vero cells

Summary

Summary: A CELL LINE derived from the kidney of the African green (vervet) monkey, (CERCOPITHECUS AETHIOPS) used primarily in virus replication studies and plaque assays.

Top Publications

  1. doi Identification and characterization of a cross-neutralization epitope of Enterovirus 71
    Chia Chyi Liu
    Vaccine R and D Center, National Health Research Institutes, Zhunan Town, Miaoli County 350, Taiwan
    Vaccine 29:4362-72. 2011
  2. ncbi Endosomal proteolysis of the Ebola virus glycoprotein is necessary for infection
    Kartik Chandran
    Department of Medicine, Brigham and Women s Hospital and Harvard Medical School, Boston, MA 02115, USA
    Science 308:1643-5. 2005
  3. pmc Purification and characterization of enterovirus 71 viral particles produced from vero cells grown in a serum-free microcarrier bioreactor system
    Chia Chyi Liu
    Vaccine Research and Development Center, National Health Research Institutes, Zhunan Town, Miaoli County, Taiwan
    PLoS ONE 6:e20005. 2011
  4. ncbi Herpes simplex virus-1 entry into cells mediated by a novel member of the TNF/NGF receptor family
    R I Montgomery
    Northwestern University Medical School, Department of Microbiology Immunology, Chicago, Illinois 60611, USA
    Cell 87:427-36. 1996
  5. pmc Role of endosomal cathepsins in entry mediated by the Ebola virus glycoprotein
    Kathryn Schornberg
    Department of Microbiology, University of Virginia, 1300 Jefferson Park Ave, Charlottesville, Virginia 22908 0734, USA
    J Virol 80:4174-8. 2006
  6. pmc Epidemiologic and virologic investigation of hand, foot, and mouth disease, southern Vietnam, 2005
    Phan Van Tu
    Pasteur Institute, Ho Chi Minh City, Vietnam
    Emerg Infect Dis 13:1733-41. 2007
  7. pmc Roles for endocytosis and low pH in herpes simplex virus entry into HeLa and Chinese hamster ovary cells
    Anthony V Nicola
    Medical Virology Section, Laboratory of Clinical Investigation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Virol 77:5324-32. 2003
  8. pmc Monoclonal antibodies that bind to domain III of dengue virus E glycoprotein are the most efficient blockers of virus adsorption to Vero cells
    W D Crill
    Arbovirus Disease Branch, Division of Vector Borne Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, U S Department of Health and Human Services, Fort Collins, Colorado 80522, USA
    J Virol 75:7769-73. 2001
  9. doi Selection and characterization of vaccine strain for Enterovirus 71 vaccine development
    Jui Yuan Chang
    Vaccine R and D Center, National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Zhunan Township, Miaoli County 350, Taiwan
    Vaccine 30:703-11. 2012
  10. pmc The endoplasmic reticulum provides the membrane platform for biogenesis of the flavivirus replication complex
    Leah K Gillespie
    Department of Microbiology, La Trobe University, Bundoora, Melbourne 3086, Australia
    J Virol 84:10438-47. 2010

Detail Information

Publications369 found, 100 shown here

  1. doi Identification and characterization of a cross-neutralization epitope of Enterovirus 71
    Chia Chyi Liu
    Vaccine R and D Center, National Health Research Institutes, Zhunan Town, Miaoli County 350, Taiwan
    Vaccine 29:4362-72. 2011
    ..Thus, VP2-28 could be used as the surrogate biomarker in the potency testing of candidate EV71 vaccines...
  2. ncbi Endosomal proteolysis of the Ebola virus glycoprotein is necessary for infection
    Kartik Chandran
    Department of Medicine, Brigham and Women s Hospital and Harvard Medical School, Boston, MA 02115, USA
    Science 308:1643-5. 2005
    ..CatB and CatB/CatL inhibitors diminish the multiplication of infectious EboV-Zaire in cultured cells and may merit investigation as anti-EboV drugs...
  3. pmc Purification and characterization of enterovirus 71 viral particles produced from vero cells grown in a serum-free microcarrier bioreactor system
    Chia Chyi Liu
    Vaccine Research and Development Center, National Health Research Institutes, Zhunan Town, Miaoli County, Taiwan
    PLoS ONE 6:e20005. 2011
    ..Enterovirus 71 (EV71) infections manifest most commonly as a childhood exanthema known as hand-foot-and-mouth disease (HFMD) and can cause neurological disease during acute infection...
  4. ncbi Herpes simplex virus-1 entry into cells mediated by a novel member of the TNF/NGF receptor family
    R I Montgomery
    Northwestern University Medical School, Department of Microbiology Immunology, Chicago, Illinois 60611, USA
    Cell 87:427-36. 1996
    ..The contribution of HVEM to HSV entry into human cells was demonstrable in activated T cells. HVEM, the first identified mediator of HSV entry, is a new member of the TNF/NGF receptor family...
  5. pmc Role of endosomal cathepsins in entry mediated by the Ebola virus glycoprotein
    Kathryn Schornberg
    Department of Microbiology, University of Virginia, 1300 Jefferson Park Ave, Charlottesville, Virginia 22908 0734, USA
    J Virol 80:4174-8. 2006
    ..These and other results support a model in which CatB and CatL prime GP by generating a 19-kDa intermediate that can be acted upon by an as yet unidentified endosomal/lysosomal enzyme to trigger fusion...
  6. pmc Epidemiologic and virologic investigation of hand, foot, and mouth disease, southern Vietnam, 2005
    Phan Van Tu
    Pasteur Institute, Ho Chi Minh City, Vietnam
    Emerg Infect Dis 13:1733-41. 2007
    ..In the second half of the year, viruses belonging to subgenogroup C5 predominated during a period of higher HEV71 activity...
  7. pmc Roles for endocytosis and low pH in herpes simplex virus entry into HeLa and Chinese hamster ovary cells
    Anthony V Nicola
    Medical Virology Section, Laboratory of Clinical Investigation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Virol 77:5324-32. 2003
    Herpes simplex virus (HSV) infection of many cultured cells, e.g., Vero cells, can be initiated by receptor binding and pH-neutral fusion with the cell surface...
  8. pmc Monoclonal antibodies that bind to domain III of dengue virus E glycoprotein are the most efficient blockers of virus adsorption to Vero cells
    W D Crill
    Arbovirus Disease Branch, Division of Vector Borne Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, U S Department of Health and Human Services, Fort Collins, Colorado 80522, USA
    J Virol 75:7769-73. 2001
    ..set of anti-dengue (DEN) virus E-glycoprotein-specific monoclonal antibodies (MAbs) to block virus adsorption to Vero cells. In contrast to previous studies, the binding sites of these MAbs were localized to one of three structural ..
  9. doi Selection and characterization of vaccine strain for Enterovirus 71 vaccine development
    Jui Yuan Chang
    Vaccine R and D Center, National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Zhunan Township, Miaoli County 350, Taiwan
    Vaccine 30:703-11. 2012
    ..Therefore, these results provide valuable information for cell-based EV71 vaccine development...
  10. pmc The endoplasmic reticulum provides the membrane platform for biogenesis of the flavivirus replication complex
    Leah K Gillespie
    Department of Microbiology, La Trobe University, Bundoora, Melbourne 3086, Australia
    J Virol 84:10438-47. 2010
    ..In addition, we provide data that indicate that a majority of the viral RNA species housed within the RC is in a double-stranded RNA (dsRNA) form...
  11. pmc Inhibition of interferon signaling by dengue virus
    Jorge L Munoz-Jordan
    Department of Microbiology, Mount Sinai School of Medicine, One Gustave Levy Place, New York, NY 10029
    Proc Natl Acad Sci U S A 100:14333-8. 2003
    ..This protein, encoded by a positive strand RNA virus, is implicated as an IFN-signaling inhibitor...
