Genomes and Genes
Summary: The residual framework structure of the CELL NUCLEUS that maintains many of the overall architectural features of the cell nucleus including the nuclear lamina with NUCLEAR PORE complex structures, residual CELL NUCLEOLI and an extensive fibrogranular structure in the nuclear interior. (Advan. Enzyme Regul. 2002; 42:39-52)
Publications315 found, 100 shown here
- HA95 is a protein of the chromatin and nuclear matrix regulating nuclear envelope dynamicsS B Martins
Institute of Medical Biochemistry, University of Oslo, PO Box 1112 Blindern, Norway
J Cell Sci 113:3703-13. 2000..Biochemical and photobleaching data indicate that HA95 is tightly associated with chromatin and the nuclear matrix/lamina network in interphase, and bound to chromatin at mitosis...
- Internal organisation of the nucleus: assembly of compartments by macromolecular crowding and the nuclear matrix modelRonald Hancock
Laval University Cancer Research Centre, Hotel Dieu Hospital, 9 rue MacMahon, Quebec, P Q G1R 2J6, Canada
Biol Cell 96:595-601. 2004..nucleus are segregated into discrete compartments, but the current model that this is achieved by a fibrillar nuclear matrix which structures the nuclear interior and compartments is not consistent with all experimental observations, ..
- Nuclear matrix support of DNA replicationBoyka Anachkova
Institute of Molecular Biology, Bulgarian Academy of Sciences, Akad G Bonchev Street, Bl 21, Sofia 1113, Bulgaria
J Cell Biochem 96:951-61. 2005..To achieve this, DNA is organized into loops attached to the nuclear matrix. Each loop represents one individual replicon with the origin of replication localized within the loop and the ..
- PIASy, a nuclear matrix-associated SUMO E3 ligase, represses LEF1 activity by sequestration into nuclear bodiesS Sachdev
Gene Center and Institute of Biochemistry, University of Munich, 81377 Munich, Germany
Genes Dev 15:3088-103. 2001..Moreover, PIASy binds to nuclear matrix-associated DNA sequences and targets LEF1 to nuclear bodies, suggesting that PIASy-mediated subnuclear ..
- The sperm nuclear matrix is required for paternal DNA replicationJeffrey A Shaman
Institute for Biogenesis Research, John A Burns School of Medicine, University of Hawaii, Honolulu, Hawaii 96822, USA
J Cell Biochem 102:680-8. 2007..We previously demonstrated that mammalian sperm nuclei contain a nuclear matrix that organizes the DNA into loop domains in a manner similar to that of somatic cells...
- Human telomeres are attached to the nuclear matrixT de Lange
Rockefeller University, New York, NY 10021
EMBO J 11:717-24. 1992This report shows that human telomeres are tightly associated with the nuclear matrix. Telomere attachment is observed in several cell types and in all stages of interphase...
- An intact sperm nuclear matrix may be necessary for the mouse paternal genome to participate in embryonic developmentW S Ward
Division of Urology, Robert Wood Johnson Medical School, New Brunswick, New Jersey 08903, USA
Biol Reprod 60:702-6. 1999..We also tested the stability of the sperm nuclear matrix by the ability to form nuclear halos. Sperm nuclei washed in freshly prepared 0...
- A novel DNA-binding motif in the nuclear matrix attachment DNA-binding protein SATB1K Nakagomi
La Jolla Cancer Research Foundation, California 92037
Mol Cell Biol 14:1852-60. 1994The nuclear matrix attachment DNA (MAR) binding protein SATB1 is a sequence context-specific binding protein that binds in the minor groove, making virtually no contact with the DNA bases...
- A hyperphosphorylated form of the large subunit of RNA polymerase II is associated with splicing complexes and the nuclear matrixM J Mortillaro
Department of Biological Sciences, State University of New York at Buffalo 14260, USA
Proc Natl Acad Sci U S A 93:8253-7. 1996..retained in a similar pattern following in situ extraction of cells and was quantitatively recovered in the nuclear matrix fraction...
- The role of primer recognition proteins in DNA replication: association with nuclear matrix in HeLa cellsJ K Vishwanatha
Department of Biochemistry, University of Nebraska Medical Center, Omaha 68198 4525
J Cell Sci 101:25-34. 1992..we have investigated the subcellular distribution and specific association of these proteins with the nuclear matrix in HeLa cells...
- A proteomic study of the arabidopsis nuclear matrixTomasz T Calikowski
Department of Plant Biology and Plant Biotechnology Center, Ohio State University, Columbus, Ohio 43210, USA
J Cell Biochem 90:361-78. 2003..to be organized by two interdependent nucleoprotein structures, the DNA-based chromatin and the RNA-dependent nuclear matrix. The functional composition and molecular organization of the second component have not yet been resolved...
- Functional domains of necdin for protein-protein interaction, nuclear matrix targeting, and cell growth suppressionHideo Taniura
Graduate School of Natural Science and Technology, Kanazawa University, Kakumamachi, Kanazawa, Ishikawa 920 1192, Japan
J Cell Biochem 94:804-15. 2005..We here characterize the regions of necdin required for the protein-protein interaction, nuclear matrix targeting, and cell growth suppression...
- Dynamics of nuclear matrix proteome during embryonic development in Drosophila melanogasterParul Varma
Center for Cellular and Molecular Biology CSIR, Hyderabad 500 007, India
J Biosci 36:439-59. 2011..of nuclear architecture, which is believed to play an important role in regulation of gene expression, is the nuclear matrix (NuMat)...
- Lamin proteins form an internal nucleoskeleton as well as a peripheral lamina in human cellsP Hozak
CRC Nuclear Structure and Function Research Group, Sir William Dunn School of Pathology, University of Oxford, UK
J Cell Sci 108:635-44. 1995..Knobs and nodes on an internal skeleton can then be immunolabelled using fluorescein- or gold-conjugated anti-lamin A antibodies. These results suggest that the lamins are misnamed as they are also found internally...
- Necdin interacts with the ribonucleoprotein hnRNP U in the nuclear matrixHideo Taniura
Division of Regulation of Macromolecular Functions, Institute for Protein Research, Osaka University, Yamadaoka 3 2, Suita, Osaka 565 0871, Japan
J Cell Biochem 84:545-55. 2002..contained cDNA encoding a carboxyl-terminal portion of heterogeneous nuclear ribonucleoprotein U (hnRNP U), a nuclear matrix-associated protein that interacts with chromosomal DNA...
- A novel form of the telomere-associated protein TIN2 localizes to the nuclear matrixPatrick G Kaminker
Buck Institute for Age Research, Novato, California, USA
Cell Cycle 8:931-9. 2009..TIN2S) in two ways: TIN2L contains an additional 97 amino acids, and TIN2L associates strongly with the nuclear matrix. Stringent salt and detergent conditions failed to extract TIN2L from the nuclear matrix, despite removing ..
- PDLIM2 suppresses human T-cell leukemia virus type I Tax-mediated tumorigenesis by targeting Tax into the nuclear matrix for proteasomal degradationPengrong Yan
University of Pittsburgh Cancer Institute, University of Pittsburgh Medical Center, PA, USA
Blood 113:4370-80. 2009..In addition, PDLIM2 recruited Tax from its functional sites into the nuclear matrix where the polyubiquitinated Tax was degraded by the proteasome...
- From DNA structure to gene expression: mediators of nuclear compartmentalization and dynamicsJ Bode
GBF German Research Center for Biotechnology Epigenetic Regulation, Mascheroder Weg 1, D 38124 Braunschweig
Chromosome Res 11:435-45. 2003..into chromatin domains by their attachment to a supporting structure that has traditionally been termed the nuclear matrix. Present evidence indicates the dynamics of this entity, which requires particular properties of the elements ..
