golgi apparatus

Summary

Summary: A stack of flattened vesicles that functions in posttranslational processing and sorting of proteins, receiving them from the rough ENDOPLASMIC RETICULUM and directing them to secretory vesicles, LYSOSOMES, or the CELL MEMBRANE. The movement of proteins takes place by transfer vesicles that bud off from the rough endoplasmic reticulum or Golgi apparatus and fuse with the Golgi, lysosomes or cell membrane. (From Glick, Glossary of Biochemistry and Molecular Biology, 1990)

Top Publications

  1. ncbi Phosphoinositides in cell regulation and membrane dynamics
    Gilbert Di Paolo
    Department of Pathology and Cell Biology, The Taub Institute for Research on Alzheimer s Disease and the Aging Brain, Columbia University Medical Center, New York, New York 10032, USA
    Nature 443:651-7. 2006
  2. ncbi Signals for sorting of transmembrane proteins to endosomes and lysosomes
    Juan S Bonifacino
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Annu Rev Biochem 72:395-447. 2003
  3. pmc ARF family G proteins and their regulators: roles in membrane transport, development and disease
    Julie G Donaldson
    Laboratory of Cell Biology, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Nat Rev Mol Cell Biol 12:362-75. 2011
  4. ncbi COPII and the regulation of protein sorting in mammals
    Giulia Zanetti
    Department of Molecular and Cell Biology and Howard Hughes Medical Institute, University of California at Berkeley, Berkeley, California 94720, USA
    Nat Cell Biol 14:20-8. 2012
  5. ncbi ARF proteins: roles in membrane traffic and beyond
    CRISLYN D'SOUZA-SCHOREY
    Department of Biological Sciences and the Walther Cancer Institute, University of Notre Dame, Notre Dame, Indiana, USA
    Nat Rev Mol Cell Biol 7:347-58. 2006
  6. pmc Cargo-selective endosomal sorting for retrieval to the Golgi requires retromer
    Matthew N J Seaman
    Cambridge Institute for Medical Research, University of Cambridge, Wellcome Trust MRC Building, Addenbrookes Hospital, Hills Road, Cambridge CB2 2XY, England, UK
    J Cell Biol 165:111-22. 2004
  7. ncbi Functions of lipid rafts in biological membranes
    D A Brown
    Department of Biochemistry and Cell Biology, State University of New York at Stony Brook 11794 5215, USA
    Annu Rev Cell Dev Biol 14:111-36. 1998
  8. ncbi Organelle-specific initiation of cell death pathways
    K F Ferri
    Centre National de la Recherche Scientifique, UMR 1599, Institut Gustave Roussy, Pavillon de Recherche 1, 39 rue Camille-Desmoulins, F-94805 Villejuif, France
    Nat Cell Biol 3:E255-63. 2001
  9. ncbi Systematic analysis of SNARE molecules in Arabidopsis: dissection of the post-Golgi network in plant cells
    Tomohiro Uemura
    Graduate School of Biostudies, Kyoto University, Yoshida nihonmatsu, Sakyo ku, Kyoto 606 8501, Japan
    Cell Struct Funct 29:49-65. 2004
  10. ncbi Osh proteins regulate phosphoinositide metabolism at ER-plasma membrane contact sites
    Christopher J Stefan
    Weill Institute for Cell and Molecular Biology, Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA
    Cell 144:389-401. 2011

Detail Information

Publications308 found, 100 shown here

  1. ncbi Phosphoinositides in cell regulation and membrane dynamics
    Gilbert Di Paolo
    Department of Pathology and Cell Biology, The Taub Institute for Research on Alzheimer s Disease and the Aging Brain, Columbia University Medical Center, New York, New York 10032, USA
    Nature 443:651-7. 2006
    ..Their functions, besides classical signal transduction at the cell surface, include regulation of membrane traffic, the cytoskeleton, nuclear events and the permeability and transport functions of membranes...
  2. ncbi Signals for sorting of transmembrane proteins to endosomes and lysosomes
    Juan S Bonifacino
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Annu Rev Biochem 72:395-447. 2003
    ..This complex array of signals and recognition proteins ensures the dynamic but accurate distribution of transmembrane proteins to different compartments of the endosomal-lysosomal system...
  3. pmc ARF family G proteins and their regulators: roles in membrane transport, development and disease
    Julie G Donaldson
    Laboratory of Cell Biology, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Nat Rev Mol Cell Biol 12:362-75. 2011
    ..Important advances are being gained in our understanding of the functional networks that are formed not only by the GEFs and GAPs themselves but also by the inactive forms of the ARF proteins...
  4. ncbi COPII and the regulation of protein sorting in mammals
    Giulia Zanetti
    Department of Molecular and Cell Biology and Howard Hughes Medical Institute, University of California at Berkeley, Berkeley, California 94720, USA
    Nat Cell Biol 14:20-8. 2012
    ..In this review we cover aspects of mammalian COPII-mediated regulation of secretion, in particular related to the function of COPII paralogues, the spatial organization of cargo export and the role of accessory proteins...
  5. ncbi ARF proteins: roles in membrane traffic and beyond
    CRISLYN D'SOUZA-SCHOREY
    Department of Biological Sciences and the Walther Cancer Institute, University of Notre Dame, Notre Dame, Indiana, USA
    Nat Rev Mol Cell Biol 7:347-58. 2006
    ....
  6. pmc Cargo-selective endosomal sorting for retrieval to the Golgi requires retromer
    Matthew N J Seaman
    Cambridge Institute for Medical Research, University of Cambridge, Wellcome Trust MRC Building, Addenbrookes Hospital, Hills Road, Cambridge CB2 2XY, England, UK
    J Cell Biol 165:111-22. 2004
    ..The data presented here support the hypothesis that retromer performs a selective function in endosome-to-Golgi transport, mediating retrieval of the CI-MPR, but not furin...
  7. ncbi Functions of lipid rafts in biological membranes
    D A Brown
    Department of Biochemistry and Cell Biology, State University of New York at Stony Brook 11794 5215, USA
    Annu Rev Cell Dev Biol 14:111-36. 1998
    ..Rafts may also function in sorting and trafficking through the secretory and endocytic pathways...
  8. ncbi Organelle-specific initiation of cell death pathways
    K F Ferri
    Centre National de la Recherche Scientifique, UMR 1599, Institut Gustave Roussy, Pavillon de Recherche 1, 39 rue Camille-Desmoulins, F-94805 Villejuif, France
    Nat Cell Biol 3:E255-63. 2001
    ..evidence suggests that other organelles, including the endoplasmic reticulum (ER), lysosomes and the Golgi apparatus, are also major points of integration of pro-apoptotic signalling or damage sensing...
  9. ncbi Systematic analysis of SNARE molecules in Arabidopsis: dissection of the post-Golgi network in plant cells
    Tomohiro Uemura
    Graduate School of Biostudies, Kyoto University, Yoshida nihonmatsu, Sakyo ku, Kyoto 606 8501, Japan
    Cell Struct Funct 29:49-65. 2004
    ..SNARE proteins were located on specific intracellular compartments: 6 in the endoplasmic reticulum, 9 in the Golgi apparatus, 4 in the trans-Golgi network (TGN), 2 in endosomes, 17 on the plasma membrane, 7 in both the prevacuolar ..
  10. ncbi Osh proteins regulate phosphoinositide metabolism at ER-plasma membrane contact sites
    Christopher J Stefan
    Weill Institute for Cell and Molecular Biology, Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA
    Cell 144:389-401. 2011
    ..Our findings further suggest that the conserved Osh proteins control PI metabolism at additional membrane contact sites...
  11. pmc Furin at the cutting edge: from protein traffic to embryogenesis and disease
    Gary Thomas
    Vollum Institute, 3181 SW Sam Jackson Park Road, Portland, Oregon 97239, USA
    Nat Rev Mol Cell Biol 3:753-66. 2002
    ..This review summarizes various features of furin--its structural and enzymatic properties, intracellular localization, trafficking, substrates, and roles in vivo...
  12. pmc A comprehensive comparison of transmembrane domains reveals organelle-specific properties
    Hayley J Sharpe
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, UK
    Cell 142:158-69. 2010
    ..The pervasive presence of organelle-specific features among the TMDs of a particular organelle has implications for TMD prediction, regulation of protein activity by location, and sorting of proteins and lipids in the secretory pathway...
