mitotic spindle apparatus

Summary

Summary: An organelle consisting of three components: (1) the astral microtubules, which form around each centrosome and extend to the periphery; (2) the polar microtubules which extend from one spindle pole to the equator; and (3) the kinetochore microtubules, which connect the centromeres of the various chromosomes to either centrosome.

Top Publications

  1. pmc The protein composition of mitotic chromosomes determined using multiclassifier combinatorial proteomics
    Shinya Ohta
    Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh EH9 3JR, UK
    Cell 142:810-21. 2010
  2. pmc A mechanism linking extra centrosomes to chromosomal instability
    Neil J Ganem
    Howard Hughes Medical Institute, Department of Pediatric Oncology, Dana Farber Cancer Institute, Children s Hospital, Boston, Massachusetts 02115, USA
    Nature 460:278-82. 2009
  3. pmc Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes
    Beate Neumann
    MitoCheck Project Group, European Molecular Biology Laboratory EMBL, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    Nature 464:721-7. 2010
  4. ncbi Mechanisms of asymmetric stem cell division
    Juergen A Knoblich
    Institute of Molecular Biotechnology of the Austrian Academy of Sciences, Dr Bohr Gasse 3, 1030 Vienna, Austria
    Cell 132:583-97. 2008
  5. ncbi The spindle-assembly checkpoint in space and time
    Andrea Musacchio
    Department of Experimental Oncology, European Institute of Oncology, Via Ripamonti 435, 20141 Milan, Italy
    Nat Rev Mol Cell Biol 8:379-93. 2007
  6. pmc Asymmetric cell divisions promote stratification and differentiation of mammalian skin
    Terry Lechler
    Howard Hughes Medical Institute, The Rockefeller University, New York, New York 10021, USA
    Nature 437:275-80. 2005
  7. pmc Systematic analysis of human protein complexes identifies chromosome segregation proteins
    James R A Hutchins
    Research Institute of Molecular Pathology IMP, Dr Bohr Gasse 7, A 1030 Vienna, Austria
    Science 328:593-9. 2010
  8. pmc Dissecting the role of MPS1 in chromosome biorientation and the spindle checkpoint through the small molecule inhibitor reversine
    Stefano Santaguida
    Department of Experimental Oncology, European Institute of Oncology, I 20139 Milan, Italy
    J Cell Biol 190:73-87. 2010
  9. doi Mitotic spindle orientation in asymmetric and symmetric cell divisions during animal development
    Xavier Morin
    Institut de Biologie de l Ecole Normale Supérieure, 46 rue d Ulm, Paris, France
    Dev Cell 21:102-19. 2011
  10. pmc Survivin reads phosphorylated histone H3 threonine 3 to activate the mitotic kinase Aurora B
    Alexander E Kelly
    Laboratory of Chromosome and Cell Biology, The Rockefeller University, New York, NY 10065, USA
    Science 330:235-9. 2010

Detail Information

Publications245 found, 100 shown here

  1. pmc The protein composition of mitotic chromosomes determined using multiclassifier combinatorial proteomics
    Shinya Ohta
    Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh EH9 3JR, UK
    Cell 142:810-21. 2010
    ..Our integrated analysis predicts that up to 97 new centromere-associated proteins remain to be discovered in our data set...
  2. pmc A mechanism linking extra centrosomes to chromosomal instability
    Neil J Ganem
    Howard Hughes Medical Institute, Department of Pediatric Oncology, Dana Farber Cancer Institute, Children s Hospital, Boston, Massachusetts 02115, USA
    Nature 460:278-82. 2009
    ..These findings provide a direct mechanistic link between extra centrosomes and CIN, two common characteristics of solid tumours. We propose that this mechanism may be a common underlying cause of CIN in human cancer...
  3. pmc Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes
    Beate Neumann
    MitoCheck Project Group, European Molecular Biology Laboratory EMBL, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    Nature 464:721-7. 2010
    ..As part of the Mitocheck consortium, this study provides an in-depth analysis of cell division phenotypes and makes the entire high-content data set available as a resource to the community...
  4. ncbi Mechanisms of asymmetric stem cell division
    Juergen A Knoblich
    Institute of Molecular Biotechnology of the Austrian Academy of Sciences, Dr Bohr Gasse 3, 1030 Vienna, Austria
    Cell 132:583-97. 2008
    ..These distinct pathways have been elucidated mostly in Drosophila. Although the molecules involved are highly conserved in vertebrates, the way they act is tissue specific and sometimes very different from invertebrates...
  5. ncbi The spindle-assembly checkpoint in space and time
    Andrea Musacchio
    Department of Experimental Oncology, European Institute of Oncology, Via Ripamonti 435, 20141 Milan, Italy
    Nat Rev Mol Cell Biol 8:379-93. 2007
    ..These studies are finally starting to reveal the mechanisms of checkpoint activation and silencing during mitotic progression...
  6. pmc Asymmetric cell divisions promote stratification and differentiation of mammalian skin
    Terry Lechler
    Howard Hughes Medical Institute, The Rockefeller University, New York, New York 10021, USA
    Nature 437:275-80. 2005
    ..We further demonstrate that integrins and cadherins are essential for the apical localization of atypical protein kinase C, the Par3-LGN-Inscuteable complex and NuMA-dynactin to align the spindle...
  7. pmc Systematic analysis of human protein complexes identifies chromosome segregation proteins
    James R A Hutchins
    Research Institute of Molecular Pathology IMP, Dr Bohr Gasse 7, A 1030 Vienna, Austria
    Science 328:593-9. 2010
    ..The approaches we describe here are generally applicable to high-throughput follow-up analyses of phenotypic screens in mammalian cells...
  8. pmc Dissecting the role of MPS1 in chromosome biorientation and the spindle checkpoint through the small molecule inhibitor reversine
    Stefano Santaguida
    Department of Experimental Oncology, European Institute of Oncology, I 20139 Milan, Italy
    J Cell Biol 190:73-87. 2010
    ..They also shed light on the relationship between the error correction pathway and the spindle checkpoint and suggest that these processes are coregulated and are likely to share at least a subset of their catalytic machinery...
  9. doi Mitotic spindle orientation in asymmetric and symmetric cell divisions during animal development
    Xavier Morin
    Institut de Biologie de l Ecole Normale Supérieure, 46 rue d Ulm, Paris, France
    Dev Cell 21:102-19. 2011
    ..Many recent studies show that future advances in the field of mitotic spindle orientation will arise from combinations of physical perturbation and modeling with classical genetics and developmental biology approaches...
  10. pmc Survivin reads phosphorylated histone H3 threonine 3 to activate the mitotic kinase Aurora B
    Alexander E Kelly
    Laboratory of Chromosome and Cell Biology, The Rockefeller University, New York, NY 10065, USA
    Science 330:235-9. 2010
    ..These findings establish a direct cellular role for mitotic histone H3T3 phosphorylation, which is read and translated by the CPC to ensure accurate cell division...
  11. pmc Asymmetric cell divisions promote Notch-dependent epidermal differentiation
    Scott E Williams
    Howard Hughes Medical Institute, Laboratory of Mammalian Cell Biology and Development, The Rockefeller University, New York, New York 10065, USA
    Nature 470:353-8. 2011
    ....
  12. pmc Histone H3 Thr-3 phosphorylation by Haspin positions Aurora B at centromeres in mitosis
    Fangwei Wang
    Division of Rheumatology, Immunology, and Allergy, Brigham and Women s Hospital, Harvard Medical School, Smith Building, 1 Jimmy Fund Way, Boston, MA 02115, USA
    Science 330:231-5. 2010
    ..Therefore, H3T3ph generated by Haspin positions the CPC at centromeres to regulate selected targets of Aurora B during mitosis...
  13. doi Huntingtin is required for mitotic spindle orientation and mammalian neurogenesis
    Juliette D Godin
    Institut Curie, Orsay F 91405, France
    Neuron 67:392-406. 2010
    ..Moreover, Drosophila huntingtin restores spindle misorientation in mammalian cells. These findings reveal an unexpected role for huntingtin in dividing cells, with potential important implications in health and disease...
  14. ncbi Microtubule-organizing centres: a re-evaluation
    Jens Lüders
    Department of Biological Sciences, Stanford University, CA 94305, USA
    Nat Rev Mol Cell Biol 8:161-7. 2007
    ..These findings suggest that the spatio-temporal control of microtubule nucleation is more complex than previously thought, leading us to a re-evaluation of the concept of the microtubule-organizing center...
