mitotic spindle apparatus

Summary

Summary: An organelle consisting of three components: (1) the astral microtubules, which form around each centrosome and extend to the periphery; (2) the polar microtubules which extend from one spindle pole to the equator; and (3) the kinetochore microtubules, which connect the centromeres of the various chromosomes to either centrosome.

Top Publications

  1. pmc The protein composition of mitotic chromosomes determined using multiclassifier combinatorial proteomics
    Shinya Ohta
    Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh EH9 3JR, UK
    Cell 142:810-21. 2010
  2. pmc A mechanism linking extra centrosomes to chromosomal instability
    Neil J Ganem
    Howard Hughes Medical Institute, Department of Pediatric Oncology, Dana Farber Cancer Institute, Children s Hospital, Boston, Massachusetts 02115, USA
    Nature 460:278-82. 2009
  3. ncbi The spindle-assembly checkpoint in space and time
    Andrea Musacchio
    Department of Experimental Oncology, European Institute of Oncology, Via Ripamonti 435, 20141 Milan, Italy
    Nat Rev Mol Cell Biol 8:379-93. 2007
  4. ncbi Mitotic spindle orientation in asymmetric and symmetric cell divisions during animal development
    Xavier Morin
    Institut de Biologie de l Ecole Normale Supérieure, 46 rue d Ulm, Paris, France
    Dev Cell 21:102-19. 2011
  5. ncbi Mitotic kinases as regulators of cell division and its checkpoints
    E A Nigg
    Max Planck Institute for Biochemistry, Department of Cell Biology, Am Klopferspitz 18a, D 82152 Martinsried, Germany
    Nat Rev Mol Cell Biol 2:21-32. 2001
  6. ncbi Mechanisms of asymmetric stem cell division
    Juergen A Knoblich
    Institute of Molecular Biotechnology of the Austrian Academy of Sciences, Dr Bohr Gasse 3, 1030 Vienna, Austria
    Cell 132:583-97. 2008
  7. pmc Chromosome- and spindle-pole-derived signals generate an intrinsic code for spindle position and orientation
    Tomomi Kiyomitsu
    Whitehead Institute, Nine Cambridge Center, Cambridge, Massachusetts 02142, USA
    Nat Cell Biol 14:311-7. 2012
  8. ncbi Control of apoptosis and mitotic spindle checkpoint by survivin
    F Li
    Boyer Center for Molecular Medicine, Department of Pathology, Yale University School of Medicine, New Haven, Connecticut 06536, USA
    Nature 396:580-4. 1998
  9. ncbi Cancer cells display profound intra- and interline variation following prolonged exposure to antimitotic drugs
    Karen E Gascoigne
    Faculty of Life Sciences, University of Manchester, Oxford Road, Manchester M13 9PT, UK
    Cancer Cell 14:111-22. 2008
  10. pmc Asymmetric cell divisions promote stratification and differentiation of mammalian skin
    Terry Lechler
    Howard Hughes Medical Institute, The Rockefeller University, New York, New York 10021, USA
    Nature 437:275-80. 2005

Detail Information

Publications280 found, 100 shown here

  1. pmc The protein composition of mitotic chromosomes determined using multiclassifier combinatorial proteomics
    Shinya Ohta
    Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh EH9 3JR, UK
    Cell 142:810-21. 2010
    ..Our integrated analysis predicts that up to 97 new centromere-associated proteins remain to be discovered in our data set...
  2. pmc A mechanism linking extra centrosomes to chromosomal instability
    Neil J Ganem
    Howard Hughes Medical Institute, Department of Pediatric Oncology, Dana Farber Cancer Institute, Children s Hospital, Boston, Massachusetts 02115, USA
    Nature 460:278-82. 2009
    ..These findings provide a direct mechanistic link between extra centrosomes and CIN, two common characteristics of solid tumours. We propose that this mechanism may be a common underlying cause of CIN in human cancer...
  3. ncbi The spindle-assembly checkpoint in space and time
    Andrea Musacchio
    Department of Experimental Oncology, European Institute of Oncology, Via Ripamonti 435, 20141 Milan, Italy
    Nat Rev Mol Cell Biol 8:379-93. 2007
    ..These studies are finally starting to reveal the mechanisms of checkpoint activation and silencing during mitotic progression...
  4. ncbi Mitotic spindle orientation in asymmetric and symmetric cell divisions during animal development
    Xavier Morin
    Institut de Biologie de l Ecole Normale Supérieure, 46 rue d Ulm, Paris, France
    Dev Cell 21:102-19. 2011
    ..Many recent studies show that future advances in the field of mitotic spindle orientation will arise from combinations of physical perturbation and modeling with classical genetics and developmental biology approaches...
  5. ncbi Mitotic kinases as regulators of cell division and its checkpoints
    E A Nigg
    Max Planck Institute for Biochemistry, Department of Cell Biology, Am Klopferspitz 18a, D 82152 Martinsried, Germany
    Nat Rev Mol Cell Biol 2:21-32. 2001
    ..Here, I give an overview of the many mitotic kinases that regulate cell division and the fidelity of chromosome transmission...
  6. ncbi Mechanisms of asymmetric stem cell division
    Juergen A Knoblich
    Institute of Molecular Biotechnology of the Austrian Academy of Sciences, Dr Bohr Gasse 3, 1030 Vienna, Austria
    Cell 132:583-97. 2008
    ..These distinct pathways have been elucidated mostly in Drosophila. Although the molecules involved are highly conserved in vertebrates, the way they act is tissue specific and sometimes very different from invertebrates...
  7. pmc Chromosome- and spindle-pole-derived signals generate an intrinsic code for spindle position and orientation
    Tomomi Kiyomitsu
    Whitehead Institute, Nine Cambridge Center, Cambridge, Massachusetts 02142, USA
    Nat Cell Biol 14:311-7. 2012
    ..We propose that these chromosome- and spindle-pole-derived gradients generate an intrinsic code to control spindle position and orientation...
  8. ncbi Control of apoptosis and mitotic spindle checkpoint by survivin
    F Li
    Boyer Center for Molecular Medicine, Department of Pathology, Yale University School of Medicine, New Haven, Connecticut 06536, USA
    Nature 396:580-4. 1998
    ..The overexpression of survivin in cancer may overcome this apoptotic checkpoint and favour aberrant progression of transformed cells through mitosis...
  9. ncbi Cancer cells display profound intra- and interline variation following prolonged exposure to antimitotic drugs
    Karen E Gascoigne
    Faculty of Life Sciences, University of Manchester, Oxford Road, Manchester M13 9PT, UK
    Cancer Cell 14:111-22. 2008
    ..We present data supporting a model wherein cell fate is dictated by two competing networks, one involving caspase activation, the other protecting cyclin B1 from degradation...
  10. pmc Asymmetric cell divisions promote stratification and differentiation of mammalian skin
    Terry Lechler
    Howard Hughes Medical Institute, The Rockefeller University, New York, New York 10021, USA
    Nature 437:275-80. 2005
    ..We further demonstrate that integrins and cadherins are essential for the apical localization of atypical protein kinase C, the Par3-LGN-Inscuteable complex and NuMA-dynactin to align the spindle...
  11. pmc Dissecting the role of MPS1 in chromosome biorientation and the spindle checkpoint through the small molecule inhibitor reversine
    Stefano Santaguida
    Department of Experimental Oncology, European Institute of Oncology, I 20139 Milan, Italy
    J Cell Biol 190:73-87. 2010
    ..They also shed light on the relationship between the error correction pathway and the spindle checkpoint and suggest that these processes are coregulated and are likely to share at least a subset of their catalytic machinery...
