transformed cell line

Summary

Summary: Eukaryotic cell line obtained in a quiescent or stationary phase which undergoes conversion to a state of unregulated growth in culture, resembling an in vitro tumor. It occurs spontaneously or through interaction with viruses, oncogenes, radiation, or drugs/chemicals.

Top Publications

  1. ncbi The Bloom's syndrome gene product interacts with topoisomerase III
    L Wu
    Imperial Cancer Research Fund Laboratories, Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford OX3 9DS, United Kingdom
    J Biol Chem 275:9636-44. 2000
  2. ncbi ATM and DNA-PK function redundantly to phosphorylate H2AX after exposure to ionizing radiation
    Tom Stiff
    Genome Damage and Stability Centre, University of Sussex, East Sussex, United Kingdom
    Cancer Res 64:2390-6. 2004
  3. ncbi Reconstitution of telomerase activity combined with HPV-E7 expression allow human preadipocytes to preserve their differentiation capacity after immortalization
    C Darimont
    Nestle Research Center, PO Box 44, Vers chez les Blanc, 1000 Lausanne 26, Switzerland
    Cell Death Differ 10:1025-31. 2003
  4. ncbi In vivo recruitment of XPC to UV-induced cyclobutane pyrimidine dimers by the DDB2 gene product
    Maureen E Fitch
    Departments of Medicine and Genetics, Division of Oncology, Stanford University School of Medicine, 269 Campus Drive, Stanford, CA 94305, USA
    J Biol Chem 278:46906-10. 2003
  5. ncbi Rapid induction of heparin-binding epidermal growth factor/diphtheria toxin receptor expression by Raf and Ras oncogenes
    S A McCarthy
    DNAX Research Institute, Palo Alto, California 94304, USA
    Genes Dev 9:1953-64. 1995
  6. pmc N-terminal domains of the human telomerase catalytic subunit required for enzyme activity in vivo
    B N Armbruster
    Department of Pharmacy and Cancer Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    Mol Cell Biol 21:7775-86. 2001
  7. ncbi RET/PTC1 oncogene signaling in PC Cl 3 thyroid cells requires the small GTP-binding protein Rho
    M V Barone
    Centro di Endocrinologia ed Oncologia Sperimentale del CNR, c/o Dipartimento di Biologia e Patologia Cellulare e Molecolare, , Via S. Pansini 5, Naples, Italy
    Oncogene 20:6973-82. 2001
  8. pmc Epstein-barr virus immediate-early protein BZLF1 is SUMO-1 modified and disrupts promyelocytic leukemia bodies
    A L Adamson
    Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 7295, USA
    J Virol 75:2388-99. 2001
  9. pmc Mutations in the phosphatidylinositol-3-kinase pathway predict for antitumor activity of the inhibitor PX-866 whereas oncogenic Ras is a dominant predictor for resistance
    Nathan T Ihle
    M D Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, TX 77030, USA
    Cancer Res 69:143-50. 2009
  10. ncbi Reversal of senescence in mouse fibroblasts through lentiviral suppression of p53
    Annette M G Dirac
    Division of Molecular Carcinogenesis, and Center for Biomedical Genetics, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    J Biol Chem 278:11731-4. 2003

Research Grants

  1. REGULATION OF TISSUE FACTOR PATHWAY INHIBITOR EXPRESSION
    MADHU BAJAJ; Fiscal Year: 2000
  2. RELATIONSHIPS BETWEEN PROSTANOIDS AND CELLULAR INJURY
    John Flynn; Fiscal Year: 2000
  3. CELL BIOLOGY OF PHOSPHATIDYLCHOLINE
    Susan Jaken; Fiscal Year: 1990
  4. HIV1 INHIBITORY PROTEINS FROM TRANSFORMED CD8+ CELLS
    Mary Klotman; Fiscal Year: 2002
  5. Anti-HPV RNA Interference Using Modified RNA's
    Kenneth Alexander; Fiscal Year: 2005
  6. THE FATE OF CYTOCHROME C IN CELL DEATH
    Ronald Jemmerson; Fiscal Year: 2000
  7. RESPONSE OF NEURONS TO ELECTRICAL FIELDS
    Kenneth Robinson; Fiscal Year: 1992
  8. MECHANISM OF MOUSE LUNG CARCINOGENESIS
    Ming You; Fiscal Year: 2006
  9. MECHANISMS OF REGULATION OF CELL PROLIFERATION
    Michael Getz; Fiscal Year: 1993
  10. PURIFICATION AND CHARACTERIZATION OF A TNF INHIBITOR
    Susan Wright; Fiscal Year: 1990

Detail Information

Publications251 found, 100 shown here

  1. ncbi The Bloom's syndrome gene product interacts with topoisomerase III
    L Wu
    Imperial Cancer Research Fund Laboratories, Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford OX3 9DS, United Kingdom
    J Biol Chem 275:9636-44. 2000
    ..Furthermore, through characterizing a genetic interaction between BLM and TOP3 in S. cerevisiae, we have identified a functional role for the hTOPO IIIalpha interaction domains in BLM...
  2. ncbi ATM and DNA-PK function redundantly to phosphorylate H2AX after exposure to ionizing radiation
    Tom Stiff
    Genome Damage and Stability Centre, University of Sussex, East Sussex, United Kingdom
    Cancer Res 64:2390-6. 2004
    ..However, by phosphorylating H2AX, DNA-PK can contribute to the presence of the damage response proteins MDC1 and 53BP1 at the site of the DSB...
  3. ncbi Reconstitution of telomerase activity combined with HPV-E7 expression allow human preadipocytes to preserve their differentiation capacity after immortalization
    C Darimont
    Nestle Research Center, PO Box 44, Vers chez les Blanc, 1000 Lausanne 26, Switzerland
    Cell Death Differ 10:1025-31. 2003
    ..These data demonstrate, for the first time, that coexpression of hTERT and HPV-E7 in human preadipocytes allows cells not only to display an indefinite life span but also to retain their capacity to differentiate...
  4. ncbi In vivo recruitment of XPC to UV-induced cyclobutane pyrimidine dimers by the DDB2 gene product
    Maureen E Fitch
    Departments of Medicine and Genetics, Division of Oncology, Stanford University School of Medicine, 269 Campus Drive, Stanford, CA 94305, USA
    J Biol Chem 278:46906-10. 2003
    ..XPC did localize to CPDs when p48 was overexpressed in the same cell, signifying that p48 activates the recruitment of XPC to CPDs and may be the initial recognition factor in the NER pathway...
  5. ncbi Rapid induction of heparin-binding epidermal growth factor/diphtheria toxin receptor expression by Raf and Ras oncogenes
    S A McCarthy
    DNAX Research Institute, Palo Alto, California 94304, USA
    Genes Dev 9:1953-64. 1995
    ....
  6. pmc N-terminal domains of the human telomerase catalytic subunit required for enzyme activity in vivo
    B N Armbruster
    Department of Pharmacy and Cancer Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    Mol Cell Biol 21:7775-86. 2001
    ..The discovery of these domains provides the first step in dissecting the biological functions of human telomerase, with the ultimate goal of targeting this enzyme for the treatment of human cancers...
  7. ncbi RET/PTC1 oncogene signaling in PC Cl 3 thyroid cells requires the small GTP-binding protein Rho
    M V Barone
    Centro di Endocrinologia ed Oncologia Sperimentale del CNR, c/o Dipartimento di Biologia e Patologia Cellulare e Molecolare, , Via S. Pansini 5, Naples, Italy
    Oncogene 20:6973-82. 2001
    ..We conclude that Rho is implicated in the actin reorganization and cell survival mediated by the chimeric RET/PTC1 oncogene in thyroid epithelial cells, both phenotypes being cell type- and oncogene type-specific...