- CENP-F is a protein of the nuclear matrix that assembles onto kinetochores at late G2 and is rapidly degraded after mitosisH Liao
Fox Chase Cancer Center, Institute for Cancer Research, Philadelphia, Pennsylvania 19111, USA
J Cell Biol 130:507-18. 1995..CENP-F is protein of the nuclear matrix that gradually accumulates during the cell cycle until it reaches peak levels in G2 and M phase cells and is ..
- Anchoring the genomeDiego Ottaviani
Cancer Research UK London Research Institute, Lincoln s Inn Fields, London WC2A 3PX, UK
Genome Biol 9:201. 2008..is evidence that higher-order chromatin folding is mediated by the anchoring of specific DNA sequences to the nuclear matrix. These genome anchors are also crucial regulators of gene expression and DNA replication, and play a role in ..
- Dynamics of association of origins of DNA replication with the nuclear matrix during the cell cycleV Djeliova
Institute of Molecular Biology, Bulgarian Academy of Sciences, Akad. G. Bonchev Street, Bl. 21, Sofia 1113, Bulgaria
Nucleic Acids Res 29:3181-7. 2001DNA of replication foci attached to the nuclear matrix was isolated from Chinese hamster ovary cells and human HeLa cells synchronized at different stages of the G(1) and S phases of the cell cycle...
- Fanconi anemia proteins localize to chromatin and the nuclear matrix in a DNA damage- and cell cycle-regulated mannerF Qiao
Departments of Microbiology and Pediatrics, University of Virginia and the University of Virginia Health System, Charlottesville, Virginia 22908, USA
J Biol Chem 276:23391-6. 2001..Biochemical fractionation reveals that the FA proteins are found in nuclear matrix and chromatin and that treatment with mitomycin C results in increase of the FA proteins in nuclear matrix and ..
- Relationship between DNA replication and the nuclear matrixRosemary H C Wilson
Department of Biology, University of York, Heslington, York, YO10 5DD, UK
Genes Cells 18:17-31. 2013There is an extensive list of primary published work related to the nuclear matrix (NM)...
- The fate of the nuclear matrix-associated-region-binding protein SATB1 during apoptosisJ Gotzmann
Institute of Tumor Biology Cancer Research, University of Vienna, A 1090 Vienna, Austria
Cell Death Differ 7:425-38. 2000..protein 1 (SATB1), predominantly expressed in thymocytes, was identified as a component of the nuclear matrix protein fraction...
- Minicircle performance depending on S/MAR-nuclear matrix interactionsSandra Broll
Department Molecular Biotechnology Epigenetic Regulation, Helmholtz Center for Infection Research, Inhoffenstrasse 7, D 38124 Braunschweig, Germany
J Mol Biol 395:950-65. 2010....
- Identification of a candidate regulatory region in the human CD8 gene complex by colocalization of DNase I hypersensitive sites and matrix attachment regions which bind SATB1 and GATA-3Lynda J Kieffer
Department of Laboratory Medicine and Department of Genetics and Section of Immunobiology, Yale University, New Haven, CT 06520, USA
J Immunol 168:3915-22. 2002To locate elements regulating the human CD8 gene complex, we mapped nuclear matrix attachment regions (MARs) and DNase I hypersensitive (HS) sites over a 100-kb region that included the CD8B gene, the intergenic region, and the CD8A gene...
- Nuclear matrix bound fibroblast growth factor receptor is associated with splicing factor rich and transcriptionally active nuclear specklesSuryanarayan Somanathan
Department of Biological Sciences, State University of New York at Buffalo, Buffalo, New York 14260, USA
J Cell Biochem 90:856-69. 2003..arrangements of FGFR1 sites and colocalization with nuclear speckles were maintained following extraction for nuclear matrix. Moreover, immunoblots indicated a significant enrichment of FGFR1 in the nuclear matrix fraction...
- The PML gene encodes a phosphoprotein associated with the nuclear matrixK S Chang
Department of Hematology, University of Texas M D Anderson Cancer Center, Houston 77030, USA
Blood 85:3646-53. 1995..In K562 and NIH/3T3 cells transfected with a PML expression plasmid, we found PML to be associated with the nuclear matrix. Our results also showed that PML is a phosphorprotein...
- The sperm nucleus: chromatin, RNA, and the nuclear matrixGraham D Johnson
The Center for Molecular Medicine and Genetics, C S Mott Center, Wayne State University of Medicine, 275 East Hancock, Detroit, MI 48201, USA
Reproduction 141:21-36. 2011..These regions may be necessary for organizing higher order genomic structure through interactions with the nuclear matrix. The promoters of this transcriptionally quiescent genome are differentially marked by modified histones that ..
- Cyclin E is recruited to the nuclear matrix during differentiation, but is not recruited in cancer cellsJennifer Munkley
Department of Biology, University of York, YO10 5YW and School of Medicine, Leeds University, LS2 9JT, UK
Nucleic Acids Res 39:2671-7. 2011..We show that cyclin E, but not A, is mounted upon the nuclear matrix in sub-nuclear foci in differentiated vertebrate cells, but not in undifferentiated cells or cancer cells...
- Proto-oncoprotein TLS/FUS is associated to the nuclear matrix and complexed with splicing factors PTB, SRm160, and SR proteinsMichael Meissner
Institute of Cancer Research, Borschkegasse 8a, University of Vienna, A 1090 Vienna, Austria
Exp Cell Res 283:184-95. 2003..Here we report the association of TLS/FUS with the nuclear matrix and investigate its role in splicing...
- Accumulation of LANA at nuclear matrix fraction is important for Kaposi's sarcoma-associated herpesvirus replication in latencyEriko Ohsaki
Department of Infectious Diseases, University of Hamamatsu School of Medicine, 1 20 1 Handayama, Higashi ku, Hamamatsu, Shizuoka 431 3192, Japan
Virus Res 139:74-84. 2009..the KSHV genome, and a viral replication factor, latency-associated nuclear antigen (LANA) accumulate at the nuclear matrix fraction in the G1 phase...
- S/MARt DB: a database on scaffold/matrix attached regionsInes Liebich
Research Group Bioinformatics, GBF, Mascheroder Weg 1, D 38124 Braunschweig, Germany
Nucleic Acids Res 30:372-4. 2002..S/MAR transaction database, is a relational database covering scaffold/matrix attached regions (S/MARs) and nuclear matrix proteins that are involved in the chromosomal attachment to the nuclear scaffold...
- Structural insights into SUN-KASH complexes across the nuclear envelopeWenjia Wang
State Key Laboratory of Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China
Cell Res 22:1440-52. 2012..These results provide a structural model of the LINC complex, which is essential for additional study of the physical and functional coupling between the cytoplasm and the nucleoplasm...
- Function of the sperm nuclear matrixJeffrey A Shaman
Institute for Biogenesis Research, John A Burns School of Medicine, University of Hawaii Honolulu, HI 96822, USA
Arch Androl 53:135-40. 2007..One of these is the organization of DNA into loop domains attached at their bases to a proteinaceous nuclear matrix. Several groups have shown that the sites at which DNA associates with the sperm nuclear matrix contain ..
- Localization of prohibitin in the nuclear matrix and alteration of its expression during differentiation of human neuroblastoma SK-N-SH cells induced by retinoic acidQi fu Li
The Key Laboratory of Ministry of Education for Cell Biology and Tumor Cell Engineering, School of Life Science, Xiamen University, China
Cell Mol Neurobiol 31:203-11. 2011The nuclear matrix-intermediate filament system of human neuroblastoma SK-N-SH cells before and after retinoic acid (RA) treatment was selectively extracted and the distribution of prohibitin (PHB) in the nuclear matrix, as well as its ..
