osteoblasts

Summary

Summary: Bone-forming cells which secrete an EXTRACELLULAR MATRIX. HYDROXYAPATITE crystals are then deposited into the matrix to form bone.

Top Publications

  1. ncbi LDL receptor-related protein 5 (LRP5) affects bone accrual and eye development
    Y Gong
    Cell 107:513-23. 2001
  2. ncbi Cell shape, cytoskeletal tension, and RhoA regulate stem cell lineage commitment
    Rowena McBeath
    The Cellular and Molecular Medicine Program, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Dev Cell 6:483-95. 2004
  3. ncbi Targeted disruption of Cbfa1 results in a complete lack of bone formation owing to maturational arrest of osteoblasts
    T Komori
    Department of Medicine III, Osaka University Medical School, Suita, Japan
    Cell 89:755-64. 1997
  4. ncbi The novel zinc finger-containing transcription factor osterix is required for osteoblast differentiation and bone formation
    Kazuhisa Nakashima
    Department of Molecular Genetics and Program in Genes and Development, M D Anderson Cancer Center, University of Texas, 1515 Holcombe Boulevard, Houston, TX 77030, USA
    Cell 108:17-29. 2002
  5. ncbi Osteoblastic cells regulate the haematopoietic stem cell niche
    L M Calvi
    Endocrine Unit, Department of Medicine, Center for Human Genetics and Molecular Pediatric Disease, University of Rochester School of Medicine, Rochester, New York 14642, USA
    Nature 425:841-6. 2003
  6. ncbi Osf2/Cbfa1: a transcriptional activator of osteoblast differentiation
    P Ducy
    Department of Molecular Genetics, M D Anderson Cancer Center, University of Texas, Houston 77030, USA
    Cell 89:747-54. 1997
  7. ncbi Wnt/beta-catenin signaling in mesenchymal progenitors controls osteoblast and chondrocyte differentiation during vertebrate skeletogenesis
    Timothy F Day
    Geneti Disease Research Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Dev Cell 8:739-50. 2005
  8. ncbi Identification of the haematopoietic stem cell niche and control of the niche size
    Jiwang Zhang
    Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA
    Nature 425:836-41. 2003
  9. ncbi Cbfa1, a candidate gene for cleidocranial dysplasia syndrome, is essential for osteoblast differentiation and bone development
    F Otto
    Imperial Cancer Research Fund, Lincoln s Inn Fields, London, United Kingdom
    Cell 89:765-71. 1997
  10. ncbi Canonical Wnt signaling in differentiated osteoblasts controls osteoclast differentiation
    Donald A Glass
    Department of Molecular and Human Genetics, Bone Disease Program of Texas, Houston, 77030, USA
    Dev Cell 8:751-64. 2005

Detail Information

Publications391 found, 100 shown here

  1. ncbi LDL receptor-related protein 5 (LRP5) affects bone accrual and eye development
    Y Gong
    Cell 107:513-23. 2001
    ..We demonstrate LRP5 expression by osteoblasts in situ and show that LRP5 can transduce Wnt signaling in vitro via the canonical pathway...
  2. ncbi Cell shape, cytoskeletal tension, and RhoA regulate stem cell lineage commitment
    Rowena McBeath
    The Cellular and Molecular Medicine Program, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Dev Cell 6:483-95. 2004
    ..These studies demonstrate that mechanical cues experienced in developmental and adult contexts, embodied by cell shape, cytoskeletal tension, and RhoA signaling, are integral to the commitment of stem cell fate...
  3. ncbi Targeted disruption of Cbfa1 results in a complete lack of bone formation owing to maturational arrest of osteoblasts
    T Komori
    Department of Medicine III, Osaka University Medical School, Suita, Japan
    Cell 89:755-64. 1997
    ..Examination of their skeletal systems showed a complete lack of ossification. Although immature osteoblasts, which expressed alkaline phophatase weakly but not Osteopontin and Osteocalcin, and a few immature osteoclasts ..
  4. ncbi The novel zinc finger-containing transcription factor osterix is required for osteoblast differentiation and bone formation
    Kazuhisa Nakashima
    Department of Molecular Genetics and Program in Genes and Development, M D Anderson Cancer Center, University of Texas, 1515 Holcombe Boulevard, Houston, TX 77030, USA
    Cell 108:17-29. 2002
    ..in the periosteum and in the condensed mesenchyme of membranous skeletal elements cannot differentiate into osteoblasts. These cells do, however, express Runx2/Cbfa1, another transcription factor required for bone formation...
  5. ncbi Osteoblastic cells regulate the haematopoietic stem cell niche
    L M Calvi
    Endocrine Unit, Department of Medicine, Center for Human Genetics and Molecular Pediatric Disease, University of Rochester School of Medicine, Rochester, New York 14642, USA
    Nature 425:841-6. 2003
    ..Furthermore, ligand-dependent activation of PPR with parathyroid hormone (PTH) increased the number of osteoblasts in stromal cultures, and augmented ex vivo primitive haematopoietic cell growth that was abrogated by gamma-..
  6. ncbi Osf2/Cbfa1: a transcriptional activator of osteoblast differentiation
    P Ducy
    Department of Molecular Genetics, M D Anderson Cancer Center, University of Texas, Houston 77030, USA
    Cell 89:747-54. 1997
    ..Osf2/Cbfa1 binds to and regulates the expression of multiple genes expressed in osteoblasts. Finally, forced expression of Osf2/Cbfa1 in nonosteoblastic cells induces the expression of the principal ..
  7. ncbi Wnt/beta-catenin signaling in mesenchymal progenitors controls osteoblast and chondrocyte differentiation during vertebrate skeletogenesis
    Timothy F Day
    Geneti Disease Research Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Dev Cell 8:739-50. 2005
    Chondrocytes and osteoblasts are two primary cell types in the skeletal system that are differentiated from common mesenchymal progenitors...
  8. ncbi Identification of the haematopoietic stem cell niche and control of the niche size
    Jiwang Zhang
    Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA
    Nature 425:836-41. 2003
    ..We conclude that SNO cells lining the bone surface function as a key component of the niche to support HSCs, and that BMP signalling through BMPRIA controls the number of HSCs by regulating niche size...
  9. ncbi Cbfa1, a candidate gene for cleidocranial dysplasia syndrome, is essential for osteoblast differentiation and bone development
    F Otto
    Imperial Cancer Research Fund, Lincoln s Inn Fields, London, United Kingdom
    Cell 89:765-71. 1997
    ..Analysis of their skeletons revealed an absence of osteoblasts and bone...
  10. ncbi Canonical Wnt signaling in differentiated osteoblasts controls osteoclast differentiation
    Donald A Glass
    Department of Molecular and Human Genetics, Bone Disease Program of Texas, Houston, 77030, USA
    Dev Cell 8:751-64. 2005
    ..signaling controls osteoblast differentiation and suggest that it may regulate bone formation in differentiated osteoblasts. Here, we study later events and find that stabilization of beta-catenin in differentiated osteoblasts results ..
  11. ncbi Tie2/angiopoietin-1 signaling regulates hematopoietic stem cell quiescence in the bone marrow niche
    Fumio Arai
    Department of Cell Differentiation, The Sakaguchi Laboratory of Developmental Biology, School of Medicine, Keio University, 35 Shinano machi, Shinjuku ku, Tokyo, 160 8582, Japan
    Cell 118:149-61. 2004
    ..kinase Tie2 are quiescent and antiapoptotic, and comprise a side-population (SP) of HSCs, which adhere to osteoblasts (OBs) in the BM niche...
  12. pmc Regulation of bone mass by Wnt signaling
    Venkatesh Krishnan
    Musculoskeletal Research, Lilly Research Laboratories, Indianapolis, Indiana 462385, USA
    J Clin Invest 116:1202-9. 2006
    ..In this Review we discuss the current understanding of this pathway in terms of bone biology and assess whether targeting this pathway might yield novel therapeutics to treat typical bone disorders...
  13. pmc Stem cell fate dictated solely by altered nanotube dimension
    Seunghan Oh
    Department of Materials Science and Engineering, University California at San Diego, La Jolla, CA 92093, USA
    Proc Natl Acad Sci U S A 106:2130-5. 2009
    ..either augmented human mesenchymal stem cell (hMSC) adhesion or a specific differentiation of hMSCs into osteoblasts by using only the geometric cues, absent of osteogenic inducing media...
  14. ncbi Building strong bones: molecular regulation of the osteoblast lineage
    Fanxin Long
    Departments of Medicine and Developmental Biology, Washington University School of Medicine, St Louis, MO 63110, USA
    Nat Rev Mol Cell Biol 13:27-38. 2012
    The past 15 years have witnessed tremendous progress in the molecular understanding of osteoblasts, the main bone-forming cells in the vertebrate skeleton...