  8. pmc Epstein-barr virus immediate-early protein BZLF1 is SUMO-1 modified and disrupts promyelocytic leukemia bodies
    A L Adamson
    Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 7295, USA
    J Virol 75:2388-99. 2001
    ..These results suggest that disruption of PML bodies is important for efficient lytic replication of EBV. Furthermore, Z may potentially alter the function of a variety of cellular proteins by inhibiting SUMO-1 modification...
  9. pmc Mutations in the phosphatidylinositol-3-kinase pathway predict for antitumor activity of the inhibitor PX-866 whereas oncogenic Ras is a dominant predictor for resistance
    Nathan T Ihle
    M D Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, TX 77030, USA
    Cancer Res 69:143-50. 2009
    ....
  10. ncbi Reversal of senescence in mouse fibroblasts through lentiviral suppression of p53
    Annette M G Dirac
    Division of Molecular Carcinogenesis, and Center for Biomedical Genetics, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    J Biol Chem 278:11731-4. 2003
    ..These data indicate that senescence in MEFs is reversible and demonstrate that both initiation and maintenance of senescence is p53-dependent...
  11. pmc Production of high-titer recombinant adeno-associated virus vectors in the absence of helper adenovirus
    X Xiao
    Gene Therapy Center, Division of Pharmaceutics, University of North Carolina at Chapel Hill, 27599, USA
    J Virol 72:2224-32. 1998
    ....
  12. pmc Measurements of the BKCa channel's high-affinity Ca2+ binding constants: effects of membrane voltage
    Tara Beth Sweet
    Molecular Cardiology Research Institute, Tufts Medical Center, Tufts University School of Medicine, Boston, MA 02111, USA
    J Gen Physiol 132:491-505. 2008
    ....
  13. ncbi Role of PPAR-gamma ligands in neuroprotection against glutamate-induced cytotoxicity in retinal ganglion cells
    Paul Aoun
    Department of Pharmacology and Neuroscience, University of North Texas Health Science Center, Fort Worth, Texas 76107, USA
    Invest Ophthalmol Vis Sci 44:2999-3004. 2003
    ..Glaucoma and other retinal disorders are some of the major complications in diabetes. In the present study, the role that PPAR-gamma ligands play in protecting retinal ganglion cells (RGC-5) against glutamate insult was explored...
  14. pmc Three functional variants of IFN regulatory factor 5 (IRF5) define risk and protective haplotypes for human lupus
    Robert R Graham
    Program in Medical and Population Genetics, Broad Institute of Harvard and Massachusetts Institute of Technology, Cambridge, MA 02142, USA
    Proc Natl Acad Sci U S A 104:6758-63. 2007
    ....
  15. ncbi Matricellular protein SPARC is translocated to the nuclei of immortalized murine lens epithelial cells
    Qi Yan
    Hope Heart Program, Benaroya Research Institute at Virginia Mason, Seattle, Washington 98104 2046, USA
    J Cell Physiol 203:286-94. 2005
    ..SPARC therefore functions not only as an extracellular protein but also potentially as an intracellular protein to influence cellular activities and homeostasis...
  16. pmc HTLV-I basic leucine zipper factor gene mRNA supports proliferation of adult T cell leukemia cells
    Yorifumi Satou
    Laboratory of Virus Immunology, Institute for Virus Research, Kyoto University, 53 Shogoin Kawahara cho, Sakyo ku, Kyoto 606 8507, Japan
    Proc Natl Acad Sci U S A 103:720-5. 2006
    ..Thus, the single HBZ gene has bimodal functions in two different molecular forms. The growth-promoting activity of HBZ RNA likely plays an important role in oncogenesis by HTLV-I...
  17. ncbi PAK4 functions in tumor necrosis factor (TNF) alpha-induced survival pathways by facilitating TRADD binding to the TNF receptor
    Xiaofan Li
    Department of Biological Sciences, Columbia University, New York, New York 10027, USA
    J Biol Chem 280:41192-200. 2005
    ..The role for PAK4 in regulating prosurvival pathways is a completely new function for this protein, and the connection between PAK4 and cell survival under stress helps explain its role in tumorigenesis and development...
  18. ncbi Combined activation of Ras and Akt in neural progenitors induces glioblastoma formation in mice
    E C Holland
    Department of Neurosurgery, MD Anderson Cancer Center, Houston, Texas, USA
    Nat Genet 25:55-7. 2000
    ..11), and we show here that Akt activity is increased in most of these tumours, implying that combined activation of these two pathways accurately models the biology of this disease...
  19. ncbi The mechanisms controlling the recognition of tumor- and virus-infected cells by NKp46
    Tal I Arnon
    Lautenberg Center for General and Tumor Immunology, The Hebrew University Hadassah Medical School, Jerusalem, 91120, Israel
    Blood 103:664-72. 2004
    ..These results may explain the ability of NK cells to kill such a broad spectrum of viral-infected and tumor cells...
  20. ncbi Mitochondrial repair of 8-oxoguanine is deficient in Cockayne syndrome group B
    Tinna Stevnsner
    Danish Center for Molecular Gerontology, Department of Molecular Biology, University of Aarhus, DK 8000 Aarhus C, Denmark
    Oncogene 21:8675-82. 2002
    ..For the first time we report that CSB stimulates repair of 8-oxoG in mammalian mitochondrial DNA. Furthermore, evidence is presented to support the hypothesis that wild type CSB regulates expression of OGG1...
  21. pmc LPP, an actin cytoskeleton protein related to zyxin, harbors a nuclear export signal and transcriptional activation capacity
    M M Petit
    Laboratory for Molecular Oncology, Center for Human Genetics, University of Leuven and Flanders Interuniversity Institute for Biotechnology, B 3000 Leuven, Belgium
    Mol Biol Cell 11:117-29. 2000
    ..Altogether, these results show that the LPP protein has multifunctional domains and may serve as a scaffold upon which distinct protein complexes are assembled in the cytoplasm and in the nucleus...
  22. ncbi Cloning and characterization of the 5'-flanking region of the human transcription factor Sp1 gene
    M Nicolas
    Department of Biochemistry and Molecular Biology, School of Pharmacy, University of Barcelona, 08028 Barcelona, Spain
    J Biol Chem 276:22126-32. 2001
    ..Sp1 and Sp3 proteins bind to the downstream GC box located in the proximal promoter of Sp1. Furthermore, we demonstrate that the Sp1 protein activates Sp1 transcription activity; thus the Sp1 gene is autoregulated...
  23. pmc Epithelial uptake and transport of cell-free human immunodeficiency virus type 1 and gp120-coated microparticles
    A Kage
    Universitatsklinikum Rudolf Virchow, Institut fur Klinische Chemie und Biochemie, Berlin, Germany
    J Virol 72:4231-6. 1998
    ..Penetration of HIV could be inhibited by soluble glycoconjugates present in oral mucins...
  24. ncbi Acid production in glycolysis-impaired tumors provides new insights into tumor metabolism
    Gabriel Helmlinger
    Department of Radiation Oncology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02114, USA
    Clin Cancer Res 8:1284-91. 2002
    ..In this study, we tested the hypothesis that lactate is not the only source of acidification in solid tumors and explored the potential mechanisms underlying these often-observed high rates of acid production...
  25. ncbi Cell surface phenotyping and cytokine production of Epstein-Barr Virus (EBV)-transformed lymphoblastoid cell lines (LCLs)
    Joanne M Wroblewski
    Division of Hematology and Oncology, College of Medicine, University of Kentucky, Lexington, KY 40536, USA
    J Immunol Methods 264:19-28. 2002
    ..Together, these data reflect the heterogeneity of EBV-LCLs, which cautions their use nondiscriminately in various immunologic assays...
  26. ncbi Subnuclear compartmentalization of immunoglobulin loci during lymphocyte development
    Steven T Kosak
    Department of Molecular Genetics and Cell Biology, Howard Hughes Medical Institute, University of Chicago, Chicago, IL 60637, USA
    Science 296:158-62. 2002
    ..We suggest that subnuclear positioning represents a novel means of regulating transcription and recombination of IgH and Igkappa loci during lymphocyte development...
