polyribosomes

Summary

Summary: A multiribosomal structure representing a linear array of RIBOSOMES held together by messenger RNA; (RNA, MESSENGER); They represent the active complexes in cellular protein synthesis and are able to incorporate amino acids into polypeptides both in vivo and in vitro. (From Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)

Top Publications

  1. pmc Polysomes, P bodies and stress granules: states and fates of eukaryotic mRNAs
    Vidya Balagopal
    Department of Molecular and Cellular Biology and Howard Hughes Medical Institute, University of Arizona, Tucson, AZ 85721 0206, USA
    Curr Opin Cell Biol 21:403-8. 2009
  2. pmc Movement of eukaryotic mRNAs between polysomes and cytoplasmic processing bodies
    Muriel Brengues
    Department of Molecular and Cellular Biology and Howard Hughes Medical Institute, University of Arizona, Tucson, AZ 85721 0106, USA
    Science 310:486-9. 2005
  3. doi RNA granules: post-transcriptional and epigenetic modulators of gene expression
    Paul Anderson
    Division of Rheumatology, Immunology and Allergy, Brigham and Women s Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA
    Nat Rev Mol Cell Biol 10:430-6. 2009
  4. pmc Identification of many microRNAs that copurify with polyribosomes in mammalian neurons
    John Kim
    Department of Molecular Biology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA
    Proc Natl Acad Sci U S A 101:360-5. 2004
  5. pmc FMRP stalls ribosomal translocation on mRNAs linked to synaptic function and autism
    Jennifer C Darnell
    Laboratory of Molecular Neuro Oncology, The Rockefeller University, New York, NY 10065, USA
    Cell 146:247-61. 2011
  6. pmc Co-translational mRNA decay in Saccharomyces cerevisiae
    Wenqian Hu
    Center for RNA Molecular Biology, Case Western Reserve University, Cleveland, Ohio 44106, USA
    Nature 461:225-9. 2009
  7. pmc Yeast translational response to high salinity: global analysis reveals regulation at multiple levels
    Daniel Melamed
    Faculty of Biology, Technion Israel Institute of Technology, Haifa 32000, Israel
    RNA 14:1337-51. 2008
  8. doi A functional RNAi screen links O-GlcNAc modification of ribosomal proteins to stress granule and processing body assembly
    Takbum Ohn
    Division of Rheumatology, Immunology and Allergy, Brigham and Women s Hospital, Harvard Medical School, 1 Jimmy Fund Way, Boston, Massachusetts 02115, USA
    Nat Cell Biol 10:1224-31. 2008
  9. ncbi Ataxin-2 and its Drosophila homolog, ATX2, physically assemble with polyribosomes
    Terrence F Satterfield
    Department of Genome Sciences, University of Washington School of Medicine, Seattle, WA 98195 7730, USA
    Hum Mol Genet 15:2523-32. 2006
  10. pmc Genome-wide analysis of mRNA translation profiles in Saccharomyces cerevisiae
    Yoav Arava
    Department of Biochemistry, Stanford University, Stanford, CA 94305 5307, USA
    Proc Natl Acad Sci U S A 100:3889-94. 2003

Detail Information

Publications244 found, 100 shown here

  1. pmc Polysomes, P bodies and stress granules: states and fates of eukaryotic mRNAs
    Vidya Balagopal
    Department of Molecular and Cellular Biology and Howard Hughes Medical Institute, University of Arizona, Tucson, AZ 85721 0206, USA
    Curr Opin Cell Biol 21:403-8. 2009
    ..Here we discuss how these different granules suggest a dynamic model for the metabolism of cytoplasmic mRNAs wherein they cycle between different mRNP states with different functional properties and subcellular locations...
  2. pmc Movement of eukaryotic mRNAs between polysomes and cytoplasmic processing bodies
    Muriel Brengues
    Department of Molecular and Cellular Biology and Howard Hughes Medical Institute, University of Arizona, Tucson, AZ 85721 0106, USA
    Science 310:486-9. 2005
    ..Moreover, the presence of related proteins in P-bodies and maternal mRNA storage granules suggests this mechanism is widely adapted for mRNA storage...
  3. doi RNA granules: post-transcriptional and epigenetic modulators of gene expression
    Paul Anderson
    Division of Rheumatology, Immunology and Allergy, Brigham and Women s Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA
    Nat Rev Mol Cell Biol 10:430-6. 2009
    ..We propose that RNA granules can be both a cause and a consequence of altered mRNA translation, decay or editing. In this capacity, RNA granules serve as key modulators of post-transcriptional and epigenetic gene expression...
  4. pmc Identification of many microRNAs that copurify with polyribosomes in mammalian neurons
    John Kim
    Department of Molecular Biology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA
    Proc Natl Acad Sci U S A 101:360-5. 2004
    ..Moreover, all of the miRNAs that were tested cofractionate with polyribosomes, the sites of active translation...
  5. pmc FMRP stalls ribosomal translocation on mRNAs linked to synaptic function and autism
    Jennifer C Darnell
    Laboratory of Molecular Neuro Oncology, The Rockefeller University, New York, NY 10065, USA
    Cell 146:247-61. 2011
    ..In addition, they provide insight into the molecular basis of the cognitive and allied defects in FXS and ASD and suggest multiple targets for clinical intervention...
  6. pmc Co-translational mRNA decay in Saccharomyces cerevisiae
    Wenqian Hu
    Center for RNA Molecular Biology, Case Western Reserve University, Cleveland, Ohio 44106, USA
    Nature 461:225-9. 2009
    ..The data indicate that dissociation of ribosomes from mRNA is not a prerequisite for decay and we suggest that the 5'-3' polarity of mRNA degradation has evolved to ensure that the last translocating ribosome can complete translation...
  7. pmc Yeast translational response to high salinity: global analysis reveals regulation at multiple levels
    Daniel Melamed
    Faculty of Biology, Technion Israel Institute of Technology, Haifa 32000, Israel
    RNA 14:1337-51. 2008
    ..Since a relatively small number of genes was affected by Hog1p deletion, additional signaling pathways are likely to be involved in coordinating the translational response to severe salinity stress...
  8. doi A functional RNAi screen links O-GlcNAc modification of ribosomal proteins to stress granule and processing body assembly
    Takbum Ohn
    Division of Rheumatology, Immunology and Allergy, Brigham and Women s Hospital, Harvard Medical School, 1 Jimmy Fund Way, Boston, Massachusetts 02115, USA
    Nat Cell Biol 10:1224-31. 2008
    ..The lack of enzymes of the hexosamine biosynthetic pathway in budding yeast may contribute to differences between mammalian SGs and related yeast EGP (eIF4E, 4G and Pab1 containing) bodies...