  12. pmc Systematic analysis of human protein complexes identifies chromosome segregation proteins
    James R A Hutchins
    Research Institute of Molecular Pathology IMP, Dr Bohr Gasse 7, A 1030 Vienna, Austria
    Science 328:593-9. 2010
    ..The approaches we describe here are generally applicable to high-throughput follow-up analyses of phenotypic screens in mammalian cells...
  13. ncbi Huntingtin is required for mitotic spindle orientation and mammalian neurogenesis
    Juliette D Godin
    Institut Curie, Orsay F 91405, France
    Neuron 67:392-406. 2010
    ..Moreover, Drosophila huntingtin restores spindle misorientation in mammalian cells. These findings reveal an unexpected role for huntingtin in dividing cells, with potential important implications in health and disease...
  14. pmc Histone H3 Thr-3 phosphorylation by Haspin positions Aurora B at centromeres in mitosis
    Fangwei Wang
    Division of Rheumatology, Immunology, and Allergy, Brigham and Women s Hospital, Harvard Medical School, Smith Building, 1 Jimmy Fund Way, Boston, MA 02115, USA
    Science 330:231-5. 2010
    ..Therefore, H3T3ph generated by Haspin positions the CPC at centromeres to regulate selected targets of Aurora B during mitosis...
  15. ncbi Aurora-A - a guardian of poles
    Tomotoshi Marumoto
    Department of Tumor Genetics and Biology, Graduate School of Medical Sciences, Kumamoto University, 1 1 1 Honjo, Kumamoto 860 8556, Japan
    Nat Rev Cancer 5:42-50. 2005
    ..What are the normal physiological roles of Aurora-A, how are these regulated and how might the enzyme function during tumorigenesis?..
  16. pmc Multipolar spindle pole coalescence is a major source of kinetochore mis-attachment and chromosome mis-segregation in cancer cells
    William T Silkworth
    Virginia Tech, Department of Biological Sciences, Blacksburg, VA, USA
    PLoS ONE 4:e6564. 2009
    ..We propose this spindle pole coalescence mechanism as a major contributor to chromosome instability in cancer cells...
  17. pmc Bicaudal D2, dynein, and kinesin-1 associate with nuclear pore complexes and regulate centrosome and nuclear positioning during mitotic entry
    Daniël Splinter
    Department of Cell Biology, Erasmus Medical Center, Rotterdam, The Netherlands
    PLoS Biol 8:e1000350. 2010
    ....
  18. pmc Asymmetric cell divisions promote Notch-dependent epidermal differentiation
    Scott E Williams
    Howard Hughes Medical Institute, Laboratory of Mammalian Cell Biology and Development, The Rockefeller University, New York, New York 10065, USA
    Nature 470:353-8. 2011
    ....
  19. ncbi Symmetry breaking in mouse oocytes requires transient F-actin meshwork destabilization
    Jessica Azoury
    UMR7622, CNRS UMPC, College de France, 9 quai Saint Bernard, Paris, France
    Development 138:2903-8. 2011
    ..Hence, the meshwork density drop at meiosis resumption is germane to the symmetry-breaking event required for successful asymmetric meiotic divisions...
  20. ncbi The bipolar mitotic kinesin Eg5 moves on both microtubules that it crosslinks
    Lukas C Kapitein
    Department of Physics and Astronomy and Laser Centre, Vrije Universiteit, De Boelelaan 1081, 1081 HV Amsterdam, The Netherlands
    Nature 435:114-8. 2005
    ..Our results demonstrate how members of the kinesin-5 family are likely to function in mitosis, pushing apart interpolar microtubules as well as recruiting microtubules into bundles that are subsequently polarized by relative sliding...
  21. pmc CDK5RAP2 is a pericentriolar protein that functions in centrosomal attachment of the gamma-tubulin ring complex
    Ka Wing Fong
    Department of Biochemistry, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
    Mol Biol Cell 19:115-25. 2008
    ..Our findings suggest that centrosome malfunction due to the CDK5RAP2 mutations may underlie autosomal recessive primary microcephaly...
  22. ncbi Microtubule-organizing centres: a re-evaluation
    Jens Lüders
    Department of Biological Sciences, Stanford University, CA 94305, USA
    Nat Rev Mol Cell Biol 8:161-7. 2007
    ..These findings suggest that the spatio-temporal control of microtubule nucleation is more complex than previously thought, leading us to a re-evaluation of the concept of the microtubule-organizing center...
  23. pmc Neuroepithelial stem cell proliferation requires LIS1 for precise spindle orientation and symmetric division
    Jessica Yingling
    Departments of Pediatrics and Medicine, Center for Human Genetics and Genomics, UCSD School of Medicine, 9500 Gilman Drive, La Jolla, CA 92098 0627, USA
    Cell 132:474-86. 2008
    ..Thus, control of symmetric division, essential for neuroepithelial stem cell proliferation, is mediated through spindle orientation determined via LIS1/NDEL1/dynein-mediated cortical microtubule capture...
  24. ncbi Proteome analysis of the human mitotic spindle
    Guido Sauer
    Max Planck Institute of Biochemistry, Department of Cell Biology, D 82152 Martinsried, Germany
    Mol Cell Proteomics 4:35-43. 2005
    ..This study illustrates the strength of a proteomic approach for the analysis of isolated human spindles and identifies several novel spindle components for future functional studies...
  25. pmc The spatial arrangement of chromosomes during prometaphase facilitates spindle assembly
    Valentin Magidson
    Wadsworth Center, New York State Department of Health, Albany, NY 12201 509, USA
    Cell 146:555-67. 2011
    ..Thus, spindle formation involves a previously overlooked stage of chromosome prepositioning which promotes formation of amphitelic attachments...
  26. ncbi Functionally unequal centrosomes drive spindle orientation in asymmetrically dividing Drosophila neural stem cells
    Elena Rebollo
    Cell Division Group, Institute for Research in Biomedicine, Parc Científic Barcelona, C Josep Samitier 1 5, 08028 Barcelona, Spain
    Dev Cell 12:467-74. 2007
    ..Apical aster maintenance requires the function of Pins. These results reveal that spindle orientation in Drosophila larval NBs is determined very early in the cell cycle, and is mediated by asymmetric centrosome function...
  27. ncbi Self-organization of MTOCs replaces centrosome function during acentrosomal spindle assembly in live mouse oocytes
    Melina Schuh
    Gene Expression Unit, European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    Cell 130:484-98. 2007
    ..Finally, a stable barrel-shaped acentrosomal metaphase spindle with oscillating chromosomes and astral-like microtubules forms that surprisingly exhibits key properties of a centrosomal spindle...
  28. pmc Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes
    Beate Neumann
    MitoCheck Project Group, European Molecular Biology Laboratory EMBL, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    Nature 464:721-7. 2010
    ..As part of the Mitocheck consortium, this study provides an in-depth analysis of cell division phenotypes and makes the entire high-content data set available as a resource to the community...
  29. ncbi Mechanisms of centrosome separation and bipolar spindle assembly
    Marvin E Tanenbaum
    Department of Medical Oncology, University Medical Center, CG Utrecht, The Netherlands
    Dev Cell 19:797-806. 2010
    ..Here, we discuss different pathways that promote initial centrosome separation and bipolar spindle assembly...
  30. ncbi Chromosome-induced microtubule assembly mediated by TPX2 is required for spindle formation in HeLa cells
    Oliver J Gruss
    EMBL, Meyerhofstrasse 1, 69 117 Heidelberg, Germany
    Nat Cell Biol 4:871-9. 2002
    ..This indicates that chromosome-induced microtubule production is a general requirement for the formation of functional spindles in animal cells...
