhr University Bochum, Bochum, Germany
    Am J Hum Genet 77:297-304. 2005
    ..As a consequence of this malfunction, the muscle fibers of our patients display massive cytoplasmic aggregates containing filamin c and several Z-disk-associated and sarcolemmal proteins...
  96. pmc Coordinated collagen and muscle protein synthesis in human patella tendon and quadriceps muscle after exercise
    Benjamin F Miller
    Institute of Sports Medicine, Copenhagen, Bispebjerg Hospital, Denmark
    J Physiol 567:1021-33. 2005
    ..The similar time course of changes of protein synthetic rates in different cell types supports the idea of coordinated musculotendinous adaptation...
  97. pmc Tropomodulin1 is required in the heart but not the yolk sac for mouse embryonic development
    Caroline R McKeown
    The Scripps Research Institute, Department of Cell Biology, La Jolla, CA 92037, USA
    Circ Res 103:1241-8. 2008
    Tropomodulin (Tmod)1 caps the pointed ends of actin filaments in sarcomeres of striated muscle myofibrils and in the erythrocyte membrane skeleton...
  98. pmc Exercise training induces a cardioprotective phenotype and alterations in cardiac subsarcolemmal and intermyofibrillar mitochondrial proteins
    Andreas N Kavazis
    Applied Physiology and Kinesiology, Proteomics Division, University of Florida, Gainesville, FL 32611, USA
    Am J Physiol Heart Circ Physiol 297:H144-52. 2009
    ..Importantly, based on our findings, pharmacological or other interventions could be used to develop a strategy of protecting the myocardium during an ischemic attack...
  99. pmc Nemaline myopathy with minicores caused by mutation of the CFL2 gene encoding the skeletal muscle actin-binding protein, cofilin-2
    Pankaj B Agrawal
    Genomics Program, the Divisions of Genetics and Neonatology, Children s Hospital Boston, Boston, MA, 02115, USA
    Am J Hum Genet 80:162-7. 2007
    ....
  100. pmc Myofibrillar calcium sensitivity of isometric tension is increased in human dilated cardiomyopathies: role of altered beta-adrenergically mediated protein phosphorylation
    M R Wolff
    Department of Medicine, University of Wisconsin, Madison 53792, USA
    J Clin Invest 98:167-76. 1996
    ....
  101. pmc Half-sarcomere dynamics in myofibrils during activation and relaxation studied by tracking fluorescent markers
    Ivo A Telley
    Laboratory for Biomechanics, ETH Zurich Honggerberg, 8093 Zurich, Switzerland
    Biophys J 90:514-30. 2006
    To study the dynamics of individual half-sarcomeres in striated muscle contraction, myofibrils prepared from rabbit psoas muscle and left ventricles of guinea pig were immunostained with two conjugated antibody complexes consisting of a ..

Research Grants72

  1. Myosin Phosphatase Regulation in Vascular Smooth Muscle
    HOWARD SURKS; Fiscal Year: 2007
    ..Further preliminary studies show that M-RIP colocalizes with myofibrils in VSMCs, and MRIP itself is a Rho-kinase substrate...
  2. Chymase-Mediated MMP Activation in Ishemia Reperfusion Injury
    LOUIS DELL ITALIA; Fiscal Year: 2009
    ..Recent studies demonstrated activated MMP-2 within the cardiomyocyte that cleaved myofibrils (4-6) and nuclear matrix protein poly ADP-ribose polymerase (PARP)-a DNA repair enzyme in cell survival during I/..
  3. Role of troponin T isoforms in nemaline myopathy
    Jian Ping Jin; Fiscal Year: 2009
    ..This truncated slow TnT cannot incorporate into myofibrils and is degraded inside the myocyte...
  4. Role of troponin T isoforms in nemaline myopathy
    Jian Ping Jin; Fiscal Year: 2010
    ..This truncated slow TnT cannot incorporate into myofibrils and is degraded inside the myocyte...
  5. Proteolytic Regulation of Troponin T & Cardiac Function
    Jian Ping Jin; Fiscal Year: 2010
    ..of cTnT selectively removes the N-terminal variable region and keeps the remaining conserved structure in the myofibrils with functional consequences...
  6. Proteolytic Regulation of Troponin T &Cardiac Function
    Jian Ping Jin; Fiscal Year: 2011
    ..of cTnT selectively removes the N-terminal variable region and keeps the remaining conserved structure in the myofibrils with functional consequences...
