pluripotent stem cells

Summary

Summary: Cells that can give rise to most types of cells but not all types of cells necessary for fetal development. (Stem Cells: A Primer [Internet]. Bethesda (MD): National Institutes of Health (US); 2000 May [cited 2002 Apr 5]. Available from: http://www.nih.gov/news/stemcell/primer.htm)

Top Publications

  1. ncbi Induced pluripotent stem cell lines derived from human somatic cells
    Junying Yu
    Genome Center of Wisconsin, Madison, WI 53706 1580, USA
    Science 318:1917-20. 2007
  2. ncbi Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors
    Kazutoshi Takahashi
    Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
    Cell 126:663-76. 2006
  3. ncbi Induction of pluripotent stem cells from adult human fibroblasts by defined factors
    Kazutoshi Takahashi
    Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
    Cell 131:861-72. 2007
  4. pmc Genome-wide maps of chromatin state in pluripotent and lineage-committed cells
    Tarjei S Mikkelsen
    Broad Institute of Harvard and MIT, Cambridge, Massachusetts 02142, USA
    Nature 448:553-60. 2007
  5. doi The ground state of embryonic stem cell self-renewal
    Qi Long Ying
    Center for Stem Cell and Regenerative Medicine, Department of Cell and Neurobiology, Keck School of Medicine, University of Southern California, 1501 San Pablo Street, ZNI 529, Los Angeles, California 90033, USA
    Nature 453:519-23. 2008
  6. ncbi Generation of germline-competent induced pluripotent stem cells
    Keisuke Okita
    Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
    Nature 448:313-7. 2007
  7. ncbi Reprogramming of human somatic cells to pluripotency with defined factors
    In Hyun Park
    Division of Pediatric Hematology Oncology, Children s Hospital Boston and Dana Farber Cancer Institute, Boston, Massachusetts 02115, USA
    Nature 451:141-6. 2008
  8. ncbi Nanog safeguards pluripotency and mediates germline development
    Ian Chambers
    MRC Centre Development in Stem Cell Biology, Institute for Stem Cell Research, School of Biological Sciences, University of Edinburgh, King s Buildings, West Mains Road, Edinburgh EH9 3JQ, UK
    Nature 450:1230-4. 2007
  9. pmc Disease-specific induced pluripotent stem cells
    In Hyun Park
    Department of Medicine, Division of Pediatric Hematology Oncology, Children s Hospital Boston, and Dana Farber Cancer Institute, Boston, MA 02115, USA
    Cell 134:877-86. 2008
  10. pmc Genome-scale DNA methylation maps of pluripotent and differentiated cells
    Alexander Meissner
    Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, Massachusetts 02142, USA
    Nature 454:766-70. 2008

Detail Information

Publications355 found, 100 shown here

  1. ncbi Induced pluripotent stem cell lines derived from human somatic cells
    Junying Yu
    Genome Center of Wisconsin, Madison, WI 53706 1580, USA
    Science 318:1917-20. 2007
    ..show that four factors (OCT4, SOX2, NANOG, and LIN28) are sufficient to reprogram human somatic cells to pluripotent stem cells that exhibit the essential characteristics of embryonic stem (ES) cells...
  2. ncbi Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors
    Kazutoshi Takahashi
    Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
    Cell 126:663-76. 2006
    ..Little is known about factors that induce this reprogramming. Here, we demonstrate induction of pluripotent stem cells from mouse embryonic or adult fibroblasts by introducing four factors, Oct3/4, Sox2, c-Myc, and Klf4, ..
  3. ncbi Induction of pluripotent stem cells from adult human fibroblasts by defined factors
    Kazutoshi Takahashi
    Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
    Cell 131:861-72. 2007
    ..Furthermore, these cells could differentiate into cell types of the three germ layers in vitro and in teratomas. These findings demonstrate that iPS cells can be generated from adult human fibroblasts...
  4. pmc Genome-wide maps of chromatin state in pluripotent and lineage-committed cells
    Tarjei S Mikkelsen
    Broad Institute of Harvard and MIT, Cambridge, Massachusetts 02142, USA
    Nature 448:553-60. 2007
    ..This study provides a framework for the application of comprehensive chromatin profiling towards characterization of diverse mammalian cell populations...
  5. doi The ground state of embryonic stem cell self-renewal
    Qi Long Ying
    Center for Stem Cell and Regenerative Medicine, Department of Cell and Neurobiology, Keck School of Medicine, University of Southern California, 1501 San Pablo Street, ZNI 529, Los Angeles, California 90033, USA
    Nature 453:519-23. 2008
    ..This property may account for their latent tumorigenicity. The delineation of minimal requirements for self-renewal now provides a defined platform for the precise description and dissection of the pluripotent state...
  6. ncbi Generation of germline-competent induced pluripotent stem cells
    Keisuke Okita
    Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
    Nature 448:313-7. 2007
    We have previously shown that pluripotent stem cells can be induced from mouse fibroblasts by retroviral introduction of Oct3/4 (also called Pou5f1), Sox2, c-Myc and Klf4, and subsequent selection for Fbx15 (also called Fbxo15) expression...
  7. ncbi Reprogramming of human somatic cells to pluripotency with defined factors
    In Hyun Park
    Division of Pediatric Hematology Oncology, Children s Hospital Boston and Dana Farber Cancer Institute, Boston, Massachusetts 02115, USA
    Nature 451:141-6. 2008
    ..These data demonstrate that defined factors can reprogramme human cells to pluripotency, and establish a method whereby patient-specific cells might be established in culture...
  8. ncbi Nanog safeguards pluripotency and mediates germline development
    Ian Chambers
    MRC Centre Development in Stem Cell Biology, Institute for Stem Cell Research, School of Biological Sciences, University of Edinburgh, King s Buildings, West Mains Road, Edinburgh EH9 3JQ, UK
    Nature 450:1230-4. 2007
    ..We surmise that Nanog stabilizes embryonic stem cells in culture by resisting or reversing alternative gene expression states...
  9. pmc Disease-specific induced pluripotent stem cells
    In Hyun Park
    Department of Medicine, Division of Pediatric Hematology Oncology, Children s Hospital Boston, and Dana Farber Cancer Institute, Boston, MA 02115, USA
    Cell 134:877-86. 2008
    ..Such disease-specific stem cells offer an unprecedented opportunity to recapitulate both normal and pathologic human tissue formation in vitro, thereby enabling disease investigation and drug development...
  10. pmc Genome-scale DNA methylation maps of pluripotent and differentiated cells
    Alexander Meissner
    Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, Massachusetts 02142, USA
    Nature 454:766-70. 2008
    ..More generally, the results establish reduced representation bisulphite sequencing as a powerful technology for epigenetic profiling of cell populations relevant to developmental biology, cancer and regenerative medicine...
  11. pmc Control of the embryonic stem cell state
    Richard A Young
    Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA
    Cell 144:940-54. 2011
    Embryonic stem cells and induced pluripotent stem cells hold great promise for regenerative medicine. These cells can be propagated in culture in an undifferentiated state but can be induced to differentiate into specialized cell types...
  12. doi Generation of mouse induced pluripotent stem cells without viral vectors
    Keisuke Okita
    Center for iPS Cell Research and Application CiRA, Institute for Integrated Cell Material Sciences, Kyoto University, Kyoto 606 8507, Japan
    Science 322:949-53. 2008
    ..The production of virus-free iPS cells, albeit from embryonic fibroblasts, addresses a critical safety concern for potential use of iPS cells in regenerative medicine...
  13. pmc Parkinson's disease patient-derived induced pluripotent stem cells free of viral reprogramming factors
    Frank Soldner
    The Whitehead Institute, Cambridge Center, MA 02142, USA
    Cell 136:964-77. 2009
    Induced pluripotent stem cells (iPSCs) derived from somatic cells of patients represent a powerful tool for biomedical research and may provide a source for replacement therapies...
  14. pmc Induced pluripotent stem cells from a spinal muscular atrophy patient
    Allison D Ebert
    The Waisman Center, The Stem Cell and Regenerative Medicine Center, University of Wisconsin Madison, 1500 Highland Avenue, Madison, Wisconsin 53705, USA
    Nature 457:277-80. 2009
    ..Here we report the generation of induced pluripotent stem cells from skin fibroblast samples taken from a child with spinal muscular atrophy...
  15. ncbi Induced pluripotent stem cells generated from patients with ALS can be differentiated into motor neurons
    John T Dimos
    Harvard Stem Cell Institute, Stowers Medical Institute, Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA 02138, USA
    Science 321:1218-21. 2008
    The generation of pluripotent stem cells from an individual patient would enable the large-scale production of the cell types affected by that patient's disease...
  16. pmc Inhibition of glycogen synthase kinase-3 alleviates Tcf3 repression of the pluripotency network and increases embryonic stem cell resistance to differentiation
    Jason Wray
    Wellcome Trust Centre for Stem Cell Research and Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK
    Nat Cell Biol 13:838-45. 2011
    ..We conclude that Gsk3 inhibition stabilizes the embryonic stem cell state primarily by reducing repressive influence on the core pluripotency network...
