Summary: The body fluid that circulates in the vascular system (BLOOD VESSELS). Whole blood includes PLASMA and BLOOD CELLS.

Top Publications

  1. ncbi In vitro amplification of PrPSc derived from the brain and blood of sheep infected with scrapie
    Leigh Thorne
    Department of Molecular Pathogenesis and Genetics, Veterinary Laboratories Agency, New Haw, Addlestone, Surrey KT15 3NB, UK
    J Gen Virol 89:3177-84. 2008
  2. pmc An interferon-inducible neutrophil-driven blood transcriptional signature in human tuberculosis
    Matthew P R Berry
    Division of Immunoregulation, MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK
    Nature 466:973-7. 2010
  3. ncbi Quantitative expression of toll-like receptor 1-10 mRNA in cellular subsets of human peripheral blood mononuclear cells and sensitivity to CpG oligodeoxynucleotides
    Veit Hornung
    Department of Internal Medicine, Division of Clinical Pharmacology, University of Munich, Munich, Germany
    J Immunol 168:4531-7. 2002
  4. pmc A large proportion of asymptomatic Plasmodium infections with low and sub-microscopic parasite densities in the low transmission setting of Temotu Province, Solomon Islands: challenges for malaria diagnostics in an elimination setting
    Ivor Harris
    Australian Army Malaria Institute, Weary Dunlop Drive, Gallipoli Barracks Enoggera, QLD 4051, Australia
    Malar J 9:254. 2010
  5. pmc Local macrophage proliferation, rather than recruitment from the blood, is a signature of TH2 inflammation
    Stephen J Jenkins
    Centre for Immunity, Infection and Evolution, and the Institute for Immunology and Infection Research, School of Biological Sciences, University of Edinburgh, Edinburgh EH9 3JT, UK
    Science 332:1284-8. 2011
  6. pmc New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq
    Thomas D Otto
    Parasite Genomics, Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK
    Mol Microbiol 76:12-24. 2010
  7. ncbi The peripheral blood transcriptome dynamically reflects system wide biology: a potential diagnostic tool
    Choong Chin Liew
    ChondroGene, Inc, Toronto, Ontario, Canada
    J Lab Clin Med 147:126-32. 2006
  8. pmc Failure to confirm XMRV/MLVs in the blood of patients with chronic fatigue syndrome: a multi-laboratory study
    Graham Simmons
    Blood Systems Research Institute and University of California, San Francisco, San Francisco, CA 94118, USA
    Science 334:814-7. 2011
  9. pmc Comparative SILAC proteomic analysis of Trypanosoma brucei bloodstream and procyclic lifecycle stages
    Michael D Urbaniak
    Division of Biological Chemistry and Drug Discovery, College of Life Sciences, University of Dundee, Dundee, United Kingdom
    PLoS ONE 7:e36619. 2012
  10. pmc Threat of dengue to blood safety in dengue-endemic countries
    Annelies Wilder-Smith
    Department of Medicine, National University Singapore, Singapore
    Emerg Infect Dis 15:8-11. 2009

Research Grants

Detail Information

Publications242 found, 100 shown here

  1. ncbi In vitro amplification of PrPSc derived from the brain and blood of sheep infected with scrapie
    Leigh Thorne
    Department of Molecular Pathogenesis and Genetics, Veterinary Laboratories Agency, New Haw, Addlestone, Surrey KT15 3NB, UK
    J Gen Virol 89:3177-84. 2008
    ..polyanion, polyadenylic acid (PolyA), facilitating rapid detection of low levels of PrPSc from body fluids such as blood. To this end PrPSc was amplified, in a 3 day PMCA assay, from blood leukocyte preparations from VRQ/VRQ scrapie-..
  2. pmc An interferon-inducible neutrophil-driven blood transcriptional signature in human tuberculosis
    Matthew P R Berry
    Division of Immunoregulation, MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK
    Nature 466:973-7. 2010
    ..Here we identify a whole-blood 393 transcript signature for active TB in intermediate and high-burden settings, correlating with radiological ..
  3. ncbi Quantitative expression of toll-like receptor 1-10 mRNA in cellular subsets of human peripheral blood mononuclear cells and sensitivity to CpG oligodeoxynucleotides
    Veit Hornung
    Department of Internal Medicine, Division of Clinical Pharmacology, University of Munich, Munich, Germany
    J Immunol 168:4531-7. 2002
    ..that PDC and B cells, but not monocytes, NK cells, or T cells, are primary targets of CpG ODN in peripheral blood. The characteristic expression pattern of TLR1-10 in cellular subsets of human PBMC is consistent with the concept ..
  4. pmc A large proportion of asymptomatic Plasmodium infections with low and sub-microscopic parasite densities in the low transmission setting of Temotu Province, Solomon Islands: challenges for malaria diagnostics in an elimination setting
    Ivor Harris
    Australian Army Malaria Institute, Weary Dunlop Drive, Gallipoli Barracks Enoggera, QLD 4051, Australia
    Malar J 9:254. 2010
  5. pmc Local macrophage proliferation, rather than recruitment from the blood, is a signature of TH2 inflammation
    Stephen J Jenkins
    Centre for Immunity, Infection and Evolution, and the Institute for Immunology and Infection Research, School of Biological Sciences, University of Edinburgh, Edinburgh EH9 3JT, UK
    Science 332:1284-8. 2011
    ..of inflammation is the accumulation of innate immune cells in the tissue that are thought to be recruited from the blood. We reveal that a distinct process exists in which tissue macrophages undergo rapid in situ proliferation in order ..
  6. pmc New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq
    Thomas D Otto
    Parasite Genomics, Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK
    Mol Microbiol 76:12-24. 2010
    ..These data reveal new features of the P. falciparum transcriptional landscape and significantly advance our understanding of the parasite's red blood cell-stage transcriptome.
  7. ncbi The peripheral blood transcriptome dynamically reflects system wide biology: a potential diagnostic tool
    Choong Chin Liew
    ChondroGene, Inc, Toronto, Ontario, Canada
    J Lab Clin Med 147:126-32. 2006
    In our genome-wide survey of gene expression in human peripheral blood cells using both an expressed sequence tag (EST) and a microarray hybridization approach, we identified the expression of a large proportion (approximately 80%) of ..
  8. pmc Failure to confirm XMRV/MLVs in the blood of patients with chronic fatigue syndrome: a multi-laboratory study
    Graham Simmons
    Blood Systems Research Institute and University of California, San Francisco, San Francisco, CA 94118, USA
    Science 334:814-7. 2011
    ..To explore this issue in greater depth, we compiled coded replicate samples of blood from 15 subjects previously reported to be XMRV/MLV-positive (14 with CFS) and from 15 healthy donors previously ..
  9. pmc Comparative SILAC proteomic analysis of Trypanosoma brucei bloodstream and procyclic lifecycle stages
    Michael D Urbaniak
    Division of Biological Chemistry and Drug Discovery, College of Life Sciences, University of Dundee, Dundee, United Kingdom
    PLoS ONE 7:e36619. 2012
    ..Our proteomic data is broadly in agreement with transcriptomic studies, but with significantly larger fold changes observed at the protein level than at the mRNA level...
  10. pmc Threat of dengue to blood safety in dengue-endemic countries
    Annelies Wilder-Smith
    Department of Medicine, National University Singapore, Singapore
    Emerg Infect Dis 15:8-11. 2009
    ..Healthcare-related transmission, including transmission by blood products, has been documented, although the frequency of these occurrences is unknown...
  11. pmc Metabolomics analysis reveals large effects of gut microflora on mammalian blood metabolites
    William R Wikoff
    Department of Molecular Biology and Center for Mass Spectrometry, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 106:3698-703. 2009
    ..MS-based metabolomics study that demonstrates a surprisingly large effect of the gut "microbiome" on mammalian blood metabolites...
  12. pmc Multiplexed real-time PCR assay for discrimination of Plasmodium species with improved sensitivity for mixed infections
    Sandra E Shokoples
    Provincial Laboratory for Public Health, Edmonton, Alberta, Canada
    J Clin Microbiol 47:975-80. 2009
    ..This assay was further validated with 91 blood samples and demonstrated a specificity and sensitivity for single infections of 100% compared with nested PCR as ..
