granulosa cells

Summary

Summary: Supporting cells for the developing female gamete in the OVARY. They are derived from the coelomic epithelial cells of the gonadal ridge. Granulosa cells form a single layer around the OOCYTE in the primordial ovarian follicle and advance to form a multilayered cumulus oophorus surrounding the OVUM in the Graafian follicle. The major functions of granulosa cells include the production of steroids and LH receptors (RECEPTORS, LH).

Top Publications

  1. pmc Lipopolysaccharide initiates inflammation in bovine granulosa cells via the TLR4 pathway and perturbs oocyte meiotic progression in vitro
    John J Bromfield
    Institute of Life Science, School of Medicine, Swansea University, Singleton Park, Swansea SA2 8PP, United Kingdom
    Endocrinology 152:5029-40. 2011
  2. ncbi Follicular growth and atresia in mammalian ovaries: regulation by survival and death of granulosa cells
    Fuko Matsuda
    Laboratory of Animal Production Science, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464 8601, Japan
    J Reprod Dev 58:44-50. 2012
  3. ncbi Follicle-stimulating hormone regulation of microRNA expression on progesterone production in cultured rat granulosa cells
    Nan Yao
    Graduate School of Peking Union Medical College, Beijing, China
    Endocrine 38:158-66. 2010
  4. pmc Marine compounds selectively induce apoptosis in female reproductive cancer cells but not in primary-derived human reproductive granulosa cells
    Vicki Edwards
    Department of Medical Biotechnology, School of Medicine, Flinders University, GPO Box 2100, Adelaide, S A 5001, Australia
    Mar Drugs 10:64-83. 2012
  5. ncbi The murine winged-helix transcription factor Foxl2 is required for granulosa cell differentiation and ovary maintenance
    Dirk Schmidt
    Developmental Biology Programme, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Development 131:933-42. 2004
  6. pmc Hormonal regulation of MicroRNA expression in periovulatory mouse mural granulosa cells
    Stephanie D Fiedler
    Department of Molecular and Integrative Physiology, University of Kansas Medical Center, Kansas City, Kansas 66160, USA
    Biol Reprod 79:1030-7. 2008
  7. pmc MicroRNA 21 blocks apoptosis in mouse periovulatory granulosa cells
    Martha Z Carletti
    Department of Molecular and Integrative Physiology, University of Kansas Medical Center, Kansas City, Kansas, USA
    Biol Reprod 83:286-95. 2010
  8. ncbi Intercellular communication in the mammalian ovary: oocytes carry the conversation
    Martin M Matzuk
    Department of Pathology, Baylor College of Medicine, 1 Baylor Plaza, Houston, TX 77030, USA
    Science 296:2178-80. 2002
  9. ncbi Saturated FFAs, palmitic acid and stearic acid, induce apoptosis in human granulosa cells
    Y M Mu
    Third Department of Internal Medicine, Faculty of Medicine, Kyushu University, Higashi-ku, Fukuoka 812-8582, Japan
    Endocrinology 142:3590-7. 2001
  10. ncbi Formation of the ovarian follicular antrum and follicular fluid
    Raymond J Rodgers
    Research Centre for Reproductive Health, Discipline of Obstetrics and Gynaecology, School of Paediatrics and Reproductive Health, Robinson Institute, The University of Adelaide, Adelaide, South Australia, Australia
    Biol Reprod 82:1021-9. 2010

Detail Information

Publications394 found, 100 shown here

  1. pmc Lipopolysaccharide initiates inflammation in bovine granulosa cells via the TLR4 pathway and perturbs oocyte meiotic progression in vitro
    John J Bromfield
    Institute of Life Science, School of Medicine, Swansea University, Singleton Park, Swansea SA2 8PP, United Kingdom
    Endocrinology 152:5029-40. 2011
    ..that lipopolysaccharide (LPS) from Gram-negative bacteria stimulates an inflammatory response by ovarian granulosa cells that is mediated by Toll-like receptor (TLR) 4...
  2. ncbi Follicular growth and atresia in mammalian ovaries: regulation by survival and death of granulosa cells
    Fuko Matsuda
    Laboratory of Animal Production Science, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464 8601, Japan
    J Reprod Dev 58:44-50. 2012
    ..b>Granulosa cells in particular have been demonstrated to play a major role in deciding the fate of follicles, serving ..
  3. ncbi Follicle-stimulating hormone regulation of microRNA expression on progesterone production in cultured rat granulosa cells
    Nan Yao
    Graduate School of Peking Union Medical College, Beijing, China
    Endocrine 38:158-66. 2010
    ..Here we show that expression of 31 miRNAs is altered during FSH-mediated progesterone secretion of cultured granulosa cells. Specifically, 12 h after FSH treatment, miRNAs mir-29a and mir-30d were significantly down-regulated...
  4. pmc Marine compounds selectively induce apoptosis in female reproductive cancer cells but not in primary-derived human reproductive granulosa cells
    Vicki Edwards
    Department of Medical Biotechnology, School of Medicine, Flinders University, GPO Box 2100, Adelaide, S A 5001, Australia
    Mar Drugs 10:64-83. 2012
    ..and 6-bromoisatin from Dicathais orbita were tested against physiologically normal primary human granulosa cells (HGC) and reproductive cancer cell lines...
  5. ncbi The murine winged-helix transcription factor Foxl2 is required for granulosa cell differentiation and ovary maintenance
    Dirk Schmidt
    Developmental Biology Programme, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Development 131:933-42. 2004
    ..In Foxl2(lacZ) homozygous mutant ovaries granulosa cells do not complete the squamous to cuboidal transition leading to the absence of secondary follicles and oocyte ..
  6. pmc Hormonal regulation of MicroRNA expression in periovulatory mouse mural granulosa cells
    Stephanie D Fiedler
    Department of Molecular and Integrative Physiology, University of Kansas Medical Center, Kansas City, Kansas 66160, USA
    Biol Reprod 79:1030-7. 2008
    ..The extent and hormonal regulation of miRNA expression by ovarian granulosa cells and their role in ovulation and luteinization is unknown...
  7. pmc MicroRNA 21 blocks apoptosis in mouse periovulatory granulosa cells
    Martha Z Carletti
    Department of Molecular and Integrative Physiology, University of Kansas Medical Center, Kansas City, Kansas, USA
    Biol Reprod 83:286-95. 2010
    ..Transition of proliferative ovarian granulosa cells to terminally differentiated luteal cells in response to the ovulatory surge of luteinizing hormone (LH) ..
  8. ncbi Intercellular communication in the mammalian ovary: oocytes carry the conversation
    Martin M Matzuk
    Department of Pathology, Baylor College of Medicine, 1 Baylor Plaza, Houston, TX 77030, USA
    Science 296:2178-80. 2002
    ..The challenge for the future is to identify the factors that participate in this communication and their mechanisms of action...
  9. ncbi Saturated FFAs, palmitic acid and stearic acid, induce apoptosis in human granulosa cells
    Y M Mu
    Third Department of Internal Medicine, Faculty of Medicine, Kyushu University, Higashi-ku, Fukuoka 812-8582, Japan
    Endocrinology 142:3590-7. 2001
    ..A Western blot analysis showed the apoptosis of the granulosa cells induced by palmitic acid to be accompanied by the down-regulation of an apoptosis inhibitor, Bcl-2, and the ..
  10. ncbi Formation of the ovarian follicular antrum and follicular fluid
    Raymond J Rodgers
    Research Centre for Reproductive Health, Discipline of Obstetrics and Gynaecology, School of Paediatrics and Reproductive Health, Robinson Institute, The University of Adelaide, Adelaide, South Australia, Australia
    Biol Reprod 82:1021-9. 2010
    ..The central hypothesis on follicular fluid formation suggests that production by granulosa cells of hyaluronan and the chondroitin sulfate proteoglycan versican generates an osmotic gradient...
  11. ncbi Evidence that Sry is expressed in pre-Sertoli cells and Sertoli and granulosa cells have a common precursor
    K H Albrecht
    The Jackson Laboratory, 600 Main Street, Bar Harbor, Maine 04609, USA
    Dev Biol 240:92-107. 2001
    ..and is hypothesized to do so by directing supporting cell precursors to develop as Sertoli cells and not as granulosa cells. To directly test this hypothesis, transgenic mice expressing EGFP under the control of the Sry promoter were ..
  12. ncbi A molecular mechanism underlying ovarian dysfunction of polycystic ovary syndrome: hyperandrogenism induces epigenetic alterations in the granulosa cells
    Fan Qu
    Department of Reproductive Endocrinology, Women s Hospital, School of Medicine, Zhejiang University, 1 Xueshi Road, Hangzhou, Zhejiang 310006, China
    J Mol Med (Berl) 90:911-23. 2012
    ..receptor gamma 1 (PPARG1), nuclear corepressor 1 (NCOR1), and histone deacetylase 3 (HDAC3) genes in granulosa cells (GCs) of polycystic ovary syndrome (PCOS) women and whether these alterations are involved in the ovarian ..