- Exploring the relationship between interphase gene positioning, transcriptional regulation and the nuclear matrixLauren S Elcock
Laboratory of Nuclear and Genomic Health, Centre for Cell and Chromosome Biology, Brunel University, Uxbridge UB8 3PH, UK
Biochem Soc Trans 38:263-7. 2010..at a more local scale? During the course of this review, these questions will be considered, in light of the current literature regarding the role of transcription factories and the nuclear matrix in interphase genome organization.
- DNA damage-dependent interaction of the nuclear matrix protein C1D with Translin-associated factor X (TRAX)Tuba Erdemir
Bilkent University, Molecular Biology and Genetics Department, 06533, Bilkent, Ankara, Turkey
J Cell Sci 115:207-16. 2002The nuclear matrix protein C1D is an activator of the DNA-dependent protein kinase (DNA-PK), which is essential for the repair of DNA double-strand breaks (DSBs) and V(D)J recombination...
- Aged and post-mitotic cells share a very stable higher-order structure in the cell nucleus in vivoJaneth Alva-Medina
Universidad Autonoma del Estado de Mexico, Toluca, Estado de Mexico, Mexico
Biogerontology 11:703-16. 2010..cells the nuclear DNA is organized in supercoiled loops anchored to a proteinaceous substructure known as the nuclear matrix (NM). The DNA-NM interactions define a higher-order structure in the cell nucleus (NHOS)...
- Hcc-1 is a novel component of the nuclear matrix with growth inhibitory functionC L Leaw
Bioprocessing Technology Institute, Agency for Science, Technology and Research A STAR, Singapore
Cell Mol Life Sci 61:2264-73. 2004..0173) and an accumulation of cells at the G2/M phase (p = 0.0276 compared to control HEK293 cells). Taken together, these results suggest a role for Hcc-1 in growth regulation and nucleic acids metabolism...
- A new spectrin, beta IV, has a major truncated isoform that associates with promyelocytic leukemia protein nuclear bodies and the nuclear matrixW T Tse
Division of Hematology Oncology, Children s Hospital, and the Dana Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
J Biol Chem 276:23974-85. 2001..Spectrin betaIV is the first beta-spectrin associated with a subnuclear structure and may be part of a nuclear scaffold to which gene regulatory machinery binds...
- A structural basis for cellular senescenceArmando Aranda-Anzaldo
Laboratorio de Biologia Molecular, Facultad de Medicina, Universidad Autonoma del Estado de Mexico, Paseo Tollocan y Jesús Carranza, Toluca, Edo Mex, Mexico
Aging (Albany NY) 1:598-607. 2009..and discuss evidence that the interactions between DNA and the nuclear substructure, commonly known as the nuclear matrix, define a higher-order structure within the cell nucleus that following thermodynamic constraints, ..
- Order and disorder in the nucleusWallace F Marshall
Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, Connecticut 06520, USA
Curr Biol 12:R185-92. 2002....
- NeuN/Fox-3 is an intrinsic component of the neuronal nuclear matrixMyrna A R Dent
Facultad de Medicina, Universidad Autonoma del Estado de Mexico, Toluca, Mexico, Mexico
FEBS Lett 584:2767-71. 2010..Here we provide evidence that NeuN/Fox-3 is an intrinsic component of the neuronal nuclear matrix and a reliable marker of nuclear speckles in neurons.
- Nuclear structure in cancer cellsDaniele Zink
University of Munich LMU, Department of Biology II, Goethestr 31, 80336 Munich, Germany
Nat Rev Cancer 4:677-87. 2004..Advances in understanding nuclear structure have revealed insights into the process of malignant transformation and provide a basis for the development of new diagnostic tools and therapeutics...
- Silencing by nuclear matrix attachment distinguishes cell-type specificity: association with increased proliferation capacityAmelia K Linnemann
Department of Obstetrics and Gynecology, The Center for Molecular Medicine and Genetics, Wayne State University School of Medicine, Detroit, MI 48201, USA
Nucleic Acids Res 37:2779-88. 2009..We have previously shown that attachment of genes to the NaCl-isolated nuclear matrix correlates with their silencing in HeLa cells...
- The cerebellar leucine-rich acidic nuclear protein interacts with ataxin-1A Matilla
Department of Pediatrics, Baylor College of Medicine, Houston, Texas 77030, USA
Nature 389:974-8. 1997..Immunofluorescence studies demonstrate that both LANP and ataxin-1 colocalize in nuclear matrix-associated subnuclear structures...
- CpG-binding protein is a nuclear matrix- and euchromatin-associated protein localized to nuclear speckles containing human trithorax. Identification of nuclear matrix targeting signalsJeong Heon Lee
Herman B Wells Center for Pediatric Research, Section of Pediatric Hematology Oncology, Department of Pediatrics and Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis 46202, USA
J Biol Chem 277:42259-67. 2002..CGBP associates with the nuclear matrix, and fragments of CGBP that fail to associate with the nuclear matrix fail to localize to nuclear speckles and ..
- Experimental observations of a nuclear matrixJ Nickerson
Department of Cell Biology and Cancer Center, University of Massachusetts Medical School, Worcester, MA 01655, USA
J Cell Sci 114:463-74. 2001..architecture of the nucleus includes two overlapping and nucleic-acid-containing structures - chromatin and a nuclear matrix. The nuclear matrix is observed by microscopy in live, fixed and extracted cells...
- The amino-terminal region of the retinoblastoma gene product binds a novel nuclear matrix protein that co-localizes to centers for RNA processingT Durfee
Center for Molecular Medicine Institute of Biotechnology, University of Texas Health Science Center at San Antonio 78245
J Cell Biol 127:609-22. 1994..The only gene isolated from this screen encodes a novel 84-kD nuclear matrix protein that localizes to subnuclear regions associated with RNA processing...
- Ataxin-3 is transported into the nucleus and associates with the nuclear matrixD Tait
Max Planck Institut fur Molekulare Genetik, Ihnestrasse 73, D 14195 Berlin Dahlem, Germany
Hum Mol Genet 7:991-7. 1998..revealed that the ataxin-3 protein present in the nucleus of neuroblastoma cells is associated with the inner nuclear matrix. Our results taken together with the finding of a nuclear localization signal in ataxin-3 indicate that the ..
- Nuclear matrix interactions within the sperm genomeJ A Kramer
Department of Obstetrics and Gynecology, Center for Molecular Medicine and Genetics, Wayne State University School of Medicine, Detroit, Michigan 48102, USA
J Biol Chem 271:11619-22. 1996..human PRM1 --> PRM2 --> TNP2 genic domain has revealed two regions of attachment to the sperm nuclear matrix. These sperm nuclear matrix attachment regions delimit the DNase I-sensitive domain of this haploid-expressed ..
- A new look at the nuclear matrixR Hancock
Centre de recherche en cancérologie de l Université Laval, Hotel Dieu Hospital, Quebec, Canada
Chromosoma 109:219-25. 2000The concept of the nuclear matrix, a karyoskeletal structure that serves as a support for the genome and its activities, has stimulated many studies of the association of nuclear components and functions with this structure...
- Cloning and expression of a novel nuclear matrix-associated protein that is regulated during the retinoic acid-induced neuronal differentiationW M Cheung
Department of Biochemistry, Biotechnology Research Institute, and Molecular Neuroscience Center, Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong, China
J Biol Chem 276:17083-91. 2001..primed polymerase chain reaction, we identified a novel serine/threonine-rich protein, RA-regulated nuclear matrix-associated protein (Ramp), that was down-regulated during the RA-induced differentiation of NT2 cells...
- Characterization of SAF-A, a novel nuclear DNA binding protein from HeLa cells with high affinity for nuclear matrix/scaffold attachment DNA elementsH Romig
Department of Biology, University of Konstanz, Germany
EMBO J 11:3431-40. 1992..SAF-A is an abundant nuclear protein and a constituent of the nuclear matrix and scaffold...