  13. pmc Diagnosis of bacteremia in whole-blood samples by use of a commercial universal 16S rRNA gene-based PCR and sequence analysis
    Nele Wellinghausen
    Institut fur Medizinische Mikrobiologie und Hygiene, Universitatsklinikum Ulm, Albert Einstein Allee 23, D 89081 Ulm, Germany
    J Clin Microbiol 47:2759-65. 2009
    In a prospective, multicenter study of 342 blood samples from 187 patients with systemic inflammatory response syndrome, sepsis, or neutropenic fever, a new commercial PCR test (SepsiTest; Molzym) was evaluated for rapid diagnosis of ..
  14. pmc Dried blood spots as a source of anti-malarial antibodies for epidemiological studies
    Patrick H Corran
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London WC1E 7HT, UK
    Malar J 7:195. 2008
    b>Blood spots collected onto filter paper are an established and convenient source of antibodies for serological diagnosis and epidemiological surveys...
  15. ncbi Blood-feeding behaviour of the malarial mosquito Anopheles arabiensis: implications for vector control
    I Tirados
    FARM Africa, London, U K
    Med Vet Entomol 20:425-37. 2006
    ..but also takes a high proportion of meals from non-human hosts, with 59-91% of resting mosquitoes containing blood from cattle...
  16. ncbi Blood-feeding behavior of Aedes albopictus, a vector of Chikungunya on La Réunion
    Helene Delatte
    Research Unit 016, Institut de recherche pour le developpement IRD, Saint Pierre, France
    Vector Borne Zoonotic Dis 10:249-58. 2010
    ..Multiple blood feeding was also reported in every combination (animal/human) offered to Ae...
  17. ncbi Bright-blood T2-weighted MRI has higher diagnostic accuracy than dark-blood short tau inversion recovery MRI for detection of acute myocardial infarction and for assessment of the ischemic area at risk and myocardial salvage
    Alexander R Payne
    West of Scotland Heart and Lung Centre, Golden Jubilee National Hospital, Glasgow, Scotland, United Kingdom
    Circ Cardiovasc Imaging 4:210-9. 2011
    ..We compared the diagnostic accuracy of a new bright-blood T2-weighted with a standard black blood T2-weighted MRI in patients with acute MI.
  18. ncbi Blood-feeding and immunogenic Aedes aegypti saliva proteins
    Ladawan Wasinpiyamongkol
    Department of Tropical Hygiene, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
    Proteomics 10:1906-16. 2010
    ..What occurs in these organs in response to a blood meal is poorly understood, but identifying the physiological differences between sugar-fed and blood-fed (BF) ..
  19. pmc School-based surveys of malaria in Oromia Regional State, Ethiopia: a rapid survey method for malaria in low transmission settings
    Ruth A Ashton
    Malaria Consortium Ethiopia, PO Box 110224, Ethio China Road, Addis Ababa, Ethiopia
    Malar J 10:25. 2011
  20. pmc Methylation at global LINE-1 repeats in human blood are affected by gender but not by age or natural hormone cycles
    Osman El-Maarri
    Institute of Experimental Hematology and Transfusion Medicine, University of Bonn, Bonn, Germany
    PLoS ONE 6:e16252. 2011
    ..To this end, we studied LINE-1 methylation in vivo in blood-derived DNA from individuals aged 18 to 64 years and from young healthy females at various hormone levels during ..
  21. pmc A conduit system distributes chemokines and small blood-borne molecules through the splenic white pulp
    Martijn A Nolte
    Department of Molecular Cell Biology, VUMC, Amsterdam, Netherlands
    J Exp Med 198:505-12. 2003
    ..Here we show that movement of molecules from the blood into these confined areas is also limited...
  22. pmc Identification of a third human polyomavirus
    Tobias Allander
    Laboratory for Clinical Microbiology, Department of Clinical Microbiology, Tumor and Cell Biology, L2 02, Karolinska University Hospital, SE 171 76 Stockholm, Sweden
    J Virol 81:4130-6. 2007
    ..5%) of 192 fecal samples but was not detected in sets of urine and blood samples...
  23. pmc Accurate real-time PCR strategy for monitoring bloodstream parasitic loads in chagas disease patients
    Tomas Duffy
    Laboratorio de Biologia Molecular de la Enfermedad de Chagas, Instituto de Investigaciones en Ingeniería Genética y Biología Molecular INGEBI CONICET, Buenos Aires, Argentina
    PLoS Negl Trop Dis 3:e419. 2009
    This report describes a real-time PCR (Q-PCR) strategy to quantify Trypanosoma cruzi (T. cruzi) DNA in peripheral blood samples from Chagas disease patients targeted to conserved motifs within the repetitive satellite sequence.
  24. pmc Reconsideration of Anopheles rivulorum as a vector of Plasmodium falciparum in western Kenya: some evidence from biting time, blood preference, sporozoite positive rate, and pyrethroid resistance
    Hitoshi Kawada
    Department of Vector Ecology and Environment, Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan
    Parasit Vectors 5:230. 2012
    ..funestus group. The role of An. rivulorum as a malaria vector has not been fully studied, although it has been found to be a minor or opportunistic transmitter of Plasmodium falciparum...
  25. pmc Human Plasmodium knowlesi infections in young children in central Vietnam
    Peter Van den Eede
    Department of Parasitology, Institute of Tropical Medicine, Antwerp, Belgium
    Malar J 8:249. 2009
    Considering increasing reports on human infections by Plasmodium knowlesi in Southeast Asian countries, blood samples collected during two large cross-sectional malariometric surveys carried out in a forested area of central Vietnam in ..
  26. pmc Skewed pattern of Toll-like receptor 4-mediated cytokine production in human neonatal blood: low LPS-induced IL-12p70 and high IL-10 persist throughout the first month of life
    M E Belderbos
    Wilhelmina Children s Hospital, University Medical Centre Utrecht, Utrecht, The Netherlands
    Clin Immunol 133:228-37. 2009
    ..Cellularity and Toll-like receptor (TLR) agonist-induced cytokine production were compared between cord blood obtained from healthy neonates born after uncomplicated gestation and delivery (n=18), neonatal venous blood ..
  27. pmc 3D microfilter device for viable circulating tumor cell (CTC) enrichment from blood
    Siyang Zheng
    Department of Bioengineering, Pennsylvania State University, University Park, PA 16802, USA
    Biomed Microdevices 13:203-13. 2011
    ..We report a novel three dimensional microfilter device that can enrich viable circulating tumor cells from blood. This device consists of two layers of parylene membrane with pores and gap precisely defined with ..
  28. pmc Effect of factor H-binding protein sequence variation on factor H binding and survival of Neisseria meningitidis in human blood
    Kathleen Y Dunphy
    Center for Immunobiology and Vaccine Development, Children s Hospital Oakland Research Institute, 5700 Martin Luther King Jr Way, Oakland, CA 94609, USA
    Infect Immun 79:353-9. 2011
    ..When incubated in plasma or blood of nonimmune donors, all three mutants showed similar increases in CFU/ml...
  29. ncbi Minority HIV mutation detection in dried blood spots indicates high specimen integrity and reveals hidden archived drug resistance
    Xierong Wei
    Laboratory Branch, Division of HIV AIDS Prevention, National Centers for HIV AIDS, Viral Hepatitis, STD, and TB Prevention, Centers for Disease Control and Prevention, 1600 Clifton Rd, NE, MS G45 Atlanta, GA 30333, USA
    J Clin Virol 50:148-52. 2011
    Dried blood spots (DBS) could serve as an attractive, cost-effective alternative to plasma for HIV drug resistance testing.
  30. pmc Factor H-binding protein is important for meningococcal survival in human whole blood and serum and in the presence of the antimicrobial peptide LL-37
    K L Seib
    Molecular Genetics Unit, Novartis Vaccines, Via Fiorentina, 1, 53100 Siena, Italy
    Infect Immun 77:292-9. 2009
    ..All fHBP null mutant strains analyzed were sensitive to killing in ex vivo human whole blood and serum models of meningococcal bacteremia with respect to the isogenic wild-type strains...