  12. ncbi Image-guided radiovirotherapy for multiple myeloma using a recombinant measles virus expressing the thyroidal sodium iodide symporter
    David Dingli
    Molecular Medicine Program, Mayo Clinic and Foundation, Rochester, MN 55905, USA
    Blood 103:1641-6. 2004
    ..Testing in other radiosensitive cancers is warranted...
  13. pmc Small rho GTPases and cholesterol biosynthetic pathway intermediates in African swine fever virus infection
    Jose I Quetglas
    Departamento de Biotecnologia Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria, INIA, Madrid, Spain
    J Virol 86:1758-67. 2012
    ....
  14. pmc A monoclonal immunoglobulin G antibody directed against an immunodominant linear epitope on the ricin A chain confers systemic and mucosal immunity to ricin
    Lori M Neal
    Division of Infectious Disease, Wadsworth Center, New York State Department of Health, Albany, New York 12208, USA
    Infect Immun 78:552-61. 2010
    ..The potential of GD12 to serve as a therapeutic following ricin challenge was not explored in this study...
  15. pmc Endoproteolytic processing of the lymphocytic choriomeningitis virus glycoprotein by the subtilase SKI-1/S1P
    Winfried R Beyer
    Heinrich Pette Institut fur experimentelle Virologie und Immunologie an der Universitat Hamburg, D 20251 Hamburg, Germany
    J Virol 77:2866-72. 2003
    ..In agreement with the identified consensus motif, we show that the cellular subtilase SKI-1/S1P cleaves LCMV GP...
  16. pmc Ebolavirus is internalized into host cells via macropinocytosis in a viral glycoprotein-dependent manner
    Asuka Nanbo
    Influenza Research Institute, Department of Pathological Sciences, School of Veterinary Medicine, University of Wisconsin Madison, Madison, Wisconsin, United States of America
    PLoS Pathog 6:e1001121. 2010
    ..These findings provide new insights into the lifecycle of Ebolavirus and may aid in the development of therapeutics for Ebolavirus infection...
  17. pmc Dengue virus ensures its fusion in late endosomes using compartment-specific lipids
    Elena Zaitseva
    Section on Membrane Biology, Laboratory of Cellular and Molecular Biophysics, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, United States of America
    PLoS Pathog 6:e1001131. 2010
    ....
  18. ncbi Conserved receptor-binding domains of Lake Victoria marburgvirus and Zaire ebolavirus bind a common receptor
    Jens H Kuhn
    Department of Microbiology and Molecular Genetics, Harvard Medical School, New England Primate Research Center, Southborough, Massachusetts 01772, USA
    J Biol Chem 281:15951-8. 2006
    ..These studies identify the receptor-binding domains of both viruses, indicate that these viruses utilize a common receptor, and suggest that a single small molecule or vaccine can be developed to inhibit infection of all filoviruses...
  19. pmc Cellular entry of ebola virus involves uptake by a macropinocytosis-like mechanism and subsequent trafficking through early and late endosomes
    Mohammad F Saeed
    Department of Microbiology and Immunology, The University of Texas Medical Branch, Galveston, Texas, United States of America
    PLoS Pathog 6:e1001110. 2010
    ..similar virus-like particles in specific infection and entry assays to demonstrate that in HEK293T and Vero cells internalization of ZEBOV is independent of clathrin, caveolae, and dynamin...
  20. ncbi Quantitation of flaviviruses by fluorescent focus assay
    Anne F Payne
    The Arbovirus Laboratories, Wadsworth Center, New York State Department of Health, 5668 State Farm Rd, Slingerlands, NY 12159, USA
    J Virol Methods 134:183-9. 2006
    ..Louis encephalitis virus (SLEV), and Dengue virus (DENV) types 1-4. Vero cells were plated in 8-well chamber slides, and infected with 10-fold serial dilutions of virus...
  21. ncbi The antiviral activity of sulfated polysaccharides against dengue virus is dependent on virus serotype and host cell
    L B Talarico
    Laboratorio de Virologia, Departamento de Quimica Biologica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
    Antiviral Res 66:103-10. 2005
    ..Both seaweed derivatives were selective inhibitors of DENV-2 multiplication in Vero cells with inhibitory concentration 50% (IC50) values around 1 microg/ml and selectivity indices > 1000...
  22. ncbi High immunogenic enterovirus 71 strain and its production using serum-free microcarrier Vero cell culture
    Chia Chyi Liu
    Institute of Biotechnology, Department of Life Science, National Tsing Hua University, Hsinchu, Taiwan
    Vaccine 25:19-24. 2007
    ..Therefore, serum-free microcarrier culture is a valuable process for developing inactivated EV71 vaccines...
  23. ncbi Use of the red fluorescent protein as a marker of Kaposi's sarcoma-associated herpesvirus lytic gene expression
    Jeffrey Vieira
    Department of Laboratory Medicine, University of Washington, Seattle, WA 98109 8070, USA
    Virology 325:225-40. 2004
    ..rKSHV.219 from JSC-1 cells was used to infect Vero cells for purification of the recombinant virus. Vero cells were also used for the production of rKSHV...
  24. pmc Double-stranded RNA is produced by positive-strand RNA viruses and DNA viruses but not in detectable amounts by negative-strand RNA viruses
    Friedemann Weber
    Abteilung Virologie, Institut fur Medizinische Mikrobiologie und Hygiene, Universitat Freiburg, D 79008 Freiburg, Germany
    J Virol 80:5059-64. 2006
    ..Surprisingly, however, no dsRNA signals were detected for negative-strand RNA viruses. Thus, dsRNA is indeed a general feature of most virus groups, but negative-strand RNA viruses appear to be an exception to that rule...
  25. pmc Analysis of Rab GTPase-activating proteins indicates that Rab1a/b and Rab43 are important for herpes simplex virus 1 secondary envelopment
    Helen L Zenner
    Division of Virology, Department of Pathology, University of Cambridge, Cambridge CB2 1QP, UK
    J Virol 85:8012-21. 2011
    ....
  26. pmc The Ebola virus ribonucleoprotein complex: a novel VP30-L interaction identified
    A Groseth
    National Laboratory for Zoonotic Diseases and Special Pathogens, National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg R3E 3R2, Canada
    Virus Res 140:8-14. 2009
    ..The identification of these interactions allows a preliminary model of the EBOV RNP complex structure to be proposed, and may provide insight into filovirus transcriptional regulation...
  27. pmc NS1 protein of influenza A virus down-regulates apoptosis
    O P Zhirnov
    D I Ivanovsky Institute of Virology, Moscow 123098, Russia
    J Virol 76:1617-25. 2002
    ..As a consequence, delNS1 virus was also more lethal for chicken embryos than WT virus. In IFN-deficient Vero cells, however, apoptosis was delayed and developed with similar intensity after infection with both viruses...
  28. pmc Dengue virus type 1 nonstructural glycoprotein NS1 is secreted from mammalian cells as a soluble hexamer in a glycosylation-dependent fashion
    M Flamand
    Unité des Arbovirus et Virus des Fièvres Hémorragiques, Institut Pasteur, 75724 Paris Cedex 15, 91198 Gif sur Yvette Cedex, France
    J Virol 73:6104-10. 1999
    ..These results, together with observations related to the absence of secretion of NS1 from Den-infected insect cells, suggest that maturation and secretion of hexameric NS1 depend on the glycosylation status of the host cell...
  29. pmc Human macrophage C-type lectin specific for galactose and N-acetylgalactosamine promotes filovirus entry
    Ayato Takada
    Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Tokyo 108 8639, Japan
    J Virol 78:2943-7. 2004
    ..We propose that filoviruses use different C-type lectins to gain cellular entry, depending on the cell type, and promote efficient viral replication...