  17. pmc Virus-free induction of pluripotency and subsequent excision of reprogramming factors
    Keisuke Kaji
    MRC Centre for Regenerative Medicine, Institute for Stem Cell Research, University of Edinburgh, Edinburgh EH9 3JQ, UK
    Nature 458:771-5. 2009
    ....
  18. pmc Highly efficient neural conversion of human ES and iPS cells by dual inhibition of SMAD signaling
    Stuart M Chambers
    Developmental Biology Program, Sloan Kettering Institute, 1275 York Ave, New York, New York 10065, USA
    Nat Biotechnol 27:275-80. 2009
    ..Noggin/SB431542-based neural induction should facilitate the use of hES and hiPS cells in regenerative medicine and disease modeling and obviate the need for protocols based on stromal feeders or embryoid bodies...
  19. pmc Modelling pathogenesis and treatment of familial dysautonomia using patient-specific iPSCs
    Gabsang Lee
    Developmental Biology Program, Sloan Kettering Institute, 1275 York Ave, USA
    Nature 461:402-6. 2009
    The isolation of human induced pluripotent stem cells (iPSCs) offers a new strategy for modelling human disease. Recent studies have reported the derivation and differentiation of disease-specific human iPSCs...
  20. pmc Dynamic changes in the copy number of pluripotency and cell proliferation genes in human ESCs and iPSCs during reprogramming and time in culture
    Louise C Laurent
    Department of Reproductive Medicine, University of California, San Diego, La Jolla, CA 92093, USA
    Cell Stem Cell 8:106-18. 2011
    Genomic stability is critical for the clinical use of human embryonic and induced pluripotent stem cells. We performed high-resolution SNP (single-nucleotide polymorphism) analysis on 186 pluripotent and 119 nonpluripotent samples...
  21. pmc Dissecting direct reprogramming through integrative genomic analysis
    Tarjei S Mikkelsen
    Broad Institute of MIT and Harvard, 7 Cambridge Center, Cambridge, Massachusetts 02142, USA
    Nature 454:49-55. 2008
    ..We demonstrate that RNA inhibition of transcription factors can facilitate reprogramming, and that treatment with DNA methyltransferase inhibitors can improve the overall efficiency of the reprogramming process...
  22. pmc Induced pluripotent stem cells and embryonic stem cells are distinguished by gene expression signatures
    Mark H Chin
    Department of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA
    Cell Stem Cell 5:111-23. 2009
    Induced pluripotent stem cells (iPSCs) outwardly appear to be indistinguishable from embryonic stem cells (ESCs)...
  23. ncbi New cell lines from mouse epiblast share defining features with human embryonic stem cells
    Paul J Tesar
    Laboratory of Molecular Biology, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland 20892, USA
    Nature 448:196-9. 2007
    ..These results show that epiblast cells can be maintained as stable cell lines and interrogated to understand how pluripotent cells generate distinct fates during early development...
  24. ncbi Derivation of pluripotent epiblast stem cells from mammalian embryos
    I Gabrielle M Brons
    Department of Surgery and Cambridge Institute for Medical Research, Addenbrooke s Hospital, University of Cambridge, Cambridge CB2 0XY, UK
    Nature 448:191-5. 2007
    ..Here we show that pluripotent stem cells can be derived from the late epiblast layer of post-implantation mouse and rat embryos using chemically ..
  25. pmc Regulated fluctuations in nanog expression mediate cell fate decisions in embryonic stem cells
    Tibor Kalmar
    Department of Genetics, University of Cambridge, Cambridge, United Kingdom
    PLoS Biol 7:e1000149. 2009
    ....
  26. ncbi In vitro reprogramming of fibroblasts into a pluripotent ES-cell-like state
    Marius Wernig
    Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02142, USA
    Nature 448:318-24. 2007
    ..Our results show that the biological potency and epigenetic state of in-vitro-reprogrammed induced pluripotent stem cells are indistinguishable from those of ES cells.
  27. ncbi Polycomb complexes repress developmental regulators in murine embryonic stem cells
    Laurie A Boyer
    Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, Massachusetts 02142, USA
    Nature 441:349-53. 2006
    ..Our results indicate that dynamic repression of developmental pathways by Polycomb complexes may be required for maintaining ES cell pluripotency and plasticity during embryonic development...
  28. pmc Modelling schizophrenia using human induced pluripotent stem cells
    Kristen J Brennand
    Salk Institute for Biological Studies, Laboratory of Genetics, 10010 North Torrey Pines Road, La Jolla California 92037, USA
    Nature 473:221-5. 2011
    ..and molecular defects of SCZD, we directly reprogrammed fibroblasts from SCZD patients into human induced pluripotent stem cells (hiPSCs) and subsequently differentiated these disorder-specific hiPSCs into neurons (Supplementary Fig...
  29. pmc Pluripotency and cellular reprogramming: facts, hypotheses, unresolved issues
    Jacob H Hanna
    The Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Cambridge, MA 02142, USA
    Cell 143:508-25. 2010
    Direct reprogramming of somatic cells to induced pluripotent stem cells by ectopic expression of defined transcription factors has raised fundamental questions regarding the epigenetic stability of the differentiated cell state...
  30. pmc Induced pluripotent stem cells generated without viral integration
    Matthias Stadtfeld
    Massachusetts General Hospital Cancer Center and Center for Regenerative Medicine, 185 Cambridge Street, Boston, MA 02114, USA
    Science 322:945-9. 2008
    b>Pluripotent stem cells have been generated from mouse and human somatic cells by viral expression of the transcription factors Oct4, Sox2, Klf4, and c-Myc...
  31. ncbi Lineage-specific polycomb targets and de novo DNA methylation define restriction and potential of neuronal progenitors
    Fabio Mohn
    Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, 4058 Basel, Switzerland
    Mol Cell 30:755-66. 2008
    ..These data suggest a model how de novo DNA methylation and dynamic switches in Polycomb targets restrict pluripotency and define the developmental potential of progenitor cells...
  32. pmc Generation of isogenic pluripotent stem cells differing exclusively at two early onset Parkinson point mutations
    Frank Soldner
    The Whitehead Institute, 9 Cambridge Center, Cambridge, MA 02142, USA
    Cell 146:318-31. 2011
    Patient-specific induced pluripotent stem cells (iPSCs) derived from somatic cells provide a unique tool for the study of human disease, as well as a promising source for cell replacement therapies...
  33. ncbi Adult mice generated from induced pluripotent stem cells
    Michael J Boland
    Department of Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Nature 461:91-4. 2009
    ....
  34. pmc Aberrant silencing of imprinted genes on chromosome 12qF1 in mouse induced pluripotent stem cells
    Matthias Stadtfeld
    Howard Hughes Medical Institute at Massachusetts General Hospital, Center for Regenerative Medicine Harvard Stem Cell Institute, 185 Cambridge Street, Boston, Massachusetts 02114, USA
    Nature 465:175-81. 2010
    Induced pluripotent stem cells (iPSCs) have been generated by enforced expression of defined sets of transcription factors in somatic cells...
  35. pmc Direct cell reprogramming is a stochastic process amenable to acceleration
    Jacob Hanna
    The Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, USA
    Nature 462:595-601. 2009
    ....
  36. pmc Dynamic equilibrium and heterogeneity of mouse pluripotent stem cells with distinct functional and epigenetic states
    Katsuhiko Hayashi
    Wellcome Trust Cancer Research UK Gurdon Institute, The Henry Wellcome Building of Cancer and Developmental Biology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK
    Cell Stem Cell 3:391-401. 2008
    ....
  37. pmc Human embryonic stem cells with biological and epigenetic characteristics similar to those of mouse ESCs
    Jacob Hanna
    The Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA
    Proc Natl Acad Sci U S A 107:9222-7. 2010
    ..The generation of validated "naïve" human ESCs will allow the molecular dissection of a previously undefined pluripotent state in humans and may open up new opportunities for patient-specific, disease-relevant research...
  38. doi Stage-specific optimization of activin/nodal and BMP signaling promotes cardiac differentiation of mouse and human pluripotent stem cell lines
    Steven J Kattman
    McEwen Center for Regenerative Medicine, University Health Network, Toronto, Ontario, Canada
    Cell Stem Cell 8:228-40. 2011
    Efficient differentiation of embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) to a variety of lineages requires step-wise approaches replicating the key commitment stages found during embryonic development...
  39. ncbi The homeoprotein Nanog is required for maintenance of pluripotency in mouse epiblast and ES cells
    Kaoru Mitsui
    Laboratory of Animal Molecular Technology, Research and Education Center for Genetic Information, Nara Institute of Science and Technology, Nara 630 0192, Japan
    Cell 113:631-42. 2003
    ..nanog-deficient ES cells lost pluripotency and differentiated into extraembryonic endoderm lineage. These data demonstrate that Nanog is a critical factor underlying pluripotency in both ICM and ES cells...
