vacuoles

Summary

Summary: Any spaces or cavities within a cell. They may function in digestion, storage, secretion, or excretion.

Top Publications

  1. ncbi Bafilomycin A1 prevents maturation of autophagic vacuoles by inhibiting fusion between autophagosomes and lysosomes in rat hepatoma cell line, H-4-II-E cells
    A Yamamoto
    Department of Physiology and Liver Research Center Cell Biology, Kansai Medical University, Osaka, Japan
    Cell Struct Funct 23:33-42. 1998
  2. ncbi Role for Rab7 in maturation of late autophagic vacuoles
    Stefanie Jäger
    Institute of Biochemistry, University of Kiel, Olshausenstr 40, 24098 Kiel, Germany
    J Cell Sci 117:4837-48. 2004
  3. ncbi Proteomic analysis of rhoptry organelles reveals many novel constituents for host-parasite interactions in Toxoplasma gondii
    Peter J Bradley
    Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California 94305, USA
    J Biol Chem 280:34245-58. 2005
  4. pmc The reversible modification regulates the membrane-binding state of Apg8/Aut7 essential for autophagy and the cytoplasm to vacuole targeting pathway
    T Kirisako
    Department of Cell Biology, National Institute for Basic Biology, Okazaki 444 8585, Japan
    J Cell Biol 151:263-76. 2000
  5. ncbi Extensive involvement of autophagy in Alzheimer disease: an immuno-electron microscopy study
    Ralph A Nixon
    Center for Dementia Research, Nathan Kline Institute for Psychiatric Research, New York University School of Medicine, Orangeburg, New York 10962, USA
    J Neuropathol Exp Neurol 64:113-22. 2005
  6. pmc Determinants of GBP recruitment to Toxoplasma gondii vacuoles and the parasitic factors that control it
    Sebastian Virreira Winter
    Whitehead Institute for Biomedical Research, Cambridge, Massachusetts, United States of America
    PLoS ONE 6:e24434. 2011
  7. pmc Piecemeal microautophagy of nucleus in Saccharomyces cerevisiae
    Paul Roberts
    Department of Biology, University of Rochester, Rochester, New York 14627, USA
    Mol Biol Cell 14:129-41. 2003
  8. pmc Ankyrin repeat proteins comprise a diverse family of bacterial type IV effectors
    Xiaoxiao Pan
    Section of Microbial Pathogenesis, Yale University School of Medicine, 295 Congress Avenue, New Haven, CT 06536, USA
    Science 320:1651-4. 2008
  9. ncbi Isolation and characterization of autophagy-defective mutants of Saccharomyces cerevisiae
    M Tsukada
    Department of Biology, College of Arts and Sciences, University of Tokyo, Japan
    FEBS Lett 333:169-74. 1993
  10. ncbi A novel assay to study autophagy: regulation of autophagosome vacuole size by amino acid deprivation
    D B Munafó
    Laboratorio de Biología Celular y Molecular Instituto de Histología y Embriología, Facultad de Ciencias Medicas, Universidad Nacional de Cuyo CONICET, Mendoza, 5500, Argentina
    J Cell Sci 114:3619-29. 2001

Detail Information

Publications285 found, 100 shown here

  1. ncbi Bafilomycin A1 prevents maturation of autophagic vacuoles by inhibiting fusion between autophagosomes and lysosomes in rat hepatoma cell line, H-4-II-E cells
    A Yamamoto
    Department of Physiology and Liver Research Center Cell Biology, Kansai Medical University, Osaka, Japan
    Cell Struct Funct 23:33-42. 1998
    ..Bafilomycin A1 also prevented the appearance of endocytosed HRP in autophagic vacuoles. These results suggested that acidification of the lumenal space of autophagosomes or lysosomes by V-ATPase is ..
  2. ncbi Role for Rab7 in maturation of late autophagic vacuoles
    Stefanie Jäger
    Institute of Biochemistry, University of Kiel, Olshausenstr 40, 24098 Kiel, Germany
    J Cell Sci 117:4837-48. 2004
    ..By electron microscopy we showed that these large aggregates corresponded to autophagic vacuoles surrounding late endosomal or lysosomal vesicles...
  3. ncbi Proteomic analysis of rhoptry organelles reveals many novel constituents for host-parasite interactions in Toxoplasma gondii
    Peter J Bradley
    Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California 94305, USA
    J Biol Chem 280:34245-58. 2005
    ....
  4. pmc The reversible modification regulates the membrane-binding state of Apg8/Aut7 essential for autophagy and the cytoplasm to vacuole targeting pathway
    T Kirisako
    Department of Cell Biology, National Institute for Basic Biology, Okazaki 444 8585, Japan
    J Cell Biol 151:263-76. 2000
    ..A reaction similar to ubiquitination is probably involved in the second modification. The reversible modification of Apg8 appears to be coupled to the membrane dynamics of autophagy and the Cvt pathway...
  5. ncbi Extensive involvement of autophagy in Alzheimer disease: an immuno-electron microscopy study
    Ralph A Nixon
    Center for Dementia Research, Nathan Kline Institute for Psychiatric Research, New York University School of Medicine, Orangeburg, New York 10962, USA
    J Neuropathol Exp Neurol 64:113-22. 2005
    ..neocortical biopsies from AD brain, we unequivocally identified autophagosomes and other prelysosomal autophagic vacuoles (AVs), which were morphologically and biochemically similar to AVs highly purified from mouse liver...
  6. pmc Determinants of GBP recruitment to Toxoplasma gondii vacuoles and the parasitic factors that control it
    Sebastian Virreira Winter
    Whitehead Institute for Biomedical Research, Cambridge, Massachusetts, United States of America
    PLoS ONE 6:e24434. 2011
    ..gondii in animals may rely in part on recognition by GBPs. However, phagosomes or vacuoles containing Trypanosoma cruzi did not recruit mGBP1...
  7. pmc Piecemeal microautophagy of nucleus in Saccharomyces cerevisiae
    Paul Roberts
    Department of Biology, University of Rochester, Rochester, New York 14627, USA
    Mol Biol Cell 14:129-41. 2003
    ..These results introduce a novel mode of selective microautophagy that targets nonessential components of the yeast nucleus for degradation and recycling in the vacuole...
  8. pmc Ankyrin repeat proteins comprise a diverse family of bacterial type IV effectors
    Xiaoxiao Pan
    Section of Microbial Pathogenesis, Yale University School of Medicine, 295 Congress Avenue, New Haven, CT 06536, USA
    Science 320:1651-4. 2008
    ..pneumophila-containing vacuole with late endosomes after infection of macrophages, which demonstrates that Ank proteins have effector functions important for bacterial infection of eukaryotic host cells...
  9. ncbi Isolation and characterization of autophagy-defective mutants of Saccharomyces cerevisiae
    M Tsukada
    Department of Biology, College of Arts and Sciences, University of Tokyo, Japan
    FEBS Lett 333:169-74. 1993
    ..A mutant designated as apg1 (autophagy) defective in accumulation of autophagic bodies in the vacuoles was isolated by selection using a light microscope from a mutagenized proteinase-deficient strain...
  10. ncbi A novel assay to study autophagy: regulation of autophagosome vacuole size by amino acid deprivation
    D B Munafó
    Laboratorio de Biología Celular y Molecular Instituto de Histología y Embriología, Facultad de Ciencias Medicas, Universidad Nacional de Cuyo CONICET, Mendoza, 5500, Argentina
    J Cell Sci 114:3619-29. 2001
    ..inhibitor of PI3-kinases that blocks autophagy at an early stage, inhibited the accumulation of MDC in autophagic vacuoles. We also found that treatment of the cells with N-ethylmaleimide (NEM), an agent known to inhibit several ..
  11. pmc Protein sorting in Saccharomyces cerevisiae: isolation of mutants defective in the delivery and processing of multiple vacuolar hydrolases
    J S Robinson
    Division of Biology, California Institute of Technology, Pasadena 91125
    Mol Cell Biol 8:4936-48. 1988
    ..Together, these data indicate that more than 50 gene products are involved, directly or indirectly, in the process of vacuolar protein sorting...
  12. ncbi Maturation of autophagic vacuoles in Mammalian cells
    Eeva Liisa Eskelinen
    Institute of Biochemistry, University of Kiel, Germany
    Autophagy 1:1-10. 2005
    ..After their formation as double-membraned vacuoles containing cytoplasmic material, autophagic vacuoles or autophagosomes undergo a stepwise maturation including ..
