heart mitochondria

Summary

Summary: The mitochondria of the myocardium.

Top Publications

  1. pmc Supercomplexes in the respiratory chains of yeast and mammalian mitochondria
    H Schagger
    Zentrum der Biologischen Chemie, Universitatsklinikum Frankfurt, D 60590 Frankfurt, Germany
    EMBO J 19:1777-83. 2000
  2. ncbi Calpain inhibitor (BSF 409425) diminishes ischemia/reperfusion-induced damage of rabbit heart mitochondria
    Sonata Trumbeckaite
    Muskellabor der Neurologischen Klinik und Poliklinik der Martin Luther Universität Halle Wittenberg, Julius Kühn Str 7, D 06097 Halle Saale, Germany
    Biochem Pharmacol 65:911-6. 2003
  3. ncbi Mitochondrial permeability transition pore opening during myocardial reperfusion--a target for cardioprotection
    Andrew P Halestrap
    Department of Biochemistry and The Bristol Heart Institute, University of Bristol, Bristol BS8 1TD, UK
    Cardiovasc Res 61:372-85. 2004
  4. pmc Role of glycogen synthase kinase-3beta in cardioprotection
    Magdalena Juhaszova
    Laboratory of Cardiovascular Science, Gerontology Research Center, Box 13, National Institute on Aging, NIH, Baltimore, Maryland 21224 6825, USA
    Circ Res 104:1240-52. 2009
  5. pmc Energetic performance is improved by specific activation of K+ fluxes through K(Ca) channels in heart mitochondria
    Miguel A Aon
    The Johns Hopkins University, School of Medicine, Institute of Molecular Cardiobiology, 720 Rutland Ave, 1060 Ross Bldg, Baltimore, MD 21205 2195, USA
    Biochim Biophys Acta 1797:71-80. 2010
  6. pmc The mechanism of superoxide production by NADH:ubiquinone oxidoreductase (complex I) from bovine heart mitochondria
    Lothar Kussmaul
    Medical Research Council Dunn Human Nutrition Unit, Wellcome Trust Medical Research Council Building, Hills Road, Cambridge CB2 2XY, United Kingdom
    Proc Natl Acad Sci U S A 103:7607-12. 2006
  7. ncbi Exercise training induces respiratory substrate-specific decrease in Ca2+-induced permeability transition pore opening in heart mitochondria
    Mariannick Marcil
    Départment de Kinesiology, Universite de Montreal, PO Box 6128 Centre Ville, Montreal, PQ, Canada H3C 3J7
    Am J Physiol Heart Circ Physiol 290:H1549-57. 2006
  8. ncbi Specific inhibition of the mitochondrial permeability transition prevents lethal reperfusion injury
    Laurent Argaud
    INSERM E 0226, Laboratoire de Physiologie Lyon Nord, Faculté de Médecine Lyon Nord, Universite Claude Bernard Lyon I, 8, avenue Rockefeller, 69373 Lyon Cedex 8, France
    J Mol Cell Cardiol 38:367-74. 2005
  9. ncbi Mitochondrial energetics in the heart in obesity-related diabetes: direct evidence for increased uncoupled respiration and activation of uncoupling proteins
    Sihem Boudina
    Division of Endocrinology, Metabolism, and Diabetes, Program in Human Molecular Biology and Genetics, University of Utah School of Medicine, Salt Lake City, Utah 84112, USA
    Diabetes 56:2457-66. 2007
  10. ncbi Heart mitochondria: gates of life and death
    Asa B Gustafsson
    BioScience Center San Diego State University 5500 Campanile Drive San Diego, CA 92182 4650, USA
    Cardiovasc Res 77:334-43. 2008

Detail Information

Publications282 found, 100 shown here

  1. pmc Supercomplexes in the respiratory chains of yeast and mammalian mitochondria
    H Schagger
    Zentrum der Biologischen Chemie, Universitatsklinikum Frankfurt, D 60590 Frankfurt, Germany
    EMBO J 19:1777-83. 2000
    ..A fraction of total bovine ATP synthase (complex V) was isolated in dimeric form, suggesting that a dimeric state is not limited to S.cerevisiae, but also exists in mammalian mitochondria...
  2. ncbi Calpain inhibitor (BSF 409425) diminishes ischemia/reperfusion-induced damage of rabbit heart mitochondria
    Sonata Trumbeckaite
    Muskellabor der Neurologischen Klinik und Poliklinik der Martin Luther Universität Halle Wittenberg, Julius Kühn Str 7, D 06097 Halle Saale, Germany
    Biochem Pharmacol 65:911-6. 2003
    ..of a new developed calpain inhibitor (CI) BSF 409425 on the ischemia/reperfusion-induced damage of rabbit heart mitochondria was investigated...
  3. ncbi Mitochondrial permeability transition pore opening during myocardial reperfusion--a target for cardioprotection
    Andrew P Halestrap
    Department of Biochemistry and The Bristol Heart Institute, University of Bristol, Bristol BS8 1TD, UK
    Cardiovasc Res 61:372-85. 2004
    ..However, only if subsequent MPTP closure occurs will ATP levels be maintained, ensuring that cell death continues down an apoptotic, rather than a necrotic, pathway...
  4. pmc Role of glycogen synthase kinase-3beta in cardioprotection
    Magdalena Juhaszova
    Laboratory of Cardiovascular Science, Gerontology Research Center, Box 13, National Institute on Aging, NIH, Baltimore, Maryland 21224 6825, USA
    Circ Res 104:1240-52. 2009
    ....
  5. pmc Energetic performance is improved by specific activation of K+ fluxes through K(Ca) channels in heart mitochondria
    Miguel A Aon
    The Johns Hopkins University, School of Medicine, Institute of Molecular Cardiobiology, 720 Rutland Ave, 1060 Ross Bldg, Baltimore, MD 21205 2195, USA
    Biochim Biophys Acta 1797:71-80. 2010
    ..by measuring K+ flux, membrane potential (DeltaPsi(m)), light scattering, and respiration in guinea pig heart mitochondria. K+ uptake and the influence of anions were assessed in mitochondria loaded with the K+ sensor PBFI by ..
  6. pmc The mechanism of superoxide production by NADH:ubiquinone oxidoreductase (complex I) from bovine heart mitochondria
    Lothar Kussmaul
    Medical Research Council Dunn Human Nutrition Unit, Wellcome Trust Medical Research Council Building, Hills Road, Cambridge CB2 2XY, United Kingdom
    Proc Natl Acad Sci U S A 103:7607-12. 2006
    ..we describe the kinetic and molecular mechanism of superoxide production by complex I isolated from bovine heart mitochondria and confirm that it produces predominantly superoxide, not hydrogen peroxide...
  7. ncbi Exercise training induces respiratory substrate-specific decrease in Ca2+-induced permeability transition pore opening in heart mitochondria
    Mariannick Marcil
    Départment de Kinesiology, Universite de Montreal, PO Box 6128 Centre Ville, Montreal, PQ, Canada H3C 3J7
    Am J Physiol Heart Circ Physiol 290:H1549-57. 2006
    ..to determine whether regular exercise (treadmill running, 10 wk) alters the susceptibility of rat isolated heart mitochondria to Ca(2+)-induced permeability transition pore (PTP) opening and whether this could be associated with ..
  8. ncbi Specific inhibition of the mitochondrial permeability transition prevents lethal reperfusion injury
    Laurent Argaud
    INSERM E 0226, Laboratoire de Physiologie Lyon Nord, Faculté de Médecine Lyon Nord, Universite Claude Bernard Lyon I, 8, avenue Rockefeller, 69373 Lyon Cedex 8, France
    J Mol Cell Cardiol 38:367-74. 2005
    ..These data indicate that specific inhibition of MPT pore opening at reperfusion following acute myocardial infarction provides a powerful antinecrotic and antiapoptotic protection...
  9. ncbi Mitochondrial energetics in the heart in obesity-related diabetes: direct evidence for increased uncoupled respiration and activation of uncoupling proteins
    Sihem Boudina
    Division of Endocrinology, Metabolism, and Diabetes, Program in Human Molecular Biology and Genetics, University of Utah School of Medicine, Salt Lake City, Utah 84112, USA
    Diabetes 56:2457-66. 2007
    ..Mitochondrial uncoupling has been proposed to contribute to these metabolic abnormalities but has not been directly demonstrated...