  31. pmc The cortical protein Lte1 promotes mitotic exit by inhibiting the spindle position checkpoint kinase Kin4
    Daniela Trinca Bertazzi
    DKFZ ZMBH Alliance, German Cancer Research Center, 69120 Heidelberg, Germany
    J Cell Biol 193:1033-48. 2011
    ..This Lte1-mediated exclusion of Kin4 from the dSPB is essential for proper mitotic exit of cells with a correctly aligned spindle. Therefore, Lte1 promotes mitotic exit by inhibiting Kin4 activity at the dSPB...
  32. pmc Survivin reads phosphorylated histone H3 threonine 3 to activate the mitotic kinase Aurora B
    Alexander E Kelly
    Laboratory of Chromosome and Cell Biology, The Rockefeller University, New York, NY 10065, USA
    Science 330:235-9. 2010
    ..These findings establish a direct cellular role for mitotic histone H3T3 phosphorylation, which is read and translated by the CPC to ensure accurate cell division...
  33. pmc The roles of microtubule-based motor proteins in mitosis: comprehensive RNAi analysis in the Drosophila S2 cell line
    Gohta Goshima
    Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94107, USA
    J Cell Biol 162:1003-16. 2003
    ..From our phenotypic data, we construct a model for the distinct roles of molecular motors during mitosis in a single metazoan cell type...
  34. pmc Identification and characterization of two novel proteins affecting fission yeast gamma-tubulin complex function
    Srinivas Venkatram
    Department of Cell and Developmental Biology, Howard Hughes Medical Institute, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    Mol Biol Cell 15:2287-301. 2004
    ..This study expands the known roles of gamma-tubulin complex components in organizing different types of microtubule structures in S. pombe...
  35. pmc Integrin-mediated adhesion orients the spindle parallel to the substratum in an EB1- and myosin X-dependent manner
    Fumiko Toyoshima
    Department of Cell and Developmental Biology, Graduate School of Biostudies, Kyoto University, Sakyo ku, Kyoto, Japan
    EMBO J 26:1487-98. 2007
    ..Our results thus uncover the existence of a mechanism that orients the spindle parallel to the cell-substrate adhesion plane, and identify crucial factors involved in this novel mechanism...
  36. pmc The 3Ms of central spindle assembly: microtubules, motors and MAPs
    Michael Glotzer
    Department of Molecular Genetics and Cell Biology, University of Chicago, 920 East 58th Street, Chicago, Illinois 60637, USA
    Nat Rev Mol Cell Biol 10:9-20. 2009
    ..Complementary approaches, including cell biology, genetics and computational modelling, have provided new insights into the mechanism and regulation of central spindle assembly...
  37. ncbi External forces control mitotic spindle positioning
    Jenny Fink
    Institut Curie, CNRS UMR 144, 26 Rue d Ulm, 75248 Paris Cedex 05, France
    Nat Cell Biol 13:771-8. 2011
    ..We propose that cells divide according to cues provided by their mechanical micro-environment, aligning daughter cells with the external force field...
  38. pmc Pericentromeric sister chromatid cohesion promotes kinetochore biorientation
    Tessie M Ng
    Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
    Mol Biol Cell 20:3818-27. 2009
    ..Taken together, these data reveal a specific role for pericentromeric linkage in ensuring kinetochore biorientation...
  39. ncbi The cellular geography of aurora kinases
    Mar Carmena
    Wellcome Trust Centre for Cell Biology, Institute for Cell and Molecular Biology, Kings Buildings, University of Edinburgh, Mayfield Road, Edinburgh EH9 3JR, Scotland, UK
    Nat Rev Mol Cell Biol 4:842-54. 2003
  40. ncbi Spindle orientation bias in gut epithelial stem cell compartments is lost in precancerous tissue
    Aaron J Quyn
    Cell and Developmental Biology, University of Dundee, Dundee, DD1 5EH, UK
    Cell Stem Cell 6:175-81. 2010
    ..This loss correlates with cell shape changes specifically in the stem cell compartment. Our data suggest that loss of asymmetric division in stem cells might contribute to the oncogenic effect of Apc mutations in gut epithelium...
  41. pmc Making the Auroras glow: regulation of Aurora A and B kinase function by interacting proteins
    Mar Carmena
    Wellcome Trust Centre for Cell Biology, University of Edinburgh, Michael Swann Building, King s Buildings, Edinburgh, Scotland, UK
    Curr Opin Cell Biol 21:796-805. 2009
    ..This review will focus on how interacting proteins make this functional diversity possible by targeting the kinases to different subcellular locations and regulating their activity...
  42. ncbi Identification of a vertebrate sister-chromatid separation inhibitor involved in transformation and tumorigenesis
    H Zou
    Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Science 285:418-22. 1999
    ..The oncogenic nature of increased expression of vSecurin may result from chromosome gain or loss, produced by errors in chromatid separation...
  43. pmc A genome-wide RNAi screen to dissect centriole duplication and centrosome maturation in Drosophila
    Jeroen Dobbelaere
    The Gurdon Institute, Cambridge, United Kingdom
    PLoS Biol 6:e224. 2008
    ..Cnn is phosphorylated during mitosis in a Polo-dependent manner, suggesting that the Polo-dependent phosphorylation of Cnn initiates centrosome maturation in flies...
  44. ncbi The Ipl1-Aurora protein kinase activates the spindle checkpoint by creating unattached kinetochores
    Benjamin A Pinsky
    Fred Hutchinson Cancer Research Center, 1100 Fairview Ave N, PO Box 19024, Seattle, WA 98109, USA
    Nat Cell Biol 8:78-83. 2006
    ....
  45. pmc The CENP-S complex is essential for the stable assembly of outer kinetochore structure
    Miho Amano
    Department of Molecular Genetics, National Institute of Genetics, The Graduate University for Advanced Studies, Mishima, Shizuoka 411 8540, Japan
    J Cell Biol 186:173-82. 2009
    ..In addition, we found that intrakinetochore distance was increased in CENP-S- and CENP-X-deficient cells. These results suggest that the CENP-S complex is essential for the stable assembly of the outer kinetochore...
  46. ncbi A new model for asymmetric spindle positioning in mouse oocytes
    Melina Schuh
    European Molecular Biology Laboratory EMBL, Gene Expression Unit, Heidelberg, Germany
    Curr Biol 18:1986-92. 2008
    ..Based on these results, we propose the first mechanistic model for asymmetric spindle positioning in mammalian oocytes and validate five of its key predictions experimentally...
  47. pmc Mechanisms of microtubule-based kinetochore positioning in the yeast metaphase spindle
    Brian L Sprague
    Department of Biomedical Engineering, University of Minnesota, Minneapolis, Minnesota 55455, USA
    Biophys J 84:3529-46. 2003
    ..These results provide the first evidence of temporally stable spatial gradients of kMT catastrophe and/or rescue frequency in living cells...
  48. pmc Dynamic maintenance of asymmetric meiotic spindle position through Arp2/3-complex-driven cytoplasmic streaming in mouse oocytes
    Kexi Yi
    Stowers Institute for Medical Research, 1000 East 50th Street, Kansas City, Missouri 64110, USA
    Nat Cell Biol 13:1252-8. 2011
    ..Thus, the asymmetric MII spindle position is dynamically maintained as a result of balanced forces governed by the Arp2/3 complex...