  13. pmc Characterization of a cis-Golgi matrix protein, GM130
    N Nakamura
    Cell Biology Laboratory, Imperial Cancer Research Fund, London, UK
    J Cell Biol 131:1715-26. 1995
    ..Together these results suggest that GM130 is part of a cis-Golgi matrix and has a role in maintaining cis-Golgi structure...
  14. pmc A membrane coat complex essential for endosome-to-Golgi retrograde transport in yeast
    M N Seaman
    Division of Cellular and Molecular Medicine and Howard Hughes Medical Institute, University of California at San Diego, School of Medicine, La Jolla, California 92093 0668, USA
    J Cell Biol 142:665-81. 1998
    ..Conservation of the yeast retromer complex components in higher eukaryotes suggests an important general role for this complex in endosome-to-Golgi retrieval...
  15. ncbi Sar1p N-terminal helix initiates membrane curvature and completes the fission of a COPII vesicle
    Marcus C S Lee
    Department of Molecular and Cell Biology and Howard Hughes Medical Institute, University of California, Berkeley, California 94702, USA
    Cell 122:605-17. 2005
    ..Thus, the initiation of vesicle budding by Sar1p couples the generation of membrane curvature with coat-protein assembly and cargo capture...
  16. ncbi Golgi maturation visualized in living yeast
    Eugene Losev
    Department of Molecular Genetics and Cell Biology, and Institute for Biophysical Dynamics, The University of Chicago, 920 East 58th Street, Chicago, Illinois 60637, USA
    Nature 441:1002-6. 2006
    The Golgi apparatus is composed of biochemically distinct early (cis, medial) and late (trans, TGN) cisternae. There is debate about the nature of these cisternae...
  17. pmc Alpha-synuclein blocks ER-Golgi traffic and Rab1 rescues neuron loss in Parkinson's models
    Antony A Cooper
    School of Biological Sciences, University of Missouri Kansas City, Kansas City, MO 64110, USA
    Science 313:324-8. 2006
    ..Thus, synucleinopathies may result from disruptions in basic cellular functions that interface with the unique biology of particular neurons to make them especially vulnerable...
  18. pmc Models for Golgi traffic: a critical assessment
    Benjamin S Glick
    Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, Illinois 60637, USA
    Cold Spring Harb Perspect Biol 3:a005215. 2011
    ....
  19. ncbi Intracellular functions of N-linked glycans
    A Helenius
    Institute of Biochemistry, , , , Switzerland
    Science 291:2364-9. 2001
    ..The division of synthesis and processing between the ER and the Golgi complex represents an evolutionary adaptation that allows efficient exploitation of the potential of oligosaccharides...
  20. pmc NKG2D ligand MICA is retained in the cis-Golgi apparatus by human cytomegalovirus protein UL142
    Omodele Ashiru
    Department of Pathology, Cambridge Institute for Medical Research, University of Cambridge, Cambridge, United Kingdom
    J Virol 83:12345-54. 2009
    ..Here, we show that UL142 localizes predominantly to the endoplasmic reticulum (ER) and cis-Golgi apparatus. The transmembrane domain of UL142 mediates its ER localization, while we propose that the UL142 luminal ..
  21. pmc Identification of a family of animal sphingomyelin synthases
    Klazien Huitema
    Department of Membrane Enzymology, Faculty of Chemistry, Institute of Biomembranes, Utrecht University, Utrecht, The Netherlands
    EMBO J 23:33-44. 2004
    ..Whereas human SMS1 is localised to the Golgi, SMS2 resides primarily at the plasma membrane. Collectively, these findings open up important new avenues for studying sphingolipid function in animals...
  22. ncbi Ras proteins: different signals from different locations
    John F Hancock
    Institute for Molecular Bioscience and Department of Molecular and Cellular Pathology, University of Queensland, Brisbane, Australia 4072
    Nat Rev Mol Cell Biol 4:373-84. 2003
    ..These different membrane microenvironments modulate Ras signal output and highlight the complex interplay between Ras location and function...
  23. pmc Sedlin controls the ER export of procollagen by regulating the Sar1 cycle
    Rossella Venditti
    Telethon Institute of Genetics and Medicine, Naples, Italy
    Science 337:1668-72. 2012
    ..This joint action of TANGO1 and Sedlin sustained the ER export of PC, and its derangement may explain the defective chondrogenesis underlying SEDT...
  24. ncbi SEC7 encodes an unusual, high molecular weight protein required for membrane traffic from the yeast Golgi apparatus
    T Achstetter
    Department of Biochemistry, University of California, Berkeley 94720
    J Biol Chem 263:11711-7. 1988
    ..cerevisiae with mutations at the sec7 locus are pleiotropically deficient in protein transport within the Golgi apparatus and proliferate a large array of Golgi cisternae at a restrictive growth temperature (37 degrees C)...
  25. pmc PtdIns4P recognition by Vps74/GOLPH3 links PtdIns 4-kinase signaling to retrograde Golgi trafficking
    Christopher S Wood
    Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, 19104, USA
    J Cell Biol 187:967-75. 2009
    Targeting and retention of resident integral membrane proteins of the Golgi apparatus underly the function of the Golgi in glycoprotein and glycolipid processing and sorting...
  26. pmc Role of oxysterol binding protein in hepatitis C virus infection
    Yutaka Amako
    Department of Medicine, University of California, San Diego, La Jolla, 92093, USA
    J Virol 83:9237-46. 2009
    ..The pleckstrin homology (PH) domain located in the N-terminal region of OSBP targeted this protein to the Golgi apparatus. OSBP deletion mutation in the PH (DeltaPH) domain failed to localize to the Golgi apparatus and inhibited ..
  27. pmc Coatomer-dependent protein delivery to lipid droplets
    Krishnakant G Soni
    Cell Biology and Metabolism Program, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    J Cell Sci 122:1834-41. 2009
    ..Here we demonstrate that ATGL and ADRP, but not TIP47, are delivered to LDs by a pathway mediated by the COPI and COPII coatomer proteins and their corresponding regulators...
  28. ncbi ADP-ribosylation factors (ARFs) and ARF-like 1 (ARL1) have both specific and shared effectors: characterizing ARL1-binding proteins
    H Van Valkenburgh
    Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia 30322 3050, USA
    J Biol Chem 276:22826-37. 2001
    ..These data reveal a complex network of interactions between GTPases in the ARF family and their effectors and reveal a potential for cross-talk not demonstrated previously...
  29. pmc Asymmetric CLASP-dependent nucleation of noncentrosomal microtubules at the trans-Golgi network
    Andrey Efimov
    Department of Cell and Developmental Biology, Vanderbilt University Medical Center, Nashville, TN 37232, USA
    Dev Cell 12:917-30. 2007
    ..Here we demonstrate that a large number of microtubules in untreated human cells originate from the Golgi apparatus in a centrosome-independent manner...
  30. pmc Assembly and maturation of the flavivirus Kunjin virus appear to occur in the rough endoplasmic reticulum and along the secretory pathway, respectively
    J M MacKenzie
    Clinical Medical Virology Centre, University of Queensland, St Lucia, Queensland 4072, Australia
    J Virol 75:10787-99. 2001
    ..virions from the RER involves the transport of individual virions within individual vesicles en route to the Golgi apparatus. The results suggest that the assembly of virions occurs within the lumen of the RER and that subsequent ..
  31. pmc Exit from the Golgi is required for the expansion of the autophagosomal phagophore in yeast Saccharomyces cerevisiae
    Aniek van der Vaart
    Department of Cell Biology and Institute of Biomembranes, University Medical Center Utrecht, Utrecht 3584, The Netherlands
    Mol Biol Cell 21:2270-84. 2010
    ..Our data show that the Golgi complex plays a crucial role in supplying the lipid bilayers necessary for the biogenesis of double-membrane vesicles possibly through a new class of transport carriers or a new mechanism...
  32. pmc PTRF-Cavin, a conserved cytoplasmic protein required for caveola formation and function
    Michelle M Hill
    Institute for Molecular Bioscience, University of Queensland, Brisbane, Queensland 4072, Australia
    Cell 132:113-24. 2008
    ..We conclude that PTRF-Cavin is required for caveola formation and sequestration of mobile caveolin into immobile caveolae...
