nuclear matrix

Summary

Summary: The residual framework structure of the CELL NUCLEUS that maintains many of the overall architectural features of the cell nucleus including the nuclear lamina with NUCLEAR PORE complex structures, residual CELL NUCLEOLI and an extensive fibrogranular structure in the nuclear interior. (Advan. Enzyme Regul. 2002; 42:39-52)

Top Publications

  1. ncbi HA95 is a protein of the chromatin and nuclear matrix regulating nuclear envelope dynamics
    S B Martins
    Institute of Medical Biochemistry, University of Oslo, PO Box 1112 Blindern, Norway
    J Cell Sci 113:3703-13. 2000
  2. ncbi Internal organisation of the nucleus: assembly of compartments by macromolecular crowding and the nuclear matrix model
    Ronald Hancock
    Laval University Cancer Research Centre, Hotel Dieu Hospital, 9 rue MacMahon, Quebec, P Q G1R 2J6, Canada
    Biol Cell 96:595-601. 2004
  3. ncbi Nuclear matrix support of DNA replication
    Boyka Anachkova
    Institute of Molecular Biology, Bulgarian Academy of Sciences, Akad G Bonchev Street, Bl 21, Sofia 1113, Bulgaria
    J Cell Biochem 96:951-61. 2005
  4. pmc PIASy, a nuclear matrix-associated SUMO E3 ligase, represses LEF1 activity by sequestration into nuclear bodies
    S Sachdev
    Gene Center and Institute of Biochemistry, University of Munich, 81377 Munich, Germany
    Genes Dev 15:3088-103. 2001
  5. pmc A novel DNA-binding motif in the nuclear matrix attachment DNA-binding protein SATB1
    K Nakagomi
    La Jolla Cancer Research Foundation, California 92037
    Mol Cell Biol 14:1852-60. 1994
  6. doi The sperm nucleus: chromatin, RNA, and the nuclear matrix
    Graham D Johnson
    The Center for Molecular Medicine and Genetics, C S Mott Center, Wayne State University of Medicine, 275 East Hancock, Detroit, MI 48201, USA
    Reproduction 141:21-36. 2011
  7. pmc A hyperphosphorylated form of the large subunit of RNA polymerase II is associated with splicing complexes and the nuclear matrix
    M J Mortillaro
    Department of Biological Sciences, State University of New York at Buffalo 14260, USA
    Proc Natl Acad Sci U S A 93:8253-7. 1996
  8. pmc CENP-F is a protein of the nuclear matrix that assembles onto kinetochores at late G2 and is rapidly degraded after mitosis
    H Liao
    Fox Chase Cancer Center, Institute for Cancer Research, Philadelphia, Pennsylvania 19111, USA
    J Cell Biol 130:507-18. 1995
  9. pmc A novel form of the telomere-associated protein TIN2 localizes to the nuclear matrix
    Patrick G Kaminker
    Buck Institute for Age Research, Novato, California, USA
    Cell Cycle 8:931-9. 2009
  10. ncbi Necdin interacts with the ribonucleoprotein hnRNP U in the nuclear matrix
    Hideo Taniura
    Division of Regulation of Macromolecular Functions, Institute for Protein Research, Osaka University, Yamadaoka 3 2, Suita, Osaka 565 0871, Japan
    J Cell Biochem 84:545-55. 2002

Research Grants

Detail Information

Publications289 found, 100 shown here

  1. ncbi HA95 is a protein of the chromatin and nuclear matrix regulating nuclear envelope dynamics
    S B Martins
    Institute of Medical Biochemistry, University of Oslo, PO Box 1112 Blindern, Norway
    J Cell Sci 113:3703-13. 2000
    ..Biochemical and photobleaching data indicate that HA95 is tightly associated with chromatin and the nuclear matrix/lamina network in interphase, and bound to chromatin at mitosis...
  2. ncbi Internal organisation of the nucleus: assembly of compartments by macromolecular crowding and the nuclear matrix model
    Ronald Hancock
    Laval University Cancer Research Centre, Hotel Dieu Hospital, 9 rue MacMahon, Quebec, P Q G1R 2J6, Canada
    Biol Cell 96:595-601. 2004
    ..nucleus are segregated into discrete compartments, but the current model that this is achieved by a fibrillar nuclear matrix which structures the nuclear interior and compartments is not consistent with all experimental observations, ..
  3. ncbi Nuclear matrix support of DNA replication
    Boyka Anachkova
    Institute of Molecular Biology, Bulgarian Academy of Sciences, Akad G Bonchev Street, Bl 21, Sofia 1113, Bulgaria
    J Cell Biochem 96:951-61. 2005
    ..To achieve this, DNA is organized into loops attached to the nuclear matrix. Each loop represents one individual replicon with the origin of replication localized within the loop and the ..
  4. pmc PIASy, a nuclear matrix-associated SUMO E3 ligase, represses LEF1 activity by sequestration into nuclear bodies
    S Sachdev
    Gene Center and Institute of Biochemistry, University of Munich, 81377 Munich, Germany
    Genes Dev 15:3088-103. 2001
    ..Moreover, PIASy binds to nuclear matrix-associated DNA sequences and targets LEF1 to nuclear bodies, suggesting that PIASy-mediated subnuclear ..
  5. pmc A novel DNA-binding motif in the nuclear matrix attachment DNA-binding protein SATB1
    K Nakagomi
    La Jolla Cancer Research Foundation, California 92037
    Mol Cell Biol 14:1852-60. 1994
    The nuclear matrix attachment DNA (MAR) binding protein SATB1 is a sequence context-specific binding protein that binds in the minor groove, making virtually no contact with the DNA bases...
  6. doi The sperm nucleus: chromatin, RNA, and the nuclear matrix
    Graham D Johnson
    The Center for Molecular Medicine and Genetics, C S Mott Center, Wayne State University of Medicine, 275 East Hancock, Detroit, MI 48201, USA
    Reproduction 141:21-36. 2011
    ..These regions may be necessary for organizing higher order genomic structure through interactions with the nuclear matrix. The promoters of this transcriptionally quiescent genome are differentially marked by modified histones that ..
  7. pmc A hyperphosphorylated form of the large subunit of RNA polymerase II is associated with splicing complexes and the nuclear matrix
    M J Mortillaro
    Department of Biological Sciences, State University of New York at Buffalo 14260, USA
    Proc Natl Acad Sci U S A 93:8253-7. 1996
    ..retained in a similar pattern following in situ extraction of cells and was quantitatively recovered in the nuclear matrix fraction...
  8. pmc CENP-F is a protein of the nuclear matrix that assembles onto kinetochores at late G2 and is rapidly degraded after mitosis
    H Liao
    Fox Chase Cancer Center, Institute for Cancer Research, Philadelphia, Pennsylvania 19111, USA
    J Cell Biol 130:507-18. 1995
    ..CENP-F is protein of the nuclear matrix that gradually accumulates during the cell cycle until it reaches peak levels in G2 and M phase cells and is ..
