Genomes and Genes
Summary: A stack of flattened vesicles that functions in posttranslational processing and sorting of proteins, receiving them from the rough ENDOPLASMIC RETICULUM and directing them to secretory vesicles, LYSOSOMES, or the CELL MEMBRANE. The movement of proteins takes place by transfer vesicles that bud off from the rough endoplasmic reticulum or Golgi apparatus and fuse with the Golgi, lysosomes or cell membrane. (From Glick, Glossary of Biochemistry and Molecular Biology, 1990)
Publications225 found, 100 shown here
- A comprehensive comparison of transmembrane domains reveals organelle-specific propertiesHayley J Sharpe
MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, UK
Cell 142:158-69. 2010..The pervasive presence of organelle-specific features among the TMDs of a particular organelle has implications for TMD prediction, regulation of protein activity by location, and sorting of proteins and lipids in the secretory pathway...
- NKG2D ligand MICA is retained in the cis-Golgi apparatus by human cytomegalovirus protein UL142Omodele Ashiru
Department of Pathology, Cambridge Institute for Medical Research, University of Cambridge, Cambridge, United Kingdom
J Virol 83:12345-54. 2009..Here, we show that UL142 localizes predominantly to the endoplasmic reticulum (ER) and cis-Golgi apparatus. The transmembrane domain of UL142 mediates its ER localization, while we propose that the UL142 luminal ..
- Adult-onset primary open-angle glaucoma caused by mutations in optineurinTayebeh Rezaie
Molecular Ophthalmic Genetics Laboratory, Surgical Research Center, Department of Surgery, University of Connecticut Health Center, Farmington, CT 06030, USA
Science 295:1077-9. 2002..Optineurin is expressed in trabecular meshwork, nonpigmented ciliary epithelium, retina, and brain, and we speculate that it plays a neuroprotective role...
- Phosphoinositides in cell regulation and membrane dynamicsGilbert Di Paolo
Department of Pathology and Cell Biology, The Taub Institute for Research on Alzheimer s Disease and the Aging Brain, Columbia University Medical Center, New York, New York 10032, USA
Nature 443:651-7. 2006..Their functions, besides classical signal transduction at the cell surface, include regulation of membrane traffic, the cytoskeleton, nuclear events and the permeability and transport functions of membranes...
- Coatomer-dependent protein delivery to lipid dropletsKrishnakant G Soni
Cell Biology and Metabolism Program, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
J Cell Sci 122:1834-41. 2009..Here we demonstrate that ATGL and ADRP, but not TIP47, are delivered to LDs by a pathway mediated by the COPI and COPII coatomer proteins and their corresponding regulators...
- PTRF-Cavin, a conserved cytoplasmic protein required for caveola formation and functionMichelle M Hill
Institute for Molecular Bioscience, University of Queensland, Brisbane, Queensland 4072, Australia
Cell 132:113-24. 2008..We conclude that PTRF-Cavin is required for caveola formation and sequestration of mobile caveolin into immobile caveolae...
- Intracellular functions of N-linked glycansA Helenius
Institute of Biochemistry, , , , Switzerland
Science 291:2364-9. 2001..The division of synthesis and processing between the ER and the Golgi complex represents an evolutionary adaptation that allows efficient exploitation of the potential of oligosaccharides...
- Membrane lipids: where they are and how they behaveGerrit van Meer
Bijvoet Center and Institute of Biomembranes, Utrecht University, 3584 CH Utrecht, The Netherlands
Nat Rev Mol Cell Biol 9:112-24. 2008..enzymes, translocases and transporters, plus the intrinsic physical phase behaviour of lipids and their interactions with membrane proteins, to create the unique compositions and multiple functionalities of their individual membranes?..
- Systematic analysis of SNARE molecules in Arabidopsis: dissection of the post-Golgi network in plant cellsTomohiro Uemura
Graduate School of Biostudies, Kyoto University, Yoshida-nihonmatsu, Sakyo-ku, Kyoto 606-8501, Japan
Cell Struct Funct 29:49-65. 2004..SNARE proteins were located on specific intracellular compartments: 6 in the endoplasmic reticulum, 9 in the Golgi apparatus, 4 in the trans-Golgi network (TGN), 2 in endosomes, 17 on the plasma membrane, 7 in both the prevacuolar ..
- Mutations in dynein link motor neuron degeneration to defects in retrograde transportMajid Hafezparast
Department of Neurodegenerative Disease, Institute of Neurology, National Hospital for Neurology and Neurosurgery, Queen Square, London WC1N 3BG, UK
Science 300:808-12. 2003..These mutations exclusively perturb neuron-specific functions of dynein...
- Coordinated lipid transfer between the endoplasmic reticulum and the Golgi complex requires the VAP proteins and is essential for Golgi-mediated transportDiego Peretti
The Molecular Cell Biology Department, Weizmann Institute of Science, Rehovot 76100, Israel
Mol Biol Cell 19:3871-84. 2008..We propose that Nir2, OSBP, and CERT function coordinately at the ER-Golgi membrane contact sites, thereby affecting the lipid composition of the Golgi membranes and consequently their structural and functional identities...
- Multiple cytosolic and transmembrane determinants are required for the trafficking of SCAMP1 via an ER-Golgi-TGN-PM pathwayYi Cai
School of Life Sciences, Centre for Cell and Developmental Biology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China
Plant J 65:882-96. 2011..Therefore, both the cytosolic N-terminus and TMD sequences of SCAMP1 play integral roles in mediating its transport to the PM via an ER-Golgi-TGN pathway...
- Subcompartment localization of the side chain xyloglucan-synthesizing enzymes within Golgi stacks of tobacco suspension-cultured cellsLaurence Chevalier
Laboratoire Glycobiologie et Matrice Extracellulaire Végétale, UPRES EA 4358, Institut Federatif de Recherche Multidisciplinaire sur les Peptides 23, Plate forme de Recherche en Imagerie Cellulaire de Haute Normandie PRIMACEN, IBiSA, Universite de Rouen, 76821 Mont Saint Aignan Cedex, France
Plant J 64:977-89. 2010..that: (i) all fusion proteins, AtXT1-GFP, AtMUR3-GFP and AtFUT1-GFP are specifically targeted to the Golgi apparatus; and (ii) AtXT1-GFP is mainly located in the cis and medial cisternae, AtMUR3-GFP is predominantly associated ..