  15. pmc Chromosome- and spindle-pole-derived signals generate an intrinsic code for spindle position and orientation
    Tomomi Kiyomitsu
    Whitehead Institute, Nine Cambridge Center, Cambridge, Massachusetts 02142, USA
    Nat Cell Biol 14:311-7. 2012
    ..We propose that these chromosome- and spindle-pole-derived gradients generate an intrinsic code to control spindle position and orientation...
  16. doi External forces control mitotic spindle positioning
    Jenny Fink
    Institut Curie, CNRS UMR 144, 26 Rue d Ulm, 75248 Paris Cedex 05, France
    Nat Cell Biol 13:771-8. 2011
    ..We propose that cells divide according to cues provided by their mechanical micro-environment, aligning daughter cells with the external force field...
  17. doi The Aurora kinase family in cell division and cancer
    Gerben Vader
    Laboratory of Experimental Oncology, Department of Medical Oncology, University Medical Center Utrecht, Stratenum 2 125, Universiteitsweg 100, 3584 CG Utrecht, The Netherlands
    Biochim Biophys Acta 1786:60-72. 2008
    ....
  18. pmc The cortical protein Lte1 promotes mitotic exit by inhibiting the spindle position checkpoint kinase Kin4
    Daniela Trinca Bertazzi
    DKFZ ZMBH Alliance, German Cancer Research Center, 69120 Heidelberg, Germany
    J Cell Biol 193:1033-48. 2011
    ..This Lte1-mediated exclusion of Kin4 from the dSPB is essential for proper mitotic exit of cells with a correctly aligned spindle. Therefore, Lte1 promotes mitotic exit by inhibiting Kin4 activity at the dSPB...
  19. pmc Making the Auroras glow: regulation of Aurora A and B kinase function by interacting proteins
    Mar Carmena
    Wellcome Trust Centre for Cell Biology, University of Edinburgh, Michael Swann Building, King s Buildings, Edinburgh, Scotland, UK
    Curr Opin Cell Biol 21:796-805. 2009
    ..This review will focus on how interacting proteins make this functional diversity possible by targeting the kinases to different subcellular locations and regulating their activity...
  20. doi Spindle orientation bias in gut epithelial stem cell compartments is lost in precancerous tissue
    Aaron J Quyn
    Cell and Developmental Biology, University of Dundee, Dundee, DD1 5EH, UK
    Cell Stem Cell 6:175-81. 2010
    ..This loss correlates with cell shape changes specifically in the stem cell compartment. Our data suggest that loss of asymmetric division in stem cells might contribute to the oncogenic effect of Apc mutations in gut epithelium...
  21. ncbi The cellular geography of aurora kinases
    Mar Carmena
    Wellcome Trust Centre for Cell Biology, Institute for Cell and Molecular Biology, Kings Buildings, University of Edinburgh, Mayfield Road, Edinburgh EH9 3JR, Scotland, UK
    Nat Rev Mol Cell Biol 4:842-54. 2003
  22. pmc Multipolar spindle pole coalescence is a major source of kinetochore mis-attachment and chromosome mis-segregation in cancer cells
    William T Silkworth
    Virginia Tech, Department of Biological Sciences, Blacksburg, VA, USA
    PLoS ONE 4:e6564. 2009
    ..We propose this spindle pole coalescence mechanism as a major contributor to chromosome instability in cancer cells...
  23. pmc Cep152 acts as a scaffold for recruitment of Plk4 and CPAP to the centrosome
    Onur Cizmecioglu
    Cell Cycle Control and Carcinogenesis, German Cancer Research Center DKFZ, 69120 Heidelberg, Germany
    J Cell Biol 191:731-9. 2010
    ..Our results suggest that Cep152 recruits Plk4 and CPAP to the centrosome to ensure a faithful centrosome duplication process...
  24. pmc Cdc42 controls spindle orientation to position the apical surface during epithelial morphogenesis
    Aron B Jaffe
    Cell Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA
    J Cell Biol 183:625-33. 2008
    ..We conclude that Cdc42 regulates epithelial tissue morphogenesis by controlling spindle orientation during cell division...
  25. pmc Par3 controls epithelial spindle orientation by aPKC-mediated phosphorylation of apical Pins
    Yi Hao
    Department of Cell Biology, University of Virginia School of Medicine, Charlottesville, VA 22908, USA
    Curr Biol 20:1809-18. 2010
    ..Defects in signaling through Cdc42 and atypical protein kinase C (aPKC) also cause spindle misorientation. When epithelial cysts are grown in 3D cultures, misorientation creates multiple lumens...
  26. pmc Pharmacologic inhibition of the anaphase-promoting complex induces a spindle checkpoint-dependent mitotic arrest in the absence of spindle damage
    Xing Zeng
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Cancer Cell 18:382-95. 2010
    ..We propose that mutual antagonism between the APC and the SAC yields a positive feedback loop that amplifies the ability of TAME to induce mitotic arrest...
  27. ncbi Mitotic kinases as regulators of cell division and its checkpoints
    E A Nigg
    Max Planck Institute for Biochemistry, Department of Cell Biology, Am Klopferspitz 18a, D 82152 Martinsried, Germany
    Nat Rev Mol Cell Biol 2:21-32. 2001
    ..Here, I give an overview of the many mitotic kinases that regulate cell division and the fidelity of chromosome transmission...
  28. pmc Mouse inscuteable induces apical-basal spindle orientation to facilitate intermediate progenitor generation in the developing neocortex
    Maria Pia Postiglione
    Institute of Molecular Biotechnology of the Austrian Academy of Sciences IMBA, Dr Bohr Gasse 3 5, 1030 Vienna, Austria
    Neuron 72:269-84. 2011
    ..Our results indicate that the orientation of progenitor cell divisions is important for correct lineage specification in the developing mammalian brain...
  29. pmc KNL1/Spc105 recruits PP1 to silence the spindle assembly checkpoint
    Jessica S Rosenberg
    Laboratory of Chromosome and Cell Biology, The Rockefeller University, New York, NY 10065, USA
    Curr Biol 21:942-7. 2011
    ..We propose that the PP1-Spc105 interaction enables local regulation of dynamic phosphorylation and dephosphorylation at the kinetochore to couple microtubule attachment and SAC silencing...
  30. ncbi Cep97 and CP110 suppress a cilia assembly program
    Alexander Spektor
    Department of Pathology and NYU Cancer Institute, New York University School of Medicine, Smilow Research Center, 522 First Avenue, New York, NY 10016, USA
    Cell 130:678-90. 2007
    ..Identification of Cep97 and other genes involved in regulation of cilia assembly may accelerate our understanding of human ciliary diseases, including renal disease and retinal degeneration...
  31. ncbi A bouquet makes ends meet
    H Scherthan
    Department of Human Biology and Human Genetics, University of Kaiserslautern, D 67653 Kaiserslautern, Germany
    Nat Rev Mol Cell Biol 2:621-7. 2001
    ..There have been many hypotheses about its role in the pairing of meiotic chromosomes, but until recently these have been difficult to test...
  32. pmc LGN regulates mitotic spindle orientation during epithelial morphogenesis
    Zhen Zheng
    Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, GA 30912, USA
    J Cell Biol 189:275-88. 2010
    ..The normal apical exclusion of LGN during mitosis appears to be mediated by atypical PKC. Thus, cell polarization-mediated spatial restriction of spindle orientation determinants is critical for epithelial morphogenesis...
  33. pmc Ric-8A and Gi alpha recruit LGN, NuMA, and dynein to the cell cortex to help orient the mitotic spindle
    Geoffrey E Woodard
    B Cell Molecular Immunology Section, Laboratory of Immunoregulation, National Institute of Allergy and Infectious Disease, 10 Center Drive, MSC 1888, Bethesda, MD 20892 1752, USA
    Mol Cell Biol 30:3519-30. 2010
    ..These data indicate that Ric-8A signaling leads to assembly of a cortical signaling complex that functions to orient the mitotic spindle...
  34. pmc Aurora kinases and protein phosphatase 1 mediate chromosome congression through regulation of CENP-E
    Yumi Kim
    Ludwig Institute for Cancer Research, University of California, San Diego, La Jolla, CA 92093, USA
    Cell 142:444-55. 2010
    ....
  35. ncbi Structure of a Survivin-Borealin-INCENP core complex reveals how chromosomal passengers travel together
    A Arockia Jeyaprakash
    Max Planck Institute of Biochemistry, Am Klopferspitz 18, D 82152 Martinsried, Germany
    Cell 131:271-85. 2007
    ..Association of the core "passenger" proteins creates a single structural unit, whose composite molecular surface presents conserved residues essential for central spindle and midbody localization...