  49. pmc KIF4 regulates midzone length during cytokinesis
    Chi Kuo Hu
    Department of Systems Biology, Harvard Medical School, Boston, MA 02115, USA
    Curr Biol 21:815-24. 2011
    ..Investigating midzone microtubule dynamics has been difficult in part because their plus ends are interdigitated and buried in a dense matrix, making them difficult to observe...
  50. ncbi Mammalian Pins is a conformational switch that links NuMA to heterotrimeric G proteins
    Quansheng Du
    Center for Cell Signaling, Department of Microbiology, University of Virginia School of Medicine, Charlottesville, VA 22908, USA
    Cell 119:503-16. 2004
    ..We propose that a related switch mechanism might operate in asymmetric cell divisions in the fly and nematode...
  51. pmc Evidence that Aurora B is implicated in spindle checkpoint signalling independently of error correction
    Stefano Santaguida
    Department of Experimental Oncology, European Institute of Oncology, Milan, Italy
    EMBO J 30:1508-19. 2011
    ..Our results support a model in which Aurora B is at the apex of a signalling pyramid whose sensory apparatus promotes the concomitant activation of error correction and checkpoint signalling pathways...
  52. pmc In vivo analysis of chromosome condensation in Saccharomyces cerevisiae
    Amit C J Vas
    Department of Genetics, Cell Biology, and Development, University of Minnesota Medical School, Minneapolis, MN 55455, USA
    Mol Biol Cell 18:557-68. 2007
    ..In vivo analysis of chromosome condensation has therefore revealed unsuspected relationships between higher order chromatin structure and cell cycle control...
  53. pmc The ch-TOG/XMAP215 protein is essential for spindle pole organization in human somatic cells
    Fanni Gergely
    Department of Pharmacology, University of Cambridge, Wellcome Trust Cancer Research UK Institute, Cambridge CB2 1QR, UK
    Genes Dev 17:336-41. 2003
    ..Thus, in human somatic cells, ch-TOG appears to play a major role in organizing spindle poles, and a more minor role in stabilizing spindle microtubules that is, at least in part, mediated via an interaction with TACC3...
  54. pmc Cep152 acts as a scaffold for recruitment of Plk4 and CPAP to the centrosome
    Onur Cizmecioglu
    Cell Cycle Control and Carcinogenesis, German Cancer Research Center DKFZ, 69120 Heidelberg, Germany
    J Cell Biol 191:731-9. 2010
    ..Our results suggest that Cep152 recruits Plk4 and CPAP to the centrosome to ensure a faithful centrosome duplication process...
  55. pmc Pharmacologic inhibition of the anaphase-promoting complex induces a spindle checkpoint-dependent mitotic arrest in the absence of spindle damage
    Xing Zeng
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Cancer Cell 18:382-95. 2010
    ..We propose that mutual antagonism between the APC and the SAC yields a positive feedback loop that amplifies the ability of TAME to induce mitotic arrest...
  56. pmc Mouse inscuteable induces apical-basal spindle orientation to facilitate intermediate progenitor generation in the developing neocortex
    Maria Pia Postiglione
    Institute of Molecular Biotechnology of the Austrian Academy of Sciences IMBA, Dr Bohr Gasse 3 5, 1030 Vienna, Austria
    Neuron 72:269-84. 2011
    ..Our results indicate that the orientation of progenitor cell divisions is important for correct lineage specification in the developing mammalian brain...
  57. pmc Cdc42 controls spindle orientation to position the apical surface during epithelial morphogenesis
    Aron B Jaffe
    Cell Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA
    J Cell Biol 183:625-33. 2008
    ..We conclude that Cdc42 regulates epithelial tissue morphogenesis by controlling spindle orientation during cell division...
  58. pmc Par3 controls epithelial spindle orientation by aPKC-mediated phosphorylation of apical Pins
    Yi Hao
    Department of Cell Biology, University of Virginia School of Medicine, Charlottesville, VA 22908, USA
    Curr Biol 20:1809-18. 2010
    ..Defects in signaling through Cdc42 and atypical protein kinase C (aPKC) also cause spindle misorientation. When epithelial cysts are grown in 3D cultures, misorientation creates multiple lumens...
  59. ncbi The role of Hklp2 in the stabilization and maintenance of spindle bipolarity
    David Vanneste
    Centre for Genomic Regulation, Universitat Pompeu Fabra, Barcelona, Spain
    Curr Biol 19:1712-7. 2009
    ..Our data indicate that its mechanism of action is clearly distinct from and complementary to that of Eg5, providing an additional understanding of the mechanism driving the formation and maintenance of the bipolar spindle...
  60. ncbi Genome-wide genetic analysis of polyploidy in yeast
    Zuzana Storchova
    Department of Pediatric Oncology, Dana Farber Cancer Institute, Boston, Massachusetts 02115, USA
    Nature 443:541-7. 2006
    ..Thus, geometric constraints may have profound effects on genome stability; the phenomenon described here may be relevant in a variety of biological contexts, including disease states such as cancer...
  61. ncbi The Aurora kinase family in cell division and cancer
    Gerben Vader
    Laboratory of Experimental Oncology, Department of Medical Oncology, University Medical Center Utrecht, Stratenum 2 125, Universiteitsweg 100, 3584 CG Utrecht, The Netherlands
    Biochim Biophys Acta 1786:60-72. 2008
    ....
  62. pmc Budding yeast chromosome structure and dynamics during mitosis
    C G Pearson
    Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    J Cell Biol 152:1255-66. 2001
    ..These results indicate that the elastic properties of DNA play an as of yet undiscovered role in the poleward movement of chromosome arms...
  63. pmc PRC1 is a microtubule binding and bundling protein essential to maintain the mitotic spindle midzone
    Cristiana Mollinari
    Institut de Biologie Structurale J P Ebel CEA CNRS, 38027 Grenoble Cedex 1, France
    J Cell Biol 157:1175-86. 2002
    ..We conclude that PRC1 is a microtubule-associated protein required to maintain the spindle midzone, and that distinct functions are associated with modular elements of the primary sequence...
  64. ncbi Cep97 and CP110 suppress a cilia assembly program
    Alexander Spektor
    Department of Pathology and NYU Cancer Institute, New York University School of Medicine, Smilow Research Center, 522 First Avenue, New York, NY 10016, USA
    Cell 130:678-90. 2007
    ..Identification of Cep97 and other genes involved in regulation of cilia assembly may accelerate our understanding of human ciliary diseases, including renal disease and retinal degeneration...
  65. pmc KIF14 and citron kinase act together to promote efficient cytokinesis
    Ulrike Gruneberg
    Department of Cell Biology, Max Planck Institute of Biochemistry, 82152 Martinsried, Germany
    J Cell Biol 172:363-72. 2006
    ..Atzori, E. Turco, R. Triolo, G.P. Dotto, et al. 2000. Neuron. 28:115-127), we find a general requirement for citron kinase in human cell division. Together, these findings identify a novel pathway required for efficient cytokinesis...
  66. ncbi The Mad1/Mad2 complex as a template for Mad2 activation in the spindle assembly checkpoint
    Anna De Antoni
    Department of Experimental Oncology, European Institute of Oncology, Via Ripamonti 435, 20141 Milano, Italy
    Curr Biol 15:214-25. 2005
    ..These events are linked to the existence of two distinct conformers of Mad2: a closed conformer bound to its kinetochore receptor Mad1 or its target in the checkpoint Cdc20 and an open conformer unbound to these ligands...
  67. pmc Functional analysis of human microtubule-based motor proteins, the kinesins and dyneins, in mitosis/cytokinesis using RNA interference
    Changjun Zhu
    The Burnham Institute, La Jolla, CA 92037, USA
    Mol Biol Cell 16:3187-99. 2005
    ..Using immunofluorescence analysis, time-lapse microscopy, and rescue experiments, we investigate the roles of these 12 kinesins in detail...