  13. ncbi Distinct regulation by steroids of messenger RNAs for FSHR and CYP19A1 in bovine granulosa cells
    Wenxiang Luo
    Endocrinology Reproductive Physiology Program and Department of Dairy Science, University of Wisconsin, Madison, Wisconsin 53706, USA
    Biol Reprod 75:217-25. 2006
    Steroidal regulation of gene expression in follicular cells is not completely defined. Granulosa cells from 5 mm bovine follicles were cultured and treated and steady-state mRNA levels determined for FSHR (follicle-stimulating hormone ..
  14. ncbi Expression of anti-Müllerian hormone during normal and pathological gonadal development: association with differentiation of Sertoli and granulosa cells
    E Rajpert-De Meyts
    Department of Growth and Reproduction, Copenhagen University Hospital Rigshospitalet, Denmark
    J Clin Endocrinol Metab 84:3836-44. 1999
    ..AMH expression was found only in Sertoli and granulosa cells. A 6-week-old fetal testis at the indifferent gonad stage did not yet express AMH...
  15. ncbi Transactivation of microRNA-383 by steroidogenic factor-1 promotes estradiol release from mouse ovarian granulosa cells by targeting RBMS1
    Mianmian Yin
    School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230026, People s Republic of China
    Mol Endocrinol 26:1129-43. 2012
    ..shown that microRNA-383 (miR-383) is one of the most down-regulated miRNA in TGF-β1-treated mouse ovarian granulosa cells (GC)...
  16. ncbi Detection of granulocyte colony-stimulating factor and its receptor in human follicular luteinized granulosa cells
    Ali Salmassi
    Department of Obstetrics and Gynecology, University of Kiel, Michaelisstrasse 16, 24105 Kiel, Germany
    Fertil Steril 81:786-91. 2004
    To evaluate concentration of granulocyte colony-stimulating factor (G-CSF) in serum and follicular fluid (FF) at the time of oocyte retrieval and to detect expression of G-CSF and its receptor by luteinized granulosa cells (GCs).
  17. ncbi Eight-hydroxy-2'-deoxyguanosine in granulosa cells is correlated with the quality of oocytes and embryos in an in vitro fertilization-embryo transfer program
    Tomofumi Seino
    Department of Obstetrics and Gynecology, Yamagata University School of Medicine, Yamagata, Japan
    Fertil Steril 77:1184-90. 2002
    To determine the effects of oxidative stress on the quality of oocytes and embryos, 8-hydroxy-2'-deoxyguanosine (8-OHdG) in granulosa cells was quantitatively studied during an in vitro fertilization and embryo transfer (IVF-ET) program.
  18. ncbi Changes in expression of bone morphogenetic proteins (BMPs), their receptors and inhibin co-receptor betaglycan during bovine antral follicle development: inhibin can antagonize the suppressive effect of BMPs on thecal androgen production
    Claire Glister
    School of Biological Sciences, Hopkins Building, University of Reading, Whiteknights, Reading RG6 6UB, UK
    Reproduction 140:699-712. 2010
    ....
  19. ncbi Human cumulus granulosa cell gene expression: a predictor of fertilization and embryo selection in women undergoing IVF
    L J McKenzie
    Department of Obstetrics, Baylor College of Medicine, Houston, TX 77030, USA
    Hum Reprod 19:2869-74. 2004
    ....
  20. ncbi Notch signaling is involved in ovarian follicle development by regulating granulosa cell proliferation
    Chun Ping Zhang
    State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China
    Endocrinology 152:2437-47. 2011
    ..that the cultured follicles completely stopped developing after L-658,458 and DAPT treatment, most of the granulosa cells were detached, and the oocytes were also degenerated with condensed cytoplasma...
  21. ncbi Highly prolific Booroola sheep have a mutation in the intracellular kinase domain of bone morphogenetic protein IB receptor (ALK-6) that is expressed in both oocytes and granulosa cells
    T Wilson
    AgResearch Molecular Biology Unit, Department of Biochemistry, University of Otago, Dunedin, New Zealand
    Biol Reprod 64:1225-35. 2001
    ..The mutation in BMPR-IB found in Booroola sheep is the second reported defect in a gene from the TGF-beta pathway affecting fertility in sheep following the recent discovery of mutations in the growth factor, GDF9b/BMP15...
  22. ncbi In human granulosa cells from small antral follicles, androgen receptor mRNA and androgen levels in follicular fluid correlate with FSH receptor mRNA
    M E Nielsen
    The Fertility Clinic, Odense University Hospital, Odense, Denmark
    Mol Hum Reprod 17:63-70. 2011
    ..From the individual aspirated follicles, granulosa cells and the corresponding follicular fluid were isolated in 64 follicles, of which 55 were available for mRNA ..
  23. pmc Impairing follicle-stimulating hormone (FSH) signaling in vivo: targeted disruption of the FSH receptor leads to aberrant gametogenesis and hormonal imbalance
    A Dierich
    Institut de Genetique et de Biologie Moleculaire et Cellulaire, Centre National de la Recherche Scientifique, 163, 67404 Illkirch, Strasbourg, France
    Proc Natl Acad Sci U S A 95:13612-7. 1998
    ..FSH receptors (FSH-Rs) are localized to testicular Sertoli cells and ovarian granulosa cells and are coupled to activation of the adenylyl cyclase and other signaling pathways...
  24. ncbi Metformin has direct effects on human ovarian steroidogenesis
    Rebecca Mansfield
    St George s Hospital Medical School, Tooting, London, United Kingdom
    Fertil Steril 79:956-62. 2003
    ..To investigate the possibility of direct effects of metformin on ovarian steroidogenesis...
  25. ncbi The proximal promoter region of the zebrafish gsdf gene is sufficient to mimic the spatio-temporal expression pattern of the endogenous gene in Sertoli and granulosa cells
    Aude Gautier
    INRA, UR1037 SCRIBE, Testicular Physiology and Puberty Research Team, IFR140, Ouest BioGenOuest, Rennes, France
    Biol Reprod 85:1240-51. 2011
    ..Gene expression is maintained in adult granulosa cells and Sertoli cells but decreases in the cells that are in contact with meiotic and postmeiotic germ cells...
  26. pmc Impaired follicle development and infertility in female mice lacking steroidogenic factor 1 in ovarian granulosa cells
    Carla Pelusi
    Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, Texas 75390 8857, USA
    Biol Reprod 79:1074-83. 2008
    ..Herein, we have extended our analyses of knockout (KO) mice with markedly decreased SF-1 expression in granulosa cells. As described, these SF-1 KO mice had hypoplastic ovaries that contained a decreased number of follicles and ..
  27. pmc Peroxisome proliferator-activated receptor-gamma mediates bisphenol A inhibition of FSH-stimulated IGF-1, aromatase, and estradiol in human granulosa cells
    Jakub Kwintkiewicz
    Department of Obstetrics, Gynecology and Reproductive Sciences, University of California San Francisco, San Francisco, California, USA
    Environ Health Perspect 118:400-6. 2010
    ..Bisphenol A (BPA), a chemical used as a plasticizer, is a potent endocrine disruptor that, even in low concentrations, disturbs normal development and functions of reproductive organs in different species...
  28. ncbi Dynamics of the membrana granulosa during expansion of the ovarian follicular antrum
    R J Rodgers
    Department of Medicine, Flinders University of South Australia, Bedford Park, SA 5042, Australia
    Mol Cell Endocrinol 171:41-8. 2001
    ..Here, we focus on the granulosa cells of ovarian follicles which express 17beta-hydroxysteroid dehydrogenase type 1 and cytochrome P450 aromatase...
  29. ncbi Expression of LRH-1 and SF-1 in the mouse ovary: localization in different cell types correlates with differing function
    Margaret M Hinshelwood
    Department of Obstetrics and Gynecology, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, TX, 75390 9038, USA
    Mol Cell Endocrinol 207:39-45. 2003
    ..LRH-1 expression was found to be abundant and highly restricted to cells involved in estrogen biosynthesis-granulosa cells during the estrous cycle, and in corpora lutea (CL) of pregnancy...
  30. ncbi Human ovarian follicular development: from activation of resting follicles to preovulatory maturation
    A Gougeon
    INSERM U865, Anipath, Faculte de Medecine Laennec, 7, Rue Guillaume Paradin, 69372 Lyon Cedex 08, France
    Ann Endocrinol (Paris) 71:132-43. 2010
    ..by subtle interactions between follicle-stimulating hormone (FSH) and local factors produced by theca and granulosa cells (GCs), as well as the oocyte...