  9. pmc A novel form of the telomere-associated protein TIN2 localizes to the nuclear matrix
    Patrick G Kaminker
    Buck Institute for Age Research, Novato, California, USA
    Cell Cycle 8:931-9. 2009
    ..TIN2S) in two ways: TIN2L contains an additional 97 amino acids, and TIN2L associates strongly with the nuclear matrix. Stringent salt and detergent conditions failed to extract TIN2L from the nuclear matrix, despite removing ..
  10. ncbi Necdin interacts with the ribonucleoprotein hnRNP U in the nuclear matrix
    Hideo Taniura
    Division of Regulation of Macromolecular Functions, Institute for Protein Research, Osaka University, Yamadaoka 3 2, Suita, Osaka 565 0871, Japan
    J Cell Biochem 84:545-55. 2002
    ..contained cDNA encoding a carboxyl-terminal portion of heterogeneous nuclear ribonucleoprotein U (hnRNP U), a nuclear matrix-associated protein that interacts with chromosomal DNA...
  11. ncbi Fanconi anemia proteins localize to chromatin and the nuclear matrix in a DNA damage- and cell cycle-regulated manner
    F Qiao
    Departments of Microbiology and Pediatrics, University of Virginia and the University of Virginia Health System, Charlottesville, Virginia 22908, USA
    J Biol Chem 276:23391-6. 2001
    ..Biochemical fractionation reveals that the FA proteins are found in nuclear matrix and chromatin and that treatment with mitomycin C results in increase of the FA proteins in nuclear matrix and ..
  12. pmc PDLIM2 suppresses human T-cell leukemia virus type I Tax-mediated tumorigenesis by targeting Tax into the nuclear matrix for proteasomal degradation
    Pengrong Yan
    University of Pittsburgh Cancer Institute, University of Pittsburgh Medical Center, PA, USA
    Blood 113:4370-80. 2009
    ..In addition, PDLIM2 recruited Tax from its functional sites into the nuclear matrix where the polyubiquitinated Tax was degraded by the proteasome...
  13. ncbi From DNA structure to gene expression: mediators of nuclear compartmentalization and dynamics
    J Bode
    GBF German Research Center for Biotechnology Epigenetic Regulation, Mascheroder Weg 1, D 38124 Braunschweig
    Chromosome Res 11:435-45. 2003
    ..into chromatin domains by their attachment to a supporting structure that has traditionally been termed the nuclear matrix. Present evidence indicates the dynamics of this entity, which requires particular properties of the elements ..
  14. pmc Anchoring the genome
    Diego Ottaviani
    Cancer Research UK London Research Institute, Lincoln s Inn Fields, London WC2A 3PX, UK
    Genome Biol 9:201. 2008
    ..is evidence that higher-order chromatin folding is mediated by the anchoring of specific DNA sequences to the nuclear matrix. These genome anchors are also crucial regulators of gene expression and DNA replication, and play a role in ..
  15. ncbi An intact sperm nuclear matrix may be necessary for the mouse paternal genome to participate in embryonic development
    W S Ward
    Division of Urology, Robert Wood Johnson Medical School, New Brunswick, New Jersey 08903, USA
    Biol Reprod 60:702-6. 1999
    ..We also tested the stability of the sperm nuclear matrix by the ability to form nuclear halos. Sperm nuclei washed in freshly prepared 0...
  16. pmc Differential nuclear scaffold/matrix attachment marks expressed genes
    Amelia K Linnemann
    The Center for Molecular Medicine and Genetics, Wayne State University School of Medicine, C S Mott Center, Detroit, MI48201, USA
    Hum Mol Genet 18:645-54. 2009
    ..The varied functions of the S/MARs as revealed by the different extraction methods highlights their unique functional contribution...
  17. pmc Dynamics of association of origins of DNA replication with the nuclear matrix during the cell cycle
    V Djeliova
    Institute of Molecular Biology, Bulgarian Academy of Sciences, Akad. G. Bonchev Street, Bl. 21, Sofia 1113, Bulgaria
    Nucleic Acids Res 29:3181-7. 2001
    DNA of replication foci attached to the nuclear matrix was isolated from Chinese hamster ovary cells and human HeLa cells synchronized at different stages of the G(1) and S phases of the cell cycle...
  18. ncbi Functional domains of necdin for protein-protein interaction, nuclear matrix targeting, and cell growth suppression
    Hideo Taniura
    Graduate School of Natural Science and Technology, Kanazawa University, Kakumamachi, Kanazawa, Ishikawa 920 1192, Japan
    J Cell Biochem 94:804-15. 2005
    ..We here characterize the regions of necdin required for the protein-protein interaction, nuclear matrix targeting, and cell growth suppression...
  19. doi Accumulation of LANA at nuclear matrix fraction is important for Kaposi's sarcoma-associated herpesvirus replication in latency
    Eriko Ohsaki
    Department of Infectious Diseases, University of Hamamatsu School of Medicine, 1 20 1 Handayama, Higashi ku, Hamamatsu, Shizuoka 431 3192, Japan
    Virus Res 139:74-84. 2009
    ..the KSHV genome, and a viral replication factor, latency-associated nuclear antigen (LANA) accumulate at the nuclear matrix fraction in the G1 phase...
  20. pmc Human telomeres are attached to the nuclear matrix
    T de Lange
    Rockefeller University, New York, NY 10021
    EMBO J 11:717-24. 1992
    This report shows that human telomeres are tightly associated with the nuclear matrix. Telomere attachment is observed in several cell types and in all stages of interphase...
  21. doi Localization of prohibitin in the nuclear matrix and alteration of its expression during differentiation of human neuroblastoma SK-N-SH cells induced by retinoic acid
    Qi fu Li
    The Key Laboratory of Ministry of Education for Cell Biology and Tumor Cell Engineering, School of Life Science, Xiamen University, China
    Cell Mol Neurobiol 31:203-11. 2011
    The nuclear matrix-intermediate filament system of human neuroblastoma SK-N-SH cells before and after retinoic acid (RA) treatment was selectively extracted and the distribution of prohibitin (PHB) in the nuclear matrix, as well as its ..
  22. pmc Structural insights into SUN-KASH complexes across the nuclear envelope
    Wenjia Wang
    State Key Laboratory of Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China
    Cell Res 22:1440-52. 2012
    ..These results provide a structural model of the LINC complex, which is essential for additional study of the physical and functional coupling between the cytoplasm and the nucleoplasm...
  23. ncbi Function of the sperm nuclear matrix
    Jeffrey A Shaman
    Institute for Biogenesis Research, John A Burns School of Medicine, University of Hawaii Honolulu, HI 96822, USA
    Arch Androl 53:135-40. 2007
    ..One of these is the organization of DNA into loop domains attached at their bases to a proteinaceous nuclear matrix. Several groups have shown that the sites at which DNA associates with the sperm nuclear matrix contain ..
