polyribosomes

Summary

Summary: A multiribosomal structure representing a linear array of RIBOSOMES held together by messenger RNA; (RNA, MESSENGER); They represent the active complexes in cellular protein synthesis and are able to incorporate amino acids into polypeptides both in vivo and in vitro. (From Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)

Top Publications

  1. pmc Decapping and decay of messenger RNA occur in cytoplasmic processing bodies
    Ujwal Sheth
    Department of Molecular and Cellular Biology and Howard Hughes Medical Institute, University of Arizona, Tucson, AZ 85721, USA
    Science 300:805-8. 2003
  2. ncbi RNA granules: post-transcriptional and epigenetic modulators of gene expression
    Paul Anderson
    Division of Rheumatology, Immunology and Allergy, Brigham and Women s Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA
    Nat Rev Mol Cell Biol 10:430-6. 2009
  3. pmc Movement of eukaryotic mRNAs between polysomes and cytoplasmic processing bodies
    Muriel Brengues
    Department of Molecular and Cellular Biology and Howard Hughes Medical Institute, University of Arizona, Tucson, AZ 85721 0106, USA
    Science 310:486-9. 2005
  4. pmc Identification of many microRNAs that copurify with polyribosomes in mammalian neurons
    John Kim
    Department of Molecular Biology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA
    Proc Natl Acad Sci U S A 101:360-5. 2004
  5. pmc Ribosome and transcript copy numbers, polysome occupancy and enzyme dynamics in Arabidopsis
    Maria Piques
    Max Planck Institute of Molecular Plant Physiology, Am Muehlenberg 1, Potsdam Golm, Germany
    Mol Syst Biol 5:314. 2009
  6. ncbi The three-dimensional organization of polyribosomes in intact human cells
    Florian Brandt
    Department of Molecular Structural Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
    Mol Cell 39:560-9. 2010
  7. pmc Isolation of polysome-bound mRNA from solid tissues amenable for RT-PCR and profiling experiments
    M Julieta Del Prete
    Department of Immunology, Centro de Investigaciones Biologicas, Madrid, Spain
    RNA 13:414-21. 2007
  8. pmc FMRP stalls ribosomal translocation on mRNAs linked to synaptic function and autism
    Jennifer C Darnell
    Laboratory of Molecular Neuro Oncology, The Rockefeller University, New York, NY 10065, USA
    Cell 146:247-61. 2011
  9. ncbi Stochastic theory of protein synthesis and polysome: ribosome profile on a single mRNA transcript
    Ajeet K Sharma
    Department of Physics, Indian Institute of Technology, Kanpur 208016, India
    J Theor Biol 289:36-46. 2011
  10. pmc Co-translational mRNA decay in Saccharomyces cerevisiae
    Wenqian Hu
    Center for RNA Molecular Biology, Case Western Reserve University, Cleveland, Ohio 44106, USA
    Nature 461:225-9. 2009

Detail Information

Publications267 found, 100 shown here

  1. pmc Decapping and decay of messenger RNA occur in cytoplasmic processing bodies
    Ujwal Sheth
    Department of Molecular and Cellular Biology and Howard Hughes Medical Institute, University of Arizona, Tucson, AZ 85721, USA
    Science 300:805-8. 2003
    ..These results define the flux of mRNAs between polysomes and P bodies as a critical aspect of cytoplasmic mRNA metabolism and a possible site for regulation of mRNA degradation...
  2. ncbi RNA granules: post-transcriptional and epigenetic modulators of gene expression
    Paul Anderson
    Division of Rheumatology, Immunology and Allergy, Brigham and Women s Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA
    Nat Rev Mol Cell Biol 10:430-6. 2009
    ..We propose that RNA granules can be both a cause and a consequence of altered mRNA translation, decay or editing. In this capacity, RNA granules serve as key modulators of post-transcriptional and epigenetic gene expression...
  3. pmc Movement of eukaryotic mRNAs between polysomes and cytoplasmic processing bodies
    Muriel Brengues
    Department of Molecular and Cellular Biology and Howard Hughes Medical Institute, University of Arizona, Tucson, AZ 85721 0106, USA
    Science 310:486-9. 2005
    ..Moreover, the presence of related proteins in P-bodies and maternal mRNA storage granules suggests this mechanism is widely adapted for mRNA storage...
  4. pmc Identification of many microRNAs that copurify with polyribosomes in mammalian neurons
    John Kim
    Department of Molecular Biology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA
    Proc Natl Acad Sci U S A 101:360-5. 2004
    ..Moreover, all of the miRNAs that were tested cofractionate with polyribosomes, the sites of active translation...
  5. pmc Ribosome and transcript copy numbers, polysome occupancy and enzyme dynamics in Arabidopsis
    Maria Piques
    Max Planck Institute of Molecular Plant Physiology, Am Muehlenberg 1, Potsdam Golm, Germany
    Mol Syst Biol 5:314. 2009
    ..Global translation rates are used to estimate the energy costs of protein synthesis and relate them to the plant carbon budget, in particular the rates of starch degradation and respiration at night...
  6. ncbi The three-dimensional organization of polyribosomes in intact human cells
    Florian Brandt
    Department of Molecular Structural Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
    Mol Cell 39:560-9. 2010
    ..The distinct neighbor orientations found in situ resemble configurations of bacterial polysomes in vitro, indicating a conserved supramolecular organization with implications for nascent polypeptide folding...
  7. pmc Isolation of polysome-bound mRNA from solid tissues amenable for RT-PCR and profiling experiments
    M Julieta Del Prete
    Department of Immunology, Centro de Investigaciones Biologicas, Madrid, Spain
    RNA 13:414-21. 2007
    ....
  8. pmc FMRP stalls ribosomal translocation on mRNAs linked to synaptic function and autism
    Jennifer C Darnell
    Laboratory of Molecular Neuro Oncology, The Rockefeller University, New York, NY 10065, USA
    Cell 146:247-61. 2011
    ..In addition, they provide insight into the molecular basis of the cognitive and allied defects in FXS and ASD and suggest multiple targets for clinical intervention...
  9. ncbi Stochastic theory of protein synthesis and polysome: ribosome profile on a single mRNA transcript
    Ajeet K Sharma
    Department of Physics, Indian Institute of Technology, Kanpur 208016, India
    J Theor Biol 289:36-46. 2011
    ..The theoretical framework developed here will serve in guiding further experiments and in analyzing the data to gain deep insight into various kinetic processes involved in translation...
  10. pmc Co-translational mRNA decay in Saccharomyces cerevisiae
    Wenqian Hu
    Center for RNA Molecular Biology, Case Western Reserve University, Cleveland, Ohio 44106, USA
    Nature 461:225-9. 2009
    ..The data indicate that dissociation of ribosomes from mRNA is not a prerequisite for decay and we suggest that the 5'-3' polarity of mRNA degradation has evolved to ensure that the last translocating ribosome can complete translation...
  11. pmc Yeast translational response to high salinity: global analysis reveals regulation at multiple levels
    Daniel Melamed
    Faculty of Biology, Technion Israel Institute of Technology, Haifa 32000, Israel
    RNA 14:1337-51. 2008
    ..Since a relatively small number of genes was affected by Hog1p deletion, additional signaling pathways are likely to be involved in coordinating the translational response to severe salinity stress...
  12. ncbi Ataxin-2 and its Drosophila homolog, ATX2, physically assemble with polyribosomes
    Terrence F Satterfield
    Department of Genome Sciences, University of Washington School of Medicine, Seattle, WA 98195 7730, USA
    Hum Mol Genet 15:2523-32. 2006
    ..Here, we demonstrate that ataxin-2 and its Drosophila homolog, ATX2, assemble with polyribosomes and poly(A)-binding protein (PABP), a key regulator of mRNA translation...