  36. pmc Robust control of mitotic spindle orientation in the developing epidermis
    Nicholas D Poulson
    Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA
    J Cell Biol 191:915-22. 2010
    ..These data have uncovered two important regulatory points controlling ACD in the epidermis and allow a framework for analysis of how external cues control this important choice...
  37. ncbi Control of apoptosis and mitotic spindle checkpoint by survivin
    F Li
    Boyer Center for Molecular Medicine, Department of Pathology, Yale University School of Medicine, New Haven, Connecticut 06536, USA
    Nature 396:580-4. 1998
    ..The overexpression of survivin in cancer may overcome this apoptotic checkpoint and favour aberrant progression of transformed cells through mitosis...
  38. pmc Human mutations in NDE1 cause extreme microcephaly with lissencephaly [corrected]
    Fowzan S Alkuraya
    Department of Genetics, King Faisal Specialist Hospital and Research Center, Riyadh 11211, Saudi Arabia
    Am J Hum Genet 88:536-47. 2011
    ....
  39. doi CLASPs prevent irreversible multipolarity by ensuring spindle-pole resistance to traction forces during chromosome alignment
    Elsa Logarinho
    Instituto de Biologia Molecular e Celular, Universidade do Porto, Rua do Campo Alegre 823, 4150 180 Porto, Portugal
    Nat Cell Biol 14:295-303. 2012
    ..We propose that CLASPs and ninein confer spindle-pole resistance to traction forces exerted during chromosome congression, thereby preventing irreversible spindle multipolarity and aneuploidy...
  40. pmc Dynamic maintenance of asymmetric meiotic spindle position through Arp2/3-complex-driven cytoplasmic streaming in mouse oocytes
    Kexi Yi
    Stowers Institute for Medical Research, 1000 East 50th Street, Kansas City, Missouri 64110, USA
    Nat Cell Biol 13:1252-8. 2011
    ..Thus, the asymmetric MII spindle position is dynamically maintained as a result of balanced forces governed by the Arp2/3 complex...
  41. doi MAP4 and CLASP1 operate as a safety mechanism to maintain a stable spindle position in mitosis
    Catarina P Samora
    Centre for Mechanochemical Cell Biology, Warwick Medical School, University of Warwick, Coventry, CV4 7AL, UK
    Nat Cell Biol 13:1040-50. 2011
    ..These findings have wide biological importance, because spindle positioning is essential during embryogenesis and stem-cell homeostasis...
  42. pmc Cdk5rap2 exposes the centrosomal root of microcephaly syndromes
    Timothy L Megraw
    Department of Biomedical Sciences, Florida State University, College of Medicine, Tallahassee, USA
    Trends Cell Biol 21:470-80. 2011
    ..We integrate the impact of centriole replication defects with the functions of Cdk5rap2 and other MCPH proteins, propose mechanisms for progenitor loss in MCPH, and discuss links to two other microcephaly syndromes...
  43. doi Aurora B regulates formin mDia3 in achieving metaphase chromosome alignment
    Lina Cheng
    Department of Pathology and Cell Biology, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA
    Dev Cell 20:342-52. 2011
    ..These findings reveal a key role for mDia3 and its regulation by Aurora B phosphorylation in achieving proper stable kinetochore microtubule attachment...
  44. pmc Telomere-led bouquet formation facilitates homologous chromosome pairing and restricts ectopic interaction in fission yeast meiosis
    O Niwa
    Kazusa DNA Research Institute, 1532 3 Yana, Kisarazu, Chiba 292 0812, Japan
    EMBO J 19:3831-40. 2000
    ..Thus, telomere-led chromosome organization facilitates homologous pairing and also restricts irregular chromosome pairing during meiosis...
  45. ncbi Identification of a vertebrate sister-chromatid separation inhibitor involved in transformation and tumorigenesis
    H Zou
    Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Science 285:418-22. 1999
    ..The oncogenic nature of increased expression of vSecurin may result from chromosome gain or loss, produced by errors in chromatid separation...
  46. pmc Integrin-mediated adhesion orients the spindle parallel to the substratum in an EB1- and myosin X-dependent manner
    Fumiko Toyoshima
    Department of Cell and Developmental Biology, Graduate School of Biostudies, Kyoto University, Sakyo ku, Kyoto, Japan
    EMBO J 26:1487-98. 2007
    ..Our results thus uncover the existence of a mechanism that orients the spindle parallel to the cell-substrate adhesion plane, and identify crucial factors involved in this novel mechanism...
  47. ncbi SIN and the art of splitting the fission yeast cell
    Andrea Krapp
    Cell Cycle Control Laboratory, Swiss Institute for Experimental Cancer Research ISREC, 1066 Epalinges, Switzerland
    Curr Biol 14:R722-30. 2004
    ..Flp1p, the S. pombe ortholog of Cdc14p, is not essential for mitotic exit, but may contribute to an orderly mitosis-G1 transition by regulating the destruction of the mitotic inducer cdc25p...
  48. pmc Protein phosphatase 6 regulates mitotic spindle formation by controlling the T-loop phosphorylation state of Aurora A bound to its activator TPX2
    Kang Zeng
    University of Liverpool, Cancer Research Centre, Liverpool L3 9TA, England, UK
    J Cell Biol 191:1315-32. 2010
    ..These results demonstrate a hitherto unappreciated role for PP6 as the T-loop phosphatase regulating Aurora A activity during spindle formation and suggest the general importance of this form of regulation...
  49. ncbi Two-hybrid search for proteins that interact with Sad1 and Kms1, two membrane-bound components of the spindle pole body in fission yeast
    F Miki
    Kazusa DNA Research Institute, 292 0818, Kisarazu, Chiba, Japan
    Mol Genet Genomics 270:449-61. 2004
    ..We found that none of the newly identified genes tested were necessary for centromere/telomere clustering...
  50. pmc Constitutive Mad1 targeting to kinetochores uncouples checkpoint signalling from chromosome biorientation
    Maria Maldonado
    Laboratory of Chemistry and Cell Biology, The Rockefeller University, New York, New York 10065, USA
    Nat Cell Biol 13:475-82. 2011
    ....
  51. pmc The augmin complex plays a critical role in spindle microtubule generation for mitotic progression and cytokinesis in human cells
    Ryota Uehara
    Institute for Advanced Research, Nagoya University, Furo cho, Chikusa ku, Nagoya 464 8601, Japan
    Proc Natl Acad Sci U S A 106:6998-7003. 2009
    ..These results demonstrate that MT generation mediated by augmin and gamma-TuRC is critical for chromosome segregation and cytokinesis in human cells...
  52. pmc Identification of an Aurora-A/PinsLINKER/Dlg spindle orientation pathway using induced cell polarity in S2 cells
    Christopher A Johnston
    Institute of Neuroscience, University of Oregon, Eugene, OR 97403, USA
    Cell 138:1150-63. 2009
    ..This "induced cortical polarity" assay is suitable for rapid identification of the proteins, domains, and amino acids regulating spindle orientation or cell polarity...
  53. pmc Mitotic control of kinetochore-associated dynein and spindle orientation by human Spindly
    Ying Wai Chan
    Department of Cell Biology, Max Planck Institute of Biochemistry, D 82152 Martinsried, Germany
    J Cell Biol 185:859-74. 2009
    ..Collectively, our data reveal hSpindly-mediated dynein functions and highlight a critical role of KT dynein in spindle orientation...
  54. pmc Human chromokinesin KIF4A functions in chromosome condensation and segregation
    Manjari Mazumdar
    National Cancer Institute, National Institutes of Health, Bldg 41, Rm B 507, 41 Library Dr, Bethesda, MD 20892, USA
    J Cell Biol 166:613-20. 2004
    ..Our results provide functional evidence that human KIF4A is a novel component of the chromosome condensation and segregation machinery functioning in multiple steps of mitotic division...
  55. ncbi Validating Aurora B as an anti-cancer drug target
    Fiona Girdler
    Faculty of Life Sciences, Michael Smith Building, Oxford Road, University of Manchester, Manchester, M13 9PT, UK
    J Cell Sci 119:3664-75. 2006
    ..Because small molecule-mediated inhibition of Aurora A and Aurora B yields distinct phenotypes, our observations indicate that the Auroras may present two avenues for anti-cancer drug discovery...
  56. pmc Aurora B couples chromosome alignment with anaphase by targeting BubR1, Mad2, and Cenp-E to kinetochores
    Claire Ditchfield
    School of Biological Sciences, University of Manchester, 2 205 Stopford Building, Oxford Rd, Manchester M13 9PT, UK
    J Cell Biol 161:267-80. 2003
    ..Together, these results suggest that by targeting checkpoint proteins to kinetochores, Aurora B couples chromosome alignment with anaphase onset...