  24. ncbi The sperm nuclear matrix is required for paternal DNA replication
    Jeffrey A Shaman
    Institute for Biogenesis Research, John A Burns School of Medicine, University of Hawaii, Honolulu, Hawaii 96822, USA
    J Cell Biochem 102:680-8. 2007
    ..We previously demonstrated that mammalian sperm nuclei contain a nuclear matrix that organizes the DNA into loop domains in a manner similar to that of somatic cells...
  25. pmc S/MARt DB: a database on scaffold/matrix attached regions
    Ines Liebich
    Research Group Bioinformatics, GBF, Mascheroder Weg 1, D 38124 Braunschweig, Germany
    Nucleic Acids Res 30:372-4. 2002
    ..S/MAR transaction database, is a relational database covering scaffold/matrix attached regions (S/MARs) and nuclear matrix proteins that are involved in the chromosomal attachment to the nuclear scaffold...
  26. ncbi Dynamics of nuclear matrix proteome during embryonic development in Drosophila melanogaster
    Parul Varma
    Center for Cellular and Molecular Biology CSIR, Hyderabad 500 007, India
    J Biosci 36:439-59. 2011
    ..of nuclear architecture, which is believed to play an important role in regulation of gene expression, is the nuclear matrix (NuMat)...
  27. doi Exploring the relationship between interphase gene positioning, transcriptional regulation and the nuclear matrix
    Lauren S Elcock
    Laboratory of Nuclear and Genomic Health, Centre for Cell and Chromosome Biology, Brunel University, Uxbridge UB8 3PH, UK
    Biochem Soc Trans 38:263-7. 2010
    ..at a more local scale? During the course of this review, these questions will be considered, in light of the current literature regarding the role of transcription factories and the nuclear matrix in interphase genome organization.
  28. ncbi DNA damage-dependent interaction of the nuclear matrix protein C1D with Translin-associated factor X (TRAX)
    Tuba Erdemir
    Bilkent University, Molecular Biology and Genetics Department, 06533, Bilkent, Ankara, Turkey
    J Cell Sci 115:207-16. 2002
    The nuclear matrix protein C1D is an activator of the DNA-dependent protein kinase (DNA-PK), which is essential for the repair of DNA double-strand breaks (DSBs) and V(D)J recombination...
  29. ncbi A new spectrin, beta IV, has a major truncated isoform that associates with promyelocytic leukemia protein nuclear bodies and the nuclear matrix
    W T Tse
    Division of Hematology Oncology, Children s Hospital, and the Dana Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 276:23974-85. 2001
    ..Spectrin betaIV is the first beta-spectrin associated with a subnuclear structure and may be part of a nuclear scaffold to which gene regulatory machinery binds...
  30. pmc A structural basis for cellular senescence
    Armando Aranda-Anzaldo
    Laboratorio de Biologia Molecular, Facultad de Medicina, Universidad Autonoma del Estado de Mexico, Paseo Tollocan y Jesús Carranza, Toluca, Edo Mex, Mexico
    Aging (Albany NY) 1:598-607. 2009
    ..and discuss evidence that the interactions between DNA and the nuclear substructure, commonly known as the nuclear matrix, define a higher-order structure within the cell nucleus that following thermodynamic constraints, ..
  31. doi Aged and post-mitotic cells share a very stable higher-order structure in the cell nucleus in vivo
    Janeth Alva-Medina
    Universidad Autonoma del Estado de Mexico, Toluca, Estado de Mexico, Mexico
    Biogerontology 11:703-16. 2010
    ..cells the nuclear DNA is organized in supercoiled loops anchored to a proteinaceous substructure known as the nuclear matrix (NM). The DNA-NM interactions define a higher-order structure in the cell nucleus (NHOS)...
  32. ncbi Hcc-1 is a novel component of the nuclear matrix with growth inhibitory function
    C L Leaw
    Bioprocessing Technology Institute, Agency for Science, Technology and Research A STAR, Singapore
    Cell Mol Life Sci 61:2264-73. 2004
    ..0173) and an accumulation of cells at the G2/M phase (p = 0.0276 compared to control HEK293 cells). Taken together, these results suggest a role for Hcc-1 in growth regulation and nucleic acids metabolism...
  33. ncbi Ataxin-3 is transported into the nucleus and associates with the nuclear matrix
    D Tait
    Max Planck Institut fur Molekulare Genetik, Ihnestrasse 73, D 14195 Berlin Dahlem, Germany
    Hum Mol Genet 7:991-7. 1998
    ..revealed that the ataxin-3 protein present in the nucleus of neuroblastoma cells is associated with the inner nuclear matrix. Our results taken together with the finding of a nuclear localization signal in ataxin-3 indicate that the ..
  34. ncbi A new look at the nuclear matrix
    R Hancock
    Centre de recherche en cancérologie de l Université Laval, Hotel Dieu Hospital, Quebec, Canada
    Chromosoma 109:219-25. 2000
    The concept of the nuclear matrix, a karyoskeletal structure that serves as a support for the genome and its activities, has stimulated many studies of the association of nuclear components and functions with this structure...
  35. ncbi Experimental observations of a nuclear matrix
    J Nickerson
    Department of Cell Biology and Cancer Center, University of Massachusetts Medical School, Worcester, MA 01655, USA
    J Cell Sci 114:463-74. 2001
    ..architecture of the nucleus includes two overlapping and nucleic-acid-containing structures - chromatin and a nuclear matrix. The nuclear matrix is observed by microscopy in live, fixed and extracted cells...
  36. pmc The amino-terminal region of the retinoblastoma gene product binds a novel nuclear matrix protein that co-localizes to centers for RNA processing
    T Durfee
    Center for Molecular Medicine Institute of Biotechnology, University of Texas Health Science Center at San Antonio 78245
    J Cell Biol 127:609-22. 1994
    ..The only gene isolated from this screen encodes a novel 84-kD nuclear matrix protein that localizes to subnuclear regions associated with RNA processing...
  37. ncbi Nuclear matrix interactions within the sperm genome
    J A Kramer
    Department of Obstetrics and Gynecology, Center for Molecular Medicine and Genetics, Wayne State University School of Medicine, Detroit, Michigan 48102, USA
    J Biol Chem 271:11619-22. 1996
    ..human PRM1 --> PRM2 --> TNP2 genic domain has revealed two regions of attachment to the sperm nuclear matrix. These sperm nuclear matrix attachment regions delimit the DNase I-sensitive domain of this haploid-expressed ..
  38. pmc Silencing by nuclear matrix attachment distinguishes cell-type specificity: association with increased proliferation capacity
    Amelia K Linnemann
    Department of Obstetrics and Gynecology, The Center for Molecular Medicine and Genetics, Wayne State University School of Medicine, Detroit, MI 48201, USA
    Nucleic Acids Res 37:2779-88. 2009
    ..We have previously shown that attachment of genes to the NaCl-isolated nuclear matrix correlates with their silencing in HeLa cells...