  30. ncbi High CD46 receptor density determines preferential killing of tumor cells by oncolytic measles virus
    Bambi D Anderson
    Molecular Medicine Program, Mayo Clinic Cancer Center, Mayo Clinic College of Medicine, 200 First Street SW, Rochester, MN 55905, USA
    Cancer Res 64:4919-26. 2004
    ..Discrimination between high and low CD46 receptor density provides a compelling basis for the oncolytic specificity of MV-Edm and establishes MV-Edm as a promising CD46-targeted cancer therapeutic agent...
  31. pmc Incorporation of the green fluorescent protein into the herpes simplex virus type 1 capsid
    P Desai
    Virology Laboratories, Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Virol 72:7563-8. 1998
    ....
  32. pmc Characterization of Ebola virus entry by using pseudotyped viruses: identification of receptor-deficient cell lines
    R J Wool-Lewis
    Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia 19104 6076, USA
    J Virol 72:3155-60. 1998
    ....
  33. pmc The Lassa virus glycoprotein precursor GP-C is proteolytically processed by subtilase SKI-1/S1P
    O Lenz
    Institute of Virology, Philipps University, Robert Koch Strasse 17, 35037 Marburg, Germany
    Proc Natl Acad Sci U S A 98:12701-5. 2001
    ..To our knowledge, there have been no previous reports of this type of viral glycoprotein processing, one that may be an interesting target for antiviral therapy...
  34. ncbi Contribution of the respiratory syncytial virus G glycoprotein and its secreted and membrane-bound forms to virus replication in vitro and in vivo
    M N Teng
    Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, 7 Center Dr. MSC 0720, Bethesda, Maryland 20892-0720, USA
    Virology 289:283-96. 2001
    ..In Vero cells, the DeltaG virus formed plaques and grew as efficiently as wild-type rRSV and mG...
  35. pmc Human membrane cofactor protein (CD46) acts as a cellular receptor for measles virus
    D Naniche
    CNRS ENS Lyon UMR 49, France
    J Virol 67:6025-32. 1993
    ..These results show that the human CD46 molecule serves as an MV receptor allowing virus-cell binding, fusion, and viral replication and open new perspectives in the study of MV pathogenesis...
  36. pmc Cellular and viral requirements for rapid endocytic entry of herpes simplex virus
    Anthony V Nicola
    Medical Virology Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892 1888, USA
    J Virol 78:7508-17. 2004
    ..Thus, similar to entry via direct penetration at the cell surface, HSV entry into cells by wortmannin-sensitive endocytosis is efficient, involves rapid cellular uptake of viral particles, and requires gB, gD, and gH-gL...
  37. pmc A SAP30 complex inhibits IFN-beta expression in Rift Valley fever virus infected cells
    Nicolas Le May
    Unite de Genetique Moleculaire des Bunyavirus, Institut Pasteur, Paris, France
    PLoS Pathog 4:e13. 2008
    ..We discuss here the strategy developed by the highly pathogenic RVFV to evade the host antiviral response, affecting nuclear organization and IFN-beta promoter chromatin structure...
  38. pmc Influenza virus evades innate and adaptive immunity via the NS1 protein
    Ana Fernandez-Sesma
    Department of Microbiology, Mount Sinai School of Medicine, One Gustave L Levy Place, New York, NY 10029, USA
    J Virol 80:6295-304. 2006
    ..Our observations also support the potential use of NS1 mutant influenza viruses as live attenuated influenza virus vaccines...
  39. pmc Blocking of interferon-induced Jak-Stat signaling by Japanese encephalitis virus NS5 through a protein tyrosine phosphatase-mediated mechanism
    Ren Jye Lin
    Institute of Biomedical Sciences, Academia Sinica, Nankang, Taipei 11529, Taiwan, Republic of China
    J Virol 80:5908-18. 2006
    ..We suggest that JEV NS5 is an IFN antagonist and that it may play a role in blocking IFN-stimulated Jak-Stat signaling via activation of PTPs during JEV infection...
  40. pmc Assembly and maturation of the flavivirus Kunjin virus appear to occur in the rough endoplasmic reticulum and along the secretory pathway, respectively
    J M MacKenzie
    Clinical Medical Virology Centre, University of Queensland, St Lucia, Queensland 4072, Australia
    J Virol 75:10787-99. 2001
    ..The results suggest that the assembly of virions occurs within the lumen of the RER and that subsequent maturation occurs via the secretory pathway...
  41. pmc A forward genetic strategy reveals destabilizing mutations in the Ebolavirus glycoprotein that alter its protease dependence during cell entry
    Anthony C Wong
    Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    J Virol 84:163-75. 2010
    ..Altogether, our findings suggest that CatB cleavage is required to facilitate the triggering of viral membrane fusion by destabilizing the prefusion conformation of EBOV GP...
  42. ncbi Analysis of filovirus entry into vero e6 cells, using inhibitors of endocytosis, endosomal acidification, structural integrity, and cathepsin (B and L) activity
    Anthony Sanchez
    Special Pathogens Branch, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
    J Infect Dis 196:S251-8. 2007
    ..These observations obtained using wild-type viruses confirm the results of studies utilizing recombinant systems and offer additional insights into filovirus entry...
  43. pmc Proteolysis of the Ebola virus glycoproteins enhances virus binding and infectivity
    Rachel L Kaletsky
    Department of Microbiology, University of Pennsylvania School of Medicine, 225 Johnson Pavilion, 3610 Hamilton Walk, Philadelphia, PA 19104 6076, USA
    J Virol 81:13378-84. 2007
    ..The influence of CatL in Ebola virus GP receptor binding should be useful in future studies characterizing the mechanism of Ebola virus entry...
  44. ncbi Dual therapy of ovarian cancer using measles viruses expressing carcinoembryonic antigen and sodium iodide symporter
    Kosei Hasegawa
    Molecular Medicine Program, Mayo Clinic College of Medicine, Rochester, Minnesota 55905, USA
    Clin Cancer Res 12:1868-75. 2006
    ..Because of the unfavorable replication kinetics of measles viruses expressing both CEA and NIS, we explored the feasibility of combining MV-CEA with MV-NIS for comprehensive virotherapy monitoring in ovarian cancer...
  45. pmc Dynamin- and clathrin-dependent endocytosis in African swine fever virus entry
    Bruno Hernaez
    Departamento de Biotecnologia Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria, INIA, Autovia A6 km 7, 28040 Madrid, Spain
    J Virol 84:2100-9. 2010
    ..These results suggest a dynamin-dependent and clathrin-mediated endocytic pathway of ASFV entry for the cell types and viral strains analyzed...
  46. pmc Vesicular stomatitis virus-based Ebola vaccines with improved cross-protective efficacy
    Andrea Marzi
    Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institute of Health, Hamilton, Montana 59840, USA
    J Infect Dis 204:S1066-74. 2011
    ..Our data demonstrate that cross-protection between the EBOV species can be achieved, although EBOV-GP alone cannot induce the required immune response...
  47. pmc Reverse genetics with a full-length infectious cDNA of severe acute respiratory syndrome coronavirus
    Boyd Yount
    Department of Epidemiology and Microbiology, University of North Carolina, Chapel Hill, NC 27599 7435, USA
    Proc Natl Acad Sci U S A 100:12995-3000. 2003
    ....
  48. pmc Heterocellular induction of interferon by negative-sense RNA viruses
    S Chen
    School of Biology, Centre for Biomolecular Sciences, BMS Building, North Haugh, University of St Andrews, St Andrews, Fife, KY16 9ST, UK
    Virology 407:247-55. 2010
    ..We show that only a limited number of infected cells are responsible for the production of IFN, and that this heterocellular production is a feature of the infecting virus as opposed to an intrinsic property of the cells...
  49. doi Generation of murine monoclonal antibodies which cross-neutralize human enterovirus genogroup B isolates
    Hsuen Wen Chang
    Vaccine Research and Development Center, National Health Research Institutes, Zhunan Township, Miaoli County 350, Taiwan
    J Virol Methods 173:189-95. 2011
    A live enterovirus 71 (EV71) isolate designated, EV71/E59, with genotype B4 produced in Vero cells and purified over a sucrose gradient was used as the immunogen to generate EV71-specific murine monoclonal antibodies...
