vero cells

Summary

Summary: A CELL LINE derived from the kidney of the African green (vervet) monkey, (CERCOPITHECUS AETHIOPS) used primarily in virus replication studies and plaque assays.

Top Publications

  1. ncbi Epidemiologic and virologic investigation of hand, foot, and mouth disease, southern Vietnam, 2005
    Phan Van Tu
    Pasteur Institute, Ho Chi Minh City, Vietnam
    Emerg Infect Dis 13:1733-41. 2007
  2. ncbi Roles for endocytosis and low pH in herpes simplex virus entry into HeLa and Chinese hamster ovary cells
    Anthony V Nicola
    Medical Virology Section, Laboratory of Clinical Investigation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Virol 77:5324-32. 2003
  3. ncbi Herpes simplex virus-1 entry into cells mediated by a novel member of the TNF/NGF receptor family
    R I Montgomery
    Northwestern University Medical School, Department of Microbiology Immunology, Chicago, Illinois 60611, USA
    Cell 87:427-36. 1996
  4. ncbi Endosomal proteolysis of the Ebola virus glycoprotein is necessary for infection
    Kartik Chandran
    Department of Medicine, Brigham and Women s Hospital and Harvard Medical School, Boston, MA 02115, USA
    Science 308:1643-5. 2005
  5. ncbi Cellular and viral requirements for rapid endocytic entry of herpes simplex virus
    Anthony V Nicola
    Medical Virology Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892 1888, USA
    J Virol 78:7508-17. 2004
  6. ncbi Assembly and maturation of the flavivirus Kunjin virus appear to occur in the rough endoplasmic reticulum and along the secretory pathway, respectively
    J M MacKenzie
    Clinical Medical Virology Centre, University of Queensland, St Lucia, Queensland 4072, Australia
    J Virol 75:10787-99. 2001
  7. ncbi Persistent infection with ebola virus under conditions of partial immunity
    Manisha Gupta
    Special Pathogens Branch, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    J Virol 78:958-67. 2004
  8. ncbi Rab9 GTPase is required for replication of human immunodeficiency virus type 1, filoviruses, and measles virus
    James L Murray
    National Center for HIV, STD, and TB Prevention, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    J Virol 79:11742-51. 2005
  9. ncbi The endoplasmic reticulum provides the membrane platform for biogenesis of the flavivirus replication complex
    Leah K Gillespie
    Department of Microbiology, La Trobe University, Bundoora, Melbourne 3086, Australia
    J Virol 84:10438-47. 2010
  10. ncbi Continuous cell lines as a production system for influenza vaccines
    Yvonne Genzel
    Max Planck Institute for Dynamics of Complex Technical Systems, Bioprocess Engineering Magdeburg, Sandtorstr 1, Magdeburg 39106, Germany
    Expert Rev Vaccines 8:1681-92. 2009

Research Grants

  1. A SARS-CoV Spike Protein Vaccine
    B Sim; Fiscal Year: 2005
  2. Developing a Paramyxovirus-based H5N1 Vaccine
    Biao He; Fiscal Year: 2010
  3. Developing a Paramyxovirus-based H5N1 Vaccine
    Biao He; Fiscal Year: 2009
  4. CHARACTERIZATION OF HCMV UL84
    Gregory Pari; Fiscal Year: 2009
  5. ACTIN BASED MOTILITY BY RICKETTSIA RICKETTSII
    ROBERT HEINZEN; Fiscal Year: 2002
  6. GLYCOLIPIDS AND HEMOLYTIC UREMIC SYNDROME
    David Haslam; Fiscal Year: 2002
  7. STUDIES ON DIPHTHERIA TOXIN AND ITS RECEPTOR
    Leon Eidels; Fiscal Year: 1990
  8. CYTOMEGALOVIRUS DNA REPLICATION AND RECOMBINATION
    Edward Mocarski; Fiscal Year: 1993
  9. Ab Alphavirus Replicon Vaccine against Cytomegalovirus
    Robert Esch; Fiscal Year: 2005
  10. DIPHTHERIA TOXIN AND ITS RECEPTOR
    Leon Eidels; Fiscal Year: 1993

Detail Information

Publications299 found, 100 shown here

  1. ncbi Epidemiologic and virologic investigation of hand, foot, and mouth disease, southern Vietnam, 2005
    Phan Van Tu
    Pasteur Institute, Ho Chi Minh City, Vietnam
    Emerg Infect Dis 13:1733-41. 2007
    ..In the second half of the year, viruses belonging to subgenogroup C5 predominated during a period of higher HEV71 activity...
  2. ncbi Roles for endocytosis and low pH in herpes simplex virus entry into HeLa and Chinese hamster ovary cells
    Anthony V Nicola
    Medical Virology Section, Laboratory of Clinical Investigation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Virol 77:5324-32. 2003
    Herpes simplex virus (HSV) infection of many cultured cells, e.g., Vero cells, can be initiated by receptor binding and pH-neutral fusion with the cell surface...
  3. ncbi Herpes simplex virus-1 entry into cells mediated by a novel member of the TNF/NGF receptor family
    R I Montgomery
    Northwestern University Medical School, Department of Microbiology Immunology, Chicago, Illinois 60611, USA
    Cell 87:427-36. 1996
    ..The contribution of HVEM to HSV entry into human cells was demonstrable in activated T cells. HVEM, the first identified mediator of HSV entry, is a new member of the TNF/NGF receptor family...
  4. ncbi Endosomal proteolysis of the Ebola virus glycoprotein is necessary for infection
    Kartik Chandran
    Department of Medicine, Brigham and Women s Hospital and Harvard Medical School, Boston, MA 02115, USA
    Science 308:1643-5. 2005
    ..CatB and CatB/CatL inhibitors diminish the multiplication of infectious EboV-Zaire in cultured cells and may merit investigation as anti-EboV drugs...
  5. ncbi Cellular and viral requirements for rapid endocytic entry of herpes simplex virus
    Anthony V Nicola
    Medical Virology Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892 1888, USA
    J Virol 78:7508-17. 2004
    ..Thus, similar to entry via direct penetration at the cell surface, HSV entry into cells by wortmannin-sensitive endocytosis is efficient, involves rapid cellular uptake of viral particles, and requires gB, gD, and gH-gL...
  6. ncbi Assembly and maturation of the flavivirus Kunjin virus appear to occur in the rough endoplasmic reticulum and along the secretory pathway, respectively
    J M MacKenzie
    Clinical Medical Virology Centre, University of Queensland, St Lucia, Queensland 4072, Australia
    J Virol 75:10787-99. 2001
    ..The results suggest that the assembly of virions occurs within the lumen of the RER and that subsequent maturation occurs via the secretory pathway...
