golgi apparatus

Summary

Summary: A stack of flattened vesicles that functions in posttranslational processing and sorting of proteins, receiving them from the rough ENDOPLASMIC RETICULUM and directing them to secretory vesicles, LYSOSOMES, or the CELL MEMBRANE. The movement of proteins takes place by transfer vesicles that bud off from the rough endoplasmic reticulum or Golgi apparatus and fuse with the Golgi, lysosomes or cell membrane. (From Glick, Glossary of Biochemistry and Molecular Biology, 1990)

Top Publications

  1. ncbi Signals for sorting of transmembrane proteins to endosomes and lysosomes
    Juan S Bonifacino
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Annu Rev Biochem 72:395-447. 2003
  2. ncbi Phosphoinositides in cell regulation and membrane dynamics
    Gilbert Di Paolo
    Department of Pathology and Cell Biology, The Taub Institute for Research on Alzheimer s Disease and the Aging Brain, Columbia University Medical Center, New York, New York 10032, USA
    Nature 443:651-7. 2006
  3. ncbi ARF proteins: roles in membrane traffic and beyond
    CRISLYN D'SOUZA-SCHOREY
    Department of Biological Sciences and the Walther Cancer Institute, University of Notre Dame, Notre Dame, Indiana, USA
    Nat Rev Mol Cell Biol 7:347-58. 2006
  4. ncbi Functions of lipid rafts in biological membranes
    D A Brown
    Department of Biochemistry and Cell Biology, State University of New York at Stony Brook 11794 5215, USA
    Annu Rev Cell Dev Biol 14:111-36. 1998
  5. ncbi Intracellular functions of N-linked glycans
    A Helenius
    Institute of Biochemistry, , , , Switzerland
    Science 291:2364-9. 2001
  6. pmc A comprehensive comparison of transmembrane domains reveals organelle-specific properties
    Hayley J Sharpe
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, UK
    Cell 142:158-69. 2010
  7. ncbi Organelle-specific initiation of cell death pathways
    K F Ferri
    Centre National de la Recherche Scientifique, UMR 1599, Institut Gustave Roussy, Pavillon de Recherche 1, 39 rue Camille-Desmoulins, F-94805 Villejuif, France
    Nat Cell Biol 3:E255-63. 2001
  8. pmc NKG2D ligand MICA is retained in the cis-Golgi apparatus by human cytomegalovirus protein UL142
    Omodele Ashiru
    Department of Pathology, Cambridge Institute for Medical Research, University of Cambridge, Cambridge, United Kingdom
    J Virol 83:12345-54. 2009
  9. ncbi Systematic analysis of SNARE molecules in Arabidopsis: dissection of the post-Golgi network in plant cells
    Tomohiro Uemura
    Graduate School of Biostudies, Kyoto University, Yoshida nihonmatsu, Sakyo ku, Kyoto 606 8501, Japan
    Cell Struct Funct 29:49-65. 2004
  10. ncbi Ras proteins: different signals from different locations
    John F Hancock
    Institute for Molecular Bioscience and Department of Molecular and Cellular Pathology, University of Queensland, Brisbane, Australia 4072
    Nat Rev Mol Cell Biol 4:373-84. 2003

Detail Information

Publications266 found, 100 shown here

  1. ncbi Signals for sorting of transmembrane proteins to endosomes and lysosomes
    Juan S Bonifacino
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Annu Rev Biochem 72:395-447. 2003
    ..This complex array of signals and recognition proteins ensures the dynamic but accurate distribution of transmembrane proteins to different compartments of the endosomal-lysosomal system...
  2. ncbi Phosphoinositides in cell regulation and membrane dynamics
    Gilbert Di Paolo
    Department of Pathology and Cell Biology, The Taub Institute for Research on Alzheimer s Disease and the Aging Brain, Columbia University Medical Center, New York, New York 10032, USA
    Nature 443:651-7. 2006
    ..Their functions, besides classical signal transduction at the cell surface, include regulation of membrane traffic, the cytoskeleton, nuclear events and the permeability and transport functions of membranes...
  3. ncbi ARF proteins: roles in membrane traffic and beyond
    CRISLYN D'SOUZA-SCHOREY
    Department of Biological Sciences and the Walther Cancer Institute, University of Notre Dame, Notre Dame, Indiana, USA
    Nat Rev Mol Cell Biol 7:347-58. 2006
    ....
  4. ncbi Functions of lipid rafts in biological membranes
    D A Brown
    Department of Biochemistry and Cell Biology, State University of New York at Stony Brook 11794 5215, USA
    Annu Rev Cell Dev Biol 14:111-36. 1998
    ..Rafts may also function in sorting and trafficking through the secretory and endocytic pathways...
  5. ncbi Intracellular functions of N-linked glycans
    A Helenius
    Institute of Biochemistry, , , , Switzerland
    Science 291:2364-9. 2001
    ..The division of synthesis and processing between the ER and the Golgi complex represents an evolutionary adaptation that allows efficient exploitation of the potential of oligosaccharides...
  6. pmc A comprehensive comparison of transmembrane domains reveals organelle-specific properties
    Hayley J Sharpe
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, UK
    Cell 142:158-69. 2010
    ..The pervasive presence of organelle-specific features among the TMDs of a particular organelle has implications for TMD prediction, regulation of protein activity by location, and sorting of proteins and lipids in the secretory pathway...
  7. ncbi Organelle-specific initiation of cell death pathways
    K F Ferri
    Centre National de la Recherche Scientifique, UMR 1599, Institut Gustave Roussy, Pavillon de Recherche 1, 39 rue Camille-Desmoulins, F-94805 Villejuif, France
    Nat Cell Biol 3:E255-63. 2001
    ..evidence suggests that other organelles, including the endoplasmic reticulum (ER), lysosomes and the Golgi apparatus, are also major points of integration of pro-apoptotic signalling or damage sensing...
  8. pmc NKG2D ligand MICA is retained in the cis-Golgi apparatus by human cytomegalovirus protein UL142
    Omodele Ashiru
    Department of Pathology, Cambridge Institute for Medical Research, University of Cambridge, Cambridge, United Kingdom
    J Virol 83:12345-54. 2009
    ..Here, we show that UL142 localizes predominantly to the endoplasmic reticulum (ER) and cis-Golgi apparatus. The transmembrane domain of UL142 mediates its ER localization, while we propose that the UL142 luminal ..
  9. ncbi Systematic analysis of SNARE molecules in Arabidopsis: dissection of the post-Golgi network in plant cells
    Tomohiro Uemura
    Graduate School of Biostudies, Kyoto University, Yoshida nihonmatsu, Sakyo ku, Kyoto 606 8501, Japan
    Cell Struct Funct 29:49-65. 2004
    ..SNARE proteins were located on specific intracellular compartments: 6 in the endoplasmic reticulum, 9 in the Golgi apparatus, 4 in the trans-Golgi network (TGN), 2 in endosomes, 17 on the plasma membrane, 7 in both the prevacuolar ..
