nuclear matrix

Summary

Summary: The residual framework structure of the CELL NUCLEUS that maintains many of the overall architectural features of the cell nucleus including the nuclear lamina with NUCLEAR PORE complex structures, residual CELL NUCLEOLI and an extensive fibrogranular structure in the nuclear interior. (Advan. Enzyme Regul. 2002; 42:39-52)

Top Publications

  1. ncbi HA95 is a protein of the chromatin and nuclear matrix regulating nuclear envelope dynamics
    S B Martins
    Institute of Medical Biochemistry, University of Oslo, PO Box 1112 Blindern, Norway
    J Cell Sci 113:3703-13. 2000
  2. ncbi Internal organisation of the nucleus: assembly of compartments by macromolecular crowding and the nuclear matrix model
    Ronald Hancock
    Laval University Cancer Research Centre, Hotel Dieu Hospital, 9 rue MacMahon, Quebec, P Q G1R 2J6, Canada
    Biol Cell 96:595-601. 2004
  3. ncbi Nuclear matrix support of DNA replication
    Boyka Anachkova
    Institute of Molecular Biology, Bulgarian Academy of Sciences, Akad G Bonchev Street, Bl 21, Sofia 1113, Bulgaria
    J Cell Biochem 96:951-61. 2005
  4. pmc PIASy, a nuclear matrix-associated SUMO E3 ligase, represses LEF1 activity by sequestration into nuclear bodies
    S Sachdev
    Gene Center and Institute of Biochemistry, University of Munich, 81377 Munich, Germany
    Genes Dev 15:3088-103. 2001
  5. ncbi The sperm nuclear matrix is required for paternal DNA replication
    Jeffrey A Shaman
    Institute for Biogenesis Research, John A Burns School of Medicine, University of Hawaii, Honolulu, Hawaii 96822, USA
    J Cell Biochem 102:680-8. 2007
  6. pmc Human telomeres are attached to the nuclear matrix
    T de Lange
    Rockefeller University, New York, NY 10021
    EMBO J 11:717-24. 1992
  7. ncbi An intact sperm nuclear matrix may be necessary for the mouse paternal genome to participate in embryonic development
    W S Ward
    Division of Urology, Robert Wood Johnson Medical School, New Brunswick, New Jersey 08903, USA
    Biol Reprod 60:702-6. 1999
  8. pmc A novel DNA-binding motif in the nuclear matrix attachment DNA-binding protein SATB1
    K Nakagomi
    La Jolla Cancer Research Foundation, California 92037
    Mol Cell Biol 14:1852-60. 1994
  9. pmc A hyperphosphorylated form of the large subunit of RNA polymerase II is associated with splicing complexes and the nuclear matrix
    M J Mortillaro
    Department of Biological Sciences, State University of New York at Buffalo 14260, USA
    Proc Natl Acad Sci U S A 93:8253-7. 1996
  10. ncbi The role of primer recognition proteins in DNA replication: association with nuclear matrix in HeLa cells
    J K Vishwanatha
    Department of Biochemistry, University of Nebraska Medical Center, Omaha 68198 4525
    J Cell Sci 101:25-34. 1992

Detail Information

Publications315 found, 100 shown here

  1. ncbi HA95 is a protein of the chromatin and nuclear matrix regulating nuclear envelope dynamics
    S B Martins
    Institute of Medical Biochemistry, University of Oslo, PO Box 1112 Blindern, Norway
    J Cell Sci 113:3703-13. 2000
    ..Biochemical and photobleaching data indicate that HA95 is tightly associated with chromatin and the nuclear matrix/lamina network in interphase, and bound to chromatin at mitosis...
  2. ncbi Internal organisation of the nucleus: assembly of compartments by macromolecular crowding and the nuclear matrix model
    Ronald Hancock
    Laval University Cancer Research Centre, Hotel Dieu Hospital, 9 rue MacMahon, Quebec, P Q G1R 2J6, Canada
    Biol Cell 96:595-601. 2004
    ..nucleus are segregated into discrete compartments, but the current model that this is achieved by a fibrillar nuclear matrix which structures the nuclear interior and compartments is not consistent with all experimental observations, ..
  3. ncbi Nuclear matrix support of DNA replication
    Boyka Anachkova
    Institute of Molecular Biology, Bulgarian Academy of Sciences, Akad G Bonchev Street, Bl 21, Sofia 1113, Bulgaria
    J Cell Biochem 96:951-61. 2005
    ..To achieve this, DNA is organized into loops attached to the nuclear matrix. Each loop represents one individual replicon with the origin of replication localized within the loop and the ..
  4. pmc PIASy, a nuclear matrix-associated SUMO E3 ligase, represses LEF1 activity by sequestration into nuclear bodies
    S Sachdev
    Gene Center and Institute of Biochemistry, University of Munich, 81377 Munich, Germany
    Genes Dev 15:3088-103. 2001
    ..Moreover, PIASy binds to nuclear matrix-associated DNA sequences and targets LEF1 to nuclear bodies, suggesting that PIASy-mediated subnuclear ..
  5. ncbi The sperm nuclear matrix is required for paternal DNA replication
    Jeffrey A Shaman
    Institute for Biogenesis Research, John A Burns School of Medicine, University of Hawaii, Honolulu, Hawaii 96822, USA
    J Cell Biochem 102:680-8. 2007
    ..We previously demonstrated that mammalian sperm nuclei contain a nuclear matrix that organizes the DNA into loop domains in a manner similar to that of somatic cells...
  6. pmc Human telomeres are attached to the nuclear matrix
    T de Lange
    Rockefeller University, New York, NY 10021
    EMBO J 11:717-24. 1992
    This report shows that human telomeres are tightly associated with the nuclear matrix. Telomere attachment is observed in several cell types and in all stages of interphase...
  7. ncbi An intact sperm nuclear matrix may be necessary for the mouse paternal genome to participate in embryonic development
    W S Ward
    Division of Urology, Robert Wood Johnson Medical School, New Brunswick, New Jersey 08903, USA
    Biol Reprod 60:702-6. 1999
    ..We also tested the stability of the sperm nuclear matrix by the ability to form nuclear halos. Sperm nuclei washed in freshly prepared 0...
  8. pmc A novel DNA-binding motif in the nuclear matrix attachment DNA-binding protein SATB1
    K Nakagomi
    La Jolla Cancer Research Foundation, California 92037
    Mol Cell Biol 14:1852-60. 1994
    The nuclear matrix attachment DNA (MAR) binding protein SATB1 is a sequence context-specific binding protein that binds in the minor groove, making virtually no contact with the DNA bases...
  9. pmc A hyperphosphorylated form of the large subunit of RNA polymerase II is associated with splicing complexes and the nuclear matrix
    M J Mortillaro
    Department of Biological Sciences, State University of New York at Buffalo 14260, USA
    Proc Natl Acad Sci U S A 93:8253-7. 1996
    ..retained in a similar pattern following in situ extraction of cells and was quantitatively recovered in the nuclear matrix fraction...
  10. ncbi The role of primer recognition proteins in DNA replication: association with nuclear matrix in HeLa cells
    J K Vishwanatha
    Department of Biochemistry, University of Nebraska Medical Center, Omaha 68198 4525
    J Cell Sci 101:25-34. 1992
    ..we have investigated the subcellular distribution and specific association of these proteins with the nuclear matrix in HeLa cells...
  11. ncbi A proteomic study of the arabidopsis nuclear matrix
    Tomasz T Calikowski
    Department of Plant Biology and Plant Biotechnology Center, Ohio State University, Columbus, Ohio 43210, USA
    J Cell Biochem 90:361-78. 2003
    ..to be organized by two interdependent nucleoprotein structures, the DNA-based chromatin and the RNA-dependent nuclear matrix. The functional composition and molecular organization of the second component have not yet been resolved...
  12. ncbi Functional domains of necdin for protein-protein interaction, nuclear matrix targeting, and cell growth suppression
    Hideo Taniura
    Graduate School of Natural Science and Technology, Kanazawa University, Kakumamachi, Kanazawa, Ishikawa 920 1192, Japan
    J Cell Biochem 94:804-15. 2005
    ..We here characterize the regions of necdin required for the protein-protein interaction, nuclear matrix targeting, and cell growth suppression...
  13. ncbi Dynamics of nuclear matrix proteome during embryonic development in Drosophila melanogaster
    Parul Varma
    Center for Cellular and Molecular Biology CSIR, Hyderabad 500 007, India
    J Biosci 36:439-59. 2011
    ..of nuclear architecture, which is believed to play an important role in regulation of gene expression, is the nuclear matrix (NuMat)...