  9. ncbi Ataxin-2 and its Drosophila homolog, ATX2, physically assemble with polyribosomes
    Terrence F Satterfield
    Department of Genome Sciences, University of Washington School of Medicine, Seattle, WA 98195 7730, USA
    Hum Mol Genet 15:2523-32. 2006
    ..Here, we demonstrate that ataxin-2 and its Drosophila homolog, ATX2, assemble with polyribosomes and poly(A)-binding protein (PABP), a key regulator of mRNA translation...
  10. pmc Genome-wide analysis of mRNA translation profiles in Saccharomyces cerevisiae
    Yoav Arava
    Department of Biochemistry, Stanford University, Stanford, CA 94305 5307, USA
    Proc Natl Acad Sci U S A 100:3889-94. 2003
    ..Global analysis revealed an unexpected correlation: Ribosome density decreases with increasing ORF length. Models to account for this surprising observation are discussed...
  11. pmc Nonsense-mediated mRNA decapping occurs on polyribosomes in Saccharomyces cerevisiae
    Wenqian Hu
    Center for RNA Molecular Biology, Case Western Reserve University, Cleveland, Ohio, USA
    Nat Struct Mol Biol 17:244-7. 2010
    ..We show decapped nonsense-containing mRNA associate with polyribosomes, indicating that recognition and degradation are tightly coupled and that polyribosomes are major sites for ..
  12. pmc AU-rich-element-mediated upregulation of translation by FXR1 and Argonaute 2
    Shobha Vasudevan
    Department of Molecular Biophysics and Biochemistry, Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, CT 06536, USA
    Cell 128:1105-18. 2007
    ..This novel cell-growth-dependent translation activation role for FXR1 and AGO2 allows new insights into ARE-mediated signaling and connects two important posttranscriptional regulatory systems in an unexpected way...
  13. ncbi Differential mRNA translation contributes to gene regulation under non-stress and dehydration stress conditions in Arabidopsis thaliana
    Riki Kawaguchi
    Department of Botany and Plant Sciences, Center for Plant Cell Biology, University of California, Riverside, CA 92521 0124, USA
    Plant J 38:823-39. 2004
    ..Thus, the abundance of PS mRNA may provide a more accurate estimate of gene expression than total cellular mRNA because of extensive differential translational regulation...
  14. pmc eIF4GI links nutrient sensing by mTOR to cell proliferation and inhibition of autophagy
    Francisco Ramírez-Valle
    Department of Microbiology, New York University School of Medicine, New York, NY 10016, USA
    J Cell Biol 181:293-307. 2008
    ..The majority of mRNAs that were translationally impaired with eIF4GI depletion were excluded from polyribosomes due to the presence of multiple upstream open reading frames and low mRNA abundance...
  15. pmc Translational reprogramming following UVB irradiation is mediated by DNA-PKcs and allows selective recruitment to the polysomes of mRNAs encoding DNA repair enzymes
    Ian R Powley
    Centre for Biomolecular Sciences, School of Pharmacy, University of Nottingham, University Park, Nottingham NG7 2RD, United Kingdom
    Genes Dev 23:1207-20. 2009
    ....
  16. ncbi Genome-wide analysis of transcript abundance and translation in Arabidopsis seedlings subjected to oxygen deprivation
    Cristina Branco-Price
    Department of Botany and Plant Sciences, Center for Plant Cell Biology, University of California, Riverside, CA 92521, USA
    Ann Bot 96:647-60. 2005
    ..The aim of this study was to evaluate the role of translational regulation in response to oxygen deprivation (hypoxia)...
  17. ncbi Mammalian Smaug is a translational repressor that forms cytoplasmic foci similar to stress granules
    María V Baez
    Fundacion Instituto Leloir, IIBBA CONICET and IIB Facultad de Ciencias Exactas y Naturales, University of Buenos Aires, C1405BWE Buenos Aires, Argentina
    J Biol Chem 280:43131-40. 2005
    ..Biochemical analysis indicated that mSmaug 1 is present in synaptoneurosomal 20 S particles. These results suggest a role for mammalian Smaug 1 in RNA granule formation and translation regulation in neurons...
  18. doi Isolation of plant polysomal mRNA by differential centrifugation and ribosome immunopurification methods
    Angelika Mustroph
    Center for Plant Cell Biology and Department of Botany and Plant Sciences, University of California, Riverside, CA, USA
    Methods Mol Biol 553:109-26. 2009
    b>Polyribosomes (polysomes) form as multiple ribosomes engage in translation on a single mRNA. This process is regulated for individual mRNAs by both development and the environment...
  19. pmc Decapping and decay of messenger RNA occur in cytoplasmic processing bodies
    Ujwal Sheth
    Department of Molecular and Cellular Biology and Howard Hughes Medical Institute, University of Arizona, Tucson, AZ 85721, USA
    Science 300:805-8. 2003
    ..These results define the flux of mRNAs between polysomes and P bodies as a critical aspect of cytoplasmic mRNA metabolism and a possible site for regulation of mRNA degradation...
  20. doi Posttranscriptional mechanisms involved in the control of expression of the stage-specific GP82 surface glycoprotein in Trypanosoma cruzi
    Luciana Girotto Gentil
    Department of Microbiology, Immunology and Parasitology, Escola Paulista de Medicina, UNIFESP, Rua Botucatu, Sao Paulo, Brazil
    Acta Trop 109:152-8. 2009
    ..Consistent with this finding, northern blot analysis showed that GP82 mRNAs were mobilized to polysomes and consequently translated, but only in metacyclic trypomastigotes...
  21. pmc Biochemical evidence for the association of fragile X mental retardation protein with brain polyribosomal ribonucleoparticles
    Edouard W Khandjian
    Unite de Recherche en Genetique Humaine et Moleculaire, Centre de recherche Hôpital Saint François d Assise, Centre Hospitalier Universitaire de Quebec, Quebec, QC, Canada G1L 3L5
    Proc Natl Acad Sci U S A 101:13357-62. 2004
    ..We demonstrate that, in the brain, FMRP is present in actively translating polyribosomes, and we show that this association is acutely sensitive to the type of detergent required to release ..
  22. doi Translational regulation of the human achaete-scute homologue-1 by fragile X mental retardation protein
    Michael Fähling
    Charite, Universitatsmedizin Berlin, Institut für Vegetative Physiologie, Tucholskystrasse 2, D 10117 Berlin
    J Biol Chem 284:4255-66. 2009
    ..In conclusion, we identified hASH1 as a novel downstream target of FMRP. Improved translation efficiency of hASH1 mRNA by FMRP may represent an important regulatory switch in neuronal differentiation...
  23. doi PKCbetaII modulates translation independently from mTOR and through RACK1
    Stefano Grosso
    Molecular Histology and Cell Growth, HSR, 20132 Milan, Italy
    Biochem J 415:77-85. 2008
    ..Taken together the results of the present study show that PKCbetaII can act as a specific PKC isoform regulating translation, in an mTOR-independent fashion, possibly close to the ribosomal machinery...
