transformed cell line

Summary

Summary: Eukaryotic cell line obtained in a quiescent or stationary phase which undergoes conversion to a state of unregulated growth in culture, resembling an in vitro tumor. It occurs spontaneously or through interaction with viruses, oncogenes, radiation, or drugs/chemicals.

Top Publications

  1. pmc HTLV-I basic leucine zipper factor gene mRNA supports proliferation of adult T cell leukemia cells
    Yorifumi Satou
    Laboratory of Virus Immunology, Institute for Virus Research, Kyoto University, 53 Shogoin Kawahara cho, Sakyo ku, Kyoto 606 8507, Japan
    Proc Natl Acad Sci U S A 103:720-5. 2006
  2. ncbi The Bloom's syndrome gene product interacts with topoisomerase III
    L Wu
    Imperial Cancer Research Fund Laboratories, Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford OX3 9DS, United Kingdom
    J Biol Chem 275:9636-44. 2000
  3. ncbi Gliotoxin-mediated apoptosis of activated human hepatic stellate cells
    Young Oh Kweon
    Division of Digestive Diseases and Nutrition, Department of Medicine, CB 7038, Glaxo Research Bldg Rm 156, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    J Hepatol 39:38-46. 2003
  4. pmc Three functional variants of IFN regulatory factor 5 (IRF5) define risk and protective haplotypes for human lupus
    Robert R Graham
    Program in Medical and Population Genetics, Broad Institute of Harvard and Massachusetts Institute of Technology, Cambridge, MA 02142, USA
    Proc Natl Acad Sci U S A 104:6758-63. 2007
  5. ncbi Association of gp91phox homolog Nox1 with anchorage-independent growth and MAP kinase-activation of transformed human keratinocytes
    Walee Chamulitrat
    Deutsches Krebsforschungszentrum, Department of Applied Tumorvirology, 69120 Heidelberg, Germany
    Oncogene 22:6045-53. 2003
  6. pmc Epstein-barr virus immediate-early protein BZLF1 is SUMO-1 modified and disrupts promyelocytic leukemia bodies
    A L Adamson
    Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 7295, USA
    J Virol 75:2388-99. 2001
  7. ncbi Reversal of senescence in mouse fibroblasts through lentiviral suppression of p53
    Annette M G Dirac
    Division of Molecular Carcinogenesis, and Center for Biomedical Genetics, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    J Biol Chem 278:11731-4. 2003
  8. pmc Mutations in the phosphatidylinositol-3-kinase pathway predict for antitumor activity of the inhibitor PX-866 whereas oncogenic Ras is a dominant predictor for resistance
    Nathan T Ihle
    M D Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, TX 77030, USA
    Cancer Res 69:143-50. 2009
  9. pmc Measurements of the BKCa channel's high-affinity Ca2+ binding constants: effects of membrane voltage
    Tara Beth Sweet
    Molecular Cardiology Research Institute, Tufts Medical Center, Tufts University School of Medicine, Boston, MA 02111, USA
    J Gen Physiol 132:491-505. 2008
  10. ncbi Mitochondrial repair of 8-oxoguanine is deficient in Cockayne syndrome group B
    Tinna Stevnsner
    Danish Center for Molecular Gerontology, Department of Molecular Biology, University of Aarhus, DK 8000 Aarhus C, Denmark
    Oncogene 21:8675-82. 2002

Detail Information

Publications227 found, 100 shown here

  1. pmc HTLV-I basic leucine zipper factor gene mRNA supports proliferation of adult T cell leukemia cells
    Yorifumi Satou
    Laboratory of Virus Immunology, Institute for Virus Research, Kyoto University, 53 Shogoin Kawahara cho, Sakyo ku, Kyoto 606 8507, Japan
    Proc Natl Acad Sci U S A 103:720-5. 2006
    ..Thus, the single HBZ gene has bimodal functions in two different molecular forms. The growth-promoting activity of HBZ RNA likely plays an important role in oncogenesis by HTLV-I...
  2. ncbi The Bloom's syndrome gene product interacts with topoisomerase III
    L Wu
    Imperial Cancer Research Fund Laboratories, Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford OX3 9DS, United Kingdom
    J Biol Chem 275:9636-44. 2000
    ..Furthermore, through characterizing a genetic interaction between BLM and TOP3 in S. cerevisiae, we have identified a functional role for the hTOPO IIIalpha interaction domains in BLM...
  3. ncbi Gliotoxin-mediated apoptosis of activated human hepatic stellate cells
    Young Oh Kweon
    Division of Digestive Diseases and Nutrition, Department of Medicine, CB 7038, Glaxo Research Bldg Rm 156, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    J Hepatol 39:38-46. 2003
    ..Gliotoxin induces apoptosis of activated human and rat HSCs by an unknown mechanism...
  4. pmc Three functional variants of IFN regulatory factor 5 (IRF5) define risk and protective haplotypes for human lupus
    Robert R Graham
    Program in Medical and Population Genetics, Broad Institute of Harvard and Massachusetts Institute of Technology, Cambridge, MA 02142, USA
    Proc Natl Acad Sci U S A 104:6758-63. 2007
    ....
  5. ncbi Association of gp91phox homolog Nox1 with anchorage-independent growth and MAP kinase-activation of transformed human keratinocytes
    Walee Chamulitrat
    Deutsches Krebsforschungszentrum, Department of Applied Tumorvirology, 69120 Heidelberg, Germany
    Oncogene 22:6045-53. 2003
    ..Furthermore, JNK2 was constitutively phosphorylated in FIB cells. Together, our data strongly implicate Nox1 in redox-mediated signaling related to cellular activation of human keratinocytes at a more advanced stage of transformation...
  6. pmc Epstein-barr virus immediate-early protein BZLF1 is SUMO-1 modified and disrupts promyelocytic leukemia bodies
    A L Adamson
    Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 7295, USA
    J Virol 75:2388-99. 2001
    ..These results suggest that disruption of PML bodies is important for efficient lytic replication of EBV. Furthermore, Z may potentially alter the function of a variety of cellular proteins by inhibiting SUMO-1 modification...
  7. ncbi Reversal of senescence in mouse fibroblasts through lentiviral suppression of p53
    Annette M G Dirac
    Division of Molecular Carcinogenesis, and Center for Biomedical Genetics, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    J Biol Chem 278:11731-4. 2003
    ..These data indicate that senescence in MEFs is reversible and demonstrate that both initiation and maintenance of senescence is p53-dependent...
  8. pmc Mutations in the phosphatidylinositol-3-kinase pathway predict for antitumor activity of the inhibitor PX-866 whereas oncogenic Ras is a dominant predictor for resistance
    Nathan T Ihle
    M D Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, TX 77030, USA
    Cancer Res 69:143-50. 2009
    ....