  14. ncbi Lamin proteins form an internal nucleoskeleton as well as a peripheral lamina in human cells
    P Hozak
    CRC Nuclear Structure and Function Research Group, Sir William Dunn School of Pathology, University of Oxford, UK
    J Cell Sci 108:635-44. 1995
    ..Knobs and nodes on an internal skeleton can then be immunolabelled using fluorescein- or gold-conjugated anti-lamin A antibodies. These results suggest that the lamins are misnamed as they are also found internally...
  15. ncbi Necdin interacts with the ribonucleoprotein hnRNP U in the nuclear matrix
    Hideo Taniura
    Division of Regulation of Macromolecular Functions, Institute for Protein Research, Osaka University, Yamadaoka 3 2, Suita, Osaka 565 0871, Japan
    J Cell Biochem 84:545-55. 2002
    ..contained cDNA encoding a carboxyl-terminal portion of heterogeneous nuclear ribonucleoprotein U (hnRNP U), a nuclear matrix-associated protein that interacts with chromosomal DNA...
  16. pmc A novel form of the telomere-associated protein TIN2 localizes to the nuclear matrix
    Patrick G Kaminker
    Buck Institute for Age Research, Novato, California, USA
    Cell Cycle 8:931-9. 2009
    ..TIN2S) in two ways: TIN2L contains an additional 97 amino acids, and TIN2L associates strongly with the nuclear matrix. Stringent salt and detergent conditions failed to extract TIN2L from the nuclear matrix, despite removing ..
  17. pmc PDLIM2 suppresses human T-cell leukemia virus type I Tax-mediated tumorigenesis by targeting Tax into the nuclear matrix for proteasomal degradation
    Pengrong Yan
    University of Pittsburgh Cancer Institute, University of Pittsburgh Medical Center, PA, USA
    Blood 113:4370-80. 2009
    ..In addition, PDLIM2 recruited Tax from its functional sites into the nuclear matrix where the polyubiquitinated Tax was degraded by the proteasome...
  18. ncbi From DNA structure to gene expression: mediators of nuclear compartmentalization and dynamics
    J Bode
    GBF German Research Center for Biotechnology Epigenetic Regulation, Mascheroder Weg 1, D 38124 Braunschweig
    Chromosome Res 11:435-45. 2003
    ..into chromatin domains by their attachment to a supporting structure that has traditionally been termed the nuclear matrix. Present evidence indicates the dynamics of this entity, which requires particular properties of the elements ..
  19. pmc CENP-F is a protein of the nuclear matrix that assembles onto kinetochores at late G2 and is rapidly degraded after mitosis
    H Liao
    Fox Chase Cancer Center, Institute for Cancer Research, Philadelphia, Pennsylvania 19111, USA
    J Cell Biol 130:507-18. 1995
    ..CENP-F is protein of the nuclear matrix that gradually accumulates during the cell cycle until it reaches peak levels in G2 and M phase cells and is ..
  20. pmc Anchoring the genome
    Diego Ottaviani
    Cancer Research UK London Research Institute, Lincoln s Inn Fields, London WC2A 3PX, UK
    Genome Biol 9:201. 2008
    ..is evidence that higher-order chromatin folding is mediated by the anchoring of specific DNA sequences to the nuclear matrix. These genome anchors are also crucial regulators of gene expression and DNA replication, and play a role in ..
  21. pmc Dynamics of association of origins of DNA replication with the nuclear matrix during the cell cycle
    V Djeliova
    Institute of Molecular Biology, Bulgarian Academy of Sciences, Akad. G. Bonchev Street, Bl. 21, Sofia 1113, Bulgaria
    Nucleic Acids Res 29:3181-7. 2001
    DNA of replication foci attached to the nuclear matrix was isolated from Chinese hamster ovary cells and human HeLa cells synchronized at different stages of the G(1) and S phases of the cell cycle...
  22. ncbi Fanconi anemia proteins localize to chromatin and the nuclear matrix in a DNA damage- and cell cycle-regulated manner
    F Qiao
    Departments of Microbiology and Pediatrics, University of Virginia and the University of Virginia Health System, Charlottesville, Virginia 22908, USA
    J Biol Chem 276:23391-6. 2001
    ..Biochemical fractionation reveals that the FA proteins are found in nuclear matrix and chromatin and that treatment with mitomycin C results in increase of the FA proteins in nuclear matrix and ..
  23. pmc Relationship between DNA replication and the nuclear matrix
    Rosemary H C Wilson
    Department of Biology, University of York, Heslington, York, YO10 5DD, UK
    Genes Cells 18:17-31. 2013
    There is an extensive list of primary published work related to the nuclear matrix (NM)...
  24. ncbi The fate of the nuclear matrix-associated-region-binding protein SATB1 during apoptosis
    J Gotzmann
    Institute of Tumor Biology Cancer Research, University of Vienna, A 1090 Vienna, Austria
    Cell Death Differ 7:425-38. 2000
    ..protein 1 (SATB1), predominantly expressed in thymocytes, was identified as a component of the nuclear matrix protein fraction...
  25. ncbi Minicircle performance depending on S/MAR-nuclear matrix interactions
    Sandra Broll
    Department Molecular Biotechnology Epigenetic Regulation, Helmholtz Center for Infection Research, Inhoffenstrasse 7, D 38124 Braunschweig, Germany
    J Mol Biol 395:950-65. 2010
    ....
  26. ncbi Identification of a candidate regulatory region in the human CD8 gene complex by colocalization of DNase I hypersensitive sites and matrix attachment regions which bind SATB1 and GATA-3
    Lynda J Kieffer
    Department of Laboratory Medicine and Department of Genetics and Section of Immunobiology, Yale University, New Haven, CT 06520, USA
    J Immunol 168:3915-22. 2002
    To locate elements regulating the human CD8 gene complex, we mapped nuclear matrix attachment regions (MARs) and DNase I hypersensitive (HS) sites over a 100-kb region that included the CD8B gene, the intergenic region, and the CD8A gene...
  27. ncbi Nuclear matrix bound fibroblast growth factor receptor is associated with splicing factor rich and transcriptionally active nuclear speckles
    Suryanarayan Somanathan
    Department of Biological Sciences, State University of New York at Buffalo, Buffalo, New York 14260, USA
    J Cell Biochem 90:856-69. 2003
    ..arrangements of FGFR1 sites and colocalization with nuclear speckles were maintained following extraction for nuclear matrix. Moreover, immunoblots indicated a significant enrichment of FGFR1 in the nuclear matrix fraction...
  28. ncbi The PML gene encodes a phosphoprotein associated with the nuclear matrix
    K S Chang
    Department of Hematology, University of Texas M D Anderson Cancer Center, Houston 77030, USA
    Blood 85:3646-53. 1995
    ..In K562 and NIH/3T3 cells transfected with a PML expression plasmid, we found PML to be associated with the nuclear matrix. Our results also showed that PML is a phosphorprotein...
  29. ncbi The sperm nucleus: chromatin, RNA, and the nuclear matrix
    Graham D Johnson
    The Center for Molecular Medicine and Genetics, C S Mott Center, Wayne State University of Medicine, 275 East Hancock, Detroit, MI 48201, USA
    Reproduction 141:21-36. 2011
    ..These regions may be necessary for organizing higher order genomic structure through interactions with the nuclear matrix. The promoters of this transcriptionally quiescent genome are differentially marked by modified histones that ..
  30. pmc Cyclin E is recruited to the nuclear matrix during differentiation, but is not recruited in cancer cells
    Jennifer Munkley
    Department of Biology, University of York, YO10 5YW and School of Medicine, Leeds University, LS2 9JT, UK
    Nucleic Acids Res 39:2671-7. 2011
    ..We show that cyclin E, but not A, is mounted upon the nuclear matrix in sub-nuclear foci in differentiated vertebrate cells, but not in undifferentiated cells or cancer cells...
  31. ncbi Proto-oncoprotein TLS/FUS is associated to the nuclear matrix and complexed with splicing factors PTB, SRm160, and SR proteins
    Michael Meissner
    Institute of Cancer Research, Borschkegasse 8a, University of Vienna, A 1090 Vienna, Austria
    Exp Cell Res 283:184-95. 2003
    ..Here we report the association of TLS/FUS with the nuclear matrix and investigate its role in splicing...
  32. ncbi Accumulation of LANA at nuclear matrix fraction is important for Kaposi's sarcoma-associated herpesvirus replication in latency
    Eriko Ohsaki
    Department of Infectious Diseases, University of Hamamatsu School of Medicine, 1 20 1 Handayama, Higashi ku, Hamamatsu, Shizuoka 431 3192, Japan
    Virus Res 139:74-84. 2009
    ..the KSHV genome, and a viral replication factor, latency-associated nuclear antigen (LANA) accumulate at the nuclear matrix fraction in the G1 phase...