  33. pmc Membrane lipids: where they are and how they behave
    Gerrit van Meer
    Bijvoet Center and Institute of Biomembranes, Utrecht University, 3584 CH Utrecht, The Netherlands
    Nat Rev Mol Cell Biol 9:112-24. 2008
    ..enzymes, translocases and transporters, plus the intrinsic physical phase behaviour of lipids and their interactions with membrane proteins, to create the unique compositions and multiple functionalities of their individual membranes?..
  34. ncbi Centrosome localization determines neuronal polarity
    Froylan Calderon de Anda
    Cavalieri Ottolenghi Scientific Institute, Universita degli Studi di Torino, 10043 Orbassano, Torino, Italy
    Nature 436:704-8. 2005
    ..Centrosomes, the Golgi apparatus and endosomes cluster together close to the area where the first neurite will form, which is in turn opposite ..
  35. pmc Polarized insertion of new membrane from a cytoplasmic reservoir during cleavage of the Drosophila embryo
    T Lecuit
    Howard Hughes Medical Institute, Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA
    J Cell Biol 150:849-60. 2000
    ..Depolymerization of microtubules inhibits the export of a newly synthesized transmembrane protein from the Golgi apparatus to the plasma membrane and simultaneously blocks membrane growth...
  36. pmc Recycling of golgi-resident glycosyltransferases through the ER reveals a novel pathway and provides an explanation for nocodazole-induced Golgi scattering
    B Storrie
    Department of Biochemistry, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061 0308, USA
    J Cell Biol 143:1505-21. 1998
    During microtubule depolymerization, the central, juxtanuclear Golgi apparatus scatters to multiple peripheral sites...
  37. ncbi Phosphatidylinositol 4 phosphate regulates targeting of clathrin adaptor AP-1 complexes to the Golgi
    Ying Jie Wang
    Department of Physiology, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390, USA
    Cell 114:299-310. 2003
    ..We propose that PI4KIIalpha establishes the Golgi's unique lipid-defined organelle identity by generating PI(4)P-rich domains that specify the docking of the AP-1 coat machinery...
  38. pmc COPI acts in both vesicular and tubular transport
    Jia Shu Yang
    Division of Rheumatology, Immunology and Allergy, Brigham and Women s Hospital, and Department of Medicine, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nat Cell Biol 13:996-1003. 2011
    ....
  39. ncbi Ras signalling on the endoplasmic reticulum and the Golgi
    Vi K Chiu
    Department of Medicine, Cell Biology and Pharmacology, New York University School of Medicine, 550 First Avenue, New York, NY 10016, USA
    Nat Cell Biol 4:343-50. 2002
    ..Thus, Ras signalling is not limited to the PM, but also proceeds on the endomembrane...
  40. pmc Global detection of protein kinase D-dependent phosphorylation events in nocodazole-treated human cells
    Mirita Franz-Wachtel
    Proteome Center Tuebingen, University of Tuebingen, Tuebingen, Germany
    Mol Cell Proteomics 11:160-70. 2012
    ..This study presents the first global detection of PKD1-dependent phosphorylation events and provides a wealth of information for functional follow-up of PKD1 activity upon disruption of the Golgi network in human cells...
  41. pmc RNAi screening reveals a large signaling network controlling the Golgi apparatus in human cells
    Joanne Chia
    Laboratory Regulation of Membrane Traffic, Institute of Molecular and Cell Biology, Singapore, Singapore
    Mol Syst Biol 8:629. 2012
    The Golgi apparatus has many important physiological functions, including sorting of secretory cargo and biosynthesis of complex glycans. These functions depend on the intricate and compartmentalized organization of the Golgi apparatus...
  42. ncbi Rab and actomyosin-dependent fission of transport vesicles at the Golgi complex
    S Miserey-Lenkei
    Institut Curie, CNRS UMR144, Molecular mechanisms of intracellular transport Lab, 26 Rue d Ulm, 75248 Paris Cedex 05, France
    Nat Cell Biol 12:645-54. 2010
    ..Our results provide evidence that the actomyosin system is required in vesicle biogenesis at the Golgi, and uncover a function for Rab GTPases in vesicle fission...
  43. ncbi The multiple facets of the Golgi reassembly stacking proteins
    Fabian P Vinke
    Cell Microscopy Centre, Department of Cell Biology, UMC Utrecht, The Netherlands
    Biochem J 433:423-33. 2011
    ..In the present paper, we review the multiple roles of GRASPs...
  44. pmc Electron tomography reveals Rab6 is essential to the trafficking of trans-Golgi clathrin and COPI-coated vesicles and the maintenance of Golgi cisternal number
    Brian Storrie
    Department of Physiology and Biophysics, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA
    Traffic 13:727-44. 2012
    ....
  45. pmc Arfaptins are localized to the trans-Golgi by interaction with Arl1, but not Arfs
    Zhiqiu Man
    Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto, Japan
    J Biol Chem 286:11569-78. 2011
    ..These observations indicate that arfaptins are recruited onto trans-Golgi membranes by interacting with Arl1, and capable of inducing membrane deformation via their BAR domains...
  46. ncbi A combinatorial genetic library approach to target heterologous glycosylation enzymes to the endoplasmic reticulum or the Golgi apparatus of Pichia pastoris
    Juergen H Nett
    GlycoFi Inc, 21 Lafayette Street, Suite 200, Lebanon, NH 03766, USA
    Yeast 28:237-52. 2011
    ..This combinatorial approach, together with a high-throughput screening protocol, has allowed us to humanize the yeast glycosylation pathway to secrete human glycoprotein with complex N-glycosylation...
  47. pmc Targeting of a chlamydial protease impedes intracellular bacterial growth
    Jan G Christian
    Institute for Medical Microbiology, Immunology and Hygiene, Technische Universitat Munchen, Munich, Germany
    PLoS Pathog 7:e1002283. 2011
    ..Recent work has shown that growth of Chlamydia induces the fragmentation of the Golgi apparatus (GA) into ministacks, which facilitates the acquisition of host lipids into the growing inclusion...
  48. pmc Endosome to Golgi retrieval of the vacuolar protein sorting receptor, Vps10p, requires the function of the VPS29, VPS30, and VPS35 gene products
    M N Seaman
    Division of Cellular and Molecular Medicine, University of California, San Diego, School of Medicine, La Jolla 92093 0668, USA
    J Cell Biol 137:79-92. 1997
    ....
  49. pmc Rab proteins mediate Golgi transport of caveola-internalized glycosphingolipids and correct lipid trafficking in Niemann-Pick C cells
    Amit Choudhury
    Department of Biochemistry and Molecular Biology, Thoracic Diseases Research Unit, Mayo Clinic and Foundation, Rochester, Minnesota 55905, USA
    J Clin Invest 109:1541-50. 2002
    ..Our results demonstrate a role for Rab7 and Rab9 in the Golgi targeting of glycosphingolipids and suggest a new therapeutic approach for restoring normal lipid trafficking in NP-C cells...
  50. pmc A Rab GAP cascade defines the boundary between two Rab GTPases on the secretory pathway
    Félix E Rivera-Molina
    Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06520, USA
    Proc Natl Acad Sci U S A 106:14408-13. 2009
    ..GEF cascades working in concert with counter-current GAP cascades could generate a programmed series of Rab conversions responsible for regulating the choreography of membrane traffic...
  51. ncbi A molecular network for the transport of the TI-VAMP/VAMP7 vesicles from cell center to periphery
    Andrea Burgo
    Institut Jacques Monod, UMR 7592, CNRS, Universite Paris Diderot, Sorbonne Paris Cité, Paris, France
    Dev Cell 23:166-80. 2012
    ..The molecular mechanisms uncovered here suggest an integrated view of the transport of vesicles carrying a specific v-SNARE toward the cell surface...
  52. ncbi The COPI system: molecular mechanisms and function
    R Beck
    Yale University School of Medicine, Department of Cell Biology, New Haven, CT 06520, USA
    FEBS Lett 583:2701-9. 2009
    ..Specifically, we will weigh the pros and cons of recent data on roles of the small GTP binding protein Arf1, of Arf1GAPs, and lipids during COPI carrier formation...