  27. ncbi Immortalization of human CD8+ T cell clones by ectopic expression of telomerase reverse transcriptase
    E Hooijberg
    Department of Immunology, The Netherlands Cancer Institute Antoni van Leeuwenhoek Hospital
    J Immunol 165:4239-45. 2000
    ..As the T cells remained dependent on cytokines and Ag stimulation for their in vitro expansion, we conclude that immortalization was achieved without malignant transformation...
  28. ncbi A proline- and glutamine-rich protein promotes apoptosis in neuronal cells
    I Gomes
    Department of Medicine, University of Illinois at Chicago, 60637, USA
    J Neurochem 73:612-22. 1999
    ..Furthermore, these data suggest that PQR can function more generally as a mediator of apoptosis and provide a possible mechanism for induction of programmed cell death during neuronal development...
  29. pmc Relationship between contact inhibition and intranuclear S100C of normal human fibroblasts
    M Sakaguchi
    Department of Cell Biology, Institute of Molecular and Cellular Biology, Okayama University Medical School, Okayama 700 8558, Japan
    J Cell Biol 149:1193-206. 2000
    ..These data indicate the possible involvement of nuclear S100C in the contact inhibition of cell growth...
  30. ncbi Association of gp91phox homolog Nox1 with anchorage-independent growth and MAP kinase-activation of transformed human keratinocytes
    Walee Chamulitrat
    Deutsches Krebsforschungszentrum, Department of Applied Tumorvirology, 69120 Heidelberg, Germany
    Oncogene 22:6045-53. 2003
    ..Furthermore, JNK2 was constitutively phosphorylated in FIB cells. Together, our data strongly implicate Nox1 in redox-mediated signaling related to cellular activation of human keratinocytes at a more advanced stage of transformation...
  31. ncbi Catalytically active human telomerase mutants with allele-specific biological properties
    Moses Kim
    Department of Biochemistry and Biophysics, University of California at San Francisco, San Francisco, CA 94143 2200, USA
    Exp Cell Res 288:277-87. 2003
    ..These results uncover further hTERT allele-specific phenotypes that uncouple telomerase activity, net telomere lengthening and life span extension...
  32. ncbi Altered apoptotic gene expression and acquired apoptotic resistance in cadmium-transformed human prostate epithelial cells
    William E Achanzar
    Inorganic Carcinogenesis Section, National Cancer Institute at National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA
    Prostate 52:236-44. 2002
    ..To investigate these mechanisms, we performed molecular comparisons between the cadmium-transformed prostate epithelial cell line CTPE and the nontumorigenic parental line RWPE-1...
  33. ncbi Requirement of mammalian DNA polymerase-beta in base-excision repair
    R W Sobol
    Sealy Center for Molecular Science, University of Texas Medical Branch, Galveston 77555 1068, USA
    Nature 379:183-6. 1996
    ..These studies demonstrate that beta-polymerase functions specifically in base-excision repair in vivo...
  34. pmc E2f3 is critical for normal cellular proliferation
    P O Humbert
    Center for Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139 USA
    Genes Dev 14:690-703. 2000
    ..We conclude that E2F3 is critical for the transcriptional activation of genes that control the rate of proliferation of both primary and tumor cells...
  35. ncbi DDB accumulates at DNA damage sites immediately after UV irradiation and directly stimulates nucleotide excision repair
    Mitsuo Wakasugi
    Faculty of Pharmaceutical Sciences, Kanazawa University, Takara machi, Kanazawa 920 0934, Japan
    J Biol Chem 277:1637-40. 2002
    ..These results suggest that DDB can rapidly translocate to the damaged DNA sites independent of functional XPA and XPC proteins and directly enhance the excision reaction by core repair factors...
  36. ncbi Mlx, a new Max-like bHLHZip family member: the center stage of a novel transcription factors regulatory pathway?
    G Meroni
    Telethon Institute of Genetics and Medicine, Milan, Italy
    Oncogene 19:3266-77. 2000
    ..The possible role of Mlx in an emerging regulatory pathway and acting parallel to the Max driven network is discussed...
  37. ncbi Specific packaging of APOBEC3G into HIV-1 virions is mediated by the nucleocapsid domain of the gag polyprotein precursor
    Alexandra Schäfer
    Howard Hughes Medical Institute and Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC 27710, USA
    Virology 328:163-8. 2004
    ..Surprisingly, RNA was also found to be essential for formation of the nucleocapsid--APOBEC3G complex in vitro, thus raising the possibility that RNA may form a bridge between these two proteins...
  38. ncbi Clonal heterogeneity in differentiation potential of immortalized human mesenchymal stem cells
    Takeshi Okamoto
    Department of Tissue Regeneration, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara cho, Shogoin, Sakyo ku, Kyoto 606 8057, Japan
    Biochem Biophys Res Commun 295:354-61. 2002
    ..These cell lines, being the first established immortalized clonal cell lines of hMSCs, could provide insights into the mechanisms regulating the early steps of differentiation from undifferentiated MSCs into a specific lineage...
  39. pmc Quantitative and functional analyses of spastin in the nervous system: implications for hereditary spastic paraplegia
    Joanna M Solowska
    Department of Neurobiology and Anatomy, Drexel University College of Medicine, Philadelphia, Pennsylvania 19129, USA
    J Neurosci 28:2147-57. 2008
    ..These results are consistent with a "gain-of-function" mechanism underlying HSP wherein spastin mutations produce a cytotoxic protein in the case of M1 but not M85...
  40. ncbi Brain transplantation of immortalized human neural stem cells promotes functional recovery in mouse intracerebral hemorrhage stroke model
    Hong J Lee
    Division of Neurology, Department of Medicine, University of British Columbia Hospital, University of British Columbia, Vancouver, British Columbia V6T 2B5, Canada
    Stem Cells 25:1204-12. 2007
    ..Disclosure of potential conflicts of interest is found at the end of this article...
  41. pmc Involvement of ER stress in retinal cell death
    Masamitsu Shimazawa
    Department of Biofunctional Molecules, Gifu Pharmaceutical University, Gifu, Japan
    Mol Vis 13:578-87. 2007
    ....
  42. ncbi Inhibition of Egr-1 expression reverses transformation of prostate cancer cells in vitro and in vivo
    Veronique Baron
    Sidney Kimmel Cancer Center, 10835 Altman Row, San Diego, CA 92121, USA
    Oncogene 22:4194-204. 2003
    ..026). Thus, Egr-1 plays a functional role in the transformed phenotype and may represent a valid target for prostate cancer therapy...
  43. ncbi Gliotoxin-mediated apoptosis of activated human hepatic stellate cells
    Young Oh Kweon
    Division of Digestive Diseases and Nutrition, Department of Medicine, CB 7038, Glaxo Research Bldg Rm 156, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    J Hepatol 39:38-46. 2003
    ..Gliotoxin induces apoptosis of activated human and rat HSCs by an unknown mechanism...
  44. ncbi The adaptor protein fish associates with members of the ADAMs family and localizes to podosomes of Src-transformed cells
    Clare L Abram
    SUGEN Inc, South San Francisco, California 94080, USA
    J Biol Chem 278:16844-51. 2003
    ..Because members of the ADAMs family have been implicated in growth factor processing, as well as cell adhesion and motility, Fish could be acting as an adaptor molecule that allows Src to impinge on these processes...
  45. pmc A new family of genes coding for an antigen recognized by autologous cytolytic T lymphocytes on a human melanoma
    B Van den Eynde
    Ludwig Institute for Cancer Research, Brussels Branch, Belgium
    J Exp Med 182:689-98. 1995
    ..About 50% of melanoma patients carry on their tumor at least one of the presently defined antigens encoded by the MAGE, BAGE, and GAGE genes...