  33. pmc S/MARt DB: a database on scaffold/matrix attached regions
    Ines Liebich
    Research Group Bioinformatics, GBF, Mascheroder Weg 1, D 38124 Braunschweig, Germany
    Nucleic Acids Res 30:372-4. 2002
    ..S/MAR transaction database, is a relational database covering scaffold/matrix attached regions (S/MARs) and nuclear matrix proteins that are involved in the chromosomal attachment to the nuclear scaffold...
  34. pmc Structural insights into SUN-KASH complexes across the nuclear envelope
    Wenjia Wang
    State Key Laboratory of Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China
    Cell Res 22:1440-52. 2012
    ..These results provide a structural model of the LINC complex, which is essential for additional study of the physical and functional coupling between the cytoplasm and the nucleoplasm...
  35. ncbi Function of the sperm nuclear matrix
    Jeffrey A Shaman
    Institute for Biogenesis Research, John A Burns School of Medicine, University of Hawaii Honolulu, HI 96822, USA
    Arch Androl 53:135-40. 2007
    ..One of these is the organization of DNA into loop domains attached at their bases to a proteinaceous nuclear matrix. Several groups have shown that the sites at which DNA associates with the sperm nuclear matrix contain ..
  36. ncbi Localization of prohibitin in the nuclear matrix and alteration of its expression during differentiation of human neuroblastoma SK-N-SH cells induced by retinoic acid
    Qi fu Li
    The Key Laboratory of Ministry of Education for Cell Biology and Tumor Cell Engineering, School of Life Science, Xiamen University, China
    Cell Mol Neurobiol 31:203-11. 2011
    The nuclear matrix-intermediate filament system of human neuroblastoma SK-N-SH cells before and after retinoic acid (RA) treatment was selectively extracted and the distribution of prohibitin (PHB) in the nuclear matrix, as well as its ..
  37. ncbi Exploring the relationship between interphase gene positioning, transcriptional regulation and the nuclear matrix
    Lauren S Elcock
    Laboratory of Nuclear and Genomic Health, Centre for Cell and Chromosome Biology, Brunel University, Uxbridge UB8 3PH, UK
    Biochem Soc Trans 38:263-7. 2010
    ..at a more local scale? During the course of this review, these questions will be considered, in light of the current literature regarding the role of transcription factories and the nuclear matrix in interphase genome organization.
  38. ncbi DNA damage-dependent interaction of the nuclear matrix protein C1D with Translin-associated factor X (TRAX)
    Tuba Erdemir
    Bilkent University, Molecular Biology and Genetics Department, 06533, Bilkent, Ankara, Turkey
    J Cell Sci 115:207-16. 2002
    The nuclear matrix protein C1D is an activator of the DNA-dependent protein kinase (DNA-PK), which is essential for the repair of DNA double-strand breaks (DSBs) and V(D)J recombination...
  39. ncbi Aged and post-mitotic cells share a very stable higher-order structure in the cell nucleus in vivo
    Janeth Alva-Medina
    Universidad Autonoma del Estado de Mexico, Toluca, Estado de Mexico, Mexico
    Biogerontology 11:703-16. 2010
    ..cells the nuclear DNA is organized in supercoiled loops anchored to a proteinaceous substructure known as the nuclear matrix (NM). The DNA-NM interactions define a higher-order structure in the cell nucleus (NHOS)...
  40. ncbi Hcc-1 is a novel component of the nuclear matrix with growth inhibitory function
    C L Leaw
    Bioprocessing Technology Institute, Agency for Science, Technology and Research A STAR, Singapore
    Cell Mol Life Sci 61:2264-73. 2004
    ..0173) and an accumulation of cells at the G2/M phase (p = 0.0276 compared to control HEK293 cells). Taken together, these results suggest a role for Hcc-1 in growth regulation and nucleic acids metabolism...
  41. ncbi A new spectrin, beta IV, has a major truncated isoform that associates with promyelocytic leukemia protein nuclear bodies and the nuclear matrix
    W T Tse
    Division of Hematology Oncology, Children s Hospital, and the Dana Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 276:23974-85. 2001
    ..Spectrin betaIV is the first beta-spectrin associated with a subnuclear structure and may be part of a nuclear scaffold to which gene regulatory machinery binds...
  42. pmc A structural basis for cellular senescence
    Armando Aranda-Anzaldo
    Laboratorio de Biologia Molecular, Facultad de Medicina, Universidad Autonoma del Estado de Mexico, Paseo Tollocan y Jesús Carranza, Toluca, Edo Mex, Mexico
    Aging (Albany NY) 1:598-607. 2009
    ..and discuss evidence that the interactions between DNA and the nuclear substructure, commonly known as the nuclear matrix, define a higher-order structure within the cell nucleus that following thermodynamic constraints, ..
  43. ncbi Order and disorder in the nucleus
    Wallace F Marshall
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, Connecticut 06520, USA
    Curr Biol 12:R185-92. 2002
    ....
  44. ncbi NeuN/Fox-3 is an intrinsic component of the neuronal nuclear matrix
    Myrna A R Dent
    Facultad de Medicina, Universidad Autonoma del Estado de Mexico, Toluca, Mexico, Mexico
    FEBS Lett 584:2767-71. 2010
    ..Here we provide evidence that NeuN/Fox-3 is an intrinsic component of the neuronal nuclear matrix and a reliable marker of nuclear speckles in neurons.
  45. ncbi Nuclear structure in cancer cells
    Daniele Zink
    University of Munich LMU, Department of Biology II, Goethestr 31, 80336 Munich, Germany
    Nat Rev Cancer 4:677-87. 2004
    ..Advances in understanding nuclear structure have revealed insights into the process of malignant transformation and provide a basis for the development of new diagnostic tools and therapeutics...
  46. pmc Silencing by nuclear matrix attachment distinguishes cell-type specificity: association with increased proliferation capacity
    Amelia K Linnemann
    Department of Obstetrics and Gynecology, The Center for Molecular Medicine and Genetics, Wayne State University School of Medicine, Detroit, MI 48201, USA
    Nucleic Acids Res 37:2779-88. 2009
    ..We have previously shown that attachment of genes to the NaCl-isolated nuclear matrix correlates with their silencing in HeLa cells...
  47. ncbi The cerebellar leucine-rich acidic nuclear protein interacts with ataxin-1
    A Matilla
    Department of Pediatrics, Baylor College of Medicine, Houston, Texas 77030, USA
    Nature 389:974-8. 1997
    ..Immunofluorescence studies demonstrate that both LANP and ataxin-1 colocalize in nuclear matrix-associated subnuclear structures...
  48. ncbi CpG-binding protein is a nuclear matrix- and euchromatin-associated protein localized to nuclear speckles containing human trithorax. Identification of nuclear matrix targeting signals
    Jeong Heon Lee
    Herman B Wells Center for Pediatric Research, Section of Pediatric Hematology Oncology, Department of Pediatrics and Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis 46202, USA
    J Biol Chem 277:42259-67. 2002
    ..CGBP associates with the nuclear matrix, and fragments of CGBP that fail to associate with the nuclear matrix fail to localize to nuclear speckles and ..
  49. ncbi Experimental observations of a nuclear matrix
    J Nickerson
    Department of Cell Biology and Cancer Center, University of Massachusetts Medical School, Worcester, MA 01655, USA
    J Cell Sci 114:463-74. 2001
    ..architecture of the nucleus includes two overlapping and nucleic-acid-containing structures - chromatin and a nuclear matrix. The nuclear matrix is observed by microscopy in live, fixed and extracted cells...
  50. pmc The amino-terminal region of the retinoblastoma gene product binds a novel nuclear matrix protein that co-localizes to centers for RNA processing
    T Durfee
    Center for Molecular Medicine Institute of Biotechnology, University of Texas Health Science Center at San Antonio 78245
    J Cell Biol 127:609-22. 1994
    ..The only gene isolated from this screen encodes a novel 84-kD nuclear matrix protein that localizes to subnuclear regions associated with RNA processing...
  51. ncbi Ataxin-3 is transported into the nucleus and associates with the nuclear matrix
    D Tait
    Max Planck Institut fur Molekulare Genetik, Ihnestrasse 73, D 14195 Berlin Dahlem, Germany
    Hum Mol Genet 7:991-7. 1998
    ..revealed that the ataxin-3 protein present in the nucleus of neuroblastoma cells is associated with the inner nuclear matrix. Our results taken together with the finding of a nuclear localization signal in ataxin-3 indicate that the ..