- Interaction of EBV latent origin of replication with the nuclear matrix: identification of S/MAR sequences and protein componentsGiulia Mearini
Department of Public Health Sciences, University La Sapienza, Rome, Italy
FEBS Lett 547:119-24. 2003During latency, Epstein Barr virus (EBV) genome, as an episome, is attached to the nuclear matrix (NM) via the latent origin of replication ori P...
- DNA moves sequentially towards the nuclear matrix during DNA replication in vivoJuan Carlos Rivera-Mulia
Laboratorio de Biologia Molecular, Facultad de Medicina, Universidad Autonoma del Estado de Mexico, Apartado Postal 428, CP 50000 Toluca, Edo Mex, Mexico
BMC Cell Biol 12:3. 2011In the interphase nucleus of metazoan cells DNA is organized in supercoiled loops anchored to a nuclear matrix (NM)...
- Unraveling the organization of the internal nuclear matrix: RNA-dependent anchoring of NuMA to a lamin scaffoldPaola Barboro
Istituto Nazionale per la Ricerca sul Cancro, I 16132, Genova, Italy
Exp Cell Res 279:202-18. 2002Using quantitative immunoelectron microscopy we show here that when the nuclear matrix is isolated from rat hepatocytes in the presence of an inhibitor of RNase activity both lamins and the nuclear mitotic apparatus protein (NuMA) ..
- Mapping of the nuclear matrix-bound chromatin hubs by a new M3C experimental procedureAlexey A Gavrilov
Laboratory of Structural and Functional Organization of Chromosomes, Institute of Gene Biology of the Russian Academy of Sciences, 34 5 Vavilov Street, 119334 Moscow, Russia
Nucleic Acids Res 38:8051-60. 2010We have developed an experimental procedure to analyze the spatial proximity of nuclear matrix-bound DNA fragments...
- Sp2 localizes to subnuclear foci associated with the nuclear matrixK Scott Moorefield
Graduate Program in Genomic Sciences, Center for Comparative Medicine and Translational Research, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27606, USA
Mol Biol Cell 17:1711-22. 2006..We report that 1) Sp2 localizes largely within subnuclear foci associated with the nuclear matrix, and 2) these foci are distinct from promyelocytic oncogenic domains and appear to be stable during an 18-h ..
- Nuclear matrix association of insulin receptor and IRS-1 by insulin in osteoblast-like UMR-106 cellsKi Cheon Seol
Department of Pharmacology, School of Dentistry, Kyung Hee University, Seoul 130 701, Republic of Korea
Biochem Biophys Res Commun 306:898-904. 2003..The majority of insulin receptor and IRS-1 translocated to the nucleus appeared to associate with nuclear matrix. Tyrosine phosphorylation of a number of proteins with a molecular mass of 180, 95, 85, or 70 kDa in the ..
- The newly identified human nuclear protein NXP-2 possesses three distinct domains, the nuclear matrix-binding, RNA-binding, and coiled-coil domainsYukio Kimura
Department of Life Science, Graduate School and Faculty of Science, Himeji Institute of Technology, Kamigori, Hyogo 678 1201, Japan
J Biol Chem 277:20611-7. 2002Using a monoclonal antibody that recognizes a nuclear matrix protein, we selected a cDNA clone from a lambdagt11 human placenta cDNA library. This cDNA encoded a 939-amino acid protein designated nuclear matrix protein NXP-2...
- c-Abl tyrosine kinase selectively regulates p73 nuclear matrix associationMerav Ben-Yehoyada
Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
J Biol Chem 278:34475-82. 2003..that in response to ionizing radiation, p73 undergoes nuclear redistribution and becomes associated with the nuclear matrix. This association is c-Abl-dependent because it was not observed in cells that are defective in c-Abl kinase ..
- Interactions of Epstein-Barr virus origins of replication with nuclear matrix in the latent and in the lytic phases of viral infectionE Mattia
Faculty of Medicine and Surgery, University of Roma La Sapienza, Rome, Italy
Virology 262:9-17. 1999Eukaryotic DNA is organized into domains or loops generated by the attachment of chromatin fibers to the nuclear matrix via specific regions called scaffold or matrix attachment regions...
- Gene positional changes relative to the nuclear substructure correlate with the proliferating status of hepatocytes during liver regenerationApolinar Maya-Mendoza
Laboratorio de Biologia Molecular, Facultad de Medicina, Universidad Autonoma del Estado de Mexico, Apartado Postal 428, C P 50000, Toluca, Edo Mex, Mexico
Nucleic Acids Res 31:6168-79. 2003..is organised in loops anchored to a proteinaceous substructure variously named but commonly known as the nuclear matrix. Important processes of nuclear physiology, such as replication, transcription and processing of primary ..
- Autosomal-dominant distal myopathy associated with a recurrent missense mutation in the gene encoding the nuclear matrix protein, matrin 3Jan Senderek
Institute of Cell Biology, ETH Zurich, 8093 Zurich, Switzerland
Am J Hum Genet 84:511-8. 2009..MATR3 is expressed in skeletal muscle and encodes matrin 3, a component of the nuclear matrix, which is a proteinaceous network that extends throughout the nucleus...
- Re-defining the chromatin loop domainH H Heng
Center for Molecular Medicine and Genetics, Department of Pathology, Karmanos Cancer Institute, Wayne State University School of Medicine, Detroit MI 48202, USA
Cytogenet Cell Genet 93:155-61. 2001..to target some long-standing issues regarding the structure and function of the chromatin loop domain and its relationship with the nuclear matrix. This new knowledge will have a profound impact for modern genetics and molecular medicine.
- Boundary element-associated factor 32B connects chromatin domains to the nuclear matrixRashmi U Pathak
Department of Biochemistry, School of Life Sciences, University of Hyderabad, Hyderabad, India
Mol Cell Biol 27:4796-806. 2007..In this report we show that BEAF is associated with the nuclear matrix and map the domain required for matrix association to the middle region of the protein...
- Gene positional changes relative to the nuclear substructure during carbon tetrachloride-induced hepatic fibrosis in ratsApolinar Maya-Mendoza
Laboratorio de Biologia Molecular, Facultad de Medicina, Universidad Autonoma del Estado de Mexico, Apdo Postal 428, C P 50000, Toluca, Edo Mex, Mexico
J Cell Biochem 93:1084-98. 2004..interphase nucleus the DNA of higher eukaryotes is organized in loops anchored to a substructure known as the nuclear matrix (NM)...
- Exploring the effects of a dysfunctional nuclear matrixLauren S Elcock
Laboratory of Nuclear and Genomic Health, Centre for Cell and Chromosome Biology, Brunel University, Uxbridge, UK
Biochem Soc Trans 36:1378-83. 2008The nuclear matrix has remained a contentious structure for decades; many believe that it is an artefact of harsh non-physiological procedures...
- Initiation of DNA replication at a nuclear matrix-attached chromatin fractionIlian Radichev
Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia, Bulgaria
J Cell Physiol 203:71-7. 2005..To address this issue, we developed a cell-free replication system in which the nuclear matrix along with the residual matrix-attached chromatin was used as a substrate for DNA replication...
- Differential nuclear scaffold/matrix attachment marks expressed genesAmelia K Linnemann
The Center for Molecular Medicine and Genetics, Wayne State University School of Medicine, C S Mott Center, Detroit, MI48201, USA
Hum Mol Genet 18:645-54. 2009..The varied functions of the S/MARs as revealed by the different extraction methods highlights their unique functional contribution...