  39. pmc Mapping of the nuclear matrix-bound chromatin hubs by a new M3C experimental procedure
    Alexey A Gavrilov
    Laboratory of Structural and Functional Organization of Chromosomes, Institute of Gene Biology of the Russian Academy of Sciences, 34 5 Vavilov Street, 119334 Moscow, Russia
    Nucleic Acids Res 38:8051-60. 2010
    We have developed an experimental procedure to analyze the spatial proximity of nuclear matrix-bound DNA fragments...
  40. pmc DNA moves sequentially towards the nuclear matrix during DNA replication in vivo
    Juan Carlos Rivera-Mulia
    Laboratorio de Biologia Molecular, Facultad de Medicina, Universidad Autonoma del Estado de Mexico, Apartado Postal 428, CP 50000 Toluca, Edo Mex, Mexico
    BMC Cell Biol 12:3. 2011
    In the interphase nucleus of metazoan cells DNA is organized in supercoiled loops anchored to a nuclear matrix (NM)...
  41. ncbi Nuclear matrix association of insulin receptor and IRS-1 by insulin in osteoblast-like UMR-106 cells
    Ki Cheon Seol
    Department of Pharmacology, School of Dentistry, Kyung Hee University, Seoul 130 701, Republic of Korea
    Biochem Biophys Res Commun 306:898-904. 2003
    ..The majority of insulin receptor and IRS-1 translocated to the nucleus appeared to associate with nuclear matrix. Tyrosine phosphorylation of a number of proteins with a molecular mass of 180, 95, 85, or 70 kDa in the ..
  42. pmc Sp2 localizes to subnuclear foci associated with the nuclear matrix
    K Scott Moorefield
    Graduate Program in Genomic Sciences, Center for Comparative Medicine and Translational Research, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27606, USA
    Mol Biol Cell 17:1711-22. 2006
    ..We report that 1) Sp2 localizes largely within subnuclear foci associated with the nuclear matrix, and 2) these foci are distinct from promyelocytic oncogenic domains and appear to be stable during an 18-h ..
  43. doi NeuN/Fox-3 is an intrinsic component of the neuronal nuclear matrix
    Myrna A R Dent
    Facultad de Medicina, Universidad Autonoma del Estado de Mexico, Toluca, Mexico, Mexico
    FEBS Lett 584:2767-71. 2010
    ..Here we provide evidence that NeuN/Fox-3 is an intrinsic component of the neuronal nuclear matrix and a reliable marker of nuclear speckles in neurons.
  44. ncbi Unraveling the organization of the internal nuclear matrix: RNA-dependent anchoring of NuMA to a lamin scaffold
    Paola Barboro
    Istituto Nazionale per la Ricerca sul Cancro, I 16132, Genova, Italy
    Exp Cell Res 279:202-18. 2002
    Using quantitative immunoelectron microscopy we show here that when the nuclear matrix is isolated from rat hepatocytes in the presence of an inhibitor of RNase activity both lamins and the nuclear mitotic apparatus protein (NuMA) ..
  45. ncbi Interaction of EBV latent origin of replication with the nuclear matrix: identification of S/MAR sequences and protein components
    Giulia Mearini
    Department of Public Health Sciences, University La Sapienza, Rome, Italy
    FEBS Lett 547:119-24. 2003
    During latency, Epstein Barr virus (EBV) genome, as an episome, is attached to the nuclear matrix (NM) via the latent origin of replication ori P...
  46. ncbi Interactions of Epstein-Barr virus origins of replication with nuclear matrix in the latent and in the lytic phases of viral infection
    E Mattia
    Faculty of Medicine and Surgery, University of Roma La Sapienza, Rome, Italy
    Virology 262:9-17. 1999
    Eukaryotic DNA is organized into domains or loops generated by the attachment of chromatin fibers to the nuclear matrix via specific regions called scaffold or matrix attachment regions...
  47. ncbi A novel CpG-free vertebrate insulator silences the testis-specific SP-10 gene in somatic tissues: role for TDP-43 in insulator function
    Mayuresh M Abhyankar
    Department of Pathology, University of Virginia School of Medicine, Charlottesville, Virginia 22908, USA
    J Biol Chem 282:36143-54. 2007
    ..Here we report that the insulator tethers the SP-10 gene to the nuclear matrix in somatic tissues, sequestering the core promoter in the process, thus preventing transcription...
  48. ncbi The newly identified human nuclear protein NXP-2 possesses three distinct domains, the nuclear matrix-binding, RNA-binding, and coiled-coil domains
    Yukio Kimura
    Department of Life Science, Graduate School and Faculty of Science, Himeji Institute of Technology, Kamigori, Hyogo 678 1201, Japan
    J Biol Chem 277:20611-7. 2002
    Using a monoclonal antibody that recognizes a nuclear matrix protein, we selected a cDNA clone from a lambdagt11 human placenta cDNA library. This cDNA encoded a 939-amino acid protein designated nuclear matrix protein NXP-2...
  49. ncbi c-Abl tyrosine kinase selectively regulates p73 nuclear matrix association
    Merav Ben-Yehoyada
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
    J Biol Chem 278:34475-82. 2003
    ..that in response to ionizing radiation, p73 undergoes nuclear redistribution and becomes associated with the nuclear matrix. This association is c-Abl-dependent because it was not observed in cells that are defective in c-Abl kinase ..
  50. pmc Gene positional changes relative to the nuclear substructure correlate with the proliferating status of hepatocytes during liver regeneration
    Apolinar Maya-Mendoza
    Laboratorio de Biologia Molecular, Facultad de Medicina, Universidad Autonoma del Estado de Mexico, Apartado Postal 428, C P 50000, Toluca, Edo Mex, Mexico
    Nucleic Acids Res 31:6168-79. 2003
    ..is organised in loops anchored to a proteinaceous substructure variously named but commonly known as the nuclear matrix. Important processes of nuclear physiology, such as replication, transcription and processing of primary ..
  51. ncbi Gene positional changes relative to the nuclear substructure during carbon tetrachloride-induced hepatic fibrosis in rats
    Apolinar Maya-Mendoza
    Laboratorio de Biologia Molecular, Facultad de Medicina, Universidad Autonoma del Estado de Mexico, Apdo Postal 428, C P 50000, Toluca, Edo Mex, Mexico
    J Cell Biochem 93:1084-98. 2004
    ..interphase nucleus the DNA of higher eukaryotes is organized in loops anchored to a substructure known as the nuclear matrix (NM)...
  52. pmc Autosomal-dominant distal myopathy associated with a recurrent missense mutation in the gene encoding the nuclear matrix protein, matrin 3
    Jan Senderek
    Institute of Cell Biology, ETH Zurich, 8093 Zurich, Switzerland
    Am J Hum Genet 84:511-8. 2009
    ..MATR3 is expressed in skeletal muscle and encodes matrin 3, a component of the nuclear matrix, which is a proteinaceous network that extends throughout the nucleus...
