cytoplasmic vesicles

Summary

Summary: Membrane-limited structures derived from the plasma membrane or various intracellular membranes which function in storage, transport or metabolism.

Top Publications

  1. ncbi Gram-positive bacteria produce membrane vesicles: proteomics-based characterization of Staphylococcus aureus-derived membrane vesicles
    Eun Young Lee
    Department of Life Science, Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang, Republic of Korea
    Proteomics 9:5425-36. 2009
  2. pmc Tumour-derived exosomes and their role in cancer-associated T-cell signalling defects
    D D Taylor
    Department of Obstetrics, Gynecology and Women s Health, University of Louisville School of Medicine, Louisville, KY 40202, USA
    Br J Cancer 92:305-11. 2005
  3. ncbi Organization of vesicular trafficking in epithelia
    Enrique Rodriguez-Boulan
    Margaret Dyson Vision Research Institute, Weill Medical College of Cornell University, 1300 York Avenue, New York, New York 10021, USA
    Nat Rev Mol Cell Biol 6:233-47. 2005
  4. ncbi Dissecting multiple steps of GLUT4 trafficking and identifying the sites of insulin action
    Li Bai
    Joint Laboratory of Institute of Biophysics and Huazhong University of Science and Technology, National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
    Cell Metab 5:47-57. 2007
  5. ncbi SNAREs and traffic
    Wanjin Hong
    Membrane Biology Laboratory, Institute of Molecular and Cell Biology, Sinapore, Singapore
    Biochim Biophys Acta 1744:493-517. 2005
  6. ncbi Fas ligand-positive membranous vesicles isolated from sera of patients with oral cancer induce apoptosis of activated T lymphocytes
    Jeong Whun Kim
    University of Pittsburgh Cancer Institute, Hillman Cancer Center, 5117 Centre Avenue, Pittsburgh, PA 15213, USA
    Clin Cancer Res 11:1010-20. 2005
  7. ncbi Malignant effusions and immunogenic tumour-derived exosomes
    Fabrice Andre
    Departments of Clinical Biology, Immunology Unit, Institut Gustave Roussy, Villejuif, France
    Lancet 360:295-305. 2002
  8. ncbi The Wnt signalling effector Dishevelled forms dynamic protein assemblies rather than stable associations with cytoplasmic vesicles
    Thomas Schwarz-Romond
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 2QH, UK
    J Cell Sci 118:5269-77. 2005
  9. ncbi Vesicles generated during storage of red cells are rich in the lipid raft marker stomatin
    Ulrich Salzer
    Department of Vascular Biology and Thrombosis Research, Medical University of Vienna, Vienna, Austria
    Transfusion 48:451-62. 2008
  10. pmc PKCepsilon-mediated phosphorylation of vimentin controls integrin recycling and motility
    Johanna Ivaska
    VTT Technical Research Centre for Finland, Medical Biotechnology and University of Turku Centre for Biotechnology, Turku, Finland
    EMBO J 24:3834-45. 2005

Research Grants

  1. REGULATION AND FUNCTION OF STE20-RELATED PROTEIN KINASES
    Melanie H Cobb; Fiscal Year: 2012
  2. Sodium-chloride co-transporter regulation in the kidney
    Alicia A McDonough; Fiscal Year: 2013
  3. Characterization of AQP2 interacting proteins and novel functions of AQP2
    HUA A LU; Fiscal Year: 2010
  4. RASCAL: a new tropism determinant encoded by human cytomegalovirus?
    Laura Hertel; Fiscal Year: 2012
  5. EBV EXPRESSION IN NASOPHARYNGEAL CARCINOMA
    NANCY JOAN RAAB-TRAUB; Fiscal Year: 2013
  6. Acinar Biology and Pancreatic Disease
    Guy E Groblewski; Fiscal Year: 2011
  7. Endothelial SK3 Channel Modulation of EDHF is Estrogen Regulated
    Mike T Lin; Fiscal Year: 2013
  8. Acinar Biology and Pancreatic Disease
    Guy E Groblewski; Fiscal Year: 2013
  9. CHOLESTEROL AND NEURODEGENERATION
    Shutish Patel; Fiscal Year: 2001
  10. BASOPHIL/MAST CELL FUNCTION
    Ann Dvorak; Fiscal Year: 1991

Detail Information

Publications235 found, 100 shown here

  1. ncbi Gram-positive bacteria produce membrane vesicles: proteomics-based characterization of Staphylococcus aureus-derived membrane vesicles
    Eun Young Lee
    Department of Life Science, Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang, Republic of Korea
    Proteomics 9:5425-36. 2009
    ..This information will help us not only to elucidate the biogenesis and functions of MVs, but also to develop therapeutic tools for vaccines, diagnosis, and antibiotics effective against pathogenic strains of Gram-positive bacteria...
  2. pmc Tumour-derived exosomes and their role in cancer-associated T-cell signalling defects
    D D Taylor
    Department of Obstetrics, Gynecology and Women s Health, University of Louisville School of Medicine, Louisville, KY 40202, USA
    Br J Cancer 92:305-11. 2005
    ..These results indicate that, while 'exosomes' express tumour antigens, leading to their proposed utility as tumour vaccines, they also can suppress T-cell signalling molecules and induce apoptosis...
  3. ncbi Organization of vesicular trafficking in epithelia
    Enrique Rodriguez-Boulan
    Margaret Dyson Vision Research Institute, Weill Medical College of Cornell University, 1300 York Avenue, New York, New York 10021, USA
    Nat Rev Mol Cell Biol 6:233-47. 2005
    ..Recent live-cell-imaging studies provide a real-time view of sorting processes in epithelial cells, including key roles for actin, microtubules and motors in the organization of post-Golgi trafficking...
  4. ncbi Dissecting multiple steps of GLUT4 trafficking and identifying the sites of insulin action
    Li Bai
    Joint Laboratory of Institute of Biophysics and Huazhong University of Science and Technology, National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
    Cell Metab 5:47-57. 2007
    ....
  5. ncbi SNAREs and traffic
    Wanjin Hong
    Membrane Biology Laboratory, Institute of Molecular and Cell Biology, Sinapore, Singapore
    Biochim Biophys Acta 1744:493-517. 2005
    ..In the post-genomic era, a fairly complete list of "all" SNAREs in several organisms (including human) can now be made. This review aims to summarize the key properties and the mechanism of action of SNAREs in mammalian cells...
  6. ncbi Fas ligand-positive membranous vesicles isolated from sera of patients with oral cancer induce apoptosis of activated T lymphocytes
    Jeong Whun Kim
    University of Pittsburgh Cancer Institute, Hillman Cancer Center, 5117 Centre Avenue, Pittsburgh, PA 15213, USA
    Clin Cancer Res 11:1010-20. 2005
    ..The present study was done to identify mechanisms responsible for apoptosis of T cells seen in the peripheral circulation of these patients...
  7. ncbi Malignant effusions and immunogenic tumour-derived exosomes
    Fabrice Andre
    Departments of Clinical Biology, Immunology Unit, Institut Gustave Roussy, Villejuif, France
    Lancet 360:295-305. 2002
    ..To assess the role of these exosomes in vivo, we examined malignant effusions for their presence. We also investigated whether these exosomes could induce production of tumour-specific T cells when pulsed with dendritic cells...
  8. ncbi The Wnt signalling effector Dishevelled forms dynamic protein assemblies rather than stable associations with cytoplasmic vesicles
    Thomas Schwarz-Romond
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 2QH, UK
    J Cell Sci 118:5269-77. 2005
    ..These puncta are thought to reflect cytoplasmic vesicles. However, we show here that the mammalian Dishevelled protein Dvl2 does not colocalise with known vesicle ..