  31. ncbi T-2 toxin induces apoptosis in ovarian granulosa cells of rats through reactive oxygen species-mediated mitochondrial pathway
    Jing Wu
    Evaluation and Research Center for Toxicology, Institute of Disease Control and Prevention, Academy of Military Medical Sciences, 20 Dongdajie Street, Fengtai District, Beijing 100071, PR China
    Toxicol Lett 202:168-77. 2011
    ..To investigate the reproductive toxicity and cytotoxicity of T-2 toxin, which is a mycotoxin, and to explore its potential apoptotic induction mechanism...
  32. ncbi Opposing effects of glutathione depletion and follicle-stimulating hormone on reactive oxygen species and apoptosis in cultured preovulatory rat follicles
    Miyun Tsai-Turton
    Center for Occupational and Environmental Health, University of California Irvine, 5201 California Avenue, Suite 100, Irvine, California 92617, USA
    Endocrinology 147:1224-36. 2006
    ..BSO suppressed GSH to undetectable levels. Treatment with FSH prevented apoptosis in granulosa cells, measured by terminal dUTP transferase-mediated nick-end-labeling and activated caspase 3 ..
  33. ncbi GnRH antagonist cetrorelix inhibits mitochondria-dependent apoptosis triggered by chemotherapy in granulosa cells of rats
    Xue jing Zhao
    Department of Obstetrics and Gynecology, Xi Jing Hospital, 4th Military Medical University, Xi an, Shaanxi 710033, PR China
    Gynecol Oncol 118:69-75. 2010
    ..this study were to examine the role of the cetrorelix for prevention of mitochondria-dependent apoptosis in granulosa cells of rats during the treatment with cyclophosphamide(Cy), if the mitochondria-dependent apoptotic process was ..
  34. ncbi Role of transforming growth factor-beta1 in gene expression and activity of estradiol and progesterone-generating enzymes in FSH-stimulated bovine granulosa cells
    Xiaofeng Zheng
    Faculte de Medecine Veterinaire, Centre de Recherche en Reproduction Animale CRRA, Universite de Montreal, St Hyacinthe, Quebec, Canada
    Reproduction 136:447-57. 2008
    ..in the regulation of estradiol-17beta (E(2)) and progesterone (P(4)) secretion in FSH-stimulated bovine granulosa cells. Granulosa cells were obtained from 2 to 5 mm follicles and cultured in serum-free medium...
  35. ncbi Influence of lysophosphatidic acid on estradiol production and follicle stimulating hormone action in bovine granulosa cells
    Dorota Boruszewska
    Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, Poland
    Reprod Biol 13:344-7. 2013
    ..acid (LPA) on 17β-estradiol (E2) synthesis and follicle stimulating hormone (FSH) action in bovine granulosa cells. We found that granulosa cells in the bovine antral follicle, in addition to the uterus and the CL, are also ..
  36. ncbi Expression of IGF-II mRNA-binding proteins (IMPs) in gonads and testicular cancer
    Niels A Hammer
    Department of Clinical Biochemistry, Rigshospitalet, Copenhagen, Denmark
    Reproduction 130:203-12. 2005
    ..mature mouse and human ovaries, IMP1, IMP2 and IMP3 were detected in resting and growing oocytes and in the granulosa cells. In testis, IMP1 and IMP3 were found mainly in the spermatogonia, whereas IMP2 was expressed in the immature ..
  37. ncbi Cellular basis for paracrine regulation of ovarian follicle development
    D F Albertini
    Department of Anatomy and Cellular Biology, Tufts University School of Medicine, Boston, MA 02111, USA
    Reproduction 121:647-53. 2001
    ..have been identified that appear to exert important effects on the growth and differentiation of oocytes and granulosa cells. This article summarizes evidence to support a model for bi-directional paracrine communication that is based ..
  38. ncbi Morphological classification of bovine ovarian follicles
    R J Rodgers
    The Robinson Institute, Research Centre for Reproductive Health, Discipline of Obstetrics and Gynaecology, University of Adelaide, Adelaide, South Australia 5005, Australia
    Reproduction 139:309-18. 2010
    ..primordial follicles have the classical primordial shape, but ellipsoidal shaped follicles with some cuboidal granulosa cells at the poles are far more common...
  39. pmc Mouse oocyte control of granulosa cell development and function: paracrine regulation of cumulus cell metabolism
    You Qiang Su
    The Jackson Laboratory, Bar Harbor, Maine 04609, USA
    Semin Reprod Med 27:32-42. 2009
    Bidirectional communication between oocytes and the companion granulosa cells is essential for the development and functions of both compartments...
  40. ncbi Concentration of activin A and follistatin in follicular fluid from human small antral follicles associated to gene expression of the corresponding granulosa cells
    J V Jeppesen
    Laboratory of Reproductive Biology, The Juliane Marie Centre for Women, Children, and Reproduction Copenhagen University Hospital, Copenhagen University, Denmark
    Mol Cell Endocrinol 356:48-54. 2012
    ..FSHR), LH-receptor (LHR), AMH-receptor2 (AMHR2), CYP19a, and androgen-receptor (AR) in the corresponding granulosa cells (GC)...
  41. pmc Transcriptome profiling of sheep granulosa cells and oocytes during early follicular development obtained by laser capture microdissection
    Agnes Bonnet
    INRA, UMR444 Génétique Cellulaire, Auzeville, Castanet Tolosan, France
    BMC Genomics 12:417. 2011
    ..regulated by cell-cell interactions and by the coordinated expression of genes in both the oocyte and in granulosa cells. Despite many studies, little is known about the cell-specific gene expression driving early folliculogenesis...
  42. ncbi Research resource: small RNA-seq of human granulosa cells reveals miRNAs in FSHR and aromatase genes
    Agne Velthut-Meikas
    Competence Centre on Reproductive Medicine and Biology, Tiigi 61b, 50410 Tartu, Estonia
    Mol Endocrinol 27:1128-41. 2013
    The granulosa cells in the mammalian ovarian follicle respond to gonadotropin signaling and are involved in the processes of folliculogenesis and oocyte maturation...
  43. pmc Glycomic analyses of ovarian follicles during development and atresia
    Nicholas Hatzirodos
    Research Centre for Reproductive Health, Discipline of Obstetrics and Gynaecology, School of Paediatrics and Reproductive Health, Robinson Institute, University of Adelaide, SA, 5005, Australia
    Matrix Biol 31:45-56. 2012
    ..development and atresia, the specialized stromal theca layers were separated from the stratified epithelial granulosa cells of healthy (n=6) and atretic (n=6) follicles in each of three size ranges: small (3-5mm), medium (6-9mm) and ..
  44. ncbi Follicle-stimulating hormone regulation of estradiol production: possible involvement of WNT2 and β-catenin in bovine granulosa cells
    B I Castañon
    Department of Animal Science, Oklahoma State University, Stillwater, OK 74078, USA
    J Anim Sci 90:3789-97. 2012
    ..tumor virus integration site (WNT)/CTNNB1 pathway components in bovine folliculogenesis and steroidogenesis, granulosa cells and follicular fluid were collected from large antral follicles (8 to 22 mm) from ovaries containing stage-..
  45. ncbi Functional oxytocin receptors in bovine granulosa cells
    K Okuda
    Division of Animal Science and Technology, Faculty of Agriculture, Okayama University, Japan
    Biol Reprod 56:625-31. 1997
    The aim of this study was to identify the presence of functional oxytocin (OT) receptors on bovine granulosa cells. Freshly prepared bovine granulosa cells from small (3-5 mm in diameter) or preovulatory (mature) follicles were examined ..
  46. pmc The role for runt related transcription factor 2 (RUNX2) as a transcriptional repressor in luteinizing granulosa cells
    Eun Sil Park
    Department of Obstetrics and Gynecology, Chandler Medical Center, University of Kentucky, Lexington, KY 40536 0298, USA
    Mol Cell Endocrinol 362:165-75. 2012
    ..role of RUNX2 transcription factor in regulating the expression of Runx1, Ptgs2, and Tnfaip6 using cultured granulosa cells isolated from PMSG-primed immature rats...
  47. pmc Effect of apoptosis and reactive oxygen species production in human granulosa cells on oocyte fertilization and blastocyst development
    Nina Jancar
    Reproductive Unit, Department of Obstetrics and Gynecology, University Medical Centre Ljubljana, Slajmerjeva 3, SI 1000 Ljubljana, Slovenia
    J Assist Reprod Genet 24:91-7. 2007
    ..The aim was to establish the impact of human granulosa cell apoptosis and reactive oxygen species (ROS) production on fertilization competence of the oocyte, embryo developmental stage and implantation rate...
  48. pmc CCAAT/enhancer-binding proteins (C/EBP)-α and -β are essential for ovulation, luteinization, and the expression of key target genes
    Heng Yu Fan
    Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas 77030, USA
    Mol Endocrinol 25:253-68. 2011
    ..Thus, C/EBPα/β mediate the terminal differentiation of GCs during the complex process of luteinization...
