pluripotent stem cells

Summary

Summary: Cells that can give rise to most types of cells but not all types of cells necessary for fetal development. (Stem Cells: A Primer [Internet]. Bethesda (MD): National Institutes of Health (US); 2000 May [cited 2002 Apr 5]. Available from: http://www.nih.gov/news/stemcell/primer.htm)

Top Publications

  1. ncbi Induced pluripotent stem cell lines derived from human somatic cells
    Junying Yu
    Genome Center of Wisconsin, Madison, WI 53706 1580, USA
    Science 318:1917-20. 2007
  2. ncbi Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors
    Kazutoshi Takahashi
    Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
    Cell 126:663-76. 2006
  3. ncbi Induction of pluripotent stem cells from adult human fibroblasts by defined factors
    Kazutoshi Takahashi
    Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
    Cell 131:861-72. 2007
  4. ncbi The ground state of embryonic stem cell self-renewal
    Qi Long Ying
    Center for Stem Cell and Regenerative Medicine, Department of Cell and Neurobiology, Keck School of Medicine, University of Southern California, 1501 San Pablo Street, ZNI 529, Los Angeles, California 90033, USA
    Nature 453:519-23. 2008
  5. pmc Genome-wide maps of chromatin state in pluripotent and lineage-committed cells
    Tarjei S Mikkelsen
    Broad Institute of Harvard and MIT, Cambridge, Massachusetts 02142, USA
    Nature 448:553-60. 2007
  6. ncbi Functional expression cloning of Nanog, a pluripotency sustaining factor in embryonic stem cells
    Ian Chambers
    Institute for Stem Cell Research, University of Edinburgh, King s Buildings, West Mains Road, Edinburgh EH9 3JQ, Scotland
    Cell 113:643-55. 2003
  7. pmc Highly efficient neural conversion of human ES and iPS cells by dual inhibition of SMAD signaling
    Stuart M Chambers
    Developmental Biology Program, Sloan Kettering Institute, 1275 York Ave, New York, New York 10065, USA
    Nat Biotechnol 27:275-80. 2009
  8. ncbi Nanog safeguards pluripotency and mediates germline development
    Ian Chambers
    MRC Centre Development in Stem Cell Biology, Institute for Stem Cell Research, School of Biological Sciences, University of Edinburgh, King s Buildings, West Mains Road, Edinburgh EH9 3JQ, UK
    Nature 450:1230-4. 2007
  9. ncbi Reprogramming of human somatic cells to pluripotency with defined factors
    In Hyun Park
    Division of Pediatric Hematology Oncology, Children s Hospital Boston and Dana Farber Cancer Institute, Boston, Massachusetts 02115, USA
    Nature 451:141-6. 2008
  10. pmc Genome-scale DNA methylation maps of pluripotent and differentiated cells
    Alexander Meissner
    Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, Massachusetts 02142, USA
    Nature 454:766-70. 2008

Detail Information

Publications374 found, 100 shown here

  1. ncbi Induced pluripotent stem cell lines derived from human somatic cells
    Junying Yu
    Genome Center of Wisconsin, Madison, WI 53706 1580, USA
    Science 318:1917-20. 2007
    ..show that four factors (OCT4, SOX2, NANOG, and LIN28) are sufficient to reprogram human somatic cells to pluripotent stem cells that exhibit the essential characteristics of embryonic stem (ES) cells...
  2. ncbi Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors
    Kazutoshi Takahashi
    Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
    Cell 126:663-76. 2006
    ..Little is known about factors that induce this reprogramming. Here, we demonstrate induction of pluripotent stem cells from mouse embryonic or adult fibroblasts by introducing four factors, Oct3/4, Sox2, c-Myc, and Klf4, ..
  3. ncbi Induction of pluripotent stem cells from adult human fibroblasts by defined factors
    Kazutoshi Takahashi
    Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
    Cell 131:861-72. 2007
    ..Furthermore, these cells could differentiate into cell types of the three germ layers in vitro and in teratomas. These findings demonstrate that iPS cells can be generated from adult human fibroblasts...
  4. ncbi The ground state of embryonic stem cell self-renewal
    Qi Long Ying
    Center for Stem Cell and Regenerative Medicine, Department of Cell and Neurobiology, Keck School of Medicine, University of Southern California, 1501 San Pablo Street, ZNI 529, Los Angeles, California 90033, USA
    Nature 453:519-23. 2008
    ..This property may account for their latent tumorigenicity. The delineation of minimal requirements for self-renewal now provides a defined platform for the precise description and dissection of the pluripotent state...
  5. pmc Genome-wide maps of chromatin state in pluripotent and lineage-committed cells
    Tarjei S Mikkelsen
    Broad Institute of Harvard and MIT, Cambridge, Massachusetts 02142, USA
    Nature 448:553-60. 2007
    ..This study provides a framework for the application of comprehensive chromatin profiling towards characterization of diverse mammalian cell populations...
  6. ncbi Functional expression cloning of Nanog, a pluripotency sustaining factor in embryonic stem cells
    Ian Chambers
    Institute for Stem Cell Research, University of Edinburgh, King s Buildings, West Mains Road, Edinburgh EH9 3JQ, Scotland
    Cell 113:643-55. 2003
    ..These findings establish a central role for Nanog in the transcription factor hierarchy that defines ES cell identity...
  7. pmc Highly efficient neural conversion of human ES and iPS cells by dual inhibition of SMAD signaling
    Stuart M Chambers
    Developmental Biology Program, Sloan Kettering Institute, 1275 York Ave, New York, New York 10065, USA
    Nat Biotechnol 27:275-80. 2009
    ..Noggin/SB431542-based neural induction should facilitate the use of hES and hiPS cells in regenerative medicine and disease modeling and obviate the need for protocols based on stromal feeders or embryoid bodies...
  8. ncbi Nanog safeguards pluripotency and mediates germline development
    Ian Chambers
    MRC Centre Development in Stem Cell Biology, Institute for Stem Cell Research, School of Biological Sciences, University of Edinburgh, King s Buildings, West Mains Road, Edinburgh EH9 3JQ, UK
    Nature 450:1230-4. 2007
    ..We surmise that Nanog stabilizes embryonic stem cells in culture by resisting or reversing alternative gene expression states...
  9. ncbi Reprogramming of human somatic cells to pluripotency with defined factors
    In Hyun Park
    Division of Pediatric Hematology Oncology, Children s Hospital Boston and Dana Farber Cancer Institute, Boston, Massachusetts 02115, USA
    Nature 451:141-6. 2008
    ..These data demonstrate that defined factors can reprogramme human cells to pluripotency, and establish a method whereby patient-specific cells might be established in culture...
  10. pmc Genome-scale DNA methylation maps of pluripotent and differentiated cells
    Alexander Meissner
    Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, Massachusetts 02142, USA
    Nature 454:766-70. 2008
    ..More generally, the results establish reduced representation bisulphite sequencing as a powerful technology for epigenetic profiling of cell populations relevant to developmental biology, cancer and regenerative medicine...
  11. ncbi The homeoprotein Nanog is required for maintenance of pluripotency in mouse epiblast and ES cells
    Kaoru Mitsui
    Laboratory of Animal Molecular Technology, Research and Education Center for Genetic Information, Nara Institute of Science and Technology, Nara 630 0192, Japan
    Cell 113:631-42. 2003
    ..nanog-deficient ES cells lost pluripotency and differentiated into extraembryonic endoderm lineage. These data demonstrate that Nanog is a critical factor underlying pluripotency in both ICM and ES cells...
  12. pmc Human induced pluripotent stem cells free of vector and transgene sequences
    Junying Yu
    Morgridge Institute for Research, Madison, WI 53707 7365, USA
    Science 324:797-801. 2009
    ..These results demonstrate that reprogramming human somatic cells does not require genomic integration or the continued presence of exogenous reprogramming factors and removes one obstacle to the clinical application of human iPS cells...
  13. pmc Control of the embryonic stem cell state
    Richard A Young
    Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA
    Cell 144:940-54. 2011
    Embryonic stem cells and induced pluripotent stem cells hold great promise for regenerative medicine. These cells can be propagated in culture in an undifferentiated state but can be induced to differentiate into specialized cell types...
