keratinocytes

Summary

Summary: Epidermal cells which synthesize keratin and undergo characteristic changes as they move upward from the basal layers of the epidermis to the cornified (horny) layer of the skin. Successive stages of differentiation of the keratinocytes forming the epidermal layers are basal cell, spinous or prickle cell, and the granular cell.

Top Publications

  1. ncbi Wound healing--aiming for perfect skin regeneration
    P Martin
    Department of Anatomy and Developmental Biology, University College London, Gower Street, London WC1E 6BT, UK
    Science 276:75-81. 1997
  2. ncbi Cellular motility driven by assembly and disassembly of actin filaments
    Thomas D Pollard
    Department of Cellular, Molecular, and Developmental Biology, Yale University, New Haven, CT 06520, USA
    Cell 112:453-65. 2003
  3. doi Integrative responses to IL-17 and TNF-α in human keratinocytes account for key inflammatory pathogenic circuits in psoriasis
    Andrea Chiricozzi
    Laboratory for Investigative Dermatology, The Rockefeller University, New York, New York, USA
    J Invest Dermatol 131:677-87. 2011
  4. ncbi Keratinocyte-fibroblast interactions in wound healing
    Sabine Werner
    Institute of Cell Biology, Eidgenossische Technische Hochschule Zurich, Honggerberg, Zurich, Switzerland
    J Invest Dermatol 127:998-1008. 2007
  5. ncbi IL-22 regulates the expression of genes responsible for antimicrobial defense, cellular differentiation, and mobility in keratinocytes: a potential role in psoriasis
    Kerstin Wolk
    Interdisciplinary Group of Molecular Immunopathology, Dermatology Medical Immunology, University Hospital Charite, Berlin, Germany
    Eur J Immunol 36:1309-23. 2006
  6. ncbi Endogenous antimicrobial peptides and skin infections in atopic dermatitis
    Peck Y Ong
    Division of Allergy and Immunology, Department of Pediatrics, National Jewish Medical and Research Center, Denver, CO 80206, USA
    N Engl J Med 347:1151-60. 2002
  7. pmc Th17 cytokines interleukin (IL)-17 and IL-22 modulate distinct inflammatory and keratinocyte-response pathways
    K E Nograles
    Laboratory of Investigative Dermatology, The Rockefeller University, 1230 York Avenue, New York, NY 10065, USA
    Br J Dermatol 159:1092-102. 2008
  8. pmc Genome-wide profiling of p63 DNA-binding sites identifies an element that regulates gene expression during limb development in the 7q21 SHFM1 locus
    Evelyn N Kouwenhoven
    Department of Human Genetics, Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
    PLoS Genet 6:e1001065. 2010
  9. pmc p63-microRNA feedback in keratinocyte senescence
    Pia Rivetti di Val Cervo
    Istituto Dermopatico dell Immacolata Istituto di Ricovero e Cura a Carattere Scientifico, Laboratory of Biochemistry, Department of Experimental Medicine and Biochemical Sciences, and University of Rome Tor Vergata, 00133 Rome, Italy
    Proc Natl Acad Sci U S A 109:1133-8. 2012
  10. pmc RhoA is dispensable for skin development, but crucial for contraction and directed migration of keratinocytes
    Ben Jackson
    Biomedical Institute, BRIC, University of Copenhagen, 2200 Copenhagen, Denmark
    Mol Biol Cell 22:593-605. 2011

Research Grants

  1. Role of CRH in skin physiology and pathology
    Andrzej Slominski; Fiscal Year: 2004
  2. CHARACTERIZATION OF NEUROPILIN, A NOVEL VEGF RECEPTOR
    Michael Klagsbrun; Fiscal Year: 2007
  3. Somatic Stem Cells in Engineered Human Skin
    Steven Boyce; Fiscal Year: 2009
  4. Role of OCT4 in reprogramming human skin keratinocytes
    Jackie R Bickenbach; Fiscal Year: 2010
  5. Plexin signalling in melanocyte biology and melanoma progression
    Glynis A Scott; Fiscal Year: 2010
  6. The Role of bacterial toxins in human skin disease.
    DONALD YM contact LEUNG; Fiscal Year: 2010
  7. Somatic Stem Cells in Engineered Human Skin
    Steven Boyce; Fiscal Year: 2009
  8. THE IDENTITY OF THE LATENT NEOPLASTIC EPIDERMAL CELL
    Rebecca Morris; Fiscal Year: 1993
  9. Trim32 Regulation of Piasy in Skin Homeostasis
    Molly Kulesz Martin; Fiscal Year: 2009
  10. Trim32 Regulation of Piasy in Skin Homeostasis
    Molly F Kulesz Martin; Fiscal Year: 2010

Detail Information

Publications345 found, 100 shown here

  1. ncbi Wound healing--aiming for perfect skin regeneration
    P Martin
    Department of Anatomy and Developmental Biology, University College London, Gower Street, London WC1E 6BT, UK
    Science 276:75-81. 1997
    ....
  2. ncbi Cellular motility driven by assembly and disassembly of actin filaments
    Thomas D Pollard
    Department of Cellular, Molecular, and Developmental Biology, Yale University, New Haven, CT 06520, USA
    Cell 112:453-65. 2003
    ..Profilin catalyzes the exchange of ADP for ATP, refilling the pool of ATP-actin monomers bound to profilin, ready for elongation...
  3. doi Integrative responses to IL-17 and TNF-α in human keratinocytes account for key inflammatory pathogenic circuits in psoriasis
    Andrea Chiricozzi
    Laboratory for Investigative Dermatology, The Rockefeller University, New York, New York, USA
    J Invest Dermatol 131:677-87. 2011
    ..Our data predict that psoriasis therapy with either TNF or IL-17 antagonists will produce greater modulation of the synergistic/additive gene set, which consists of the most highly expressed genes in psoriasis skin lesions...
  4. ncbi Keratinocyte-fibroblast interactions in wound healing
    Sabine Werner
    Institute of Cell Biology, Eidgenossische Technische Hochschule Zurich, Honggerberg, Zurich, Switzerland
    J Invest Dermatol 127:998-1008. 2007
    ..There is ample evidence that keratinocytes stimulate fibroblasts to synthesize growth factors, which in turn will stimulate keratinocyte proliferation in ..
  5. ncbi IL-22 regulates the expression of genes responsible for antimicrobial defense, cellular differentiation, and mobility in keratinocytes: a potential role in psoriasis
    Kerstin Wolk
    Interdisciplinary Group of Molecular Immunopathology, Dermatology Medical Immunology, University Hospital Charite, Berlin, Germany
    Eur J Immunol 36:1309-23. 2006
    ..demonstrate that IL-22, in contrast to its relative IFN-gamma, regulates the expression of only a few genes in keratinocytes. This is due to varied signal transduction...
  6. ncbi Endogenous antimicrobial peptides and skin infections in atopic dermatitis
    Peck Y Ong
    Division of Allergy and Immunology, Department of Pediatrics, National Jewish Medical and Research Center, Denver, CO 80206, USA
    N Engl J Med 347:1151-60. 2002
    ..We compared the levels of expression of LL-37 and human beta-defensin 2 (HBD-2) in inflamed skin from patients with atopic dermatitis and from those with psoriasis...
  7. pmc Th17 cytokines interleukin (IL)-17 and IL-22 modulate distinct inflammatory and keratinocyte-response pathways
    K E Nograles
    Laboratory of Investigative Dermatology, The Rockefeller University, 1230 York Avenue, New York, NY 10065, USA
    Br J Dermatol 159:1092-102. 2008
    ..Psoriasis vulgaris is an inflammatory skin disease mediated by Th1 and Th17 cytokines, yet the relative contribution of interferon (IFN)-gamma, interleukin (IL)-17 and IL-22 on disease pathogenesis is still unknown...