  52. ncbi Nuclear matrix interactions within the sperm genome
    J A Kramer
    Department of Obstetrics and Gynecology, Center for Molecular Medicine and Genetics, Wayne State University School of Medicine, Detroit, Michigan 48102, USA
    J Biol Chem 271:11619-22. 1996
    ..human PRM1 --> PRM2 --> TNP2 genic domain has revealed two regions of attachment to the sperm nuclear matrix. These sperm nuclear matrix attachment regions delimit the DNase I-sensitive domain of this haploid-expressed ..
  53. ncbi A new look at the nuclear matrix
    R Hancock
    Centre de recherche en cancérologie de l Université Laval, Hotel Dieu Hospital, Quebec, Canada
    Chromosoma 109:219-25. 2000
    The concept of the nuclear matrix, a karyoskeletal structure that serves as a support for the genome and its activities, has stimulated many studies of the association of nuclear components and functions with this structure...
  54. ncbi Cloning and expression of a novel nuclear matrix-associated protein that is regulated during the retinoic acid-induced neuronal differentiation
    W M Cheung
    Department of Biochemistry, Biotechnology Research Institute, and Molecular Neuroscience Center, Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong, China
    J Biol Chem 276:17083-91. 2001
    ..primed polymerase chain reaction, we identified a novel serine/threonine-rich protein, RA-regulated nuclear matrix-associated protein (Ramp), that was down-regulated during the RA-induced differentiation of NT2 cells...
  55. pmc Characterization of SAF-A, a novel nuclear DNA binding protein from HeLa cells with high affinity for nuclear matrix/scaffold attachment DNA elements
    H Romig
    Department of Biology, University of Konstanz, Germany
    EMBO J 11:3431-40. 1992
    ..SAF-A is an abundant nuclear protein and a constituent of the nuclear matrix and scaffold...
  56. ncbi Interaction of EBV latent origin of replication with the nuclear matrix: identification of S/MAR sequences and protein components
    Giulia Mearini
    Department of Public Health Sciences, University La Sapienza, Rome, Italy
    FEBS Lett 547:119-24. 2003
    During latency, Epstein Barr virus (EBV) genome, as an episome, is attached to the nuclear matrix (NM) via the latent origin of replication ori P...
  57. pmc DNA moves sequentially towards the nuclear matrix during DNA replication in vivo
    Juan Carlos Rivera-Mulia
    Laboratorio de Biologia Molecular, Facultad de Medicina, Universidad Autonoma del Estado de Mexico, Apartado Postal 428, CP 50000 Toluca, Edo Mex, Mexico
    BMC Cell Biol 12:3. 2011
    In the interphase nucleus of metazoan cells DNA is organized in supercoiled loops anchored to a nuclear matrix (NM)...
  58. ncbi Unraveling the organization of the internal nuclear matrix: RNA-dependent anchoring of NuMA to a lamin scaffold
    Paola Barboro
    Istituto Nazionale per la Ricerca sul Cancro, I 16132, Genova, Italy
    Exp Cell Res 279:202-18. 2002
    Using quantitative immunoelectron microscopy we show here that when the nuclear matrix is isolated from rat hepatocytes in the presence of an inhibitor of RNase activity both lamins and the nuclear mitotic apparatus protein (NuMA) ..
  59. pmc Mapping of the nuclear matrix-bound chromatin hubs by a new M3C experimental procedure
    Alexey A Gavrilov
    Laboratory of Structural and Functional Organization of Chromosomes, Institute of Gene Biology of the Russian Academy of Sciences, 34 5 Vavilov Street, 119334 Moscow, Russia
    Nucleic Acids Res 38:8051-60. 2010
    We have developed an experimental procedure to analyze the spatial proximity of nuclear matrix-bound DNA fragments...
  60. pmc Sp2 localizes to subnuclear foci associated with the nuclear matrix
    K Scott Moorefield
    Graduate Program in Genomic Sciences, Center for Comparative Medicine and Translational Research, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27606, USA
    Mol Biol Cell 17:1711-22. 2006
    ..We report that 1) Sp2 localizes largely within subnuclear foci associated with the nuclear matrix, and 2) these foci are distinct from promyelocytic oncogenic domains and appear to be stable during an 18-h ..
  61. ncbi Nuclear matrix association of insulin receptor and IRS-1 by insulin in osteoblast-like UMR-106 cells
    Ki Cheon Seol
    Department of Pharmacology, School of Dentistry, Kyung Hee University, Seoul 130 701, Republic of Korea
    Biochem Biophys Res Commun 306:898-904. 2003
    ..The majority of insulin receptor and IRS-1 translocated to the nucleus appeared to associate with nuclear matrix. Tyrosine phosphorylation of a number of proteins with a molecular mass of 180, 95, 85, or 70 kDa in the ..
  62. ncbi The newly identified human nuclear protein NXP-2 possesses three distinct domains, the nuclear matrix-binding, RNA-binding, and coiled-coil domains
    Yukio Kimura
    Department of Life Science, Graduate School and Faculty of Science, Himeji Institute of Technology, Kamigori, Hyogo 678 1201, Japan
    J Biol Chem 277:20611-7. 2002
    Using a monoclonal antibody that recognizes a nuclear matrix protein, we selected a cDNA clone from a lambdagt11 human placenta cDNA library. This cDNA encoded a 939-amino acid protein designated nuclear matrix protein NXP-2...
  63. ncbi c-Abl tyrosine kinase selectively regulates p73 nuclear matrix association
    Merav Ben-Yehoyada
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
    J Biol Chem 278:34475-82. 2003
    ..that in response to ionizing radiation, p73 undergoes nuclear redistribution and becomes associated with the nuclear matrix. This association is c-Abl-dependent because it was not observed in cells that are defective in c-Abl kinase ..
  64. ncbi Interactions of Epstein-Barr virus origins of replication with nuclear matrix in the latent and in the lytic phases of viral infection
    E Mattia
    Faculty of Medicine and Surgery, University of Roma La Sapienza, Rome, Italy
    Virology 262:9-17. 1999
    Eukaryotic DNA is organized into domains or loops generated by the attachment of chromatin fibers to the nuclear matrix via specific regions called scaffold or matrix attachment regions...
  65. pmc Gene positional changes relative to the nuclear substructure correlate with the proliferating status of hepatocytes during liver regeneration
    Apolinar Maya-Mendoza
    Laboratorio de Biologia Molecular, Facultad de Medicina, Universidad Autonoma del Estado de Mexico, Apartado Postal 428, C P 50000, Toluca, Edo Mex, Mexico
    Nucleic Acids Res 31:6168-79. 2003
    ..is organised in loops anchored to a proteinaceous substructure variously named but commonly known as the nuclear matrix. Important processes of nuclear physiology, such as replication, transcription and processing of primary ..
  66. pmc Autosomal-dominant distal myopathy associated with a recurrent missense mutation in the gene encoding the nuclear matrix protein, matrin 3
    Jan Senderek
    Institute of Cell Biology, ETH Zurich, 8093 Zurich, Switzerland
    Am J Hum Genet 84:511-8. 2009
    ..MATR3 is expressed in skeletal muscle and encodes matrin 3, a component of the nuclear matrix, which is a proteinaceous network that extends throughout the nucleus...
  67. ncbi Re-defining the chromatin loop domain
    H H Heng
    Center for Molecular Medicine and Genetics, Department of Pathology, Karmanos Cancer Institute, Wayne State University School of Medicine, Detroit MI 48202, USA
    Cytogenet Cell Genet 93:155-61. 2001
    ..to target some long-standing issues regarding the structure and function of the chromatin loop domain and its relationship with the nuclear matrix. This new knowledge will have a profound impact for modern genetics and molecular medicine.
  68. pmc Boundary element-associated factor 32B connects chromatin domains to the nuclear matrix
    Rashmi U Pathak
    Department of Biochemistry, School of Life Sciences, University of Hyderabad, Hyderabad, India
    Mol Cell Biol 27:4796-806. 2007
    ..In this report we show that BEAF is associated with the nuclear matrix and map the domain required for matrix association to the middle region of the protein...
  69. ncbi Gene positional changes relative to the nuclear substructure during carbon tetrachloride-induced hepatic fibrosis in rats
    Apolinar Maya-Mendoza
    Laboratorio de Biologia Molecular, Facultad de Medicina, Universidad Autonoma del Estado de Mexico, Apdo Postal 428, C P 50000, Toluca, Edo Mex, Mexico
    J Cell Biochem 93:1084-98. 2004
    ..interphase nucleus the DNA of higher eukaryotes is organized in loops anchored to a substructure known as the nuclear matrix (NM)...