  53. ncbi TBC1D20 is a Rab1 GTPase-activating protein that mediates hepatitis C virus replication
    Ella H Sklan
    Department of Medicine, Division of Gastroenterology and Hepatology, Stanford University School of Medicine, Palo Alto, CA 94305 5187, USA
    J Biol Chem 282:36354-61. 2007
    ..These results highlight a novel mechanism by which viruses can hijack host cell machinery and suggest an attractive model whereby the NS5A-TBC1D20 interaction may promote viral membrane-associated RNA replication...
  54. pmc GOLPH3 bridges phosphatidylinositol-4- phosphate and actomyosin to stretch and shape the Golgi to promote budding
    Holly C Dippold
    Division of Endocrinology and Metabolism, Department of Medicine, University of California, San Diego, La Jolla, CA 92093 0707, USA
    Cell 139:337-51. 2009
    ..Consequently, this tensile force stretches the Golgi into the extended ribbon observed by fluorescence microscopy and the familiar flattened form observed by electron microscopy...
  55. ncbi The human phosphatidylinositol phosphatase SAC1 interacts with the coatomer I complex
    Holger M Rohde
    Boehringer Ingelheim Austria GmbH, Dr Boehringer Gasse 5 11, 1121 Vienna, Austria
    J Biol Chem 278:52689-99. 2003
    ..cerevisiae SAC1 gene encodes an integral membrane protein of the endoplasmic reticulum (ER) and the Golgi apparatus. Yeast SAC1 mutants display a wide array of phenotypes including inositol auxotrophy, cold sensitivity, ..
  56. pmc The Parkinson's disease protein alpha-synuclein disrupts cellular Rab homeostasis
    Aaron D Gitler
    Whitehead Institute for Biomedical Research and Howard Hughes Medical Institute, Cambridge, MA 02142, USA
    Proc Natl Acad Sci U S A 105:145-50. 2008
    ..Thus, alpha-syn causes general defects in vesicle trafficking, to which dopaminergic neurons are especially sensitive...
  57. ncbi Structural organization of the Golgi apparatus
    Martin Lowe
    Faculty of Life Sciences, University of Manchester, The Michael Smith Building, Oxford Road, Manchester M13 9PT, UK
    Curr Opin Cell Biol 23:85-93. 2011
    The Golgi apparatus is a universal feature of eukaryotes, carrying out the key functions of processing, sorting and trafficking of newly synthesized membrane and secretory proteins...
  58. pmc Secreted kinase phosphorylates extracellular proteins that regulate biomineralization
    Vincent S Tagliabracci
    Department of Pharmacology, University of California, San Diego, La Jolla, CA 92093 0721, USA
    Science 336:1150-3. 2012
    ..We identified a family of atypical protein kinases that localize within the Golgi apparatus and are secreted...
  59. ncbi TGN golgins, Rabs and cytoskeleton: regulating the Golgi trafficking highways
    Bruno Goud
    Institut Curie, CNRS UMR144, 26, rue d Ulm, Paris cedex 75248, France
    Trends Cell Biol 20:329-36. 2010
    The architecture of the Golgi apparatus is intimately linked to its role in regulating membrane trafficking...
  60. ncbi Multiple cytosolic and transmembrane determinants are required for the trafficking of SCAMP1 via an ER-Golgi-TGN-PM pathway
    Yi Cai
    School of Life Sciences, Centre for Cell and Developmental Biology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China
    Plant J 65:882-96. 2011
    ..Therefore, both the cytosolic N-terminus and TMD sequences of SCAMP1 play integral roles in mediating its transport to the PM via an ER-Golgi-TGN pathway...
  61. ncbi Mutations in dynein link motor neuron degeneration to defects in retrograde transport
    Majid Hafezparast
    Department of Neurodegenerative Disease, Institute of Neurology, National Hospital for Neurology and Neurosurgery, Queen Square, London WC1N 3BG, UK
    Science 300:808-12. 2003
    ..These mutations exclusively perturb neuron-specific functions of dynein...
  62. ncbi Vesicular transport: the core machinery of COPI recruitment and budding
    Walter Nickel
    Biochemie Zentrum Heidelberg, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany
    J Cell Sci 115:3235-40. 2002
    ..The new findings have allowed us to generate a model that covers the cycle of coat recruitment, coat polymerization, vesicle budding and uncoating...
  63. ncbi Quality control in the endoplasmic reticulum
    Lars Ellgaard
    Institute of Biochemistry, Swiss Federal Institute of Technology ETH Zurich, Honggerberg, CH 8093 Zurich, Switzerland
    Nat Rev Mol Cell Biol 4:181-91. 2003
    ..Here, we discuss recent progress in understanding the conformation-specific sorting of proteins at the level of ER retention and export...
  64. ncbi Production of complex human glycoproteins in yeast
    Stephen R Hamilton
    Thayer School of Engineering and the Department of Biological Sciences, Dartmouth College, Hanover, NH 03755, USA
    Science 301:1244-6. 2003
    ....
  65. pmc Orm1 and Orm2 are conserved endoplasmic reticulum membrane proteins regulating lipid homeostasis and protein quality control
    Sumin Han
    Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA
    Proc Natl Acad Sci U S A 107:5851-6. 2010
    ..Thus, the yeast Orm proteins control membrane biogenesis by coordinating lipid homeostasis with protein quality control...
  66. ncbi Live imaging of yeast Golgi cisternal maturation
    Kumi Matsuura-Tokita
    Molecular Membrane Biology Laboratory, RIKEN Discovery Research Institute, Wako, Saitama 351 0198, Japan
    Nature 441:1007-10. 2006
    There is a debate over how protein trafficking is performed through the Golgi apparatus. In the secretory pathway, secretory proteins that are synthesized in the endoplasmic reticulum enter the early compartment of the Golgi apparatus ..
  67. ncbi Secretory COPII coat component Sec23a is essential for craniofacial chondrocyte maturation
    Michael R Lang
    Department of Medicine, Division of Genetic Medicine, Vanderbilt University Medical Center, Nashville, Tennessee 37232, USA
    Nat Genet 38:1198-203. 2006
    ..of the COPII complex, which is critical for anterograde protein trafficking between endoplasmic reticulum and Golgi apparatus. Zebrafish crusher mutants develop normally until the onset of craniofacial chondrogenesis...
  68. pmc Golgi-derived CLASP-dependent microtubules control Golgi organization and polarized trafficking in motile cells
    Paul M Miller
    Department of Cell and Developmental Biology, Vanderbilt University Medical Center, Nashville, TN 37232, USA
    Nat Cell Biol 11:1069-80. 2009
    ....
  69. ncbi Analysis of GTPase-activating proteins: Rab1 and Rab43 are key Rabs required to maintain a functional Golgi complex in human cells
    Alexander K Haas
    Cancer Research Centre, University of Liverpool, 200 London Road, Liverpool, L9 3AT, UK
    J Cell Sci 120:2997-3010. 2007
    ....
  70. pmc Exocytosis of post-Golgi vesicles is regulated by components of the endocytic machinery
    Jyoti K Jaiswal
    The Rockefeller University, 1230 York Avenue, Box 304, New York, NY 10065, USA
    Cell 137:1308-19. 2009
    ..Our results demonstrate that these components of the endocytic machinery modulate the nature and extent of biosynthetic cargo delivery by post-Golgi vesicles at the cell membrane...
  71. ncbi TANGO1 facilitates cargo loading at endoplasmic reticulum exit sites
    Kota Saito
    Department of Cell and Developmental Biology, CRG Centre de Regulacio Genomica, Barcelona, Spain
    Cell 136:891-902. 2009
    ..We propose that TANGO1 exports other cargoes in cells that do not secrete collagen VII. However, TANGO1 does not enter the budding carrier, which represents a unique mechanism to load cargo into COPII carriers...
  72. ncbi Chlamydia causes fragmentation of the Golgi compartment to ensure reproduction
    Dagmar Heuer
    Department of Molecular Biology, Max Planck Institute for Infection Biology, Chariteplatz 1, 10117 Berlin, Germany
    Nature 457:731-5. 2009
    ..for host cell lipids, such as sphingolipids and cholesterol, produced in the endoplasmic reticulum and the Golgi apparatus. However, the mechanisms by which intracellular pathogens acquire host cell lipids are not well understood...