  31. ncbi Species identification and molecular characterization of Acinetobacter spp. blood culture isolates from Norway
    Nabil Karah
    Reference Centre for Detection of Antimicrobial Resistance, Department of Microbiology and Infection Control, University Hospital of North Norway, and Department of Medical Biology, Faculty of Health Sciences, University of Tromsø, Tromsø, Norway
    J Antimicrob Chemother 66:738-44. 2011
    ..investigated the species distribution, antibiotic susceptibility patterns and genotypic resistance characteristics of 113 consecutive blood culture isolates of Acinetobacter species collected between 2005 and 2007 throughout Norway.
  32. ncbi An evaluation of urine-CCA strip test and fingerprick blood SEA-ELISA for detection of urinary schistosomiasis in schoolchildren in Zanzibar
    J Russell Stothard
    Wolfson Wellcome Biomedical Laboratories, Department of Zoology, Natural History Museum, London, UK
    Acta Trop 111:64-70. 2009
    ..By contrast, the performance of the SEA-ELISA using sera from fingerprick blood was good; a sensitivity of 89% (95% CI=76-96%), a specificity of 70% (95% CI=60-79%), a positive predictive value ..
  33. pmc Optimal deconvolution of transcriptional profiling data using quadratic programming with application to complex clinical blood samples
    Ting Gong
    Biomarker Development, Novartis Institutes for BioMedical Research, Cambridge, Massachusetts, United States of America
    PLoS ONE 6:e27156. 2011
    ..These data suggest that our method can solve one of the open questions regarding the analysis of complex transcriptional data: namely, how to identify the optimal mixing fractions in a given experiment...
  34. pmc Drinking a hot blood meal elicits a protective heat shock response in mosquitoes
    Joshua B Benoit
    Department of Entomology and Evolution, Ecology and Organismal Biology, The Ohio State University, Columbus, OH 43210, USA
    Proc Natl Acad Sci U S A 108:8026-9. 2011
    The mosquito's body temperature increases dramatically when it takes a blood meal from a warm-blooded, vertebrate host...
  35. ncbi Calculation of liver-to-blood inocula, parasite growth rates, and preerythrocytic vaccine efficacy, from serial quantitative polymerase chain reaction studies of volunteers challenged with malaria sporozoites
    Philip Bejon
    Centre for Clinical Vaccinology and Tropical Medicine, University of Oxford, Oxford, United Kingdom
    J Infect Dis 191:619-26. 2005
    ..This was done to measure vaccine efficacy and to distinguish the effects on blood-stage multiplication from those on liver-stage parasites...
  36. ncbi Preliminary clinical study using a multiplex real-time PCR test for the detection of bacterial and fungal DNA directly in blood
    F Wallet
    Pole de Microbiologie, Centre Hospitalier Universitaire de Lille, Faculte de Medicine de Lille, France
    Clin Microbiol Infect 16:774-9. 2010
    ..species level and four groups of pathogens at the genus level has been evaluated and compared with conventional blood cultures (BCs)...
  37. ncbi Granulocytes govern the transcriptional response, morphology and proliferation of Candida albicans in human blood
    Chantal Fradin
    Robert Koch Institut, Nordufer 20, 13353 Berlin, Germany
    Mol Microbiol 56:397-415. 2005
    Survival in blood and escape from blood vessels into tissues are essential steps for the yeast Candida albicans to cause systemic infections...
  38. ncbi Role of arthropod saliva in blood feeding: sialome and post-sialome perspectives
    Jose M C Ribeiro
    Medical Entomology Section, Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 4 Center Drive, Bethesda, Maryland, 20892 0425, USA
    Annu Rev Entomol 48:73-88. 2003
    This review addresses the problems insects and ticks face to feed on blood and the solutions these invertebrates engender to overcome these obstacles, including a sophisticated salivary cocktail of potent pharmacologic compounds...
  39. pmc Reliable enumeration of malaria parasites in thick blood films using digital image analysis
    John A Frean
    National Institute for Communicable Diseases, P Bag X4, Sandringham 2131, Johannesburg, South Africa
    Malar J 8:218. 2009
    ..Microscopy of Giemsa-stained thick blood films is the conventional method for parasite enumeration...
  40. ncbi Variable contexts and levels of hypermutation in HIV-1 proviral genomes recovered from primary peripheral blood mononuclear cells
    Gustavo H Kijak
    Henry M Jackson Foundation for the Advancement of Military Medicine US Military HIV Research Program, 1600 East Gude Drive, Rockville, Maryland 20850, USA
    Virology 376:101-11. 2008
    ..These data suggest considerable complexity in the interplay of host restriction and viral defense during HIV-1 infection...
  41. ncbi Fungal pathogen reduces potential for malaria transmission
    Simon Blanford
    Institutes of Evolution, Immunology, and Infection Research, School of Biological Sciences, Ashworth Laboratories, University of Edinburgh, Edinburgh EH9 3JT Scotland, UK
    Science 308:1638-41. 2005
    ..malaria model, we found that exposure to surfaces treated with fungal entomopathogens following an infectious blood meal reduced the number of mosquitoes able to transmit malaria by a factor of about 80...
  42. ncbi Human leptin signaling in human peripheral blood mononuclear cells: activation of the JAK-STAT pathway
    V Sanchez-Margalet
    Department of Medical Biochemistry and Molecular Biology, School of Medicine, Investigation Unit, University of Seville, 41009 Seville, Spain
    Cell Immunol 211:30-6. 2001
    ..In this paper, we have studied the signal transduction of the leptin receptor in peripheral blood mononuclear cells. We found that leptin stimulation activates the JAK-STAT signaling pathway...
  43. pmc PCR-based pooling of dried blood spots for detection of malaria parasites: optimization and application to a cohort of Ugandan children
    Michelle S Hsiang
    Department of Global Health Sciences, Division of Pediatric Infectious Diseases, University of California, San Francisco, San Francisco, CA 94105, USA
    J Clin Microbiol 48:3539-43. 2010
    ..This PCR-based pooling strategy was then compared with microscopy using 891 dried blood spots from a cohort of 77 Ugandan children followed for 2 years in an urban setting of low endemicity...
  44. pmc Description of outcomes of experimental infection with feline haemoplasmas: copy numbers, haematology, Coombs' testing and blood glucose concentrations
    Severine Tasker
    School of Clinical Veterinary Science, University of Bristol, Langford, Bristol BS40 5DU, United Kingdom
    Vet Microbiol 139:323-32. 2009
    The aim of this study was to compare blood copy, haematological and glucose values between cats experimentally infected with either Mycoplasma haemofelis (Group HF: 10 cats), 'Candidatus M...
  45. ncbi Early diagnosis of human immunodeficiency virus in infants using polymerase chain reaction on dried blood spots in Botswana's national program for prevention of mother-to-child transmission
    Tracy Creek
    Global AIDS Program, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
    Pediatr Infect Dis J 27:22-6. 2008
    ..Dried blood spot (DBS) polymerase chain reaction (PCR) provides a feasible method to assess PMTCT programs and identify HIV-..
  46. ncbi An antimicrobial stewardship program's impact with rapid polymerase chain reaction methicillin-resistant Staphylococcus aureus/S. aureus blood culture test in patients with S. aureus bacteremia
    Karri A Bauer
    Department of Pharmacy, The Ohio State University Medical Center, Columbus, Ohio 43210, USA
    Clin Infect Dis 51:1074-80. 2010
    ..aureus/S. aureus blood culture test (rPCR). This single‐center study compared inpatients with S...
  47. ncbi Comparison of three real-time PCR methods with blood smears and rapid diagnostic test in Plasmodium sp. infection
    N Bourgeois
    Laboratoire de Parasitologie Mycologie, Centre Hospitalier Universitaire de Nimes, Universite Montpellier I, Nimes, France
    Clin Microbiol Infect 16:1305-11. 2010
    ..The methods used for routine diagnostic malaria testing consisted of microscopic examination of Giemsa-stained blood smears and rapid diagnostic tests...