  53. ncbi Re-defining the chromatin loop domain
    H H Heng
    Center for Molecular Medicine and Genetics, Department of Pathology, Karmanos Cancer Institute, Wayne State University School of Medicine, Detroit MI 48202, USA
    Cytogenet Cell Genet 93:155-61. 2001
    ..to target some long-standing issues regarding the structure and function of the chromatin loop domain and its relationship with the nuclear matrix. This new knowledge will have a profound impact for modern genetics and molecular medicine.
  54. doi Exploring the effects of a dysfunctional nuclear matrix
    Lauren S Elcock
    Laboratory of Nuclear and Genomic Health, Centre for Cell and Chromosome Biology, Brunel University, Uxbridge, UK
    Biochem Soc Trans 36:1378-83. 2008
    The nuclear matrix has remained a contentious structure for decades; many believe that it is an artefact of harsh non-physiological procedures...
  55. pmc Boundary element-associated factor 32B connects chromatin domains to the nuclear matrix
    Rashmi U Pathak
    Department of Biochemistry, School of Life Sciences, University of Hyderabad, Hyderabad, India
    Mol Cell Biol 27:4796-806. 2007
    ..In this report we show that BEAF is associated with the nuclear matrix and map the domain required for matrix association to the middle region of the protein...
  56. ncbi Stabilization of the nuclear matrix by disulfide bridges: identification of matrix polypeptides that form disulfides
    N Stuurman
    E C Slater Institute for Biochemical Research, University of Amsterdam, The Netherlands
    Exp Cell Res 200:285-94. 1992
    The molecular structure of the nuclear matrix is still poorly understood. We have tried to assess which proteins are important structural elements by examining the process of stabilization of the nuclear matrix by sodium tetrathionate...
  57. ncbi p53 binds the nuclear matrix in normal cells: binding involves the proline-rich domain of p53 and increases following genotoxic stress
    M Jiang
    YCR P53 Research Group, Department of Biology, University of York, YO10 5DD, UK
    Oncogene 20:5449-58. 2001
    ..In mammalian cells the biochemical processing of DNA occurs on a nuclear sub-structure termed the nuclear matrix. Previously Deppert and co-workers have identified p53 in association with the nuclear matrix in viral- and ..
  58. ncbi Nuclear matrix bound fibroblast growth factor receptor is associated with splicing factor rich and transcriptionally active nuclear speckles
    Suryanarayan Somanathan
    Department of Biological Sciences, State University of New York at Buffalo, Buffalo, New York 14260, USA
    J Cell Biochem 90:856-69. 2003
    ..arrangements of FGFR1 sites and colocalization with nuclear speckles were maintained following extraction for nuclear matrix. Moreover, immunoblots indicated a significant enrichment of FGFR1 in the nuclear matrix fraction...
  59. ncbi Phosphorylation of AML1/RUNX1 regulates its degradation and nuclear matrix association
    Joseph R Biggs
    Department of Medicine, P O Box 250955, Medical University of South Carolina, 6 Jonathan Lucas Street, Charleston, SC 29425, USA
    Mol Cancer Res 3:391-401. 2005
    ..one soluble in buffers containing salt and nonionic detergent and the other insoluble and tightly bound to the nuclear matrix. We find that the AML1c protein is modified by both phosphorylation and ubiquitination...
  60. ncbi Nuclear matrix protein mitotin messenger RNA is expressed at constant levels during the cell cycle
    I T Todorov
    Institut of Cell Biology and Morphology, Bulgarian Academy of Sciences, Sofia
    Biochem Biophys Res Commun 177:395-400. 1991
    During the course of an investigation on nuclear matrix protein cDNAs, we have isolated a cDNA clone hybridizing with the messenger RNA encoding mitotin. Mitotin is a 125 kDa/pI 6...
  61. ncbi The controversial nuclear matrix: a balanced point of view
    A M Martelli
    Dipartimento di Scienze Anatomiche Umane e Fisiopatologia dell Apparato Locomotore, Sezione di Anatomia, Universita di Bologna, Bologna, Italy
    Histol Histopathol 17:1193-205. 2002
    The nuclear matrix is defined as the residual framework after the removal of the nuclear envelope, chromatin, and soluble components by sequential extractions...
  62. ncbi Identification of a candidate regulatory region in the human CD8 gene complex by colocalization of DNase I hypersensitive sites and matrix attachment regions which bind SATB1 and GATA-3
    Lynda J Kieffer
    Department of Laboratory Medicine and Department of Genetics and Section of Immunobiology, Yale University, New Haven, CT 06520, USA
    J Immunol 168:3915-22. 2002
    To locate elements regulating the human CD8 gene complex, we mapped nuclear matrix attachment regions (MARs) and DNase I hypersensitive (HS) sites over a 100-kb region that included the CD8B gene, the intergenic region, and the CD8A gene...
  63. ncbi A possible role of cholesterol-sphingomyelin/phosphatidylcholine in nuclear matrix during rat liver regeneration
    Elisabetta Albi
    Department of Biochemical Sciences and Molecular Biotechnology, Physiopathology, Policlinico Monteluce, 06100 Perugia, Italy
    J Hepatol 38:623-8. 2003
    ..The aim of the present work is to clarify if chromatin lipids may derive or not from nuclear matrix and if they have different roles.
  64. ncbi DNA binding and gene activation properties of the Nmp4 nuclear matrix transcription factors
    Kitti Torrungruang
    Department of Periodontics, Indiana University School of Dentistry, Indianapolis, Indiana 46202, USA
    J Biol Chem 277:16153-9. 2002
    Splice variants of the Nmp4 gene include nuclear matrix transcription factors that regulate the type I collagen alpha1(I) polypeptide chain (COL1A1) promoter and several matrix metalloproteinase (MMP) genes...
  65. ncbi Nuclear matrix proteins as biomarkers in prostate cancer
    Eddy S Leman
    Department of Urology, Cellular and Molecular Pathology Graduate Program and University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pennsylvania 15232, USA
    J Cell Biochem 86:213-23. 2002
    The nuclear matrix (NM) is the structural framework of the nucleus that consists of the peripheral lamins and pore complexes, an internal ribonucleic protein network, and residual nucleoli...
  66. ncbi CK2 phosphorylation weakens 90 kDa MFP1 association to the nuclear matrix in Allium cepa
    Rafael Samaniego
    Nuclear Matrix Laboratory, Centro de Investigaciones Biologicas, CSIC, 28040 Madrid, Spain
    J Exp Bot 57:113-24. 2006
    ..plant coiled-coil protein located on the stroma side of the chloroplast thylakoids, as well as in the nuclear matrix. It displays species-specific variability in the number of genes, proteins, and expression...
  67. doi Regulation of rat haptoglobin gene expression is coordinated by the nuclear matrix
    Melita Vidakovic
    Department of Molecular Biology, Institute for Biological Research, University of Belgrade, Bulevar Despota Stefana 142, 11060 Belgrade, Serbia
    J Cell Biochem 107:1205-21. 2009
    ..The results show that the lamin components of the nuclear matrix form a network of functional, dynamic protein-protein and protein-Hp-S/MAR associations with multiple partners,..