  15. ncbi Investigation of multipotent postnatal stem cells from human periodontal ligament
    Byoung Moo Seo
    Craniofacial and Skeletal Diseases Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Department of Health and Human Services, Bethesda, MD, USA
    Lancet 364:149-55. 2004
    ..We investigated the notion that human PDL contains stem cells that could be used to regenerate periodontal tissue...
  16. pmc Matrix-embedded cells control osteoclast formation
    Jinhu Xiong
    Center for Osteoporosis and Metabolic Bone Diseases, University of Arkansas for Medical Sciences UAMS, Little Rock, Arkansas, USA
    Nat Med 17:1235-41. 2011
    Osteoclasts resorb the mineralized matrices formed by chondrocytes or osteoblasts. The cytokine receptor activator of nuclear factor-κB ligand (RANKL) is essential for osteoclast formation and thought to be supplied by osteoblasts or ..
  17. ncbi Mechanisms of bone metastasis
    G David Roodman
    Department of Medicine, Division of Hematology Oncology, University of Pittsburgh, School of Medicine Hematology, The University of Pittsburgh Cancer Institute, Pittsburgh, PA 15213, USA
    N Engl J Med 350:1655-64. 2004
  18. ncbi Thrombin induces epidermal growth factor receptor transactivation and CCL2 expression in human osteoblasts
    Chun Yin Huang
    China Medical University and China Medical University Hospital, Taichung, Taiwan
    Arthritis Rheum 64:3344-54. 2012
    ..Abnormalities in these processes are primary features of rheumatoid arthritis (RA). The aim of this study was to investigate the intracellular signaling pathways involved in thrombin-induced CCL2 expression in human osteoblasts.
  19. pmc A microRNA signature for a BMP2-induced osteoblast lineage commitment program
    Zhaoyong Li
    Department of Cell Biology and Cancer Center, University of Massachusetts Medical School, Worcester, MA 01655, USA
    Proc Natl Acad Sci U S A 105:13906-11. 2008
    ..Thus, our studies establish a mechanism for BMP morphogens to selectively induce a tissue-specific phenotype and suppress alternative lineages...
  20. ncbi Birth and death of bone cells: basic regulatory mechanisms and implications for the pathogenesis and treatment of osteoporosis
    S C Manolagas
    Division of Endocrinology and Metabolism, University of Arkansas for Medical Sciences, Little Rock 72205, USA
    Endocr Rev 21:115-37. 2000
    ..The adult skeleton regenerates by temporary cellular structures that comprise teams of juxtaposed osteoclasts and osteoblasts and replace periodically old bone with new...
  21. pmc Pathogenesis of osteoporosis: concepts, conflicts, and prospects
    Lawrence G Raisz
    University of Connecticut Health Center, Musculoskeletal Institute, Farmington, Connecticut 06032, USA
    J Clin Invest 115:3318-25. 2005
    ..However, new and more effective approaches are likely to emerge from a better understanding of the regulators of bone cell function...
  22. ncbi Distinct roles for Hedgehog and canonical Wnt signaling in specification, differentiation and maintenance of osteoblast progenitors
    Stephen J Rodda
    Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Avenue, Cambridge, MA 02138, USA
    Development 133:3231-44. 2006
    ..of Hh responsiveness in later Runx2+, Osx1+ osteoblast precursors does not disrupt the formation of mature osteoblasts. By contrast, the removal of canonical Wnt signaling by conditional removal of the beta-catenin gene in early ..
  23. ncbi Modulation of osteoclast differentiation and function by the new members of the tumor necrosis factor receptor and ligand families
    T Suda
    Department of Biochemistry, School of Dentistry, Showa University, Tokyo, Japan
    Endocr Rev 20:345-57. 1999
    b>Osteoblasts/stromal cells are essentially involved in osteoclast differentiation and function through cell-to-cell contact (Fig. 8)...
  24. ncbi Control of osteoblast function and regulation of bone mass
    Shun ichi Harada
    Department of Bone Biology and Osteoporosis Research, Merck Research Laboratories, West Point, Pennsylvania 19002, USA
    Nature 423:349-55. 2003
    ..Recent advances, spearheaded by genetic information, offer the opportunity to stop or reverse this downhill course...
  25. pmc Indian hedgehog signaling regulates proliferation and differentiation of chondrocytes and is essential for bone formation
    B St-Jacques
    Harvard University, Department of Molecular and Cellular Biology, Cambridge, Massachusetts 02138 USA
    Genes Dev 13:2072-86. 1999
    ..Together, the results suggest a model in which Ihh coordinates diverse aspects of skeletal morphogenesis through PTHrP-dependent and independent processes...
  26. ncbi Transforming growth factor-beta1 to the bone
    Katrien Janssens
    Department of Medical Genetics, University of Antwerp, Campus Drie Eiken, 2610 Antwerp, Belgium
    Endocr Rev 26:743-74. 2005
    ..This review provides an overall picture of the bone-specific actions of TGF-beta1 and reconciles experimental discrepancies that have been reported for this multifunctional cytokine...
  27. ncbi Adipocyte differentiation of bone marrow-derived mesenchymal stem cells: cross talk with the osteoblastogenic program
    S Muruganandan
    Department of Pharmacology, Dalhousie University, Halifax, Nova Scotia, B3H 1X5, Canada
    Cell Mol Life Sci 66:236-53. 2009
    ..stem cells (MSCs) are multipotent cells, which among other cell lineages, give rise to adipocytes and osteoblasts. Within the bone marrow, the differentiation of MSCs into adipocytes or osteoblasts is competitively balanced; ..
  28. ncbi Osteoblast adhesion on biomaterials
    K Anselme
    Institut de Recherche sur les Maladies du Squelette, Institut Calot, Berck sur Mer, France
    Biomaterials 21:667-81. 2000
    ....
  29. ncbi Regulation of osteogenic differentiation during skeletal development
    Zhong Liang Deng
    Key Laboratory of Diagnostic Medicine and the Affiliated Hospitals of Chongqing Medical University, Chongqing 400046, China
    Front Biosci 13:2001-21. 2008
    ..Bone is also continuously remodeled in vertebrates throughout life. Osteoblasts and osteoclasts are specialized cells responsible for bone formation and resorption, respectively...
  30. ncbi Essential role of beta-catenin in postnatal bone acquisition
    Sheri L Holmen
    Laboratory of Cell Signaling and Carcinogenesis, Van Andel Research Institute, Grand Rapids, Michigan 49503, USA
    J Biol Chem 280:21162-8. 2005
    ..in osteogenesis, we generated mice lacking the beta-catenin or adenomatous polyposis coli (Apc) genes in osteoblasts. Loss of beta-catenin produced severe osteopenia with striking increases in osteoclasts, whereas constitutive ..
  31. pmc Insulin signaling in osteoblasts integrates bone remodeling and energy metabolism
    Mathieu Ferron
    Department of Genetics and Development, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA
    Cell 142:296-308. 2010
    ..We show here that insulin signaling in osteoblasts is necessary for whole-body glucose homeostasis because it increases osteocalcin activity...
  32. ncbi Conditional inactivation of FGF receptor 2 reveals an essential role for FGF signaling in the regulation of osteoblast function and bone growth
    Kai Yu
    Department of Molecular Biology and Pharmacology, Washington University Medical School, Campus Box 8103, 660 S Euclid Avenue, St Louis, Missouri 63110, USA
    Development 130:3063-74. 2003
    ..Although differentiation of the osteoblast lineage was not disturbed, the proliferation of osteoprogenitors and the anabolic function of mature osteoblasts were severely affected.
  33. ncbi Aging is associated with decreased maximal life span and accelerated senescence of bone marrow stromal cells
    Karin Stenderup
    University Department of Endocrinology and Metabolism, University Hospital of Aarhus, DK 8000 Aarhus C, Denmark
    Bone 33:919-26. 2003
    ..In conclusion, aging is associated with decreased proliferative capacity of osteoprogenitor cells, suggesting that decreased osteoblastic cell number, and not function, leads to age-related decrease in bone formation...
  34. pmc Age-related intrinsic changes in human bone-marrow-derived mesenchymal stem cells and their differentiation to osteoblasts
    Shuanhu Zhou
    Department of Orthopedic Surgery, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Aging Cell 7:335-43. 2008
    ..MSCs, also known as mesenchymal stem cells) has potential to differentiate into multiple cell types, including osteoblasts. In this study, we tested the hypothesis that there are intrinsic effects of age in human MSCs (17-90 years)...
  35. ncbi Canonical WNT signaling promotes osteogenesis by directly stimulating Runx2 gene expression
    Tripti Gaur
    Department of Cell Biology and the Cancer Center, University of Massachusetts Medical School, Worcester, Massachusetts 01655 0106, USA
    J Biol Chem 280:33132-40. 2005
    ..We propose that WNT/TCF1 signaling, like bone morphogenetic protein/transforming growth factor-beta signaling, activates Runx2 gene expression in mesenchymal cells for the control of osteoblast differentiation and skeletal development...