  7. COMPLEX III AUGMENTS OXIDANTS DAMAGE IN ISCHEMIC AGED HEART
    EDWARD LESNEFSKY JR; Fiscal Year: 2004
    ..impairment is selective to the interfibrillar population of cardiac mitochondria 0FM) that reside among the myofibrils, whereas subsarcolemmal mitochondria (SSM), located beneath the plasma membrane, are unaltered...
  8. MUSCLE PROTEIN TURNOVER AND AMINO ACID UPTAKE IN SEPSIS
    Per Olof Hasselgren; Fiscal Year: 2000
    ..Because intact myofibrils are not degraded by the proteasome, it is possible that actin and myosin are dissociated from the myofibrils ..
  9. MUSCLE PROTEIN TURNOVER AND AMINO ACID UPTAKE IN SEPSIS
    Per Olof Hasselgren; Fiscal Year: 2003
    ..Because intact myofibrils are not degraded by the proteasome, it is possible that actin and myosin are dissociated from the myofibrils ..
  10. MUSCLE PROTEIN TURNOVER AND AMINO ACID UPTAKE IN SEPSIS
    Per Olof Hasselgren; Fiscal Year: 2001
    ..Because intact myofibrils are not degraded by the proteasome, it is possible that actin and myosin are dissociated from the myofibrils ..
  11. MUSCLE PROTEIN TURNOVER AND AMINO ACID UPTAKE IN SEPSIS
    Per Olof Hasselgren; Fiscal Year: 2001
    ..Because intact myofibrils are not degraded by the proteasome, it is possible that actin and myosin are dissociated from the myofibrils ..
  12. MUSCLE PROTEIN TURNOVER AND AMINO ACID UPTAKE IN SEPSIS
    Per Olof Hasselgren; Fiscal Year: 2002
    ..Because intact myofibrils are not degraded by the proteasome, it is possible that actin and myosin are dissociated from the myofibrils ..
  13. Role of Troponin T Isoforms in Amish Nemaline Myopathy
    Jian Ping Jin; Fiscal Year: 2007
    ..protein at amino acid 179 We have shown that the truncated slow TnT is degraded and not incorporated into the myofibrils, consistent with the recessive inheritance of the disease The ANM phenotypes include tremors, contractures and ..
  14. Myosin Phosphatase Regulation in Vascular Smooth Muscle
    HOWARD SURKS; Fiscal Year: 2005
    ..Further preliminary studies show that M-RIP colocalizes with myofibrils in VSMCs, and MRIP itself is a Rho-kinase substrate...
  15. Myosin Light Chain 2 Phosphorylation in Cardiac Biology
    NATHAN JIDEAMA; Fiscal Year: 2004
    Cardiac muscle contraction is initiated by morphological changes in the contractile proteins of the cardiac myofibrils. Phosphorylation of myosin light chain 2 (MLC2) and other contractile proteins (troponin T (TnT) and troponin I (TnI)) ..
  16. PHYSIOLOGY OF RESPIRATORY MUSCLE MICRO MECHANICS
    ALADIN BORIEK; Fiscal Year: 2004
    ..and longitudinal mechanical properties of the diaphragm, and the strength of the mechanical linkage between myofibrils and the plasma membrane is determined primarily by these proteins; and (3) the mechanical coupling between ..
  17. PHYSIOLOGY OF RESPIRATORY MUSCLE MIRCO MECHANICS
    ALADIN BORIEK; Fiscal Year: 2002
    ..and longitudinal mechanical properties of the diaphragm, and the strength of the mechanical linkage between myofibrils and the plasma membrane is determined primarily by these proteins; and (3) the mechanical coupling between ..
  18. PHYSIOLOGY OF RESPIRATORY MUSCLE MICRO MECHANICS
    ALADIN BORIEK; Fiscal Year: 2004
    ..and longitudinal mechanical properties of the diaphragm, and the strength of the mechanical linkage between myofibrils and the plasma membrane is determined primarily by these proteins; and (3) the mechanical coupling between ..
  19. PHYSIOLOGY OF RESPIRATORY MUSCLE MIRCO MECHANICS
    ALADIN BORIEK; Fiscal Year: 2001
    ..and longitudinal mechanical properties of the diaphragm, and the strength of the mechanical linkage between myofibrils and the plasma membrane is determined primarily by these proteins; and (3) the mechanical coupling between ..
  20. PHYSIOLOGY OF RESPIRATORY MUSCLE MIRCO MECHANICS
    ALADIN BORIEK; Fiscal Year: 2003
    ..and longitudinal mechanical properties of the diaphragm, and the strength of the mechanical linkage between myofibrils and the plasma membrane is determined primarily by these proteins; and (3) the mechanical coupling between ..