  9. pmc Measurements of the BKCa channel's high-affinity Ca2+ binding constants: effects of membrane voltage
    Tara Beth Sweet
    Molecular Cardiology Research Institute, Tufts Medical Center, Tufts University School of Medicine, Boston, MA 02111, USA
    J Gen Physiol 132:491-505. 2008
    ....
  10. ncbi Mitochondrial repair of 8-oxoguanine is deficient in Cockayne syndrome group B
    Tinna Stevnsner
    Danish Center for Molecular Gerontology, Department of Molecular Biology, University of Aarhus, DK 8000 Aarhus C, Denmark
    Oncogene 21:8675-82. 2002
    ..For the first time we report that CSB stimulates repair of 8-oxoG in mammalian mitochondrial DNA. Furthermore, evidence is presented to support the hypothesis that wild type CSB regulates expression of OGG1...
  11. ncbi In vivo recruitment of XPC to UV-induced cyclobutane pyrimidine dimers by the DDB2 gene product
    Maureen E Fitch
    Departments of Medicine and Genetics, Division of Oncology, Stanford University School of Medicine, 269 Campus Drive, Stanford, CA 94305, USA
    J Biol Chem 278:46906-10. 2003
    ..XPC did localize to CPDs when p48 was overexpressed in the same cell, signifying that p48 activates the recruitment of XPC to CPDs and may be the initial recognition factor in the NER pathway...
  12. ncbi Catalytically active human telomerase mutants with allele-specific biological properties
    Moses Kim
    Department of Biochemistry and Biophysics, University of California at San Francisco, San Francisco, CA 94143 2200, USA
    Exp Cell Res 288:277-87. 2003
    ..These results uncover further hTERT allele-specific phenotypes that uncouple telomerase activity, net telomere lengthening and life span extension...
  13. ncbi PAK4 functions in tumor necrosis factor (TNF) alpha-induced survival pathways by facilitating TRADD binding to the TNF receptor
    Xiaofan Li
    Department of Biological Sciences, Columbia University, New York, New York 10027, USA
    J Biol Chem 280:41192-200. 2005
    ..The role for PAK4 in regulating prosurvival pathways is a completely new function for this protein, and the connection between PAK4 and cell survival under stress helps explain its role in tumorigenesis and development...
  14. ncbi Requirement of mammalian DNA polymerase-beta in base-excision repair
    R W Sobol
    Sealy Center for Molecular Science, University of Texas Medical Branch, Galveston 77555 1068, USA
    Nature 379:183-6. 1996
    ..These studies demonstrate that beta-polymerase functions specifically in base-excision repair in vivo...
  15. ncbi ATM and DNA-PK function redundantly to phosphorylate H2AX after exposure to ionizing radiation
    Tom Stiff
    Genome Damage and Stability Centre, University of Sussex, East Sussex, United Kingdom
    Cancer Res 64:2390-6. 2004
    ..However, by phosphorylating H2AX, DNA-PK can contribute to the presence of the damage response proteins MDC1 and 53BP1 at the site of the DSB...
  16. ncbi Altered apoptotic gene expression and acquired apoptotic resistance in cadmium-transformed human prostate epithelial cells
    William E Achanzar
    Inorganic Carcinogenesis Section, National Cancer Institute at National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA
    Prostate 52:236-44. 2002
    ..To investigate these mechanisms, we performed molecular comparisons between the cadmium-transformed prostate epithelial cell line CTPE and the nontumorigenic parental line RWPE-1...
  17. ncbi DDB accumulates at DNA damage sites immediately after UV irradiation and directly stimulates nucleotide excision repair
    Mitsuo Wakasugi
    Faculty of Pharmaceutical Sciences, Kanazawa University, Takara machi, Kanazawa 920 0934, Japan
    J Biol Chem 277:1637-40. 2002
    ..These results suggest that DDB can rapidly translocate to the damaged DNA sites independent of functional XPA and XPC proteins and directly enhance the excision reaction by core repair factors...
  18. pmc E2f3 is critical for normal cellular proliferation
    P O Humbert
    Center for Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139 USA
    Genes Dev 14:690-703. 2000
    ..We conclude that E2F3 is critical for the transcriptional activation of genes that control the rate of proliferation of both primary and tumor cells...
  19. ncbi Mlx, a new Max-like bHLHZip family member: the center stage of a novel transcription factors regulatory pathway?
    G Meroni
    Telethon Institute of Genetics and Medicine, Milan, Italy
    Oncogene 19:3266-77. 2000
    ..The possible role of Mlx in an emerging regulatory pathway and acting parallel to the Max driven network is discussed...
  20. ncbi Cloning and characterization of the 5'-flanking region of the human transcription factor Sp1 gene
    M Nicolas
    Department of Biochemistry and Molecular Biology, School of Pharmacy, University of Barcelona, 08028 Barcelona, Spain
    J Biol Chem 276:22126-32. 2001
    ..Sp1 and Sp3 proteins bind to the downstream GC box located in the proximal promoter of Sp1. Furthermore, we demonstrate that the Sp1 protein activates Sp1 transcription activity; thus the Sp1 gene is autoregulated...
  21. ncbi HIV-1 Gag protein associates with F-actin present in microfilaments
    O Rey
    Department of Pediatrics, School of Medicine, University of California at Los Angeles 90095, USA
    Virology 220:530-4. 1996
    ..In vivo and in vitro analyses of this interaction indicated that the unprocessed Gag polyprotein is capable of association with polymerized actin (F-actin). Binding of Gag to F-actin may be involved in the assembly or budding of HIV-1...
  22. pmc Production of high-titer recombinant adeno-associated virus vectors in the absence of helper adenovirus
    X Xiao
    Gene Therapy Center, Division of Pharmaceutics, University of North Carolina at Chapel Hill, 27599, USA
    J Virol 72:2224-32. 1998
    ....
  23. doi Cultivation of an immortalized human corneal endothelial cell population and two distinct clonal subpopulations on thermo-responsive carriers
    Thomas Götze
    Leibniz Institute of Polymer Research Dresden, Max Bergmann Center of Biomaterials, Hohe Strasse 6, 01069, Dresden, Germany
    Graefes Arch Clin Exp Ophthalmol 246:1575-83. 2008
    ..We sought to study HCEC sheet cultivation on such cell culture carriers under serum-free conditions as the next consequential step in developing methods for generation of corneal endothelial cell transplants...
  24. ncbi A conditionally immortalized human podocyte cell line demonstrating nephrin and podocin expression
    Moin A Saleem
    Children s Renal Unit and Academic Renal Unit, University of Bristol, Southmead Hospital, Bristol, UK
    J Am Soc Nephrol 13:630-8. 2002
    ..In conclusion, the development of this cell line provides a new tool in the study of podocyte biology, which will enable accurate assessment of the behavior of these complex cells in health and disease...