- Functional interplay between LIS1, NDE1 and NDEL1 in dynein-dependent organelle positioningConnie Lam
Faculty of Life Sciences, University of Manchester, Manchester, M13 9PT, UK
J Cell Sci 123:202-12. 2010..Consistent with this hypothesis, expression of exogenous NDE1 or NDEL1 rescued the effects of LIS1 depletion on Golgi organisation, whereas LIS1 was only partially effective at rescuing the loss of NDE1 and NDEL1...
- Loss of Apm1, the micro1 subunit of the clathrin-associated adaptor-protein-1 complex, causes distinct phenotypes and synthetic lethality with calcineurin deletion in fission yeastAyako Kita
Division of Molecular Pharmacology and Pharmacogenomics, Department of Genome Sciences, Kobe University Graduate School of Medicine, Kobe 650 0017, Japan
Mol Biol Cell 15:2920-31. 2004....
- Identification of a xylogalacturonan xylosyltransferase involved in pectin biosynthesis in ArabidopsisJacob Krüger Jensen
Laboratory of Molecular Plant Biology, Department of Plant Biology, Faculty of Life Sciences, University of Copenhagen, DK 1871 Frederiksberg C, Denmark
Plant Cell 20:1289-302. 2008..The protein was shown by expression of a fluorescent fusion protein in N. benthamiana to be localized in the Golgi vesicles as expected for a glycosyltransferase involved in pectin biosynthesis...
- The rice alpha-amylase glycoprotein is targeted from the Golgi apparatus through the secretory pathway to the plastidsAya Kitajima
Graduate School of Science and Technology, Niigata University, Niigata, Japan
Plant Cell 21:2844-58. 2009..Thus, Golgi-to-plastid traffic appears to be involved in the transport of glycoproteins to plastids and plastid targeting seems to be accomplished in a sorting signal-dependent manner...
- Salt tolerance of Arabidopsis thaliana requires maturation of N-glycosylated proteins in the Golgi apparatusJae Sook Kang
Brain Korea 21, Division of Applied Life Science and Environmental Biotechnology National Core Research Center, Graduate School of Gyeongsang National University, Jinju 660 701, Korea
Proc Natl Acad Sci U S A 105:5933-8. 2008Protein N-glycosylation in the endoplasmic reticulum (ER) and in the Golgi apparatus is an essential process in eukaryotic cells...
- A myosin XI tail domain homologous to the yeast myosin vacuole-binding domain interacts with plastids and stromules in Nicotiana benthamianaAmir Sattarzadeh
Department of Molecular Biology and Genetics, Biotechnology Building, Cornell University, Ithaca, NY 14853, USA
Mol Plant 2:1351-8. 2009..benthamiana. Taken together, these observations implicate myosin motor proteins in dynamics of plastids and stromules...
- Protein sorting to the storage vacuoles of plants: a critical appraisalDavid G Robinson
Heidelberg Institute for Plant Sciences, Cell Biology, University of Heidelberg, Im Neuenheimer Feld 230, D-69120 Heidelberg, Germany
Traffic 6:615-25. 2005..As a consequence, the plant Golgi apparatus is faced with the problem of recognizing proteins destined for lytic and storage vacuoles and segregating ..
- The destination for single-pass membrane proteins is influenced markedly by the length of the hydrophobic domainFederica Brandizzi
Research School of Biological and Molecular Sciences, Oxford Brookes University, Gipsy Lane Campus, OX3 0BP Headington-Oxford, United Kingdom
Plant Cell 14:1077-92. 2002..of the TM20-labeled compartments was investigated by coexpression with a marker localized mainly in the Golgi apparatus, AtERD2, fused to a yellow fluorescent protein...
- Distinct AAA-ATPase p97 complexes function in discrete steps of nuclear assemblyM Hetzer
European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
Nat Cell Biol 3:1086-91. 2001..Subsequent NE growth involves a p97-p47 complex. This study provides the first insights into the molecular mechanisms and specificity of fusion events involved in NE formation...
- Identification of a family of animal sphingomyelin synthasesKlazien Huitema
Department of Membrane Enzymology, Faculty of Chemistry, Institute of Biomembranes, Utrecht University, Utrecht, The Netherlands
EMBO J 23:33-44. 2004..Whereas human SMS1 is localised to the Golgi, SMS2 resides primarily at the plasma membrane. Collectively, these findings open up important new avenues for studying sphingolipid function in animals...
- Chlamydia causes fragmentation of the Golgi compartment to ensure reproductionDagmar Heuer
Department of Molecular Biology, Max Planck Institute for Infection Biology, Chariteplatz 1, 10117 Berlin, Germany
Nature 457:731-5. 2009..for host cell lipids, such as sphingolipids and cholesterol, produced in the endoplasmic reticulum and the Golgi apparatus. However, the mechanisms by which intracellular pathogens acquire host cell lipids are not well understood...
- Signals for sorting of transmembrane proteins to endosomes and lysosomesJuan S Bonifacino
Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
Annu Rev Biochem 72:395-447. 2003..This complex array of signals and recognition proteins ensures the dynamic but accurate distribution of transmembrane proteins to different compartments of the endosomal-lysosomal system...
- Asymmetric CLASP-dependent nucleation of noncentrosomal microtubules at the trans-Golgi networkAndrey Efimov
Department of Cell and Developmental Biology, Vanderbilt University Medical Center, Nashville, TN 37232, USA
Dev Cell 12:917-30. 2007..Here we demonstrate that a large number of microtubules in untreated human cells originate from the Golgi apparatus in a centrosome-independent manner...
- Class 1 reversibly glycosylated polypeptides are plasmodesmal-associated proteins delivered to plasmodesmata via the golgi apparatusGuy Sagi
Department of Plant Sciences, George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel
Plant Cell 17:1788-800. 2005..Localization to the Golgi apparatus was verified by colocalization of transiently expressed AtRGP2 fused to cyan fluorescence protein together ..
- Molecular cloning and characterization of Arabidopsis thaliana Golgi alpha-mannosidase II, a key enzyme in the formation of complex N-glycans in plantsRichard Strasser
Institute of Applied Genetics and Cell Biology, Vienna, Austria
Plant J 45:789-803. 2006..domain and 10 amino acids from the cytoplasmic tail are sufficient to retain a reporter molecule in the Golgi apparatus and that lumenal sequences are not involved in the retention mechanism...