  68. ncbi Kif15 cooperates with eg5 to promote bipolar spindle assembly
    Marvin E Tanenbaum
    Department of Medical Oncology and Cancer Genomics Centre, University Medical Center Utrecht, The Netherlands
    Curr Biol 19:1703-11. 2009
    ..However, it is currently unclear whether and how additional motors can contribute to centrosome separation and bipolar spindle formation...
  69. ncbi Identification of genes required for cytoplasmic localization in early C. elegans embryos
    K J Kemphues
    Section of Genetics and Development Cornell University Ithaca, New York 14853
    Cell 52:311-20. 1988
    ..We propose that all of these defects result from the failure of a maternally encoded system for intracellular localization in early embryos...
  70. pmc Asymmetric CLASP-dependent nucleation of noncentrosomal microtubules at the trans-Golgi network
    Andrey Efimov
    Department of Cell and Developmental Biology, Vanderbilt University Medical Center, Nashville, TN 37232, USA
    Dev Cell 12:917-30. 2007
    ..We propose that Golgi-emanating microtubules contribute to the asymmetric microtubule networks in polarized cells and support diverse processes including post-Golgi transport to the cell front...
  71. pmc A lateral belt of cortical LGN and NuMA guides mitotic spindle movements and planar division in neuroepithelial cells
    Elise Peyre
    Universite de la Mediterranee Aix Marseille II, Developmental Biology Institute IBDML, Centre National de la Recherche Scientifique UMR6216, Case 907, Campus de Luminy, 13288 Marseille, France
    J Cell Biol 193:141-54. 2011
    ..The restricted localization of LGN and NuMA in the lateral belt is instructive for the planar alignment of the mitotic spindle, and required for its planar maintenance...
  72. pmc Pericentric chromatin is organized into an intramolecular loop in mitosis
    Elaine Yeh
    Department of Biology, 623 Fordham Hall CB 3280, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 3280, USA
    Curr Biol 18:81-90. 2008
    ..Cohesin is concentrated at the centromere region of the chromosome despite the fact that sister centromeres can be separated by 800 nm in vivo. The function of cohesin at sites of separated DNA is unknown...
  73. ncbi Control of mitotic spindle length
    Gohta Goshima
    Division of Biological Science, Graduate School of Science, Nagoya University, Nagoya 464 8602, Japan
    Annu Rev Cell Dev Biol 26:21-57. 2010
    ..Here we review molecules and mechanisms that control spindle length, how these mechanisms are deployed in different systems, and some quantitative models that describe the control of spindle length...
  74. pmc KNL1/Spc105 recruits PP1 to silence the spindle assembly checkpoint
    Jessica S Rosenberg
    Laboratory of Chromosome and Cell Biology, The Rockefeller University, New York, NY 10065, USA
    Curr Biol 21:942-7. 2011
    ..We propose that the PP1-Spc105 interaction enables local regulation of dynamic phosphorylation and dephosphorylation at the kinetochore to couple microtubule attachment and SAC silencing...
  75. pmc Human mutations in NDE1 cause extreme microcephaly with lissencephaly [corrected]
    Fowzan S Alkuraya
    Department of Genetics, King Faisal Specialist Hospital and Research Center, Riyadh 11211, Saudi Arabia
    Am J Hum Genet 88:536-47. 2011
    ....
  76. pmc Robust control of mitotic spindle orientation in the developing epidermis
    Nicholas D Poulson
    Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA
    J Cell Biol 191:915-22. 2010
    ..These data have uncovered two important regulatory points controlling ACD in the epidermis and allow a framework for analysis of how external cues control this important choice...
  77. ncbi A bouquet makes ends meet
    H Scherthan
    Department of Human Biology and Human Genetics, University of Kaiserslautern, D 67653 Kaiserslautern, Germany
    Nat Rev Mol Cell Biol 2:621-7. 2001
    ..There have been many hypotheses about its role in the pairing of meiotic chromosomes, but until recently these have been difficult to test...
  78. pmc Aurora kinases and protein phosphatase 1 mediate chromosome congression through regulation of CENP-E
    Yumi Kim
    Ludwig Institute for Cancer Research, University of California, San Diego, La Jolla, CA 92093, USA
    Cell 142:444-55. 2010
    ....
  79. pmc Ric-8A and Gi alpha recruit LGN, NuMA, and dynein to the cell cortex to help orient the mitotic spindle
    Geoffrey E Woodard
    B Cell Molecular Immunology Section, Laboratory of Immunoregulation, National Institute of Allergy and Infectious Disease, 10 Center Drive, MSC 1888, Bethesda, MD 20892 1752, USA
    Mol Cell Biol 30:3519-30. 2010
    ..These data indicate that Ric-8A signaling leads to assembly of a cortical signaling complex that functions to orient the mitotic spindle...
  80. ncbi Structure of a Survivin-Borealin-INCENP core complex reveals how chromosomal passengers travel together
    A Arockia Jeyaprakash
    Max Planck Institute of Biochemistry, Am Klopferspitz 18, D 82152 Martinsried, Germany
    Cell 131:271-85. 2007
    ..Association of the core "passenger" proteins creates a single structural unit, whose composite molecular surface presents conserved residues essential for central spindle and midbody localization...
  81. pmc LGN regulates mitotic spindle orientation during epithelial morphogenesis
    Zhen Zheng
    Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, GA 30912, USA
    J Cell Biol 189:275-88. 2010
    ..The normal apical exclusion of LGN during mitosis appears to be mediated by atypical PKC. Thus, cell polarization-mediated spatial restriction of spindle orientation determinants is critical for epithelial morphogenesis...
  82. ncbi CLASPs prevent irreversible multipolarity by ensuring spindle-pole resistance to traction forces during chromosome alignment
    Elsa Logarinho
    Instituto de Biologia Molecular e Celular, Universidade do Porto, Rua do Campo Alegre 823, 4150 180 Porto, Portugal
    Nat Cell Biol 14:295-303. 2012
    ..We propose that CLASPs and ninein confer spindle-pole resistance to traction forces exerted during chromosome congression, thereby preventing irreversible spindle multipolarity and aneuploidy...
  83. pmc Telomere-led bouquet formation facilitates homologous chromosome pairing and restricts ectopic interaction in fission yeast meiosis
    O Niwa
    Kazusa DNA Research Institute, 1532 3 Yana, Kisarazu, Chiba 292 0812, Japan
    EMBO J 19:3831-40. 2000
    ..Thus, telomere-led chromosome organization facilitates homologous pairing and also restricts irregular chromosome pairing during meiosis...
  84. ncbi Aurora B regulates formin mDia3 in achieving metaphase chromosome alignment
    Lina Cheng
    Department of Pathology and Cell Biology, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA
    Dev Cell 20:342-52. 2011
    ..These findings reveal a key role for mDia3 and its regulation by Aurora B phosphorylation in achieving proper stable kinetochore microtubule attachment...
  85. pmc Cdk5rap2 exposes the centrosomal root of microcephaly syndromes
    Timothy L Megraw
    Department of Biomedical Sciences, Florida State University, College of Medicine, Tallahassee, USA
    Trends Cell Biol 21:470-80. 2011
    ..We integrate the impact of centriole replication defects with the functions of Cdk5rap2 and other MCPH proteins, propose mechanisms for progenitor loss in MCPH, and discuss links to two other microcephaly syndromes...