  36. ncbi TAZ, a transcriptional modulator of mesenchymal stem cell differentiation
    Jeong Ho Hong
    Center for Cancer Research, Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, E18 580, Cambridge, MA 02139, USA
    Science 309:1074-8. 2005
    ..Runx2 and peroxisome proliferator-activated receptor gamma (PPARgamma), drive MSCs to differentiate into either osteoblasts or adipocytes, respectively...
  37. pmc Wnt/β-catenin signaling activates bone morphogenetic protein 2 expression in osteoblasts
    Rongrong Zhang
    Department of Biostatistics and Bioinformatics, Tulane University, New Orleans, LA 70112, USA
    Bone 52:145-56. 2013
    ..gene expression of the Wnt pathway, much less is known about whether Wnt signaling modulates BMP expression in osteoblasts. Given the presence of putative Tcf/Lef response elements that bind β-catenin/TCF transcription complex in the ..
  38. pmc Annexin II expressed by osteoblasts and endothelial cells regulates stem cell adhesion, homing, and engraftment following transplantation
    Younghun Jung
    Department of Periodontics and Oral Medicine, University of Michigan School of Dentistry, Ann Arbor, MI 48109 1078, USA
    Blood 110:82-90. 2007
    ..HSCs) after birth is largely restricted to the bone marrow cavity, where HSCs are associated closely with osteoblasts (OBs). How OBs localize HSCs to the endosteal niche remains unclear...
  39. ncbi Bone regenerates via dedifferentiation of osteoblasts in the zebrafish fin
    Franziska Knopf
    Biotechnology Center and Center for Regenerative Therapies, University of Technology Dresden, Tatzberg 47 49, 01307 Dresden, Germany
    Dev Cell 20:713-24. 2011
    ..We show that mature osteoblasts dedifferentiate and form part of the blastema...
  40. ncbi Distinct roles of bone morphogenetic proteins in osteogenic differentiation of mesenchymal stem cells
    Hue H Luu
    Molecular Oncology Laboratory, Department of Surgery, 5841 South Maryland Avenue, MC 3079, Room J 611, The University of Chicago Medical Center, Chicago, Illinois 60637, USA
    J Orthop Res 25:665-77. 2007
    ..It is conceivable that osteogenic BMPs (i.e., BMP2, 4, 6, 7, and 9) may be used to formulate synergistic pairs among themselves and/or with other less osteogenic BMPs for efficacious bone regeneration in clinical settings...
  41. ncbi Haematopoietic stem cell release is regulated by circadian oscillations
    Simon Mendez-Ferrer
    Mount Sinai School of Medicine, Department of Medicine and Department of Gene and Cell Medicine, New York, New York 10029, USA
    Nature 452:442-7. 2008
    ..These data indicate that a circadian, neurally driven release of HSC during the animal's resting period may promote the regeneration of the stem cell niche and possibly other tissues...
  42. ncbi Oxidative stress inhibits osteoblastic differentiation of bone cells by ERK and NF-kappaB
    Xiao Chun Bai
    Department of Cell Biology, The First Military Medical University, Guangzhou, PR China
    Biochem Biophys Res Commun 314:197-207. 2004
    ..the osteoblastic differentiation process of primary rabbit bone marrow stromal cells (BMSC) and calvarial osteoblasts, manifested by a reduction of differentiation markers including alkaline phosphatase (ALP), type I collagen, ..
  43. pmc Multiple functions of Osterix are required for bone growth and homeostasis in postnatal mice
    Xin Zhou
    Department of Genetics, University of Texas M D Anderson Cancer Center, Houston, TX 77030, USA
    Proc Natl Acad Sci U S A 107:12919-24. 2010
    ..Our study identifies Osx as an essential multifunctional player in postnatal bone growth and homeostasis...
  44. pmc Regeneration of amputated zebrafish fin rays from de novo osteoblasts
    Sumeet Pal Singh
    Department of Cell Biology and Howard Hughes Medical Institute, Duke University Medical Center, Durham, NC 27710, USA
    Dev Cell 22:879-86. 2012
    ..indicated that zebrafish fin ray bone regenerates through the dedifferentiation and proliferation of spared osteoblasts, with limited if any contribution from other cell types...
  45. ncbi Bone development
    B R Olsen
    Harvard Medical School, Department of Cell Biology, 240 Longwood Avenue, Boston, Massachusetts 02115, USA
    Annu Rev Cell Dev Biol 16:191-220. 2000
    ..Within these condensations, cells differentiate to chondrocytes or osteoblasts and form cartilages and bones under the control of various transcription factors...
  46. pmc Osteoblast precursors, but not mature osteoblasts, move into developing and fractured bones along with invading blood vessels
    Christa Maes
    Massachusetts General Hospital and Harvard Medical School, Boston, 02114, USA
    Dev Cell 19:329-44. 2010
    During endochondral bone development, the first osteoblasts differentiate in the perichondrium surrounding avascular cartilaginous rudiments; the source of trabecular osteoblasts inside the later bone is, however, unknown...
  47. ncbi Leptin regulation of bone resorption by the sympathetic nervous system and CART
    Florent Elefteriou
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA
    Nature 434:514-20. 2005
    ..which vertebrates regulate bone mass, comprises two phases, namely resorption by osteoclasts and formation by osteoblasts; osteoblasts are multifunctional cells also controlling osteoclast differentiation...
  48. ncbi Signals from the sympathetic nervous system regulate hematopoietic stem cell egress from bone marrow
    Yoshio Katayama
    Department of Medicine, Immunobiology Center and Black Family Stem Cell Institute, Mount Sinai School of Medicine, New York, NY 10029, USA
    Cell 124:407-21. 2006
    ..Further, administration of a beta(2) adrenergic agonist enhances mobilization in both control and NE-deficient mice. Thus, these results indicate that the sympathetic nervous system regulates the attraction of stem cells to their niche...
  49. ncbi Regulation of bone development and extracellular matrix protein genes by RUNX2
    Toshihisa Komori
    Department of Cell Biology, Nagasaki University Graduate School of Biomedical Sciences, Japan
    Cell Tissue Res 339:189-95. 2010
    ..transcription factor that controls skeletal development by regulating the differentiation of chondrocytes and osteoblasts and the expression of many extracellular matrix protein genes during chondrocyte and osteoblast differentiation...
  50. pmc The stem cell niches in bone
    Tong Yin
    Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA
    J Clin Invest 116:1195-201. 2006
    ..In adults, osteoblasts, responsible for osteogenesis, and hematopoietic cells, responsible for hematopoiesis, are closely associated ..
  51. ncbi Axial prevascularization of porous matrices using an arteriovenous loop promotes survival and differentiation of transplanted autologous osteoblasts
    Andreas Arkudas
    Department of Plastic and Hand Surgery, University of Erlangen Medical Center, Erlangen, Germany
    Tissue Eng 13:1549-60. 2007
    ..prevascularization of porous hard matrices using an arteriovenous (AV) loop promotes survival of transplanted osteoblasts. A processed bovine cancellous bone matrix was inserted into the AV loop...
  52. ncbi Inhibitor of DNA binding/differentiation helix-loop-helix proteins mediate bone morphogenetic protein-induced osteoblast differentiation of mesenchymal stem cells
    Ying Peng
    Molecular Oncology Laboratory, Department of Surgery, and Committe on Genetics, University of Chicago, IL 60637, USA
    J Biol Chem 279:32941-9. 2004
    ..progenitor cells and that Id expression must be down-regulated during the terminal differentiation of committed osteoblasts, suggesting that a balanced regulation of Id expression may be critical to BMP-induced osteoblast lineage-..
  53. pmc Update on Wnt signaling in bone cell biology and bone disease
    David G Monroe
    Department of Medicine Endocrine Research Unit, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA
    Gene 492:1-18. 2012
    ..Here we review recent advances and discrepancies in how Wnt/Lrp5 signaling regulates osteoblasts and osteocytes, introduce new players in Wnt signaling pathways that have important roles in bone development, ..
  54. pmc BMP2 regulates Osterix through Msx2 and Runx2 during osteoblast differentiation
    Takuma Matsubara
    Department of Molecular and Cellular Biochemistry, Osaka University Graduate School of Dentistry, Suita, Osaka 565 0871, Japan
    J Biol Chem 283:29119-25. 2008
    ..phosphatase activity and osteocalcin expression in C2C12 cells and stimulated calcification of murine primary osteoblasts. Considering that Runx2 overexpression induces Osterix, these results suggest that Osterix functions as ..
  55. ncbi Sequential roles of Hedgehog and Wnt signaling in osteoblast development
    Hongliang Hu
    Department of Medicine, Washington University Medical School, St Louis, MO 63110, USA
    Development 132:49-60. 2005
    ..Finally Wnt7b is identified as a potential endogenous ligand regulating osteogenesis. These data support a model that integrates Hh and Wnt signaling in the regulation of osteoblast development...