  13. pmc Genome-wide analysis of mRNA translation profiles in Saccharomyces cerevisiae
    Yoav Arava
    Department of Biochemistry, Stanford University, Stanford, CA 94305 5307, USA
    Proc Natl Acad Sci U S A 100:3889-94. 2003
    ..Global analysis revealed an unexpected correlation: Ribosome density decreases with increasing ORF length. Models to account for this surprising observation are discussed...
  14. pmc Polysomes, P bodies and stress granules: states and fates of eukaryotic mRNAs
    Vidya Balagopal
    Department of Molecular and Cellular Biology and Howard Hughes Medical Institute, University of Arizona, Tucson, AZ 85721 0206, USA
    Curr Opin Cell Biol 21:403-8. 2009
    ..Here we discuss how these different granules suggest a dynamic model for the metabolism of cytoplasmic mRNAs wherein they cycle between different mRNP states with different functional properties and subcellular locations...
  15. ncbi Phosphorylation influences the translation state of FMRP-associated polyribosomes
    Stephanie Ceman
    Department of Human Genetics, Emory University School of Medicine, Atlanta, GA 30322, USA
    Hum Mol Genet 12:3295-305. 2003
    ..FMRP is a nucleocytoplasmic RNA binding protein that is primarily associated with polyribosomes. FMRP is believed to be a translational repressor and may regulate the translation of certain mRNAs at the ..
  16. pmc AU-rich-element-mediated upregulation of translation by FXR1 and Argonaute 2
    Shobha Vasudevan
    Department of Molecular Biophysics and Biochemistry, Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, CT 06536, USA
    Cell 128:1105-18. 2007
    ..This novel cell-growth-dependent translation activation role for FXR1 and AGO2 allows new insights into ARE-mediated signaling and connects two important posttranscriptional regulatory systems in an unexpected way...
  17. ncbi Polysome analysis and RNA purification from sucrose gradients
    Tomas Masek
    Department of Genetics and Microbiology, Charles University in Prague, Prague, Czech Republic
    Methods Mol Biol 703:293-309. 2011
    ..We also discuss possible experimental pitfalls of data normalization, describe main alternatives of the basic protocol and outline a novel application of denaturing RNA electrophoresis in several steps of polysome profile analysis...
  18. pmc Bacterial translational regulations: high diversity between all mRNAs and major role in gene expression
    Flora Picard
    Universite de Toulouse INSA, UPS, INP LISBP, 135 Avenue de Rangueil, Toulouse, F 31077, France
    BMC Genomics 13:528. 2012
    ..To experimentally explore mRNA translational level regulation at the systemic level, the detailed translational status (translatome) of all mRNAs was measured in the model bacterium Lactococcus lactis in exponential phase growth...
  19. pmc Nonsense-mediated mRNA decapping occurs on polyribosomes in Saccharomyces cerevisiae
    Wenqian Hu
    Center for RNA Molecular Biology, Case Western Reserve University, Cleveland, Ohio, USA
    Nat Struct Mol Biol 17:244-7. 2010
    ..We show decapped nonsense-containing mRNA associate with polyribosomes, indicating that recognition and degradation are tightly coupled and that polyribosomes are major sites for ..
  20. pmc The HOG pathway dictates the short-term translational response after hyperosmotic shock
    Jonas Warringer
    Department of Cell and Molecular Biology, Lundberg Laboratory, University of Gothenburg, S 40530 Göteborg, Sweden
    Mol Biol Cell 21:3080-92. 2010
    ..Our results show the importance of translational control for fine tuning of the adaptive responses...
  21. pmc eIF4GI links nutrient sensing by mTOR to cell proliferation and inhibition of autophagy
    Francisco Ramírez-Valle
    Department of Microbiology, New York University School of Medicine, New York, NY 10016, USA
    J Cell Biol 181:293-307. 2008
    ..The majority of mRNAs that were translationally impaired with eIF4GI depletion were excluded from polyribosomes due to the presence of multiple upstream open reading frames and low mRNA abundance...
  22. ncbi Differential mRNA translation contributes to gene regulation under non-stress and dehydration stress conditions in Arabidopsis thaliana
    Riki Kawaguchi
    Department of Botany and Plant Sciences, Center for Plant Cell Biology, University of California, Riverside, CA 92521 0124, USA
    Plant J 38:823-39. 2004
    ..Thus, the abundance of PS mRNA may provide a more accurate estimate of gene expression than total cellular mRNA because of extensive differential translational regulation...
  23. pmc miRNP:mRNA association in polyribosomes in a human neuronal cell line
    Peter T Nelson
    Department of Pathology, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    RNA 10:387-94. 2004
    ..eIF2C2 (a member of the Argonaute family of proteins), Gemin3, and Gemin4 along with miRNAs cosediment with polyribosomes. Furthermore, we describe a physical association between a let-7b (miRNA)-containing miRNP and its putative ..
  24. pmc Translational reprogramming following UVB irradiation is mediated by DNA-PKcs and allows selective recruitment to the polysomes of mRNAs encoding DNA repair enzymes
    Ian R Powley
    Centre for Biomolecular Sciences, School of Pharmacy, University of Nottingham, University Park, Nottingham NG7 2RD, United Kingdom
    Genes Dev 23:1207-20. 2009
    ....
  25. pmc FMR1 transcript isoforms: association with polyribosomes; regional and developmental expression in mouse brain
    David M Brackett
    Department of Biochemistry, University of Washington, Seattle, Washington, United States of America
    PLoS ONE 8:e58296. 2013
    ..as two orders of magnitude amongst themselves in both adult and embryonic mouse brain, all are associated with polyribosomes, consistent with translation into the corresponding isoforms of the protein product, FMRP (Fragile X Mental ..
  26. pmc A network of multiple regulatory layers shapes gene expression in fission yeast
    Daniel H Lackner
    Cancer Research UK Fission Yeast Functional Genomics Group, Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK
    Mol Cell 26:145-55. 2007
    ..These rich data sets, all acquired under a standardized condition, reveal a substantial coordination between regulatory layers and provide a basis for a systems-level understanding of multilayered gene-expression programs...
  27. pmc Translation suppression promotes stress granule formation and cell survival in response to cold shock
    Sarah Hofmann
    Helmholtz Junior Research Group Posttranscriptional Control of Gene Expression, Center for Organismal Studies, Heidelberg, Germany
    Mol Biol Cell 23:3786-800. 2012
    ..Our results demonstrate that cells actively suppress protein synthesis by parallel pathways, which induce SG formation and ensure cellular survival during hypothermia...
  28. ncbi Mammalian Smaug is a translational repressor that forms cytoplasmic foci similar to stress granules
    María V Baez
    Fundacion Instituto Leloir, IIBBA CONICET and IIB Facultad de Ciencias Exactas y Naturales, University of Buenos Aires, C1405BWE Buenos Aires, Argentina
    J Biol Chem 280:43131-40. 2005
    ..Biochemical analysis indicated that mSmaug 1 is present in synaptoneurosomal 20 S particles. These results suggest a role for mammalian Smaug 1 in RNA granule formation and translation regulation in neurons...
  29. pmc Biochemical evidence for the association of fragile X mental retardation protein with brain polyribosomal ribonucleoparticles
    Edouard W Khandjian
    Unite de Recherche en Genetique Humaine et Moleculaire, Centre de recherche Hôpital Saint François d Assise, Centre Hospitalier Universitaire de Quebec, Quebec, QC, Canada G1L 3L5
    Proc Natl Acad Sci U S A 101:13357-62. 2004
    ..We demonstrate that, in the brain, FMRP is present in actively translating polyribosomes, and we show that this association is acutely sensitive to the type of detergent required to release ..