- Dissection of Arabidopsis ADP-RIBOSYLATION FACTOR 1 function in epidermal cell polarityJian Xu
Department of Molecular Cell Biology, Utrecht University, 3584 CH Utrecht, The Netherlands
Plant Cell 17:525-36. 2005..Fluorescence colocalization studies reveal that ARF1 is localized to the Golgi apparatus and endocytic organelles in both onion (Allium cepa) and Arabidopsis cells...
- Golgi transporters: opening the gate to cell wall polysaccharide biosynthesisFrancisca Reyes
The Millennium Nucleus in Plant Cell Biotechnology, Fundación Ciencia para la Vida, Center of Plant Biotechnology, Andres Bello University, Republica 217, Santiago, Chile
Curr Opin Plant Biol 11:244-51. 2008The synthesis of non-cellulosic polysaccharides occurs in the Golgi apparatus and requires a great number of metabolites (substrates and ions)...
- Exiting the Golgi complexMaria Antonietta De Matteis
Department of Cell Biology and Oncology, Consorzio Mario Negri Sud, 66030 Santa Maria Imbaro CH, Italy
Nat Rev Mol Cell Biol 9:273-84. 2008....
- Nanoscale architecture of endoplasmic reticulum export sites and of Golgi membranes as determined by electron tomographyL Andrew Staehelin
Molecular Cellular and Developmental Biology, University of Colorado, Boulder, Colorado 80309, USA
Plant Physiol 147:1454-68. 2008
- The ZIP7 gene (Slc39a7) encodes a zinc transporter involved in zinc homeostasis of the Golgi apparatusLiping Huang
Agricultural Research Service, United States Department of Agriculture, Western Human Nutrition Research Center, University of California at Davis, 95616, USA
J Biol Chem 280:15456-63. 2005..The endogenous ZIP7 was associated with the Golgi apparatus and was capable of transporting zinc from the Golgi apparatus into the cytoplasm of the cell...
- Assembly and maturation of the flavivirus Kunjin virus appear to occur in the rough endoplasmic reticulum and along the secretory pathway, respectivelyJ M MacKenzie
Clinical Medical Virology Centre, University of Queensland, St Lucia, Queensland 4072, Australia
J Virol 75:10787-99. 2001..virions from the RER involves the transport of individual virions within individual vesicles en route to the Golgi apparatus. The results suggest that the assembly of virions occurs within the lumen of the RER and that subsequent ..
- Reevaluation of the effects of brefeldin A on plant cells using tobacco Bright Yellow 2 cells expressing Golgi-targeted green fluorescent protein and COPI antiseraChristophe Ritzenthaler
Institut de Biologie Moleculaire des Plantes, 67084 Strasbourg Cedex, France
Plant Cell 14:237-61. 2002..A (BFA) causes a block in the secretory system of eukaryotic cells by inhibiting vesicle formation at the Golgi apparatus. Although this toxin has been used in many studies, its effects on plant cells are still shrouded in ..
- Movement and remodeling of the endoplasmic reticulum in nondividing cells of tobacco leavesI Sparkes
School of Life Sciences, Oxford Brookes University, Oxford OX3 0BP, United Kingdom
Plant Cell 21:3937-49. 2009....
- Association of the cellular coactivator HCF-1 with the Golgi apparatus in sensory neuronsGaelle Kolb
National Institutes of Health, 4 129, 4 Center Drive, Bethesda, MD 20892, USA
J Virol 82:9555-63. 2008..Using a primary neuronal cell culture system, HCF-1 was localized to the Golgi apparatus in unstimulated neurons, a unique location for a transcriptional coactivator...
- Direct binding of ubiquitin conjugates by the mammalian p97 adaptor complexes, p47 and Ufd1-Npl4Hemmo H Meyer
Department of Cell Biology, Yale University School of Medicine, 333 Cedar Street, SHM, C441, PO Box 208002, New Haven, CT 06520 8002, USA
EMBO J 21:5645-52. 2002..In the case of p47, which is involved in the reassembly of the ER, the nuclear envelope and the Golgi apparatus, binding is mediated by a UBA domain...
- Sequential interactions with Sec23 control the direction of vesicle trafficChristopher Lord
Department of Cellular and Molecular Medicine, Howard Hughes Medical Institute, University of California at San Diego, La Jolla, California 92093 0668, USA
Nature 473:181-6. 2011..These events are conserved in mammalian cells...
- Rabs and their effectors: achieving specificity in membrane trafficBianka L Grosshans
Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06520, USA
Proc Natl Acad Sci U S A 103:11821-7. 2006..Additionally, Rab cascades and Rab conversions appear to confer directionality to membrane traffic and couple each stage of traffic with the next along the pathway...
- Arabidopsis thaliana RGXT1 and RGXT2 encode Golgi-localized (1,3)-alpha-D-xylosyltransferases involved in the synthesis of pectic rhamnogalacturonan-IIJack Egelund
Biotechnology Group, Danish Institute of Agricultural Sciences and Center for Molecular Plant Physiology, DK 1871 Frederiksberg C, Denmark
Plant Cell 18:2593-607. 2006..and also colocalized with the Golgi marker dye BODIPY TR ceramide, consistent with targeting to the Golgi apparatus. Taken together, these results suggest that RGXT1 and RGXT2 encode Golgi-localized (1,3)-alpha-d-..
- Alpha-synuclein-induced aggregation of cytoplasmic vesicles in Saccharomyces cerevisiaeJames H Soper
Center for Neurodegenerative Disease Research and Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
Mol Biol Cell 19:1093-103. 2008....
- Regulation of dynactin through the differential expression of p150Glued isoformsRam Dixit
Department of Physiology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
J Biol Chem 283:33611-9. 2008..Together, these studies provide further insight into the regulation of dynein-dynactin function in the cell...
- Lipids and cholesterol as regulators of traffic in the endomembrane systemJennifer Lippincott-Schwartz
Cell Biology and Metabolism Program, NICHD, NIH, Bethesda, Maryland, USA
Annu Rev Biophys 39:559-78. 2010..pathways-biosynthetic and endocytic, each with specific lipid components-make up this system, with the Golgi apparatus mediating transport between the two...