  8. pmc Genome-wide profiling of p63 DNA-binding sites identifies an element that regulates gene expression during limb development in the 7q21 SHFM1 locus
    Evelyn N Kouwenhoven
    Department of Human Genetics, Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
    PLoS Genet 6:e1001065. 2010
    ..profiling by chromatin immunoprecipitation (ChIP), followed by deep sequencing (ChIP-seq) in primary human keratinocytes, and identified potential target genes and regulatory elements controlled by p63...
  9. pmc p63-microRNA feedback in keratinocyte senescence
    Pia Rivetti di Val Cervo
    Istituto Dermopatico dell Immacolata Istituto di Ricovero e Cura a Carattere Scientifico, Laboratory of Biochemistry, Department of Experimental Medicine and Biochemical Sciences, and University of Rome Tor Vergata, 00133 Rome, Italy
    Proc Natl Acad Sci U S A 109:1133-8. 2012
    We investigated the expression of microRNAs (miRNAs) associated with replicative senescence in human primary keratinocytes. A cohort of miRNAs up-regulated in senescence was identified by genome-wide miRNA profiling, and their change in ..
  10. pmc RhoA is dispensable for skin development, but crucial for contraction and directed migration of keratinocytes
    Ben Jackson
    Biomedical Institute, BRIC, University of Copenhagen, 2200 Copenhagen, Denmark
    Mol Biol Cell 22:593-605. 2011
    ..Primary RhoA-null keratinocytes, however, displayed an increased percentage of multinucleated cells, defective maturation of cell-cell ..
  11. ncbi p15INK4B is a potential effector of TGF-beta-induced cell cycle arrest
    G J Hannon
    Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, New York 11724
    Nature 371:257-61. 1994
    ..p15 expression is induced approximately 30-fold in human keratinocytes by treatment with TGF-beta, suggesting that p15 may act as an effector of TGF-beta-mediated cell cycle arrest...
  12. pmc p63 regulates proliferation and differentiation of developmentally mature keratinocytes
    Amy B Truong
    VA Palo Alto Health Care System, Palo Alto, California 94304, USA
    Genes Dev 20:3185-97. 2006
    ..These data indicate that p63 is required for both the proliferative and differentiation potential of developmentally mature keratinocytes.
  13. pmc p63 is the molecular switch for initiation of an epithelial stratification program
    Maranke I Koster
    Program in Developmental Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Genes Dev 18:126-31. 2004
    ..Our data demonstrate that p63 plays a dual role: initiating epithelial stratification during development and maintaining proliferative potential of basal keratinocytes in mature epidermis.
  14. ncbi Inflammation in wound repair: molecular and cellular mechanisms
    Sabine A Eming
    Department of Dermatology, University of Cologne, Cologne, Germany
    J Invest Dermatol 127:514-25. 2007
    ..In this paper, we review cellular and molecular mechanisms controlling inflammation in cutaneous tissue repair and provide a rationale for targeting the inflammatory phase in order to modulate the outcome of the healing response...
  15. pmc Desmosome disassembly in response to pemphigus vulgaris IgG occurs in distinct phases and can be reversed by expression of exogenous Dsg3
    Jean M Jennings
    Departments of Cell Biology and Dermatology, Emory University, Atlanta, Georgia, USA
    J Invest Dermatol 131:706-18. 2011
    ..The mechanism by which PV IgG disrupts adhesion is not fully understood. To address this issue, primary human keratinocytes (KCs) and patient IgG were used to define the morphological, biochemical, and functional changes triggered by ..
  16. ncbi Perp is a p63-regulated gene essential for epithelial integrity
    Rebecca A Ihrie
    Division of Radiation and Cancer Biology, Stanford University School of Medicine, Stanford, California 94305, USA
    Cell 120:843-56. 2005
    ..These findings demonstrate that Perp is a key effector in the p63 developmental program, playing an essential role in an adhesion subprogram central to epithelial integrity and homeostasis...
  17. pmc Evidence for alteration of EZH2, BMI1, and KDM6A and epigenetic reprogramming in human papillomavirus type 16 E6/E7-expressing keratinocytes
    Paula L Hyland
    Centre for Cancer Research and Cell Biology, School of Medicine, Dentistry and Biomedical Sciences, Queen s University Belfast, 97 Lisburn Road, Belfast BT9 7BL, United Kingdom
    J Virol 85:10999-1006. 2011
    ..We report here that transformed HPV16 E6/E7-expressing primary human foreskin keratinocytes (HFKs) (E6/E7 cells) demonstrate increased expression of the PRC2 methyltransferase EZH2 at both the mRNA and ..
  18. ncbi IL-22 inhibits epidermal differentiation and induces proinflammatory gene expression and migration of human keratinocytes
    Katia Boniface
    Laboratoire Cytokines et Inflammation, UPRES EA 3806, Pole Biologie Sante, Universite de Poitiers, Poitiers, France
    J Immunol 174:3695-702. 2005
    ..In the present study, we show that short-term cultured human epidermal keratinocytes express a functional IL-22R but no IL-10R...
  19. doi Unique and redundant functions of integrins in the epidermis
    Coert Margadant
    Division of Cell Biology, The Netherlands Cancer Institute, Amsterdam, The Netherlands
    FASEB J 24:4133-52. 2010
    ..Furthermore, we discuss human pathologies associated with altered integrin functions in the epidermis...
  20. pmc Global gene expression analysis reveals evidence for decreased lipid biosynthesis and increased innate immunity in uninvolved psoriatic skin
    Johann E Gudjonsson
    Department of Dermatology, University of Michigan, 1910 Taubman Center, Ann Arbor, Michigan 48109, USA
    J Invest Dermatol 129:2795-804. 2009
    ..Taken together, our results identify a "pre-psoriatic" gene expression signature, suggesting decreased lipid biosynthesis and increased innate immunity in uninvolved psoriatic skin...
  21. pmc Ultraviolet irradiation-induces epidermal growth factor receptor (EGFR) nuclear translocation in human keratinocytes
    Yiru Xu
    Department of Dermatology, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA
    J Cell Biochem 107:873-80. 2009
    ..role in mediating ultraviolet (UV) irradiation-induced signal transduction and gene expression in human keratinocytes. EGFR activation results from increased phosphorylation on specific tyrosine residues in the C-terminal ..
  22. pmc Dual role of COUP-TF-interacting protein 2 in epidermal homeostasis and permeability barrier formation
    Olga Golonzhka
    Department of Biochemistry and Biophysics, Oregon State University, Corvallis, Oregon 97331, USA
    J Invest Dermatol 129:1459-70. 2009
    ..The analysis of conditionally null mice, in which expression of CTIP2 was ablated specifically in epidermal keratinocytes, suggests that CTIP2 functions in both cell and non-cell autonomous contexts to exert regulatory influence ..
  23. ncbi Enrichment for living murine keratinocytes from the hair follicle bulge with the cell surface marker CD34
    Carol S Trempus
    Cancer Biology Group, National Center for Toxicogenomics, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA
    J Invest Dermatol 120:501-11. 2003
    ..the hematopoietic stem and progenitor cell marker, CD34, as a potential marker of hair follicle bulge keratinocytes. Using a CD34-specific antibody, we identified intense membrane staining on keratinocytes in the bulge region ..
  24. pmc Pemphigus vulgaris IgG-induced desmoglein-3 endocytosis and desmosomal disassembly are mediated by a clathrin- and dynamin-independent mechanism
    Emmanuella Delva
    Graduate Program in Biochemistry, Cell, and Developmental Biology, Department of Cell Biology, Emory University, Atlanta, GA 30332, USA
    J Biol Chem 283:18303-13. 2008
    ..To define the endocytic machinery involved in PV IgG-induced Dsg3 internalization, human keratinocytes were incubated with PV IgG, and various tools were used to perturb distinct endocytic pathways. The PV IgG...
