vero cells

Summary

Summary: A CELL LINE derived from the kidney of the African green (vervet) monkey, (CERCOPITHECUS AETHIOPS) used primarily in virus replication studies and plaque assays.

Top Publications

  1. pmc Epidemiologic and virologic investigation of hand, foot, and mouth disease, southern Vietnam, 2005
    Phan Van Tu
    Pasteur Institute, Ho Chi Minh City, Vietnam
    Emerg Infect Dis 13:1733-41. 2007
  2. ncbi Endosomal proteolysis of the Ebola virus glycoprotein is necessary for infection
    Kartik Chandran
    Department of Medicine, Brigham and Women s Hospital and Harvard Medical School, Boston, MA 02115, USA
    Science 308:1643-5. 2005
  3. ncbi Identification and characterization of a cross-neutralization epitope of Enterovirus 71
    Chia Chyi Liu
    Vaccine R and D Center, National Health Research Institutes, Zhunan Town, Miaoli County 350, Taiwan
    Vaccine 29:4362-72. 2011
  4. pmc A mouse model for Chikungunya: young age and inefficient type-I interferon signaling are risk factors for severe disease
    Thérèse Couderc
    Groupe Microorganismes et Barrières de l Hôte, Institut Pasteur, Paris, France
    PLoS Pathog 4:e29. 2008
  5. ncbi Herpes simplex virus-1 entry into cells mediated by a novel member of the TNF/NGF receptor family
    R I Montgomery
    Northwestern University Medical School, Department of Microbiology Immunology, Chicago, Illinois 60611, USA
    Cell 87:427-36. 1996
  6. pmc Role of endosomal cathepsins in entry mediated by the Ebola virus glycoprotein
    Kathryn Schornberg
    Department of Microbiology, University of Virginia, 1300 Jefferson Park Ave, Charlottesville, Virginia 22908 0734, USA
    J Virol 80:4174-8. 2006
  7. pmc Inhibition of interferon signaling by dengue virus
    Jorge L Munoz-Jordan
    Department of Microbiology, Mount Sinai School of Medicine, One Gustave Levy Place, New York, NY 10029
    Proc Natl Acad Sci U S A 100:14333-8. 2003
  8. ncbi Structural basis of West Nile virus neutralization by a therapeutic antibody
    Grant E Nybakken
    Department of Pathology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Nature 437:764-9. 2005
  9. pmc Roles for endocytosis and low pH in herpes simplex virus entry into HeLa and Chinese hamster ovary cells
    Anthony V Nicola
    Medical Virology Section, Laboratory of Clinical Investigation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Virol 77:5324-32. 2003
  10. pmc Shiga toxin subtypes display dramatic differences in potency
    Cynthia A Fuller
    Molecular Genetics, Biochemistry, and Microbiology, Room 3109, 231 Albert Sabin Way, ML 524, University of Cincinnati, Cincinnati, OH 45267 0524, USA
    Infect Immun 79:1329-37. 2011

Detail Information

Publications401 found, 100 shown here

  1. pmc Epidemiologic and virologic investigation of hand, foot, and mouth disease, southern Vietnam, 2005
    Phan Van Tu
    Pasteur Institute, Ho Chi Minh City, Vietnam
    Emerg Infect Dis 13:1733-41. 2007
    ..In the second half of the year, viruses belonging to subgenogroup C5 predominated during a period of higher HEV71 activity...
  2. ncbi Endosomal proteolysis of the Ebola virus glycoprotein is necessary for infection
    Kartik Chandran
    Department of Medicine, Brigham and Women s Hospital and Harvard Medical School, Boston, MA 02115, USA
    Science 308:1643-5. 2005
    ..CatB and CatB/CatL inhibitors diminish the multiplication of infectious EboV-Zaire in cultured cells and may merit investigation as anti-EboV drugs...
  3. ncbi Identification and characterization of a cross-neutralization epitope of Enterovirus 71
    Chia Chyi Liu
    Vaccine R and D Center, National Health Research Institutes, Zhunan Town, Miaoli County 350, Taiwan
    Vaccine 29:4362-72. 2011
    ..Thus, VP2-28 could be used as the surrogate biomarker in the potency testing of candidate EV71 vaccines...
  4. pmc A mouse model for Chikungunya: young age and inefficient type-I interferon signaling are risk factors for severe disease
    Thérèse Couderc
    Groupe Microorganismes et Barrières de l Hôte, Institut Pasteur, Paris, France
    PLoS Pathog 4:e29. 2008
    ..The development of a permissive small animal model will expedite the testing of future vaccines and therapeutic candidates...
  5. ncbi Herpes simplex virus-1 entry into cells mediated by a novel member of the TNF/NGF receptor family
    R I Montgomery
    Northwestern University Medical School, Department of Microbiology Immunology, Chicago, Illinois 60611, USA
    Cell 87:427-36. 1996
    ..The contribution of HVEM to HSV entry into human cells was demonstrable in activated T cells. HVEM, the first identified mediator of HSV entry, is a new member of the TNF/NGF receptor family...
  6. pmc Role of endosomal cathepsins in entry mediated by the Ebola virus glycoprotein
    Kathryn Schornberg
    Department of Microbiology, University of Virginia, 1300 Jefferson Park Ave, Charlottesville, Virginia 22908 0734, USA
    J Virol 80:4174-8. 2006
    ..These and other results support a model in which CatB and CatL prime GP by generating a 19-kDa intermediate that can be acted upon by an as yet unidentified endosomal/lysosomal enzyme to trigger fusion...
  7. pmc Inhibition of interferon signaling by dengue virus
    Jorge L Munoz-Jordan
    Department of Microbiology, Mount Sinai School of Medicine, One Gustave Levy Place, New York, NY 10029
    Proc Natl Acad Sci U S A 100:14333-8. 2003
    ..This protein, encoded by a positive strand RNA virus, is implicated as an IFN-signaling inhibitor...
  8. ncbi Structural basis of West Nile virus neutralization by a therapeutic antibody
    Grant E Nybakken
    Department of Pathology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Nature 437:764-9. 2005
    ..Collectively, our results suggest that a vaccine strategy targeting the dominant DIII epitope may elicit safe and effective immune responses against flaviviral diseases...
  9. pmc Roles for endocytosis and low pH in herpes simplex virus entry into HeLa and Chinese hamster ovary cells
    Anthony V Nicola
    Medical Virology Section, Laboratory of Clinical Investigation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Virol 77:5324-32. 2003
    Herpes simplex virus (HSV) infection of many cultured cells, e.g., Vero cells, can be initiated by receptor binding and pH-neutral fusion with the cell surface...
  10. pmc Shiga toxin subtypes display dramatic differences in potency
    Cynthia A Fuller
    Molecular Genetics, Biochemistry, and Microbiology, Room 3109, 231 Albert Sabin Way, ML 524, University of Cincinnati, Cincinnati, OH 45267 0524, USA
    Infect Immun 79:1329-37. 2011
    ..In both RPTECs and Vero cells, Stx2a, Stx2d, and elastase-cleaved Stx2d were at least 25 times more potent than Stx2b and Stx2c...
  11. ncbi Selection and characterization of vaccine strain for Enterovirus 71 vaccine development
    Jui Yuan Chang
    Vaccine R and D Center, National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Zhunan Township, Miaoli County 350, Taiwan
    Vaccine 30:703-11. 2012
    ..Therefore, these results provide valuable information for cell-based EV71 vaccine development...
  12. pmc The NS5 protein of the virulent West Nile virus NY99 strain is a potent antagonist of type I interferon-mediated JAK-STAT signaling
    Maudry Laurent-Rolle
    Laboratory of Virology, Rocky Mountain Laboratories, 903 S Fourth St, Hamilton, MT 59840, USA
    J Virol 84:3503-15. 2010
    ..Thus, a naturally occurring mutation is associated with the function of NS5 in IFN antagonism and may influence virulence of WNV field isolates...