  73. pmc Electrospray ionization tandem mass spectrometry (ESI-MS/MS) analysis of the lipid molecular species composition of yeast subcellular membranes reveals acyl chain-based sorting/remodeling of distinct molecular species en route to the plasma membrane
    R Schneiter
    Spezialforschungsbereich Biomembrane Research Center, Institut fur Biochemie und Lebensmittelchemie, Technische Universitat Graz, A 8010 Graz, Austria
    J Cell Biol 146:741-54. 1999
    ..This observation suggests that acyl chain-based sorting and/or remodeling mechanisms are operating to maintain the specific lipid molecular species composition of the yeast plasma membrane...
  74. pmc Caspase-2 is localized at the Golgi complex and cleaves golgin-160 during apoptosis
    M Mancini
    Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Cell Biol 149:603-12. 2000
    ..We propose that the Golgi complex, like mitochondria, senses and integrates unique local conditions, and transduces pro-apoptotic signals through local caspases, which regulate local effectors...
  75. pmc The Golgi-associated hook3 protein is a member of a novel family of microtubule-binding proteins
    J H Walenta
    Center for Basic Neuroscience and Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    J Cell Biol 152:923-34. 2001
    ..Because overexpression of Hook3 caused fragmentation of the Golgi complex, we propose that Hook3 participates in defining the architecture and localization of the mammalian Golgi complex...
  76. ncbi Compartmental specificity of cellular membrane fusion encoded in SNARE proteins
    J A McNew
    Cellular Biochemistry and Biophysics Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Nature 407:153-9. 2000
    ..Here we find that, to a marked degree, the pattern of membrane flow in the cell is encoded and recapitulated by its isolated SNARE proteins, as predicted by the SNARE hypothesis...
  77. ncbi Syntaxin 18, a SNAP receptor that functions in the endoplasmic reticulum, intermediate compartment, and cis-Golgi vesicle trafficking
    K Hatsuzawa
    School of Life Science, Tokyo University of Pharmacy and Life Science, Hachioji, Tokyo 192 0392, Japan
    J Biol Chem 275:13713-20. 2000
    ..Moreover, overexpression of the FLAG-syntaxin 18 mutant inhibited protein export from the endoplasmic reticulum. These results taken together suggest that syntaxin 18 functions in transport between the endoplasmic reticulum and Golgi...
  78. ncbi Interaction of tyrosine-based sorting signals with clathrin-associated proteins
    H Ohno
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Science 269:1872-5. 1995
    ..The interaction was confirmed by in vitro binding assays. Thus, it is likely that the medium chains serve as signal-binding components of the clathrin-dependent sorting machinery...
  79. pmc A comparative study of the involvement of 17 Arabidopsis myosin family members on the motility of Golgi and other organelles
    Dror Avisar
    Institute of Plant Sciences, Volcani Center, Bet Dagan 50250, Israel
    Plant Physiol 150:700-9. 2009
    ..Taken together, our data suggest that the above myosins are involved, directly or indirectly, in the movement of Golgi and mitochondria in plant cells...
  80. ncbi Exiting the Golgi complex
    Maria Antonietta De Matteis
    Department of Cell Biology and Oncology, Consorzio Mario Negri Sud, 66030 Santa Maria Imbaro CH, Italy
    Nat Rev Mol Cell Biol 9:273-84. 2008
    ....
  81. pmc Arf6 and microtubules in adhesion-dependent trafficking of lipid rafts
    Nagaraj Balasubramanian
    Robert M Beirne Cardiovascular Research Center, Mellon Prostate Cancer Research Institute, University of Virginia, Charlottesville, Virginia 22908, USA
    Nat Cell Biol 9:1381-91. 2007
    ..Both of these steps are required for the plasma membrane targeting of Rac1 and for its activation. These data therefore define a new membrane raft trafficking pathway that is crucial for anchorage-dependent signalling...
  82. pmc Coordinated lipid transfer between the endoplasmic reticulum and the Golgi complex requires the VAP proteins and is essential for Golgi-mediated transport
    Diego Peretti
    The Molecular Cell Biology Department, Weizmann Institute of Science, Rehovot 76100, Israel
    Mol Biol Cell 19:3871-84. 2008
    ..We propose that Nir2, OSBP, and CERT function coordinately at the ER-Golgi membrane contact sites, thereby affecting the lipid composition of the Golgi membranes and consequently their structural and functional identities...
  83. pmc Loss of Apm1, the micro1 subunit of the clathrin-associated adaptor-protein-1 complex, causes distinct phenotypes and synthetic lethality with calcineurin deletion in fission yeast
    Ayako Kita
    Division of Molecular Pharmacology and Pharmacogenomics, Department of Genome Sciences, Kobe University Graduate School of Medicine, Kobe 650 0017, Japan
    Mol Biol Cell 15:2920-31. 2004
    ....
  84. ncbi Functional interplay between LIS1, NDE1 and NDEL1 in dynein-dependent organelle positioning
    Connie Lam
    Faculty of Life Sciences, University of Manchester, Manchester, M13 9PT, UK
    J Cell Sci 123:202-12. 2010
    ..Consistent with this hypothesis, expression of exogenous NDE1 or NDEL1 rescued the effects of LIS1 depletion on Golgi organisation, whereas LIS1 was only partially effective at rescuing the loss of NDE1 and NDEL1...
  85. ncbi The palmitoylation machinery is a spatially organizing system for peripheral membrane proteins
    Oliver Rocks
    European Molecular Biology Laboratory, Meyerhofstrasse 1, 69118 Heidelberg, Germany
    Cell 141:458-71. 2010
    ..This generic spatially organizing system differs from conventional receptor-mediated targeting mechanisms and efficiently counteracts entropy-driven redistribution of palmitoylated peripheral membrane proteins over all membranes...
  86. pmc Nanoscale architecture of endoplasmic reticulum export sites and of Golgi membranes as determined by electron tomography
    L Andrew Staehelin
    Molecular Cellular and Developmental Biology, University of Colorado, Boulder, Colorado 80309, USA
    Plant Physiol 147:1454-68. 2008
  87. ncbi A Golgi-associated PDZ domain protein modulates cystic fibrosis transmembrane regulator plasma membrane expression
    Jie Cheng
    Department of Physiology and Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Biol Chem 277:3520-9. 2002
    ..CAL is located primarily at the Golgi apparatus, co-localizing with trans-Golgi markers and is sensitive to Brefeldin A treatment...
  88. ncbi The N-terminal pro region mediates retention of unprocessed type-I PME in the Golgi apparatus
    Sebastian Wolf
    Heidelberg Institute for Plant Sciences, INF 360, 69120 Heidelberg, Germany
    Plant J 58:361-75. 2009
    ..indicate that the pro region operates as an effective retention mechanism, keeping unprocessed PME in the Golgi apparatus. Consequently, pro-protein processing could constitute a post-translational mechanism regulating PME activity.
  89. ncbi Deletion mutants of AP-1 adaptin subunits display distinct phenotypes in fission yeast
    Yan Ma
    Division of Molecular Pharmacology and Pharmacogenomics, Department of Biochemistry and Molecular Biology, Kobe University Graduate School of Medicine, Kusunoki cho, Chuo Ku, Kobe 650 0017, Japan
    Genes Cells 14:1015-28. 2009
    ..Altogether, results suggest that the four subunits are all essential for the heterotetrameric complex formation and for the AP-1 function in exit transport from endosomes...
  90. pmc Dissection of Arabidopsis ADP-RIBOSYLATION FACTOR 1 function in epidermal cell polarity
    Jian Xu
    Department of Molecular Cell Biology, Utrecht University, 3584 CH Utrecht, The Netherlands
    Plant Cell 17:525-36. 2005
    ..Fluorescence colocalization studies reveal that ARF1 is localized to the Golgi apparatus and endocytic organelles in both onion (Allium cepa) and Arabidopsis cells...
  91. ncbi Golgi recruitment of GRIP domain proteins by Arf-like GTPase 1 is regulated by Arf-like GTPase 3
    Subba Rao Gangi Setty
    University of Pennsylvania School of Medicine, Department of Cell and Developmental Biology, 421 Curie Boulevard, BRB 2 3, Room 1010, Philadelphia, PA 19104 6058, USA
    Curr Biol 13:401-4. 2003
    ..The similar requirements for localization of GRIP domains from yeast, mouse, and human when expressed in yeast, and the presence of Arl1p and Arl3p homologs in these species, suggest that this is an evolutionarily conserved mechanism...