  9. ncbi Vesicles generated during storage of red cells are rich in the lipid raft marker stomatin
    Ulrich Salzer
    Department of Vascular Biology and Thrombosis Research, Medical University of Vienna, Vienna, Austria
    Transfusion 48:451-62. 2008
    ..The characterization of the vesicular membrane might hint at the underlying mechanism of the storage-associated changes in general and the vesiculation process in particular...
  10. pmc PKCepsilon-mediated phosphorylation of vimentin controls integrin recycling and motility
    Johanna Ivaska
    VTT Technical Research Centre for Finland, Medical Biotechnology and University of Turku Centre for Biotechnology, Turku, Finland
    EMBO J 24:3834-45. 2005
    ..Our results indicate that PKC-mediated phosphorylation of vimentin is a key process in integrin traffic through the cell...
  11. pmc Tumor-derived microvesicles induce, expand and up-regulate biological activities of human regulatory T cells (Treg)
    Marta Szajnik
    University of Pittsburgh Cancer Institute, Pittsburgh, Pennsylvania, United States of America
    PLoS ONE 5:e11469. 2010
    ..The hypothesis is tested that TMV contribute to induction/expansion/and activation of human Treg...
  12. pmc Exovesicles from human activated dendritic cells fuse with resting dendritic cells, allowing them to present alloantigens
    Carolina Obregon
    Department of Clinical Research, Institute of Anatomy, University of Bern, and Clinic and Policlinic of Pneumology, University Hospital of Bern, Freiburgstr 15, CH 3010 Berne, Switzerland
    Am J Pathol 169:2127-36. 2006
    ..We concluded that, within a few hours from their release, exovesicles may amplify local or distant adaptive immunological response...
  13. ncbi Exosomes: endosomal-derived vesicles shipping extracellular messages
    Benoit Fevrier
    Institut Curie, Research Section, CNRS UMR 144, 26 Rue d Ulm, 75005 Paris, France
    Curr Opin Cell Biol 16:415-21. 2004
    ..The mechanisms involved in exosome secretion and interaction with target cells are as yet unclear. A better understanding of these mechanisms is also essential to determine the link between exosomes and retroviruses...
  14. ncbi Exosomes: a common pathway for a specialized function
    Guillaume van Niel
    Institut Curie, CNRS UMR144, 75248 Paris Cedex, France
    J Biochem 140:13-21. 2006
    ..These findings open up new therapeutic and diagnostic strategies...
  15. ncbi Exosomes--vesicular carriers for intercellular communication
    Mikael Simons
    Max Planck Institute for Experimental Medicine, Gottingen, Germany
    Curr Opin Cell Biol 21:575-81. 2009
    ..Only recently light have been shed on the subcellular compartments and mechanisms involved in their biogenesis and secretion opening new avenues to understand their functions...
  16. pmc Glut4 storage vesicles without Glut4: transcriptional regulation of insulin-dependent vesicular traffic
    Danielle N Gross
    Department of Biochemistry, Boston University School of Medicine, MA 02118, USA
    Mol Cell Biol 24:7151-62. 2004
    ..The differentiated cells possess a large insulin-sensitive vesicular compartment with negligible Glut4, and Glut4 translocation can be reconstituted on expression of this transporter...
  17. ncbi Matrix vesicles in the fibrous cap of atherosclerotic plaque: possible contribution to plaque rupture
    Y V Bobryshev
    Faculty of Medicine, University of New South Wales, Kensington NSW, Australia
    J Cell Mol Med 12:2073-82. 2008
    ..705+/-0.436 versus 5.322+/-0494; P= 0.0474). The findings reinforce a view that the texture of the extracellular matrix in the thinning fibrous cap of atherosclerotic plaque is altered and this might contribute to plaque destabilization...
  18. ncbi In vitro assessment of halobacterial gas vesicles as a Chlamydia vaccine display and delivery system
    Tawanna S Childs
    Department of Microbiology, University of Massachusetts, Amherst, MA 01003, United States
    Vaccine 30:5942-8. 2012
    ..The data suggest that r-GV could be an effective, naturally adjuvanting, time-release antigen delivery system for immunologically relevant Chlamydia vaccine antigens which are readily recognized by human immune sera...
  19. ncbi Epithelial ovarian cancer cells secrete functional Fas ligand
    Vikki M Abrahams
    Department of Obstetrics and Gynecology, Yale University School of Medicine, New Haven Connecticut 06520, USA
    Cancer Res 63:5573-81. 2003
    ....
  20. pmc Syntaxin 6 regulates Glut4 trafficking in 3T3-L1 adipocytes
    H Kumudu I Perera
    The Henry Wellcome Laboratory of Cell Biology, Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, Scotland, United Kingdom
    Mol Biol Cell 14:2946-58. 2003
    ..We speculate that this is at the level of traffic of Glut4 into its unique storage compartment and that syntaxin 16 may be involved...
  21. pmc Tumor vesicle-associated CD147 modulates the angiogenic capability of endothelial cells
    Danilo Millimaggi
    Department of Experimental Medicine, L Aquila University, L Aquila, Italy
    Neoplasia 9:349-57. 2007
    ..We therefore conclude that vesicles shed by ovarian cancer cells may induce proangiogenic activities of HUVECs by a CD147-mediated mechanism...
  22. ncbi Class C Vps protein complex regulates vacuolar SNARE pairing and is required for vesicle docking/fusion
    T K Sato
    Division of Cellular and Molecular Medicine and Howard Hughes Medical Institute, University of California at San Diego School of Medicine, La Jolla 92093, USA
    Mol Cell 6:661-71. 2000
    ..Therefore, we propose that the C-Vps complex associates with unpaired (activated) Vam3 to mediate the assembly of trans-SNARE complexes during both vesicle docking/fusion and vacuole-to-vacuole fusion...
  23. ncbi RBC-derived vesicles during storage: ultrastructure, protein composition, oxidation, and signaling components
    Anastasios G Kriebardis
    Department of Cell Biology and Biophysics, Faculty of Biology, University of Athens, Panepistimiopolis, Greece
    Transfusion 48:1943-53. 2008
    ..The vesiculation of the RBCs is a part of the storage lesion. The protein composition of the vesicles generated during storage of banked RBCs has not been studied in detail...
  24. pmc Exosome function: from tumor immunology to pathogen biology
    Jeffrey S Schorey
    Center for Global Health and Infectious Diseases, Department of Biological Sciences, University of Notre Dame, Notre Dame, IN 46556, USA
    Traffic 9:871-81. 2008
    ..In this review, we summarize our understanding of exosome biogenesis but focus primarily on new insights into exosome function. We also discuss their possible use as disease biomarkers and vaccine candidates...
  25. ncbi Munc18c interaction with syntaxin 4 monomers and SNARE complex intermediates in GLUT4 vesicle trafficking
    Matthew D'Andrea-Merrins
    Department of Molecular and Integrative Physiology, Life Sciences Institute, University of Michigan, Ann Arbor 48109 0622, USA
    J Biol Chem 282:16553-66. 2007
    ..Therefore, in contradiction to previous models, our data indicates that the conformational "opening" of syntaxin 4 rather than the dissociation of Munc18c is the critical event required for GLUT4 vesicle docking...
  26. pmc Proteomic analysis of GLUT4 storage vesicles reveals LRP1 to be an important vesicle component and target of insulin signaling
    Mark P Jedrychowski
    Department of Biochemistry, Boston University Medical School, Boston, Massachusetts 02118, USA
    J Biol Chem 285:104-14. 2010
    ..Furthermore, adipose-specific LRP1 knock-out mice also exhibit decreased GLUT4 expression. These findings suggest LRP1 is an important component of GSVs, and its expression is needed for the formation of fully functional GSVs...