  57. ncbi Nuclear transport and the mitotic apparatus: an evolving relationship
    Richard Wozniak
    Department of Cell Biology, University of Alberta, Edmonton, AB, T6G 2H7, Canada
    Cell Mol Life Sci 67:2215-30. 2010
    ..In this review, we will discuss this evolving area of study and emerging hypotheses that propose key roles for components of the nuclear transport apparatus in mitotic progression...
  58. doi Targeting of the RhoGEF Ect2 to the equatorial membrane controls cleavage furrow formation during cytokinesis
    Kuan Chung Su
    Cell Division and Aneuploidy Laboratory, Cancer Research UK London Research Institute, Clare Hall Laboratories, Blanche Lane, South Mimms, Hertfordshire EN6 3LD, UK
    Dev Cell 21:1104-15. 2011
    ..We propose that targeting of Ect2 to the equatorial membrane represents a key step in the delivery of the cytokinetic signal to the cortex...
  59. pmc Katanin contributes to interspecies spindle length scaling in Xenopus
    Rose Loughlin
    Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA
    Cell 147:1397-407. 2011
    ..Thus, mechanisms have evolved in different species to scale spindle size and coordinate regulation of multiple MT populations in order to generate a robust steady-state structure...
  60. pmc Arp2/3 complex regulates asymmetric division and cytokinesis in mouse oocytes
    Shao Chen Sun
    Department of Animal Sciences, Chungbuk National University, Cheongju, Republic of Korea
    PLoS ONE 6:e18392. 2011
    ..Our data suggest that the Arp2/3 complex probably regulates oocyte polarization through its effect on spindle migration, asymmetric division and cytokinesis during mouse oocyte meiotic maturation...
  61. ncbi CIK1: a developmentally regulated spindle pole body-associated protein important for microtubule functions in Saccharomyces cerevisiae
    B D Page
    Department of Biology, Yale University, New Haven, Connecticut 06511
    Genes Dev 6:1414-29. 1992
    ....
  62. pmc Exploring the functional interactions between Aurora B, INCENP, and survivin in mitosis
    Reiko Honda
    Department of Cell Biology, Max Planck Institute for Biochemistry, D 82152 Martinsried, Germany
    Mol Biol Cell 14:3325-41. 2003
    ..A nonphosphorylatable mutant (TSS893-895AAA) was a poor activator of Aurora B, demonstrating that INCENP phosphorylation is important for kinase activation...
  63. ncbi Formin-2 is required for spindle migration and for the late steps of cytokinesis in mouse oocytes
    Julien Dumont
    Equipe Divisions Méiotiques chez la souris, UMR7622, CNRS, Universite Pierre et Marie Curie Paris 6, Bat C, 5e, 9 Quai Saint Bernard 75005, Paris, France
    Dev Biol 301:254-65. 2007
    ..We show here that attempts of cytokinesis in these oocytes abort due to phospho-myosin II mislocalization...
  64. pmc Neuroepithelial stem cell proliferation requires LIS1 for precise spindle orientation and symmetric division
    Jessica Yingling
    Departments of Pediatrics and Medicine, Center for Human Genetics and Genomics, UCSD School of Medicine, 9500 Gilman Drive, La Jolla, CA 92098 0627, USA
    Cell 132:474-86. 2008
    ..Thus, control of symmetric division, essential for neuroepithelial stem cell proliferation, is mediated through spindle orientation determined via LIS1/NDEL1/dynein-mediated cortical microtubule capture...
  65. ncbi Microtubule nucleation: gamma-tubulin and beyond
    Christiane Wiese
    Department of Biochemistry, University of Wisconsin Madison, 433 Babcock Drive, Madison, WI 53706, USA
    J Cell Sci 119:4143-53. 2006
    ....
  66. pmc Condensin regulates the stiffness of vertebrate centromeres
    Susana A Ribeiro
    Wellcome Trust Centre for Cell Biology, Institute of Cell and Molecular Biology, University of Edinburgh, Edinburgh EH9 3JR, United Kingdom
    Mol Biol Cell 20:2371-80. 2009
    ..Loss of stiffness caused by condensin-depletion produces abnormal uncoordinated sister kinetochore movements, leads to an increase in Mad2(+) kinetochores near the metaphase plate and delays anaphase onset...
  67. ncbi Mechanisms of asymmetric cell division: flies and worms pave the way
    PIERRE GONCZY
    Swiss Institute for Experimental Cancer Research ISREC, Swiss Federal Institute of Technology EPFL, School of Life Sciences, Lausanne, Switzerland
    Nat Rev Mol Cell Biol 9:355-66. 2008
    ..Lessons learned from these two model organisms also apply to cells that divide asymmetrically in other metazoans, such as self-renewing stem cells in mammals...
  68. doi Phosphorylation-dependent protein interactions at the spindle midzone mediate cell cycle regulation of spindle elongation
    Anton Khmelinskii
    Zentrum fur Molekulare Biologie der Universitat Heidelberg, DKFZ ZMBH Allianz, Im Neuenheiemer Feld 282, Heidelberg 69117, Germany
    Dev Cell 17:244-56. 2009
    ..Our results reveal that sliding forces at the midzone are activated by separase and explain how spindle elongation is triggered with anaphase entry...
  69. ncbi Self-organization of MTOCs replaces centrosome function during acentrosomal spindle assembly in live mouse oocytes
    Melina Schuh
    Gene Expression Unit, European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    Cell 130:484-98. 2007
    ..Finally, a stable barrel-shaped acentrosomal metaphase spindle with oscillating chromosomes and astral-like microtubules forms that surprisingly exhibits key properties of a centrosomal spindle...
  70. doi Regulation of centrosome separation in yeast and vertebrates: common threads
    Hong Hwa Lim
    Institute of Molecular and Cell Biology, Agency for Science, Technology and Research A STAR, Proteos, 138673 Singapore
    Trends Cell Biol 19:325-33. 2009
    ..Here, we review recent studies that uncover new players and provide a greater understanding of the regulation of centrosome (or SPB) separation...
  71. pmc DNA relaxation dynamics as a probe for the intracellular environment
    J K Fisher
    Department of Biomedical Engineering, University of North Carolina, Chapel Hill, NC 27599, USA
    Proc Natl Acad Sci U S A 106:9250-5. 2009
    ..As a result, the technique described herein may be used as a biophysical strategy to probe the intranuclear environment...
  72. ncbi Asymmetric loading of Kar9 onto spindle poles and microtubules ensures proper spindle alignment
    Dimitris Liakopoulos
    Institute of Biochemistry, Swiss Federal Institute of Technology, ETH Hoenggerberg, HPM, CH 8093 Zurich, Switzerland
    Cell 112:561-74. 2003
    ..In turn, asymmetric distribution of Kar9 ensures that only one pole orients toward the bud. Our results indicate that Cdk1-dependent spindle asymmetry ensures proper alignment of the mitotic spindle with the cell division axis...
  73. ncbi Allocation of gamma-tubulin between oocyte cortex and meiotic spindle influences asymmetric cytokinesis in the mouse oocyte
    Susan L Barrett
    Program in Cellular, Molecular and Developmental Biology, Tufts University School of Medicine, Boston, MA 02111, USA
    Biol Reprod 76:949-57. 2007
    ..Together, these studies reinforce the notion that limiting gamma-tubulin availability during meiotic maturation ensures coordination of karyokinesis and cytokinesis and conservation of gamma-tubulin as an embryonic reserve...
  74. pmc N-terminal regions of Mps1 kinase determine functional bifurcation
    Yasuhiro Araki
    Zentrum fur Molekulare Biologie der Universitat Heidelberg, Deutsches Krebsforschungszentrum ZMBH Allianz, 69120 Heidelberg, Germany
    J Cell Biol 189:41-56. 2010
    ..Mps1 phosphorylation of Cdc31 at the conserved T110 residue controls substrate binding to Kar1 protein. These findings explain the multiple SPB duplication defects of mps1 mutants on a molecular level...
  75. pmc Pericentromeric sister chromatid cohesion promotes kinetochore biorientation
    Tessie M Ng
    Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
    Mol Biol Cell 20:3818-27. 2009
    ..Taken together, these data reveal a specific role for pericentromeric linkage in ensuring kinetochore biorientation...
  76. pmc The human RNA polymerase II-associated factor 1 (hPaf1): a new regulator of cell-cycle progression
    Nicolas Moniaux
    Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, Nebraska, USA
    PLoS ONE 4:e7077. 2009
    ..Further, the yeast homolog of hPaf1 has a role in regulating the expression of a subset of genes involved in the cell-cycle. We therefore investigated the role of hPaf1 during progression of the cell-cycle...