  13. pmc Vacuolar and plasma membrane proton pumps collaborate to achieve cytosolic pH homeostasis in yeast
    Gloria A Martínez-Muñoz
    Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, Syracuse, NY 13210, USA
    J Biol Chem 283:20309-19. 2008
    ....
  14. ncbi Tsg101 and the vacuolar protein sorting pathway are essential for HIV-1 budding
    J E Garrus
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, UT 84132, USA
    Cell 107:55-65. 2001
    ..These observations suggest that retroviruses bud by appropriating cellular machinery normally used in the Vps pathway to form multivesicular bodies...
  15. pmc Molecular architecture of the multisubunit homotypic fusion and vacuole protein sorting (HOPS) tethering complex
    Cornelia Bröcker
    Biochemistry Section, Department of Biology, University of Osnabruck, 49076 Osnabruck, Germany
    Proc Natl Acad Sci U S A 109:1991-6. 2012
    ..Vps11 and Vps18 connect head and tail. Our data suggest that HOPS bridges Ypt7-positive membranes and chaperones SNAREs at fusion sites...
  16. ncbi Autophagy controls Salmonella infection in response to damage to the Salmonella-containing vacuole
    Cheryl L Birmingham
    Infection, Immunity, Injury and Repair Program, Hospital for Sick Children, Toronto, Ontario M5G 1X8, Canada
    J Biol Chem 281:11374-83. 2006
    ..This serves to retain intracellular S. Typhimurium within vacuoles early after infection to protect the cytosol from bacterial colonization...
  17. pmc Cargo proteins facilitate the formation of transport vesicles in the cytoplasm to vacuole targeting pathway
    Takahiro Shintani
    Life Sciences Institute and Departments of Molecular, Cellular, and Developmental Biology and Biological Chemistry, University of Michigan, Ann Arbor, Michigan 48109, USA
    J Biol Chem 279:29889-94. 2004
    ....
  18. pmc Processing of ATG8s, ubiquitin-like proteins, and their deconjugation by ATG4s are essential for plant autophagy
    Kohki Yoshimoto
    Department of Cell Biology, National Institute for Basic Biology, Myodaiji cho, Okazaki 444 8585, Japan
    Plant Cell 16:2967-83. 2004
    ..In addition, we detected autophagic bodies in the vacuoles of wild-type roots but not in those of atg4a4b-1 in the presence of concanamycin A, a V-ATPase inhibitor...
  19. pmc Ion exchangers NHX1 and NHX2 mediate active potassium uptake into vacuoles to regulate cell turgor and stomatal function in Arabidopsis
    Verónica Barragán
    Instituto de Recursos Naturales y Agrobiologia, Consejo Superior de Investigaciones Cientificas, Sevilla 41012, Spain
    Plant Cell 24:1127-42. 2012
    ..Together, these results show that tonoplast-localized NHX proteins are essential for active K(+) uptake at the tonoplast, for turgor regulation, and for stomatal function...
  20. ncbi Induction of lysosomal dilatation, arrested autophagy, and cell death by chloroquine in cultured ARPE-19 cells
    Young Hee Yoon
    Department of Ophthalmology, University of Ulsan, College of Medicine, Seoul, Korea
    Invest Ophthalmol Vis Sci 51:6030-7. 2010
    ..To characterize and investigate the mechanism of chloroquine (CQ) retinotoxicity in human retinal pigment epithelium-derived ARPE-19 cells...
  21. pmc Ginsenoside Rh2 induces apoptosis and paraptosis-like cell death in colorectal cancer cells through activation of p53
    Binghui Li
    Ben May Department for Cancer Research, The University of Chicago, IL 60637, United States
    Cancer Lett 301:185-92. 2011
    ..the caspase-independent paraptosis, a type of cell death that is characterized by the accumulation of cytoplasmic vacuoles. Treatment of cells with Rh2 activated the p53 pathway and significantly increased the levels of the pro-..
  22. ncbi Migration of Plasmodium sporozoites through cells before infection
    M M Mota
    Department of Pathology, New York University School of Medicine, 341 East 25 Street, New York, NY 10010, USA
    Science 291:141-4. 2001
    ..Sporozoite migration through several cells in the mammalian host appears to be essential for the completion of the life cycle...
  23. ncbi The CORVET tethering complex interacts with the yeast Rab5 homolog Vps21 and is involved in endo-lysosomal biogenesis
    Karolina Peplowska
    University of Osnabruck, Department of Biology, Biochemistry Section, Barbarastrasse 13, 49076 Osnabruck, Germany
    Dev Cell 12:739-50. 2007
    ..Our data suggest that modular assembled tethering complexes define organelle biogenesis in the endocytic pathway...
  24. ncbi Subcellular discharge of a serine protease mediates release of invasive malaria parasites from host erythrocytes
    Sharon Yeoh
    Division of Parasitology, National Institute for Medical Research, Mill Hill, London NW7 1AA, UK
    Cell 131:1072-83. 2007
    ..Our findings reveal the presence in the malarial parasitophorous vacuole of a regulated, PfSUB1-mediated proteolytic processing event required for release of viable parasites from the host erythrocyte...
  25. pmc Disruption of Toxoplasma gondii parasitophorous vacuoles by the mouse p47-resistance GTPases
    Sascha Martens
    Institute for Genetics, University of Cologne, Cologne, Germany
    PLoS Pathog 1:e24. 2005
    ..We show that the p47 GTPases, including IIGP1, accumulate at vacuoles containing T. gondii. The accumulation is GTP-dependent and requires live parasites...
  26. ncbi Membrane fusion: five lipids, four SNAREs, three chaperones, two nucleotides, and a Rab, all dancing in a ring on yeast vacuoles
    William Wickner
    Department of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire 03755 3844, USA
    Annu Rev Cell Dev Biol 26:115-36. 2010
    ....
  27. ncbi Accumulation of autophagic vacuoles and cardiomyopathy in LAMP-2-deficient mice
    Y Tanaka
    Zentrum Biochemie und Molekulare Zellbiologie, Abt Biochemie II, Universitat Gottingen, Germany
    Nature 406:902-6. 2000
    ..Ultrastructurally, there is extensive accumulation of autophagic vacuoles in many tissues including liver, pancreas, spleen, kidney and skeletal and heart muscle...
  28. pmc Autophagy machinery mediates macroendocytic processing and entotic cell death by targeting single membranes
    Oliver Florey
    Cell Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10065, USA
    Nat Cell Biol 13:1335-43. 2011
    ..Here we report the modification of single-membrane vacuoles in cells by autophagy proteins...
  29. pmc Dot/Icm type IVB secretion system requirements for Coxiella burnetii growth in human macrophages
    Paul A Beare
    Coxiella Pathogenesis Section, Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA
    MBio 2:e00175-11. 2011
    ....
  30. pmc The molecular machinery of autophagy: unanswered questions
    Daniel J Klionsky
    Life Sciences Institute, Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA
    J Cell Sci 118:7-18. 2005
    ....
  31. ncbi The TOR and EGO protein complexes orchestrate microautophagy in yeast
    Frédérique Dubouloz
    Department of Microbiology and Molecular Medicine, University of Geneva Medical School, 1211 Geneva 4, Switzerland
    Mol Cell 19:15-26. 2005
    ..Moreover, large-scale genetic analyses of the EGO complex confirm the existence of a growth control mechanism originating at the vacuolar membrane and pinpoint the amino acid glutamine as a key metabolite in TOR signaling...
  32. ncbi Food vacuole-associated lipid bodies and heterogeneous lipid environments in the malaria parasite, Plasmodium falciparum
    Katherine E Jackson
    Department of Biochemistry, La Trobe University, Melbourne 3086, Victoria, Australia
    Mol Microbiol 54:109-22. 2004
    ..Electron microscopy and isolation of food vacuoles confirmed the size of these particles and their intimate association respectively...
  33. ncbi Cellular mechanisms for heavy metal detoxification and tolerance
    J L Hall
    School of Biological Sciences, University of Southampton, Biomedical Sciences Building, Southampton SO16 7PX, UK
    J Exp Bot 53:1-11. 2002
    ..This review provides a broad overview of the evidence for an involvement of each mechanism in heavy metal detoxification and tolerance...