  27. pmc Cryptococcus neoformans cryoultramicrotomy and vesicle fractionation reveals an intimate association between membrane lipids and glucuronoxylomannan
    Debora L Oliveira
    Laboratorio de Estudos Integrados em Bioquimica Microbiana, Instituto de Microbiologia Professor Paulo de Goes, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941590, Brazil
    Fungal Genet Biol 46:956-63. 2009
    ..These results indicate an intimate association of GXM and lipids in both intracellular and extracellular spaces consistent with polysaccharide synthesis and transport in membrane-associated structures...
  28. ncbi Tumour-released exosomes and their implications in cancer immunity
    M Iero
    Unit of Immunotherapy of Human Tumours, Fondazione IRCCS Istituto Nazionale Tumori, Via Venezian 1, Milan, Italy
    Cell Death Differ 15:80-8. 2008
    ..Hence, their secretion by neoplastic cells may in the future become a novel pathway to target for therapeutic intervention in cancer patients...
  29. pmc Targeting of Tsc13p to nucleus-vacuole junctions: a role for very-long-chain fatty acids in the biogenesis of microautophagic vesicles
    Erik Kvam
    Department of Biology, University of Rochester, Rochester, NY 14627, USA
    Mol Biol Cell 16:3987-98. 2005
    ..These results indicate that Nvj1p retains and compartmentalizes Tsc13p at NV junctions and that VLCFAs contribute to the normal biogenesis of trilaminar PMN structures in yeast...
  30. pmc The essentials of protein import in the degenerate mitochondrion of Entamoeba histolytica
    Pavel Dolezal
    Department of Biochemistry and Molecular Biology, Monash University, Clayton Campus, Melbourne, Victoria, Australia
    PLoS Pathog 6:e1000812. 2010
    ..histolytica...
  31. ncbi Microparticles as regulators of inflammation: novel players of cellular crosstalk in the rheumatic diseases
    Jörg H W Distler
    Institute for Clinical Immunology and Rheumatology, Dept of Internal Medicine III, University of Erlangen Nuremberg, Krankenhausstrasse 12, D 91054 Erlangen, Germany
    Arthritis Rheum 52:3337-48. 2005
  32. ncbi Membrane vesicles shed by oligodendroglioma cells induce neuronal apoptosis
    Stefania D'Agostino
    Dipartimento di Biologia Cellulare e dello Sviluppo, Universita di Palermo, I 90128 Palermo, Italy
    Int J Oncol 29:1075-85. 2006
    ..We therefore conclude that vesicles shed by G26/24 cells induce neuronal apoptosis at least partially by a Fas-L mediated mechanism...
  33. ncbi Concentration of GPI-anchored proteins upon ER exit in yeast
    Guillaume A Castillon
    Department of Biochemistry, University of Geneva, Sciences II, 30 Quai Ernest Ansermet, CH 1211 Geneva, Switzerland
    Traffic 10:186-200. 2009
    ..Additionally, we have found that GPI-anchored protein ER exit is controlled by the p24 family member Emp24p, whereas ER export of most transmembrane proteins requires the Cornichon homologue Erv14p...
  34. pmc Role of the hydrophobic domain in targeting caveolin-1 to lipid droplets
    Anne G Ostermeyer
    Department of Biochemistry and Cell Biology, State University of New York at Stony Brook, Stony Brook, NY 11794 5215, USA
    J Cell Biol 164:69-78. 2004
    ..We propose that proper packing of putative hydrophobic helices may be required for LD targeting of caveolin-1...
  35. pmc A eukaryotic capsular polysaccharide is synthesized intracellularly and secreted via exocytosis
    Aki Yoneda
    Department of Molecular Microbiology, Washington University School of Medicine, St Louis, MO 63110, USA
    Mol Biol Cell 17:5131-40. 2006
    ..This strongly supports the conclusion that cryptococcal capsule is synthesized intracellularly and secreted via exocytosis...
  36. ncbi Purification, characterization and biological significance of tumor-derived exosomes
    Kenichiro Koga
    Department of Cancer Therapy and Research, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan
    Anticancer Res 25:3703-7. 2005
    ..With regard to their biological functions, Tu-exosomes cultured with a human breast cancer cell line bound to the cell surface and increased tumor cell proliferation. These data indicate that Tu-exosomes may have physiological functions...
  37. pmc SIVsm Tat, Rev, and Nef1: functional characteristics of r-GV internalization on isotypes, cytokines, and intracellular degradation
    Marinko Sremac
    Department of Microbiology, University of Massachusetts, Amherst, MA 01003, USA
    BMC Biotechnol 10:54. 2010
    ..1 macrophage cells to degrade these internalized display/delivery particles in vitro were examined...
  38. pmc Role of matrix vesicles in biomineralization
    Ellis E Golub
    Biochemistry Department, University of Pennsylvania School of Dental Medicine, 240 South 40th Street, Philadelphia, PA 19104, USA
    Biochim Biophys Acta 1790:1592-8. 2009
    ..During this period, their exact role, if any in the nucleation of hydroxyapatite mineral, and its subsequent association with the collagen fibrils in the organic matrix has been debated and remains controversial...
  39. pmc A key role for vesicles in fungal secondary metabolism
    Anindya Chanda
    Department of Food Science and Human Nutrition, National Food Safety and Toxicology Center, and Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI 48824
    Proc Natl Acad Sci U S A 106:19533-8. 2009
    ..We propose a unique 2-branch model to illustrate the proposed role for VeA in regulation of aflatoxisome development and aflatoxin gene expression...
  40. ncbi Investigating the role of murine epididymosomes and uterosomes in GPI-linked protein transfer to sperm using SPAM1 as a model
    Genevieve S Griffiths
    Department of Biological Sciences, University of Delaware, Newark, Delaware 19716, USA
    Mol Reprod Dev 75:1627-36. 2008
    ..Taken together, these results implicate vesicular docking as the mechanism of vesicle-mediated GPI-linked protein transfer to sperm from murine reproductive fluids...
  41. ncbi Prevention of microvesiculation by adhesion of buds to the mother cell membrane--a possible anticoagulant effect of healthy donor plasma
    Mojca Frank
    Department of Rheumatology, University Medical Centre Ljubljana, Ljubljana, Slovenia
    Autoimmun Rev 7:240-5. 2008
    ..Plasma that mediates stronger attractive interaction between GPVs might potentially be associated with a smaller risk of thrombosis...
  42. pmc Direct quantification of fusion rate reveals a distal role for AS160 in insulin-stimulated fusion of GLUT4 storage vesicles
    Li Jiang
    Joint Laboratory of Institute of Biophysics and Huazhong University of Science and Technology, National Laboratory of Biomacromolecules, Chinese Academy of Sciences, Beijing, China
    J Biol Chem 283:8508-16. 2008
    ....
  43. ncbi Proteomic analysis of dendritic cell-derived exosomes: a secreted subcellular compartment distinct from apoptotic vesicles
    C Thery
    Institut National de la Sante et de la Recherche Medical, Unité 520, Institut Curie, Paris, France
    J Immunol 166:7309-18. 2001
    ..Therefore, in addition to cytokines, dendritic cells produce a specific population of membrane vesicles, exosomes, with unique molecular composition and strong immunostimulating properties...
  44. ncbi Cell biology: The ins and outs of exosomes
    Jennifer Couzin
    Science 308:1862-3. 2005
  45. ncbi Cellular microparticles: new players in the field of vascular disease?
    M Diamant
    Department of Endocrinology Diabetes Centre, VU University Medical Centre, Amsterdam, The Netherlands
    Eur J Clin Invest 34:392-401. 2004
    ..In view of their functional properties, cell-derived microparticles may be an important intermediate in the cascade of cellular and plasmatic dysfunctions underlying the process of atherogenesis...