  46. pmc IAPs block apoptotic events induced by caspase-8 and cytochrome c by direct inhibition of distinct caspases
    Q L Deveraux
    The Burnham Institute, Program on Apoptosis and Cell Death Research, 10901 N Torrey Pines Road, La Jolla, CA 92037, USA
    EMBO J 17:2215-23. 1998
    ..These findings demonstrate that IAPs can suppress different apoptotic pathways by inhibiting distinct caspases and identify pro-caspase-9 as a new target for IAP-mediated inhibition of apoptosis...
  47. ncbi The vimentin cytoskeleton regulates focal contact size and adhesion of endothelial cells subjected to shear stress
    Daisuke Tsuruta
    Department of Cell and Molecular Biology, Northwestern University Medical School, Morton 4 616, 303 E Chicago Avenue, Chicago, IL 60611, USA
    J Cell Sci 116:4977-84. 2003
    ..More dramatically, such cells showed decreased adhesion to the substratum. These data provide evidence that the vimentin cytoskeleton regulates focal contact size and helps stabilize cell-matrix adhesions in endothelial cells...
  48. ncbi HIV-1 Gag protein associates with F-actin present in microfilaments
    O Rey
    Department of Pediatrics, School of Medicine, University of California at Los Angeles 90095, USA
    Virology 220:530-4. 1996
    ..In vivo and in vitro analyses of this interaction indicated that the unprocessed Gag polyprotein is capable of association with polymerized actin (F-actin). Binding of Gag to F-actin may be involved in the assembly or budding of HIV-1...
  49. ncbi Cultivation of an immortalized human corneal endothelial cell population and two distinct clonal subpopulations on thermo-responsive carriers
    Thomas Götze
    Leibniz Institute of Polymer Research Dresden, Max Bergmann Center of Biomaterials, Hohe Strasse 6, 01069, Dresden, Germany
    Graefes Arch Clin Exp Ophthalmol 246:1575-83. 2008
    ..We sought to study HCEC sheet cultivation on such cell culture carriers under serum-free conditions as the next consequential step in developing methods for generation of corneal endothelial cell transplants...
  50. ncbi A conditionally immortalized human podocyte cell line demonstrating nephrin and podocin expression
    Moin A Saleem
    Children s Renal Unit and Academic Renal Unit, University of Bristol, Southmead Hospital, Bristol, UK
    J Am Soc Nephrol 13:630-8. 2002
    ..In conclusion, the development of this cell line provides a new tool in the study of podocyte biology, which will enable accurate assessment of the behavior of these complex cells in health and disease...
  51. ncbi UV-induced ubiquitylation of XPC protein mediated by UV-DDB-ubiquitin ligase complex
    Kaoru Sugasawa
    Cellular Physiology Laboratory, RIKEN Discovery Research Institute, Japan Science and Technology Agency, Wako, Saitama 351 0198, Japan
    Cell 121:387-400. 2005
    ..Our results strongly suggest that ubiquitylation plays a critical role in the transfer of the UV-induced lesion from UV-DDB to XPC...
  52. ncbi Identification of the Abl- and rasGAP-associated 62 kDa protein as a docking protein, Dok
    Y Yamanashi
    Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA
    Cell 88:205-11. 1997
    ..A monoclonal antibody, 2C4, to the rasGAP-associated p62 reacts with p62dok. Thus, p62dok appears to be the long-sought major substrate of many tyrosine kinases...
  53. ncbi Spontaneously immortalized human T lymphocytes develop gain of chromosomal region 2p13-24 as an early and common genetic event
    Jan Konrad Siwicki
    Department of Immunology, Maria Skłodowska Curie Memorial Cancer Center and Institute of Oncology, Warsaw, Poland
    Genes Chromosomes Cancer 41:133-44. 2004
    ....
  54. ncbi c-Myc directly regulates the transcription of the NBS1 gene involved in DNA double-strand break repair
    Yu Chi Chiang
    Department of Medical Genetics, College of Medicine, National Taiwan University, Taipei 100, Taiwan
    J Biol Chem 278:19286-91. 2003
    ..These results indicate that NBS1 is a direct transcriptional target of c-Myc and links the function of c-Myc to the regulation of DNA DSB repair pathway operating during DNA replication...
  55. ncbi Maintenance of G1 checkpoint controls in telomerase-immortalized endothelial cells
    Deborah A Freedman
    Vascular Biology Program, Children s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
    Cell Cycle 3:811-6. 2004
    ..This result could have significant implications in the study of angiogenic processes such as tumor growth, wound healing, and the vascularization of engineered tissue...
  56. ncbi Clusterin (SGP-2) transient overexpression decreases proliferation rate of SV40-immortalized human prostate epithelial cells by slowing down cell cycle progression
    Saverio Bettuzzi
    Dipartimento di Medicina Sperimentale, Universita di Parma, Via Volturno, 39 43100 Parma, Italy
    Oncogene 21:4328-34. 2002
    ....
  57. ncbi Characterization and tumorigenicity of human ovarian surface epithelial cells immortalized by SV40 large T antigen
    M Nitta
    Department of Obstetrics and Gynecology, Kumamoto University School of Medicine, Kumamoto 860-8556, Japan
    Gynecol Oncol 81:10-7. 2001
    ..CONCLUSIONS: The established cell lines may provide a model system to investigate the mechanisms of cytogenic and molecular changes from normal OSE cells through the various steps of transformation...
  58. ncbi Proactive transplantation of human neural stem cells prevents degeneration of striatal neurons in a rat model of Huntington disease
    Jae K Ryu
    Brain Disease Research Center, Ajou University School of Medicine, Suwon, South Korea
    Neurobiol Dis 16:68-77. 2004
    ..The findings in this work have particular relevance to a rat model of HD in that proactive transplanted hNSCs protect host striatal neurons against neuronal injury and improve motor impairment induced by 3-NP toxicity...
  59. ncbi Porphyromonas gingivalis selectively up-regulates the HIV-1 coreceptor CCR5 in oral keratinocytes
    Rodrigo A Giacaman
    Department of Diagnostic and Biological Sciences, School of Dentistry, University of Minnesota, Minneapolis, MN 55455, USA
    J Immunol 179:2542-50. 2007
    ..By inducing CCR5 expression, P. gingivalis coinfection could promote selective R5-type HIV-1 infection of oral keratinocytes...
  60. ncbi Allosteric inhibitors of Bcr-abl-dependent cell proliferation
    Francisco J Adrian
    Biological Chemistry Department, Genomics Institute of the Novartis Research Foundation, 10675 John Jay Hopkins Drive, San Diego, California 92121, USA
    Nat Chem Biol 2:95-102. 2006
    ..We propose that this new class of compounds inhibits Bcr-abl kinase activity through an allosteric non-ATP competitive mechanism...
  61. ncbi Epstein-Barr virus-encoded latent membrane protein 1 (LMP1) induces the expression of the cellular microRNA miR-146a
    Natalie Motsch
    Institute of Virology, University of Saarland Medical School, Homburg, Germany
    RNA Biol 4:131-7. 2007
    ..It is possible that the induction of miR-146a plays a role in the induction or maintenance of EBV latency by modulating innate immune responses to the virus infected host cell...
  62. ncbi Identification of MIP-1 alpha/LD78 as a monocyte chemoattractant released by the HTLV-I-transformed cell line MT4
    R Bertini
    Laboratories of Biotechnology, Research Center Dompé S p A, L Aquila, Italy
    AIDS Res Hum Retroviruses 11:155-60. 1995
    It is known that the HTLV-I-transformed cell line MT4 releases chemotactic activity for monocytes spontaneously...