  56. pmc Insulin receptor signaling in osteoblasts regulates postnatal bone acquisition and body composition
    Keertik Fulzele
    Department of Orthopaedic Surgery, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Cell 142:309-19. 2010
    ..Here, we show that insulin receptor (IR) signaling in osteoblasts controls osteoblast development and osteocalcin expression by suppressing the Runx2 inhibitor Twist2...
  57. ncbi Stimulation of bone formation in vitro and in rodents by statins
    G Mundy
    OsteoScreen, 2040 Babcock Road, San Antonio, TX 78229, USA
    Science 286:1946-9. 1999
    ..Thus, in appropriate doses, statins may have therapeutic applications for the treatment of osteoporosis...
  58. ncbi Osteoblasts in prostate cancer metastasis to bone
    Christopher J Logothetis
    Department of Genitourinary Oncology, University of Texas, M D Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, Texas 77030, USA
    Nat Rev Cancer 5:21-8. 2005
    ..The interaction between host cells and metastatic cancer cells is an important component of organ-specific cancer progression. How can this knowledge lead to the development of more effective therapies?..
  59. pmc Bone progenitor dysfunction induces myelodysplasia and secondary leukaemia
    Marc H G P Raaijmakers
    Center for Regenerative Medicine, Massachusetts General Hospital and Harvard Medical School CPZN, USA
    Nature 464:852-7. 2010
    ..haematopoiesis, here we show that deletion of Dicer1 specifically in mouse osteoprogenitors, but not in mature osteoblasts, disrupts the integrity of haematopoiesis...
  60. ncbi PTH/PTHrP receptor in early development and Indian hedgehog-regulated bone growth
    B Lanske
    Endocrine Unit, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA
    Science 273:663-6. 1996
    ..These results suggest that the PTH/PTHrP receptor mediates the effects of Indian Hedgehog and PTHrP on chondrocyte differentiation...
  61. ncbi Thrombopoietin/MPL signaling regulates hematopoietic stem cell quiescence and interaction with the osteoblastic niche
    Hiroki Yoshihara
    Department of Cell Differentiation, The Sakaguchi Laboratory of Developmental Biology, Keio University, 35 Shinano machi, Tokyo, 160 8582, Japan
    Cell Stem Cell 1:685-97. 2007
    ..Altogether, these observations suggest that THPO/MPL signaling plays a critical role of LT-HSC regulation in the osteoblastic niche...
  62. ncbi Targeted ablation of osteocytes induces osteoporosis with defective mechanotransduction
    Sawako Tatsumi
    Department of Bone and Joint Disease, Research Institute, National Center for Geriatrics and Gerontology, Obu, Aichi 474 8522, Japan
    Cell Metab 5:464-75. 2007
    Bone remodeling is performed by osteoclasts and osteoblasts at the bone surface. Inside of bone is a network of numerous osteocytes, whose specific function has remained an enigma...
  63. pmc Tissue-nonspecific alkaline phosphatase and plasma cell membrane glycoprotein-1 are central antagonistic regulators of bone mineralization
    Lovisa Hessle
    The Burnham Institute, 10901 North Torrey Pines Road, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 99:9445-9. 2002
    b>Osteoblasts mineralize bone matrix by promoting hydroxyapatite crystal formation and growth in the interior of membrane-limited matrix vesicles (MVs) and by propagating the crystals onto the collagenous extracellular matrix...
  64. ncbi Reactive oxygen species stimulates receptor activator of NF-kappaB ligand expression in osteoblast
    Xiao Chun Bai
    Department of Cell Biology, Southern Medical University, Guangzhou 510515, China
    J Biol Chem 280:17497-506. 2005
    ..expression in human osteoblast-like MG63 cell line and primary mouse bone marrow stromal cells and calvarial osteoblasts. Further analysis revealed that ROS promoted phosphorylation of cAMP response element-binding protein (CREB)/..
  65. ncbi Aminobisphosphonates cause osteoblast apoptosis and inhibit bone nodule formation in vitro
    Aymen I Idris
    Rheumatic Diseases Unit, Molecular Medicine Centre, University of Edinburgh, General Western Hospital, Edinburgh EH4 2XU, UK
    Calcif Tissue Int 82:191-201. 2008
    ..and alendronate inhibited osteoblast growth, caused osteoblast apoptosis, and inhibited protein prenylation in osteoblasts in a dose-dependent manner over the concentration range 20-100 microM...
  66. pmc Cellular and molecular mechanisms of bone remodeling
    Liza J Raggatt
    Faculty of Health Sciences, The University of Queensland Centre for Clinical Research, Royal Brisbane Hospital, Herston 4029, Australia
    J Biol Chem 285:25103-8. 2010
    ..In addition to the traditional bone cells (osteoclasts, osteoblasts, and osteocytes) that are necessary for bone remodeling, several immune cells have also been implicated in bone ..
  67. ncbi Osteoinduction and survival of osteoblasts and bone-marrow stromal cells in 3D biphasic calcium phosphate scaffolds under static and dynamic culture conditions
    Subha N Rath
    Department of Plastic and Hand Surgery, University Hospital of Erlangen, Friedrich Alexander University of Erlangen Nurnberg, Erlangen, Germany
    J Cell Mol Med 16:2350-61. 2012
    ..To achieve this either primary rat osteoblasts or bone marrow stromal cells (BMSC) were implanted on uniform-sized biphasic calcium phosphate (BCP) scaffolds ..
  68. ncbi Replicative senescence of human bone marrow and umbilical cord derived mesenchymal stem cells and their differentiation to adipocytes and osteoblasts
    Huanchen Cheng
    Institute of Harbin Hematology and Oncology, Harbin First Hospital, Number 149 of Diduan Street, 150010 Harbin, People s Republic of China
    Mol Biol Rep 38:5161-8. 2011
    ..C/EBPα and PPARγ could regulate the balance of adipogenic differentiation in BM-MSC but only PPARγ not C/EBPα was involved in the adipogenic differentiation in UC-MSC...
  69. ncbi The essential functions of adipo-osteogenic progenitors as the hematopoietic stem and progenitor cell niche
    Yoshiki Omatsu
    Department of Immunobiology and Hematology, Kyoto University, 53 Kawahara cho, Shogoin, Sakyo ku, Kyoto, Japan
    Immunity 33:387-99. 2010
    ..ligand (CXCL)12-abundant reticular (CAR) cells in vivo did not affect the candidate niches, bone-lining osteoblasts, or endothelial cells but severely impaired the adipogenic and osteogenic differentiation potential of marrow ..
  70. pmc miR-218 directs a Wnt signaling circuit to promote differentiation of osteoblasts and osteomimicry of metastatic cancer cells
    Mohammad Q Hassan
    Department of Cell Biology and Cancer Center, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    J Biol Chem 287:42084-92. 2012
    ..Thus, miR-218/Wnt signaling circuit amplifies both the osteoblast phenotype and osteomimicry-related tumor activity...
  71. pmc Transcriptional regulation of Vascular Endothelial Growth Factor (VEGF) by osteoblast-specific transcription factor Osterix (Osx) in osteoblasts
    Wanjin Tang
    Bone Research Laboratory, Texas Scottish Rite Hospital for Children, Dallas, Texas 75219, USA
    J Biol Chem 287:1671-8. 2012
    ..Transcriptional regulation of VEGF expression is not well known in osteoblasts. In this study, quantitative real-time RT-PCR results revealed that VEGF expression was down-regulated in Osx-..
  72. pmc Baicalin, a flavone, induces the differentiation of cultured osteoblasts: an action via the Wnt/beta-catenin signaling pathway
    Ava J Y Guo
    Division of Life Science and Center for Chinese Medicine, Hong Kong University of Science and Technology, Clear Water Bay Road, Hong Kong
    J Biol Chem 286:27882-93. 2011
    ..were screened for their osteogenic properties by measuring alkaline phosphatase activity in cultured rat osteoblasts. The flavone baicalin derived mainly from the roots of Scutellaria baicalensis showed the strongest induction ..
  73. pmc Adhesion of osteoblasts to a nanorough titanium implant surface
    Ekaterina Gongadze
    Institute of General Electrical Engineering, University of Rostock, Rostock, Germany
    Int J Nanomedicine 6:1801-16. 2011
    ..We suggest that osteoblasts are most strongly bound along the sharp convex edges or spikes of nanorough titanium surfaces where the ..
  74. ncbi The endosteal 'osteoblastic' niche and its role in hematopoietic stem cell homing and mobilization
    J P Levesque
    Biotherapies Program, Haematopoietic Stem Cell Laboratory, Mater Medical Research Institute, South Brisbane, Queensland, Australia
    Leukemia 24:1979-92. 2010
    ..as a preferential site of residence for the most potent HSC - able to reconstitute in serial transplants - with osteoblasts and their progenitors as critical cellular elements of these endosteal niches...