  68. ncbi SUMO-1 modification of the acute promyelocytic leukaemia protein PML: implications for nuclear localisation
    E Duprez
    Molecular Structure and Function Laboratory, Imperial Cancer Research Fund, London WC2A 3PX, UK
    J Cell Sci 112:381-93. 1999
    ..Our results provide significant insights into the role of SUMO-1 modification of PML in both normal cells and the APL disease state...
  69. ncbi The PML gene encodes a phosphoprotein associated with the nuclear matrix
    K S Chang
    Department of Hematology, University of Texas M D Anderson Cancer Center, Houston 77030, USA
    Blood 85:3646-53. 1995
    ..In K562 and NIH/3T3 cells transfected with a PML expression plasmid, we found PML to be associated with the nuclear matrix. Our results also showed that PML is a phosphorprotein...
  70. ncbi Co-localization of poly(ADPR)polymerase 1 (PARP-1) poly(ADPR)polymerase 2 (PARP-2) and related proteins in rat testis nuclear matrix defined by chemical cross-linking
    Filomena Tramontano
    Department of Biological Chemistry, University Federico II of Naples, Via Mezzocannone 16, 80134 Napoli, Italy
    J Cell Biochem 94:58-66. 2005
    ..Moreover, the association of pADPR and PARP-1 with the nuclear matrix (NM) has been reported, based on the poly(ADP-ribosyl)ation of nuclear matrix proteins (NMPs)...
  71. ncbi The fate of the nuclear matrix-associated-region-binding protein SATB1 during apoptosis
    J Gotzmann
    Institute of Tumor Biology Cancer Research, University of Vienna, A 1090 Vienna, Austria
    Cell Death Differ 7:425-38. 2000
    ..protein 1 (SATB1), predominantly expressed in thymocytes, was identified as a component of the nuclear matrix protein fraction...
  72. pmc Interaction of the tau2 transcriptional activation domain of glucocorticoid receptor with a novel steroid receptor coactivator, Hic-5, which localizes to both focal adhesions and the nuclear matrix
    L Yang
    Department of Pathology and Department of Biochemistry and Molecular Biology, University of Southern California, Los Angeles, California 90089, USA
    Mol Biol Cell 11:2007-18. 2000
    ..identified Hic-5 as a protein that interacts with a region of the glucocorticoid receptor that includes a nuclear matrix-targeting signal and the tau2 transcriptional activation domain...
  73. pmc Subnuclear proteomics in colorectal cancer: identification of proteins enriched in the nuclear matrix fraction and regulation in adenoma to carcinoma progression
    Jakob Albrethsen
    OncoProteomics Laboratory, Department of Medical Oncology, VU University Medical Center VUmc Cancer Center Amsterdam, The Netherlands
    Mol Cell Proteomics 9:988-1005. 2010
    ..in subnuclear domains in colorectal cancer tissues and apply this work flow to a comparative analysis of the nuclear matrix fraction in colorectal adenoma and carcinoma tissue samples...
  74. ncbi The effect of MAR elements on variation in spatial and temporal regulation of transgene expression
    W Van Leeuwen
    Laboratory of Plant Physiology, Wageningen University and Research Centre, Netherlands
    Plant Mol Biol 47:543-54. 2001
    ..The potential effects of MAR elements on the variability of transgene expression and the relation to the stability of the (trans)gene product are discussed...
  75. ncbi Association of the telomere-telomere-binding protein complex of hypotrichous ciliates with the nuclear matrix and dissociation during replication
    J Postberg
    Institute for Cell Biology, University Witten Herdecke, Stockumer Str 10, D 58448 Witten, Germany
    J Cell Sci 114:1861-6. 2001
    Telomeric interactions with the nuclear matrix have been described in a variety of eukaryotic cells and seem to be essential for specific nuclear localization...
  76. ncbi A novel alpha-helical protein, specific to and highly conserved in plants, is associated with the nuclear matrix fraction
    Annkatrin Rose
    Plant Biotechnology Center and Department of Plant Biology, Ohio State University, Columbus, OH 43210, USA
    J Exp Bot 54:1133-41. 2003
    A cDNA for a novel plant protein was isolated from tomato. Nuclear Matrix Protein 1 (NMP1) is a ubiquitously expressed 36 kDa protein, which has no homologues in animals and fungi, but is highly conserved among flowering and non-flowering ..
  77. ncbi Cloning and characterization of a novel zinc finger protein that associates with nuclear matrix
    J Y Lee
    Division of Chemotherapy, Chiba Cancer Center Research Institute, Japan
    DNA Cell Biol 17:849-58. 1998
    ..of 75 kDa (p75), which was distinct from Vn, existed in the nuclear fraction, and, more specifically, in the nuclear matrix fraction, of NIH3T3 cells...
  78. ncbi Identification of proteins immunologically related to vertebrate lamins in the nuclear matrix of the myxomycete Physarum polycephalum
    S Lang
    Department of Microbiology, University of Innsbruck Medical School, Austria
    Eur J Cell Biol 61:177-83. 1993
    Lamin-like proteins have been identified as components of the nuclear matrix of the myxomycete Physarum polycephalum...
  79. ncbi Chronic cyclophosphamide exposure alters the profile of rat sperm nuclear matrix proteins
    Alexis M Codrington
    Department of Pharmacology, McGill University, Montreal, Quebec, Canada H3G 1Y6
    Biol Reprod 77:303-11. 2007
    ..Sperm DNA is organized by the nuclear matrix into loop-domains in a sequence-specific manner...
  80. doi Metabolic stabilization of p53 by FE65 in the nuclear matrix of osmotically stressed cells
    Tadashi Nakaya
    Laboratory of Neuroscience, Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan
    FEBS J 276:6364-74. 2009
    ..Within the nucleus, FE65 formed a patched structure at the nuclear matrix, which facilitated the induction of gammaH2AX [Nakaya T, Kawai T & Suzuki T (2008) J Biol Chem283, 19119-..
  81. ncbi The conserved domain CR2 of Epstein-Barr virus nuclear antigen leader protein is responsible not only for nuclear matrix association but also for nuclear localization
    A Yokoyama
    Department of Tumor Virology, Medical Research Institute, Tokyo Medical and Dental University, Tokyo, 1-5-45, Yushima, Bunkyo-ku, 113-8510, Japan
    Virology 279:401-13. 2001
    There is a growing body of evidence for the importance of the nuclear matrix in various nuclear events including gene expression and DNA replication...
  82. ncbi Hairless is translocated to the nucleus via a novel bipartite nuclear localization signal and is associated with the nuclear matrix
    K Djabali
    Departments of Dermatology and Genetics and Development, Columbia University, College of Physicians and Surgeons, New York, New York, USA
    J Cell Sci 114:367-76. 2001
    ..Furthermore, nuclear fractionation analysis revealed that the hr protein is associated with components of the nuclear matrix.