  21. PHYSIOLOGY OF RESPIRATORY MUSCLE MIRCO MECHANICS
    ALADIN BORIEK; Fiscal Year: 2002
    ..and longitudinal mechanical properties of the diaphragm, and the strength of the mechanical linkage between myofibrils and the plasma membrane is determined primarily by these proteins; and (3) the mechanical coupling between ..
  22. PHYSIOLOGY OF RESPIRATORY MUSCLE MIRCO MECHANICS
    ALADIN BORIEK; Fiscal Year: 2001
    ..and longitudinal mechanical properties of the diaphragm, and the strength of the mechanical linkage between myofibrils and the plasma membrane is determined primarily by these proteins; and (3) the mechanical coupling between ..
  23. MOLECULAR PATHOGENESIS OF HUMAN DILATED CARDIOMYOPATHY
    SARKIS MARGOSSIAN; Fiscal Year: 1993
    ..The presence or absence of TnT in IDC tissues will be established by Western blots and radioimmunoassays of myofibrils and troponin...
  24. MOLECULAR/CELLULAR BIOLOGY OF CARDIOMYOCYTE DEVELOPMENT
    DONALD FISCHMAN; Fiscal Year: 1990
    ..2. The assembly of striated myofibrils in the earliest myocytes that can be identified in the heart-forming region of chick embryos (stages 4-10)...
  25. Myosin Phosphatase Regulation in Vascular Smooth Muscle
    HOWARD SURKS; Fiscal Year: 2006
    ..Further preliminary studies show that M-RIP colocalizes with myofibrils in VSMCs, and MRIP itself is a Rho-kinase substrate...
  26. Myosin Phosphatase Regulation in Vascular Smooth Muscle
    HOWARD SURKS; Fiscal Year: 2004
    ..Further preliminary studies show that M-RIP colocalizes with myofibrils in VSMCs, and MRIP itself is a Rho-kinase substrate...
  27. Studies on a Novel RNA that Promotes Heart Development
    LARRY LEMANSKI; Fiscal Year: 2003
    ..development, because homozygous embryos (c/c) form hearts that are deficient in tropomyosin, lack organized myofibrils, and fail to beat...
  28. Intermediate Filaments & Costamere Structure & Function
    Robert J Bloch; Fiscal Year: 2010
    Costameres are structures at the surface of striated muscle that align the sarcolemma regularly with nearby myofibrils and transmit contractile force laterally, through the membrane to the extracellular matrix...
  29. Intermediate Filaments & Costamere Structure & Function
    Robert Bloch; Fiscal Year: 2009
    Costameres are structures at the surface of striated muscle that align the sarcolemma regularly with nearby myofibrils and transmit contractile force laterally, through the membrane to the extracellular matrix...
  30. Studies on a Novel RNA that Promotes Heart Development
    LARRY LEMANSKI; Fiscal Year: 2005
    ..development, because homozygous embryos (c/c) form hearts that are deficient in tropomyosin, lack organized myofibrils, and fail to beat...
  31. Studies on a Novel RNA that Promotes Heart Development
    LARRY LEMANSKI; Fiscal Year: 2004
    ..development, because homozygous embryos (c/c) form hearts that are deficient in tropomyosin, lack organized myofibrils, and fail to beat...
  32. Studies on a Novel RNA that Promotes Heart Development
    LARRY LEMANSKI; Fiscal Year: 2006
    ..development, because homozygous embryos (c/c) form hearts that are deficient in tropomyosin, lack organized myofibrils, and fail to beat...
  33. Studies on a Novel RNA that Promotes Heart Development
    LARRY LEMANSKI; Fiscal Year: 2006
    ..development, because homozygous embryos (c/c) form hearts that are deficient in tropomyosin, lack organized myofibrils, and fail to beat...
  34. The Sarcolemma in FSHD and in the myd Mouse
    Robert Bloch; Fiscal Year: 2003
    ..the sarcolemma of FSHD muscle has frequent interruptions in its membrane skeleton, is separated from the nearest myofibrils by a considerable gap, and is organized irregularly, and most closely resembles the sarcolemma of slow twitch ..
  35. The Sarcolemma in FSHD and in the myd Mouse
    Robert Bloch; Fiscal Year: 2002
    ..the sarcolemma of FSHD muscle has frequent interruptions in its membrane skeleton, is separated from the nearest myofibrils by a considerable gap, and is organized irregularly, and most closely resembles the sarcolemma of slow twitch ..