  7. ncbi Persistent infection with ebola virus under conditions of partial immunity
    Manisha Gupta
    Special Pathogens Branch, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    J Virol 78:958-67. 2004
    ....
  8. ncbi Rab9 GTPase is required for replication of human immunodeficiency virus type 1, filoviruses, and measles virus
    James L Murray
    National Center for HIV, STD, and TB Prevention, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    J Virol 79:11742-51. 2005
    ..These results suggest that Rab9 is an important cellular target for inhibiting diverse viruses and help to define a late-endosome-to-plasma-membrane vesicular transport pathway important in viral assembly...
  9. ncbi The endoplasmic reticulum provides the membrane platform for biogenesis of the flavivirus replication complex
    Leah K Gillespie
    Department of Microbiology, La Trobe University, Bundoora, Melbourne 3086, Australia
    J Virol 84:10438-47. 2010
    ..In addition, we provide data that indicate that a majority of the viral RNA species housed within the RC is in a double-stranded RNA (dsRNA) form...
  10. ncbi Continuous cell lines as a production system for influenza vaccines
    Yvonne Genzel
    Max Planck Institute for Dynamics of Complex Technical Systems, Bioprocess Engineering Magdeburg, Sandtorstr 1, Magdeburg 39106, Germany
    Expert Rev Vaccines 8:1681-92. 2009
    ..These encouraging developments for cell culture-based influenza virus production are summarized and an overview on potential cell substrates is given...
  11. ncbi The NS5 protein of the virulent West Nile virus NY99 strain is a potent antagonist of type I interferon-mediated JAK-STAT signaling
    Maudry Laurent-Rolle
    Laboratory of Virology, Rocky Mountain Laboratories, 903 S Fourth St, Hamilton, MT 59840, USA
    J Virol 84:3503-15. 2010
    ..Thus, a naturally occurring mutation is associated with the function of NS5 in IFN antagonism and may influence virulence of WNV field isolates...
  12. ncbi Quantitation of flaviviruses by fluorescent focus assay
    Anne F Payne
    The Arbovirus Laboratories, Wadsworth Center, New York State Department of Health, 5668 State Farm Rd, Slingerlands, NY 12159, USA
    J Virol Methods 134:183-9. 2006
    ..Louis encephalitis virus (SLEV), and Dengue virus (DENV) types 1-4. Vero cells were plated in 8-well chamber slides, and infected with 10-fold serial dilutions of virus...
  13. ncbi Severe acute respiratory syndrome coronavirus evades antiviral signaling: role of nsp1 and rational design of an attenuated strain
    Marc G Wathelet
    Department of Molecular and Cellular Physiology, University of Cincinnati College of Medicine, 231 Albert Sabin Way, Cincinnati, OH 45267 0576, USA
    J Virol 81:11620-33. 2007
    ..However, mutant virus replication was strongly attenuated in cells with an intact IFN response. Thus, nsp1 is likely a virulence factor that contributes to pathogenicity by favoring SARS-CoV replication...
  14. ncbi Temporal analysis of Coxiella burnetii morphological differentiation
    Sherry A Coleman
    Coxiella Pathogenesis Unit, NIAID NIH, Rocky Mountain Laboratories, 903 S 4th Street, Hamilton, MT 59840, USA
    J Bacteriol 186:7344-52. 2004
    ..We developed a model to study C. burnetii morphogenesis that uses Vero cells synchronously infected with homogeneous SCV (Nine Mile strain in phase II) harvested from aged infected cell ..
  15. ncbi Phenotype of a herpes simplex virus type 1 mutant that fails to express immediate-early regulatory protein ICP0
    Roger D Everett
    MRC Virology Unit, Institute of Virology, Glasgow G11 5JR, Scotland, United Kingdom
    J Virol 78:1763-74. 2004
    ....
  16. ncbi Image-guided radiovirotherapy for multiple myeloma using a recombinant measles virus expressing the thyroidal sodium iodide symporter
    David Dingli
    Molecular Medicine Program, Mayo Clinic and Foundation, Rochester, MN 55905, USA
    Blood 103:1641-6. 2004
    ..Testing in other radiosensitive cancers is warranted...
  17. ncbi Glycoprotein D receptor-dependent, low-pH-independent endocytic entry of herpes simplex virus type 1
    Richard S B Milne
    Department of Microbiology, University of Pennsylvania, School of Dental Medicine, 215 Levy Building, 240 South 40th Street, Philadelphia, PA 19104 6002, USA
    J Virol 79:6655-63. 2005
    ..pathways have been described: direct fusion between the virion envelope and the plasma membrane, as seen on Vero cells, and low-pH-dependent endocytosis, as seen on CHO nectin-1 and HeLa cells...
  18. ncbi Assembly and budding of Ebolavirus
    Takeshi Noda
    Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan
    PLoS Pathog 2:e99. 2006
    ..These data form a foundation for the identification and development of potential antiviral agents to combat the devastating disease caused by this virus...
  19. ncbi Crimean-Congo hemorrhagic fever virus delays activation of the innate immune response
    Ida Andersson
    KCB Swedish Institute for Infectious Disease Control, Solna, Sweden
    J Med Virol 80:1397-404. 2008
    ..In summary, the results suggest the presence of a virulence factor encoded by CCHFV that delays the host defence in order to allow rapid viral spread in the host...
  20. ncbi Recovery of infectious Ebola virus from complementary DNA: RNA editing of the GP gene and viral cytotoxicity
    V E Volchkov
    Institut fur Virologie, Philipps Universitat, Robert Koch Strasse 17, 35037 Marburg, Germany
    Science 291:1965-9. 2001
    ..The mutant was significantly more cytotoxic than wild-type virus, indicating that cytotoxicity caused by GP is down-regulated by the virus through transcriptional RNA editing and expression of sGP...
  21. ncbi A rapid and quantitative assay for measuring antibody-mediated neutralization of West Nile virus infection
    Theodore C Pierson
    Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Virology 346:53-65. 2006
    ..WNV RVPs are capable of only a single round of infection, can be used under BSL-2 conditions, and offer a rapid and quantitative approach for detecting virus entry and its inhibition by neutralizing antibody...
  22. ncbi Microtubule-mediated transport of incoming herpes simplex virus 1 capsids to the nucleus
    B Sodeik
    Yale University School of Medicine, Department of Cell Biology, New Haven, Connecticut 06520 8002, USA
    J Cell Biol 136:1007-21. 1997
    ..presence and absence of microtubule depolymerizing agents, it was shown that the cytosolic capsid transport in Vero cells was mediated by microtubules...