  13. pmc The endoplasmic reticulum provides the membrane platform for biogenesis of the flavivirus replication complex
    Leah K Gillespie
    Department of Microbiology, La Trobe University, Bundoora, Melbourne 3086, Australia
    J Virol 84:10438-47. 2010
    ..In addition, we provide data that indicate that a majority of the viral RNA species housed within the RC is in a double-stranded RNA (dsRNA) form...
  14. ncbi Image-guided radiovirotherapy for multiple myeloma using a recombinant measles virus expressing the thyroidal sodium iodide symporter
    David Dingli
    Molecular Medicine Program, Mayo Clinic and Foundation, Rochester, MN 55905, USA
    Blood 103:1641-6. 2004
    ..Testing in other radiosensitive cancers is warranted...
  15. pmc West Nile virus replication requires fatty acid synthesis but is independent on phosphatidylinositol-4-phosphate lipids
    Miguel A Martín-Acebes
    Departamento de Biotecnologia, Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria INIA, Madrid, Spain
    PLoS ONE 6:e24970. 2011
    ..Similar features were found when infection by other flavivirus, the Usutu virus (USUV), was analyzed. These features of WNV replication could help to design specific antiviral approaches against WNV and other related flaviviruses...
  16. pmc Monoclonal antibodies that bind to domain III of dengue virus E glycoprotein are the most efficient blockers of virus adsorption to Vero cells
    W D Crill
    Arbovirus Disease Branch, Division of Vector Borne Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, U S Department of Health and Human Services, Fort Collins, Colorado 80522, USA
    J Virol 75:7769-73. 2001
    ..set of anti-dengue (DEN) virus E-glycoprotein-specific monoclonal antibodies (MAbs) to block virus adsorption to Vero cells. In contrast to previous studies, the binding sites of these MAbs were localized to one of three structural ..
  17. pmc Endoproteolytic processing of the lymphocytic choriomeningitis virus glycoprotein by the subtilase SKI-1/S1P
    Winfried R Beyer
    Heinrich Pette Institut fur experimentelle Virologie und Immunologie an der Universitat Hamburg, D 20251 Hamburg, Germany
    J Virol 77:2866-72. 2003
    ..In agreement with the identified consensus motif, we show that the cellular subtilase SKI-1/S1P cleaves LCMV GP...
  18. pmc A monoclonal immunoglobulin G antibody directed against an immunodominant linear epitope on the ricin A chain confers systemic and mucosal immunity to ricin
    Lori M Neal
    Division of Infectious Disease, Wadsworth Center, New York State Department of Health, Albany, New York 12208, USA
    Infect Immun 78:552-61. 2010
    ..The potential of GD12 to serve as a therapeutic following ricin challenge was not explored in this study...
  19. pmc Ebolavirus is internalized into host cells via macropinocytosis in a viral glycoprotein-dependent manner
    Asuka Nanbo
    Influenza Research Institute, Department of Pathological Sciences, School of Veterinary Medicine, University of Wisconsin Madison, Madison, Wisconsin, United States of America
    PLoS Pathog 6:e1001121. 2010
    ..These findings provide new insights into the lifecycle of Ebolavirus and may aid in the development of therapeutics for Ebolavirus infection...
  20. pmc Rab7 associates with early endosomes to mediate sorting and transport of Semliki forest virus to late endosomes
    Andreas Vonderheit
    Institute of Biochemistry, Swiss Federal Institute of Technology, Zurich, Switzerland
    PLoS Biol 3:e233. 2005
    ..We concluded that Rab7 is recruited to early endosomes, where it forms distinct domains that mediate cargo sorting as well as the formation of late-endosome-targeted transport vesicles...
  21. pmc Caspase 3 activation is essential for efficient influenza virus propagation
    Walter J Wurzer
    Institute of Molecular Medicine IMM, Heinrich Heine Universitat, Universitatsstrasse 1, D 40225 Dusseldorf, Germany
    EMBO J 22:2717-28. 2003
    ..Our findings indicate that caspase 3 activation during the onset of apoptosis is a crucial event for efficient influenza virus propagation...
  22. ncbi Use of the red fluorescent protein as a marker of Kaposi's sarcoma-associated herpesvirus lytic gene expression
    Jeffrey Vieira
    Department of Laboratory Medicine, University of Washington, Seattle, WA 98109 8070, USA
    Virology 325:225-40. 2004
    ..rKSHV.219 from JSC-1 cells was used to infect Vero cells for purification of the recombinant virus. Vero cells were also used for the production of rKSHV...
  23. pmc Maturation of West Nile virus modulates sensitivity to antibody-mediated neutralization
    Steevenson Nelson
    Viral Pathogenesis Section, Laboratory of Viral Diseases, National Institutes of Health, Bethesda, Maryland, United States of America
    PLoS Pathog 4:e1000060. 2008
    ..Thus, in addition to a role in facilitating viral entry, changes in E protein arrangement associated with maturation modulate neutralization sensitivity and introduce an additional layer of complexity into humoral immunity against WNV...
  24. pmc Blocking of interferon-induced Jak-Stat signaling by Japanese encephalitis virus NS5 through a protein tyrosine phosphatase-mediated mechanism
    Ren Jye Lin
    Institute of Biomedical Sciences, Academia Sinica, Nankang, Taipei 11529, Taiwan, Republic of China
    J Virol 80:5908-18. 2006
    ..We suggest that JEV NS5 is an IFN antagonist and that it may play a role in blocking IFN-stimulated Jak-Stat signaling via activation of PTPs during JEV infection...
  25. ncbi Conserved receptor-binding domains of Lake Victoria marburgvirus and Zaire ebolavirus bind a common receptor
    Jens H Kuhn
    Department of Microbiology and Molecular Genetics, Harvard Medical School, New England Primate Research Center, Southborough, Massachusetts 01772, USA
    J Biol Chem 281:15951-8. 2006
    ..These studies identify the receptor-binding domains of both viruses, indicate that these viruses utilize a common receptor, and suggest that a single small molecule or vaccine can be developed to inhibit infection of all filoviruses...
  26. ncbi Cholesterol manipulation by West Nile virus perturbs the cellular immune response
    Jason M Mackenzie
    School of Molecular and Microbial Sciences, University of Queensland, Brisbane, Queensland 4072, Australia
    Cell Host Microbe 2:229-39. 2007
    ....
  27. pmc Assembly and maturation of the flavivirus Kunjin virus appear to occur in the rough endoplasmic reticulum and along the secretory pathway, respectively
    J M MacKenzie
    Clinical Medical Virology Centre, University of Queensland, St Lucia, Queensland 4072, Australia
    J Virol 75:10787-99. 2001
    ..The results suggest that the assembly of virions occurs within the lumen of the RER and that subsequent maturation occurs via the secretory pathway...
  28. pmc Dengue virus ensures its fusion in late endosomes using compartment-specific lipids
    Elena Zaitseva
    Section on Membrane Biology, Laboratory of Cellular and Molecular Biophysics, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, United States of America
    PLoS Pathog 6:e1001131. 2010
    ....
  29. pmc An attenuated strain of enterovirus 71 belonging to genotype a showed a broad spectrum of antigenicity with attenuated neurovirulence in cynomolgus monkeys
    Minetaro Arita
    Department of Virology II, National Institute of Infectious Diseases, 4 7 1 Gakuen, Musashimurayama shi, Tokyo 208 0011, Japan
    J Virol 81:9386-95. 2007
    ..In the CNS, EV71(S1-3') was isolated only from the spinal cord. These results indicate that EV71(S1-3') acts as an effective antigen, although this attenuated strain was still neurotropic when inoculated via the intravenous route...
  30. pmc Purification and characterization of enterovirus 71 viral particles produced from vero cells grown in a serum-free microcarrier bioreactor system
    Chia Chyi Liu
    Vaccine Research and Development Center, National Health Research Institutes, Zhunan Town, Miaoli County, Taiwan
    PLoS ONE 6:e20005. 2011
    ..Enterovirus 71 (EV71) infections manifest most commonly as a childhood exanthema known as hand-foot-and-mouth disease (HFMD) and can cause neurological disease during acute infection...