  70. ncbi Exploring the effects of a dysfunctional nuclear matrix
    Lauren S Elcock
    Laboratory of Nuclear and Genomic Health, Centre for Cell and Chromosome Biology, Brunel University, Uxbridge, UK
    Biochem Soc Trans 36:1378-83. 2008
    The nuclear matrix has remained a contentious structure for decades; many believe that it is an artefact of harsh non-physiological procedures...
  71. ncbi Initiation of DNA replication at a nuclear matrix-attached chromatin fraction
    Ilian Radichev
    Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia, Bulgaria
    J Cell Physiol 203:71-7. 2005
    ..To address this issue, we developed a cell-free replication system in which the nuclear matrix along with the residual matrix-attached chromatin was used as a substrate for DNA replication...
  72. pmc Differential nuclear scaffold/matrix attachment marks expressed genes
    Amelia K Linnemann
    The Center for Molecular Medicine and Genetics, Wayne State University School of Medicine, C S Mott Center, Detroit, MI48201, USA
    Hum Mol Genet 18:645-54. 2009
    ..The varied functions of the S/MARs as revealed by the different extraction methods highlights their unique functional contribution...
  73. ncbi Localization and altered expression of nucleophosmin in the nuclear matrix during the differentiation of human hepatocarcinoma SMMC-7721 cells induced by HMBA
    Qi fu Li
    The Key Laboratory of Ministry of Education for Cell Biology and Tumor Cell Engineering, School of Life Sciences, Xiamen University, Xiamen, PR China
    Cancer Invest 28:1004-12. 2010
    ..To investigate the mechanism of induced differentiation of tumor cells, the nuclear matrix of human hepatocarcinoma SMMC-7721 cells induced by hexamethylene bisacetamide (HMBA) was selectively ..
  74. ncbi Age-related association of rDNA and telomeres with the nuclear matrix in mouse hepatocytes
    Alberto S Moraes
    Department of Cell Biology, Institute of Biology, State University of Campinas, 13083 863 Campinas, Sao Paulo, Brazil
    Cell Biol Int 34:925-31. 2010
    Transcribed sequences have been suggested to be associated with the nuclear matrix, differing from non-transcribing sequences, which have been reported to be contained in DNA loops...
  75. ncbi Characterization of a novel Dp71 dystrophin-associated protein complex (DAPC) present in the nucleus of HeLa cells: members of the nuclear DAPC associate with the nuclear matrix
    Lizeth Fuentes-Mera
    Department of Genetics and Molecular Biology, Centro de Investigacion y de Estudios Avanzados del IPN, Avenida Instituto Politecnico Nacional 2508, Apartado Postal 14 740, C P 07000, Mexico D F, Mexico
    Exp Cell Res 312:3023-35. 2006
    ..Next, we analyze the possible association of the nuclear DAPC with the nuclear matrix. We found the presence of Dp71, beta-dystroglycan, nNOS, beta-sarcoglycan, alpha/beta syntrophin, alpha1-..
  76. ncbi A-kinase-anchoring protein AKAP95 is targeted to the nuclear matrix and associates with p68 RNA helicase
    L Akileswaran
    Neurological Sciences Institute, Oregon Health Sciences University, Beaverton, Oregon 97006, USA
    J Biol Chem 276:17448-54. 2001
    The cell nucleus is structurally and functionally organized by the nuclear matrix. We have examined whether the nuclear cAMP-dependent protein kinase-anchoring protein AKAP95 contains specific signals for targeting to the subnuclear ..
  77. pmc Sequences attaching loops of nuclear and mitochondrial DNA to underlying structures in human cells: the role of transcription units
    D A Jackson
    CRC Nuclear Structure and Function Research Group, Sir William Dunn School of Pathology, University of Oxford, UK
    Nucleic Acids Res 24:1212-9. 1996
    ..These results are consistent with transcription being responsible for ever-changing attachments in both nuclei and mitochondria...
  78. ncbi Interaction of the nuclear matrix protein NAKAP with HypA and huntingtin: implications for nuclear toxicity in Huntington's disease pathogenesis
    Jonathan A Sayer
    Neurological Sciences Institute, Oregon Health and Science University, Beaverton, OR 97006, USA
    Neuromolecular Med 7:297-310. 2005
    ..In cultured cells, NAKAP and HypA localize within the nucleus and copurify with the nuclear matrix. Furthermore, NAKAP associates with HypA from human brain and copurifies with huntingtin protein in brain ..
  79. ncbi Emp is a component of the nuclear matrix of mammalian cells and undergoes dynamic rearrangements during cell division
    Shashi Bala
    Center of Cell Biology, Department of Medicine, Caritas St Elizabeth s Medical Center, Tufts University School of Medicine, Boston, MA 02135, USA
    Biochem Biophys Res Commun 342:1040-8. 2006
    ..Sequential extractions of interphase nuclei showed that recombinant Emp was present predominantly in the nuclear matrix. Immunofluorescence microscopy showed that Emp was present in typical nuclear speckles enriched with the ..
  80. ncbi Nuclear matrix protein SMAR1 represses HIV-1 LTR mediated transcription through chromatin remodeling
    Kadreppa Sreenath
    National Centre for Cell Science, University of Pune Campus, Ganeshkhind, Pune 411007, Maharashtra, India
    Virology 400:76-85. 2010
    b>Nuclear Matrix and MARs have been implicated in the transcriptional regulation of host as well as viral genes but their precise role in HIV-1 transcription remains unclear...
  81. pmc Nuclear matrix proteome analysis of Drosophila melanogaster
    Satish Kallappagoudar
    Centre for Cellular and Molecular Biology, Council of Scientific and Industrial Research, Hyderabad, India
    Mol Cell Proteomics 9:2005-18. 2010
    ..is unknown, a major component of this organization is likely to be the non-chromatin scaffolding called nuclear matrix (NuMat)...
  82. ncbi Drosophila dSAP18 is a nuclear protein that associates with chromosomes and the nuclear matrix, and interacts with pinin, a protein factor involved in RNA splicing
    Elisabet Costa
    Departament de Biologia Molecular i Cellular, Institut de Biologia Molecular de Barcelona IBMB CSIC, Parc Cientific de Barcelona, Josep Samitier 1 5, 08028, Barcelona, Spain
    Chromosome Res 14:515-26. 2006
    ..that, in Drosophila, dSAP18 is a predominantly nuclear protein that associates to both chromosomes and the nuclear matrix. dSAP18 becomes nuclear early during development, at the onset of cellularization, and remains so all through ..
  83. pmc Matrix attachment regions and structural colinearity in the genomes of two grass species
    Z Avramova
    Department of Biological Sciences and Purdue Genetics Program, Purdue University, West Lafayette, IN 47907, USA
    Nucleic Acids Res 26:761-7. 1998
    ....
  84. ncbi TRiC-P5, a novel TCP1-related protein, is localized in the cytoplasm and in the nuclear matrix
    E C Joly
    Institut du Cancer de Montreal, Centre de Recherche Louis Charles Simard, Quebec, Canada
    J Cell Sci 107:2851-9. 1994
    ..In addition, a significant part of TRiC-P5 is also found in the nucleus where it is attached to the nuclear matrix, a complex filament network involved in essential cellular functions such as DNA replication, and RNA ..
  85. ncbi Analysis of sequence-dependent curvature in matrix attachment regions
    J Yamamura
    Institute of Agriculture and Forestry, University of Tsukuba, Ibaraki 305 8572, Tsukuba, Japan
    FEBS Lett 489:166-70. 2001
    ..These results suggest that DNAs of MARs have a characteristic conformation instead of conserved sequence...
  86. ncbi Regulating the mammalian genome: the role of nuclear architecture
    Ronald Berezney
    Department of Biological Sciences, State University of New York at Buffalo, 14260, USA
    Adv Enzyme Regul 42:39-52. 2002
  87. ncbi Impaired nuclear translocation, nuclear matrix targeting, and intranuclear mobility of mutant androgen receptors carrying amino acid substitutions in the deoxyribonucleic acid-binding domain derived from androgen insensitivity syndrome patients
    Hisaya Kawate
    Department of Geriatric Medicine, Graduate School of Medical Sciences, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
    J Clin Endocrinol Metab 90:6162-9. 2005
    ..In this study, we examined whether intracellular dynamics of mutant ARs detected in two androgen insensitivity syndrome (AIS) patients was impaired...