  83. pmc Nuclear matrix association: switching to the invasive cytotrophoblast
    K J Drennan
    Department of Obstetrics and Gynecology, Wayne State University, 253 C S Mott Center, 275 E Hancock St, Detroit, MI 48201, USA
    Placenta 31:365-72. 2010
    ..well characterized invasive and non-invasive behavior, and to correlate the activity of the transcriptome with nuclear matrix attachment and cell phenotype. Comparison of the invasive to non-invasive HTR transcriptomes was unremarkable...
  84. ncbi Acute-phase-dependent binding affinity of C/EBPbeta from the nuclear extract and nuclear matrix towards the hormone response element of the alpha2-macroglobulin gene in rat hepatocytes
    D Bogojevic
    Molecular Biology Laboratory, Institute for Biological Research, 29 Novembra 142, 11000 Belgrade, Serbia and Montenegro
    Gen Physiol Biophys 22:279-85. 2003
    Interactions of nuclear extract and nuclear matrix proteins from rat hepatocytes with the hormone response element of the alpha2-macroglobulin gene were studied...
  85. ncbi Nuclear matrix localization of high mobility group protein I(Y) in a transgenic mouse model for prostate cancer
    Eddy S Leman
    Department of Urology, University of Pittsburgh School of Medicine, Pennsylvania 15232, USA
    J Cell Biochem 88:599-608. 2003
    Nuclear shape and the underlying nuclear structure, the nuclear matrix in cancer cells. Since the NM composition is considered to maintain nuclear shape and architecture, nuclear matrix proteins (NMPs) may be involved in transformation...
  86. pmc 1alpha,25-dihydroxy vitamin D(3) induces nuclear matrix association of the 1alpha,25-dihydroxy vitamin D(3) receptor in osteoblasts independently of its ability to bind DNA
    Gloria Arriagada
    Facultad de Ciencias Biologicas, Departamento de Bioquimica y Biologia Molecular, Universidad de Concepcion, Concepcion, Chile
    J Cell Physiol 222:336-46. 2010
    ..Here, we demonstrate that in osteoblastic cells, the VDR binds to the nuclear matrix in a vitamin D(3)-dependent manner...
  87. ncbi Nuclear envelope and nuclear matrix: interactions and dynamics
    S Vlcek
    Department of Biochemistry and Molecular Cell Biology, Vienna Biocenter, University of Vienna, Austria
    Cell Mol Life Sci 58:1758-65. 2001
    ..filaments that connect to nuclear pore complexes, are proposed to be major structural elements of the internal nuclear matrix. We describe the structural links between the peripheral lamina and the internal nuclear matrix that are ..
  88. ncbi Human satellite 3 (HS3) binding protein from the nuclear matrix: isolation and binding properties
    O Podgornaya
    Institute of Cytology, Russian Academy of Sciences, St Petersburg 164064, Russia
    Biochim Biophys Acta 1497:204-14. 2000
    Satellite DNA (satDNA) is the main component of residual DNA in nuclear matrix (NM) preparations. Gel mobility shift assay (GMSA) revealed specific human satellite 3 (HS3) binding activity in NM extracts...
  89. ncbi Involvement of the nuclear matrix in the control of skeletal genes: the NMP1 (YY1), NMP2 (Cbfa1), and NMP4 (Nmp4/CIZ) transcription factors
    J P Bidwell
    Department of Anatomy and Cell Biology, Indiana University School of Dentistry, Indianapolis 46202, USA
    Crit Rev Eukaryot Gene Expr 11:279-97. 2001
    The functional role of the osteoblast nuclear matrix has been a matter of supposition...
  90. ncbi Association of topoisomerase II with the hepatoma cell nuclear matrix: the role of intermolecular disulfide bond formation
    S H Kaufmann
    Department of Pharmacology, Johns Hopkins University School of Medicine, Johns Hopkins Hospital, Baltimore, Maryland 21205
    Exp Cell Res 192:511-23. 1991
    ..in conflicting data regarding the recovery of the nuclear enzymes topoisomerase (topo) II and topo I in the nuclear matrix fraction...
  91. pmc Characterization of SAF-A, a novel nuclear DNA binding protein from HeLa cells with high affinity for nuclear matrix/scaffold attachment DNA elements
    H Romig
    Department of Biology, University of Konstanz, Germany
    EMBO J 11:3431-40. 1992
    ..SAF-A is an abundant nuclear protein and a constituent of the nuclear matrix and scaffold...
  92. ncbi Recruitment of RNA polymerase II in the Ifng gene promoter correlates with the nuclear matrix association in activated T helper cells
    Elvira R Eivazova
    Vanderbilt University School of Medicine, Department of Medicine, Nashville, TN 37232, USA
    J Mol Biol 371:317-22. 2007
    ..However, the interaction of the Ifng gene promoter with the nuclear matrix occurred differentially in a lineage-specific manner...
  93. pmc Induction of transcription within chromosomal DNA loops flanked by MAR elements causes an association of loop DNA with the nuclear matrix
    Olga V Iarovaia
    Laboratory of Structural and Functional Organization of Chromosomes, Institute of Gene Biology RAS, Vavilov Street 34 5, 117984 Moscow, Russia
    Nucleic Acids Res 33:4157-63. 2005
    ..It is likely that the loop observed was attached to the nuclear matrix via MAR elements present at the flanks of the area under study...
  94. ncbi Distinct LIM domains of Hic-5/ARA55 are required for nuclear matrix targeting and glucocorticoid receptor binding and coactivation
    Jennifer Guerrero-Santoro
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA
    J Cell Biochem 92:810-9. 2004
    ..LIM4 also functions as a nuclear matrix targeting sequence (NMTS) for Hic-5/ARA55, as it is both necessary and sufficient to target a heterologous ..
  95. ncbi Natural ageing in the rat liver correlates with progressive stabilisation of DNA-nuclear matrix interactions and withdrawal of genes from the nuclear substructure
    Apolinar Maya-Mendoza
    Laboratorio de Biologia Molecular, Facultad de Medicina, Universidad Autonoma del Estado de Mexico, Toluca, Edo
    Mech Ageing Dev 126:767-82. 2005
    In the interphase nucleus, the DNA of higher eukaryotes is organised in supercoiled loops anchored to a nuclear matrix (NM). Replication, transcription and splicing seem to occur at macromolecular complexes organised upon the NM...
  96. ncbi Differential nuclear localization and nuclear matrix association of the splicing factors PSF and PTB
    M Meissner
    Institute of Tumor Biology Cancer Research, University of Vienna, A 1090 Vienna, Austria
    J Cell Biochem 76:559-66. 2000
    A monoclonal antibody raised against nuclear matrix proteins detected a protein of basic pI in human nuclear matrix protein samples of various cellular origin...