  46. pmc Acute hypertension provokes acute trafficking of distal tubule Na-Cl cotransporter (NCC) to subapical cytoplasmic vesicles
    Donna H Lee
    Dept of Cell and Neurobiology, Univ of Southern California Keck School of Medicine, 1333 San Pablo St, BMT 403, Los Angeles, CA 90089, USA
    Am J Physiol Renal Physiol 296:F810-8. 2009
    ..cotransporter (NCC) is regulated by trafficking between the apical plasma membrane (APM) and subapical cytoplasmic vesicles (SCV)...
  47. ncbi Centrosome-dependent anisotropic random walk of cytoplasmic vesicles
    Ivan V Maly
    Laboratory of Cell Motility, A N Belozersky Institute of Physico Chemical Biology, Moscow State University, Moscow, 119899, Russia
    Cell Biol Int 26:791-9. 2002
    We approach the problem of an apparently random movement of small cytoplasmic vesicles and its relationship to centrosome functioning...
  48. ncbi Role of EHD1 and EHBP1 in perinuclear sorting and insulin-regulated GLUT4 recycling in 3T3-L1 adipocytes
    Adilson Guilherme
    Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    J Biol Chem 279:40062-75. 2004
    ..Taken together, these results show that EHD1 and EHBP1, but not EHD2, are required for perinuclear localization of GLUT4 and reveal that loss of EHBP1 disrupts insulin-regulated GLUT4 recycling in cultured adipocytes...
  49. pmc Itinerant exosomes: emerging roles in cell and tissue polarity
    Aparna Lakkaraju
    Margaret M Dyson Vision Research Institute, Department of Ophthalmology, Weill Medical College of Cornell University, New York, NY 10021, USA
    Trends Cell Biol 18:199-209. 2008
    ..In this review, we discuss new evidence suggesting that exosomes participate in the transport of morphogens and RNA, and thus influence cell polarity and developmental patterning of tissues...
  50. ncbi The acrosome-acroplaxome-manchette complex and the shaping of the spermatid head
    Abraham L Kierszenbaum
    Department of Cell Biology and Anatomical Sciences, The Sophie Davis School of Biomedical Education The City University of New York Medical School, New York, NY 10031, USA
    Arch Histol Cytol 67:271-84. 2004
    ....
  51. ncbi Organization of interphase microtubules in fission yeast analyzed by electron tomography
    Johanna L Höög
    European Molecular Biology Laboratory, Cell Biology and Biophysics Program, D 69117 Heidelberg, Germany
    Dev Cell 12:349-61. 2007
    ..Our analysis shows that electron tomography of well-preserved cells is ideally suited for describing fine ultrastructural details that were not visible with previous techniques...
  52. pmc Heat shock protein 70 surface-positive tumor exosomes stimulate migratory and cytolytic activity of natural killer cells
    Robert Gastpar
    Department of Hematology and Oncology, University Hospital Regensburg, Regensburg, Germany
    Cancer Res 65:5238-47. 2005
    ..In summary, our data provide an explanation how Hsp70 reactivity in NK cells is induced by tumor-derived exosomes...
  53. pmc Nanoparticles isolated from blood: a reflection of vesiculability of blood cells during the isolation process
    Vid Sustar
    Laboratory of Clinical Biophysics, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia
    Int J Nanomedicine 6:2737-48. 2011
    ..We addressed this question by determination of the morphology and identity of nanoparticles harvested from blood...
  54. ncbi GLUT4 translocation: the last 200 nanometers
    Robert T Watson
    Department of Pharmacological Sciences, Stony Brook University, Stony Brook, NY 11794, USA
    Cell Signal 19:2209-17. 2007
    ..Future work will focus on identifying the key insulin targets that regulate the GLUT4 docking/fusion processes...
  55. pmc The conserved oligomeric Golgi complex is involved in double-membrane vesicle formation during autophagy
    Wei Lien Yen
    Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109, USA
    J Cell Biol 188:101-14. 2010
    ..In addition, mutations in the COG genes resulted in the mislocalization of Atg8 and Atg9, which are critical components involved in autophagosome formation...
  56. ncbi Vesicular transmitter release from astrocytes
    Vedrana Montana
    Department of Cell Biology and Neuroscience, Center for Glial Neuronal Interactions, University of California, Riverside, California, USA
    Glia 54:700-15. 2006
    ..The location of specific exocytotic sites on these cells, however, remains to be unequivocally determined...
  57. pmc GLUT4 retention in adipocytes requires two intracellular insulin-regulated transport steps
    Anja Zeigerer
    Department of Biochemistry, Biophysics and Molecular Medicine, Weill Medical College of Cornell University, New York, New York 10021, USA
    Mol Biol Cell 13:2421-35. 2002
    ..This compartment-based model provides the framework for understanding insulin-regulated trafficking at a molecular level...
  58. pmc Microvesicle-associated tissue factor and Trousseau's syndrome
    I del Conde
    Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, MA, USA
    J Thromb Haemost 5:70-4. 2007
    ..Trousseau's syndrome is a prothrombotic state associated with malignancy that is poorly understood pathophysiologically...
  59. pmc Insulin-regulated release from the endosomal recycling compartment is regulated by budding of specialized vesicles
    M A Lampson
    Program in Physiology, Biophysics, and Molecular Medicine, Weill Graduate School of Medical Sciences, Cornell University, New York, New York 10021, USA
    Mol Biol Cell 12:3489-501. 2001
    ....
  60. ncbi Cleavage of L1 in exosomes and apoptotic membrane vesicles released from ovarian carcinoma cells
    Paul Gutwein
    Tumor Immunology Programme, D010, German Cancer Research Center, Heidelberg, Germany
    Clin Cancer Res 11:2492-501. 2005
    ..Soluble L1 is present in serum and ascites of ovarian carcinoma patients. We investigated the mode of L1 cleavage and the function of soluble L1...
  61. ncbi Hemoglobin loss from erythrocytes in vivo results from spleen-facilitated vesiculation
    Frans L A Willekens
    Department of Clinical Chemistry, Rijnstate Hospital, Arnhem, The Netherlands
    Blood 101:747-51. 2003
    ..It is concluded that hemoglobin-containing vesicles within old RBCs are "pitted" by the spleen...
  62. pmc Accumulation of caveolin in the endoplasmic reticulum redirects the protein to lipid storage droplets
    A G Ostermeyer
    Department of Biochemistry and Cell Biology, State University of New York at Stony Brook, Stony Brook, New York 11794, USA
    J Cell Biol 152:1071-8. 2001
    ....
  63. pmc Alpha-synuclein-induced aggregation of cytoplasmic vesicles in Saccharomyces cerevisiae
    James H Soper
    Center for Neurodegenerative Disease Research and Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
    Mol Biol Cell 19:1093-103. 2008
    ....
  64. pmc A role for exosomes in the constitutive and stimulus-induced ectodomain cleavage of L1 and CD44
    Alexander Stoeck
    Tumor Immunology Programme, D010, German Cancer Research Center, Im Neuenheimer Feld 280, D 69120 Heidelberg, Germany
    Biochem J 393:609-18. 2006
    ..Our findings open up the possibility that exosomes serve as a platform for ectodomain shedding and as a vehicle for the cellular export of soluble molecules...
  65. ncbi The proteome of red cell membranes and vesicles during storage in blood bank conditions
    Giel J C G M Bosman
    Department of Biochemistry, the Center for Molecular and Biomolecular Informatics, Nijmegen Center of Molecular Life Sciences, Radboud University Nijmegen Medical Center, Nijmegen, The Netherlands
    Transfusion 48:827-35. 2008
    ..To be able to identify the mechanisms underlying these storage lesions, a proteomic analysis of the membranes of RBCs and their vesicles was performed during various periods of storage in blood bank conditions...
  66. ncbi Number of microvesicles in peripheral blood and ability of plasma to induce adhesion between phospholipid membranes in 19 patients with gastrointestinal diseases
    Rado Jansa
    Department of Gastroenterology, University Medical Centre Ljubljana, Ljubljana, Slovenia
    Blood Cells Mol Dis 41:124-32. 2008
    ..033) and smaller average effective angle of contact (difference 20%, statistical significance 0.013) compared to patients with other gastrointestinal diseases...