  25. pmc Targeted disruption of the LAMA3 gene in mice reveals abnormalities in survival and late stage differentiation of epithelial cells
    M C Ryan
    Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
    J Cell Biol 145:1309-23. 1999
    ..we were able to detect a new ligand for integrin alpha3beta1 in the epidermal BM, suggesting that basal keratinocytes can utilize integrin alpha3beta1 to interact with an alternative ligand...
  26. pmc Synthesis of infectious human papillomavirus type 18 in differentiating epithelium transfected with viral DNA
    C Meyers
    Department of Microbiology and Immunology, The Pennsylvania State University College of Medicine, Hershey 17033, USA
    J Virol 71:7381-6. 1997
    ..We have artificially introduced linearized HPV18 genomic DNA into primary keratinocytes by electroporation, followed by clonal expansion and induction of epithelial stratification and ..
  27. pmc Herpes simplex virus type 1 enters human epidermal keratinocytes, but not neurons, via a pH-dependent endocytic pathway
    Anthony V Nicola
    Medical Virology Section, Laboratory of Clinical Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892 1888, USA
    J Virol 79:7609-16. 2005
    ..of lysosomotropic agents, which raise endosomal pH, blocked HSV entry into primary and transformed human keratinocytes, but not into human neurons or neuroblastoma lines...
  28. pmc A regulatory feedback loop involving p63 and IRF6 links the pathogenesis of 2 genetically different human ectodermal dysplasias
    Francesca Moretti
    Department of Dermatology, University of Rome Tor Vergata, Rome, Italy
    J Clin Invest 120:1570-7. 2010
    ..Here, we used human and mouse primary keratinocytes and mouse models to investigate the role of p63 and IRF6 in proliferation and differentiation...
  29. doi A skin microRNA promotes differentiation by repressing 'stemness'
    Rui Yi
    Howard Hughes Medical Institute, The Rockefeller University, New York City, New York 10065, USA
    Nature 452:225-9. 2008
    ..Our findings suggest that miR-203 defines a molecular boundary between proliferative basal progenitors and terminally differentiating suprabasal cells, ensuring proper identity of neighbouring layers...
  30. pmc Cytokine modulation of atopic dermatitis filaggrin skin expression
    Michael D Howell
    Department of Pediatrics, National Jewish Medical and Research Center, Denver, CO 80206, USA
    J Allergy Clin Immunol 120:150-5. 2007
    ..Atopic dermatitis (AD) is a chronic inflammatory skin disease that is characterized by a defective skin barrier function. Recent studies have reported mutations of the skin barrier gene encoding filaggrin in a subset of patients with AD...
  31. doi Actin and serum response factor transduce physical cues from the microenvironment to regulate epidermal stem cell fate decisions
    John T Connelly
    Wellcome Trust Centre for Stem Cell Research, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QR, UK
    Nat Cell Biol 12:711-8. 2010
    ..On small (20 microm diameter) circular islands, keratinocytes remained rounded, and differentiated at higher frequency than cells that could spread on large (50 microm ..
  32. ncbi IL-22 increases the innate immunity of tissues
    Kerstin Wolk
    Corporate Research Business Area Dermatology, Schering AG, D 13342 Berlin, Germany
    Immunity 21:241-54. 2004
    ..The expression of IL-22 receptor in keratinocytes was upregulated by Interferon-gamma...
  33. pmc Upregulation of FOXM1 induces genomic instability in human epidermal keratinocytes
    Muy Teck Teh
    Centre for Clinical and Diagnostic Oral Sciences, Institute of Dentistry, Barts and the London School of Medicine and Dentistry, Queen Mary University of London, Turner Street, London E1 2AD, UK
    Mol Cancer 9:45. 2010
    ..This indicates that upregulation of FOXM1 may be an early molecular signal required for aberrant cell cycle and cancer initiation...
  34. pmc Polycomb group proteins are key regulators of keratinocyte function
    Richard L Eckert
    Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
    J Invest Dermatol 131:295-301. 2011
    ..These proteins are expressed in progenitor cells, in the basal layer, and in suprabasal keratinocytes, and the level, timing, and distribution of expression suggest that the PcG proteins have a central role in ..
  35. pmc p38 MAPK activation is downstream of the loss of intercellular adhesion in pemphigus vulgaris
    Xuming Mao
    Department of Dermatology, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    J Biol Chem 286:1283-91. 2011
    ..PV mAbs that cause endocytosis of Dsg3 but do not dissociate keratinocytes because of compensatory adhesion by Dsg1 do not activate p38...
  36. pmc Kindlin-1 Is required for RhoGTPase-mediated lamellipodia formation in keratinocytes
    Cristina Has
    Department of Dermatology, University Medical Center Freiburg, Freiburg 79104, Germany
    Am J Pathol 175:1442-52. 2009
    ..with small-interfering RNA, protein, and imaging studies, to uncover new functions for kindlin-1 in keratinocytes and to discern the molecular pathology of KS...
  37. pmc E6 proteins from multiple human betapapillomavirus types degrade Bak and protect keratinocytes from apoptosis after UVB irradiation
    Michael P Underbrink
    Division of Human Biology, Fred Hutchinson Cancer Research Center, Seattle, WA 98109 1024, USA
    J Virol 82:10408-17. 2008
    ..it is unknown if the ability to degrade Bak is limited to certain beta-HPV types or whether E6 expression in keratinocytes affects other proteins important for apoptosis signaling...
  38. pmc Jarid2 regulates mouse epidermal stem cell activation and differentiation
    Stefania Mejetta
    Department of Differentiation and Cancer, Center for Genomic Regulation and UPF, Barcelona, Spain
    EMBO J 30:3635-46. 2011
    ..We conclude that Jarid2 is required for the scheduled proliferation of epidermal stem and progenitor cells necessary to maintain epidermal homeostasis...
  39. pmc Control of differentiation in a self-renewing mammalian tissue by the histone demethylase JMJD3
    George L Sen
    VA Palo Alto Health Care System, Palo Alto, California 94305, USA
    Genes Dev 22:1865-70. 2008
    ..Within epidermal tissue, JMJD3 depletion blocked differentiation, while active JMJD3 dominantly induced it. These results indicate that epigenetic derepression by JMJD3 controls mammalian epidermal differentiation...
  40. pmc Defining the epithelial stem cell niche in skin
    Tudorita Tumbar
    Howard Hughes Medical Institute, Laboratory of Mammalian Cell Biology and Development, Rockefeller University, New York, NY 10021, USA
    Science 303:359-63. 2004
    ..Many of the >100 messenger RNAs preferentially expressed in the niche encode surface receptors and secreted proteins, enabling LRCs to signal and respond to their environment...
  41. doi Isolation and short-term culture of primary keratinocytes, hair follicle populations and dermal cells from newborn mice and keratinocytes from adult mice for in vitro analysis and for grafting to immunodeficient mice
    Ulrike Lichti
    NIH NCI CCR, Laboratory of Cancer Biology and Genetics, In vitro Pathogenesis Section, 37 Convent Drive, Building 37 4068, Bethesda, Maryland 20892 4255, USA
    Nat Protoc 3:799-810. 2008
    Protocols for preparing and culturing primary keratinocytes from newborn and adult mouse epidermis have evolved over the past 35 years...