  14. ncbi Derivation of pluripotent epiblast stem cells from mammalian embryos
    I Gabrielle M Brons
    Department of Surgery and Cambridge Institute for Medical Research, Addenbrooke s Hospital, University of Cambridge, Cambridge CB2 0XY, UK
    Nature 448:191-5. 2007
    ..Here we show that pluripotent stem cells can be derived from the late epiblast layer of post-implantation mouse and rat embryos using chemically ..
  15. ncbi New cell lines from mouse epiblast share defining features with human embryonic stem cells
    Paul J Tesar
    Laboratory of Molecular Biology, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland 20892, USA
    Nature 448:196-9. 2007
    ..These results show that epiblast cells can be maintained as stable cell lines and interrogated to understand how pluripotent cells generate distinct fates during early development...
  16. pmc Parkinson's disease patient-derived induced pluripotent stem cells free of viral reprogramming factors
    Frank Soldner
    The Whitehead Institute, Cambridge Center, MA 02142, USA
    Cell 136:964-77. 2009
    Induced pluripotent stem cells (iPSCs) derived from somatic cells of patients represent a powerful tool for biomedical research and may provide a source for replacement therapies...
  17. ncbi Generation of induced pluripotent stem cells without Myc from mouse and human fibroblasts
    Masato Nakagawa
    Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
    Nat Biotechnol 26:101-6. 2008
    Direct reprogramming of somatic cells provides an opportunity to generate patient- or disease-specific pluripotent stem cells. Such induced pluripotent stem (iPS) cells were generated from mouse fibroblasts by retroviral transduction of ..
  18. pmc Stem cells, the molecular circuitry of pluripotency and nuclear reprogramming
    Rudolf Jaenisch
    Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, MA 02142, USA
    Cell 132:567-82. 2008
    ....
  19. pmc Induced pluripotent stem cells from a spinal muscular atrophy patient
    Allison D Ebert
    The Waisman Center, The Stem Cell and Regenerative Medicine Center, University of Wisconsin Madison, 1500 Highland Avenue, Madison, Wisconsin 53705, USA
    Nature 457:277-80. 2009
    ..Here we report the generation of induced pluripotent stem cells from skin fibroblast samples taken from a child with spinal muscular atrophy...
  20. ncbi Induced pluripotent stem cells generated from patients with ALS can be differentiated into motor neurons
    John T Dimos
    Harvard Stem Cell Institute, Stowers Medical Institute, Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA 02138, USA
    Science 321:1218-21. 2008
    The generation of pluripotent stem cells from an individual patient would enable the large-scale production of the cell types affected by that patient's disease...
  21. ncbi Naive and primed pluripotent states
    Jennifer Nichols
    Wellcome Trust Centre for Stem Cell Research, Department of Physiology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK
    Cell Stem Cell 4:487-92. 2009
    ..We propose that two phases of pluripotency can be defined: naive and primed. This distinction extends to pluripotent stem cells derived from embryos or by molecular reprogramming ex vivo.
  22. ncbi Maintenance of pluripotency in human and mouse embryonic stem cells through activation of Wnt signaling by a pharmacological GSK-3-specific inhibitor
    Noboru Sato
    Laboratory of Molecular Vertebrate Embryology, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
    Nat Med 10:55-63. 2004
    ..These results suggest that the use of GSK-3-specific inhibitors such as BIO may have practical applications in regenerative medicine...
  23. ncbi Generation of germline-competent induced pluripotent stem cells
    Keisuke Okita
    Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
    Nature 448:313-7. 2007
    We have previously shown that pluripotent stem cells can be induced from mouse fibroblasts by retroviral introduction of Oct3/4 (also called Pou5f1), Sox2, c-Myc and Klf4, and subsequent selection for Fbx15 (also called Fbxo15) expression...
  24. ncbi A parallel circuit of LIF signalling pathways maintains pluripotency of mouse ES cells
    Hitoshi Niwa
    Laboratory for Pluripotent Cell Studies, RIKEN Center for Developmental Biology, 2 2 3 Minatojima minamimachi, Chuo Ku, Kobe 6500047, Japan
    Nature 460:118-22. 2009
    ....
  25. pmc Inhibition of glycogen synthase kinase-3 alleviates Tcf3 repression of the pluripotency network and increases embryonic stem cell resistance to differentiation
    Jason Wray
    Wellcome Trust Centre for Stem Cell Research and Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK
    Nat Cell Biol 13:838-45. 2011
    ..We conclude that Gsk3 inhibition stabilizes the embryonic stem cell state primarily by reducing repressive influence on the core pluripotency network...
  26. pmc Virus-free induction of pluripotency and subsequent excision of reprogramming factors
    Keisuke Kaji
    MRC Centre for Regenerative Medicine, Institute for Stem Cell Research, University of Edinburgh, Edinburgh EH9 3JQ, UK
    Nature 458:771-5. 2009
    ....
  27. pmc Dynamic changes in the copy number of pluripotency and cell proliferation genes in human ESCs and iPSCs during reprogramming and time in culture
    Louise C Laurent
    Department of Reproductive Medicine, University of California, San Diego, La Jolla, CA 92093, USA
    Cell Stem Cell 8:106-18. 2011
    Genomic stability is critical for the clinical use of human embryonic and induced pluripotent stem cells. We performed high-resolution SNP (single-nucleotide polymorphism) analysis on 186 pluripotent and 119 nonpluripotent samples...
  28. pmc Highly efficient miRNA-mediated reprogramming of mouse and human somatic cells to pluripotency
    Frederick Anokye-Danso
    Department of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
    Cell Stem Cell 8:376-88. 2011
    ..Thus, our data show that miRNA and Hdac-mediated pathways can cooperate in a powerful way to reprogram somatic cells to pluripotency...
  29. pmc Modelling pathogenesis and treatment of familial dysautonomia using patient-specific iPSCs
    Gabsang Lee
    Developmental Biology Program, Sloan Kettering Institute, 1275 York Ave, USA
    Nature 461:402-6. 2009
    The isolation of human induced pluripotent stem cells (iPSCs) offers a new strategy for modelling human disease. Recent studies have reported the derivation and differentiation of disease-specific human iPSCs...
  30. ncbi Generation of induced pluripotent stem cells using recombinant proteins
    Hongyan Zhou
    Department of Chemistry, The Scripps Research Institute, La Jolla, CA 92037, USA
    Cell Stem Cell 4:381-4. 2009
  31. pmc Robust cardiomyocyte differentiation from human pluripotent stem cells via temporal modulation of canonical Wnt signaling
    Xiaojun Lian
    Department of Chemical and Biological Engineering, University of Wisconsin, Madison, WI 53706, USA
    Proc Natl Acad Sci U S A 109:E1848-57. 2012
    Human pluripotent stem cells (hPSCs) offer the potential to generate large numbers of functional cardiomyocytes from clonal and patient-specific cell sources...
  32. pmc Regulated fluctuations in nanog expression mediate cell fate decisions in embryonic stem cells
    Tibor Kalmar
    Department of Genetics, University of Cambridge, Cambridge, United Kingdom
    PLoS Biol 7:e1000149. 2009
    ....
  33. pmc Induced pluripotent stem cells and embryonic stem cells are distinguished by gene expression signatures
    Mark H Chin
    Department of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA
    Cell Stem Cell 5:111-23. 2009
    Induced pluripotent stem cells (iPSCs) outwardly appear to be indistinguishable from embryonic stem cells (ESCs)...
  34. pmc Dissecting direct reprogramming through integrative genomic analysis
    Tarjei S Mikkelsen
    Broad Institute of MIT and Harvard, 7 Cambridge Center, Cambridge, Massachusetts 02142, USA
    Nature 454:49-55. 2008
    ..We demonstrate that RNA inhibition of transcription factors can facilitate reprogramming, and that treatment with DNA methyltransferase inhibitors can improve the overall efficiency of the reprogramming process...
  35. ncbi MicroRNA-145 regulates OCT4, SOX2, and KLF4 and represses pluripotency in human embryonic stem cells
    Na Xu
    Neuroscience Research Institute, Department of Molecular, Cellular, and Developmental Biology, The University of California, Santa Barbara, Santa Barbara, CA 93106, USA
    Cell 137:647-58. 2009
    ..This work reveals a direct link between the core reprogramming factors and miR-145 and uncovers a double-negative feedback loop involving OCT4, SOX2, KLF4, and miR-145...