  77. pmc The Nup107-160 complex and gamma-TuRC regulate microtubule polymerization at kinetochores
    Ram Kumar Mishra
    Laboratory of Gene Regulation and Development, NICHD NIH, Bethesda, MD 20892, USA
    Nat Cell Biol 12:164-9. 2010
    ....
  78. pmc Ska3 is required for spindle checkpoint silencing and the maintenance of chromosome cohesion in mitosis
    John R Daum
    Cell Cycle and Cancer Biology Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK 73104, USA
    Curr Biol 19:1467-72. 2009
    ..We suggest that this network plays a major role in silencing the spindle checkpoint when chromosomes are aligned at metaphase to allow timely anaphase onset and mitotic exit...
  79. pmc Regulation of cytokinesis by Rho GTPase flux
    Ann L Miller
    Department of Zoology, University of Wisconsin Madison, Madison, WI 53706, USA
    Nat Cell Biol 11:71-7. 2009
    ....
  80. ncbi Inhibition of casein kinase I delta alters mitotic spindle formation and induces apoptosis in trophoblast cells
    Martin Stöter
    Department of Visceral and Transplantation Surgery, University of Ulm, Ulm, Germany
    Oncogene 24:7964-75. 2005
    ..Our findings indicate that CKIdelta plays an important role in the mitotic progression and in the survival of cells of trophoblast origin. Therefore, IC261 could provide a new tool in treating choriocarcinomas...
  81. pmc SAS-4 is recruited to a dynamic structure in newly forming centrioles that is stabilized by the gamma-tubulin-mediated addition of centriolar microtubules
    Alexander Dammermann
    Department of Cellular and Molecular Medicine, Ludwig Institute for Cancer Research, University of California, San Diego, La Jolla, CA 92093, USA
    J Cell Biol 180:771-85. 2008
    ..Such a mechanism may help restrict new centriole assembly to the vicinity of preexisting parent centrioles that recruit PCM...
  82. pmc Force and length in the mitotic spindle
    Sophie Dumont
    Department of Systems Biology, Harvard Medical School, Boston, MA 02115, USA
    Curr Biol 19:R749-61. 2009
    ..We then discuss models for force integration and spindle length determination. We also emphasize key missing data that notably include absolute values of forces and how they vary as a function of position within the spindle...
  83. ncbi Neurogenesis and asymmetric cell division
    Weimin Zhong
    Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT, USA
    Curr Opin Neurobiol 18:4-11. 2008
    ..Here we review some recent developments involving Drosophila and mammalian neural progenitor cells, highlighting some similarities and differences in the mechanisms that regulate their divisions during neurogenesis...
  84. pmc Requirement for Nudel and dynein for assembly of the lamin B spindle matrix
    Li Ma
    Laboratory of Molecular Cell Biology and Center of Cell Signaling, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China
    Nat Cell Biol 11:247-56. 2009
    ..We suggest that Nudel regulates microtubule organization in part by facilitating assembly of the lamin B spindle matrix in a dynein-dependent manner...
  85. pmc Ase1/Prc1-dependent spindle elongation corrects merotely during anaphase in fission yeast
    Thibault Courtheoux
    Universite de Toulouse, Laboratoire de Biologie Cellulaire et Moléculaire du Controle de la Prolifération UMR5088, F 31062 Toulouse, France
    J Cell Biol 187:399-412. 2009
    ....
  86. ncbi Chromosome segregation machinery and cancer
    Kozo Tanaka
    Institute of Development, Aging and Cancer, Tohoku University, Sendai, Japan
    Cancer Sci 100:1158-65. 2009
    ..Understanding these mechanisms may lead to the discovery of new and improved anticancer therapies...
  87. pmc Towards building a chromosome segregation machine
    Kerry Bloom
    Department of Biology, 622 Fordham Hall, CB3280, University of North Carolina at Chapel Hill, North Carolina 27599, USA
    Nature 463:446-56. 2010
    ..But when it comes to packaging and segregating a genome, the mechanisms are only beginning to be understood and are often as variable as the organisms in which they are studied...
  88. pmc Contrasting models for kinetochore microtubule attachment in mammalian cells
    Bruce F McEwen
    Wadsworth Center, New York State Department of Health, Albany, NY 12201, USA
    Cell Mol Life Sci 67:2163-72. 2010
    ..It is currently unclear whether kinetochores control and coordinate the dynamics of individual kinetochore microtubules...
  89. ncbi Sister chromatid tension and the spindle assembly checkpoint
    Luigi Nezi
    Department of Experimental Oncology, European Institute of Oncology, Milan, Italy
    Curr Opin Cell Biol 21:785-95. 2009
    ..We discuss how tension-sensitive phenomena, such as attachment correction and stabilization, relate to the SAC and we speculate on the existence of a single pathway linking error correction and SAC activation...
  90. ncbi Rotation of meiotic spindle is controlled by microfilaments in mouse oocytes
    Zi Yu Zhu
    State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China
    Biol Reprod 68:943-6. 2003
    ..These results indicate that spindle rotation is essential for polar body extrusion; it is the microfilaments that play a crucial role in regulating rotation of the meiotic spindle...
  91. doi A new model for asymmetric spindle positioning in mouse oocytes
    Melina Schuh
    European Molecular Biology Laboratory EMBL, Gene Expression Unit, Heidelberg, Germany
    Curr Biol 18:1986-92. 2008
    ..Based on these results, we propose the first mechanistic model for asymmetric spindle positioning in mammalian oocytes and validate five of its key predictions experimentally...
  92. doi Quantitative analysis of the human spindle phosphoproteome at distinct mitotic stages
    Rainer Malik
    Max Planck Institute of Biochemistry, Department of Cell Biology, Am Klopferspitz 18, D 82152 Martinsried, Germany
    J Proteome Res 8:4553-63. 2009
    ..Taken together, our results constitute a large quantitative data resource of phosphorylation abundances at distinct mitotic stages and they provide insight into the systems properties of phosphorylation dynamics during mitosis...
  93. pmc DNA topoisomerase II is a determinant of the tensile properties of yeast centromeric chromatin and the tension checkpoint
    Tariq H Warsi
    Department of Cell Biology and Neuroscience, University of California, Riverside, Riverside, CA 92521, USA
    Mol Biol Cell 19:4421-33. 2008
    ..In sum, Top2 seems to play a novel role in CEN compaction that is distinct from decatenation. Perturbations to this function may allow weakened kinetochores to stretch CENs in a manner that mimics tension or evades Ipl1 surveillance...
  94. pmc Design features of a mitotic spindle: balancing tension and compression at a single microtubule kinetochore interface in budding yeast
    David C Bouck
    Department of Biology, University of North Carolina, Chapel Hill, North Carolina 27599 3280, USA
    Annu Rev Genet 42:335-59. 2008
    ....
  95. ncbi VHL loss causes spindle misorientation and chromosome instability
    Claudio R Thoma
    Institute of Cell Biology, ETH Zurich, 8093 Zurich, Switzerland
    Nat Cell Biol 11:994-1001. 2009
    ....
  96. ncbi Emerging cancer therapeutic opportunities by inhibiting mitotic kinases
    Ignacio Perez de Castro
    Cell Division and Cancer Group, Centro Nacional de Investigaciones Oncologicas CNIO, Melchor Fernandez Almagro 3, Madrid, Spain
    Curr Opin Pharmacol 8:375-83. 2008
    ....
  97. ncbi Mad2 is required for inhibiting securin and cyclin B degradation following spindle depolymerisation in meiosis I mouse oocytes
    Hayden A Homer
    Newcastle Fertility Centre at Life, International Centre for Life, Times Square, Newcastle upon Tyne, NE1 4EP, UK
    Reproduction 130:829-43. 2005
    ..In conclusion, when all kinetochores lack attachment and tension, mouse oocytes mount a robust Mad2-dependent meiosis I arrest which inhibits the destruction of securin and cyclin B...
  98. pmc MLN8054, a small-molecule inhibitor of Aurora A, causes spindle pole and chromosome congression defects leading to aneuploidy
    Kara Hoar
    Department of Molecular and Cellular Oncology, Millenium Pharmaceuticals Inc, Cambridge, MA 02139, USA
    Mol Cell Biol 27:4513-25. 2007
    ..Taken together, these results suggest that Aurora A inhibition kills tumor cells through the development of deleterious aneuploidy...
  99. pmc Mitotic spindle disassembly occurs via distinct subprocesses driven by the anaphase-promoting complex, Aurora B kinase, and kinesin-8
    Jeffrey B Woodruff
    Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA
    J Cell Biol 191:795-808. 2010
    ..Combinatorial inhibition of these subprocesses yielded cells with hyperstable spindle remnants and dramatic defects in cell cycle progression, establishing that rapid spindle disassembly is crucial for cell proliferation...