  36. Sarcolemma in FSHD
    Robert Bloch; Fiscal Year: 2001
    ..the sarcolemma of FSHD muscle has frequent interruptions in its membrane skeleton, is separated from the nearest myofibrils by a considerable gap, and is organized irregularly, and most closely resembles the sarcolemma of slow twitch ..
  37. ALTERED MATRIX METALLOPROTEINASES IN HEART FAILURE
    JEANINE D ARMIENTO; Fiscal Year: 2002
    ..integrity and tensile strength to the myocardium critical to maintaining the alignment of myocytes and myofibrils, and the overall geometry of the left ventricle...
  38. GENE EXPRESSION DURING DROSOPHILA MYOGENESIS
    Allen Shearn; Fiscal Year: 1999
    ..We will use polarized light microscopy and anti-myosin antibody decoration to recognize mutants having defective myofibrils. Since the locations of all contractile protein genes of Drosophila are known, this project will provide an ..
  39. ALTERED MATRIX METALLOPROTEINASES IN HEART FAILURE
    JEANINE D ARMIENTO; Fiscal Year: 1999
    ..integrity and tensile strength to the myocardium critical to maintaining the alignment of myocytes and myofibrils, and the overall geometry of the left ventricle...
  40. ALTERED MATRIX METALLOPROTEINASES IN HEART FAILURE
    JEANINE D ARMIENTO; Fiscal Year: 2000
    ..integrity and tensile strength to the myocardium critical to maintaining the alignment of myocytes and myofibrils, and the overall geometry of the left ventricle...
  41. ALTERED MATRIX METALLOPROTEINASES IN HEART FAILURE
    JEANINE D ARMIENTO; Fiscal Year: 2001
    ..integrity and tensile strength to the myocardium critical to maintaining the alignment of myocytes and myofibrils, and the overall geometry of the left ventricle...
  42. MYOCARDIAL ISCHEMIA
    Robert Jennings; Fiscal Year: 1990
    ..and other cytoskeletal proteins which are involved in the attachment of the sarcolemma to the underlying myofibrils. The effect of endogenous proteases or sarcolemmal and cytoskeletal proteins will be studied...
  43. NOVEL RNA APPROACH THAT PROMOTES HEART DEV
    LARRY LEMANSKI; Fiscal Year: 2000
    ..recessive (c/c) mutant embryos do not beat, as they are deficient in tropomyosin and do not contain organized myofibrils. However, the defect can be corrected by culturing the mutant hearts in the presence of normal anterior endoderm ..
  44. NOVEL RNA APPROACH THAT PROMOTES HEART DEV
    LARRY LEMANSKI; Fiscal Year: 1999
    ..recessive (c/c) mutant embryos do not beat, as they are deficient in tropomyosin and do not contain organized myofibrils. However, the defect can be corrected by culturing the mutant hearts in the presence of normal anterior endoderm ..
  45. STUDIES OF A NOVEL RNA APPROACH THAT PROMOTES HEART DEV
    LARRY LEMANSKI; Fiscal Year: 2001
    ..recessive (c/c) mutant embryos do not beat, as they are deficient in tropomyosin and do not contain organized myofibrils. However, the defect can be corrected by culturing the mutant hearts in the presence of normal anterior endoderm ..
  46. STUDIES OF A NOVEL RNA APPROACH THAT PROMOTES HEART DEV
    LARRY LEMANSKI; Fiscal Year: 2001
    ..recessive (c/c) mutant embryos do not beat, as they are deficient in tropomyosin and do not contain organized myofibrils. However, the defect can be corrected by culturing the mutant hearts in the presence of normal anterior endoderm ..
  47. MYOSIN MOTOR IN ACTION
    Clara Franzini Armstrong; Fiscal Year: 2002
    ..Ultrastructure of myofibrils and thick filaments from the indirect flight muscle (IFM) of transgenic flies will serve as basis for assessing ..
  48. MYOSIN MOTOR IN ACTION
    Clara Franzini Armstrong; Fiscal Year: 2001
    ..Ultrastructure of myofibrils and thick filaments from the indirect flight muscle (IFM) of transgenic flies will serve as basis for assessing ..
  49. MYOSIN MOTOR IN ACTION
    Clara Franzini Armstrong; Fiscal Year: 1999
    ..Ultrastructure of myofibrils and thick filaments from the indirect flight muscle (IFM) of transgenic flies will serve as basis for assessing ..