  50. doi Efficacy, safety, and immunogenicity of a Vero-cell-culture-derived trivalent influenza vaccine: a multicentre, double-blind, randomised, placebo-controlled trial
    P Noel Barrett
    Global Research and Development, Baxter BioScience, Biomedical Research Centre, Orth Donau, Austria
    Lancet 377:751-9. 2011
    ..We investigated the safety, immunogenicity, and protective efficacy of a Vero-cell-culture-derived influenza vaccine, and assessed the correlation between vaccine efficacy and haemagglutination inhibition antibody titre...
  51. doi Antiviral activity of quercetin 7-rhamnoside against porcine epidemic diarrhea virus
    Hwa Jung Choi
    Natural Medicines Research Centre, Korea Research Institute of Bioscience and Biotechnology, Taejon 305 333, Republic of Korea
    Antiviral Res 81:77-81. 2009
    ....
  52. ncbi Cholesterol manipulation by West Nile virus perturbs the cellular immune response
    Jason M Mackenzie
    School of Molecular and Microbial Sciences, University of Queensland, Brisbane, Queensland 4072, Australia
    Cell Host Microbe 2:229-39. 2007
    ....
  53. pmc A ferret model of canine distemper virus virulence and immunosuppression
    Veronika von Messling
    Molecular Medicine Program, Mayo Clinic, Rochester, Minnesota 55905, USA
    J Virol 77:12579-91. 2003
    ..Thus, virulence-inducing mutations have accumulated throughout the genome...
  54. pmc Processing of the Ebola virus glycoprotein by the proprotein convertase furin
    V E Volchkov
    Institut fur Virologie, Philipps Universitat Marburg, 35011 Marburg, Germany
    Proc Natl Acad Sci U S A 95:5762-7. 1998
    ..As a result of these observations, it should now be considered that proteolytic processing of GP may be an important determinant for the pathogenicity of Ebola virus...
  55. pmc Ebola virus VP30-mediated transcription is regulated by RNA secondary structure formation
    Michael Weik
    Institut für Virologie der Philipps Universität Marburg, 35037 Marburg, Germany
    J Virol 76:8532-9. 2002
    ..Our results suggest that VP30 regulates a very early step of EBOV transcription, most likely by inhibiting pausing of the transcription complex at the RNA structure of the first transcription start site...
  56. pmc Caspase 3 activation is essential for efficient influenza virus propagation
    Walter J Wurzer
    Institute of Molecular Medicine IMM, Heinrich Heine Universitat, Universitatsstrasse 1, D 40225 Dusseldorf, Germany
    EMBO J 22:2717-28. 2003
    ..Our findings indicate that caspase 3 activation during the onset of apoptosis is a crucial event for efficient influenza virus propagation...
  57. pmc West Nile virus inhibits the signal transduction pathway of alpha interferon
    Ju Tao Guo
    Institute for Cancer Research, Fox Chase Cancer Center, 333 Cottman Ave, Philadelphia, PA 19111, USA
    J Virol 79:1343-50. 2005
    ..Moreover, we demonstrated that the viral nonstructural proteins are responsible for this effect. Thus, our results provided an explanation for the observed resistance of WNV to IFN-alpha in cells of vertebrate origin...
  58. ncbi Structural basis of West Nile virus neutralization by a therapeutic antibody
    Grant E Nybakken
    Department of Pathology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Nature 437:764-9. 2005
    ..Collectively, our results suggest that a vaccine strategy targeting the dominant DIII epitope may elicit safe and effective immune responses against flaviviral diseases...
  59. pmc The NS5 protein of the virulent West Nile virus NY99 strain is a potent antagonist of type I interferon-mediated JAK-STAT signaling
    Maudry Laurent-Rolle
    Laboratory of Virology, Rocky Mountain Laboratories, 903 S Fourth St, Hamilton, MT 59840, USA
    J Virol 84:3503-15. 2010
    ..Thus, a naturally occurring mutation is associated with the function of NS5 in IFN antagonism and may influence virulence of WNV field isolates...
  60. ncbi A mouse model for evaluation of prophylaxis and therapy of Ebola hemorrhagic fever
    M Bray
    Division of Virology, US Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland 21702 5011, USA
    J Infect Dis 178:651-61. 1998
    ..Mice injected peripherally with mouse-adapted or intraperitoneally with non-adapted EBO-Z virus resisted subsequent challenge with mouse-adapted virus...
  61. ncbi Isolation of Nipah virus from Malaysian Island flying-foxes
    Kaw Bing Chua
    Department of Medical Microbiology, Faculty of Medicine, University of Malaya, 50603 Lumpur, Kuala, Malaysia
    Microbes Infect 4:145-51. 2002
    ..urine and one from a partially eaten fruit swab) that caused Nipah virus-like syncytial cytopathic effect in Vero cells and stained strongly with Nipah- and Hendra-specific antibodies were isolated...
  62. ncbi Optimization of microcarrier cell culture process for the inactivated enterovirus type 71 vaccine development
    Suh Chin Wu
    Department of Life Science, Institute of Biotechnology, National Tsing Hua University, Hsinchu, Taiwan
    Vaccine 22:3858-64. 2004
    ..In this paper, Vero cells were found to produce higher titers of EV71 than did MRC-5 and WI-38 cells...
  63. pmc Phase I trial of intraperitoneal administration of an oncolytic measles virus strain engineered to express carcinoembryonic antigen for recurrent ovarian cancer
    Evanthia Galanis
    Division of Medical Oncology, Department of Molecular Medicine, Mayo Clinic, Gonda 10 141, 200 First Street Southwest, Rochester, MN 55905, USA
    Cancer Res 70:875-82. 2010
    ..Our findings indicate that i.p. administration of MV-CEA is well tolerated and results in dose-dependent biological activity in a cohort of heavily pretreated recurrent ovarian cancer patients...
  64. ncbi GP mRNA of Ebola virus is edited by the Ebola virus polymerase and by T7 and vaccinia virus polymerases
    V E Volchkov
    State Research Centre of Virology and Biotechnology Vector, Institute of Molecular Biology, Koltsovo, Novosibirsk Region, Russia
    Virology 214:421-30. 1995
    ..Our data indicate that the editing site in the Ebola virus glycoprotein gene is recognized not only by Ebola virus polymerase but also by DNA-dependent RNA polymerases of different origin...
  65. pmc An attenuated strain of enterovirus 71 belonging to genotype a showed a broad spectrum of antigenicity with attenuated neurovirulence in cynomolgus monkeys
    Minetaro Arita
    Department of Virology II, National Institute of Infectious Diseases, 4 7 1 Gakuen, Musashimurayama shi, Tokyo 208 0011, Japan
    J Virol 81:9386-95. 2007
    ..In the CNS, EV71(S1-3') was isolated only from the spinal cord. These results indicate that EV71(S1-3') acts as an effective antigen, although this attenuated strain was still neurotropic when inoculated via the intravenous route...
  66. ncbi SLAM (CDw150) is a cellular receptor for measles virus
    H Tatsuo
    Department of Virology, Faculty of Medicine, Kyushu University, Fukuoka, Japan
    Nature 406:893-7. 2000
    ..The identification of SLAM as a receptor for measles virus opens the way to a better understanding of the pathogenesis of measles virus infection, especially the immunosuppression induced by measles virus...
  67. pmc A double-inactivated severe acute respiratory syndrome coronavirus vaccine provides incomplete protection in mice and induces increased eosinophilic proinflammatory pulmonary response upon challenge
    Meagan Bolles
    Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA
    J Virol 85:12201-15. 2011
    ....