  25. ncbi UV-induced ubiquitylation of XPC protein mediated by UV-DDB-ubiquitin ligase complex
    Kaoru Sugasawa
    Cellular Physiology Laboratory, RIKEN Discovery Research Institute, Japan Science and Technology Agency, Wako, Saitama 351 0198, Japan
    Cell 121:387-400. 2005
    ..Our results strongly suggest that ubiquitylation plays a critical role in the transfer of the UV-induced lesion from UV-DDB to XPC...
  26. ncbi Allosteric inhibitors of Bcr-abl-dependent cell proliferation
    Francisco J Adrian
    Biological Chemistry Department, Genomics Institute of the Novartis Research Foundation, 10675 John Jay Hopkins Drive, San Diego, California 92121, USA
    Nat Chem Biol 2:95-102. 2006
    ..We propose that this new class of compounds inhibits Bcr-abl kinase activity through an allosteric non-ATP competitive mechanism...
  27. ncbi Akt deficiency impairs normal cell proliferation and suppresses oncogenesis in a p53-independent and mTORC1-dependent manner
    Jennifer E Skeen
    Department of Biochemistry and Molecular Genetics, University of Illinois, Chicago, Illinois 60607, USA
    Cancer Cell 10:269-80. 2006
    ..Surprisingly, upon mTORC1 hyperactivation, the reduction in Akt activity does not impair cell proliferation and susceptibility to oncogenic transformation; thus, Akt may mediate these processes exclusively via mTORC1...
  28. ncbi Identification of MIP-1 alpha/LD78 as a monocyte chemoattractant released by the HTLV-I-transformed cell line MT4
    R Bertini
    Laboratories of Biotechnology, Research Center Dompé S p A, L Aquila, Italy
    AIDS Res Hum Retroviruses 11:155-60. 1995
    It is known that the HTLV-I-transformed cell line MT4 releases chemotactic activity for monocytes spontaneously...
  29. ncbi Epstein-Barr virus-encoded latent membrane protein 1 (LMP1) induces the expression of the cellular microRNA miR-146a
    Natalie Motsch
    Institute of Virology, University of Saarland Medical School, Homburg, Germany
    RNA Biol 4:131-7. 2007
    ..It is possible that the induction of miR-146a plays a role in the induction or maintenance of EBV latency by modulating innate immune responses to the virus infected host cell...
  30. ncbi Conditionally immortalized human glomerular endothelial cells expressing fenestrations in response to VEGF
    S C Satchell
    Academic Renal Unit, University of Bristol, Southmead Hospital, Bristol, UK
    Kidney Int 69:1633-40. 2006
    ..They express fenestrations which are upregulated in response to VEGF. These cells are a unique resource for further study of GEnC and their roles in glomerular filtration, glomerular disease, and response to glomerular injury...
  31. pmc Identification of a novel element involved in regulation of the lytic switch BZLF1 gene promoter of Epstein-Barr virus
    R J Kraus
    McArdle Laboratory for Cancer Research, University of Wisconsin Medical School, Madison, Wisconsin 53706-1599, USA
    J Virol 75:867-77. 2001
    ..We conclude that ZV functions as an important regulatory element of the BZLF1 promoter, with ZVR likely playing important roles in the maintenance of latency and reactivation of EBV...
  32. ncbi Convergence of the fanconi anemia and ataxia telangiectasia signaling pathways
    Toshiyasu Taniguchi
    Department of Pediatric Oncology, Dana Farber Cancer Institute and Department of Pediatrics, Children s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Cell 109:459-72. 2002
    ..Biallelic disruption of FANCD2 results in both MMC and IR hypersensitivity...
  33. pmc Genomic alterations link Rho family of GTPases to the highly invasive phenotype of pancreas cancer
    Alec C Kimmelman
    Department of Medical Oncology, Dana Farber Cancer Institute and Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 105:19372-7. 2008
    ..Like RIOK3, PAK4 promotes pancreas ductal cell motility and invasion. Together, the genomic and functional profiles establish the Rho family GTP-binding proteins as integral to the hallmark invasive nature of this lethal disease...
  34. ncbi Inhibition of Egr-1 expression reverses transformation of prostate cancer cells in vitro and in vivo
    Veronique Baron
    Sidney Kimmel Cancer Center, 10835 Altman Row, San Diego, CA 92121, USA
    Oncogene 22:4194-204. 2003
    ..026). Thus, Egr-1 plays a functional role in the transformed phenotype and may represent a valid target for prostate cancer therapy...
  35. ncbi A mechanism in Epstein-Barr virus oncogenesis: inhibition of transforming growth factor-beta 1-mediated induction of MAPK/p21 by LMP1
    Makoto Fukuda
    Department of Biosignaling, School of Life Science, Faculty of Medicine, Tottori University, Yonago 683 8505, Japan
    Virology 302:310-20. 2002
    ....
  36. ncbi Formation of higher-order nuclear Rad51 structures is functionally linked to p21 expression and protection from DNA damage-induced apoptosis
    Elke Raderschall
    Max Planck Institute of Molecular Genetics, 14195 Berlin, Germany
    J Cell Sci 115:153-64. 2002
    ..This mechanism may contribute to a highly effective recombinational DNA repair in cell cycle-arrested cells and protection against DNA damage-induced apoptosis...
  37. pmc Recruitment of a protein complex containing Tat and cyclin T1 to TAR governs the species specificity of HIV-1 Tat
    P D Bieniasz
    Howard Hughes Medical Institute and Department of Genetics, Box 3025, Room 426, CARL Building, Duke University Medical Center, Research Drive, Durham, NC 27710, USA
    EMBO J 17:7056-65. 1998
    ....
  38. pmc Developmentally regulated recruitment of transcription factors and chromatin modification activities to chicken lysozyme cis-regulatory elements in vivo
    Pascal Lefevre
    Molecular Medicine Unit, University of Leeds, St James s University Hospital, Leeds LS9 7TF, United Kingdom
    Mol Cell Biol 23:4386-400. 2003
    ..This indicates that a partially accessible chromatin structure of lineage-specific genes is a hallmark of hematopoietic progenitor cells...
  39. ncbi The vimentin cytoskeleton regulates focal contact size and adhesion of endothelial cells subjected to shear stress
    Daisuke Tsuruta
    Department of Cell and Molecular Biology, Northwestern University Medical School, Morton 4 616, 303 E Chicago Avenue, Chicago, IL 60611, USA
    J Cell Sci 116:4977-84. 2003
    ..More dramatically, such cells showed decreased adhesion to the substratum. These data provide evidence that the vimentin cytoskeleton regulates focal contact size and helps stabilize cell-matrix adhesions in endothelial cells...