  49. ncbi Anti-Müllerian hormone expression pattern in the human ovary: potential implications for initial and cyclic follicle recruitment
    Christien Weenen
    Department of Obstetrics and Gynaecology, Erasmus MC, The Netherlands
    Mol Hum Reprod 10:77-83. 2004
    ..observed in primordial follicles, whereas 74% of the primary follicles showed at least a weak signal in the granulosa cells. The highest level of AMH expression was present in the granulosa cells of secondary, preantral and small ..
  50. ncbi Doxorubicin-induced apoptosis in germinal vesicle (GV) oocytes
    Hadas Bar-Joseph
    Department of Cell and Developmental Biology, Sackler Faculty of Medicine, Tel Aviv University, Ramat Aviv, Tel Aviv, Israel
    Reprod Toxicol 30:566-72. 2010
    ..We demonstrated that DXR crosses the blood-follicle barrier and accumulates in oocytes and granulosa cells. The mechanism of DXR-induced apoptosis involves chromosomal disintegration, activation of the mitochondria ..
  51. ncbi Oocyte-derived BMP15 and FGFs cooperate to promote glycolysis in cumulus cells
    Koji Sugiura
    The Jackson Laboratory, Bar Harbor, ME 04609, USA
    Development 134:2593-603. 2007
    ..Therefore, oocyte-derived BMP15 and FGFs cooperate to promote glycolysis in cumulus cells...
  52. ncbi Nodal signalling and apoptosis
    Hongmei Wang
    State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China
    Reproduction 133:847-53. 2007
    ..Studies of Nodal in adult tissues indicate that it is pro-apoptotic in rat ovarian granulosa cells, human trophoblast cells and human ovarian epithelial cancer cells and is growth inhibitory in the latter two ..
  53. ncbi The imitation switch protein SNF2L regulates steroidogenic acute regulatory protein expression during terminal differentiation of ovarian granulosa cells
    Maribeth A Lazzaro
    Molecular Medicine Program, Ottawa Health Research Institute, Ottawa, Ontario, Canada K1H 8L6
    Mol Endocrinol 20:2406-17. 2006
    ..that promotes ovulation and development of the corpus luteum through terminal differentiation of granulosa cells. The pronounced expression of the mammalian imitation switch (ISWI) genes, SNF2H and SNF2L, in adult ovaries ..
  54. pmc Prohibitin (PHB) acts as a potent survival factor against ceramide induced apoptosis in rat granulosa cells
    Indrajit Chowdhury
    Department of Obstetrics and Gynecology, Morehouse School of Medicine, Atlanta, GA, USA
    Life Sci 89:295-303. 2011
    ..Our previous studies have shown that Prohibitin (PHB) is intimately involved in GCs differentiation, atresia, and luteolysis...
  55. pmc RUNX2 transcription factor regulates gene expression in luteinizing granulosa cells of rat ovaries
    Eun Sil Park
    Department of Obstetrics and Gynecology, Chandler Medical Center, University of Kentucky, Lexington, Kentucky 40536 0298, USA
    Mol Endocrinol 24:846-58. 2010
    ..Runx2 expression was induced in periovulatory granulosa cells of human and rodent ovaries...
  56. pmc Follicle-stimulating hormone activation of hypoxia-inducible factor-1 by the phosphatidylinositol 3-kinase/AKT/Ras homolog enriched in brain (Rheb)/mammalian target of rapamycin (mTOR) pathway is necessary for induction of select protein markers of follic
    Hena Alam
    Department of Cell and Molecular Biology, Northwestern University, Feinberg School of Medicine, Chicago, IL 60611, USA
    J Biol Chem 279:19431-40. 2004
    ..These results show that FSH enhances HIF-1 activity downstream of the PI 3-kinase/AKT/Rheb/mTOR pathway in GCs and that HIF-1 activity is necessary for FSH to induce multiple follicular differentiation markers...
  57. pmc Gonadotropin stimulation of ovarian fractalkine expression and fractalkine augmentation of progesterone biosynthesis by luteinizing granulosa cells
    Ping Zhao
    Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, People s Republic of China
    Endocrinology 149:2782-9. 2008
    ..induction of fractalkine transcripts in different ovarian compartments, with the highest increases found in granulosa cells. In cultured granulosa cells, treatment with fractalkine augmented hCG stimulation of progesterone but not ..
  58. ncbi Production of cloned calves following nuclear transfer with cultured adult mural granulosa cells
    D N Wells
    AgResearch, Ruakura Research Centre, PB 3123, Hamilton, New Zealand
    Biol Reprod 60:996-1005. 1999
    Adult somatic cell nuclear transfer was used to determine the totipotent potential of cultured mural granulosa cells, obtained from a Friesian dairy cow of high genetic merit...
  59. ncbi Gap junctions and ovarian folliculogenesis
    Gerald M Kidder
    Department of Physiology, The University of Western Ontario, London, N6A 5C1, Canada
    Reproduction 123:613-20. 2002
    ..Connexin37 appears to be the only connexin contributed by oocytes to the gap junctions coupling them with granulosa cells, and loss of this connexin interferes with the development of antral follicles...
  60. ncbi Hormone-induced proliferation and differentiation of granulosa cells: a coordinated balance of the cell cycle regulators cyclin D2 and p27Kip1
    R L Robker
    Department of Cell Biology, Baylor College of Medicine, Houston, Texas 77030, USA
    Mol Endocrinol 12:924-40. 1998
    The proliferation and terminal differentiation of granulosa cells are critical for normal follicular growth, ovulation, and luteinization...
  61. ncbi Melatonin and the ovary: physiological and pathophysiological implications
    Hiroshi Tamura
    Department of Cellular and Structural Biology, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, TX 78229 3900, USA
    Fertil Steril 92:328-43. 2009
    ..To summarize the role of melatonin in the physiology and pathophysiology of the ovary...
  62. pmc FSH signaling pathways in immature granulosa cells that regulate target gene expression: branching out from protein kinase A
    Mary Hunzicker-Dunn
    Department of Cell and Molecular Biology, Northwestern University Feinberg School of Medicine, Chicago, IL 60611, USA
    Cell Signal 18:1351-9. 2006
    ..FSH accomplishes these actions by inducing a complex pattern of gene expression in target granulosa cells that is regulated by input from many different signaling cascades, including those for the extracellular ..
  63. pmc Induction of cyclin D2 in rat granulosa cells requires FSH-dependent relief from FOXO1 repression coupled with positive signals from Smad
    Youngkyu Park
    Department of Cell and Molecular Biology, Northwestern University Feinberg School of Medicine, Chicago, Illinois 60611, USA
    J Biol Chem 280:9135-48. 2005
    ..growth in response to follicle-stimulating hormone (FSH), largely as a result of the proliferation of granulosa cells (GCs)...
  64. pmc Hypergonadotropic ovarian failure associated with an inherited mutation of human bone morphogenetic protein-15 (BMP15) gene
    Elisa Di Pasquale
    Institute of Endocrine Sciences, University of Milan, and Istituto Auxologico Italiano IRCCS, Milan, Italy
    Am J Hum Genet 75:106-11. 2004
    ..BMP15 defects are involved in the pathogenesis of hypergonadotropic ovarian failure in humans...
  65. pmc A negative feedback system between oocyte bone morphogenetic protein 15 and granulosa cell kit ligand: its role in regulating granulosa cell mitosis
    Fumio Otsuka
    Department of Reproductive Medicine, School of Medicine, University of California at San Diego, La Jolla, CA 92093 0633, USA
    Proc Natl Acad Sci U S A 99:8060-5. 2002
    ..We hypothesize that the negative feedback system most likely plays a pivotal role in early folliculogenesis...
  66. ncbi The regulation of ovarian granulosa cell death by pro- and anti-apoptotic molecules
    Fuko Matsuda-Minehata
    Research Unit for Animal Life Sciences, Animal Resource Science Center, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Ibaraki Kasama, Japan
    J Reprod Dev 52:695-705. 2006
    ..Recent studies suggest that the apoptosis of ovarian granulosa cells plays a major role in follicular atresia...
  67. pmc Targeted disruption of Pten in ovarian granulosa cells enhances ovulation and extends the life span of luteal cells
    Heng Yu Fan
    Department of Molecular and Cellular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA
    Mol Endocrinol 22:2128-40. 2008
    ..To selectively disrupt Pten expression in granulosa cells, Ptenfl/fl mice were mated with transgenic mice expressing cAMP response element recombinase driven by Cyp19 ..
  68. pmc Liver receptor homolog 1 is essential for ovulation
    Rajesha Duggavathi
    Institut de Genetique et de Biologie Moleculaire et Cellulaire, CNRS INSERM ULP, 67404 Illkirch, France
    Genes Dev 22:1871-6. 2008
    ..Using somatic targeted mutagenesis, we show that mice lacking Lrh1 in granulosa cells are sterile, due to anovulation...