  23. ncbi Human membrane cofactor protein (CD46) acts as a cellular receptor for measles virus
    D Naniche
    CNRS ENS Lyon UMR 49, France
    J Virol 67:6025-32. 1993
    ..These results show that the human CD46 molecule serves as an MV receptor allowing virus-cell binding, fusion, and viral replication and open new perspectives in the study of MV pathogenesis...
  24. ncbi Interaction of dengue virus envelope protein with endoplasmic reticulum-resident chaperones facilitates dengue virus production
    Thawornchai Limjindaporn
    Department of Anatomy, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand
    Biochem Biophys Res Commun 379:196-200. 2009
    ..These results indicate that the interaction of these three chaperones with DENV E plays an important role in virion production, likely facilitating proper folding and assembly of dengue proteins...
  25. ncbi Pre-clinical development of cell culture (Vero)-derived H5N1 pandemic vaccines
    M Keith Howard
    Baxter AG, Biomedical Research Center, Uferstrasse 15, A 2304 Orth Donau, Austria
    Biol Chem 389:569-77. 2008
    ..These data indicate that cell culture-grown, whole virus vaccines, based on the wild-type virus, allow the rapid high-yield production of a candidate pandemic vaccine...
  26. ncbi Antiviral activities of extracts from Hong Kong seaweeds
    Hui Wang
    Medical College, Jinan University, Guangzhou 510632, China
    J Zhejiang Univ Sci B 9:969-76. 2008
    ..HI-3, a compound fractionated from this hot water extract, showed a dose-dependent anti-HSV activity in flow cytometric analysis and plaque reduction assay...
  27. ncbi Efficacy, safety, and immunogenicity of a Vero-cell-culture-derived trivalent influenza vaccine: a multicentre, double-blind, randomised, placebo-controlled trial
    P Noel Barrett
    Global Research and Development, Baxter BioScience, Biomedical Research Centre, Orth Donau, Austria
    Lancet 377:751-9. 2011
    ..We investigated the safety, immunogenicity, and protective efficacy of a Vero-cell-culture-derived influenza vaccine, and assessed the correlation between vaccine efficacy and haemagglutination inhibition antibody titre...
  28. ncbi The hemagglutinin-neuraminidase protein of Newcastle disease virus determines tropism and virulence
    Zhuhui Huang
    Virginia-Maryland Regional College of Veterinary Medicine, University of Maryland, College Park, Maryland 20742, USA
    J Virol 78:4176-84. 2004
    ..These results are consistent with the hypothesis that the virulence of NDV is multigenic and that the cleavability of F protein alone does not determine the virulence of a strain...
  29. ncbi Anti-herpes simplex virus activity of sulfated galactans from the red seaweeds Gymnogongrus griffithsiae and Cryptonemia crenulata
    Laura B Talarico
    , , Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, , 1428 Buenos Aires, Argentina
    Int J Biol Macromol 34:63-71. 2004
    ..5-5.6 microg/ml, as determined in a virus plaque reduction assay in Vero cells. The galactans lacked cytotoxic effects and showed a broad spectrum of antiviral activity against HSV-1 and HSV-..
  30. ncbi One time intranasal vaccination with a modified vaccinia Tiantan strain MVTT(ZCI) protects animals against pathogenic viral challenge
    Wenbo Yu
    AIDS Center and Modern Virology Research Center, State Key Laboratory of Virology, College of Life Sciences, Wuhan University, PR China
    Vaccine 28:2088-96. 2010
    ..This study established proof-of-concept that the attenuated replicating MVTT(ZCI) may serve as a safe noninvasive smallpox vaccine candidate...
  31. ncbi Nuclear localization of Japanese encephalitis virus core protein enhances viral replication
    Yoshio Mori
    Research Center for Emerging Infectious Diseases, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamada-oka, Suita, Osaka 565-0871, Japan
    J Virol 79:3448-58. 2005
    ..M4243 virus exhibited RNA replication and protein synthesis comparable to wild-type JEV, whereas propagation in Vero cells was impaired...
  32. ncbi The receptor-binding region of human apolipoprotein E has direct anti-infective activity
    Curtis B Dobson
    Faculty of Life Sciences, The University of Manchester, UK
    J Infect Dis 193:442-50. 2006
    ..The APOE genotype has a uniquely strong influence on the outcome of viral infection. The mechanism is unknown, although one possibility is direct inhibition of viral entry into cells...
  33. ncbi Cell culture (Vero) derived whole virus (H5N1) vaccine based on wild-type virus strain induces cross-protective immune responses
    Otfried Kistner
    Baxter AG, Biomedical Research Center, Uferstrasse 15, A 2304 Orth Donau, Austria
    Vaccine 25:6028-36. 2007
    ..These data indicate that cell culture-grown whole virus vaccines, based on the wild-type virus, allow the rapid high yield production of a candidate pandemic vaccine...
  34. ncbi An attenuated strain of enterovirus 71 belonging to genotype a showed a broad spectrum of antigenicity with attenuated neurovirulence in cynomolgus monkeys
    Minetaro Arita
    Department of Virology II, National Institute of Infectious Diseases, 4 7 1 Gakuen, Musashimurayama shi, Tokyo 208 0011, Japan
    J Virol 81:9386-95. 2007
    ..In the CNS, EV71(S1-3') was isolated only from the spinal cord. These results indicate that EV71(S1-3') acts as an effective antigen, although this attenuated strain was still neurotropic when inoculated via the intravenous route...
  35. ncbi Expression of Clostridium perfringens enterotoxin receptors claudin-3 and claudin-4 in prostate cancer epithelium
    H Long
    Departments of Medicine, Indiana University School of Medicine, Indianapolis, Indiana 46202, USA
    Cancer Res 61:7878-81. 2001
    ..The persistent high level of claudin-3 expression in prostate adenocarcinoma and functional cytotoxicity of CPE in metastatic androgen-independent prostate adenocarcinoma suggests a new potential therapeutic strategy for prostate cancer...
  36. ncbi A microcarrier cell culture process for propagating rabies virus in Vero cells grown in a stirred bioreactor under fully animal component free conditions
    Samia Rourou
    Viral Vaccines Research and Development Unit, Institut Pasteur, 13, Place Pasteur, BP 74, 1002 Tunis, Tunisia
    Vaccine 25:3879-89. 2007
    Rabies virus strain production in Vero cells grown on Cytodex 1 in a 2 L stirred tank bioreactor and in a medium free of components of human or animal origin (VP-SFM) is described...