  40. pmc Human induced pluripotent stem cells free of vector and transgene sequences
    Junying Yu
    Morgridge Institute for Research, Madison, WI 53707 7365, USA
    Science 324:797-801. 2009
    ..These results demonstrate that reprogramming human somatic cells does not require genomic integration or the continued presence of exogenous reprogramming factors and removes one obstacle to the clinical application of human iPS cells...
  41. pmc Incomplete DNA methylation underlies a transcriptional memory of somatic cells in human iPS cells
    Yuki Ohi
    Department of Ob Gyn and Center for Reproductive Sciences, University of California San Francisco, 513 Parnassus Avenue, San Francisco, California 94143, USA
    Nat Cell Biol 13:541-9. 2011
    ..Knockdown of the incompletely reprogrammed gene C9orf64 (chromosome 9 open reading frame 64) reduces the efficiency of human iPS cell generation, indicating that somatic memory genes may be functionally relevant during reprogramming...
  42. ncbi Generation of induced pluripotent stem cells using recombinant proteins
    Hongyan Zhou
    Department of Chemistry, The Scripps Research Institute, La Jolla, CA 92037, USA
    Cell Stem Cell 4:381-4. 2009
  43. ncbi A parallel circuit of LIF signalling pathways maintains pluripotency of mouse ES cells
    Hitoshi Niwa
    Laboratory for Pluripotent Cell Studies, RIKEN Center for Developmental Biology, 2 2 3 Minatojima minamimachi, Chuo Ku, Kobe 6500047, Japan
    Nature 460:118-22. 2009
    ....
  44. doi Naive and primed pluripotent states
    Jennifer Nichols
    Wellcome Trust Centre for Stem Cell Research, Department of Physiology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK
    Cell Stem Cell 4:487-92. 2009
    ..We propose that two phases of pluripotency can be defined: naive and primed. This distinction extends to pluripotent stem cells derived from embryos or by molecular reprogramming ex vivo.
  45. ncbi Generation of induced pluripotent stem cells without Myc from mouse and human fibroblasts
    Masato Nakagawa
    Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
    Nat Biotechnol 26:101-6. 2008
    Direct reprogramming of somatic cells provides an opportunity to generate patient- or disease-specific pluripotent stem cells. Such induced pluripotent stem (iPS) cells were generated from mouse fibroblasts by retroviral transduction of ..
  46. doi Direct reprogramming of human neural stem cells by OCT4
    Jeong Beom Kim
    Max Planck Institute for Molecular Biomedicine, Department of Cell and Developmental Biology, Röntgenstrasse 20, 48149 Munster, NRW, Germany
    Nature 461:649-3. 2009
    ..One-factor iPS cell generation will advance the field further towards understanding reprogramming and generating patient-specific pluripotent stem cells.
  47. pmc Functional cardiomyocytes derived from human induced pluripotent stem cells
    Jianhua Zhang
    Department of Medicine, University of Wisconsin, WiCell Research Institute, Madison, WI 53792 3248, USA
    Circ Res 104:e30-41. 2009
    ..We conclude that human iPS cells can differentiate into functional cardiomyocytes, and thus iPS cells are a viable option as an autologous cell source for cardiac repair and a powerful tool for cardiovascular research...
  48. pmc The transcriptional foundation of pluripotency
    Ian Chambers
    MRC Centre for Regenerative Medicine, Institute for Stem Cell Research, School of Biological Sciences, University of Edinburgh, King s Buildings, Edinburgh EH9 3JQ, UK
    Development 136:2311-22. 2009
    ..Here we discuss how these observations advance our understanding of the transcription factor network that controls pluripotent identity and highlight unresolved issues that arise from these studies...
  49. pmc Klf4 reverts developmentally programmed restriction of ground state pluripotency
    Ge Guo
    Wellcome Trust Centre for Stem Cell Research, University of Cambridge, Cambridge CB2 1QR, UK
    Development 136:1063-9. 2009
    ..However, because a single transgene is the minimum requirement to attain the ground state, EpiSCs offer an attractive opportunity for screening for unknown components of the reprogramming process...
  50. pmc Neural differentiation of human induced pluripotent stem cells follows developmental principles but with variable potency
    Bao Yang Hu
    Department of Anatomy, School of Medicine and Public Health, University of Wisconsin, Waisman Center, Madison, WI 53705, USA
    Proc Natl Acad Sci U S A 107:4335-40. 2010
    For the promise of human induced pluripotent stem cells (iPSCs) to be realized, it is necessary to ask if and how efficiently they may be differentiated to functional cells of various lineages...
  51. pmc E-cadherin is crucial for embryonic stem cell pluripotency and can replace OCT4 during somatic cell reprogramming
    Torben Redmer
    Max Delbrueck Center, Robert Roessle Strasse 10, 13125 Berlin, Germany Freie Universität Berlin, Department of Biology, Chemistry and Pharmacy, Takustrasse 3, 14195 Berlin, Germany
    EMBO Rep 12:720-6. 2011
    ..Thus, reprogramming requires spatial cues that cross-talk with essential transcription factors. The cell-adhesion molecule E-cadherin has important functions in pluripotency and reprogramming...
  52. pmc BRACHYURY and CDX2 mediate BMP-induced differentiation of human and mouse pluripotent stem cells into embryonic and extraembryonic lineages
    Andreia S Bernardo
    The Anne McLaren Laboratory for Regenerative Medicine, University of Cambridge, Cambridge CB2 0SZ, UK
    Cell Stem Cell 9:144-55. 2011
    ..We conclude that BMP induces human and mouse pluripotent stem cells primarily to form mesoderm, rather than trophoblast, acting through BRA and CDX2.
  53. ncbi Pluripotent stem cells induced from adult neural stem cells by reprogramming with two factors
    Jeong Beom Kim
    Department of Cell and Developmental Biology, Max Planck Institute for Molecular Biomedicine, Röntgenstrasse 20, 48149 Munster, NRW, Germany
    Nature 454:646-50. 2008
    ..mechanisms of regaining pluripotency and further opens up the possibility of generating patient-specific pluripotent stem cells. Reprogramming of mouse and human somatic cells into pluripotent stem cells, designated as induced ..
  54. pmc Promotion of reprogramming to ground state pluripotency by signal inhibition
    Jose Silva
    Wellcome Trust Centre for Stem Cell Research, University of Cambridge, Cambridge, United Kingdom
    PLoS Biol 6:e253. 2008
    ..We therefore applied molecularly defined conditions for the derivation and propagation of authentic pluripotent stem cells from embryos...
  55. pmc Differentiated Parkinson patient-derived induced pluripotent stem cells grow in the adult rodent brain and reduce motor asymmetry in Parkinsonian rats
    Gunnar Hargus
    Udall Parkinson s Disease Research Center of Excellence, McLean Hospital Harvard Medical School, Belmont, MA 02478, USA
    Proc Natl Acad Sci U S A 107:15921-6. 2010
    ....
  56. ncbi Chromatin-Remodeling Components of the BAF Complex Facilitate Reprogramming
    Nishant Singhal
    Department of Cell and Developmental Biology, Max Planck Institute for Molecular Biomedicine, Röntgenstrasse 20, D 48149 Munster, Germany
    Cell 141:943-55. 2010
    ..Thus, somatic cell reprogramming using chromatin-remodeling molecules represents an efficient method of generating reprogrammed cells...
  57. pmc Epigenetic reversion of post-implantation epiblast to pluripotent embryonic stem cells
    Siqin Bao
    Wellcome Trust Cancer Research UK Gurdon Institute, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QN, UK
    Nature 461:1292-5. 2009
    ..Moreover, unlike epiblast and EpiSCs, rESCs contribute to somatic tissues and germ cells in chimaeras. Further studies may reveal how signalling-induced epigenetic reprogramming may promote reacquisition of pluripotency...
  58. ncbi The ground state of pluripotency
    Jason Wray
    Wellcome Trust Centre for Stem Cell Research, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK
    Biochem Soc Trans 38:1027-32. 2010
    ..The stability of this state is reflected in the homogeneity of ES cell populations cultured in the presence of small-molecule inhibitors of MEK (mitogen-activated protein kinase/ERK kinase) and GSK3...
  59. pmc Defining molecular cornerstones during fibroblast to iPS cell reprogramming in mouse
    Matthias Stadtfeld
    Massachusetts General Hospital Cancer Center and Center for Regenerative Medicine, 185 Cambridge Street, Boston, MA 02114, USA
    Cell Stem Cell 2:230-40. 2008
    ..Our results suggest that factor-induced reprogramming is a gradual process with defined intermediate cell populations that contain the majority of cells poised to become iPS cells...