  40. pmc Hemidesmosome formation is initiated by the beta4 integrin subunit, requires complex formation of beta4 and HD1/plectin, and involves a direct interaction between beta4 and the bullous pemphigoid antigen 180
    R Q Schaapveld
    Division of Cell Biology, The Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands
    J Cell Biol 142:271-84. 1998
    ..Unfolding of the cytoplasmic domain may be part of a mechanism by which the interaction of beta4 with other hemidesmosomal components, e.g., BP180, is regulated...
  41. ncbi Nephrotic plasma alters slit diaphragm-dependent signaling and translocates nephrin, Podocin, and CD2 associated protein in cultured human podocytes
    Richard J M Coward
    Academic and Children s Renal Unit, Southmead Hospital, University of Bristol, Bristol, UK
    J Am Soc Nephrol 16:629-37. 2005
    ..This work demonstrates that nephrotic plasma seems to be deficient in factors that act via the podocyte SD complex, which are essential in maintaining its physiologic function...
  42. ncbi Extracellular matrix-associated serine protease inhibitors (Mr 33,000, 31,000, and 27,000) are single-gene products with differential glycosylation: cDNA cloning of the 33-kDa inhibitor reveals its identity to tissue factor pathway inhibitor-2
    C N Rao
    Department of Dermatology, Northwestern University School of Medicine, Chicago, Illinois 60611 3008, USA
    Arch Biochem Biophys 335:82-92. 1996
    ..None or very little of the inhibitors (0-2%) remained in a conditioned medium. Because they are primarily associated with ECM, the inhibitors may play a major role in ECM remodeling and turnover...
  43. pmc Relationship between contact inhibition and intranuclear S100C of normal human fibroblasts
    M Sakaguchi
    Department of Cell Biology, Institute of Molecular and Cellular Biology, Okayama University Medical School, Okayama 700 8558, Japan
    J Cell Biol 149:1193-206. 2000
    ..These data indicate the possible involvement of nuclear S100C in the contact inhibition of cell growth...
  44. ncbi DNA methyltransferase 3b contributes to oncogenic transformation induced by SV40T antigen and activated Ras
    Kenzo Soejima
    Department of Medical Oncology, Dana Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA
    Oncogene 22:4723-33. 2003
    ..Regardless of mechanism, our data indicate that DNMT3b plays an important role in transformation...
  45. pmc LPP, an actin cytoskeleton protein related to zyxin, harbors a nuclear export signal and transcriptional activation capacity
    M M Petit
    Laboratory for Molecular Oncology, Center for Human Genetics, University of Leuven and Flanders Interuniversity Institute for Biotechnology, B 3000 Leuven, Belgium
    Mol Biol Cell 11:117-29. 2000
    ..Altogether, these results show that the LPP protein has multifunctional domains and may serve as a scaffold upon which distinct protein complexes are assembled in the cytoplasm and in the nucleus...
  46. pmc Multiple centrosomes arise from tetraploidy checkpoint failure and mitotic centrosome clusters in p53 and RB pocket protein-compromised cells
    Franck Borel
    Institut de Biologie Structurale Jean Pierre Ebel, Commissariat à l Energie Atomique Centre National de la Recherche Scientifique, 41 rue Jules Horowitz, 38027 Grenoble Cedex 1, France
    Proc Natl Acad Sci U S A 99:9819-24. 2002
    ..Finally, we find that mouse embryo fibroblasts spontaneously enter tetraploid G(1), explaining the previous demonstration of centrosome amplification by p53 abrogation alone in these cells...
  47. ncbi Expression of gene rrg is associated with reversion of NIH 3T3 transformed by LTR-c-H-ras
    S Contente
    Department of Pathology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814 4799
    Science 249:796-8. 1990
    ..Stable transfection with sense constructs did not alter the normal morphology, message level, or nontumorigenicity of the persistent revertant cell line...
  48. ncbi The mechanisms controlling the recognition of tumor- and virus-infected cells by NKp46
    Tal I Arnon
    Lautenberg Center for General and Tumor Immunology, The Hebrew University Hadassah Medical School, Jerusalem, 91120, Israel
    Blood 103:664-72. 2004
    ..These results may explain the ability of NK cells to kill such a broad spectrum of viral-infected and tumor cells...
  49. ncbi Importance of poly(ADP-ribose) polymerase and its cleavage in apoptosis. Lesson from an uncleavable mutant
    F J Oliver
    UPR 9003 du Centre National de la Recherche Scientifique Cancérogenèse et Mutagenèse Moléculaire et Structurale, Laboratoire Conventionné, Illkirch Graffenstaden, France
    J Biol Chem 273:33533-9. 1998
    ....
  50. ncbi Transforming growth factor-beta1 promotes invasiveness after cellular transformation with activated Ras in intestinal epithelial cells
    K Fujimoto
    Department of Surgery, Vanderbilt University Medical Center and Vanderbilt-Ingram Cancer Center, Nashville, Tennessee 37232, USA
    Exp Cell Res 266:239-49. 2001
    ..The enhancement of invasiveness was correlated with decreased E-cadherin levels and subcellular distribution of beta-catenin. The enhancement of oncogenic Ras-mediated cell transformation by TGF-beta1 occurs via TbetaRII...
  51. ncbi Ras transformation requires metabolic control by 6-phosphofructo-2-kinase
    S Telang
    Molecular Targets Program, James Graham Brown Cancer Center, University of Louisville, Louisville, KY 40202, USA
    Oncogene 25:7225-34. 2006
    ....
  52. ncbi Clonal heterogeneity in differentiation potential of immortalized human mesenchymal stem cells
    Takeshi Okamoto
    Department of Tissue Regeneration, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara cho, Shogoin, Sakyo ku, Kyoto 606 8057, Japan
    Biochem Biophys Res Commun 295:354-61. 2002
    ..These cell lines, being the first established immortalized clonal cell lines of hMSCs, could provide insights into the mechanisms regulating the early steps of differentiation from undifferentiated MSCs into a specific lineage...
  53. pmc Impact of the central polypurine tract on the kinetics of human immunodeficiency virus type 1 vector transduction
    Bénédicte Van Maele
    Rega Institute for Medical Research, Leuven, Flanders, Belgium
    J Virol 77:4685-94. 2003
    ....
  54. pmc Various members of the Toll-like receptor family contribute to the innate immune response of human epidermal keratinocytes
    Gabriele Köllisch
    Division of Environmental Dermatology and Allergy GSF TUM, GSF National Research Center for Environment and Health, Neuherberg, Germany
    Immunology 114:531-41. 2005
    ..These studies provide substantial evidence for a functional TLR expression and signalling profile of normal human keratinocytes contributing to the antimicrobial defence barrier of human skin...