- Localization and altered expression of nucleophosmin in the nuclear matrix during the differentiation of human hepatocarcinoma SMMC-7721 cells induced by HMBAQi fu Li
The Key Laboratory of Ministry of Education for Cell Biology and Tumor Cell Engineering, School of Life Sciences, Xiamen University, Xiamen, PR China
Cancer Invest 28:1004-12. 2010..To investigate the mechanism of induced differentiation of tumor cells, the nuclear matrix of human hepatocarcinoma SMMC-7721 cells induced by hexamethylene bisacetamide (HMBA) was selectively ..
- Age-related association of rDNA and telomeres with the nuclear matrix in mouse hepatocytesAlberto S Moraes
Department of Cell Biology, Institute of Biology, State University of Campinas, 13083 863 Campinas, Sao Paulo, Brazil
Cell Biol Int 34:925-31. 2010Transcribed sequences have been suggested to be associated with the nuclear matrix, differing from non-transcribing sequences, which have been reported to be contained in DNA loops...
- Characterization of a novel Dp71 dystrophin-associated protein complex (DAPC) present in the nucleus of HeLa cells: members of the nuclear DAPC associate with the nuclear matrixLizeth Fuentes-Mera
Department of Genetics and Molecular Biology, Centro de Investigacion y de Estudios Avanzados del IPN, Avenida Instituto Politecnico Nacional 2508, Apartado Postal 14 740, C P 07000, Mexico D F, Mexico
Exp Cell Res 312:3023-35. 2006..Next, we analyze the possible association of the nuclear DAPC with the nuclear matrix. We found the presence of Dp71, beta-dystroglycan, nNOS, beta-sarcoglycan, alpha/beta syntrophin, alpha1-..
- A-kinase-anchoring protein AKAP95 is targeted to the nuclear matrix and associates with p68 RNA helicaseL Akileswaran
Neurological Sciences Institute, Oregon Health Sciences University, Beaverton, Oregon 97006, USA
J Biol Chem 276:17448-54. 2001The cell nucleus is structurally and functionally organized by the nuclear matrix. We have examined whether the nuclear cAMP-dependent protein kinase-anchoring protein AKAP95 contains specific signals for targeting to the subnuclear ..
- Sequences attaching loops of nuclear and mitochondrial DNA to underlying structures in human cells: the role of transcription unitsD A Jackson
CRC Nuclear Structure and Function Research Group, Sir William Dunn School of Pathology, University of Oxford, UK
Nucleic Acids Res 24:1212-9. 1996..These results are consistent with transcription being responsible for ever-changing attachments in both nuclei and mitochondria...
- Interaction of the nuclear matrix protein NAKAP with HypA and huntingtin: implications for nuclear toxicity in Huntington's disease pathogenesisJonathan A Sayer
Neurological Sciences Institute, Oregon Health and Science University, Beaverton, OR 97006, USA
Neuromolecular Med 7:297-310. 2005..In cultured cells, NAKAP and HypA localize within the nucleus and copurify with the nuclear matrix. Furthermore, NAKAP associates with HypA from human brain and copurifies with huntingtin protein in brain ..
- Emp is a component of the nuclear matrix of mammalian cells and undergoes dynamic rearrangements during cell divisionShashi Bala
Center of Cell Biology, Department of Medicine, Caritas St Elizabeth s Medical Center, Tufts University School of Medicine, Boston, MA 02135, USA
Biochem Biophys Res Commun 342:1040-8. 2006..Sequential extractions of interphase nuclei showed that recombinant Emp was present predominantly in the nuclear matrix. Immunofluorescence microscopy showed that Emp was present in typical nuclear speckles enriched with the ..
- Nuclear matrix protein SMAR1 represses HIV-1 LTR mediated transcription through chromatin remodelingKadreppa Sreenath
National Centre for Cell Science, University of Pune Campus, Ganeshkhind, Pune 411007, Maharashtra, India
Virology 400:76-85. 2010b>Nuclear Matrix and MARs have been implicated in the transcriptional regulation of host as well as viral genes but their precise role in HIV-1 transcription remains unclear...
- Nuclear matrix proteome analysis of Drosophila melanogasterSatish Kallappagoudar
Centre for Cellular and Molecular Biology, Council of Scientific and Industrial Research, Hyderabad, India
Mol Cell Proteomics 9:2005-18. 2010..is unknown, a major component of this organization is likely to be the non-chromatin scaffolding called nuclear matrix (NuMat)...
- Drosophila dSAP18 is a nuclear protein that associates with chromosomes and the nuclear matrix, and interacts with pinin, a protein factor involved in RNA splicingElisabet Costa
Departament de Biologia Molecular i Cellular, Institut de Biologia Molecular de Barcelona IBMB CSIC, Parc Cientific de Barcelona, Josep Samitier 1 5, 08028, Barcelona, Spain
Chromosome Res 14:515-26. 2006..that, in Drosophila, dSAP18 is a predominantly nuclear protein that associates to both chromosomes and the nuclear matrix. dSAP18 becomes nuclear early during development, at the onset of cellularization, and remains so all through ..
- Matrix attachment regions and structural colinearity in the genomes of two grass speciesZ Avramova
Department of Biological Sciences and Purdue Genetics Program, Purdue University, West Lafayette, IN 47907, USA
Nucleic Acids Res 26:761-7. 1998....
- TRiC-P5, a novel TCP1-related protein, is localized in the cytoplasm and in the nuclear matrixE C Joly
Institut du Cancer de Montreal, Centre de Recherche Louis Charles Simard, Quebec, Canada
J Cell Sci 107:2851-9. 1994..In addition, a significant part of TRiC-P5 is also found in the nucleus where it is attached to the nuclear matrix, a complex filament network involved in essential cellular functions such as DNA replication, and RNA ..
- Analysis of sequence-dependent curvature in matrix attachment regionsJ Yamamura
Institute of Agriculture and Forestry, University of Tsukuba, Ibaraki 305 8572, Tsukuba, Japan
FEBS Lett 489:166-70. 2001..These results suggest that DNAs of MARs have a characteristic conformation instead of conserved sequence...
- Regulating the mammalian genome: the role of nuclear architectureRonald Berezney
Department of Biological Sciences, State University of New York at Buffalo, 14260, USA
Adv Enzyme Regul 42:39-52. 2002
- Impaired nuclear translocation, nuclear matrix targeting, and intranuclear mobility of mutant androgen receptors carrying amino acid substitutions in the deoxyribonucleic acid-binding domain derived from androgen insensitivity syndrome patientsHisaya Kawate
Department of Geriatric Medicine, Graduate School of Medical Sciences, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
J Clin Endocrinol Metab 90:6162-9. 2005..In this study, we examined whether intracellular dynamics of mutant ARs detected in two androgen insensitivity syndrome (AIS) patients was impaired...
- The KRAB zinc finger gene ZNF74 encodes an RNA-binding protein tightly associated with the nuclear matrixB Grondin
Institut de Recherches Cliniques de Montreal, Universite de Montreal, Montreal, Quebec H2W 1R7, Canada
J Biol Chem 271:15458-67. 1996..Subcellular and in situ sequential fractionations further showed that ZNF74 is associated with the nuclear matrix. The RNA binding properties of this protein and its tight association with the nuclear matrix, a subnuclear ..
- DNA-PK-dependent binding of DNA ends to plasmids containing nuclear matrix attachment region DNA sequences: evidence for assembly of a repair complexStanley K Mauldin
Lankenau Institute for Medical Research, 100 Lancaster Avenue, Wynnewood, PA 19096, USA and Genisphere, Incorporated, 4170 City Avenue, Philadelphia, PA 19131 1694, USA
Nucleic Acids Res 30:4075-87. 2002..Competition experiments between pUC18 and pUC18 plasmids containing various nuclear matrix attachment region (MAR) sequences suggest that DNA ends preferentially associate with plasmids containing MAR ..