  100. doi Oocyte spindle proteomics analysis leading to rescue of chromosome congression defects in cloned embryos
    Zhiming Han
    Department of Biochemistry, Temple University School of Medicine, Philadelphia, PA, USA
    J Proteome Res 9:6025-32. 2010
    ..These studies are the first to employ proteomics analyses coupled to functional interventions to rescue a specific molecular defect in cloned embryos...
  101. pmc CLASP1, astrin and Kif2b form a molecular switch that regulates kinetochore-microtubule dynamics to promote mitotic progression and fidelity
    Amity L Manning
    Department of Biochemistry, Dartmouth Medical School, Hanover, NH 03766, USA
    EMBO J 29:3531-43. 2010
    ..Thus, CLASP1-astrin-Kif2b complex act as a central switch at kinetochores that defines mitotic progression and promotes fidelity by temporally regulating kMT attachments...

Research Grants100

  1. Molecular Dissection of Cytokinesis
    Michael Glotzer; Fiscal Year: 2007
    ..Furthermore, molecular dissection of ZEN-4 will enhance our understanding of the mechanism of action of microtubule motors. ..
  2. A Role for the Novel Nuclear Speckle Protein Son in Maintaining Nuclear Speckle I
    Paula Bubulya; Fiscal Year: 2009
    ..roles for Son in regulation of gene expression, repression of replication of Hepatitis B Virus, in the mitotic spindle apparatus and in anti-apoptotic response gives human disease relevance for studying Son functions...
  3. MOLECULAR ANALYSIS OF CHROMOSOME SEGREGATION IN YEAST
    Michael Snyder; Fiscal Year: 1990
    Microtubules are essential for many vital cellular processes, including formation of the mitotic spindle apparatus. The goal of our research is to identify and characterize molecular components of spindle poles, the organizing centers ..
  4. MOLECULAR ANALYSIS OF CHROMOSOME SEGREGATION IN YEAST
    Michael Snyder; Fiscal Year: 1991
    Microtubules are essential for many vital cellular processes, including formation of the mitotic spindle apparatus. The goal of our research is to identify and characterize molecular components of spindle poles, the organizing centers ..
  5. MOLECULAR ANALYSIS OF CHROMOSOME SEGREGATION IN YEAST
    Michael Snyder; Fiscal Year: 1993
    Microtubules are essential for many vital cellular processes, including formation of the mitotic spindle apparatus. The goal of our research is to identify and characterize molecular components of spindle poles, the organizing centers ..
  6. MOLECULAR ANALYSIS OF CHROMOSOME SEGREGATION IN YEAST
    Michael Snyder; Fiscal Year: 1992
    Microtubules are essential for many vital cellular processes, including formation of the mitotic spindle apparatus. The goal of our research is to identify and characterize molecular components of spindle poles, the organizing centers ..
  7. YEAST CHROMOSOME STRUCTURE, REPLICATION AND SEGREGATION
    Victoria Lundblad; Fiscal Year: 2000
    ..telomeres and position effects, centromeres and their interacting proteins, components of the mitotic spindle apparatus, microtubule motors, meiotic chromosome segregation and chromosome cohesion...
  8. Spindle Assembly Checkpoint, Chromosomal Instability, and Cancer
    Pumin Zhang; Fiscal Year: 2009
    ..that delays the segregation of sister chromatids until all chromosomes are properly attached to the mitotic spindle apparatus and aligned at the metaphase plate...
  9. Spindle Assembly Checkpoint, Chromosomal Instability, and Cancer
    Pumin Zhang; Fiscal Year: 2010
    ..that delays the segregation of sister chromatids until all chromosomes are properly attached to the mitotic spindle apparatus and aligned at the metaphase plate...
  10. Nuclear Transport Factors in Chromosomal Instability
    Jan van Deursen; Fiscal Year: 2007
    ..molecular machine that blocks the onset of anaphase until all chromosomes are properly attached to the mitotic spindle apparatus and aligned at the metaphase plate...
  11. Nuclear Transport Factors in Chromosomal Instability
    Jan van Deursen; Fiscal Year: 2006
    ..molecular machine that blocks the onset of anaphase until all chromosomes are properly attached to the mitotic spindle apparatus and aligned at the metaphase plate...
  12. Nuclear Transport Factors in Chromosomal Instability
    Jan van Deursen; Fiscal Year: 2005
    ..molecular machine that blocks the onset of anaphase until all chromosomes are properly attached to the mitotic spindle apparatus and aligned at the metaphase plate...
  13. Nuclear Transport Factors in Chromosomal Instability
    Jan van Deursen; Fiscal Year: 2003
    ..molecular machine that blocks the onset of anaphase until all chromosomes are properly attached to the mitotic spindle apparatus and aligned at the metaphase plate...
  14. Nuclear Transport Factors in Chromosomal Instability
    Jan van Deursen; Fiscal Year: 2004
    ..molecular machine that blocks the onset of anaphase until all chromosomes are properly attached to the mitotic spindle apparatus and aligned at the metaphase plate...
  15. BUBRI in Normal and Neoplastic Growth
    Jan van Deursen; Fiscal Year: 2002
    ..aneuploidy by delaying sister chromatid separation until all chromosomes are properly attached to the mitotic spindle apparatus and aligned at the metaphase plate...
  16. BUBRI in Normal and Neoplastic Growth
    Jan van Deursen; Fiscal Year: 2005
    ..aneuploidy by delaying sister chromatid separation until all chromosomes are properly attached to the mitotic spindle apparatus and aligned at the metaphase plate...
  17. BUBRI in Normal and Neoplastic Growth
    Jan van Deursen; Fiscal Year: 2004
    ..aneuploidy by delaying sister chromatid separation until all chromosomes are properly attached to the mitotic spindle apparatus and aligned at the metaphase plate...
  18. BUBRI in Normal and Neoplastic Growth
    Jan van Deursen; Fiscal Year: 2003
    ..aneuploidy by delaying sister chromatid separation until all chromosomes are properly attached to the mitotic spindle apparatus and aligned at the metaphase plate...
  19. BUBRI in Normal and Neoplastic Growth
    Jan van Deursen; Fiscal Year: 2006
    ..aneuploidy by delaying sister chromatid separation until all chromosomes are properly attached to the mitotic spindle apparatus and aligned at the metaphase plate...
  20. SIGNALING PATHWAYS THAT REGULATE MITOSIS
    THOMAS GUADAGNO; Fiscal Year: 2005
    ..2. Determine the role MAPK plays in regulating the formation and function of the mitotic spindle apparatus. 3. Determine the biochemical steps that regulate MAPK activation during mitosis...
  21. SIGNALING PATHWAYS THAT REGULATE MITOSIS
    THOMAS GUADAGNO; Fiscal Year: 2003
    ..2. Determine the role MAPK plays in regulating the formation and function of the mitotic spindle apparatus. 3. Determine the biochemical steps that regulate MAPK activation during mitosis...
  22. SIGNALING PATHWAYS THAT REGULATE MITOSIS
    THOMAS GUADAGNO; Fiscal Year: 2004
    ..2. Determine the role MAPK plays in regulating the formation and function of the mitotic spindle apparatus. 3. Determine the biochemical steps that regulate MAPK activation during mitosis...
  23. SIGNALING PATHWAYS THAT REGULATE MITOSIS
    THOMAS GUADAGNO; Fiscal Year: 2001
    ..2. Determine the role MAPK plays in regulating the formation and function of the mitotic spindle apparatus. 3. Determine the biochemical steps that regulate MAPK activation during mitosis...
  24. SIGNALING PATHWAYS THAT REGULATE MITOSIS
    THOMAS GUADAGNO; Fiscal Year: 2002
    ..2. Determine the role MAPK plays in regulating the formation and function of the mitotic spindle apparatus. 3. Determine the biochemical steps that regulate MAPK activation during mitosis...
  25. MOLECULAR AND DEVELOPMENTAL GENETICS OF DROSOPHILA
    William Gelbart; Fiscal Year: 1993
    ..e) Study the in vitro properties and in vivo function of the products of two genes, ned and nod, which belong to the kinesin superfamily and movements of microtubules in the meiotic and mitotic spindle apparatus.
  26. MOLECULAR AND DEVELOPMENTAL GENETICS OF DROSOPHILA
    Fotis Kafatos; Fiscal Year: 1991
    ..e) Study the in vitro properties and in vivo function of the products of two genes, ned and nod, which belong to the kinesin superfamily and movements of microtubules in the meiotic and mitotic spindle apparatus.