  10. ncbi Heart mitochondria: gates of life and death
    Asa B Gustafsson
    BioScience Center San Diego State University 5500 Campanile Drive San Diego, CA 92182 4650, USA
    Cardiovasc Res 77:334-43. 2008
    ..In this review, we discuss the role of mitochondria in the control of cell death in cardiac myocytes...
  11. ncbi Mitochondrial DNA mutations, oxidative stress, and apoptosis in mammalian aging
    G C Kujoth
    Departments of Genetics and Medical Genetics, University of Wisconsin, Madison, WI 53706, USA
    Science 309:481-4. 2005
    ..The levels of apoptotic markers were also found to increase during aging in normal mice. Thus, accumulation of mtDNA mutations that promote apoptosis may be a central mechanism driving mammalian aging...
  12. pmc Peroxisome proliferator-activated receptor gamma coactivator-1 promotes cardiac mitochondrial biogenesis
    J J Lehman
    Department of Medicine, and Department of Pathology, Center for Cardiovascular Research, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Clin Invest 106:847-56. 2000
    ..These results identify PGC-1 as a critical regulatory molecule in the control of cardiac mitochondrial number and function in response to energy demands...
  13. ncbi Myocardial preconditioning against ischemia-reperfusion injury is abolished in Zucker obese rats with insulin resistance
    Prasad V G Katakam
    Department of Physiology and Pharmacology, Wake Forest University Health Sciences, Hanes 1050, Medical Center Blvd, Winston Salem, NC 27157, USA
    Am J Physiol Regul Integr Comp Physiol 292:R920-6. 2007
    ..Thus obesity accompanied by IR is associated with the inability to precondition against ischemic cardiac injury, which is mediated by enhanced mitochondrial oxidative stress and impaired activation of mitochondrial K(ATP)...
  14. ncbi Structure at 2.8 A resolution of F1-ATPase from bovine heart mitochondria
    J P Abrahams
    Medical Research Council Laboratory of Molecular Biology, Cambridge, UK
    Nature 370:621-8. 1994
    ..Interconversion of the states may be achieved by rotation of the alpha 3 beta 3 subassembly relative to an alpha-helical domain of the gamma-subunit...
  15. ncbi Changes in mitochondrial dynamics during ceramide-induced cardiomyocyte early apoptosis
    Valentina Parra
    Centro FONDAP Estudios Moleculares de la Celula, Universidad de Chile, Olivos 1007, Santiago 8380492, Chile
    Cardiovasc Res 77:387-97. 2008
    ..This process is associated with cell death. We investigated whether activation of apoptosis with ceramides affects mitochondrial dynamics and promotes mitochondrial fission in cardiomyocytes...
  16. ncbi Decrease in mitochondrial complex I activity in ischemic/reperfused rat heart: involvement of reactive oxygen species and cardiolipin
    Giuseppe Paradies
    Department of Biochemistry and Molecular Biology and CNR Institute of Biomembranes and Bioenergetics, University of Bari, Bari, Italy
    Circ Res 94:53-9. 2004
    ..These findings may provide an explanation for some of the factors responsible for myocardial reperfusion injury...
  17. ncbi Fo membrane domain of ATP synthase from bovine heart mitochondria: purification, subunit composition, and reconstitution with F1-ATPase
    I R Collinson
    MRC Laboratory of Molecular Biology, Cambridge, U K
    Biochemistry 33:7971-8. 1994
    The Fo membrane domain of the F1Fo-ATP synthase complex has been purified from bovine heart mitochondria. The purification procedure involves the removal of peripheral membrane proteins, including F1-ATPase, from submitochondrial ..
  18. pmc Cardioprotective signaling to mitochondria
    Keith D Garlid
    Department of Biology, Portland State University, Portland, OR 97201 0751, USA
    J Mol Cell Cardiol 46:858-66. 2009
    ..We review the experimental findings that bear on these hypotheses and other modes of protection involving mitochondria...
  19. ncbi Analysis of the subunit composition of complex I from bovine heart mitochondria
    Joe Carroll
    Medical Research Council Dunn Human Nutrition Unit, The Wellcome Trust MRC Building, Hills Road, Cambridge CB2 2XY, United Kingdom
    Mol Cell Proteomics 2:117-26. 2003
    Complex I purified from bovine heart mitochondria is a multisubunit membrane-bound assembly. In the past, seven of its subunits were shown to be products of the mitochondrial genome, and 35 nuclear encoded subunits were identified...
  20. ncbi Connexin 43 in cardiomyocyte mitochondria and its increase by ischemic preconditioning
    Kerstin Boengler
    Institut fur Pathophysiologie, Zentrum fur Innere Medizin, Universitatsklinikum Essen, Hufelandstr 55, 45122 Essen, Germany
    Cardiovasc Res 67:234-44. 2005
    ..Since mitochondria have been proposed to be involved in IP's protection, the present study analyzed whether Cx43 is localized at mitochondria of cardiomyocytes and whether such localization is affected by IP...
  21. pmc Biochemical dysfunction in heart mitochondria exposed to ischaemia and reperfusion
    Giancarlo Solaini
    Scuola Superiore di Studi Universitari e di Perfezionamento S Anna, Classe Accademica di Scienze Sperimentali, Piazza dei Martiri della Libertà 33, 56127 Pisa, Italy
    Biochem J 390:377-94. 2005
    ..Finally, we summarize the metabolic and pharmacological interventions that have been used to alleviate the effects of ischaemic injury, highlighting the value of these or related interventions in possible therapeutics...
  22. ncbi Ischemic defects in the electron transport chain increase the production of reactive oxygen species from isolated rat heart mitochondria
    Qun Chen
    Department of Medicine, School of Medicine, Case Western Reserve University, Cleveland, Ohio, USA
    Am J Physiol Cell Physiol 294:C460-6. 2008
    ..Thus ischemic damage to the ETC increased both the capacity and the net production of H(2)O(2) from complex I and complex III and sets the stage for an increase in ROS production during reperfusion as a mechanism of cardiac injury...
  23. ncbi Molecular and cellular basis of the aetiology and management of diabetic cardiomyopathy: a short review
    Ernest Adeghate
    Department of Anatomy, Faculty of Medicine and Health Sciences, United Arab Emirates University, Al Ain, United Arab Emirates
    Mol Cell Biochem 261:187-91. 2004
    ....
  24. ncbi Compensated volume overload increases the vulnerability of heart mitochondria without affecting their functions in the absence of stress
    Mariannick Marcil
    Département de Kinésiologie, Universite de Montreal, C P 6128, Succursale Centre Ville, Montreal, Quebec, Canada H3C 3J7
    J Mol Cell Cardiol 41:998-1009. 2006
    ....
  25. ncbi Mitochondrial uncoupling, with low concentration FCCP, induces ROS-dependent cardioprotection independent of KATP channel activation
    Jonathan P Brennan
    Cardiac Physiology Cardiovascular Division, The Rayne Institute, St Thomas Hospital, King s College London, SE1 7EH, UK
    Cardiovasc Res 72:313-21. 2006
    ....
  26. doi Increased susceptibility to Ca(2+)-induced permeability transition and to cytochrome c release in rat heart mitochondria with aging: effect of melatonin
    Giuseppe Petrosillo
    Department of Biochemistry and Molecular Biology, CNR Institute of Biomembranes and Bioenergetics, University of Bari, Italy
    J Pineal Res 48:340-6. 2010
    ..natural antioxidant, on the susceptibility to Ca(2+)-induced MPT opening and cytochrome c release in rat heart mitochondria. The mitochondrial content of normal and oxidized cardiolipin as a function of aging and melatonin treatment ..
  27. ncbi Mitochondrial K(ATP) channels: role in cardioprotection
    Olaf Oldenburg
    Department of Physiology, MSB 3024, University of South Alabama, College of Medicine, Mobile 36688, USA
    Cardiovasc Res 55:429-37. 2002
    ..Thus the mK(ATP) probably serves a dual role as both a trigger and a mediator. Possible end-effectors of preconditioning's protection are discussed including the mK(ATP) itself...
  28. ncbi Failure to precondition pathological human myocardium
    S Ghosh
    Department of Surgery, University Hospitals Leicester, United Kingdom
    J Am Coll Cardiol 37:711-8. 2001
    ....
  29. ncbi The direct physiological effects of mitoK(ATP) opening on heart mitochondria
    Alexandre D T Costa
    Dept of Biology, Portland State Univ, PO Box 751, Portland, OR 97207, USA
    Am J Physiol Heart Circ Physiol 290:H406-15. 2006
    ..Here, we examine the evidence for these hypotheses through experiments on isolated rat heart mitochondria. Using perturbation techniques, we show that matrix volume is the consequence of a steady-state balance ..