  37. ncbi Characterization of homologous defective interfering RNA during persistent infection of Vero cells with Japanese encephalitis virus
    Sung Wook Yoon
    Department of Biology and Research Institute of Basic Sciences, Kyung Hee University, Seoul 130-701, Korea
    Mol Cells 21:112-20. 2006
    ..We identified a homolo-gous DI virus intimately associated with JEV persis-tence in Vero cells. The production of DI RNA during undiluted serial passages of JEV coincided with the appearance of cells ..
  38. ncbi Molecular and phenotypic analysis of a working seed lot of yellow fever virus 17DD vaccine strain produced from the secondary seed lot 102/84 with an additional passage in chicken embryos
    Renato S Marchevsky
    , , Avenida Brasil 4365, Rio de Janeiro, RJ 21040-900, Brazil
    Biologicals 34:191-7. 2006
    ..It is concluded that the additional chicken embryo passage did not lead to any genetic change and the new working seed virus retained its attenuation for monkeys comparable to the 102/84 reference virus...
  39. ncbi Herpes simplex virus gE/gI must accumulate in the trans-Golgi network at early times and then redistribute to cell junctions to promote cell-cell spread
    Aaron Farnsworth
    Department of Molecular Microbiology and Immunology, Oregon Health and Science University, 3181 SW Sam Jackson Park Rd, Portland, OR 97239, USA
    J Virol 80:3167-79. 2006
    ..Additionally, gE CT sequences between residues 495 and 519, which contain no obvious cell sorting motifs, are required to promote gE/gI traffic to cell junctions and cell-to-cell spread...
  40. ncbi Role of endosomal cathepsins in entry mediated by the Ebola virus glycoprotein
    Kathryn Schornberg
    Department of Microbiology, University of Virginia, 1300 Jefferson Park Ave, Charlottesville, Virginia 22908-0734, USA
    J Virol 80:4174-8. 2006
    ..These and other results support a model in which CatB and CatL prime GP by generating a 19-kDa intermediate that can be acted upon by an as yet unidentified endosomal/lysosomal enzyme to trigger fusion...
  41. ncbi Live attenuated influenza virus expressing human interleukin-2 reveals increased immunogenic potential in young and aged hosts
    Boris Ferko
    Institute of Applied Microbiology, University of Natural Resources and Applied Life Sciences, Muthgasse 18B, A-1190 Vienna, Austria
    J Virol 80:11621-7. 2006
    ..Our findings emphasize the potential of reverse genetics to improve the efficacy of live influenza vaccines, thus rendering them more suitable for high-risk age groups...
  42. ncbi Rapid detection of important human pathogenic Phleboviruses
    Manfred Weidmann
    Institute of Virology, University of Gottingen, Kreuzbergring 57, 37075 Gottingen, Germany
    J Clin Virol 41:138-42. 2008
    ..Rapid diagnostics are not available for several human pathogens in the genus Phlebovirus of the Bunyaviridae...
  43. ncbi Influenza B mutant viruses with truncated NS1 proteins grow efficiently in Vero cells and are immunogenic in mice
    Nina Wressnigg
    Avir Greenhills Biotechnology, Gersthoferstrasse 29 31, 1180 Vienna, Austria
    J Gen Virol 90:366-74. 2009
    ..Viable viruses containing the N-terminal 14, 38, 57 or 80 aa of the NS1 protein were rescued in Vero cells. The influenza B virus NS1-truncated mutants were impaired in their ability to counteract interferon (IFN) ..
  44. ncbi Quantitative proteomics using stable isotope labeling with amino acids in cell culture reveals changes in the cytoplasmic, nuclear, and nucleolar proteomes in Vero cells infected with the coronavirus infectious bronchitis virus
    Edward Emmott
    Institute of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds, Leeds, United Kingdom
    Mol Cell Proteomics 9:1920-36. 2010
    ..Here we detail a high throughput quantitative proteomics analysis of Vero cells infected with the coronavirus infectious bronchitis virus (IBV), a positive strand RNA virus that replicates in ..
  45. ncbi Assessment of immunogenic potential of Vero adapted formalin inactivated vaccine derived from novel ECSA genotype of Chikungunya virus
    Mugdha Tiwari
    Defence Research and Development Establishment, Gwalior, M P, India
    Vaccine 27:2513-22. 2009
    ....
  46. ncbi Anterograde spread of herpes simplex virus type 1 requires glycoprotein E and glycoprotein I but not Us9
    Helen M McGraw
    Division of Infectious Diseases, Department of Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 6073, USA
    J Virol 83:8315-26. 2009
    ..This finding contrasts with gE- and gI-deleted viruses, which displayed no anterograde spread in any animal model. Thus, gE and gI are essential for HSV-1 anterograde spread, while Us9 is dispensable...
  47. ncbi Incorporation of the green fluorescent protein into the herpes simplex virus type 1 capsid
    P Desai
    Virology Laboratories, Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Virol 72:7563-8. 1998
    ....
  48. ncbi During lytic infections, herpes simplex virus type 1 DNA is in complexes with the properties of unstable nucleosomes
    Jonathan J Lacasse
    Signal Transduction Research Group, Molecular Mechanisms of Growth Control Research Group, University of Alberta, 327C Heritage Medical Research Center, Edmonton, Alberta T6G 2S2, Canada
    J Virol 84:1920-33. 2010
    ..The HSV-1 DNAs in complexes fractionating as mono- to dinucleosomes were stabilized by cross-linking. Therefore, most HSV-1 DNA forms particularly unstable nucleosome-like complexes at 5 h of lytic infection...
  49. ncbi Modification and reorganization of the cytoprotective cellular chaperone Hsp27 during herpes simplex virus type 1 infection
    Shomita S Mathew
    Wadsworth Center, The David Axelrod Institute, New York State Department of Health, Albany, 12208, USA
    J Virol 83:9304-12. 2009
    ..Conversely, we find that phosphorylated forms of Hsp27 are localized exclusively in the cytoplasm. Last, in cells depleted of all forms of Hsp27, virus replication is significantly reduced...
  50. ncbi A novel function of heparan sulfate in the regulation of cell-cell fusion
    Christopher D O'Donnell
    Department of Ophthalmology and Visual Sciences and the Department of Microbiology and Immunology, College of Medicine, University of Illinois, Chicago, Illinois 60612, USA
    J Biol Chem 284:29654-65. 2009
    ..This could serve as a possible explanation as to why HS-deficient cells showed significantly enhanced cell-cell fusion and suggests that HS could regulate fusion via fine tuning of RhoA and Cdc42 activities...