  34. pmc Piecemeal microautophagy of the nucleus requires the core macroautophagy genes
    R Krick
    Center of Biochemistry and Molecular Cell Biology, Georg August University, D 37073 Gottingen, Germany
    Mol Biol Cell 19:4492-505. 2008
    ..PMN does not require the complete vacuole homotypic fusion genes. We conclude that a spectrum of ATG genes is required for the terminal vacuole enclosure and fusion stages of PMN...
  35. ncbi ATG genes involved in non-selective autophagy are conserved from yeast to man, but the selective Cvt and pexophagy pathways also require organism-specific genes
    Wiebe H Meijer
    Eukaryotic Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Haren, The Netherlands
    Autophagy 3:106-16. 2007
    ..This suggests that in fungi an organism-specific form of selective autophagy may occur, for which specialized Atg proteins have evolved...
  36. ncbi Membrane dynamics and fusion at late endosomes and vacuoles--Rab regulation, multisubunit tethering complexes and SNAREs
    Nadine Epp
    University of Osnabruck, Faculty of Biology and Chemistry, Department of Biochemistry, Barbarastrasse 13, 49076 Osnabruck, Germany
    Eur J Cell Biol 90:779-85. 2011
    Membrane fusion at late endosomes and vacuoles depends on a conserved machinery, which includes Rab GTPases, their binding to tethering complexes and SNAREs. Fusion is initiated by the interaction of Rabs with tethering complexes...
  37. pmc Genomic screen for vacuolar protein sorting genes in Saccharomyces cerevisiae
    Cecilia J Bonangelino
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Mol Biol Cell 13:2486-501. 2002
    ..The identification and phenotypic characterization of these novel mutants provide new insights into the mechanisms of vacuolar protein sorting, most notably the probable involvement of the actin cytoskeleton in this process...
  38. pmc Endosome to Golgi retrieval of the vacuolar protein sorting receptor, Vps10p, requires the function of the VPS29, VPS30, and VPS35 gene products
    M N Seaman
    Division of Cellular and Molecular Medicine, University of California, San Diego, School of Medicine, La Jolla 92093 0668, USA
    J Cell Biol 137:79-92. 1997
    ....
  39. pmc Purification of active HOPS complex reveals its affinities for phosphoinositides and the SNARE Vam7p
    Christopher Stroupe
    Department of Biochemistry, Dartmouth Medical School, Hanover, NH 03755 3844, USA
    EMBO J 25:1579-89. 2006
    ..support HOPS complex association with the vacuole and explain its enrichment at the same microdomains on docked vacuoles as phosphoinositides, Ypt7p, Vam7p, and the other SNARE proteins...
  40. pmc Multivesicular bodies mature from the trans-Golgi network/early endosome in Arabidopsis
    David Scheuring
    Plant Cell Biology, Centre for Organismal Studies, University of Heidelberg, 69120 Heidelberg, Germany
    Plant Cell 23:3463-81. 2011
    ..Here, we present evidence that post-TGN transport toward lytic vacuoles occurs independently of clathrin and that MVBs/LEs are derived from the TGN/EE through maturation...
  41. pmc Toxoplasma evacuoles: a two-step process of secretion and fusion forms the parasitophorous vacuole
    S Hakansson
    Department of Molecular Microbiology, Washington University School of Medicine, Box 8230, 660 S Euclid Avenue, St Louis, MO 63110, USA
    EMBO J 20:3132-44. 2001
    ..In contrast, evacuoles were recruited to pre-existing, parasite-containing vacuoles and were capable of fusing and delivering their contents to these compartments...
  42. pmc Electrospray ionization tandem mass spectrometry (ESI-MS/MS) analysis of the lipid molecular species composition of yeast subcellular membranes reveals acyl chain-based sorting/remodeling of distinct molecular species en route to the plasma membrane
    R Schneiter
    Spezialforschungsbereich Biomembrane Research Center, Institut fur Biochemie und Lebensmittelchemie, Technische Universitat Graz, A 8010 Graz, Austria
    J Cell Biol 146:741-54. 1999
    ..This observation suggests that acyl chain-based sorting and/or remodeling mechanisms are operating to maintain the specific lipid molecular species composition of the yeast plasma membrane...
  43. pmc Coordinated loading of IRG resistance GTPases on to the Toxoplasma gondii parasitophorous vacuole
    Aliaksandr Khaminets
    Institute for Genetics, University of Cologne, Zulpicher Strasse, Cologne 50674, Germany
    Cell Microbiol 12:939-61. 2010
    ..Loading of IRG proteins onto the vacuoles of virulent Toxoplasma strains is attenuated and the two pioneer IRGs are the most affected...
  44. pmc The Salmonella enterica serovar typhimurium translocated effectors SseJ and SifB are targeted to the Salmonella-containing vacuole
    Jeremy A Freeman
    Department of Microbiology, University of Washington, Seattle, Washington 98195, USA
    Infect Immun 71:418-27. 2003
    ..The contribution of SseJ to in vivo replication is identical in wild-type and sifA deletion backgrounds, suggesting that SseJ trafficking away from the SCV along Sifs is unnecessary for its virulence function...
  45. ncbi Autophagic vacuoles are enriched in amyloid precursor protein-secretase activities: implications for beta-amyloid peptide over-production and localization in Alzheimer's disease
    W H Yu
    Center for Dementia Research, Nathan S Kline Institute, 140 Old Orangeburg Road, Orangeburg, NY 10962, USA
    Int J Biochem Cell Biol 36:2531-40. 2004
    ..and organelle turnover, include marked accumulations of autophagy-related vesicular compartments (autophagic vacuoles or AVs) in affected neurons...
  46. pmc Apg7p/Cvt2p is required for the cytoplasm-to-vacuole targeting, macroautophagy, and peroxisome degradation pathways
    J Kim
    Section of Microbiology, University of California, Davis, California 95616, USA
    Mol Biol Cell 10:1337-51. 1999
    ....
  47. pmc Cytoplasmic lipid droplets are translocated into the lumen of the Chlamydia trachomatis parasitophorous vacuole
    Jordan L Cocchiaro
    Center for Microbial Pathogenesis, Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC 27710, USA
    Proc Natl Acad Sci U S A 105:9379-84. 2008
    ..The wholesale transport of LDs into the lumen of a parasitophorous vacuole represents a unique mechanism of organelle sequestration and subversion by a bacterial pathogen...
  48. pmc Brucella coopts the small GTPase Sar1 for intracellular replication
    Jean Celli
    Centre d immunologie de Marseille Luminy, Institut National de la Santé et de la Recherche Médicale Centre National de la Recherche Scientifique Université delaMéditerranée, 13288 Marseille Cedex 09, France
    Proc Natl Acad Sci U S A 102:1673-8. 2005
    ..investigated a putative role of the early secretory pathway in ER membrane accretion by the Brucella-containing vacuoles (BCVs)...
  49. pmc Legionella pneumophila exploits PI(4)P to anchor secreted effector proteins to the replicative vacuole
    Stefan S Weber
    Institute of Microbiology, ETH Zurich, Zurich, Switzerland
    PLoS Pathog 2:e46. 2006
    ..Legionella-containing vacuoles (LCVs) do not fuse with endosomes but recruit early secretory vesicles...
  50. pmc Apg9p/Cvt7p is an integral membrane protein required for transport vesicle formation in the Cvt and autophagy pathways
    T Noda
    Department of Cell Biology, National Institute for Basic Biology, Okazaki 444 8585, Japan
    J Cell Biol 148:465-80. 2000
    ..From these results, we propose that Apg9p/Cvt7p may serve as a marker for a specialized compartment essential for these vesicle-mediated alternative targeting pathways...
  51. ncbi Vacuolar transporters and their essential role in plant metabolism
    Enrico Martinoia
    Institute of Plant Biology, University of Zurich, Zollikerstr 107, Ch 8008 Zurich, Switzerland
    J Exp Bot 58:83-102. 2007
    Following the unequivocal demonstration that plants contain at least two types of vacuoles, scientists studying this organelle have realized that the plant 'vacuome' is far more complex than they expected...
  52. ncbi A bifunctional bacterial protein links GDI displacement to Rab1 activation
    Matthias P Machner
    Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, MA 02111, USA
    Science 318:974-7. 2007
    ..During host cell infection, recruitment of Rab1 to Legionella-containing vacuoles depended on the GDF activity of SidM...