  55. ncbi p38 is a key signaling molecule for H-ras-induced inhibition of gap junction intercellular communication in rat liver epithelial cells
    Ki Won Lee
    Department of Food Science and Technology, School of Agricultural Biotechnology, Seoul National University, Seoul 151 742, South Korea
    Ann N Y Acad Sci 1030:258-63. 2004
    ..Thus, p38 is a key signaling molecule for H-ras-induced inhibition of GJIC in WB cells...
  56. ncbi A dynamic podosome-like structure of epithelial cells
    Laura Spinardi
    DIBIT, Department of Biological and Technological Research, San Raffaele Scientific Institute and University Vita Salute San Raffaele, 20132 Milan, Italy
    Exp Cell Res 295:360-74. 2004
    ..We show that podosome-like structures are not unique to cells of mesenchymal origin, but also appear in epithelial cells, where they seem to be related to basement membrane adhesion...
  57. pmc IAPs block apoptotic events induced by caspase-8 and cytochrome c by direct inhibition of distinct caspases
    Q L Deveraux
    The Burnham Institute, Program on Apoptosis and Cell Death Research, 10901 N Torrey Pines Road, La Jolla, CA 92037, USA
    EMBO J 17:2215-23. 1998
    ..These findings demonstrate that IAPs can suppress different apoptotic pathways by inhibiting distinct caspases and identify pro-caspase-9 as a new target for IAP-mediated inhibition of apoptosis...
  58. ncbi Human podocytes possess a stretch-sensitive, Ca2+-activated K+ channel: potential implications for the control of glomerular filtration
    Michael J Morton
    School of Biomedical Sciences, Worsley Building, University of Leeds, Leeds LS2 9NQ, UK
    J Am Soc Nephrol 15:2981-7. 2004
    ..By implication, these results suggest that podocytes may be able to respond to changes in the glomerular capillary pressure and modulate the GFR...
  59. ncbi Activation of a local tissue angiotensin system in podocytes by mechanical strain
    Raghu V Durvasula
    Department of Medicine, Division of Nephrology, University of Washington School of Medicine, Seattle, Washington 98195, USA
    Kidney Int 65:30-9. 2004
    ..Accordingly, we have tested the hypothesis that mechanical strain up-regulates local angiotensin II in podocytes, thereby resulting in a progressive reduction in podocyte number...
  60. ncbi Construction of a full-length human T cell leukemia virus type I genome from MT-2 cells containing multiple defective proviruses using overlapping polymerase chain reaction
    Takeo Ohsugi
    Division of Microbiology and Genetics, Center for Animal Resources and Development, Institute of Resource Development and Analysis, Kumamoto University, 2 2 1 Honjo, Kumamoto 860 0811, Japan
    Anal Biochem 329:281-8. 2004
    ..polymerase chain reaction (PCR) to construct a full-length provirus of HTLV-I directly from an HTLV-I-transformed cell line, MT-2, which contains multiple defective proviruses. First, four overlapping proviral HTLV-I fragments (1...
  61. ncbi The 33 kb transcript of the chicken alpha-globin gene domain is part of the nuclear matrix
    Sergey V Razin
    Institut J Monod, 2, place Jussieu, 75251 Paris, Cedex 05, France
    J Cell Biochem 92:445-57. 2004
    ..They may take part in the dynamic nuclear architecture when productively processed, or turn over slowly when globins are not synthesised...
  62. ncbi Telomere maintenance requires the RAD51D recombination/repair protein
    Madalena Tarsounas
    Cancer Research UK, London Research Institute, Clare Hall Laboratories, Blanche Lane, South Mimms, Hertfordshire, EN6 3LD, United Kingdom
    Cell 117:337-47. 2004
    ..We conclude that RAD51D plays a dual cellular role in both the repair of DNA double-strand breaks and telomere protection against attrition and fusion...
  63. pmc Lytic induction therapy for Epstein-Barr virus-positive B-cell lymphomas
    Wen hai Feng
    Department of Medicine, and Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    J Virol 78:1893-902. 2004
    ..These results suggest that the addition of GCV to either gemcitabine- or doxorubicin-containing chemotherapy regimens may enhance the therapeutic efficacy of these drugs for EBV-driven lymphoproliferative disease in patients...
  64. ncbi Dynamics of telomere erosion in transformed and non-transformed avian cells in vitro
    S E Swanberg
    Department of Animal Science, University of California, Davis, CA 95616, USA
    Cytogenet Genome Res 102:318-25. 2003
    ..Further, monitoring of telomere lengths of primary cells provides evidence for a dynamic series of changes over the lifespan of any specific cell culture ultimately resulting in net telomeric DNA loss by senescence...
  65. pmc EBNA-1, a bifunctional transcriptional activator
    Gregory Kennedy
    McArdle Laboratory for Cancer Research, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    Mol Cell Biol 23:6901-8. 2003
    ..EBNA-1's mode of activating transfected templates is therefore genetically distinct from that acting on integrated templates...
  66. ncbi Proactive transplantation of human neural stem cells prevents degeneration of striatal neurons in a rat model of Huntington disease
    Jae K Ryu
    Brain Disease Research Center, Ajou University School of Medicine, Suwon, South Korea
    Neurobiol Dis 16:68-77. 2004
    ..The findings in this work have particular relevance to a rat model of HD in that proactive transplanted hNSCs protect host striatal neurons against neuronal injury and improve motor impairment induced by 3-NP toxicity...
  67. ncbi Site-directed mutagenesis of the ATM promoter: consequences for response to proliferation and ionizing radiation
    Nuri Gueven
    Queensland Cancer Fund Research Laboratory, The Queensland Institute of Medical Research, Royal Brisbane Hospital, Brisbane, Queensland, Australia
    Genes Chromosomes Cancer 38:157-67. 2003
    ..The data described here provide additional evidence that basal and radiation-induced regulation of the ATM promoter is under multifactorial control...
  68. ncbi Spontaneously immortalized human T lymphocytes develop gain of chromosomal region 2p13-24 as an early and common genetic event
    Jan Konrad Siwicki
    Department of Immunology, Maria Skłodowska Curie Memorial Cancer Center and Institute of Oncology, Warsaw, Poland
    Genes Chromosomes Cancer 41:133-44. 2004
    ....
  69. ncbi Specific packaging of APOBEC3G into HIV-1 virions is mediated by the nucleocapsid domain of the gag polyprotein precursor
    Alexandra Schäfer
    Howard Hughes Medical Institute and Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC 27710, USA
    Virology 328:163-8. 2004
    ..Surprisingly, RNA was also found to be essential for formation of the nucleocapsid--APOBEC3G complex in vitro, thus raising the possibility that RNA may form a bridge between these two proteins...