  51. ncbi Characterization of mosquito-adapted West Nile virus
    Alexander T Ciota
    The Arbovirus Laboratories, Wadsworth Center, New York State Department of Health, 5668 State Farm Road, Slingerlands, NY 12159, USA
    J Gen Virol 89:1633-42. 2008
    ....
  52. ncbi A viral E3 ligase targets RNF8 and RNF168 to control histone ubiquitination and DNA damage responses
    Caroline E Lilley
    The Salk Institute for Biological Studies, La Jolla, CA 92037, USA
    EMBO J 29:943-55. 2010
    ..Our study raises the possibility that the ICP0-mediated control of histone ubiquitination may link DNA repair, relief of transcriptional repression, and activation of latent viral genomes...
  53. ncbi MDCK and Vero cells for influenza virus vaccine production: a one-to-one comparison up to lab-scale bioreactor cultivation
    Yvonne Genzel
    Max Planck Institute for Dynamics of Complex Technical Systems, Magdeburg, Germany
    Appl Microbiol Biotechnol 88:461-75. 2010
    Over the last decade, adherent MDCK (Madin Darby canine kidney) and Vero cells have attracted considerable attention for production of cell culture-derived influenza vaccines...
  54. ncbi Use of MDCK cells for production of live attenuated influenza vaccine
    Jonathan Liu
    MedImmune, LLC, 3055 Patrick Henry Drive, Santa Clara, CA 95054, United States
    Vaccine 27:6460-3. 2009
    ..Both Vero and MDCK cells could support a higher level of virus replication for certain strains, however, Vero cells only produced high titers when grown in the presence of serum...
  55. ncbi A SAP30 complex inhibits IFN-beta expression in Rift Valley fever virus infected cells
    Nicolas Le May
    Unite de Genetique Moleculaire des Bunyavirus, Institut Pasteur, Paris, France
    PLoS Pathog 4:e13. 2008
    ..We discuss here the strategy developed by the highly pathogenic RVFV to evade the host antiviral response, affecting nuclear organization and IFN-beta promoter chromatin structure...
  56. ncbi Preclinical evaluation of a replication-deficient intranasal DeltaNS1 H5N1 influenza vaccine
    Julia Romanova
    Avir Green Hills Biotechnology AG, Vienna, Austria
    PLoS ONE 4:e5984. 2009
    ..As a result, DeltaNS1 viruses elicit strong B- and T- cell mediated immune responses...
  57. ncbi Characterization of a Coxiella burnetii ftsZ mutant generated by Himar1 transposon mutagenesis
    Paul A Beare
    National Institute of Allergy and Infectious Diseases, Hamilton, Montana 59840, USA
    J Bacteriol 191:1369-81. 2009
    ..b>Vero cells were infected with electroporated C...
  58. ncbi The SARS-Coronavirus PLnc domain of nsp3 as a replication/transcription scaffolding protein
    Isabelle Imbert
    Centre National de la Recherche Scientifique and Universités d Aix Marseille I et II, UMR 6098, Architecture et Fonction des Macromolecules Biologiques, Ecole Supérieure d Ingénieurs de Luminy Case 925, Marseille Cedex 9, France
    Virus Res 133:136-48. 2008
    ..The interaction map thus provides a sum of the interactions that may occur at some point during coronavirus RNA synthesis and provides a framework for future research...
  59. ncbi The low lysine content of ricin A chain reduces the risk of proteolytic degradation after translocation from the endoplasmic reticulum to the cytosol
    Emma D Deeks
    Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, U.K
    Biochemistry 41:3405-13. 2002
    ..This effect could also be reversed by proteasomal inhibition. Our data support the hypothesis that the evolution of a low lysine content is a degradation-avoidance strategy for toxins that retrotranslocate from the ER...
  60. ncbi A role for the protease-sensitive loop region of Shiga-like toxin 1 in the retrotranslocation of its A1 domain from the endoplasmic reticulum lumen
    Paul LaPointe
    Department of Medical Biophysics, University of Toronto and the Ontario Cancer Institute, University Health Network, Canada
    J Biol Chem 280:23310-8. 2005
    ..in the context of the 251-residue long A(1) domain or affect the toxicity of the resulting Slt-I variants toward Vero cells in the context of the holotoxin...
  61. ncbi Export of a Toxoplasma gondii rhoptry neck protein complex at the host cell membrane to form the moving junction during invasion
    Sebastien Besteiro
    UMR 5235 CNRS, Université de Montpellier 2, Montpelier, France
    PLoS Pathog 5:e1000309. 2009
    ..Globally, these results point toward a model of MJ organization where the parasite would be secreting and inserting interacting components on either side of the MJ, both at the host and at its own plasma membranes...
  62. ncbi Complete genome analysis of 33 ecologically and biologically diverse Rift Valley fever virus strains reveals widespread virus movement and low genetic diversity due to recent common ancestry
    Brian H Bird
    Special Pathogens Branch, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road MS G 14, Atlanta, GA 30329, USA
    J Virol 81:2805-16. 2007
    ..In addition to insights into the evolution and ecology of RVF virus, these genomic data also provide a foundation for the design of molecular detection assays and prototype vaccines useful in combating this important disease...
  63. ncbi Herpes simplex virus-infected cell protein 0 blocks the silencing of viral DNA by dissociating histone deacetylases from the CoREST-REST complex
    Haidong Gu
    Marjorie B Kovler Viral Oncology Laboratories, University of Chicago, 910 East 58th Street, Chicago, IL 60637, USA
    Proc Natl Acad Sci U S A 104:17134-9. 2007
    ..1 pfu of virus per cell was 100-, 10-, and 10-fold higher, respectively, than those of R8501 mutant virus. In Vero cells, the yield of R8502 was identical with that of wild-type virus...
  64. ncbi Flow cytometry-based assay for titrating dengue virus
    C R Lambeth
    Department of Microbiology and Immunology, CB #7290, University of North Carolina, Chapel Hill, NC 27599, USA
    J Clin Microbiol 43:3267-72. 2005
    ..The novel FACS-based methods described here will facilitate laboratory studies of dengue...
  65. ncbi Structural basis of West Nile virus neutralization by a therapeutic antibody
    Grant E Nybakken
    Department of Pathology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Nature 437:764-9. 2005
    ..Collectively, our results suggest that a vaccine strategy targeting the dominant DIII epitope may elicit safe and effective immune responses against flaviviral diseases...