  24. pmc Activated platelets mediate inflammatory signaling by regulated interleukin 1beta synthesis
    S Lindemann
    Department of Internal Medicine, University of Utah, Salt Lake City, UT 84112, USA
    J Cell Biol 154:485-90. 2001
    ....
  25. ncbi TOPs and their regulation
    T L Hamilton
    School of Pharmacy, University of Nottingham, University Park, Nottingham, NG7 2RD, UK
    Biochem Soc Trans 34:12-6. 2006
    ....
  26. pmc Translation of a small subset of Caenorhabditis elegans mRNAs is dependent on a specific eukaryotic translation initiation factor 4E isoform
    Tzvetanka D Dinkova
    Department of Biochemistry and Molecular Biology, Louisiana State University Health Sciences Center, Shreveport, LA 71130 3932, USA
    Mol Cell Biol 25:100-13. 2005
    ..The functions of these proteins can explain some phenotypes observed in ife-4 knockout mutants. These results indicate that translation of a limited subset of mRNAs is dependent on a specific isoform of eIF4E...
  27. pmc Stress granules and processing bodies are dynamically linked sites of mRNP remodeling
    Nancy Kedersha
    Division of Rheumatology and Immunology, Harvard Medical School, Brigham and Women s Hospital, Boston, MA 02115, USA
    J Cell Biol 169:871-84. 2005
    ..We propose that mRNA released from disassembled polysomes is sorted and remodeled at SGs, from which selected transcripts are delivered to PBs for degradation...
  28. ncbi In vivo stabilization of preinitiation complexes by formaldehyde cross-linking
    LEOS VALASEK
    Institute of Microbiology, AS CR, Prague, Czech Republic
    Methods Enzymol 429:163-83. 2007
    ..In addition, different conditions for displaying the polysome profile or PIC analysis by SDC, used to address different questions, will be outlined...
  29. pmc RAS/ERK signaling promotes site-specific ribosomal protein S6 phosphorylation via RSK and stimulates cap-dependent translation
    Philippe P Roux
    Department of Pathology and Cell Biology, Institut de recherche en immunologie et en cancérologie IRIC, Universite de Montreal, Montreal, Quebec, Canada
    J Biol Chem 282:14056-64. 2007
    ..These data demonstrate that RSK provides an mTOR-independent pathway linking the Ras/ERK signaling cascade to the translational machinery...
  30. pmc Nip7p interacts with Nop8p, an essential nucleolar protein required for 60S ribosome biogenesis, and the exosome subunit Rrp43p
    N I Zanchin
    Department of Biology, University of Rochester, Rochester, New York 14627, USA
    Mol Cell Biol 19:1518-25. 1999
    ..Distinct pools of Rrp43p may interact both with the exosome and with Nip7p, possibly both in the nucleus and in the cytoplasm, to catalyze analogous reactions in the multistep process of 60S ribosome biogenesis and mRNA turnover...
  31. ncbi Evidence that microRNAs are associated with translating messenger RNAs in human cells
    Patricia A Maroney
    Center for RNA Molecular Biology and Department of Biochemistry, Case Western Reserve University School of Medicine, W127 10900 Euclid Avenue, Cleveland, Ohio 44106 4973, USA
    Nat Struct Mol Biol 13:1102-7. 2006
    ..Several lines of evidence indicate that most of these mRNAs, including a known miRNA-regulated target (KRAS mRNA), are actively being translated...
  32. pmc P-body formation is a consequence, not the cause, of RNA-mediated gene silencing
    Ana Eulalio
    Max Planck Institute for Developmental Biology, Spemannstrasse 35, D 72076 Tubingen, Germany
    Mol Cell Biol 27:3970-81. 2007
    ..Thus, even though P-body components play crucial roles in mRNA silencing and decay, aggregation into P bodies is not required for function but is instead a consequence of their activity...
  33. ncbi The lin-4 regulatory RNA controls developmental timing in Caenorhabditis elegans by blocking LIN-14 protein synthesis after the initiation of translation
    P H Olsen
    Department of Biological Sciences, Dartmouth College, Hanover, New Hampshire 03755, USA
    Dev Biol 216:671-80. 1999
    ....
  34. ncbi The translational efficiencies of the two Leishmania infantum HSP70 mRNAs, differing in their 3'-untranslated regions, are affected by shifts in the temperature of growth through different mechanisms
    Cristina Folgueira
    Centro de Biologia Molecular Severo Ochoa, Universidad Autonoma de Madrid, 28049 Madrid, Spain
    J Biol Chem 280:35172-83. 2005
    ..Remarkably, the deltaHSP70 mutant synthesizes HSP70 at a lower rate than the wild-type parasites. Overall, our data suggest that the biological function of the HSP70-II gene is to top up HSP70 levels under conditions of stress...
  35. pmc Fear and safety learning differentially affect synapse size and dendritic translation in the lateral amygdala
    Linnaea E Ostroff
    Center for Neural Science, New York University, New York, NY 10003, USA
    Proc Natl Acad Sci U S A 107:9418-23. 2010
    ..There were more polyribosomes and multivesicular bodies throughout the dendrites from fear conditioned rats, indicating increases in both ..
  36. ncbi Polyribosomes are increased in spines of CA1 dendrites 2 h after the induction of LTP in mature rat hippocampal slices
    Jennifer N Bourne
    Synapses and Cognitive Neuroscience Center, Medical College of Georgia, Augusta, Georgia, USA
    Hippocampus 17:1-4. 2007
    Enduring long-term potentiation (LTP) requires immediate protein synthesis, hence we assessed whether more polyribosomes are present in dendritic spines of mature hippocampal dendrites after the induction of LTP...
  37. pmc A network of multiple regulatory layers shapes gene expression in fission yeast
    Daniel H Lackner
    Cancer Research UK Fission Yeast Functional Genomics Group, Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK
    Mol Cell 26:145-55. 2007
    ..These rich data sets, all acquired under a standardized condition, reveal a substantial coordination between regulatory layers and provide a basis for a systems-level understanding of multilayered gene-expression programs...
  38. pmc IMP dehydrogenase type 1 associates with polyribosomes translating rhodopsin mRNA
    Sarah E Mortimer
    Department of Biochemistry, Brandeis University, Waltham, Massachusetts 02454 9110, USA
    J Biol Chem 283:36354-60. 2008
    ..Here we show that both IMPDH type 1 (IMPDH1) and IMPDH type 2 are associated with polyribosomes, suggesting that these housekeeping proteins have an unanticipated role in translation regulation...
  39. pmc Translation of aberrant mRNAs lacking a termination codon or with a shortened 3'-UTR is repressed after initiation in yeast
    Toshifumi Inada
    Department of Molecular Biology, Graduate School of Science, Nagoya University, Nagoya, Japan
    EMBO J 24:1584-95. 2005
    ..We also found that translation of aberrant mRNAs with a shortened 3'-UTR was repressed, suggesting that an improper spatial distance between the termination codon and the 3' end of mRNA results in translation repression...