  36. ncbi Stage-specific optimization of activin/nodal and BMP signaling promotes cardiac differentiation of mouse and human pluripotent stem cell lines
    Steven J Kattman
    McEwen Center for Regenerative Medicine, University Health Network, Toronto, Ontario, Canada
    Cell Stem Cell 8:228-40. 2011
    Efficient differentiation of embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) to a variety of lineages requires step-wise approaches replicating the key commitment stages found during embryonic development...
  37. pmc Pluripotency and cellular reprogramming: facts, hypotheses, unresolved issues
    Jacob H Hanna
    The Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Cambridge, MA 02142, USA
    Cell 143:508-25. 2010
    Direct reprogramming of somatic cells to induced pluripotent stem cells by ectopic expression of defined transcription factors has raised fundamental questions regarding the epigenetic stability of the differentiated cell state...
  38. ncbi Generation of mouse induced pluripotent stem cells without viral vectors
    Keisuke Okita
    Center for iPS Cell Research and Application CiRA, Institute for Integrated Cell Material Sciences, Kyoto University, Kyoto 606 8507, Japan
    Science 322:949-53. 2008
    ..The production of virus-free iPS cells, albeit from embryonic fibroblasts, addresses a critical safety concern for potential use of iPS cells in regenerative medicine...
  39. pmc Recurrent variations in DNA methylation in human pluripotent stem cells and their differentiated derivatives
    Kristopher L Nazor
    Center for Regenerative Medicine, Department of Chemical Physiology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Cell Stem Cell 10:620-34. 2012
    Human pluripotent stem cells (hPSCs) are potential sources of cells for modeling disease and development, drug discovery, and regenerative medicine...
  40. ncbi Polycomb complexes repress developmental regulators in murine embryonic stem cells
    Laurie A Boyer
    Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, Massachusetts 02142, USA
    Nature 441:349-53. 2006
    ..Our results indicate that dynamic repression of developmental pathways by Polycomb complexes may be required for maintaining ES cell pluripotency and plasticity during embryonic development...
  41. ncbi In vitro reprogramming of fibroblasts into a pluripotent ES-cell-like state
    Marius Wernig
    Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02142, USA
    Nature 448:318-24. 2007
    ..Our results show that the biological potency and epigenetic state of in-vitro-reprogrammed induced pluripotent stem cells are indistinguishable from those of ES cells.
  42. pmc Induced pluripotent stem cells generated without viral integration
    Matthias Stadtfeld
    Massachusetts General Hospital Cancer Center and Center for Regenerative Medicine, 185 Cambridge Street, Boston, MA 02114, USA
    Science 322:945-9. 2008
    b>Pluripotent stem cells have been generated from mouse and human somatic cells by viral expression of the transcription factors Oct4, Sox2, Klf4, and c-Myc...
  43. ncbi Lineage-specific polycomb targets and de novo DNA methylation define restriction and potential of neuronal progenitors
    Fabio Mohn
    Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, 4058 Basel, Switzerland
    Mol Cell 30:755-66. 2008
    ..These data suggest a model how de novo DNA methylation and dynamic switches in Polycomb targets restrict pluripotency and define the developmental potential of progenitor cells...
  44. ncbi Interaction between Oct3/4 and Cdx2 determines trophectoderm differentiation
    Hitoshi Niwa
    Laboratory for Pluripotent Cell Studies, RIKEN Center for Developmental Biology CDB, 2 2 3 Minatojima minamimachi, Kobe, Hyogo 650 0047, Japan
    Cell 123:917-29. 2005
    ..This suggests that reciprocal inhibition between lineage-specific transcription factors might be involved in the first differentiation event of mammalian development...
  45. ncbi Adult mice generated from induced pluripotent stem cells
    Michael J Boland
    Department of Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Nature 461:91-4. 2009
    ....
  46. pmc Aberrant silencing of imprinted genes on chromosome 12qF1 in mouse induced pluripotent stem cells
    Matthias Stadtfeld
    Howard Hughes Medical Institute at Massachusetts General Hospital, Center for Regenerative Medicine Harvard Stem Cell Institute, 185 Cambridge Street, Boston, Massachusetts 02114, USA
    Nature 465:175-81. 2010
    Induced pluripotent stem cells (iPSCs) have been generated by enforced expression of defined sets of transcription factors in somatic cells...
  47. ncbi Reconstitution of the mouse germ cell specification pathway in culture by pluripotent stem cells
    Katsuhiko Hayashi
    Department of Anatomy and Cell Biology, Graduate School of Medicine, Kyoto University, Yoshida konoe cho, Sakyo ku, Kyoto 606 8501, Japan
    Cell 146:519-32. 2011
    ..PGCLCs were generated from embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) through epiblast-like cells (EpiLCs), a cellular state highly similar to pregastrulating epiblasts ..
  48. pmc Promotion of reprogramming to ground state pluripotency by signal inhibition
    Jose Silva
    Wellcome Trust Centre for Stem Cell Research, University of Cambridge, Cambridge, United Kingdom
    PLoS Biol 6:e253. 2008
    ..We therefore applied molecularly defined conditions for the derivation and propagation of authentic pluripotent stem cells from embryos...
  49. pmc Direct cell reprogramming is a stochastic process amenable to acceleration
    Jacob Hanna
    The Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, USA
    Nature 462:595-601. 2009
    ....
  50. pmc Modelling schizophrenia using human induced pluripotent stem cells
    Kristen J Brennand
    Salk Institute for Biological Studies, Laboratory of Genetics, 10010 North Torrey Pines Road, La Jolla California 92037, USA
    Nature 473:221-5. 2011
    ..and molecular defects of SCZD, we directly reprogrammed fibroblasts from SCZD patients into human induced pluripotent stem cells (hiPSCs) and subsequently differentiated these disorder-specific hiPSCs into neurons (Supplementary Fig...
  51. ncbi The ground state of pluripotency
    Jason Wray
    Wellcome Trust Centre for Stem Cell Research, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK
    Biochem Soc Trans 38:1027-32. 2010
    ..The stability of this state is reflected in the homogeneity of ES cell populations cultured in the presence of small-molecule inhibitors of MEK (mitogen-activated protein kinase/ERK kinase) and GSK3...
  52. pmc Generation of isogenic pluripotent stem cells differing exclusively at two early onset Parkinson point mutations
    Frank Soldner
    The Whitehead Institute, 9 Cambridge Center, Cambridge, MA 02142, USA
    Cell 146:318-31. 2011
    Patient-specific induced pluripotent stem cells (iPSCs) derived from somatic cells provide a unique tool for the study of human disease, as well as a promising source for cell replacement therapies...
  53. ncbi Induction of pluripotent stem cells from fibroblast cultures
    Kazutoshi Takahashi
    Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara machi, Shogoin, Sakyo ku, Kyoto 606 8507, Japan
    Nat Protoc 2:3081-9. 2007
    ..One way to circumvent these issues is to generate pluripotent stem cells directly from somatic cells...
  54. pmc Self-renewing endodermal progenitor lines generated from human pluripotent stem cells
    Xin Cheng
    Department of Pathology and Laboratory Medicine, Children s Hospital of Philadelphia, PA 19104, USA
    Cell Stem Cell 10:371-84. 2012
    The use of human pluripotent stem cells for laboratory studies and cell-based therapies is hampered by their tumor-forming potential and limited ability to generate pure populations of differentiated cell types in vitro...
  55. pmc Neural differentiation of human induced pluripotent stem cells follows developmental principles but with variable potency
    Bao Yang Hu
    Department of Anatomy, School of Medicine and Public Health, University of Wisconsin, Waisman Center, Madison, WI 53705, USA
    Proc Natl Acad Sci U S A 107:4335-40. 2010
    For the promise of human induced pluripotent stem cells (iPSCs) to be realized, it is necessary to ask if and how efficiently they may be differentiated to functional cells of various lineages...
  56. ncbi Pluripotency factors in embryonic stem cells regulate differentiation into germ layers
    Matt Thomson
    FAS Center for Systems Biology, Harvard University, Cambridge, MA 02138, USA
    Cell 145:875-89. 2011
    ..Our study provides a framework for understanding how complex transcription factor networks control cell fate decisions in progenitor cells...