  86. ncbi MAP4 and CLASP1 operate as a safety mechanism to maintain a stable spindle position in mitosis
    Catarina P Samora
    Centre for Mechanochemical Cell Biology, Warwick Medical School, University of Warwick, Coventry, CV4 7AL, UK
    Nat Cell Biol 13:1040-50. 2011
    ..These findings have wide biological importance, because spindle positioning is essential during embryogenesis and stem-cell homeostasis...
  87. ncbi Two-hybrid search for proteins that interact with Sad1 and Kms1, two membrane-bound components of the spindle pole body in fission yeast
    F Miki
    Kazusa DNA Research Institute, 292 0818, Kisarazu, Chiba, Japan
    Mol Genet Genomics 270:449-61. 2004
    ..We found that none of the newly identified genes tested were necessary for centromere/telomere clustering...
  88. pmc Constitutive Mad1 targeting to kinetochores uncouples checkpoint signalling from chromosome biorientation
    Maria Maldonado
    Laboratory of Chemistry and Cell Biology, The Rockefeller University, New York, New York 10065, USA
    Nat Cell Biol 13:475-82. 2011
    ....
  89. ncbi SIN and the art of splitting the fission yeast cell
    Andrea Krapp
    Cell Cycle Control Laboratory, Swiss Institute for Experimental Cancer Research ISREC, 1066 Epalinges, Switzerland
    Curr Biol 14:R722-30. 2004
    ..Flp1p, the S. pombe ortholog of Cdc14p, is not essential for mitotic exit, but may contribute to an orderly mitosis-G1 transition by regulating the destruction of the mitotic inducer cdc25p...
  90. pmc Protein phosphatase 6 regulates mitotic spindle formation by controlling the T-loop phosphorylation state of Aurora A bound to its activator TPX2
    Kang Zeng
    University of Liverpool, Cancer Research Centre, Liverpool L3 9TA, England, UK
    J Cell Biol 191:1315-32. 2010
    ..These results demonstrate a hitherto unappreciated role for PP6 as the T-loop phosphatase regulating Aurora A activity during spindle formation and suggest the general importance of this form of regulation...
  91. pmc Mitotic control of kinetochore-associated dynein and spindle orientation by human Spindly
    Ying Wai Chan
    Department of Cell Biology, Max Planck Institute of Biochemistry, D 82152 Martinsried, Germany
    J Cell Biol 185:859-74. 2009
    ..Collectively, our data reveal hSpindly-mediated dynein functions and highlight a critical role of KT dynein in spindle orientation...
  92. pmc Identification of an Aurora-A/PinsLINKER/Dlg spindle orientation pathway using induced cell polarity in S2 cells
    Christopher A Johnston
    Institute of Neuroscience, University of Oregon, Eugene, OR 97403, USA
    Cell 138:1150-63. 2009
    ..This "induced cortical polarity" assay is suitable for rapid identification of the proteins, domains, and amino acids regulating spindle orientation or cell polarity...
  93. pmc Aurora B couples chromosome alignment with anaphase by targeting BubR1, Mad2, and Cenp-E to kinetochores
    Claire Ditchfield
    School of Biological Sciences, University of Manchester, 2 205 Stopford Building, Oxford Rd, Manchester M13 9PT, UK
    J Cell Biol 161:267-80. 2003
    ..Together, these results suggest that by targeting checkpoint proteins to kinetochores, Aurora B couples chromosome alignment with anaphase onset...
  94. ncbi Validating Aurora B as an anti-cancer drug target
    Fiona Girdler
    Faculty of Life Sciences, Michael Smith Building, Oxford Road, University of Manchester, Manchester, M13 9PT, UK
    J Cell Sci 119:3664-75. 2006
    ..Because small molecule-mediated inhibition of Aurora A and Aurora B yields distinct phenotypes, our observations indicate that the Auroras may present two avenues for anti-cancer drug discovery...
  95. pmc The augmin complex plays a critical role in spindle microtubule generation for mitotic progression and cytokinesis in human cells
    Ryota Uehara
    Institute for Advanced Research, Nagoya University, Furo cho, Chikusa ku, Nagoya 464 8601, Japan
    Proc Natl Acad Sci U S A 106:6998-7003. 2009
    ..These results demonstrate that MT generation mediated by augmin and gamma-TuRC is critical for chromosome segregation and cytokinesis in human cells...
  96. pmc Human chromokinesin KIF4A functions in chromosome condensation and segregation
    Manjari Mazumdar
    National Cancer Institute, National Institutes of Health, Bldg 41, Rm B 507, 41 Library Dr, Bethesda, MD 20892, USA
    J Cell Biol 166:613-20. 2004
    ..Our results provide functional evidence that human KIF4A is a novel component of the chromosome condensation and segregation machinery functioning in multiple steps of mitotic division...
  97. pmc Exploring the functional interactions between Aurora B, INCENP, and survivin in mitosis
    Reiko Honda
    Department of Cell Biology, Max Planck Institute for Biochemistry, D 82152 Martinsried, Germany
    Mol Biol Cell 14:3325-41. 2003
    ..A nonphosphorylatable mutant (TSS893-895AAA) was a poor activator of Aurora B, demonstrating that INCENP phosphorylation is important for kinase activation...
  98. pmc Arp2/3 complex regulates asymmetric division and cytokinesis in mouse oocytes
    Shao Chen Sun
    Department of Animal Sciences, Chungbuk National University, Cheongju, Republic of Korea
    PLoS ONE 6:e18392. 2011
    ..Our data suggest that the Arp2/3 complex probably regulates oocyte polarization through its effect on spindle migration, asymmetric division and cytokinesis during mouse oocyte meiotic maturation...
  99. ncbi CIK1: a developmentally regulated spindle pole body-associated protein important for microtubule functions in Saccharomyces cerevisiae
    B D Page
    Department of Biology, Yale University, New Haven, Connecticut 06511
    Genes Dev 6:1414-29. 1992
    ....
  100. ncbi Targeting of the RhoGEF Ect2 to the equatorial membrane controls cleavage furrow formation during cytokinesis
    Kuan Chung Su
    Cell Division and Aneuploidy Laboratory, Cancer Research UK London Research Institute, Clare Hall Laboratories, Blanche Lane, South Mimms, Hertfordshire EN6 3LD, UK
    Dev Cell 21:1104-15. 2011
    ..We propose that targeting of Ect2 to the equatorial membrane represents a key step in the delivery of the cytokinetic signal to the cortex...
  101. ncbi Nuclear transport and the mitotic apparatus: an evolving relationship
    Richard Wozniak
    Department of Cell Biology, University of Alberta, Edmonton, AB, T6G 2H7, Canada
    Cell Mol Life Sci 67:2215-30. 2010
    ..In this review, we will discuss this evolving area of study and emerging hypotheses that propose key roles for components of the nuclear transport apparatus in mitotic progression...

Research Grants65

  1. Tubulin-Binding Upconversion Nanoparticles for Breast-Cancer Imaging and Therapy
    Chuanbin Mao; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Normal cell division needs the formation of a mitotic spindle apparatus, which is directly related to microtubule (MT) assembly...
  2. Role of Mitotic Checkpoint Defects in Mouse Tumor Models
    ROBERT I BENEZRA; Fiscal Year: 2010
    ..a cell prior to the metaphase to anaphase transition if all the chromosomes are not attached to the mitotic spindle apparatus. Partial loss of function mutations predisposes mammalian cells to chromosome missegregation events and ..