  30. ncbi Determination of the oxidation states of manganese in brain, liver, and heart mitochondria
    Thomas E Gunter
    Department of Biochemistry and Biophysics, University of Rochester, School of Medicine and Dentistry, Rochester, New York 14642, USA
    J Neurochem 88:266-80. 2004
    ..While the presence of Mn3+ complexes cannot be proven in the spectrum of endogenous mitochondrial manganese, the shape of this spectrum could suggest the presence of Mn3+ near the limit of detection, probably as MnSOD...
  31. pmc Aconitase and ATP synthase are targets of malondialdehyde modification and undergo an age-related decrease in activity in mouse heart mitochondria
    Connie S Yarian
    Department of Molecular Pharmacology and Toxicology, University of Southern California, 1985 Zonal Avenue, Los Angeles, CA 90033, USA
    Biochem Biophys Res Commun 330:151-6. 2005
    ..The biological consequences of an age-related decrease in aconitase and ATP synthase activities may contribute to the decline in mitochondrial bioenergetics evident during aging...
  32. ncbi Identification of a ryanodine receptor in rat heart mitochondria
    G Beutner
    Department of Pharmacology and Physiology, University of Rochester, School of Medicine and Dentistry, Rochester, New York 14642, USA
    J Biol Chem 276:21482-8. 2001
    ..In the presence of ryanodine, Ca(2+) uptake into isolated heart mitochondria is suppressed. In addition, ryanodine inhibited mitochondrial swelling induced by Ca(2+) overload...
  33. ncbi Role of the mitochondrial permeability transition in myocardial disease
    James N Weiss
    Cardiovascular Research Laboratory, Department of Medicine Cardiology, David Geffen School of Medicine at UCLA, Los Angeles, Calif 90095 1760, USA
    Circ Res 93:292-301. 2003
    ..Investigations into this area are beginning to unravel some of the mechanistic links between cardioprotective signaling and mitochondria...
  34. ncbi Hyperglycemia-induced apoptosis in mouse myocardium: mitochondrial cytochrome C-mediated caspase-3 activation pathway
    Lu Cai
    Department of Medicine, University of Louisville, Louisville, Kentucky, USA
    Diabetes 51:1938-48. 2002
    ..Hyperglycemia-induced myocardial apoptosis is mediated, at least in part, by activation of the cytochrome c-activated caspase-3 pathway, which may be triggered by ROS derived from high levels of glucose...
  35. ncbi The phosphorylation of subunits of complex I from bovine heart mitochondria
    Ruming Chen
    Medical Research Council Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 2XY, UK
    J Biol Chem 279:26036-45. 2004
    In bovine heart mitochondria and in submitochondrial particles, membrane-associated proteins with apparent molecular masses of 18 and 10 kDa become strongly radiolabeled by [(32)P]ATP in a cAMP-dependent manner...
  36. ncbi The flavoprotein subcomplex of complex I (NADH:ubiquinone oxidoreductase) from bovine heart mitochondria: insights into the mechanisms of NADH oxidation and NAD+ reduction from protein film voltammetry
    Chérise D Barker
    Medical Research Council Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 2XY, UK
    Biochemistry 46:3454-64. 2007
    Complex I (NADH:ubiquinone oxidoreductase) from bovine heart mitochondria contains 45 different subunits and nine redox cofactors...
  37. doi Effect of the multitargeted tyrosine kinase inhibitors imatinib, dasatinib, sunitinib, and sorafenib on mitochondrial function in isolated rat heart mitochondria and H9c2 cells
    Yvonne Will
    Exploratory Safety Differentiation, Pfizer, Inc, Groton, Connecticut 06340, USA
    Toxicol Sci 106:153-61. 2008
    ..Furthermore, we measured respiratory capacity of isolated rat heart mitochondria in the presence of the four kinase inhibitors and examined their effect on each of the oxidative ..
  38. ncbi Low rates of hydrogen peroxide production by isolated heart mitochondria associate with long maximum lifespan in vertebrate homeotherms
    Adrian J Lambert
    Medical Research Council, Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 2XY, UK
    Aging Cell 6:607-18. 2007
    ..We investigated this correlation by comparing rates of hydrogen peroxide (H(2)O(2)) production by heart mitochondria isolated from groups or pairs of species selected to have very different maximum lifespans but similar body ..
  39. ncbi Effects of desmin gene knockout on mice heart mitochondria
    M Linden
    Groupe de Biologie des Interactions Cellulaires, UMR CNRS 6558, Poitiers, France
    J Bioenerg Biomembr 33:333-41. 2001
    ..1997). In the present study we have isolated heart mitochondria from desmin null (D-/-) and control (D+/+) mice, and analyzed their composition by SDS-PAGE, immunoblotting, ..
  40. pmc Evidence for mitochondrial K+ channels and their role in cardioprotection
    BRIAN O'ROURKE
    Institute of Molecular Cardiobiology, Johns Hopkins University, 720 Rutland Ave, 844 Ross Bldg, Baltimore, MD 21205 2195, USA
    Circ Res 94:420-32. 2004
    ..This review updates recent progress in understanding the physiological role of mitoK(ATP) and highlights outstanding questions and controversies, with the intent of stimulating additional investigation on this topic...
  41. ncbi Inhibiting mitochondrial permeability transition pore opening at reperfusion protects against ischaemia-reperfusion injury
    Derek J Hausenloy
    The Hatter Institute and Centre for Cardiology, University College London Hospitals and Medical School, Grafton Way, WC1E 6DB London, UK
    Cardiovasc Res 60:617-25. 2003
    ..We hypothesised that the novel immunosuppressant, sanglifehrin-A (SFA), given at the time of reperfusion, protects the myocardium from ischaemia-reperfusion injury, by suppressing mPTP opening...
  42. pmc Bax translocates to mitochondria of heart cells during simulated ischaemia: involvement of AMP-activated and p38 mitogen-activated protein kinases
    Michela Capano
    Department of Biochemistry and Molecular Biology, University College London, Gower Street, London WC1E 6BT, UK
    Biochem J 395:57-64. 2006
    ..It is concluded that Bax translocation to mitochondria is an early step in ischaemia and that it occurs in response to activation of p38 MAPK downstream of AMPK...
  43. ncbi Sanglifehrin A acts as a potent inhibitor of the mitochondrial permeability transition and reperfusion injury of the heart by binding to cyclophilin-D at a different site from cyclosporin A
    Samantha J Clarke
    Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, United Kingdom
    J Biol Chem 277:34793-9. 2002
    ..Because SfA does not inhibit calcineurin activity, our data suggest that it may be more desirable than CsA for protecting tissues recovering from ischemic episodes and for studying the role of the MPTP in cell death...
  44. ncbi Diabetic cardiomyopathy revisited
    Sihem Boudina
    Division of Endocrinology, Metabolism and Diabetes and Program in Human Molecular Biology and Genetics, University of Utah School of Medicine, Salt Lake City 84112, USA
    Circulation 115:3213-23. 2007
    ..This review discusses the latest findings in diabetic humans and in animal models and reviews emerging new mechanisms that may be involved in the development and progression of cardiac dysfunction in diabetes...
  45. ncbi Overexpression of glutaredoxin-2 reduces myocardial cell death by preventing both apoptosis and necrosis
    Norbert Nagy
    Cardiovascular Research Center, University of Connecticut, School of Medicine, Farmington, CT 06030 1110, USA
    J Mol Cell Cardiol 44:252-60. 2008
    ..The results indicated a crucial role of mitochondrial Glrx2 in cardioprotection...
  46. ncbi Mitochondria are targets for geranylgeranylacetone-induced cardioprotection against ischemia-reperfusion in the rat heart
    Tetsuji Shinohara
    Department of Internal Medicine 1, Faculty of Medicine, Oita University, 1 1 Idaigaoka, Hasama, Oita 879 5593, Japan
    Am J Physiol Heart Circ Physiol 293:H1892-9. 2007
    ..These processes may involve opening of the mitoK(ATP) channel...
  47. ncbi Pharmacological and physiological stimuli do not promote Ca(2+)-sensitive K+ channel activity in isolated heart mitochondria
    Douglas V Cancherini
    Departamento de Bioquimica, Instituto de Quimica, Universidade de Sao Paulo, Sao Paulo, SP, Brazil
    Cardiovasc Res 73:720-8. 2007
    ..NS-1619 is cardioprotective, leading to the assumption that this effect is related to the opening of mitoK(Ca) channels. Here, we show several weaknesses in this hypothesis...