  57. pmc Clonogenic neoblasts are pluripotent adult stem cells that underlie planarian regeneration
    Daniel E Wagner
    Howard Hughes Medical Institute, Department of Biology, Massachusetts Institute of Technology MIT, Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, MA 02142, USA
    Science 332:811-6. 2011
    ..Single transplanted cNeoblasts restored regeneration in lethally irradiated hosts. We conclude that broadly distributed, adult pluripotent stem cells underlie the remarkable regenerative abilities of planarians.
  58. pmc Human embryonic stem cells with biological and epigenetic characteristics similar to those of mouse ESCs
    Jacob Hanna
    The Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA
    Proc Natl Acad Sci U S A 107:9222-7. 2010
    ..The generation of validated "naïve" human ESCs will allow the molecular dissection of a previously undefined pluripotent state in humans and may open up new opportunities for patient-specific, disease-relevant research...
  59. pmc Extracellular matrix promotes highly efficient cardiac differentiation of human pluripotent stem cells: the matrix sandwich method
    Jianhua Zhang
    Department of Medicine, School of Medicine and Public Health, University of Wisconsin, Madison, WI, USA
    Circ Res 111:1125-36. 2012
    Cardiomyocytes (CMs) differentiated from human pluripotent stem cells (PSCs) are increasingly being used for cardiovascular research, including disease modeling, and hold promise for clinical applications...
  60. pmc Embryonic stem cells require Wnt proteins to prevent differentiation to epiblast stem cells
    Derk ten Berge
    Erasmus MC Stem Cell Institute, Department of Cell Biology, Erasmus Medical Center, P O Box 2040, 3000 CA Rotterdam, The Netherlands
    Nat Cell Biol 13:1070-5. 2011
    b>Pluripotent stem cells exist in naive and primed states, epitomized by mouse embryonic stem cells (ESCs) and the developmentally more advanced epiblast stem cells (EpiSCs; ref. 1)...
  61. pmc OCT4/SOX2-independent Nanog autorepression modulates heterogeneous Nanog gene expression in mouse ES cells
    Pablo Navarro
    MRC Centre for Regenerative Medicine, Institute for Stem Cell Research, School of Biological Sciences, University of Edinburgh, Edinburgh, Scotland
    EMBO J 31:4547-62. 2012
    ..Therefore, cellular variability in self-renewal efficiency is an emergent property of the pluripotency gene regulatory network...
  62. pmc Rapid and efficient reprogramming of somatic cells to induced pluripotent stem cells by retinoic acid receptor gamma and liver receptor homolog 1
    Wei Wang
    Wellcome Trust Sanger Institute, Hinxton CB10 1HH, United Kingdom
    Proc Natl Acad Sci U S A 108:18283-8. 2011
    Somatic cells can be reprogrammed to induced pluripotent stem cells (iPSCs) by expressing four transcription factors: Oct4, Sox2, Klf4, and c-Myc...
  63. pmc Somatic oxidative bioenergetics transitions into pluripotency-dependent glycolysis to facilitate nuclear reprogramming
    Clifford D L Folmes
    Center for Regenerative Medicine and Marriott Heart Disease Research Program, Division of Cardiovascular Diseases, Department of Medicine, Mayo Clinic, Rochester, MN 55905, USA
    Cell Metab 14:264-71. 2011
    The bioenergetics of somatic dedifferentiation into induced pluripotent stem cells remains largely unknown. Here, stemness factor-mediated nuclear reprogramming reverted mitochondrial networks into cristae-poor structures...
  64. pmc Incomplete DNA methylation underlies a transcriptional memory of somatic cells in human iPS cells
    Yuki Ohi
    Department of Ob Gyn and Center for Reproductive Sciences, University of California San Francisco, 513 Parnassus Avenue, San Francisco, California 94143, USA
    Nat Cell Biol 13:541-9. 2011
    ..Knockdown of the incompletely reprogrammed gene C9orf64 (chromosome 9 open reading frame 64) reduces the efficiency of human iPS cell generation, indicating that somatic memory genes may be functionally relevant during reprogramming...
  65. pmc The transcriptional foundation of pluripotency
    Ian Chambers
    MRC Centre for Regenerative Medicine, Institute for Stem Cell Research, School of Biological Sciences, University of Edinburgh, King s Buildings, Edinburgh EH9 3JQ, UK
    Development 136:2311-22. 2009
    ..Here we discuss how these observations advance our understanding of the transcription factor network that controls pluripotent identity and highlight unresolved issues that arise from these studies...
  66. pmc Suppression of Erk signalling promotes ground state pluripotency in the mouse embryo
    Jennifer Nichols
    Wellcome Trust Centre for Stem Cell Research, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK
    Development 136:3215-22. 2009
    ..We propose that ES cells are not a tissue culture creation, but are essentially identical to pre-implantation epiblast cells...
  67. pmc Klf4 reverts developmentally programmed restriction of ground state pluripotency
    Ge Guo
    Wellcome Trust Centre for Stem Cell Research, University of Cambridge, Cambridge CB2 1QR, UK
    Development 136:1063-9. 2009
    ..However, because a single transgene is the minimum requirement to attain the ground state, EpiSCs offer an attractive opportunity for screening for unknown components of the reprogramming process...
  68. ncbi Direct reprogramming of human neural stem cells by OCT4
    Jeong Beom Kim
    Max Planck Institute for Molecular Biomedicine, Department of Cell and Developmental Biology, Röntgenstrasse 20, 48149 Munster, NRW, Germany
    Nature 461:649-3. 2009
    ..One-factor iPS cell generation will advance the field further towards understanding reprogramming and generating patient-specific pluripotent stem cells.
  69. pmc Functional cardiomyocytes derived from human induced pluripotent stem cells
    Jianhua Zhang
    Department of Medicine, University of Wisconsin, WiCell Research Institute, Madison, WI 53792 3248, USA
    Circ Res 104:e30-41. 2009
    ..We conclude that human iPS cells can differentiate into functional cardiomyocytes, and thus iPS cells are a viable option as an autologous cell source for cardiac repair and a powerful tool for cardiovascular research...
  70. pmc Modeling early retinal development with human embryonic and induced pluripotent stem cells
    Jason S Meyer
    Stem Cell Research Program, Waisman Center, Departments of Anatomy and Neurology, University of Wisconsin Madison, 1500 Highland Avenue, Madison, WI 53705, USA
    Proc Natl Acad Sci U S A 106:16698-703. 2009
    Human pluripotent stem cells have the potential to provide comprehensive model systems for the earliest stages of human ontogenesis...
  71. pmc Selective blockade of microRNA processing by Lin28
    Srinivas R Viswanathan
    Stem Cell Program, Children s Hospital Boston, Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Harvard Stem Cell Institute, Boston, MA 02115, USA
    Science 320:97-100. 2008
    ..Our results identify Lin28 as a negative regulator of miRNA biogenesis and suggest that Lin28 may play a central role in blocking miRNA-mediated differentiation in stem cells and in certain cancers...
  72. pmc Role of the murine reprogramming factors in the induction of pluripotency
    Rupa Sridharan
    David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA
    Cell 136:364-77. 2009
    ..These data uncover temporal and separable contributions of the four factors during the reprogramming process and indicate that ectopic c-Myc predominantly acts before pluripotency regulators are activated...
  73. pmc Disease-specific induced pluripotent stem cells
    In Hyun Park
    Department of Medicine, Division of Pediatric Hematology Oncology, Children s Hospital Boston, and Dana Farber Cancer Institute, Boston, MA 02115, USA
    Cell 134:877-86. 2008
    ..Such disease-specific stem cells offer an unprecedented opportunity to recapitulate both normal and pathologic human tissue formation in vitro, thereby enabling disease investigation and drug development...
  74. pmc Hyperdynamic plasticity of chromatin proteins in pluripotent embryonic stem cells
    Eran Meshorer
    National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Dev Cell 10:105-16. 2006
    ....
  75. pmc Dynamic equilibrium and heterogeneity of mouse pluripotent stem cells with distinct functional and epigenetic states
    Katsuhiko Hayashi
    Wellcome Trust Cancer Research UK Gurdon Institute, The Henry Wellcome Building of Cancer and Developmental Biology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK
    Cell Stem Cell 3:391-401. 2008
    ....