  68. doi Regulation of the p38 mitogen-activated protein kinase and dual-specificity phosphatase 1 feedback loop modulates the induction of interleukin 6 and 8 in cells infected with coronavirus infectious bronchitis virus
    Ying Liao
    School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, Singapore
    Virology 420:106-16. 2011
    ....
  69. pmc Microtubule-mediated transport of incoming herpes simplex virus 1 capsids to the nucleus
    B Sodeik
    Yale University School of Medicine, Department of Cell Biology, New Haven, Connecticut 06520 8002, USA
    J Cell Biol 136:1007-21. 1997
    ..presence and absence of microtubule depolymerizing agents, it was shown that the cytosolic capsid transport in Vero cells was mediated by microtubules...
  70. pmc Single HA2 mutation increases the infectivity and immunogenicity of a live attenuated H5N1 intranasal influenza vaccine candidate lacking NS1
    Brigitte M Krenn
    Avir Green Hills Biotechnology AG, Vienna, Austria
    PLoS ONE 6:e18577. 2011
    ..2). We hypothesized that the sensitivity of H5 HA to the acidic environment might be the reason for the low infectivity and immunogenicity of intranasal H5N1 vaccines for mammals...
  71. pmc A specific interaction of small molecule entry inhibitors with the envelope glycoprotein complex of the Junín hemorrhagic fever arenavirus
    Celestine J Thomas
    Center for Biomolecular Structure and Dynamics, University of Montana, Missoula, Montana 59812, USA
    J Biol Chem 286:6192-200. 2011
    ..This work expands our mechanistic understanding of arenavirus entry and provides a foundation to guide the development of small molecule compounds for the treatment of arenavirus hemorrhagic fevers...
  72. pmc The cellular RNA helicase DDX1 interacts with coronavirus nonstructural protein 14 and enhances viral replication
    Linghui Xu
    Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore
    J Virol 84:8571-83. 2010
    ..This study reveals that DDX1 contributes to efficient coronavirus replication in cell culture...
  73. pmc Multiple anti-interferon actions of the influenza A virus NS1 protein
    Georg Kochs
    Department of Virology, University of Freiburg, D 79008 Freiburg, Germany
    J Virol 81:7011-21. 2007
    ..Our results demonstrate the capacity of FLUAV NS1 to suppress the antiviral host defense at multiple levels and the existence of strain-specific differences that may modulate virus pathogenicity...
  74. pmc Cellular entry of hantaviruses which cause hemorrhagic fever with renal syndrome is mediated by beta3 integrins
    I N Gavrilovskaya
    Department of Medicine, State University of New York at Stony Brook, Stony Brook, New York 11794, USA
    J Virol 73:3951-9. 1999
    ..Since beta3 integrins regulate vascular permeability and platelet function, these findings also correlate beta3 integrin usage with common elements of hantavirus pathogenesis...
  75. pmc Inhibition of retinoic acid-inducible gene I-mediated induction of beta interferon by the NS1 protein of influenza A virus
    Masaki Mibayashi
    Department of Microbiology, Mount Sinai School of Medicine, 1 Gustave L Levy Place, New York, NY 10029, USA
    J Virol 81:514-24. 2007
    ..Our results indicate that, in addition to sequestering dsRNA, the NS1 of influenza A virus binds to RIG-I and inhibits downstream activation of IRF-3, preventing the transcriptional induction of IFN-beta...
  76. doi Flavone enhances dengue virus type-2 (NGC strain) infectivity and replication in vero cells
    Keivan Zandi
    Tropical Infectious Diseases Research and Education Center TIDREC, Department of Medical Microbiology, Faculty of Medicine, University Malaya, Kuala Lumpur 50603, Malaysia
    Molecules 17:2437-45. 2012
    This study investigates the effects of 2-phenyl-1-benzopyran-4-one (flavone) on DENV-2 infectivity in Vero cells. Virus adsorption and attachment and intracellular virus replication were investigated using a foci forming unit assay (FFUA) ..
  77. pmc Suppression of herpes simplex virus 1 in MDBK cells via the interferon pathway
    Cristina Barreca
    Marie Curie Research Institute, Oxted, The Chart, Surrey RH8 0TL, United Kingdom
    J Virol 78:8641-53. 2004
    ..Compared to Vero cells, MDBK cells supported a normal productive infection at a high multiplicity with complete cell destruction...
  78. pmc Phenotype of a herpes simplex virus type 1 mutant that fails to express immediate-early regulatory protein ICP0
    Roger D Everett
    MRC Virology Unit, Institute of Virology, Glasgow G11 5JR, Scotland, United Kingdom
    J Virol 78:1763-74. 2004
    ....
  79. pmc SYBR green-based real-time quantitative PCR assay for detection of West Nile Virus circumvents false-negative results due to strain variability
    James F Papin
    Department of Microbiology and Immunology, The University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73104, USA
    J Clin Microbiol 42:1511-8. 2004
    ....
  80. pmc SLAM (CD150)-independent measles virus entry as revealed by recombinant virus expressing green fluorescent protein
    Koji Hashimoto
    Department of Virology, Faculty of Medicine, Kyushu University, Fukuoka 812 8582, Japan
    J Virol 76:6743-9. 2002
    ..These results indicate that MV can infect a variety of cells, albeit with a low efficiency, by using an as yet unidentified receptor(s) other than SLAM or CD46, in part explaining the observed MV infection of SLAM-negative cells in vivo...
  81. ncbi Bats are natural reservoirs of SARS-like coronaviruses
    Wendong Li
    Institute of Zoology, Chinese Academy of Sciences CAS, Beijing, China
    Science 310:676-9. 2005
    ..The human and civet isolates of SARS-CoV nestle phylogenetically within the spectrum of SL-CoVs, indicating that the virus responsible for the SARS outbreak was a member of this coronavirus group...
  82. pmc Preclinical evaluation of a replication-deficient intranasal DeltaNS1 H5N1 influenza vaccine
    Julia Romanova
    Avir Green Hills Biotechnology AG, Vienna, Austria
    PLoS ONE 4:e5984. 2009
    ..As a result, DeltaNS1 viruses elicit strong B- and T- cell mediated immune responses...
  83. doi One time intranasal vaccination with a modified vaccinia Tiantan strain MVTT(ZCI) protects animals against pathogenic viral challenge
    Wenbo Yu
    AIDS Center and Modern Virology Research Center, State Key Laboratory of Virology, College of Life Sciences, Wuhan University, PR China
    Vaccine 28:2088-96. 2010
    ..This study established proof-of-concept that the attenuated replicating MVTT(ZCI) may serve as a safe noninvasive smallpox vaccine candidate...
  84. pmc Characterization of a novel high-affinity monoclonal immunoglobulin G antibody against the ricin B subunit
    Carolyn R McGuinness
    Division of Infectious Disease, Wadsworth Center, 120 New Scotland Avenue, Albany, NY 12208, USA
    Infect Immun 74:3463-70. 2006
    ..Finally, we demonstrate that R70 and 24B11, when combined, function synergistically to neutralize ricin in vitro, raising the possibility that these two MAbs could serve as a novel immunotherapeutic in vivo...
  85. ncbi Acetone, ethanol and methanol extracts of Phyllanthus urinaria inhibit HSV-2 infection in vitro
    Chien Min Yang
    Graduate Institute of Pharmaceutical Sciences, College of Pharmacy, No 100, Shin Chuan 1 st Road, Kaohsiung Medical University, 807 Kaohsiung, Taiwan, ROC
    Antiviral Res 67:24-30. 2005
    ..5, 5.0 +/ -0.4 and 4.0 +/- 0.9 mcg/ml, respectively. All three extracts showed no cytotoxic effect against Vero cells at concentrations of 10.0 mcg/ml or below...