  66. ncbi Isolation of Nipah virus from Malaysian Island flying-foxes
    Kaw Bing Chua
    Department of Medical Microbiology, Faculty of Medicine, University of Malaya, 50603 Lumpur, Kuala, Malaysia
    Microbes Infect 4:145-51. 2002
    ..urine and one from a partially eaten fruit swab) that caused Nipah virus-like syncytial cytopathic effect in Vero cells and stained strongly with Nipah- and Hendra-specific antibodies were isolated...
  67. ncbi Protein kinase D-dependent trafficking of the large Herpes simplex virus type 1 capsids from the TGN to plasma membrane
    Gaudeline Rémillard-Labrosse
    Department of Pathology and Cell Biology, University of Montreal, Quebec, Canada
    Traffic 10:1074-83. 2009
    ..Lastly, given the apical release of HSV-1 in neurons, it opens up the possibility that this kinase might regulate some apical sorting...
  68. ncbi Mutations in the E9L polymerase gene of cidofovir-resistant vaccinia virus strain WR are associated with the drug resistance phenotype
    Richard S Kornbluth
    Department of Medicine, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0676, USA
    Antimicrob Agents Chemother 50:4038-43. 2006
    ..These findings suggest that drug therapy for poxviruses may be complicated by drug resistance but that treatment of the infection with currently known compounds is possible...
  69. ncbi Double-stranded RNA is produced by positive-strand RNA viruses and DNA viruses but not in detectable amounts by negative-strand RNA viruses
    Friedemann Weber
    Abteilung Virologie, Institut fur Medizinische Mikrobiologie und Hygiene, Universitat Freiburg, D 79008 Freiburg, Germany
    J Virol 80:5059-64. 2006
    ..Surprisingly, however, no dsRNA signals were detected for negative-strand RNA viruses. Thus, dsRNA is indeed a general feature of most virus groups, but negative-strand RNA viruses appear to be an exception to that rule...
  70. ncbi NSs protein of Rift Valley fever virus blocks interferon production by inhibiting host gene transcription
    Agnès Billecocq
    Unité de Génétique Moléculaire des Bunyaviridés, Institut Pasteur, Paris, France
    J Virol 78:9798-806. 2004
    ..The present results suggest that NSs, unlike other viral IFN antagonists, does not inhibit IFN-specific transcription factors but blocks IFN gene expression at a subsequent step...
  71. ncbi Chimeric alphavirus vaccine candidates for chikungunya
    Eryu Wang
    Center for Biodefense and Emerging Infectious Diseases, and Sealy Center for Vaccine Development, University of Texas Medical Branch, Galveston, TX 77555, United States
    Vaccine 26:5030-9. 2008
    ..Vaccinated mice were fully protected against disease and viremia after CHIKV challenge...
  72. ncbi Transcriptional coactivators are not required for herpes simplex virus type 1 immediate-early gene expression in vitro
    Sebla B Kutluay
    Graduate Program in Cell and Molecular Biology, Michigan State University, East Lansing, Michigan 48824, USA
    J Virol 83:3436-49. 2009
    ....
  73. ncbi Budding of Marburgvirus is associated with filopodia
    Larissa Kolesnikova
    Institute of Virology, Marburg Philipps University, Marburg, Germany
    Cell Microbiol 9:939-51. 2007
    ..As filopodia are in close contact with neighbouring cells, usurpation of these structures may facilitate spread of MARV to adjacent cells...
  74. ncbi Ebola virus-like particles protect from lethal Ebola virus infection
    Kelly L Warfield
    US Army Medical Research Institute of Infectious Diseases, Frederick, MD 21702, USA
    Proc Natl Acad Sci U S A 100:15889-94. 2003
    ..Together, our data suggest that eVLPs represent a promising vaccine candidate for protection against Ebola virus infections and a much needed tool to examine the genesis and nature of immune responses to Ebola virus...
  75. ncbi Multiple anti-interferon actions of the influenza A virus NS1 protein
    Georg Kochs
    Department of Virology, University of Freiburg, D 79008 Freiburg, Germany
    J Virol 81:7011-21. 2007
    ..Our results demonstrate the capacity of FLUAV NS1 to suppress the antiviral host defense at multiple levels and the existence of strain-specific differences that may modulate virus pathogenicity...
  76. ncbi Influenza virus evades innate and adaptive immunity via the NS1 protein
    Ana Fernandez-Sesma
    Department of Microbiology, Mount Sinai School of Medicine, One Gustave L. Levy Place, New York, NY 10029, USA
    J Virol 80:6295-304. 2006
    ..Our observations also support the potential use of NS1 mutant influenza viruses as live attenuated influenza virus vaccines...
  77. ncbi Conformational reorganization of the SARS coronavirus spike following receptor binding: implications for membrane fusion
    Daniel R Beniac
    Viral Diseases Division, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada
    PLoS ONE 2:e1082. 2007
    ..The SARS-CoV spike provides an ideal model system to study receptor binding and membrane fusion in the native state, employing cryo-electron microscopy and single-particle image analysis...
  78. ncbi Nectin-2-mediated entry of a syncytial strain of herpes simplex virus via pH-independent fusion with the plasma membrane of Chinese hamster ovary cells
    Mark G Delboy
    Department of Microbiology and Immunology, Medical College of Virginia at Virginia Commonwealth University, Richmond, Virginia, 23298 0678, USA
    Virol J 3:105. 2006
    ..The receptor requirements for HSV-induced FFWO are not known. We used the syncytial HSV-1 strain ANG path as a tool to evaluate the complex interplay between receptor usage, membrane fusion, and selection of entry pathway...
  79. ncbi Low pH-induced conformational change in herpes simplex virus glycoprotein B
    Stephen J Dollery
    1101 E Marshall St, Room 6 038, P O Box 980678, Richmond, VA 23298 0678
    J Virol 84:3759-66. 2010
    ..We propose that intracellular low pH induces alterations in gB conformation that, together with additional triggers such as receptor binding, are essential for virion-cell fusion during herpesviral entry by endocytosis...
  80. ncbi Overexpression of gK in gK-transformed cells collapses the Golgi apparatus into the endoplasmic reticulum inhibiting virion egress, glycoprotein transport, and virus-induced cell fusion
    Timothy P Foster
    Division of Biotechnology and Molecular Medicine, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803, USA
    Virology 317:237-52. 2003
    ..Therefore, virion entrapment within perinuclear spaces and inhibition of glycoprotein transport are due to gK-mediated collapse of the Golgi apparatus...