  76. ncbi The adult Drosophila posterior midgut is maintained by pluripotent stem cells
    Benjamin Ohlstein
    Howard Hughes Medical Institute Research Laboratories, Department of Embryology, Carnegie Institution of Washington, 3520 San Martin Drive, Baltimore, Maryland 21218, USA
    Nature 439:470-4. 2006
    ..The identification of Drosophila intestinal stem cells with striking similarities to their vertebrate counterparts will facilitate the genetic analysis of normal and abnormal intestinal function...
  77. pmc An embryonic stem cell chromatin remodeling complex, esBAF, is an essential component of the core pluripotency transcriptional network
    Lena Ho
    Programs in Immunology, Stanford University, Stanford, CA 94305, USA
    Proc Natl Acad Sci U S A 106:5187-91. 2009
    ....
  78. pmc The developmental dismantling of pluripotency is reversed by ectopic Oct4 expression
    Rodrigo Osorno
    Institute for Stem Cell Research, MRC Centre for Regenerative Medicine, School of Biological Sciences, University of Edinburgh, 5 Little France Drive, Edinburgh EH16 4UU, UK
    Development 139:2288-98. 2012
    ..Our data suggest that decreasing Oct4 expression is converted to a sudden drop in competence to maintain pluripotency gene regulatory network activity that is subsequently stabilized by epigenetic locks...
  79. pmc BRACHYURY and CDX2 mediate BMP-induced differentiation of human and mouse pluripotent stem cells into embryonic and extraembryonic lineages
    Andreia S Bernardo
    The Anne McLaren Laboratory for Regenerative Medicine, University of Cambridge, Cambridge CB2 0SZ, UK
    Cell Stem Cell 9:144-55. 2011
    ..We conclude that BMP induces human and mouse pluripotent stem cells primarily to form mesoderm, rather than trophoblast, acting through BRA and CDX2.
  80. pmc E-cadherin is crucial for embryonic stem cell pluripotency and can replace OCT4 during somatic cell reprogramming
    Torben Redmer
    Max Delbrueck Center, Robert Roessle Strasse 10, 13125 Berlin, Germany Freie Universität Berlin, Department of Biology, Chemistry and Pharmacy, Takustrasse 3, 14195 Berlin, Germany
    EMBO Rep 12:720-6. 2011
    ..Thus, reprogramming requires spatial cues that cross-talk with essential transcription factors. The cell-adhesion molecule E-cadherin has important functions in pluripotency and reprogramming...
  81. ncbi Pluripotent stem cells induced from adult neural stem cells by reprogramming with two factors
    Jeong Beom Kim
    Department of Cell and Developmental Biology, Max Planck Institute for Molecular Biomedicine, Röntgenstrasse 20, 48149 Munster, NRW, Germany
    Nature 454:646-50. 2008
    ..mechanisms of regaining pluripotency and further opens up the possibility of generating patient-specific pluripotent stem cells. Reprogramming of mouse and human somatic cells into pluripotent stem cells, designated as induced ..
  82. pmc Differentiated Parkinson patient-derived induced pluripotent stem cells grow in the adult rodent brain and reduce motor asymmetry in Parkinsonian rats
    Gunnar Hargus
    Udall Parkinson s Disease Research Center of Excellence, McLean Hospital Harvard Medical School, Belmont, MA 02478, USA
    Proc Natl Acad Sci U S A 107:15921-6. 2010
    ....
  83. ncbi Chromatin-Remodeling Components of the BAF Complex Facilitate Reprogramming
    Nishant Singhal
    Department of Cell and Developmental Biology, Max Planck Institute for Molecular Biomedicine, Röntgenstrasse 20, D 48149 Munster, Germany
    Cell 141:943-55. 2010
    ..Thus, somatic cell reprogramming using chromatin-remodeling molecules represents an efficient method of generating reprogrammed cells...
  84. pmc Epigenetic reversion of post-implantation epiblast to pluripotent embryonic stem cells
    Siqin Bao
    Wellcome Trust Cancer Research UK Gurdon Institute, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QN, UK
    Nature 461:1292-5. 2009
    ..Moreover, unlike epiblast and EpiSCs, rESCs contribute to somatic tissues and germ cells in chimaeras. Further studies may reveal how signalling-induced epigenetic reprogramming may promote reacquisition of pluripotency...
  85. pmc Defining molecular cornerstones during fibroblast to iPS cell reprogramming in mouse
    Matthias Stadtfeld
    Massachusetts General Hospital Cancer Center and Center for Regenerative Medicine, 185 Cambridge Street, Boston, MA 02114, USA
    Cell Stem Cell 2:230-40. 2008
    ..Our results suggest that factor-induced reprogramming is a gradual process with defined intermediate cell populations that contain the majority of cells poised to become iPS cells...
  86. ncbi A heterogeneous expression pattern for Nanog in embryonic stem cells
    Amar M Singh
    Department of Pathology, University of Florida College of Medicine, Box 100275, Gainesville, Florida 32610, USA
    Stem Cells 25:2534-42. 2007
    ..Disclosure of potential conflicts of interest is found at the end of this article...
  87. ncbi The transcriptional and signalling networks of pluripotency
    Huck Hui Ng
    Gene Regulation Laboratory, Genome Institute of Singapore, Singapore 138672, Singapore
    Nat Cell Biol 13:490-6. 2011
    ..Here we review the recent advances in studying the transcriptional regulatory networks that define pluripotency, and elaborate on how manipulation of signalling pathways can modulate pluripotent states to varying degrees...
  88. ncbi Extrinsic regulation of pluripotent stem cells
    Martin F Pera
    Center for Regenerative Medicine and Stem Cell Research, Keck School of Medicine, University of Southern California, Los Angeles, California 90033, USA
    Nature 465:713-20. 2010
    ..Understanding the extrinsic control of plasticity will guide efforts to use human pluripotent stem cells in research and therapy.
  89. pmc Cell-surface marker signatures for the isolation of neural stem cells, glia and neurons derived from human pluripotent stem cells
    Shauna H Yuan
    Howard Hughes Medical Institute and Department of Cellular and Molecular Medicine, School of Medicine, University of California San Diego, La Jolla, California, United States of America
    PLoS ONE 6:e17540. 2011
    Neural induction of human pluripotent stem cells often yields heterogeneous cell populations that can hamper quantitative and comparative analyses...
  90. ncbi iPS cells produce viable mice through tetraploid complementation
    Xiao yang Zhao
    State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China
    Nature 461:86-90. 2009
    ..We demonstrate the practicality of using iPS cells as useful tools for the characterization of cellular reprogramming and developmental potency, and confirm that iPS cells can attain true pluripotency that is similar to that of ES cells...
  91. ncbi Oct4-induced pluripotency in adult neural stem cells
    Jeong Beom Kim
    Department of Cell and Developmental Biology, Max Planck Institute for Molecular Biomedicine, Munster, NRW, Germany
    Cell 136:411-9. 2009
    ..that exogenous expression of the germline-specific transcription factor Oct4 is sufficient to generate pluripotent stem cells from adult mouse NSCs...
  92. pmc Generation of pluripotent stem cells from patients with type 1 diabetes
    Rene Maehr
    Department of Stem Cell and Regenerative Biology, Harvard Stem Cell Institute, Harvard University, 7 Divinity Avenue, Cambridge, MA 02138, USA
    Proc Natl Acad Sci U S A 106:15768-73. 2009
    ..These results are a step toward using DiPS cells in T1D disease modeling, as well as for cell replacement therapy...
  93. pmc Global transcription in pluripotent embryonic stem cells
    Sol Efroni
    National Cancer Institute Center for Bioinformatics, National Institutes of Health, Rockville, MD 20852, USA
    Cell Stem Cell 2:437-47. 2008
    ..We propose that global transcription is a hallmark of pluripotent ESCs, contributing to their plasticity, and that lineage specification is driven by reduction of the transcribed portion of the genome...
  94. ncbi Induced pluripotent stem cell generation using a single lentiviral stem cell cassette
    Cesar A Sommer
    Department of Medicine, Boston University School of Medicine, Massachusetts 02118, USA
    Stem Cells 27:543-9. 2009
    ..The use of a single lentiviral vector for reprogramming represents a powerful laboratory tool and a significant step toward the application of iPS technology for clinical purposes...
  95. ncbi Treatment of sickle cell anemia mouse model with iPS cells generated from autologous skin
    Jacob Hanna
    Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA
    Science 318:1920-3. 2007
    ..The problems associated with using retroviruses and oncogenes for reprogramming need to be resolved before iPS cells can be considered for human therapy...