- MAPK signaling to the early secretory pathway revealed by kinase/phosphatase functional screeningHesso Farhan
Biozentrum, Universitat Basel, 4056 Basel, Switzerland
J Cell Biol 189:997-1011. 2010..122 kinases/phosphatases that affect endoplasmic reticulum (ER) export, ER exit sites (ERESs), and/or the Golgi apparatus. Numerous kinases/phosphatases regulate the number of ERESs and ER to Golgi protein trafficking...
- The Golgi apparatus is a primary site of intracellular damage after photosensitization with Rose Bengal acetateC Soldani
Dipartimento di Biologia Animale, , Piazza Botta 10, 27100 Pavia, Italy
Eur J Histochem 48:443-8. 2004The aim of the present investigation was to elucidate whether the Golgi apparatus undergoes photodamage following administration of the fluorogenic substrates Rose Bengal acetate (RBAc) and irradiation at the appropriate wavelength...
- PtdIns4P recognition by Vps74/GOLPH3 links PtdIns 4-kinase signaling to retrograde Golgi traffickingChristopher S Wood
Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, 19104, USA
J Cell Biol 187:967-75. 2009Targeting and retention of resident integral membrane proteins of the Golgi apparatus underly the function of the Golgi in glycoprotein and glycolipid processing and sorting...
- A genome-wide RNA interference screen identifies two novel components of the metazoan secretory pathwayFranz Wendler
MRC National Institute for Medical Research, London, UK
EMBO J 29:304-14. 2010..Given this coverage, the small number of novel components suggests that contrary to previous indications the number of essential core components of the secretory pathway is not much greater in metazoans than in yeasts...
- A missense mutation in the Arabidopsis COPII coat protein Sec24A induces the formation of clusters of the endoplasmic reticulum and Golgi apparatusCarmen Faso
Department of Energy, Plant Research Laboratory, Michigan State University, East Lansing, MI 48824, USA
Plant Cell 21:3655-71. 2009How the endoplasmic reticulum (ER) and the Golgi apparatus maintain their morphological and functional identity while working in concert to ensure the production of biomolecules necessary for the cell's survival is a fundamental question ..
- Golgi apparatus fragmentation as a mechanism responsible for uniform delivery of uroplakins to the apical plasma membrane of uroepithelial cellsMateja Erdani Kreft
Institute of Cell Biology, Faculty of Medicine, University of Ljubljana, SI 1000 Ljubljana, Slovenia
Biol Cell 102:593-607. 2010The GA (Golgi apparatus) has an essential role in membrane trafficking, determining the assembly and delivery of UPs (uroplakins) to the APM (apical plasma membrane) of superficial UCs (uroepithelial cells) of urinary bladder...
- How the Golgi works: a cisternal progenitor modelSuzanne R Pfeffer
Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305 5307, USA
Proc Natl Acad Sci U S A 107:19614-8. 2010....
- Death receptor ligation triggers membrane scrambling between Golgi and mitochondriaS Ouasti
Faculty of Life Sciences, The University of Manchester, Stopford Building, Oxford Road, Manchester, UK
Cell Death Differ 14:453-61. 2007..Thus, our work unveils that Fas ligand-mediated apoptosis induces scrambling of mitochondrial and secretory organelles via a global alteration of membrane traffic that is modulated by apical caspases...
- Loss of the Batten disease gene CLN3 prevents exit from the TGN of the mannose 6-phosphate receptorDaniel J Metcalf
MRC Cell Biology Unit, MRC Laboratory for Molecular Cell Biology and Department of Cell and Developmental Biology, University College London, Gower Street, London WC1E6BT, UK
Traffic 9:1905-14. 2008..CLN3 is therefore essential for trafficking along the route needed for delivery of lysosomal enzymes, and its loss thereby contributes to and may explain the lysosomal dysfunction underlying Batten disease...
- SAP97 and CASK mediate sorting of NMDA receptors through a previously unknown secretory pathwayOkunola Jeyifous
Department of Psychiatry and Behavioral Science, Stanford University, Palo Alto, California, USA
Nat Neurosci 12:1011-9. 2009..These findings indicate that NMDARs are sorted away from AMPARs via a non-conventional secretory pathway that utilizes dendritic Golgi outposts...
- The hydrophobic domain of infectious bronchitis virus E protein alters the host secretory pathway and is important for release of infectious virusTravis R Ruch
Department of Cell Biology, The Johns Hopkins University School of Medicine, 725 N Wolfe St, Baltimore, MD 21205, USA
J Virol 85:675-85. 2011..Our results indicated that the hydrophobic domain of IBV E alters the host secretory pathway to the apparent advantage of the virus...
- Hypo-phosphorylation of salivary peptidome as a clue to the molecular pathogenesis of autism spectrum disordersMassimo Castagnola
Istituto di Biochimica e Biochimica Clinica, Facolta di Medicina, Universita Cattolica, Rome, Italy
J Proteome Res 7:5327-32. 2008..These results suggest that analysis of salivary phospho-peptides might help to discriminate a considerable subgroup of ASD patients...
- Alpha-synuclein delays endoplasmic reticulum (ER)-to-Golgi transport in mammalian cells by antagonizing ER/Golgi SNAREsNandhakumar Thayanidhi
University of Montana, Missoula, MT 59812 4824, USA
Mol Biol Cell 21:1850-63. 2010..Finally, soluble alpha-synuclein A53T directly bound ER/Golgi SNAREs and inhibited SNARE complex assembly, providing a potential mechanism for toxic effects in the early secretory pathway...
- Sequential depletion and acquisition of proteins during Golgi stack disassembly and reformationJennifer Schoberer
Department of Applied Genetics and Cell Biology, University of Natural Resources and Applied Life Sciences, Vienna, Muthgasse 18, 1190 Vienna, Austria
Traffic 11:1429-44. 2010Herein, we report the stepwise transport of multiple plant Golgi membrane markers during disassembly of the Golgi apparatus in tobacco leaf epidermal cells in response to the induced expression of the GTP-locked Sar1p or Brefeldin A (BFA)..
- Yeast ARV1 is required for efficient delivery of an early GPI intermediate to the first mannosyltransferase during GPI assembly and controls lipid flow from the endoplasmic reticulumKentaro Kajiwara
Department of Bioresource Science and Technology, Graduate School of Biosphere Science, Hiroshima University, Higashi Hiroshima 739 8528, Japan
Mol Biol Cell 19:2069-82. 2008....