  48. ncbi Tetracyclines and chemically modified tetracycline-3 (CMT-3) modulate cytokine secretion by lipopolysaccharide-stimulated whole blood
    Julia Cazalis
    Groupe de Recherche en Ecologie Buccale, Faculte de Medecine Dentaire, Universite Laval, Quebec City, Quebec, G1K 7P4, Canada
    Inflammation 32:130-7. 2009
    ..on the production of pro-inflammatory mediators and matrix metalloproteinases (MMPs) in an ex vivo human whole blood (WB) model stimulated with Porphyromonas gingivalis lipopolysaccharide (LPS)...
  49. ncbi Detection of volatile metabolites produced by bacterial growth in blood culture media by selected ion flow tube mass spectrometry (SIFT-MS)
    Randall A Allardyce
    Department of Surgery, Christchurch School of Medicine and Health Sciences, Christchurch, New Zealand
    J Microbiol Methods 65:361-5. 2006
    ..selected ion flow tube mass spectrometry (SIFT-MS) measured metabolic gases in the headspaces of BacT/ALERT blood culture bottles...
  50. pmc Detection of four Plasmodium species by genus- and species-specific loop-mediated isothermal amplification for clinical diagnosis
    Eun Taek Han
    Cell Free Science and Technology Research Center, Ehime University, Matsuyama, Ehime 790 8577, Japan
    J Clin Microbiol 45:2521-8. 2007
    ..falciparum, and P. vivax. LAMP detected malaria parasites in 67 of 68 microscopically positive blood samples (sensitivity, 98.5%) and 3 of 53 microscopically negative samples (specificity, 94...
  51. ncbi The role of a real-time PCR technology for rapid detection and identification of bacterial and fungal pathogens in whole-blood samples
    Hideaki Obara
    Department of Surgery, Keio University School of Medicine, 35 Shinanomachi, Shinjuku ku, Tokyo 160 8582, Japan
    J Infect Chemother 17:327-33. 2011, we evaluated a multiplex polymerase chain reaction (PCR-M) test that detects bacteria and fungi in whole-blood specimens, comparing its features to those of a blood culture (BC)...
  52. pmc Rapid detection of Pseudomonas aeruginosa from positive blood cultures by quantitative PCR
    Vincent Cattoir
    Laboratoire de bactériologie virologie hygiène, Hopital Henri Mondor, Assistance Publique Hopitaux de Paris, Creteil, France
    Ann Clin Microbiol Antimicrob 9:21. 2010
    ..The present study was aimed to develop a novel quantitative PCR (qPCR) assay, using ecfX as the specific target gene, for the rapid and accurate identification of P. aeruginosa from positive blood cultures (BCs).
  53. pmc Predictors of positive blood culture and deaths among neonates with suspected neonatal sepsis in a tertiary hospital, Mwanza-Tanzania
    Neema Kayange
    Department of Pediatric and Child Health Bugando Medical Centre, Mwanza, Tanzania
    BMC Pediatr 10:39. 2010 Bugando Medical Centre (BMC), Tanzania to determine the prevalence of neonatal sepsis, predictors of positive blood culture, deaths and antimicrobial susceptibility, thus providing essential information to formulate a policy for ..
  54. ncbi Supervised blood-based self-sample collection and rapid test performance: a valuable alternative to the use of saliva by HIV testing programmes with no medical or nursing staff
    María J Belza
    Escuela Nacional de Sanidad Instituto de Salud Carlos III C Sinesio Delgado, 8 28029 Madrid, Spain
    Sex Transm Infect 88:218-21. 2012
    ..Many countries require blood-based testing programmes to have doctors/nurses...
  55. pmc Human Plasmodium knowlesi infection in Ranong province, southwestern border of Thailand
    Natthawan Sermwittayawong
    Department of Microbiology, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, Thailand
    Malar J 11:36. 2012
    ..Plasmodium knowlesi, a simian malaria parasite, has been reported in humans in many Southeast Asian countries. In Thailand, most of the limited numbers of cases reported so far were from areas near neighbouring countries, including Myanmar...
  56. ncbi Current and future applications of dried blood spots in viral disease management
    Ingrid J M Snijdewind
    Department of Internal Medicine, Erasmus MC, P O Box 2040, 3000 CA Rotterdam, The Netherlands
    Antiviral Res 93:309-21. 2012
    Almost five decades after their first application in diagnostics, dried blood spot (DBS) cards remain to be of key interest in many research areas and clinical applications...
  57. ncbi Cytomegalovirus quantification: where to next in optimising patient management?
    Claire Atkinson
    Centre for Virology, Department of Infection, UCL London, United Kingdom
    J Clin Virol 51:223-8. 2011
    Over the years quantification of cytomegalovirus (HCMV) load in blood has become a mainstay of clinical management helping direct deployment of antiviral therapy, assess response to therapy and highlight cases of drug resistance.
  58. pmc Prospective study on severe malaria among in-patients at Bombo regional hospital, Tanga, north-eastern Tanzania
    Hamisi A Msangeni
    National Institute for Medical Research, Tanga Medical Research Centre, P O Box 5004, Tanga, Tanzania
    BMC Infect Dis 11:256. 2011
    ..Severe anaemia and cerebral malaria are the two main causes of death due to malaria in Tanga, Tanzania...
  59. pmc Molecular characterisation of transport mechanisms at the developing mouse blood-CSF interface: a transcriptome approach
    Shane A Liddelow
    Department of Pharmacology, The University of Melbourne, Melbourne, Victoria, Australia
    PLoS ONE 7:e33554. 2012
    Exchange mechanisms across the blood-cerebrospinal fluid (CSF) barrier in the choroid plexuses within the cerebral ventricles control access of molecules to the central nervous system, especially in early development when the brain is ..
  60. pmc Efficacy of artemether-lumefantrine in treatment of malaria among under-fives and prevalence of drug resistance markers in Igombe-Mwanza, north-western Tanzania
    Erasmus Kamugisha
    Weill Bugando University College of Health Sciences, Mwanza, Tanzania
    Malar J 11:58. 2012
  61. pmc Acceleration of the direct identification of Staphylococcus aureus versus coagulase-negative staphylococci from blood culture material: a comparison of six bacterial DNA extraction methods
    A J M Loonen
    PAMM Laboratory for Medical Microbiology, Eindhoven Veldhoven, The Netherlands
    Eur J Clin Microbiol Infect Dis 30:337-42. 2011
    ..staphylococci (CNS), this study aimed to compare six different DNA extraction methods from two commonly used blood culture materials, i.e. BACTEC and BacT/ALERT...
  62. ncbi The Kennedy phospholipid biosynthesis pathways are refractory to genetic disruption in Plasmodium berghei and therefore appear essential in blood stages
    Sandrine Dechamps
    Dynamique des Interactions Membranaires Normales et Pathologiques, UMR 5235, CNRS Universite Montpellier 2, Place Eugene Bataillon, cc107, Montpellier 34095, Cedex 05, France
    Mol Biochem Parasitol 173:69-80. 2010
    ..berghei blood stage parasites...
  63. ncbi Insights into blood feeding by schistosomes from a proteomic analysis of worm vomitus
    Stephanie L Hall
    Centre for Immunology and Infection, Department of Biology, University of York, York YO10 5DD, UK
    Mol Biochem Parasitol 179:18-29. 2011
  64. pmc Integration of GWAS SNPs and tissue specific expression profiling reveal discrete eQTLs for human traits in blood and brain
    Dena G Hernandez
    Laboratory of Neurogenetics, National Institute on Aging, National Institutes of Health, Bethesda, MD 20892 3707, USA
    Neurobiol Dis 47:20-8. 2012
    ..present study we used two appropriately powered sample series to examine the genetic control of gene expression in blood and brain...
  65. pmc microRNA miR-275 is indispensable for blood digestion and egg development in the mosquito Aedes aegypti
    Bart Bryant
    Department of Entomology, Institute for Integrative Genome Biology, University of California, Riverside, CA 92521, USA
    Proc Natl Acad Sci U S A 107:22391-8. 2010
    ..Mosquitoes, such as A. aegypti, serve as vectors for disease pathogens because they require vertebrate blood for their egg production...
  66. pmc Absence of evidence of xenotropic murine leukemia virus-related virus infection in persons with chronic fatigue syndrome and healthy controls in the United States
    William M Switzer
    Laboratory Branch, Division of HIV AIDS Prevention, National Center for HIV AIDS, Viral Hepatitis, STD, and TB Prevention, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
    Retrovirology 7:57. 2010
    ..7% of healthy persons from the United States. To investigate the association of XMRV with CFS we tested blood specimens from 51 persons with CFS and 56 healthy persons from the US for evidence of XMRV infection by using ..