  86. pmc Anti-heparan sulfate peptides that block herpes simplex virus infection in vivo
    Vaibhav Tiwari
    Departments of Ophthalmology and Visual Sciences, University of Illinois, Chicago, Illinois 60612, USA
    J Biol Chem 286:25406-15. 2011
    ..This result also highlights an in vivo significance of HS and 3-OS HS during ocular herpes infection...
  87. doi Interaction of dengue virus envelope protein with endoplasmic reticulum-resident chaperones facilitates dengue virus production
    Thawornchai Limjindaporn
    Department of Anatomy, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand
    Biochem Biophys Res Commun 379:196-200. 2009
    ..These results indicate that the interaction of these three chaperones with DENV E plays an important role in virion production, likely facilitating proper folding and assembly of dengue proteins...
  88. pmc Regulation of host translational machinery by African swine fever virus
    Alfredo Castello
    Centro de Biologia Molecular Severo Ochoa, CSIC UAM, Universidad Autonoma de Madrid, Madrid, Spain
    PLoS Pathog 5:e1000562. 2009
    ..are indispensable for ASFV protein synthesis and virus spread, since eIF4E or eIF4G depletion in COS-7 or Vero cells strongly prevents accumulation of viral proteins and decreases virus titre...
  89. pmc Inhibition of Nipah virus infection in vivo: targeting an early stage of paramyxovirus fusion activation during viral entry
    Matteo Porotto
    Departments of Pediatrics and of Microbiology and Immunology, Weill Medical College of Cornell University, New York, New York, United States of America
    PLoS Pathog 6:e1001168. 2010
    ..The in vivo efficacy of cholesterol-tagged peptides, and in particular their ability to penetrate the CNS, suggests that they are promising candidates for the prevention or therapy of infection by Nipah and other lethal paramyxoviruses...
  90. pmc During lytic infections, herpes simplex virus type 1 DNA is in complexes with the properties of unstable nucleosomes
    Jonathan J Lacasse
    Signal Transduction Research Group, Molecular Mechanisms of Growth Control Research Group, University of Alberta, 327C Heritage Medical Research Center, Edmonton, Alberta T6G 2S2, Canada
    J Virol 84:1920-33. 2010
    ..The HSV-1 DNAs in complexes fractionating as mono- to dinucleosomes were stabilized by cross-linking. Therefore, most HSV-1 DNA forms particularly unstable nucleosome-like complexes at 5 h of lytic infection...
  91. pmc Disruption of nuclear organization during the initial phase of African swine fever virus infection
    Maria Ballester
    Centre de Recerca en Sanitat Animal CReSA, UAB IRTA, Campus de la Universitat Autonoma de Barcelona, Barcelona, 08193, Spain
    J Virol 85:8263-9. 2011
    ....
  92. pmc Rab9 GTPase is required for replication of human immunodeficiency virus type 1, filoviruses, and measles virus
    James L Murray
    National Center for HIV, STD, and TB Prevention, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    J Virol 79:11742-51. 2005
    ..These results suggest that Rab9 is an important cellular target for inhibiting diverse viruses and help to define a late-endosome-to-plasma-membrane vesicular transport pathway important in viral assembly...
  93. ncbi Continuous cell lines as a production system for influenza vaccines
    Yvonne Genzel
    Max Planck Institute for Dynamics of Complex Technical Systems, Bioprocess Engineering Magdeburg, Sandtorstr 1, Magdeburg 39106, Germany
    Expert Rev Vaccines 8:1681-92. 2009
    ..These encouraging developments for cell culture-based influenza virus production are summarized and an overview on potential cell substrates is given...
  94. pmc Persistent infection with ebola virus under conditions of partial immunity
    Manisha Gupta
    Special Pathogens Branch, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    J Virol 78:958-67. 2004
    ....
  95. pmc A novel function of heparan sulfate in the regulation of cell-cell fusion
    Christopher D O'Donnell
    Department of Ophthalmology and Visual Sciences and the Department of Microbiology and Immunology, College of Medicine, University of Illinois, Chicago, Illinois 60612, USA
    J Biol Chem 284:29654-65. 2009
    ..This could serve as a possible explanation as to why HS-deficient cells showed significantly enhanced cell-cell fusion and suggests that HS could regulate fusion via fine tuning of RhoA and Cdc42 activities...
  96. pmc Dengue virus infection induces upregulation of GRP78, which acts to chaperone viral antigen production
    S Wati
    Infectious Diseases Laboratories, SA Pathology, Frome Road, Adelaide, South Australia 5000
    J Virol 83:12871-80. 2009
    ..This may be a common requirement for viruses such as flaviviruses that depend heavily on the ER for coordinated protein production and processing...
  97. pmc NSm and 78-kilodalton proteins of Rift Valley fever virus are nonessential for viral replication in cell culture
    Sungyong Won
    Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555 1019, USA
    J Virol 80:8274-8. 2006
    ..A competition-propagation assay revealed that the parental virus was slightly more fit than the mutant virus lacking expression of both proteins...
  98. pmc Recruitment of cellular recombination and repair proteins to sites of herpes simplex virus type 1 DNA replication is dependent on the composition of viral proteins within prereplicative sites and correlates with the induction of the DNA damage response
    Dianna E Wilkinson
    Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, Connecticut 06030, USA
    J Virol 78:4783-96. 2004
    ....
  99. ncbi SARS coronavirus, but not human coronavirus NL63, utilizes cathepsin L to infect ACE2-expressing cells
    I Chueh Huang
    Pulmonary Division, Children s Hospital, Children s Hospital Laboratory of Molecular Medicine, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 281:3198-203. 2006
    ..Our data indicate that two coronaviruses that utilize a common receptor nonetheless enter cells through distinct mechanisms...
  100. pmc Transfectant influenza A viruses with long deletions in the NS1 protein grow efficiently in Vero cells
    A Egorov
    Institute of Applied Microbiology, University of Agriculture, A 1190 Vienna, Austria
    J Virol 72:6437-41. 1998
    ..Whereas all deletion mutants grew to high titers in Vero cells, growth on Madin-Darby canine kidney (MDCK) cells and replication in mice decreased with increasing length of ..
  101. pmc Quantitative proteomics using stable isotope labeling with amino acids in cell culture reveals changes in the cytoplasmic, nuclear, and nucleolar proteomes in Vero cells infected with the coronavirus infectious bronchitis virus
    Edward Emmott
    Institute of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds, Leeds, United Kingdom
    Mol Cell Proteomics 9:1920-36. 2010
    ..Here we detail a high throughput quantitative proteomics analysis of Vero cells infected with the coronavirus infectious bronchitis virus (IBV), a positive strand RNA virus that replicates in ..

Research Grants69

  1. HTS assay and configuration for inhibitors of the UDP-Mur formation pathway
    Michael McNeil; Fiscal Year: 2009
    ..We will also follow up by performing initial toxicity testing against Vero cells. Further development will focus on development of hits to drugs against tuberculosis as this is our lab's ..
  2. A SARS-CoV Spike Protein Vaccine
    B Sim; Fiscal Year: 2004
    ..Anti-sera will be assessed for antibodies to spike protein by ELISA and capacity to neutralize virus invasion of Vero cells in vitro...
  3. A SARS-CoV Spike Protein Vaccine
    B Sim; Fiscal Year: 2005
    ..Anti-sera will be assessed for antibodies to spike protein by ELISA and capacity to neutralize virus invasion of Vero cells in vitro...
  4. GATING AND TRANSLOCATION IN THE DIPHTHERIA TOXIN CHANNEL
    Bruce Kagan; Fiscal Year: 1992
    ..Patch clamp experiments will attempt to record DT channels in Vero cells as they cross the cell membrane...
  5. GATING AND TRANSLOCATION IN THE DIPHTHERIA TOXIN CHANNEL
    Bruce Kagan; Fiscal Year: 1990
    ..Patch clamp experiments will attempt to record DT channels in Vero cells as they cross the cell membrane...