  69. ncbi Effects of metformin on bovine granulosa cells steroidogenesis: possible involvement of adenosine 5' monophosphate-activated protein kinase (AMPK)
    Lucie Tosca
    Unite de Physiologie de la Reproduction et des Comportements, Institut National de la Recherche Agronomique, 37380 Nouzilly, France
    Biol Reprod 76:368-78. 2007
    ..In granulosa cells from small follicles, metformin (10 mM) reduced production of both progesterone and estradiol and decreased ..
  70. ncbi Luteinizing hormone receptor, steroidogenesis acute regulatory protein, and steroidogenic enzyme messenger ribonucleic acids are overexpressed in thecal and granulosa cells from polycystic ovaries
    A J Jakimiuk
    Department of Obstetrics and Gynecology, CSMC Burns and Allen Research Institute, University of California School of Medicine, Los Angeles, California 90048 0750, USA
    J Clin Endocrinol Metab 86:1318-23. 2001
    ..acids (mRNAs) may be overexpressed in thecal cells, and LH receptors may be prematurely expressed in granulosa cells in women with polycystic ovaries...
  71. ncbi Regulation of ovarian function: the role of anti-Müllerian hormone
    Alexandra L L Durlinger
    Department of Internal Medicine, Erasmus MC, PO Box 1738, 3000 DR Rotterdam, The Netherlands
    Reproduction 124:601-9. 2002
    ....
  72. pmc Loss of GATA-6 and GATA-4 in granulosa cells blocks folliculogenesis, ovulation, and follicle stimulating hormone receptor expression leading to female infertility
    Jill Bennett
    Department of Physiology and Biophysics, College of Medicine, University of Illinois at Chicago, Chicago, Illinois 60612, USA
    Endocrinology 153:2474-85. 2012
    ..Most prominently, FSH receptor (FSHR) protein was undetectable in granulosa cells of G4(gcko) and G4/6(gcko)...
  73. pmc Progesterone receptor membrane component-1 (PGRMC1) is the mediator of progesterone's antiapoptotic action in spontaneously immortalized granulosa cells as revealed by PGRMC1 small interfering ribonucleic acid treatment and functional analysis of PGRMC1 m
    John J Peluso
    Department of Cell Biology, University of Connecticut Health Center, Farmington, CT 06030, USA
    Endocrinology 149:534-43. 2008
    ..Additional studies are required to more precisely delineate the role of each PGRMC1 domain in transducing P4's antiapoptotic action...
  74. ncbi Expression of hyaluronan synthases and corresponding hyaluronan receptors is differentially regulated during oocyte maturation in cattle
    Martin Schoenfelder
    Institute of Physiology, Technical University of Munich, D 85354 Freising, Germany
    Biol Reprod 69:269-77. 2003
    ..determined the histological localization of HA and its receptor, CD44, in maturing bovine COCs and cultured granulosa cells (GCs). Full-length transcript of bovine HAS2 and a part of the bovine RHAMM sequence has been made available...
  75. ncbi Immunohistochemical visualization of insulin receptors in formalin-fixed bovine ovaries post mortem and in granulosa cells collected in vivo
    P Bossaert
    Department of Reproduction, Obstetrics and Herd Health, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium
    Theriogenology 73:1210-9. 2010
    ..Furthermore, we have developed a quantifiable staining technique for bovine follicles that may be applicable for GC obtained in live cows, although this method requires further standardization...
  76. ncbi Altered composition of the cumulus-oocyte complex matrix during in vitro maturation of oocytes
    Kylie R Dunning
    Discipline of Obstetrics and Gynaecology, Research Centre for Reproductive Health, The University of Adelaide, Adelaide, SA, Australia
    Hum Reprod 22:2842-50. 2007
    ..identified two extracellular matrix proteins, the protease Adamts1 and hyaluronan-binding proteoglycan Versican, produced by mural granulosa cells that selectively incorporate into the periovulatory cumulus-oocyte complex (COC).
  77. ncbi Ultrastructural characterization of mouse embryonic stem cell-derived oocytes and granulosa cells
    Olympia E Psathaki
    Department of Cell and Developmental Biology, Max Planck Institute for Molecular Biomedicine, Munster, Germany
    Stem Cells Dev 20:2205-15. 2011
    ..We have generated putative oocytes and granulosa cells in vitro from mouse embryonic stem cells and utilized electron microscopy to characterize these cells...
  78. ncbi Induction of Ad4BP/SF-1, steroidogenic acute regulatory protein, and cytochrome P450scc enzyme system expression in newly established human granulosa cell lines
    K Hosokawa
    Department of Molecular Cell Biology, The Weizmann Institute of Science, Rehovot, Israel
    Endocrinology 139:4679-87. 1998
    We have established immortalized human granulosa cells by triple transfection of primary cells obtained from in vitro fertilization patients with SV40 DNA, Ha-ras oncogene, and a temperature sensitive (ts) mutant of the tumor suppressor ..
  79. ncbi CREB coactivator CRTC2/TORC2 and its regulator calcineurin crucially mediate follicle-stimulating hormone and transforming growth factor β1 upregulation of steroidogenesis
    Wei Ling Fang
    Institute of Physiology, School of Medicine, National Yang Ming University, Taipei, Taiwan
    J Cell Physiol 227:2430-40. 2012
    ..Primary culture of granulosa cells from gonadotropin-primed immature rats was used...
  80. ncbi Oocyte-somatic cell interactions during follicle development in mammals
    R B Gilchrist
    Research Centre for Reproductive Health, Department of Obstetrics and Gynaecology, University of Adelaide, The Queen Elizabeth Hospital, Woodville, SA 5011, Australia
    Anim Reprod Sci 82:431-46. 2004
    ..The oocyte achieves this, in part, by secreting soluble paracrine growth factors that act on its neighboring granulosa cells, which in turn regulate oocyte development...
  81. pmc Mutation in bone morphogenetic protein receptor-IB is associated with increased ovulation rate in Booroola Mérino ewes
    P Mulsant
    Institut National de la Recherche Agronomique, Laboratoire de Genetique Cellulaire, BP, 27, 31326 Castanet Tolosan, France
    Proc Natl Acad Sci U S A 98:5104-9. 2001
    ..In vitro, ovarian granulosa cells from FecB(B)/FecB(B) ewes were less responsive than granulosa cells from FecB(+)/FecB(+) ewes to the ..
  82. ncbi Activin A stimulates meiotic maturation of human oocytes and modulates granulosa cell steroidogenesis in vitro
    B M Alak
    Department of Obstetrics and Gynecology, The Ohio State University, Columbus 43210, USA
    Fertil Steril 70:1126-30. 1998
    To test the hypothesis that activin A promotes in vitro human oocyte meiotic maturation while inhibiting steroid secretion by nonluteinized antral granulosa cells.
  83. pmc The B cell translocation gene (BTG) family in the rat ovary: hormonal induction, regulation, and impact on cell cycle kinetics
    Feixue Li
    Department of Obstetrics and Gynecology, Chandler Medical Center, University of Kentucky, Lexington, Kentucky 40536 0298, USA
    Endocrinology 150:3894-902. 2009
    ..Ovaries or granulosa cells were collected at various times after hCG administration (n = 3 per time point)...
  84. ncbi Expression and glycosylation with polylactosamine of CD44 antigen on macrophages during follicular atresia in pig ovaries
    Yuko Miyake
    Laboratories of Animal Reproduction, Graduate School of Agricultural Science, Tohoku University, Sendai, 981 8555, Japan
    Biol Reprod 74:501-10. 2006
    ..classified into five stages: stage I, healthy follicles; stage II, early atretic follicles having apoptotic granulosa cells with an unclear basement membrane; stage III, progressing atretic follicles having apoptotic granulosa cells ..
  85. ncbi Bone morphogenetic protein (BMP) ligands and receptors in bovine ovarian follicle cells: actions of BMP-4, -6 and -7 on granulosa cells and differential modulation of Smad-1 phosphorylation by follistatin
    Claire Glister
    School of Animal and Microbial Sciences, University of Reading, Whiteknights, Reading RG6 6AJ, UK
    Reproduction 127:239-54. 2004
    ..Immunocytochemistry revealed expression of BMP-4 and -7 in isolated theca cells whereas granulosa cells and oocytes selectively expressed BMP-6...
  86. ncbi Follicular somatic cell factors and follicle development
    J Buratini
    Departamento de Fisiologia, Universidade Estadual Paulista, Botucatu, Sao Paulo 18618 000, Brasil
    Reprod Fertil Dev 23:32-9. 2011
    Considerable attention is currently paid to oocyte-derived secreted factors that act upon cumulus and granulosa cells. Also important for follicle development are somatic cell-derived secreted factors...
  87. ncbi Quercetin attenuates cadmium-induced oxidative damage and apoptosis in granulosa cells from chicken ovarian follicles
    Yudong Jia
    Key Laboratory of Animal Epidemic Etiology and Immunological Prevention of the Ministry of Agriculture, Department of Veterinary Medicine, College of Animal Sciences, Zhejiang University, No 268 Kaixuan Road, Hangzhou 310029, PR China
    Reprod Toxicol 31:477-85. 2011
    ..effect of quercetin on cadmium-induced oxidative damage and apoptosis was investigated in cultured granulosa cells from chicken ovarian follicles...