  48. ncbi Cardiac Fas-dependent and mitochondria-dependent apoptosis in ovariectomized rats
    Shin Da Lee
    Department of Physical Therapy and Graduate Institute of Rehabilitation Science, China Medical University, Taichung, Taiwan
    Maturitas 61:268-77. 2008
    ..The purpose of this study was to evaluate whether cardiac Fas-dependent (type I) and mitochondria-dependent (type II) apoptotic pathways are activated in ovariectomized rats...
  49. ncbi Reversible blockade of electron transport during ischemia protects mitochondria and decreases myocardial injury following reperfusion
    Qun Chen
    Department of Medicine, Division of Cardiology, School of Medicine, Case Western Reserve University, Cleveland, Ohio, USA
    J Pharmacol Exp Ther 319:1405-12. 2006
    ..Reperfusion in the setting of preserved mitochondrial respiratory function attenuates the mitochondrial release of reactive oxygen species, enhances contractile recovery, and decreases myocardial infarct size...
  50. pmc Investigation of the mechanism of proton translocation by NADH:ubiquinone oxidoreductase (complex I) from bovine heart mitochondria: does the enzyme operate by a Q-cycle mechanism?
    Steven Sherwood
    Medical Research Council Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 2XY, UK
    Biochem J 400:541-50. 2006
    ..Here, we describe experiments to determine whether complex I, isolated from bovine heart mitochondria, operates via a Q-cycle mechanism analogous to that observed in the cytochrome bc1 complex...
  51. doi Role of apoptosis in cardiovascular disease
    Youngil Lee
    BioScience Center, San Diego State University, San Diego, CA 92182 4650, USA
    Apoptosis 14:536-48. 2009
    ..This review provides a current overview of the evidence of apoptosis in cardiovascular diseases and discusses the molecular pathways involved in cardiac myocyte apoptosis...
  52. ncbi Release of cytochrome c from heart mitochondria is induced by high Ca2+ and peroxynitrite and is responsible for Ca(2+)-induced inhibition of substrate oxidation
    V Borutaite
    Institute for Biomedical Research, Kaunas Medical University, Lithuania
    Biochim Biophys Acta 1453:41-8. 1999
    ..and other inducers of mitochondrial permeability transition cause the release of cytochrome c from isolated heart mitochondria. We found that 5 microM free Ca2+ induced changes in oxidative phosphorylation system similar to ischaemic ..
  53. pmc Ischaemic preconditioning inhibits opening of mitochondrial permeability transition pores in the reperfused rat heart
    Sabzali A Javadov
    Department of Biochemistry, University of Bristol, Bristol BS8 1TD, UK
    J Physiol 549:513-24. 2003
    ..Our data suggest that protection from reperfusion injury is better achieved by reducing factors that induce MPTP opening than by inhibiting the MPTP directly...
  54. ncbi Effects of NO on mitochondrial function in cardiomyocytes: Pathophysiological relevance
    Sean M Davidson
    The Hatter Cardiovascular Institute, Department of Medicine, Royal Free and University College Medical School, 67 Chenies Mews, University College Hospital, London WC1E 6HX, United Kingdom
    Cardiovasc Res 71:10-21. 2006
    ....
  55. ncbi Low complex I content explains the low hydrogen peroxide production rate of heart mitochondria from the long-lived pigeon, Columba livia
    Adrian J Lambert
    Medical Research Council Mitochondrial Biology Unit, Hills Road, Cambridge, CB2 0XY, UK
    Aging Cell 9:78-91. 2010
    ..this report, we investigate the underlying biochemical basis of the low hydrogen peroxide production rate of heart mitochondria from a long-lived species (pigeon) compared with a short-lived species with similar body mass (rat)...
  56. doi The nitrated proteome in heart mitochondria of the db/db mouse model: characterization of nitrated tyrosine residues in SCOT
    Yuan Wang
    Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, China
    J Proteome Res 9:4254-63. 2010
    ..2DE, Western blot, and mass spectrometry was implemented to survey the status of tyrosine nitration in mouse heart mitochondria. Compared to normal mice, nitrated proteins in the heart mitochondria of the db/db mouse model were ..
  57. ncbi Cardioprotective effect of diazoxide is mediated by activation of sarcolemmal but not mitochondrial ATP-sensitive potassium channels in mice
    Masashi Suzuki
    Department of Pharmacology, Graduate School of Medicine, Chiba University, Chiba, Japan
    Circulation 107:682-5. 2003
    ..2-knockout (KO) mice. In the present study, we evaluated the effects of diazoxide, a mitochondrial ATP-sensitive potassium (mitoK(ATP)) channel opener, on ischemia-induced myocardial stunning in sarcK(ATP) channel-deficient mice...
  58. ncbi Heart mitochondria contain functional ATP-dependent K+ channels
    Zsombor Lacza
    Department of Physiology and Pharmacology, Wake Forest University School of Medicine, Winston Salem, NC 27157, USA
    J Mol Cell Cardiol 35:1339-47. 2003
    ..In the present study, we determined the presence of K(ATP)-channel subunits in mouse heart mitochondria, and investigated whether known openers or blockers of the channel can alter mitochondrial membrane ..
  59. pmc The effects of ischaemic preconditioning, diazoxide and 5-hydroxydecanoate on rat heart mitochondrial volume and respiration
    Kelvin H H Lim
    The Bristol Heart Institute, Bristol Royal Infirmary, Malborough Street, Bristol BS2 8HW, UK
    J Physiol 545:961-74. 2002
    ..These data highlight the dangers of using 5HD and diazoxide as specific modulators of mitoK(ATP) channels in the heart...
  60. ncbi Thiamine ameliorates diabetes-induced inhibition of pyruvate dehydrogenase (PDH) in rat heart mitochondria: investigating the discrepancy between PDH activity and PDH E1alpha phosphorylation in cardiac fibroblasts exposed to high glucose
    Yuka Kohda
    Laboratory of Pharmacotherapy, Osaka University of Pharmaceutical Sciences, Takatsuki, Japan
    J Pharmacol Sci 113:343-52. 2010
    ..The O-glycosylation of PDH E1alpha may be involved in the regulation of the PDH activity...
  61. ncbi Endurance training limits the functional alterations of rat heart mitochondria submitted to in vitro anoxia-reoxygenation
    Antonio Ascensao
    Department of Sport Biology, Faculty of Sport Sciences, University of Porto, Portugal
    Int J Cardiol 109:169-78. 2006
    ..The present study aimed to investigate the effect of moderate endurance treadmill training (14 weeks) against rat heart mitochondrial dysfunction induced by in vitro A-R...
  62. ncbi Modulation of the mitochondrial permeability transition pore complex in GSK-3beta-mediated myocardial protection
    Masahiro Nishihara
    Second Department of Internal Medicine, Sapporo Medical University School of Medicine, S 1 W 16, Chuo Ku, Sapporo 060 8543, Japan
    J Mol Cell Cardiol 43:564-70. 2007
    ..These results suggest that reduction in affinity of ANT to cyclophilin D by increased phospho-GSK-3beta binding to ANT may be responsible for suppression of mPTP opening and myocardial protection afforded by IPC+EPO...
  63. ncbi Real-time 2-photon imaging of mitochondrial function in perfused rat hearts subjected to ischemia/reperfusion
    Madoka Matsumoto-Ida
    Department of Cardiovascular Medicine, Kyoto University Graduate School of Medicine, 54 Kawahara cho, Shogoin, Sakyo ku, Kyoto 606 8507, Japan
    Circulation 114:1497-503. 2006
    ....
  64. ncbi Complementary DNA sequences of two 14.5 kDa subunits of NADH:ubiquinone oxidoreductase from bovine heart mitochondria. Completion of the primary structure of the complex?
    J M Arizmendi
    Medical Research Council Laboratory of Molecular Biology, Cambridge, UK
    FEBS Lett 313:80-4. 1992
    The amino acid sequences of two nuclear-encoded subunits of complex I from bovine heart mitochondria have been determined. Both proteins have an apparent molecular weight of 14.5 kDa and their N-alpha-amino groups are acetylated...