  10. ncbi Ras proteins: different signals from different locations
    John F Hancock
    Institute for Molecular Bioscience and Department of Molecular and Cellular Pathology, University of Queensland, Brisbane, Australia 4072
    Nat Rev Mol Cell Biol 4:373-84. 2003
    ..These different membrane microenvironments modulate Ras signal output and highlight the complex interplay between Ras location and function...
  11. pmc Role of oxysterol binding protein in hepatitis C virus infection
    Yutaka Amako
    Department of Medicine, University of California, San Diego, La Jolla, 92093, USA
    J Virol 83:9237-46. 2009
    ..The pleckstrin homology (PH) domain located in the N-terminal region of OSBP targeted this protein to the Golgi apparatus. OSBP deletion mutation in the PH (DeltaPH) domain failed to localize to the Golgi apparatus and inhibited ..
  12. pmc PtdIns4P recognition by Vps74/GOLPH3 links PtdIns 4-kinase signaling to retrograde Golgi trafficking
    Christopher S Wood
    Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, 19104, USA
    J Cell Biol 187:967-75. 2009
    Targeting and retention of resident integral membrane proteins of the Golgi apparatus underly the function of the Golgi in glycoprotein and glycolipid processing and sorting...
  13. pmc Alpha-synuclein blocks ER-Golgi traffic and Rab1 rescues neuron loss in Parkinson's models
    Antony A Cooper
    School of Biological Sciences, University of Missouri Kansas City, Kansas City, MO 64110, USA
    Science 313:324-8. 2006
    ..Thus, synucleinopathies may result from disruptions in basic cellular functions that interface with the unique biology of particular neurons to make them especially vulnerable...
  14. pmc Coatomer-dependent protein delivery to lipid droplets
    Krishnakant G Soni
    Cell Biology and Metabolism Program, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    J Cell Sci 122:1834-41. 2009
    ..Here we demonstrate that ATGL and ADRP, but not TIP47, are delivered to LDs by a pathway mediated by the COPI and COPII coatomer proteins and their corresponding regulators...
  15. pmc Exit from the Golgi is required for the expansion of the autophagosomal phagophore in yeast Saccharomyces cerevisiae
    Aniek van der Vaart
    Department of Cell Biology and Institute of Biomembranes, University Medical Center Utrecht, Utrecht 3584, The Netherlands
    Mol Biol Cell 21:2270-84. 2010
    ..Our data show that the Golgi complex plays a crucial role in supplying the lipid bilayers necessary for the biogenesis of double-membrane vesicles possibly through a new class of transport carriers or a new mechanism...
  16. pmc PTRF-Cavin, a conserved cytoplasmic protein required for caveola formation and function
    Michelle M Hill
    Institute for Molecular Bioscience, University of Queensland, Brisbane, Queensland 4072, Australia
    Cell 132:113-24. 2008
    ..We conclude that PTRF-Cavin is required for caveola formation and sequestration of mobile caveolin into immobile caveolae...
  17. pmc Membrane lipids: where they are and how they behave
    Gerrit van Meer
    Bijvoet Center and Institute of Biomembranes, Utrecht University, 3584 CH Utrecht, The Netherlands
    Nat Rev Mol Cell Biol 9:112-24. 2008
    ..enzymes, translocases and transporters, plus the intrinsic physical phase behaviour of lipids and their interactions with membrane proteins, to create the unique compositions and multiple functionalities of their individual membranes?..
  18. ncbi Ras signalling on the endoplasmic reticulum and the Golgi
    Vi K Chiu
    Department of Medicine, Cell Biology and Pharmacology, New York University School of Medicine, 550 First Avenue, New York, NY 10016, USA
    Nat Cell Biol 4:343-50. 2002
    ..Thus, Ras signalling is not limited to the PM, but also proceeds on the endomembrane...
  19. pmc GOLPH3 bridges phosphatidylinositol-4- phosphate and actomyosin to stretch and shape the Golgi to promote budding
    Holly C Dippold
    Division of Endocrinology and Metabolism, Department of Medicine, University of California, San Diego, La Jolla, CA 92093 0707, USA
    Cell 139:337-51. 2009
    ..Consequently, this tensile force stretches the Golgi into the extended ribbon observed by fluorescence microscopy and the familiar flattened form observed by electron microscopy...
  20. pmc The Parkinson's disease protein alpha-synuclein disrupts cellular Rab homeostasis
    Aaron D Gitler
    Whitehead Institute for Biomedical Research and Howard Hughes Medical Institute, Cambridge, MA 02142, USA
    Proc Natl Acad Sci U S A 105:145-50. 2008
    ..Thus, alpha-syn causes general defects in vesicle trafficking, to which dopaminergic neurons are especially sensitive...
  21. doi TGN golgins, Rabs and cytoskeleton: regulating the Golgi trafficking highways
    Bruno Goud
    Institut Curie, CNRS UMR144, 26, rue d Ulm, Paris cedex 75248, France
    Trends Cell Biol 20:329-36. 2010
    The architecture of the Golgi apparatus is intimately linked to its role in regulating membrane trafficking...
  22. ncbi The human phosphatidylinositol phosphatase SAC1 interacts with the coatomer I complex
    Holger M Rohde
    Boehringer Ingelheim Austria GmbH, Dr Boehringer Gasse 5 11, 1121 Vienna, Austria
    J Biol Chem 278:52689-99. 2003
    ..cerevisiae SAC1 gene encodes an integral membrane protein of the endoplasmic reticulum (ER) and the Golgi apparatus. Yeast SAC1 mutants display a wide array of phenotypes including inositol auxotrophy, cold sensitivity, ..
  23. doi The palmitoylation machinery is a spatially organizing system for peripheral membrane proteins
    Oliver Rocks
    European Molecular Biology Laboratory, Meyerhofstrasse 1, 69118 Heidelberg, Germany
    Cell 141:458-71. 2010
    ..This generic spatially organizing system differs from conventional receptor-mediated targeting mechanisms and efficiently counteracts entropy-driven redistribution of palmitoylated peripheral membrane proteins over all membranes...
  24. doi Structural organization of the Golgi apparatus
    Martin Lowe
    Faculty of Life Sciences, University of Manchester, The Michael Smith Building, Oxford Road, Manchester M13 9PT, UK
    Curr Opin Cell Biol 23:85-93. 2011
    The Golgi apparatus is a universal feature of eukaryotes, carrying out the key functions of processing, sorting and trafficking of newly synthesized membrane and secretory proteins...
  25. pmc Secreted kinase phosphorylates extracellular proteins that regulate biomineralization
    Vincent S Tagliabracci
    Department of Pharmacology, University of California, San Diego, La Jolla, CA 92093 0721, USA
    Science 336:1150-3. 2012
    ..We identified a family of atypical protein kinases that localize within the Golgi apparatus and are secreted...