  97. doi Cell-type-specific organization of nuclear DNA into structural looped domains
    Claudia Trevilla-García
    Facultad de Medicina, Universidad Autonoma del Estado de Mexico, Toluca, Mexico, Mexico
    J Cell Biochem 112:531-40. 2011
    ..metazoan cells the DNA is organized in supercoiled loops anchored to a proteinaceous substructure known as the nuclear matrix (NM)...
  98. ncbi Positional mapping of specific DNA sequences relative to the nuclear substructure by direct polymerase chain reaction on nuclear matrix-bound templates
    Apolinar Maya-Mendoza
    Laboratorio de Biologia Molecular, Facultad de Medicina, Universidad Autonoma del Estado de Mexico, Apartado Postal 428, C P 50000, Toluca, Edo Mex, Mexico
    Anal Biochem 313:196-207. 2003
    ..eukaryotes is organized in supercoiled loops anchored to a proteinaceous substructure commonly known as the nuclear matrix. Current evidence suggests that important processes of nuclear physiology, such as replication, transcription, ..
  99. ncbi Cell cycle dependent regulation of protein kinase CK2 signaling to the nuclear matrix
    Huamin Wang
    Cellular and Molecular Biochemistry Research Laboratory 151, Minneapolis Veterans Affairs Medical Center and Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, Minnesota 55417, USA
    J Cell Biochem 88:812-22. 2003
    ..b>Nuclear matrix is a key locus for CK2 signaling in the nucleus...
  100. ncbi RIF-1, a novel nuclear receptor corepressor that associates with the nuclear matrix
    Hui Joyce Li
    Molecular Cardiology Research Institute, New England Medical Center, Tufts University School of Medicine, Boston, Massachusetts 02111, USA
    J Cell Biochem 102:1021-35. 2007
    ..RIF1 is localized exclusively in the cell nucleus and specifically to the nuclear matrix. Mutation of the nuclear localization signal abolishes this nuclear localization and causes RIF1 to appear in ..
  101. ncbi The nuclear matrix and chromosomal DNA loops: is their any correlation between partitioning of the genome into loops and functional domains?
    S V Razin
    Laboratory of Structural and Functional Organization of Chromosomes, Institute of Gene Biology of the Russian Academy of Sciences, Moscow
    Cell Mol Biol Lett 6:59-69. 2001
    ..Although the DNA loops attached to the nuclear matrix (chromosomal scaffold) are frequently considered as independent functional domains, this supposition lack ..

Research Grants69

  1. Preclinical assay development of a novel Colon Cancer Marker (CCSA-4)
    JACK CLEMENS; Fiscal Year: 2007
    ..Onconome, Inc. has licensed these novel nuclear matrix proteins and is developing products for the detection of these biomarkers for research and clinical use...
  2. ISOLATION OF THE FANCONI ANEMIA NUCLEAR PROTEIN COMPLEX
    Gary Kupfer; Fiscal Year: 2003
    ..Within the nucleus the data reveal that these proteins localize to the DNA and nuclear matrix-containing fractions and have an appearance that parallels that of other nuclear matrix proteins...
  3. INTRACELLULAR MECHANISMS OF GLUCOCORTICOID ACTION
    Donald Defranco; Fiscal Year: 1999
    ..In ATP-depleted cells, GRs remain localized within the nucleus and, in fact, are associated with the nuclear matrix. The restoration of cellular ATP levels leads to the release of matrix-bound GR in vivo...
  4. Anaplasma regulation of host granulocyte function
    John Stephen Dumler; Fiscal Year: 2010
    ..reprograms the global neutrophil transcriptome by altering the epigenome through AnkA"s action on nuclear matrix, chromatin, and transcriptional apparatus recruitment. We propose the following aims: 1...
  5. Repair of oxidative damage in mammalian genomes
    Tapas K Hazra; Fiscal Year: 2010
    ..mutations in NEIL2-deficient cells, are consistent with this hypothesis, hnRNP-U, an abundant multifunctional nuclear matrix protein, has also been shown to regulate transcription. NEIL2 carries out a beta-delta-reaction like E...
  6. Repair of oxidative damage in mammalian genomes
    TAPAS HAZRA; Fiscal Year: 2009
    ..mutations in NEIL2-deficient cells, are consistent with this hypothesis, hnRNP-U, an abundant multifunctional nuclear matrix protein, has also been shown to regulate transcription. NEIL2 carries out a beta-delta-reaction like E...
  7. Repair of oxidative damage in mammalian genomes
    TAPAS HAZRA; Fiscal Year: 2007
    ..mutations in NEIL2-deficient cells, are consistent with this hypothesis, hnRNP-U, an abundant multifunctional nuclear matrix protein, has also been shown to regulate transcription. NEIL2 carries out a beta-delta-reaction like E...
  8. KSHV-Ori-Lyt-Dependent DNA Replication
    Yan Yuan; Fiscal Year: 2010
    ..Two cellular proteins that may involve origin DNA unwinding and nuclear matrix attachment will be further studied because these two events are believed to be antecedent steps before ..
  9. Proteomic Characterization of IC Bladder
    Pradeep Tyagi; Fiscal Year: 2006
    ..The nuclear matrix is the structural scaffolding of the cell nucleus and plays a central role in the regulation of important ..
  10. NUCLEAR MATRIX STRUCTURE AND DNA REPLICATION
    Ronald Berezney; Fiscal Year: 1993
    ..Our laboratory has been studying the nuclear matrix and its role in DNA replication for over 15 years...
  11. NUCLEAR PROTEIN CONTENT AND HEAT INDUCED CELL KILLING
    Joseph Roti Roti; Fiscal Year: 1999
    The objective of the proposed work is to test 2 hypotheses regarding proteins whose nuclear matrix (NM) association is modified by heat shock (i.e., HMNMP, hyperthermia modifiable NM proteins)...
  12. CHROMATIN-TELOMERE STRUCTURE AND AT GENOMIC SENSITIVITY
    TEJ PANDITA; Fiscal Year: 2005
    ..We demonstrated that altered telomere nuclear matrix interactions and nucleosomal periodicity observed in cells derived from individuals with ataxia telangiectasia ..
  13. ER Co-Repressor Function of SAFB in Breast Cancer
    Steffi Oesterreich; Fiscal Year: 2005
    The scaffold attachment factor/nuclear matrix protein SAFB maps to a locus with extremely high loss of heterozygosity in human breast cancer...
  14. ER Co-Repressor Function of SAFB in Breast Cancer
    Adrian Lee; Fiscal Year: 2006
    The scaffold attachment factor/nuclear matrix protein SAFB maps to a locus with extremely high loss of heterozygosity in human breast cancer...
  15. NUCLEAR MATRIX STRUCTURE AND DNA REPLICATION
    Ronald Berezney; Fiscal Year: 1992
    ..1) What are the cell cycle relationships of different replicational components associated with the nuclear matrix? Various properties of DNA polymerase alpha, DNA primase, diadenosine tetraphosphate (AP4A) binding sites, DNA ..