  60. ncbi A heterogeneous expression pattern for Nanog in embryonic stem cells
    Amar M Singh
    Department of Pathology, University of Florida College of Medicine, Box 100275, Gainesville, Florida 32610, USA
    Stem Cells 25:2534-42. 2007
    ..Disclosure of potential conflicts of interest is found at the end of this article...
  61. ncbi Maintenance of pluripotency in human and mouse embryonic stem cells through activation of Wnt signaling by a pharmacological GSK-3-specific inhibitor
    Noboru Sato
    Laboratory of Molecular Vertebrate Embryology, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
    Nat Med 10:55-63. 2004
    ..These results suggest that the use of GSK-3-specific inhibitors such as BIO may have practical applications in regenerative medicine...
  62. pmc Cell-surface marker signatures for the isolation of neural stem cells, glia and neurons derived from human pluripotent stem cells
    Shauna H Yuan
    Howard Hughes Medical Institute and Department of Cellular and Molecular Medicine, School of Medicine, University of California San Diego, La Jolla, California, United States of America
    PLoS ONE 6:e17540. 2011
    Neural induction of human pluripotent stem cells often yields heterogeneous cell populations that can hamper quantitative and comparative analyses...
  63. doi The transcriptional and signalling networks of pluripotency
    Huck Hui Ng
    Gene Regulation Laboratory, Genome Institute of Singapore, Singapore 138672, Singapore
    Nat Cell Biol 13:490-6. 2011
    ..Here we review the recent advances in studying the transcriptional regulatory networks that define pluripotency, and elaborate on how manipulation of signalling pathways can modulate pluripotent states to varying degrees...
  64. ncbi Extrinsic regulation of pluripotent stem cells
    Martin F Pera
    Center for Regenerative Medicine and Stem Cell Research, Keck School of Medicine, University of Southern California, Los Angeles, California 90033, USA
    Nature 465:713-20. 2010
    ..Understanding the extrinsic control of plasticity will guide efforts to use human pluripotent stem cells in research and therapy.
  65. ncbi iPS cells produce viable mice through tetraploid complementation
    Xiao yang Zhao
    State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China
    Nature 461:86-90. 2009
    ..We demonstrate the practicality of using iPS cells as useful tools for the characterization of cellular reprogramming and developmental potency, and confirm that iPS cells can attain true pluripotency that is similar to that of ES cells...
  66. doi Oct4-induced pluripotency in adult neural stem cells
    Jeong Beom Kim
    Department of Cell and Developmental Biology, Max Planck Institute for Molecular Biomedicine, Munster, NRW, Germany
    Cell 136:411-9. 2009
    ..that exogenous expression of the germline-specific transcription factor Oct4 is sufficient to generate pluripotent stem cells from adult mouse NSCs...
  67. pmc Global transcription in pluripotent embryonic stem cells
    Sol Efroni
    National Cancer Institute Center for Bioinformatics, National Institutes of Health, Rockville, MD 20852, USA
    Cell Stem Cell 2:437-47. 2008
    ..We propose that global transcription is a hallmark of pluripotent ESCs, contributing to their plasticity, and that lineage specification is driven by reduction of the transcribed portion of the genome...
  68. pmc Generation of pluripotent stem cells from patients with type 1 diabetes
    Rene Maehr
    Department of Stem Cell and Regenerative Biology, Harvard Stem Cell Institute, Harvard University, 7 Divinity Avenue, Cambridge, MA 02138, USA
    Proc Natl Acad Sci U S A 106:15768-73. 2009
    ..These results are a step toward using DiPS cells in T1D disease modeling, as well as for cell replacement therapy...
  69. pmc Suppression of Erk signalling promotes ground state pluripotency in the mouse embryo
    Jennifer Nichols
    Wellcome Trust Centre for Stem Cell Research, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK
    Development 136:3215-22. 2009
    ..We propose that ES cells are not a tissue culture creation, but are essentially identical to pre-implantation epiblast cells...
  70. pmc Rapid and efficient reprogramming of somatic cells to induced pluripotent stem cells by retinoic acid receptor gamma and liver receptor homolog 1
    Wei Wang
    Wellcome Trust Sanger Institute, Hinxton CB10 1HH, United Kingdom
    Proc Natl Acad Sci U S A 108:18283-8. 2011
    Somatic cells can be reprogrammed to induced pluripotent stem cells (iPSCs) by expressing four transcription factors: Oct4, Sox2, Klf4, and c-Myc...
  71. doi Induced pluripotent stem cell generation using a single lentiviral stem cell cassette
    Cesar A Sommer
    Department of Medicine, Boston University School of Medicine, Massachusetts 02118, USA
    Stem Cells 27:543-9. 2009
    ..The use of a single lentiviral vector for reprogramming represents a powerful laboratory tool and a significant step toward the application of iPS technology for clinical purposes...
  72. ncbi Treatment of sickle cell anemia mouse model with iPS cells generated from autologous skin
    Jacob Hanna
    Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA
    Science 318:1920-3. 2007
    ..The problems associated with using retroviruses and oncogenes for reprogramming need to be resolved before iPS cells can be considered for human therapy...
  73. pmc piggyBac transposition reprograms fibroblasts to induced pluripotent stem cells
    Knut Woltjen
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario M5G 1X5, Canada
    Nature 458:766-70. 2009
    ..We anticipate that the unique properties of this virus-independent simplification of iPS cell production will accelerate this field further towards full exploration of the reprogramming process and future cell-based therapies...
  74. ncbi Generation of pluripotent stem cells from adult mouse liver and stomach cells
    Takashi Aoi
    Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
    Science 321:699-702. 2008
    ..These data suggest that iPS cells are generated by direct reprogramming of lineage-committed somatic cells and that retroviral integration into specific sites is not required...
  75. pmc Transcriptional signature and memory retention of human-induced pluripotent stem cells
    Maria C N Marchetto
    The Salk Institute for Biological Studies, Laboratory of Genetics, La Jolla, California, USA
    PLoS ONE 4:e7076. 2009
    Genetic reprogramming of somatic cells to a pluripotent state (induced pluripotent stem cells or iPSCs) by over-expression of specific genes has been accomplished using mouse and human cells...
  76. pmc Disease-corrected haematopoietic progenitors from Fanconi anaemia induced pluripotent stem cells
    Angel Raya
    Center for Regenerative Medicine in Barcelona, Dr Aiguader 88, 08003 Barcelona, Spain
    Nature 460:53-9. 2009
    ..These data offer proof-of-concept that iPS cell technology can be used for the generation of disease-corrected, patient-specific cells with potential value for cell therapy applications...
  77. pmc Sequential expression of pluripotency markers during direct reprogramming of mouse somatic cells
    Tobias Brambrink
    Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, 9 Cambridge Center, Cambridge, MA 02142, USA
    Cell Stem Cell 2:151-9. 2008
    ..Importantly, the virally transduced cDNAs needed to be expressed for at least 12 days in order to generate iPS cells. Our results are a step toward understanding some of the molecular events governing epigenetic reprogramming...
  78. pmc Efficient targeting of expressed and silent genes in human ESCs and iPSCs using zinc-finger nucleases
    Dirk Hockemeyer
    The Whitehead Institute for Biomedical Research, Cambridge, Massachusetts, USA
    Nat Biotechnol 27:851-7. 2009
    Realizing the full potential of human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) requires efficient methods for genetic modification...
  79. doi Generation of pluripotent stem cells from adult human testis
    Sabine Conrad
    Institute of Anatomy, Department of Experimental Embryology, Tubingen, Germany
    Nature 456:344-9. 2008
    ....
  80. ncbi Induction of pluripotent stem cells from fibroblast cultures
    Kazutoshi Takahashi
    Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara machi, Shogoin, Sakyo ku, Kyoto 606 8507, Japan
    Nat Protoc 2:3081-9. 2007
    ..One way to circumvent these issues is to generate pluripotent stem cells directly from somatic cells...
  81. pmc Direct reprogramming of terminally differentiated mature B lymphocytes to pluripotency
    Jacob Hanna
    The Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA
    Cell 133:250-64. 2008
    ..Our study provides definite proof for the direct nuclear reprogramming of terminally differentiated adult cells to pluripotency...
  82. pmc Myc represses primitive endoderm differentiation in pluripotent stem cells
    Keriayn N Smith
    Department of Biochemistry and Molecular Biology, Paul D Coverdell Center for Biomedical and Health Sciences, The University of Georgia, 500 D W Brooks Drive, Athens, GA 30602, USA
    Cell Stem Cell 7:343-54. 2010
    The generation of induced pluripotent stem cells (iPSCs) provides a novel method to facilitate investigations into the mechanisms that control stem cell pluripotency and self-renewal...
  83. doi Lab-specific gene expression signatures in pluripotent stem cells
    Aaron M Newman
    Biomolecular Science and Engineering Program, University of California, Santa Barbara, CA 93106, USA
    Cell Stem Cell 7:258-62. 2010
    b>Pluripotent stem cells derived from both embryonic and reprogrammed somatic cells have significant potential for human regenerative medicine...