  53. ncbi Legionella pneumophila proteins that regulate Rab1 membrane cycling
    Alyssa Ingmundson
    Section of Microbial Pathogenesis, Yale University School of Medicine, Boyer Center for Molecular Medicine, 295 Congress Avenue, New Haven, Connecticut 06536, USA
    Nature 450:365-9. 2007
    ..Thus, L. pneumophila encodes proteins that regulate three distinct biochemical reactions critical for Rab GTPase membrane cycling to redirect Rab1 to the pathogen-occupied vacuole and to control Rab1 function...
  54. ncbi Expression and secretion of Salmonella pathogenicity island-2 virulence genes in response to acidification exhibit differential requirements of a functional type III secretion apparatus and SsaL
    Brian K Coombes
    Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada
    J Biol Chem 279:49804-15. 2004
    ..These data also suggest the existence of a previously unrecognized regulatory element within SPI-2 for the "effector operon" region downstream of sseD that might demarcate the expression of translocators and effectors...
  55. ncbi Human IRGM induces autophagy to eliminate intracellular mycobacteria
    Sudha B Singh
    Department of Molecular Genetics and Microbiology, University of New Mexico School of Medicine, Albuquerque, NM 87131, USA
    Science 313:1438-41. 2006
    ..We also identified a function for a human IRG protein in the control of intracellular pathogens and report that the human Irgm1 ortholog, IRGM, plays a role in autophagy and in the reduction of intracellular bacillary load...
  56. pmc De-AMPylation of the small GTPase Rab1 by the pathogen Legionella pneumophila
    M Ramona Neunuebel
    Cell Biology and Metabolism Program, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Science 333:453-6. 2011
    ..L. pneumophila mutants lacking SidD were defective for Rab1 removal from LCVs, identifying SidD as the missing link connecting the processes of early Rab1 accumulation and subsequent Rab1 removal during infection...
  57. pmc Vacuolar and plasma membrane stripping and autophagic elimination of Toxoplasma gondii in primed effector macrophages
    Yun M Ling
    Department of Molecular Microbiology and Immunology, Division of Biology and Medicine, Brown University, and Core Research Laboratories, Rhode Island Hospital, Providence, RI 02912, USA
    J Exp Med 203:2063-71. 2006
    ..Denuded parasites were enveloped in autophagosome-like vacuoles, which ultimately fused with lysosomes...
  58. pmc HOPS initiates vacuole docking by tethering membranes before trans-SNARE complex assembly
    Christopher M Hickey
    Department of Biochemistry, Dartmouth Medical School, Hanover, NH 03755, USA
    Mol Biol Cell 21:2297-305. 2010
    ..SNAREs further stabilize the associations of HOPS-tethered membranes. HOPS then protects newly formed trans-SNARE complexes from disassembly by Sec17p/Sec18p...
  59. pmc The destination for single-pass membrane proteins is influenced markedly by the length of the hydrophobic domain
    Federica Brandizzi
    Research School of Biological and Molecular Sciences, Oxford Brookes University, Gipsy Lane Campus, OX3 0BP Headington Oxford, United Kingdom
    Plant Cell 14:1077-92. 2002
    ..Moreover, the target membrane where the chimera concentrates is not unique and depends at least in part on the length of the membrane-spanning domain...
  60. pmc A SNARE complex unique to seed plants is required for protein storage vacuole biogenesis and seed development of Arabidopsis thaliana
    Kazuo Ebine
    Department of Biological Sciences, Graduate School of Science, University of Tokyo, Bunkyo ku, Tokyo 113 0033, Japan
    Plant Cell 20:3006-21. 2008
    ..We suggest that the VAMP727 complex mediates the membrane fusion between the prevacuolar compartment and the vacuole and that this process has evolved as an essential step for seed development...
  61. pmc New insights into globoids of protein storage vacuoles in wheat aleurone using synchrotron soft X-ray microscopy
    Marjana Regvar
    University of Ljubljana, Jamnikarjeva 101, Ljubljana, Slovenia
    J Exp Bot 62:3929-39. 2011
    ..Zn, Fe, Na, Mg, Al, and P were linked to globoid structures within protein storage vacuoles with variable levels of co-localization...
  62. pmc The V-ATPase proteolipid cylinder promotes the lipid-mixing stage of SNARE-dependent fusion of yeast vacuoles
    Bernd Strasser
    Departement de Biochimie, Universite de Lausanne, Epalinges, Switzerland
    EMBO J 30:4126-41. 2011
    ..We propose that a SNARE-dependent conformational change in V(0) proteolipids might stimulate fusion by creating a hydrophobic crevice that promotes lipid reorientation and formation of a lipidic fusion pore...
  63. pmc Subunit organization and Rab interactions of Vps-C protein complexes that control endolysosomal membrane traffic
    Rachael L Plemel
    Department of Biochemistry, University of Washington, Seattle, WA 98195 3750, USA
    Mol Biol Cell 22:1353-63. 2011
    ..Biochemical and genetic experiments demonstrate the importance of these regions, revealing the Vps11 CTD as a key integrator of Vps-C complex assembly, Rab signaling, and endosomal and lysosomal traffic...
  64. ncbi Evidence for sequential action of Rab5 and Rab7 GTPases in prevacuolar organelle partitioning
    Francesca Bottanelli
    Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, Leeds, LS2 9JT, UK
    Traffic 13:338-54. 2012
    ..A model describing the sequential action of Rab5 and Rab7 GTPases is presented in the light of the current observations...
  65. ncbi Identification of autophagic vacuoles and regulators of autophagy in villous trophoblast from normal term pregnancies and in fetal growth restriction
    Sophie Curtis
    Maternal and Fetal Health Research Centre, Institute of Human Development, University of Manchester, Manchester Academic Health Science Centre, Manchester, UK
    J Matern Fetal Neonatal Med 26:339-46. 2013
    ..We conclude that autophagy in human term placenta may be involved in the placental dysfunction present in FGR...
  66. ncbi Autophagy recognizes intracellular Salmonella enterica serovar Typhimurium in damaged vacuoles
    Cheryl L Birmingham
    Infection, Immunity, Injury and Repair Program, The Hospital for Sick Children, Toronto, Ontario, Canada
    Autophagy 2:156-8. 2006
    ..Results of our in vitro infection studies are consistent with autophagy playing a role in cellular defense against S. Typhimurium that become exposed to the cytosol...
  67. pmc Cellular vacuoles induced by Mycoplasma pneumoniae CARDS toxin originate from Rab9-associated compartments
    Coreen Johnson
    Department of Microbiology and Immunology, The University of Texas Health Science Center at San Antonio, San Antonio, Texas, United States of America
    PLoS ONE 6:e22877. 2011
    ..In this study we show that vacuoles induced by recombinant CARDS (rCARDS) toxin are acidic and derive from the endocytic pathway as determined by the ..
  68. doi TDP-43 accumulation is common in myopathies with rimmed vacuoles
    Benno Kusters
    Department of Pathology, Radboud University Nijmegen Medical Center, PO 9101, 6500 HB, Nijmegen, The Netherlands
    Acta Neuropathol 117:209-11. 2009
  69. ncbi Plant vacuoles: where did they come from and where are they heading?
    Jan Zouhar
    Departamento de Genética Molecular de Plantas, Centro Nacional de Biotecnologia, Consejo Superior de Investigaciones Cientificas, E 28049 Madrid, Spain
    Curr Opin Plant Biol 12:677-84. 2009
    ..We have now tools to start answering some fundamental questions such as: How are vacuoles formed? Are all vacuoles formed similarly? Do different types of vacuoles coexist in a cell? How are proteins ..
  70. ncbi Proteome analysis of Legionella vacuoles purified by magnetic immunoseparation reveals secretory and endosomal GTPases
    Simon Urwyler
    Institute of Microbiology, Department of Biology, ETH Zurich, Zurich, Switzerland
    Traffic 10:76-87. 2009
    ..the causative agent of Legionnaires' disease, replicates in macrophages and amoebae within 'Legionella-containing vacuoles' (LCVs), which communicate with the early secretory pathway and the endoplasmic reticulum...