  65. ncbi Blockade of electron transport before cardiac ischemia with the reversible inhibitor amobarbital protects rat heart mitochondria
    Qun Chen
    Dept of Medicine, Cardiology Section, Medical Service 111 W, Case Western Reserve University, Louis Stokes Veterans Affairs Medical Center, 10701 East Boulevard, Cleveland, OH 44106, USA
    J Pharmacol Exp Ther 316:200-7. 2006
    ..5 mM. Amobarbital also preserved cytochrome c content in both SSM and IFM. Thus, reversible blockade of the electron transport chain during ischemia protects mitochondrial respiration...
  66. ncbi Arachidonic acid induces specific membrane permeability increase in heart mitochondria
    Marco Di Paola
    Institute of Biomembranes and Bioenergetics Consiglio Nazionale delle Ricerche Bari, Italy
    FEBS Lett 580:775-81. 2006
    Micromolar concentrations of arachidonic acid cause in Ca2+ loaded heart mitochondria matrix swelling and Ca2+ release...
  67. ncbi NHE-1 inhibition improves impaired mitochondrial permeability transition and respiratory function during postinfarction remodelling in the rat
    Sabzali Javadov
    Department Physiology and Pharmacology, University of Western Ontario, London, Ont, Canada
    J Mol Cell Cardiol 38:135-43. 2005
    ..We conclude that NHE-1 inhibition has a protective effect on mitochondrial function, attenuating MPT pore opening and improving the respiratory function, which may contribute to the salutary effect of NHE-1 inhibitors in heart failure...
  68. ncbi Bioenergetic remodeling of heart mitochondria by thyroid hormone
    Michael J Goldenthal
    The Molecular Cardiology and Neuromuscular Institute, Highland Park, NJ 08904, USA
    Mol Cell Biochem 265:97-106. 2004
    ..The marked, parallel increases in PPARalpha levels suggest its potential involvement in mediating myocardial-specific remodeling of mitochondria in response to T4...
  69. pmc 5-Hydroxydecanoate is metabolised in mitochondria and creates a rate-limiting bottleneck for beta-oxidation of fatty acids
    Peter J Hanley
    Institut fur Normale und Pathologische Physiologie, Universitat Marburg, Deutschhausstrasse 2, 35037 Marburg, Germany
    J Physiol 562:307-18. 2005
    ..using specially synthesised (and purified) substrates and enzymes, as well as isolated rat liver and heart mitochondria, and compared it with the metabolism of the physiological substrate decanoyl-CoA...
  70. ncbi Bovine complex I is a complex of 45 different subunits
    Joe Carroll
    Dunn Human Nutrition Unit, The Medical Research Council, Hills Road, Cambridge CB2 2XY, United Kingdom
    J Biol Chem 281:32724-7. 2006
    ..Thus, the subunit composition of bovine complex I has been established. It is a complex of 45 different proteins plus non-covalently bound FMN and eight iron-sulfur clusters...
  71. ncbi FCCP is cardioprotective at concentrations that cause mitochondrial oxidation without detectable depolarisation
    Jonathan P Brennan
    Cardiac Physiology Cardiovascular Division, King s College London, SE1 7EH, UK
    Cardiovasc Res 72:322-30. 2006
    ..Here we define the mitochondrial responses to increasing concentrations of FCCP and also to explore the equivalence of the cardioprotective doses of diazoxide...
  72. ncbi Inhibition of phosphate transport in rat heart mitochondria by 3'-azido-3'-deoxythymidine due to stimulation of superoxide anion mitochondrial production
    Daniela Valenti
    Centro di Studio sui Mitocondri e Metabolismo Energetico, C N R, Via Amendola 165, 70126 Bari, Italy
    Biochem Pharmacol 64:201-6. 2002
    ..we investigated whether externally added AZT can stimulate reactive oxygen species (ROS) production by rat heart mitochondria (RHM). An increase in superoxide anion ((O(2)(...
  73. ncbi Voltammetric studies of bidirectional catalytic electron transport in Escherichia coli succinate dehydrogenase: comparison with the enzyme from beef heart mitochondria
    H R Pershad
    Inorganic Chemistry Laboratory, University of Oxford, South Parks Road, Oxford, UK
    Biochim Biophys Acta 1412:262-72. 1999
    ..soluble, membrane-extrinsic subunits of succinate:quinone oxidoreductases) from Escherichia coli and beef heart mitochondria each adsorb at a pyrolytic graphite 'edge' electrode and catalyse the interconversion of succinate and ..
  74. ncbi Nitric oxide synthase in porcine heart mitochondria: evidence for low physiological activity
    S French
    Laboratory of Cardiac Energetics, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Am J Physiol Heart Circ Physiol 280:H2863-7. 2001
    The capacity of isolated porcine heart mitochondria to produce nitric oxide (NO) via mitochondrial NO synthase (NOS) was evaluated. The mitochondrial NOS content and activity (0...
  75. ncbi Mitochondrial K(ATP) channel opening protects a human atrial-derived cell line by a mechanism involving free radical generation
    R Carroll
    The Hatter Institute and Centre for Cardiology, University College Hospital and Medical School, Grafton Way, London WC1E 6DB, UK
    Cardiovasc Res 51:691-700. 2001
    ....
  76. pmc A heart mitochondrial Ca2(+)-dependent pore of possible relevance to re-perfusion-induced injury. Evidence that ADP facilitates pore interconversion between the closed and open states
    M Crompton
    Department of Biochemistry, University College London, U K
    Biochem J 266:33-9. 1990
    The permeability properties of a putative Ca2(+)-activated pore in heart mitochondria, of possible relevance to re-perfusion-induced injury, have been investigated by a pulsed-flow solute-entrapment technique...
  77. ncbi Mitochondria: a target for myocardial protection
    M S Suleiman
    Bristol Heart Institute, University of Bristol, Bristol Royal Infirmary, Malborough Street, Bristol BS2 8HW, UK
    Pharmacol Ther 89:29-46. 2001
    ..Finally, we describe use of cardioplegic solutions in the clinical setting, and discuss how improved understanding of the aspects of mitochondrial function summarised above may lead to better protective strategies in the future...
  78. ncbi The exogenous NADH dehydrogenase of heart mitochondria is the key enzyme responsible for selective cardiotoxicity of anthracyclines
    H Nohl
    Institute of Pharmacology and Toxicology, Veterinary University Vienna, Austria
    Z Naturforsch C 53:279-85. 1998
    ..The results presented in this study demonstrate that, in contrast to liver mitochondria, isolated heart mitochondria shuttle single electrons to adriamycin, giving rise to oxygen radical formation via autoxidation of ..
  79. pmc NADH:ubiquinone oxidoreductase from bovine heart mitochondria. cDNA sequences of the import precursors of the nuclear-encoded 39 kDa and 42 kDa subunits
    I M Fearnley
    Medical Research Council Laboratory of Molecular Biology, Cambridge, U K
    Biochem J 278:821-9. 1991
    The 39 kDa and 42 kDa subunits of NADH:ubiquinone oxidoreductase from bovine heart mitochondria are nuclear-coded components of the hydrophobic protein fraction of the enzyme...
  80. ncbi Identification of the subunits of F1F0-ATPase from bovine heart mitochondria
    J E Walker
    Medical Research Council, Laboratory of Molecular Biology, Cambridge, U K
    Biochemistry 30:5369-78. 1991
    An oligomycin-sensitive F1F0-ATPase isolated from bovine heart mitochondria has been reconstituted into phospholipid vesicles and pumps protons...
  81. ncbi Carnitine palmitoyl transferase I and the control of beta-oxidation in heart mitochondria
    S Eaton
    Unit of Paediatric Surgery, Institute of Child Health, 30 Guilford Street, London, WC1N 1EH, United Kingdom
    Biochem Biophys Res Commun 285:537-9. 2001
    ..control coefficient of carnitine palmitoyl transferase I over beta-oxidation carbon flux in suckling rat heart mitochondria. The flux control coefficient was found to be 0.08 +/- 0...
  82. ncbi Effects of thyroid state on H2O2 production by rat heart mitochondria: sites of production with complex I- and complex II-linked substrates
    P Venditti
    Dipartimento di Fisiologia Generale ed Ambientale, Universita di Napoli, Italy
    Horm Metab Res 35:55-61. 2003
    ..to determine possible effects of altered thyroid states on rates and sites of H 2 O 2 production by rat heart mitochondria. Rates of O 2 consumption and H 2 O 2 release, capacities to remove the peroxide, lipid peroxidation, ..