- Microtubule network asymmetry in motile cells: role of Golgi-derived arrayTatiana Vinogradova
Department of Cell and Developmental Biology, Vanderbilt University Medical Center, Nashville, TN, USA
Cell Cycle 8:2168-74. 2009..Importantly, an asymmetric MT array is formed at a non-conventional MT nucleation site, the Golgi. Here, we emphasize the contribution of this array to the asymmetry of MT network...
- Cdc42 and noncanonical Wnt signal transduction pathways cooperate to promote cell polarityKarni Schlessinger
Medical Research Council Laboratory for Molecular Cell Biology, Cancer Research UK Oncogene and Signal Transduction Group and Department of Biochemistry and Molecular Biology, University College London, London, England, UK
J Cell Biol 178:355-61. 2007....
- Nitric oxide synthase generates nitric oxide locally to regulate compartmentalized protein S-nitrosylation and protein traffickingYasuko Iwakiri
Section of Digestive Diseases, Department of Pharmacology, Yale University School of Medicine, New Haven, CT 06510, USA
Proc Natl Acad Sci U S A 103:19777-82. 2006..Endothelial NOS (eNOS) is localized mainly on the Golgi apparatus and in plasma membrane caveolae...
- ICA69 is a novel Rab2 effector regulating ER-Golgi trafficking in insulinoma cellsLaura Buffa
Experimental Diabetology, Carl Gustav Carus School of Medicine, Dresden University of Technology, D 01307 Dresden, Germany
Eur J Cell Biol 87:197-209. 2008..Taken together, these data identify ICA69 as a novel Rab2 effector and point to its role in regulating the early transport of insulin secretory granule proteins...
- Diacidic motifs influence the export of transmembrane proteins from the endoplasmic reticulum in plant cellsSally L Hanton
Department of Biology, University of Saskatchewan, Saskatoon SK S7N 5E2, Saskatchewan, Canada
Plant Cell 17:3081-93. 2005..We show that diacidic motifs perform a significant function in the export of transmembrane proteins to the Golgi apparatus, as mutations of these signals impede the efficient anterograde transport of multispanning, type II, and type ..
- HTLV-1 Tax is a critical lipid raft modulator that hijacks IkappaB kinases to the microdomains for persistent activation of NF-kappaBJiannan Huang
Penn State Cancer Institute, Department of Medicine, Penn State University College of Medicine, Hershey, Pennsylvania 17033, USA
J Biol Chem 284:6208-17. 2009..These findings indicate that Tax1 actively recruits IKK to the lipid raft microdomains for persistent activation of NF-kappaB, thereby contributing to HTLV-1 oncogenesis...
- The Golgi apparatus at the cell centreRosa M Rios
Departamento de Microbiologia, Facultad de Biologia, Universidad de Sevilla, Reina Mercedes 6, 41012 Sevilla, Spain
Curr Opin Cell Biol 15:60-6. 2003In non-polarised mammalian cells, the Golgi apparatus is localised around the centrosome and actively maintained there...
- Activation of NF-kappa B by the human T cell leukemia virus type I Tax oncoprotein is associated with ubiquitin-dependent relocalization of I kappa B kinaseNicole S Harhaj
Department of Microbiology and Immunology, Sylvester Comprehensive Cancer Center, The University of Miami, Miller School of Medicine, Miami, Florida 33136, USA
J Biol Chem 282:4185-92. 2007..Together, these results demonstrate that Tax induces relocalization of the IKK complex in a ubiquitin-dependent manner, and dynamic changes in the subcellular localization of the IKK complex may be critical for Tax function...
- The Golgi apparatus: lessons from DrosophilaVangelis Kondylis
Cell Microscopy Centre, Department of Cell Biology, UMC Utrecht, AZU H02 313, Heidelberglaan 100, Utrecht, The Netherlands
FEBS Lett 583:3827-38. 2009..issues including studies of the secretory pathway, and more specifically on the functional integrity of the Golgi apparatus. A number of advances have been made that are reviewed below...
- Alpha-synuclein blocks ER-Golgi traffic and Rab1 rescues neuron loss in Parkinson's modelsAntony A Cooper
School of Biological Sciences, University of Missouri Kansas City, Kansas City, MO 64110, USA
Science 313:324-8. 2006..Thus, synucleinopathies may result from disruptions in basic cellular functions that interface with the unique biology of particular neurons to make them especially vulnerable...
- CERT-mediated trafficking of ceramideKentaro Hanada
Department of Biochemistry, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku ku, Tokyo 162 8640, Japan
Biochim Biophys Acta 1791:684-91. 2009..catalyzing inter-membrane transfer of ceramide, ii) a pleckstrin homology domain, which serves to target the Golgi apparatus, iii) a FFAT motif which interacts with the ER-resident membrane protein VAP, and iv) a serine-repeat motif, ..
- Intracellular localization and in vivo trafficking of p24A and p23R Blum
Institute of Physiology II, Medical Biochemistry and Department of Anatomy, University of the Saarland, D 66421 Homburg Saar, Germany
J Cell Sci 112:537-48. 1999..This study therefore gives new insights into the localization and trafficking behavior of the two COPI-binding proteins p24A and p23...
- Ligand-regulated transport of the Menkes copper P-type ATPase efflux pump from the Golgi apparatus to the plasma membrane: a novel mechanism of regulated traffickingM J Petris
Department of Genetics, University of Melbourne, Parkville, Victoria, Australia
EMBO J 15:6084-95. 1996..Immunofluorescence studies demonstrated that MNK is predominantly localized to the Golgi apparatus of cells in basal medium...
- Mutant huntingtin impairs the post-Golgi trafficking of brain-derived neurotrophic factor but not its Val66Met polymorphismDaniel del Toro
Departament de Biologia Cel lular i Anatomia Patològica, Facultat de Medicina, Institut d Investigacions Biomediques August Pi i Sunyer, Universitat de Barcelona, 08036 Barcelona, Spain
J Neurosci 26:12748-57. 2006..recovery after photobleaching (FRAP) and inverse FRAP analysis showed retention of Met-BDNF in the Golgi apparatus with respect to Val-BDNF in wild-type cells...