  40. doi Phosphorylation of eIF4E by MNKs supports protein synthesis, cell cycle progression and proliferation in prostate cancer cells
    Andrea Bianchini
    Department of Public Health and Cell Biology, University of Rome Tor Vergata, Rome, Italy
    Carcinogenesis 29:2279-88. 2008
    ....
  41. doi Saccharomyces cerevisiae Gis2 interacts with the translation machinery and is orthogonal to myotonic dystrophy type 2 protein ZNF9
    Morgan A Sammons
    Department of Biological Sciences, Vanderbilt University, Nashville, TN 37232, USA
    Biochem Biophys Res Commun 406:13-9. 2011
    ..These data suggest that Gis2 is functionally orthologous to ZNF9 and acts as a cap-independent translation factor...
  42. pmc Isolation of polysome-bound mRNA from solid tissues amenable for RT-PCR and profiling experiments
    M Julieta Del Prete
    Department of Immunology, Centro de Investigaciones Biologicas, Madrid, Spain
    RNA 13:414-21. 2007
    ....
  43. pmc Chloroplast biogenesis of photosystem II cores involves a series of assembly-controlled steps that regulate translation
    Limor Minai
    Unité Mixte de Recherche 7141, Centre National de la Recherche Scientifique Université Pierre et Marie Curie Paris VI, Institut de Biologie Physico Chimique, France
    Plant Cell 18:159-75. 2006
    ..De novo synthesis and repair of photosystem II complexes are independently controlled...
  44. pmc Immunopurification of polyribosomal complexes of Arabidopsis for global analysis of gene expression
    Maria Eugenia Zanetti
    Department of Botany and Plant Science, Center for Plant Cell Biology, University of California, Riverside, California 92521 0124, USA
    Plant Physiol 138:624-35. 2005
    Immunoaffinity purification of polyribosomes (polysomes) from crude leaf extracts of Arabidopsis (Arabidopsis thaliana) was achieved with transgenic genotypes that overexpress a translational fusion of a ribosomal protein (RP) with a His(..
  45. pmc Inhibition of polysome assembly enhances imatinib activity against chronic myelogenous leukemia and overcomes imatinib resistance
    Min Zhang
    Division of Hematology Oncology, Department of Medicine, University of California at Irvine, Irvine, California 92697, USA
    Mol Cell Biol 28:6496-509. 2008
    ..Together, this work supports the inhibition of translation initiation as a therapeutic strategy for treating cancers fueled by dysregulated translation...
  46. pmc Novel zinc-responsive post-transcriptional mechanisms reciprocally regulate expression of the mouse Slc39a4 and Slc39a5 zinc transporters (Zip4 and Zip5)
    Benjamin P Weaver
    Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City, KS 66160 7421, USA
    Biol Chem 388:1301-12. 2007
    ..After zinc-gavage, ZIP5 is rapidly resynthesized and targeted to the basolateral membranes of these cell types...
  47. pmc Aldosterone and vasopressin affect {alpha}- and {gamma}-ENaC mRNA translation
    Andrea Perlewitz
    Institut für Vegetative Physiologie, Universitatsmedizin Berlin Charite, D 10117 Berlin, Germany
    Nucleic Acids Res 38:5746-60. 2010
    ..Immunoprecipitation and UV-crosslinking analysis of γ-ENaC-mRNA/HuR complexes document the significance of γ-ENaC-mRNA-3'-UTR/HuR interaction for hormonal control of ENaC synthesis...
  48. ncbi Developmental expression of the fragile X-related 1 proteins in mouse testis: association with microtubule elements
    M E Huot
    , , 10, rue de l'Espinay, , Canada
    Hum Mol Genet 10:2803-11. 2001
    ..Since the cytoskeletal framework is implicated in cellular plasticity as well as in mRNA transport, we propose new possibilities for the function(s) of the FXR proteins...
  49. pmc The RACK1 signal anchor protein from Trypanosoma brucei associates with eukaryotic elongation factor 1A: a role for translational control in cytokinesis
    Sandesh Regmi
    Department of Biological Sciences, Southern Methodist University, Dallas, TX 75275, USA
    Mol Microbiol 70:724-45. 2008
    ..The synergy between anisomycin and TbRACK1 RNAi suggests that continued translation is required for complete ingression of the cleavage furrow...
  50. pmc Functional overlap between eIF4G isoforms in Saccharomyces cerevisiae
    Bryan K Clarkson
    Department of Molecular and Cell Biology, University of California, Berkeley, California, United States of America
    PLoS ONE 5:e9114. 2010
    ..These results suggest that total eIF4G levels, but not isoform-specific functions, determine mRNA-specific translational efficiency...
  51. pmc Stress-activated protein kinase pathway functions to support protein synthesis and translational adaptation in response to environmental stress in fission yeast
    Isabelle Dunand-Sauthier
    MRC Radiation and Genome Stability Unit, Harwell, Didcot, United Kingdom, and Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis, USA
    Eukaryot Cell 4:1785-93. 2005
    ..These findings suggest a role for the SAPK pathway in supporting translation initiation and facilitating adaptation to environmental stress in part through reducing eIF2alpha phosphorylation in fission yeast...
  52. pmc Amino acid-induced translation of TOP mRNAs is fully dependent on phosphatidylinositol 3-kinase-mediated signaling, is partially inhibited by rapamycin, and is independent of S6K1 and rpS6 phosphorylation
    H Tang
    Department of Biochemistry, The Hebrew University-Hadassah Medical School, Jerusalem 91120, Israel
    Mol Cell Biol 21:8671-83. 2001
    ....
  53. ncbi FMRP associates with polyribosomes as an mRNP, and the I304N mutation of severe fragile X syndrome abolishes this association
    Y Feng
    Howard Hughes Medical Institute, Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    Mol Cell 1:109-18. 1997
    ..We show here that normal FMRP associates with elongating polyribosomes via large mRNP particles...
  54. doi Plant stress granules and mRNA processing bodies are distinct from heat stress granules
    Christian Weber
    Department of Molecular Cell Biology, Johann Wolfgang Goethe University Frankfurt, Max von Laue Strasse 9, 60438 Frankfurt, Germany
    Plant J 56:517-30. 2008
    ..SGs and PBs can be clearly discriminated from the previously described heat stress granules (HSGs), which evidently do not contain mRNPs. Thus, the role of HSGs as putative mRNP storage sites must be revised...
  55. ncbi mRNPs, polysomes or granules: FMRP in neuronal protein synthesis
    Francesca Zalfa
    Dipartimento di Biologia, Universita di Roma Tor Vergata, Via della Ricerca Scientifica 1, 00133, Roma, Italy
    Curr Opin Neurobiol 16:265-9. 2006
    ....