  30. ncbi Translational regulation of the human achaete-scute homologue-1 by fragile X mental retardation protein
    Michael Fähling
    Charite, Universitatsmedizin Berlin, Institut für Vegetative Physiologie, Tucholskystrasse 2, D 10117 Berlin
    J Biol Chem 284:4255-66. 2009
    ..In conclusion, we identified hASH1 as a novel downstream target of FMRP. Improved translation efficiency of hASH1 mRNA by FMRP may represent an important regulatory switch in neuronal differentiation...
  31. ncbi PKCbetaII modulates translation independently from mTOR and through RACK1
    Stefano Grosso
    Molecular Histology and Cell Growth, HSR, 20132 Milan, Italy
    Biochem J 415:77-85. 2008
    ..Taken together the results of the present study show that PKCbetaII can act as a specific PKC isoform regulating translation, in an mTOR-independent fashion, possibly close to the ribosomal machinery...
  32. pmc YbeY, a heat shock protein involved in translation in Escherichia coli
    Aviram Rasouly
    Department of Molecular Microbiology and Biotechnology, The George S Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel
    J Bacteriol 191:2649-55. 2009
    ..Taken together, the data indicate that YbeY is an important factor for bacterial translation even at 37 degrees C but becomes essential at high temperatures...
  33. pmc Genome-wide analysis of transcript abundance and translation in Arabidopsis seedlings subjected to oxygen deprivation
    Cristina Branco-Price
    Department of Botany and Plant Sciences, Center for Plant Cell Biology, University of California, Riverside, CA 92521, USA
    Ann Bot 96:647-60. 2005
    ..The aim of this study was to evaluate the role of translational regulation in response to oxygen deprivation (hypoxia)...
  34. pmc Mammalian Staufen 1 is recruited to stress granules and impairs their assembly
    María Gabriela Thomas
    Fundacion Instituto Leloir, Av Patricias Argentinas 435, Buenos Aires, Argentina
    J Cell Sci 122:563-73. 2009
    ..We propose that Stau1 is involved in recovery from stress by stabilizing polysomes, thus helping stress granule dissolution...
  35. pmc Novel zinc-responsive post-transcriptional mechanisms reciprocally regulate expression of the mouse Slc39a4 and Slc39a5 zinc transporters (Zip4 and Zip5)
    Benjamin P Weaver
    Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City, KS 66160 7421, USA
    Biol Chem 388:1301-12. 2007
    ..After zinc-gavage, ZIP5 is rapidly resynthesized and targeted to the basolateral membranes of these cell types...
  36. ncbi The lin-4 regulatory RNA controls developmental timing in Caenorhabditis elegans by blocking LIN-14 protein synthesis after the initiation of translation
    P H Olsen
    Department of Biological Sciences, Dartmouth College, Hanover, New Hampshire 03755, USA
    Dev Biol 216:671-80. 1999
    ....
  37. pmc Stress granules and processing bodies are dynamically linked sites of mRNP remodeling
    Nancy Kedersha
    Division of Rheumatology and Immunology, Harvard Medical School, Brigham and Women s Hospital, Boston, MA 02115, USA
    J Cell Biol 169:871-84. 2005
    ..We propose that mRNA released from disassembled polysomes is sorted and remodeled at SGs, from which selected transcripts are delivered to PBs for degradation...
  38. pmc Translation of a small subset of Caenorhabditis elegans mRNAs is dependent on a specific eukaryotic translation initiation factor 4E isoform
    Tzvetanka D Dinkova
    Department of Biochemistry and Molecular Biology, Louisiana State University Health Sciences Center, Shreveport, LA 71130 3932, USA
    Mol Cell Biol 25:100-13. 2005
    ..The functions of these proteins can explain some phenotypes observed in ife-4 knockout mutants. These results indicate that translation of a limited subset of mRNAs is dependent on a specific isoform of eIF4E...
  39. pmc Activated platelets mediate inflammatory signaling by regulated interleukin 1beta synthesis
    S Lindemann
    Department of Internal Medicine, University of Utah, Salt Lake City, UT 84112, USA
    J Cell Biol 154:485-90. 2001
    ....
  40. ncbi In vivo stabilization of preinitiation complexes by formaldehyde cross-linking
    LEOS VALASEK
    Institute of Microbiology, AS CR, Prague, Czech Republic
    Methods Enzymol 429:163-83. 2007
    ..In addition, different conditions for displaying the polysome profile or PIC analysis by SDC, used to address different questions, will be outlined...
  41. pmc RAS/ERK signaling promotes site-specific ribosomal protein S6 phosphorylation via RSK and stimulates cap-dependent translation
    Philippe P Roux
    Department of Pathology and Cell Biology, Institut de recherche en immunologie et en cancérologie IRIC, Universite de Montreal, Montreal, Quebec, Canada
    J Biol Chem 282:14056-64. 2007
    ..These data demonstrate that RSK provides an mTOR-independent pathway linking the Ras/ERK signaling cascade to the translational machinery...
  42. pmc Nip7p interacts with Nop8p, an essential nucleolar protein required for 60S ribosome biogenesis, and the exosome subunit Rrp43p
    N I Zanchin
    Department of Biology, University of Rochester, Rochester, New York 14627, USA
    Mol Cell Biol 19:1518-25. 1999
    ..Distinct pools of Rrp43p may interact both with the exosome and with Nip7p, possibly both in the nucleus and in the cytoplasm, to catalyze analogous reactions in the multistep process of 60S ribosome biogenesis and mRNA turnover...
  43. pmc Genome-wide analysis demonstrates conserved localization of messenger RNAs to mitotic microtubules
    Michael D Blower
    Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA
    J Cell Biol 179:1365-73. 2007
    ..laevis meiotic spindles, and a subset of microtubule-bound mRNAs (MT-mRNAs) associate with polyribosomes. Although many MT-mRNAs associate with polyribosomes, we find that active translation is not required for mRNA ..
  44. pmc Functional overlap between eIF4G isoforms in Saccharomyces cerevisiae
    Bryan K Clarkson
    Department of Molecular and Cell Biology, University of California, Berkeley, California, United States of America
    PLoS ONE 5:e9114. 2010
    ..These results suggest that total eIF4G levels, but not isoform-specific functions, determine mRNA-specific translational efficiency...
  45. ncbi Phosphorylation of eIF4E by MNKs supports protein synthesis, cell cycle progression and proliferation in prostate cancer cells
    Andrea Bianchini
    Department of Public Health and Cell Biology, University of Rome Tor Vergata, Rome, Italy
    Carcinogenesis 29:2279-88. 2008
    ....
  46. ncbi Fragile X mental retardation protein induces synapse loss through acute postsynaptic translational regulation
    Brad E Pfeiffer
    Center for Basic Neuroscience, Department of Physiology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    J Neurosci 27:3120-30. 2007
    ..K homology domain 2 (KH2) RNA-binding domain and dephosphorylation of FMRP at S500 were required for the effects of FMRP on synapse number, indicating that FMRP interaction with RNA and translating polyribosomes leads to synapse loss.
  47. ncbi The translational efficiencies of the two Leishmania infantum HSP70 mRNAs, differing in their 3'-untranslated regions, are affected by shifts in the temperature of growth through different mechanisms
    Cristina Folgueira
    Centro de Biologia Molecular Severo Ochoa, Universidad Autonoma de Madrid, 28049 Madrid, Spain
    J Biol Chem 280:35172-83. 2005
    ..Remarkably, the deltaHSP70 mutant synthesizes HSP70 at a lower rate than the wild-type parasites. Overall, our data suggest that the biological function of the HSP70-II gene is to top up HSP70 levels under conditions of stress...
  48. ncbi SmpB as the handyman of tmRNA during trans-translation
    Brice Felden
    Universite de Rennes 1, INSER M U835 UPRE S EA2311, Laboratoire de Biochimie Pharmaceutique, Rennes, France
    RNA Biol 8:440-9. 2011
    ....