  26. doi Multiple cytosolic and transmembrane determinants are required for the trafficking of SCAMP1 via an ER-Golgi-TGN-PM pathway
    Yi Cai
    School of Life Sciences, Centre for Cell and Developmental Biology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China
    Plant J 65:882-96. 2011
    ..Therefore, both the cytosolic N-terminus and TMD sequences of SCAMP1 play integral roles in mediating its transport to the PM via an ER-Golgi-TGN pathway...
  27. ncbi Mutations in dynein link motor neuron degeneration to defects in retrograde transport
    Majid Hafezparast
    Department of Neurodegenerative Disease, Institute of Neurology, National Hospital for Neurology and Neurosurgery, Queen Square, London WC1N 3BG, UK
    Science 300:808-12. 2003
    ..These mutations exclusively perturb neuron-specific functions of dynein...
  28. doi Exiting the Golgi complex
    Maria Antonietta De Matteis
    Department of Cell Biology and Oncology, Consorzio Mario Negri Sud, 66030 Santa Maria Imbaro CH, Italy
    Nat Rev Mol Cell Biol 9:273-84. 2008
    ....
  29. pmc Loss of Apm1, the micro1 subunit of the clathrin-associated adaptor-protein-1 complex, causes distinct phenotypes and synthetic lethality with calcineurin deletion in fission yeast
    Ayako Kita
    Division of Molecular Pharmacology and Pharmacogenomics, Department of Genome Sciences, Kobe University Graduate School of Medicine, Kobe 650 0017, Japan
    Mol Biol Cell 15:2920-31. 2004
    ....
  30. pmc Coordinated lipid transfer between the endoplasmic reticulum and the Golgi complex requires the VAP proteins and is essential for Golgi-mediated transport
    Diego Peretti
    The Molecular Cell Biology Department, Weizmann Institute of Science, Rehovot 76100, Israel
    Mol Biol Cell 19:3871-84. 2008
    ..We propose that Nir2, OSBP, and CERT function coordinately at the ER-Golgi membrane contact sites, thereby affecting the lipid composition of the Golgi membranes and consequently their structural and functional identities...
  31. pmc A comparative study of the involvement of 17 Arabidopsis myosin family members on the motility of Golgi and other organelles
    Dror Avisar
    Institute of Plant Sciences, Volcani Center, Bet Dagan 50250, Israel
    Plant Physiol 150:700-9. 2009
    ..Taken together, our data suggest that the above myosins are involved, directly or indirectly, in the movement of Golgi and mitochondria in plant cells...
  32. pmc Arf6 and microtubules in adhesion-dependent trafficking of lipid rafts
    Nagaraj Balasubramanian
    Robert M Beirne Cardiovascular Research Center, Mellon Prostate Cancer Research Institute, University of Virginia, Charlottesville, Virginia 22908, USA
    Nat Cell Biol 9:1381-91. 2007
    ..Both of these steps are required for the plasma membrane targeting of Rac1 and for its activation. These data therefore define a new membrane raft trafficking pathway that is crucial for anchorage-dependent signalling...
  33. doi Functional interplay between LIS1, NDE1 and NDEL1 in dynein-dependent organelle positioning
    Connie Lam
    Faculty of Life Sciences, University of Manchester, Manchester, M13 9PT, UK
    J Cell Sci 123:202-12. 2010
    ..Consistent with this hypothesis, expression of exogenous NDE1 or NDEL1 rescued the effects of LIS1 depletion on Golgi organisation, whereas LIS1 was only partially effective at rescuing the loss of NDE1 and NDEL1...
  34. pmc Nanoscale architecture of endoplasmic reticulum export sites and of Golgi membranes as determined by electron tomography
    L Andrew Staehelin
    Molecular Cellular and Developmental Biology, University of Colorado, Boulder, Colorado 80309, USA
    Plant Physiol 147:1454-68. 2008
  35. ncbi A Golgi-associated PDZ domain protein modulates cystic fibrosis transmembrane regulator plasma membrane expression
    Jie Cheng
    Department of Physiology and Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Biol Chem 277:3520-9. 2002
    ..CAL is located primarily at the Golgi apparatus, co-localizing with trans-Golgi markers and is sensitive to Brefeldin A treatment...
  36. pmc Recycling of golgi-resident glycosyltransferases through the ER reveals a novel pathway and provides an explanation for nocodazole-induced Golgi scattering
    B Storrie
    Department of Biochemistry, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061 0308, USA
    J Cell Biol 143:1505-21. 1998
    During microtubule depolymerization, the central, juxtanuclear Golgi apparatus scatters to multiple peripheral sites...
  37. ncbi Golgi recruitment of GRIP domain proteins by Arf-like GTPase 1 is regulated by Arf-like GTPase 3
    Subba Rao Gangi Setty
    University of Pennsylvania School of Medicine, Department of Cell and Developmental Biology, 421 Curie Boulevard, BRB 2 3, Room 1010, Philadelphia, PA 19104 6058, USA
    Curr Biol 13:401-4. 2003
    ..The similar requirements for localization of GRIP domains from yeast, mouse, and human when expressed in yeast, and the presence of Arl1p and Arl3p homologs in these species, suggest that this is an evolutionarily conserved mechanism...
  38. doi Deletion mutants of AP-1 adaptin subunits display distinct phenotypes in fission yeast
    Yan Ma
    Division of Molecular Pharmacology and Pharmacogenomics, Department of Biochemistry and Molecular Biology, Kobe University Graduate School of Medicine, Kusunoki cho, Chuo Ku, Kobe 650 0017, Japan
    Genes Cells 14:1015-28. 2009
    ..Altogether, results suggest that the four subunits are all essential for the heterotetrameric complex formation and for the AP-1 function in exit transport from endosomes...
  39. pmc Assembly and maturation of the flavivirus Kunjin virus appear to occur in the rough endoplasmic reticulum and along the secretory pathway, respectively
    J M MacKenzie
    Clinical Medical Virology Centre, University of Queensland, St Lucia, Queensland 4072, Australia
    J Virol 75:10787-99. 2001
    ..virions from the RER involves the transport of individual virions within individual vesicles en route to the Golgi apparatus. The results suggest that the assembly of virions occurs within the lumen of the RER and that subsequent ..
  40. pmc Is the 6 kDa tobacco etch viral protein a bona fide ERES marker?
    Alexander Lerich
    Department of Plant Cell Biology, Centre for Organismal Studies, University of Heidelberg, Germany
    J Exp Bot 62:5013-23. 2011
    ..However, based on a consideration of the structure of its cytoplasmic tail, the scenario that the VP collects at ERES and is transported to the cis-Golgi before being recycled back to the ER, is supported...
  41. doi Rab and actomyosin-dependent fission of transport vesicles at the Golgi complex
    S Miserey-Lenkei
    Institut Curie, CNRS UMR144, Molecular mechanisms of intracellular transport Lab, 26 Rue d Ulm, 75248 Paris Cedex 05, France
    Nat Cell Biol 12:645-54. 2010
    ..Our results provide evidence that the actomyosin system is required in vesicle biogenesis at the Golgi, and uncover a function for Rab GTPases in vesicle fission...