  56. ncbi Fragile X mental retardation protein shifts between polyribosomes and stress granules after neuronal injury by arsenite stress or in vivo hippocampal electrode insertion
    Soong Ho Kim
    Beckman Institute, University of Illinois at Urbana Champaign, Urbana, Illinois 61801, USA
    J Neurosci 26:2413-8. 2006
    ..by virtue of their TIA-1 (T-cell intracellular antigen 1) protein component, and found that FMRP moved out of polyribosomes and into SGs subsequent to oxidative stress...
  57. ncbi The Shwachman-Bodian-Diamond syndrome associated protein interacts with HsNip7 and its down-regulation affects gene expression at the transcriptional and translational levels
    Cédric Hesling
    Center for Structural Molecular Biology, Brazilian Synchrotron Light Laboratory, LNLS Rua Giuseppe Maximo Scolfaro 10000, PO Box 6192, CEP 13084 971, Campinas, SP, Brazil
    Exp Cell Res 313:4180-95. 2007
    ..These results indicate that SBDS is required for accurate expression of genes important for proper brain, skeletal, and blood cell development...
  58. ncbi Analysis of polysomal mRNA populations of mouse oocytes and zygotes: dynamic changes in maternal mRNA utilization and function
    Santhi Potireddy
    The Department of Biochemistry, Temple University School of Medicine, Philadelphia, PA 19140, USA
    Dev Biol 298:155-66. 2006
    ..We observe global transitions in the functional classes of translated MmRNAs and apparent changes in the underlying cis-regulatory mechanisms...
  59. ncbi Trapping of messenger RNA by Fragile X Mental Retardation protein into cytoplasmic granules induces translation repression
    Rachid Mazroui
    Unite de Recherche en Genetique Humaine et Moleculaire, Centre de recherche Hôpital Saint François d Assise, 10 rue de l Espinay, Quebec G1L 3L5, PQ, Canada
    Hum Mol Genet 11:3007-17. 2002
    ..The FMRP-containing mRNPs are dynamic structures that oscillate between polyribosomes and cytoplasmic granules reminiscent of the Stress Granules that contain repressed mRNAs...
  60. pmc A novel function for fragile X mental retardation protein in translational activation
    Elias G Bechara
    Institut de Pharmacologie Moleculaire et Cellulaire, Valbonne, France
    PLoS Biol 7:e16. 2009
    ....
  61. pmc Staufen recruitment into stress granules does not affect early mRNA transport in oligodendrocytes
    María G Thomas
    Fundacion Instituto Leloir, IIB Facultad de Ciencias Exactas y Naturales, University of Buenos Aires, IIBBA CONICET, Buenos Aires, Argentina
    Mol Biol Cell 16:405-20. 2005
    ....
  62. doi Selective mRNA translation coordinates energetic and metabolic adjustments to cellular oxygen deprivation and reoxygenation in Arabidopsis thaliana
    Cristina Branco-Price
    Center for Plant Cell Biology, University of California, Riverside, CA 92521, USA
    Plant J 56:743-55. 2008
    ..Hypoxia stress and reoxygenation promoted adjustments in the levels of polyribosomes (polysomes) that were highly coordinated with cellular ATP content...
  63. ncbi Gonadotropin-regulated testicular RNA helicase (GRTH/Ddx25) is a transport protein involved in gene-specific mRNA export and protein translation during spermatogenesis
    Yi Sheng
    Section on Molecular Endocrinology, Endocrinology and Reproduction Research Branch, NICHD, National Institutes of Health, Bethesda, Maryland 20892 4510, USA
    J Biol Chem 281:35048-56. 2006
    ..The phosphorylated cytoplasmic 61-kDa species was associated with polyribosomes. Confocal studies on COS-1 cells showed that GRTH-GFP was retained in the nucleus by treatment with a RNA ..
  64. ncbi Stressful initiations
    Paul Anderson
    Division of Rheumatology, Immunology and Allergy, Brigham and Women s Hospital, Smith 652, One Jimmy Fund Way, Boston, MA 02115, USA
    J Cell Sci 115:3227-34. 2002
    ....
  65. ncbi Global and specific translational control by rapamycin in T cells uncovered by microarrays and proteomics
    Annabelle Grolleau
    Department of Microbiology and Immunology, University of Michigan, Ann Arbor, Michigan 48109, USA
    J Biol Chem 277:22175-84. 2002
    ....
  66. ncbi Phosphorylation influences the translation state of FMRP-associated polyribosomes
    Stephanie Ceman
    Department of Human Genetics, Emory University School of Medicine, Atlanta, GA 30322, USA
    Hum Mol Genet 12:3295-305. 2003
    ..FMRP is a nucleocytoplasmic RNA binding protein that is primarily associated with polyribosomes. FMRP is believed to be a translational repressor and may regulate the translation of certain mRNAs at the ..
  67. doi Akt phosphorylation of La regulates specific mRNA translation in glial progenitors
    F Brenet
    Department of Cancer Biology and Genetics, Memorial Sloan Kettering Cancer Center, New York, NY, USA
    Oncogene 28:128-39. 2009
    ..Therefore, La appears to be an important contributor to Akt-mediated translational regulation of these transcripts in murine glial cells...
  68. pmc Expression profiling reveals meiotic male germ cell mRNAs that are translationally up- and down-regulated
    Naoko Iguchi
    Center for Research on Reproduction and Women s Health, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Proc Natl Acad Sci U S A 103:7712-7. 2006
    ..This high-throughput approach defines the changing translation patterns of populations of genes as male germ cells differentiate and identifies groups of meiotic transcripts that are translationally up- and down-regulated...
  69. ncbi Heme-regulated inhibitor kinase-mediated phosphorylation of eukaryotic translation initiation factor 2 inhibits translation, induces stress granule formation, and mediates survival upon arsenite exposure
    Edward McEwen
    Howard Hughes Medical Institute and the Department of Biological Chemistry, University of Michigan Medical Center, Ann Arbor 48109, USA
    J Biol Chem 280:16925-33. 2005
    ..The results demonstrated an essential role for HRI in mediating arsenite stress-induced phosphorylation of eukaryotic translation initiation factor 2alpha, inhibition of protein synthesis, stress granule formation, and survival...
  70. doi Acidic stress induces the formation of P-bodies, but not stress granules, with mild attenuation of bulk translation in Saccharomyces cerevisiae
    Aya Iwaki
    Laboratory of Microbial Technology, Graduate School of Science and Technology, Kyoto Institute of Technology, Matsugasaki, Kyoto 606 8585, Japan
    Biochem J 446:225-33. 2012
    ....
  71. pmc Availability of Rubisco small subunit up-regulates the transcript levels of large subunit for stoichiometric assembly of its holoenzyme in rice
    Yuji Suzuki
    Graduate School of Agricultural Science, Tohoku University, Sendai 981 8555, Japan
    Plant Physiol 160:533-40. 2012
    ..These results demonstrate that the availability of RBCS protein up-regulates the gene expression of rbcL primarily at the transcript level in a quantitative manner for stoichiometric assembly of Rubisco holoenzyme...