  42. pmc TRPV3 in keratinocytes transmits temperature information to sensory neurons via ATP
    Sravan Mandadi
    Division of Cell Signaling, Okazaki Institute for Integrative Bioscience, National Institute for Physiological Sciences, Okazaki, Japan
    Pflugers Arch 458:1093-102. 2009
    Transient receptor potential V3 (TRPV3) and TRPV4 are heat-activated cation channels expressed in keratinocytes. It has been proposed that heat-activation of TRPV3 and/or TRPV4 in the skin may release diffusible molecules which would ..
  43. ncbi The expression of the gene coding for the antibacterial peptide LL-37 is induced in human keratinocytes during inflammatory disorders
    M Frohm
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, S 171 77 Stockholm, Sweden
    J Biol Chem 272:15258-63. 1997
    ..By in situ hybridization and immunohistochemistry the transcript and the peptide were located in keratinocytes throughout the epidermis of the inflammatory regions...
  44. doi Knockdown of filaggrin impairs diffusion barrier function and increases UV sensitivity in a human skin model
    Michael Mildner
    Department of Dermatology, Medical University of Vienna, Vienna, Austria
    J Invest Dermatol 130:2286-94. 2010
    ..In addition, our results challenge the role of filaggrin in intermediate filament aggregation and establish a link between filaggrin and endogenous UVB protection...
  45. pmc Physiological factors that regulate skin pigmentation
    Yuji Yamaguchi
    Department of Geriatric and Environmental Dermatology, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan
    Biofactors 35:193-9. 2009
    ..melanosome biogenesis, transport and transfer, melanogenic regulators in melanocytes, and factors derived from keratinocytes, fibroblasts, endothelial cells, hormones, inflammatory cells, and nerves...
  46. ncbi 1,25-Dihydroxyvitamin D3 inhibits ultraviolet B-induced apoptosis, Jun kinase activation, and interleukin-6 production in primary human keratinocytes
    Petra De Haes
    Laboratory for Experimental Medicine and Endocrinology LEGENDO, Gasthuisberg, Katholieke Universiteit Leuven, 3000 Leuven, Belgium
    J Cell Biochem 89:663-73. 2003
    We investigated the capacity of 1,25-dihydroxyvitamin D(3) [1,25(OH)(2)D(3)] to protect human keratinocytes against the hazardous effects of ultraviolet B (UVB)-irradiation, recognized as the most important etiological factor in the ..
  47. ncbi ERK1/2 regulates epidermal chemokine expression and skin inflammation
    Saveria Pastore
    Laboratory of Immunology, Istituto Dermopatico dell Immacolata, Instituto di Ricovero e Cura a Carattere Scientifico, Roma, Italy
    J Immunol 174:5047-56. 2005
    ..TNF-alpha is a major inducer of proinflammatory mediators in keratinocytes. We have recently observed that epidermal growth factor receptor (EGFR) signaling affects TNF-alpha-driven ..
  48. ncbi Mechanical stress induces profound remodelling of keratin filaments and cell junctions in epidermolysis bullosa simplex keratinocytes
    David Russell
    Cancer Research UK Cell Structure Research Group, School of Life Sciences, MSI WTB complex, University of Dundee, Dundee, DD1 5EH, UK
    J Cell Sci 117:5233-43. 2004
    ..The results suggest that maintenance of desmosomes and hemidesmosomes may require some tension, normally mediated by keratin attachments...
  49. ncbi Areca (betel) nut extract activates mitogen-activated protein kinases and NF-kappaB in oral keratinocytes
    Shu Chun Lin
    Institute of Oral Biology, School of Dentistry, National Yang Ming University, Peitou, Taipei, Taiwan
    Int J Cancer 116:526-35. 2005
    ..We examined the activation of these signaling pathways in OECM-1 and SAS oral keratinocytes, treated with ripe areca nut extract (ANE). In both cells, a rapid increase in JNK1 activity at 0...
  50. pmc Activation of TLR2 by a small molecule produced by Staphylococcus epidermidis increases antimicrobial defense against bacterial skin infections
    Yuping Lai
    Division of Dermatology, Department of Medicine, University of California, San Diego and VA San Diego Healthcare System, San Diego, California 92161, USA
    J Invest Dermatol 130:2211-21. 2010
    ..the commensal microorganism Staphylococcus epidermidis may enhance production of antimicrobial peptides by keratinocytes and thus augment skin defense against infection...
  51. doi Cellular mechanisms regulating human melanogenesis
    H Y Park
    Department of Dermatology, Boston University School of Medicine, 609 Albany Street, Boston, MA 02118, USA
    Cell Mol Life Sci 66:1493-506. 2009
    ..Mature melanosomes are transferred to neighboring keratinocytes and are arranged in a supranuclear cap, protecting the DNA against incident ultraviolet light (UV) irradiation...
  52. pmc Linking the p53 tumour suppressor pathway to somatic cell reprogramming
    Teruhisa Kawamura
    Gene Expression Laboratory, Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, California 92037, USA
    Nature 460:1140-4. 2009
    ..These results provide insights into reprogramming mechanisms and suggest new routes to more efficient reprogramming while minimizing the use of oncogenes...
  53. ncbi Impaired PI3K/Akt activation-mediated NF-kappaB inactivation under elevated TNF-alpha is more vulnerable to apoptosis in vitiliginous keratinocytes
    Nan Hyung Kim
    Department of Dermatology, Dongguk University School of Medicine, Gyenggi do, South Korea
    J Invest Dermatol 127:2612-7. 2007
    ..B (PI3K/Akt) signaling pathway, was significantly higher in depigmented epidermis, implying that vitiliginous keratinocytes may be more susceptible to TNF-alpha-mediated apoptosis through impaired Akt and NF-kappaB activation...
  54. ncbi Absence of integrin alpha 6 leads to epidermolysis bullosa and neonatal death in mice
    E Georges-Labouesse
    Institut de Genetique et de Biologie Moleculaire et Cellulaire, CNRS INSERM ULP, Illkirch, C U de Strasbourg, France
    Nat Genet 13:370-3. 1996
    ..Our study provides evidence that some forms of epidermolysis bullosa may originate from defects of the alpha 6 gene...
  55. ncbi Activation of the IL-1beta-processing inflammasome is involved in contact hypersensitivity
    Hideki Watanabe
    Department of Dermatology, Geneva University Medical School, Geneva, Switzerland
    J Invest Dermatol 127:1956-63. 2007
    ..We show here that key components of the inflammasome are present in human keratinocytes and that CS like trinitro-chlorobenzene induce caspase-1/ASC dependent IL-1beta and IL-18 processing and ..
  56. doi IL-22 and IL-20 are key mediators of the epidermal alterations in psoriasis while IL-17 and IFN-gamma are not
    Kerstin Wolk
    Interdisciplinary Group of Molecular Immunopathology, Dermatology Medical Immunology, University Hospital Charite, Chariteplatz 1, 10117, Berlin, Germany
    J Mol Med (Berl) 87:523-36. 2009
    ..This cutaneous phenotype may be caused by the direct influence of IL-22 on keratinocytes, since this cytokine did not affect skin fibroblasts, endothelial cells, melanocytes, or adipocytes...
  57. ncbi The epidermal growth factor receptor system in skin repair and inflammation
    Saveria Pastore
    Laboratory of Tissue Engineering and Cutaneous Physiopathology, Istituto Dermopatico dell Immacolata, IRCCS, Via Monti di Creta 104, Rome, Italy
    J Invest Dermatol 128:1365-74. 2008
    ....
  58. pmc Selective antimicrobial action is provided by phenol-soluble modulins derived from Staphylococcus epidermidis, a normal resident of the skin
    Anna L Cogen
    Division of Dermatology, Department of Medicine, University of California San Diego, VA San Diego Health Care System, San Diego, California, USA
    J Invest Dermatol 130:192-200. 2010
    ..Thus, these data suggest that the production of PSMgamma and PSMdelta by S. epidermidis can benefit cutaneous immune defense by selectively inhibiting the survival of skin pathogens while maintaining the normal skin microbiome...