  84. doi Variation in the safety of induced pluripotent stem cell lines
    Kyoko Miura
    Center for iPS Cell Research and Application CiRA, Kyoto University, Kyoto, Japan
    Nat Biotechnol 27:743-5. 2009
    ..In contrast, SNS from iPS cells derived from different adult tissues varied substantially in their teratoma-forming propensity, which correlated with the persistence of undifferentiated cells...
  85. pmc Distinct epigenomic landscapes of pluripotent and lineage-committed human cells
    R David Hawkins
    Ludwig Institute for Cancer Research, La Jolla, CA 92093, USA
    Cell Stem Cell 6:479-91. 2010
    ..Our results provide new insights into epigenetic mechanisms underlying properties of pluripotency and cell fate commitment...
  86. pmc Regulatory networks define phenotypic classes of human stem cell lines
    Franz Josef Muller
    Center for Regenerative Medicine, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Nature 455:401-5. 2008
    ..Our results offer a new strategy for classifying stem cells and support the idea that pluripotency and self-renewal are under tight control by specific molecular networks...
  87. ncbi Cardiomyocyte differentiation of human induced pluripotent stem cells
    Limor Zwi
    Sohnis Family Research Laboratory for Cardiac Electrophysiology and Regenerative Medicine, The Rappaport Family Institute for Research in the Medical Sciences, Technion Israel Institute of Technology, Haifa, Israel
    Circulation 120:1513-23. 2009
    ..In the present study, we aimed to characterize the cardiomyocyte differentiation potential of hiPS cells and to study the molecular, structural, and functional properties of the generated hiPS-derived cardiomyocytes...
  88. pmc Repair of acute myocardial infarction by human stemness factors induced pluripotent stem cells
    Timothy J Nelson
    Mayo Clinic, 200 First St SW, Rochester, MN 55905, USA
    Circulation 120:408-16. 2009
    Nuclear reprogramming provides an emerging strategy to produce embryo-independent pluripotent stem cells from somatic tissue...
  89. ncbi Pluripotency of spermatogonial stem cells from adult mouse testis
    Kaomei Guan
    Department of Cardiology and Pneumology, Heart Center, Georg August University of Gottingen, Robert Koch Str 40, 37075 Gottingen, Germany
    Nature 440:1199-203. 2006
    ..Furthermore, these cells may provide new opportunities to study genetic diseases in various cell lineages...
  90. pmc A functionally characterized test set of human induced pluripotent stem cells
    Gabriella L Boulting
    The Howard Hughes Medical Institute, Cambridge, Massachusetts, USA
    Nat Biotechnol 29:279-86. 2011
    Human induced pluripotent stem cells (iPSCs) present exciting opportunities for studying development and for in vitro disease modeling. However, reported variability in the behavior of iPSCs has called their utility into question...
  91. ncbi Functional expression cloning of Nanog, a pluripotency sustaining factor in embryonic stem cells
    Ian Chambers
    Institute for Stem Cell Research, University of Edinburgh, King s Buildings, West Mains Road, Edinburgh EH9 3JQ, Scotland
    Cell 113:643-55. 2003
    ..These findings establish a central role for Nanog in the transcription factor hierarchy that defines ES cell identity...
  92. pmc Engineering of human pluripotent stem cells by AAV-mediated gene targeting
    Iram F Khan
    Department of Medicine, University of Washington, Seattle, Washington 98195, USA
    Mol Ther 18:1192-9. 2010
    Precise genetic manipulation of human pluripotent stem cells will be required to realize their scientific and therapeutic potential...
  93. pmc Chd1 regulates open chromatin and pluripotency of embryonic stem cells
    Alexandre Gaspar-Maia
    Department of Ob Gyn and Pathology, Center for Reproductive Sciences and Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, San Francisco, 513 Parnassus Avenue, San Francisco, California 94143 0525, USA
    Nature 460:863-8. 2009
    ..Our results indicate that Chd1 is essential for open chromatin and pluripotency of embryonic stem cells, and for somatic cell reprogramming to the pluripotent state...
  94. doi Stepwise differentiation of pluripotent stem cells into retinal cells
    Fumitaka Osakada
    Laboratory for Retinal Regeneration, Center for Developmental Biology, RIKEN, Kobe, Japan
    Nat Protoc 4:811-24. 2009
    ..It will also improve our understanding of the development of the central nervous system, especially the eye...
  95. pmc Metastable pluripotent states in NOD-mouse-derived ESCs
    Jacob Hanna
    The Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA
    Cell Stem Cell 4:513-24. 2009
    ..Our findings suggest that stem cells from different genetic backgrounds can assume distinct states of pluripotency in vitro, the stability of which is regulated by endogenous genetic determinants and can be modified by exogenous factors...
  96. pmc A high-efficiency system for the generation and study of human induced pluripotent stem cells
    Nimet Maherali
    Department of Stem Cell and Regenerative Biology, Harvard Stem Cell Institute, Massachusetts General Hospital Center for Regenerative Medicine, Massachusetts General Hospital Cancer Center, Boston, MA 02114, USA
    Cell Stem Cell 3:340-5. 2008
    ..lentiviral system to convert primary human fibroblasts and keratinocytes into human induced pluripotent stem cells (hiPSCs)...
  97. doi Strategies and new developments in the generation of patient-specific pluripotent stem cells
    Shinya Yamanaka
    Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
    Cell Stem Cell 1:39-49. 2007
    Generating pluripotent stem cells directly from cells obtained from patients is one of the ultimate goals in regenerative medicine...
  98. pmc Neurons derived from reprogrammed fibroblasts functionally integrate into the fetal brain and improve symptoms of rats with Parkinson's disease
    Marius Wernig
    The Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA
    Proc Natl Acad Sci U S A 105:5856-61. 2008
    ..Our results demonstrate the therapeutic potential of directly reprogrammed fibroblasts for neuronal cell replacement in the animal model...
  99. doi Highly efficient differentiation of human ES cells and iPS cells into mature pancreatic insulin-producing cells
    Donghui Zhang
    Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education, College of Life Sciences, Peking University, Beijing 100871, China
    Cell Res 19:429-38. 2009
    Human pluripotent stem cells represent a potentially unlimited source of functional pancreatic endocrine lineage cells...
  100. pmc Genome-wide analysis reveals Sall4 to be a major regulator of pluripotency in murine-embryonic stem cells
    Jianchang Yang
    Division of Laboratory Medicine, Nevada Cancer Institute, One Breakthrough Way, Las Vegas, NV 89135, USA
    Proc Natl Acad Sci U S A 105:19756-61. 2008
    ..This suggests that Sall4 plays a diverse role in regulating stem cell pluripotency during early embryonic development through integration of transcriptional and epigenetic controls...
  101. ncbi Nuclear reprogramming and pluripotency
    Konrad Hochedlinger
    Department of Biology, Massachusetts Institute of Technology, 9 Cambridge Center, Cambridge, Massachusetts 02142, USA
    Nature 441:1061-7. 2006
    ..The future challenge will be to study alternatives to nuclear transfer in order to recapitulate reprogramming in a Petri dish without the use of oocytes...

Research Grants111 found, 100 shown here

  1. Protein-induced human iPS cells for personalized cell therapy of PD
    Kwang Soo Kim; Fiscal Year: 2010
    ..In 2006, Shinya Yamanaka and his colleagues published their groundbreaking work showing that pluripotent stem cells, so called "induced pluripotent stem cells (iPSCs)", can be generated from somatic cells by retroviral ..
  2. Pluripotent human stem cells as models for normal and diseased trophoblast
    ROBERT MICHAEL ROBERTS; Fiscal Year: 2011
    ..Aim 4 will attempt to re-create EVT from infants born to mothers with PE by generating induced pluripotent stem cells (iPSC) from discarded umbilical cord and converting these pluripotent cells to CT, EVT and other lineages ..
  3. Mice Humanized with Hepatocytes and iPS Cells from Patients with Metabolic Diseas
    Stephen C Strom; Fiscal Year: 2010
    DESCRIPTION (provided by applicant): Specific Challenge Topic 14-DK-101, "Induced Pluripotent stem cells-cellular and humanized mouse" Somatic cells such as fibroblasts from patients with diseases can be used to create cell lines, ..
  4. Mice Humanized with Hepatocytes and iPS Cells from Patients with Metabolic Diseas
    Stephen Strom; Fiscal Year: 2009
    DESCRIPTION (provided by applicant): Specific Challenge Topic 14-DK-101, "Induced Pluripotent stem cells-cellular and humanized mouse" Somatic cells such as fibroblasts from patients with diseases can be used to create cell lines, ..
  5. Novel Imaging Approach to Monitor Chondrogenic Differentiation of iPS Cells
    HEIKE ELIZABETH DALDRUP LINK; Fiscal Year: 2010
    ..Stem cell transplants provide a potentially curative therapeutic option. Human induced pluripotent stem cells (hiPS cells) represent a novel cell type, which integrates advantages of human embryonic stem cells (hESCs)..