  67. pmc Real-time identification of bacteria and Candida species in positive blood culture broths by matrix-assisted laser desorption ionization-time of flight mass spectrometry
    Agnes Ferroni
    Laboratoire de Microbiologie, Assistance Publique Hopitaux de Paris, Hopital Necker Enfants Malades, and Université René Descartes Paris 5, Faculte de Medecine, 149 rue de Sevres, 75015 Paris, France
    J Clin Microbiol 48:1542-8. 2010
    ..In this study, we have adapted and tested this technology for use with blood culture broths, thus allowing identification in less than 30 min once the blood culture is detected as positive...
  68. pmc Giemsa-stained thick blood films as a source of DNA for Plasmodium species-specific real-time PCR
    Lieselotte Cnops
    Department of Clinical Sciences, Institute of Tropical Medicine, Antwerp, Belgium
    Malar J 9:370. 2010
    This study describes the use of thick blood films (TBF) as specimens for DNA amplification with the Plasmodium species-specific real-time PCR that was recently validated on whole blood samples.
  69. pmc Rapid identification of bacterial pathogens in positive blood culture bottles by use of a broad-based PCR assay coupled with high-resolution melt analysis
    Helen Won
    Johns Hopkins University Department of Emergency Medicine, 5801 Smith Avenue, Suite 3220, Davis Building, Baltimore, MD 21209, USA
    J Clin Microbiol 48:3410-3. 2010
    ..With a reference library of 60 clinically relevant bacterial species, 52 positive blood culture samples were tested...
  70. ncbi The multiplicity of Plasmodium falciparum infections is associated with acquired immunity to asexual blood stage antigens
    Pembe Issamou Mayengue
    Unite de Recherches Medicales, Hopital Albert Schweitzer, Lambarene, Gabon
    Microbes Infect 11:108-14. 2009
    ..03) antibody responses to specific asexual stage antigens. Additionally, the whole blood interferon-gamma production capacity was significantly higher (p < 0...
  71. ncbi Detection of enterovirus RNA in peripheral blood mononuclear cells of type 1 diabetic patients beyond the stage of acute infection
    Barbara M Schulte
    Department of Medical Microbiology, Radboud University Nijmegen Medical Centre and Nijmegen Centre for Molecular Life Sciences, Nijmegen, The Netherlands
    Viral Immunol 23:99-104. 2010
    Previous studies have shown that enteroviral RNA can be detected in blood at the onset of type 1 diabetes (T1D). The infection may play a role in triggering T1D and genetic host factors may contribute to this process...
  72. ncbi Scl is required for dorsal aorta as well as blood formation in zebrafish embryos
    Lucy J Patterson
    Weatherall Institute of Molecular Medicine, Oxford University, John Radcliffe Hospital, Headington, Oxford, OX3 9DS, United Kingdom
    Blood 105:3502-11. 2005
    b>Blood and endothelial cells arise in close association in developing embryos, possibly from a shared precursor, the hemangioblast, or as hemogenic endothelium...
  73. ncbi Detection of BK, JC, WU, or KI polyomaviruses in faecal, urine, blood, cerebrospinal fluid and respiratory samples
    Seweryn Bialasiewicz
    Queensland Pediatric Infectious Diseases Laboratory, Sir Albert Sakzewski Virus Research Centre, QLD, Australia
    J Clin Virol 45:249-54. 2009
    ..The related human polyomaviruses JCV and BKV in contrast, have been detected in a wide range of sample types, leading to increased knowledge about their biology and pathogenesis...
  74. pmc High genetic polymorphism of relapsing P. vivax isolates in northwest Colombia
    Eliana Restrepo
    Grupo Salud y Comunidad, Facultad de Medicina, Universidad de Antioquia, Medellin, Colombia
    Acta Trop 119:23-9. 2011
    ..We conclude that analysis of the three microsatellites is a valuable tool to establish the genetic characteristics of P. vivax populations in Colombia...
  75. pmc Contribution of midgut bacteria to blood digestion and egg production in aedes aegypti (diptera: culicidae) (L.)
    Analiz de O Gaio
    Laboratorio de Biotecnologia, Universidade Estadual do Norte Fluminense UENF, 28013 602, Campos dos Goytacazes, RJ, Brazil
    Parasit Vectors 4:105. 2011
    ..In this study, oral administration of antibiotics was employed in order to examine the contribution of gut bacteria to blood digestion and fecundity in Aedes aegypti.
  76. pmc Fluorescence in situ hybridization with peptide nucleic acid probes for rapid identification of Candida albicans directly from blood culture bottles
    Susan Rigby
    Applied Biosystems, Bedford, Massachusetts 01730, USA
    J Clin Microbiol 40:2182-6. 2002
    ..method that uses peptide nucleic acid (PNA) probes for identification of Candida albicans directly from positive-blood-culture bottles in which yeast was observed by Gram staining (herein referred to as yeast-positive blood culture ..
  77. ncbi Toll-like receptor (TLR)2 and TLR4 in human peripheral blood granulocytes: a critical role for monocytes in leukocyte lipopolysaccharide responses
    Ian Sabroe
    Section of Functional Genomics, Division of Genomic Medicine, University of Sheffield, Sheffield, United Kingdom
    J Immunol 168:4701-10. 2002
    ..We conclude that LPS has little role in the regulation of peripheral blood eosinophil and basophil function, and that, even in neutrophils, monocytes orchestrate many previously observed ..
  78. pmc An insulin-like peptide regulates egg maturation and metabolism in the mosquito Aedes aegypti
    Mark R Brown
    Departments of Entomology and Cellular Biology, University of Georgia, Athens, GA 30602, USA
    Proc Natl Acad Sci U S A 105:5716-21. 2008
    Ingestion of vertebrate blood is essential for egg maturation and transmission of disease-causing parasites by female mosquitoes...
  79. pmc Upregulation of PD-1 expression on circulating and intrahepatic hepatitis C virus-specific CD8+ T cells associated with reversible immune dysfunction
    Lucy Golden-Mason
    Division of Gastroenterology Hepatology, Hepatitis C Center, University of Colorado Health Sciences Center and National Jewish Hospital, Denver, CO 80262, USA
    J Virol 81:9249-58. 2007 significantly upregulated on total and HCV-specific CD8(+) cytotoxic T lymphocytes (CTLs) in the peripheral blood and livers of patients with chronic infection compared to subjects with spontaneous HCV resolution, patients with ..
  80. ncbi Differential effects of Lactobacillus acidophilus and Lactobacillus plantarum strains on cytokine induction in human peripheral blood mononuclear cells
    Yvonne M Vissers
    Cell Biology and Immunology Group, Wageningen University and Research Centre, Wageningen, The Netherlands
    FEMS Immunol Med Microbiol 59:60-70. 2010
    ..effect of Lactobacillus species on the cytokine profiles and proliferative response of human peripheral blood mononuclear cells (hPBMC), and in particular, on the comparison between the species Lactobacillus acidophilus and ..
  81. pmc Ozonation of human blood induces a remarkable upregulation of heme oxygenase-1 and heat stress protein-70
    Velio Bocci
    Department of Physiology, University of Siena, 53100 Siena, Italy
    Mediators Inflamm 2007:26785. 2007
    ..Unpublished observations have shown that HO-1 is induced after the infusion of ozonated blood into the respective donors, and many other experimental observations have demonstrated the efficacy of oxidizing ..
  82. ncbi G-CSF control of neutrophils dynamics in the blood
    E Shochat
    Weizmann Institute of Science, Rehovot, Israel
    Bull Math Biol 69:2299-338. 2007
    White blood cell neutrophil is a key component in the fast initial immune response against bacterial and fungal infections...
  83. ncbi Relationship between HIV-RNA load in blood and semen in antiretroviral-naïve and experienced men and effect of asymptomatic sexually transmissible infections
    Derek J Chan
    Albion Street Centre, Prince of Wales Hospital, Sydney, New South Wales, Australia
    Curr HIV Res 6:138-42. 2008
    We assessed the correlation between HIV-RNA viral load in blood (BPVL) and seminal plasma (SPVL) in a cross-sectional cohort of 119 asymptomatic, antiretroviral-naive and experienced HIV-1 subjects (BPVL < 50 copies/mL for minimum 12 ..