  75. ncbi Regulatory mechanisms of osteoblast and osteoclast differentiation
    T Katagiri
    Department of Biochemistry, School of Dentistry, Showa University, Japan
    Oral Dis 8:147-59. 2002
    ..b>Osteoblasts and osteoclasts are specialized cells responsible for bone formation and resorption, respectively...
  76. pmc Suppression of CXCL12 production by bone marrow osteoblasts is a common and critical pathway for cytokine-induced mobilization
    Matthew J Christopher
    Division of Oncology, Department of Medicine, Washington University School of Medicine, St Louis, MO 63110, USA
    Blood 114:1331-9. 2009
    ..Loss of CXCL12 expression by osteoblast appears to be a common and key step in cytokine-induced mobilization...
  77. ncbi Sclerostin is a delayed secreted product of osteocytes that inhibits bone formation
    Kenneth E S Poole
    MRC Bone Research Group, Department of Medicine, University of Cambridge, Addenbrooke s Hospital, Cambridge, UK
    FASEB J 19:1842-4. 2005
    ..secrete sclerostin after they become embedded in a mineralized matrix to limit further bone formation by osteoblasts. Sclerostin did not appear to influence the formation of osteocytes...
  78. ncbi Regulation of the osteoblast-specific transcription factor, Runx2: responsiveness to multiple signal transduction pathways
    Renny T Franceschi
    Department of Periodontics, School of Dentistry, Ann Arbor, Michigan 48109 1078, USA
    J Cell Biochem 88:446-54. 2003
    ..These findings suggest that Runx2 plays a central role in coordinating multiple signals involved in osteoblast differentiation...
  79. pmc Concerted regulation of inorganic pyrophosphate and osteopontin by akp2, enpp1, and ank: an integrated model of the pathogenesis of mineralization disorders
    Dympna Harmey
    The Burnham Institute, La Jolla, CA 92037, USA
    Am J Pathol 164:1199-209. 2004
    ..Wild-type osteoblasts treated with PP(i) showed an increase in OPN, and a decrease in Enpp1 and Ank expression...
  80. pmc Unique coexpression in osteoblasts of broadly expressed genes accounts for the spatial restriction of ECM mineralization to bone
    Monzur Murshed
    Department of Molecular and Human Genetics, Bone Disease Program of Texas, Baylor College of Medicine, Houston, TX 77030, USA
    Genes Dev 19:1093-104. 2005
    ..ECM mineralization occurs only in bone because of the exclusive coexpression in osteoblasts of Type I collagen and Tnap, an enzyme that cleaves pyrophosphate...
  81. pmc A novel microRNA targeting HDAC5 regulates osteoblast differentiation in mice and contributes to primary osteoporosis in humans
    Hui Li
    Institute of Endocrinology and Metabolism, Second Xiangya Hospital of Central South University, Changsha, Hunan, People s Republic of China
    J Clin Invest 119:3666-77. 2009
    ..Here, we identify a new miRNA (miR-2861) in primary mouse osteoblasts that promotes osteoblast differentiation by repressing histone deacetylase 5 (HDAC5) expression at the post-..
  82. pmc Mechanical strain regulates osteoblast proliferation through integrin-mediated ERK activation
    Yu Xian Yan
    Institute of Medical Equipment, Academy of Military Medical Science, Tianjin, China
    PLoS ONE 7:e35709. 2012
    ..As mechanical receptor cells, osteoblasts perceive and respond to stress force, such as those associated with compression, strain and shear stress...
  83. pmc Primary cilia mediate mechanosensing in bone cells by a calcium-independent mechanism
    Amanda M D Malone
    Palo Alto Veterans Administration Medical Center, Palo Alto, CA 94304, USA
    Proc Natl Acad Sci U S A 104:13325-30. 2007
    ..Our identification of a mechanism for mechanotransduction in bone could lead to therapeutic approaches for combating bone loss due to osteoporosis and disuse...
  84. ncbi Deletion of the P2X7 nucleotide receptor reveals its regulatory roles in bone formation and resorption
    Hua Zhu Ke
    Pfizer Global Research and Development, Groton Laboratories, Connecticut 06340, USA
    Mol Endocrinol 17:1356-67. 2003
    ..Functional P2X7 receptors were also found in osteoblasts from WT but not KO mice, suggesting a direct role in bone formation...
  85. ncbi IL-6, RANKL, TNF-alpha/IL-1: interrelations in bone resorption pathophysiology
    Steeve Kwan Tat
    Laboratoire de Physiopathologie de la Résorption Osseuse et Thérapie des Tumeurs Osseuses Primitives, Faculte de Medecine, 1 rue Gaston Veil, BP 53508, 44035 Nantes 1, France
    Cytokine Growth Factor Rev 15:49-60. 2004
    All osteogenic cells (osteoclasts, osteoblasts) contribute individually to bone remodeling. Their cellular interactions control their cellular activities and the bone remodeling intensity...
  86. pmc Osteoblasts derived from induced pluripotent stem cells form calcified structures in scaffolds both in vitro and in vivo
    Ganna Bilousova
    Charles C Gates Regenerative Medicine and Stem Cell Biology Program, University of Colorado Denver, Aurora, Colorado 80045, USA
    Stem Cells 29:206-16. 2011
    ..After 12 weeks of expansion, iPS-derived osteoblasts were seeded in a gelfoam matrix followed by subcutaneous implantation in syngenic imprinting control region (..
  87. ncbi Chitosan enhances mineralization during osteoblast differentiation of human bone marrow-derived mesenchymal stem cells, by upregulating the associated genes
    S Mathews
    Manipal Institute of Regenerative Medicine, Manipal University, Domlur, Bangalore, India Kasiak Research Pvt Ltd, Makers Chamber VI, Nariman Point, Mumbai, India
    Cell Prolif 44:537-49. 2011
    ..Here, we have evaluated effects of chitosan-coated tissue culture plates on adhesion and osteoblast differentiation processes of human mesenchymal stem cells (hMSCs), isolated from adult bone marrow...
  88. ncbi Increased sclerostin serum levels associated with bone formation and resorption markers in patients with immobilization-induced bone loss
    Agostino Gaudio
    Department of Internal Medicine, University of Catania, 95124 Catania, Italy
    J Clin Endocrinol Metab 95:2248-53. 2010
    Sclerostin, a Wnt signaling antagonist on the osteoblasts produced by osteocytes, is regulated by mechanical strain and is implicated in the pathogenesis of disuse bone loss. There are no data on sclerostin in humans.
  89. ncbi Detection of functional haematopoietic stem cell niche using real-time imaging
    Yucai Xie
    Stowers Institute for Medical Research, 1000 E 50th Street, Kansas City, Missouri 64110, USA
    Nature 457:97-101. 2009
    ..Thus, in contrast to central marrow, the endosteum formed a special zone, which normally maintains HSCs but promotes their expansion in response to bone marrow damage...
  90. ncbi Networks and hubs for the transcriptional control of osteoblastogenesis
    Jane B Lian
    Department of Cell Biology and Cancer Center, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA
    Rev Endocr Metab Disord 7:1-16. 2006
    ..The specificity of Runx2 regulatory activities provides a basis for novel therapeutic strategies to correct bone disorders...
  91. ncbi Sclerostin mediates bone response to mechanical unloading through antagonizing Wnt/beta-catenin signaling
    Chuwen Lin
    Bio X Center, Key Laboratory of Developmental Genetics and Neuropsychiatric Diseases, Ministry of Education, Shanghai Jiao Tong University, Shanghai, China
    J Bone Miner Res 24:1651-61. 2009
    ..inhibited Wnt/beta-catenin in vivo, and sclerostin suppressed the activity of osteoblast and viability of osteoblasts and osteocytes. Interestingly, Sost(-/-) mice were resistant to mechanical unloading-induced bone loss...
  92. ncbi Regulation of osteoblast differentiation by transcription factors
    Toshihisa Komori
    Department of Developmental and Reconstructive Medicine, Division of Cell Biology, Nagasaki University Graduate School of Biomedical Sciences, 1 7 1 Sakamoto, Nagasaki 852 8588, Japan
    J Cell Biochem 99:1233-9. 2006
    ..After differentiating to preosteoblasts, beta-catenin, osterix, and Runx2 direct them to immature osteoblasts, which produce bone matrix proteins, blocking their potential to differentiate into the chondrocytic lineage...
  93. pmc The p38 MAPK pathway is essential for skeletogenesis and bone homeostasis in mice
    Matthew B Greenblatt
    Department of Immunology and Infectious Diseases, Harvard School of Public Health, and Department of Medicine, Harvard Medical School, Boston, Massachusetts, USA
    J Clin Invest 120:2457-73. 2010
    ..TGF-beta-activated kinase 1 (TAK1; also known as MAP3K7) as the critical activator upstream of p38 in osteoblasts. Osteoblast-specific deletion of Tak1 resulted in clavicular hypoplasia and delayed fontanelle fusion, a ..