  96. pmc piggyBac transposition reprograms fibroblasts to induced pluripotent stem cells
    Knut Woltjen
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario M5G 1X5, Canada
    Nature 458:766-70. 2009
    ..We anticipate that the unique properties of this virus-independent simplification of iPS cell production will accelerate this field further towards full exploration of the reprogramming process and future cell-based therapies...
  97. ncbi Generation of pluripotent stem cells from adult mouse liver and stomach cells
    Takashi Aoi
    Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
    Science 321:699-702. 2008
    ..These data suggest that iPS cells are generated by direct reprogramming of lineage-committed somatic cells and that retroviral integration into specific sites is not required...
  98. pmc Sequential expression of pluripotency markers during direct reprogramming of mouse somatic cells
    Tobias Brambrink
    Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, 9 Cambridge Center, Cambridge, MA 02142, USA
    Cell Stem Cell 2:151-9. 2008
    ..Importantly, the virally transduced cDNAs needed to be expressed for at least 12 days in order to generate iPS cells. Our results are a step toward understanding some of the molecular events governing epigenetic reprogramming...
  99. pmc Disease-corrected haematopoietic progenitors from Fanconi anaemia induced pluripotent stem cells
    Angel Raya
    Center for Regenerative Medicine in Barcelona, Dr Aiguader 88, 08003 Barcelona, Spain
    Nature 460:53-9. 2009
    ..These data offer proof-of-concept that iPS cell technology can be used for the generation of disease-corrected, patient-specific cells with potential value for cell therapy applications...
  100. pmc Transcriptional signature and memory retention of human-induced pluripotent stem cells
    Maria C N Marchetto
    The Salk Institute for Biological Studies, Laboratory of Genetics, La Jolla, California, USA
    PLoS ONE 4:e7076. 2009
    Genetic reprogramming of somatic cells to a pluripotent state (induced pluripotent stem cells or iPSCs) by over-expression of specific genes has been accomplished using mouse and human cells...
  101. pmc Efficient targeting of expressed and silent genes in human ESCs and iPSCs using zinc-finger nucleases
    Dirk Hockemeyer
    The Whitehead Institute for Biomedical Research, Cambridge, Massachusetts, USA
    Nat Biotechnol 27:851-7. 2009
    Realizing the full potential of human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) requires efficient methods for genetic modification...

Research Grants75

  1. Mice Humanized with Hepatocytes and iPS Cells from Patients with Metabolic Diseas
    Stephen C Strom; Fiscal Year: 2010
    DESCRIPTION (provided by applicant): Specific Challenge Topic 14-DK-101, "Induced Pluripotent stem cells-cellular and humanized mouse" Somatic cells such as fibroblasts from patients with diseases can be used to create cell lines, ..
  2. Patient-Derived Stem Cells for Phosphoproteomic Profiling Neuropsychopathology
    Dieter A Wolf; Fiscal Year: 2011
    ..Recent advances have allowed the conversion of patient-specific human fibroblasts to human induced pluripotent stem cells (hIPSCs), cells which become sufficiently dedifferentiated to a primordial developmental stage that they ..
  3. Model of Timothy Syndrome to Screen Drugs with Induced Pluripotent Stem Cells
    Masayuki Yazawa; Fiscal Year: 2013
    ..to develop human models of TS, I reprogrammed human skin cells from two TS patients to generate induced pluripotent stem cells (iPSCs) and differentiated these cells into CMs...
  4. Model of Timothy Syndrome to Screen Drugs with Induced Pluripotent Stem Cells
    Masayuki Yazawa; Fiscal Year: 2013
    ..to develop human models of TS, I reprogrammed human skin cells from two TS patients to generate induced pluripotent stem cells (iPSCs) and differentiated these cells into CMs...
  5. Improving Epigenetic-based Cell Reprogramming with Proteasome Inhibition
    Jaroslaw Staszkiewicz; Fiscal Year: 2011
    ..raised the possibility that alternative strategies could be developed on an industrial scale to produce pluripotent stem cells without using embryos or genetic manipulations...
  6. Pluripotent stem cell-derived HSCs: improvements and molecular mechanisms
    Patrick Cahan; Fiscal Year: 2013
    ..b>Pluripotent stem cells (PSC), functionally defined by their ability to differentiate into all cells of an organism and their ..
  7. Role of murine induced pluripotent stem cells on the correction of cardiac and sk
    Diego Fraidenraich; Fiscal Year: 2010
    ..These cells were named induced pluripotent stem cells (iPS cells). The iPS cells are similar to the ES cells...
  8. Testing the Therapeutic Potential of iPS Cells for Inherited Skin Diseases
    Jakub Tolar; Fiscal Year: 2013
    ..Therefore, we are proposing to develop stem-cell based therapies for EB using autologous induced pluripotent stem cells (iPSC) derived from skin cells harvested from the same EB patient...
  9. Pluripotent human stem cells as models for normal and diseased trophoblast
    ROBERT MICHAEL ROBERTS; Fiscal Year: 2013
    ..Aim 4 will attempt to re-create EVT from infants born to mothers with PE by generating induced pluripotent stem cells (iPSC) from discarded umbilical cord and converting these pluripotent cells to CT, EVT and other lineages ..
  10. Deriving hepatocytes from disease specific iPS to treat metabolic liver disorders
    Namita Roy-Chowdhury; Fiscal Year: 2013
    ..This research proposal is to test the hypothesis that induced pluripotent stem cells (iPSC) derived from the skin of patients with Crigler-Najjar syndrome type 1 (CN1), who have severe ..
  11. ATM Controls Genomic Stability in Pluripotent Stem Cells
    JASON MARK BECKTA; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Pluripotent stem cells (PSCs) harbor exceptional potential for developing therapeutic advances for many diseases. However, when cultured in vitro, PSCs commonly acquire genetic abnormalities (e.g...
  12. Model of the Human Testis for Reproductive Toxicology
    PAUL JACOB TUREK; Fiscal Year: 2011
    ..hSertCs) and to manipulate primordial germ cells (PGCs), human embryonic stem cells (hESCs), and induced pluripotent stem cells (iPSCs) in vitro to produce haploid cells...
  13. Derivation of cerebral cortical GABAergic interneurons from human iPS cells
    Lorenz P Studer; Fiscal Year: 2010
    ..Induced pluripotent stem cells (iPSCs) are an important potential source of human interneurons that could be used to address both genetic ..
  14. Novel therapies for Fanconi anemia
    DAVID A contact WILLIAMS; Fiscal Year: 2010
    ..One approach that could address this deficiency is the derivation of HSC from induced pluripotent stem cells (iPS) reprogrammed from other somatic tissues...
  15. Pluripotent Stem Cells: Modeling syncytiotrophoblast development and pathogenesis
    Danny J Schust; Fiscal Year: 2013
    ..We will test this hypothesis in vitro using iPSCs derived from the umbilical cords of babies born to mothers with severe preeclampsia and gestational age-matched control iPSCs. ..
  16. INDUCIBLE PLURIPOTENT STEM CELLS AND KIDNEY REGENERATION
    JOSEPH VINCENT BONVENTRE; Fiscal Year: 2010
    ..The purpose of this study is to develop strategies for transforming stem cells into cells of the kidney, with the ultimate goal of regenerating kidney tissue to treat patients with CKD. ..
  17. Studying a Human Stem Cell Disease Using iPS Technology
    Steven E Artandi; Fiscal Year: 2010
    ..Develop molecular signatures for heart, vascular, lung, blood diseases by profiling reprogrammed induced pluripotent stem cells derived from affected individuals of defined genotype...
  18. Genetic correction of human beta-thalassemic induced pluripotent stem cells
    EIRINI PAPAPETROU; Fiscal Year: 2012
    ..gene regulation and on the generation and genetic modification of patient-specific induced pluripotent stem cells (iPSCs)...
  19. Generation of Human iPS Cells via Non-integrating Vectors
    Wen Shu Wu; Fiscal Year: 2010
    ..A particularly exciting method for the generation of patient-specific (or isogenic) pluripotent stem cells is somatic cell reprogramming...
  20. Dysfunction of neurofilaments in disease: an iPS model of Giant Axonal Neuropathy
    BETHANY LESLIE JOHNSON-KERNER; Fiscal Year: 2012
    ..Therefore, I propose to generate a human model of this disease using induced pluripotent stem cells (iPSCs)...