  6. Role of murine induced pluripotent stem cells on the correction of cardiac and sk
    Diego Fraidenraich; Fiscal Year: 2009
    ..These cells were named induced pluripotent stem cells (iPS cells). The iPS cells are similar to the ES cells...
  7. Role of murine induced pluripotent stem cells on the correction of cardiac and sk
    Diego Fraidenraich; Fiscal Year: 2010
    ..These cells were named induced pluripotent stem cells (iPS cells). The iPS cells are similar to the ES cells...
  8. Patient-Derived Stem Cells for Phosphoproteomic Profiling Neuropsychopathology
    Dieter A Wolf; Fiscal Year: 2011
    ..Recent advances have allowed the conversion of patient-specific human fibroblasts to human induced pluripotent stem cells (hIPSCs), cells which become sufficiently dedifferentiated to a primordial developmental stage that they ..
  9. Patient-Derived Stem Cells for Phosphoproteomic Profiling Neuropsychopathology
    Evan Snyder; Fiscal Year: 2009
    ..Recent advances have allowed the conversion of patient-specific human fibroblasts to human induced pluripotent stem cells (hIPSCs), cells which become sufficiently dedifferentiated to a primordial developmental stage that they ..
  10. Patient-Derived Stem Cells for Phosphoproteomic Profiling Neuropsychopathology
    Dieter A Wolf; Fiscal Year: 2010
    ..Recent advances have allowed the conversion of patient-specific human fibroblasts to human induced pluripotent stem cells (hIPSCs), cells which become sufficiently dedifferentiated to a primordial developmental stage that they ..
  11. Pluripotent Adult Spermatogonial Stem Cells: Prospective for Retinal Degeneration
    Nady Golestaneh; Fiscal Year: 2010
    ..stem cells for the first time and under defined culture conditions, we have reprogrammed the SSCs to pluripotent stem cells capable of differentiating into all three germ layers...
  12. Pluripotent Adult Spermatogonial Stem Cells: Prospective for Retinal Degeneration
    Nady Golestaneh; Fiscal Year: 2009
    ..stem cells for the first time and under defined culture conditions, we have reprogrammed the SSCs to pluripotent stem cells capable of differentiating into all three germ layers...
  13. Device for Hyperfast Scalable Vitrification of Germplasm in Large Volumes
    IGOR KATKOV; Fiscal Year: 2012
    ..As such, cryopreservation of samples such as semen, cord blood stem cells, and sufficient amounts of pluripotent stem cells and ovarian tissue is extremely difficult...
  14. Wnt Signaling in Hematopoietic Stem Cell Specification and Leukemia
    WILSON KENDRICK CLEMENTS; Fiscal Year: 2012
    ..Determining how embryonic stem cells (ESCs) or induced pluripotent stem cells (iPSCs) are directed to become tissue specific stem cells such HSCs is a key goal of regenerative medicine...
  15. Human Pluripotent Stem Cell Differentiation with Defined O2 & Protein Engagement
    ROBERT SAMUEL LANGER; Fiscal Year: 2010
    ..high-throughput microarray platform and culture vessels will be carried out for differentiation of human pluripotent stem cells to cardiomyocytes, which has been shown to benefit from hypoxic culture, as a model system...
  16. Testing the Therapeutic Potential of iPS Cells for Inherited Skin Diseases
    Jakub Tolar; Fiscal Year: 2012
    ..Therefore, we are proposing to develop stem-cell based therapies for EB using autologous induced pluripotent stem cells (iPSC) derived from skin cells harvested from the same EB patient...
  17. Derivation of cerebral cortical GABAergic interneurons from human iPS cells
    Lorenz P Studer; Fiscal Year: 2010
    ..Induced pluripotent stem cells (iPSCs) are an important potential source of human interneurons that could be used to address both genetic ..
  18. Derivation of cerebral cortical GABAergic interneurons from human iPS cells
    Stewart Anderson; Fiscal Year: 2009
    ..Induced pluripotent stem cells (iPSCs) are an important potential source of human interneurons that could be used to address both genetic ..
  19. Osteoclasts from pluripotent stem cells as vectors to modify bone marrow environm
    Hector L Aguila; Fiscal Year: 2010
    ..these experiments will test the hypothesis that myeloid cells of human origin derived from modified human pluripotent stem cells can localize to the right bone microenvironments, and will provide experimental systems for delivery ..
  20. Repair of Bone Defects with Human Autologous Pluripotent Very Small Embryonic lik
    Russell S Taichman; Fiscal Year: 2012
    ..Human VSELs are pluripotent stem cells involved in the normal turnover and regeneration of tissues and their circulating levels greatly increase ..
  21. iPS-derived hepatocytes as a model of familial hypercholesterolemia
    Max A Cayo; Fiscal Year: 2011
    DESCRIPTION (provided by applicant): The generation of induced pluripotent stem cells (iPSC) from adult somatic cells affords a novel approach the study and potential treatment of genetic diseases on a patient-specific level...
  22. Human Blood-Brain Barrier Model for CNS Drug Translation
    Eric V Shusta; Fiscal Year: 2010
    ..solution, we propose creating a blood-brain barrier model based on endothelial cells derived from human pluripotent stem cells (hPSCs)...
  23. Induced Pluripotent Stem Cells in Canine Muscular Dystrophy
    Martin K Childers; Fiscal Year: 2010
    ..For this purpose we propose to utilize cardiomyocytes differentiated in vitro from pluripotent stem cells. The recent demonstration that it is possible to generate induced pluripotent stem (iPS) cells from easily ..
  24. ATM Controls Genomic Stability in Pluripotent Stem Cells
    JASON MARK BECKTA; Fiscal Year: 2012
    DESCRIPTION (provided by applicant): Pluripotent stem cells (PSCs) harbor exceptional potential for developing therapeutic advances for many diseases. However, when cultured in vitro, PSCs commonly acquire genetic abnormalities (e.g...
  25. Novel therapies for Fanconi anemia
    DAVID A contact WILLIAMS; Fiscal Year: 2010
    ..One approach that could address this deficiency is the derivation of HSC from induced pluripotent stem cells (iPS) reprogrammed from other somatic tissues...
  26. Studying a Human Stem Cell Disease Using iPS Technology
    Steven E Artandi; Fiscal Year: 2010
    ..Develop molecular signatures for heart, vascular, lung, blood diseases by profiling reprogrammed induced pluripotent stem cells derived from affected individuals of defined genotype...
  27. PRF 10th Anniversary Workshop on Progeria - "From Bench to Bedside in a Decade"
    Leslie B Gordon; Fiscal Year: 2010
    ..heart disease, lamin biology, and Progeria, as well as experts on cutting edge research such as induced pluripotent stem cells and high throughput assay systems, will contribute to the workshop from both the basic and clinical ..
  28. Improving Epigenetic-based Cell Reprogramming with Proteasome Inhibition
    RACHEL contact POWER; Fiscal Year: 2010
    ..raised the possibility that alternative strategies could be developed on an industrial scale to produce pluripotent stem cells without using embryos or genetic manipulations...
  29. Novel Reprogrammed Cells for Differentiation into Cardiomyocytes
    RALPH SNODGRASS; Fiscal Year: 2010
    ..raised the possibility that alternative strategies could be developed on an industrial scale to produce pluripotent stem cells without using embryos...
  30. INDUCIBLE PLURIPOTENT STEM CELLS AND KIDNEY REGENERATION
    JOSEPH VINCENT BONVENTRE; Fiscal Year: 2010
    ..The purpose of this study is to develop strategies for transforming stem cells into cells of the kidney, with the ultimate goal of regenerating kidney tissue to treat patients with CKD. ..
  31. INDUCIBLE PLURIPOTENT STEM CELLS AND KIDNEY REGENERATION
    Joseph Bonventre; Fiscal Year: 2009
    ..The purpose of this study is to develop strategies for transforming stem cells into cells of the kidney, with the ultimate goal of regenerating kidney tissue to treat patients with CKD. ..
  32. Mechanisms of Testosterone Effects on Body Composition
    Shalendar Bhasin; Fiscal Year: 2007
    ..validated in vitro models of adult, mesenchymal, stem cell commitment and differentiation, namely, adult, pluripotent stem cells isolated from lipoaspirates, and muscle stem cells isolated from regenerating skeletal muscle of adult ..
  33. Mechanisms of Testosterone Effects on Body Composition
    Shalender Bhasin; Fiscal Year: 2004
    ..validated in vitro models of adult, mesenchymal, stem cell commitment and differentiation, namely, adult, pluripotent stem cells isolated from lipoaspirates, and muscle stem cells isolated from regenerating skeletal muscle of adult ..
  34. Cardiac Progenitor Patch for Treatment of Myocardial Infarction
    Nenad Bursac; Fiscal Year: 2010
    ..or cardiovascular progenitor cells (ESC-CPCs), and recently, cardiac progenitors isolated from induced pluripotent stem cells (iPSCs)...