  83. ncbi Protection of cardiac mitochondria by overexpression of MnSOD reduces diabetic cardiomyopathy
    Xia Shen
    Department of Pathology, University of Washington, Seattle, USA
    Diabetes 55:798-805. 2006
    ..These results showed that elevating MnSOD provided extensive protection to diabetic mitochondria and provided overall protection to the diabetic heart...
  84. ncbi Sodium tanshinone IIA sulfonate mediates electron transfer reaction in rat heart mitochondria
    Guangyin Zhou
    Laboratory of Visual Information Processing, Department of Molecular and Cell Biophysics, Institute of Biophysics, Academia Sinica, Beijing 100101, PR China
    Biochem Pharmacol 65:51-7. 2003
    ..paper, an electron transfer reaction mediated by sodium tanshinone IIA sulfonate (STS) was studied in rat heart mitochondria. It was found that STS could stimulate mitochondrial NADH oxidation dose-dependently and partly restore NADH ..
  85. ncbi Potassium-specific effects of levosimendan on heart mitochondria
    Dalia M Kopustinskiene
    Institute for Biomedical Research, Kaunas University of Medicine, Kaunas, Lithuania
    Biochem Pharmacol 68:807-12. 2004
    ..in mitochondrial inner membrane permeability for potassium by registering changes in membrane potential of heart mitochondria, oxidizing endogenous substrates...
  86. ncbi Dang-Gui Buxue Tang produces a more potent cardioprotective effect than its component herb extracts and enhances glutathione status in rat heart mitochondria and erythrocytes
    Duncan H F Mak
    Department of Biochemistry, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
    Phytother Res 20:561-7. 2006
    ..The measurement of RBC glutathione status may serve as a useful index for the antioxidant effect produced by DBT treatment in human subjects...
  87. ncbi Roles of mitochondrial ATP-sensitive K channels and PKC in anti-infarct tolerance afforded by adenosine A1 receptor activation
    T Miura
    Second Department of Internal Medicine, Sapporo Medical University School of Medicine, Japan
    J Am Coll Cardiol 35:238-45. 2000
    ..This study intended to assess the role of mitochondrial ATP-sensitive potassium (mitoK ATP) channels and the sequence of signal transduction with protein kinase C (PKC) and adenosine A1 receptors in rabbits...
  88. ncbi Regucalcin increases Ca2+-ATPase activity in the heart mitochondria of normal and regucalcin transgenic rats
    Tania Akhter
    Laboratory of Endocrinology and Molecular Metabolism, Graduate School of Nutritional Sciences, University of Shizuoka, Shizuoka 422 8526, Japan
    Int J Mol Med 18:171-6. 2006
    ..a regulatory protein in intracellular signaling system, in the regulation of Ca2+-ATPase activity in rat heart mitochondria was investigated...
  89. ncbi Generation of superoxide-radical by the NADH:ubiquinone oxidoreductase of heart mitochondria
    A D Vinogradov
    Department of Biochemistry, Faculty of Biology, Lomonosov Moscow State University, Moscow 119992, Russia
    Biochemistry (Mosc) 70:120-7. 2005
    ..One site (F) serves as an entry for the NADH oxidation and the other one (R) serves as an exit during either the succinate-supported NAD+ reduction or superoxide generation or NADH-ferricyanide reductase reaction...
  90. ncbi Seasonal variations in properties of healthy and diabetic rat heart mitochondria: Mg2+-ATPase activity, content of conjugated dienes and membrane fluidity
    J Mujkosová
    Institute for Heart Research, Slovak Academy of Sciences, Bratislava, Slovakia
    Physiol Res 57:S75-82. 2008
    Our previous preliminary results pointed to possible seasonal variations in Mg2+-ATPase activity of rat heart mitochondria (MIT)...
  91. ncbi The amino acid sequence of GTP:AMP phosphotransferase from beef-heart mitochondria. Extensive homology with cytosolic adenylate kinase
    B Wieland
    Eur J Biochem 143:331-9. 1984
    The amino acid sequence of GTP:AMP phosphotransferase (AK3) from beef-heart mitochondria has been determined, except for one segment of about 33 residues in the middle of the polypeptide chain...
  92. pmc Mn porphyrin-based superoxide dismutase (SOD) mimic, MnIIITE-2-PyP5+, targets mouse heart mitochondria
    Ivan Spasojevic
    Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA
    Free Radic Biol Med 42:1193-200. 2007
    ..55:39-46; 1979). We found MnIIITE-2-PyP5+ localized in heart mitochondria to 2.95 ng/mg protein. Given the average value of mitochondrial volume of 0...
  93. ncbi Attenuation by metallothionein of early cardiac cell death via suppression of mitochondrial oxidative stress results in a prevention of diabetic cardiomyopathy
    Lu Cai
    Department of Medicine, The University of Louisville, Louisville, Kentucky, USA
    J Am Coll Cardiol 48:1688-97. 2006
    ..We aimed to test whether attenuation of early-phase cardiac cell death can prevent diabetic cardiomyopathy...
  94. doi Postconditioning prevents reperfusion injury by activating delta-opioid receptors
    Youngho Jang
    Department of Anesthesiology, The University of North Carolina at Chapel Hill, North Carolina 27599, USA
    Anesthesiology 108:243-50. 2008
    ..The authors hypothesized that postconditioning stimulates opioid receptors, which in turn protect the heart from reperfusion injury by targeting the mPTP...
  95. ncbi Cardiac mitochondrial damage and biogenesis in a chronic model of type 1 diabetes
    Xia Shen
    Dept of Pharmacology and Toxicology, University of Louisville, 570 S Preston St, Suite 304, Louisville, KY 40202, USA
    Am J Physiol Endocrinol Metab 287:E896-905. 2004
    ..Taken together, these results show that mitochondria are a primary target in the diabetic heart, probably due to oxidative stress, and that this damage coincides with and may stimulate mitochondrial biogenesis...
  96. ncbi Mitochondria and ischemia-reperfusion injury of the heart: fixing a hole
    Fabio Di Lisa
    Dipartimento di Chimica Biologica, Universita di Padova, Viale G Colombo 3, 35121 Padova
    Cardiovasc Res 70:191-9. 2006
    ..Here, we discuss our current understanding of the structure and function of PTP in the context of heart injury caused by ischemia and reperfusion...
  97. ncbi Detection of reactive oxygen species-sensitive thiol proteins by redox difference gel electrophoresis: implications for mitochondrial redox signaling
    Thomas R Hurd
    Medical Research Council Dunn Human Nutrition Unit, Wellcome Trust Medical Research Council Building, Hills Road, Cambridge, UK
    J Biol Chem 282:22040-51. 2007
    ..Thiol redox modification affected enzyme activity, suggesting that the reversible modification of enzyme activity by ROS from the respiratory chain may be an important and unexplored mode of mitochondrial redox signaling...
  98. ncbi Postconditioning inhibits mitochondrial permeability transition
    Laurent Argaud
    INSERM E 0226, Universite Claude Bernard Lyon I, France
    Circulation 111:194-7. 2005
    ..quot; After reflow, opening of the mitochondrial permeability transition pore (mPTP) has been involved in lethal reperfusion injury. We hypothesized that postconditioning may modulate mPTP opening...
  99. pmc K(ATP) channel-independent targets of diazoxide and 5-hydroxydecanoate in the heart
    Peter J Hanley
    Institut fur Normale und Pathologische Physiologie, Universitat Marburg, Deutschhausstrasse 2, 35037 Marburg, Germany
    J Physiol 542:735-41. 2002
    ..We propose that pharmacological preconditioning may be related to partial inhibition of respiratory chain complexes...
  100. ncbi Mitochondrial potassium transport: the K(+) cycle
    Keith D Garlid
    Department of Biology, Portland State University, 1719 SW 10th Avenue, PO Box 751, Portland, OR 97207, USA
    Biochim Biophys Acta 1606:23-41. 2003
    ..This review discusses the properties of the mitochondrial K(+) cycle that help to understand the basis of these diverse effects...
  101. ncbi Mitochondrial PKCepsilon and MAPK form signaling modules in the murine heart: enhanced mitochondrial PKCepsilon-MAPK interactions and differential MAPK activation in PKCepsilon-induced cardioprotection
    Christopher P Baines
    Department of Physiology, University of Louisville, and the Jewish Hospital Heart and Lung Institute, Louisville, KY 40202, USA
    Circ Res 90:390-7. 2002
    ..Furthermore, formation of mitochondrial PKCepsilon-ERK modules appears to play a role in PKCepsilon-mediated cardioprotection, in part by the phosphorylation and inactivation of Bad...