  31. pmc Ultrastructure of Kunjin virus-infected cells: colocalization of NS1 and NS3 with double-stranded RNA, and of NS2B with NS3, in virus-induced membrane structures
    E G Westaway
    Sir Albert Sakzewski Virus Research Centre, Royal Children s Hospital, Brisbane, Australia
    J Virol 71:6650-61. 1997
    The subcellular location of the nonstructural proteins NS1, NS2B, and NS3 in Vero cells infected with the flavivirus Kunjin was investigated using indirect immunofluorescence and cryoimmunoelectron microscopy with monospecific antibodies...
  32. pmc Cellular entry of ebola virus involves uptake by a macropinocytosis-like mechanism and subsequent trafficking through early and late endosomes
    Mohammad F Saeed
    Department of Microbiology and Immunology, The University of Texas Medical Branch, Galveston, Texas, United States of America
    PLoS Pathog 6:e1001110. 2010
    ..similar virus-like particles in specific infection and entry assays to demonstrate that in HEK293T and Vero cells internalization of ZEBOV is independent of clathrin, caveolae, and dynamin...
  33. pmc Identification of a putative coreceptor on Vero cells that participates in dengue 4 virus infection
    J J Martínez-Barragán
    Departamento de Patologia Experimental, Centro de Investigacion y de Estudios Avanzados del IPN, Mexico City 07360, Mexico
    J Virol 75:7818-27. 2001
    ..In this study we searched for molecules other than HS that could participate in dengue virus infection of Vero cells. Labeled dengue 4 virus bound with high affinity to two molecules of 74 and 44 kDa...
  34. ncbi A murine oral enterovirus 71 infection model with central nervous system involvement
    Yi Chun Chen
    Department of Microbiology, College of Medicine, National Cheng Kung University, Tainan, Taiwan, Republic of China
    J Gen Virol 85:69-77. 2004
    ..Leukocyte infiltration was evident in the spinal cord and brain stem. Furthermore, EV71 virus could be transmitted to littermates within the same cage...
  35. pmc Chimeric alphavirus vaccine candidates for chikungunya
    Eryu Wang
    Center for Biodefense and Emerging Infectious Diseases, and Sealy Center for Vaccine Development, University of Texas Medical Branch, Galveston, TX 77555, United States
    Vaccine 26:5030-9. 2008
    ..Vaccinated mice were fully protected against disease and viremia after CHIKV challenge...
  36. pmc Cellular entry of hantaviruses which cause hemorrhagic fever with renal syndrome is mediated by beta3 integrins
    I N Gavrilovskaya
    Department of Medicine, State University of New York at Stony Brook, Stony Brook, New York 11794, USA
    J Virol 73:3951-9. 1999
    ..Since beta3 integrins regulate vascular permeability and platelet function, these findings also correlate beta3 integrin usage with common elements of hantavirus pathogenesis...
  37. pmc Analysis of Rab GTPase-activating proteins indicates that Rab1a/b and Rab43 are important for herpes simplex virus 1 secondary envelopment
    Helen L Zenner
    Division of Virology, Department of Pathology, University of Cambridge, Cambridge CB2 1QP, UK
    J Virol 85:8012-21. 2011
    ....
  38. pmc The Ebola virus ribonucleoprotein complex: a novel VP30-L interaction identified
    A Groseth
    National Laboratory for Zoonotic Diseases and Special Pathogens, National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg R3E 3R2, Canada
    Virus Res 140:8-14. 2009
    ..The identification of these interactions allows a preliminary model of the EBOV RNP complex structure to be proposed, and may provide insight into filovirus transcriptional regulation...
  39. pmc Double-stranded RNA is produced by positive-strand RNA viruses and DNA viruses but not in detectable amounts by negative-strand RNA viruses
    Friedemann Weber
    Abteilung Virologie, Institut fur Medizinische Mikrobiologie und Hygiene, Universitat Freiburg, D 79008 Freiburg, Germany
    J Virol 80:5059-64. 2006
    ..Surprisingly, however, no dsRNA signals were detected for negative-strand RNA viruses. Thus, dsRNA is indeed a general feature of most virus groups, but negative-strand RNA viruses appear to be an exception to that rule...
  40. pmc NS1 protein of influenza A virus down-regulates apoptosis
    O P Zhirnov
    D I Ivanovsky Institute of Virology, Moscow 123098, Russia
    J Virol 76:1617-25. 2002
    ..As a consequence, delNS1 virus was also more lethal for chicken embryos than WT virus. In IFN-deficient Vero cells, however, apoptosis was delayed and developed with similar intensity after infection with both viruses...
  41. ncbi Non-muscle myosin IIA is a functional entry receptor for herpes simplex virus-1
    Jun Arii
    Division of Viral Infection, Department of Infectious Disease Control, International Research Center for Infectious Diseases, The Institute of Medical Science, The University of Tokyo, Minato ku, Tokyo 108 8639, Japan
    Nature 467:859-62. 2010
    ..The identification of NMHC-IIA as an HSV-1 entry receptor and the involvement of NM-IIA regulation in HSV-1 infection provide an insight into HSV-1 entry and identify new targets for antiviral drug development...
  42. pmc Measles viruses on throat swabs from measles patients use signaling lymphocytic activation molecule (CDw150) but not CD46 as a cellular receptor
    N Ono
    Department of Virology, Faculty of Medicine, Kyushu University, Fukuoka 812-8582, Japan
    J Virol 75:4399-401. 2001
    ..The viruses on throat swabs from nine patients with measles in Japan were titrated on Vero cells stably expressing human SLAM...
  43. pmc Reverse genetics with a full-length infectious cDNA of severe acute respiratory syndrome coronavirus
    Boyd Yount
    Department of Epidemiology and Microbiology, University of North Carolina, Chapel Hill, NC 27599 7435, USA
    Proc Natl Acad Sci U S A 100:12995-3000. 2003
    ....
  44. pmc Spontaneous mutation rate of measles virus: direct estimation based on mutations conferring monoclonal antibody resistance
    S J Schrag
    Respiratory and Enteric Viruses Branch, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30033, USA
    J Virol 73:51-4. 1999
    ..In the field, however, measles virus shows marked genetic stability. We briefly discuss the evolutionary implications of these results...
  45. pmc Incorporation of the green fluorescent protein into the herpes simplex virus type 1 capsid
    P Desai
    Virology Laboratories, Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Virol 72:7563-8. 1998
    ....
  46. pmc Human macrophage C-type lectin specific for galactose and N-acetylgalactosamine promotes filovirus entry
    Ayato Takada
    Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Tokyo 108 8639, Japan
    J Virol 78:2943-7. 2004
    ..We propose that filoviruses use different C-type lectins to gain cellular entry, depending on the cell type, and promote efficient viral replication...
  47. pmc Dengue virus type 1 nonstructural glycoprotein NS1 is secreted from mammalian cells as a soluble hexamer in a glycosylation-dependent fashion
    M Flamand
    Unité des Arbovirus et Virus des Fièvres Hémorragiques, Institut Pasteur, 75724 Paris Cedex 15, 91198 Gif sur Yvette Cedex, France
    J Virol 73:6104-10. 1999
    ..These results, together with observations related to the absence of secretion of NS1 from Den-infected insect cells, suggest that maturation and secretion of hexameric NS1 depend on the glycosylation status of the host cell...
  48. pmc Characterization of Ebola virus entry by using pseudotyped viruses: identification of receptor-deficient cell lines
    R J Wool-Lewis
    Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia 19104 6076, USA
    J Virol 72:3155-60. 1998
    ....