  88. ncbi The KRAB zinc finger gene ZNF74 encodes an RNA-binding protein tightly associated with the nuclear matrix
    B Grondin
    Institut de Recherches Cliniques de Montreal, Universite de Montreal, Montreal, Quebec H2W 1R7, Canada
    J Biol Chem 271:15458-67. 1996
    ..Subcellular and in situ sequential fractionations further showed that ZNF74 is associated with the nuclear matrix. The RNA binding properties of this protein and its tight association with the nuclear matrix, a subnuclear ..
  89. pmc DNA-PK-dependent binding of DNA ends to plasmids containing nuclear matrix attachment region DNA sequences: evidence for assembly of a repair complex
    Stanley K Mauldin
    Lankenau Institute for Medical Research, 100 Lancaster Avenue, Wynnewood, PA 19096, USA and Genisphere, Incorporated, 4170 City Avenue, Philadelphia, PA 19131 1694, USA
    Nucleic Acids Res 30:4075-87. 2002
    ..Competition experiments between pUC18 and pUC18 plasmids containing various nuclear matrix attachment region (MAR) sequences suggest that DNA ends preferentially associate with plasmids containing MAR ..
  90. ncbi A nuclear matrix attachment region upstream of the T cell receptor beta gene enhancer binds Cux/CDP and SATB1 and modulates enhancer-dependent reporter gene expression but not endogenous gene expression
    S Chattopadhyay
    Center for Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    J Biol Chem 273:29838-46. 1998
    ..Furthermore, we demonstrate that HS1 is a nuclear matrix attachment region, referred to as MARbeta...
  91. ncbi DNA replication initiates at domains overlapping with nuclear matrix attachment regions in the xenopus and mouse c-myc promoter
    Claire Girard-Reydet
    Institute of Human Genetics, CNRS, Genome Dynamics and Development, 141, rue de la Cardonille, 34396 Montpellier Cedex 5, France
    Gene 332:129-38. 2004
    ..We also identified a nuclear matrix attachment region in both Xenopus and mouse, which is localized to two different regions of the c-myc promoter ..
  92. ncbi The homeodomain coordinates nuclear entry of the Lhx3 neuroendocrine transcription factor and association with the nuclear matrix
    G E Parker
    Department of Biology, Indiana University Purdue University, Indianapolis, Indiana 46202 5132, USA
    J Biol Chem 275:23891-8. 2000
    ..By using functional fluorescent derivatives, we demonstrate that Lhx3 is found in both the nucleoplasm and nuclear matrix. Three nuclear localization signals were mapped within the homeodomain, and one was located in the carboxyl ..
  93. ncbi p53 binds the nuclear matrix in normal cells: binding involves the proline-rich domain of p53 and increases following genotoxic stress
    M Jiang
    YCR P53 Research Group, Department of Biology, University of York, YO10 5DD, UK
    Oncogene 20:5449-58. 2001
    ..In mammalian cells the biochemical processing of DNA occurs on a nuclear sub-structure termed the nuclear matrix. Previously Deppert and co-workers have identified p53 in association with the nuclear matrix in viral- and ..
  94. ncbi Stabilization of the nuclear matrix by disulfide bridges: identification of matrix polypeptides that form disulfides
    N Stuurman
    E C Slater Institute for Biochemical Research, University of Amsterdam, The Netherlands
    Exp Cell Res 200:285-94. 1992
    The molecular structure of the nuclear matrix is still poorly understood. We have tried to assess which proteins are important structural elements by examining the process of stabilization of the nuclear matrix by sodium tetrathionate...
  95. ncbi Nuclear matrix protein mitotin messenger RNA is expressed at constant levels during the cell cycle
    I T Todorov
    Institut of Cell Biology and Morphology, Bulgarian Academy of Sciences, Sofia
    Biochem Biophys Res Commun 177:395-400. 1991
    During the course of an investigation on nuclear matrix protein cDNAs, we have isolated a cDNA clone hybridizing with the messenger RNA encoding mitotin. Mitotin is a 125 kDa/pI 6...
  96. ncbi Phosphorylation of AML1/RUNX1 regulates its degradation and nuclear matrix association
    Joseph R Biggs
    Department of Medicine, P O Box 250955, Medical University of South Carolina, 6 Jonathan Lucas Street, Charleston, SC 29425, USA
    Mol Cancer Res 3:391-401. 2005
    ..one soluble in buffers containing salt and nonionic detergent and the other insoluble and tightly bound to the nuclear matrix. We find that the AML1c protein is modified by both phosphorylation and ubiquitination...
  97. pmc Interaction of the tau2 transcriptional activation domain of glucocorticoid receptor with a novel steroid receptor coactivator, Hic-5, which localizes to both focal adhesions and the nuclear matrix
    L Yang
    Department of Pathology and Department of Biochemistry and Molecular Biology, University of Southern California, Los Angeles, California 90089, USA
    Mol Biol Cell 11:2007-18. 2000
    ..identified Hic-5 as a protein that interacts with a region of the glucocorticoid receptor that includes a nuclear matrix-targeting signal and the tau2 transcriptional activation domain...
  98. ncbi A possible role of cholesterol-sphingomyelin/phosphatidylcholine in nuclear matrix during rat liver regeneration
    Elisabetta Albi
    Department of Biochemical Sciences and Molecular Biotechnology, Physiopathology, Policlinico Monteluce, 06100 Perugia, Italy
    J Hepatol 38:623-8. 2003
    ..The aim of the present work is to clarify if chromatin lipids may derive or not from nuclear matrix and if they have different roles.
  99. ncbi The controversial nuclear matrix: a balanced point of view
    A M Martelli
    Dipartimento di Scienze Anatomiche Umane e Fisiopatologia dell Apparato Locomotore, Sezione di Anatomia, Universita di Bologna, Bologna, Italy
    Histol Histopathol 17:1193-205. 2002
    The nuclear matrix is defined as the residual framework after the removal of the nuclear envelope, chromatin, and soluble components by sequential extractions...
  100. ncbi DNA binding and gene activation properties of the Nmp4 nuclear matrix transcription factors
    Kitti Torrungruang
    Department of Periodontics, Indiana University School of Dentistry, Indianapolis, Indiana 46202, USA
    J Biol Chem 277:16153-9. 2002
    Splice variants of the Nmp4 gene include nuclear matrix transcription factors that regulate the type I collagen alpha1(I) polypeptide chain (COL1A1) promoter and several matrix metalloproteinase (MMP) genes...
  101. ncbi Co-localization of poly(ADPR)polymerase 1 (PARP-1) poly(ADPR)polymerase 2 (PARP-2) and related proteins in rat testis nuclear matrix defined by chemical cross-linking
    Filomena Tramontano
    Department of Biological Chemistry, University Federico II of Naples, Via Mezzocannone 16, 80134 Napoli, Italy
    J Cell Biochem 94:58-66. 2005
    ..Moreover, the association of pADPR and PARP-1 with the nuclear matrix (NM) has been reported, based on the poly(ADP-ribosyl)ation of nuclear matrix proteins (NMPs)...

Research Grants62

  1. Chymase-Mediated MMP Activation in Ishemia Reperfusion Injury
    LOUIS JOSEPH DELL'ITALIA; Fiscal Year: 2013
    ..Recent studies demonstrated activated MMP-2 within the cardiomyocyte that cleaved myofibrils (4-6) and nuclear matrix protein poly ADP-ribose polymerase (PARP)-a DNA repair enzyme in cell survival during I/R (7)...
  2. PAX2 gene targets, binding sites and function
    Patrick D Brophy; Fiscal Year: 2010
    ..Further experiments will focus on target gene association with nuclear matrix, which also is known to provide a change from random to specific anchorage points when chromatin becomes ..
  3. Checkpoints of TNF Gene Regulation
    Anne Goldfeld; Fiscal Year: 2013
    ..DNase hypersensitivity sites (HSSs), intrachromosomal interactions, and interactions with the nuclear matrix. The identification of potential therapeutic targets to control TNF expression in specific cell types and in ..
  4. Contribution of Sperm Nucleus to Paternal DNA Replication
    WILLIAM S WARD; Fiscal Year: 2013
    ..In Specific Aim 3 we will test whether ORC2L-5L or ORC1L binds directly to the sperm nuclear matrix, using ORC-GFP fusion proteins...
  5. Repair of oxidative damage in mammalian genomes
    Tapas K Hazra; Fiscal Year: 2010
    ..mutations in NEIL2-deficient cells, are consistent with this hypothesis, hnRNP-U, an abundant multifunctional nuclear matrix protein, has also been shown to regulate transcription. NEIL2 carries out a beta-delta-reaction like E...