  49. pmc The significance of translation regulation in the stress response
    Flora Picard
    Universite de Toulouse INSA, UPS, INP LISBP, 135 Avenue de Rangueil, F 31077, Toulouse, France
    BMC Genomics 14:588. 2013
    ....
  50. pmc Availability of Rubisco small subunit up-regulates the transcript levels of large subunit for stoichiometric assembly of its holoenzyme in rice
    Yuji Suzuki
    Graduate School of Agricultural Science, Tohoku University, Sendai 981 8555, Japan
    Plant Physiol 160:533-40. 2012
    ..These results demonstrate that the availability of RBCS protein up-regulates the gene expression of rbcL primarily at the transcript level in a quantitative manner for stoichiometric assembly of Rubisco holoenzyme...
  51. pmc The brefeldin A resistance protein Bfr1p is a component of polyribosome-associated mRNP complexes in yeast
    B D Lang
    Graduate Program in Biochemistry, Emory University School of Medicine, 1462 Clifton Road NE, Atlanta, GA 30322, USA
    Nucleic Acids Res 29:2567-74. 2001
    ..Here, we have explored this possibility further. We report that Bfr1p associates with yeast polyribosomes and mRNP complexes even in the absence of Scp160p, and that its interaction with Scp160p-containing mRNP ..
  52. pmc The essential polysome-associated RNA-binding protein RBP42 targets mRNAs involved in Trypanosoma brucei energy metabolism
    Anish Das
    Department of Microbiology and Molecular Genetics, UMDNJ NJ Medical School, Newark, New Jersey 07103, USA
    RNA 18:1968-83. 2012
    ..Although the mechanism of RBP42's function is unclear at present, we speculate that RBP42 plays a critical role in modulating T. brucei energy metabolism...
  53. ncbi Topology of mRNA chain in isolated eukaryotic double-row polyribosomes
    Zh A Afonina
    Institute of Protein Research, Russian Academy of Sciences, 142290 Pushchino, Moscow Region, Russia
    Biochemistry (Mosc) 78:445-54. 2013
    In the process of protein synthesis, the translating ribosomes of eukaryotic cells form polyribosomes that are found to be multiplex functional complexes possessing elements of ordered spatial organization...
  54. ncbi TOPs and their regulation
    T L Hamilton
    School of Pharmacy, University of Nottingham, University Park, Nottingham, NG7 2RD, UK
    Biochem Soc Trans 34:12-6. 2006
    ....
  55. pmc Reactivation of stalled polyribosomes in synaptic plasticity
    Tyson E Graber
    Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, QC, Canada, H3A 2B4
    Proc Natl Acad Sci U S A 110:16205-10. 2013
    ..in situ evidence supporting an alternative model in which synaptic mRNAs are transported as stably paused polyribosomes. Remarkably, we show that metabotropic glutamate receptor activation allows the synthesis of proteins that ..
  56. ncbi Posttranscriptional mechanisms involved in the control of expression of the stage-specific GP82 surface glycoprotein in Trypanosoma cruzi
    Luciana Girotto Gentil
    Department of Microbiology, Immunology and Parasitology, Escola Paulista de Medicina, UNIFESP, Rua Botucatu, Sao Paulo, Brazil
    Acta Trop 109:152-8. 2009
    ..Consistent with this finding, northern blot analysis showed that GP82 mRNAs were mobilized to polysomes and consequently translated, but only in metacyclic trypomastigotes...
  57. ncbi Acidic stress induces the formation of P-bodies, but not stress granules, with mild attenuation of bulk translation in Saccharomyces cerevisiae
    Aya Iwaki
    Laboratory of Microbial Technology, Graduate School of Science and Technology, Kyoto Institute of Technology, Matsugasaki, Kyoto 606 8585, Japan
    Biochem J 446:225-33. 2012
    ....
  58. pmc Characterization of Staufen 1 ribonucleoprotein complexes
    Cornelia Brendel
    Institute for Cell Biochemistry and Clinical Neurobiology, University Hospital Hamburg Eppendorf, D 20246 Hamburg, Germany
    Biochem J 384:239-46. 2004
    ....
  59. ncbi The antidepressant sertraline inhibits translation initiation by curtailing mammalian target of rapamycin signaling
    Chen Ju Lin
    Department of Biochemistry and Goodman Cancer Center, McGill University, Montreal, Quebec, Canada
    Cancer Res 70:3199-208. 2010
    ..Our results indicate that sertraline exerts antiproliferative activity by targeting the mTOR signaling pathway in a REDD1-dependent manner...
  60. ncbi Relationship between yeast polyribosomes and Upf proteins required for nonsense mRNA decay
    A L Atkin
    School of Biological Sciences, University of Nebraska, Lincoln, Nebraska 68588 0666, USA
    J Biol Chem 272:22163-72. 1997
    ..of soluble lysates and found that all three proteins co-distributed with 80 S ribosomal particles and polyribosomes. Treatment of lysates with RNase A caused a coincident collapse of polyribosomes and each Upf protein into ..
  61. pmc P-body formation is a consequence, not the cause, of RNA-mediated gene silencing
    Ana Eulalio
    Max Planck Institute for Developmental Biology, Spemannstrasse 35, D 72076 Tubingen, Germany
    Mol Cell Biol 27:3970-81. 2007
    ..Thus, even though P-body components play crucial roles in mRNA silencing and decay, aggregation into P bodies is not required for function but is instead a consequence of their activity...
  62. ncbi Shape of large bound polysomes in cultured fibroblasts and thyroid epithelial cells
    A K Christensen
    Department of Anatomy and Cell Biology, The University of Michigan Medical School, Ann Arbor 48109 0616, USA
    Anat Rec 255:116-29. 1999
    ..One or two dense particles were sometimes seen in the center of spiral or circular polysomes. The consistent hairpin shape of most large bound polysomes observed in this study suggests that their shape is quite stable...
  63. ncbi FMRP associates with polyribosomes as an mRNP, and the I304N mutation of severe fragile X syndrome abolishes this association
    Y Feng
    Howard Hughes Medical Institute, Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    Mol Cell 1:109-18. 1997
    ..We show here that normal FMRP associates with elongating polyribosomes via large mRNP particles...
  64. ncbi Polyribosomes are increased in spines of CA1 dendrites 2 h after the induction of LTP in mature rat hippocampal slices
    Jennifer N Bourne
    Synapses and Cognitive Neuroscience Center, Medical College of Georgia, Augusta, Georgia, USA
    Hippocampus 17:1-4. 2007
    Enduring long-term potentiation (LTP) requires immediate protein synthesis, hence we assessed whether more polyribosomes are present in dendritic spines of mature hippocampal dendrites after the induction of LTP...
  65. pmc Fear and safety learning differentially affect synapse size and dendritic translation in the lateral amygdala
    Linnaea E Ostroff
    Center for Neural Science, New York University, New York, NY 10003, USA
    Proc Natl Acad Sci U S A 107:9418-23. 2010
    ..There were more polyribosomes and multivesicular bodies throughout the dendrites from fear conditioned rats, indicating increases in both ..
  66. pmc IMP dehydrogenase type 1 associates with polyribosomes translating rhodopsin mRNA
    Sarah E Mortimer
    Department of Biochemistry, Brandeis University, Waltham, Massachusetts 02454 9110, USA
    J Biol Chem 283:36354-60. 2008
    ..Here we show that both IMPDH type 1 (IMPDH1) and IMPDH type 2 are associated with polyribosomes, suggesting that these housekeeping proteins have an unanticipated role in translation regulation...
  67. pmc Translation of aberrant mRNAs lacking a termination codon or with a shortened 3'-UTR is repressed after initiation in yeast
    Toshifumi Inada
    Department of Molecular Biology, Graduate School of Science, Nagoya University, Nagoya, Japan
    EMBO J 24:1584-95. 2005
    ..We also found that translation of aberrant mRNAs with a shortened 3'-UTR was repressed, suggesting that an improper spatial distance between the termination codon and the 3' end of mRNA results in translation repression...