  35. Mechanisms of Testosterone Effects on Body Composition
    Shalender Bhasin; Fiscal Year: 2005
    ..validated in vitro models of adult, mesenchymal, stem cell commitment and differentiation, namely, adult, pluripotent stem cells isolated from lipoaspirates, and muscle stem cells isolated from regenerating skeletal muscle of adult ..
  36. Mechanisms of Testosterone Effects on Body Composition
    Shalender Bhasin; Fiscal Year: 2006
    ..validated in vitro models of adult, mesenchymal, stem cell commitment and differentiation, namely, adult, pluripotent stem cells isolated from lipoaspirates, and muscle stem cells isolated from regenerating skeletal muscle of adult ..
  37. Modeling complex diseases using iPSC-derived skin constructs
    Angela M Christiano; Fiscal Year: 2012
    ..aim o establish a skin construct composed entirely of fibroblasts and keratinocytes derived from induced pluripotent stem cells (iPSCs), to produce an unlimited supply of disease- specific donor cells for use in skin constructs, which ..
  38. Generating Human Intestinal Organoids with an ENS.
    James M Wells; Fiscal Year: 2012
    ..human intestinal tissue "organoids" (HIOs) through directed differentiation of human embryonic and induced pluripotent stem cells (collectively called PSCs)...
  39. Cellular dissection of herpes simplex encephalitis with iPS cells
    Jean Laurent Casanova; Fiscal Year: 2010
    ..and NEMO) and from patients with other inborn errors of IFN immunity (mutation in TYK2) to derive induced pluripotent stem cells (iPSCs). These iPSCs will then be differentiated into neurons, oligodendrocytes, and astrocytes...
  40. HUMAN CANCER--ORIGIN AND GENETIC MARKERS
    PHILIP FIALKOW; Fiscal Year: 1993
    ..CML) and many ANLLs have a multistep pathogenesis in which clonal proliferation of genetically unstable pluripotent stem cells antedates the development of characteristic cytogenetic abnormalities such as the Philadelphia chromosome (..
  41. Protein-modified Hydrogels for Expansion of Human Embryonic Stem Cells
    TERRY TANDESKI; Fiscal Year: 2009
    The realization of the remarkable potential of human embryonic stem cells or induced pluripotent stem cells to treat human disease cannot occur unless new animal-free methods for the expansion of undifferentiated stem cells are developed...
  42. Development of normal and dystrophic canine iPS lines
    COLIN BISHOP; Fiscal Year: 2009
    ..The recent demonstration that is possible to generate autologous, pluripotent stem cells (iPS) from skin fibroblasts, by relatively simple reprogramming steps is a major scientific advance which ..
  43. Human iPS/ES Cell-Based Models for Predictive Neural Toxicity and Teratogenicity
    JAMES ALEXANDER THOMSON; Fiscal Year: 2012
    ..progress has been made in the derivation of many of the basic cellular components of the brain from human pluripotent stem cells (ES and iPS cells), but these advances have yet to be applied to predictive toxicology...
  44. Targeting SMN2 Alternative Splicing for the Treatment of Spinal Muscular Atrophy
    Michelle L Hastings; Fiscal Year: 2010
    ..Aim 1 of this study is to use induced pluripotent stem cells derived from SMA patients to determine the effectiveness of splicing effector molecules, such as the ..
  45. DIFFERENTIATION OF ENDOCRINE CELL LINEAGES IN GI TRACT
    Andrew Leiter; Fiscal Year: 2000
    ..The Principal Investigator has preliminary data indicating that pluripotent stem cells give rise to PYY progenitors that then differentiate into other endocrine cells...
  46. DIFFERENTIATION OF ENDOCRINE CELL LINEAGES IN GI TRACT
    Andrew Leiter; Fiscal Year: 2001
    ..The Principal Investigator has preliminary data indicating that pluripotent stem cells give rise to PYY progenitors that then differentiate into other endocrine cells...
  47. DIFFERENTIATION OF ENDOCRINE CELL LINEAGES IN GI TRACT
    Andrew Leiter; Fiscal Year: 1999
    ..The Principal Investigator has preliminary data indicating that pluripotent stem cells give rise to PYY progenitors that then differentiate into other endocrine cells...
  48. Novel assay for functional capacities of pluripotent stem cells
    ROGER ARNOLD PEDERSEN; Fiscal Year: 2012
    DESCRIPTION (provided by applicant): Pluripotent stem cells are capable of differentiating into all body tissues and are able to renew themselves through unlimited proliferation...
  49. REGULATION OF TDT EXPRESSION DURING LYMPHOPOIESIS
    STEPHEN SMALE; Fiscal Year: 1991
    Hematopoiesis is a complex developmental process through which pluripotent stem cells in mammalian fetal liver or adult bone marrow give rise to several types of terminally differentiated blood cells, including cells in this pathway are ..
  50. Role of OCT4 in reprogramming human skin keratinocytes
    Jackie R Bickenbach; Fiscal Year: 2011
    ..sufficient to reactivate endogenous embryonic factors required to reprogram human skin keratinocytes into pluripotent stem cells. To test our hypothesis, we propose the following Specific Aims: 1) Reprogram adult human skin ..
  51. Role of OCT4 in reprogramming human skin keratinocytes
    Jackie Bickenbach; Fiscal Year: 2009
    ..sufficient to reactivate endogenous embryonic factors required to reprogram human skin keratinocytes into pluripotent stem cells. To test our hypothesis, we propose the following Specific Aims: 1) Reprogram adult human skin ..
  52. Role of OCT4 in reprogramming human skin keratinocytes
    Jackie R Bickenbach; Fiscal Year: 2010
    ..sufficient to reactivate endogenous embryonic factors required to reprogram human skin keratinocytes into pluripotent stem cells. To test our hypothesis, we propose the following Specific Aims: 1) Reprogram adult human skin ..
  53. IN VIVO MODEL FOR HUMAN STEM CELLS
    Donald Kohn; Fiscal Year: 1999
    ..We will then use this system to study methods to achieve maximal gene transduction of human pluripotent stem cells. We will also use this system to compare adeno-associated virus (AAV) vectors to retroviral vectors...
  54. Human Pluripotent Stem Cell and Progenitor Models of Cardiac and Blood Diseases
    LEONARD IRA ZON; Fiscal Year: 2010
    ..by applicant): Our groups have common interests in the development of improved techniques for generating pluripotent stem cells, directing their differentiation into relevant tissues, and in disease modeling in two major systems of ..
  55. Human Pluripotent Stem Cell and Progenitor Models of Cardiac and Blood Diseases
    George Daley; Fiscal Year: 2009
    ..by applicant): Our groups have common interests in the development of improved techniques for generating pluripotent stem cells, directing their differentiation into relevant tissues, and in disease modeling in two major systems of ..
  56. Transplantability of induced pluripotent stem cells for skeletal tissues
    Christopher Niyibizi; Fiscal Year: 2010
    ..application are to assess migration, engraftment and differentiation of cells derived from induced pluripotent stem cells (iPSC) into skeletal tissues following systemic transplantation...
  57. Multipotent stem cells for tissue regeneration
    Song Li; Fiscal Year: 2010
    ..The generation of induced pluripotent stem cells (iPSCs) bypasses the ethical and immune rejection issues of human embryonic stem cells (hESCs), and ..
  58. NON-APOPTOTIC ACTION OF CASPASES IN PLURIPOTENT EMBRYONIC STEM CELLS
    THOMAS ZWAKA; Fiscal Year: 2009
    ..mediators of programmed cell death, especially caspase-3, also mediate the fate decisions affecting pluripotent stem cells. In Aim 1 I will dissect the functional roles of caspase-3 and it's activating caspase in the fate of ..
  59. Antibodies Targeting Novel Surface Antigens on Pluripotent Stem Cell Derivatives
    David Larocca; Fiscal Year: 2009
    ..current limited knowledge of surface markers that define the various cell types that differentiate from pluripotent stem cells. As pluripotent stem cells differentiate there is heterogeneity both spatially, in the cell types formed ..
  60. Generation of Human iPS Cells via Non-integrating Vectors
    Wen Shu Wu; Fiscal Year: 2010
    ..A particularly exciting method for the generation of patient-specific (or isogenic) pluripotent stem cells is somatic cell reprogramming...
  61. Generation of Human iPS Cells via Non-integrating Vectors
    Wen Shu Wu; Fiscal Year: 2009
    ..A particularly exciting method for the generation of patient-specific (or isogenic) pluripotent stem cells is somatic cell reprogramming...
  62. Translating Dosage Compensation to Trisomy
    Jeanne Bentley Lawrence; Fiscal Year: 2010
    ..one X chromosome in mammalian female embryos to "dosage compensate" trisomy 21, primarily in induced pluripotent stem cells which have strong therapeutic potential...