  49. pmc Rapid detection and quantification of RNA of Ebola and Marburg viruses, Lassa virus, Crimean-Congo hemorrhagic fever virus, Rift Valley fever virus, dengue virus, and yellow fever virus by real-time reverse transcription-PCR
    Christian Drosten
    Bernhard Nocht Institute of Tropical Medicine, Hamburg, Germany
    J Clin Microbiol 40:2323-30. 2002
    ..6 to 16 RNA copies per assay). The suitability of the assays was exemplified by detection and quantification of viral RNA in serum samples of VHF patients...
  50. pmc Role of kinesin-1 and cytoplasmic dynein in endoplasmic reticulum movement in VERO cells
    Marcin J Woźniak
    Faculty of Life Sciences, University of Manchester, The Michael Smith Building, Oxford Road, Manchester, UK M13 9PT
    J Cell Sci 122:1979-89. 2009
    ..We have analysed ER dynamics in living VERO cells and find that most ER tubule extension is driven by microtubule motors...
  51. pmc Herpes simplex virus-infected cell protein 0 blocks the silencing of viral DNA by dissociating histone deacetylases from the CoREST-REST complex
    Haidong Gu
    Marjorie B Kovler Viral Oncology Laboratories, University of Chicago, 910 East 58th Street, Chicago, IL 60637, USA
    Proc Natl Acad Sci U S A 104:17134-9. 2007
    ..1 pfu of virus per cell was 100-, 10-, and 10-fold higher, respectively, than those of R8501 mutant virus. In Vero cells, the yield of R8502 was identical with that of wild-type virus...
  52. pmc Infectious entry of West Nile virus occurs through a clathrin-mediated endocytic pathway
    J J H Chu
    Flavivirology Laboratory, Department of Microbiology, 5 Science Dr 2, National University of Singapore, Singapore 117597, Singapore
    J Virol 78:10543-55. 2004
    ..In situ hybridization of DNA probes specific for viral RNA demonstrated the trafficking of uncoated viral RNA genomes to the endoplasmic reticulum...
  53. pmc Heterocellular induction of interferon by negative-sense RNA viruses
    S Chen
    School of Biology, Centre for Biomolecular Sciences, BMS Building, North Haugh, University of St Andrews, St Andrews, Fife, KY16 9ST, UK
    Virology 407:247-55. 2010
    ..We show that only a limited number of infected cells are responsible for the production of IFN, and that this heterocellular production is a feature of the infecting virus as opposed to an intrinsic property of the cells...
  54. pmc The Lassa virus glycoprotein precursor GP-C is proteolytically processed by subtilase SKI-1/S1P
    O Lenz
    Institute of Virology, Philipps University, Robert Koch Strasse 17, 35037 Marburg, Germany
    Proc Natl Acad Sci U S A 98:12701-5. 2001
    ..To our knowledge, there have been no previous reports of this type of viral glycoprotein processing, one that may be an interesting target for antiviral therapy...
  55. pmc Cellular and viral requirements for rapid endocytic entry of herpes simplex virus
    Anthony V Nicola
    Medical Virology Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892 1888, USA
    J Virol 78:7508-17. 2004
    ..Thus, similar to entry via direct penetration at the cell surface, HSV entry into cells by wortmannin-sensitive endocytosis is efficient, involves rapid cellular uptake of viral particles, and requires gB, gD, and gH-gL...
  56. pmc Human membrane cofactor protein (CD46) acts as a cellular receptor for measles virus
    D Naniche
    CNRS ENS Lyon UMR 49, France
    J Virol 67:6025-32. 1993
    ..These results show that the human CD46 molecule serves as an MV receptor allowing virus-cell binding, fusion, and viral replication and open new perspectives in the study of MV pathogenesis...
  57. pmc A SAP30 complex inhibits IFN-beta expression in Rift Valley fever virus infected cells
    Nicolas Le May
    Unite de Genetique Moleculaire des Bunyavirus, Institut Pasteur, Paris, France
    PLoS Pathog 4:e13. 2008
    ..We discuss here the strategy developed by the highly pathogenic RVFV to evade the host antiviral response, affecting nuclear organization and IFN-beta promoter chromatin structure...
  58. pmc Influenza virus evades innate and adaptive immunity via the NS1 protein
    Ana Fernandez-Sesma
    Department of Microbiology, Mount Sinai School of Medicine, One Gustave L Levy Place, New York, NY 10029, USA
    J Virol 80:6295-304. 2006
    ..Our observations also support the potential use of NS1 mutant influenza viruses as live attenuated influenza virus vaccines...
  59. pmc Enhanced growth of influenza vaccine seed viruses in vero cells mediated by broadening the optimal pH range for virus membrane fusion
    Shin Murakami
    Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Tokyo, Japan
    J Virol 86:1405-10. 2012
    ..African green monkey Vero cells are recommended by the World Health Organization as a safe substrate for influenza vaccine production for human ..
  60. pmc Efficient multiplication of human metapneumovirus in Vero cells expressing the transmembrane serine protease TMPRSS2
    Yuta Shirogane
    Department of Virology, Faculty of Medicine, Kyushu University, Fukuoka, Japan
    J Virol 82:8942-6. 2008
    ..By generating Vero cells constitutively expressing the transmembrane serine protease TMPRSS2 and green fluorescent protein-expressing ..
  61. pmc Infectious cDNA clone of the epidemic west nile virus from New York City
    Pei Yong Shi
    Wadsworth Center, New York State Department of Health Department of Biomedical Sciences, University at Albany, State University of New York, Albany, New York 12201, USA
    J Virol 76:5847-56. 2002
    ..The stable infectious cDNA clone of the epidemic lineage I strain will provide a valuable experimental system to study the pathogenesis and replication of WNV...
  62. pmc Small rho GTPases and cholesterol biosynthetic pathway intermediates in African swine fever virus infection
    Jose I Quetglas
    Departamento de Biotecnologia Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria, INIA, Madrid, Spain
    J Virol 86:1758-67. 2012
    ....
  63. pmc Human SCARB2-mediated entry and endocytosis of EV71
    Yi Wen Lin
    National Institutes of Infectious Disease and Vaccinology, National Health Research Institutes, Taiwan, Republic of China
    PLoS ONE 7:e30507. 2012
    ..EV71 infection and subsequent inhibit the expression of viral capsid proteins in RD and 3T3-SCARB2 but not Vero cells. SiRNAs specific to clathrin or dynamin or chemical inhibitor of clathrin-mediated endocytosis were all capable ..
  64. pmc The host response to West Nile Virus infection limits viral spread through the activation of the interferon regulatory factor 3 pathway
    Brenda L Fredericksen
    Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, TX 75390 9048, USA
    J Virol 78:7737-47. 2004
    ..Although the activation of the IRF-3 pathway was not sufficient to block virus replication, our results suggest that IRF-3 target genes function to constrain WNV infection and limit cell-to-cell virus spread...
  65. pmc Inhibition of interferon-stimulated JAK-STAT signaling by a tick-borne flavivirus and identification of NS5 as an interferon antagonist
    Sonja M Best
    Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, NIAID, NIH, Hamilton, MT 59840, USA
    J Virol 79:12828-39. 2005
    ..Because NS5 may interfere with both innate and acquired immune responses to virus infection, this protein may have a significant role in viral pathogenesis...
  66. ncbi Quantitation of flaviviruses by fluorescent focus assay
    Anne F Payne
    The Arbovirus Laboratories, Wadsworth Center, New York State Department of Health, 5668 State Farm Rd, Slingerlands, NY 12159, USA
    J Virol Methods 134:183-9. 2006
    ..Louis encephalitis virus (SLEV), and Dengue virus (DENV) types 1-4. Vero cells were plated in 8-well chamber slides, and infected with 10-fold serial dilutions of virus...
  67. ncbi Individual and combined effects of ochratoxin A and citrinin on viability and DNA fragmentation in cultured Vero cells and on chromosome aberrations in mice bone marrow cells
    Amel Bouslimi
    Laboratory of Research on Biologically Compatible Compounds, Faculty of Dentistry, Rue Avicenne, 5019 Monastir, Tunisia
    Toxicology 251:1-7. 2008
    ..we have assessed their effects individually or combined on cell proliferation and DNA fragmentation in cultured Vero cells and in vivo by monitoring the induction of chromosome aberrations...