  84. pmc Simultaneous detection of Staphylococcus aureus and coagulase-negative staphylococci in positive blood cultures by real-time PCR with two fluorescence resonance energy transfer probe sets
    H Sakai
    Department of Anatomic Pathology, Section of Surgical Pathology, The Cleveland Clinic Foundation, 9500 Euclid Ave, Cleveland, OH 44195, USA
    J Clin Microbiol 42:5739-44. 2004
    ..isolates, which contained 41 isolates of staphylococci representing at least nine species, and 100 positive blood cultures that contained gram-positive cocci in clusters were tested...
  85. pmc Defining blood processing parameters for optimal detection of cryopreserved antigen-specific responses for HIV vaccine trials
    Marta Bull
    Children s Hospital and Regional Medical Center, Seattle, WA, USA
    J Immunol Methods 322:57-69. 2007
    ..routinely employed in clinical HIV vaccine trials to identify antigen-specific T cells in cryopreserved peripheral blood mononuclear cells (PBMC)...
  86. ncbi Proinflammatory cytokine and chemokine modulation by Streptococcus suis in a whole-blood culture system
    Mariela Segura
    Canadian Research Network on Bacterial Pathogens of Swine, Faculte de Medecine Veterinaire, Universite de Montreal, St Hyacinthe, QC, Canada
    FEMS Immunol Med Microbiol 47:92-106. 2006
    ..In this work, using a porcine whole blood model, S...
  87. ncbi Staphylococcus pseudolugdunensis sp. nov., a pyrrolidonyl arylamidase/ornithine decarboxylase-positive bacterium isolated from blood cultures
    Yi Wei Tang
    Department of Pathology, Vanderbilt University Medical Center, Nashville, TN 37232, USA
    Diagn Microbiol Infect Dis 60:351-9. 2008
    Twelve coagulase-negative staphylococcal isolates recovered from blood cultures with positive pyrrolidonyl arylamidase and ornithine decarboxylase reactions were identified as Staphylococcus lugdunensis by the clinical microbiology ..
  88. ncbi Differences in biofilm development and antibiotic susceptibility among Streptococcus pneumoniae isolates from cystic fibrosis samples and blood cultures
    María García-Castillo
    Servicio de Microbiologia, Hospital Universitario Ramón y Cajal Ctra Colmenar Km 9 1, Madrid 28034, Spain
    J Antimicrob Chemother 59:301-4. 2007
    ..between Streptococcus pneumoniae isolates from cystic fibrosis (CF) respiratory samples and those from non-CF blood cultures...
  89. ncbi Recovery of genomic DNA from residual frozen archival blood clots suitable for amplification and use in genotyping assays
    David M Iovannisci
    Children s Hospital and Research Center Oakland, Oakland, California 94609, USA
    Genet Test 10:44-9. 2006
    A greatly neglected source of DNA potentially useful for genetic or forensic studies is the clot remaining from blood samples collected for serum chemistry measurements...
  90. ncbi Adenine nucleotide metabolism in human blood--important roles for leukocytes and erythrocytes
    S Heptinstall
    Centre for Integrated Systems Biology and Medicine, Institute of Clinical Research, University of Nottingham, UK
    J Thromb Haemost 3:2331-9. 2005
    Adenosine diphosphate (ADP) released into blood induces platelet aggregation and contributes to hemostasis and thrombosis...
  91. ncbi Comparison of direct disk diffusion and standard microtitre broth dilution susceptibility testing of blood culture isolates
    Andreas Edelmann
    Institute of Medical Microbiology and Hygiene, University Hospital of Ulm, Ulm, Germany
    J Med Microbiol 56:202-7. 2007
    ..The accuracy of direct disk diffusion susceptibility testing of positive blood cultures was investigated, including for the first time beta-lactam/beta-lactam-inhibitor combination antibiotics...
  92. pmc Multicenter evaluation of the Candida albicans/Candida glabrata peptide nucleic acid fluorescent in situ hybridization method for simultaneous dual-color identification of C. albicans and C. glabrata directly from blood culture bottles
    Janeen R Shepard
    AdvanDx, Inc, 10A Roessler Road, Woburn, MA 01801, USA
    J Clin Microbiol 46:50-5. 2008
    ..albicans and C. glabrata, in a multicenter study. The assay is designed for use directly from positive blood culture bottles in a FISH format. Intact, fixed cells are labeled fluorescent green (C...
  93. pmc Characterization of a juvenile hormone-regulated chymotrypsin-like serine protease gene in Aedes aegypti mosquito
    Guowu Bian
    Department of Biochemistry, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA
    Insect Biochem Mol Biol 38:190-200. 2008
    After female mosquitoes ingest blood from vertebrate hosts, exopeptidases and endopeptidases are required for digesting blood proteins in the midgut into amino acids, which female mosquitoes use to build yolk proteins...
  94. ncbi Blood kinetics of four intraperitoneally administered therapeutic candidate bacteriophages in healthy and neutropenic mice
    Jumpei Uchiyama
    Department of Pediatrics, Faculty of Medicine, Kochi University, Nankoku City, Kochi 783 8505, Japan
    Microbiol Immunol 53:301-4. 2009
    ..Although most animal experiments focus on therapeutic efficacy, the blood clearance kinetics of phages have not been well described...
  95. ncbi Usefulness of real-time PCR for lytA, ply, and Spn9802 on plasma samples for the diagnosis of pneumococcal pneumonia
    G Abdeldaim
    Department of Clinical Microbiology, Uppsala University Hospital, Uppsala, Sweden
    Clin Microbiol Infect 16:1135-41. 2010
    ..In a prospective study of CAP aetiology, an EDTA plasma sample was collected together with blood culture in 92 adult CAP patients and 91 adult controls...
  96. ncbi Transmissibility of hepatitis B virus (HBV) infection through blood transfusion from blood donors with occult HBV infection
    Man Fung Yuen
    Department of Medicine, The University of Hong Kong, Queen Mary Hospital, Hong Kong
    Clin Infect Dis 52:624-32. 2011
    Studies of the transmissibility of hepatitis B virus (HBV) in occult hepatitis B (OHB) through blood transfusion are scarce...
  97. pmc Application of the indirect fluorescent antibody assay in the study of malaria infection in the Yangtze River Three Gorges Reservoir, China
    Wang Duo-Quan
    National Institute of Parasitic Disease, Chinese Center for Disease Control and Prevention, WHO Collaborating Center for Malaria, Schistosomiasis and Filariasis, Shanghai 200025, PR China
    Malar J 8:199. 2009
    ..The objective of this study was to get a reliable baseline on malaria infection in the Yangtze River Three Gorges reservoir area and to provide reference data for future studies about the impact of the project on malaria epidemics...
  98. pmc Rapid decline in the efficiency of HIV drug resistance genotyping from dried blood spots (DBS) and dried plasma spots (DPS) stored at 37 degrees C and high humidity
    J Gerardo Garcia-Lerma
    Laboratory Branch, Division of HIV AIDS Prevention, National Center for HIV AIDS, Viral Hepatitis, STD and TB Prevention, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
    J Antimicrob Chemother 64:33-6. 2009
    Dried blood spots (DBS) and dried plasma spots (DPS) are considered convenient alternatives to serum and plasma for HIV drug resistance testing in resource-limited settings...
  99. ncbi Development of the blood-nerve barrier in neonatal rats
    C E Smith
    Department of Surgery, Division of Plastic and Reconstructive Surgery, Washington University School of Medicine, St. Louis, Missouri, USA
    Microsurgery 21:290-7. 2001
    The blood-nerve barrier (BNB) is constituted by the perineurium and the endothelium of endoneurial microvessels...
  100. pmc Diagnostic comparison of malaria infection in peripheral blood, placental blood and placental biopsies in Cameroonian parturient women
    Judith K Anchang-Kimbi
    Department of Plant and Animal Sciences, University of Buea, Buea, Cameroon
    Malar J 8:126. 2009
    ..The epidemiological and clinical relevance of the different types of malaria diagnosis as well as risk factors associated with malaria infection at delivery were investigated...
