transformed cell line

Summary

Summary: Eukaryotic cell line obtained in a quiescent or stationary phase which undergoes conversion to a state of unregulated growth in culture, resembling an in vitro tumor. It occurs spontaneously or through interaction with viruses, oncogenes, radiation, or drugs/chemicals.

Top Publications

  1. ncbi Mutations in the phosphatidylinositol-3-kinase pathway predict for antitumor activity of the inhibitor PX-866 whereas oncogenic Ras is a dominant predictor for resistance
    Nathan T Ihle
    M D Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, TX 77030, USA
    Cancer Res 69:143-50. 2009
  2. ncbi Reversal of senescence in mouse fibroblasts through lentiviral suppression of p53
    Annette M G Dirac
    Division of Molecular Carcinogenesis, and Center for Biomedical Genetics, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    J Biol Chem 278:11731-4. 2003
  3. ncbi Allosteric inhibitors of Bcr-abl-dependent cell proliferation
    Francisco J Adrian
    Biological Chemistry Department, Genomics Institute of the Novartis Research Foundation, 10675 John Jay Hopkins Drive, San Diego, California 92121, USA
    Nat Chem Biol 2:95-102. 2006
  4. ncbi Impact of the central polypurine tract on the kinetics of human immunodeficiency virus type 1 vector transduction
    Bénédicte Van Maele
    Rega Institute for Medical Research, Leuven, Flanders, Belgium
    J Virol 77:4685-94. 2003
  5. ncbi ATM and DNA-PK function redundantly to phosphorylate H2AX after exposure to ionizing radiation
    Tom Stiff
    Genome Damage and Stability Centre, University of Sussex, East Sussex, United Kingdom
    Cancer Res 64:2390-6. 2004
  6. ncbi Multiple centrosomes arise from tetraploidy checkpoint failure and mitotic centrosome clusters in p53 and RB pocket protein-compromised cells
    Franck Borel
    Institut de Biologie Structurale Jean-Pierre Ebel, , 41 rue Jules Horowitz, 38027 Grenoble Cedex 1, France
    Proc Natl Acad Sci U S A 99:9819-24. 2002
  7. ncbi Mlx, a new Max-like bHLHZip family member: the center stage of a novel transcription factors regulatory pathway?
    G Meroni
    Telethon Institute of Genetics and Medicine, Milan, Italy
    Oncogene 19:3266-77. 2000
  8. ncbi Ras transformation requires metabolic control by 6-phosphofructo-2-kinase
    S Telang
    Molecular Targets Program, James Graham Brown Cancer Center, University of Louisville, Louisville, KY 40202, USA
    Oncogene 25:7225-34. 2006
  9. ncbi Requirement of mammalian DNA polymerase-beta in base-excision repair
    R W Sobol
    Sealy Center for Molecular Science, University of Texas Medical Branch, Galveston 77555 1068, USA
    Nature 379:183-6. 1996
  10. ncbi Various members of the Toll-like receptor family contribute to the innate immune response of human epidermal keratinocytes
    Gabriele Köllisch
    Division of Environmental Dermatology and Allergy GSF TUM, GSF National Research Center for Environment and Health, Neuherberg, Germany
    Immunology 114:531-41. 2005

Research Grants

  1. NQO1 Inhibitors and Pancreatic Cancer Therapy
    David Ross; Fiscal Year: 2007
  2. HMG-CoA reductase inhibitor, tea polyphenols and pancreatic cancer prevention
    Guang Yu Yang; Fiscal Year: 2007
  3. TUBULOINTERSTITIAL KIDNEY CELLS & REPONSE TO GLUCOSE
    Fuad Ziyadeh; Fiscal Year: 1993
  4. HIV1 INHIBITORY PROTEINS FROM TRANSFORMED CD8+ CELLS
    Mary Klotman; Fiscal Year: 2002
  5. ION CHANNELS AND MONONUCLEAR PHAGOCYTE ACTIVATION
    Deborah Nelson; Fiscal Year: 2001
  6. Anti-HPV RNA Interference Using Modified RNA's
    Kenneth Alexander; Fiscal Year: 2005
  7. MECHANISM OF MOUSE LUNG CARCINOGENESIS
    Ming You; Fiscal Year: 2006
  8. BIOCHEMISTRY OF ANIMAL VIRUS MULTIPLICATION
    Maurice Green; Fiscal Year: 1980
  9. Regulation of Oocyte Viability by Granulosa Cell Contact
    John Peluso; Fiscal Year: 2004
  10. PAIRBP & PGRMC1 act as a membrane receptor complex to mediate P4's ovarian action
    John J Peluso; Fiscal Year: 2010

Detail Information

Publications189 found, 100 shown here

  1. ncbi Mutations in the phosphatidylinositol-3-kinase pathway predict for antitumor activity of the inhibitor PX-866 whereas oncogenic Ras is a dominant predictor for resistance
    Nathan T Ihle
    M D Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, TX 77030, USA
    Cancer Res 69:143-50. 2009
    ....
  2. ncbi Reversal of senescence in mouse fibroblasts through lentiviral suppression of p53
    Annette M G Dirac
    Division of Molecular Carcinogenesis, and Center for Biomedical Genetics, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    J Biol Chem 278:11731-4. 2003
    ..These data indicate that senescence in MEFs is reversible and demonstrate that both initiation and maintenance of senescence is p53-dependent...
  3. ncbi Allosteric inhibitors of Bcr-abl-dependent cell proliferation
    Francisco J Adrian
    Biological Chemistry Department, Genomics Institute of the Novartis Research Foundation, 10675 John Jay Hopkins Drive, San Diego, California 92121, USA
    Nat Chem Biol 2:95-102. 2006
    ..We propose that this new class of compounds inhibits Bcr-abl kinase activity through an allosteric non-ATP competitive mechanism...
  4. ncbi Impact of the central polypurine tract on the kinetics of human immunodeficiency virus type 1 vector transduction
    Bénédicte Van Maele
    Rega Institute for Medical Research, Leuven, Flanders, Belgium
    J Virol 77:4685-94. 2003
    ....
  5. ncbi ATM and DNA-PK function redundantly to phosphorylate H2AX after exposure to ionizing radiation
    Tom Stiff
    Genome Damage and Stability Centre, University of Sussex, East Sussex, United Kingdom
    Cancer Res 64:2390-6. 2004
    ..However, by phosphorylating H2AX, DNA-PK can contribute to the presence of the damage response proteins MDC1 and 53BP1 at the site of the DSB...
  6. ncbi Multiple centrosomes arise from tetraploidy checkpoint failure and mitotic centrosome clusters in p53 and RB pocket protein-compromised cells
    Franck Borel
    Institut de Biologie Structurale Jean-Pierre Ebel, , 41 rue Jules Horowitz, 38027 Grenoble Cedex 1, France
    Proc Natl Acad Sci U S A 99:9819-24. 2002
    ..Finally, we find that mouse embryo fibroblasts spontaneously enter tetraploid G(1), explaining the previous demonstration of centrosome amplification by p53 abrogation alone in these cells...