  68. pmc Phase I trial of intraperitoneal administration of an oncolytic measles virus strain engineered to express carcinoembryonic antigen for recurrent ovarian cancer
    Evanthia Galanis
    Division of Medical Oncology, Department of Molecular Medicine, Mayo Clinic, Gonda 10 141, 200 First Street Southwest, Rochester, MN 55905, USA
    Cancer Res 70:875-82. 2010
    ..Our findings indicate that i.p. administration of MV-CEA is well tolerated and results in dose-dependent biological activity in a cohort of heavily pretreated recurrent ovarian cancer patients...
  69. ncbi A single amino acid substitution in the central portion of the West Nile virus NS4B protein confers a highly attenuated phenotype in mice
    Jason A Wicker
    Department of Microbiology and Immunology, Sealy Center for Vaccine Development, Center for Biodefense and Emerging Infectious Diseases, and Institute for Human Infections and Immunity, University of Texas Medical Branch at Galveston, TX 77555 0609, USA
    Virology 349:245-53. 2006
    ....
  70. pmc Proteolysis of the Ebola virus glycoproteins enhances virus binding and infectivity
    Rachel L Kaletsky
    Department of Microbiology, University of Pennsylvania School of Medicine, 225 Johnson Pavilion, 3610 Hamilton Walk, Philadelphia, PA 19104 6076, USA
    J Virol 81:13378-84. 2007
    ..The influence of CatL in Ebola virus GP receptor binding should be useful in future studies characterizing the mechanism of Ebola virus entry...
  71. ncbi Dual therapy of ovarian cancer using measles viruses expressing carcinoembryonic antigen and sodium iodide symporter
    Kosei Hasegawa
    Molecular Medicine Program, Mayo Clinic College of Medicine, Rochester, Minnesota 55905, USA
    Clin Cancer Res 12:1868-75. 2006
    ..Because of the unfavorable replication kinetics of measles viruses expressing both CEA and NIS, we explored the feasibility of combining MV-CEA with MV-NIS for comprehensive virotherapy monitoring in ovarian cancer...
  72. ncbi Generation of murine monoclonal antibodies which cross-neutralize human enterovirus genogroup B isolates
    Hsuen Wen Chang
    Vaccine Research and Development Center, National Health Research Institutes, Zhunan Township, Miaoli County 350, Taiwan
    J Virol Methods 173:189-95. 2011
    A live enterovirus 71 (EV71) isolate designated, EV71/E59, with genotype B4 produced in Vero cells and purified over a sucrose gradient was used as the immunogen to generate EV71-specific murine monoclonal antibodies...
  73. pmc Vesicular stomatitis virus-based Ebola vaccines with improved cross-protective efficacy
    Andrea Marzi
    Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institute of Health, Hamilton, Montana 59840, USA
    J Infect Dis 204:S1066-74. 2011
    ..Our data demonstrate that cross-protection between the EBOV species can be achieved, although EBOV-GP alone cannot induce the required immune response...
  74. pmc Dynamin- and clathrin-dependent endocytosis in African swine fever virus entry
    Bruno Hernaez
    Departamento de Biotecnologia Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria, INIA, Autovia A6 km 7, 28040 Madrid, Spain
    J Virol 84:2100-9. 2010
    ..These results suggest a dynamin-dependent and clathrin-mediated endocytic pathway of ASFV entry for the cell types and viral strains analyzed...
  75. pmc A forward genetic strategy reveals destabilizing mutations in the Ebolavirus glycoprotein that alter its protease dependence during cell entry
    Anthony C Wong
    Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    J Virol 84:163-75. 2010
    ..Altogether, our findings suggest that CatB cleavage is required to facilitate the triggering of viral membrane fusion by destabilizing the prefusion conformation of EBOV GP...
  76. ncbi Analysis of filovirus entry into vero e6 cells, using inhibitors of endocytosis, endosomal acidification, structural integrity, and cathepsin (B and L) activity
    Anthony Sanchez
    Special Pathogens Branch, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
    J Infect Dis 196:S251-8. 2007
    ..These observations obtained using wild-type viruses confirm the results of studies utilizing recombinant systems and offer additional insights into filovirus entry...
  77. ncbi Efficacy, safety, and immunogenicity of a Vero-cell-culture-derived trivalent influenza vaccine: a multicentre, double-blind, randomised, placebo-controlled trial
    P Noel Barrett
    Global Research and Development, Baxter BioScience, Biomedical Research Centre, Orth Donau, Austria
    Lancet 377:751-9. 2011
    ..We investigated the safety, immunogenicity, and protective efficacy of a Vero-cell-culture-derived influenza vaccine, and assessed the correlation between vaccine efficacy and haemagglutination inhibition antibody titre...
  78. ncbi Antiviral activity of quercetin 7-rhamnoside against porcine epidemic diarrhea virus
    Hwa Jung Choi
    Natural Medicines Research Centre, Korea Research Institute of Bioscience and Biotechnology, Taejon 305 333, Republic of Korea
    Antiviral Res 81:77-81. 2009
    ....
  79. pmc Rinderpest virus blocks type I and type II interferon action: role of structural and nonstructural proteins
    Sambit K Nanda
    Institute for Animal Health, Ash Road, Pirbright, Surrey GU24 0NF, United Kingdom
    J Virol 80:7555-68. 2006
    ..Although a change in the distribution pattern of STAT2 was observed in virus-infected cells, STAT2 was not bound by any viral protein...
  80. ncbi The antiviral activity of sulfated polysaccharides against dengue virus is dependent on virus serotype and host cell
    L B Talarico
    Laboratorio de Virologia, Departamento de Quimica Biologica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
    Antiviral Res 66:103-10. 2005
    ..Both seaweed derivatives were selective inhibitors of DENV-2 multiplication in Vero cells with inhibitory concentration 50% (IC50) values around 1 microg/ml and selectivity indices > 1000...
  81. ncbi High immunogenic enterovirus 71 strain and its production using serum-free microcarrier Vero cell culture
    Chia Chyi Liu
    Institute of Biotechnology, Department of Life Science, National Tsing Hua University, Hsinchu, Taiwan
    Vaccine 25:19-24. 2007
    ..Therefore, serum-free microcarrier culture is a valuable process for developing inactivated EV71 vaccines...
  82. ncbi Contribution of the respiratory syncytial virus G glycoprotein and its secreted and membrane-bound forms to virus replication in vitro and in vivo
    M N Teng
    Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, 7 Center Dr. MSC 0720, Bethesda, Maryland 20892-0720, USA
    Virology 289:283-96. 2001
    ..In Vero cells, the DeltaG virus formed plaques and grew as efficiently as wild-type rRSV and mG...
  83. pmc The relationships between West Nile and Kunjin viruses
    J H Scherret
    Department of Microbiology and Parasitology, University of Queensland, Brisbane, Queensland 4072, Australia
    Emerg Infect Dis 7:697-705. 2001
    ..Our results suggest that KUN and WN viruses comprise a group of closely related viruses that can be differentiated into subgroups on the basis of genetic and antigenic analyses...
  84. pmc Ebola virus VP30-mediated transcription is regulated by RNA secondary structure formation
    Michael Weik
    Institut für Virologie der Philipps Universität Marburg, 35037 Marburg, Germany
    J Virol 76:8532-9. 2002
    ..Our results suggest that VP30 regulates a very early step of EBOV transcription, most likely by inhibiting pausing of the transcription complex at the RNA structure of the first transcription start site...
  85. pmc Processing of the Ebola virus glycoprotein by the proprotein convertase furin
    V E Volchkov
    Institut fur Virologie, Philipps Universitat Marburg, 35011 Marburg, Germany
    Proc Natl Acad Sci U S A 95:5762-7. 1998
    ..As a result of these observations, it should now be considered that proteolytic processing of GP may be an important determinant for the pathogenicity of Ebola virus...