- A nuclear matrix attachment region upstream of the T cell receptor beta gene enhancer binds Cux/CDP and SATB1 and modulates enhancer-dependent reporter gene expression but not endogenous gene expressionS Chattopadhyay
Center for Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
J Biol Chem 273:29838-46. 1998..Furthermore, we demonstrate that HS1 is a nuclear matrix attachment region, referred to as MARbeta...
- DNA replication initiates at domains overlapping with nuclear matrix attachment regions in the xenopus and mouse c-myc promoterClaire Girard-Reydet
Institute of Human Genetics, CNRS, Genome Dynamics and Development, 141, rue de la Cardonille, 34396 Montpellier Cedex 5, France
Gene 332:129-38. 2004..We also identified a nuclear matrix attachment region in both Xenopus and mouse, which is localized to two different regions of the c-myc promoter ..
- The homeodomain coordinates nuclear entry of the Lhx3 neuroendocrine transcription factor and association with the nuclear matrixG E Parker
Department of Biology, Indiana University Purdue University, Indianapolis, Indiana 46202 5132, USA
J Biol Chem 275:23891-8. 2000..By using functional fluorescent derivatives, we demonstrate that Lhx3 is found in both the nucleoplasm and nuclear matrix. Three nuclear localization signals were mapped within the homeodomain, and one was located in the carboxyl ..
- p53 binds the nuclear matrix in normal cells: binding involves the proline-rich domain of p53 and increases following genotoxic stressM Jiang
YCR P53 Research Group, Department of Biology, University of York, YO10 5DD, UK
Oncogene 20:5449-58. 2001..In mammalian cells the biochemical processing of DNA occurs on a nuclear sub-structure termed the nuclear matrix. Previously Deppert and co-workers have identified p53 in association with the nuclear matrix in viral- and ..
- Stabilization of the nuclear matrix by disulfide bridges: identification of matrix polypeptides that form disulfidesN Stuurman
E C Slater Institute for Biochemical Research, University of Amsterdam, The Netherlands
Exp Cell Res 200:285-94. 1992The molecular structure of the nuclear matrix is still poorly understood. We have tried to assess which proteins are important structural elements by examining the process of stabilization of the nuclear matrix by sodium tetrathionate...
- Nuclear matrix protein mitotin messenger RNA is expressed at constant levels during the cell cycleI T Todorov
Institut of Cell Biology and Morphology, Bulgarian Academy of Sciences, Sofia
Biochem Biophys Res Commun 177:395-400. 1991During the course of an investigation on nuclear matrix protein cDNAs, we have isolated a cDNA clone hybridizing with the messenger RNA encoding mitotin. Mitotin is a 125 kDa/pI 6...
- Phosphorylation of AML1/RUNX1 regulates its degradation and nuclear matrix associationJoseph R Biggs
Department of Medicine, P O Box 250955, Medical University of South Carolina, 6 Jonathan Lucas Street, Charleston, SC 29425, USA
Mol Cancer Res 3:391-401. 2005..one soluble in buffers containing salt and nonionic detergent and the other insoluble and tightly bound to the nuclear matrix. We find that the AML1c protein is modified by both phosphorylation and ubiquitination...
- Interaction of the tau2 transcriptional activation domain of glucocorticoid receptor with a novel steroid receptor coactivator, Hic-5, which localizes to both focal adhesions and the nuclear matrixL Yang
Department of Pathology and Department of Biochemistry and Molecular Biology, University of Southern California, Los Angeles, California 90089, USA
Mol Biol Cell 11:2007-18. 2000..identified Hic-5 as a protein that interacts with a region of the glucocorticoid receptor that includes a nuclear matrix-targeting signal and the tau2 transcriptional activation domain...
- A possible role of cholesterol-sphingomyelin/phosphatidylcholine in nuclear matrix during rat liver regenerationElisabetta Albi
Department of Biochemical Sciences and Molecular Biotechnology, Physiopathology, Policlinico Monteluce, 06100 Perugia, Italy
J Hepatol 38:623-8. 2003..The aim of the present work is to clarify if chromatin lipids may derive or not from nuclear matrix and if they have different roles.
- The controversial nuclear matrix: a balanced point of viewA M Martelli
Dipartimento di Scienze Anatomiche Umane e Fisiopatologia dell Apparato Locomotore, Sezione di Anatomia, Universita di Bologna, Bologna, Italy
Histol Histopathol 17:1193-205. 2002The nuclear matrix is defined as the residual framework after the removal of the nuclear envelope, chromatin, and soluble components by sequential extractions...
- DNA binding and gene activation properties of the Nmp4 nuclear matrix transcription factorsKitti Torrungruang
Department of Periodontics, Indiana University School of Dentistry, Indianapolis, Indiana 46202, USA
J Biol Chem 277:16153-9. 2002Splice variants of the Nmp4 gene include nuclear matrix transcription factors that regulate the type I collagen alpha1(I) polypeptide chain (COL1A1) promoter and several matrix metalloproteinase (MMP) genes...
- Co-localization of poly(ADPR)polymerase 1 (PARP-1) poly(ADPR)polymerase 2 (PARP-2) and related proteins in rat testis nuclear matrix defined by chemical cross-linkingFilomena Tramontano
Department of Biological Chemistry, University Federico II of Naples, Via Mezzocannone 16, 80134 Napoli, Italy
J Cell Biochem 94:58-66. 2005..Moreover, the association of pADPR and PARP-1 with the nuclear matrix (NM) has been reported, based on the poly(ADP-ribosyl)ation of nuclear matrix proteins (NMPs)...
- Chymase-Mediated MMP Activation in Ishemia Reperfusion InjuryLOUIS JOSEPH DELL'ITALIA; Fiscal Year: 2013..Recent studies demonstrated activated MMP-2 within the cardiomyocyte that cleaved myofibrils (4-6) and nuclear matrix protein poly ADP-ribose polymerase (PARP)-a DNA repair enzyme in cell survival during I/R (7)...
- PAX2 gene targets, binding sites and functionPatrick D Brophy; Fiscal Year: 2010..Further experiments will focus on target gene association with nuclear matrix, which also is known to provide a change from random to specific anchorage points when chromatin becomes ..
- Checkpoints of TNF Gene RegulationAnne Goldfeld; Fiscal Year: 2013..DNase hypersensitivity sites (HSSs), intrachromosomal interactions, and interactions with the nuclear matrix. The identification of potential therapeutic targets to control TNF expression in specific cell types and in ..
- Contribution of Sperm Nucleus to Paternal DNA ReplicationWILLIAM S WARD; Fiscal Year: 2013..In Specific Aim 3 we will test whether ORC2L-5L or ORC1L binds directly to the sperm nuclear matrix, using ORC-GFP fusion proteins...
- Repair of oxidative damage in mammalian genomesTapas K Hazra; Fiscal Year: 2010..mutations in NEIL2-deficient cells, are consistent with this hypothesis, hnRNP-U, an abundant multifunctional nuclear matrix protein, has also been shown to regulate transcription. NEIL2 carries out a beta-delta-reaction like E...
- Elucidating the role of TIN2L at the telomere and in dyskeratosis congenitaNya D Nelson; Fiscal Year: 2013..TIN2L associates with the telomeres and the nuclear matrix, indicating that it is unlikely to have identical function to TIN2S...
- A TRANSCRIPTION FACTOR WITHIN LIPID RAFTS MODULATES B CELL SIGNALING VIA THE BCRPhilip W Tucker; Fiscal Year: 2010..transcription factor that transactivates the immunoglobulin heavy chain (IgH) locus by binding to nuclear matrix attachment regions flanking the IgH intronic enhancer...
- Epigenetic Regulation in Self-Renewing and Differentiating Male Germ CellsCHRISTOPHER JESS PAYNE; Fiscal Year: 2012..The second aim will examine the role of nuclear matrix proteins in the maintenance of selfrenewing spermatogonia...