  94. pmc Osteopontin is a hematopoietic stem cell niche component that negatively regulates stem cell pool size
    Sebastian Stier
    Center for Regenerative Medicine and Technology, Massachusetts General Hospital Cancer Center, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA
    J Exp Med 201:1781-91. 2005
    ..b>Osteoblasts that participate in the niche produce varying amounts of OPN in response to stimulation...
  95. ncbi Glucocorticoids suppress bone formation by attenuating osteoblast differentiation via the monomeric glucocorticoid receptor
    Alexander Rauch
    Tissue specific hormone action, Leibniz Institute for Age Research, Fritz Lipmann Institute, 07745 Jena, Germany
    Cell Metab 11:517-31. 2010
    ..that GCs are unable to repress bone formation in the absence of glucocorticoid receptor (GR) expression in osteoblasts as they become refractory to hormone-induced apoptosis, inhibition of proliferation, and differentiation...
  96. pmc Cellular mechanisms of bone remodeling
    Erik Fink Eriksen
    Department of Clinical Endocrinology, Oslo University Hospital, Aker, Trondheimsveien 235, 0514 Oslo, Norway
    Rev Endocr Metab Disord 11:219-27. 2010
    ..The dominant pathway regulating osteoclast recruitment is the RANKL/OPG system, while many different factors (RUNX, Osterix) are involved in osteoblast differentiation. Both pathways are modulated by calcitropic hormones...
  97. pmc P2X7 receptors on osteoblasts couple to production of lysophosphatidic acid: a signaling axis promoting osteogenesis
    Nattapon Panupinthu
    Canadian Institutes of Health Research Group in Skeletal Development and Remodeling, London, ON, Canada
    J Cell Biol 181:859-71. 2008
    ..We find that calvarial sutures are wider in P2rx7-/- mice. Functional P2X7 receptors are expressed on osteoblasts in situ and in vitro...
  98. pmc Notch signaling maintains bone marrow mesenchymal progenitors by suppressing osteoblast differentiation
    Matthew J Hilton
    Department of Medicine, Washington University School of Medicine, 660 South Euclid Avenue, St Louis, Missouri 63110, USA
    Nat Med 14:306-14. 2008
    ..Thus, mesenchymal progenitors may be expanded in vitro by activating the Notch pathway, whereas bone formation in vivo may be enhanced by transiently suppressing this pathway...
  99. ncbi Extracellular nucleotides block bone mineralization in vitro: evidence for dual inhibitory mechanisms involving both P2Y2 receptors and pyrophosphate
    Isabel R Orriss
    Department of Anatomy and Developmental Biology, University College London, London WC1E 6BT, United Kingdom
    Endocrinology 148:4208-16. 2007
    ..inhibitor of mineralization) on the deposition and mineralization of collagenous matrix by primary osteoblasts derived from rat calvariae...
  100. pmc Critical role of the extracellular signal-regulated kinase-MAPK pathway in osteoblast differentiation and skeletal development
    Chunxi Ge
    Department of Periodontics and Oral Medicine, University of Michigan School of Dentistry, Ann Arbor, MI 48109, USA
    J Cell Biol 176:709-18. 2007
    ..MAPK stimulation achieved by selective expression of constitutively active MAPK/ERK1 (MEK-SP) in osteoblasts accelerated in vitro differentiation of calvarial cells, as well as in vivo bone development, whereas dominant-..
  101. pmc Cyclooxygenase-2 regulates mesenchymal cell differentiation into the osteoblast lineage and is critically involved in bone repair
    Xinping Zhang
    The Center for Musculoskeletal Research, University of Rochester Medical Center, New York 14642, USA
    J Clin Invest 109:1405-15. 2002
    ..These results provide a model whereby COX-2 regulates the induction of cbfa1 and osterix to mediate normal skeletal repair...

Research Grants74

  1. Bone Deficits and Excess Adiposity after Pediatric Bone Marrow Transplantation
    Sogol Mostoufi-Moab; Fiscal Year: 2013
    ..cavity, where hormones and transcription factors regulate mesenchyme stem cell (MSC) differentiation into osteoblasts or osteocytes...
  2. Biology of IGFs in Bone
    Thomas L Clemens; Fiscal Year: 2013
    ..During the last funding period, we used mice lacking either IGF-1 or insulin receptor in osteoblasts to begin to distinguish actions of IGF-1 and insulin in the skeleton...
  3. Osteocalcin and Metabolic Risk Factors. The Framingham Study
    Yi Hsiang Hsu; Fiscal Year: 2011
    ..discovery in rodents, for the first time, osteocalcin (OC), a major bone formation protein uniquely secreted by osteoblasts, may play a crucial role in endocrine regulation of energy metabolism by enhancing b-cell proliferation, ..
  4. Sirtuin/FOXO Signaling in the Regulation of Bone Mass
    Stavroula Kousteni; Fiscal Year: 2012
    ..We have found that FOXO1 and SIRT1 exert direct anti-apoptotic or proliferative effects on osteoblasts and osteoblast precursors. They are also expressed in osteoclasts...
  5. Novel, Rapidly Translatable Technologies for Healing Long Bone Segmental Defects
    Christopher H Evans; Fiscal Year: 2010
    ..cells within the healed bone will be further probed using antibodies against human Runx2, to identify human osteoblasts, and against human tartrate-resistant acid phosphatase, to identify human osteoclasts...
  6. BBP (Bone Morphogenetic Protein Binding Peptide) and Bone Healing
    SAMUEL SCOTT MURRAY; Fiscal Year: 2013
    ..cytokines stimulate pluripotent mesenchymal cells to proliferate and differentiate into the chondrocytes and osteoblasts that elaborate cartilage and bone...
  7. Identification of Proteins Important for Male Osteoporosis
    Hong Wen Deng; Fiscal Year: 2013
    ..Bone marrow mesenchymal stem cells (BMMSCs) and peripheral blood monocytes (PBMs) are precursors for osteoblasts (bone formation cells) and osteoclasts (bone resorption cells), respectively...
  8. Runx Proteins and Sex Steroid Signaling in Bone
    Baruch Frenkel; Fiscal Year: 2009
    ..In humans, Runx2 haplotypes contribute to variations in bone mass. Runx1, which is expressed in osteoblasts and shares similar DNA-binding properties with Runx2, has been implicated in bone metabolism as well...
  9. Mechanisms of Stem Cell Self-Renewal and Aging
    PAUL CHRISTIAN SCHILLER; Fiscal Year: 2013
    ..Bone-forming osteoblasts originate from mesenchymal stromal cells (MSCs) in the bone marrow (BM)...
  10. Alcohol-Induced Bone Resorption: The Role of Oxidative Stress
    Martin J J Ronis; Fiscal Year: 2013
    ..RANKL is a member of the TNF family expressed on the surface of osteoblasts which signals through the receptor RANK on the surface of osteoclast precursors to stimulate osteoclastogenesis...
  11. Mechanisms of Bone Loss from Administration of the Second-Generation Antipsychoti
    KATHERINE JEAN MOTYL; Fiscal Year: 2013
    ..metabolism and homeostasis in a manner that results in impaired bone health as well as acts directly on osteoblasts and osteoclasts to disrupt normal skeletal modeling and remodeling...
  12. Undercarboxylated osteocalcin, body fat, and diabetes in older adults
    Ann V Schwartz; Fiscal Year: 2010
    New findings in mice suggest that bone affects the development of diabetes. A product of osteoblasts, undercarboxylated osteocalcin (ucOC), functions as a hormone regulating insulin sensitivity and production, an unexpected connection ..
  13. Salivary-based Bone-loss Marker Detection Platform for Point-of-Care Screening
    MANAL BESHAY; Fiscal Year: 2012
    ..Bone is a dynamic tissue that naturally and continually undergoes turnover with new bone formation by osteoblasts and old bone resorption by osteoclasts at a balanced rate;any imbalance between the two rates results in ..
  14. Effect of Adrenal and Gonadal Hormones on Bone Marrow and Appendicular BMD
    Catherine M Gordon; Fiscal Year: 2013
    ..in girls with AN influence mesenchymal stem cells to differentiate preferentially into adipocytes over osteoblasts. Enhancing understanding of the mechanisms underlying skeletal losses in this young patient population has the ..
  15. Misty: a model for central regulation of bone remodeling
    CLIFFORD JAMES ROSEN; Fiscal Year: 2013
    ..In bone, catecholamines bind to [unreadable]2 adrenergic receptors (AR) on osteoblasts to suppress bone formation and increase resorption while in BAT, catecholamines act on [unreadable]3 adrenergic ..