  63. ncbi Conditionally immortalized human glomerular endothelial cells expressing fenestrations in response to VEGF
    S C Satchell
    Academic Renal Unit, University of Bristol, Southmead Hospital, Bristol, UK
    Kidney Int 69:1633-40. 2006
    ..They express fenestrations which are upregulated in response to VEGF. These cells are a unique resource for further study of GEnC and their roles in glomerular filtration, glomerular disease, and response to glomerular injury...
  64. ncbi E-cadherin-mediated cell contact prevents apoptosis of spontaneously immortalized granulosa cells by regulating Akt kinase activity
    J J Peluso
    Departments of Physiology and Obstetrics and Gynecology, University of Connecticut Health Center, Farmington, Connecticut 06030, USA
    Biol Reprod 64:1183-90. 2001
    ....
  65. pmc Perk-dependent translational regulation promotes tumor cell adaptation and angiogenesis in response to hypoxic stress
    Jaime D Blais
    Ottawa Health Research Institute, Ottawa, ON K1H 1C4, Canada
    Mol Cell Biol 26:9517-32. 2006
    ....
  66. pmc Lytic induction therapy for Epstein-Barr virus-positive B-cell lymphomas
    Wen hai Feng
    Department of Medicine, and Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    J Virol 78:1893-902. 2004
    ..These results suggest that the addition of GCV to either gemcitabine- or doxorubicin-containing chemotherapy regimens may enhance the therapeutic efficacy of these drugs for EBV-driven lymphoproliferative disease in patients...
  67. ncbi Generation and characterization of immortalized human microglial cell lines: expression of cytokines and chemokines
    A Nagai
    Division of Neurology, Department of Medicine, University of British Columbia, Vancouver, Canada
    Neurobiol Dis 8:1057-68. 2001
    ..The human microglial cell line HMO6 exhibits similar properties to those documented in human microglia and should have considerable utility as an in vitro model for the studies of human microglia in health and disease...
  68. ncbi BLM, the Bloom's syndrome protein, varies during the cell cycle in its amount, distribution, and co-localization with other nuclear proteins
    M M Sanz
    Laboratory of Human Genetics, Department of Pediatrics, Weill Medical College of Cornell University, New York, NY 10021, USA
    Cytogenet Cell Genet 91:217-23. 2000
    ....
  69. ncbi Recycling compartments and the internal vesicles of multivesicular bodies harbor most of the cholesterol found in the endocytic pathway
    W Mobius
    Department of Cell Biology and Institute of Biomembranes and Center for Biomedical Genetics, University Medical Center Utrecht, The Netherlands
    Traffic 4:222-31. 2003
    ..Thus, cholesterol displays a highly differential distribution in the various membrane domains of the endocytic pathway...
  70. ncbi Inhibition of protein synthesis by the T cell receptor-inducible human TDAG51 gene product
    T Hinz
    Department of Immunology, Paul Ehrlich Institute, Paul Ehrlich Stasse 51 59, D 63225, Langen, Germany
    Cell Signal 13:345-52. 2001
    ..We speculate that hTDAG51 couples TCR signaling to inhibition of protein biosynthesis in activated T lymphocytes...
  71. ncbi Titanium dioxide (P25) produces reactive oxygen species in immortalized brain microglia (BV2): implications for nanoparticle neurotoxicity
    Thomas C Long
    Department of Environmental Sciences and Engineering, School of Public Health, University of North Carolina, Chapel Hill, North Carolina 27599 7431, USA
    Environ Sci Technol 40:4346-52. 2006
    ..Cell viability was maintained at all test concentrations (2.5-120 ppm) over the 18 h exposure period. These data indicate that mouse microglia respond to Degussa P25 with cellular and morphological expressions of free radical formation...
  72. ncbi Akt deficiency impairs normal cell proliferation and suppresses oncogenesis in a p53-independent and mTORC1-dependent manner
    Jennifer E Skeen
    Department of Biochemistry and Molecular Genetics, University of Illinois, Chicago, Illinois 60607, USA
    Cancer Cell 10:269-80. 2006
    ..Surprisingly, upon mTORC1 hyperactivation, the reduction in Akt activity does not impair cell proliferation and susceptibility to oncogenic transformation; thus, Akt may mediate these processes exclusively via mTORC1...
  73. ncbi Convergence of the fanconi anemia and ataxia telangiectasia signaling pathways
    Toshiyasu Taniguchi
    Department of Pediatric Oncology, Dana Farber Cancer Institute and Department of Pediatrics, Children s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Cell 109:459-72. 2002
    ..Biallelic disruption of FANCD2 results in both MMC and IR hypersensitivity...
  74. ncbi A mechanism in Epstein-Barr virus oncogenesis: inhibition of transforming growth factor-beta 1-mediated induction of MAPK/p21 by LMP1
    Makoto Fukuda
    Department of Biosignaling, School of Life Science, Faculty of Medicine, Tottori University, Yonago 683 8505, Japan
    Virology 302:310-20. 2002
    ....
  75. ncbi Formation of higher-order nuclear Rad51 structures is functionally linked to p21 expression and protection from DNA damage-induced apoptosis
    Elke Raderschall
    Max Planck Institute of Molecular Genetics, 14195 Berlin, Germany
    J Cell Sci 115:153-64. 2002
    ..This mechanism may contribute to a highly effective recombinational DNA repair in cell cycle-arrested cells and protection against DNA damage-induced apoptosis...
  76. pmc Genomic alterations link Rho family of GTPases to the highly invasive phenotype of pancreas cancer
    Alec C Kimmelman
    Department of Medical Oncology, Dana Farber Cancer Institute and Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 105:19372-7. 2008
    ..Like RIOK3, PAK4 promotes pancreas ductal cell motility and invasion. Together, the genomic and functional profiles establish the Rho family GTP-binding proteins as integral to the hallmark invasive nature of this lethal disease...
  77. pmc Identification of a novel element involved in regulation of the lytic switch BZLF1 gene promoter of Epstein-Barr virus
    R J Kraus
    McArdle Laboratory for Cancer Research, University of Wisconsin Medical School, Madison, Wisconsin 53706-1599, USA
    J Virol 75:867-77. 2001
    ..We conclude that ZV functions as an important regulatory element of the BZLF1 promoter, with ZVR likely playing important roles in the maintenance of latency and reactivation of EBV...
  78. pmc Hemidesmosome formation is initiated by the beta4 integrin subunit, requires complex formation of beta4 and HD1/plectin, and involves a direct interaction between beta4 and the bullous pemphigoid antigen 180
    R Q Schaapveld
    Division of Cell Biology, The Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands
    J Cell Biol 142:271-84. 1998
    ..Unfolding of the cytoplasmic domain may be part of a mechanism by which the interaction of beta4 with other hemidesmosomal components, e.g., BP180, is regulated...
  79. pmc Developmentally regulated recruitment of transcription factors and chromatin modification activities to chicken lysozyme cis-regulatory elements in vivo
    Pascal Lefevre
    Molecular Medicine Unit, University of Leeds, St James s University Hospital, Leeds LS9 7TF, United Kingdom
    Mol Cell Biol 23:4386-400. 2003
    ..This indicates that a partially accessible chromatin structure of lineage-specific genes is a hallmark of hematopoietic progenitor cells...
  80. pmc Recruitment of a protein complex containing Tat and cyclin T1 to TAR governs the species specificity of HIV-1 Tat
    P D Bieniasz
    Howard Hughes Medical Institute and Department of Genetics, Box 3025, Room 426, CARL Building, Duke University Medical Center, Research Drive, Durham, NC 27710, USA
    EMBO J 17:7056-65. 1998
    ....
  81. ncbi Nephrotic plasma alters slit diaphragm-dependent signaling and translocates nephrin, Podocin, and CD2 associated protein in cultured human podocytes
    Richard J M Coward
    Academic and Children s Renal Unit, Southmead Hospital, University of Bristol, Bristol, UK
    J Am Soc Nephrol 16:629-37. 2005
    ..This work demonstrates that nephrotic plasma seems to be deficient in factors that act via the podocyte SD complex, which are essential in maintaining its physiologic function...