  81. ncbi Varicella-zoster virus ORF4 latency-associated protein is important for establishment of latency
    Jeffrey I Cohen
    Medical Virology Section, Laboratory of Clinical Infectious Diseases, National Institutes of Health, 10 Center Drive, Bethesda, MD 20892, USA
    J Virol 79:6969-75. 2005
    ..Thus, in addition to VZV ORF63, which was previously shown to be critical for efficient establishment of latency, ORF4 is also important for latent infection...
  82. ncbi Beta interferon and gamma interferon synergize to block viral DNA and virion synthesis in herpes simplex virus-infected cells
    Amy T Pierce
    Department of Microbiology and Immunology, Tulane University Medical School, New Orleans, LA 70112, USA
    J Gen Virol 86:2421-32. 2005
    ..To this end, multiple steps in HSV-1 infection were compared in populations of Vero cells infected with HSV-1 strain KOS (m.o.i. of 2...
  83. ncbi Characterization of the triplet repeats in the central domain of the gamma134.5 protein of herpes simplex virus 1
    Xianghong Jing
    Department of Microbiology and Immunology (M/C 790, College of Medicine, The University of Illinois at Chicago, 835 South Wolcott Avenue, Chicago, IL 60612, USA
    J Gen Virol 86:2411-9. 2005
    ..Accordingly, these mutant viruses are predominantly present within infected cells. These results indicate that deletions in the central domain of the gamma134.5 protein impair virus egress, but not virus response to interferon...
  84. ncbi Cinanserin is an inhibitor of the 3C-like proteinase of severe acute respiratory syndrome coronavirus and strongly reduces virus replication in vitro
    Lili Chen
    Drug Discovery and Design Center, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences
    J Virol 79:7095-103. 2005
    ..These findings demonstrate that the old drug cinanserin is an inhibitor of SARS-CoV replication, acting most likely via inhibition of the 3CL proteinase...
  85. ncbi State and role of SRC family kinases in replication of herpes simplex virus 1
    Yu Liang
    The Marjorie B Kovler Viral Oncology Laboratories, University of Chicago, 910 East 58th Street, Chicago IL 60637, USA
    J Virol 80:3349-59. 2006
    ..We conclude that HSV-1 proteins regulate the activities of Src family kinases to achieve optimal viral yields in the course of viral replication...
  86. ncbi Resveratrol suppresses nuclear factor-kappaB in herpes simplex virus infected cells
    Seth A Faith
    Department of Microbiology, Immunology and Biochemistry, Northeastern Ohio Universities College of Medicine, Rootstown, OH 44272, USA
    Antiviral Res 72:242-51. 2006
    ..determined that resveratrol, in a dose dependent and reversible manner, suppressed activation of NF-kappaB in Vero cells infected with HSV-1, HSV-2 and acyclovir resistant HSV-1...
  87. ncbi Synthesis of novel substituted 2-phenylpyrazolopyridines with potent activity against herpesviruses
    Kristjan S Gudmundsson
    Department of Medicinal Chemistry, GlaxoSmithKline Research and Development, Five Moore Drive, Research Triangle Park, NC 27709 3398, USA
    Bioorg Med Chem 13:5346-61. 2005
    ..Approaches to rapidly access a number of analogs with different 2-phenyl substitution patterns are outlined. Several of the compounds described have comparable activity to acyclovir against HSV-1 and HSV-2...
  88. ncbi The role of protein kinase activity expressed by the UL13 gene of herpes simplex virus 1: the activity is not essential for optimal expression of UL41 and ICP0
    Michiko Tanaka
    Department of Pathology, National Institute of Infectious Disease, Shinjuku ku, Tokyo 162 8640, Japan
    Virology 341:301-12. 2005
    ..These results indicate that the protein kinase activity of UL13 plays a role in viral replication in cell culture, but the activity is not essential for the optimal expression of UL41 and ICP0...
  89. ncbi Construction of recombinant herpes simplex virus type I expressing green fluorescent protein without loss of any viral genes
    Michiko Tanaka
    Department of Pathology, National Institute of Infectious Disease, 1-23-1 Toyama, Sinjyuku-ku, Tokyo 162-8640, Japan
    Microbes Infect 6:485-93. 2004
    ..ii) YK333 replicated as well as wild-type HSV-1 F strain in Vero cells. (iii) As one application of the recombinant YK333 for research on HSV-1, we developed a system to detect anti-..
  90. ncbi Antiherpetic mode of action of (22S,23S)-3beta-bromo-5alpha,22,23-trihydroxystigmastan-6-one in vitro
    Mónica B Wachsman
    Laboratorio de Virologia, Departamento de Quimica Biologica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellon 2, Piso 4, 1428 Buenos Aires, Argentina
    Int J Antimicrob Agents 23:524-6. 2004
    The replication of herpes simplex virus (HSV) type 1 in Vero cells is inhibited in the presence of (22S,23S)-3beta-bromo-5alpha,22,23-trihydroxystigmastan-6-one (6b), a synthetic brassinosteroid derivative...
  91. ncbi Suppression of herpes simplex virus 1 in MDBK cells via the interferon pathway
    Cristina Barreca
    Marie Curie Research Institute, Oxted, The Chart, Surrey RH8 0TL, United Kingdom
    J Virol 78:8641-53. 2004
    ..Compared to Vero cells, MDBK cells supported a normal productive infection at a high multiplicity with complete cell destruction...
  92. ncbi Herpes simplex virus type 1 ICP0 protein mediates activation of adeno-associated virus type 2 rep gene expression from a latent integrated form
    Marie-Claude Geoffroy
    INSERM U649, , , , 30 Bd Jean Monnet, 1, 44035 Nantes Cedex 01, France
    J Virol 78:10977-86. 2004
    ....
  93. ncbi Rapid determination of antiviral drug susceptibility of herpes simplex virus types 1 and 2 by real-time PCR
    Thuong Nguyen Thi
    Laboratoire de Virologie, , EA 2387, CERVI, , Paris 75013, France
    Antiviral Res 69:152-7. 2006
    ..The number of HSV DNA copies within Vero cells after 24 h infection was strongly correlated with the number of plaques obtained after 72 h infection...
  94. ncbi Anti-herpes virus activity of Dunbaria bella Prain
    Pannarat Akanitapichat
    Faculty of Pharmaceutical Sciences, Ubon Ratchathani, Ubon Ratchathani 34190, Thailand
    J Ethnopharmacol 105:64-8. 2006
    ..Furthermore, the antiviral activity was dependent on multiplicity of infection and the type of host cell. Preliminary chemical analysis showed that Fr.372 contained neither phenolic compounds nor alkaloids...