  27. Role of Mitotic Checkpoint Defects in Mouse Tumor Models
    Robert Benezra; Fiscal Year: 2006
    ..a cell prior to the metaphase to anaphase transition if all the chromosomes are not attached to the mitotic spindle apparatus. Partial loss of function mutations predisposes mammalian cells to chromosome missegregation events and ..
  28. MITOTIC CHECKPOINT GENE MAD2 IN VERTEBRATES
    Robert Benezra; Fiscal Year: 2001
    ..a cell prior to the metaphase to anaphase transition if all the chromosomes are not attached to the mitotic spindle apparatus. The main goal of the research being proposed is to determine if complete or partial loss of Mad2 ..
  29. Role of Mitotic Checkpoint Defects in Mouse Tumor Models
    Robert Benezra; Fiscal Year: 2007
    ..a cell prior to the metaphase to anaphase transition if all the chromosomes are not attached to the mitotic spindle apparatus. Partial loss of function mutations predisposes mammalian cells to chromosome missegregation events and ..
  30. MITOTIC CHECKPOINT GENE MAD2 IN VERTEBRATES
    Robert Benezra; Fiscal Year: 2003
    ..a cell prior to the metaphase to anaphase transition if all the chromosomes are not attached to the mitotic spindle apparatus. The main goal of the research being proposed is to determine if complete or partial loss of Mad2 ..
  31. MITOTIC CHECKPOINT GENE MAD2 IN VERTEBRATES
    Robert Benezra; Fiscal Year: 2002
    ..a cell prior to the metaphase to anaphase transition if all the chromosomes are not attached to the mitotic spindle apparatus. The main goal of the research being proposed is to determine if complete or partial loss of Mad2 ..
  32. Role of Mitotic Checkpoint Defects in Mouse Tumor Models
    Robert Benezra; Fiscal Year: 2009
    ..a cell prior to the metaphase to anaphase transition if all the chromosomes are not attached to the mitotic spindle apparatus. Partial loss of function mutations predisposes mammalian cells to chromosome missegregation events and ..
  33. Role of Mitotic Checkpoint Defects in Mouse Tumor Models
    ROBERT I BENEZRA; Fiscal Year: 2010
    ..a cell prior to the metaphase to anaphase transition if all the chromosomes are not attached to the mitotic spindle apparatus. Partial loss of function mutations predisposes mammalian cells to chromosome missegregation events and ..
  34. MITOTIC CHECKPOINT GENE MAD2 IN VERTEBRATES
    Robert Benezra; Fiscal Year: 2004
    ..a cell prior to the metaphase to anaphase transition if all the chromosomes are not attached to the mitotic spindle apparatus. The main goal of the research being proposed is to determine if complete or partial loss of Mad2 ..
  35. GENETIC CONTROL OF MITOTIC SPINDLE ASSEMBLY AND FUNCTION
    Daniel Burke; Fiscal Year: 2002
    ..conserved mitotic regulatory system that arrests cells when chromosomes are not properly aligned on the mitotic spindle apparatus. Mutations in spindle checkpoint genes have been implicated in genomic instability and tumor progression ..
  36. Neocortical Neurogenesis and Mitotic Spindle Dynamics
    Tarik Haydar; Fiscal Year: 2009
    ..In particular, we are studying how the movement of the mitotic spindle apparatus and expression of fate determining molecules combine to allow for the switch between symmetrical and ..
  37. GENETIC CONTROL OF MITOTIC SPINDLE ASSEMBLY AND FUNCTION
    Daniel Burke; Fiscal Year: 2005
    ..conserved mitotic regulatory system that arrests cells when chromosomes are not properly aligned on the mitotic spindle apparatus. Mutations in spindle checkpoint genes have been implicated in genomic instability and tumor progression ..
  38. Neocortical Neurogenesis and Mitotic Spindle Dynamics
    Tarik Haydar; Fiscal Year: 2007
    ..In particular, we are studying how the movement of the mitotic spindle apparatus and expression of fate determining molecules combine to allow for the switch between symmetrical and ..
  39. Neocortical Neurogenesis and Mitotic Spindle Dynamics
    Tarik Haydar; Fiscal Year: 2005
    ..In particular, we are studying how the movement of the mitotic spindle apparatus and expression of fate determining molecules combine to allow for the switch between symmetrical and ..
  40. Neocortical Neurogenesis and Mitotic Spindle Dynamics
    Tarik Haydar; Fiscal Year: 2006
    ..In particular, we are studying how the movement of the mitotic spindle apparatus and expression of fate determining molecules combine to allow for the switch between symmetrical and ..
  41. GENETIC CONTROL OF MITOTIC SPINDLE ASSEMBLY AND FUNCTION
    Daniel Burke; Fiscal Year: 2004
    ..conserved mitotic regulatory system that arrests cells when chromosomes are not properly aligned on the mitotic spindle apparatus. Mutations in spindle checkpoint genes have been implicated in genomic instability and tumor progression ..
  42. GENETIC CONTROL OF MITOTIC SPINDLE ASSEMBLY AND FUNCTION
    Daniel Burke; Fiscal Year: 2003
    ..conserved mitotic regulatory system that arrests cells when chromosomes are not properly aligned on the mitotic spindle apparatus. Mutations in spindle checkpoint genes have been implicated in genomic instability and tumor progression ..
  43. DELTAVISION RESTORATION MICROSCOPY SYSTEM MODEL 483
    Conly Rieder; Fiscal Year: 2001
    ..This Core will maintain the instrument and provide technical support to those Principal Investigators/Program Directors who are in need of low-light high- resolution 3-D fluorescent imaging. ..
  44. FORMATION,INTERACTION & FUNCTION OF SPINDLE COMPONENTS
    Conly Rieder; Fiscal Year: 2008
    ..abstract_text> ..
  45. FORMATION,INTERACTION & FUNCTION OF SPINDLE COMPONENTS
    Conly Rieder; Fiscal Year: 2007
    ..abstract_text> ..
  46. Mechanisms of asymmetric cell division during female meiosis
    FRANCIS MCNALLY; Fiscal Year: 2008
    ..A detailed molecular understanding of meiotic spindle function could thus lead to treatments that prevent birth defects and that allow stem cell therapy of human diseases. ..
  47. Mitochondria distribution in cloned pig embryos
    Heide Schatten; Fiscal Year: 2005
    ..Pilot data from this RO3 small grants program research will be used to apply for funding through the RO1 mechanism. ..
  48. Mitotic Motors in the Drosophila Embryo.
    Jonathan Scholey; Fiscal Year: 2002
    ..The results will contribute to an understanding of the basic mechanisms of mitosis and may provide insights into some of the dysfunctions in spindle action and chromosome segregation that characterize certain birth de ..
  49. STRUCTURAL ANALYSIS OF AN EUKARYOTIC CENTROMERE
    Kerry Bloom; Fiscal Year: 1991
    ..Knowledge of the factors that control the organization of the centromere and spindle may provide fundamental principles governing the molecular mechanism of cell division...
  50. FUNCTIONAL PROPERTIES OF CENTROSOMES IN SOMATIC CELLS
    Alexey Khodjakov; Fiscal Year: 2000
    ..g., as cancer, Alzheimer's, arthritis, etc.) ..
  51. Gene networks specifying cell lineages in a polychaete
    Bruce Bowerman; Fiscal Year: 2007
    ..Newly discovered and conserved loci will also be studied in C. elegans and Drosophila. [unreadable] [unreadable]..
  52. Dynamics and Mechanics of Mitosis in Drosophila
    Jonathan Scholey; Fiscal Year: 2006
    ..We aim to understand how the mitotic spindle works as a machine and thus to provide insights into how defects in its action can give rise to genomic instability, cancer and birth defects. ..
  53. Dynamics and Mechanics of Mitosis in Drosophila
    Jonathan Scholey; Fiscal Year: 2007
    ..We aim to understand how the mitotic spindle works as a machine and thus to provide insights into how defects in its action can give rise to genomic instability, cancer and birth defects. ..
  54. Cytokinesis and the Cytoskeleton in C. elegans Embryos
    Bruce Bowerman; Fiscal Year: 2004
    ....
  55. Metotic Spindle Assembly and Aneuploidy in Mammals
    Duane Compton; Fiscal Year: 2003
    ..abstract_text> ..
  56. Microtubule Motors and Chemosensory Behavior
    Jonathan Scholey; Fiscal Year: 2002
    ..elegans. Collectively, these studies will improve our understanding of the role of MT-based transport in the formation and function of neurons and in the control of the behavior of a primitive metazoan. ..