  59. pmc Epidermal stem cells are defined by global histone modifications that are altered by Myc-induced differentiation
    Michaela Frye
    Wellcome Trust Centre for Stem Cell Research, University of Cambridge, Cambridge, United Kingdom
    PLoS ONE 2:e763. 2007
    ..We conclude that Myc-induced chromatin modifications play a major role in Myc-induced exit from the stem cell compartment...
  60. pmc The Ink4/Arf locus is a barrier for iPS cell reprogramming
    Han Li
    Tumor Suppression Group, Spanish National Cancer Research Centre CNIO, 3 Melchor Fernandez Almagro Street, Madrid E 28029, Spain
    Nature 460:1136-9. 2009
    ..All together, we conclude that the silencing of Ink4/Arf locus is rate-limiting for reprogramming, and its transient inhibition may significantly improve the generation of iPS cells...
  61. pmc PKCη promotes a proliferation to differentiation switch in keratinocytes via upregulation of p27Kip1 mRNA through suppression of JNK/c-Jun signaling under stress conditions
    T Hara
    Department of Animal Resource Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113 8657, Japan
    Cell Death Dis 2:e157. 2011
    ..In 3D organotypic coculture with mouse keratinocytes and fibroblasts, the thickness of stratified cell layers was prolonged, and growth arrest and terminal ..
  62. pmc Staphylococcal peptidoglycan co-localizes with Nod2 and TLR2 and activates innate immune response via both receptors in primary murine keratinocytes
    Maria Anna Müller-Anstett
    Microbial Genetics, University of Tubingen, Tubingen, Germany
    PLoS ONE 5:e13153. 2010
    ..PGN(pol) was biotinylated (PGN-Bio) for fluorescence monitoring with specific antibodies. Keratinocytes from murine oral epithelium (MK) readily internalized PGN-Bio in an endocytosis-like process...
  63. ncbi Developmental transcription factor slug is required for effective re-epithelialization by adult keratinocytes
    Pierre Savagner
    Centre de Recherche en Cancerologie, CRLC Val d Aurelle Paul Lamarque, Montpellier Cedex 5, France
    J Cell Physiol 202:858-66. 2005
    During re-epithelialization of cutaneous wounds, keratinocytes recapitulate several aspects of the embryonic process of epithelial-mesenchymal transition (EMT), including migratory activity and reduced intercellular adhesion...
  64. pmc Generation of keratinocytes from normal and recessive dystrophic epidermolysis bullosa-induced pluripotent stem cells
    Munenari Itoh
    Departments of Dermatology, Columbia University, New York, NY 10032, USA
    Proc Natl Acad Sci U S A 108:8797-802. 2011
    ..requires successful differentiation of iPSCs into cellular components of the skin, including epidermal keratinocytes. Here, we succeeded in generating iPSCs not only from normal human fibroblasts but also from fibroblasts ..
  65. pmc Cooperation between the transcription factors p63 and IRF6 is essential to prevent cleft palate in mice
    Helen A Thomason
    Faculty of Medical and Human Sciences, Manchester Academic Health Sciences Centre, University of Manchester, Manchester, United Kingdom
    J Clin Invest 120:1561-9. 2010
    ..Here, we used both mouse models and human primary keratinocytes from patients with cleft palate to demonstrate that IRF6 and p63 interact epistatically during development of ..
  66. pmc Transient receptor potential vanilloid 4 (TRPV4)-dependent calcium influx and ATP release in mouse oesophageal keratinocytes
    Hiroshi Mihara
    Division of Cell Signaling, Okazaki Institute for Integrative Bioscience, National Institutes of Natural Sciences, Okazaki, Aichi 444 8787, Japan
    J Physiol 589:3471-82. 2011
    ..TRPV4 is expressed in many tissues, including renal epithelium, skin keratinocytes and urinary bladder epithelium, but its expression and function in the oesophagus is poorly understood...
  67. ncbi Immunopathogenesis of psoriasis
    Robert Sabat
    Interdisciplinary Group of Molecular Immunopathology, Dermatology Medical Immunology, University Hospital Charite, Berlin, Germany
    Exp Dermatol 16:779-98. 2007
    ..Our model helps to explain the varied effectiveness of the currently tested immune modulating therapies and may enable the prediction of the success of future therapies...
  68. ncbi The inflammasome mediates UVB-induced activation and secretion of interleukin-1beta by keratinocytes
    Laurence Feldmeyer
    Institute of Cell Biology, Department of Biology, ETH Zurich, Zurich, Switzerland
    Curr Biol 17:1140-5. 2007
    It has long been known that human keratinocytes are a potent source of the proinflammatory cytokines proIL-1alpha and -1beta[1], which are activated and released in response to UV irradiation [2]...
  69. doi Flagellin is the principal inducer of the antimicrobial peptide S100A7c (psoriasin) in human epidermal keratinocytes exposed to Escherichia coli
    Arby Abtin
    Department of Dermatology, Medical University of Vienna, Waehringer Guertal 18 20, 1090 Vienna, Austria
    FASEB J 22:2168-76. 2008
    Epidermal keratinocytes (KCs) express antimicrobial peptides as a part of the innate immune response...
  70. pmc Galectin-3 protects keratinocytes from UVB-induced apoptosis by enhancing AKT activation and suppressing ERK activation
    Jun Saegusa
    Department of Dermatology, University of California, Davis, School of Medicine, Sacramento, California 95816, USA
    J Invest Dermatol 128:2403-11. 2008
    b>Keratinocytes undergo apoptosis in a variety of physiological and pathological conditions. Galectin-3 is a member of a family of beta-galactoside-binding animal lectins expressed abundantly in keratinocytes and other epithelial cells...
  71. doi Efficient and rapid generation of induced pluripotent stem cells from human keratinocytes
    Trond Aasen
    Center of Regenerative Medicine in Barcelona, Dr Aiguader 88, 08003 Barcelona, Spain
    Nat Biotechnol 26:1276-84. 2008
    ..Here we show that reprogramming of juvenile human primary keratinocytes by retroviral transduction with OCT4, SOX2, KLF4 and c-MYC is at least 100-fold more efficient and twofold ..
  72. doi Anti-apoptotic role of S100A8 in X-ray irradiated keratinocytes
    Young Sook Lee
    Department of Dermatology and Research Institute of Medical Sciences, School of Medicine, Chungnam National University, 640 Daesa dong, Daejeon, Republic of Korea
    J Dermatol Sci 51:11-8. 2008
    ..We previously established an animal model for radiodermatitis, and identified many of radiation-induced genes by cDNA microarray. Of the candidates, we chose S100A8 gene for a further study...
  73. pmc Human papillomavirus E1 helicase interacts with the WD repeat protein p80 to promote maintenance of the viral genome in keratinocytes
    Alexandra Côté-Martin
    Laboratory of Molecular Virology, Institut de Recherches Cliniques de Montreal, 110 Pine Avenue West, Montreal, Quebec, Canada
    J Virol 82:1271-83. 2008
    ..HPV31 genomes encoding two of the mutant E1 proteins were not maintained as episomes in immortalized primary keratinocytes, whereas one encoding the third mutant protein was maintained at a very low copy number...
  74. pmc Th17 cytokines stimulate CCL20 expression in keratinocytes in vitro and in vivo: implications for psoriasis pathogenesis
    Erin G Harper
    Department of Dermatology, Oregon Health and Science University, Portland, Oregon 97239, USA
    J Invest Dermatol 129:2175-83. 2009
    ....