  88. pmc Luteinizing hormone receptor mRNA down-regulation is mediated through ERK-dependent induction of RNA binding protein
    Bindu Menon
    Department of Obstetrics Gynecology, University of Michigan Medical School, Ann Arbor, Michigan 48109 0617, USA
    Mol Endocrinol 25:282-90. 2011
    ..The LH-mediated signaling pathways involved in this process were examined in primary cultures of human granulosa cells. Treatment with 10 IU human chorionic gonadotropin (hCG) for 12 h resulted in the down-regulation of LHR mRNA ..
  89. ncbi Oocyte--granulosa cell interactions
    R Canipari
    Dipartimento di Istologia ed Embriologia Medica, University of Rome La Sapienza, Italy
    Hum Reprod Update 6:279-89. 2000
    ..The search for the optimal culture conditions and the correct balance of hormones necessary to obtain a fertilizable oocyte in vitro is extremely important for clinical and agricultural applications...
  90. ncbi Apoptosis in human granulosa cells after induction of ovulation in women participating in an intracytoplasmic sperm injection program
    Ana Clavero
    Servicio de Análisis Cli nicos, Hospital Virgen de las Nieves, Granada, Spain
    Eur J Obstet Gynecol Reprod Biol 110:181-5. 2003
    ....
  91. ncbi Inhibitor of apoptosis proteins and ovarian dysfunction in galactosemic rats
    K W Lai
    Department of Anatomy, The University of Hong Kong, Hong Kong SAR, P R China
    Cell Tissue Res 311:417-25. 2003
    ..Western blot analysis of granulosa cells showed that the overall expressions of Fas and FasL were lower in the control group and their expressions of ..
  92. pmc WNT2 regulates DNA synthesis in mouse granulosa cells through beta-catenin
    Hong Xing Wang
    Departments of Physiology and Pharmacology, Obstetrics and Gynecology, and Pediatrics, Schulich School of Medicine and Dentistry, The University of Western Ontario, London, Ontario, Canada
    Biol Reprod 82:865-75. 2010
    ..the objective of this study was to investigate the regulation and function of WNT2 signaling in mouse granulosa cells. Immunostaining identified WNT2 as being expressed in granulosa cells throughout folliculogenesis, but with ..
  93. pmc Differential granulosa cell gene expression in young women with diminished ovarian reserve
    Keri Greenseid
    Reproductive Endocrinology and Infertility, Department ofObstetrics, Gynecology and Women s Health, Albert Einstein Collegeof Medicine, Bronx, NY, USA
    Reprod Sci 18:892-9. 2011
    To investigate if a diagnosis of diminished ovarian reserve (DOR) is associated with a differential gene profile of ovarian granulosa cells (GCs) in infertile women undergoing in vitro fertilization (IVF).
  94. ncbi Progesterone induces the expression of vascular endothelial growth factor (VEGF) 120 and Flk-1, its receptor, in bovine granulosa cells
    Takashi Shimizu
    Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine Inada Machi, Obihiro, Hokkaido 080 8555, Japan
    Anim Reprod Sci 102:228-37. 2007
    Vascular endothelial growth factor (VEGF) isoforms (VEGF 120 and VEGF 164) secreted by granulosa cells are involved in thecal angiogenesis during follicular development in the bovine ovary...
  95. ncbi What we have learned from isolated cells from human ovary?
    Hitoshi Okamura
    Department of Obstetrics and Gynecology, Kumamoto University School of Medicine, Honjo 1 1 1, Kumamoto 860 8556, Japan
    Mol Cell Endocrinol 202:37-45. 2003
    ..We have recently established the in vitro culture systems of ovarian surface epithelium (OSE) and granulosa cells of humans, and subsequently developed the immortalization of each cell...
  96. pmc A role for nuclear factor interleukin-3 (NFIL3), a critical transcriptional repressor, in down-regulation of periovulatory gene expression
    Feixue Li
    College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, People s Republic of China
    Mol Endocrinol 25:445-59. 2011
    ..with pregnant mare's serum gonadotropin, treated with human chorionic gonadotropin (hCG), and ovaries or granulosa cells were collected at various times after hCG...
  97. ncbi Efficient replication of caprine arthritis-encephalitis virus in goat granulosa cells
    A Lamara
    Laboratoire d Etude des Risques Sanitaires liés aux Biotechnologies de la Reproduction, Ecole Nationale Veterinaire de Nantes, Atlanpole La Chantrerie, B P 40706 44307, Cedex, Nantes, France
    Virus Res 79:165-72. 2001
    ..Since granulosa cells (GC) are of epithelial origin and currently used for in vitro oocyte maturation, we addressed the question ..
  98. ncbi Expression and regulation of transcripts encoding two members of the NR5A nuclear receptor subfamily of orphan nuclear receptors, steroidogenic factor-1 and NR5A2, in equine ovarian cells during the ovulatory process
    D Boerboom
    Centre de Recherche en Reproduction Animale, Faculte de Medecine Veterinaire, Universite de Montreal, Saint Hyacinthe, Quebec, Canada
    Endocrinology 141:4647-56. 2000
    ..caused a significant decrease in SF-1 levels between 12-39 h in theca interna and between 24-39 h post hCG in granulosa cells (P < 0.05)...
  99. ncbi Immunolocalization of the hepatocyte growth factor (HGF) system in the rat ovary and the anti-apoptotic effect of HGF in rat ovarian granulosa cells in vitro
    Mehmet Uzumcu
    Department of Animal Sciences, Rutgers University, New Brunswick, New Jersey 08901, USA
    Reproduction 132:291-9. 2006
    ..Advanced folliculogenesis led to a change in the cellular distribution of the HGF, c-met, and HGFA, suggesting that the ovarian HGF system is hormonally regulated in vivo...
  100. ncbi Inhibin A is a follicle stimulating hormone-responsive marker of granulosa cell differentiation, which has both autocrine and paracrine actions in sheep
    B K Campbell
    Department of Obstetrics and Gynaecology, University of Edinburgh, Centre for Reproductive Biology, 37 Chalmers Street, Edinburgh, UK
    J Endocrinol 169:333-45. 2001
    ..Experiment 2 examined the pattern of inhibin A production, in relation to oestradiol, by granulosa cells induced to differentiate in vitro with follicle-stimulating hormone (FSH)...
  101. ncbi Bisphenol A disrupts granulosa cell function
    F Grasselli
    Dipartimento di Produzioni Animali, Biotecnologie Veterinarie, Qualità e Sicurezza degli Alimenti, Italy
    Domest Anim Endocrinol 39:34-9. 2010
    ..Within the reproductive system, ovarian granulosa cells have been documented as a target of BPA action, but no consensus has been reached about functional ..

Research Grants65

  1. Human Ovarian Steroidogenesis-inducing Protein
    SHAFIQ KHAN; Fiscal Year: 2004
    ..Later studies revealed that SIP is also a potent growth factor and stimulates DNA synthesis in rat granulosa cells, immature rat Leydig cells and human ovarian epithelial cancer cells...
  2. Regulation and Function of the Rhox8 homeobox gene in granulosa cells
    JAMES ARTHUR MACLEAN; Fiscal Year: 2010
    ..The actions of granulosa cells in the ovary are central to successful ovulation...
  3. WNT and gonadotropin mediated regulation of ovarian steroidogenesis
    JENNIFER HERNANDEZ GIFFORD; Fiscal Year: 2010
    ..Moreover, reduction of [unreadable]-catenin in granulosa cells compromises FSH stimulation of aromatase mRNA and resultant estradiol production...
  4. Leukemia Inhibitory Factor As a Mediator of Primate Ovulation &Oocyte Maturation
    Jon D Hennebold; Fiscal Year: 2013
    ..surface LIF binding proteins (LIF receptor, or LIFR;gp130) were further localized to isolated oocytes and granulosa cells. Lastly, in pilot studies, intrafollicula injection of a LIF antagonist (the extracellular LIF binding ..
  5. Regulation of guanylyl cyclase A and B by hormones, ATP and phosphorylation
    Lincoln Ross Potter; Fiscal Year: 2013
    ..Finally, the fourth aim will investigate how hormones inactivate GC- B in mouse follicles, granulosa cells, chondrocytes and smooth muscle cells by assessing the requirements for calcium elevations, PKC activation, ..
  6. Role of mineralocorticoid receptor in primate ovarian function
    CHARLES CHAFFIN; Fiscal Year: 2009
    ..In order to verify an ovarian origin for DOC, granulosa cells were isolated prior to an ovulatory stimulus and cultured in the presence of hCG, resulting in a dramatic ..
  7. Receptor/Ligand Signals for Ovarian Follicular Angiogenesis
    Leon J Spicer; Fiscal Year: 2010
    ..Our overall hypothesis is that granulosa cells signal theca interna via ANG and FGF9 secretion to regulate theca and vascular cell differentiation and ..