  6. Characterization of a novel rabbit herpes simplexvirus: a possible model for unde
    Ling Jin; Fiscal Year: 2009
    ..the skin of infected rabbits produced syncytia and cell lysis in cultured rabbit skin and kidney cells, and in Vero cells. PCR using degenerative primers to conserved herpesvirus genes was used to amplify sequences from purified viral ..
  7. Characterization of a novel rabbit herpes simplexvirus: a possible model for unde
    LING J JIN; Fiscal Year: 2010
    ..the skin of infected rabbits produced syncytia and cell lysis in cultured rabbit skin and kidney cells, and in Vero cells. PCR using degenerative primers to conserved herpesvirus genes was used to amplify sequences from purified viral ..
  8. Developing a Paramyxovirus-based H5N1 Vaccine
    Biao He; Fiscal Year: 2010
    ..PIV5 can be produced in high titers (up to 8x108 plaque forming units (PFU)/ml) in many cells including Vero cells, which are WHO approved for vaccine production. PIV5 can infect human cell lines as well as primary human cells...
  9. Developing a Paramyxovirus-based H5N1 Vaccine
    Biao He; Fiscal Year: 2009
    ..PIV5 can be produced in high titers (up to 8x108 plaque forming units (PFU)/ml) in many cells including Vero cells, which are WHO approved for vaccine production. PIV5 can infect human cell lines as well as primary human cells...
  10. Chimeric Alphavirus Vaccines for Chikungunya
    Scott Weaver; Fiscal Year: 2009
    ..and structural protein genes from CHIKV, we will optimize vaccine candidates that replicate efficiently in Vero cells, and are highly attenuated yet rapidly immunogenic and efficacious in preventing viremia and disease...
  11. Chimeric Alphavirus Vaccines for Chikungunya
    Scott C Weaver; Fiscal Year: 2010
    ..and structural protein genes from CHIKV, we will optimize vaccine candidates that replicate efficiently in Vero cells, and are highly attenuated yet rapidly immunogenic and efficacious in preventing viremia and disease...
  12. Discovery and Optimization of Novel Integrase Inhibitors as Anti-HIV Agents
    John Buolamwini; Fiscal Year: 2009
    ..Toxicity of compounds will be tested using uninfected PBMCs as well as CEM leukemia and Vero cells to assess therapeutic index...
  13. CHARACTERIZATION OF HCMV UL84
    Gregory Pari; Fiscal Year: 2009
    ..In Vero cells, IE2 apparently represses a high basal level of activation from this promoter...
  14. Discovery and Optimization of Novel Integrase Inhibitors as Anti-HIV Agents
    John K Buolamwini; Fiscal Year: 2010
    ..Toxicity of compounds will be tested using uninfected PBMCs as well as CEM leukemia and Vero cells to assess therapeutic index...
  15. CHARACTERIZATION OF HCMV UL84
    Gregory Pari; Fiscal Year: 2007
    ..In Vero cells, IE2 apparently represses a high basal level of activation from this promoter...
  16. CHARACTERIZATION OF HCMV UL84
    Gregory Pari; Fiscal Year: 2006
    ..In Vero cells, IE2 apparently represses a high basal level of activation from this promoter...
  17. CHARACTERIZATION OF HCMV UL84
    Gregory Pari; Fiscal Year: 2005
    ..In Vero cells, IE2 apparently represses a high basal level of activation from this promoter...
  18. ACTIN BASED MOTILITY BY RICKETTSIA RICKETTSII
    ROBERT HEINZEN; Fiscal Year: 2002
    ..Transfected Vero cells producing specific domains of rOmpA or rOmpB, and infected with R...
  19. GLYCOLIPIDS AND HEMOLYTIC UREMIC SYNDROME
    David Haslam; Fiscal Year: 2001
    ..In preliminary studies VT-susceptible Vero cells were transfected with the cDNA encoding Forssman synthetase (FS)...
  20. GLYCOLIPIDS AND HEMOLYTIC UREMIC SYNDROME
    David Haslam; Fiscal Year: 2000
    ..In preliminary studies VT-susceptible Vero cells were transfected with the cDNA encoding Forssman synthetase (FS)...
  21. GLYCOLIPIDS AND HEMOLYTIC UREMIC SYNDROME
    David Haslam; Fiscal Year: 2002
    ..In preliminary studies VT-susceptible Vero cells were transfected with the cDNA encoding Forssman synthetase (FS)...
  22. GLYCOLIPIDS AND HEMOLYTIC UREMIC SYNDROME
    David Haslam; Fiscal Year: 1999
    ..In preliminary studies VT-susceptible Vero cells were transfected with the cDNA encoding Forssman synthetase (FS)...
  23. Development of a Novel, Chimeric Chikungunya Vaccine
    Richard Kinney; Fiscal Year: 2009
    ..generate and optimize chimeric cDNA clones to produce candidate vaccine strains that replicate efficiently in Vero cells, and are highly attenuated yet rapidly immunogenic and efficacious in preventing CHIK disease...
  24. Development of a Novel, Chimeric Chikungunya Vaccine
    Richard Kinney; Fiscal Year: 2009
    ..generate and optimize chimeric cDNA clones to produce candidate vaccine strains that replicate efficiently in Vero cells, and are highly attenuated yet rapidly immunogenic and efficacious in preventing CHIK disease...
  25. ACTIN BASED MOTILITY BY RICKETTSIA RICKETTSII
    ROBERT HEINZEN; Fiscal Year: 2000
    ..Transfected Vero cells producing specific domains of rOmpA or rOmpB, and infected with R...
  26. ACTIN BASED MOTILITY BY RICKETTSIA RICKETTSII
    ROBERT HEINZEN; Fiscal Year: 2001
    ..Transfected Vero cells producing specific domains of rOmpA or rOmpB, and infected with R...
  27. ACTIN BASED MOTILITY BY RICKETTSIA RICKETTSII
    ROBERT HEINZEN; Fiscal Year: 1999
    ..Transfected Vero cells producing specific domains of rOmpA or rOmpB, and infected with R...
  28. STUDIES ON DIPHTHERIA TOXIN AND ITS RECEPTOR
    Leon Eidels; Fiscal Year: 1990
    ..fully and purify the specific diphtheria toxin-binding cell surface protein(s) from highly toxin-sensitive Vero cells. The diphtheria toxin-binding proteins are detected by i) prebinding toxin to extrinsically radiolabeled Vero ..
  29. CHIMERIC YF/DEN2 AS A VACCINE CANDIDATE FOR DENGUE VIRUS
    Farshad Guirakhoo; Fiscal Year: 1999
    ..Viruses were recovered upon transfection of Vero cells with mRNA and amplified twice in these cells to yield a titer of 6.3 log(10p)fu/ml...
  30. CYTOMEGALOVIRUS DNA REPLICATION AND RECOMBINATION
    Edward Mocarski; Fiscal Year: 1993
    ..will be assayed by transfection of CMV-permissive human fibroblast and U373-MG cells as well as nonpermissive Vero cells and COS-7 cells, a cell line which will produce high levels of each of the products...
  31. CYTOMEGALOVIRUS DNA REPLICATION AND RECOMBINATION
    Edward Mocarski; Fiscal Year: 1992
    ..will be assayed by transfection of CMV-permissive human fibroblast and U373-MG cells as well as nonpermissive Vero cells and COS-7 cells, a cell line which will produce high levels of each of the products...
  32. Ab Alphavirus Replicon Vaccine against Cytomegalovirus
    Robert Esch; Fiscal Year: 2005
    ..Purified RNA transcripts produced from the replicon and helper plasmids will be electroporated into certified Vero cells using various amounts and electroporation parameters to identify the optimal conditions for transfection ..
  33. Ab Alphavirus Replicon Vaccine against Cytomegalovirus
    Robert Esch; Fiscal Year: 2004
    ..Purified RNA transcripts produced from the replicon and helper plasmids will be electroporated into certified Vero cells using various amounts and electroporation parameters to identify the optimal conditions for transfection ..