  71. ncbi Vacuolar transporters in their physiological context
    Enrico Martinoia
    Institute of Plant Biology, University of Zurich, Zurich, Switzerland
    Annu Rev Plant Biol 63:183-213. 2012
    b>Vacuoles in vegetative tissues allow the plant surface to expand by accumulating energetically cheap inorganic osmolytes, and thereby optimize the plant for absorption of sunlight and production of energy by photosynthesis...
  72. ncbi Vacuolar degradation of two integral plasma membrane proteins, AtLRR84A and OsSCAMP1, is cargo ubiquitination-independent and prevacuolar compartment-mediated in plant cells
    Yi Cai
    School of Life Sciences, Centre for Cell and Developmental Biology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China
    Traffic 13:1023-40. 2012
    ..Thus, cargo ubiquitination-independent and PVC-mediated degradation of PM proteins in the vacuole is functionally operated in plant cells...
  73. ncbi Pexophagy: the selective autophagy of peroxisomes
    William A Dunn
    Department of Anatomy and Cell Biology, University of Florida College of Medicine, Gainesville, Florida, USA
    Autophagy 1:75-83. 2005
    ..Utilizing several genetic screens, we have identified a number of genes that are essential for pexophagy. In this review, we will summarize our current knowledge of the molecular events of pexophagy...
  74. pmc Phosphorylation of a membrane curvature-sensing motif switches function of the HOPS subunit Vps41 in membrane tethering
    Margarita Cabrera
    Department of Biology Chemistry, Biochemistry Section, University of Osnabruck, 49076 Osnabruck, Germany
    J Cell Biol 191:845-59. 2010
    ..This multifunctional tethering factor thus discriminates between trafficking routes by switching from a curvature-sensing to a coat recognition mode upon phosphorylation...
  75. ncbi Macropinocytosis is the endocytic pathway that mediates macrophage foam cell formation with native low density lipoprotein
    Howard S Kruth
    Section of Experimental Atherosclerosis, NHLBI, National Institutes of Health, Bethesda, Maryland 20892 1422, USA
    J Biol Chem 280:2352-60. 2005
    ..1 microm diameter) or by macropinocytosis within vacuoles ( approximately 0.5-5.0 microm) named macropinosomes...
  76. ncbi Defined subunit arrangement and rab interactions are required for functionality of the HOPS tethering complex
    Clemens W Ostrowicz
    Department of Biology, Biochemistry Section, University of Osnabruck, Barbarastrasse 13, 49076 Osnabruck, Germany
    Traffic 11:1334-46. 2010
    ..As HOPS Vps39 is also found at endosomes, our data thus suggest that these tethering complexes follow defined but distinct assembly pathways, and may undergo transition by simple subunit interchange...
  77. pmc Genesis of and trafficking to the Maurer's clefts of Plasmodium falciparum-infected erythrocytes
    Cornelia Spycher
    Department of Medical Parasitology and Infection Biology, Swiss Tropical Institute, Socinstrasse 57, CH 4002 Basel, Switzerland
    Mol Cell Biol 26:4074-85. 2006
    ..The data suggest that the Maurer's clefts bud from the parasitophorous vacuole membrane and diffuse within the erythrocyte cytoplasm before taking up residence at the cell periphery...
  78. pmc Tonoplast-localized Abc2 transporter mediates phytochelatin accumulation in vacuoles and confers cadmium tolerance
    David G Mendoza-Cózatl
    Division of Biological Sciences, Cell and Developmental Biology Section, University of California, San Diego, La Jolla, California 92093, USA
    J Biol Chem 285:40416-26. 2010
    ..mediate tolerance to heavy metals in plants and some fungi by sequestering phytochelatin-metal complexes into vacuoles. To date, only Schizosaccharomyces pombe Hmt1 has been described as a phytochelatin transporter and attempts to ..
  79. pmc Rice two-pore K+ channels are expressed in different types of vacuoles
    Stanislav Isayenkov
    Biology Department Area 9, University of York, York YO105DD, United Kingdom
    Plant Cell 23:756-68. 2011
    ..Plants also contain smaller protein storage vacuoles (PSVs) that contain membrane transporters...
  80. ncbi Autophagy delivers misfolded secretory proteins accumulated in endoplasmic reticulum to vacuoles in the filamentous fungus Aspergillus oryzae
    Shinya Kimura
    Department of Biotechnology, The University of Tokyo, Bunkyo ku, Tokyo 113 8657, Japan
    Biochem Biophys Res Commun 406:464-70. 2011
    ..secretory protein α-amylase AmyB fused with mDsRed that had accumulated in the ER was subsequently delivered to vacuoles, whereas wild-type AmyB-mDsRed was predominantly located at cell walls and septa...
  81. ncbi Pexophagy: autophagic degradation of peroxisomes
    Yasuyoshi Sakai
    Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake, Kyoto 606 8502, Japan
    Biochim Biophys Acta 1763:1767-75. 2006
    ..During micropexophagy, a cluster of peroxisomes is enclosed by vacuolar membrane protrusions and/or segmented vacuoles as well as a newly formed membrane structure, the micropexophagy-specific membrane apparatus (MIPA), which ..
  82. pmc Localization of protein kinase C epsilon to macrophage vacuoles perforated by Listeria monocytogenes cytolysin
    Lee M Shaughnessy
    Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109, USA
    Cell Microbiol 9:1695-704. 2007
    Three proteins secreted by Listeria monocytogenes facilitate escape from macrophage vacuoles: the cholesterol-dependent cytolysin listeriolysin O (LLO), a phosphoinositide-specific phospholipase C (PI-PLC) and a broad-range phospholipase ..
  83. ncbi Autophagy induction favours the generation and maturation of the Coxiella-replicative vacuoles
    Maximiliano G Gutierrez
    Instituto de Histologia y Embriologia, Facultad de Ciencias Medicas, Universidad Nacional de Cuyo CONICET, Mendoza, 5500, Argentina
    Cell Microbiol 7:981-93. 2005
    ..man is a Gram-negative gamma-proteobacterium which multiplies in large, acidified, hydrolase-rich and fusogenic vacuoles with phagolysosomal-like characteristics. We reported previously that C...
  84. ncbi The autophagic pathway is actively modulated by phase II Coxiella burnetii to efficiently replicate in the host cell
    Patricia S Romano
    Laboratorio de Biologia Celular y Molecular, IHEM CONICET, Facultad de Ciencias Medicas, Universidad Nacional de Cuyo Mendoza, 5500, Argentina
    Cell Microbiol 9:891-909. 2007
    The etiologic agent of Q fever Coxiella burnetii, is an intracellular obligate parasite that develops large vacuoles with phagolysosomal characteristics, containing multiple replicating bacteria. We have previously shown that Phase II C...
  85. ncbi Phosphatidylinositol 3-kinase encoded by yeast VPS34 gene essential for protein sorting
    P V Schu
    Division of Cellular and Molecular Medicine, University of California, San Diego, School of Medicine
    Science 260:88-91. 1993
    ..VPS34 encodes a yeast PI 3-kinase, and this enzyme appears to regulate intracellular protein trafficking decisions...
  86. pmc The yeast lysosome-like vacuole: endpoint and crossroads
    Sheena Claire Li
    Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, 750 East Adams Street, Syracuse, NY 13210, USA
    Biochim Biophys Acta 1793:650-63. 2009
    Fungal vacuoles are acidic organelles with degradative and storage capabilities that have many similarities to mammalian lysosomes and plant vacuoles...
  87. pmc Phosphatidylinositol 4-phosphate controls both membrane recruitment and a regulatory switch of the Rab GEF Sec2p
    Emi Mizuno-Yamasaki
    Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA 92093, USA
    Dev Cell 18:828-40. 2010
    ..In this way, the regulation of PI4P levels may switch Sec2p/Sec4p function during vesicle maturation, from a Rab GEF recruitment cascade involving Ypt32p to an effector positive feedback loop involving Sec15p...
  88. pmc The gamma interferon (IFN-gamma)-inducible GTP-binding protein IGTP is necessary for toxoplasma vacuolar disruption and induces parasite egression in IFN-gamma-stimulated astrocytes
    T Melzer
    Dept of Microbiology, Montana State University, Bozeman, MT 59717, USA
    Infect Immun 76:4883-94. 2008
    ..Fusion of the ER with the PV, releasing calcium into the vacuole, may also be the mechanism by which intravacuolar parasite movement and IFN-gamma-induced parasite egression occur...