- Flotillin-1/reggie-2 traffics to surface raft domains via a novel golgi-independent pathway. Identification of a novel membrane targeting domain and a role for palmitoylationIsabel C Morrow
Institute for Molecular Bioscience and Centre for Functional and Applied Genomics, University of Queensland, St Lucia, Australia
J Biol Chem 277:48834-41. 2002..The results identify a novel targeting mechanism for plasma membrane association of flotillin-1 involving a Golgi-independent trafficking pathway, the PHB domain, and palmitoylation...
- Identification of new Golgi complex specific proteins by direct organelle proteomic analysisMaarit S Takatalo
Department of Bio- and Environmental Sciences, Division of Biochemistry, University of Helsinki, Finland
Proteomics 6:3502-8. 2006..The Golgi localization of two of these newly identified proteins was also confirmed by indirect immunofluorescence...
- Golgi polarization in a strong electric fieldJin Pu
Biomedical Sciences, School of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, UK
J Cell Sci 118:1117-28. 2005Directional cell migration requires proper cell polarization. The redistribution of the Golgi apparatus is an important event in the polarization and migration of many types of cells, as a polarized Golgi supplies membrane components for ..
- Coronin 7, the mammalian POD-1 homologue, localizes to the Golgi apparatusVasily Rybakin
Center for Biochemistry, Institute of Biochemistry I, Medical Faculty, University of Cologne, Joseph Stelzmann Str 52, D 50931 Cologne, Germany
FEBS Lett 573:161-7. 2004..Crn7 is the first coronin protein proven to localize to the Golgi membrane. We conclude that it plays a role in the organization of intracellular membrane compartments and vesicular trafficking rather than in remodeling the cytoskeleton...
- Oncogenic targeting of an activated tyrosine kinase to the Golgi apparatus in a glioblastomaAlan Charest
Center for Cancer Research, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
Proc Natl Acad Sci U S A 100:916-21. 2003..A microdeletion on 6q21 results in the fusion of FIG, a gene coding for a Golgi apparatus-associated protein, to the kinase domain of the protooncogene c-ROS...
- Arabidopsis thaliana beta1,2-xylosyltransferase: an unusual glycosyltransferase with the potential to act at multiple stages of the plant N-glycosylation pathwayPeter Bencur
Department für Angewandte Pflanzenwissenschaften und Pflanzenbiotechnologie, Institut für Angewandte Genetik und Zellbiologie, Universitat fur Bodenkultur Wien, Muthgasse 18, A 1190 Wien, Austria
Biochem J 388:515-25. 2005..These experiments revealed that the substrate specificity of XylT permits the enzyme to act at multiple stages of the plant N-glycosylation pathway...
- A COPI coat subunit interacts directly with an early-Golgi localized Arf exchange factorYi Deng
Cell Biology and Metabolism Program, National Institute of Child Health and Human Development, NIH, Bethesda, Maryland 20892, USA
EMBO Rep 10:58-64. 2009..In this way, a specific output on Arf1 activation can be programmed before the exchange reaction by the GEF itself...
- Unconventional mechanisms of protein transport to the cell surface of eukaryotic cellsWalter Nickel
Heidelberg University Biochemistry Center BZH 69120 Heidelberg, Germany
Annu Rev Cell Dev Biol 24:287-308. 2008..membrane proteins to the cell surface require transit into and through the endoplasmic reticulum and the Golgi apparatus. Signal peptides or transmembrane domains target proteins for translocation into the lumen or insertion into ..
- Metabolism, cell surface organization, and diseaseJames W Dennis
Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario M5G 1X5, Canada
Cell 139:1229-41. 2009..Congenital disorders of glycosylation provide insight as extreme hypomorphisms, whereas milder deficiencies may encompass many common chronic conditions, including autoimmunity, metabolic syndrome, and aging...
- Yeast rab GTPase-activating protein Gyp1p localizes to the Golgi apparatus and is a negative regulator of Ypt1pL L Du
Department of Molecular Biophysics, Yale University School of Medicine, New Haven, Connecticut 06520, USA
Mol Biol Cell 12:1215-26. 2001..These results establish that Gyp1p functions on the Golgi as a negative regulator of Ypt1p...
- Glucosylceramide biosynthesis is involved in Golgi morphology and protein secretion in plant cellsSu Melser
Université V Segalen Bordeaux 2, Laboratoire de Biogenese Membranaire, CNRS UMR 5200, 146, rue Leo Saignat, 33076 Bordeaux Cedex, France
Traffic 11:479-90. 2010....
- Golgi complex reorganization during muscle differentiation: visualization in living cells and mechanismZ Lu
Laboratory of Neurobiology, National Institute of Neurological Disorders and Stroke, Bethesda, Maryland 20892-4062, USA
Mol Biol Cell 12:795-808. 2001..Thus, changes in GC organization that initially appear specific to muscle cells, in fact use pathways common to all mammalian cells...
- Golgi localisation of GMAP210 requires two distinct cis-membrane binding mechanismsJesus Cardenas
CABIMER CSIC, Seville, Spain
BMC Biol 7:56. 2009The Golgi apparatus in mammals appears as a ribbon made up of interconnected stacks of flattened cisternae that is positioned close to the centrosome in a microtubule-dependent manner...
- Expression of the novel Golgi protein GoPro49 is developmentally regulated during mesenchymal differentiationMaarit Takatalo
Department of Biological and Environmental Sciences, Division of Biochemistry, University of Helsinki, Helsinki, Finland
Dev Dyn 237:2243-55. 2008..This is the first time a Golgi membrane protein is shown to be expressed in a developmentally regulated manner during mesenchymal and cartilage development in mammals...
- Glycosphingolipid requirements for endosome-to-Golgi transport of Shiga toxinHilde Raa
Centre for Cancer Biomedicine, Faculty Division Norwegian Radium Hospital, University of Oslo, 0316 Oslo, Norway
Traffic 10:868-82. 2009..To enter the cytosol, Shiga toxin is dependent on endocytic uptake, retrograde transport to the Golgi apparatus and further to the endoplasmic reticulum before translocation of the enzymatically active moiety to the ..