  7. ncbi Mlx, a new Max-like bHLHZip family member: the center stage of a novel transcription factors regulatory pathway?
    G Meroni
    Telethon Institute of Genetics and Medicine, Milan, Italy
    Oncogene 19:3266-77. 2000
    ..The possible role of Mlx in an emerging regulatory pathway and acting parallel to the Max driven network is discussed...
  8. ncbi Ras transformation requires metabolic control by 6-phosphofructo-2-kinase
    S Telang
    Molecular Targets Program, James Graham Brown Cancer Center, University of Louisville, Louisville, KY 40202, USA
    Oncogene 25:7225-34. 2006
    ....
  9. ncbi Requirement of mammalian DNA polymerase-beta in base-excision repair
    R W Sobol
    Sealy Center for Molecular Science, University of Texas Medical Branch, Galveston 77555 1068, USA
    Nature 379:183-6. 1996
    ..These studies demonstrate that beta-polymerase functions specifically in base-excision repair in vivo...
  10. ncbi Various members of the Toll-like receptor family contribute to the innate immune response of human epidermal keratinocytes
    Gabriele Köllisch
    Division of Environmental Dermatology and Allergy GSF TUM, GSF National Research Center for Environment and Health, Neuherberg, Germany
    Immunology 114:531-41. 2005
    ..These studies provide substantial evidence for a functional TLR expression and signalling profile of normal human keratinocytes contributing to the antimicrobial defence barrier of human skin...
  11. ncbi Human podocytes possess a stretch-sensitive, Ca2+-activated K+ channel: potential implications for the control of glomerular filtration
    Michael J Morton
    School of Biomedical Sciences, Worsley Building, University of Leeds, Leeds LS2 9NQ, UK
    J Am Soc Nephrol 15:2981-7. 2004
    ..By implication, these results suggest that podocytes may be able to respond to changes in the glomerular capillary pressure and modulate the GFR...
  12. ncbi Dynamics of telomere erosion in transformed and non-transformed avian cells in vitro
    S E Swanberg
    Department of Animal Science, University of California, Davis, CA 95616, USA
    Cytogenet Genome Res 102:318-25. 2003
    ..Further, monitoring of telomere lengths of primary cells provides evidence for a dynamic series of changes over the lifespan of any specific cell culture ultimately resulting in net telomeric DNA loss by senescence...
  13. ncbi Genome-wide SNP assay reveals structural genomic variation, extended homozygosity and cell-line induced alterations in normal individuals
    Javier Simon Sanchez
    Molecular Genetics Unit, Departamento de Genómica y Proteómica, Instituto de Biomedicina de Valencia CSIC, 46010, Valencia, Spain
    Hum Mol Genet 16:1-14. 2007
    ..It is likely that this powerful methodology will augment existing techniques in the identification of chromosomal abnormalities...
  14. ncbi Reconstitution of telomerase activity combined with HPV-E7 expression allow human preadipocytes to preserve their differentiation capacity after immortalization
    C Darimont
    Nestle Research Center, PO Box 44, Vers chez les Blanc, 1000 Lausanne 26, Switzerland
    Cell Death Differ 10:1025-31. 2003
    ..These data demonstrate, for the first time, that coexpression of hTERT and HPV-E7 in human preadipocytes allows cells not only to display an indefinite life span but also to retain their capacity to differentiate...
  15. ncbi Site-directed mutagenesis of the ATM promoter: consequences for response to proliferation and ionizing radiation
    Nuri Gueven
    Queensland Cancer Fund Research Laboratory, The Queensland Institute of Medical Research, Royal Brisbane Hospital, Brisbane, Queensland, Australia
    Genes Chromosomes Cancer 38:157-67. 2003
    ..The data described here provide additional evidence that basal and radiation-induced regulation of the ATM promoter is under multifactorial control...
  16. ncbi Poly(ADP-ribosyl)ation accelerates DNA repair in a pathway dependent on Cockayne syndrome B protein
    Claudia Flohr
    Institute of Pharmacy, University of Mainz, 55099 Mainz, Germany
    Nucleic Acids Res 31:5332-7. 2003
    ..The results indicate that PARP activation and Csb are both involved in a novel mechanism that accelerates the global repair of various types of DNA modifications...
  17. ncbi Association of gp91phox homolog Nox1 with anchorage-independent growth and MAP kinase-activation of transformed human keratinocytes
    Walee Chamulitrat
    Deutsches Krebsforschungszentrum, Department of Applied Tumorvirology, 69120 Heidelberg, Germany
    Oncogene 22:6045-53. 2003
    ..Furthermore, JNK2 was constitutively phosphorylated in FIB cells. Together, our data strongly implicate Nox1 in redox-mediated signaling related to cellular activation of human keratinocytes at a more advanced stage of transformation...
  18. ncbi The second loop of occludin is required for suppression of Raf1-induced tumor growth
    Zili Wang
    Epithelial Pathobiology Research Unit, Department of Pathology, Emory University, 615 Michael Street, Atlanta, GA 30322, USA
    Oncogene 24:4412-20. 2005
    ..These results demonstrate that the TJ protein occludin has a potent inhibitory effect on the Raf1-mediated tumorigenesis, and the second extracellular loop of occludin appears to be critical for this function...
  19. ncbi Developmentally regulated recruitment of transcription factors and chromatin modification activities to chicken lysozyme cis-regulatory elements in vivo
    Pascal Lefevre
    Molecular Medicine Unit, University of Leeds, St James's University Hospital, Leeds LS9 7TF, United Kingdom
    Mol Cell Biol 23:4386-400. 2003
    ..This indicates that a partially accessible chromatin structure of lineage-specific genes is a hallmark of hematopoietic progenitor cells...
  20. ncbi Conditionally immortalized human glomerular endothelial cells expressing fenestrations in response to VEGF
    S C Satchell
    Academic Renal Unit, University of Bristol, Southmead Hospital, Bristol, UK
    Kidney Int 69:1633-40. 2006
    ..They express fenestrations which are upregulated in response to VEGF. These cells are a unique resource for further study of GEnC and their roles in glomerular filtration, glomerular disease, and response to glomerular injury...
  21. ncbi Brain transplantation of immortalized human neural stem cells promotes functional recovery in mouse intracerebral hemorrhage stroke model
    Hong J Lee
    Division of Neurology, Department of Medicine, University of British Columbia Hospital, University of British Columbia, Vancouver, British Columbia V6T 2B5, Canada
    Stem Cells 25:1204-12. 2007
    ..Disclosure of potential conflicts of interest is found at the end of this article...