  21. Exploring Human-Induced Pluripotent Stem Cells for Alzheimer's Disease Research
    Kuo Fen Lee; Fiscal Year: 2012
    ..the advent of methodologies to reprogram fully differentiated tissues, such as skin, into human-induced pluripotent stem cells (hiPSCs) has made it possible to study AD in a human physiological context...
  22. Human Pluripotent Stem Cell Differentiation with Defined O2 & Protein Engagement
    ROBERT SAMUEL LANGER; Fiscal Year: 2010
    ..high-throughput microarray platform and culture vessels will be carried out for differentiation of human pluripotent stem cells to cardiomyocytes, which has been shown to benefit from hypoxic culture, as a model system...
  23. There Will Be Blood: Stem Cell Niche Driven Derivation of HSC from ES Cells
    PAUL JOHN SIMMONS; Fiscal Year: 2010
    ..This proposal seeks to develop markedly improved strategies to derive HSC from pluripotent stem cells as a means to overcome this limitation and to further the development of novel cellular therapies for ..
  24. microRNA analysis of neurons generated from patient-specific iPSCs
    Herbert M Lachman; Fiscal Year: 2013
    ..One exciting technological advance we and others are using that could address these limitations is induced pluripotent stem cells (iPSCs)...
  25. Genetic correction of human beta-thalassemic induced pluripotent stem cells
    EIRINI PAPAPETROU; Fiscal Year: 2013
    ..gene regulation and on the generation and genetic modification of patient-specific induced pluripotent stem cells (iPSCs)...
  26. Antibody Mediated Autoimmunity in Neuromyelitis Optica
    HANS CHRISTIAN VON BUEDINGEN; Fiscal Year: 2013
    ..we will also study pathological effects of anti-AQP4 antibodies on astrocytes derived from induced pluripotent stem cells (iPSC) from NMO patients...
  27. Development of Human, Autologous, Pluripotent Very Small Embryonic Like (VSELs) s
    DENIS OVEREND RODGERSON; Fiscal Year: 2013
    ..Our goal is to develop very small embryonic like stem cells (VSEL), which are autologous adult pluripotent stem cells, as a countermeasure to radiological and nuclear threat...
  28. Effect of Smoking on Adult Stem Cell Differentiation
    Herman S Cheung; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): The ability of pluripotent stem cells to generate various replacement cells and tissues highlights their potential application in cell-based regenerative therapies...
  29. Repair of Bone Defects with Human Autologous Pluripotent Very Small Embryonic lik
    Russell S Taichman; Fiscal Year: 2013
    ..Human VSELs are pluripotent stem cells involved in the normal turnover and regeneration of tissues and their circulating levels greatly increase ..
  30. Development of Adult Pluripotent Very Small Embryonic Like (VSEL) Stem Cells to T
    DENIS OVEREND RODGERSON; Fiscal Year: 2013
    ..Studies by NeoStem and its collaborators have shown that within the BM is a resident population of adult, pluripotent stem cells referred to as very small embryonic-like stem cells (VSEL)...
  31. Translating Dosage Compensation to Trisomy
    Jeanne Bentley Lawrence; Fiscal Year: 2010
    ..one X chromosome in mammalian female embryos to "dosage compensate" trisomy 21, primarily in induced pluripotent stem cells which have strong therapeutic potential...
  32. Induced Pluripotent Stem Cells from Swine: application to genetic modification
    ROBERT MICHAEL ROBERTS; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): The goal here is to utilize induced pluripotent stem cells (iPSC) derived from the inner cell mass (ICM) of porcine blastocysts to clone genetically modified pigs by nuclear transfer (NT) procedures...
  33. iPS-derived hepatocytes as a model of familial hypercholesterolemia
    Max A Cayo; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): The generation of induced pluripotent stem cells (iPSC) from adult somatic cells affords a novel approach the study and potential treatment of genetic diseases on a patient-specific level...
  34. Osteoclasts from pluripotent stem cells as vectors to modify bone marrow environm
    Hector L Aguila; Fiscal Year: 2010
    ..these experiments will test the hypothesis that myeloid cells of human origin derived from modified human pluripotent stem cells can localize to the right bone microenvironments, and will provide experimental systems for delivery ..
  35. TRANSPLANTATION OF TESTIS STEM CELLS IN LARGE ANIMALS
    Ina Dobrinski; Fiscal Year: 2013
    ..study the effects of the testicular microenvironment on morphogenesis and germ cell differentiation from pluripotent stem cells using xenografting of isolated testis cells;and 4) to investigate the inductive effect of the ..
  36. Human Stem Cell Model of Niemann Pick Type C
    Lawrence S Goldstein; Fiscal Year: 2010
    ..the first human neuronal model of Niemann Pick type C1 from human embryonic stem cells and human induced pluripotent stem cells. We will use control and NPC1 human neurons to study the kinetics of cholesterol trafficking in NPC1 and ..
  37. Human Blood-Brain Barrier Model for CNS Drug Translation
    Eric V Shusta; Fiscal Year: 2010
    ..solution, we propose creating a blood-brain barrier model based on endothelial cells derived from human pluripotent stem cells (hPSCs)...
  38. Skin-targeted Cell Therapy for Recessive Dystrophic Epidermolysis Bullosa
    Jakub Tolar; Fiscal Year: 2013
    ..homing signal for targeting injured skin in RDEB, using human mesenchymal stromal/stem cells and induced pluripotent stem cells (iPSCs), 2) define the efficacy of systemic RDEB gene therapy by C7 gene augmentation, and 3) use ..
  39. Employing Familial AD Induced Pluripotent Stem Cells to Study Neurodegeneration
    Suman Jayadev; Fiscal Year: 2013
    ..The use of patient derived induced pluripotent stem cells (iPSCs) is an expedient approach to examine the molecular phenotype of specific mutations as well as their ..
  40. Measuring signaling pathway dynamics during tissue growth in hydrogels
    Lonnie D Shea; Fiscal Year: 2013
    ..TFs are key regulators of cell phenotype, as evidenced by their ability to generate pluripotent stem cells from fibroblasts, and they are the downstream targets of signaling pathways, which are the target of many ..
  41. Directed differentiation of embryonic stem cells for urologic applications
    James A Bassuk; Fiscal Year: 2010
    ..of mesenchymal factors necessary for developmental control of bladder tissue formation from inducible pluripotent stem cells, (ii) generating new in vitro models of urothelial cell differentiation and (iii) tissue engineered ..
  42. Human Pluripotent Stem Cell and Progenitor Models of Cardiac and Blood Diseases
    LEONARD IRA ZON; Fiscal Year: 2013
    ..by applicant): Our groups have common interests in the development of improved techniques for generating pluripotent stem cells, directing their differentiation into relevant tissues, and in disease modeling in two major systems of ..
  43. Defining the messenger RNP code in the brain
    EUGENE WEI MING YEO; Fiscal Year: 2013
    ..We will develop a novel resource of human pluripotent stem cells stably expressing tagged RBPs that can be differentiated into human neurons...
  44. Epigenomic Characterization of Alzheimer's Disease Neurons from iPSCs
    Li Huei Tsai; Fiscal Year: 2012
    ..The current proposal will use human neurons derived from patient induced pluripotent stem cells to test the hypothesis that neurons from Alzheimer's disease patients are more susceptible to ..
  45. Rest-Activated Program of Gene Expression in Ischemia
    R Suzanne Zukin; Fiscal Year: 2013
    ..factor that is widely expressed during embryogenesis and plays a strategic role in the transition from pluripotent stem cells to neurons...
  46. Genomic and epigenomic effects of large CNV in neurons from iPSC
    Alexander Eckehart Urban; Fiscal Year: 2012
    ..aim to establish a novel research program and paradigm in our laboratory that combines the use of induced Pluripotent Stem Cells (iPSC) and multi-level, comprehensive and integrated analysis of genomic and epigenomic layers of control ..
  47. CYTOPROTECTIVE EFFECTS OF INFLAMMATION MEDIATED MEMBRANE REPAIR
    DOUGLAS LOWELL MANN; Fiscal Year: 2013
    ..repair and enhanced membrane integrity in isolated rat neonatal cardiac myocytes;and (2) inducible pluripotent stem cells (iPSCs) derived human cardiac myocytes generated from dermal fibroblasts from patients with ..