  42. pmc Phosphoinositide 3-kinase δ regulates membrane fission of Golgi carriers for selective cytokine secretion
    Pei Ching Low
    Institute for Molecular Bioscience, University of Queensland, Brisbane, Queensland 4072, Australia
    J Cell Biol 190:1053-65. 2010
    ..These findings demonstrate a new function for p110δ as part of the membrane fission machinery required at the TGN for the selective trafficking and secretion of cytokines in macrophages...
  43. pmc Arfaptins are localized to the trans-Golgi by interaction with Arl1, but not Arfs
    Zhiqiu Man
    Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto, Japan
    J Biol Chem 286:11569-78. 2011
    ..These observations indicate that arfaptins are recruited onto trans-Golgi membranes by interacting with Arl1, and capable of inducing membrane deformation via their BAR domains...
  44. doi Signalling to and from the secretory pathway
    Hesso Farhan
    Biozentrum, University of Basel, Klingelbergstrasse 50 70, Basel, Switzerland
    J Cell Sci 124:171-80. 2011
    ..This Commentary highlights recent contributions to our understanding of the mutual regulation of the secretory pathway and cellular signalling...
  45. pmc The destination for single-pass membrane proteins is influenced markedly by the length of the hydrophobic domain
    Federica Brandizzi
    Research School of Biological and Molecular Sciences, Oxford Brookes University, Gipsy Lane Campus, OX3 0BP Headington Oxford, United Kingdom
    Plant Cell 14:1077-92. 2002
    ..of the TM20-labeled compartments was investigated by coexpression with a marker localized mainly in the Golgi apparatus, AtERD2, fused to a yellow fluorescent protein...
  46. doi The N-terminal pro region mediates retention of unprocessed type-I PME in the Golgi apparatus
    Sebastian Wolf
    Heidelberg Institute for Plant Sciences, INF 360, 69120 Heidelberg, Germany
    Plant J 58:361-75. 2009
    ..indicate that the pro region operates as an effective retention mechanism, keeping unprocessed PME in the Golgi apparatus. Consequently, pro-protein processing could constitute a post-translational mechanism regulating PME activity.
  47. pmc Dissection of Arabidopsis ADP-RIBOSYLATION FACTOR 1 function in epidermal cell polarity
    Jian Xu
    Department of Molecular Cell Biology, Utrecht University, 3584 CH Utrecht, The Netherlands
    Plant Cell 17:525-36. 2005
    ..Fluorescence colocalization studies reveal that ARF1 is localized to the Golgi apparatus and endocytic organelles in both onion (Allium cepa) and Arabidopsis cells...
  48. pmc Impact on the endoplasmic reticulum and Golgi apparatus of turnip mosaic virus infection
    Romain Grangeon
    INRS Institut Armand Frappier, Institut National de la Recherche Scientifique, Laval, Quebec, Canada
    J Virol 86:9255-65. 2012
    ..TuMV infection led to the amalgamation of the endoplasmic reticulum (ER), Golgi apparatus, COPII coatamers, and chloroplasts into a perinuclear globular structure that also contained viral proteins...
  49. pmc A missense mutation in the Arabidopsis COPII coat protein Sec24A induces the formation of clusters of the endoplasmic reticulum and Golgi apparatus
    Carmen Faso
    Department of Energy, Plant Research Laboratory, Michigan State University, East Lansing, MI 48824, USA
    Plant Cell 21:3655-71. 2009
    How the endoplasmic reticulum (ER) and the Golgi apparatus maintain their morphological and functional identity while working in concert to ensure the production of biomolecules necessary for the cell's survival is a fundamental question ..
  50. pmc Regulation of vascular endothelial growth factor receptor 2 trafficking and angiogenesis by Golgi localized t-SNARE syntaxin 6
    Venkatraman Manickam
    Department of Anatomy and Cell Biology, University of Iowa, 51 Newton Rd, Iowa City, IA 52242, USA
    Blood 117:1425-35. 2011
    ..Plasma membrane (PM) levels of VEGFR2 are regulated by endocytosis and secretory transport through the Golgi apparatus. To date, the mechanism whereby the VEGFR2 traffics through the Golgi apparatus remains incompletely ..
  51. ncbi Distinct AAA-ATPase p97 complexes function in discrete steps of nuclear assembly
    M Hetzer
    European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    Nat Cell Biol 3:1086-91. 2001
    ..Subsequent NE growth involves a p97-p47 complex. This study provides the first insights into the molecular mechanisms and specificity of fusion events involved in NE formation...
  52. ncbi Protein sorting to the storage vacuoles of plants: a critical appraisal
    David G Robinson
    Heidelberg Institute for Plant Sciences, Cell Biology, University of Heidelberg, Im Neuenheimer Feld 230, D 69120 Heidelberg, Germany
    Traffic 6:615-25. 2005
    ..As a consequence, the plant Golgi apparatus is faced with the problem of recognizing proteins destined for lytic and storage vacuoles and segregating ..
  53. doi A myosin XI tail domain homologous to the yeast myosin vacuole-binding domain interacts with plastids and stromules in Nicotiana benthamiana
    Amir Sattarzadeh
    Department of Molecular Biology and Genetics, Biotechnology Building, Cornell University, Ithaca, NY 14853, USA
    Mol Plant 2:1351-8. 2009
    ..benthamiana. Taken together, these observations implicate myosin motor proteins in dynamics of plastids and stromules...
  54. pmc Salt tolerance of Arabidopsis thaliana requires maturation of N-glycosylated proteins in the Golgi apparatus
    Jae Sook Kang
    Brain Korea 21, Division of Applied Life Science and Environmental Biotechnology National Core Research Center, Graduate School of Gyeongsang National University, Jinju 660 701, Korea
    Proc Natl Acad Sci U S A 105:5933-8. 2008
    Protein N-glycosylation in the endoplasmic reticulum (ER) and in the Golgi apparatus is an essential process in eukaryotic cells...
  55. pmc Identification of a xylogalacturonan xylosyltransferase involved in pectin biosynthesis in Arabidopsis
    Jacob Krüger Jensen
    Laboratory of Molecular Plant Biology, Department of Plant Biology, Faculty of Life Sciences, University of Copenhagen, DK 1871 Frederiksberg C, Denmark
    Plant Cell 20:1289-302. 2008
    ..The protein was shown by expression of a fluorescent fusion protein in N. benthamiana to be localized in the Golgi vesicles as expected for a glycosyltransferase involved in pectin biosynthesis...
  56. pmc The rice alpha-amylase glycoprotein is targeted from the Golgi apparatus through the secretory pathway to the plastids
    Aya Kitajima
    Graduate School of Science and Technology, Niigata University, Niigata, Japan
    Plant Cell 21:2844-58. 2009
    ..Thus, Golgi-to-plastid traffic appears to be involved in the transport of glycoproteins to plastids and plastid targeting seems to be accomplished in a sorting signal-dependent manner...