  75. ncbi Fos-related antigen (Fra-1), junB, and junD activate human involucrin promoter transcription by binding to proximal and distal AP1 sites to mediate phorbol ester effects on promoter activity
    J F Welter
    Department of Physiology and Biophysics, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106 4970, USA
    J Biol Chem 270:12614-22. 1995
    ..Using reporter gene constructs in human keratinocytes, we show that the most distal (AP1-5) and most proximal (AP1-1) AP1 sites are essential for high level ..
  76. ncbi An extended epidermal response heals cutaneous wounds in the absence of a hair follicle stem cell contribution
    Abigail K Langton
    Faculty of Life Sciences, University of Manchester, Manchester, UK
    J Invest Dermatol 128:1311-8. 2008
    ..In analyzing stem cell behavior in embryonic skin we found that clonogenic keratinocytes are relatively frequent in the ectoderm prior to hair follicle formation...
  77. doi Innate immune responses to Mycobacterium ulcerans via toll-like receptors and dectin-1 in human keratinocytes
    Hye Mi Lee
    Department of Microbiology, Chungnam National University, Daejeon, South Korea
    Cell Microbiol 11:678-92. 2009
    ..We hypothesized that epidermal keratinocytes might not be a simple barrier, but play a role during MU infection through pattern-recognition receptors ..
  78. pmc SIRT1 promotes differentiation of normal human keratinocytes
    Gil Blander
    Genstruct Inc, One Alewife Center, Cambridge, Massachusetts 2140, USA
    J Invest Dermatol 129:41-9. 2009
    ..of SIRT1 in normal human keratinocyte physiology by a transcriptional microarray analysis of primary keratinocytes either overexpressing or underexpressing SIRT1...
  79. ncbi A role for skin gammadelta T cells in wound repair
    Julie Jameson
    Department of Immunology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Science 296:747-9. 2002
    ..dendritic epidermal T cells (DETCs) found in murine skin recognize antigen expressed by damaged or stressed keratinocytes. Activated DETCs produce keratinocyte growth factors (KGFs) and chemokines, raising the possibility that DETCs ..
  80. doi UV irradiation induces Snail expression by AP-1 dependent mechanism in human skin keratinocytes
    Yong Li
    Department of Dermatology, University of Michigan, Ann Arbor, MI 48109 5609, USA
    J Dermatol Sci 60:105-13. 2010
    ..Ultraviolet (UV) irradiation from the sun is the major cause of keratinocyte skin cancer. Transcription factor Snail plays an important role in epithelial-to-mesenchymal transition and epithelial tumor formation...
  81. pmc A FoxO-Smad synexpression group in human keratinocytes
    Roger R Gomis
    Cancer Biology and Genetics Program, Howard Hughes Medical Institute, and Bioinformatics Core Facility, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 103:12747-52. 2006
    ..To delineate the organization of the TGF-beta response in human keratinocytes, we defined the set of genes whose activation by TGF-beta requires both FoxO and Smad functions...
  82. pmc Specific TRPC6 channel activation, a novel approach to stimulate keratinocyte differentiation
    Margarethe Müller
    Institute of Pharmacology, Biocenter Niederursel, Johann Wolfgang Goethe University, Max von Laue Strasse 9, 60439 Frankfurt, Germany
    J Biol Chem 283:33942-54. 2008
    ..in our skin undergoes constant regulation, whereby the balance between differentiation and proliferation of keratinocytes plays a major role...
  83. ncbi Regulation of vascular endothelial growth factor expression in cultured keratinocytes. Implications for normal and impaired wound healing
    S Frank
    Max Planck Institut fur Biochemie, Martinsried, Germany
    J Biol Chem 270:12607-13. 1995
    ..of VEGF induction during this process, we analyzed the regulation of VEGF expression in cultured human keratinocytes. We found a large induction of VEGF expression upon treatment of quiescent cells with serum, epidermal growth ..
  84. pmc HPV E7 contributes to the telomerase activity of immortalized and tumorigenic cells and augments E6-induced hTERT promoter function
    Xuefeng Liu
    Department of Pathology and Oncology, Georgetown University Medical School, Washington, DC 20057, USA
    Virology 375:611-23. 2008
    The E6 and E7 proteins of high-risk HPVs are both required for the immortalization of primary human keratinocytes and the maintenance of the malignant phenotype of HPV-positive cancer cell lines...
  85. ncbi Increased serine protease activity and cathelicidin promotes skin inflammation in rosacea
    Kenshi Yamasaki
    Division of Dermatology, University of California, San Diego, and VA San Diego Health Care System, 3350 La Jolla Village Drive, San Diego, California 92161, USA
    Nat Med 13:975-80. 2007
    ..These findings confirm the role of cathelicidin in skin inflammatory responses and suggest an explanation for the pathogenesis of rosacea by demonstrating that an exacerbated innate immune response can reproduce elements of this disease...
  86. pmc Angiopoietin-like 4 interacts with integrins beta1 and beta5 to modulate keratinocyte migration
    Yan Yih Goh
    School of Biological Sciences, Nanyang Technological University, Singapore 637551
    Am J Pathol 177:2791-803. 2010
    ..Upon skin injury, keratinocytes use various signaling molecules to promote reepithelialization for efficient wound closure...
  87. ncbi Current insights into the formation and breakdown of hemidesmosomes
    Sandy H M Litjens
    Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    Trends Cell Biol 16:376-83. 2006
    ..is of crucial importance in a variety of biological processes, such as differentiation and migration of keratinocytes during wound healing and carcinoma invasion, in which cells become detached from the substrate and acquire a ..
  88. pmc The E6 oncoproteins from human betapapillomaviruses differentially activate telomerase through an E6AP-dependent mechanism and prolong the lifespan of primary keratinocytes
    Kristin M Bedard
    Division of Human Biology, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, C1 015, Box 19024, Seattle, WA 98109 1024, USA
    J Virol 82:3894-902. 2008
    ..We show that human foreskin keratinocytes (HFKs) expressing several betapapillomavirus E6 (beta-E6) proteins display life span extension, but not to the ..
  89. ncbi Differentiation of mouse keratinocytes is accompanied by PKC-dependent changes in AP-1 proteins
    S E Rutberg
    Laboratory of Cellular Carcinogenesis and Tumor Promotion, National Cancer Institute, Bethesda, Maryland 20892, USA
    Oncogene 13:167-76. 1996
    The conversion of cultured basal keratinocytes to the spinous and granular cell phenotypes seen in the skin can be stimulated by raising the levels of extracellular calcium...
  90. doi Protein kinase C delta and eta differently regulate the expression of loricrin and Jun family proteins in human keratinocytes
    Nagisa Kamioka
    Institute of Molecular Oncology, Showa University, 1 5 8 Hatanodai, Shinagawa ku, Tokyo 142 8555, Japan
    Biochem Biophys Res Commun 394:106-11. 2010
    ..However, loricrin expression was inversely controlled by PKCdelta and PKCeta in cultured keratinocytes and 3D skin culture; i.e. loricrin expression was decreased by PKCdelta and increased by PKCeta...
  91. doi Isolation and cultivation of human keratinocytes from skin or plucked hair for the generation of induced pluripotent stem cells
    Trond Aasen
    Center of Regenerative Medicine in Barcelona, Barcelona, Spain
    Nat Protoc 5:371-82. 2010
    ..Reprogramming of keratinocytes is both faster and more efficient compared with fibroblasts, although more care is required when isolating, ..
  92. doi Use of IL-18 production in a human keratinocyte cell line to discriminate contact sensitizers from irritants and low molecular weight respiratory allergens
    Emanuela Corsini
    Department of Pharmacological Sciences, Faculty of Pharmacy, Laboratory of Toxicology, University of Milan, Milan, Italy
    Toxicol In Vitro 23:789-96. 2009
    ..Similar results were also obtained using primary human keratinocytes. Results were reproducible, and the method could be transferred to another laboratory, suggesting the ..