  92. pmc A missense mutation in the Arabidopsis COPII coat protein Sec24A induces the formation of clusters of the endoplasmic reticulum and Golgi apparatus
    Carmen Faso
    Department of Energy, Plant Research Laboratory, Michigan State University, East Lansing, MI 48824, USA
    Plant Cell 21:3655-71. 2009
    How the endoplasmic reticulum (ER) and the Golgi apparatus maintain their morphological and functional identity while working in concert to ensure the production of biomolecules necessary for the cell's survival is a fundamental question ..
  93. pmc The destination for single-pass membrane proteins is influenced markedly by the length of the hydrophobic domain
    Federica Brandizzi
    Research School of Biological and Molecular Sciences, Oxford Brookes University, Gipsy Lane Campus, OX3 0BP Headington Oxford, United Kingdom
    Plant Cell 14:1077-92. 2002
    ..of the TM20-labeled compartments was investigated by coexpression with a marker localized mainly in the Golgi apparatus, AtERD2, fused to a yellow fluorescent protein...
  94. pmc Regulation of vascular endothelial growth factor receptor 2 trafficking and angiogenesis by Golgi localized t-SNARE syntaxin 6
    Venkatraman Manickam
    Department of Anatomy and Cell Biology, University of Iowa, 51 Newton Rd, Iowa City, IA 52242, USA
    Blood 117:1425-35. 2011
    ..Plasma membrane (PM) levels of VEGFR2 are regulated by endocytosis and secretory transport through the Golgi apparatus. To date, the mechanism whereby the VEGFR2 traffics through the Golgi apparatus remains incompletely ..
  95. ncbi Signalling to and from the secretory pathway
    Hesso Farhan
    Biozentrum, University of Basel, Klingelbergstrasse 50 70, Basel, Switzerland
    J Cell Sci 124:171-80. 2011
    ..This Commentary highlights recent contributions to our understanding of the mutual regulation of the secretory pathway and cellular signalling...
  96. pmc Is the 6 kDa tobacco etch viral protein a bona fide ERES marker?
    Alexander Lerich
    Department of Plant Cell Biology, Centre for Organismal Studies, University of Heidelberg, Germany
    J Exp Bot 62:5013-23. 2011
    ..However, based on a consideration of the structure of its cytoplasmic tail, the scenario that the VP collects at ERES and is transported to the cis-Golgi before being recycled back to the ER, is supported...
  97. pmc Phosphoinositide 3-kinase δ regulates membrane fission of Golgi carriers for selective cytokine secretion
    Pei Ching Low
    Institute for Molecular Bioscience, University of Queensland, Brisbane, Queensland 4072, Australia
    J Cell Biol 190:1053-65. 2010
    ..These findings demonstrate a new function for p110δ as part of the membrane fission machinery required at the TGN for the selective trafficking and secretion of cytokines in macrophages...
  98. pmc Impact on the endoplasmic reticulum and Golgi apparatus of turnip mosaic virus infection
    Romain Grangeon
    INRS Institut Armand Frappier, Institut National de la Recherche Scientifique, Laval, Quebec, Canada
    J Virol 86:9255-65. 2012
    ..TuMV infection led to the amalgamation of the endoplasmic reticulum (ER), Golgi apparatus, COPII coatamers, and chloroplasts into a perinuclear globular structure that also contained viral proteins...
  99. pmc The rice alpha-amylase glycoprotein is targeted from the Golgi apparatus through the secretory pathway to the plastids
    Aya Kitajima
    Graduate School of Science and Technology, Niigata University, Niigata, Japan
    Plant Cell 21:2844-58. 2009
    ..Thus, Golgi-to-plastid traffic appears to be involved in the transport of glycoproteins to plastids and plastid targeting seems to be accomplished in a sorting signal-dependent manner...
  100. pmc Salt tolerance of Arabidopsis thaliana requires maturation of N-glycosylated proteins in the Golgi apparatus
    Jae Sook Kang
    Brain Korea 21, Division of Applied Life Science and Environmental Biotechnology National Core Research Center, Graduate School of Gyeongsang National University, Jinju 660 701, Korea
    Proc Natl Acad Sci U S A 105:5933-8. 2008
    Protein N-glycosylation in the endoplasmic reticulum (ER) and in the Golgi apparatus is an essential process in eukaryotic cells...
  101. ncbi Protein sorting to the storage vacuoles of plants: a critical appraisal
    David G Robinson
    Heidelberg Institute for Plant Sciences, Cell Biology, University of Heidelberg, Im Neuenheimer Feld 230, D 69120 Heidelberg, Germany
    Traffic 6:615-25. 2005
    ..As a consequence, the plant Golgi apparatus is faced with the problem of recognizing proteins destined for lytic and storage vacuoles and segregating ..

Research Grants67

  1. Phosphorylation of FGF23 coordinates crosstalk between the skeleton and kidney
    VINCENT SCOTT TAGLIABRACCI; Fiscal Year: 2013
    ..as Fam20C, and showed that it belongs to a novel family of atypical protein kinases that localize within the Golgi apparatus and are secreted...
  2. Regulation of K-Ras by a Farnesyl-electrostatic Switch
    MARK REID PHILIPS; Fiscal Year: 2013
    ..the plasma membrane and association with intracellular membranes, including the endoplasmic reticulum (ER), Golgi apparatus and the outer mitochondrial membrane...
  3. Misregulation of receptor tyrosine kinase signaling in PKD
    Jordan A Kreidberg; Fiscal Year: 2013
    ..Failure to ubquitinate c-Met is due to sequestration of the E3 ubiquitin ligase c-Cbl by 31 integrin in the Golgi apparatus. This grant will investigate the roles of c-Cbl, c-Met and protein glycosylation in the pathogenesis of PKD.
  4. Distance-Dependent Structure and Function of Neuronal Dendrites
    Kristen M Harris; Fiscal Year: 2010
    ..that host excitory synapses, including microtubules for transport, polyribosomes for protein synthesis, Golgi apparatus for posttranslational modifications, endosomes for membrane recycling, and smooth endoplasmic reticulum (SER) ..
  5. Elevated Zinc in Ischemia and reperfusion
    Yang V Li; Fiscal Year: 2013
    ..and to study the Zn2+ release from intracellular storages such as mitochondria, endoplasmic reticulum, Golgi apparatus and lysosomes...
  6. Molecular Mechanisms of Host Function Exploitation by Type IV effectors of Legion
    Zhao Qing Luo; Fiscal Year: 2013
    ..that several yeast proteins involved in membrane transport between the endoplasmic reticulum and the cis-Golgi apparatus can suppress the toxicity of SdeA. More importantly, in a screening to search for L...
  7. Membrane Protein Trafficking in Photoreceptors
    Wolfgang Baehr; Fiscal Year: 2013
    ..Integral membrane proteins are synthesized by ER-associated ribosomes and exported to the Golgi apparatus. Prenylated or acylated peripheral membrane proteins are synthesized in the cytosol and become ER-associated...
  8. Second-hit and sexual dimorphism effects in PAH
    Pravin B Sehgal; Fiscal Year: 2013
    ..of PAH have an increase in cell size ("megalocytosis"), characteristic cytoplasmic organellar changes (Golgi apparatus fragmentation, cystic dilatation of the endoplasmic reticulum) and an inability to handle intracellular ..
  9. Intraflagellar Transport Proteins in Mice
    Gregory J Pazour; Fiscal Year: 2013
    ..We have shown that IFT20 is required for ciliary assembly and that it cycles between the Golgi apparatus and the cilium. At the Golgi membrane, IFT20 is complexed with the golgin protein GMAP210...
  10. Fluorescent Copper Probes for Intracellular Imaging
    Christoph J Fahrni; Fiscal Year: 2013
    ..Both imaging techniques will be applied to elucidate the role of the Golgi apparatus in copper redistribution and inheritance during mitosis and cell proliferation...
  11. STRUCTURAL BASIS OF ORGANELLE TETHERING
    Adam D Linstedt; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): The Golgi apparatus is compartmentalized into cisternae that associate with one another generating stacks...
  12. MICROTUBULE-BASED TRANSPORT IN LACRIMAL GLAND FUNCTION
    Sarah F Hamm-Alvarez; Fiscal Year: 2012
    ....