Research Grants67

  1. COMPLEX III AUGMENTS OXIDANTS DAMAGE IN ISCHEMIC AGED HEART
    EDWARD LESNEFSKY JR; Fiscal Year: 2004
    ..We hypothesize that following ischemic damage to aged heart mitochondria, ROS production and release is increased, setting the stage for oxidative damage during reperfusion...
  2. MITOCHONDRIAL IRON-SULFUR PROTEINS
    Michael Johnson; Fiscal Year: 1990
    ..the functional, structural, electronic and magnetic properties of the multiple iron-sulfur centers in beef heart mitochondria. This will be accomplished by investigations of the membrane-bound enzymes of the mitochondrial electron ..
  3. BRANCHED CHAIN AMINO ACID METABOLISM IN SKELETAL MUSCLE
    SUSAN HUTSON; Fiscal Year: 1990
    ..The kinetic pattern for BCKA uptake will be determined in rat heart mitochondria. Inhibitor and substrate specificities of the BCKA and pyruvate transporters will be compared, and ..
  4. STUDIES OF O2 ACTIVATION BY MOSSBAUER SPECTROSCOPY
    Eckard Munck; Fiscal Year: 1990
    ..The a3-site of the enzyme, the locus of oxygen reduction, will be studied with enzymes isolated from beef heart mitochondria, Thermus thermophilus and Nitrosomonas europeae...
  5. Microxymetry Techniques for Cardiomyocyte Respiration
    Govindasamy Ilangovan; Fiscal Year: 2004
    ..production will also be studied using the proposed microxymetry; (ii) to study the respiration of isolated heart mitochondria using the proposed microxymtery...
  6. Microxymetry Techniques for Cardiomocyte Respiration
    Govindasamy Ilangovan; Fiscal Year: 2005
    ..production will also be studied using the proposed microxymetry; (ii) to study the respiration of isolated heart mitochondria using the proposed microxymtery...
  7. Cations and ROS in Modulating Mitochondrial Function in Normal and Ischemic Heart
    RANJAN K contact DASH; Fiscal Year: 2010
    ..g., cation channel blockers or openers) to alleviate mitochondrial dysfunction and preserve cellular viability. ..
  8. METABOLISM OF UNSATURATED AND HYDROXY FATTY ACIDS
    Horst Schulz; Fiscal Year: 1990
    ..between the rates of oxidation of cis and trans unsaturated fatty acids will be determined with rat heart mitochondria. The oxidation of hydroxy fatty acids including 5-hydroxy-6,8,11,13-eicosatetraenoic acid (5-HETE) in ..
  9. ELECTRON TRANSLOCATION IN BIOLOGICAL ENERGY CONVERSION
    P Dutton; Fiscal Year: 1990
    ..sphaeroides), cytochrome c and bc1 complexes, (beef heart mitochondria)...
  10. Heart mitochondrial toxicity of antiviral nucleosides
    Edward McKee; Fiscal Year: 2006
    ..the HAART components, AZT (Zidovudine) is converted slowly only to AZT-MP and no further, in both isolated heart mitochondria and in the isolated perfused rat heart under conditions in which labeled thymidine is readily converted to ..
  11. Heart mitochondrial toxicity of antiviral nucleosides
    Edward McKee; Fiscal Year: 2004
    ..the HAART components, AZT (Zidovudine) is converted slowly only to AZT-MP and no further, in both isolated heart mitochondria and in the isolated perfused rat heart under conditions in which labeled thymidine is readily converted to ..
  12. Heart mitochondrial toxicity of antiviral nucleosides
    Edward McKee; Fiscal Year: 2005
    ..the HAART components, AZT (Zidovudine) is converted slowly only to AZT-MP and no further, in both isolated heart mitochondria and in the isolated perfused rat heart under conditions in which labeled thymidine is readily converted to ..
  13. Heart mitochondrial toxicity of antiviral nucleosides
    Edward McKee; Fiscal Year: 2003
    ..the HAART components, AZT (Zidovudine) is converted slowly only to AZT-MP and no further, in both isolated heart mitochondria and in the isolated perfused rat heart under conditions in which labeled thymidine is readily converted to ..
  14. Heart mitochondrial toxicity of antiviral nucleosides
    Edward McKee; Fiscal Year: 2002
    ..the HAART components, AZT (Zidovudine) is converted slowly only to AZT-MP and no further, in both isolated heart mitochondria and in the isolated perfused rat heart under conditions in which labeled thymidine is readily converted to ..
  15. Heart mitochondrial toxicity of antiviral nucleosides
    Edward McKee; Fiscal Year: 2005
    ..the HAART components, AZT (Zidovudine) is converted slowly only to AZT-MP and no further, in both isolated heart mitochondria and in the isolated perfused rat heart under conditions in which labeled thymidine is readily converted to ..
  16. REGULATION OF LEUCINE OXIDATION
    Robert Harris; Fiscal Year: 1980
    ..Studies will be conducted with isolated hepatocytes, perfused rat heart, isolated liver and heart mitochondria, and branched-chain alpha-keto acid dehydrogenase complex isolated from liver or heart tissue...
  17. ENERGY TRANSDUCING ATPASE
    William Allison; Fiscal Year: 1990
    ..these goals, chemical modification studies are being conducted in parallel on the F1-ATPases from bovine heart mitochondria (MF1), the plasma membrane of E...
  18. PROTON & ELECTRON TRANSFER & ENERGY COUPLING IN SITE I
    Tomoko Ohnishi; Fiscal Year: 2007
    ..Complex I is the major site of superoxide generation in brain and heart mitochondria, but no consensus has been attained regarding the generation site(s)...
  19. PROTON & ELECTRON TRANSFER & ENERGY COUPLING IN SITE I
    Tomoko Ohnishi; Fiscal Year: 2009
    ..Complex I is the major site of superoxide generation in brain and heart mitochondria, but no consensus has been attained regarding the generation site(s)...
  20. PROTON & ELECTRON TRANSFER & ENERGY COUPLING IN SITE I
    TOMOKO NONE OHNISHI; Fiscal Year: 2010
    ..Complex I is the major site of superoxide generation in brain and heart mitochondria, but no consensus has been attained regarding the generation site(s)...
  21. ORGANIZATION OF SUCCINIC OXIDASE IN HEART
    Roderick Capaldi; Fiscal Year: 1980
    ..are directed at determining the arrangement of succinic oxidase, a major segment of the respiratory chain of heart mitochondria. Succinic oxidase as a unit has not been isolated but can be reconstituted from three purified and ..
  22. FATTY ACID METABOLISM IN HEART AND ITS REGULATIONS
    Horst Schulz; Fiscal Year: 1990
    ..of the intramitochondrial acetyl-CoA/CoASH ratio and the NADH/NAD+ ration on the rate of beta-oxidation in heart mitochondria will be evaluated...
  23. EFFECT OF DIET AND AGING ON MACROMOLECULAR SYNTHESIS
    Arlan Richardson; Fiscal Year: 1980
    ..the effect of dietary restriction on heart mitochondria is determined by measuring the numerical density of mitochondria from samples of heart tissue and the rate ..
  24. FATTY ACID OXIDATION IN ISCHEMIC/REPERFUSED AGING HEARTS
    Charles Hoppel; Fiscal Year: 2001
    ..At baseline, aging-defects in the mitochondrial electron transport chain occur in only one population of heart mitochondria (interfibrillar) in elderly Fischer 344 rats...
  25. FATTY ACID OXIDATION IN ISCHEMIC/REPERFUSED AGING HEARTS
    Charles Hoppel; Fiscal Year: 1999
    ..At baseline, aging-defects in the mitochondrial electron transport chain occur in only one population of heart mitochondria (interfibrillar) in elderly Fischer 344 rats...
  26. FATTY ACID OXIDATION IN ISCHEMIC/REPERFUSED AGING HEARTS
    Charles Hoppel; Fiscal Year: 1999
    ..At baseline, aging-defects in the mitochondrial electron transport chain occur in only one population of heart mitochondria (interfibrillar) in elderly Fischer 344 rats...
  27. MITOCHONDRIAL NADH AND SUCCINATE DEHYDROGENASE
    YVES GALANTE; Fiscal Year: 1980
    ..are the following: 1) Purify the subunits of NADH dehydrogenase and succinate dehydrogenase from beef heart mitochondria and determine their complete primary structures...