  72. ncbi Global translational responses to oxidative stress impact upon multiple levels of protein synthesis
    Daniel Shenton
    Faculty of Life Sciences, University of Manchester, Manchester M13 9PT, United Kingdom
    J Biol Chem 281:29011-21. 2006
    ..In summary, oxidative stress elicits complex translational reprogramming that is fundamental for adaptation to the stress...
  73. doi Stochastic theory of protein synthesis and polysome: ribosome profile on a single mRNA transcript
    Ajeet K Sharma
    Department of Physics, Indian Institute of Technology, Kanpur 208016, India
    J Theor Biol 289:36-46. 2011
    ..The theoretical framework developed here will serve in guiding further experiments and in analyzing the data to gain deep insight into various kinetic processes involved in translation...
  74. ncbi Synthesis of the posterior determinant Nanos is spatially restricted by a novel cotranslational regulatory mechanism
    I E Clark
    Department of Molecular Biology, Princeton University, New Jersey 08544, USA
    Curr Biol 10:1311-4. 2000
    ..Thus, synthesis of ectopic Nos protein is inhibited by a novel regulatory mechanism that does not involve a stable arrest of the translation cycle...
  75. ncbi Relationship between yeast polyribosomes and Upf proteins required for nonsense mRNA decay
    A L Atkin
    School of Biological Sciences, University of Nebraska, Lincoln, Nebraska 68588 0666, USA
    J Biol Chem 272:22163-72. 1997
    ..of soluble lysates and found that all three proteins co-distributed with 80 S ribosomal particles and polyribosomes. Treatment of lysates with RNase A caused a coincident collapse of polyribosomes and each Upf protein into ..
  76. ncbi SSL1, a suppressor of a HIS4 5'-UTR stem-loop mutation, is essential for translation initiation and affects UV resistance in yeast
    H Yoon
    Department of Biology, Indiana University, Bloomington 47405
    Genes Dev 6:2463-77. 1992
    ..In light of the related effects of mutations in the SSL1 and SSL2 genes, the encoded proteins may functionally interact both to promote DNA repair and perform an essential function during translation initiation...
  77. doi The three-dimensional organization of polyribosomes in intact human cells
    Florian Brandt
    Department of Molecular Structural Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
    Mol Cell 39:560-9. 2010
    ..The distinct neighbor orientations found in situ resemble configurations of bacterial polysomes in vitro, indicating a conserved supramolecular organization with implications for nascent polypeptide folding...
  78. pmc The mechanism of micro-RNA-mediated translation repression is determined by the promoter of the target gene
    Yi Wen Kong
    School of Pharmacy, Centre for Biomolecular Sciences, University of Nottingham, University Park, Nottingham NG7 2RD, United Kingdom
    Proc Natl Acad Sci U S A 105:8866-71. 2008
    ..Overall, these data establish a link between the nuclear history of an mRNA and the mechanism of miRNA-mediated translational regulation in the cytoplasm...
  79. pmc Why Dom34 stimulates growth of cells with defects of 40S ribosomal subunit biosynthesis
    Arpita Bhattacharya
    Department of Cell Biology, Albert Einstein College of Medicine, 1300 Morris Avenue, Bronx, NY 10461, USA
    Mol Cell Biol 30:5562-71. 2010
    ....
  80. pmc Identification and stage-specific association with the translational apparatus of TbZFP3, a CCCH protein that promotes trypanosome life-cycle development
    Athina Paterou
    Institute of Immunology and Infection Research, School of Biological Sciences, Ashworth Laboratories, King s Buildings, University of Edinburgh, West Mains Road, Edinburgh EH9 3JT, Scotland, United Kingdom
    J Biol Chem 281:39002-13. 2006
    ..although TbZFP3 is constitutively expressed, it exhibits developmentally regulated association with polyribosomes, and mutational analysis demonstrates that this association is essential for the expression of phenotype...
  81. pmc A model for protein translation: polysome self-organization leads to maximum protein synthesis rates
    Hermioni Zouridis
    Department of Chemical and Biological Engineering, McCormick School of Engineering and Applied Sciences, Northwestern University, Evanston, Illinois, USA
    Biophys J 92:717-30. 2007
    ....
  82. pmc Polyribosome analysis for investigating mRNA translation in Xenopus oocytes, eggs and embryos
    M D Sheets
    University of Wisconsin School of Medicine and Public Health, Department of Biomolecular Chemistry, Madison, WI 53706, USA
    Methods 51:152-6. 2010
    ..assay of an mRNA's translational state is polyribosome association; mRNAs actively translated are engaged with polyribosomes while mRNAs translationally repressed are not...
  83. ncbi Lost once, the Fragile X Mental Retardation protein is now back onto brain polyribosomes
    Laetitia Davidovic
    Unite de Recherche en Genetique Humaine et Moleculaire, Centre de recherche Hôpital Saint François d Assise, le CHUQ, Quebec, Canada
    RNA Biol 2:1-3. 2005
    ..is not associated with the translation machinery, but is part of repressed small RNP complexes excluded from polyribosomes.(27) To elucidate this puzzling result, Stefani et al.(17) and Khandjian et al...
  84. pmc Arginines of the RGG box regulate FMRP association with polyribosomes and mRNA
    Ernest Blackwell
    Department of Cell and Developmental Biology, College of Medicine, University of Illinois, Urbana Champaign, IL 61801, USA
    Hum Mol Genet 19:1314-23. 2010
    ..Finally, we show that PRMT1 methylates FMRP in cells, suggesting a model where methylation of the RGG box modulates either the quantity or the identity of the RNAs bound by FMRP...
  85. pmc Stm1p alters the ribosome association of eukaryotic elongation factor 3 and affects translation elongation
    Natalya Van Dyke
    Department of Molecular and Cellular Oncology, Unit 079, The University of Texas M D Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, TX 77030 4009, USA
    Nucleic Acids Res 37:6116-25. 2009
    ..In addition, ribosomes with increased levels of Stm1p exhibit decreased association with eEF3. Taken together, our data indicate that Stm1p plays a complementary role to eEF3 in translation...
  86. pmc The virion-packaged endoribonuclease of herpes simplex virus 1 cleaves mRNA in polyribosomes
    Brunella Taddeo
    The Marjorie B Kovler Viral Oncology Laboratories, University of Chicago, Chicago, IL 60637, USA
    Proc Natl Acad Sci U S A 106:12139-44. 2009
    ..Additional studies revealed that polyribosomes extracted from cytoplasm of wild-type virus-infected cells treated with CHX and displayed in sucrose gradients ..