  89. pmc Recruitment of Atg9 to the preautophagosomal structure by Atg11 is essential for selective autophagy in budding yeast
    Congcong He
    Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA
    J Cell Biol 175:925-35. 2006
    ..We propose that a pool of Atg11 mediates the anterograde transport of Atg9 to the PAS that is dependent on the actin cytoskeleton during yeast vegetative growth...
  90. pmc Inhibition of the PtdIns(5) kinase PIKfyve disrupts intracellular replication of Salmonella
    Markus C Kerr
    Institute for Molecular Bioscience and ARC Centre of Excellence in Bioinformatics, The University of Queensland, St Lucia, Queensland, Australia
    EMBO J 29:1331-47. 2010
    ..Finally, although inhibition of PIKfyve in macrophages did inhibit Salmonella replication, it also appears to disrupt the macrophage's bactericidal response...
  91. ncbi Recognition of bacteria in the cytosol of Mammalian cells by the ubiquitin system
    Andrew J Perrin
    Infection, Immunity, Injury, and Repair Program, Hospital for Sick Children, 555 University Avenue, Toronto, Ontario M5G 1X8, Canada
    Curr Biol 14:806-11. 2004
    ..We show that some wild-type bacteria escape invasion vacuoles and are released into the cytosol...
  92. pmc Atg22 recycles amino acids to link the degradative and recycling functions of autophagy
    Zhifen Yang
    Life Sciences Institute, Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA
    Mol Biol Cell 17:5094-104. 2006
    ..We propose a "recycling" model that includes the efflux of macromolecules from the lysosome/vacuole as the final step of autophagy...
  93. pmc Leading a sheltered life: intracellular pathogens and maintenance of vacuolar compartments
    Yadunanda Kumar
    Center for Microbial Pathogenesis, Department of Molecular Genetics and Microbiology, Duke University, Durham, NC 27710, USA
    Cell Host Microbe 5:593-601. 2009
    ..and establish a replicative vacuole are well described, but how the integrity and stability of these parasitic vacuoles are maintained is poorly understood...
  94. ncbi The Mon1-Ccz1 complex is the GEF of the late endosomal Rab7 homolog Ypt7
    Mirjana Nordmann
    Department of Biology Chemistry, University of Osnabruck, Germany
    Curr Biol 20:1654-9. 2010
    ..The Mon1-Ccz1 complex, but neither protein alone, counteracts GAP function in vivo, rescues in vitro fusion of vacuoles carrying Ypt7-GDP, and promotes nucleotide exchange on Ypt7 independently of Vps39/HOPS...
  95. ncbi The Legionella pneumophila effector protein DrrA is a Rab1 guanine nucleotide-exchange factor
    Takahiro Murata
    Section of Microbial Pathogenesis, Yale University School of Medicine, Boyer Center for Molecular Medicine, 295 Congress Avenue, New Haven, CT 06536, USA
    Nat Cell Biol 8:971-7. 2006
    ....
  96. pmc Direct evidence of active and rapid nuclear degradation triggered by vacuole rupture during programmed cell death in Zinnia
    K Obara
    Department of Biological Sciences, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113 0033, Japan
    Plant Physiol 125:615-26. 2001
    ..These results demonstrate that TE differentiation involves a unique type of PCD in which active and rapid nuclear degradation is triggered by vacuole rupture...
  97. ncbi Sorting of proteins to storage vacuoles: how many mechanisms?
    Alessandro Vitale
    Istituto di Biologia e Biotecnologia Agraria, CNR, 20133 Milano, Italy
    Trends Plant Sci 10:316-23. 2005
    b>Vacuoles receive their proteins through the secretory pathway, this requires protein sorting signals and molecular machineries that, until recently, have been believed to be markedly distinct for lytic and storage vacuoles...
  98. ncbi Cytoplasmic bacteria can be targets for autophagy
    Kathryn A Rich
    Ahmanson Advanced Electron Microscopy and Imaging Center, House Ear Institute, and Department of Pathology, Keck School of Medicine, USC, Los Angeles, CA, USA
    Cell Microbiol 5:455-68. 2003
    ..The process involves the sequestration of cytoplasm and organelles into double-membrane autophagic vacuoles for subsequent breakdown within lysosomes...
  99. pmc Structure and distribution of organelles and cellular location of calcium transporters in Neurospora crassa
    Barry J Bowman
    Department of Molecular, Cell, and Developmental Biology, University of California, Santa Cruz, CA 95064, USA
    Eukaryot Cell 8:1845-55. 2009
    ..tubules and vesicles near the hyphal tip, while in more distal regions they were in large and small spherical vacuoles. Mitochondria, visualized with tagged ARG-4, were abundant in all regions of the hyphae...
  100. pmc Arabidopsis V-ATPase activity at the tonoplast is required for efficient nutrient storage but not for sodium accumulation
    Melanie Krebs
    Heidelberg Institute for Plant Sciences, University of Heidelberg, 69120 Heidelberg, Germany
    Proc Natl Acad Sci U S A 107:3251-6. 2010
    ....
  101. pmc The Arabidopsis cax1 mutant exhibits impaired ion homeostasis, development, and hormonal responses and reveals interplay among vacuolar transporters
    Ning Hui Cheng
    United States Department of Agriculture Agricultural Research Service Children s Nutrition Research Center, Baylor College of Medicine, Houston, Texas 77030, USA
    Plant Cell 15:347-64. 2003
    ..We propose that CAX1 regulates myriad plant processes and discuss the observed phenotypes with regard to the compensatory alterations in other transporters...

Research Grants67

  1. Role of Endocytosis and Vesicular Trafficking in Podocyte Health and Filter Integ
    Puneet Garg; Fiscal Year: 2012
    ..As anticipated, mice deleted of vps34 in a podocyte specific manner develop early proteinuria and large vacuoles are visible in the podocytes...
  2. Validation of Novel Infertility Biomarker
    PETER SUTOVSKY SUTOVSKY; Fiscal Year: 2012
    ..we will correlate sperm chromatin structure, likely affected by the presence of SPTRX3-containing nuclear vacuoles, with flow cytometric SPTRX3 data...
  3. Enzymology of Chlamydia Pathogenesis
    Dewey G McCafferty; Fiscal Year: 2013
    ..trachomatis persists within parasitophorous inclusion vacuoles. During infection, C...
  4. Liver Cell Membrane Protein-Expression and Function
    Allan W Wolkoff; Fiscal Year: 2012
    ..of trafficking steps in endocytosis mutants (Project 3), and mechanisms of trafficking to autophagic vacuoles and lysosomes (Project 4)...
  5. Phagosomal Signals Shape Inflammatory Responses to Borrelia Burgdorferi
    Juan C Salazar; Fiscal Year: 2010
    ..In Aim 2 we will determine if components of internalized, degraded Borrelia burgdorferi leak out of phagocytic vacuoles to engage cytosolic PRRs...
  6. V-ATPase H+ PUMP REGULATION IN FUEL ENERGY SELECTION
    Karlett J Parra; Fiscal Year: 2013
    ..Located at the membrane of lysosomes, vacuoles and endosomes, V-ATPases sustain the acidic luminal pH needed for protein sorting and degradation;and for entry ..
  7. Molecular Mechanisms of Host Function Exploitation by Type IV effectors of Legion
    Zhao Qing Luo; Fiscal Year: 2013
    ..Further, acquisition of endoplasmic reticulum proteins such as calnexin by vacuoles harboring the sidJ deletion mutant was delayed...
  8. Activation of rapamycin-sensitive TORC1 by endomembrane amino acid transporters
    MARIA E CARDENAS-CORONA; Fiscal Year: 2013
    ..Intriguingly, TORC1 and the EGOC colocalize to endomembranes including those of endosomes and vacuoles, along with vacuolar amino acid transporters...
  9. How does misregulation of PI3,5P2 signaling lead to neurodegeneration?
    Lois S Weisman; Fiscal Year: 2013
    ..Affected neurons in both the brain and the peripheral nervous system develop large vacuoles that arise from endosomes...
  10. New Thoughts on Parietal Epithelial Cells
    Stuart Shankland; Fiscal Year: 2010
    ..We will also test the hypothesis that PECs normally take up filtered albumin into vacuoles for lysozymal degradation...