- Functional Analysis of the Hairless ProteinAngela M Christiano; Fiscal Year: 2012..We also demonstrated that Hr is tightly associated with the nuclear matrix and forms nuclear speckles in association with the histone deacetylase, HDAC3...
- Anaplasma regulation of host granulocyte functionJohn Stephen Dumler; Fiscal Year: 2013..reprograms the global neutrophil transcriptome by altering the epigenome through AnkA"s action on nuclear matrix, chromatin, and transcriptional apparatus recruitment. We propose the following aims: 1...
- Understanding the Regulation of Megakaroblastic Leukemia 1 (MLK1)THERA C ALEXIS; Fiscal Year: 2010..SAP region's function is not well understood but has been involved in nuclear matrix attachment in other proteins...
- KSHV-Ori-Lyt-Dependent DNA ReplicationYan Yuan; Fiscal Year: 2012..Two cellular proteins that may involve origin DNA unwinding and nuclear matrix attachment will be further studied because these two events are believed to be antecedent steps before ..
- Mechanism &Function of Subnuclear Targeting of Transcription Factors in BoneGary S Stein; Fiscal Year: 2011..Our program initially identified Runx2 as the osteoblast-specific nuclear matrix protein NMP2...
- Boston University Cross-Disciplinary Training in Nanotechnology for CancerBennett B Goldberg; Fiscal Year: 2013..Proteomics in particular is emerging as a tool for detection of nuclear matrix proteins and new biomarkers for screening of early tumors stage...
- Serum Proteomic Biomarkers for Colon & Pancreatic CancerWilliam Bigbee; Fiscal Year: 2009..Two major Projects are proposed: (1) Nuclear Matrix Proteins for Early Detection of CRC, and (2) Luminex Multianalyte Profiling (LabMAP(r)) and SELDI-TOF-MS Serum ..
- Preclinical assay development of a novel Colon Cancer Marker (CCSA-4)JACK CLEMENS; Fiscal Year: 2007..Onconome, Inc. has licensed these novel nuclear matrix proteins and is developing products for the detection of these biomarkers for research and clinical use...
- Refinement and Discovery of Nuclear Matrix Protein Markers for Colorectal CancerRobert E Schoen; Fiscal Year: 2013..applicant): The focus of this proposal is to continue development of a unique panel of colon cancer associated nuclear matrix proteins (NMPs), discovered and developed during our previous EDRN-sponsored, Biomarker Development Laboratory ..
- ANTIMETOBOLITES AND NUCLEAR MATRIX-BOUND DNA REPLICATIONDaniel Fernandes; Fiscal Year: 1991..Recent evidence supports the concept that in eucaryotic cells DNA replication are attached to fixed sites on the nuclear matrix, and that template DNA passes through these sites as it is replicated...
- ROLE OF TESTIS-SPECIFIC HISTONES IN SPERMATOGENESISChi Bom Chae; Fiscal Year: 1993..TH2B genes, presence of testis-specific dominant control region near the domain, definition of the domain by nuclear matrix binding sites, and regulation of testis-specific histone genes by the testis-specific enhancer activity of the ..
- CELLULAR PROCESSING OF DAMAGED DNA--ROLE IN ONCOGENESISPhilip Hanawalt; Fiscal Year: 2000..b>Nuclear matrix associated DNA will be characterized to determine whether that is the site of transcription-coupled repair...
- AUTOANTIBODIES TO CELLULAR MATRIX ANTIGENS IN CFSENG TAN; Fiscal Year: 2000..Cellular structures (nuclear envelope, vimentin-containing intermediate filaments and a nuclear matrix particle visualized in immunofluorescence as reticulated speckles) associated with these antigens contain ..
- Proteomic Characterization of IC BladderPradeep Tyagi; Fiscal Year: 2006..The nuclear matrix is the structural scaffolding of the cell nucleus and plays a central role in the regulation of important ..
- ANTIBODIES TO NUCLEAR PROTEINS IN CERVICAL CANCEREDWARD FEY; Fiscal Year: 1992..We have fractionated Pap smear samples and purified nuclear matrix proteins from normal patients and from patients with carcinoma in situ...
- CHROMATIN-TELOMERE STRUCTURE AND AT GENOMIC SENSITIVITYTEJ PANDITA; Fiscal Year: 2005..We demonstrated that altered telomere nuclear matrix interactions and nucleosomal periodicity observed in cells derived from individuals with ataxia telangiectasia ..
- MATRIX ATTACHMENT REGIONS IN AN IMPRINTED DOMAINJohn Greally; Fiscal Year: 2001..MARs are DNA sequences which anchor chromatin to a fixed nuclear matrix. Many nuclear factors (such as transcriptional and replication enzymes) are associated with the nuclear matrix,..
- L-TRIIODOTHYRONINE EFFECTS ON CELL REGULATIONMartin Surks; Fiscal Year: 1992..shown that T3 nuclear receptor concentration is augmented in S phase of the cell cycle and is localized to the nuclear matrix, a DNA and salt resistant nuclear fraction that is associated with new DNA synthesis and transcription ..
- NUTRITION AND PROTEIN METABOLISM IN TUMOR AND HOSTNawfal Istfan; Fiscal Year: 2000..The latter is thought to impact on the organization of the nuclear matrix which has been related to DNA replication origins through specific attachments (scaffold attachment regions, ..
- INTERACTION OF CIS-ACTING ELEMENTS IN CD8 REGULATIONPhilip Tucker; Fiscal Year: 2005..The L2a element is a nuclear matrix associated region (or MAR). Interestingly, inclusion of both the E81 enhancer and a 4...
- FUNCTION OF MATRIX ATTACHMENT REGIONS IN THE IG LOCIANN FEENEY; Fiscal Year: 2000Matrix attachment regions (MAR) are AT-rich regions of DNA which mediate attachment of the chromosome to the nuclear matrix. MARs have been studied in a few model loci, two of which are the IgH and Ig- kappa loci...
- HEAT AND RADIATION INDUCED CHANGES IN THE NUCLEAR MATRIXJOSEPH DYNLACHT; Fiscal Year: 2000The nuclear matrix is important for defining nuclear structure and regulating metabolic processes such as DNA and RNA synthesis, and possibly DNA repair...
- TOPOISOMERASE DRUG ACTIONS AT NUCLEAR MATRIX DNA DOMAINDaniel Fernandes; Fiscal Year: 2003DESCRIPTION: (Applicant's Abstract) The nuclear matrix model of DNA replication and organization will be utilized in the proposed studies to address (a) important effects of topoisomerase-active drugs on primary and higher order ..
- EFFECTS OF CHROMATIN ARCHITECTURE ON ORIGIN ACTIVITYPIETER DIJKWEL; Fiscal Year: 2003The long-range goal is to define the role of chromosome architecture, and in particular interaction with the nuclear matrix, in the regulation of replication origin activity in mammalian cells...
- The Fate of dsRNA in the NucleusGordon Carmichael; Fiscal Year: 2006..This complex contains the inosine-specific RNA binding protein p54nrb, the splicing factor PSF and the inner nuclear matrix structural protein matrin 3...
- CELLULAR MECHANISMS OF HYPERTHERMIA DAMAGERONALD COSS; Fiscal Year: 1992..These structural systems are: 1) the cytomatrix; 2) the nuclear envelope/lamina complex; and 3) the nuclear matrix. The major aim will be to test the hypothesis that unrepaired heat-induced structural damage to these systems ..
- Base unpairing regions and chromatin foldingTerumi Kohwi Shigematsu; Fiscal Year: 2004..to be accomplished by chromatin making periodic attachments to the residual framework of nuclei, called the nuclear matrix, at specialized DNA sequences called matrix attachment regions, or MARs...