  93. pmc T cell-mediated Fas-induced keratinocyte apoptosis plays a key pathogenetic role in eczematous dermatitis
    A Trautmann
    Swiss Institute of Allergy and Asthma Research SIAF, Davos, Switzerland
    J Clin Invest 106:25-35. 2000
    ..Responses of normal human skin and cultured skin equivalents to activated T cells demonstrated that KC apoptosis caused by skin-infiltrating T cells is a key event in the pathogenesis of eczematous dermatitis...
  94. pmc Wnt5a exhibits layer-specific expression in adult skin, is upregulated in psoriasis, and synergizes with type 1 interferon
    Malgorzata Romanowska
    Division of Experimental Medicine, University of Dundee, Dundee, Scotland, UK
    PLoS ONE 4:e5354. 2009
    ..The expression and distribution of Wnt5a and its receptors frizzled (fzd) 3 and fzd 5 in adult human skin have not been comprehensively studied to date...
  95. doi Somatostatin regulates tight junction function and composition in human keratinocytes
    Matthias Vockel
    Department of Dermatology and Venerology, University Hospital Hamburg Eppendorf, Hamburg, Germany
    Exp Dermatol 19:888-94. 2010
    ..SST is expressed in human skin and is present in a subset of Merkel cells and dendritic cells as well as in keratinocytes. We focused further on the somatostatin receptor subtype 3 (SSTR3) and its interacting protein MUPP1, as both ..
  96. pmc Prevention of UVB radiation-induced epidermal damage by expression of heat shock protein 70
    Minoru Matsuda
    Graduate School of Medical and Pharmaceutical Sciences, Kumamoto University, Kumamoto 862 0973, Japan
    J Biol Chem 285:5848-58. 2010
    ..Here we tested this idea by using transgenic mice expressing HSP70 and cultured keratinocytes. Irradiation of wild-type mice with UVB caused epidermal damage such as induction of apoptosis, which was ..
  97. doi Possible roles of IL-27 in the pathogenesis of psoriasis
    Sayaka Shibata
    Faculty of Medicine, Department of Dermatology, University of Tokyo, Tokyo, Japan
    J Invest Dermatol 130:1034-9. 2010
    ..production and tyrosine phosphorylation of signal transducer and activator of transcription 1 in normal human keratinocytes, while it suppressed the tumor necrosis factor-alpha-induced production of IL-1alpha and CCL20...
  98. doi Injury is a major inducer of epidermal innate immune responses during wound healing
    K Markus Roupé
    Division of Infection Medicine, Lund University, Lund, Sweden
    J Invest Dermatol 130:1167-77. 2010
    ..Conceptually, these data show that the growth factor response elicited by injury is important for the recruitment of neutrophils in skin wounds...
  99. pmc Activators of PPARs and LXR decrease the adverse effects of exogenous glucocorticoids on the epidermis
    Marianne Demerjian
    Department of Dermatology, UCSF and VA Medical Center, San Francisco, CA 94121, USA
    Exp Dermatol 18:643-9. 2009
    ..Together, these studies demonstrate that PPAR and LXR activators can prevent several of the adverse effects of topical GC on the epidermis...
  100. pmc Fibroblast growth factor receptors 1 and 2 in keratinocytes control the epidermal barrier and cutaneous homeostasis
    Jingxuan Yang
    Department of Biology, Institute of Cell Biology, Eidgenossische Technische Hochschule Zurich, 8093 Zurich, Switzerland
    J Cell Biol 188:935-52. 2010
    ..Loss of the IIIb splice variants of FGFR1 and FGFR2 in keratinocytes caused progressive loss of skin appendages, cutaneous inflammation, keratinocyte hyperproliferation, and ..
  101. doi Oncogenic H-Ras and PI3K signaling can inhibit E-cadherin-dependent apoptosis and promote cell survival after photodynamic therapy in mouse keratinocytes
    Jesus Espada
    Instituto de Investigaciones Biomedicas Alberto Sols, CSIC Departamento de Bioquímica, Facultad de Medicina, Universidad Autonoma de Madrid, Madrid, Spain
    J Cell Physiol 219:84-93. 2009
    ..Here we report that oncogenic activation of H-Ras in murine keratinocytes can prevent cell death induced by immunological disruption of E-cadherin adhesion...

Research Grants102 found, 100 shown here

  1. Role of CRH in skin physiology and pathology
    Andrzej Slominski; Fiscal Year: 2004
    ..To define local mechanisms regulating the expression of CRH in normal melanocytes and keratinocytes; 2...
  2. CHARACTERIZATION OF NEUROPILIN, A NOVEL VEGF RECEPTOR
    Michael Klagsbrun; Fiscal Year: 2007
    ..We have found so far that phorbol ester, EGF and HB-EGF induce NRPl promoter activity in EC, tumor cells and keratinocytes. Future studies include: i) using NRP promoter-reporter constructs to screen inducers and inhibitors of NRP ..
  3. Somatic Stem Cells in Engineered Human Skin
    Steven Boyce; Fiscal Year: 2009
    ..skin substitutes (CSS) consisting of a collagen-based sponge populated with cultured human fibroblasts and keratinocytes. This model of engineered human skin develops a keratinized epithelium, basement membrane, and has a dermal ..
  4. Role of OCT4 in reprogramming human skin keratinocytes
    Jackie R Bickenbach; Fiscal Year: 2010
    ..Later in 2006, we published that mouse skin keratinocytes could be reprogrammed using an alternative approach, transient transfection of the single embryonic gene Oct4...
  5. Plexin signalling in melanocyte biology and melanoma progression
    Glynis A Scott; Fiscal Year: 2010
    ..photoprotection of the skin from ultraviolet radiation (UVR) through the production and transfer of melanin to keratinocytes [1]...
  6. The Role of bacterial toxins in human skin disease.
    DONALD YM contact LEUNG; Fiscal Year: 2010
    ..data show that staphylococcal superantigens (staphylococcal enterotoxin B, SEB) also exert effects directly on keratinocytes and contribute to the skin inflammation by inducing proinflammatory and Th2 cell skin homing chemokines...
  7. Somatic Stem Cells in Engineered Human Skin
    Steven Boyce; Fiscal Year: 2009
    ..skin substitutes (CSS) consisting of a collagen-based sponge populated with cultured human fibroblasts and keratinocytes. This model of engineered human skin develops a keratinized epithelium, basement membrane, and has a dermal ..
  8. THE IDENTITY OF THE LATENT NEOPLASTIC EPIDERMAL CELL
    Rebecca Morris; Fiscal Year: 1993
    ..The overall objective is to test this. In Aim 1, freshly isolated epidermal keratinocytes (including those from the hair follicles) from adult mice are sorted by flow cytometry following staining with ..
  9. Trim32 Regulation of Piasy in Skin Homeostasis
    Molly Kulesz Martin; Fiscal Year: 2009
    There is a vicious circle in psoriasis that disrupts epidermal homeostasis through alterations in keratinocytes (hyperproliferation, parakeratosis) and immunocytes (infiltration and activation)...
  10. Trim32 Regulation of Piasy in Skin Homeostasis
    Molly F Kulesz Martin; Fiscal Year: 2010
    There is a vicious circle in psoriasis that disrupts epidermal homeostasis through alterations in keratinocytes (hyperproliferation, parakeratosis) and immunocytes (infiltration and activation)...
  11. Calcium-sensing Receptor and Keratinocyte Differentiation
    Chia Ling Tu; Fiscal Year: 2010
    Epidermis consists of multiple layers of keratinocytes, which differentiate and produce a permeability barrier that provides protection against environmental insults...