  50. MYOSIN MOTOR IN ACTION
    Clara Franzini Armstrong; Fiscal Year: 2000
    ..Ultrastructure of myofibrils and thick filaments from the indirect flight muscle (IFM) of transgenic flies will serve as basis for assessing ..
  51. Role of Troponin T Isoforms in Amish Nemaline Myopathy
    Jian Ping Jin; Fiscal Year: 2003
    ..We have shown that the truncated slow TnT is degraded and not incorporated into the myofibrils, consistent with the recessive inheritance of the disease...
  52. Role of Troponin T Isoforms in Amish Nemaline Myopathy
    Jian Ping Jin; Fiscal Year: 2006
    ..We have shown that the truncated slow TnT is degraded and not incorporated into the myofibrils, consistent with the recessive inheritance of the disease...
  53. MUTATION OF FUNCTIONAL DOMAINS IN CARDIAC TROPONIN C
    JOHN PUTKEY; Fiscal Year: 1990
    ..responsible for initial reception of the Ca2+ signal and transmission of this information through a cascade of myofibrils regulatory and contractile proteins...
  54. Costamere Defects in Muscular Dystrophies
    JAMES ERVASTI; Fiscal Year: 2007
    ..assemblies in striated muscle cells that circumferentially align in register with the Z disk of peripheral myofibrils and physically couple force-generating sarcomeres with the sarcolemma...
  55. Costamere Defects in Muscular Dystrophies
    JAMES ERVASTI; Fiscal Year: 2006
    ..assemblies in striated muscle cells that circumferentially align in register with the Z disk of peripheral myofibrils and physically couple force-generating sarcomeres with the sarcolemma...
  56. Costamere Defects in Muscular Dystrophies
    JAMES ERVASTI; Fiscal Year: 2005
    ..assemblies in striated muscle cells that circumferentially align in register with the Z disk of peripheral myofibrils and physically couple force-generating sarcomeres with the sarcolemma...
  57. Costamere Defects in Muscular Dystrophies
    JAMES ERVASTI; Fiscal Year: 2006
    ..assemblies in striated muscle cells that circumferentially align in register with the Z disk of peripheral myofibrils and physically couple force-generating sarcomeres with the sarcolemma...
  58. MECHANISMS OF HEART INDUCTION AND MYOFIBRILLOGENESIS
    LARRY LEMANSKI; Fiscal Year: 1990
    ..specific proteins into cultured heart cells will be employed to examine their incorporation into the myofibrils and/or cytoskeletal elements of the living differentiating cells...
  59. FROM CARDIAC MUSCLE TO VENTRICLE
    William Hunter; Fiscal Year: 1999
    ..The project will test 3 hypotheses: (1) Mechanical coupling between active myofibrils and the cytoskeletal-extracellular matrix causes significant stiffening of systolic myocardium against shear and ..
  60. REGULATION OF MYOTENDINOUS JUNCTION STRUCTURE
    James Tidball; Fiscal Year: 1993
    ..Phosphorylation of MTJ structural proteins(s) results in dissociation of myofibrils from the MTJ membrane...
  61. MUSCLES RELATED TO HEART DISEASE
    YALE GOLDMAN; Fiscal Year: 1993
    ..in live, differentiating cells and the molecular motions and reaction kinetics in fully constituted myofibrils. The objective is to understand how contractile proteins form highly specific, functional macromolecular ..
  62. LISST
    David Sullivan; Fiscal Year: 1999
    ..of the assembly process will be characterized by further development of an in vitro assay which uses isolated myofibrils as a platform on which to analyze the mechanism through which the glycolytic complex is assembled and organized ..
  63. CA2+ REGULATION OF MUSCLE CONTRACTION
    James Potter; Fiscal Year: 1990
    ..other related phenomena in systems of increasing complexity and structural integrity, including thin filaments, myofibrils, skinned fibers and intact muscle fibers...
  64. E-C COUPLING IN NORMAL AND FATIGUED MUSCLES
    Hugo Gonzalez Serratos; Fiscal Year: 1992
    ..We have found that fatigue in frog fibers is associated with a lack of activation of groups of myofibrils and with a localized lack of Ca release in the muscle cross-sectional area...
  65. Costamere Defects in Muscular Dystrophies
    JAMES ERVASTI; Fiscal Year: 2009
    ..assemblies in striated muscle cells that circumferentially align in register with the Z disk of peripheral myofibrils and physically couple force-generating sarcomeres with the sarcolemma...