  57. Microtubule based Transport in C. elegans Neurons
    Jonathan Scholey; Fiscal Year: 2004
    ..elegans. Collectively, these studies will improve our understanding of the role of MT-based transport in the formation and function of neurons and in the control of the behavior of a primitive metazoan. ..
  58. Cytokinesis and the Cytoskeleton in C. elegans Embryos
    Bruce Bowerman; Fiscal Year: 2003
    ....
  59. CHROMOSOME AND SPINDLE DYNAMICS IN YEAST
    Kerry Bloom; Fiscal Year: 2003
    ..Our approach entails chromosome engineering, analysis of chromosome and protein dynamics by quantitative fluorescence microscopy and genetic manipulation of kinetochore components. ..
  60. FUNCTIONAL PROPERTIES OF CENTROSOMES IN SOMATIC CELLS
    Alexey Khodjakov; Fiscal Year: 2009
    ..Since centrosome abnormalities are a hallmark of malignant tumors, the knowledge obtained in these studies will be useful for designing new strategies for the treatment of cancers. ..
  61. Intrinsic Spindle Mechanisms to Insure Bipolarity
    JESSE GATLIN; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  62. Intrinsic Spindle Mechanisms to Insure Bipolarity
    JESSE GATLIN; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  63. MARCKS Phosphorylation in Growth Cone Signaling
    JESSE GATLIN; Fiscal Year: 2004
    ..Functional consequences of aberrant PKC signaling will be determined by observing the turning response of growth cones from DRG neurons expressing a dominant-negative, phosphorylation-deficient MARCKS mutant. ..
  64. Molecular Dissection of Cytokinesis
    Michael Glotzer; Fiscal Year: 2008
    ..Furthermore, molecular dissection of ZEN-4 will enhance our understanding of the mechanism of action of microtubule motors. ..
  65. Molecular Dissection of Cytokinesis
    Michael Glotzer; Fiscal Year: 2009
    ..Furthermore, molecular dissection of ZEN-4 will enhance our understanding of the mechanism of action of microtubule motors. ..
  66. Positioning the plane of cell division during cytokinesis
    Michael Glotzer; Fiscal Year: 2008
    ..Progress in this area could improve our understanding of how certain cancers develop and could identify targets for anti-tumor therapies. ..
  67. Spatial and temporal control of cell behavior with a genetically-encoded photoswi
    Michael Glotzer; Fiscal Year: 2009
    ..This strategy is genetically encoded and does not require exogenous cofactors, therefore it will be suitable for use in a wide range of cellular and developmental contexts as well as biochemical studies. ..
  68. Molecular Dissection of Cytokinesis
    Michael Glotzer; Fiscal Year: 2006
    ..Furthermore, molecular dissection of ZEN-4 will enhance our understanding of the mechanism of action of microtubule motors. [unreadable] [unreadable] [unreadable]..
  69. Positioning the plane of cell division during cytokinesis
    Michael Glotzer; Fiscal Year: 2009
    ..Progress in this area could improve our understanding of how certain cancers develop and could identify targets for anti-tumor therapies. ..
  70. Preventive of Bile Acid-Induced Colonic Oxidative DNA Damage
    Claire Payne; Fiscal Year: 2008
    ..129 mice) developed in our laboratory; 2) NOS-/- k.o./DOC mouse model; 3) arginine-enhanced colon tumor ApcMin/+ mouse model developed in our laboratory; 4) the AOM mouse model of colon carcinogenesis using A/J mice. ..
  71. Single-molecule analysis of kinesin motors in live cells
    KRISTEN VERHEY; Fiscal Year: 2008
    ..Understanding how molecular motors function in cells will provide important new targets for therapies aimed at these diseases. ..
  72. Molecular Dissection of the "Pacman-Flux" Machinery Used to Move Chromosomes
    DAVID JAMES SHARP; Fiscal Year: 2010
    ..An understanding of how this process occurs normally should provide insights into the molecular etiology of these diseases and suggest therapeutic strategies for their treatment. ..
  73. Mechanisms of Spindle Assembly
    Claire Walczak; Fiscal Year: 2009
    ....
  74. STRUCTURAL ANALYSIS OF AN EUKARYOTIC CENTROMERE
    Kerry Bloom; Fiscal Year: 1992
    ..Knowledge of the factors that control the organization of the centromere and spindle may provide fundamental principles governing the molecular mechanism of cell division...
  75. Asymmetric cell division in the C elegans embryo
    Robert Goldstein; Fiscal Year: 2004
    ..We expect that we will learn important new information about how cells divide asymmetrically that will suggest mechanisms and guide studies in mammals, including humans. ..
  76. Gamma tubulin ring complex in microtubule nucleation
    Yixian Zheng; Fiscal Year: 2003
    ..abstract_text> ..
  77. Microtubule Motor-Mechanisms of Chromosome Movements
    David Sharp; Fiscal Year: 2004
    ..abstract_text> ..
  78. Asymmetric cell division in the C elegans embryo
    Robert Goldstein; Fiscal Year: 2003
    ..We expect that we will learn important new information about how cells divide asymmetrically that will suggest mechanisms and guide studies in mammals, including humans. ..
  79. Gamma tubulin ring complex in microtubule nucleation
    Yixian Zheng; Fiscal Year: 2002
    ..abstract_text> ..
  80. Protein Interactions That Regulate Cell Polarity
    KENNETH PREHODA; Fiscal Year: 2005
    ..Finally, we will test the in vivo importance of these interactions in Drosophila epithelial and neuroblast cells, two models for cell polarity. ..
  81. Biochemical Basis of CENP-A Epigenetic Inheritance
    SAMANTHA ZEITLIN; Fiscal Year: 2005
    ..These studies will also have direct implications for understanding cancer causation, and applications in gene therapy. ..
  82. Regulation and Function of a Bacterial Cytoskeleton
    R Mullins; Fiscal Year: 2008
    ..3. Determine the effect of perturbing the biochemical properties of ParM and the ParR/parC complex on the accuracy and efficiency of plasmid segregation in vivo and in vitro. ..
  83. Nuclear Membrane Fusion in Xenopus Egg Extracts
    Martin Hetzer; Fiscal Year: 2007
    ..g. Rab GTPases) are involved in NE formation. As an alternative approach we will use photo-affinity methods, overlay assays and proteomic approaches to identify the second GTPase. ..
  84. DROSOPHILA & TUBULIN RING COMPLEX (ANDTURC)
    Yixian Zheng; Fiscal Year: 1999
    ....
  85. The Function of Dynein in Budding Yeast
    John Cooper; Fiscal Year: 2007
    ..We will focus on defining molecular mechanisms for these processes and understanding how they are regulated. ..
  86. Mitotic Motors in the Drosophila Embryo.
    Jonathan Scholey; Fiscal Year: 2001
    ..The results will contribute to an understanding of the basic mechanisms of mitosis and may provide insights into some of the dysfunctions in spindle action and chromosome segregation that characterize certain birth de ..
  87. HISTONE ACETYLTRANSFERASE AND TRANSCRIPTIONAL REGULATION
    Min Hao Kuo; Fiscal Year: 2001
    ..Moreover, individual roles played by the ADA and SAGA complexes in HIS3 expression are to be investigated both genetically and biochemically. ..
  88. Molecular Mechanisms in Actin Assembly & Cell Motility
    John Cooper; Fiscal Year: 2006
    ..This fundamental information may be the basis for the development of new therapies for human diseases involving the molecules and processes that are discovered and illuminated. ..
  89. Molecular Dissection of a Novel P53-like Protein
    Hua Lu; Fiscal Year: 2006
    ..Molecular dissection of the mechanisms underlying the regulation of p63 will shed light on our understanding of how this protein functions in cells. Moreover, this will have important implications for anti-tumor drug development...
  90. HMG CoA REDUCTASE AND COX2 INHIBITORS IN COLON CANCER
    CHINTHALAPALLY RAO; Fiscal Year: 2006
    ..Finally, we will study the effects of these agents on cell proliferation, and apoptosis during different stages of colon carcinogenesis. ..
  91. MECHANISMS FOR CHEMOPREVENTION OF COLON CANCER
    CHINTHALAPALLY RAO; Fiscal Year: 2001
    ..Tissue distribution and comparative metabolism studies of curcumin and PEMC will be studied with synthetic [3H] curcumin and [3H]-PEMC in vivo in male F-344 rats. ..
  92. PREVENTION OF COLORECTAL CANCER BY iNOS AND COX-2 SELECTIVE INHIBITORS
    CHINTHALAPALLY RAO; Fiscal Year: 2007
    ..abstract_text> ..