  22. ncbi Mechanical strain increases SPARC levels in podocytes: implications for glomerulosclerosis
    Raghu V Durvasula
    Division of Nephrology, Box 356521, Univ of Washington School of Medicine, Seattle, WA 98195, USA
    Am J Physiol Renal Physiol 289:F577-84. 2005
    ..We speculate that the increase in SPARC may be maladaptive and lead to a progressive reduction in podocyte number, thus fueling the future development of glomerulosclerosis...
  23. ncbi Iron and contact with host cells induce expression of adhesins on surface of Trichomonas vaginalis
    Ana F Garcia
    Department of Microbiology, University of Texas Health Science Center, San Antonio, TX, USA
    Mol Microbiol 47:1207-24. 2003
    ..Overall, the results confirm an important role for iron and contact in the surface expression of adhesins of T. vaginalis organisms...
  24. ncbi Genotoxic stress and cellular stress alter the subcellular distribution of human T-cell leukemia virus type 1 tax through a CRM1-dependent mechanism
    Michael L Gatza
    Department of Molecular Virology and Microbiology, Baylor College of Medicine, One Baylor Plaza MS-385, Houston, TX 77030, USA
    J Virol 80:6657-68. 2006
    ..Changes in Tax distribution and interacting partners are likely to affect cellular processes that regulate cellular transformation...
  25. ncbi Evidence that the retroviral DNA integration process triggers an ATR-dependent DNA damage response
    Rene Daniel
    Institute for Cancer Research, Fox Chase Cancer Center, 7701 Burholme Avenue, Philadelphia, PA 19111, USA
    Proc Natl Acad Sci U S A 100:4778-83. 2003
    ..Components of the cellular DNA damage repair response may represent potential targets for antiretroviral drug development...
  26. ncbi Activation of the ATR-mediated DNA damage response by the HIV-1 viral protein R
    Mikhail Roshal
    Department of Microbiology and Immunology, University of Rochester Cancer Center, Rochester, New York 14642, USA
    J Biol Chem 278:25879-86. 2003
    ..These observations are important toward understanding how HIV infection promotes cell cycle disruption, cell death, and ultimately, CD4+ lymphocyte depletion...
  27. ncbi Construction of a full-length human T cell leukemia virus type I genome from MT-2 cells containing multiple defective proviruses using overlapping polymerase chain reaction
    Takeo Ohsugi
    Division of Microbiology and Genetics, Center for Animal Resources and Development, Institute of Resource Development and Analysis, Kumamoto University, 2 2 1 Honjo, Kumamoto 860 0811, Japan
    Anal Biochem 329:281-8. 2004
    ..polymerase chain reaction (PCR) to construct a full-length provirus of HTLV-I directly from an HTLV-I-transformed cell line, MT-2, which contains multiple defective proviruses. First, four overlapping proviral HTLV-I fragments (1...
  28. ncbi Titanium dioxide (P25) produces reactive oxygen species in immortalized brain microglia (BV2): implications for nanoparticle neurotoxicity
    Thomas C Long
    Department of Environmental Sciences and Engineering, School of Public Health, University of North Carolina, Chapel Hill, North Carolina 27599-7431, USA
    Environ Sci Technol 40:4346-52. 2006
    ..Cell viability was maintained at all test concentrations (2.5-120 ppm) over the 18 h exposure period. These data indicate that mouse microglia respond to Degussa P25 with cellular and morphological expressions of free radical formation...
  29. ncbi EBNA-1, a bifunctional transcriptional activator
    Gregory Kennedy
    McArdle Laboratory for Cancer Research, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA
    Mol Cell Biol 23:6901-8. 2003
    ..EBNA-1's mode of activating transfected templates is therefore genetically distinct from that acting on integrated templates...
  30. ncbi Matricellular protein SPARC is translocated to the nuclei of immortalized murine lens epithelial cells
    Qi Yan
    Hope Heart Program, Benaroya Research Institute at Virginia Mason, Seattle, Washington 98104 2046, USA
    J Cell Physiol 203:286-94. 2005
    ..SPARC therefore functions not only as an extracellular protein but also potentially as an intracellular protein to influence cellular activities and homeostasis...
  31. ncbi Serum deprivation induces apoptotic cell death of transformed rat retinal ganglion cells via mitochondrial signaling pathways
    Irma Charles
    Department of Cell Biology and Genetics, University of North Texas Health Science Center, Fort Worth, Texas, USA
    Invest Ophthalmol Vis Sci 46:1330-8. 2005
    ..These studies lead to the speculation that growth factor deprivation arising from blockade of retrograde transport of neurotrophins may involve similar mechanism(s) of retinal ganglion cell death in glaucoma...
  32. ncbi Human neural stem cells genetically modified for brain repair in neurological disorders
    Seung U Kim
    Brain Disease Research Center, Ajou University School of Medicine, Suwon, Korea
    Neuropathology 24:159-71. 2004
    ..There is further need for investigation into the neurogenic potential of the human bone marrow stem cell lines and their utility in animal models of neurological diseases...
  33. ncbi Specific packaging of APOBEC3G into HIV-1 virions is mediated by the nucleocapsid domain of the gag polyprotein precursor
    Alexandra Schäfer
    Howard Hughes Medical Institute and Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC 27710, USA
    Virology 328:163-8. 2004
    ..Surprisingly, RNA was also found to be essential for formation of the nucleocapsid--APOBEC3G complex in vitro, thus raising the possibility that RNA may form a bridge between these two proteins...
  34. ncbi Establishment of immortal swine kidney epithelial cells
    Sungwook Kwak
    Laboratory of Cell Growth and Function Regulation, Division of Bioscience and Technology, College of Life and Environmental Sciences, Korea University, Seoul, Korea
    Anim Biotechnol 17:51-8. 2006
    ..Hence, three immortalized swine kidney epithelial cells that are not transformed would be valuable biological tools for virus propagation and basic kidney epithelial cell research...
  35. ncbi Spontaneously immortalized human T lymphocytes develop gain of chromosomal region 2p13-24 as an early and common genetic event
    Jan Konrad Siwicki
    Department of Immunology, Maria Skłodowska Curie Memorial Cancer Center and Institute of Oncology, Warsaw, Poland
    Genes Chromosomes Cancer 41:133-44. 2004
    ....
  36. ncbi Proactive transplantation of human neural stem cells prevents degeneration of striatal neurons in a rat model of Huntington disease
    Jae K Ryu
    Brain Disease Research Center, Ajou University School of Medicine, Suwon, South Korea
    Neurobiol Dis 16:68-77. 2004
    ..The findings in this work have particular relevance to a rat model of HD in that proactive transplanted hNSCs protect host striatal neurons against neuronal injury and improve motor impairment induced by 3-NP toxicity...