  87. ncbi Hypoxia and regulation of messenger RNA translation
    Marianne Koritzinsky
    Department of Radiation Oncology Maastro Lab, GROW Research Institute, Maastricht University, Maastricht, The Netherlands
    Methods Enzymol 435:247-73. 2007
    ..We have emphasized the qualitative and quantitative analysis of polysome profiles, which can yield interesting information regarding the mechanistic basis for (gene-specific) translational regulation...
  88. ncbi Cell cycle-regulated modification of the ribosome by a variant multiubiquitin chain
    J Spence
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Cell 102:67-76. 2000
    ..L28, like other ribosomal proteins, is metabolically stable. Therefore, these data suggest a regulatory role for multiubiquitin chains that is reversible and does not function to target the acceptor protein for degradation...
  89. ncbi Fragile X mental retardation protein induces synapse loss through acute postsynaptic translational regulation
    Brad E Pfeiffer
    Center for Basic Neuroscience, Department of Physiology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    J Neurosci 27:3120-30. 2007
    ..K homology domain 2 (KH2) RNA-binding domain and dephosphorylation of FMRP at S500 were required for the effects of FMRP on synapse number, indicating that FMRP interaction with RNA and translating polyribosomes leads to synapse loss.
  90. pmc mRNA sequence features that contribute to translational regulation in Arabidopsis
    Riki Kawaguchi
    Department of Botany and Plant Sciences, Center for Plant Cell Biology, University of California Riverside, CA 92521, USA
    Nucleic Acids Res 33:955-65. 2005
    ..Notably, the mRNA features that contribute to translational regulation could not fully explain the variation in ribosome loading, indicating that additional factors contribute to translational regulation in Arabidopsis...
  91. pmc Step-wise formation of eukaryotic double-row polyribosomes and circular translation of polysomal mRNA
    Gelina S Kopeina
    Institute of Protein Research, Russian Academy of Sciences, 142290 Pushchino, Moscow Region, Russia
    Nucleic Acids Res 36:2476-88. 2008
    ..Removal or replacements of 5' and 3' UTRs affected the initial phase of translation, but did not prevent the formation of the double-row polysomes during translation...
  92. ncbi Genome-wide analysis of mRNA polysomal profiles with spotted DNA microarrays
    Daniel Melamed
    Department of Biology, Technion Israel Institute of Technology, Haifa, Israel
    Methods Enzymol 431:177-201. 2007
    ..We then provide a detailed protocol for analysis of polysomal fractions using spotted DNA microarrays...
  93. pmc The duplicated Saccharomyces cerevisiae gene SSM1 encodes a eucaryotic homolog of the eubacterial and archaebacterial L1 ribosomal proteins
    A Petitjean
    Centre de Genetique Moleculaire, Universite Pierre et Marie Curie, Gif sur Yvette, France
    Mol Cell Biol 15:5071-81. 1995
    ..Polysome profile analysis suggests that the primary defect caused by the depletion in Ssm1p is at the level of translation initiation...
  94. pmc The 39-kilodalton subunit of eukaryotic translation initiation factor 3 is essential for the complex's integrity and for cell viability in Saccharomyces cerevisiae
    T Naranda
    Department of Biological Chemistry, School of Medicine, University of California, Davis 95616, USA
    Mol Cell Biol 17:145-53. 1997
    ....
  95. pmc Adenovirus virus-associated RNAII-derived small RNAs are efficiently incorporated into the rna-induced silencing complex and associate with polyribosomes
    Ning Xu
    Department of Medical Biochemistry and Microbiology, Uppsala Biomedical Center, Husargatan 3, S 751 23 Uppsala, Sweden
    J Virol 81:10540-9. 2007
    ..Potentially, they function as miRNAs, regulating translation of cellular mRNAs. In support of this hypothesis, we detected a fraction of the VA RNAII-derived mivaRNAs on polyribosomes.
  96. pmc ACCUMULATION OF PHOTOSYSTEM ONE1, a member of a novel gene family, is required for accumulation of [4Fe-4S] cluster-containing chloroplast complexes and antenna proteins
    Katrin Amann
    Ludwig Maximilians Universitat Munchen, Department Biologie I, Botanik, 80638 Munich, Germany
    Plant Cell 16:3084-97. 2004
    ..Taken together, our findings suggest that APO1 is involved in the stable assembly of several [4Fe-4S] cluster-containing complexes of chloroplasts and interferes with translational events probably in association with plastid nucleoids...
  97. ncbi A plant viral "reinitiation" factor interacts with the host translational machinery
    H S Park
    Friedrich Miescher-Institute, P.O. Box 2543, CH-4002, Basel, Switzerland
    Cell 106:723-33. 2001
    ....
  98. pmc Ribosomal slowdown mediates translational arrest during cellular division
    Gilad Sivan
    Department of Cell Research and Immunology, George S Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv, Israel
    Mol Cell Biol 27:6639-46. 2007
    ..Stalling translating ribosomes during mitosis may protect mRNAs and allow rapid resumption of translation immediately upon entry into the G(1) phase...
  99. ncbi The shuttling SR protein 9G8 plays a role in translation of unspliced mRNA containing a constitutive transport element
    Jennifer E Swartz
    Myles H Thaler Center for AIDS and Human Retrovirus Research and Department of Microbiology, University of Virginia, Charlottesville, Virginia 22908, USA
    J Biol Chem 282:19844-53. 2007
    ..Hyperphosphorylated 9G8 was present in monosomes and small polyribosomes, whereas soluble fractions contained only hypophosphorylated protein...
  100. pmc Orphan receptor DAX-1 is a shuttling RNA binding protein associated with polyribosomes via mRNA
    E Lalli
    Institut de Genetique et de Biologie Moleculaire et Cellulaire, CNRS, INSERM, Universite Louis Pasteur, Illkirch Strasbourg, France
    Mol Cell Biol 20:4910-21. 2000
    ..A significant proportion of DAX-1 is associated with polyribosomes and is found complexed with polyadenylated RNA...
  101. ncbi Association of the mouse infertility factor DAZL1 with actively translating polyribosomes
    S Tsui
    Department of Pediatrics, Harbor UCLA Medical Center, Torrance, California 90509, USA
    Biol Reprod 62:1655-60. 2000
    ..The sedimentation profiles of DAZL1 in sucrose gradients indicate that DAZL1 is associated with polyribosomes, and further capture of DAZL1 on oligo(dT) beads demonstrates that the association is mediated through the ..

Research Grants80

  1. RiboTag: A novel technique to profile cell type specific gene expression and inv
    GEORGE STANLEY contact MCKNIGHT; Fiscal Year: 2010
    ..The polyribosomes containing the transcribed and translatable mRNAs are isolated by immunological techniques and then the RNA is ..
  2. Distance-Dependent Structure and Function of Neuronal Dendrites
    Kristen Harris; Fiscal Year: 2009
    ..along dendrites and into dendritic spines that host excitory synapses, including microtubules for transport, polyribosomes for protein synthesis, Golgi apparatus for posttranslational modifications, endosomes for membrane recycling, ..