  68. pmc Chloroplast biogenesis of photosystem II cores involves a series of assembly-controlled steps that regulate translation
    Limor Minai
    Unité Mixte de Recherche 7141, Centre National de la Recherche Scientifique Université Pierre et Marie Curie Paris VI, Institut de Biologie Physico Chimique, France
    Plant Cell 18:159-75. 2006
    ..De novo synthesis and repair of photosystem II complexes are independently controlled...
  69. pmc Aldosterone and vasopressin affect {alpha}- and {gamma}-ENaC mRNA translation
    Andrea Perlewitz
    Institut für Vegetative Physiologie, Universitatsmedizin Berlin Charite, D 10117 Berlin, Germany
    Nucleic Acids Res 38:5746-60. 2010
    ..Immunoprecipitation and UV-crosslinking analysis of γ-ENaC-mRNA/HuR complexes document the significance of γ-ENaC-mRNA-3'-UTR/HuR interaction for hormonal control of ENaC synthesis...
  70. pmc Inhibition of polysome assembly enhances imatinib activity against chronic myelogenous leukemia and overcomes imatinib resistance
    Min Zhang
    Division of Hematology Oncology, Department of Medicine, University of California at Irvine, Irvine, California 92697, USA
    Mol Cell Biol 28:6496-509. 2008
    ..Together, this work supports the inhibition of translation initiation as a therapeutic strategy for treating cancers fueled by dysregulated translation...
  71. ncbi Developmental expression of the fragile X-related 1 proteins in mouse testis: association with microtubule elements
    M E Huot
    , , 10, rue de l'Espinay, , Canada
    Hum Mol Genet 10:2803-11. 2001
    ..Since the cytoskeletal framework is implicated in cellular plasticity as well as in mRNA transport, we propose new possibilities for the function(s) of the FXR proteins...
  72. pmc Immunopurification of polyribosomal complexes of Arabidopsis for global analysis of gene expression
    Maria Eugenia Zanetti
    Department of Botany and Plant Science, Center for Plant Cell Biology, University of California, Riverside, California 92521 0124, USA
    Plant Physiol 138:624-35. 2005
    Immunoaffinity purification of polyribosomes (polysomes) from crude leaf extracts of Arabidopsis (Arabidopsis thaliana) was achieved with transgenic genotypes that overexpress a translational fusion of a ribosomal protein (RP) with a His(..
  73. pmc The RACK1 signal anchor protein from Trypanosoma brucei associates with eukaryotic elongation factor 1A: a role for translational control in cytokinesis
    Sandesh Regmi
    Department of Biological Sciences, Southern Methodist University, Dallas, TX 75275, USA
    Mol Microbiol 70:724-45. 2008
    ..The synergy between anisomycin and TbRACK1 RNAi suggests that continued translation is required for complete ingression of the cleavage furrow...
  74. ncbi Novel isoforms of the fragile X related protein FXR1P are expressed during myogenesis
    E W Khandjian
    Unite de Recherche en Genetique Humaine et Moleculaire, Pavillon Saint François d Assise du CHUQ, Département de Biologie médicale, Faculte de Medecine, Universite Laval, Quebec, Canada
    Hum Mol Genet 7:2121-8. 1998
    ..in culture as well as in brain extracts, both P78and P70isoforms are associated with mRNPs within translating polyribosomes, similarly to their closely related FMRP homologues...
  75. ncbi Global translational responses to oxidative stress impact upon multiple levels of protein synthesis
    Daniel Shenton
    Faculty of Life Sciences, University of Manchester, Manchester M13 9PT, United Kingdom
    J Biol Chem 281:29011-21. 2006
    ..In summary, oxidative stress elicits complex translational reprogramming that is fundamental for adaptation to the stress...
  76. ncbi mRNA escape from stress granule sequestration is dictated by localization to the endoplasmic reticulum
    H Unsworth
    Department of Oncology, Imperial College London, Hammersmith Hospital Campus, Du Cane Road, London W12 0NN, UK
    FASEB J 24:3370-80. 2010
    ..These findings have important implications for cell survival given that many membrane proteins, which are translated at the ER, have important roles in detoxification...
  77. pmc Hypusine-containing protein eIF5A promotes translation elongation
    Preeti Saini
    Laboratory of Gene Regulation and Development, NICHD, National Institutes of Health, Bethesda, Maryland 20892, USA
    Nature 459:118-21. 2009
    ..Because eIF5A is a structural homologue of the bacterial protein EF-P, we propose that eIF5A/EF-P is a universally conserved translation elongation factor...
  78. ncbi A functional RNAi screen links O-GlcNAc modification of ribosomal proteins to stress granule and processing body assembly
    Takbum Ohn
    Division of Rheumatology, Immunology and Allergy, Brigham and Women s Hospital, Harvard Medical School, 1 Jimmy Fund Way, Boston, Massachusetts 02115, USA
    Nat Cell Biol 10:1224-31. 2008
    ..The lack of enzymes of the hexosamine biosynthetic pathway in budding yeast may contribute to differences between mammalian SGs and related yeast EGP (eIF4E, 4G and Pab1 containing) bodies...
  79. ncbi Evidence that microRNAs are associated with translating messenger RNAs in human cells
    Patricia A Maroney
    Center for RNA Molecular Biology and Department of Biochemistry, Case Western Reserve University School of Medicine, W127 10900 Euclid Avenue, Cleveland, Ohio 44106 4973, USA
    Nat Struct Mol Biol 13:1102-7. 2006
    ..Several lines of evidence indicate that most of these mRNAs, including a known miRNA-regulated target (KRAS mRNA), are actively being translated...
  80. pmc Stress-activated protein kinase pathway functions to support protein synthesis and translational adaptation in response to environmental stress in fission yeast
    Isabelle Dunand-Sauthier
    MRC Radiation and Genome Stability Unit, Harwell, Didcot, United Kingdom, and Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis, USA
    Eukaryot Cell 4:1785-93. 2005
    ..These findings suggest a role for the SAPK pathway in supporting translation initiation and facilitating adaptation to environmental stress in part through reducing eIF2alpha phosphorylation in fission yeast...
  81. pmc Amino acid-induced translation of TOP mRNAs is fully dependent on phosphatidylinositol 3-kinase-mediated signaling, is partially inhibited by rapamycin, and is independent of S6K1 and rpS6 phosphorylation
    H Tang
    Department of Biochemistry, The Hebrew University-Hadassah Medical School, Jerusalem 91120, Israel
    Mol Cell Biol 21:8671-83. 2001
    ....
  82. ncbi Plant stress granules and mRNA processing bodies are distinct from heat stress granules
    Christian Weber
    Department of Molecular Cell Biology, Johann Wolfgang Goethe University Frankfurt, Max von Laue Strasse 9, 60438 Frankfurt, Germany
    Plant J 56:517-30. 2008
    ..SGs and PBs can be clearly discriminated from the previously described heat stress granules (HSGs), which evidently do not contain mRNPs. Thus, the role of HSGs as putative mRNP storage sites must be revised...
  83. ncbi mRNPs, polysomes or granules: FMRP in neuronal protein synthesis
    Francesca Zalfa
    Dipartimento di Biologia, Universita di Roma Tor Vergata, Via della Ricerca Scientifica 1, 00133, Roma, Italy
    Curr Opin Neurobiol 16:265-9. 2006
    ....
  84. ncbi The Shwachman-Bodian-Diamond syndrome associated protein interacts with HsNip7 and its down-regulation affects gene expression at the transcriptional and translational levels
    Cédric Hesling
    Center for Structural Molecular Biology, Brazilian Synchrotron Light Laboratory, LNLS Rua Giuseppe Maximo Scolfaro 10000, PO Box 6192, CEP 13084 971, Campinas, SP, Brazil
    Exp Cell Res 313:4180-95. 2007
    ..These results indicate that SBDS is required for accurate expression of genes important for proper brain, skeletal, and blood cell development...