  13. Role of Mechanisms of Efflux in Manganese Homeostasis and Detoxification
    Somshuvra Mukhopadhyay; Fiscal Year: 2013
    ..We have now identified an important role for efflux of Mn via uptake into the Golgi apparatus followed by secretion in maintaining cellular Mn levels and protecting against excess Mn accumulation during ..
  14. Regulation of Alcohol-induced Fatty Liver Disease by Lipid Droplet Trafficking
    MARK A MC NIVEN; Fiscal Year: 2010
    ..The endoplasmic reticulum (ER), Golgi apparatus, and endosomal compartments have all been linked to lipid droplets...
  15. PtdIns 4-Kinase Regulation of Protein Sorting in the Golgi Apparatus
    Kathryn M Ferguson; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): The Golgi apparatus has two primary functions: biosynthesis of glycoproteins and glycolipids, and sorting...
  16. STRUCTURAL BASIS FOR SCAP/SREBP INTERACTION
    JOSHUA JAMES ZIAREK; Fiscal Year: 2013
    ..As sterol concentrations are reduced, SCAP escorts SREBP to the Golgi apparatus where it is activated...
  17. Mechanisms regulating PKGI proteolysis and modulation of pulmonary SMC phenotype
    Jesse D Roberts; Fiscal Year: 2013
    ..The role of the Golgi apparatus in PKGI proteolysis and of active nuclear transport in PKGI[unreadable] in nuclear PKGI signaling will be ..
  18. The Role of Arf GTPases in Endocytosis and Postendocytic Transport
    James E Casanova; Fiscal Year: 2010
    ..It is well established that Arfs promote the assembly of clathrin-coated vesicles in the Golgi apparatus and on endosomes, but their role in clathrin- mediated endocytosis at the plasma membrane is poorly ..
  19. INTEGRATED MODELING OF PROTEIN AND MEMBRANE TRAFFIC IN THE SECRETORY PATHWAY
    ROBERT PHAIR; Fiscal Year: 2013
    ..The endomembrane system itself consists of 3 major parts: the endoplasmic reticulum (ER), the Golgi apparatus and the plasma membrane, plus a complex series of internalization and recycling pathways grouped under the ..
  20. Structural Analysis of Golgi Trafficking Proteins
    Frederick M Hughson; Fiscal Year: 2013
    ..The Dsl1 complex functions in COPI vesicle transport from the Golgi apparatus to the endoplasmic reticulum (ER), a pathway essential for the recycling of the anterograde transport ..
  21. Functional mapping of SNARE-dependent trafficking and fusion
    Jeffrey E Pessin; Fiscal Year: 2013
    ..Thus, understanding the mechanism for this translocation process and the ability of insulin to increase glucose uptake are essentially process for the maintenance of normal glucose homeostasis. 1 ..
  22. Cyclic AMP Mediation of Epithelial Cell Function
    JAMES RICHARD GOLDENRING; Fiscal Year: 2012
    ..AKAP350 is localized to both centrosomes and the Golgi apparatus, and we have demonstrated that depletion of AKAP350A with siRNA leads to disruption of the Golgi structure as ..
  23. THE ROLE OF THE SECRETORY PATHWAY IN CRANIOFACIAL MORPHOGENESIS
    Ela W Knapik; Fiscal Year: 2010
    ..component of CORN vesicles, which take part in protein trafficking from the endoplasmic reticulum (ER) to the Golgi apparatus. A genetic lesion in the human SEC23A gene causes cranio-lenticulo-sutural dysplasia, which has similar ..
  24. Role of Rab Proteins in Golgi Apparatus Structure and Function
    Brian Storrie; Fiscal Year: 2013
    ..Characterization of structural/functional relationships within the mammalian Golgi apparatus and its regulatory pathways is important to human health...
  25. A novel vacuolar compartment in Toxoplasma gondii
    Silvia N Moreno; Fiscal Year: 2010
    ..This work is designed to test the hypothesis that a multivesicular endosome present in these parasites is involved in trafficking of proteins to acidocalcisomes, and has a role in osmoregulation and intracellular pH homeostasis. ..
  26. Compartment Specific Signaling of Ras
    MARK REID PHILIPS; Fiscal Year: 2010
    ..last cycle of GM55779 we discovered that N-Ras and H-Ras become activated in fibroblasts on both the PM and Golgi apparatus. We mapped the pathway to activation on Golgi as one involving EGFR/PLC(/DAG + Ca++/RasGRP1...
  27. Targeting and Function of Golgi Membrane Proteins
    Carolyn E Machamer; Fiscal Year: 2011
    ..The proposed experiments on golgin-160 will add to our understanding of cargo delivery to the cell surface and to the role of the Golgi complex in transducing stress signals to the rest of the cell. ..
  28. The Roles of Host Factors in the AMPylation Activity of a Bacterial Effector
    Zhao Qing Luo; Fiscal Year: 2012
    ..or more proteins involved in anterograde membrane transport between the endoplasmic reticulum (ER) and the Golgi apparatus. In the proposed study, we plan to: 1...
  29. A Small Molecule Approach Toward Understanding Bacterial Toxin Transport
    JOSE BERNARDO SAENZ; Fiscal Year: 2010
    ..evidence suggests that it could serve as a useful tool in elucidating ARF1-mediated transport through the Golgi apparatus. In addition, we hope to explore the role of kinases in toxin trafficking...
  30. Regulation of Arf GTPase activation at the trans-Golgi network
    J Christopher Fromme; Fiscal Year: 2013
    ..We expect that our research will lead to a clear model for how activation of Arf GTPases at the Golgi is regulated spatiotemporally. ..
  31. Epigenetic mechanisms and genes that regulate adipose tissue expansion
    Robert A Koza; Fiscal Year: 2013
    ..genes, mesoderm specific transcript (Mest);a maternally imprinted gene localized in the endoplasmic reticulum/Golgi apparatus where it may function as an epoxide hydrolase, lipase or acyltransferase based on homology with members of ..
  32. BIOGENESIS OF THE GOLGI APPARATUS
    Adam D Linstedt; Fiscal Year: 2013
    ....
  33. PHOSPHOLIPID DYNAMICS IN MEMBRANE ASSEMBLY
    Dennis R Voelker; Fiscal Year: 2012
    ..the genes and gene products involved in the export of phosphatidylethanolamine from mitochondria and the Golgi apparatus. In the fourth Specific Aim we will use recently developed conditional alleles for phosphatidylserine ..
  34. Mechanism of Targeting of a Luminal KDEL-containing Protein to the Golgi Complex
    DEBORAH ARONSON; Fiscal Year: 2010
    ..Our research will define new machinery that play a key role in promoting or preventing protein quality control. ..
  35. Cell cycle regulation of Golgi membrane dynamics
    Joachim Seemann; Fiscal Year: 2013
    ..In this application we propose to investigate the mechanisms that organize the division of the Golgi apparatus during mitosis...
  36. Toll-like receptor 9: trafficking and signaling
    Cynthia A Leifer; Fiscal Year: 2013
    ..This knowledge will provide the basis for the further development of CpG DNA as an adjuvant and therapeutic as well as develop mechanisms to interrupt the cycle of autoimmune pathology. ..
  37. The role of Rap1 and its novel associated molecules in T cell responses
    Vassiliki A Boussiotis; Fiscal Year: 2011
    ..In T cells, TCR and CD28- mediated Ras activation occurs at the Golgi apparatus and requires phospholipase Cγ, whereas LFA-1- mediated Ras activation occurs at the plasma membrane ..
  38. Mechanisms of HTLV-I Tax-mediated NF-kB activation
    Edward W Harhaj; Fiscal Year: 2013
    ..Tax activation of NF-kB correlates with ubiquitin-dependent relocalization of IKK to the Golgi apparatus. Tax is polyubiquitinated via Lys 63-linked ubiquitin chains and requires the ubiquitin conjugating enzyme ..
  39. Role of Phospholipid Remodeling in Secretion and Golgi Function in Mammalian Cell
    William J Brown; Fiscal Year: 2013
    ..These studies will reveal novel biological roles for PLA and LPAT enzymes in mediating intracellular trafficking events in the secretory pathway and advance our understanding of membrane tubule-mediated transport. ..
  40. Regulation of Mitochondrial Release from Microtubules during Cell Division
    Jarom Y Chung; Fiscal Year: 2013
    ..However, proper cellular function also requires the partitioning of organelles. The mechanism behind ER, Golgi apparatus, and peroxisomes inheritance among others are better understood, but to date, that of mitochondrial ..