- STUDIES OF NORMAL AND NEOPLASTIC PROSTATEKhalil Ahmed; Fiscal Year: 1993..of certain nuclear cAMP-independent PKs which may be involved in the phosphorylation of euchromatin NHP, nuclear matrix NHP, and androgen receptor...
- BIOCHEMISTRY OF LATE MITOTIC EVENTS IN CULTURED CELLSLON HODGE; Fiscal Year: 1980..phospholipid precursors to probe nuclear envelope reformation and by use of a defined nuclear subfraction, the nuclear matrix, of probe reformation of other nuclear structures...
- Nanopatterned Surfaces to Control Cell FateKEVIN HEALY; Fiscal Year: 2006..mechanical forces transmitted via the direct link of the cytoskeleton to the nucleus, and in particular to nuclear matrix proteins (NMPs) such as NMP 1 and 2...
- MOLECULAR MECHANISM OF MINERALOCORTICOID RECEPTOR ACTIONGeza Fejes Toth; Fiscal Year: 2003..Our studies also indicate that MR clusters are associated with the nuclear matrix but not with pallindromic hormone response elements...
- Molecular Genetic Study of Repair of Radiation DamageTHOMAS STAMATO; Fiscal Year: 2003..DNA breaks and interacts with DNA-PKcs/Ku to direct the association of DNA ends with MAR DNA sequences on the nuclear matrix. Aim 1 is to Investigate the involvement of the human PSO4 protein in DNA-PKcS/Ku mediated association of DNA ..
- INTRACELLULAR MECHANISMS OF GLUCOCORTICOID ACTIONDonald Defranco; Fiscal Year: 2005..ECM) effects on gene regulatory events are hypothesized to occur, in part, through a direct effect on the nuclear matrix. The role of the ECM in several biological processes is now well established and much is known about the ..
- REGULATION OF GLUCOCORTICOID RECEPTOR GENE EXPRESSIONWAYNE VEDECKIS; Fiscal Year: 2002..the: role that the transcription factor YY1 plays in regulating GR gene transcription; possible involvement of nuclear matrix association in this process; identification of the GR promoter sequences that regulate cell cycle dependent ..
- CELL-TYPE SPECIFIC SUPPRESSION OF THE IGH ENHANCERRichard Scheuermann; Fiscal Year: 1999..DNA binding and repressor function, and to investigate the functional interaction between NF-muNR and the nuclear matrix in transgenic mice...
- HOXBOXES, HOMEODOMAINS, AND OSTEOBLAST GENE EXPRESSIONDwight Towler; Fiscal Year: 2007..that enhances Runx2-dependent OC transcription; and (b) MINT (Msx2 interacting nuclear target), an RRM domain nuclear matrix protein that we discovered by interaction cloning...
- NON B DNA STRUCTURE WITH CHEMICAL CARCINOGENSTerumi Kohwi Shigematsu; Fiscal Year: 2001..It is believed that chromatin loops are anchored to the nuclear matrix at specific DNA regions (Matrix attachment regions, or MARs) that exhibit high affinity to the nuclear matrix...
- SPINAL CORD INJURY AND BLADDER CANCER DETECTIONRobert Getzenberg; Fiscal Year: 1999..The investigators have recently identified nuclear matrix proteins (NMPs) with which it is possible to differentiate human bladder tumors from normal bladder epithelial ..
- NUCLEAR CORE FILAMENTSSheldon Penman; Fiscal Year: 2001DESCRIPTION: (Adapted from applicant's abstract). Embedment-free electron microscopy shows the nuclear matrix comprised of a network of 11 nm filaments with numerous embedded dense organelles...
- GENE EXPRESSION OF THE YEAST PLASMID 2 MICRON CIRCLEJames Broach; Fiscal Year: 1991..We have purified the REP1 protein and shown that it is a component of the yeast nuclear matrix. Further, we have found that it undergoes calcium induced aggregation and binds specifically to discrete ..
- ROLE OF THE NUCLEAR MATRIX PROTEIN NRB/B IN BRAINShalom Avraham; Fiscal Year: 2002DESCRIPTION(Verbatim from Applicant's Abstract): The nuclear matrix is defined as the insoluble framework of the nucleus and has been implicated in modulating gene expression, the cell cycle and nuclear structural integrity via linkage ..
- NUCLEAR PROTEINS AND REGULATION OF LYMPHOCYTE GROWTHNili Feuerstein; Fiscal Year: 1993..Recently, we have shown that a nuclear protein which is tightly associated with the nuclear matrix (termed "numatrin") may have an important role in the regulation of cell growth in normal and in neoplastic ..
- NUCLEAR MATRIX STRUCTURES AND GENOMIC FUNCTIONRonald Berezney; Fiscal Year: 2003..This organization is strikingly maintained following extraction of cells for nuclear matrix under conditions which maintained the DNA intact...
- NUCLEAR MATRIX STRUCTURES AND GENOMIC FUNCTIONRonald Berezney; Fiscal Year: 2002..This organization is strikingly maintained following extraction of cells for nuclear matrix under conditions which maintained the DNA intact...
- Epigenetic Regulation in Self-Renewing and Differentiating Male Germ CellsChristopher Payne; Fiscal Year: 2009..The second aim will examine the role of nuclear matrix proteins in the maintenance of self-renewing spermatogonia...
- NEW NUCLEAR MATRIX PROTEIN IN BREAST CANCER GROWTHSteffi Oesterreich; Fiscal Year: 2002..she cloned a novel protein (HET) which apparently is identical to a recently cloned scaffold attachment factor/nuclear matrix protein...
- NUCLEAR MATRIX AND ACTIN--BIOMARKERS FOR BLADDER CANCERRobert Veltri; Fiscal Year: 1992Cytoskeletal and nuclear matrix proteins (NMPs) play an important role in the bio-chemical events involved in differentiation and transformation of mammalian cells...
- TRANSCRIPTIONAL REGULATION AND TUMORIGENESIS BY MMTVJAQUELIN DUDLEY; Fiscal Year: 2002..Two nuclear protein complexes bind to the NREs. Those complexes contain the nuclear matrix-associated proteins SATB1 and Cux/CDP...
- Nuclear Matrix Structure and Genomic FunctionRonald Berezney; Fiscal Year: 2007..The striking structural preservation of a variety of genomic processes on the nuclear matrix provides an important approach for correlating nuclear form and function...
- COLLAGEN IN OSTEOARTHRITIC CARTILAGEPAUL BENYA; Fiscal Year: 1991..modifications in microfilament structure are physically transmit:ed into the nucleus, by way of cytoskeletal-nuclear matrix interactions, and result in changes in gene expression, and the possibility that modification may generate ..
- TARGETING CELLULAR ONCOGENES WITH DNA-REACTIVE DRUGSJan Woynarowski; Fiscal Year: 2000..this application is to explore the possibility that AT-specific DNA-reactive drugs could preferentially target nuclear matrix-bound oncogene domains...
- GENETIC MECHANISMS THAT POTENTIATE HUMAN SPERMATOGENESISSTEPHEN KRAWETZ; Fiscal Year: 2009..As we and others have shown, a likely key player in this mechanism of transcriptional regulation is the nuclear matrix attachment region...
- NUCLEAR TARGETING OF SIGNAL TRANSDUCTION ENZYMESVINCENT COGHLAN; Fiscal Year: 2000..have discovered a novel anchoring protein, called AKAP 95, that binds PKA and is exclusively localized to the nuclear matrix. The properties of this AKAP will be characterized as part of a long term goal to define the relationship ..
- PURIFICATION OF A BONE NUCLEAR MATRIX PROTEINJOSEPH BIDWELL; Fiscal Year: 1999..These studies are designed to test the hypothesis that nuclear matrix protein 4 (NMP4) a novel, PTH-responsive osteoblast nuclear matrix factor, plays a role in promoting ..