  67. ncbi Myosins II and V in chromaffin cells: myosin V is a chromaffin vesicle molecular motor involved in secretion
    Sergio D Rosé
    Secretory Process Research Program, Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, Ontario, Canada
    J Neurochem 85:287-98. 2003
    ..However, these results do not discard an indirect role for myosin II in secretion through its interaction with F-actin networks...
  68. pmc Tumor-derived microvesicles in sera of patients with head and neck cancer and their role in tumor progression
    Christoph Bergmann
    Departments of Pathology and Otolaryngology, University of Pittsburgh Cancer Institute, Pittsburgh, Pennsylvania, USA
    Head Neck 31:371-80. 2009
    ..We tested if MV molecular profile and activity correlate with disease progression...
  69. pmc Steady-state dynamics of Cajal body components in the Xenopus germinal vesicle
    Korie E Handwerger
    Department of Embryology, Carnegie Institution of Washington, Baltimore, MD 21210, USA
    J Cell Biol 160:495-504. 2003
    ..We propose that binding to other molecules and possibly assembly into larger complexes are the rate-limiting steps for FRAP of U7, coilin, and TBP inside CBs...
  70. pmc RASCAL is a new human cytomegalovirus-encoded protein that localizes to the nuclear lamina and in cytoplasmic vesicles at late times postinfection
    Matthew S Miller
    Department of Microbiology and Immunology, Schulich School of Medicine and Dentistry, The University of Western Ontario, London, Ontario N6A 5C1, Canada
    J Virol 84:6483-96. 2010
    ..Here, we describe the identification of a new CMV protein localizing to the nuclear envelope and in cytoplasmic vesicles at late times postinfection...
  71. pmc Interactions of unconjugated bilirubin with vesicles, cyclodextrins and micelles: new modeling and the role of high pKa values
    Pasupati Mukerjee
    School of Pharmacy, University of Wisconsin, Madison, 53705 2222, USA
    BMC Biochem 11:15. 2010
    ....
  72. pmc Dictyostelium discoideum expresses a malaria chloroquine resistance mechanism upon transfection with mutant, but not wild-type, Plasmodium falciparum transporter PfCRT
    Bronwen Naudé
    Laboratory of Malaria and Vector Research, NIAID, National Institutes of Health, Bethesda, Maryland 20892 8132, USA
    J Biol Chem 280:25596-603. 2005
    ..Transformed D. discoideum will be useful for further studies of the chloroquine resistance mechanism and may assist in the development and evaluation of new antimalarial drugs...
  73. ncbi Listeria monocytogenes L-forms respond to cell wall deficiency by modifying gene expression and the mode of division
    Simone Dell'Era
    Institute of Food Science and Nutrition, ETH Zurich, CH 8092 Zurich, Switzerland
    Mol Microbiol 73:306-22. 2009
    ....
  74. ncbi The actin-based motor myosin Va is a component of the acroplaxome, an acrosome-nuclear envelope junctional plate, and of manchette-associated vesicles
    A L Kierszenbaum
    Department of Cell Biology and Anatomical Sciences, The City University of New York Medical School The Sophie Davis School of Biomedical Education, New York, NY 10031, USA
    Cytogenet Genome Res 103:337-44. 2003
    ....
  75. ncbi The regulation of exosome secretion: a novel function of the p53 protein
    Xin Yu
    Cancer Institute of New Jersey, University of Medicine and Dentistry of New Jersey, New Brunswick, New Jersey, USA
    Cancer Res 66:4795-801. 2006
    ..Thus, the p53 pathway regulates the production of exosomes into the medium and these vesicles can communicate with adjacent cells and even cells of the immune system...
  76. ncbi Intravascular tissue factor initiates coagulation via circulating microvesicles and platelets
    Ingrid Muller
    Physiologisches Institut, Ludwig Maximilians Universitat Munchen, 81377 Munchen, Germany
    FASEB J 17:476-8. 2003
    ....
  77. pmc A caveolin dominant negative mutant associates with lipid bodies and induces intracellular cholesterol imbalance
    A Pol
    Institute for Molecular Bioscience, Centre for Microscopy and Microanalysis and Department of Physiology and Pharmacology, University of Queensland, Queensland 4072, Australia
    J Cell Biol 152:1057-70. 2001
    ..We conclude that caveolin can associate with the membrane surrounding lipid droplets and is a key component involved in intracellular cholesterol balance and lipid transport in fibroblasts...
  78. ncbi The PtdIns3P phosphatase myotubularin is a cytoplasmic protein that also localizes to Rac1-inducible plasma membrane ruffles
    Jocelyn Laporte
    Institut de Genetique et de Biologie Moleculaire et Cellulaire, CNRS INSERM ULP, 1 rue Laurent Fries, BP 10142, 67404 Illkirch Cedex, CU de Strasbourg, France
    J Cell Sci 115:3105-17. 2002
    ..We thus propose that myotubularin may dephosphorylate a subpool of PtdIns3P (or another related substrate) at the plasma membrane...
  79. ncbi Isolation of calcifiable vesicles from aortas of rabbits fed with high cholesterol diets
    H H Hsu
    Department of Pathology and Laboratory Medicine, University of Kansas Medical Center, Kansas City 66160 7410, USA
    Atherosclerosis 153:337-48. 2000
    ..Altogether, these observations suggest that accumulation of the released calcifiable vesicles, as a result of high cholesterol diets, may have a role in dystrophic calcification in hyperlipidemia-related atherosclerosis...
  80. pmc A role for BARS at the fission step of COPI vesicle formation from Golgi membrane
    Jia Shu Yang
    Division of Rheumatology, Immunology and Allergy, Brigham and Women s Hospital, Boston, MA 02115, USA
    EMBO J 24:4133-43. 2005
    ..Our findings not only identify a new factor needed for COPI vesicle formation from Golgi membrane but also reveal a surprising mechanism by which the roles of p-coA and GAP are linked in this process...
  81. pmc Evidence that Golgi structure depends on a p115 activity that is independent of the vesicle tether components giantin and GM130
    M A Puthenveedu
    Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA 15213, USA
    J Cell Biol 155:227-38. 2001
    ..These observations indicate that inhibition of p115 can induce a mitotic-like Golgi disassembly, but its essential role in Golgi structure is independent of its Golgi-localized binding partners giantin and GM130...
  82. pmc Cog3p depletion blocks vesicle-mediated Golgi retrograde trafficking in HeLa cells
    Sergey N Zolov
    Department of Physiology and Biophysics, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA
    J Cell Biol 168:747-59. 2005
    ..In a contrast, Cog3p KD resulted in inhibition of retrograde trafficking of the Shiga toxin. Furthermore, the mammalian COG complex physically interacts with GS28 and COPI and specifically binds to isolated CCD vesicles...
  83. pmc Induction of maturation-promoting factor during Xenopus oocyte maturation uncouples Ca(2+) store depletion from store-operated Ca(2+) entry
    Khaled Machaca
    Department of Physiology and Biophysics, University of Arkansas Medical Science, Little Rock, 72205, USA
    J Cell Biol 156:75-85. 2002
    ..We further show that preactivated SOCE is not inactivated by MPF, suggesting that MPF does not block Ca(2+) influx through SOCE channels, but rather inhibits coupling between store depletion and SOCE activation...
  84. ncbi Phenotypic and functional characterization of intestinal epithelial exosomes
    J Mallegol
    INSERM EMI 0212, Faculté Necker Enfants Malades, 156 rue de Vaugirard, 75730 Paris, France
    Blood Cells Mol Dis 35:11-6. 2005
    ....