- Biogenesis of Golgi stacks in imaginal discs of Drosophila melanogasterV Kondylis
The Wellcome Trust Centre for Cell Biology, Institute of Cell and Molecular Biology, University of Edinburgh, Edinburgh, EH9 3JR, Scotland, United Kingdom
Mol Biol Cell 12:2308-27. 2001..Taken together, these results suggest that we have developed an in vivo system to study the ecdysone-triggered Golgi stack biogenesis...
- Dual role of the fringe connection gene in both heparan sulphate and fringe-dependent signalling eventsE M Selva
Department of Genetics, Howard Hughes Medical Institute, Harvard Medical School, Boston, MA 02115, USA
Nat Cell Biol 3:809-15. 2001..Embryos with the frc mutation display defects in Wingless, Hedgehog and fibroblast growth factor signalling. Clonal analysis shows that fringe-dependent Notch signalling is disrupted in frc mutant tissue...
- Deletion mutants of AP-1 adaptin subunits display distinct phenotypes in fission yeastYan Ma
Division of Molecular Pharmacology and Pharmacogenomics, Department of Biochemistry and Molecular Biology, Kobe University Graduate School of Medicine, Kusunoki cho, Chuo Ku, Kobe 650 0017, Japan
Genes Cells 14:1015-28. 2009..Altogether, results suggest that the four subunits are all essential for the heterotetrameric complex formation and for the AP-1 function in exit transport from endosomes...
- Nascent lipidated apolipoprotein B is transported to the Golgi as an incompletely folded intermediate as probed by its association with network of endoplasmic reticulum molecular chaperones, GRP94, ERp72, BiP, calreticulin, and cyclophilin BJianying Zhang
Department of Physiology and Biophysics, Center for Advanced Biomedical Research, Boston University School of Medicine, Boston, Massachusetts 02118, USA
J Biol Chem 278:7459-68. 2003....
- Src kinase regulates the integrity and function of the Golgi apparatus via activation of dynamin 2Shaun G Weller
Department of Biochemistry and Molecular Biology and the Miles and Shirley Fiterman Center for Digestive Diseases, Mayo Clinic, Rochester, MN, 55905, USA
Proc Natl Acad Sci U S A 107:5863-8. 2010The size and integrity of the Golgi apparatus is maintained via a tightly controlled regulation of membrane traffic using a variety of different signaling and cytoskeletal proteins...
- The Golgi and the centrosome: building a functional partnershipChristine Sutterlin
Department of Developmental and Cell Biology, University of California, Irvine, Irvine, CA 92697, USA
J Cell Biol 188:621-8. 2010The mammalian Golgi apparatus is characterized by a ribbon-like organization adjacent to the centrosome during interphase and extensive fragmentation and dispersal away from the centrosome during mitosis...
- The SQV-1 UDP-glucuronic acid decarboxylase and the SQV-7 nucleotide-sugar transporter may act in the Golgi apparatus to affect Caenorhabditis elegans vulval morphogenesis and embryonic developmentHo Yon Hwang
Howard Hughes Medical Institute, Department of Biology, 68 425, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
Proc Natl Acad Sci U S A 99:14218-23. 2002..compartments and colocalizes with the SQV-7 nucleotide-sugar transporter, which probably acts in the Golgi apparatus. SQV-1 and SQV-7 are both expressed in the vulva and in oocytes, where they likely act in vulval ..
- Identification of different itineraries and retromer components for endosome-to-Golgi transport of TGN38 and Shiga toxinZi Zhao Lieu
The Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Melbourne, Victoria 3010, Australia
Eur J Cell Biol 89:379-93. 2010..Overall, our data have identified different itineraries for the retrograde transport of Shiga toxin and TGN38 and distinct retromer components that regulate the transport of these cargos...
- A molecular specificity code for the three mammalian KDEL receptorsIrina Raykhel
Department of Biochemistry, Biocenter Oulu, University of Oulu, 90570 Oulu, Finland
J Cell Biol 179:1193-204. 2007..This motif interacts with KDEL receptors localized in the intermediate compartment and Golgi apparatus. Such binding triggers retrieval back to the ER via a coat protein I-dependent pathway...
- Microtubule involvement in NIH 3T3 Golgi and MTOC polarity establishmentJuana Magdalena
School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK
J Cell Sci 116:743-56. 2003..In addition, this study indicates that MTOC and Golgi polarization events are separately controlled...
- Multiple sorting pathways between the late Golgi and the vacuole in yeastE Conibear
Institute of Molecular Biology, University of Oregon, Eugene, OR 97403 1229, USA
Biochim Biophys Acta 1404:211-30. 1998..Progress has also been made in further elucidating the members of the SNARE complexes that act in Golgi-to-PVC transport as well as those that mediate fusion with the vacuole...
- Insig required for sterol-mediated inhibition of Scap/SREBP binding to COPII proteins in vitroLi Ping Sun
Department of Molecular Genetics, University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 75390 9046, USA
J Biol Chem 280:26483-90. 2005..This hexapeptide acts as a sterol-regulated ER sorting signal. These studies define the biochemical parameters responsible for regulated sorting of an ER membrane protein into COPII-coated vesicles...
- Cellular internalization of cytolethal distending toxin: a new end to a known pathwayLina Guerra
Microbiology and Tumorbiology Center, Karolinska Institutet, Stockholm, Sweden
Cell Microbiol 7:921-34. 2005....
- Distance-Dependent Structure and Function of Neuronal DendritesKristen Harris; Fiscal Year: 2007..that host excitory synapses, including microtubules for transport, polyribosomes for protein synthesis, Golgi apparatus for posttranslational modifications, endosomes for membrane recycling, and smooth endoplasmic reticulum (SER) ..
- Distance-Dependent Structure and Function of Neuronal DendritesKristen M Harris; Fiscal Year: 2010..that host excitory synapses, including microtubules for transport, polyribosomes for protein synthesis, Golgi apparatus for posttranslational modifications, endosomes for membrane recycling, and smooth endoplasmic reticulum (SER) ..
- Distance-Dependent Structure and Function of Neuronal DendritesKristen Harris; Fiscal Year: 2009..that host excitory synapses, including microtubules for transport, polyribosomes for protein synthesis, Golgi apparatus for posttranslational modifications, endosomes for membrane recycling, and smooth endoplasmic reticulum (SER) ..
- Membrane Protein Trafficking in PhotoreceptorsWolfgang Baehr; Fiscal Year: 2010..Integral membrane proteins are synthesized by ER-associated ribosomes and exported to the Golgi apparatus. Prenylated or acylated peripheral membrane proteins are synthesized in the cytosol and become ER-associated...