  11. Autophagic Lysosomal Pathway and Glaucoma
    PALOMA LITON; Fiscal Year: 2013
    ..pathway, including: (1) Increased lysosomal mass and lysosomal enzymes protein content, (2) increased autophagic vacuoles content, (3) upregulated levels of LC3-II, (4) accumulation of intralysosomal oxidized material and damaged ..
  12. Analysis of the Coxiella burnetii Type IV Secretion System During Infection
    Edward I Shaw; Fiscal Year: 2012
    ..After infecting the host cell, C. burnetii replicates in vacuoles that retain many of the features of mature phagolysosomes. The molecular mechanisms used by C...
  13. Fifth World Congress of Leishmaniasis 2013
    Lynn Soong; Fiscal Year: 2013
    ..The active replication and long-term persistence of parasites within specialized vacuoles of macrophages and dendritic cells can lead to dysfunction of host innate and adaptive immunity...
  14. INTRACELLULAR LIFESTYLE OF PSEUDOMONAS AERUGINOSA
    SUZANNE MARIANE JANETE FLEISZIG; Fiscal Year: 2013
    ..aeruginosa. T3SS mutants fail to form blebs and instead localize to perinuclear vacuoles. In contrast to wild type bacteria, T3SS mutants (retain competency for invasion) lose viability after entering ..
  15. Bacterial Survival in the Mammalian Urothelium
    JOSEPH PALERMO; Fiscal Year: 2010
    ..Moreover, the IBCs are present in the cytoplasm of the superficial umbrella cells, not membrane bound vacuoles as seen in cancer cell monolayers...
  16. A novel role for PARP1 in regulation of autophagy
    Gen Sheng Wu; Fiscal Year: 2013
    ..contributions to the induction of autophagy, a process whereby cytosol and damaged organelles are encapsulated in vacuoles termed autophagosomes that fuse with lysosomes, leading to degradation of the sequestered cargo...
  17. Role of the antiviral ribonuclease, RNase-L, in the host antibacterial response
    Bret A Hassel; Fiscal Year: 2012
    ..with the altered CatE expression in RNase-L-/- macrophages, the endolysosome-mediated clearance of phagocytic vacuoles was disrupted following bacterial infection...
  18. Apoptosis and Necrosis in Pancreatitis
    Anna S Gukovskaya; Fiscal Year: 2012
    ..A recognized feature of this disease is the accumulation of vacuoles in acinar cells...
  19. Mitochondrial Dysfunction, Permeability Transition Pore, and Acute Pancreatitis
    Anna S Gukovskaya; Fiscal Year: 2013
    ..pancreatic acinar cell, mitochondria, permeability transition pore, lysosomes, cathepsin, autophagy, trypsin, vacuoles. Aims: Acute pancreatitis is a potentially fatal disease of exocrine pancreas, the pathogenesis of which remains ..
  20. Development of swallow evoked potentials as a novel tool to investigate swallowin
    Teresa E Lever; Fiscal Year: 2012
    ..that are activated during swallowing;these regions also will be investigated for evidence of histopathology (vacuoles)...
  21. Autophagic Clearance of Aberrant Tau: Biochemical and Therapeutic Implications
    Karen E Duff; Fiscal Year: 2013
    ..animal models to test the impact of dysfunction in a particular pathway (abnormal transport of autophagic vacuoles leading to failure of autophagic flux) and its impact on tauopathy, and whether compounds that activate A-LS or ..
  22. Cellular Physiology of the Aqueous Outflow Pathway
    Haiyan Gong; Fiscal Year: 2013
    ..Giant vacuoles and pores, which are characteristic features of the endothelial cells of SC, have long been suspected to play a ..
  23. The Protein Degradation Pathway after Brain Ischemia
    Bingren Hu; Fiscal Year: 2013
    ..Our latest studies show that accumulation of: (i) autophagy vacuoles (AVs), (ii) protein aggregates, and (iii) aberrant organelles are the most prominent early ultrastructural ..
  24. Uncovering mechanisms controlling notochord vacuole and spine morphogenesis
    Michel Bagnat; Fiscal Year: 2013
    ..These fluid-filled vacuoles have been described in every vertebrate embryo studied including fish, amphibians, birds, and mammals...
  25. Role of Septins in Organelle Transfer
    Alan Michael Tartakoff; Fiscal Year: 2013
    ..membrane proteins of the cell cortex and nuclear envelope, and is responsible for the observation that parental vacuoles do not exchange components with each other in early zygotes...
  26. Molecular characteristics of the apical recycling system
    JAMES RICHARD GOLDENRING; Fiscal Year: 2013
    ..brush border and the presence of intracellular inclusions that appear to represent intracellular apical membrane vacuoles. All of the patients from the Navajo tribe have a single point mutation that inactivates proper motor function...
  27. The plant-like vacuole of Toxoplasma gondii
    Silvia N Moreno; Fiscal Year: 2013
    ..Physiological studies further revealed similarities to plant vacuoles such as the presence of a V-H+ATPase, Na? and Ca2? antiporters, and calcium storage...
  28. Structural Analysis of Golgi Trafficking Proteins
    Frederick M Hughson; Fiscal Year: 2013
    ..Finally, membrane fusion at late endosomes and lysosomes/vacuoles depends on the HOPS complex...
  29. Flow Cytometry HTS of Small Molecules that Regulate V-ATPase Proton Transport in
    Karlett J Parra; Fiscal Year: 2012
    ..For the Secondary Assays the compounds are added to cells stained with BCECF trapped in the vacuoles. V-ATPase inhibitors will increase the vacuolar pH and enhance the fluorescence intensity of BCECF...
  30. Molecular basis for skeletal muscle pathophysiology in Pompe's disease
    Jeffrey E Pessin; Fiscal Year: 2013
    ..type II muscle fibers (white muscle) are primarily affected in Pompe's disease and accumulate autophagic vacuoles whereas oxidative type I muscle fibers (red) are much less unaffected...
  31. Mechanisms of age-related RPE dysfunction and CNV
    Jiyang Cai; Fiscal Year: 2013
    ..We found that Nrf2-deficient RPE cells had increased accumulation of autophagic vacuoles and lipofuscin in vivo, indicating less efficient degradation by lysosome...
  32. SB Transposons for Gene Therapy
    Perry B Hackett; Fiscal Year: 2013
    ..GUSB) and a-L-iduronidase (IDUA) encoding genes to mouse liver and thereby correct accumulation of storage vacuoles in non-neural tissues of MPS I mice...
  33. HBV replication and carcinogenesis
    J H James Ou; Fiscal Year: 2013
    ..We will also investigate how autophagy enhances HBV DNA replication. We will determine whether autophagic vacuoles serve as the platform for HBV DNA replication and whether autophagy affects cellular factors that regulate HBV ..
  34. MOLECULAR GENETICS OF CNS DEVELOPMENT
    KAI G ZINN; Fiscal Year: 2013
    ..In the double mutant embryos, apical proteins accumulate in large intracellular vacuoles ("bubbles"). Basolateral proteins are localized normally...
  35. Interferon gamma-mediated restriction of Shigella flexneri replication
    Michael N Starnbach; Fiscal Year: 2013
    ..resistance mechanisms that have been described are targeted to microbes that replicate in pathogen containing vacuoles (PCVs)...
  36. Radiosensitization via Targeting Cell Death
    Bo Lu; Fiscal Year: 2010
    ..Autophagy, a highly conserved self-elimination mechanism that utilizes lysosome/vacuoles for cellular degradation is a backup cell death pathway when apoptosis is blocked...
  37. Role of Host Lipids in Salmonella Replication Within Hemophagocytic Macrophages
    TONI NAGY; Fiscal Year: 2013
    ..One way HM?s differ from resting or activated macrophages is that these cells accumulate vacuoles that appear to contain fatty acids. Previous studies have suggested the importance of S...
  38. Targeting of Interferon-induced host proteins to Legionella-containing vacuoles
    JOERN COERS; Fiscal Year: 2013
    ..is the induction of cell-autonomous resistance towards bacterial pathogens residing and replicating within vacuoles, including Mycobacterium tuberculosis and Legionella pneumophila...
  39. Human iPSC-Derived Cardiomyocyte Model for Danon Disease and Heart Failure
    Cynthia Perry; Fiscal Year: 2012
    ..The hallmark of this disorder is the accumulation of large autophagic vacuoles due to the failure of autophagosome-lysosome fusion...