  16. Heat Shock Effects on Human Mesenchymal Stem Cell Differentiation in Hydrogel
    Sihong Wang; Fiscal Year: 2010
    ..inducers;and (2) to explore dynamic heat-shock effects on hMSC proliferation and differentiation into osteoblasts and chondrocytes in the optimized 3D culture environment, and to investigate the role of heat shock proteins (..
  17. Hematopoietic Stem Cell Transplantation in Osteogenesis Imperfecta
    Meenal Mehrotra; Fiscal Year: 2012
    ..Preclinical studies have demonstrated that bone marrow contains cells that can engraft and become competent osteoblasts after transplantation...
  18. Conditional Knockout of Calcium Receptors in Bone Cells
    Dolores M Shoback; Fiscal Year: 2013
    ..Cells in bone and marrow respond to fluxes in the [Ca2+]e which initiate signaling cascades. In osteoblasts, changes in the [Ca2+]e affect expression of genes critical for matrix production and mineralization, ..
  19. Use of umbilical cord as a unique stem cell source for tissue regeneration
    Xiao Dong Chen; Fiscal Year: 2013
    ..germ layers in vitro under conditions known to induce commitment to a specific cell lineage, including osteoblasts, adipocytes, chondroblasts and cardiomyocytes (mesoderm), nerve (ectoderm) and hepatocytes (endoderm)...
  20. Signal Transduction Mechanism of Osteoclast Differentiation
    Nandini Ghosh-Choudhury; Fiscal Year: 2013
    ..The bone remodeling occurs by concerted action of bone forming osteoblasts and bone resorbing osteoclasts...
  21. Sex Steroids and Runx Signaling in Bone
    Baruch Frenkel; Fiscal Year: 2013
    ..In humans, Runx2 haplotypes contribute to variations in bone mass. Runx1, which is expressed in osteoblasts and shares similar DNA-binding properties with Runx2, has been implicated in bone metabolism as well...
  22. Repression of PPARgamma-2 by Glucocorticoid-Induced Leucine Zipper
    Xing Ming Shi; Fiscal Year: 2012
    ..differentiation of bone marrow progenitor cells, the common precursors of fat- and bone-forming adipocytes and osteoblasts. Based on these studies and our recent preliminary data showing that GILZ interacts with the C/EBPs and ..
  23. Regulation of glucose metabolism by bone resorption and gamma-carboxylation
    Mathieu Ferron; Fiscal Year: 2012
    ..We have identified osteocalcin a hormone produced by the osteoblasts, the bone forming cells, as a previously uncharacterized regulator of insulin secretion and insulin sensitivity...
  24. Estrogens, androgens, aging, and bone loss in males
    Stavros C Manolagas; Fiscal Year: 2013
    ..of stimulating the nuclear- initiated actions of ER1 reproduces the effects of estradiol on osteoclasts and osteoblasts;and is as potent as estradiol in preventing oxidative stress and ovariectomy-induced bone loss, without ..
  25. Endothelin-1 and androgen in prostate cancer bone metastasis and bone health
    Gregory A Clines; Fiscal Year: 2013
    ..Antagonists that block the activation of the endothelin A receptor (ETAR), located on osteoblasts in bone, reduce osteoblastic bone lesions in animal models of bone metastasis...
  26. Nuclear Events in PTH Action on Bone
    Nicola C Partridge; Fiscal Year: 2013
    ..long-term goals of this work are to delineate the transcriptional regulatory mechanisms conveying PTH action in osteoblasts and bone...
  27. Analysis of the leukemic stem cell niche in chronic myeloid leukemia
    DANIELA SANDRA KRAUSE; Fiscal Year: 2013
    ..Furthermore, interaction of CML cells with their niche alters niche components like osteoblasts. The goals of my proposal therefore are: * To determine the microanatomy of the BM (BM) microenvironment in CML...
  28. cGMP-dependent Protein Kinase Function in Osteoblast Mechanotransduction
    Renate B Pilz; Fiscal Year: 2013
    ..NO donors and cGMP analogs mimicked the effect of FSS on Src/Erk in osteoblasts/cytes, while siRNA knock-down of membrane-bound PKG II abolished it...
  29. Novel Drug VS-105 for Treatment of Osteoporosis
    Jinshyun Ruth Wu-Wong; Fiscal Year: 2013
    ..absorption, parathyroid hormone, receptor activator of nuclear factor-kB ligand, and also via direct effects on osteoblasts and osteoclasts...
  30. Role of Gsalpha in Regulating Osteoblast Differentiation
    JOY Y WU; Fiscal Year: 2012
    ..The molecular mechanisms guiding the differentiation of bone forming cells, or osteoblasts, are not clearly understood...
  31. Dynamic Mechanisms of Vitamin D-Induced Gene Expression
    J Wesley Pike; Fiscal Year: 2012
    Bone is a dynamic tissue which is remodeled throughout life as a result of the biological activities of osteoblasts and osteoclasts...
  32. Roles of Gs Alpha and interaction with Wnt signaling in regulation of adipocyte d
    Partha S Sinha; Fiscal Year: 2011
    DESCRIPTION (provided by applicant): Adipocytes and osteoblasts may share a common precursor residing in the bone marrow...
  33. FUNCTION AND REGULATION OF OSTEONECTIN IN BONE
    Anne M Delany; Fiscal Year: 2013
    ..Mesenchymal stem cells (MSCs) give rise to both osteoblasts and adipocytes, and MSC lineage allocation is altered in aging...
  34. Effect of Polybrominated Diphenal Ether Flame Retardant Exposure on Osteogenesis
    Deena J Small; Fiscal Year: 2010
    ..to their activity as thyroid hormone receptor 11 (TR11) antagonists in immature and mature bone forming cells (osteoblasts)...
  35. ATF4 and Osteoclastogenesis
    Guozhi Xiao; Fiscal Year: 2013
    ..PUBLIC HEALTH RELEVANCE: Skeletal integrity requires a delicate balance between bone-forming osteoblasts and bone-resorbing osteoclasts (OCLs)...
  36. Role of SATB in placental development
    Mohammad A K Rumi; Fiscal Year: 2013
    ..They participate in transcriptional regulation of key genes controlling the differentiation of T lymphocytes, osteoblasts, cortical neurons, and embryonic stem cells...
  37. The role of caspase-2 in osteoblast-osteoclast interactions
    DANIELLE ARDEN VICTOR; Fiscal Year: 2013
    ..These factors will be measured from bone marrow cultures, containing osteoblasts and osteoclasts, generated from bone in young and old caspase-2 knockout and wild- type mice...
  38. Heparan Sulfate Proteoglycans in Human Hematopoiesis
    Pankaj Gupta; Fiscal Year: 2013
    ..In vivo, HSC reside in highly specialized "stem cell niches," likely formed by osteoblasts, wherein specific combinations of cytokines and matrix/cell surface macromolecules localize to regulate stem ..
  39. Regulation of IGF-1 and Insulin Signaling by O-GlcNAc Glycosylation
    LAUREN ELIZABETH BALL; Fiscal Year: 2013
    ..IGF-1 stimulation of osteoblasts, is anti-apoptotic and contributes to cell proliferation, differentiation, migration, matrix deposition and ..
  40. Mechanisms of FGFR2 Signaling in Craniofacial Development
    Veraragavan P Eswarakumar; Fiscal Year: 2013
    ..including FGFR2, which is expressed by proliferating osteoprogenitors and down-regulated in differentiating osteoblasts. Our preliminary data show that mutations in FGFR2 preferentially affect cranial and facial bones of neural ..
  41. Bc12 regulates osteoclast survival
    Junro Yamashita; Fiscal Year: 2010
    ..Osteoblast activity is not affected in vivo or ex vivo. Bcl2-/- osteoblasts are normal in their cellular activity, such as proliferation, differentiation, mineral formation, and ..
  42. Role of FoxOs in Skeletal Homeostasis
    MARIA JOSE ALMEIDA; Fiscal Year: 2013
    ..Finally, mice overexpressing FoxO3a in osteoblast precursors and their progeny (osteoblasts, osteocytes);and mice in which the three main FoxO isoforms FoxO1, 3a, and 4 are conditionally deleted will be ..
  43. Regulation of Osteoblast Function by Megakaryocytes: Key Signaling Proteins
    Melissa A Kacena; Fiscal Year: 2013
    ..However, our histological evaluation of GATA-1 and NF-E2 deficient mice reveals higher numbers of osteoblasts (OBs) on trabecular surfaces...
  44. The Roles of FAM20C (DMP4) in Odontogenesis and Osteogenesis
    Chunlin Qin; Fiscal Year: 2013
    ..of odontoblasts and ameloblasts;and 4) reduced expression of the differentiation markers for odontoblasts and osteoblasts [including dentin matrix protein 1 (DMP1), a differentiation/mineralization marker]...