  85. ncbi Fragile X mental retardation protein shifts between polyribosomes and stress granules after neuronal injury by arsenite stress or in vivo hippocampal electrode insertion
    Soong Ho Kim
    Beckman Institute, University of Illinois at Urbana Champaign, Urbana, Illinois 61801, USA
    J Neurosci 26:2413-8. 2006
    ..by virtue of their TIA-1 (T-cell intracellular antigen 1) protein component, and found that FMRP moved out of polyribosomes and into SGs subsequent to oxidative stress...
  86. pmc Why Dom34 stimulates growth of cells with defects of 40S ribosomal subunit biosynthesis
    Arpita Bhattacharya
    Department of Cell Biology, Albert Einstein College of Medicine, 1300 Morris Avenue, Bronx, NY 10461, USA
    Mol Cell Biol 30:5562-71. 2010
    ....
  87. ncbi Gene expression analyzed by high-resolution state array analysis and quantitative proteomics: response of yeast to mating pheromone
    Vivian L MacKay
    Department of Biochemistry, University of Washington, Seattle, WA 98195, USA
    Mol Cell Proteomics 3:478-89. 2004
    ..These observations underscore that analysis of transcript level, albeit extremely important, is insufficient by itself to describe completely the phenotypes of cells under different conditions...
  88. ncbi A plant viral "reinitiation" factor interacts with the host translational machinery
    H S Park
    Friedrich Miescher-Institute, P.O. Box 2543, CH-4002, Basel, Switzerland
    Cell 106:723-33. 2001
    ....
  89. ncbi Isolation of plant polysomal mRNA by differential centrifugation and ribosome immunopurification methods
    Angelika Mustroph
    Center for Plant Cell Biology and Department of Botany and Plant Sciences, University of California, Riverside, CA, USA
    Methods Mol Biol 553:109-26. 2009
    b>Polyribosomes (polysomes) form as multiple ribosomes engage in translation on a single mRNA. This process is regulated for individual mRNAs by both development and the environment...
  90. ncbi Trapping of messenger RNA by Fragile X Mental Retardation protein into cytoplasmic granules induces translation repression
    Rachid Mazroui
    Unite de Recherche en Genetique Humaine et Moleculaire, Centre de recherche Hôpital Saint François d Assise, 10 rue de l Espinay, Quebec G1L 3L5, PQ, Canada
    Hum Mol Genet 11:3007-17. 2002
    ..The FMRP-containing mRNPs are dynamic structures that oscillate between polyribosomes and cytoplasmic granules reminiscent of the Stress Granules that contain repressed mRNAs...
  91. ncbi Analysis of polysomal mRNA populations of mouse oocytes and zygotes: dynamic changes in maternal mRNA utilization and function
    Santhi Potireddy
    The Department of Biochemistry, Temple University School of Medicine, Philadelphia, PA 19140, USA
    Dev Biol 298:155-66. 2006
    ..We observe global transitions in the functional classes of translated MmRNAs and apparent changes in the underlying cis-regulatory mechanisms...
  92. ncbi Selective mRNA translation coordinates energetic and metabolic adjustments to cellular oxygen deprivation and reoxygenation in Arabidopsis thaliana
    Cristina Branco-Price
    Center for Plant Cell Biology, University of California, Riverside, CA 92521, USA
    Plant J 56:743-55. 2008
    ..Hypoxia stress and reoxygenation promoted adjustments in the levels of polyribosomes (polysomes) that were highly coordinated with cellular ATP content...
  93. pmc Staufen recruitment into stress granules does not affect early mRNA transport in oligodendrocytes
    María G Thomas
    Fundacion Instituto Leloir, IIB Facultad de Ciencias Exactas y Naturales, University of Buenos Aires, IIBBA CONICET, Buenos Aires, Argentina
    Mol Biol Cell 16:405-20. 2005
    ....
  94. ncbi Gonadotropin-regulated testicular RNA helicase (GRTH/Ddx25) is a transport protein involved in gene-specific mRNA export and protein translation during spermatogenesis
    Yi Sheng
    Section on Molecular Endocrinology, Endocrinology and Reproduction Research Branch, NICHD, National Institutes of Health, Bethesda, Maryland 20892 4510, USA
    J Biol Chem 281:35048-56. 2006
    ..The phosphorylated cytoplasmic 61-kDa species was associated with polyribosomes. Confocal studies on COS-1 cells showed that GRTH-GFP was retained in the nucleus by treatment with a RNA ..
  95. pmc A novel function for fragile X mental retardation protein in translational activation
    Elias G Bechara
    Institut de Pharmacologie Moleculaire et Cellulaire, Valbonne, France
    PLoS Biol 7:e16. 2009
    ....
  96. pmc RelA protein stimulates the activity of RyhB small RNA by acting on RNA-binding protein Hfq
    Liron Argaman
    Department of Microbiology and Molecular Genetics, Institute for Medical Research Israel Canada, Hebrew University Hadassah Medical School, Jerusalem 91120, Israel
    Proc Natl Acad Sci U S A 109:4621-6. 2012
    ....
  97. ncbi Saccharomyces cerevisiae Gis2 interacts with the translation machinery and is orthogonal to myotonic dystrophy type 2 protein ZNF9
    Morgan A Sammons
    Department of Biological Sciences, Vanderbilt University, Nashville, TN 37232, USA
    Biochem Biophys Res Commun 406:13-9. 2011
    ..These data suggest that Gis2 is functionally orthologous to ZNF9 and acts as a cap-independent translation factor...
  98. ncbi Global and specific translational control by rapamycin in T cells uncovered by microarrays and proteomics
    Annabelle Grolleau
    Department of Microbiology and Immunology, University of Michigan, Ann Arbor, Michigan 48109, USA
    J Biol Chem 277:22175-84. 2002
    ....
  99. pmc Expression profiling reveals meiotic male germ cell mRNAs that are translationally up- and down-regulated
    Naoko Iguchi
    Center for Research on Reproduction and Women s Health, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Proc Natl Acad Sci U S A 103:7712-7. 2006
    ..This high-throughput approach defines the changing translation patterns of populations of genes as male germ cells differentiate and identifies groups of meiotic transcripts that are translationally up- and down-regulated...
  100. ncbi Akt phosphorylation of La regulates specific mRNA translation in glial progenitors
    F Brenet
    Department of Cancer Biology and Genetics, Memorial Sloan Kettering Cancer Center, New York, NY, USA
    Oncogene 28:128-39. 2009
    ..Therefore, La appears to be an important contributor to Akt-mediated translational regulation of these transcripts in murine glial cells...
  101. ncbi Heme-regulated inhibitor kinase-mediated phosphorylation of eukaryotic translation initiation factor 2 inhibits translation, induces stress granule formation, and mediates survival upon arsenite exposure
    Edward McEwen
    Howard Hughes Medical Institute and the Department of Biological Chemistry, University of Michigan Medical Center, Ann Arbor 48109, USA
    J Biol Chem 280:16925-33. 2005
    ..The results demonstrated an essential role for HRI in mediating arsenite stress-induced phosphorylation of eukaryotic translation initiation factor 2alpha, inhibition of protein synthesis, stress granule formation, and survival...

Research Grants62

  1. Distance-Dependent Structure and Function of Neuronal Dendrites
    Kristen M Harris; Fiscal Year: 2013
    ..microtubules for transport;smooth endoplasmic reticulum for calcium regulation and protein trafficking;polyribosomes, Golgi outposts, and spine apparatuses for local protein synthesis;recycling and sorting endosomes for ..