  37. ncbi v-Myb mediates cooperation of a cell-specific enhancer with the mim-1 promoter
    Olesya Chayka
    Institut fur Biochemie, Westfalische Wilhelms Universitat Munster, Wilhelm Klemm Strasse 2, D 48149 Munster, Germany
    Mol Cell Biol 25:499-511. 2005
    ....
  38. ncbi Spatio-temporal organization of DNA replication in murine embryonic stem, primary, and immortalized cells
    Margaret M Panning
    Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, 750 East Adams Street, Syracuse, New York 13210, USA
    J Cell Biochem 95:74-82. 2005
    ..These data suggest that heritable gene silencing during commitment to specific cell lineages is not mediated by global changes in the sub-nuclear organization and replication timing of chromosome domains...
  39. ncbi The 33 kb transcript of the chicken alpha-globin gene domain is part of the nuclear matrix
    Sergey V Razin
    Institut J Monod, 2, place Jussieu, 75251 Paris, Cedex 05, France
    J Cell Biochem 92:445-57. 2004
    ..They may take part in the dynamic nuclear architecture when productively processed, or turn over slowly when globins are not synthesised...
  40. ncbi Telomere maintenance requires the RAD51D recombination/repair protein
    Madalena Tarsounas
    Cancer Research UK, London Research Institute, Clare Hall Laboratories, Blanche Lane, South Mimms, Hertfordshire, EN6 3LD, United Kingdom
    Cell 117:337-47. 2004
    ..We conclude that RAD51D plays a dual cellular role in both the repair of DNA double-strand breaks and telomere protection against attrition and fusion...
  41. ncbi Deficiency of polycystin-2 reduces Ca2+ channel activity and cell proliferation in ADPKD lymphoblastoid cells
    Gianluca Aguiari
    Department of Biochemistry and Molecular Biology, University of Ferrara, Ferrara, Italy
    FASEB J 18:884-6. 2004
    ..Therefore, PC2 and PC1 are functionally expressed in LCLs, which provide a model, easily obtainable from ADPKD patients, to study PKD gene expression and function...
  42. ncbi Lytic induction therapy for Epstein-Barr virus-positive B-cell lymphomas
    Wen-hai Feng
    Department of Medicine, and Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    J Virol 78:1893-902. 2004
    ..These results suggest that the addition of GCV to either gemcitabine- or doxorubicin-containing chemotherapy regimens may enhance the therapeutic efficacy of these drugs for EBV-driven lymphoproliferative disease in patients...
  43. ncbi Activation of a local tissue angiotensin system in podocytes by mechanical strain
    Raghu V Durvasula
    Department of Medicine, Division of Nephrology, University of Washington School of Medicine, Seattle, Washington 98195, USA
    Kidney Int 65:30-9. 2004
    ..The resulting activation of a local tissue angiotensin system leads to an increase in podocyte apoptosis, mainly in an AT1R-mediated fashion...
  44. ncbi The vimentin cytoskeleton regulates focal contact size and adhesion of endothelial cells subjected to shear stress
    Daisuke Tsuruta
    Department of Cell and Molecular Biology, Northwestern University Medical School, Morton 4-616, 303 E. Chicago Avenue, Chicago, IL 60611, USA
    J Cell Sci 116:4977-84. 2003
    ..More dramatically, such cells showed decreased adhesion to the substratum. These data provide evidence that the vimentin cytoskeleton regulates focal contact size and helps stabilize cell-matrix adhesions in endothelial cells...
  45. ncbi The mechanisms controlling the recognition of tumor- and virus-infected cells by NKp46
    Tal I Arnon
    Lautenberg Center for General and Tumor Immunology, The Hebrew University Hadassah Medical School, Jerusalem, 91120, Israel
    Blood 103:664-72. 2004
    ..These results may explain the ability of NK cells to kill such a broad spectrum of viral-infected and tumor cells...
  46. ncbi Microarray reveals complement components are regulated in the serum-deprived rat retinal ganglion cell line
    Abdelnaby Khalyfa
    Department of Anatomical Sciences and Neurobiology, University of Louisville, School of Medicine, Louisville, KY 40292, USA
    Mol Vis 13:293-308. 2007
    ..The aim of this study was to generate a genome-wide gene expression of RGC-5 following serum deprivation and to identify candidate genes that may be involved in the signal transduction pathways...
  47. ncbi Inhibition of plasminogen activators attenuates the death of differentiated retinal ganglion cells and stabilizes their neurite network in vitro
    Ronée Harvey
    Eye Research Institute of Oakland University, Rochester, MI 48309, USA
    Invest Ophthalmol Vis Sci 48:1884-91. 2007
    ....
  48. ncbi Mechanisms regulating plasminogen activators in transformed retinal ganglion cells
    Nathan Rock
    Eye Research Institute, Oakland University, Rochester, MI 48309, USA
    Exp Eye Res 86:492-9. 2008
    ..These results indicate that LRP-1 regulates autocrine synthesis of tPA and uPA in RGC-5 cells and suggest that the use of RAP to antagonize the effect of proteases may be a way to prevent RGC death in glaucoma...
  49. ncbi Measurements of the BKCa channel's high-affinity Ca2+ binding constants: effects of membrane voltage
    Tara Beth Sweet
    Molecular Cardiology Research Institute, Tufts Medical Center, Tufts University School of Medicine, Boston, MA 02111, USA
    J Gen Physiol 132:491-505. 2008
    ....
  50. ncbi DDB accumulates at DNA damage sites immediately after UV irradiation and directly stimulates nucleotide excision repair
    Mitsuo Wakasugi
    Faculty of Pharmaceutical Sciences, Kanazawa University, Takara-machi, Kanazawa 920-0934, Japan
    J Biol Chem 277:1637-40. 2002
    ..These results suggest that DDB can rapidly translocate to the damaged DNA sites independent of functional XPA and XPC proteins and directly enhance the excision reaction by core repair factors...
  51. ncbi Coenzyme Q10 protects retinal cells against oxidative stress in vitro and in vivo
    Yoshimi Nakajima
    Department of Biofunctional Evaluation, Molecular Pharmacology, Gifu Pharmaceutical University, 5 6 1 Mitahora Higashi, Gifu 502 8585, Japan
    Brain Res 1226:226-33. 2008
    ..To investigate the neuroprotective effects of coenzyme Q10 and/or a vitamin E analogue on retinal damage both in vitro and in vivo...
  52. ncbi Involvement of ER stress in retinal cell death
    Masamitsu Shimazawa
    Department of Biofunctional Molecules, Gifu Pharmaceutical University, Gifu, Japan
    Mol Vis 13:578-87. 2007
    ....
  53. ncbi Oxidative stress is an early event in hydrostatic pressure induced retinal ganglion cell damage
    Quan Liu
    Hamilton Glaucoma Center and the Department of Ophthalmology, University of California San Diego, La Jolla, California 92037 0946, USA
    Invest Ophthalmol Vis Sci 48:4580-9. 2007
    ..To determine whether oxidative adduct formation or heme oxygenase-1 (HO-1) expression are altered in retinal ganglion cell (RGC) cultures exposed to elevated hydrostatic pressure and in a mouse model of glaucoma...