  41. Role of Retromer-mediated Retrograde Transport in HPV Entry
    DANIEL C DIMAIO; Fiscal Year: 2013
    ..numerous novel essential factors, including several factors involved in retrograde transport to the Golgi apparatus. Prominent validated hits encode components of the retromer, a molecular machine involved in transport of ..
  42. Biogenesis and operation of the human cytomegalovirus assembly complex
    Philip E Pellett; Fiscal Year: 2012
    ..set of nested cylinders, with the outer cylinder consisting of networks of tubular vesicles derived from the Golgi apparatus and the trans-Golgi network;the inner cylinder consists of vesicles derived from recycling endosomes where ..
  43. Using Drosophila Neurons to Identify Mechanisms that Control Microtubule Polarity
    Melissa Rolls; Fiscal Year: 2013
    ..Our results will form a foundation for understanding neurological diseases ranging from motor neuron disease to Williams syndrome, as they result from perturbations in microtubule organization or trafficking. ..
  44. Ubiquitin and Cell Cycle Regulation of Golgi Membrane Dynamics
    Yanzhuang Wang; Fiscal Year: 2013
    ..during mitosis, which are partitioned into the daughter cells where they are reassembled into a new Golgi apparatus. Reassembly is mediated by two ATPases (NSF and p97) that fuse the membranes...
  45. THE TRANSITIONAL ER-GOLGI SYSTEM IN BUDDING YEASTS
    Benjamin S Glick; Fiscal Year: 2012
    ..This project focuses on the transitional ER (tER) and the Golgi apparatus. tER sites are membrane domains that produce COPII transport vesicles...
  46. Schizophrenia as a Disorder of Glutamate Receptor Trafficking
    James H Meador-Woodruff; Fiscal Year: 2013
    ..past 5 years support this model, with evidence for accelerated exit from the endoplasmic reticulum (ER) and Golgi apparatus of AMPA and NMDAR complexes including changes in N-linked glycosylation and expression of ER exit and ..
  47. Spatial Regulation of Cytoskeletal Asymmetry
    Irina Kaverina; Fiscal Year: 2012
    ..We have recently demonstrated that a large number of microtubules originate from the Golgi apparatus (Efimov et al, 2007)...
  48. Sorting and trafficking in the endosomal system
    Christopher G Burd; Fiscal Year: 2013
    ..lipids in the endosome are packaged into transport carriers that bud from the endosome and ferry cargo to the Golgi apparatus and other organelles...
  49. Mechanisms of COPII-dependent Transport
    CHARLES K BARLOWE; Fiscal Year: 2013
    ..Therefore these studies are basic for understanding numerous health related issues including cholesterol regulation, cystic fibrosis and diabetes. ..
  50. ROLE AND MECHANISMS OF EFFLUX IN MANGANESE HOMEOSTASIS AND DETOXIFICATION
    Somshuvra Mukhopadhyay; Fiscal Year: 2013
    ..The investigators have now identified an important role for efflux of Mn via uptake into the Golgi apparatus followed by secretion in maintaining cellular Mn levels and protecting against excess Mn accumulation during ..
  51. The Role of Ceramidases in Cancer Chemotherapy
    Cungui Mao; Fiscal Year: 2013
    ..These studies will provide insights into the role and mechanism of action of ACER2 and its product SPH in mediating anti-proliferative responses in cancer cells. ..
  52. Subcellular Mechanisms in Pathogenesis of Pulmonary Arterial Hypertension
    JASON EDWARD LEE; Fiscal Year: 2012
    ..to the wrong subcellular compartments due to defects in intracellular trafficking at the level of the Golgi apparatus ("Golgi blockade hypothesis")...
  53. Structure and Function of Myosin VI
    H Lee Sweeney; Fiscal Year: 2013
    ..it also can function as a strain-dependent anchor that is involved in organizing structures such as the Golgi apparatus. Myosin VI is found as a monomer in cells, but functions optimally as a dimer...
  54. Characterization of mammalian COG complex-interacting intra-Golgi trafficking mac
    Vladimir V Lupashin; Fiscal Year: 2012
    ..analysis of the machinery that determines high-fidelity bidirectional protein and lipid trafficking in the Golgi apparatus. The functional interplay between SNAREs, Rabs and tethering factors will be investigated;we also hope to ..
  55. Live-Cell Assays of ER and Golgi Enzyme Activities
    JOHN J NALEWAY; Fiscal Year: 2013
    ..I project aims to develop new targeted fluorogenic substrates capable of measuring enzyme activities in the Golgi apparatus and Endoplasmic Reticulum (ER) of living cells and tissues...
  56. Intercellular Signaling and Endosome to Golgi Transport in Multicellular Animals
    Richard W Padgett; Fiscal Year: 2013
    ..and understand the cellular components that transport internalized endocytic cargo from endosomes to the Golgi apparatus (retrograde recycling), and to understand how this process regulates, and is integrated within, intercellular ..
  57. Three dimensional aberration corrected scanning transmission electron microscopy
    David W Piston; Fiscal Year: 2011
    ..g., ribosomes, proteasomes, Golgi apparatus, and mitochondria) within Eukaryotic cells...
  58. Super-Res Holographic Microscopy to Advance Research on Golgi Apparatus Function
    Brian Storrie; Fiscal Year: 2013
    ..3. At Ben Gurion University (BGU), Rosen will continue to develop advanced algorithms and concepts based upon FINCH to improve performance and resolving power. ..
  59. Structural Studies of Lysosomal Storage Diseases
    Scott C Garman; Fiscal Year: 2013
    ..where it must fold correctly;it must be post-translationally modified, allowing it to traffi through the Golgi apparatus to the lysosome;there, it must have the correct catalytic machinery and the stability to perform its ..
  60. ON THE ROLE OF THE HPS GENE PRODUCT IN MELANOCYTES
    Raymond Boissy; Fiscal Year: 2002
    ..In common, these organelles either originate and/or receive specific glycoproteins from the Golgi apparatus. Thus it is hypothesized that the HPS gene product regulates selective trafficking from the trans Golgi ..
  61. ASSEMBLY OF NASCENT PLASMA LIPOPROTEINS BY HEPATOCYTES
    Robert Hamilton; Fiscal Year: 1991
    ..The first is to thoroughly characterize nascent very low density lipoproteins (VLDL) isolated from the Golgi apparatus. The second is to determine the mechanism(s) by which nascent VLDL contribute to the formation of plasma high ..
  62. Mechanism of centrosome regulation from the Golgi
    Christine Suetterlin; Fiscal Year: 2013
    ..of this project is to understand a control mechanism that regulates the centrosome from the adjacent Golgi apparatus in mammalian cells...
  63. Amyloid Beta-Protein: ApoE and Cholesterol Homeostasis
    WELLINGTON WOOD; Fiscal Year: 2009
    ..Determine if the neuronal apoE4 and apoE-null phenotype can be "rescued" by incubation with astrocytes of apoE2, apoE3, or wildtype mice. In some experiments astrocytes will be treated with ABeta1-42. ..
  64. PENETRATION OF MACROMOLEULCES INTO CELLS
    H Ryser; Fiscal Year: 1999
    ..by intracellular cleavage which, according to our preliminary data on cell fractionation, occurs in the Golgi apparatus. Our experimental approach will be: 1) to clarify the functional significance of the surface cleavage; 2) to ..
  65. GLYCOSYLTRANSFERASE FUNCTION DURING FERTILIZATION
    BARRY SHUR; Fiscal Year: 2006
    ..Finally, in the third Aim, we will continue our analysis of GalT-mediated signal transduction leading to the acrosome reaction. ..
  66. ENDOMEMBRANE TRAFFICKING OF FIV ENVELOPE GLYCOPROTEINS
    D Morre; Fiscal Year: 1993
    ..proposal is to characterize the endomembrane trafficking between transitional endoplasmic reticulum and cis Golgi apparatus in lentivirus coat protein processing as a strategy for target identification in antiviral drug design using ..
  67. GLYCOSYLATION MECHANISMS
    CARLOS HIRSCHBERG; Fiscal Year: 2000
    ..mechanisms which regulate the glycosylation of proteins and lipids in the rough endoplasmic reticulum and Golgi apparatus and, (b) to establish the role of protein and lipid glycosylation in organelle topography and ..