  82. ncbi Extracellular matrix-associated serine protease inhibitors (Mr 33,000, 31,000, and 27,000) are single-gene products with differential glycosylation: cDNA cloning of the 33-kDa inhibitor reveals its identity to tissue factor pathway inhibitor-2
    C N Rao
    Department of Dermatology, Northwestern University School of Medicine, Chicago, Illinois 60611 3008, USA
    Arch Biochem Biophys 335:82-92. 1996
    ..None or very little of the inhibitors (0-2%) remained in a conditioned medium. Because they are primarily associated with ECM, the inhibitors may play a major role in ECM remodeling and turnover...
  83. pmc Multiple centrosomes arise from tetraploidy checkpoint failure and mitotic centrosome clusters in p53 and RB pocket protein-compromised cells
    Franck Borel
    Institut de Biologie Structurale Jean Pierre Ebel, Commissariat à l Energie Atomique Centre National de la Recherche Scientifique, 41 rue Jules Horowitz, 38027 Grenoble Cedex 1, France
    Proc Natl Acad Sci U S A 99:9819-24. 2002
    ..Finally, we find that mouse embryo fibroblasts spontaneously enter tetraploid G(1), explaining the previous demonstration of centrosome amplification by p53 abrogation alone in these cells...
  84. ncbi DNA methyltransferase 3b contributes to oncogenic transformation induced by SV40T antigen and activated Ras
    Kenzo Soejima
    Department of Medical Oncology, Dana Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA
    Oncogene 22:4723-33. 2003
    ..Regardless of mechanism, our data indicate that DNMT3b plays an important role in transformation...
  85. ncbi Transforming growth factor-beta1 promotes invasiveness after cellular transformation with activated Ras in intestinal epithelial cells
    K Fujimoto
    Department of Surgery, Vanderbilt University Medical Center and Vanderbilt-Ingram Cancer Center, Nashville, Tennessee 37232, USA
    Exp Cell Res 266:239-49. 2001
    ..The enhancement of invasiveness was correlated with decreased E-cadherin levels and subcellular distribution of beta-catenin. The enhancement of oncogenic Ras-mediated cell transformation by TGF-beta1 occurs via TbetaRII...
  86. ncbi Importance of poly(ADP-ribose) polymerase and its cleavage in apoptosis. Lesson from an uncleavable mutant
    F J Oliver
    UPR 9003 du Centre National de la Recherche Scientifique Cancérogenèse et Mutagenèse Moléculaire et Structurale, Laboratoire Conventionné, Illkirch Graffenstaden, France
    J Biol Chem 273:33533-9. 1998
    ....
  87. ncbi Expression of gene rrg is associated with reversion of NIH 3T3 transformed by LTR-c-H-ras
    S Contente
    Department of Pathology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814 4799
    Science 249:796-8. 1990
    ..Stable transfection with sense constructs did not alter the normal morphology, message level, or nontumorigenicity of the persistent revertant cell line...
  88. ncbi Ras transformation requires metabolic control by 6-phosphofructo-2-kinase
    S Telang
    Molecular Targets Program, James Graham Brown Cancer Center, University of Louisville, Louisville, KY 40202, USA
    Oncogene 25:7225-34. 2006
    ....
  89. pmc c-Myc and Rel/NF-kappaB are the two master transcriptional systems activated in the latency III program of Epstein-Barr virus-immortalized B cells
    Nathalie Faumont
    Centre National de la Recherche Scientifique, Unité Mixte de Recherche 6101, Centre Hospitalier Universitaire Dupuytren, Universite de Limoges, Laboratoire d Hematologie, 87025 Limoges, France
    J Virol 83:5014-27. 2009
    ..Thus, c-Myc and NF-kappaB are the two main transcription factors responsible for the phenotype, growth pattern, and biological properties of cells driven into proliferation by EBV...
  90. pmc Impact of the central polypurine tract on the kinetics of human immunodeficiency virus type 1 vector transduction
    Bénédicte Van Maele
    Rega Institute for Medical Research, Leuven, Flanders, Belgium
    J Virol 77:4685-94. 2003
    ....
  91. ncbi p38 is a key signaling molecule for H-ras-induced inhibition of gap junction intercellular communication in rat liver epithelial cells
    Ki Won Lee
    Department of Food Science and Technology, School of Agricultural Biotechnology, Seoul National University, Seoul 151 742, South Korea
    Ann N Y Acad Sci 1030:258-63. 2004
    ..Thus, p38 is a key signaling molecule for H-ras-induced inhibition of GJIC in WB cells...
  92. pmc Various members of the Toll-like receptor family contribute to the innate immune response of human epidermal keratinocytes
    Gabriele Köllisch
    Division of Environmental Dermatology and Allergy GSF TUM, GSF National Research Center for Environment and Health, Neuherberg, Germany
    Immunology 114:531-41. 2005
    ..These studies provide substantial evidence for a functional TLR expression and signalling profile of normal human keratinocytes contributing to the antimicrobial defence barrier of human skin...
  93. ncbi A dynamic podosome-like structure of epithelial cells
    Laura Spinardi
    DIBIT, Department of Biological and Technological Research, San Raffaele Scientific Institute and University Vita Salute San Raffaele, 20132 Milan, Italy
    Exp Cell Res 295:360-74. 2004
    ..We show that podosome-like structures are not unique to cells of mesenchymal origin, but also appear in epithelial cells, where they seem to be related to basement membrane adhesion...
  94. pmc The best disease-linked Cl- channel hBest1 regulates Ca V 1 (L-type) Ca2+ channels via src-homology-binding domains
    Kuai Yu
    Department of Cell Biology, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    J Neurosci 28:5660-70. 2008
    ..Our findings reveal a novel function of bestrophin in regulation of Ca(V) channels and suggest a possible mechanism for the role of hBest1 in macular degeneration...
  95. pmc Mechanisms regulating plasminogen activators in transformed retinal ganglion cells
    Nathan Rock
    Eye Research Institute, Oakland University, Rochester, MI 48309, USA
    Exp Eye Res 86:492-9. 2008
    ..These results indicate that LRP-1 regulates autocrine synthesis of tPA and uPA in RGC-5 cells and suggest that the use of RAP to antagonize the effect of proteases may be a way to prevent RGC death in glaucoma...
  96. pmc The oncoprotein H-RasV12 increases mitochondrial metabolism
    Sucheta Telang
    Molecular Targets Group, Department of Medicine, James Graham Brown Cancer Center, University of Louisville, Louisville, Kentucky 40202, USA
    Mol Cancer 6:77. 2007
    ....
  97. ncbi Human podocytes possess a stretch-sensitive, Ca2+-activated K+ channel: potential implications for the control of glomerular filtration
    Michael J Morton
    School of Biomedical Sciences, Worsley Building, University of Leeds, Leeds LS2 9NQ, UK
    J Am Soc Nephrol 15:2981-7. 2004
    ..By implication, these results suggest that podocytes may be able to respond to changes in the glomerular capillary pressure and modulate the GFR...
  98. ncbi Site-directed mutagenesis of the ATM promoter: consequences for response to proliferation and ionizing radiation
    Nuri Gueven
    Queensland Cancer Fund Research Laboratory, The Queensland Institute of Medical Research, Royal Brisbane Hospital, Brisbane, Queensland, Australia
    Genes Chromosomes Cancer 38:157-67. 2003
    ..The data described here provide additional evidence that basal and radiation-induced regulation of the ATM promoter is under multifactorial control...
  99. ncbi Coenzyme Q10 protects retinal cells against oxidative stress in vitro and in vivo
    Yoshimi Nakajima
    Department of Biofunctional Evaluation, Molecular Pharmacology, Gifu Pharmaceutical University, 5 6 1 Mitahora Higashi, Gifu 502 8585, Japan
    Brain Res 1226:226-33. 2008
    ..To investigate the neuroprotective effects of coenzyme Q10 and/or a vitamin E analogue on retinal damage both in vitro and in vivo...