  54. ncbi The best disease-linked Cl- channel hBest1 regulates Ca V 1 (L-type) Ca2+ channels via src-homology-binding domains
    Kuai Yu
    Department of Cell Biology, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    J Neurosci 28:5660-70. 2008
    ..Our findings reveal a novel function of bestrophin in regulation of Ca(V) channels and suggest a possible mechanism for the role of hBest1 in macular degeneration...
  55. ncbi Human telomerase reverse transcriptase (hTERT) Q169 is essential for telomerase function in vitro and in vivo
    Haley D M Wyatt
    Southern Alberta Cancer Research Institute and Department of Biochemistry and Molecular Biology, University of Calgary, Calgary, Alberta, Canada
    PLoS ONE 4:e7176. 2009
    ..A conserved glutamine residue in the TERT N-terminus is important for anchor site interactions in lower eukaryotes. The significance of this residue in higher eukaryotes, however, has not been investigated...
  56. ncbi Epstein-Barr virus-encoded latent membrane protein 1 (LMP1) induces the expression of the cellular microRNA miR-146a
    Natalie Motsch
    Institute of Virology, University of Saarland Medical School, Homburg, Germany
    RNA Biol 4:131-7. 2007
    ..It is possible that the induction of miR-146a plays a role in the induction or maintenance of EBV latency by modulating innate immune responses to the virus infected host cell...
  57. ncbi Replication protein A prevents accumulation of single-stranded telomeric DNA in cells that use alternative lengthening of telomeres
    Amra Grudic
    Section of Oncology, Department of Medicine, Haukeland University Hospital
    Nucleic Acids Res 35:7267-78. 2007
    ..This study demonstrates differences between ALT cells and telomerase-positive cells in the requirement for RPA in telomere processing and implicates the ALT mechanism in tumor cells as a possible therapeutic target...
  58. ncbi The oncoprotein H-RasV12 increases mitochondrial metabolism
    Sucheta Telang
    Molecular Targets Group, Department of Medicine, James Graham Brown Cancer Center, University of Louisville, Louisville, Kentucky 40202, USA
    Mol Cancer 6:77. 2007
    ....
  59. ncbi Quantitative and functional analyses of spastin in the nervous system: implications for hereditary spastic paraplegia
    Joanna M Solowska
    Department of Neurobiology and Anatomy, Drexel University College of Medicine, Philadelphia, Pennsylvania 19129, USA
    J Neurosci 28:2147-57. 2008
    ..These results are consistent with a "gain-of-function" mechanism underlying HSP wherein spastin mutations produce a cytotoxic protein in the case of M1 but not M85...
  60. ncbi Competitive ELISA using a rabies glycoprotein-transformed cell line to semi-quantify rabies neutralizing-related antibodies in dogs
    Shoufeng Zhang
    Laboratory of Epidemiology, Veterinary Institute, Academy of Military Medical Sciences, 1068 Qinglong Road, Changchun 130062, PR China
    Vaccine 27:2108-13. 2009
    ..00 IU/ml. Since it can assay 40 or more samples in 1.5h, the c-ELISA is suitable for the surveillance of mass vaccination in dogs where rabies causes public concern...
  61. ncbi MicroRNA-513 regulates B7-H1 translation and is involved in IFN-gamma-induced B7-H1 expression in cholangiocytes
    Ai Yu Gong
    Department of Medical Microbiology and Immunology, Creighton University Medical Center, Omaha, NE 68178, USA
    J Immunol 182:1325-33. 2009
    ..Thus, miR-513 regulates B7-H1 translation and is involved in IFN-gamma-induced B7-H1 expression in human cholangiocytes, suggesting a role for miRNA-mediated gene silencing in the regulation of cholangiocyte response to IFN-gamma...
  62. ncbi Genomic aberrations and cellular heterogeneity in SV40-immortalized human corneal epithelial cells
    Kenta Yamasaki
    Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kawaramachi dori, Kamigyo ku, Kyoto, Japan
    Invest Ophthalmol Vis Sci 50:604-13. 2009
    ..The nature of this cell line was assessed for genomic aberrations and cellular heterogeneity...
  63. ncbi NF-kappaB is essential for epithelial-mesenchymal transition and metastasis in a model of breast cancer progression
    Margit A Huber
    Department of Physiological Chemistry, Ulm University, Albert-Einstein-Allee 11, D-89081 Ulm, Germany
    J Clin Invest 114:569-81. 2004
    ....
  64. ncbi Identification of a serum-inducible messenger RNA (5B10) as the mouse homologue of calcyclin: tissue distribution and expression in metastatic, ras-transformed NIH 3T3 cells
    X J Guo
    Cancer Research Laboratory, University of Western Ontario, London, Canada
    Cell Growth Differ 1:333-8. 1990
    ..In addition, high 5B10 mRNA levels were associated with metastatic ability in a series of ras-transformed cells, in proportion to levels of ras p21 expressed by the cells, implicating 5B10 even more deeply in carcinogenesis...
  65. ncbi Absence of p53 complements defects in Abelson murine leukemia virus signaling
    Indira Unnikrishnan
    Department of Pathology, Tufts University School of Medicine, Boston, Massachusetts 02111, USA
    J Virol 77:6208-15. 2003
    ..These results indicate that one important transforming function of v-Abl proteins is overcoming the effects of a functional p53 pathway...
  66. ncbi Evaluation of potential mechanisms underlying genotype-phenotype correlations in multiple endocrine neoplasia type 2
    N Mise
    Department of Vectorology and Experimental Gene Therapy, University of Rostock, Rostock, Germany
    Oncogene 25:6637-47. 2006
    ..Whereas MEN 2A mutants resulted in accelerated cell proliferation, MEN 2B-RET mutants significantly enhanced suppression of apoptosis, which may account, at least partially, for some of the clinical differences in MEN 2 patients...
  67. ncbi Nectin-like molecule-5/Tage4 enhances cell migration in an integrin-dependent, Nectin-3-independent manner
    Wataru Ikeda
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Suita 565 0871, Osaka, Japan
    J Biol Chem 279:18015-25. 2004
    ..We furthermore show here that enhanced motility and metastasis of V12Ras-NIH3T3 cells are at least partly the result of up-regulated Necl-5...
  68. ncbi Cdc42 and Ras cooperate to mediate cellular transformation by intersectin-L
    Jian Bin Wang
    Department of Molecular Medicine, Veterinary Medical Center, Cornell University, Ithaca, New York 14853, USA
    J Biol Chem 280:22883-91. 2005
    ..Rather, we found that SH3A-C2 exhibited strong transforming activity through its ability to mediate cooperation between Ras and Cdc42...