  57. pmc Sterol-regulated transport of SREBPs from endoplasmic reticulum to Golgi: oxysterols block transport by binding to Insig
    Arun Radhakrishnan
    Department of Molecular Genetics and Biochemistry, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    Proc Natl Acad Sci U S A 104:6511-8. 2007
    ..These studies define Insigs as oxysterol-binding proteins, explaining the long-known ability of oxysterols to inhibit cholesterol synthesis in animal cells...
  58. pmc Rabs and their effectors: achieving specificity in membrane traffic
    Bianka L Grosshans
    Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06520, USA
    Proc Natl Acad Sci U S A 103:11821-7. 2006
    ..Additionally, Rab cascades and Rab conversions appear to confer directionality to membrane traffic and couple each stage of traffic with the next along the pathway...
  59. ncbi Secretory COPII coat component Sec23a is essential for craniofacial chondrocyte maturation
    Michael R Lang
    Department of Medicine, Division of Genetic Medicine, Vanderbilt University Medical Center, Nashville, Tennessee 37232, USA
    Nat Genet 38:1198-203. 2006
    ..of the COPII complex, which is critical for anterograde protein trafficking between endoplasmic reticulum and Golgi apparatus. Zebrafish crusher mutants develop normally until the onset of craniofacial chondrogenesis...
  60. ncbi Interaction of tyrosine-based sorting signals with clathrin-associated proteins
    H Ohno
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Science 269:1872-5. 1995
    ..The interaction was confirmed by in vitro binding assays. Thus, it is likely that the medium chains serve as signal-binding components of the clathrin-dependent sorting machinery...
  61. ncbi Bi-directional protein transport between the ER and Golgi
    Marcus C S Lee
    Howard Hughes Medical Institute and Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA
    Annu Rev Cell Dev Biol 20:87-123. 2004
    ..We review the mechanisms that govern coat recruitment to the membrane, cargo capture into a transport vesicle, and accurate delivery to the target organelle...
  62. ncbi The ZIP7 gene (Slc39a7) encodes a zinc transporter involved in zinc homeostasis of the Golgi apparatus
    Liping Huang
    Agricultural Research Service, United States Department of Agriculture, Western Human Nutrition Research Center, University of California at Davis, 95616, USA
    J Biol Chem 280:15456-63. 2005
    ..The endogenous ZIP7 was associated with the Golgi apparatus and was capable of transporting zinc from the Golgi apparatus into the cytoplasm of the cell...
  63. doi Tracing the retrograde route in protein trafficking
    Ludger Johannes
    CNRS UMR144, Centre de Recherche, Traffic, Signaling, and Delivery Laboratory, 75248 Paris Cedex 05, France
    Cell 135:1175-87. 2008
    ..which proteins and lipids are shuttled between endosomes and biosynthetic/secretory compartments such as the Golgi apparatus, is crucial for a diverse range of cellular functions...
  64. pmc Asymmetric CLASP-dependent nucleation of noncentrosomal microtubules at the trans-Golgi network
    Andrey Efimov
    Department of Cell and Developmental Biology, Vanderbilt University Medical Center, Nashville, TN 37232, USA
    Dev Cell 12:917-30. 2007
    ..Here we demonstrate that a large number of microtubules in untreated human cells originate from the Golgi apparatus in a centrosome-independent manner...
  65. doi Chlamydia causes fragmentation of the Golgi compartment to ensure reproduction
    Dagmar Heuer
    Department of Molecular Biology, Max Planck Institute for Infection Biology, Chariteplatz 1, 10117 Berlin, Germany
    Nature 457:731-5. 2009
    ..for host cell lipids, such as sphingolipids and cholesterol, produced in the endoplasmic reticulum and the Golgi apparatus. However, the mechanisms by which intracellular pathogens acquire host cell lipids are not well understood...
  66. pmc Identification of a family of animal sphingomyelin synthases
    Klazien Huitema
    Department of Membrane Enzymology, Faculty of Chemistry, Institute of Biomembranes, Utrecht University, Utrecht, The Netherlands
    EMBO J 23:33-44. 2004
    ..Whereas human SMS1 is localised to the Golgi, SMS2 resides primarily at the plasma membrane. Collectively, these findings open up important new avenues for studying sphingolipid function in animals...
  67. pmc Class 1 reversibly glycosylated polypeptides are plasmodesmal-associated proteins delivered to plasmodesmata via the golgi apparatus
    Guy Sagi
    Department of Plant Sciences, George S Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel
    Plant Cell 17:1788-800. 2005
    ..Localization to the Golgi apparatus was verified by colocalization of transiently expressed AtRGP2 fused to cyan fluorescence protein together ..
  68. pmc GATE-16, a membrane transport modulator, interacts with NSF and the Golgi v-SNARE GOS-28
    Y Sagiv
    Department of Biological Chemistry, The Weizmann Institute of Science, Rehovot, 76100 Israel
    EMBO J 19:1494-504. 2000
    ..It also interacts with the Golgi v-SNARE GOS-28 in an NSF-dependent manner. We propose that GATE-16 modulates intra-Golgi transport through coupling between NSF activity and SNAREs activation...
  69. pmc Atg27 is required for autophagy-dependent cycling of Atg9
    Wei Lien Yen
    Life Sciences Institute, and Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA
    Mol Biol Cell 18:581-93. 2007
    ..These data support a hypothesis that multiple membrane sources supply the lipids needed for autophagosome formation...
  70. pmc UNC-108/RAB-2 and its effector RIC-19 are involved in dense core vesicle maturation in Caenorhabditis elegans
    Marija Sumakovic
    European Neuroscience Institute Goettingen, German Research Foundation Research Center for Molecular Physiology of the Brain, 37077 Goettingen, Germany
    J Cell Biol 186:897-914. 2009
    ..Thus, we propose that RAB-2 and its effector RIC-19 are required for neuronal DCV maturation...
  71. doi Subcompartment localization of the side chain xyloglucan-synthesizing enzymes within Golgi stacks of tobacco suspension-cultured cells
    Laurence Chevalier
    Laboratoire Glycobiologie et Matrice Extracellulaire Végétale, UPRES EA 4358, Institut Federatif de Recherche Multidisciplinaire sur les Peptides 23, Plate forme de Recherche en Imagerie Cellulaire de Haute Normandie PRIMACEN, IBiSA, Universite de Rouen, 76821 Mont Saint Aignan Cedex, France
    Plant J 64:977-89. 2010
    ..that: (i) all fusion proteins, AtXT1-GFP, AtMUR3-GFP and AtFUT1-GFP are specifically targeted to the Golgi apparatus; and (ii) AtXT1-GFP is mainly located in the cis and medial cisternae, AtMUR3-GFP is predominantly associated ..