  12. Entry of Oncogenic HPVs into Human Keratinocytes
    MICHELLE OZBUN; Fiscal Year: 2009
    ..we show evidence that oncogenic HPV types HPV16 and HPV31 interact differently with normal host cells, human keratinocytes (HKs)...
  13. Entry of Oncogenic HPVs into Human Keratinocytes
    Michelle A Ozbun; Fiscal Year: 2010
    ..we show evidence that oncogenic HPV types HPV16 and HPV31 interact differently with normal host cells, human keratinocytes (HKs)...
  14. CALCIUM, S100 PROTEINS AND KERATINOCYTE DIFFERENTIATION
    RICHARD ECKERT; Fiscal Year: 2004
    ..The target proteins include regulators of cell proliferation and differentiation. Although keratinocytes express eight S100 proteins, little information is available regarding S100 protein function in keratinocytes...
  15. P38 MAP KINASE SIGNALING IN SKIN
    Tatiana Efimova; Fiscal Year: 2010
    ..data, obtained since the previous review of this application, show that oncogenic ras-transformed p384-null keratinocytes formed smaller skin tumors in orthotopic skin grafts to p384 intact hosts, compared with transformed wild-type ..
  16. Keratin proteins and non-melanoma skin tumors
    PIERRE COULOMBE; Fiscal Year: 2007
    ..Examples of functions that are so far unique to K17 in skin epithelia are the protection of keratinocytes against TNFa-mediated apoptosis, which is manifested during the anagen (growth) phase in cycling hair ..
  17. Regulation of Skin Wound Epithelialization by Galectin-3
    Fu Tong Liu; Fiscal Year: 2007
    ..Galectin-3 is the most extensively studied member and is expressed by a variety of cells, including keratinocytes and macrophages...
  18. Keratinocyte responses to vascular endothelial growth factor in carcinogenesis
    Traci A Wilgus; Fiscal Year: 2010
    ..Recently, our laboratory has discovered that one of these receptors, VEGFR-1, is expressed by epidermal keratinocytes. We have also demonstrated a functional role for VEGFR-1 in keratinocyte proliferation in vitro and in vivo ..
  19. Keratinocyte responses to vascular endothelial growth factor in carcinogenesis
    TRACI WILGUS; Fiscal Year: 2009
    ..Recently, our laboratory has discovered that one of these receptors, VEGFR-1, is expressed by epidermal keratinocytes. We have also demonstrated a functional role for VEGFR-1 in keratinocyte proliferation in vitro and in vivo ..
  20. Keratinocyte responses to vascular endothelial growth factor in carcinogenesis
    TRACI WILGUS; Fiscal Year: 2007
    ..Recently, our laboratory has discovered that one of these receptors, VEGFR-1, is expressed by epidermal keratinocytes. We have also demonstrated a functional role for VEGFR-1 in keratinocyte proliferation in vitro and in vivo ..
  21. Role of OCT4 in reprogramming human skin keratinocytes
    Jackie R Bickenbach; Fiscal Year: 2011
    ..Later in 2006, we published that mouse skin keratinocytes could be reprogrammed using an alternative approach, transient transfection of the single embryonic gene Oct4...
  22. Role of Wnt signaling in Cervical Cancer Pathogenesis
    Aykut Uren; Fiscal Year: 2010
    ..Our experimental model of cervical carcinoma uses HPV-immortalized primary human keratinocytes. In an in vitro experimental model, malignant transformation of HPV-immortalized keratinocytes can be achieved ..
  23. Role of Wnt signaling in Cervical Cancer Pathogenesis
    Aykut Uren; Fiscal Year: 2009
    ..Our experimental model of cervical carcinoma uses HPV-immortalized primary human keratinocytes. In an in vitro experimental model, malignant transformation of HPV-immortalized keratinocytes can be achieved ..
  24. ADHESION MOLECULES AND EPIDERMAL STRATIFICATION
    ROBERT LAVKER; Fiscal Year: 2001
    The protective function of the epidermis depends critically on the normal differentiation of the keratinocytes. Cell to cell adhesion is essential in this process...
  25. MECHANISMS OF RE-EPITHELIALIZATION OF SKIN WOUNDS
    PIERRE COULOMBE; Fiscal Year: 1999
    ..It has been found that suprabasal, differentiating keratinocytes are the major source of epithelial cells that become activated and then migrate to cover the wound surface...
  26. CELL CELL COMMUNICATION DURING MELANOMA DEVELOPMENT
    Meenhard Herlyn; Fiscal Year: 2004
    DESCRIPTION: (Applicant's abstract) In the human epidermis, melanocytes and keratinocytes maintain a lifelong homeostatic balance, which is only disturbed during transformation...
  27. MECHANISMS OF DENDRITE FORMATION & MELANOSOME TRANSFER
    Glynis Scott; Fiscal Year: 2003
    The effective transfer of melanosomes, specialized organelles for melanin production, from melanocytes to keratinocytes, is an absolute requirement for photoprotection in the skin...
  28. ROLE OF IL-23 IN PSORIASIS PATHOGENESIS
    Andrew Blauvelt; Fiscal Year: 2007
    ..In humans, recent reports indicate that IL-23 produced by keratinocytes and activated dendritic cells, TGF¿1 produced by keratinocytes, and IL-17A produced by Th17 cells are ..
  29. Mechanisms of cancer initiation by TRIM32
    Molly F Kulesz Martin; Fiscal Year: 2010
    ..Piasy is a pro- apoptotic factor, inducing apoptosis in CML cells, human fibroblasts and mouse keratinocytes. We show that Trim32 protects keratinocytes from apoptosis induced by UVB and synergized by TNFa...
  30. Mechanisms of cancer initiation by TRIM32
    Molly Kulesz Martin; Fiscal Year: 2009
    ..Piasy is a pro- apoptotic factor, inducing apoptosis in CML cells, human fibroblasts and mouse keratinocytes. We show that Trim32 protects keratinocytes from apoptosis induced by UVB and synergized by TNFa...
  31. MECHANISMS OF INDUCTION OF SKIN CANCERS BY UV LIGHT
    HONNAVARA ANANTHASWAMY; Fiscal Year: 2004
    ..extremely resistant to UV carcinogenesis and phototoxicity, and their skin contains a high number of apoptotic keratinocytes compared to CD1+/+ mice...
  32. Mechanisms of cancer initiation by TRIM32
    Molly Kulesz Martin; Fiscal Year: 2009
    ..Piasy is a pro- apoptotic factor, inducing apoptosis in CML cells, human fibroblasts and mouse keratinocytes. We show that Trim32 protects keratinocytes from apoptosis induced by UVB and synergized by TNFa...
  33. Mechanisms of cancer initiation by TRIM32
    Molly F Kulesz Martin; Fiscal Year: 2010
    ..Piasy is a pro- apoptotic factor, inducing apoptosis in CML cells, human fibroblasts and mouse keratinocytes. We show that Trim32 protects keratinocytes from apoptosis induced by UVB and synergized by TNFa...
  34. IkB Kinase alpha (IKKalpha): Role in Skin Carcinogenesis
    Yinling Hu; Fiscal Year: 2007
    ..Our preliminary results show that the immortalized IKKalpha-/- keratinocytes are able to form cell colonies in soft agar and grow into poorly differentiated spindle cell carcinomas in ..
  35. Amphiregulin in Psoriatic Epidermal Hyperplasia
    JAMES TILFORD ELDER; Fiscal Year: 2010
    ..However, the mechanism by which immunologic activation triggers epidermal hyperplasia remains unknown. Keratinocytes overexpress multiple ErbB1 ligands in psoriasis, including amphiregulin (AR), TGF-a, and HB- EGF...