  69. ncbi Identification of potent Ras signaling inhibitors by pathway-selective phenotype-based screening
    Oliver Muller
    Max Planck Institut fur molekulare Physiologie, Abteilung Strukturelle Biologie, Dortmund, Germany
    Angew Chem Int Ed Engl 43:450-4. 2004
  70. ncbi In vitro models of early neoplastic transformation of human mammary epithelial cells
    Vimla Band
    Department of Radiation Oncology, New England Medical Center, Boston, MA, USA
    Methods Mol Biol 223:237-48. 2003
  71. ncbi Purified scatter factor stimulates epithelial and vascular endothelial cell migration
    E M Rosen
    Department of Therapeutic Radiology, Yale University School of Medicine, New Haven, Connecticut 06510
    Proc Soc Exp Biol Med 195:34-43. 1990
    ..ras-3T3 SF appears to be a new protein which regulates endothelial and epithelial mobility; and, therefore, it may be involved in vascular repair and wound healing...
  72. ncbi Gene expression profiling of tumours derived from rasV12/E1A-transformed mouse embryonic fibroblasts to identify genes required for tumour development
    Sophie Vasseur
    INSERM U, 624, Stress Cellulaire, 163 Avenue de Luminy, Case 915, Parc Scientifique et Technologique de Luminy, 13288 Marseille Cedex 9, France
    Mol Cancer 4:4. 2005
    ....
  73. ncbi IL-8-mediated cell migration in endothelial cells depends on cathepsin B activity and transactivation of the epidermal growth factor receptor
    Ingrid U Schraufstatter
    Department of Cancer Biology, La Jolla Institute for Molecular Medicine, San Diego, CA 92121, USA
    J Immunol 171:6714-22. 2003
    ..These results indicate that IL-8-induced transactivation of the EGFR is mediated by the CXCR2 and involves cathepsin B, and that this pathway is important for the migratory and tumorigenic effects of IL-8...
  74. ncbi Ability of the activated PI3K/Akt oncoproteins to synergize with MEK1 and induce cell cycle progression and abrogate the cytokine-dependence of hematopoietic cells
    John G Shelton
    Department of Microbiology and Immunology, Brody School of Medicine, East Carolina University, Greenville, North Carolina 27858, USA
    Cell Cycle 3:503-12. 2004
    ..Likewise, suppression of multiple signal transduction pathways is a more effective means to inhibit cell cycle progression and induce apoptosis in leukemic cells...
  75. ncbi Combined loss of INK4a and caveolin-1 synergistically enhances cell proliferation and oncogene-induced tumorigenesis: role of INK4a/CAV-1 in mammary epithelial cell hyperplasia
    Terence M Williams
    Department of Molecular Pharmacology, Albert Einsten Cancer Center, Albert Einsten College of Medicine, Bronx, New York 10461, USA
    J Biol Chem 279:24745-56. 2004
    ..Our results provide important new evidence for the transformation suppressor properties of Cav-1 and the first molecular genetic evidence that Cav-1 cooperates with a tumor suppressor, namely the INK4a genetic locus...
  76. ncbi Phospholipase D activity is elevated in hepatitis C virus core protein-transformed NIH3T3 mouse fibroblast cells
    Joonmo Kim
    Department of Physiology, College of Medicine, The Catholic University of Korea, Seoul 137-701, Korea
    Exp Mol Med 36:454-60. 2004
    ..Taken together, these results suggest that PLD might be implicated in core protein-induced transformation...
  77. ncbi Endoglin promotes endothelial cell proliferation and TGF-beta/ALK1 signal transduction
    Franck Lebrin
    Division of Cellular Biochemistry, The Netherlands Cancer Institute, Plesmanlaan, Amsterdam, The Netherlands
    EMBO J 23:4018-28. 2004
    ..Our results indicate a pivotal role for endoglin in the balance of ALK1 and ALK5 signalling to regulate endothelial cell proliferation...
  78. ncbi VEGF-null cells require PDGFR alpha signaling-mediated stromal fibroblast recruitment for tumorigenesis
    Jianying Dong
    Department of Molecular Oncology, Genentech Inc, South San Francisco, CA 94080, USA
    EMBO J 23:2800-10. 2004
    ..Thus, PDGFR alpha signaling is required for the recruitment of VEGF-producing stromal fibroblasts for tumor angiogenesis and growth. Our findings highlight a novel aspect of PDGFR alpha signaling in tumorigenesis...
  79. ncbi Stochastic cancer progression driven by non-clonal chromosome aberrations
    Henry H Q Heng
    Center for Molecular Medicine and Genetics, Wayne State University School of Medicine, Detroit, MI 48201, USA
    J Cell Physiol 208:461-72. 2006
    ..The dynamic relationship between NCCAs and CCAs provides a mechanism underlying chromosomal based cancer evolution and could have broad clinical applications...
  80. ncbi Differential effects of X-ALK fusion proteins on proliferation, transformation, and invasion properties of NIH3T3 cells
    Florence Armstrong
    Inserm U.563, Centre de Physiopathologie de Toulouse-Purpan, Department of 'Oncogenesis and Signaling in Hematopoietic cells, Toulouse, France
    Oncogene 23:6071-82. 2004
    ..These findings may have clinical implications in the pathogenesis and prognosis of ALK-positive ALCLs...
  81. ncbi Morphological and transcriptional responses of untransformed intestinal epithelial cells to an oncogenic beta-catenin protein
    Yasuyoshi Naishiro
    Chemotherapy Division and Cancer Proteomics Project, National Cancer Center Research Institute, 5 1 1 Tsukiji, Chuo Ku, Tokyo 104 0045, Japan
    Oncogene 24:3141-53. 2005
    ..The IGFBP2 protein interacts with integrins. Disruption of the multigene network system regulating cell adhesion and cytoskeleton may be crucial in the initiation of colorectal carcinogenesis...
  82. ncbi Evidence that mitogen-activated protein kinase phosphatase-1 induction by proteasome inhibitors plays an antiapoptotic role
    George W Small
    The Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 7295, USA
    Mol Pharmacol 66:1478-90. 2004
    ..Furthermore, they suggest that a proteasome inhibitor/anthracycline regimen holds potential for enhanced antitumor activity in part through repression of MKP-1, supporting clinical evaluation of such combinations...
  83. ncbi Exploration of the genes responsible for unlimited proliferation of immortalized lung fibroblasts
    Keiko Hiyama
    Department of Translational Cancer Research, Research Institute for Radiation Biology and Medicine, Hiroshima University, Hiroshima, Japan
    Exp Lung Res 34:373-90. 2008
    ....
  84. ncbi The Rac exchange factor Tiam1 is required for the establishment and maintenance of cadherin-based adhesions
    Angeliki Malliri
    Netherlands Cancer Institute, Division of Cell Biology, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    J Biol Chem 279:30092-8. 2004
    ..These studies indicate that the Rac activator Tiam1 is essential for the formation as well as the maintenance of cadherin-based adhesions...