  70. ncbi Reconstitution of telomerase activity combined with HPV-E7 expression allow human preadipocytes to preserve their differentiation capacity after immortalization
    C Darimont
    Nestle Research Center, PO Box 44, Vers chez les Blanc, 1000 Lausanne 26, Switzerland
    Cell Death Differ 10:1025-31. 2003
    ..These data demonstrate, for the first time, that coexpression of hTERT and HPV-E7 in human preadipocytes allows cells not only to display an indefinite life span but also to retain their capacity to differentiate...
  71. pmc Poly(ADP-ribosyl)ation accelerates DNA repair in a pathway dependent on Cockayne syndrome B protein
    Claudia Flohr
    Institute of Pharmacy, University of Mainz, 55099 Mainz, Germany
    Nucleic Acids Res 31:5332-7. 2003
    ..The results indicate that PARP activation and Csb are both involved in a novel mechanism that accelerates the global repair of various types of DNA modifications...
  72. ncbi Human neural stem cells genetically modified for brain repair in neurological disorders
    Seung U Kim
    Brain Disease Research Center, Ajou University School of Medicine, Suwon, Korea
    Neuropathology 24:159-71. 2004
    ..There is further need for investigation into the neurogenic potential of the human bone marrow stem cell lines and their utility in animal models of neurological diseases...
  73. ncbi Activation of the ATR-mediated DNA damage response by the HIV-1 viral protein R
    Mikhail Roshal
    Department of Microbiology and Immunology, University of Rochester Cancer Center, Rochester, New York 14642, USA
    J Biol Chem 278:25879-86. 2003
    ..These observations are important toward understanding how HIV infection promotes cell cycle disruption, cell death, and ultimately, CD4+ lymphocyte depletion...
  74. ncbi Matricellular protein SPARC is translocated to the nuclei of immortalized murine lens epithelial cells
    Qi Yan
    Hope Heart Program, Benaroya Research Institute at Virginia Mason, Seattle, Washington 98104 2046, USA
    J Cell Physiol 203:286-94. 2005
    ..SPARC therefore functions not only as an extracellular protein but also potentially as an intracellular protein to influence cellular activities and homeostasis...
  75. ncbi Deficiency of polycystin-2 reduces Ca2+ channel activity and cell proliferation in ADPKD lymphoblastoid cells
    Gianluca Aguiari
    Department of Biochemistry and Molecular Biology, University of Ferrara, Ferrara, Italy
    FASEB J 18:884-6. 2004
    ..Therefore, PC2 and PC1 are functionally expressed in LCLs, which provide a model, easily obtainable from ADPKD patients, to study PKD gene expression and function...
  76. pmc Evidence that the retroviral DNA integration process triggers an ATR-dependent DNA damage response
    Rene Daniel
    Institute for Cancer Research, Fox Chase Cancer Center, 7701 Burholme Avenue, Philadelphia, PA 19111, USA
    Proc Natl Acad Sci U S A 100:4778-83. 2003
    ..Components of the cellular DNA damage repair response may represent potential targets for antiretroviral drug development...
  77. pmc Iron and contact with host cells induce expression of adhesins on surface of Trichomonas vaginalis
    Ana F Garcia
    Department of Microbiology, University of Texas Health Science Center, San Antonio, TX, USA
    Mol Microbiol 47:1207-24. 2003
    ..Overall, the results confirm an important role for iron and contact in the surface expression of adhesins of T. vaginalis organisms...
  78. pmc v-Myb mediates cooperation of a cell-specific enhancer with the mim-1 promoter
    Olesya Chayka
    Institut fur Biochemie, Westfalische Wilhelms Universitat Munster, Wilhelm Klemm Strasse 2, D 48149 Munster, Germany
    Mol Cell Biol 25:499-511. 2005
    ....
  79. pmc Inhibition of plasminogen activators attenuates the death of differentiated retinal ganglion cells and stabilizes their neurite network in vitro
    Ronée Harvey
    Eye Research Institute of Oakland University, Rochester, MI 48309, USA
    Invest Ophthalmol Vis Sci 48:1884-91. 2007
    ....
  80. doi Coenzyme Q10 protects retinal cells against oxidative stress in vitro and in vivo
    Yoshimi Nakajima
    Department of Biofunctional Evaluation, Molecular Pharmacology, Gifu Pharmaceutical University, 5 6 1 Mitahora Higashi, Gifu 502 8585, Japan
    Brain Res 1226:226-33. 2008
    ..To investigate the neuroprotective effects of coenzyme Q10 and/or a vitamin E analogue on retinal damage both in vitro and in vivo...
  81. ncbi Titanium dioxide (P25) produces reactive oxygen species in immortalized brain microglia (BV2): implications for nanoparticle neurotoxicity
    Thomas C Long
    Department of Environmental Sciences and Engineering, School of Public Health, University of North Carolina, Chapel Hill, North Carolina 27599 7431, USA
    Environ Sci Technol 40:4346-52. 2006
    ..Cell viability was maintained at all test concentrations (2.5-120 ppm) over the 18 h exposure period. These data indicate that mouse microglia respond to Degussa P25 with cellular and morphological expressions of free radical formation...
  82. pmc The best disease-linked Cl- channel hBest1 regulates Ca V 1 (L-type) Ca2+ channels via src-homology-binding domains
    Kuai Yu
    Department of Cell Biology, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    J Neurosci 28:5660-70. 2008
    ..Our findings reveal a novel function of bestrophin in regulation of Ca(V) channels and suggest a possible mechanism for the role of hBest1 in macular degeneration...
  83. pmc Mechanisms regulating plasminogen activators in transformed retinal ganglion cells
    Nathan Rock
    Eye Research Institute, Oakland University, Rochester, MI 48309, USA
    Exp Eye Res 86:492-9. 2008
    ..These results indicate that LRP-1 regulates autocrine synthesis of tPA and uPA in RGC-5 cells and suggest that the use of RAP to antagonize the effect of proteases may be a way to prevent RGC death in glaucoma...
  84. pmc The oncoprotein H-RasV12 increases mitochondrial metabolism
    Sucheta Telang
    Molecular Targets Group, Department of Medicine, James Graham Brown Cancer Center, University of Louisville, Louisville, Kentucky 40202, USA
    Mol Cancer 6:77. 2007
    ....
  85. ncbi Establishment of immortal swine kidney epithelial cells
    Sungwook Kwak
    Laboratory of Cell Growth and Function Regulation, Division of Bioscience and Technology, College of Life and Environmental Sciences, Korea University, Seoul, Korea
    Anim Biotechnol 17:51-8. 2006
    ..Hence, three immortalized swine kidney epithelial cells that are not transformed would be valuable biological tools for virus propagation and basic kidney epithelial cell research...
  86. pmc Involvement of ER stress in retinal cell death
    Masamitsu Shimazawa
    Department of Biofunctional Molecules, Gifu Pharmaceutical University, Gifu, Japan
    Mol Vis 13:578-87. 2007
    ....