  8. DEVELOPMENT OF MAMMALIAN OOCYTE-GRANULOSA CELL COMPLEX
    John J Eppig; Fiscal Year: 2012
    ..In Aim 2, the hypothesis that oocytes control follicular development by affecting how granulosa cells respond to hormonal stimuli will be tested...
  9. Protease regulation of ovarian recrudescence
    KELLY ANSLEY YOUNG; Fiscal Year: 2013
    ..Examination of both mRNA and protein for key markers of angiogenesis (e.g., CD34, VEGF-R1), proliferation of granulosa cells (PCNA), and steroidogenesis (e.g...
  10. Use of a Fragile X premutation knock-in mouse to study FXPOI
    Joshua Johnson; Fiscal Year: 2013
    ..assessment of PM carriers indicates that FSH is elevated, inhibins and anti-Mullerian hormone (both made by granulosa cells) are reduced...
  11. ANALYSIS OF REPRODUCTIVE FUNCTION USING TRANSGENIC MICE
    Martin M Matzuk; Fiscal Year: 2013
    ..pathways in the development of oviductal and uterine smooth muscles;2) Identify the GDF9 type 1 receptor in granulosa cells;3) Determine the molecular aspects for receptor specificity of the BMP15 and GDF9 ligands;and 4) Study the ..
  12. A Novel Function of the Oocyte in Estrogen-Regulated FSH Action
    Shunichi Shimasaki; Fiscal Year: 2009
    ..we have recently discovered that the oocyte is essential for estrogen activity in enhancing FSH action in granulosa cells. We have also found that the synergistic action of estrogen and oocytes occurs at a site downstream of the ..
  13. Functional Significance of the HIF1 Transcriptome in Granulosa Cells
    John H Nilson; Fiscal Year: 2013
    ..provided by applicant): Fertility in females requires controlled maturation of the oocyte, supporting granulosa cells (GCs), and theca cells that comprise the ovarian follicle...
  14. FSH-STIMULATED SIGNALS THAT REGULATE FOLLICULAR MATURATION
    John H Nilson; Fiscal Year: 2013
    ..provided by applicant): Fertility in females requires controlled maturation of the oocyte, supporting granulosa cells (GCs), and thecal cells that comprise the ovarian follicle...
  15. Molecular Pathways Controlling Ovarian Gene Expression
    CARLOS OSCAR STOCCO; Fiscal Year: 2013
    ..Ovarian granulosa cells express high levels of the transcription factors GATA-4 and GATA-6...
  16. MicroRNA Regulation of Ovarian Function
    Lane K Christenson; Fiscal Year: 2013
    ..We have also identified miR-21 as an LH- induced microRNA (miRNA) within granulosa cells of the periovulatory follicle, and demonstrated that it suppresses apoptosis and enhances granulosa cell ..
  17. The Inositol Phosphate Cascade as a Regulator of FSH and LH actions in the Ovary
    Shawn M Breen; Fiscal Year: 2012
    DESCRIPTION (provided by applicant): The gonadotropin-induced expression of aromatase in granulosa cells is dependent on the signaling pathways that are activated, which is dictated by the density of each gonadotropin receptor...
  18. Role of STARD6 in ovarian steroidogenesis
    HOLLY ANNE LAVOIE; Fiscal Year: 2013
    ..Using microarray, we serendipitously found STARD6 mRNA in luteinizing pig granulosa cells and further found it to be highly expressed in midluteal phase corpora lutea, a tissue with high STARD1 ..
  19. Chromium VI-induced toxicity on Female Reproductive Function
    SAKHILA BANU; Fiscal Year: 2012
    ..Our central hypothesis is that lactation exposure to CrVI induces apoptosis of granulosa cells and follicular atresia, decreases ovulation, compromises pregnancy, and induces preterm labor in the F1 ..
  20. Developmental Gene-Environment Interactions and Premature Ovarian Failure
    Ulrike Luderer; Fiscal Year: 2013
    ..2) To delineate the effects of deficient GSH synthesis localized to granulosa cells or oocytes on premature ovarian failure utilizing conditional knockout mouse models with granulosa cell- and ..
  21. Molecular Basis of BMP-15 Function
    Shunichi Shimasaki; Fiscal Year: 2012
    ..Aim 1: Determine the biological function of rhBMP-15 in human granulosa cells at defined stages of follicle development...
  22. Role of the Mullerian Tract in Ovarian Cancer Development
    Louis Dubeau; Fiscal Year: 2013
    ..Dubeau's laboratory that suggests that inactivation of Brca1 in ovarian granulosa cells stimulate neoplastic transformation in tissues that are embryologically derived from the mullerian ducts...
  23. Cyclic AMP Signaling in Granulosa Cells
    Anthony J Zeleznik; Fiscal Year: 2013
    ..Aim 1 will compare the signaling pathways activated by FSH and by PKA-CQR in undifferentiated granulosa cells. Aim 2 will determine which signaling pathways and/or transcription factors interact with PKA to optimally ..
  24. Function of RUNX transcription factors in COCs
    Misung Jo; Fiscal Year: 2013
    ..enzyme in the biosynthesis of prostaglandins) and Hapln1 (a stabilizer of cumulus matrix) in mural granulosa cells and/or cumulus cells. Both gene products are known to play crucial roles in COC expansion...
  25. The Role Of CCL20 In Ovulation: A Novel Mediator Of Ovarian Leukocyte Recruitment
    Thomas E Curry; Fiscal Year: 2013
    ..Aim 3 will investigate the role of CCL20 on ovarian cell function, specifically the ability of granulosa cells to survive, differentiate, and make sterid hormones...
  26. ANALYSIS OF REPRODUCTIVE FUNCTION USING TRANSGENIC MICE
    Martin M Matzuk; Fiscal Year: 2010
    ..of quiescent primordial follicles to primary (one layer) follicles by an unknown mechanism;2) Growth of the granulosa cells of the primary follicle to form two-layered and multi-layered secondary follicles;3) Formation of antral ..
  27. PHYSIOLOGY AND PATHOPHYSIOLOGY OF THE PRIMATE GONAD
    Tony M Plant; Fiscal Year: 2010
    ..At the technical level, methodology developed at the Pittsburgh Center for controlling gene expression in granulosa cells will find direct application to the study of Sertoli cell biology in this proposal...
  28. Role of the BMP co-receptor Dragon (RGMb) in ovarian follicle development
    Herbert Y Lin; Fiscal Year: 2010
    ..Follicle development requires communication between oocytes and granulosa cells. The cellular and molecular processes involved are poorly understood...
  29. PAIRBP &PGRMC1 act as a membrane receptor complex to mediate P4's ovarian action
    John J Peluso; Fiscal Year: 2011
    ..These studies will be conducted on rat granulosa cells, rat luteal and human granulosa/luteal cells...
  30. REGULATION OF THE CORPUS LUTEUM
    Anthony Zeleznik; Fiscal Year: 2003
    ..adenovirus vector that expresses a dominant-negative mutant of CREB will be used to block CREB signaling in granulosa cells. The specific hypothesis is that the loss of CREB- of CREB and PCNA expression...
  31. OVARIAN TUMOR NECROSIS FACTOR
    Paul Terranova; Fiscal Year: 2004
    ..Preliminary data indicate that TNF inhibits FSH and LH stimulated estradiol secretion by granulosa cells in the mouse and human without altering the level of cAMP; this observation is different than that in rat ..
  32. APOPTOSIS AND REPRODUCTIVE AGING OF THE FEMALE
    Jonathan Tilly; Fiscal Year: 2003
    ..a structure composed of a single germ cell (oocyte) surrounded by a somatic cell lineage, referred to as granulosa cells (GC), that is the source of estrogens produced by the ovaries...
  33. FAS AND BC1-2 AND GRANULOSA CELL APOPTOSIS
    Nicholas Cataldo; Fiscal Year: 2000
    ..Oocytes which are not surrounded by granulosa cells nearly i ovarian development undergo atresia, while follicles also undergo atresia at every stage of ..
  34. REGULATION OF MAMMALIAN OOCYTE DEVELOPMENT
    John Eppig; Fiscal Year: 1993
    ..1) Granulosa cells produce hypoxanthine and other purines that could participate in the maintenance of meiotic arrest...
  35. REGULATORY MECHANISMS OF OVARIAN PROGESTERONE RECEPTORS
    Ok Kyong Park Sarge; Fiscal Year: 2000
    ..her postdoctoral training period, that the ovary transiently expresses the progesterone receptor gene in granulosa cells of preovulatory follicles during the period critical for ovulatory processes, clearly suggests that the ovary ..
  36. HORMONE ACTION IN THE OVARY: ROLE OF CALCIUM IONS
    Johannes Veldhuis; Fiscal Year: 1999
    ..Based on this background, in this renewal proposal we will use primary cultures of untransformed swine granulosa cells to investigate: (i) the regulated biochemical mechanisms that govern the distinctive FSH versus LH- promoted [..