  85. ncbi A methylation profile of in vitro immortalized human cell lines
    Limin Liu
    Division of Biology, Beckman Research Institute of the City of Hope, Duarte, CA 91010, USA
    Int J Oncol 26:275-85. 2005
    ..In general, more genes were methylated in neoplastically-transformed cell lines than in only immortalized cell lines, indicating that accumulation of epigenetic abnormalities may contribute to oncogenesis...
  86. ncbi Decreased tumorigenicity of c-Myc-transformed fibroblasts expressing active USF2
    Chungyoul Choe
    Department of Molecular Genetics, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA
    Exp Cell Res 302:1-10. 2005
    ..Cotransfection assays with USF- or Myc-specific dominant-negative mutants indicated that active USF2 inhibited cellular transformation by preventing transcriptional repression by c-Myc...
  87. ncbi The HPV-16 E7 oncogene sensitizes malignant cells to IFN-alpha-induced apoptosis
    Lena Thyrell
    Department of Oncology and Pathology, Cancer Center Karolinska (CCK, Karolinska Hospital and Institute, S-171 76 Stockholm, Sweden
    J Interferon Cytokine Res 25:63-72. 2005
    ..This demonstrates that an oncogenic event may alter the cellular sensitivity to IFN and might also have implications for treatment of HPV-related diseases with IFN...
  88. ncbi Interferon consensus sequence binding protein (ICSBP; IRF-8) antagonizes BCR/ABL and down-regulates bcl-2
    Andreas Burchert
    Klinikum der Philipps Universität Marburg, Klinik für Hämatologie, Onkologie und Immunologie, Marburg, Germany
    Blood 103:3480-9. 2004
    ....
  89. ncbi Defective p53 post-translational modification required for wild type p53 inactivation in malignant epithelial cells with mdm2 gene amplification
    Chad D Knights
    Department of Dermatology, Oregon Health and Science University, Portland, Oregon 97239, USA
    J Biol Chem 278:52890-900. 2003
    ..These findings suggest therapeutic strategies that address both p53/Mdm2 interaction and associated p53 protein defects in human tumors that have amplified mdm2 genes...
  90. ncbi Adhesion to fibronectin selectively protects Bcr-Abl+ cells from DNA damage-induced apoptosis
    H van der Kuip
    Dr Margarete Fischer-Bosch Institute for Clinical Pharmacology, Stuttgart, Germany
    Blood 98:1532-41. 2001
    ..Thus, cooperative activation of PI-3K/AKT by Bcr-Abl and integrins causes synergistic protection of Bcr-Abl+ cells from DNA damage-induced apoptosis...
  91. ncbi The deubiquitinating enzyme DUB-2 prolongs cytokine-induced signal transducers and activators of transcription activation and suppresses apoptosis following cytokine withdrawal
    T S Migone
    DNAX Research Institute of Molecular and Cellular Biology, Palo Alto, CA, USA
    Blood 98:1935-41. 2001
    ....
  92. ncbi R-Ras promotes tumor growth of cervical epithelial cells
    Héctor Rincón-Arano
    Immunology Department, Instituto de Investigaciones Biomedicas, Universidad Nacional Autonoma de Mexico, Mexico City, Mexico
    Cancer 97:575-85. 2003
    ..R-Ras also reportedly induces a more invasive phenotype in breast epithelial cells through integrin activation. The authors studied the mechanisms whereby R-Ras induces a malignant phenotype...
  93. ncbi Carboxyl-terminal modulator protein (CTMP), a negative regulator of PKB/Akt and v-Akt at the plasma membrane
    S M Maira
    Friedrich Miescher Institute, Post Office Box 2543, CH 4002 Basel, Switzerland, Institute for Research in Biomedicine, CH 6500 Bellinzona, Switzerland
    Science 294:374-80. 2001
    ..These findings identify CTMP as a negative regulatory component of the pathway controlling PKB activity...
  94. ncbi Roles of JNK-1 and p38 in selective induction of apoptosis by capsaicin in ras-transformed human breast epithelial cells
    Hye Jung Kang
    College of Pharmacy, Duksung Women s University, 4129 Ssangmun dong, Tobong ku, Seoul 132 714, Korea
    Int J Cancer 103:475-82. 2003
    ....
  95. ncbi Progressive transformation of immortalized esophageal epithelial cells
    Zhong Ying Shen
    Department of Tumor Pathology, Medical College of Shantou University, Guandong Province, China
    World J Gastroenterol 8:976-81. 2002
    ....
  96. ncbi RAW 264.7 macrophages induce apoptosis selectively in transformed fibroblasts: intercellular signaling based on reactive oxygen and nitrogen species
    Stefanie Heigold
    Abteilung Virologie, , , Germany
    J Leukoc Biol 72:554-63. 2002
    ..Signaling by reactive oxygen and nitrogen species seems to represent a hitherto unrecognized signaling principle for the selective elimination of potential tumor cells by macrophages...
  97. ncbi Lytic replication-associated protein (RAP) encoded by Kaposi sarcoma-associated herpesvirus causes p21CIP-1-mediated G1 cell cycle arrest through CCAAT/enhancer-binding protein-alpha
    Frederick Y Wu
    Molecular Virology Laboratories, Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, MD 21231 1000, USA
    Proc Natl Acad Sci U S A 99:10683-8. 2002
    ..Therefore, C/EBPalpha is essential for the p21-mediated inhibition of G(1) to S-phase progression by RAP in KSHV-infected host cells...
  98. ncbi A dominant repression domain in Tbx3 mediates transcriptional repression and cell immortalization: relevance to mutations in Tbx3 that cause ulnar-mammary syndrome
    H Carlson
    Shriners Hospitals for Children and Department of Cell and Developmental Biology, Oregon Health Sciences University, 3101 Sam Jackson Park Road, Portland, OR 97201, USA
    Hum Mol Genet 10:2403-13. 2001
    ..Our results identify critical functional domains within the Tbx3 protein and facilitate interpretation of the functional consequences of present and future UMS mutations...
  99. ncbi RET/PTC1 oncogene signaling in PC Cl 3 thyroid cells requires the small GTP-binding protein Rho
    M V Barone
    Centro di Endocrinologia ed Oncologia Sperimentale del CNR, c/o Dipartimento di Biologia e Patologia Cellulare e Molecolare, , Via S. Pansini 5, Naples, Italy
    Oncogene 20:6973-82. 2001
    ..We conclude that Rho is implicated in the actin reorganization and cell survival mediated by the chimeric RET/PTC1 oncogene in thyroid epithelial cells, both phenotypes being cell type- and oncogene type-specific...