  101. pmc Optimization and limitations of use of cryopreserved peripheral blood mononuclear cells for functional and phenotypic T-cell characterization
    Adriana Weinberg
    University of Colorado Denver School of Medicine, Denver, CO, USA
    Clin Vaccine Immunol 16:1176-86. 2009
    The goals of this study were to optimize processing methods of cryopreserved peripheral blood mononuclear cells (PBMC) for immunological assays, identify acceptance parameters for the use of cryopreserved PBMC for functional and ..

Research Grants71

  1. Emotional Mechanisms /Blood-Injection-Injury Phobia
    Olubunmi Olatunji; Fiscal Year: 2004
    The goal of the proposed research is to increase the understanding of the role of fear and disgust in Blood-Injection-Injury (BII) phobia. BII phobia has typically been conceptualized as being mediated by fear...
  2. HIV Treatment Adherence/Risk Reduction Integrated
    Seth Kalichman; Fiscal Year: 2009
    ..If shown effective, the intervention model will have implications for community and clinical services for people living with HIV-AIDS. ..
  3. Fluid Movement across Conjunctiva: Stimulation and Regulation
    OSCAR CANDIA; Fiscal Year: 2007
    ..These studies may provide the groundwork for the design of future treatment modalities for dry-eye syndromes. ..
    Seth Kalichman; Fiscal Year: 2002
    ..Results of the proposed research will provide critical information about improving HIV treatment adherence with low-literacy populations that is needed prior to conducting larger and more expensive clinical trials. ..
  5. Brief HIV Prevention Counseling in South Africa
    Seth Kalichman; Fiscal Year: 2009
    ..Results of this research will therefore test a clinic-based HIV prevention intervention model that is urgently needed in South Africa. ..
  6. Topical Antibiotics to Prevent Lyme Disease
    Gary Wormser; Fiscal Year: 2007
    ..In addition, doxycycline is only recommended for patients bitten by I. scapularis ticks that are engorged with blood, an assessment that requires skill and can only be done if the tick has been saved and is relatively intact...
  7. Mechanisms of HIV Transport Across the BBB
    William Banks; Fiscal Year: 2004
    A prime target of HIV is the CNS. To enter the CNS, HIV must negotiate the blood-brain barrier (BBB). Our goal is to determine how HIV crosses the BBB and ultimately to develop therapeutic strategies for blocking passage...
  8. Cerebral Hemorrhage Model: Mechanisms and Treatments
    Kenneth Wagner; Fiscal Year: 2005
    ..Presently, there are no effective treatments. The cellular and molecular mechanisms through which intracerebral blood induces brain injury are poorly understood...
  9. Alcohol and HIV Risk Reduction in South Africa
    Seth Kalichman; Fiscal Year: 2006
    ..Results of the proposed research will provide critical information about the role of alcohol in HIV transmission risks and will offer urgently needed strategies for improving HIV prevention interventions in South Africa. ..
  10. Measurement of Fluid Flow Across the Ocular Ciliary Body
    OSCAR CANDIA; Fiscal Year: 2006
    ..abstract_text> ..
  11. Gender Violence and HIV Risk Reduction in South Africa
    Leickness Simbayi; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  12. Intervention to Close Digital Divide in AIDS Care
    Seth Kalichman; Fiscal Year: 2004
    ..If successful, the experimental intervention could be readily implemented as a community-based program by AIDS Service Organizations, health centers, and community-based Internet access providers. ..
  13. Prion Transport Across the Blood-Brain Barrier
    William Banks; Fiscal Year: 2009
    ..To produce central nervous system (CNS) disease, PrPsc must enter the brain, which requires it negotiate the blood-brain barrier (BBB)...
    OSCAR CANDIA; Fiscal Year: 2002
    ..Based on this model, he will devise protocols to modify and possibly reverse the secretory/absorptive properties of the tissue. ..
    Seth Kalichman; Fiscal Year: 2002
    ..Results of the proposed study will guide the allocation of HIV prevention resources in establishing brief and effective HIV risk reduction counseling services for populations vulnerable to HIV infection. ..
  16. Discharge Planning and Telehealth for Post CABG Elders
    Ruth Kleinpell; Fiscal Year: 2009
  17. Gene Polymorphisms, Preterm Birth, Neonatal Complicatio*
    Steven Witkin; Fiscal Year: 2005
    ..Identification of genetic factors in mothers or their neonates that influence susceptibility to preterm labor and fetal morbidity in different racial groups will aid in development of improved protocols for monitoring and treatment...
  18. Safety Pharmacokinetics & Efficacy of Artemether & Lumefantrine in Pregnant Women
    Mark Mirochnick; Fiscal Year: 2008
    ..Women enrolling in the trial will receive one of the two study drug formulations, followed by serial blood sampling for anti-malaria drug concentrations and protein binding...
  19. Exposure to Blood Among Home Health Care Nurses
    Jack Leiss; Fiscal Year: 2006
    unreadable] DESCRIPTION: The purpose of this study is to reduce blood-borne infections among home health care nurses by preventing occupational blood exposure...
  20. Nutritional Ecology of Adult Anopheles gambiae
    WOODBRIDGE FOSTER; Fiscal Year: 2006
    ..gambiae have two hosts: vascular plants for sugar and mammals for blood. New evidence indicates that plants and mammals may be equally important to population growth, reproduction, and ..
  21. Ehrilchiacidal Mechanisms In Leukocytes
    Yasuko Rikihisa; Fiscal Year: 2009
    ..The results may point to a potential target for new treatment and prevention of HME and HGE. ..
  22. Gene Expression Control by the 1918 Flu in Macaques
    CAROLE BASKIN; Fiscal Year: 2008
    ..Such knowledge may lead to new therapeutic or vaccine strategies, which may serve as vital countermeasures against future influenza outbreaks. [unreadable] [unreadable] [unreadable]..
  23. Oxidative Stress and Survival of Helicobacter pylori
    Robert Maier; Fiscal Year: 2005
  24. Role of Immunization Site in Eliciting Mucosal Immunity
    Peter Anton; Fiscal Year: 2004
    Christopher Power; Fiscal Year: 2004
    ..From these studies, we expect to gain insights into this common neurological complication in terms of lentivirus-induced neurovirulence and pathogenic host responses. ..
    Robert Sherertz; Fiscal Year: 2003
    ..aureus associated with a viral URI, have been shown to cause outbreaks. Understanding the "cloud phenomenon" may facilitate S.aureus outbreak prevention and possibly the transmission of other pathogens, as well. ..
  27. PTS1 Regulation of Brain Met Enk Levels in Alcoholism
    William Banks; Fiscal Year: 2004
    ..of Met-Enk in the brain is determined by a balance of synthesis, release, degradation, and transport across the blood-brain barrier BB by PTS-1, which carries Met-Enk from the brain to the blood...
  28. Mechanisms and clinical effects of microchimerism in transfused trauma patients
    MICHAEL PAUL BUSCH; Fiscal Year: 2010
    ..another, has been described in association with pregnancy, twinning, transplantation, and, most recently, routine blood transfusion...
    Robin Patel; Fiscal Year: 2002
    ..Specific Aim 3: Culture human retrovirus-5. Intraoperative synovial tissue and whole blood specimens from 50 patients with rheumatoid arthritis and 50 patients with osteoarthritis, and synovial tissue ..
    James D McCully; Fiscal Year: 2010
    ..abstract_text> ..
  31. Clinical Predictors of Antiretroviral Failure
    Daniel Benjamin; Fiscal Year: 2003
    ..abstract_text> ..
  32. Molecular epidemiologic identification of pneumococcal tissue specific genes
    MELINDA MARY PETTIGREW; Fiscal Year: 2010
    ..A greater understanding of the genetic factors that influence the virulence potential of pneumococcal clones is critical to our ability to develop effective methods to control, prevent, and treat these important infections. ..
  33. Virulence Determinants in Helicobacter hepaticus
    Robert Maier; Fiscal Year: 2004
    ..If specific mutants can be created, then virulence determinants can begin to be systematically studied. ..