  48. Monoallelic expression in neurons derived from induced pluripotent stem cells
    Herbert M Lachman; Fiscal Year: 2013
    ....
  49. Oxidative stress and schizophrenia: combination of cell biology and brain imaging
    Akira Sawa; Fiscal Year: 2013
    ..director, has systematically collected tissues and cells (lymphocytes, lymphoblasts, fibroblasts, induced pluripotent stem cells, and olfactory neurons via nasal biopsy) from patients with SZ as well as normal controls...
  50. Human iPS/ES Cell-Based Models for Predictive Neural Toxicity and Teratogenicity
    JAMES ALEXANDER THOMSON; Fiscal Year: 2013
    ..progress has been made in the derivation of many of the basic cellular components of the brain from human pluripotent stem cells (ES and iPS cells), but these advances have yet to be applied to predictive toxicology...
  51. Molecular Basis of Noonan Syndrome and Related Disorder
    Bruce D Gelb; Fiscal Year: 2013
    ..potential efficacy of 50 prioritized compounds in vitro using cardiomyocytes derived from human induced pluripotent stem cells from a RASopathy patient, using regression of hypertrophy as the endpoint...
  52. Establishment of Marmoset iPS-derived Cranial Neural Crest Cells
    Steven Farnsworth; Fiscal Year: 2013
    ..This proposal is innovative because CNC differentiation has not been demonstrated using non-human primate pluripotent stem cells. The proposed research is significant because CNC cells are a key population that defines proper early ..
  53. Regulation and Function of Active DNA Demethylation in Arabidopsis
    Daniel Zilberman; Fiscal Year: 2013
    ..In addition, defective DNA demethylation prevents the in vitro derivation of induced pluripotent stem cells, a technology with the potential to be used in the clinic for the regeneration of cells, tissues and ..
  54. Motor neuron generation from SMA patient-derived induced pluripotent stem cells
    Allison D Ebert; Fiscal Year: 2010
    ....
  55. Mechanisms of dental pulp stem cell differentiation into functional endothelium
    JACQUES EDUARDO NOR; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): The Problem: The dental pulp contains pluripotent stem cells that play a critical role in tooth development and tissue regeneration...
  56. Wnt Signaling in Hematopoietic Stem Cell Specification and Leukemia
    WILSON KENDRICK CLEMENTS; Fiscal Year: 2013
    ..Determining how embryonic stem cells (ESCs) or induced pluripotent stem cells (iPSCs) are directed to become tissue specific stem cells such HSCs is a key goal of regenerative medicine...
  57. Optimizing Normal Collagen Replacement in Osteogenesis Imperfecta
    Sarah L Dallas; Fiscal Year: 2013
    ..research tool with which to explore the utility of transplantation of whole marrow, stem cells or induced pluripotent stem cells (iPS) as potential therapies for repair of abnormal collagen in OI...
  58. Bioprocess for cardiac cell generation from human induced pluripotent stem cells
    EMMANOUHL TZANAKAKIS; Fiscal Year: 2013
    ..Recent advances in the generation of patient-specific human induced pluripotent stem cells (hiPSCs) have sparked hopes that these cells can serve as an inexhaustible source of cellular material for ..
  59. Regulation of Neurogenesis in TSC by mTORC1 and mTORC2
    Kevin C Ess; Fiscal Year: 2013
    ..will use both transgenic mouse models featuring the inactivation of the mouse Tsc1 gene as well as induced pluripotent stem cells (iPSC) we have generated from patients with TSC due to loss of function mutations of the TSC1 or TSC2 ..
  60. Cellular and Genetic Correlates of Increased Head Size in Autism Spectrum Disorde
    Flora M Vaccarino; Fiscal Year: 2010
    Our project capitalizes on recent procedures that allow derivation of pluripotent stem cells from fibroblasts obtainable through a small skin biopsy from living individuals...
  61. TSHR and thyrocyte development
    Reigh Yi Lin; Fiscal Year: 2013
    ..The goal of this project is to combine the advantages of embryonic stem cells and induced pluripotent stem cells with the powerful genetic tools available in mouse models to take a unique approach to thyroid development ..
  62. Cellular and Genetic Correlates of Increased Head Size in Autism Spectrum Disorde
    Flora M Vaccarino; Fiscal Year: 2012
    Our project capitalizes on recent procedures that allow derivation of pluripotent stem cells from fibroblasts obtainable through a small skin biopsy from living individuals...
  63. Pluripotent Adult Spermatogonial Stem Cells: Prospective for Retinal Degeneration
    Nady Golestaneh; Fiscal Year: 2010
    ..stem cells for the first time and under defined culture conditions, we have reprogrammed the SSCs to pluripotent stem cells capable of differentiating into all three germ layers...
  64. PRF 10th Anniversary Workshop on Progeria - "From Bench to Bedside in a Decade"
    Leslie B Gordon; Fiscal Year: 2010
    ..heart disease, lamin biology, and Progeria, as well as experts on cutting edge research such as induced pluripotent stem cells and high throughput assay systems, will contribute to the workshop from both the basic and clinical ..
  65. Mechanisms of Testosterone Effects on Body Composition
    Shalendar Bhasin; Fiscal Year: 2007
    ..validated in vitro models of adult, mesenchymal, stem cell commitment and differentiation, namely, adult, pluripotent stem cells isolated from lipoaspirates, and muscle stem cells isolated from regenerating skeletal muscle of adult ..
  66. Protein-modified Hydrogels for Expansion of Human Embryonic Stem Cells
    TERRY TANDESKI; Fiscal Year: 2009
    The realization of the remarkable potential of human embryonic stem cells or induced pluripotent stem cells to treat human disease cannot occur unless new animal-free methods for the expansion of undifferentiated stem cells are developed...
  67. DIFFERENTIATION OF ENDOCRINE CELL LINEAGES IN GI TRACT
    Andrew Leiter; Fiscal Year: 2001
    ..The Principal Investigator has preliminary data indicating that pluripotent stem cells give rise to PYY progenitors that then differentiate into other endocrine cells...
  68. Antibodies Targeting Novel Surface Antigens on Pluripotent Stem Cell Derivatives
    David Larocca; Fiscal Year: 2009
    ..current limited knowledge of surface markers that define the various cell types that differentiate from pluripotent stem cells. As pluripotent stem cells differentiate there is heterogeneity both spatially, in the cell types formed ..
  69. 2010 Gordon Research Conference on Endothelial Cell Phenotypes in Health and Dise
    Edward Conway; Fiscal Year: 2010
    ..inducible pluripotent stem cells and endothelial progenitor cells, 2...
  70. Multipotent heart cell progenitor lineages in cardiac development and disease
    Kenneth Chien; Fiscal Year: 2009
    ..Islet-1 cardiovascular progenitors from both murine and human embryonic stem (ES) cells and inducible pluripotent stem cells (iPS)...
  71. Use of 3-D Culture and Stretch to Develop Lung from MSCs, ESCs, and iPS
    Daniel J Weiss; Fiscal Year: 2010
    ..approaches utilizing embryonic stem cells (ESCs), adult mesenchymal stem cells (MSCs), or induced pluripotent stem cells (iPS) cultured in three-dimensional culture matrices can regenerate functional lung tissue for replacement ..
  72. Cell-fate determinants of yeast pseudohyphal growth
    Beverly Errede; Fiscal Year: 2005
    ..switch is fundamentally the same as those that occur during the production of distinct lineages from pluripotent stem cells in humans...
  73. Use of Clenbuterol for Enhancement of Stem Cell Therapy
    Richard Zeman; Fiscal Year: 2002
    ..The proposed studies are an outgrowth of previous work in which Beta2-agonists or transplantation of adult pluripotent stem cells into the contusion site enhanced recovery of locomotor function following SCI in rats...
  74. Double-Gated Selection of Ligands that Target Surface Markers of Differentiation
    David Larocca; Fiscal Year: 2009
    ..to pluripotent cells with the same properties as embryonic stem cells have increased the availability of pluripotent stem cells for research and may ultimately provide cells for clinical applications is they can be proven safe...
  75. Molecular Dissection of the Hematopoeic Microenvironment
    Kateri Moore; Fiscal Year: 2005
    ..It has been shown that these pluripotent stem cells reside in discrete niches within a hematopoietic organ...