  85. ncbi Cassette-based presentation of SIV epitopes with recombinant gas vesicles from halophilic archaea
    Elizabeth S Stuart
    Department of Microbiology, University of Massachusetts, Amherst, MA 01003, USA
    J Biotechnol 114:225-37. 2004
    ....
  86. pmc ADP ribosylation factor 6 is activated and controls membrane delivery during phagocytosis in macrophages
    Florence Niedergang
    Membrane and Cytoskeleton Dynamics Laboratory, UMR144 CNRS, Institut Curie, 26 Rue d Ulm, F 75248 Paris Cedex 05, France
    J Cell Biol 161:1143-50. 2003
    ..This resulted in an early blockade in pseudopod extension and accumulation of intracellular vesicles, as observed by electron microscopy. We conclude that ARF6 is a major regulator of membrane recycling during phagocytosis...
  87. pmc Recombinant gas vesicles from Halobacterium sp. displaying SIV peptides demonstrate biotechnology potential as a pathogen peptide delivery vehicle
    Marinko Sremac
    Department of Microbiology, University of Massachusetts, Amherst, MA 01003, USA
    BMC Biotechnol 8:9. 2008
    ..Tests using mice indicated that the humoral immune response to the gag encoded sequences evoked immunologic memory in the absence of an exogenous adjuvant...
  88. pmc Platelet-derived exosomes induce endothelial cell apoptosis through peroxynitrite generation: experimental evidence for a novel mechanism of septic vascular dysfunction
    Marcela Helena Gambim
    Division of Rheumatology, University of Sao Paulo School of Medicine, Avenida Doutor Arnaldo, 455, 01246 903, Sao Paulo, SP
    Crit Care 11:R107. 2007
    ..We sought to further characterize the microparticle-dependent vascular injury pathway...
  89. ncbi Depletion of beta-COP reveals a role for COP-I in compartmentalization of secretory compartments and in biosynthetic transport of caveolin-1
    Melanie L Styers
    Department of Cell Biology, University of Alabama at Birmingham, Birmingham, AL 35294, USA
    Am J Physiol Cell Physiol 294:C1485-98. 2008
    ..Our findings suggest that COP-I is required for the compartmentalization of the ERGIC, Golgi, TGN, and recycling endosomes and that COP-I plays a novel role in the biosynthetic transport of Cav1...
  90. ncbi Hermansky-Pudlak syndrome: vesicle formation from yeast to man
    Marjan Huizing
    National Human Genome Research Institute, National Institutes of Health, Bethesda, MD 20892 1851, USA
    Pigment Cell Res 15:405-19. 2002
    ..Pursuit of the mechanism of mammalian vesicle formation and trafficking, impaired in HPS, relies upon investigation of these mouse models as well as studies of protein complexes involved in yeast vacuole formation...
  91. ncbi Mechanism of dystrophic calcification in rabbit aortas: temporal and spatial distributions of calcifying vesicles and calcification-related structural proteins
    Howard H T Hsu
    Department of Pathology and Laboratory Medicine, University of Kansas Medical Center, Kansas City, KS 66160 7410, USA
    Cardiovasc Pathol 13:3-10. 2004
    ..Whether the distributions of other structural proteins related to calcification such as collagen and elastin fibers undergo coordinated modifications has not been established...
  92. pmc Tyrosine-phosphorylated extracellular signal--regulated kinase associates with the Golgi complex during G2/M phase of the cell cycle: evidence for regulation of Golgi structure
    H Cha
    Department of Pharmaceutical Sciences, University of Maryland, School of Pharmacy, Baltimore, Maryland 21201, USA
    J Cell Biol 153:1355-67. 2001
    ..These data provide the first evidence that pY ERK and not ERK kinase activity regulates Golgi structure and may be involved in mitotic Golgi fragmentation and reformation...
  93. pmc Vesicle trafficking and cell surface membrane patchiness
    Q Tang
    Department of Biology, The Johns Hopkins University, Baltimore, Maryland 21218, USA
    Biophys J 81:196-203. 2001
    ..As predicted by the simulation, the apparent sizes of HLA patches increased, whereas their intensities decreased after endocytosis and vesicle trafficking were inhibited...
  94. ncbi The mass action hypothesis: formation of Glut4 storage vesicles, a tissue-specific, regulated exocytic compartment
    P F Pilch
    Department of Biochemistry, Boston University School of Medicine, Boston, MA 02118, USA
    Acta Physiol (Oxf) 192:89-101. 2008
    ..I review herein the ontogeny of GSVs and their composition as it relates to a tissue-specific, hormone-sensitive exocytic compartment and propose a mechanism for their formation...
  95. ncbi Unsolved mysteries in membrane traffic
    Suzanne R Pfeffer
    Department of Biochemistry, Stanford University School of Medicine, Stanford, California 94305 5307, USA
    Annu Rev Biochem 76:629-45. 2007
    ..But much remains to be learned. This review highlights underlying themes in membrane traffic to help us refocus and solve many remaining and newly emerging issues that are fundamental to mammalian cell biology and human physiology...
  96. ncbi Sortilin is essential and sufficient for the formation of Glut4 storage vesicles in 3T3-L1 adipocytes
    Jun Shi
    Boston University School of Medicine, Boston, Massachusetts 02118, USA
    Dev Cell 9:99-108. 2005
    ..Thus, sortilin is not only essential, but also sufficient for biogenesis of GSVs and acquisition of insulin responsiveness in adipose cells...
  97. ncbi Frizzled-7 turnover at the plasma membrane is regulated by cell density and the Ca(2+) -dependent protease calpain-1
    Ian T Struewing
    Graduate Center for Nutritional Sciences, University of Kentucky, Lexington, Lexington, KY 40536, USA
    Exp Cell Res 313:3526-41. 2007
    ..Altogether, our findings pinpoint calpain-1 as a regulator of Frizzled-7 turnover at the plasma membrane and reveal a link between Frizzled-7 cleavage and its activity...
  98. ncbi Protein kinase D 3 is localized in vesicular structures and interacts with vesicle-associated membrane protein 2
    Ganwei Lu
    Department of Pharmacology, University of Pittsburgh, Pittsburgh, PA 15261, USA
    Cell Signal 19:867-79. 2007
    ....
  99. pmc v-SNAREs control exocytosis of vesicles from priming to fusion
    Maria Borisovska
    Department of Physiology, University of Saarland, Homburg Saar, Germany
    EMBO J 24:2114-26. 2005
    ..We demonstrate that dynamics of fusion pore dilation are regulated by v-SNAREs, indicating their action throughout exocytosis from priming to fusion of vesicles...
  100. pmc Regulation of caveolin-1 membrane trafficking by the Na/K-ATPase
    Ting Cai
    Department of Physiology and Pharmacology, University of Toledo College of Medicine, Health Science Campus, Toledo, OH 43614, USA
    J Cell Biol 182:1153-69. 2008
    ..Finally, Na/K-ATPase knockdown has no effect on processing or exit of Cav1 from the Golgi. Thus, the Na/K-ATPase regulates Cav1 endocytic trafficking and stabilizes the Cav1 plasma membrane pool...
  101. ncbi Characterization of the human GARP (Golgi associated retrograde protein) complex
    Heike Liewen
    Medical Department I, University of the Saarland, Homburg 66421, Germany
    Exp Cell Res 306:24-34. 2005
    ..Taken together, GARP shows significant conservation between various species but diversification and specialization result in important differences in human cells...

Research Grants62

  1. REGULATION AND FUNCTION OF STE20-RELATED PROTEIN KINASES
    Melanie H Cobb; Fiscal Year: 2012
    ..Finally, we will explore the actions of WNK1 on vesicular trafficking. WNK1 is localized on numerous small cytoplasmic vesicles and increases the amount of transforming growth factor (TGF) 2 receptors and other proteins on the plasma ..