  3. Distance-Dependent Structure and Function of Neuronal Dendrites
    Kristen M Harris; Fiscal Year: 2010
    ..along dendrites and into dendritic spines that host excitory synapses, including microtubules for transport, polyribosomes for protein synthesis, Golgi apparatus for posttranslational modifications, endosomes for membrane recycling, ..
  4. Distance-Dependent Structure and Function of Neuronal Dendrites
    Kristen Harris; Fiscal Year: 2007
    ..along dendrites and into dendritic spines that host excitory synapses, including microtubules for transport, polyribosomes for protein synthesis, Golgi apparatus for posttranslational modifications, endosomes for membrane recycling, ..
  5. Selective Degradation of mRNA by Herpes Simplex Virus 1
    Bernard Roizman; Fiscal Year: 2010
    ..Both class A and class B mRNAs are degraded in polyribosomes. Class C includes mRNAs that are not degraded...
  6. PROBES FOR FLOW CYTOMETRY
    Zbigniew Darzynkiewicz; Fiscal Year: 1993
    ..from solid tumors; b) studies of mechanisms responsible for differential staining of rRNA in ribosomes vs polyribosomes in situ with pyronin Y, and application of this phenomenon to a new lymphocyte stimulation assay; (3) studies ..
  7. TARGETING OF HUMAN OTC TO THE MITOCHONDRIAL MATRIX
    Arthur Horwich; Fiscal Year: 1992
    ..whereby mitochondrial protein precursors, encoded in the nucleus and synthesized on cytoplasmic polyribosomes, are posttranslationally recognized via their NH2- terminal leader peptides, translocated across one or both ..
  8. Translational Regulation of Fragile X Syndrome Gene
    JAE LIM; Fiscal Year: 2006
    ..function of FMRP has not yet been elucidated, it is believed to be a RNA-binding protein that associates with polyribosomes and the rough endoplasmic reticulum...
  9. Dissecting the molecular basis of fragile X syndrome in Drosophila
    Peng Jin; Fiscal Year: 2007
    ..is a selective RNA-binding protein that forms a messenger ribonucleoprotein (mRNP) complex associating with polyribosomes. Evidence suggests that FMRP is involved in local regulation of protein synthesis at synapses...
  10. Replication Control in Fragile X Syndrome
    BARBARA BOGGS; Fiscal Year: 2004
    ..particles in the cell and it appears to specifically interact with a subset of mRNA species on translating polyribosomes; loss of functional protein then may result in translation dysregulation of these downstream genes...
  11. Translational Regulation in Adult Neural Stem Cells
    Xinyu Zhao; Fiscal Year: 2009
    ..is a selective RNA-binding protein that forms a messenger ribonucleoprotein (mRNP) complex associating with polyribosomes. FMRP regulates protein translation and the loss of FMRP leads to abnormal translation of selective mRNAs, ..
  12. Translational Regulation in Adult Neural Stem Cells
    Xinyu Zhao; Fiscal Year: 2007
    ..is a selective RNA-binding protein that forms a messenger ribonucleoprotein (mRNP) complex associating with polyribosomes. FMRP regulates protein translation and the loss of FMRP leads to abnormal translation of selective mRNAs, ..
  13. UNDERSTANDING TRAIL SENSITIVITY IN HUMAN GLIOMA
    RUSSELL PIEPER; Fiscal Year: 2007
    ..not associated with increased FLIPs mRNA levels, but with increased association of FLIPs mRNA with translating polyribosomes. FLIPs mRNA translation is in turn influenced by two arms of the Ras pathway, both activated in GBM: the Ras-..
  14. Dendritic Protein Synthesis in Hippocampal Neurons
    Erin Schuman; Fiscal Year: 2009
    ..The discovery of polyribosomes at the base of neuronal synapses suggested the possibility that proteins might be synthesized in dendrites in ..
  15. Dendritic Protein Synthesis in Hippocampal Neurons
    Erin Schuman; Fiscal Year: 2007
    ..The discovery of polyribosomes at the base of neuronal synapses suggested the possibility that proteins might be synthesized in dendrites in ..
  16. IMP Dehydrogenase
    Lizbeth Hedstrom; Fiscal Year: 2009
    ..We have recently discovered that this enzyme associates with polyribosomes, suggesting that it also plays a role in the regulation of translation...
  17. Lafora's Progressive Myoclonus Epilepsy
    Antonio Delgado Escueta; Fiscal Year: 2003
    ..specificity protein tyrosine phosphatase (ds-PTP), 38 kDa in size and cytoplasmic in location, associated with polyribosomes. Phenotype-genotype correlations and intracellular targeting with two natural mutants in exon 3 or exon 4 ..
  18. The Roles of Steel Factor in Germ Cell Behavior in the Mouse
    Christopher Wylie; Fiscal Year: 2009
    The frag ile X mental re tardation protein (FMRP) binds mRNA and micro RNA, is associated with polyribosomes, and is localized in dendrites and axons...
  19. The Roles of Steel Factor in Germ Cell Behavior in the Mouse
    Christopher C Wylie; Fiscal Year: 2010
    The frag ile X mental re tardation protein (FMRP) binds mRNA and micro RNA, is associated with polyribosomes, and is localized in dendrites and axons...
  20. REGULATION OF OVARIAN FUNCTION BY GONADOTROPIN
    JAIRAM MENON; Fiscal Year: 2001
    ..destabilization of the LHR mRNA will be facilitated by our development of an in vitro decay system employing polyribosomes from the rat ovary, in which we demonstrated hCG-induced down-regulation of LHR mRNA...
  21. UNDERSTANDING TRAIL SENSITIVITY IN HUMAN GLIOMA
    Russell O Pieper; Fiscal Year: 2010
    ..not associated with increased FLIPs mRNA levels, but with increased association of FLIPs mRNA with translating polyribosomes. FLIPs mRNA translation is in turn influenced by two arms of the Ras pathway, both activated in GBM: the Ras-..
  22. LOCALIZATION OF MRNAS AT SYNAPTIC SITES ON DENDRITES
    Oswald Steward; Fiscal Year: 2001
    ..of the synaptic site and for modifying existing synapses; 3) that the synthetic activity of synapse associated polyribosomes is regulated in part by synaptic activity...
  23. Dendritic Protein Synthesis in Hippocampal Neurons
    KAI G ZINN; Fiscal Year: 2010
    ..The discovery of polyribosomes at the base of neuronal synapses suggested the possibility that proteins might be synthesized in dendrites in ..
  24. Dissecting the molecular basis of fragile X syndrome in Drosophila
    Peng Jin; Fiscal Year: 2010
    ..is a selective RNA-binding protein that forms a messenger ribonucleoprotein (mRNP) complex associating with polyribosomes. Evidence suggests that FMRP is involved in local regulation of protein synthesis at synapses...