  2. Distance-Dependent Structure and Function of Neuronal Dendrites
    Kristen M Harris; Fiscal Year: 2010
    ..along dendrites and into dendritic spines that host excitory synapses, including microtubules for transport, polyribosomes for protein synthesis, Golgi apparatus for posttranslational modifications, endosomes for membrane recycling, ..
  3. RiboTag: A novel technique to profile cell type specific gene expression and inv
    GEORGE STANLEY MCKNIGHT; Fiscal Year: 2013
    ..The polyribosomes containing the transcribed and translatable mRNAs are isolated by immunological techniques and then the RNA is ..
  4. Translational Regulation of Mosquito mRNAs during Plasmodium Infection
    Jinsong Zhu; Fiscal Year: 2010
    ..We will use sucrose density gradient centrifugation to separate mRNAs associated with polyribosomes from those that are translationally inactive...
  5. Translational Regulation of Adult Neural Stem Cells
    Xinyu Zhao; Fiscal Year: 2013
    ..X mental retardation protein (FMRP) is a neuron- enriched selective RNA-binding protein associated with polyribosomes and it is known to regulate protein translation...
  6. Dissecting the molecular basis of fragile X syndrome in Drosophila
    Peng Jin; Fiscal Year: 2011
    ..is a selective RNA-binding protein that forms a messenger ribonucleoprotein (mRNP) complex associating with polyribosomes. Evidence suggests that FMRP is involved in local regulation of protein synthesis at synapses...
  7. Hepatitis C Virus and Hepatocellular Carcinoma
    Srikanta Dash; Fiscal Year: 2013
    ..Each agent inhibits translation by preventing the polyribosomes load to the HCV IRES mRNA using two separate mechanisms...
  8. Deconvoluting microRNA expression in brain
    Richard H Goodman; Fiscal Year: 2013
    ..idea that microRNAs contribute to axonal growth has received less attention, in part because the existence of polyribosomes in this compartment has been controversial...
  9. MAP Kinase Signaling in Lymphoma: A Novel Therapeutic Paradigm
    Ronald B Gartenhaus; Fiscal Year: 2013
    ..Significantly, MCT-1 has been shown by us to alter loading of mRNAs on polyribosomes (translational profile) in lymphoma cells...
  10. The Roles of Steel Factor in Germ Cell Behavior in the Mouse
    Christopher C Wylie; Fiscal Year: 2010
    ..We will test the hypothesis that signals from adjacent tissues activate expression of Steel factor in cells immediately around the germ cells at the gastrula stage. ..
  11. Selective Degradation of mRNA by Herpes Simplex Virus 1
    Bernard Roizman; Fiscal Year: 2013
    ..Both class A and class B mRNAs are degraded in polyribosomes. Class C includes mRNAs that are not degraded...
  12. UNDERSTANDING TRAIL SENSITIVITY IN HUMAN GLIOMA
    Russell O Pieper; Fiscal Year: 2010
    ..not associated with increased FLIPs mRNA levels, but with increased association of FLIPs mRNA with translating polyribosomes. FLIPs mRNA translation is in turn influenced by two arms of the Ras pathway, both activated in GBM: the Ras-..
  13. MOLECULAR INTERACTIONS OF MYELOID CELLS WITH ENDOTHELIUM
    Guy A Zimmerman; Fiscal Year: 2013
    ..of transcripts that code for critical inflammatory proteins and can be identified by their association with polyribosomes. The studies will resolve critical gaps in our knowledge of gene regulation in inflammatory systems, have ..
  14. Dendritic Protein Synthesis in Hippocampal Neurons
    KAI G ZINN; Fiscal Year: 2011
    ..The discovery of polyribosomes at the base of neuronal synapses suggested the possibility that proteins might be synthesized in dendrites in ..
  15. PROPOSED RAT MODEL OF OLIVOPONTOCEREBELLAR ATROPHY
    CHERYL GUYER; Fiscal Year: 2000
    ..of tangled microtubules, such as those described in human disease (38, 40), or exaggerated amounts of polyribosomes. Pedigrees of these rats have been established by cross-intercross and cross-backcross breeding schemes...
  16. Translational Regulation of Fragile X Syndrome Gene
    JAE LIM; Fiscal Year: 2006
    ..function of FMRP has not yet been elucidated, it is believed to be a RNA-binding protein that associates with polyribosomes and the rough endoplasmic reticulum...
  17. TARGETING PROTEINS TO CANDIDA TROPICALIS PEROXISOMES
    PAUL LAZAROW; Fiscal Year: 1990
    ..and chloroplasts in that the organelles import posttranslationally proteins that are synthesized on free polyribosomes. Peroxisomes differ in that their proteins generally lack cleavable transit peptides, whereas most ..
  18. BIOCHEMISTRY OF LATE MITOTIC EVENTS IN CULTURED CELLS
    LON HODGE; Fiscal Year: 1980
    ..Our recent information indicates that the disaggregation of polyribosomes during mitosis results in the release of free mRNP particles free of ribosomes...
  19. BIOGENESIS OF RAT LIVER PEROXISOMES
    PAUL LAZAROW; Fiscal Year: 1993
    ..and chloroplasts in that the organelles import posttranslationally proteins that are synthesized on free polyribosomes. Peroxisomes differ in that their proteins generally lack leavable transit peptides, whereas most ..
  20. PROTEIN PRODUCTION DURING SPROUTING AND SYNAPTOGENESIS
    Linda Phillips; Fiscal Year: 1990
    ..into protein increases over the denervated dentate neuropil concurrent with synapse formation, 2) most of the polyribosomes in the dentate neuropil are associated with dendritic spines, 3) the number of spine associated polyribosomes ..
  21. Replication Control in Fragile X Syndrome
    BARBARA BOGGS; Fiscal Year: 2004
    ..particles in the cell and it appears to specifically interact with a subset of mRNA species on translating polyribosomes; loss of functional protein then may result in translation dysregulation of these downstream genes...
  22. INSULIN AMINO ACID POOLS AND MUSCLE PROTEIN SYNTHESIS
    Robert Low; Fiscal Year: 1980
    ..Peptidyl-tRNA will be isolated from total and myosin-synthesizing polyribosomes and its radiolabeled amino acid specific activity compared with those of tRNA-bound, intracellular and ..
  23. Lafora's Progressive Myoclonus Epilepsy
    Antonio Delgado Escueta; Fiscal Year: 2003
    ..specificity protein tyrosine phosphatase (ds-PTP), 38 kDa in size and cytoplasmic in location, associated with polyribosomes. Phenotype-genotype correlations and intracellular targeting with two natural mutants in exon 3 or exon 4 ..
  24. Dissecting the molecular basis of fragile X syndrome in Drosophila
    Peng Jin; Fiscal Year: 2010
    ..is a selective RNA-binding protein that forms a messenger ribonucleoprotein (mRNP) complex associating with polyribosomes. Evidence suggests that FMRP is involved in local regulation of protein synthesis at synapses...
  25. ADHERENCE REGULATED MONOCYTE GENES
    JOHN HASKILL; Fiscal Year: 1999
    ..the hRNP's and a 37 kD protein, AUF1 which is able to initiate RNA degradation in vitro when associated with polyribosomes. We have used an ARE probe from the 3'UTR of GRO to assess the number and activity of proteins constitutively ..
  26. IMMUNOGLOBULIN-RELATED T LYMPHOCYTE SURFACE RECEPTORS
    JOHN MARCHALONIS; Fiscal Year: 1992
    ..We will use our specific antisera to isolate intact polyribosomes bearing nascent polypeptide chains from our cell lines, isolate mRNA and produce cDNA followed by cloning and ..
  27. BONE COLLAGEN PRODUCTION BY BONE-DERIVED TGFB
    Michael Centrella; Fiscal Year: 1992
    ..initiation complexes by hybridization and specific filter binding assays, and the entry of collagen mRNA into polyribosomes by velocity sedimentation analysis...