  6. Elucidating the role of TIN2L at the telomere and in dyskeratosis congenita
    Nya D Nelson; Fiscal Year: 2013
    ..TIN2L associates with the telomeres and the nuclear matrix, indicating that it is unlikely to have identical function to TIN2S...
  7. A TRANSCRIPTION FACTOR WITHIN LIPID RAFTS MODULATES B CELL SIGNALING VIA THE BCR
    Philip W Tucker; Fiscal Year: 2010
    ..transcription factor that transactivates the immunoglobulin heavy chain (IgH) locus by binding to nuclear matrix attachment regions flanking the IgH intronic enhancer...
  8. Epigenetic Regulation in Self-Renewing and Differentiating Male Germ Cells
    CHRISTOPHER JESS PAYNE; Fiscal Year: 2012
    ..The second aim will examine the role of nuclear matrix proteins in the maintenance of selfrenewing spermatogonia...
  9. Functional Analysis of the Hairless Protein
    Angela M Christiano; Fiscal Year: 2012
    ..We also demonstrated that Hr is tightly associated with the nuclear matrix and forms nuclear speckles in association with the histone deacetylase, HDAC3...
  10. Anaplasma regulation of host granulocyte function
    John Stephen Dumler; Fiscal Year: 2013
    ..reprograms the global neutrophil transcriptome by altering the epigenome through AnkA"s action on nuclear matrix, chromatin, and transcriptional apparatus recruitment. We propose the following aims: 1...
  11. Understanding the Regulation of Megakaroblastic Leukemia 1 (MLK1)
    THERA C ALEXIS; Fiscal Year: 2010
    ..SAP region's function is not well understood but has been involved in nuclear matrix attachment in other proteins...
  12. KSHV-Ori-Lyt-Dependent DNA Replication
    Yan Yuan; Fiscal Year: 2012
    ..Two cellular proteins that may involve origin DNA unwinding and nuclear matrix attachment will be further studied because these two events are believed to be antecedent steps before ..
  13. Mechanism &Function of Subnuclear Targeting of Transcription Factors in Bone
    Gary S Stein; Fiscal Year: 2011
    ..Our program initially identified Runx2 as the osteoblast-specific nuclear matrix protein NMP2...
  14. Boston University Cross-Disciplinary Training in Nanotechnology for Cancer
    Bennett B Goldberg; Fiscal Year: 2013
    ..Proteomics in particular is emerging as a tool for detection of nuclear matrix proteins and new biomarkers for screening of early tumors stage...
  15. Serum Proteomic Biomarkers for Colon & Pancreatic Cancer
    William Bigbee; Fiscal Year: 2009
    ..Two major Projects are proposed: (1) Nuclear Matrix Proteins for Early Detection of CRC, and (2) Luminex Multianalyte Profiling (LabMAP(r)) and SELDI-TOF-MS Serum ..
  16. Preclinical assay development of a novel Colon Cancer Marker (CCSA-4)
    JACK CLEMENS; Fiscal Year: 2007
    ..Onconome, Inc. has licensed these novel nuclear matrix proteins and is developing products for the detection of these biomarkers for research and clinical use...
  17. Refinement and Discovery of Nuclear Matrix Protein Markers for Colorectal Cancer
    Robert E Schoen; Fiscal Year: 2013
    ..applicant): The focus of this proposal is to continue development of a unique panel of colon cancer associated nuclear matrix proteins (NMPs), discovered and developed during our previous EDRN-sponsored, Biomarker Development Laboratory ..
  18. ANTIMETOBOLITES AND NUCLEAR MATRIX-BOUND DNA REPLICATION
    Daniel Fernandes; Fiscal Year: 1991
    ..Recent evidence supports the concept that in eucaryotic cells DNA replication are attached to fixed sites on the nuclear matrix, and that template DNA passes through these sites as it is replicated...
  19. ROLE OF TESTIS-SPECIFIC HISTONES IN SPERMATOGENESIS
    Chi Bom Chae; Fiscal Year: 1993
    ..TH2B genes, presence of testis-specific dominant control region near the domain, definition of the domain by nuclear matrix binding sites, and regulation of testis-specific histone genes by the testis-specific enhancer activity of the ..
  20. CELLULAR PROCESSING OF DAMAGED DNA--ROLE IN ONCOGENESIS
    Philip Hanawalt; Fiscal Year: 2000
    ..b>Nuclear matrix associated DNA will be characterized to determine whether that is the site of transcription-coupled repair...
  21. AUTOANTIBODIES TO CELLULAR MATRIX ANTIGENS IN CFS
    ENG TAN; Fiscal Year: 2000
    ..Cellular structures (nuclear envelope, vimentin-containing intermediate filaments and a nuclear matrix particle visualized in immunofluorescence as reticulated speckles) associated with these antigens contain ..
  22. Proteomic Characterization of IC Bladder
    Pradeep Tyagi; Fiscal Year: 2006
    ..The nuclear matrix is the structural scaffolding of the cell nucleus and plays a central role in the regulation of important ..
  23. ANTIBODIES TO NUCLEAR PROTEINS IN CERVICAL CANCER
    EDWARD FEY; Fiscal Year: 1992
    ..We have fractionated Pap smear samples and purified nuclear matrix proteins from normal patients and from patients with carcinoma in situ...
  24. CHROMATIN-TELOMERE STRUCTURE AND AT GENOMIC SENSITIVITY
    TEJ PANDITA; Fiscal Year: 2005
    ..We demonstrated that altered telomere nuclear matrix interactions and nucleosomal periodicity observed in cells derived from individuals with ataxia telangiectasia ..
  25. MATRIX ATTACHMENT REGIONS IN AN IMPRINTED DOMAIN
    John Greally; Fiscal Year: 2001
    ..MARs are DNA sequences which anchor chromatin to a fixed nuclear matrix. Many nuclear factors (such as transcriptional and replication enzymes) are associated with the nuclear matrix,..
  26. L-TRIIODOTHYRONINE EFFECTS ON CELL REGULATION
    Martin Surks; Fiscal Year: 1992
    ..shown that T3 nuclear receptor concentration is augmented in S phase of the cell cycle and is localized to the nuclear matrix, a DNA and salt resistant nuclear fraction that is associated with new DNA synthesis and transcription ..
  27. NUTRITION AND PROTEIN METABOLISM IN TUMOR AND HOST
    Nawfal Istfan; Fiscal Year: 2000
    ..The latter is thought to impact on the organization of the nuclear matrix which has been related to DNA replication origins through specific attachments (scaffold attachment regions, ..
  28. INTERACTION OF CIS-ACTING ELEMENTS IN CD8 REGULATION
    Philip Tucker; Fiscal Year: 2005
    ..The L2a element is a nuclear matrix associated region (or MAR). Interestingly, inclusion of both the E81 enhancer and a 4...
  29. FUNCTION OF MATRIX ATTACHMENT REGIONS IN THE IG LOCI
    ANN FEENEY; Fiscal Year: 2000
    Matrix attachment regions (MAR) are AT-rich regions of DNA which mediate attachment of the chromosome to the nuclear matrix. MARs have been studied in a few model loci, two of which are the IgH and Ig- kappa loci...
  30. HEAT AND RADIATION INDUCED CHANGES IN THE NUCLEAR MATRIX
    JOSEPH DYNLACHT; Fiscal Year: 2000
    The nuclear matrix is important for defining nuclear structure and regulating metabolic processes such as DNA and RNA synthesis, and possibly DNA repair...
  31. TOPOISOMERASE DRUG ACTIONS AT NUCLEAR MATRIX DNA DOMAIN
    Daniel Fernandes; Fiscal Year: 2003
    DESCRIPTION: (Applicant's Abstract) The nuclear matrix model of DNA replication and organization will be utilized in the proposed studies to address (a) important effects of topoisomerase-active drugs on primary and higher order ..
  32. EFFECTS OF CHROMATIN ARCHITECTURE ON ORIGIN ACTIVITY
    PIETER DIJKWEL; Fiscal Year: 2003
    The long-range goal is to define the role of chromosome architecture, and in particular interaction with the nuclear matrix, in the regulation of replication origin activity in mammalian cells...
  33. The Fate of dsRNA in the Nucleus
    Gordon Carmichael; Fiscal Year: 2006
    ..This complex contains the inosine-specific RNA binding protein p54nrb, the splicing factor PSF and the inner nuclear matrix structural protein matrin 3...
  34. CELLULAR MECHANISMS OF HYPERTHERMIA DAMAGE
    RONALD COSS; Fiscal Year: 1992
    ..These structural systems are: 1) the cytomatrix; 2) the nuclear envelope/lamina complex; and 3) the nuclear matrix. The major aim will be to test the hypothesis that unrepaired heat-induced structural damage to these systems ..