  37. ACTIONS OF ESTROGEN IN MATURING GRAAFIAN FOLLICLES
    Johannes Veldhuis; Fiscal Year: 2005
    ..b>Granulosa cells in the dominant Graafian follide undergo progressive steroidogenic cytodifferentiation in preparation for the ..
  38. The function(s) and regulation of PPARs in the ovary
    Carolyn Komar; Fiscal Year: 2003
    ..She has preliminary data showing that PPAR gamma expression is restricted to granulosa cells and is down regulated by the gonadotropin surge...
  39. REGULATION OF FOLLITROPIN ACTIONS
    Mario Ascoli; Fiscal Year: 2009
    ..The LH and FSH receptors (LHR and FSHR, respectively) are both expressed in granulosa cells but in spite of structural and functional similarities between LH and FSH and between the LHR and the FSHR, ..
  40. REGULATION OF MIS TYPE II RECEPTOR AND TARGET GENES
    Jose Teixeira; Fiscal Year: 2002
    ..MIS is also produced both in the adult testis by Sertoli cells and in the ovary by granulosa cells, where its exact role remains to be fully explored...
  41. AROMATASE IN ADIPOSE--RELATIONSHIP TO AGING AND CANCER
    Evan Simpson; Fiscal Year: 2005
    In the human, estrogens are synthesized in a number of tissue sites including ovarian granulosa cells and corpus luteum, placental syncytiotrophoblast, various sites in the brain, as well as adipose tissue...
  42. RECEPTOR MECHANISMS OF IGF-II ACTION IN THE OVARY
    Leonid Poretsky; Fiscal Year: 1999
    ..the IGF-II induced suppression of insulin-like growth factor binding protein I (IGFBP-1) production in human granulosa cells. The following hypotheses will be tested: 1...
  43. MESENCHYMAL/EPITHELIAL CELL INTERACTIONS IN THE OVARY
    Michael Skinner; Fiscal Year: 2000
    ..Emphasis is placed on the elucidation of mesenchymal-epithelial cell interactions between theca cells and granulosa cells. Previous research and preliminary studies have demonstrated that the mesenchymal derived theca cells produce ..
  44. Ovarian Aspects of Caloric Restriction
    MARY ZELINSKI WOOTEN; Fiscal Year: 2004
    ..e. granulosa cells, to exogenous gonadotropin or "fertility" treatment, and resultant follicular growth and maturation; and 3) ..
  45. Ovarian Primordial Follicle Development
    Michael K Skinner; Fiscal Year: 2010
    ..to be mediated by the local production and action of specific paracrine factors involving theca cells, granulosa cells, and the oocyte. Preliminary studies have shown that steroid hormones (i.e...
  46. OOCYTE CULTURE CONDITIONS THAT PROMOTE EMBRYOGENESIS
    John Eppig; Fiscal Year: 1999
    ..that improvement of the development of oocytes in vitro is dependent upon (1) suppressing apoptosis in granulosa cells and (2) promoting the appropriate differentiation of the granulosa cells coupled to the oocytes...
  47. PEPTIDE GROWTH FACTORS IN POLYCYSTIC OVARIAN DISEASE
    John Yeh; Fiscal Year: 1992
    ..developed a rat model for polycystic ovary in which the in vitro and in vivo effects of hyperandrogenism on granulosa cells can be tested experimentally...
  48. CELL NON-AUTONOMOUS MODE OF TUMOR SUPPRESSION BY BRCA1
    Louis Dubeau; Fiscal Year: 2009
    ..This led us to hypothesize that BRCA1 might predispose to ovarian cancer indirectly, by influencing ovarian granulosa cells, which play an important role in controlling menstrual cycle progression through their secretion of sex ..
  49. REGULATION OF R55 EXPRESSION IN EARLY FOLLICULOGENESIS
    Vaughan Lee; Fiscal Year: 2002
    ..in oocytes of activated primordial follicles, while expression of FSH receptors and inhibin alpha occurs in granulosa cells during the first stages of morphological differentiation...
  50. COORDINATION OF FOLLICULOGENESIS AND OOGENESIS
    David Albertini; Fiscal Year: 2007
    ..is that GDF-9 and FSH exert antagonistic influences on an epithelial to mesenchymal transition (EMT) in granulosa cells (GCs)...
  51. NOTCH SIGNALING REGULATES GRANULOSA CELL DIFFERENTIATION
    NORMA WILSON RAWLS; Fiscal Year: 2002
    ..regulates cell fate decisions between adjacent cells and otch pathway genes were found to be expressed in granulosa cells of developing follicles...
  52. WATER TRAFFICKING IN THE OVARY
    FRANCIS HUGHES; Fiscal Year: 2002
    In the mammalian ovary, a follicle consists of a single oocyte surrounded by a layer of granulosa cells, a basement membrane and a third cell type known as theca cells...
  53. IGFS AS REGULATORS OF ENDOCRINE CELL GROWTH & FUNCTION
    MITCHELL RABKIN; Fiscal Year: 1990
    ..FRTL5, that maintains thyroid differentiated function in vitro and the use of freshly obtained human ovarian granulosa cells. The goals of the project will be to: 1) identify the cell-surface membrane receptors for IGF-I, IGF-II, and ..
  54. GRANULOSA CELL FUNCTION DURING FOLLICULOGENESIS
    Virendra Mahesh; Fiscal Year: 1993
    The growth and development of ovarian follicles is dependent upon the population of granulosa cells present in the follicle...
  55. OOCYTE CULTURE CONDITIONS THAT PROMOTE EMBRYOGENESIS
    John Eppig; Fiscal Year: 2009
    ..embryogenesis and long-term health of offspring and (2) explore the communication between oocytes and granulosa cells and its impact on oocyte development...
  56. REGULATION OF GRANULOSA CELL FSH RECEPTOR
    Andrew La Barbera; Fiscal Year: 2002
    It is important that we understand how follicle-stimulating hormone (FSH) regulates its receptor in ovarian granulosa cells become hormone analogs and drugs that affect follicular development via the FSH receptor (FSHR) are being ..
  57. Mechanisms of p53 action on primate steroidogenesis
    CHARLES CHAFFIN; Fiscal Year: 2005
    ..protein beta (CEBPb), we have recently discovered that the initial increase in steroid synthesis by primate granulosa cells depends upon the tumor suppressor p53...
  58. SF1 & CREB: GONADAL ROLES BY A NEW TRANSGENIC APPROACH
    LESLIE HECKERT; Fiscal Year: 1999
    Sertoli and granulosa cells form an important somatic component of the gonads. These cells help regulate germ cell development by providing essential nutrients and regulatory signals that support their maturation...
  59. BIOLOGICAL BASIS OF THE PERIMENOPAUSE
    DAVID SEIFER; Fiscal Year: 2002
    ..This project is directed at understanding the biological basis of this transition by investigating granulosa cells which are fundamental components of the aging follicle...
  60. WATER MOVEMENT DURING CELL DEATH
    FRANCIS HUGHES; Fiscal Year: 2002
    ..oocytes are lost in a process termed atresia which is characterized by massive apoptosis of the supporting granulosa cells. Morphologically, one of the earliest conserved events of dying cells is cell shrinkage and recent studies by ..
  61. Mechanisms of luteinization in the primate ovary
    CHARLES CHAFFIN; Fiscal Year: 2007
    Recent evidence in primates demonstrates that proliferation of granulosa cells is arrested within the first quarter of the periovulatory interval. However, specific markers of cell cycle progression, e.g...
  62. Cellular and Molecular Mechanisms of Mammalian Ovulation
    Ok Kyong Park Sarge; Fiscal Year: 2002
    ..the synthesis of progesterone and its intracellular receptors, progesterone receptors (PRs), in the granulosa cells of preovulatory follicles...
  63. ACTIVE PROTEIN KINASE IN VIVO AND IN VITRO
    William Fletcher; Fiscal Year: 1990
    ..protein (PKI); 2) Evaluate the quantitative relationship between the amounts of hormone bound by ovarian granulosa cells and the resultant dissociation of R and C and activity of the PKI; 3) Determine whether or not cAMP is the ..
  64. PHYSIOLOGICAL MEDIATORS OF GRANULOSA CELL APOPTOSIS
    Alan Johnson; Fiscal Year: 2000
    ..Similarly, factors that render granulosa cells resistant to apoptosis, and thus permit follicles to proceed through the process of final differentiation, ..
  65. INTRAFOLLICULAR REGULATION OF OVARIAN TGF-B1 EXPRESSION
    JEFFREY MAY; Fiscal Year: 1999
    ..and secrete TGF-beta1 during follicle development but that it is under the direction of FSH acting upon granulosa cells. We further hypothesize that a FSH-stimulated granulosa cell product regulates theca cell TGF-beta expression ..