  86. pmc West Nile virus inhibits the signal transduction pathway of alpha interferon
    Ju Tao Guo
    Institute for Cancer Research, Fox Chase Cancer Center, 333 Cottman Ave, Philadelphia, PA 19111, USA
    J Virol 79:1343-50. 2005
    ..Moreover, we demonstrated that the viral nonstructural proteins are responsible for this effect. Thus, our results provided an explanation for the observed resistance of WNV to IFN-alpha in cells of vertebrate origin...
  87. pmc A ferret model of canine distemper virus virulence and immunosuppression
    Veronika von Messling
    Molecular Medicine Program, Mayo Clinic, Rochester, Minnesota 55905, USA
    J Virol 77:12579-91. 2003
    ..Thus, virulence-inducing mutations have accumulated throughout the genome...
  88. ncbi Assessment of immunogenic potential of Vero adapted formalin inactivated vaccine derived from novel ECSA genotype of Chikungunya virus
    Mugdha Tiwari
    Defence Research and Development Establishment, Gwalior, M P, India
    Vaccine 27:2513-22. 2009
    ....
  89. ncbi Rescue and propagation of fully retargeted oncolytic measles viruses
    Takafumi Nakamura
    Nat Biotechnol 23:209-14. 2005
    ..These data provide an in vivo demonstration of antibody-directed tumor destruction by retargeted oncolytic viruses...
  90. ncbi High CD46 receptor density determines preferential killing of tumor cells by oncolytic measles virus
    Bambi D Anderson
    Molecular Medicine Program, Mayo Clinic Cancer Center, Mayo Clinic College of Medicine, 200 First Street SW, Rochester, MN 55905, USA
    Cancer Res 64:4919-26. 2004
    ..Discrimination between high and low CD46 receptor density provides a compelling basis for the oncolytic specificity of MV-Edm and establishes MV-Edm as a promising CD46-targeted cancer therapeutic agent...
  91. ncbi A mouse model for evaluation of prophylaxis and therapy of Ebola hemorrhagic fever
    M Bray
    Division of Virology, US Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland 21702 5011, USA
    J Infect Dis 178:651-61. 1998
    ..Mice injected peripherally with mouse-adapted or intraperitoneally with non-adapted EBO-Z virus resisted subsequent challenge with mouse-adapted virus...
  92. ncbi GP mRNA of Ebola virus is edited by the Ebola virus polymerase and by T7 and vaccinia virus polymerases
    V E Volchkov
    State Research Centre of Virology and Biotechnology Vector, Institute of Molecular Biology, Koltsovo, Novosibirsk Region, Russia
    Virology 214:421-30. 1995
    ..Our data indicate that the editing site in the Ebola virus glycoprotein gene is recognized not only by Ebola virus polymerase but also by DNA-dependent RNA polymerases of different origin...
  93. ncbi SLAM (CDw150) is a cellular receptor for measles virus
    H Tatsuo
    Department of Virology, Faculty of Medicine, Kyushu University, Fukuoka, Japan
    Nature 406:893-7. 2000
    ..The identification of SLAM as a receptor for measles virus opens the way to a better understanding of the pathogenesis of measles virus infection, especially the immunosuppression induced by measles virus...
  94. ncbi Optimization of microcarrier cell culture process for the inactivated enterovirus type 71 vaccine development
    Suh Chin Wu
    Department of Life Science, Institute of Biotechnology, National Tsing Hua University, Hsinchu, Taiwan
    Vaccine 22:3858-64. 2004
    ..In this paper, Vero cells were found to produce higher titers of EV71 than did MRC-5 and WI-38 cells...
  95. ncbi Isolation of Nipah virus from Malaysian Island flying-foxes
    Kaw Bing Chua
    Department of Medical Microbiology, Faculty of Medicine, University of Malaya, 50603 Lumpur, Kuala, Malaysia
    Microbes Infect 4:145-51. 2002
    ..urine and one from a partially eaten fruit swab) that caused Nipah virus-like syncytial cytopathic effect in Vero cells and stained strongly with Nipah- and Hendra-specific antibodies were isolated...
  96. pmc West nile virus capsid degradation of claudin proteins disrupts epithelial barrier function
    Guruprasad R Medigeshi
    Vaccine and Gene Therapy Institute, Oregon Health and Science University, Beaverton, Oregon 97006, USA
    J Virol 83:6125-34. 2009
    ..Since epithelial cells are frequent sites of WNV infection, these observations imply a potential mechanism for virus dissemination and extraneural pathogenesis...
  97. pmc Respiratory syncytial virus (RSV) SH and G proteins are not essential for viral replication in vitro: clinical evaluation and molecular characterization of a cold-passaged, attenuated RSV subgroup B mutant
    R A Karron
    Center for Immunization Research, Department of International Health, School of Hygiene and Public Health, The Johns Hopkins University, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 94:13961-6. 1997
    ..vaccine virus, designated respiratory syncytial virus (RSV) B1 cp-52/2B5 (cp-52), replicated efficiently in Vero cells, but was found to be overattenuated for RSV-seronegative infants and children...
  98. pmc Herpes simplex virus 1 ICP4 forms complexes with TFIID and mediator in virus-infected cells
    Jonathan T Lester
    Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, 514 Bridgeside Point II, 450 Technology Dr, Pittsburgh, PA 15219, USA
    J Virol 85:5733-44. 2011
    ..Taken together, the data suggest that ICP4 interacts with components of TFIID and Mediator in the context of viral infection, and this may explain the broad transactivation properties of ICP4...
  99. pmc A molecularly cloned Schwarz strain of measles virus vaccine induces strong immune responses in macaques and transgenic mice
    Chantal Combredet
    Unite des Virus Lents, CNRS URA 1930, Paris, France
    J Virol 77:11546-54. 2003
    ..The additional transcription units allow expressing heterologous antigens, thereby providing polyvalent vaccines based on an approved, safe, and efficient MV vaccine strain that is used worldwide...
  100. pmc Construction and characterization of recombinant flaviviruses bearing insertions between E and NS1 genes
    Myrna C Bonaldo
    Fundacao Oswaldo Cruz, Instituto Oswaldo Cruz, Laboratorio de Biologia Molecular, de Flavivírus, Rio de Janeiro, Fundacao Oswaldo Cruz, Avenida Brasil 4365, Manguinhos, Rio de Janeiro, RJ 21045 900, Brazil
    Virol J 4:115. 2007
    ....
  101. pmc West Nile virus growth is independent of autophagy activation
    Erica Beatman
    Department of Medicine, Division of Infectious Diseases, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA
    Virology 433:262-72. 2012
    ..These data show that WNV-induces autophagy but viral growth is independent of autophagy activation suggesting that WNV-specific interactions with autophagy have diverged from other flaviviruses...

Research Grants63

  1. Characterization of a novel rabbit herpes simplexvirus: a possible model for unde
    LING J JIN; Fiscal Year: 2010
    ..the skin of infected rabbits produced syncytia and cell lysis in cultured rabbit skin and kidney cells, and in Vero cells. PCR using degenerative primers to conserved herpesvirus genes was used to amplify sequences from purified viral ..
  2. Developing a Paramyxovirus-based H5N1 Vaccine
    Biao He; Fiscal Year: 2012
    ..PIV5 can be produced in high titers (up to 8x108 plaque forming units (PFU)/ml) in many cells including Vero cells, which are WHO approved for vaccine production. PIV5 can infect human cell lines as well as primary human cells...
  3. A Novel Approach for Mycobacterium Tuberculosis Vaccine Development
    Biao He; Fiscal Year: 2013
    ..Second, it can be produced in high titers in many cells including Vero cells that have been approved for vaccine production. Third, PIV5 can infect human cell lines and primary human cells...