- Membrane Protein Trafficking in PhotoreceptorsWolfgang Baehr; Fiscal Year: 2011..Integral membrane proteins are synthesized by ER-associated ribosomes and exported to the Golgi apparatus. Prenylated or acylated peripheral membrane proteins are synthesized in the cytosol and become ER-associated...
- Role and Mechanism of Bypass Pathway TraffickingAdam Linstedt; Fiscal Year: 2009..Protein and lipid cargo originating at the cell surface or Golgi apparatus gain access to the pathway by moving to early/sorting endosomes...
- Role and Mechanism of Bypass Pathway TraffickingAdam D Linstedt; Fiscal Year: 2010..Protein and lipid cargo originating at the cell surface or Golgi apparatus gain access to the pathway by moving to early/sorting endosomes...
- ON THE ROLE OF THE HPS GENE PRODUCT IN MELANOCYTESRaymond Boissy; Fiscal Year: 2002..In common, these organelles either originate and/or receive specific glycoproteins from the Golgi apparatus. Thus it is hypothesized that the HPS gene product regulates selective trafficking from the trans Golgi ..
- MOLECULAR STUDIES OF SELECTIVE PROTEIN TRANSPORTGregory Payne; Fiscal Year: 1993..proteins, receptor-mediated endocytosis of the mating peptide alpha-factor, and sorting of proteins from the Golgi apparatus to the lysosome-like vacuole...
- MOLECULAR STUDIES OF SELECTIVE PROTEIN TRANSPORTGregory Payne; Fiscal Year: 2000..Together, these approaches will identify previously unrecognized components of the selective protein transport machinery and address the specific roles played by each identified participant. ..
- MICROTUBULE-BASED TRANSPORT IN LACRIMAL GLAND FUNCTIONSarah F Hamm Alvarez; Fiscal Year: 2010....
- Amyloid Beta-Protein: ApoE and Cholesterol HomeostasisWELLINGTON WOOD; Fiscal Year: 2007..Determine if the neuronal apoE4 and apoE-null phenotype can be "rescued" by incubation with astrocytes of apoE2, apoE3, or wildtype mice. In some experiments astrocytes will be treated with ABeta1-42. ..
- Amyloid Beta-Protein: ApoE and Cholesterol HomeostasisWELLINGTON WOOD; Fiscal Year: 2009..Determine if the neuronal apoE4 and apoE-null phenotype can be "rescued" by incubation with astrocytes of apoE2, apoE3, or wildtype mice. In some experiments astrocytes will be treated with ABeta1-42. ..
- The Role of Arf GTPases in Endocytosis and Postendocytic TransportJames Casanova; Fiscal Year: 2009..It is well established that Arfs promote the assembly of clathrin-coated vesicles in the Golgi apparatus and on endosomes, but their role in clathrin- mediated endocytosis at the plasma membrane is poorly ..
- The Role of Arf GTPases in Endocytosis and Postendocytic TransportJames E Casanova; Fiscal Year: 2010..It is well established that Arfs promote the assembly of clathrin-coated vesicles in the Golgi apparatus and on endosomes, but their role in clathrin- mediated endocytosis at the plasma membrane is poorly ..
- Intraflagellar Transport Proteins in MiceGregory J Pazour; Fiscal Year: 2011..We have shown that IFT20 is required for ciliary assembly and that it cycles between the Golgi apparatus and the cilium. At the Golgi membrane, IFT20 is complexed with the golgin protein GMAP210...
- GLYCOSYLTRANSFERASE FUNCTION DURING FERTILIZATIONBARRY SHUR; Fiscal Year: 2006..Finally, in the third Aim, we will continue our analysis of GalT-mediated signal transduction leading to the acrosome reaction. ..
- SORTING AND TRANSPORT OF YEAST MEMBRANE PROTEINSTom Stevens; Fiscal Year: 2009..Understanding V-ATPase function will provide important insights into diseases of the kidney (renal tubular acidosis), bone diseases (osteopetrosis), and in tumor metastasis, and new drug targets should arise from our studies. ..
- SORTING AND TRANSPORT OF YEAST MEMBRANE PROTEINSTOM HALL STEVENS; Fiscal Year: 2010..Understanding V-ATPase function will provide important insights into diseases of the kidney (renal tubular acidosis), bone diseases (osteopetrosis), and in tumor metastasis, and new drug targets should arise from our studies. ..
- BIOSYNTHESIS AND PROCESSING OF PEPTIDE HORMONEDennis Shields; Fiscal Year: 2007..Aim I1: The Regulation of Golgi PI(4,5)P2 synthesis: Surprisingly, the Golgi apparatus appears to possess little PI(4,5)P2 and its level maybe tightly controlled, in part, by a potent Pl(4,5)P2-5-..
- Mannosidase inhibitors as therapeutics for glycoprotein misfolding diseasesKelley Moremen; Fiscal Year: 2007....
- HUMAN ALKALINE PHYTOCERAMIDASE CONTROL OF ANGIOGENESISCungui Mao; Fiscal Year: 2002..studies, we demonstrate that haPHC 1) is highly expressed in placenta and heart and localized to the Golgi apparatus and endoplasmic reticulum; 2) hydrolyzes NBD-Clz-phytoceramide preferentially in vitro, and 3) is activated ..
- Drs2p Function in Clathrin-coated Vesicle BuddingTODD GRAHAM; Fiscal Year: 2004..Loss of this asymmetric distribution of PS in blood cells stimulates clot formation and in apoptotic cells allows for recognition and phagocytosis by macrophages. ..
- ALPHA-MANNOSIDASES IN MAMMALIAN GLYCOPROTEIN PROCESSINGKelley Moremen; Fiscal Year: 1993..Finally, a detailed examination of the expression of the alpha- mannosidases in the context of the glycosylation pathway as a whole should yield a cohesive picture of the coordinated regulation of the pathway in animal systems...
- Cyclic AMP Mediation of Epithelial Cell FunctionJAMES RICHARD GOLDENRING; Fiscal Year: 2010..AKAP350 is localized to both centrosomes and the Golgi apparatus, and we have demonstrated that depletion of AKAP350A with siRNA leads to disruption of the Golgi structure as ..