  35. Base unpairing regions and chromatin folding
    Terumi Kohwi Shigematsu; Fiscal Year: 2004
    ..to be accomplished by chromatin making periodic attachments to the residual framework of nuclei, called the nuclear matrix, at specialized DNA sequences called matrix attachment regions, or MARs...
  36. STUDIES OF NORMAL AND NEOPLASTIC PROSTATE
    Khalil Ahmed; Fiscal Year: 1993
    ..of certain nuclear cAMP-independent PKs which may be involved in the phosphorylation of euchromatin NHP, nuclear matrix NHP, and androgen receptor...
  37. BIOCHEMISTRY OF LATE MITOTIC EVENTS IN CULTURED CELLS
    LON HODGE; Fiscal Year: 1980
    ..phospholipid precursors to probe nuclear envelope reformation and by use of a defined nuclear subfraction, the nuclear matrix, of probe reformation of other nuclear structures...
  38. Nanopatterned Surfaces to Control Cell Fate
    KEVIN HEALY; Fiscal Year: 2006
    ..mechanical forces transmitted via the direct link of the cytoskeleton to the nucleus, and in particular to nuclear matrix proteins (NMPs) such as NMP 1 and 2...
  39. MOLECULAR MECHANISM OF MINERALOCORTICOID RECEPTOR ACTION
    Geza Fejes Toth; Fiscal Year: 2003
    ..Our studies also indicate that MR clusters are associated with the nuclear matrix but not with pallindromic hormone response elements...
  40. Molecular Genetic Study of Repair of Radiation Damage
    THOMAS STAMATO; Fiscal Year: 2003
    ..DNA breaks and interacts with DNA-PKcs/Ku to direct the association of DNA ends with MAR DNA sequences on the nuclear matrix. Aim 1 is to Investigate the involvement of the human PSO4 protein in DNA-PKcS/Ku mediated association of DNA ..
  41. INTRACELLULAR MECHANISMS OF GLUCOCORTICOID ACTION
    Donald Defranco; Fiscal Year: 2005
    ..ECM) effects on gene regulatory events are hypothesized to occur, in part, through a direct effect on the nuclear matrix. The role of the ECM in several biological processes is now well established and much is known about the ..
  42. REGULATION OF GLUCOCORTICOID RECEPTOR GENE EXPRESSION
    WAYNE VEDECKIS; Fiscal Year: 2002
    ..the: role that the transcription factor YY1 plays in regulating GR gene transcription; possible involvement of nuclear matrix association in this process; identification of the GR promoter sequences that regulate cell cycle dependent ..
  43. CELL-TYPE SPECIFIC SUPPRESSION OF THE IGH ENHANCER
    Richard Scheuermann; Fiscal Year: 1999
    ..DNA binding and repressor function, and to investigate the functional interaction between NF-muNR and the nuclear matrix in transgenic mice...
  44. HOXBOXES, HOMEODOMAINS, AND OSTEOBLAST GENE EXPRESSION
    Dwight Towler; Fiscal Year: 2007
    ..that enhances Runx2-dependent OC transcription; and (b) MINT (Msx2 interacting nuclear target), an RRM domain nuclear matrix protein that we discovered by interaction cloning...
  45. NON B DNA STRUCTURE WITH CHEMICAL CARCINOGENS
    Terumi Kohwi Shigematsu; Fiscal Year: 2001
    ..It is believed that chromatin loops are anchored to the nuclear matrix at specific DNA regions (Matrix attachment regions, or MARs) that exhibit high affinity to the nuclear matrix...
  46. SPINAL CORD INJURY AND BLADDER CANCER DETECTION
    Robert Getzenberg; Fiscal Year: 1999
    ..The investigators have recently identified nuclear matrix proteins (NMPs) with which it is possible to differentiate human bladder tumors from normal bladder epithelial ..
  47. NUCLEAR CORE FILAMENTS
    Sheldon Penman; Fiscal Year: 2001
    DESCRIPTION: (Adapted from applicant's abstract). Embedment-free electron microscopy shows the nuclear matrix comprised of a network of 11 nm filaments with numerous embedded dense organelles...
  48. GENE EXPRESSION OF THE YEAST PLASMID 2 MICRON CIRCLE
    James Broach; Fiscal Year: 1991
    ..We have purified the REP1 protein and shown that it is a component of the yeast nuclear matrix. Further, we have found that it undergoes calcium induced aggregation and binds specifically to discrete ..
  49. ROLE OF THE NUCLEAR MATRIX PROTEIN NRB/B IN BRAIN
    Shalom Avraham; Fiscal Year: 2002
    DESCRIPTION(Verbatim from Applicant's Abstract): The nuclear matrix is defined as the insoluble framework of the nucleus and has been implicated in modulating gene expression, the cell cycle and nuclear structural integrity via linkage ..
  50. NUCLEAR PROTEINS AND REGULATION OF LYMPHOCYTE GROWTH
    Nili Feuerstein; Fiscal Year: 1993
    ..Recently, we have shown that a nuclear protein which is tightly associated with the nuclear matrix (termed "numatrin") may have an important role in the regulation of cell growth in normal and in neoplastic ..
  51. NUCLEAR MATRIX STRUCTURES AND GENOMIC FUNCTION
    Ronald Berezney; Fiscal Year: 2003
    ..This organization is strikingly maintained following extraction of cells for nuclear matrix under conditions which maintained the DNA intact...
  52. NUCLEAR MATRIX STRUCTURES AND GENOMIC FUNCTION
    Ronald Berezney; Fiscal Year: 2002
    ..This organization is strikingly maintained following extraction of cells for nuclear matrix under conditions which maintained the DNA intact...
  53. Epigenetic Regulation in Self-Renewing and Differentiating Male Germ Cells
    Christopher Payne; Fiscal Year: 2009
    ..The second aim will examine the role of nuclear matrix proteins in the maintenance of self-renewing spermatogonia...
  54. NEW NUCLEAR MATRIX PROTEIN IN BREAST CANCER GROWTH
    Steffi Oesterreich; Fiscal Year: 2002
    ..she cloned a novel protein (HET) which apparently is identical to a recently cloned scaffold attachment factor/nuclear matrix protein...
  55. NUCLEAR MATRIX AND ACTIN--BIOMARKERS FOR BLADDER CANCER
    Robert Veltri; Fiscal Year: 1992
    Cytoskeletal and nuclear matrix proteins (NMPs) play an important role in the bio-chemical events involved in differentiation and transformation of mammalian cells...
  56. TRANSCRIPTIONAL REGULATION AND TUMORIGENESIS BY MMTV
    JAQUELIN DUDLEY; Fiscal Year: 2002
    ..Two nuclear protein complexes bind to the NREs. Those complexes contain the nuclear matrix-associated proteins SATB1 and Cux/CDP...
  57. Nuclear Matrix Structure and Genomic Function
    Ronald Berezney; Fiscal Year: 2007
    ..The striking structural preservation of a variety of genomic processes on the nuclear matrix provides an important approach for correlating nuclear form and function...
  58. COLLAGEN IN OSTEOARTHRITIC CARTILAGE
    PAUL BENYA; Fiscal Year: 1991
    ..modifications in microfilament structure are physically transmit:ed into the nucleus, by way of cytoskeletal-nuclear matrix interactions, and result in changes in gene expression, and the possibility that modification may generate ..
  59. TARGETING CELLULAR ONCOGENES WITH DNA-REACTIVE DRUGS
    Jan Woynarowski; Fiscal Year: 2000
    ..this application is to explore the possibility that AT-specific DNA-reactive drugs could preferentially target nuclear matrix-bound oncogene domains...
  60. GENETIC MECHANISMS THAT POTENTIATE HUMAN SPERMATOGENESIS
    STEPHEN KRAWETZ; Fiscal Year: 2009
    ..As we and others have shown, a likely key player in this mechanism of transcriptional regulation is the nuclear matrix attachment region...
  61. NUCLEAR TARGETING OF SIGNAL TRANSDUCTION ENZYMES
    VINCENT COGHLAN; Fiscal Year: 2000
    ..have discovered a novel anchoring protein, called AKAP 95, that binds PKA and is exclusively localized to the nuclear matrix. The properties of this AKAP will be characterized as part of a long term goal to define the relationship ..
  62. PURIFICATION OF A BONE NUCLEAR MATRIX PROTEIN
    JOSEPH BIDWELL; Fiscal Year: 1999
    ..These studies are designed to test the hypothesis that nuclear matrix protein 4 (NMP4) a novel, PTH-responsive osteoblast nuclear matrix factor, plays a role in promoting ..