  72. pmc The GTPase ARF1p controls the sequence-specific vacuolar sorting route to the lytic vacuole
    Peter Pimpl
    Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, United Kingdom
    Plant Cell 15:1242-56. 2003
    ..We have studied the transport of soluble cargo molecules by inhibiting specific transport steps to and from the Golgi apparatus. Inhibition of export from the Golgi via coexpression of a dominant-negative GTP-restricted ARF1 mutant (Q71L)..
  73. doi The syntaxins SYP31 and SYP81 control ER-Golgi trafficking in the plant secretory pathway
    Julia Bubeck
    Department of Cell Biology, Heidelberg Institute for Plant Sciences, University of Heidelberg, Heidelberg, Germany
    Traffic 9:1629-52. 2008
    ..These punctate structures were physically separated from the Golgi, suggesting that they might possibly reflect the position of ER import sites...
  74. pmc Golgi-derived CLASP-dependent microtubules control Golgi organization and polarized trafficking in motile cells
    Paul M Miller
    Department of Cell and Developmental Biology, Vanderbilt University Medical Center, Nashville, TN 37232, USA
    Nat Cell Biol 11:1069-80. 2009
    ....
  75. doi BFA-induced compartments from the Golgi apparatus and trans-Golgi network/early endosome are distinct in plant cells
    Sheung Kwan Lam
    Department of Biology and Molecular Biotechnology Program, Centre for Cell and Developmental Biology, State China Key Laboratory for Agrobiotechnology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China
    Plant J 60:865-81. 2009
    ..At low concentrations (5-10 microg ml(-1)), BFA caused both the Golgi apparatus and trans-Golgi network (TGN), an early endosome (EE) equivalent in plant cells, to form visible aggregates ..
  76. ncbi Separate roles for the Golgi apparatus and lysosomes in the sequestration of drugs in the multidrug-resistant human leukemic cell line HL-60
    Yuping Gong
    Division of Drug Delivery and Disposition, School of Pharmacy, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    J Biol Chem 278:50234-9. 2003
    ..pH partitioning type mechanism, whereas sulforhodamime 101, a zwitterionic molecule, is sequestered into the Golgi apparatus through a drug transporter-mediated process...
  77. ncbi Role of Erv29p in collecting soluble secretory proteins into ER-derived transport vesicles
    W J Belden
    Department of Biochemistry, Dartmouth Medical School, Hanover, NH 03755, USA
    Science 294:1528-31. 2001
    ..In vivo, export of gpalphaf from the ER was saturable and depended on the expression level of Erv29p. These results indicate that membrane receptors can link soluble cargo proteins to the COPII coat...
  78. ncbi Adult-onset primary open-angle glaucoma caused by mutations in optineurin
    Tayebeh Rezaie
    Molecular Ophthalmic Genetics Laboratory, Surgical Research Center, Department of Surgery, University of Connecticut Health Center, Farmington, CT 06030, USA
    Science 295:1077-9. 2002
    ..Optineurin is expressed in trabecular meshwork, nonpigmented ciliary epithelium, retina, and brain, and we speculate that it plays a neuroprotective role...
  79. doi In vivo trafficking and localization of p24 proteins in plant cells
    Markus Langhans
    Department of Cell Biology, Heidelberg Institute for Plant Sciences, University of Heidelberg, 69120 Heidelberg, Germany
    Traffic 9:770-85. 2008
    ..Finally, we have found that ER export of Atp24 is COPII dependent, while its ER localization requires COPI function, presumably for efficient Golgi to ER recycling...
  80. ncbi Molecular cloning and characterization of Arabidopsis thaliana Golgi alpha-mannosidase II, a key enzyme in the formation of complex N-glycans in plants
    Richard Strasser
    Institute of Applied Genetics and Cell Biology, Vienna, Austria
    Plant J 45:789-803. 2006
    ..domain and 10 amino acids from the cytoplasmic tail are sufficient to retain a reporter molecule in the Golgi apparatus and that lumenal sequences are not involved in the retention mechanism...
  81. pmc Reevaluation of the effects of brefeldin A on plant cells using tobacco Bright Yellow 2 cells expressing Golgi-targeted green fluorescent protein and COPI antisera
    Christophe Ritzenthaler
    Institut de Biologie Moleculaire des Plantes, 67084 Strasbourg Cedex, France
    Plant Cell 14:237-61. 2002
    ..A (BFA) causes a block in the secretory system of eukaryotic cells by inhibiting vesicle formation at the Golgi apparatus. Although this toxin has been used in many studies, its effects on plant cells are still shrouded in ..
  82. pmc Movement and remodeling of the endoplasmic reticulum in nondividing cells of tobacco leaves
    I Sparkes
    School of Life Sciences, Oxford Brookes University, Oxford OX3 0BP, United Kingdom
    Plant Cell 21:3937-49. 2009
    ....
  83. doi Golgi transporters: opening the gate to cell wall polysaccharide biosynthesis
    Francisca Reyes
    The Millennium Nucleus in Plant Cell Biotechnology, Fundación Ciencia para la Vida, Center of Plant Biotechnology, Andres Bello University, Republica 217, Santiago, Chile
    Curr Opin Plant Biol 11:244-51. 2008
    The synthesis of non-cellulosic polysaccharides occurs in the Golgi apparatus and requires a great number of metabolites (substrates and ions)...
  84. pmc Nitric oxide synthase generates nitric oxide locally to regulate compartmentalized protein S-nitrosylation and protein trafficking
    Yasuko Iwakiri
    Section of Digestive Diseases, Department of Pharmacology, Yale University School of Medicine, New Haven, CT 06510, USA
    Proc Natl Acad Sci U S A 103:19777-82. 2006
    ..Endothelial NOS (eNOS) is localized mainly on the Golgi apparatus and in plasma membrane caveolae...
  85. pmc Alpha-synuclein-induced aggregation of cytoplasmic vesicles in Saccharomyces cerevisiae
    James H Soper
    Center for Neurodegenerative Disease Research and Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
    Mol Biol Cell 19:1093-103. 2008
    ....
  86. pmc MAPK signaling to the early secretory pathway revealed by kinase/phosphatase functional screening
    Hesso Farhan
    Biozentrum, Universitat Basel, 4056 Basel, Switzerland
    J Cell Biol 189:997-1011. 2010
    ..122 kinases/phosphatases that affect endoplasmic reticulum (ER) export, ER exit sites (ERESs), and/or the Golgi apparatus. Numerous kinases/phosphatases regulate the number of ERESs and ER to Golgi protein trafficking...
  87. ncbi The Golgi apparatus is a primary site of intracellular damage after photosensitization with Rose Bengal acetate
    C Soldani
    Dipartimento di Biologia Animale, Universita degli Studi di Pavia, Piazza Botta 10, 27100 Pavia, Italy
    Eur J Histochem 48:443-8. 2004
    The aim of the present investigation was to elucidate whether the Golgi apparatus undergoes photodamage following administration of the fluorogenic substrates Rose Bengal acetate (RBAc) and irradiation at the appropriate wavelength...