  100. ncbi In vivo effects of the Epstein-Barr virus small RNA EBER-1 on protein synthesis and cell growth regulation
    Kenneth G Laing
    Department of Biochemistry and Immunology, St. George's Hospital Medical School, Cranmer Terrace, London, United Kingdom
    Virology 297:253-69. 2002
    ....
  101. ncbi Ras-inducible immortalized fibroblasts: focus formation without cell cycle deregulation
    Kivin Jacobsen
    Department of Molecular Cell Biology, University of Copenhagen, DK 1353 Copenhagen, Denmark
    Oncogene 21:3058-67. 2002
    ....

Research Grants93

  1. NQO1 Inhibitors and Pancreatic Cancer Therapy
    David Ross; Fiscal Year: 2007
    ..This approach represents a novel therapeutic strategy for the treatment of pancreatic cancer, a disease where therapeutic options are very limited and where chemotherapy has made minimal impact. ..
  2. HMG-CoA reductase inhibitor, tea polyphenols and pancreatic cancer prevention
    Guang Yu Yang; Fiscal Year: 2007
    ..be performed using the selected immortalized human pancreatic ductal epithelial cell line and its k-ras transformed cell line in vitro...
  3. TUBULOINTERSTITIAL KIDNEY CELLS & REPONSE TO GLUCOSE
    Fuad Ziyadeh; Fiscal Year: 1993
    ..Preliminary data which we have gathered suggest that a transformed cell line derived from mouse cortical tubular epithelium represents an excellent model system for evaluating the ..
  4. HIV1 INHIBITORY PROTEINS FROM TRANSFORMED CD8+ CELLS
    Mary Klotman; Fiscal Year: 2002
    ..and potent soluble factor by completing the cloning of the single 8kd protein isolated from a CD8+ HVS- transformed cell line, expression and purification of the inhibitory protein in a bacterial expression system, determination of ..
  5. ION CHANNELS AND MONONUCLEAR PHAGOCYTE ACTIVATION
    Deborah Nelson; Fiscal Year: 2001
    ..and changes in intracellular pH in both primary human-derived macrophage cell lines and a macrophage-like transformed cell line. The goal will be to determine intervention points at which phagocytosis could be uncoupled from secretion...
  6. Anti-HPV RNA Interference Using Modified RNA's
    Kenneth Alexander; Fiscal Year: 2005
    ..Similar results have been obtained in SW756 cells (a second HPV-18-transformed cell line), but not in C33A cells (a non-HPV-transformed cervical carcinoma cell line)...
  7. MECHANISM OF MOUSE LUNG CARCINOGENESIS
    Ming You; Fiscal Year: 2006
    ..In addition, wild type K-ras allele inhibited colony formation and tumor development of a transformed cell line that contained a mutant K-ras...
  8. BIOCHEMISTRY OF ANIMAL VIRUS MULTIPLICATION
    Maurice Green; Fiscal Year: 1980
    ..HE C19 cells, an Ad12 transformed cell line that appears to have an intact late region will be used to investigate regulation...
  9. Regulation of Oocyte Viability by Granulosa Cell Contact
    John Peluso; Fiscal Year: 2004
    ..abstract_text> ..
  10. PAIRBP & PGRMC1 act as a membrane receptor complex to mediate P4's ovarian action
    John J Peluso; Fiscal Year: 2010
    ..These new pharmacological agents could have utility in the development contraceptives and in treating infertility and some forms of cancer. ..
  11. PAIRBP & PGRMC1 act as a membrane receptor complex to mediate P4's ovarian action
    John Peluso; Fiscal Year: 2007
    ..These new pharmacological agents could have utility in the development contraceptives and in treating infertility and some forms of cancer. ..
  12. PAIRBP & PGRMC1 act as a membrane receptor complex to mediate P4's ovarian action
    John Peluso; Fiscal Year: 2009
    ..These new pharmacological agents could have utility in the development contraceptives and in treating infertility and some forms of cancer. ..
  13. PAIRBP & PGRMC1 act as a membrane receptor complex to mediate P4's ovarian action
    John J Peluso; Fiscal Year: 2010
    ..These new pharmacological agents could have utility in the development contraceptives and in treating infertility and some forms of cancer. ..
  14. Protein Kinase G Regulation of Granulosa Cell Viability
    John Peluso; Fiscal Year: 2006
    ..We now propose to test this hypothesis using biochemical, genetic, imaging and cell biological techniques. ..
  15. Progesterone regulation of human luteal cell viability
    John Peluso; Fiscal Year: 2006
    ..Such selective manipulation of the P4's actions could have far reaching effects on the treatment for infertility, contraception and certain types of cancers. ..
  16. Genetic Studies of Substance Abuse in Iowa Adoptees
    Robert Philibert; Fiscal Year: 2007
    ....
  17. HUMAN MAMMARY CELLS
    Martha Stampfer; Fiscal Year: 2002
    ..e. antisense telomerase RNA) activity. Additionally, the principal investigator will continue to collaborate with other investigators who wish to work with her cell lines. ..
  18. METALLOPROTEINASE EXPRESSION IN CORNEAL WOUNDS
    Dimitri Azar; Fiscal Year: 2002
    ..The study of MMPs and angiostatin in the cornea may provide valuable information about their behavior and possible clinical significance in other tissue. ..
  19. CORE GRANT FOR VISION RESEARCH
    Dimitri Azar; Fiscal Year: 2007
    ..abstract_text> ..
  20. Novel Mechanisms of Quinone Toxicity
    David Ross; Fiscal Year: 2010
    ..The studies will have broad applicability to a variety of organ systems. ..
  21. Fluid Secretion in Parotid Cells: Signal Transduction
    STEPHEN SOLTOFF; Fiscal Year: 2007
    ..We suggest that these areas of investigation will provide a more complete understanding of parotid cell function, and thereby contribute to future therapies to aid salivary dysfunction. ..
  22. BIOCHEMICAL AND MOLECULAR STUDIES ON DT DIAPHORASE
    David Ross; Fiscal Year: 2005
    ..The proposed studies represent an integrated chemical, biochemical and molecular approach to determine the significance of NQO1 and NQO1-directed antitumor agents in chemotherapy. ..
  23. BIOCHEMICAL AND MOLECULAR STUDIES ON DT DIAPHORASE
    David Ross; Fiscal Year: 2007
    ..Our studies will generate data that can be applied to ongoing and future clinical studies of RH1 and both benzoquinone and hydroquinone ansamycins. ..
  24. Transport of TNFalpha across the BBB
    Weihong Pan; Fiscal Year: 2007
    ..These transporters could be novel drug targets and therefore provide promising therapeutic potential. ..
  25. Early cellular immune responses to EBV
    Sumita Bhaduri McIntosh; Fiscal Year: 2007
    ..It is also expected to enhance the understanding of EBV lymphoproliferative diseases and have potential diagnostic and therapeutic implications. ..