  40. How bacteria corrupt the host vesicular trafficking:Role of SNARE-like Proteins
    FABIENNE MICHELLE PAUMET; Fiscal Year: 2012
    ..Results will contribute to a better understanding of how parasite-containing vacuoles bypass lysosomal degradation and will shed light on the mechanism of parasitic intracellular survival...
  41. Tubovesicular traffic induced in red cells in plasmodia
    Kasturi Haldar; Fiscal Year: 2012
    ..The long term aim of this proposal is to identify and characterize the mechanisms by which endocytic vacuoles in erythrocytes can be induced by malaria parasites...
  42. Targeting Host and Apicomplexan Isoprenoid Pathways
    Silvia N Moreno; Fiscal Year: 2013
    ..T. gondii resides intracellularly within parasitophorous vacuoles, and reactivation and dissemination of infection to different organs may occur following immune suppression...
  43. IBMPFD MUTATIONS IMPAIR UPS FUNCTION
    Conrad C Weihl; Fiscal Year: 2013
    ..There is no effective treatment. IBM muscle has characteristic "rimmed vacuoles" and eosinophilic inclusions...
  44. Mechanisms of intracellular pathogen dissemination
    HERVE F AGAISSE; Fiscal Year: 2013
    ..These protrusions are resolved in the receiving cells into double membrane vacuoles, from which the pathogens escape by producing virulence factors that disrupt the integrity of eukaryotic ..
  45. Modulation of Host Vesicle Trafficking by Legionella pneumophila
    Zhao Qing Luo; Fiscal Year: 2013
    ..one of such effectors called SidJ plays an important role in the biogenesis of replicative Legionella-containing vacuoles (LCVs) and loss of SidJ results in a temporal delay in the recruitment of ER proteins to the LCVs...
  46. Translational Studies in VCP Inclusion Body Myopathy with Paget Disease of Bone
    Virginia Kimonis; Fiscal Year: 2009
    ..decreased muscle strength, disrupted muscle fibers, TDP-43 and ubiquitin positive inclusion bodies and vacuoles in muscle, cognitive deficits and Paget-like bone lesions...
  47. Bacterial inhibitors of eukaryotic membrane fusion
    VINCENT JOSEPH STARAI; Fiscal Year: 2013
    ..my laboratory has employed a powerful biochemical model of eukaryotic membrane fusion, the homotypic fusion of vacuoles from the yeast Saccharomyces cerevisiae (Sce), to begin the characterization of a protein from Lpn, LegC3, that ..
  48. Intracellular Membrane Fusion Mediated by SNARE Proteins
    Christopher Peters; Fiscal Year: 2013
    ..In order to unravel the precise fusion mechanism the yeast vacuolar fusion system is employed. Yeast vacuoles are purified in a large-scale isolation procedure and reconstituted in an in vitro system...
  49. Role of ExoY in Corneal Eye Infection
    Victoria Hritonenko; Fiscal Year: 2012
    ..aeruginosa within corneal epithelial cells involving escape from perinuclear vacuoles and trafficking to infection-induced bleb-niches...
  50. PROTEIN COMPARTMENTATION
    WILLIAM TOBEY WICKNER; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): We have developed yeast vacuoles as a paradigm of membrane fusion in endocytic and exocytic traffic...
  51. Cisplatin and Mechanisms of Long-term Male Infertility
    JOHN RICHBURG; Fiscal Year: 2007
    ..Histopathology of testes from cisplatin-treated mice revealed large Sertoli cell vacuoles, apical sloughing, shedding of cellular material into the lumen and loss of germ cells by apoptosis, which are ..
  52. EP164 coordinates arf6- and Rab-dependent membrane traffic in epithelial cells
    David E Hokanson; Fiscal Year: 2010
    ..binds directly to active Arf6-GTP and EPI64's over- expression induces an accumulation of actin-coated vacuoles likely driven by the over production of PIP2 via PI4P5K activation...
  53. GENETIC ANALYSIS OF LEGIONELLA PHAGOSOME TRAFFICKING
    Craig R Roy; Fiscal Year: 2013
    ..of these proteins are regulated spatially and temporally during infection to coordinate the cycling of Rab1 on vacuoles containing Legionella, determine how Rab1 activation on the vacuole containing Legionella can promote the ..
  54. Structure of Chlamydia-Specific Host Cell Vacuoles
    Richard S Stephens; Fiscal Year: 2010
    ..This research will establish the mechanism of microbial survival in cells that will serve as targets for prevention and control of chlamydial infection. ..
  55. Coordination of membrane fission and fusion machineries to control size and copy
    ANDREAS R MAYER; Fiscal Year: 2010
    ..the elementary processes of membrane fission and fusion coordinated in order to control these parameters? Yeast vacuoles (lysosomes) can serve as an excellent model to study this question: Vacuoles change copy number and size in the ..
  56. AUTOPHAGY IN GLIA AND NEURONS
    William Dunn; Fiscal Year: 1993
    ..Autophagy is a normal process whereby cellular components (ie, mitochondria) are sequestered within RER-derived vacuoles and ultimately degraded following fusion of these vacuoles with lysosomes...
  57. Neurotoxicity of NMDA Receptor Antagonists
    Frank Sharp; Fiscal Year: 2005
    ..The drugs also injure rodent limbic cortex, killing some neurons and injuring others that have cytoplasmic vacuoles and express HSP7O and HO-i heat shock proteins...
  58. Pathophysiology of Lysosomal Free Sialic Acid Storage Disorders
    Richard J Reimer; Fiscal Year: 2010
    ..Enlarged cytoplasmic vacuoles filled with the amino sugar sialic acid define the cellular pathology and a defect in transport of sialic acid ..
  59. Quantitative characterization of vacuole size in budding yeast
    YEE HUNG MARK CHAN; Fiscal Year: 2011
    ..I will use three-dimensional fluorescence imaging to visualize yeast vacuoles using exogenous or genetically-encoded protein markers...
  60. EFFECT OF ALCOHOL ON PANCREATIC SECRETION
    Jorge Valenzuela; Fiscal Year: 1990
    ..As a result, secretory enzymes either fuse with lysosomal enzymes in vacuoles (crynophagia) or are released to the cytoplasm and interstitium...
  61. Mechanisms controlling amino acid signaling to mTOR and lysosome fission.
    MICHAEL H OVERHOLTZER; Fiscal Year: 2013
    ..of mRNA translation and autophagy, is required for a program of phagosome fission that shrinks large lysosomal vacuoles as internalized cargo is degraded, which reminiscent of the recently described autophagic lysosome reformation (..
  62. INTERMEDIARY METABOLISM OF LEISHMANIA
    JACOB BLUM; Fiscal Year: 1993
    ..Electron probe X-ray microanalysis shows the presence of dense vacuoles rich in phosphorous and magnesium...
  63. An in vivo Gene Deletion System for Analyzing Temporal Requirement of the Dot/Icm
    Zhao Qing Luo; Fiscal Year: 2009
    ..protein substrates into host cells and bacterial multiplication will be analyzed by the formation of replicative vacuoles as well as by total bacterial counts...
  64. VACUOLE FUNCTION IN NEUROSPORA
    Richard Weiss; Fiscal Year: 1993
    ..Arginine, ornithine and a number of other amino acids are sequestered in the vacuoles in this organism. A specific arginine carrier has been identified in the tonoplast...
  65. Germination of Bacillus anthracis Spores in Macrophages
    Daoguo Zhou; Fiscal Year: 2004
    ..Results from our study will define the compartment of the vacuoles required for B. anthracis germination, and the macrophage-specific germination-deficient B...
  66. Mechanism of neurodegeneration in dystonia musculorum
    RONALD LIEM; Fiscal Year: 2007
    ..root ganglia of dt mice are considerably smaller in size than those of wild type mice with organelles and empty vacuoles accumulating within sensory axons...
  67. BACTERIAL DEFENSES AGAINST PHAGOCYTE OXIDATIVE KILLING
    John Eaton; Fiscal Year: 1990
    ..such as Leishmania, Legionella, and Mycobacter may survive and replicate within macrophage/monocyte phagocytic vacuoles, thereby gaining protection from recognition and destruction by host neutrophils, antibodies, and complement...