  3. YEAST CHROMOSOME STRUCTURE, REPLICATION AND SEGREGATION
    Victoria Lundblad; Fiscal Year: 2000
    ..telomeres and position effects, centromeres and their interacting proteins, components of the mitotic spindle apparatus, microtubule motors, meiotic chromosome segregation and chromosome cohesion...
  4. SIGNALING PATHWAYS THAT REGULATE MITOSIS
    THOMAS GUADAGNO; Fiscal Year: 2005
    ..2. Determine the role MAPK plays in regulating the formation and function of the mitotic spindle apparatus. 3. Determine the biochemical steps that regulate MAPK activation during mitosis...
  5. MOLECULAR AND DEVELOPMENTAL GENETICS OF DROSOPHILA
    William Gelbart; Fiscal Year: 1993
    ..e) Study the in vitro properties and in vivo function of the products of two genes, ned and nod, which belong to the kinesin superfamily and movements of microtubules in the meiotic and mitotic spindle apparatus.
  6. MITOTIC CHECKPOINT GENE MAD2 IN VERTEBRATES
    Robert Benezra; Fiscal Year: 2004
    ..a cell prior to the metaphase to anaphase transition if all the chromosomes are not attached to the mitotic spindle apparatus. The main goal of the research being proposed is to determine if complete or partial loss of Mad2 ..
  7. GENETIC CONTROL OF MITOTIC SPINDLE ASSEMBLY AND FUNCTION
    Daniel Burke; Fiscal Year: 2005
    ..conserved mitotic regulatory system that arrests cells when chromosomes are not properly aligned on the mitotic spindle apparatus. Mutations in spindle checkpoint genes have been implicated in genomic instability and tumor progression ..
  8. DELTAVISION RESTORATION MICROSCOPY SYSTEM MODEL 483
    Conly Rieder; Fiscal Year: 2001
    ..This Core will maintain the instrument and provide technical support to those Principal Investigators/Program Directors who are in need of low-light high- resolution 3-D fluorescent imaging. ..
  9. Cytokinesis and the Cytoskeleton in C. elegans Embryos
    BRUCE A BOWERMAN; Fiscal Year: 2010
    ..Matthias Peter at the ETH in Zurich, Switzerland. The processes and genes we propose to investigate are relevant to important human diseases, including cancer and neurodegenerative disease. ..
  10. Gene networks specifying cell lineages in a polychaete
    Bruce Bowerman; Fiscal Year: 2007
    ..Newly discovered and conserved loci will also be studied in C. elegans and Drosophila. [unreadable] [unreadable]..
  11. JEOL JEM-1010 transmission electron microscope & Camera
    Duane Compton; Fiscal Year: 2002
    ..The research of the principle investigators cannot be completed without continued availability of a transmission electron microscope. ..
  12. Metotic Spindle Assembly and Aneuploidy in Mammals
    Duane Compton; Fiscal Year: 2003
    ..abstract_text> ..
  13. Misregulation of apoptosis in cloned pig embryos
    Heide Schatten; Fiscal Year: 2007
    ..Pilot data from this R03 small grants program research will be used to apply for funding through the R01 mechanism. [unreadable] [unreadable] [unreadable]..
  14. MARCKS Phosphorylation in Growth Cone Signaling
    JESSE GATLIN; Fiscal Year: 2004
    ..Functional consequences of aberrant PKC signaling will be determined by observing the turning response of growth cones from DRG neurons expressing a dominant-negative, phosphorylation-deficient MARCKS mutant. ..
  15. Structure and Function of a Eukaryotic Centromere
    Kerry S Bloom; Fiscal Year: 2010
    ....
  16. Intraflagellar Transport Motors in C. elegans neurons
    Jonathan M Scholey; Fiscal Year: 2010
    ....
  17. Cytoskeletal organization in apicomplexan parasites
    Heide Schatten; Fiscal Year: 2005
    ..Pilot data from this RO3 research will be used to apply for funding through the RO1 mechanism. ..
  18. Intrinsic Spindle Mechanisms to Insure Bipolarity
    JESSE GATLIN; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  19. Mitochondria distribution in cloned pig embryos
    Heide Schatten; Fiscal Year: 2005
    ..Pilot data from this RO3 small grants program research will be used to apply for funding through the RO1 mechanism. ..
  20. Sixth International Conference on Systems Biology (ICSB 2005)
    Andrew Murray; Fiscal Year: 2005
    ..The speakers and contributors will include experimental biologists, chemists, computer scientists, physicists, engineers, and mathematicians. ..
  21. Molecular Dissection of Cytokinesis
    Michael Glotzer; Fiscal Year: 2009
    ..Furthermore, molecular dissection of ZEN-4 will enhance our understanding of the mechanism of action of microtubule motors. ..
  22. Cloning and analysis of centrosome-associated RNA
    MARK ALLIEGRO; Fiscal Year: 2007
    ..If our hypothesis is correct, this study will reopen a line of inquiry with impact in areas of cell and developmental biology ranging from control of cell division to evolution of eukaryotes. [unreadable] [unreadable]..
  23. Novel Genes Expressed in Proliferating Endothelial Cells
    MARK ALLIEGRO; Fiscal Year: 2006
    ..abstract_text> ..
  24. Probing Cell Division with Synthetic Chemistry
    Timothy Mitchison; Fiscal Year: 2008
    ..Having found small molecule tools that perturb cell division by novel mechanisms, we will test their ability to kill cancer cells in vitro. ..
  25. Acquisition of a High-throughput Confocal Imaging System
    Claire Walczak; Fiscal Year: 2008
    ..Overall the proposed instrumentation would have a significant impact on the research approaches and progress made by life sciences researchers at our university. [unreadable] [unreadable] [unreadable]..
  26. FLUORESCENT SPECKLE MICROSCOPY
    EDWARD SALMON; Fiscal Year: 2007
    ..Experience gained in the course of these studies will be used to direct and refine hardware and software development. [unreadable] [unreadable]..
  27. Cytoskeleton polymerization dynamics in the cell cycle
    Timothy J Mitchison; Fiscal Year: 2010
    ..Understanding midbody assembles will reveal principles for generating asymmetric assemblies in the cytoplasm, and will tell us how the cleavage furrow is positioned. ..
  28. Regulation and function of the Clp 1p protein phosphatase
    Dannel McCollum; Fiscal Year: 2010
    ..3) To test a hypothesis that Clp1 promotes actomyosin ring stability through effects on Cdc15. 4) To determine how Clp1 and the anaphase promoting complex promote Cdc25 inactivation in late mitosis. ..
  29. Mechanisms of Microtubule Dynamic Turnover
    Patricia Wadsworth; Fiscal Year: 2009
    ..Learning how normal cells construct a mitotic spindle is an important first step towards understanding defective cell division in cancer. ..
  30. Purchase of Leica TCS SP2 Confocal Microscope
    Patricia Wadsworth; Fiscal Year: 2002
    ....
  31. MECHANISM OF ACTION OF CRYTOPHYCINS
    Susan Mooberry; Fiscal Year: 2002
    ..The differences in the biological responses of the congeners will determine the relationships between biological events and the ultimate ability to initiate apoptosis. ..
  32. MECHANISMS OF MITOTIC SPINDLE ASSEMBLY AND FUNCTION
    EDWARD SALMON; Fiscal Year: 2003
    ..Finally, we will analyze how MT dynamics may be involved in the regulation of cytokinesis. ..
  33. SPINDLE POLES AND NUCLEAR PORES--YEAST NDC1 AND MPS2
    Mark Winey; Fiscal Year: 2003
    ..The proposed project is aimed at understanding the function of a set of unusual membrane proteins at the SPB and the NPC, and at identifying other proteins with which the interact at these organelles. ..