  2. Sodium-chloride co-transporter regulation in the kidney
    Alicia A McDonough; Fiscal Year: 2013
    ..We provided in vivo evidence that NCC redistributes out of the APM into subapical cytoplasmic vesicles (SCV) during high NaCl diet and ACE inhibition and redistributes into the APM from SCV during low NaCl diet ..
  3. Characterization of AQP2 interacting proteins and novel functions of AQP2
    HUA A LU; Fiscal Year: 2010
    ..It functions by recruiting the AQP2 water channel from cytoplasmic vesicles to the plasma membrane of collecting duct principal cells...
  4. RASCAL: a new tropism determinant encoded by human cytomegalovirus?
    Laura Hertel; Fiscal Year: 2012
    ..localization of RASCALTB40/E and RASCALTowne, and both isoforms remained associated with lamin B-positive cytoplasmic vesicles during virion egress from the nucleus...
  5. EBV EXPRESSION IN NASOPHARYNGEAL CARCINOMA
    NANCY JOAN RAAB-TRAUB; Fiscal Year: 2013
    ..effects of LMP1 on signaling are mediated by unique complexes that form within plasma membrane domains and cytoplasmic vesicles. We suggest that LMP1 modulates the constituents of lipid raft domains to form exosomes that affect the ..
  6. Acinar Biology and Pancreatic Disease
    Guy E Groblewski; Fiscal Year: 2011
    ..directly regulates lysosmal membrane exocytosis and when over-expressed induces a massive accumulation of cytoplasmic vesicles. Biochemical data indicate that D52 is a phosphatidylinositol-3-phosphate binding protein that copurifies ..
  7. Endothelial SK3 Channel Modulation of EDHF is Estrogen Regulated
    Mike T Lin; Fiscal Year: 2013
    ..Endothelial SKS channels are localized within caveolae dynamically trafficked surface invaginations and cytoplasmic vesicles that juxtapose receptors, ion channels, transporters, and signaling molecules that mediate the transport of ..
  8. Acinar Biology and Pancreatic Disease
    Guy E Groblewski; Fiscal Year: 2013
    ..directly regulates lysosmal membrane exocytosis and when over-expressed induces a massive accumulation of cytoplasmic vesicles. This proposal aims to delineate the precise functional roles of VAMP2- and VAMP8- positive ZGs in the ..
  9. CHOLESTEROL AND NEURODEGENERATION
    Shutish Patel; Fiscal Year: 2001
    ..microscopy and a panel of anti-peptide antibodies to functional domains of NPCI, we have found NPC1 in cytoplasmic vesicles that are delineated by the lysosome associated membrane glycoprotein 2 (LAMP2)...
  10. BASOPHIL/MAST CELL FUNCTION
    Ann Dvorak; Fiscal Year: 1991
    ..functions of basophils and mast cells are mediated by a bidirectional flow of small, membrane bound cytoplasmic vesicles between the plasma membrane and the cytoplasmic granules...
  11. BASOPHIL AND MAST CELL VESICULAR TRANSPORT MECHANISMS
    GARY LOGIN; Fiscal Year: 1991
    ..functions of basophils and mast cells may be mediated by a bidirectional flow of small, membrane bound cytoplasmic vesicles between the plasma memberane and the cytoplasmic granules...
  12. FUNCTIONS OF CYTOPLASMIC DYNEIN
    J McIntosh; Fiscal Year: 1999
    ..During interphase, it is associated with cytoplasmic vesicles and perhaps with primary endosomes. DHC2 is concentrated in the Golgi apparatus and on cytoplasmic vesicles...
  13. EXTRACELLULAR MODULATION OF UROTHELIAL PERMEABILITY
    Simon Lewis; Fiscal Year: 2000
    ..a minimum ratio for apical membrane surface area to urine volume, by the withdrawal and insertion of cytoplasmic vesicles at the apical membrane...
  14. Regulation of Mammalian Copper Homeotasis
    MICHAEL PETRIS; Fiscal Year: 2007
    ..ATP7A and ATP7B, which are normally located in the trans-Golgi network, are stimulated to relocate to cytoplasmic vesicles or the plasma membrane by elevated copper to facilitate copper efflux from the cytoplasm...
  15. DYNAMIN LIKE PROTEIN IN HEPATOCELLULAR TRANSPORT
    Yisang Yoon; Fiscal Year: 2001
    ..that this dynamin-like protein, DLP1, is expressed in hepatocytes where it associates with numerous motile cytoplasmic vesicles, tubules of the endoplasmic reticulum (ER), and mitochondria...
  16. SERINE PROTEINASES IN APICOMPLEXAN PARASITES
    Kami Kim; Fiscal Year: 2009
    ..depletion of TgSUB2 results in parasites that divide poorly, have few rhoptries, and have large aberrant cytoplasmic vesicles. In contrast, TgSUB1 disruptants are viable...
  17. CELLULAR REGULATION OF PHOSPATE TRANSPORT IN KIDNEY
    Thomas Dousa; Fiscal Year: 1993
    ..modulate the balance between active Na-Pi-COT inserted into BBM and nonfunctional Na-Pi-COT located in cytoplasmic vesicles (membrane recycling)...
  18. VESICLE TRAFFICKING AND CL-TRANSPORT IN CF CELLS
    Kevin Kirk; Fiscal Year: 1993
    ..membrane Cl- permeability in epithelial cells is acutely regulated by a recycling of Cl- channels between cytoplasmic vesicles and plasma membrane and iii) to identify and, characterize additional Cl- channels within cytoplasmic ..
  19. Intestinal ferroportin 1 expression and iron absorption
    Kwo Yih Yeh; Fiscal Year: 2009
    ..The results should reveal mechanisms involved in the regulation and function of FPT1 and extend our understanding of intestinal iron absorption and body iron homeostatic regulation. ..
  20. MICROTUBULE/KERATIN INTERACTIONS DURING SPERMATOGENESIS
    ABRAHAM KIERSZENBAUM; Fiscal Year: 2002
    ..Engineering mouse models in which the expression of K9 is null, is important for both reproductive biology and cancer research. ..
  21. Mineralization studies related to atherosclerosis
    HOWARD HSU; Fiscal Year: 2004
    ..abstract_text> ..
  22. CONTROL OF THE EXIT FROM MITOSIS IN FISSION YEAST
    Shelley Sazer; Fiscal Year: 2002
    ..Test the hypothesis that the nuclear envelope is a downstream target of the spi1 GTPase system. ..
  23. Hematopoietic Circadian Rhythms
    Peter Quesenberry; Fiscal Year: 2005
    ..Transgenic Pert-luc rats and mice will be available at University of Virginia, and the array/informatic studies done at Princeton. ..
  24. ACOUSTICALLY ACTIVATED MICELLAR DRUG DELIVERY
    Natalya Rapoport; Fiscal Year: 2007
    ..abstract_text> ..
  25. Mechanisms of Ataxin-1 Mediated Purkinje Cell Death
    PARMINDER VIG; Fiscal Year: 2007
    ..Further, changes observed in SCA-1 patients and in transgenic mice will be compared with that in Machado-Joseph disease/SCA-3, other cerebellar ataxias and normal controls. ..
  26. Iron and copper transporter trafficking in healthy and diseased melanocytes
    Michael Marks; Fiscal Year: 2007
    ..This proposal tests the hypothesis that Hermansky Pudlak Syndrome results from iron and copper dysregulation in the affected cell types. [unreadable] [unreadable] [unreadable]..
  27. INTRACELLULAR TRANSPORT--THE MANNOSE-PHOSPHATE RECEPTOR
    Suzanne Pfeffer; Fiscal Year: 2008
    ..These studies have important implications for our understanding of growth control and antigen processing, and will provide fundamental information regarding the mechanism of receptor trafficking in mammalian cells. ..