  100. ncbi Telomere maintenance requires the RAD51D recombination/repair protein
    Madalena Tarsounas
    Cancer Research UK, London Research Institute, Clare Hall Laboratories, Blanche Lane, South Mimms, Hertfordshire, EN6 3LD, United Kingdom
    Cell 117:337-47. 2004
    ..We conclude that RAD51D plays a dual cellular role in both the repair of DNA double-strand breaks and telomere protection against attrition and fusion...
  101. pmc Human telomerase reverse transcriptase (hTERT) Q169 is essential for telomerase function in vitro and in vivo
    Haley D M Wyatt
    Southern Alberta Cancer Research Institute and Department of Biochemistry and Molecular Biology, University of Calgary, Calgary, Alberta, Canada
    PLoS ONE 4:e7176. 2009
    ..A conserved glutamine residue in the TERT N-terminus is important for anchor site interactions in lower eukaryotes. The significance of this residue in higher eukaryotes, however, has not been investigated...

Research Grants62

  1. REGULATION OF TISSUE FACTOR PATHWAY INHIBITOR EXPRESSION
    MADHU BAJAJ; Fiscal Year: 2000
    ..In gel mobility shift assays, factor(s) from nuclear extracts of HepG2 cells (a transformed cell line that expresses both TFPI and GATA-2 transcription factor) appear to bind to a DNA fragment from the TFPI ..
  2. RELATIONSHIPS BETWEEN PROSTANOIDS AND CELLULAR INJURY
    John Flynn; Fiscal Year: 2000
    ..The initial aim of the proposal is to fully characterize this transformed cell line and to compare its response to primary cultures of MECs with regard to LPS responsiveness and other ..
  3. CELL BIOLOGY OF PHOSPHATIDYLCHOLINE
    Susan Jaken; Fiscal Year: 1990
    ..6 nmol/mg protein/hr). These results indicate that the lack of responsiveness in the transformed cell line is not due to loss of the enzyme, but may be due to either a lesion in the phorbol ester receptor/protein ..
  4. HIV1 INHIBITORY PROTEINS FROM TRANSFORMED CD8+ CELLS
    Mary Klotman; Fiscal Year: 2002
    ..and potent soluble factor by completing the cloning of the single 8kd protein isolated from a CD8+ HVS- transformed cell line, expression and purification of the inhibitory protein in a bacterial expression system, determination of ..
  5. Anti-HPV RNA Interference Using Modified RNA's
    Kenneth Alexander; Fiscal Year: 2005
    ..Similar results have been obtained in SW756 cells (a second HPV-18-transformed cell line), but not in C33A cells (a non-HPV-transformed cervical carcinoma cell line)...
  6. THE FATE OF CYTOCHROME C IN CELL DEATH
    Ronald Jemmerson; Fiscal Year: 2000
    ..c which may be relevant for B cell recognition has been isolated from the culture fluid of an apoptotic transformed cell line. Additional studies of a variety of cell types will be carried out to identify similarities and ..
  7. RESPONSE OF NEURONS TO ELECTRICAL FIELDS
    Kenneth Robinson; Fiscal Year: 1992
    ..As there may be some doubts about using a transformed cell line as a model for normal neuronal development or regeneration, some primary cultures of disaggregated neurons ..
  8. MECHANISM OF MOUSE LUNG CARCINOGENESIS
    Ming You; Fiscal Year: 2006
    ..In addition, wild type K-ras allele inhibited colony formation and tumor development of a transformed cell line that contained a mutant K-ras...
  9. MECHANISMS OF REGULATION OF CELL PROLIFERATION
    Michael Getz; Fiscal Year: 1993
    ..mechanisms are responsible for altered patterns of beta-actin expression in at least one chemically-transformed cell line. These and alternate possibilities will be tested by delineating cis-acting regulatory elements and their ..
  10. PURIFICATION AND CHARACTERIZATION OF A TNF INHIBITOR
    Susan Wright; Fiscal Year: 1990
    ..Culture supernatants harvested from a transformed cell line will be subjected to standard protein purification procedures to accomplish this goal...
  11. Protein Kinase G Regulation of Granulosa Cell Viability
    John Peluso; Fiscal Year: 2006
    ..We now propose to test this hypothesis using biochemical, genetic, imaging and cell biological techniques. [unreadable] [unreadable]..
  12. Progesterone regulation of human luteal cell viability
    John Peluso; Fiscal Year: 2006
    ..Such selective manipulation of the P4's actions could have far reaching effects on the treatment for infertility, contraception and certain types of cancers. [unreadable] [unreadable]..
  13. Regulation of Oocyte Viability by Granulosa Cell Contact
    John Peluso; Fiscal Year: 2004
    ..abstract_text> ..
  14. A thyroid receptor co-activator hypothesis for psychosis
    Robert Philibert; Fiscal Year: 2005
    ..The net effect will be to produce an independent investigator capable of functional and translational research. ..
  15. Genetic Studies of Substance Abuse in Iowa Adoptees
    Robert Philibert; Fiscal Year: 2008
    ..unreadable] [unreadable]..
  16. HUMAN MAMMARY CELLS
    Martha Stampfer; Fiscal Year: 2002
    ..e. antisense telomerase RNA) activity. Additionally, the principal investigator will continue to collaborate with other investigators who wish to work with her cell lines. ..
  17. P2Y2R MEDIATED IMMUNE RESPONSES IN SALIVARY GLAND DYSFUNCTION
    Olga Baker; Fiscal Year: 2008
    ..These studies may lead to better therapeutic strategies for minimizing autoimmune-associated dysfunction of salivary gland that contributes to xerostomia in patients with SS. ..
  18. Amnis ImageStream Flow Cytometer
    Craig Jordan; Fiscal Year: 2007
    ..Taken together, the unique capabilities of the ImageStream system will therefore serve to strongly benefit a wide range of research activities. [unreadable] [unreadable] [unreadable]..
  19. Lymphocyte Survival: Implications for lymphomagenesis
    Craig B Thompson; Fiscal Year: 2010
    ....
  20. Role of PP2A in tauopathies
    Estelle Sontag; Fiscal Year: 2006
    ....
  21. Dynamic Analysis of Rho GTPase-Rho GDI Cycling
    Leslie M Loew; Fiscal Year: 2010
    ..With our studies, we hope to provide a quantitatively detailed, predictive model that can be used to investigate Rho GTPase function in health and disease. ..
  22. Transcriptional control of FSH expression
    Daniel Bernard; Fiscal Year: 2007
    ..Given the critical importance of FSH for fertility in women, these results may aid in the design of novel contraceptives or may pinpoint causes of infertility that have thus far eluded our detection. ..
  23. HIV protease inhibitors, marcophage function/estrogen
    Melinda Wilson; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  24. Transport of neurotrophic cytokines after spinal cord injury
    Weihong Pan; Fiscal Year: 2007
    ..Understanding the mechanisms of cytokine transport at this regulatory interface would help in the design of new approaches to treat SCI. ..
  25. T Regulatory Cells and Childhood Asthma
    James Gern; Fiscal Year: 2008
    ..abstract_text> ..
  26. Skin Manifestations of Tuberous Sclerosis
    Jack Arbiser; Fiscal Year: 2009
    ..Specific Aim 3. To determine whether dysregulation of p16ink4a and PTCH (patched) acts as a modifier of theTS phenotype in transgenic mice expressing dominant negative tuberin. ..
  27. Oncogenic properties of interferon regulatory factor 7
    Luwen Zhang; Fiscal Year: 2009
    ..This study should provide an insight of how IRF-7 participates in EBV transformation, and expand our knowledge about IRFs as well as the pathogenesis of EBV-associated CNS lymphoma. ..