  88. doi Golgi apparatus fragmentation as a mechanism responsible for uniform delivery of uroplakins to the apical plasma membrane of uroepithelial cells
    Mateja Erdani Kreft
    Institute of Cell Biology, Faculty of Medicine, University of Ljubljana, SI 1000 Ljubljana, Slovenia
    Biol Cell 102:593-607. 2010
    The GA (Golgi apparatus) has an essential role in membrane trafficking, determining the assembly and delivery of UPs (uroplakins) to the APM (apical plasma membrane) of superficial UCs (uroepithelial cells) of urinary bladder...
  89. pmc Lipids and cholesterol as regulators of traffic in the endomembrane system
    Jennifer Lippincott-Schwartz
    Cell Biology and Metabolism Program, NICHD, NIH, Bethesda, Maryland, USA
    Annu Rev Biophys 39:559-78. 2010
    ..pathways-biosynthetic and endocytic, each with specific lipid components-make up this system, with the Golgi apparatus mediating transport between the two...
  90. pmc Arabidopsis thaliana RGXT1 and RGXT2 encode Golgi-localized (1,3)-alpha-D-xylosyltransferases involved in the synthesis of pectic rhamnogalacturonan-II
    Jack Egelund
    Biotechnology Group, Danish Institute of Agricultural Sciences and Center for Molecular Plant Physiology, DK 1871 Frederiksberg C, Denmark
    Plant Cell 18:2593-607. 2006
    ..and also colocalized with the Golgi marker dye BODIPY TR ceramide, consistent with targeting to the Golgi apparatus. Taken together, these results suggest that RGXT1 and RGXT2 encode Golgi-localized (1,3)-alpha-d-..
  91. pmc Association of the cellular coactivator HCF-1 with the Golgi apparatus in sensory neurons
    Gaelle Kolb
    National Institutes of Health, 4 129, 4 Center Drive, Bethesda, MD 20892, USA
    J Virol 82:9555-63. 2008
    ..Using a primary neuronal cell culture system, HCF-1 was localized to the Golgi apparatus in unstimulated neurons, a unique location for a transcriptional coactivator...
  92. pmc A genome-wide RNA interference screen identifies two novel components of the metazoan secretory pathway
    Franz Wendler
    MRC National Institute for Medical Research, London, UK
    EMBO J 29:304-14. 2010
    ..Given this coverage, the small number of novel components suggests that contrary to previous indications the number of essential core components of the secretory pathway is not much greater in metazoans than in yeasts...
  93. pmc Sequential interactions with Sec23 control the direction of vesicle traffic
    Christopher Lord
    Department of Cellular and Molecular Medicine, Howard Hughes Medical Institute, University of California at San Diego, La Jolla, California 92093 0668, USA
    Nature 473:181-6. 2011
    ..These events are conserved in mammalian cells...
  94. pmc SAP97 and CASK mediate sorting of NMDA receptors through a previously unknown secretory pathway
    Okunola Jeyifous
    Department of Psychiatry and Behavioral Science, Stanford University, Palo Alto, California, USA
    Nat Neurosci 12:1011-9. 2009
    ..These findings indicate that NMDARs are sorted away from AMPARs via a non-conventional secretory pathway that utilizes dendritic Golgi outposts...
  95. doi Hypo-phosphorylation of salivary peptidome as a clue to the molecular pathogenesis of autism spectrum disorders
    Massimo Castagnola
    Istituto di Biochimica e Biochimica Clinica, Facolta di Medicina, Universita Cattolica, Rome, Italy
    J Proteome Res 7:5327-32. 2008
    ..These results suggest that analysis of salivary phospho-peptides might help to discriminate a considerable subgroup of ASD patients...
  96. doi Loss of the Batten disease gene CLN3 prevents exit from the TGN of the mannose 6-phosphate receptor
    Daniel J Metcalf
    MRC Cell Biology Unit, MRC Laboratory for Molecular Cell Biology and Department of Cell and Developmental Biology, University College London, Gower Street, London WC1E6BT, UK
    Traffic 9:1905-14. 2008
    ..CLN3 is therefore essential for trafficking along the route needed for delivery of lysosomal enzymes, and its loss thereby contributes to and may explain the lysosomal dysfunction underlying Batten disease...
  97. pmc Regulation of dynactin through the differential expression of p150Glued isoforms
    Ram Dixit
    Department of Physiology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
    J Biol Chem 283:33611-9. 2008
    ..Together, these studies provide further insight into the regulation of dynein-dynactin function in the cell...
  98. pmc How the Golgi works: a cisternal progenitor model
    Suzanne R Pfeffer
    Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305 5307, USA
    Proc Natl Acad Sci U S A 107:19614-8. 2010
    ....
  99. pmc Direct binding of ubiquitin conjugates by the mammalian p97 adaptor complexes, p47 and Ufd1-Npl4
    Hemmo H Meyer
    Department of Cell Biology, Yale University School of Medicine, 333 Cedar Street, SHM, C441, PO Box 208002, New Haven, CT 06520 8002, USA
    EMBO J 21:5645-52. 2002
    ..In the case of p47, which is involved in the reassembly of the ER, the nuclear envelope and the Golgi apparatus, binding is mediated by a UBA domain...
  100. pmc Death receptor ligation triggers membrane scrambling between Golgi and mitochondria
    S Ouasti
    Faculty of Life Sciences, The University of Manchester, Stopford Building, Oxford Road, Manchester, UK
    Cell Death Differ 14:453-61. 2007
    ..Thus, our work unveils that Fas ligand-mediated apoptosis induces scrambling of mitochondrial and secretory organelles via a global alteration of membrane traffic that is modulated by apical caspases...
  101. pmc The hydrophobic domain of infectious bronchitis virus E protein alters the host secretory pathway and is important for release of infectious virus
    Travis R Ruch
    Department of Cell Biology, The Johns Hopkins University School of Medicine, 725 N Wolfe St, Baltimore, MD 21205, USA
    J Virol 85:675-85. 2011
    ..Our results indicated that the hydrophobic domain of IBV E alters the host secretory pathway to the apparent advantage of the virus...

Research Grants82

  1. Distance-Dependent Structure and Function of Neuronal Dendrites
    Kristen Harris; Fiscal Year: 2007
    ..that host excitory synapses, including microtubules for transport, polyribosomes for protein synthesis, Golgi apparatus for posttranslational modifications, endosomes for membrane recycling, and smooth endoplasmic reticulum (SER) ..
  2. Distance-Dependent Structure and Function of Neuronal Dendrites
    Kristen Harris; Fiscal Year: 2009
    ..that host excitory synapses, including microtubules for transport, polyribosomes for protein synthesis, Golgi apparatus for posttranslational modifications, endosomes for membrane recycling, and smooth endoplasmic reticulum (SER) ..
  3. Distance-Dependent Structure and Function of Neuronal Dendrites
    Kristen M Harris; Fiscal Year: 2010
    ..that host excitory synapses, including microtubules for transport, polyribosomes for protein synthesis, Golgi apparatus for posttranslational modifications, endosomes for membrane recycling, and smooth endoplasmic reticulum (SER) ..