  4. Newcastle Disease Virus Vectored Vaccines for Norovirus Infection
    Siba K Samal; Fiscal Year: 2013
    ..VLPs expressed by NDV will also be purified from infected allantoic fluids and Vero cells. Additionally, NDV expressing murine norovirus capsid protein and its purified VLPs from infected allantoic ..
  5. Human Rabies Virus Vaccine Development
    James P McGettigan; Fiscal Year: 2012
    ..of the M gene-deleted RV using protocols developed during Phase I to recover and propagate M gene-deleted RVs on Vero cells (a pharmaceutically acceptable cell substrate)...
  6. Development of a Novel, Chimeric Chikungunya Vaccine
    Richard Kinney; Fiscal Year: 2009
    ..generate and optimize chimeric cDNA clones to produce candidate vaccine strains that replicate efficiently in Vero cells, and are highly attenuated yet rapidly immunogenic and efficacious in preventing CHIK disease...
  7. RNA Interference for the Treatment of Ebola and Marburg Hemorrhagic Fever
    Ian MacLachlan; Fiscal Year: 2013
    ..siRNAs will be identified by testing their ability to inhibit replication of infectious EBOV and MARV in Vero cells and also by using a nonviral plasmid-based expression system...
  8. Chimeric Alphavirus Vaccines for Chikungunya
    Scott C Weaver; Fiscal Year: 2010
    ..and structural protein genes from CHIKV, we will optimize vaccine candidates that replicate efficiently in Vero cells, and are highly attenuated yet rapidly immunogenic and efficacious in preventing viremia and disease...
  9. Novel Vaccine Technology for Chikungunya
    Scott C Weaver; Fiscal Year: 2013
    ..3. Optimize the vaccine candidate for production in Vero cells. 4. Develop and characterize GMP master virus seed and working virus seed stocks. 5...
  10. Preclinical characterization of a multivalent killed Filovirus/Rabies vaccine
    MATTHIAS JOHANNES SCHNELL; Fiscal Year: 2013
    ..For this approach, we will adapt the RABV recovery system to Vero cells in a GMP facility, establish a Vero cell bank suitable for production of the vaccine as well as viral seed ..
  11. Discovery and Optimization of Novel Integrase Inhibitors as Anti-HIV Agents
    John K Buolamwini; Fiscal Year: 2010
    ..Toxicity of compounds will be tested using uninfected PBMCs as well as CEM leukemia and Vero cells to assess therapeutic index...
  12. Identification and Role of Type IV Effector Proteins in Coxiella burnetii
    JAMES EVANS SAMUEL; Fiscal Year: 2010
    ..burnetii. 3) Establish effector function for T4SS substrates. ORFs will be ectopically expressed in Vero cells to localize specific interactions. Antibody will be generated to localize distribution when expressed by C...
  13. A SARS-CoV Spike Protein Vaccine
    B Sim; Fiscal Year: 2005
    ..Anti-sera will be assessed for antibodies to spike protein by ELISA and capacity to neutralize virus invasion of Vero cells in vitro...
  14. A high-growth PR8 virus for pandemic vaccine production in ST6-Vero cells
    Yoshihiro Kawaoka; Fiscal Year: 2012
    DESCRIPTION (provided by applicant): A high-growth PR8 virus for pandemic vaccine production in ST6-Vero cells. In 2009, the traditional means of vaccine manufacturing for the H1N1 influenza pandemic failed to yield sufficient doses ..
  15. GATING AND TRANSLOCATION IN THE DIPHTHERIA TOXIN CHANNEL
    Bruce Kagan; Fiscal Year: 1992
    ..Patch clamp experiments will attempt to record DT channels in Vero cells as they cross the cell membrane...
  16. HTS assay and configuration for inhibitors of the UDP-Mur formation pathway
    Michael McNeil; Fiscal Year: 2009
    ..We will also follow up by performing initial toxicity testing against Vero cells. Further development will focus on development of hits to drugs against tuberculosis as this is our lab's ..
  17. New generation of efficient vaccines against encephalitogenic alphaviruses
    ILYA V FROLOV; Fiscal Year: 2012
    ..will cause an abortive infection in vivo, but they will sustain the ability for replication to high titers in Vero cells. iv) The genomes of the designed viruses will contain additional, extensive modifications, which make these ..
  18. ACTIN BASED MOTILITY BY RICKETTSIA RICKETTSII
    ROBERT HEINZEN; Fiscal Year: 2002
    ..Transfected Vero cells producing specific domains of rOmpA or rOmpB, and infected with R...
  19. GLYCOLIPIDS AND HEMOLYTIC UREMIC SYNDROME
    David Haslam; Fiscal Year: 2002
    ..In preliminary studies VT-susceptible Vero cells were transfected with the cDNA encoding Forssman synthetase (FS)...
  20. CHIMERIC YF/DEN2 AS A VACCINE CANDIDATE FOR DENGUE VIRUS
    Farshad Guirakhoo; Fiscal Year: 1999
    ..Viruses were recovered upon transfection of Vero cells with mRNA and amplified twice in these cells to yield a titer of 6.3 log(10p)fu/ml...
  21. Ab Alphavirus Replicon Vaccine against Cytomegalovirus
    Robert Esch; Fiscal Year: 2005
    ..Purified RNA transcripts produced from the replicon and helper plasmids will be electroporated into certified Vero cells using various amounts and electroporation parameters to identify the optimal conditions for transfection ..
  22. Microcarrier culture system for human influenza vaccine
    WILLIAM HILLEGAS; Fiscal Year: 2004
    ..integrate critical cell culture and viral growth parameters for optimal production of human influenza virus in Vero cells grown in Hillex microcarrier-suspension culture. Specific Aim II...
  23. Recombinant Human Serum Albumin for Use in Cell Culture
    Ning Huang; Fiscal Year: 2010
    ..extend the studies in phase I to test the effect of rHSA on cell growth and productivity of hybridoma, CHO and Vero cells in larger scale cell culture with commercial media...
  24. ACTIVATION OF BACTERIAL TOXINS IN SUDDEN INFANT DEATH
    James Lindsay; Fiscal Year: 1991
    ..of different ages by examining changes in time to death, alterations to ileal tissue in vivo, and alterations to Vero cells in vitro. 3. To examine the incidence of C...
  25. GOSSYPOL DERIVATIVES AS COVALENT CARRIERS OF ANTIVIRALS
    Robert Royer; Fiscal Year: 1991
    ..In vitro studies of these new drugs will be carried out with HIV, using human T cells; with HSV-II, using Vero cells; and with influenza virus, using MDCK cells...
  26. CELLULAR IMMUNITY IN SICK AND MALNOURISHED INFANTS
    E Stiehm; Fiscal Year: 1991
    ..EVR is measured by a quantitative plaque reduction assay utilizing herpes simplex virus type I in Vero cells as well as assays of viral release into supernatants from infected CEM cell supernatants...
  27. CONTROL OF HIV-1 AND HSV-1 INFECTION WITH PLANT LIGNANS
    RU CHIH HUANG; Fiscal Year: 2000
    ..4 and / or related plant lignans can suppress wild type HSV-1 and recombinant HSV ICP4LACZ in African Green Monkey (VERO) cells,(3) to study the effect of plant lignan in controlling HSV 1 in infected Hu-PBL-SCID mice. ..
  28. HIV-LIKE PARTICLES: PRODUCTION AND PURIFICATION SCALE-UP
    STEVEN DISCIULLO; Fiscal Year: 1993
    ..HIV-like particles will be expressed in large-scale microcarrier cultures of Vero cells infected with the recombinant virus...
  29. Novel Compounds for Improved Treatment of Tuberculosis
    ALBERT OWENS; Fiscal Year: 2003
    ..MICs) against a panel of mycobacteria in vitro; 3) determine in vitro cytotoxicities (lC50s) against Vero cells; 4) determine maximum tolerated dose (MTD) and dose limiting toxicity in mice for compounds with selectivity ..