  28. Genetic Analysis of Papillomavirus Virion Morphogenesis
    Craig Meyers; Fiscal Year: 2009
    ....
  29. Vaccinia: T-cell immunity and homing to skin
    David Koelle; Fiscal Year: 2009
    ..Our data will assist in evaluating the immunogenicity of these constructs. ..
  30. Role of Cell Mediators in Asbestos-SV40 Carcinogenesis
    Michele Carbone; Fiscal Year: 2009
    ..It is anticipated that the results of the experimens proposed in this application will provide information to design novel preventive and therapeutic approaches for MM. ..
  31. ABERRANT RETINOID SIGNALING IN BREAST CANCER
    Eduardo F Farias; Fiscal Year: 2010
    ..This has direct implications to future retinoid clinical trials in that it advocates that participating patients be carefully stratified based on the molecular characteristics of their cancer. ..
  32. SHIP-2 influence on macrophage Fc receptor function
    Susheela Tridandapani; Fiscal Year: 2009
    ..The proposed work holds promise for a better understanding of the IC-mediated inflammatory diseases at the molecular level, and paves the way for novel therapeutic targets. ..
  33. A FADD-Associated Helicase Essential for Host Defense
    Glen Barber; Fiscal Year: 2008
    ..We anticipate that these studies will improve our knowledge of pathogenesis and provide new concepts for the rational design of novel therapeutic and vaccine strategies. [unreadable] [unreadable] [unreadable]..
  34. MOLECULAR MECHANISMS IN NEOPLASIA
    Paul Neiman; Fiscal Year: 2008
    ..In this fashion we hope to establish whether Myc-induced genomic instability merits wider exploration in human neoplasm. [unreadable] [unreadable] [unreadable] [unreadable]..
  35. Novel Therapeutic Agents for Diabetic Ulcers
    JUNG HUANG; Fiscal Year: 2008
    ..The novel antagonists developed in this project should be ideal drug candidates for treating diabetic foot ulcers in humans. [unreadable] [unreadable] [unreadable]..
  36. NQO1 Inhibitors and Pancreatic Cancer Therapy
    David Ross; Fiscal Year: 2008
    ..This approach represents a novel therapeutic strategy for the treatment of pancreatic cancer, a disease where therapeutic options are very limited and where chemotherapy has made minimal impact. ..
  37. Molecular and Cellular Mechanisms of Glaucoma
    OLEG KISSELEV; Fiscal Year: 2007
    ..abstract_text> ..
  38. Ion Regulation of Kv Channel Gating and Permeation
    Carol Deutsch; Fiscal Year: 2007
    ..The second aim will test whether ions in the selectivity filter modulate the movement of voltage sensors. These studies will entail measurements of kinetics and voltage dependence of gating currents in HEK cells. ..
  39. Defining an HIV Integrase Inhibitor Binding Pocket
    William Robinson; Fiscal Year: 2004
    ..Moreover, by understanding the nature of inhibitor resistance, new molecules with activity against inhibitor-resistant HIV can be designed and synthesized. ..
  40. IBD--MUCOSA-SPECIFIC REGULATION OF IFN-GAMMA PRODUCTION
    Stephan Targan; Fiscal Year: 2005
    ..abstract_text> ..
  41. Activin signaling mechanisms in pituitary cells
    Daniel Bernard; Fiscal Year: 2005
    ..The results of the proposed studies will increase our understanding of mechanisms of FSH regulation by activins, may highlight novel targets for contraceptive design, and may provide insights into causes of some forms of infertility. ..
  42. Identification of a new protein that binds Src and PKC
    STEPHEN SOLTOFF; Fiscal Year: 2004
    ..Future efforts will determine if this new protein alters cell morphology, tight junction formation, or other functions in salivary cells. ..
  43. TRANSGENIC STUDIES OF AMYOTROPHIC LATERAL SCLEROSIS
    Han Xiang Deng; Fiscal Year: 2004
    ..To achieve this goal we propose to develop additional transgenic mouse lines that over express successively smaller fragments of SOD 1 polypeptide to determine the smallest segment of SOD 1 that causes ALS. ..
  44. LIVER/LUNG INTERACTIONS DURING GRAM NEGATIVE ENDOTOXEMIA
    George Matuschak; Fiscal Year: 2003
    ....
  45. MOLECULAR BASIS OF ORGANELLE MOVEMENT IN AXONS
    Michael Sheetz; Fiscal Year: 2002
    ....
  46. DIFFERENTIAL MODULATION OF GABAERGIC SYNAPSES BY KARS
    Jian Kang; Fiscal Year: 2005
    ..The KAR-mediated modulation of GABAergic synapses provides a fundamental new mechanism for glutamate-mediated interaction between excitatory and inhibitory synapses. ..
  47. Action of Novel Anti-tumor Agents on Cell Communication
    Diane Matesic; Fiscal Year: 2005
    ..abstract_text> ..
  48. JUN KINASE AND HUMAN TUMORS
    Dan Mercola; Fiscal Year: 2001
    ....
  49. Development and Testing of a Novel Neuroprotective Drug
    David Schubert; Fiscal Year: 2006
    ..The results from these studies will determine the therapeutic potential of this novel compound. [unreadable] [unreadable]..
  50. EIAV vector targeting dendritic cells to induce CTL
    ROBERT MEALEY; Fiscal Year: 2005
    ..Thus, this proposal will test the hypothesis that targeting dendritic cells in vivo with an EIAV vector will induce CTL in horses to multiple viral proteins. ..
  51. Integrated Methods to Discover Novel Disease Pathways
    Richard Del Mastro; Fiscal Year: 2005
    ..abstract_text> ..
  52. AADRC -- Mechanisms of Virus-Induced Asthma
    James Gern; Fiscal Year: 2005
    ..It is proposed that these collaborative studies will provide new insight into mechanisms of respiratory-virus-induced asthma, the possible role of diminished IFN-gamma production in this process, and possibilities for new treatment...
  53. 2006 Gordon Research Conference on Signal Transduction By Engineered ECM
    Michael Sheetz; Fiscal Year: 2006
    ..A special effort will be made to use the financial support requested to facilitate the attendance and participation of women and minorities in this meeting. [unreadable] [unreadable] [unreadable]..
  54. Epithelial Na channel (ENaC) polymorphisms in hyptertention
    James Stockand; Fiscal Year: 2007
    ..Preliminary results support the feasibility of this investigation and suggest that this line of inquiry will yield significant and novel findings. [unreadable] [unreadable]..
  55. Redox Sensitive Proteins in Aging and Neurodegeneration
    David Schubert; Fiscal Year: 2006
    ..These proteins, in turn, will be used as targets for the identification of potentially therapeutic antioxidants that could be useful in preventing age-and disease-associated damage to the CNS. ..
  56. Single Fiber Forces from Cardiac Fibroblasts
    Michael Sheetz; Fiscal Year: 2004
    ..abstract_text> ..
  57. Selective in vivo Ablation of Leukemia Stem Cells
    Craig Jordan; Fiscal Year: 2009
    ..Collectively, the proposed studies will provide a detailed evaluation of how therapeutic agents impact the growth and survival of LSC in vivo. ..
  58. Mechanisms of HIV Transport Across the BBB
    William Banks; Fiscal Year: 2004
    ....
  59. SELENIUM AND LUNG CANCER RISK
    Henry Thompson; Fiscal Year: 2004
    ..In addition, this work has the potential to provide new biomarkers with which to assess lung cancer risk and its modulation. ..
  60. Towards Developing type 1a HCV cell culture model
    Asim Dasgupta; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  61. CHARACTERIZATION OF EARLY STAGE PROSTATE CANCER
    Dan Mercola; Fiscal Year: 2004
    ..Discovering molecular profiles within prostate tumors that prognosticate for patient outcome will greatly improve the quality of life for these patients. ..