  87. pmc Replication protein A prevents accumulation of single-stranded telomeric DNA in cells that use alternative lengthening of telomeres
    Amra Grudic
    Section of Oncology, Department of Medicine, Haukeland University Hospital
    Nucleic Acids Res 35:7267-78. 2007
    ..This study demonstrates differences between ALT cells and telomerase-positive cells in the requirement for RPA in telomere processing and implicates the ALT mechanism in tumor cells as a possible therapeutic target...
  88. ncbi Oxidative stress is an early event in hydrostatic pressure induced retinal ganglion cell damage
    Quan Liu
    Hamilton Glaucoma Center and the Department of Ophthalmology, University of California San Diego, La Jolla, California 92037 0946, USA
    Invest Ophthalmol Vis Sci 48:4580-9. 2007
    ..To determine whether oxidative adduct formation or heme oxygenase-1 (HO-1) expression are altered in retinal ganglion cell (RGC) cultures exposed to elevated hydrostatic pressure and in a mouse model of glaucoma...
  89. ncbi Genome-wide SNP assay reveals structural genomic variation, extended homozygosity and cell-line induced alterations in normal individuals
    Javier Simon-Sanchez
    Molecular Genetics Unit, Departamento de Genómica y Proteómica, Instituto de Biomedicina de Valencia CSIC, 46010, Valencia, Spain
    Hum Mol Genet 16:1-14. 2007
    ..It is likely that this powerful methodology will augment existing techniques in the identification of chromosomal abnormalities...
  90. ncbi Mechanical strain increases SPARC levels in podocytes: implications for glomerulosclerosis
    Raghu V Durvasula
    Division of Nephrology, Box 356521, Univ of Washington School of Medicine, Seattle, WA 98195, USA
    Am J Physiol Renal Physiol 289:F577-84. 2005
    ..We speculate that the increase in SPARC may be maladaptive and lead to a progressive reduction in podocyte number, thus fueling the future development of glomerulosclerosis...
  91. ncbi Brain transplantation of immortalized human neural stem cells promotes functional recovery in mouse intracerebral hemorrhage stroke model
    Hong J Lee
    Division of Neurology, Department of Medicine, University of British Columbia Hospital, University of British Columbia, Vancouver, British Columbia V6T 2B5, Canada
    Stem Cells 25:1204-12. 2007
    ..Disclosure of potential conflicts of interest is found at the end of this article...
  92. pmc Human telomerase reverse transcriptase (hTERT) Q169 is essential for telomerase function in vitro and in vivo
    Haley D M Wyatt
    Southern Alberta Cancer Research Institute and Department of Biochemistry and Molecular Biology, University of Calgary, Calgary, Alberta, Canada
    PLoS ONE 4:e7176. 2009
    ..A conserved glutamine residue in the TERT N-terminus is important for anchor site interactions in lower eukaryotes. The significance of this residue in higher eukaryotes, however, has not been investigated...
  93. pmc Microarray reveals complement components are regulated in the serum-deprived rat retinal ganglion cell line
    Abdelnaby Khalyfa
    Department of Anatomical Sciences and Neurobiology, University of Louisville, School of Medicine, Louisville, KY 40292, USA
    Mol Vis 13:293-308. 2007
    ..The aim of this study was to generate a genome-wide gene expression of RGC-5 following serum deprivation and to identify candidate genes that may be involved in the signal transduction pathways...
  94. ncbi Spatio-temporal organization of DNA replication in murine embryonic stem, primary, and immortalized cells
    Margaret M Panning
    Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, 750 East Adams Street, Syracuse, New York 13210, USA
    J Cell Biochem 95:74-82. 2005
    ..These data suggest that heritable gene silencing during commitment to specific cell lineages is not mediated by global changes in the sub-nuclear organization and replication timing of chromosome domains...
  95. doi Competitive ELISA using a rabies glycoprotein-transformed cell line to semi-quantify rabies neutralizing-related antibodies in dogs
    Shoufeng Zhang
    Laboratory of Epidemiology, Veterinary Institute, Academy of Military Medical Sciences, 1068 Qinglong Road, Changchun 130062, PR China
    Vaccine 27:2108-13. 2009
    ..00 IU/ml. Since it can assay 40 or more samples in 1.5h, the c-ELISA is suitable for the surveillance of mass vaccination in dogs where rabies causes public concern...
  96. doi Genomic aberrations and cellular heterogeneity in SV40-immortalized human corneal epithelial cells
    Kenta Yamasaki
    Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kawaramachi dori, Kamigyo ku, Kyoto, Japan
    Invest Ophthalmol Vis Sci 50:604-13. 2009
    ..The nature of this cell line was assessed for genomic aberrations and cellular heterogeneity...
  97. ncbi Serum deprivation induces apoptotic cell death of transformed rat retinal ganglion cells via mitochondrial signaling pathways
    Irma Charles
    Department of Cell Biology and Genetics, University of North Texas Health Science Center, Fort Worth, Texas, USA
    Invest Ophthalmol Vis Sci 46:1330-8. 2005
    ..Apoptosis-related signaling pathways were investigated in a cultured rat retinal ganglion cell (RGC-5) line deprived of growth factors after serum withdrawal from the culture medium...
  98. ncbi The second loop of occludin is required for suppression of Raf1-induced tumor growth
    Zili Wang
    Epithelial Pathobiology Research Unit, Department of Pathology, Emory University, 615 Michael Street, Atlanta, GA 30322, USA
    Oncogene 24:4412-20. 2005
    ..These results demonstrate that the TJ protein occludin has a potent inhibitory effect on the Raf1-mediated tumorigenesis, and the second extracellular loop of occludin appears to be critical for this function...
  99. pmc MicroRNA-513 regulates B7-H1 translation and is involved in IFN-gamma-induced B7-H1 expression in cholangiocytes
    Ai Yu Gong
    Department of Medical Microbiology and Immunology, Creighton University Medical Center, Omaha, NE 68178, USA
    J Immunol 182:1325-33. 2009
    ..Thus, miR-513 regulates B7-H1 translation and is involved in IFN-gamma-induced B7-H1 expression in human cholangiocytes, suggesting a role for miRNA-mediated gene silencing in the regulation of cholangiocyte response to IFN-gamma...
  100. pmc Quantitative and functional analyses of spastin in the nervous system: implications for hereditary spastic paraplegia
    Joanna M Solowska
    Department of Neurobiology and Anatomy, Drexel University College of Medicine, Philadelphia, Pennsylvania 19129, USA
    J Neurosci 28:2147-57. 2008
    ..These results are consistent with a "gain-of-function" mechanism underlying HSP wherein spastin mutations produce a cytotoxic protein in the case of M1 but not M85...