  30. Animal models to design & evaluate improved VZV vaccines
    WAYNE GRAY; Fiscal Year: 2007
    ..The study may also provide support for use of the VZV vaccine as a recombinant vector for immunization against other infectious agents, particularly human immunodeficiency virus (HIV). ..
  31. LIVE, ATTENUATED, RECOMBINANT VACCINE - WEST NILE VIRUS
    Thomas Monath; Fiscal Year: 2002
    ..abstract_text> ..
  32. Novel inhibitors of the replication of poxviruses
    Johan Neyts; Fiscal Year: 2004
    ..The final goal of this application is to advance a compound for the treatment of (systemic or cutaneous) poxvirus infections to the initial stages of development, i.e., selecting an IND candidate. ..
  33. MOLECULAR GENETICS OF HSV-1 CAPSIDS
    Prashant Desai; Fiscal Year: 2007
    ..Determine the location of the largest tegument protein, VPI/2, in the capsid shell. [unreadable] [unreadable]..
  34. DEVELOPMENT OF LIGAND ASSISTED ASYMMETRIC SYNTHESES
    Arun Ghosh; Fiscal Year: 2001
    ..Besides the broad range of scope and generality, this line of research will provide excellent opportunities to teach and train graduate and undergraduate students in the laboratory. ..
  35. Self-limiting, and Dominant-negative Herpes Simplex Virus Recombinants
    Feng Yao; Fiscal Year: 2007
    ..Specific Aim 4 will focus on investigating the influence of de novo expression of B7-1 and B7-2 by HSV-1 replication-defective vaccine virus on the augmentation of host immunity against HSV infection. [unreadable] [unreadable]..
  36. Development of an VEE Replicon Vaccine Against Smallpox
    Kurt Kamrud; Fiscal Year: 2005
    ....
  37. DESIGN AND SYNTHESIS OF NONPEPTIDE PROTEASE INHIBITORS
    Arun K Ghosh; Fiscal Year: 2010
    ..This research integrates organic synthesis, protein-ligand x-ray crystallography, molecular modeling and in-depth virus and cell-biological studies to design the next generation of HIV-1 protease inhibitors. ..
  38. Anti-Viral Gene Delivery in the Nervous System
    Daniel Carr; Fiscal Year: 2005
    ..abstract_text> ..
  39. Maturation functions of the HSV-1 tegument
    Prashant Desai; Fiscal Year: 2010
    ..translocation, Golgi stabilization, Golgi trafficking, virion incorporation and protein-protein interactions This analysis will identify domains of the proteins that have the potential to be used as targets for antiviral intervention ..
  40. Oral-Pharyngeal HHV-8 Infection
    Jeffrey Vieira; Fiscal Year: 2004
    ..abstract_text> ..
  41. NEUROIMMUNOLOGY OF AED IN OCULAR HSV-1 INFECTION
    Daniel Carr; Fiscal Year: 2001
    ..abstract_text> ..
  42. HOST COMPETENCE FACTORS AFFECT ARBOVIRUS TRANSMISSION
    William Reisen; Fiscal Year: 2003
    ..Studies will assess how alternating sequential infections between vertebrate and vector species that are part of the natural transmission cycle may alter virus genetics and fitness. ..
  43. ARBOVIRUS PERSISTENCE IN SEASONAL WETLANDS
    William Reisen; Fiscal Year: 2002
    ..Virus ecology at wetlands in the Coachella Valley of southeastern California with consistent enzootic activity will be compared with wetlands in the southern San Joaquin Valley where virus activity currently is intermittent. ..
  44. Herpes Simplex Virus Capsid Assembly and DNA Packaging
    FREDERICK L HOMA; Fiscal Year: 2010
    ..These studies aim to elucidate the mechanism underlying HSV DNA cleavage and packaging and may suggest novel targets for the development of antivirals. ..
  45. Population dynamics of a bird-borne arbovirus
    Charles Brown; Fiscal Year: 2007
    ..e., colony sites) can be reliably identified and ecological hypotheses tested. ..
  46. The Role of CXCL9 in Genital HSV-2 Infection
    Daniel Carr; Fiscal Year: 2009
    ..g., vaccine or gene transfer) to reduce the incidence of infection or reduce the capacity of latent virus to reactivate. ..
  47. Spontaneous Lytic Replication of KSHV
    Jeffrey Vieira; Fiscal Year: 2008
    ..2. Analyze the X219 genome to identify genetic differences compared to the parental virus. 3. Determine the genotype of X219 responsible for the phenotype of spontaneous lytic replication. ..
  48. Transmission and Acquisition of KSHV
    Jeffrey Vieira; Fiscal Year: 2008
    ..2. To investigate the transmission of KSHV across the oral mucosa using in vitro organ culture models. 3. To determine the cells of oral mucosa organ cultures that are sites of primary KSHV infection. ..
  49. Membrane Organization in Cell Signaling and Adhesion
    Tione Buranda; Fiscal Year: 2008
    ..Completion of the proposed work will yield a better mechanistic grasp of the dynamics of membrane organization as a factor in signaling and cell adhesion. ..
  50. Biology of Animal Nuclear Mononegaviruses
    JUAN DE LA TORRE; Fiscal Year: 2008
    ..abstract_text> ..
  51. Innate Corneal Cell Immunity to Virus Infection
    Jerry Taylor; Fiscal Year: 2004
    ..This knowledge may be utilized to enhance this innate antiviral response and thereby limit initial and recurrent viral infections in the eye. ..
  52. VIRUS SPECIFIC FACTORS OF HSV TO CORNEAL DISEASE
    CURTIS BRANDT; Fiscal Year: 2008
    ..g. isoform A is involved with function B). These studies will add to our knowledge of the role of the US1 protein in ocular HSV infections and may lead to improved therapies. ..
  53. Immunobiology of Influenza Virus Infection: Approaches for an Emerging Zoonotic D
    Ralph Tripp; Fiscal Year: 2007
    ..abstract_text> ..
  54. HERPES SIMPLEX VIRUS TERMINASES
    Joel Baines; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  55. Structure-Based Design of Nipah Virus Entry Inhibitors
    RICHARD PLEMPER; Fiscal Year: 2006
    ..These will be characterized for their activity against live Nipah (aim 3). ..
  56. The role of IL-2 in promoting CD8 memory T cell responsiveness
    Matthew Williams; Fiscal Year: 2008
    ..In this endeavor, we will explore the role of the growth factor interleukin-2 in enhancing the ability of memory cells to respond to a second encounter with a pathogen. [unreadable] [unreadable] [unreadable] [unreadable]..
  57. ASSEMBLY AND FUNCTION OF THE HERPES SIMPLEX ENVELOPE
    Duncan Wilson; Fiscal Year: 2005
    ..Information obtained from this study will help in the design of agents able to interfere with HSV envelopment, thus reducing the virulence of this serious human pathogen. ..
  58. REVERSE GENETICS OF ARENAVIRUSES
    JUAN DE LA TORRE; Fiscal Year: 2007
    ..We will use biochemical, genetics and functional assays to assess the relevance in Arenavirus biology of candidates initially identified by TAP/MS. ..
  59. ICP0-induced Cellular Factors Promote HSV-1 Replication
    RYAN BRINGHURST; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  60. Influenza virus assembly and virulence
    Andrew Pekosz; Fiscal Year: 2007
    ..abstract_text> ..
  61. Small Molecule Alanine Racemase Inhibitors as Novel Therapeutics for Tuberculosis
    Karen Anthony; Fiscal Year: 2006
    ..About 50 million people are infected with TB bacteria that are not susceptible to available antibiotics. The goal of this research is to develop new antibiotics to treat drug-resistant TB. [unreadable] [unreadable] [unreadable]..
  62. MINORITY PREDOCTORAL FELLOWSHIP PROGRAM
    Deveroux Ferguson; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  63. A High-Throughput Screen to Identify Small Molecule Compounds That Augment
    Brent Passer; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..