  28. A novel Dlg isoform and identification of its interacting proteins
    Kyung Ok Cho; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  29. Mechanism and function of a novel actin nucleator
    R Mullins; Fiscal Year: 2009
    ..2. Characterize the interaction of Cappuccino with actin. 3. Characterize the interaction between Spir and Cappuccino. 4. Characterize the interaction of the Spir FYVE domain with lipids. ..
  30. Omega-6/Omega-3 Fatty Acid Ratio and Tumorigenesis
    Jing Kang; Fiscal Year: 2009
    ..Information derived from these studies will increase our understanding of the importance of n-3 fatty acids as well as n-6/n-3 fatty acid ratio in cancer prevention and help guide dietary advice. ..
  31. HERPES SIMPLEX VIRUS TERMINASES
    Joel Baines; Fiscal Year: 2008
    ..The studies in this aim may be important not only for understanding HSV DNA cleavage and packaging, but will likely provide insight into under-explored functions of the nucleus. ..
  32. Models of P. carinii Infection: SP-A and SP-D null mice
    Michael Beers; Fiscal Year: 2009
    ....
  33. Glutathione Peroxidase & Redox State in Atherosclerosis
    Francis Miller; Fiscal Year: 2009
    ..Information gained from these studies will provide a foundation for additional studies of redox status in vascular disease and potential novel therapeutic strategies to modify the progression of atherosclerosis in patients. ..
  34. Molecular Analysis of the CCC185 Golgin
    Suzanne R Pfeffer; Fiscal Year: 2010
    ..This work has broad implications for our understanding of vesicle docking and fusion events within the secretory and endocytic pathways that are essential for normal human health and disease. ..
  35. Maturation functions of the HSV-1 tegument
    Prashant Desai; Fiscal Year: 2010
    ..translocation, Golgi stabilization, Golgi trafficking, virion incorporation and protein-protein interactions This analysis will identify domains of the proteins that have the potential to be used as targets for antiviral intervention ..
  36. INSULIN REGULATION OF CELL NUTRITION
    Konstantin Kandror; Fiscal Year: 2008
    ..In Specific Aim 3, we will characterize the significance of Glut4-mediated glucose uptake in the cerebellum under various experimental conditions in vivo. ..
  37. MOLECULAR TOXICOLOGY IN HUMAN KIDNEY CELLS
    Lawrence Lash; Fiscal Year: 2007
    ..Achievement of these aims should build on our previous work in human kidney cells and extend it to provide a much more complete understanding of the various and complex ways in which DCVC affects the human kidney [unreadable] [unreadable]..
  38. RETINOIDS, M6P/IGF II RECEPTOR & CELL GROWTH REGULATION
    Jing Kang; Fiscal Year: 2002
    ..This knowledge may lead to novel or more effective therapeutic and preventive approaches for cancer and developmental defects. ..
  39. Chlamydia trachomatis, Genital Flora, & STD Pathogenesis
    Gerald Byrne; Fiscal Year: 2004
    ..This hypothesis will be tested by determining the effects of indole-producing normal flora on chlamydial genital tract disease in a well-characterized murine model. ..
  40. T CELL TURNOVER AND THE ROLE OF CYTOKINES
    Jonathan Sprent; Fiscal Year: 2004
    ....
  41. Novel Approaches to the Assessment of Oral Mucosal Immu*
    Theresa Whiteside; Fiscal Year: 2003
    ..A better understanding of oral immunity in normal and HIV-infected mucosa is important for the development of new antiviral therapies, including oral antiviral vaccines. ..
  42. CELLULAR PHYSIOLOGY OF STAT3
    PRAVIN SEHGAL; Fiscal Year: 2002
    ..Establishing the basic subunit structure of the cytosolic 200-400 kDa and 1-2 MDa STAT3-containing complexes in IL-6-free and IL-6-treated cells is a sine qua non for further progress in this field. ..
  43. Challenges in the Era of Stem Cell Plasticity
    Peter Quesenberry; Fiscal Year: 2003
    ..Participants will include investigators in the basic biology of stem cells, clinical transplanters, and clinician investigators. The workshops and symposium will be held in Providence, Rhode Island on April 8, 9, 10 & 11, 2003 ..
  44. IN VITRO EVOLUTION OF NEW MOLECULAR FUNCTIONS
    Jack Szostak; Fiscal Year: 2003
    ..The re-use of previously selected functions to evolve new functions will also be studied. The idea that proteins are better than RNA at evolving new functions from existing functions will also be tested. ..
  45. LENS MEMBRANE STEROID BINDING PROTEIN (MSRP)
    RICHARD CENEDELLA; Fiscal Year: 2002
    ..Preliminary findings and published data support the possibility that steroids can rapidly elevate intracellular calcium and inhibit Na+/K+-ATPase activity in lens epithelial cells. ..
  46. Conference on Photodynamic Therapy
    David Kessel; Fiscal Year: 2002
    ..The most recent PDT meeting, sponsored by the International Photodynamic Assn, has more than 600 participants. ..
  47. B cell functional and signaling abnormalities in SLE
    Jennifer Anolik; Fiscal Year: 2006
    ..abstract_text> ..
  48. Function of a-1-Antitrypsin Receptor in Lung Disease
    William Balch; Fiscal Year: 2006
    ..unreadable] [unreadable]..
  49. Beta2 Adrenergic Receptor in Keratinocyte Migration
    CHRISTINE PULLAR; Fiscal Year: 2006
    ..abstract_text> ..
  50. DNA-Based Vaccines for Oral Carcnoma
    Theresa Whiteside; Fiscal Year: 2005
    ....
  51. Lens Cholesterol Metobolism and the U1866A Cataract.
    RICHARD CENEDELLA; Fiscal Year: 2005
    ..These are obvious benefits to public health considering the intended life-long use of the drugs. ..
  52. Development of ERK docking domain inhibitors
    Paul Shapiro; Fiscal Year: 2005
    ..These studies represent a novel approach for developing compounds that selectively block ERK activation of proteins involved in cancer cell proliferation. ..
  53. GLUTATHIONE AND MITOCHONDRIA IN TOXIC RENAL INJURY
    Lawrence Lash; Fiscal Year: 2003
    ..This information may have therapeutic applications for prevention of renal cellular injury or for understanding mitochondrial diseases or age-related decreases that occur in mitochondrial function. ..
  54. Substrate-Selective Inhibitors of Secretory and Membrane Protein Biogenesis
    Jack Taunton; Fiscal Year: 2010
    ..The major goal of this project is to design and synthesize new chemical tools that will allow us to dissect the molecular basis of cotransin's biological activity. cotransin ..
  55. Role of Cholesterol Homeostasis in Macular Degeneration
    Aparna Lakkaraju; Fiscal Year: 2006
    ....
  56. SURFACTANT METABOLISM IN PNEUMOCYSTIS CARINII PNEUMONIA
    Michael Beers; Fiscal Year: 2002
    ..A co-investigator experienced with P. carinii biology and gpA purification; 3. Consultants recognized as authorities on the scid mouse model of PCP and on isolated lung perfusion. ..
  57. PICORNAVIRAL 3A PROTEINS AND HOST PROTEIN SECRETION
    Karla Kirkegaard; Fiscal Year: 2005
    ..It is anticipated that this method will allow the regulation of the antigen presentation to the cellular immune response by picornaviral-based vectors, which could be crucial in vaccine design. ..
  58. Tumor-reactive IgG in the diagnosis of ovarian cancer
    Douglas Taylor; Fiscal Year: 2005
    ..Ultimately, antigenic proteins, linked with histologic type and stage of disease, will be combined into the development of protein arrays to screen for circulating reactive IgG as an early diagnostic test. ..
  59. Junction Adhesion Molecule CAR and the Immune System
    Steven Carson; Fiscal Year: 2007
    ..This work will advance knowledge of cells that form tight _junctions and their interactions with cells and molecules that must cross those barriers. ..