  45. Nrf1 Molecular Pathway, Vitamin C Deficiency, and Spontaneous Fractures
    Subburaman Mohan; Fiscal Year: 2013
    ..for increased expression of osterix (Osx), a master osteogenic transcription factor, during differentiation of osteoblasts (OBs)...
  46. Osteoclasts from pluripotent stem cells as vectors to modify bone marrow environm
    Hector L Aguila; Fiscal Year: 2010
    ..We have shown that conditional ablation of osteoblasts modify hematopoietic niches allowing engraftment of exogenously transferred hematopoietic progenitors without ..
  47. Biomaterials (Mf/Zn/F-BCPs) for osteoporosis therapy
    Yu Zhang; Fiscal Year: 2012
    ..results when the rate of bone resorption (by osteoclasts) is much greater than the rate of bone formation (by osteoblasts) causing bone loss, deterioration of bone quality, leading to decreased bone strength and bone fragility and ..
  48. Inhibited Intramembranous Bone Healing in Diabetes
    Philip C Trackman; Fiscal Year: 2013
    ..that resembles osteopenic diabetic bone, and that lysyl oxidase is up-regulated by the Wnt canonical pathway in osteoblasts. Two specific aims are proposed...
  49. Connexin 43 in Bone Remodeling
    Roberto Civitelli; Fiscal Year: 2013
    ..Like remodeling, modeling requires coordination of osteoblasts (Obl), osteocytes (Ocy) and osteoclasts (Ocl)...
  50. Regulation of PTH-induced Osteoanabolism by Inflammatory Lipids
    Yin Tintut; Fiscal Year: 2013
    ..adipogenic lineages;anabolic responses of mature osteoblasts/osteocytes;and/or transient expression of osteoclastogenic factors by osteoblasts...
  51. PTHrP Modulation of TRPV1 in Pain Associated with Breast Cancer Bone Metastasis
    AARON DAVID MICKLE; Fiscal Year: 2013
    ..Metastatic breast cancer cells secrete high levels of parathyroid hormone-related peptide (PTHrP), which act on osteoblasts and osteoclasts to induce bone lysis/destruction and subsequent release of nutrients and cell signaling ..
  52. The Insulin/IGF-I Axis in Diabetic Osteopathy
    John L Fowlkes; Fiscal Year: 2013
    ..We also will study mice generated in our laboratory with conditional knock-out of the insulin receptor (IR) in osteoblasts (OIRKO mice) in regards to in utero skeletal development, postnatal affects on skeletal growth and development, ..
  53. Relationship between Hdac3 suppression and Wnt signaling in osteoblasts
    Meghan McGee-Lawrence; Fiscal Year: 2011
    ..Hdac3 is expressed in osteoblasts and binds to Runx2 to regulate osteoblastic gene expression, thus playing an important role in bone development ..
  54. Synthesis of bone-selective osteogenic oxysterols
    Frank Stappenbeck; Fiscal Year: 2013
    ..Multipotent mesenchymal stem cells (MSCs) are precursors of a variety of cell types, including osteoblasts and adipocytes...
  55. GFI-1 and Osteoblast Suppression in Multiple Myeloma
    GARSON DAVID ROODMAN; Fiscal Year: 2013
    ..the permanent MM-induced suppression of osteoblast precursor (OBP) differentiation into functional bone-forming osteoblasts. MM induces an intrinsic and persistent change in the OBP differentiation potential, the basis of which is ..
  56. ELUCIDATING THE FUNCTION OF MATRIPTASE-ACTIVATED PDGF D IN BONE METASTATIC PROSTA
    ABDO JAMAL NAJY; Fiscal Year: 2012
    ..of PDGF D may activate the tumor cell derived growth factor resulting in the activation of osteoclasts and osteoblasts leading to an aberrant osseous response...
  57. Role of Angiogenic CXC Chemokines in Intramembranous Bone Repair
    Dean T Yamaguchi; Fiscal Year: 2013
    ..to repair disrupted bone given the circumstances that mesenchymal stem cells (MSCs) can differentiate into osteoblasts (OBs) and that new blood supply can be re-established...
  58. Sp7 Mediated Control of Runx2 Function for Osteoblast Differentiation
    Amjad Javed; Fiscal Year: 2013
    ..The Sp7 deficient mesenchymal cells do not deposit bone matrix and cannot differentiate into osteoblasts. Very little is known about the underlying molecular mechanism for the surprisingly similar phenotype from the ..
  59. Conditional Isolation of Fgf23 Activity
    Kenneth E White; Fiscal Year: 2011
    ..FGF23 is expressed in osteoblasts and osteocytes, however the cell type(s) that 'sense'changes in serum phosphate to control production ..
  60. Pre-osteoclast fusion
    Paul R Odgren; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Bone resorption by osteoclasts (OCs) exceeding formation by osteoblasts (OBs) is a biological problem with a central role in widespread and costly disorders, including osteoporosis, osteo- and ..
  61. SINGLE SUTURE CRANIOSYNOSTOSIS: CANDIDATE GENE AND PATHWAY DISCOVERY
    Michael L Cunningham; Fiscal Year: 2013
    ..Specific Aim 1 will identify changes in the cellular phenotype of osteoblasts demonstrating activation of the IGF1-RUNX2 pathway...
  62. Mechanisms of adhesion and growth control in stem cells
    Reuben Kapur; Fiscal Year: 2010
    ..The BM microenvironment consists of extracellular matrix proteins as well as cells such as osteoblasts and stromal cells which contribute to the stem/progenitor cell fate by anchoring these cells in the BM cavity ..
  63. Imaging evidence: Is there competition between marrow fat and bone?
    Wei Shen; Fiscal Year: 2010
    ..Recent studies suggest that adipocytes in bone marrow and osteoblasts share the same precursor, mesenchymal stem cells (MSC). Human imaging studies [i.e...
  64. Mechanistic Role of NELL-1 in Premature Suture Closure
    Kang Ting; Fiscal Year: 2003
    ..Our preliminary data indicated preferential NELL-1 expression in neural crest origin cranial osteoblasts and during cranial suture closure...
  65. Mechanotransduction in Osteocytic Network and Osteoblast
    X Guo; Fiscal Year: 2007
    ..cells that are encased in mineralized extracellular bone matrix and interconnected with each other as well as osteoblasts through numerous intercellular processes by gap junctions...
  66. MAP KINASE REGULATION OF OSTEOBLAST FUNCTION
    Renny Franceschi; Fiscal Year: 2007
    unreadable] DESCRIPTION (provided by applicant): The transition from preosteoblasts to osteoblasts involves the secretion of a complex extracellular matrix containing bone morphogenetic proteins, growth factors and structural ..
  67. BASIC FIBROBLAST GROWTH FACTOR EXPRESSION IN BONE CELLS
    Marja Hurley; Fiscal Year: 2003
    Basic fibroblast growth factor (FGF-2), a potent mitogen for osteoblasts and marrow stromal cells, is synthesized by bone cells...
  68. STRUCTURE AND REGULATION OF OSTEOBLAST CALCIUM CHANNELS
    Elizabeth Barry; Fiscal Year: 2001
    ..of this research proposal is to understand how changes in the expression, or regulation of calcium channels in osteoblasts affect bone metabolism...
  69. Shaping the craniofacial skeleton in the zebrafish
    APRIL F DELAURIER; Fiscal Year: 2010
    The objective of my project is to understand how chondrocytes, which make cartilage, and osteoblasts, which make bone, generate the specific elements of the zebrafish facial skeleton...
  70. NEUROFIBROMIN (Nf1) SIGNALING AND TARGET GENES IN OSTEOBLASTS
    Florent Elefteriou; Fiscal Year: 2007
    ..and to overcome the embryonic lethality of Nf1-/- mice, we generated mice deficient for Nf1 specifically in osteoblasts (Nf1ob-/- mice)...
  71. TRANSCRIPTIONAL REGULATION BY FIBROBLAST GROWTH FACTOR
    Dwight Towler; Fiscal Year: 2001
    ..1 and -2 coordinates connective tissue remodeling during wound repair and contributes to the responses of osteoblasts to metastatic bone cancers...
  72. PROTEOMIC ANALYSIS OF OSTEOCYTES
    Norman Karin; Fiscal Year: 2009
    DESCRIPTION (provided by applicant): Osteocytes arise from osteoblasts that become surrounded by bone matrix and form a junctionally-connected sensory network that transduces physical strain into signals that maintain bone mass and ..
  73. LIF/IL6 INDUCED SIGNAL TRANSDUCTION IN BONE CELLS
    Mark Horowitz; Fiscal Year: 1999
    ..It is the application's hypothesis that members of this subfamily activate osteoblasts through specific receptors, resulting in tyrosine phosphorylation of a specific set of proteins...
  74. Parathyroid Hormone and Osteoblast Mitogenesis
    Louis Luttrell; Fiscal Year: 2005
    b>Osteoblasts regulate the deposition of bone matrix protein and its subsequent mineralization...