  34. Molecular Dissection of the "Pacman-Flux" Machinery Used to Move Chromosomes
    DAVID JAMES SHARP; Fiscal Year: 2010
    ..An understanding of how this process occurs normally should provide insights into the molecular etiology of these diseases and suggest therapeutic strategies for their treatment. ..
  35. ELECTRON MICROSCOPE
    Timothy Mitchison; Fiscal Year: 2004
    ..The automated controls and digital image acquisition will also be optimal for introducing new users to electron microscopy and for minimizing maintenance requirements that might arise from operator inexperience. ..
  36. SISTER CHROMATID LINKAGE AND SEPARATION
    Andrew Murray; Fiscal Year: 2004
    ..This information will be directly relevant to understanding how aneuploidy is generated in Down syndrome and tumor progression. ..
  37. Tetrahymena Basal Body Duplication
    Mark Winey; Fiscal Year: 2004
    ....
  38. SPINDLE ASSEMBLY CHECKPOINT IN VERTEBRATES AND YEAST
    Rey Huei Chen; Fiscal Year: 2003
    ..The knowledge will then be applied to the question of how the deregulation of the checkpoint function may contribute to cancer progression. ..
  39. ACTIN DYNAMICS AND CELL LOCOMOTION
    Timothy Mitchison; Fiscal Year: 2004
    ..For perturbation experiments we will use protein tools, and also cell permeable chemicals coming out of a screening effort at the Harvard Institute of Chemistry and Cell Biology (ICCB). ..
  40. Role of Nuf2 in Kinetochore Function
    Jennifer DeLuca; Fiscal Year: 2004
    ..abstract_text> ..
  41. Genomic instability induced by the HPV-16 E7 oncoprotein
    Stefan Duensing; Fiscal Year: 2009
    ....
  42. The Function of Dynein in Budding Yeast
    John Cooper; Fiscal Year: 2009
    ..We will focus on defining molecular mechanisms for these processes and understanding how they are regulated. ..
  43. RED CELL CYTOSKELETAL PROTEIN AND MITOSIS
    Shu Ching Huang; Fiscal Year: 2008
    ..Finally, we shall define the relationship between changes in the expression of these 4.1R isoforms and the transition from proliferation to post-mitotic states during erythroid differentiation. ..
  44. Septins and the Cell Cycle in Budding Yeast
    John Cooper; Fiscal Year: 2009
    ..To test this hypothesis, we will examine septin filament architecture with respect to the cell cycle and checkpoint activation. ..
  45. Regulation of the Anaphase-Promoting Complex by the Spindle Checkpoint
    Hongtao Yu; Fiscal Year: 2010
    ..Experiments in this proposal will shed light on the molecular basis of the spindle checkpoint, which might eventually lead to new strategies to treat human cancers. ..
  46. Mechanisms of Action of Anti-Mitotic Drugs
    Mary Jordan; Fiscal Year: 2007
    ..The results will elucidate mechanisms and predict possible clinical efficacy of combination therapies directed at modulating mitotic microtubule regulatory proteins in combination with taxanes and Vinca alkaloids. ..
  47. Interdisciplinary Training in Quantitative Cell Biology
    RONALD VALE; Fiscal Year: 2006
    ..In this application we request support for this exciting endeavor. ..
  48. Macromoleclar Complexes Involved in Cellular Transport
    RONALD VALE; Fiscal Year: 2005
    ..These studies will provide a mechanistic understanding of how cargoes bind to specific motors and how cells asymmetrically position components within their cytoplasm. ..
  49. MECHANISMS OF MICROTUBULE AND MITOTIC SPINDLE ASSEMBLY
    Lynne Cassimeris; Fiscal Year: 2006
    ..To address these issues, we will apply a number of fluorescence-based imaging methods combined with siRNA or injection of function-blocking proteins or antibodies. ..
  50. NEGATIVE REGULATION OF CELL CYCLE PROGRESSION
    M Hoyt; Fiscal Year: 2004
    ..The identified genes will report checkpoint activation and will allow a powerful dissection of this signal transduction pathway. ..
  51. Olympus FV1000 Laser Scanning Confocal Microscope
    William Bement; Fiscal Year: 2007
    ..This information, in turn, will help guide future investigations of a number of normal and pathological human processes such as wound repair, nerve regeneration, cancer, and birth defects. [unreadable] [unreadable] [unreadable]..
  52. SHARED HIGH RESOLUTION MULTIMODE MICROSCOPE SYSTEM
    Greenfield Sluder; Fiscal Year: 2001
    ..The proposed instrument system will allow these researchers to take their investigations in new directions, obtain novel information, and thus, significantly advance their programs in basic biomedical research. ..
  53. DELTAVISION MICROSCOPE SYSTEM
    Linda Wordeman; Fiscal Year: 2001
    ..The operation and management of the equipment, the internal advisory committee, and institutional support for Deltavision are described. ..
  54. Asymmetric cell division in the C elegans embryo
    Robert Goldstein; Fiscal Year: 2007
    ..We expect that we will learn important new information about how cells divide asymmetrically that will suggest mechanisms and guide studies in mammals, including humans. ..
  55. Mechanism of Poly-ADP-ribose function in the spindle
    Paul Chang; Fiscal Year: 2005
    ..Preliminary experiments demonstrate a requirement for PAR in spindle function. Here, I propose to examine the mechanism by which PAR acts in the mitotic spindle. ..
  56. Protection of Centromeric Cohesion by Bub1 and Sgo1
    Hongtao Yu; Fiscal Year: 2010
    ....
  57. Epigenetic Control of Heterochromatin Formation
    Rabindranath De La Fuente; Fiscal Year: 2010
    ..abstract_text> ..
  58. Cytochrome C Oxidase I:A Biomarker for Colon Cancer Risk
    Claire Payne; Fiscal Year: 2006
    ..Various molecular and cellular techniques will be used in these studies. ..
  59. G2 Role For Cdk2 in Human Cells
    Gregory Enders; Fiscal Year: 2007
    ..We believe that deregulation of Cdk2, a frequent event in cancer, may contribute to genetic instability and neoplastic progression through deregulation of these functions. ..
  60. A DECONVOLUTION MICROSCOPE FOR CELL BIOLOGICAL RESEARCH
    Mark Rose; Fiscal Year: 2001
    ..By these means, the digital deconvolution microscope will provide outstanding research and educational opportunities on one of the most powerful optical microscopes available for use in the life sciences. ..
  61. MODULATION OF CERAMIDE INDUCED APOPTOSIS IN VIVO
    Richard Kolesnick; Fiscal Year: 2009
    ..If results indicate that overexpressing ASMase effectively radiosensitizes tumor microvasculature, applicability to human tumor radiotherapy will be examined directly using human versions of lentiviral asmase vectors. ..
  62. G-protein signal coordination by RGS12
    DAVID SIDEROVSKI; Fiscal Year: 2009
    ..We wish to study one RGS protein, RGS12, that interferes with the actions of the neurotransmitter GABA in neurons that control pain processing. This work should lead to the discovery of new pain-controlling drugs. ..
  63. Biochemical Basis of CENP-A Epigenetic Inheritance
    SAMANTHA ZEITLIN; Fiscal Year: 2005
    ..These studies will also have direct implications for understanding cancer causation, and applications in gene therapy. ..
  64. Molecular Dissection of a Novel P53-like Protein
    Hua Lu; Fiscal Year: 2006
    ..Molecular dissection of the mechanisms underlying the regulation of p63 will shed light on our understanding of how this protein functions in cells. Moreover, this will have important implications for anti-tumor drug development...