  28. FATTY ACID OXIDATION IN ISCHEMIC/REPERFUSED AGING HEARTS
    Charles Hoppel; Fiscal Year: 2000
    ..At baseline, aging-defects in the mitochondrial electron transport chain occur in only one population of heart mitochondria (interfibrillar) in elderly Fischer 344 rats...
  29. CALCIUM CONTROL IN MUSKRAT HEARTS
    THOMAS MCKEAN; Fiscal Year: 1992
    ..that mitochondria isolated from muskrat hearts have a greater calcium uptake capability than guinea pig heart mitochondria and this may be of benefit in the calcium overload which may occur during hypoxia, ischemia, acidosis and ..
  30. MECHANISM OF ALLOSTERIC INFLUENCE ON ENZYME ACTIVITY
    GREGORY REINHART; Fiscal Year: 1990
    ..Coli; and NAD-dependent isocitrate dehydrogenase (ICDH) obtained from beef heart mitochondria. All of these enzymes fulfill important regulatory niches in metabolism...
  31. PYRIDINE NUCLEOTIDES AND ISCHEMIC HEART DISEASE
    CARL BERNOFSKY; Fiscal Year: 1980
    ..In the research that is proposed, isolated heart mitochondria and other subcellular components will be examined for the presence of the enzymes that are involved in the ..
  32. MECHANISM OF RESPIRATORY CONTROL IN HEART
    Alan Koretsky; Fiscal Year: 1992
    ..between phosphate levels mitochondrial redox state, and oxygen consumption will be investigated in isolated heart mitochondria. The effect of varying Ca++, and treating the heart with adrenergic agents prior to isolation of ..
  33. MITOCHONDRIAL FUNCTIONAL CHANGES IN ISCHEMIC MYOCARDIUM
    WILLIAM ROUSLIN; Fiscal Year: 1992
    ..of ATP synthesis made by the back reaction rate of the ATP synthase in intact slow versus fast heart-rate heart mitochondria; IF1 binding regulates primarily only the back reaction rate...
  34. MITOCHONDRIAL FUNCTIONAL CHANGES IN ISCHEMIC MYOCARDIUM
    WILLIAM ROUSLIN; Fiscal Year: 1991
    ..of ATP synthesis made by the back reaction rate of the ATP synthase in intact slow versus fast heart-rate heart mitochondria; IF1 binding regulates primarily only the back reaction rate...
  35. VOLATILE ANESTHETICS AND MEMBRANE-PROTEIN INTERACTION
    JAMES RICHTER; Fiscal Year: 1980
    ..will extend preliminary work by the principal who showed that halothane extracts certain proteins from beef heart mitochondria. The effect of halothane concentration on submitochondrial localization of certain enzymes and other ..
  36. CONTROL MECHANISMS IN INTERMEDIARY METABOLISM
    EARL SHRAGO; Fiscal Year: 1990
    ..Experiments will be carried out using liver and heart mitochondria, inverted submitochondrial particles and the purified ANT reconstituted into an artificial liposome system...
  37. Molecular Regulation of Cardiac KATP Channels in Ischemia
    Jonathan C Makielski; Fiscal Year: 2010
    ..a newly created panel of SUR2 antibodies and discovered novel short variants (Kd120, 68, 55, 28) in mouse heart mitochondria, with the SUR2-55 form uniquely in mitochondria and not the surface...
  38. Molecular Regulation of Cardiac KATP Channels in Ischemia
    Jonathan Makielski; Fiscal Year: 2009
    ..a newly created panel of SUR2 antibodies and discovered novel short variants (Kd120, 68, 55, 28) in mouse heart mitochondria, with the SUR2-55 form uniquely in mitochondria and not the surface...
  39. MITOCHONDRIA AND PULMONARY ENDOTHELIAL CELL DEATH
    James Peterson; Fiscal Year: 2001
    ..electron paramagnetic resonance spectroscopy and magnetic circular and linear dichroism in isolated bovine heart mitochondria and voltage sensitive dyes in intact bovine pulmonary artery endothelial cells, will provide mechanistic ..
  40. MITOCHONDRIA AND PULMONARY ENDOTHELIAL CELL DEATH
    James Peterson; Fiscal Year: 2002
    ..electron paramagnetic resonance spectroscopy and magnetic circular and linear dichroism in isolated bovine heart mitochondria and voltage sensitive dyes in intact bovine pulmonary artery endothelial cells, will provide mechanistic ..
  41. MITOCHONDRIA AND PULMONARY ENDOTHELIAL CELL DEATH
    James Peterson; Fiscal Year: 2000
    ..electron paramagnetic resonance spectroscopy and magnetic circular and linear dichroism in isolated bovine heart mitochondria and voltage sensitive dyes in intact bovine pulmonary artery endothelial cells, will provide mechanistic ..
  42. MITOCHONDRIA AND PULMONARY ENDOTHELIAL CELL DEATH
    James Peterson; Fiscal Year: 2003
    ..electron paramagnetic resonance spectroscopy and magnetic circular and linear dichroism in isolated bovine heart mitochondria and voltage sensitive dyes in intact bovine pulmonary artery endothelial cells, will provide mechanistic ..
  43. Mechanisms underlie inverse gender discrepancy in ischemic protection
    Nian Qing Shi; Fiscal Year: 2009
    ..splicing (IES) event in SUR2 mRNA to produce transcripts encoding the 55-kDa SUR2 short forms in heart mitochondria. Characterization of SUR2 KO has revealed an inverse pattern of gender difference in cardioprotection...
  44. ION TRANSPORT BY HEART MITOCHONDRIA
    GERALD BRIERLEY; Fiscal Year: 1993
    ..proposal is to isolate and characterize three monovalent cation transporters of physiological importance in heart mitochondria. These are (a) the Na+/H+ antiport and (b) the Na+/Ca2+ antiport which, acting in concert contribute to ..
  45. ION TRANSPORT BY HEART MITOCHONDRIA
    GERALD BRIERLEY; Fiscal Year: 1980
    ..of this research are to establish the mechanism by which monovalent cations are taken up and extruded by heart mitochondria and the physiological significance of the flux of K ion and Na ion across the mitochondrial membrane...
  46. ION TRANSPORT BY HEART MITOCHONDRIA
    GERALD BRIERLEY; Fiscal Year: 1990
    ..proposal is to isolate and characterize three monovalent cation transporters of physiological importance in heart mitochondria. These are (a) the Na+/H+ antiport and (b) the Na+/Ca2+ antiport which, acting in concert contribute to ..
  47. THE MECHANISM OF MITOCHONDRIAL ELECTRON TRANSFER
    PAUL LOACH; Fiscal Year: 1980
    ..rubrum (aerobically) and yeast in the presence of an appropriate precursor. Beef heart mitochondria will also be labelled by exchange in order to assess whether or not the electron transfer membranes of these ..
  48. STUDIES OF ATP SYNTHETASE FROM HEART MITOCHONDRIA
    Roderick Capaldi; Fiscal Year: 1980
    The ATP synthetase of heart mitochondria is a multicomponent complex which functions in electron transport driven ATP synthesis and as an ATPase to drive ion transport or reverse electron transport...
  49. FATTY ACID OXIDATION IN ISCHEMIC/REPERFUSED AGING HEARTS
    Charles Hoppel; Fiscal Year: 2004
    ..At baseline, aging defects in mitochondrial electron transport chain occur in only one population of heart mitochondria (interfibrillar) in elderly Fisher 344 rats...
  50. MITOCHONDRIAL HYPOXIA: PRODUCTION AND REACTION OF ROS
    Vernon Anderson; Fiscal Year: 2004
    ..Defects identified in elderly heart mitochondria are hypothesized to augment ROS production during ischemia, resulting in increased oxidative damage during ..
  51. MECHANISM(S) OF ALTERED CA2+ TRANSPORT IN ISCHEMIC HEART
    LOUIS SORDAHL; Fiscal Year: 1980
    ..will be utilized to produce depletion of internal adenine nucleotides to alter calcium kinetics in normal heart mitochondria. Changes in the mechanisms governing inorganic phosphate transport through the mitochondrial inner membrane ..
  52. Heart mitochondrial NOS and in vitro hypoxia/reperfusion
    Pedram Ghafourifar; Fiscal Year: 2004
    ..We will also test apoptosis, oxidative stress and peroxynitrite markers during hypoxia/reoxygenation in heart mitochondria of young and aged rats...