    ROBERT FLOYD; Fiscal Year: 2004
    ..Additionally, new MRI technology will be developed for assessing tissue localization of specific flee radicals with a focus on nitric oxide intermediates, and tissue-specific contrast enhancement. ..
    Halina Offner; Fiscal Year: 2010
    ..E2 in relapsing, chronic, and spontaneous models of EAE. These studies will clearly establish E2- and EE- dependent pathways of EAE protection and therapy that may have direct relevance for future clinical trials in MS patients. ..
    Thomas Russo; Fiscal Year: 2004
    ..g. hemolysin) and bacterially induced host response elements in directly mediating the pathogenesis of lung injury. ..
    Christopher Power; Fiscal Year: 2010
    ..Many of the scientific techniques and models to be employed in the experiments described herein are unique to the laboratories of this team of investigators who have worked together for many years. ..
  38. The malignant stem cell in multiple myeloma
    Linda Pilarski; Fiscal Year: 2004
    ..Specific aims are: 1. To characterize pre-switch clonotypic MM cells in sorted B lineage subsets from blood and via laser capture of physically, morphologically and/or phenotypically identified BM cells together...
  39. Comparison of Human Ehrlichiosis Agent Genomes
    Yasuko Rikihisa; Fiscal Year: 2010
    ..chaffeensis virulence determinants and their pathogenic mechanisms. The results may point to potential chemotherapy, chemopreventive and/or vaccine candidates for treatment and prevention of human ehrlichiosis. ..
    James Hargrove; Fiscal Year: 2003
    ..Lastly, a panel will discuss the use of modeling in undergraduate and graduate education, and will make recommendations for appropriate activities of a new Research Interest Section on Mathematical Modeling. ..
    Loren Fast; Fiscal Year: 2002
    Transfusion of blood products has been shown to impact a number of the recipient immune responses...
  42. Pathogenesis of leukoencephalopathies in HIV+ patients
    Pasquale Ferrante; Fiscal Year: 2009
    CHRISTOPHER PLOWE; Fiscal Year: 2002
    ..abstract_text> ..
  44. Susceptibility Testing, Treatment Choices, and Outcomes of MDR-GNB Infections
    Lisa Maragakis; Fiscal Year: 2009
    ..This research is designed to better understand how providers use information from various microbiology laboratory testing methods to choose treatments and to identify strategies that lead to the best patient outcomes. ..
  45. Point-of-Care Microfluidics in Lung Cancer
    Mehmet Toner; Fiscal Year: 2009
    ..and the peripheral metastases has been established in the form of circulating tumor cells (CTCs) in peripheral blood, a major challenge remains in deciphering the biology of this very unique cellular population, and ultimately, ..
  46. 14th International Society for Prenatal Diagnosis Meeting
    DIANA BIANCHI; Fiscal Year: 2008
    ..fetal cardiac therapy, population screening for single gene disorders, and cell-free nucleic acids in maternal blood. As a result of attending the ISPD conference participants will be exposed to a wide-range of timely topics that ..
  47. Opiate Addiction and HIV-1 Induced Release of Cytokines
    William Banks; Fiscal Year: 2006
    ..The blood-brain barrier (BBB) has emerged as a significant source of cytokines, prostaglandins, nitric oxide, and other ..
  48. Antiretroviral Pharmacology/Lactating Mother/Infants
    Mark Mirochnick; Fiscal Year: 2006
    ..This data will be used to develop population models of ZDV, 3TC and NVP pharmacokinetics in maternal blood, breast milk and infant blood and to describe infant systemic ARV exposure from breast milk during maternal ZDV/..
  49. Enhancement of antimicrobics by electrical current
    Robin Patel; Fiscal Year: 2007
    ..This novel but simple resistance reversing strategy may generalize to a wide variety of antimicrobics and microorganisms and a wide variety of biofilm-associated human infections. [unreadable] [unreadable] [unreadable] [unreadable]..
  50. Preoperative chemotherapy for breast cancer
    Lajos Pusztai; Fiscal Year: 2007
    ..We expect that our work will lead to the development of microarray-based clinical tests to personalize chemotherapy selection for an individual with newly diagnosed breast cancer. ..
  51. Preservation of Embryonic Stem Cells in Desiccated State
    Mehmet Toner; Fiscal Year: 2007
    ..In Specific Aim 4, we will investigate the stability of dried mouse ES cells and optimize the desiccation and rehydration conditions most conducive to the differentiation of dry preserved mouse ES cells into hepatocytes. ..
    Yasuko Rikihisa; Fiscal Year: 2004
    ..HGE is caused by infection of peripheral blood granulocytes with an obligate intracellular bacterium, an ehrlichia sp. called an HGE agent...
  53. TCR Genes and HIV-Specific Cytotoxic T-lymphocytes
    Spyros Kalams; Fiscal Year: 2006
    ..4) Determine whether the generation of a diverse TCR repertoire contains populations of high-avidity CTL that come to dominate the population of CTL during chronic infection and mediate control of HIV- 1 replication. ..
  54. Heat Activated Gene Therapy Using Radiomimetic CdtB
    Michael Borrelli; Fiscal Year: 2007
    ..Consequently, complementary therapies that can improve local control for primary and recurrent cancers are needed. The proposed study will test the potential of heat-activated CdtB gene therapy to help satisfy this need. ..
  55. 2008 Summer Bioengineering Conference
    Baruch Lieber; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
    Walter Rocca; Fiscal Year: 2005
    ..These three studies will contribute greatly to understanding the causes and possible prevention of PD by exploring novel hypotheses and by using innovative methods. ..
  57. Gene expression of Borrelia during Babesia coinfection
    James Coleman; Fiscal Year: 2004
    ..burgdorferi and B. microti alone and concurrently. ..
    Walter Rocca; Fiscal Year: 2009
    ..abstract_text> ..
  59. Blood Exposure and Primary Prevention in the Home Care *
    JANE LIPSCOMP; Fiscal Year: 2007
    DESCRIPTION: Exposure to blood and body fluids, most often through the use of conventional unsafe needles that transmit bloodborne infections to health care workers, continues to be the most life threatening risk facing healthcare ..
  60. Synergistic combinations of chimeric anti-SEB: Engineering anti-toxins
    RICHARD GOLDSBY; Fiscal Year: 2008
    ..Chimeric human-mouse forms of the most effective combinations will be engineered to produce antibodies that will be better tolerated in humans. [unreadable] [unreadable] [unreadable]..
  61. Genetic/Immunological Basis of Tuberculosis and Anergy
    Julio Delgado; Fiscal Year: 2005
    ..It is anticipated that these results will be useful in the development of genetic and immune therapeutic strategies to combat tuberculosis. ..
  62. Reconstitution of Protective CMV Immunity and Immune Regulation After HAART
    Adriana Weinberg; Fiscal Year: 2008
    ..The study will be conducted using cryopreserved peripheral blood mononuclear cells (PBMC) and plasma stored by the AIDS Clinical Trials Group...
  63. Workplace Violence Risk in the Home Health Work Place
    Jane Lipscomb; Fiscal Year: 2003
    ..abstract_text> ..
  64. Burden of Malaria in Pregnancy in India
    Davidson Hamer; Fiscal Year: 2007
    ..The information generated by the study will also be instrumental in the development of new programs for preventing and mitigating the impact of MIP in India. [unreadable] [unreadable] [unreadable]..
    Jane Lipscomb; Fiscal Year: 2001
    ..abstract_text> ..
  66. Evaluation of Workplace Violence Prevention Intervention
    Jane Lipscomb; Fiscal Year: 2006
    ..4) Design and implement a violence prevention intervention within these workplaces. 5) Conduct a process and outcome evaluation of the intervention in sample workplaces one year following program implementation. ..
  67. HIV Protease Activation and Viral Replication
    Julie Nelson; Fiscal Year: 2006
    ..II. Characterize the effect of substitutions in the F-IIV protease dimer interface on viral replication. IlI. Characterize HIV protease activation within GagPol as a potential target for inhibition. ..
  68. Regulation of breast cancer growth by activation peptide
    Vaclav Vetvicka; Fiscal Year: 2006
    ..The overall goal is to generate a pharmacological agent for breast cancer based on blockage of the autocrine growth factor activity of pCD. ..