  95. ncbi Cell lines that support replication of a novel herpes simplex virus 1 UL31 deletion mutant can properly target UL34 protein to the nuclear rim in the absence of UL31
    Li Liang
    Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA
    Virology 329:68-76. 2004
    ..produced virus titers approximately 10- to 50-fold lower in rabbit skin cells, more than 2000-fold lower in Vero cells, and more than 1500-fold lower in CV1 cells, compared to a virus bearing a restored U(L)31 gene...
  96. ncbi Replication of herpes simplex virus 1 depends on the gamma 134.5 functions that facilitate virus response to interferon and egress in the different stages of productive infection
    Xianghong Jing
    Department of Microbiology and Immunology, College of Medicine, University of Illinois at Chicago, Chicago, IL 60612, USA
    J Virol 78:7653-66. 2004
    ..These results indicate that efficient viral replication depends on the gamma(1)34.5 functions required to prevent the PKR response and to facilitate virus egress in the different stages during virus infection...
  97. ncbi Herpes simplex virus type 1 gK is required for gB-mediated virus-induced cell fusion, while neither gB and gK nor gB and UL20p function redundantly in virion de-envelopment
    Jeffrey M Melancon
    Division of Biotechnology and Molecular Medicine, School of Veterinary Medicine, Louisiana State University, Baton Rouge, Louisiana 70803, USA
    J Virol 79:299-313. 2005
    ..Both viruses formed small nonsyncytial plaques on noncomplementing Vero cells and large syncytial plaques on gK-complementing cells, indicating that gK expression was necessary for gBsyn3- ..
  98. ncbi Contributions of the structural proteins of severe acute respiratory syndrome coronavirus to protective immunity
    Ursula J Buchholz
    Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892 8007, USA
    Proc Natl Acad Sci U S A 101:9804-9. 2004
    ....
  99. ncbi Anti-HSV activity of digitoxin and its possible mechanisms
    Chun Ting Su
    Department of Clinical Laboratory Sciences and Medical Biotechnology, National Taiwan University College of Medicine, Taipei, Taiwan
    Antiviral Res 79:62-70. 2008
    ..The observation that digitoxin can inhibit acyclovir-resistant viruses further implicates that digitoxin represents a novel drug class with distinct antiviral mechanisms from traditional drugs...
  100. ncbi The extracellular domain of herpes simplex virus gE is indispensable for efficient cell-to-cell spread: evidence for gE/gI receptors
    Katarina Polcicova
    L-220, Room 6366/BSc, Department of Molecular Microbiology and Immunology, Oregon Health and Science University, 3181 SW Sam Jackson Park Rd, Portland, OR 97239, USA
    J Virol 79:11990-2001. 2005
    ..This fits with similarities in structure and function of gE/gI and gD, which is a receptor binding protein...
  101. ncbi Antiviral mode of action of a synthetic brassinosteroid against Junin virus replication
    Viviana Castilla
    Laboratorio de Virologia, Departamento de Quimica Biologica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellon 2, Piso 4, 1428 Buenos Aires, Argentina
    Antiviral Res 68:88-95. 2005
    ..22S,23S)-3beta-bromo-5alpha,22,23-trihydroxystigmastan-6-one (6b) against Junin virus replication in Vero cells was investigated. Time-related experiments showed that 6b mainly affects an early event of virus growth cycle...

Research Grants69

  1. A SARS-CoV Spike Protein Vaccine
    B Sim; Fiscal Year: 2005
    ..Anti-sera will be assessed for antibodies to spike protein by ELISA and capacity to neutralize virus invasion of Vero cells in vitro...
  2. Developing a Paramyxovirus-based H5N1 Vaccine
    Biao He; Fiscal Year: 2010
    ..PIV5 can be produced in high titers (up to 8x108 plaque forming units (PFU)/ml) in many cells including Vero cells, which are WHO approved for vaccine production. PIV5 can infect human cell lines as well as primary human cells...
  3. Developing a Paramyxovirus-based H5N1 Vaccine
    Biao He; Fiscal Year: 2009
    ..PIV5 can be produced in high titers (up to 8x108 plaque forming units (PFU)/ml) in many cells including Vero cells, which are WHO approved for vaccine production. PIV5 can infect human cell lines as well as primary human cells...
  4. CHARACTERIZATION OF HCMV UL84
    Gregory Pari; Fiscal Year: 2009
    ..In Vero cells, IE2 apparently represses a high basal level of activation from this promoter...
  5. ACTIN BASED MOTILITY BY RICKETTSIA RICKETTSII
    ROBERT HEINZEN; Fiscal Year: 2002
    ..Transfected Vero cells producing specific domains of rOmpA or rOmpB, and infected with R...
  6. GLYCOLIPIDS AND HEMOLYTIC UREMIC SYNDROME
    David Haslam; Fiscal Year: 2002
    ..In preliminary studies VT-susceptible Vero cells were transfected with the cDNA encoding Forssman synthetase (FS)...
  7. STUDIES ON DIPHTHERIA TOXIN AND ITS RECEPTOR
    Leon Eidels; Fiscal Year: 1990
    ..fully and purify the specific diphtheria toxin-binding cell surface protein(s) from highly toxin-sensitive Vero cells. The diphtheria toxin-binding proteins are detected by i) prebinding toxin to extrinsically radiolabeled Vero ..
  8. CYTOMEGALOVIRUS DNA REPLICATION AND RECOMBINATION
    Edward Mocarski; Fiscal Year: 1993
    ..will be assayed by transfection of CMV-permissive human fibroblast and U373-MG cells as well as nonpermissive Vero cells and COS-7 cells, a cell line which will produce high levels of each of the products...
  9. Ab Alphavirus Replicon Vaccine against Cytomegalovirus
    Robert Esch; Fiscal Year: 2005
    ..Purified RNA transcripts produced from the replicon and helper plasmids will be electroporated into certified Vero cells using various amounts and electroporation parameters to identify the optimal conditions for transfection ..
  10. DIPHTHERIA TOXIN AND ITS RECEPTOR
    Leon Eidels; Fiscal Year: 1993
    ..to characterize fully the specific diphtheria toxin-binding cell surface protein(s) from highly toxin-sensitive Vero cells that function as the toxin receptor...
  11. Microcarrier culture system for human influenza vaccine
    WILLIAM HILLEGAS; Fiscal Year: 2004
    ..integrate critical cell culture and viral growth parameters for optimal production of human influenza virus in Vero cells grown in Hillex microcarrier-suspension culture. Specific Aim II...