  4. Membrane Protein Trafficking in Photoreceptors
    Wolfgang Baehr; Fiscal Year: 2010
    ..Integral membrane proteins are synthesized by ER-associated ribosomes and exported to the Golgi apparatus. Prenylated or acylated peripheral membrane proteins are synthesized in the cytosol and become ER-associated...
  5. Membrane Protein Trafficking in Photoreceptors
    Wolfgang Baehr; Fiscal Year: 2011
    ..Integral membrane proteins are synthesized by ER-associated ribosomes and exported to the Golgi apparatus. Prenylated or acylated peripheral membrane proteins are synthesized in the cytosol and become ER-associated...
  6. Role and Mechanism of Bypass Pathway Trafficking
    Adam D Linstedt; Fiscal Year: 2010
    ..Protein and lipid cargo originating at the cell surface or Golgi apparatus gain access to the pathway by moving to early/sorting endosomes...
  7. Role and Mechanism of Bypass Pathway Trafficking
    Adam Linstedt; Fiscal Year: 2009
    ..Protein and lipid cargo originating at the cell surface or Golgi apparatus gain access to the pathway by moving to early/sorting endosomes...
  8. MOLECULAR STUDIES OF SELECTIVE PROTEIN TRANSPORT
    Gregory Payne; Fiscal Year: 1993
    ..proteins, receptor-mediated endocytosis of the mating peptide alpha-factor, and sorting of proteins from the Golgi apparatus to the lysosome-like vacuole...
  9. ON THE ROLE OF THE HPS GENE PRODUCT IN MELANOCYTES
    Raymond Boissy; Fiscal Year: 2002
    ..In common, these organelles either originate and/or receive specific glycoproteins from the Golgi apparatus. Thus it is hypothesized that the HPS gene product regulates selective trafficking from the trans Golgi ..
  10. MOLECULAR STUDIES OF SELECTIVE PROTEIN TRANSPORT
    Gregory Payne; Fiscal Year: 2000
    ..Together, these approaches will identify previously unrecognized components of the selective protein transport machinery and address the specific roles played by each identified participant. ..
  11. MICROTUBULE-BASED TRANSPORT IN LACRIMAL GLAND FUNCTION
    Sarah F Hamm Alvarez; Fiscal Year: 2010
    ....
  12. Amyloid Beta-Protein: ApoE and Cholesterol Homeostasis
    WELLINGTON WOOD; Fiscal Year: 2009
    ..Determine if the neuronal apoE4 and apoE-null phenotype can be "rescued" by incubation with astrocytes of apoE2, apoE3, or wildtype mice. In some experiments astrocytes will be treated with ABeta1-42. ..
  13. Amyloid Beta-Protein: ApoE and Cholesterol Homeostasis
    WELLINGTON WOOD; Fiscal Year: 2007
    ..Determine if the neuronal apoE4 and apoE-null phenotype can be "rescued" by incubation with astrocytes of apoE2, apoE3, or wildtype mice. In some experiments astrocytes will be treated with ABeta1-42. ..
  14. The Role of Arf GTPases in Endocytosis and Postendocytic Transport
    James E Casanova; Fiscal Year: 2010
    ..It is well established that Arfs promote the assembly of clathrin-coated vesicles in the Golgi apparatus and on endosomes, but their role in clathrin- mediated endocytosis at the plasma membrane is poorly ..
  15. The Role of Arf GTPases in Endocytosis and Postendocytic Transport
    James Casanova; Fiscal Year: 2009
    ..It is well established that Arfs promote the assembly of clathrin-coated vesicles in the Golgi apparatus and on endosomes, but their role in clathrin- mediated endocytosis at the plasma membrane is poorly ..
  16. Intraflagellar Transport Proteins in Mice
    Gregory J Pazour; Fiscal Year: 2011
    ..We have shown that IFT20 is required for ciliary assembly and that it cycles between the Golgi apparatus and the cilium. At the Golgi membrane, IFT20 is complexed with the golgin protein GMAP210...
  17. GLYCOSYLTRANSFERASE FUNCTION DURING FERTILIZATION
    BARRY SHUR; Fiscal Year: 2006
    ..Finally, in the third Aim, we will continue our analysis of GalT-mediated signal transduction leading to the acrosome reaction. ..
  18. SORTING AND TRANSPORT OF YEAST MEMBRANE PROTEINS
    Tom Stevens; Fiscal Year: 2009
    ..Understanding V-ATPase function will provide important insights into diseases of the kidney (renal tubular acidosis), bone diseases (osteopetrosis), and in tumor metastasis, and new drug targets should arise from our studies. ..
  19. SORTING AND TRANSPORT OF YEAST MEMBRANE PROTEINS
    TOM HALL STEVENS; Fiscal Year: 2010
    ..Understanding V-ATPase function will provide important insights into diseases of the kidney (renal tubular acidosis), bone diseases (osteopetrosis), and in tumor metastasis, and new drug targets should arise from our studies. ..
  20. BIOSYNTHESIS AND PROCESSING OF PEPTIDE HORMONE
    Dennis Shields; Fiscal Year: 2007
    ..Aim I1: The Regulation of Golgi PI(4,5)P2 synthesis: Surprisingly, the Golgi apparatus appears to possess little PI(4,5)P2 and its level maybe tightly controlled, in part, by a potent Pl(4,5)P2-5-..
  21. Mannosidase inhibitors as therapeutics for glycoprotein misfolding diseases
    Kelley Moremen; Fiscal Year: 2007
    ....
  22. HUMAN ALKALINE PHYTOCERAMIDASE CONTROL OF ANGIOGENESIS
    Cungui Mao; Fiscal Year: 2002
    ..studies, we demonstrate that haPHC 1) is highly expressed in placenta and heart and localized to the Golgi apparatus and endoplasmic reticulum; 2) hydrolyzes NBD-Clz-phytoceramide preferentially in vitro, and 3) is activated ..
  23. Drs2p Function in Clathrin-coated Vesicle Budding
    TODD GRAHAM; Fiscal Year: 2004
    ..Loss of this asymmetric distribution of PS in blood cells stimulates clot formation and in apoptotic cells allows for recognition and phagocytosis by macrophages. ..
  24. ALPHA-MANNOSIDASES IN MAMMALIAN GLYCOPROTEIN PROCESSING
    Kelley Moremen; Fiscal Year: 1993
    ..Finally, a detailed examination of the expression of the alpha- mannosidases in the context of the glycosylation pathway as a whole should yield a cohesive picture of the coordinated regulation of the pathway in animal systems...
  25. Cyclic AMP Mediation of Epithelial Cell Function
    JAMES RICHARD GOLDENRING; Fiscal Year: 2010
    ..AKAP350 is localized to both centrosomes and the Golgi apparatus, and we have demonstrated that depletion of AKAP350A with siRNA leads to disruption of the Golgi structure as ..