  101. pmc Genotoxic stress and cellular stress alter the subcellular distribution of human T-cell leukemia virus type 1 tax through a CRM1-dependent mechanism
    Michael L Gatza
    Department of Molecular Virology and Microbiology, Baylor College of Medicine, One Baylor Plaza MS 385, Houston, TX 77030, USA
    J Virol 80:6657-68. 2006
    ..Changes in Tax distribution and interacting partners are likely to affect cellular processes that regulate cellular transformation...

Research Grants93

  1. NQO1 Inhibitors and Pancreatic Cancer Therapy
    David Ross; Fiscal Year: 2007
    ..This approach represents a novel therapeutic strategy for the treatment of pancreatic cancer, a disease where therapeutic options are very limited and where chemotherapy has made minimal impact. ..
  2. HMG-CoA reductase inhibitor, tea polyphenols and pancreatic cancer prevention
    Guang Yu Yang; Fiscal Year: 2007
    ..be performed using the selected immortalized human pancreatic ductal epithelial cell line and its k-ras transformed cell line in vitro...
  3. TUBULOINTERSTITIAL KIDNEY CELLS & REPONSE TO GLUCOSE
    Fuad Ziyadeh; Fiscal Year: 1993
    ..Preliminary data which we have gathered suggest that a transformed cell line derived from mouse cortical tubular epithelium represents an excellent model system for evaluating the ..
  4. HIV1 INHIBITORY PROTEINS FROM TRANSFORMED CD8+ CELLS
    Mary Klotman; Fiscal Year: 2002
    ..and potent soluble factor by completing the cloning of the single 8kd protein isolated from a CD8+ HVS- transformed cell line, expression and purification of the inhibitory protein in a bacterial expression system, determination of ..
  5. ION CHANNELS AND MONONUCLEAR PHAGOCYTE ACTIVATION
    Deborah Nelson; Fiscal Year: 2001
    ..and changes in intracellular pH in both primary human-derived macrophage cell lines and a macrophage-like transformed cell line. The goal will be to determine intervention points at which phagocytosis could be uncoupled from secretion...
  6. Anti-HPV RNA Interference Using Modified RNA's
    Kenneth Alexander; Fiscal Year: 2005
    ..Similar results have been obtained in SW756 cells (a second HPV-18-transformed cell line), but not in C33A cells (a non-HPV-transformed cervical carcinoma cell line)...
  7. MECHANISM OF MOUSE LUNG CARCINOGENESIS
    Ming You; Fiscal Year: 2006
    ..In addition, wild type K-ras allele inhibited colony formation and tumor development of a transformed cell line that contained a mutant K-ras...
  8. BIOCHEMISTRY OF ANIMAL VIRUS MULTIPLICATION
    Maurice Green; Fiscal Year: 1980
    ..HE C19 cells, an Ad12 transformed cell line that appears to have an intact late region will be used to investigate regulation...
  9. Regulation of Oocyte Viability by Granulosa Cell Contact
    John Peluso; Fiscal Year: 2004
    ..abstract_text> ..
  10. PAIRBP & PGRMC1 act as a membrane receptor complex to mediate P4's ovarian action
    John J Peluso; Fiscal Year: 2010
    ..These new pharmacological agents could have utility in the development contraceptives and in treating infertility and some forms of cancer. ..
  11. PAIRBP & PGRMC1 act as a membrane receptor complex to mediate P4's ovarian action
    John Peluso; Fiscal Year: 2007
    ..These new pharmacological agents could have utility in the development contraceptives and in treating infertility and some forms of cancer. ..
  12. PAIRBP & PGRMC1 act as a membrane receptor complex to mediate P4's ovarian action
    John Peluso; Fiscal Year: 2009
    ..These new pharmacological agents could have utility in the development contraceptives and in treating infertility and some forms of cancer. ..
  13. PAIRBP & PGRMC1 act as a membrane receptor complex to mediate P4's ovarian action
    John J Peluso; Fiscal Year: 2010
    ..These new pharmacological agents could have utility in the development contraceptives and in treating infertility and some forms of cancer. ..
  14. Protein Kinase G Regulation of Granulosa Cell Viability
    John Peluso; Fiscal Year: 2006
    ..We now propose to test this hypothesis using biochemical, genetic, imaging and cell biological techniques. ..
  15. Progesterone regulation of human luteal cell viability
    John Peluso; Fiscal Year: 2006
    ..Such selective manipulation of the P4's actions could have far reaching effects on the treatment for infertility, contraception and certain types of cancers. ..
  16. Genetic Studies of Substance Abuse in Iowa Adoptees
    Robert Philibert; Fiscal Year: 2007
    ....
  17. HUMAN MAMMARY CELLS
    Martha Stampfer; Fiscal Year: 2002
    ..e. antisense telomerase RNA) activity. Additionally, the principal investigator will continue to collaborate with other investigators who wish to work with her cell lines. ..
  18. METALLOPROTEINASE EXPRESSION IN CORNEAL WOUNDS
    Dimitri Azar; Fiscal Year: 2002
    ..The study of MMPs and angiostatin in the cornea may provide valuable information about their behavior and possible clinical significance in other tissue. ..
  19. CORE GRANT FOR VISION RESEARCH
    Dimitri Azar; Fiscal Year: 2007
    ..abstract_text> ..
  20. Novel Mechanisms of Quinone Toxicity
    David Ross; Fiscal Year: 2010
    ..The studies will have broad applicability to a variety of organ systems. ..
  21. Fluid Secretion in Parotid Cells: Signal Transduction
    STEPHEN SOLTOFF; Fiscal Year: 2007
    ..We suggest that these areas of investigation will provide a more complete understanding of parotid cell function, and thereby contribute to future therapies to aid salivary dysfunction. ..
  22. BIOCHEMICAL AND MOLECULAR STUDIES ON DT DIAPHORASE
    David Ross; Fiscal Year: 2005
    ..The proposed studies represent an integrated chemical, biochemical and molecular approach to determine the significance of NQO1 and NQO1-directed antitumor agents in chemotherapy. ..
  23. BIOCHEMICAL AND MOLECULAR STUDIES ON DT DIAPHORASE
    David Ross; Fiscal Year: 2007
    ..Our studies will generate data that can be applied to ongoing and future clinical studies of RH1 and both benzoquinone and hydroquinone ansamycins. ..
  24. Transport of TNFalpha across the BBB
    Weihong Pan; Fiscal Year: 2007
    ..These transporters could be novel drug targets and therefore provide promising therapeutic potential. ..
  25. Early cellular immune responses to EBV
    Sumita Bhaduri McIntosh; Fiscal Year: 2007
    ..It is also expected to enhance the understanding of EBV lymphoproliferative diseases and have potential diagnostic and therapeutic implications. ..