  34. NATIONAL COOPERATIVE DRUG DISCOVERY GROUP/AIDS
    David Boykin; Fiscal Year: 1991
    ..HIV-1 (strain LAV in PBMC) of less than 0.1 (mu)M and therapeutic ratios of greater than 100 in both PBMC and Vero cells. In addition, we have found approximately 10 other compounds with EC50(s) of less than 1 (mu)M and similar ..
  35. DIPHTHERIA TOXIN AND ITS RECEPTOR
    Leon Eidels; Fiscal Year: 1992
    ..to characterize fully the specific diphtheria toxin-binding cell surface protein(s) from highly toxin-sensitive Vero cells that function as the toxin receptor...
  36. DIPHTHERIA TOXIN AND ITS RECEPTOR
    Leon Eidels; Fiscal Year: 1993
    ..to characterize fully the specific diphtheria toxin-binding cell surface protein(s) from highly toxin-sensitive Vero cells that function as the toxin receptor...
  37. Microcarrier culture system for human influenza vaccine
    WILLIAM HILLEGAS; Fiscal Year: 2003
    ..integrate critical cell culture and viral growth parameters for optimal production of human influenza virus in Vero cells grown in Hillex microcarrier-suspension culture. Specific Aim II...
  38. Microcarrier culture system for human influenza vaccine
    WILLIAM HILLEGAS; Fiscal Year: 2004
    ..integrate critical cell culture and viral growth parameters for optimal production of human influenza virus in Vero cells grown in Hillex microcarrier-suspension culture. Specific Aim II...
  39. FUSION DOMAINS OF HSV-1 PROTEINS
    KONSTANTIN KOUSOULAS; Fiscal Year: 1990
    ..factor(FF), and FF(Syn1) will be cloned into SV40 transient expression vectors and co-expressed in COS cells and Vero cells to define different combinations of these proteins that cause cell fusion...
  40. Recombinant Human Serum Albumin for Use in Cell Culture
    Ning Huang; Fiscal Year: 2010
    ..extend the studies in phase I to test the effect of rHSA on cell growth and productivity of hybridoma, CHO and Vero cells in larger scale cell culture with commercial media...
  41. ANTIFOLATES AGAINST MYCOBACTERIAL INFECTIONS IN AIDS
    WILLIAM BARROW; Fiscal Year: 1999
    ..tuberculosis H37Rv, and low toxicity to Vero cells. At SRI, the derivatives have shown selective inhibition in a cell-free DHFR assay from M...
  42. Recombinant Human Serum Albumin for Use in Cell Culture
    Ning Huang; Fiscal Year: 2009
    ..extend the studies in phase I to test the effect of rHSA on cell growth and productivity of hybridoma, CHO and Vero cells in larger scale cell culture with commercial media...
  43. STRUCTURE AND ACTION OF PLANT DEFENSIVE PROTEINS
    Jon Robertus; Fiscal Year: 1993
    ..We have developed and tested a translocation assay system using cultured Vero cells. For our analysis, mutants will be made in potential membrane recognition sequences of RTA...
  44. NS1 PROTEIN IN INTERFERON INDUCTION & VIRUS REPLICATION
    John Treanor; Fiscal Year: 2002
    ..induction of type I interferons, and 4) normal levels of replication in interferon-deficient systems, such as Vero cells, 6-day old eggs, on STAT -/- MICE...
  45. NS1 PROTEIN IN INTERFERON INDUCTION & VIRUS REPLICATION
    John Treanor; Fiscal Year: 2000
    ..induction of type I interferons, and 4) normal levels of replication in interferon-deficient systems, such as Vero cells, 6-day old eggs, on STAT -/- MICE...
  46. NS1 PROTEIN IN INTERFERON INDUCTION & VIRUS REPLICATION
    John Treanor; Fiscal Year: 2001
    ..induction of type I interferons, and 4) normal levels of replication in interferon-deficient systems, such as Vero cells, 6-day old eggs, on STAT -/- MICE...
  47. ACTIVATION OF BACTERIAL TOXINS IN SUDDEN INFANT DEATH
    James Lindsay; Fiscal Year: 1990
    ..of different ages by examining changes in time to death, alterations to ileal tissue in vivo, and alterations to Vero cells in vitro. 3. To examine the incidence of C...
  48. GOSSYPOL DERIVATIVES AS COVALENT CARRIERS OF ANTIVIRALS
    Robert Royer; Fiscal Year: 1990
    ..In vitro studies of these new drugs will be carried out with HIV, using human T cells; with HSV-II, using Vero cells; and with influenza virus, using MDCK cells...
  49. HIV-LIKE PARTICLES--PRODUCTION AND PURIFICATION SCALE-UP
    STEVEN DISCIULLO; Fiscal Year: 1992
    ..HIV-like particles will be expressed in large-scale microcarrier cultures of Vero cells infected with the recombinant virus...
  50. HIV-LIKE PARTICLES: PRODUCTION AND PURIFICATION SCALE-UP
    STEVEN DISCIULLO; Fiscal Year: 1993
    ..HIV-like particles will be expressed in large-scale microcarrier cultures of Vero cells infected with the recombinant virus...
  51. CELLULAR IMMUNITY IN SICK AND MALNOURISHED INFANTS
    E Stiehm; Fiscal Year: 1990
    ..EVR is measured by a quantitative plaque reduction assay utilizing herpes simplex virus type I in Vero cells as well as assays of viral release into supernatants from infected CEM cell supernatants...
  52. CONTROL OF HIV1 AND HSV-1 INFECTION WITH PLANT LIGNANS
    RU CHIH HUANG; Fiscal Year: 1999
    ..4 and / or related plant lignans can suppress wild type HSV-1 and recombinant HSV ICP4LACZ in African Green Monkey (VERO) cells,(3) to study the effect of plant lignan in controlling HSV 1 in infected Hu-PBL-SCID mice. ..
  53. CONTROL OF HIV-1 AND HSV-1 INFECTION WITH PLANT LIGNANS
    RU CHIH HUANG; Fiscal Year: 2000
    ..4 and / or related plant lignans can suppress wild type HSV-1 and recombinant HSV ICP4LACZ in African Green Monkey (VERO) cells,(3) to study the effect of plant lignan in controlling HSV 1 in infected Hu-PBL-SCID mice. ..
  54. CONTROL OF HIV-1 AND HSV-1 INFECTION WITH PLANT LIGNANS
    RU CHIH HUANG; Fiscal Year: 2000
    ..4 and / or related plant lignans can suppress wild type HSV-1 and recombinant HSV ICP4LACZ in African Green Monkey (VERO) cells,(3) to study the effect of plant lignan in controlling HSV 1 in infected Hu-PBL-SCID mice. ..
  55. POSTREPLICATION REPAIR IN CULTURED MAMMALIAN CELLS
    GEORGE BOWDEN; Fiscal Year: 1980
    ..The proposed studies make use of primary cultures of mouse epidermal cells and Vero cells (an African green monkey kidney cell line)...
  56. CONTROL OF HIV-1 AND HSV-1 INFECTION WITH PLANT LIGNANS
    RU CHIH HUANG; Fiscal Year: 1999
    ..4 and / or related plant lignans can suppress wild type HSV-1 and recombinant HSV ICP4LACZ in African Green Monkey (VERO) cells,(3) to study the effect of plant lignan in controlling HSV 1 in infected Hu-PBL-SCID mice. ..
  57. REPLICATION OF HIV IN BRAIN CELL CULTURES
    Paul Shapshak; Fiscal Year: 1991
    ..can infect and replicate in human neuroblastoma cells, but not in human astrocytoma and Hela cells nor in monkey Vero cells. Comparisons of radioactively labeled viral proteins in brain, lymphocytes, and neuroblastoma cells will be made ..