  28. ONTOGENY OF STRUCTURE & FUNCTION AT HIPPOCAMPAL SYNAPSES
    Kristen Harris; Fiscal Year: 2005
    ..Local protein synthesis, indicated by polyribosomes or membrane recycling, indicated by endosomes, will be evaluated to determine whether these processes could ..
  29. PROTEIN SYNTHESIS MOLECULAR ACTIVATION IN EGG & EMBRYO
    MERRIL HILLE; Fiscal Year: 1980
    ..ribosomes are made during oogenesis and stored in the egg in some unknown manner, ready for recruitment into polyribosomes within ten minutes after fertilization...
  30. 3-DIMENSIONAL STRUCTURE AND FUNCTION OF SYNAPSES
    Kristen Harris; Fiscal Year: 2002
    ..In addition, use the new tools to evaluate whether local protein synthesis, indicated by polyribosomes, or membrane recycling, indicated by endosomes, mediate the structural changes at synapses that have undergone ..
  31. Post-transcriptional regulation of TNF-alpha production
    Paul Anderson; Fiscal Year: 2005
    ..These studies might also identify molecular targets for the development of orally available TNFa blockers that can be used to treat patients with rheumatoid arthritis. ..
  32. Characterization of mammalian stress granules
    Paul J Anderson; Fiscal Year: 2010
    ..These studies have implications for our understanding of stress-regulated pathological conditions, including Alzheimer's disease, diabetes and cancer. ..
  33. ROLE OF PSGL 1 IN NATURAL KILLER CELL FUNCTION
    Paul Anderson; Fiscal Year: 2003
    ..Consequently, the overall goal of the proposed experiments is to determine how PEN5:L-selectin interactions influence NK cell function. ..
  34. The role of FAST in immune-mediated inflammatory disease
    Paul Anderson; Fiscal Year: 2008
    ..Finally, we will generate transgenic mice that overexpress FAST and mutant mice that lack FAST to determine its importance in the initiation and/or propagation of immune-mediated inflammatory disease. ..
  35. HBF VARIANTS FOR GENE THERAPY OF SICKLE CELL DISEASE
    Kazuhiko Adachi; Fiscal Year: 2005
    ..chains assemble with intermediately folded nascent gamma-chains prior to or soon after the release from polyribosomes. (ii) The amino acids at G-10, 14 and 18, which have been shown by x-ray crystallographic analysis to be at ..
  36. A novel embryonic poly(A) binding protein
    Michael Sheets; Fiscal Year: 2004
    ....
  37. DOES PKU PROTECT AGAINST CANCER?
    Neil Sidell; Fiscal Year: 2003
    ....
  38. REGULATION OF CD154 EXPRESSION
    WILLIAM FREDERICK CARSON RIGBY; Fiscal Year: 2010
    ..Importantly, the CD153'UTR CA repeats are polymorphic with certain alleles associated with CD154 overexpression and the development of RA and SLE. ..
  39. Regulation of CD154 gene expression in vivo
    William Rigby; Fiscal Year: 2002
    ..As a result, we will create a model system with which to study its unique regulation and possibly develop novel approaches to regulating its biosynthesis. ..
  40. Novel Targets of the Von Hippel Lindau Gene
    William Rigby; Fiscal Year: 2006
    ..We hypothesize that the absence of pVHL results in the activation of the p38 SAPK pathway and hnRNP A2 overexpression and propose to address this and its relevance to GLUT1 mRNA turnover. ..
  41. RIBOSOMAL GENES OF YEAST AND THEIR REGULATION
    Jonathan R Warner; Fiscal Year: 2010
    ....
  42. Beta-globin mRNA decay in erythroid cells
    Daniel Schoenberg; Fiscal Year: 2005
    ..The long-term goal is to identify new therapeutic targets for treating beta-thalassemia and other diseases caused by PTCs by better understanding the enzymatic mechanisms responsible for the degradation of PTC-containing mRNAs. ..
  43. Environmental Toxicology Using Transgenic Models
    Glen Andrews; Fiscal Year: 2005
    ..Functions of MTF-l during development will be examined in Zebrafish using morpholino antisense oligonucleotides, whole mount in situ hybridization and morphometric analyses of developing embryos. ..
  44. Myocardial Protein Synthesis After Thermal Injury
    Charles Lang; Fiscal Year: 2005
    ..Overall, the research will elucidate the mechanisms by which myocardial protein synthesis is reduced after thermal injury, leading to the better understanding and treatment of the resulting cardiomyopathy. - ..
  45. RhoB Signaling in Cancer Suppression and Cell Suicide
    George Prendergast; Fiscal Year: 2007
    ..Characterization of these signaling pathways offers a unique opportunity to obtain new insights into cancer pathophysiology and therapeutic response. ..
  46. Towards a Dendritic Proteome
    Erin Schuman; Fiscal Year: 2007
    ..The changes in dendritic proteins that underly the synaptic modifications associated with plasticity, including addiction, can be elucidated using this technique. [unreadable] [unreadable]..
  47. Control of TGF-beta Activity in Bone
    Michael Centrella; Fiscal Year: 2007
    ..Due to the importance of TGF-beta in bone metabolism, studies like these that initiate with TbetaR expression on osteoblasts may continue to define new molecular targets that could be used to focus and enhance bone growth and repair. ..
  48. AUTOIMMUNITY AND CONNECTIVE TISSUE BIOLOGY TRAINING
    William Rigby; Fiscal Year: 2008
    ..These forums facilitate the high level of faculty-student interaction that is traditional at Dartmouth and results in the recognition of our research efforts at both the national and international level. [unreadable] [unreadable]..
  49. Mechanisms directing oncoprotein and cytokine mRNA decay
    GERALD WILSON; Fiscal Year: 2008
    ..Together, these studies will further our understanding of the relationships between ARE structure, trans-factor recognition, and the cellular functions of resulting RNP complexes. ..
  50. Cleveland Cell Biology Symposium: Regulation
    ALAN TARTAKOFF; Fiscal Year: 2004
    ..The results should be an intense synthesis of knowledge which will be stimulating for both students and established faculty. ..
  51. Function of QKI RNA-binding Protein in CNS Myelination
    Yue Feng; Fiscal Year: 2006
    ..In addition, understanding how QKI regulates its RNA targets during myelination may elucidate common mechanisms for other STARs in cell growth and tumorigenesis. ..
  52. Postdoctoral Training Program in Neural Regeneration
    Oswald Steward; Fiscal Year: 2005
    ....
  53. FMRP-mediated translation regulation in neuronal development
    YUE contact FENG; Fiscal Year: 2010
    The frag ile X mental re tardation protein (FMRP) binds mRNA and micro RNA, is associated with polyribosomes, and is localized in dendrites and axons...
  54. Summer Training Program in Neuroscience
    Oswald Steward; Fiscal Year: 2006
    ..Where appropriate, trainees will be encouraged to present their results at national meetings, and support will be provided for students to attend such meetings. [unreadable] [unreadable]..
  55. Control of Spemann's organizer
    Michael Sheets; Fiscal Year: 2007
    ..We will address our view by examining the role(s) of FGF signaling pathway(s) within the organizer and the transcription of chordin as they relate to the ultimate inducing functions of Spemann's organizer. [unreadable] [unreadable]..
  56. METALLOTHIONEIN IN REPRODUCTION AND DEVELOPMENT
    Glen Andrews; Fiscal Year: 2002
    ..A testable hypothesis is that misexpression of MT will alter metal homeostasis during pregnancy causing increased sensitivity to dietary Zn deficiency and reduced sensitivity to Cd toxicity. ..
  57. The Ubiquitin Proteasome Pathway & Synaptic Plasticity
    KELSEY MARTIN; Fiscal Year: 2005
    ..abstract_text> ..