  63. Human Induced Pluripotent Stem Cells To Investigate Inherited Skin Diseases
    LOUISE CHOW; Fiscal Year: 2009
    ..Success in these experiments would serve as proof-of-principle that iPS cells can be used widely by researchers to study genetic skin diseases and to test for new therapeutic approaches. ..
  64. STUDIES ON PANCREATIC HORMONES
    DONALD STEINER; Fiscal Year: 1991
    ..differentiation and growth of islet cells using clonally derived insulinoma lines having the properties of pluripotent stem cells. (8) Studies on the structure of the insulin receptor with special emphasis on the molecular mechanisms of ..
  65. PANCREATIC HORMONES
    DONALD STEINER; Fiscal Year: 1990
    ..differentiation and growth of islet cells using clonally derived insulinoma lines having the properties of pluripotent stem cells. (8) Studies on the structure of the insulin receptor with special emphasis on the molecular mechanisms of ..
  66. Maturation of Normal and Disease-specific Human Stem Cell-derived Cardiomyocytes
    Huei Sheng Vincent Chen; Fiscal Year: 2010
    ..Recent progress in cellular reprogramming of various somatic cell types into induced pluripotent stem cells (iPSCs) opened the door for developing patient-specific, cell-based therapies...
  67. In vitro differentiation of RAG1-mutated iPS cells and correction by meganuclease
    Luigi Daniele Notarangelo; Fiscal Year: 2010
    ..However, fibroblasts can be reprogrammed in vitro into induced pluripotent stem cells (iPSCs) through virus-mediated transduction of a combination of transcription factors...
  68. 2010 Gordon Research Conference on Endothelial Cell Phenotypes in Health and Dise
    Edward Conway; Fiscal Year: 2010
    ..inducible pluripotent stem cells and endothelial progenitor cells, 2...
  69. LATENCY AND REACTIVATION OF CYTOMEGALOVIRUS AFTER BONE MARROW TRANSPLANTATION
    Edward Mocarski; Fiscal Year: 2002
    ..to murine CMV in mice transplanted with allogeneic versus congenic hematopoietic cell transplants with pluripotent stem cells plus purified lymphoid subsets...
  70. Heterochromatin instability and misregulation of noncoding RNA in cancer
    Dawn M Carone; Fiscal Year: 2010
    ..Surprisingly, our recent findings also indicate that centromeric heterochromatin of pluripotent stem cells is structurally distinct from that of lineage committed somatic cells...
  71. Rest-Activated Program of Gene Expression in Ischemia
    R Suzanne Zukin; Fiscal Year: 2010
    ..factor that is widely expressed during embryogenesis and plays a strategic role in the transition from pluripotent stem cells to neurons...
  72. Rest-Activated Program of Gene Expression in Ischemia
    R Suzanne Zukin; Fiscal Year: 2010
    ..factor that is widely expressed during embryogenesis and plays a strategic role in the transition from pluripotent stem cells to neurons...
  73. In Vivo Control and Functional Visualization of Stem Cell-Driven CNS Regeneration
    Jin Hyung Lee; Fiscal Year: 2010
    ..ability of stem cells to regenerate non-dividing cells, as well as recent development of induced pluripotent stem cells (IPSC) gives fresh impetus for a whole new class of innovative treatments where damaged neural circuitry ..
  74. Development of iPS cells to study craniometaphyseal dysplasia in humans
    Alexander Lichtler; Fiscal Year: 2009
    ..Therefore, we propose to create a source of induced pluripotent stem cells (iPS) from CMD patients and control individuals, which can be differentiated into cells involved in bone ..
  75. Frontiers in Stem Cells and Regeneration
    Gerald Schatten; Fiscal Year: 2009
    DESCRIPTION (provided by applicant): Pluripotent stem cells and regenerative medicine are rapidly emerging fields with enormous biomedical implications across the entire spectrum of NIH's clinical, translational, biomedical and ..
  76. Multipotent heart cell progenitor lineages in cardiac development and disease
    Kenneth Chien; Fiscal Year: 2009
    ..Islet-1 cardiovascular progenitors from both murine and human embryonic stem (ES) cells and inducible pluripotent stem cells (iPS)...
  77. Frontiers in Stem Cells and Regeneration
    Gerald Schatten; Fiscal Year: 2010
    DESCRIPTION (provided by applicant): Pluripotent stem cells and regenerative medicine are rapidly emerging fields with enormous biomedical implications across the entire spectrum of NIH's clinical, translational, biomedical and ..
  78. Aging and Reprogramming of Somatic Cells to Pluripotency
    Peter Hornsby; Fiscal Year: 2009
    ..Reprogramming of somatic cells to induced pluripotent stem cells (iPS cells) provides the opportunity to test whether aging at the cell level is reversible and whether the ..
  79. Use of 3-D Culture and Stretch to Develop Lung from MSCs, ESCs, and iPS
    Daniel J Weiss; Fiscal Year: 2010
    ..approaches utilizing embryonic stem cells (ESCs), adult mesenchymal stem cells (MSCs), or induced pluripotent stem cells (iPS) cultured in three-dimensional culture matrices can regenerate functional lung tissue for replacement ..
  80. Characterizing blood progenitor cells differentiated from human iPS and ES cells
    Linzhao Cheng; Fiscal Year: 2009
    ..Differentiated Heart, Lung, and Blood Cells Derived by Reprogramming Human Embryonic and Induced Pluripotent Stem Cells"...
  81. Cell-fate determinants of yeast pseudohyphal growth
    Beverly Errede; Fiscal Year: 2004
    ..switch is fundamentally the same as those that occur during the production of distinct lineages from pluripotent stem cells in humans...
  82. Cardiac Induction by Small Molecule BMP Inhibitors
    Charles C Hong; Fiscal Year: 2010
    ..have introduced the prospect of replacing damaged heart tissues with healthy cardiomyocytes derived from pluripotent stem cells. However, realizing the full therapeutic potential of stem cells faces numerous hurdles, including the ..
  83. Cell-fate determinants of yeast pseudohyphal growth
    Beverly Errede; Fiscal Year: 2003
    ..switch is fundamentally the same as those that occur during the production of distinct lineages from pluripotent stem cells in humans...
  84. Cell-fate determinants of yeast pseudohyphal growth
    Beverly Errede; Fiscal Year: 2005
    ..switch is fundamentally the same as those that occur during the production of distinct lineages from pluripotent stem cells in humans...
  85. Use of Clenbuterol for Enhancement of Stem Cell Therapy
    Richard Zeman; Fiscal Year: 2002
    ..The proposed studies are an outgrowth of previous work in which Beta2-agonists or transplantation of adult pluripotent stem cells into the contusion site enhanced recovery of locomotor function following SCI in rats...
  86. Gene Targeting and Integration by Adeno-Associated Virus Vectors
    David Russell; Fiscal Year: 2009
    ..employed can be used therapeutically when transplantation therapies are developed for patient-derived pluripotent stem cells. Second, using modern sequencing technologies we will determine where AAV-mediated gene targeting and non-..
  87. Differential Effects of BMPs on the Healing of Craniofacial Defects
    Russell Reid; Fiscal Year: 2009
    ..A means of providing large quantities of bone would be to transfer engineered, pluripotent stem cells that could induce bony regeneration...
  88. Characterization of human hematopoietic and endodermal progenitors derived from i
    Darrell Kotton; Fiscal Year: 2009
    ..This proposal develops induced pluripotent stem cells from skin biopsies of normal controls as well as individuals with these diseases...
  89. Double-Gated Selection of Ligands that Target Surface Markers of Differentiation
    David Larocca; Fiscal Year: 2009
    ..to pluripotent cells with the same properties as embryonic stem cells have increased the availability of pluripotent stem cells for research and may ultimately provide cells for clinical applications is they can be proven safe...
  90. Molecular Dissection of the Hematopoeic Microenvironment
    Kateri Moore; Fiscal Year: 2003
    ..It has been shown that these pluripotent stem cells reside in discrete niches within a hematopoietic organ...
  91. Molecular Dissection of the Hematopoeic Microenvironment
    Kateri Moore; Fiscal Year: 2004
    ..It has been shown that these pluripotent stem cells reside in discrete niches within a hematopoietic organ...
  92. ORIGIN AND DIFFERENTIATION OF NATURAL KILLER CELLS
    Vinay Kumar; Fiscal Year: 1993
    ..Populations enriched for Nkp activity (e.g. Ly6+, Lineage-, CD43+, Fall 3+) will be tested for pluripotent stem cells, and transplantable T and B cell progenitors...
  93. Molecular Dissection of the Hematopoeic Microenvironment
    Kateri Moore; Fiscal Year: 2002
    ..It has been shown that these pluripotent stem cells reside in discrete niches within a hematopoietic organ...
  94. Molecular Dissection of the Hematopoeic Microenvironment
    Kateri Moore; Fiscal Year: 2003
    ..It has been shown that these pluripotent stem cells reside in discrete niches within a hematopoietic organ...