cell nucleus

Summary

Summary: Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)

Top Publications

  1. pmc Comprehensive mapping of long-range interactions reveals folding principles of the human genome
    Erez Lieberman-Aiden
    Broad Institute of Harvard and Massachusetts Institute of Technology MIT, MA 02139, USA
    Science 326:289-93. 2009
  2. ncbi The Wnt signaling pathway in development and disease
    Catriona Y Logan
    Department of Developmental Biology, Beckman Center, Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA
    Annu Rev Cell Dev Biol 20:781-810. 2004
  3. ncbi Capturing chromosome conformation
    Job Dekker
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    Science 295:1306-11. 2002
  4. ncbi Sensing DNA damage through ATRIP recognition of RPA-ssDNA complexes
    Lee Zou
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Science 300:1542-8. 2003
  5. ncbi Notch signaling: cell fate control and signal integration in development
    S Artavanis-Tsakonas
    Massachusetts General Hospital Cancer Center, Department of Cell Biology, Harvard Medical School, Building 149, 13th Street, Charlestown, MA 02129, USA
    Science 284:770-6. 1999
  6. doi Transcriptional paradigms in mammalian mitochondrial biogenesis and function
    Richard C Scarpulla
    Department of Cell and Molecular Biology, Northwestern Medical School, Chicago, Illinois 60611, USA
    Physiol Rev 88:611-38. 2008
  7. ncbi Molecular characterization of mitochondrial apoptosis-inducing factor
    S A Susin
    Centre National de la Recherche Scientifique, UPR 420, Villejuif, France
    Nature 397:441-6. 1999
  8. pmc Elucidation of a universal size-control mechanism in Drosophila and mammals
    Jixin Dong
    Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Cell 130:1120-33. 2007
  9. pmc Subdiffraction multicolor imaging of the nuclear periphery with 3D structured illumination microscopy
    Lothar Schermelleh
    Center for Integrated Protein Science, Department of Biology, Ludwig Maximilians University Munich, 82152 Planegg Martinsried, Germany
    Science 320:1332-6. 2008
  10. pmc A three-dimensional model of the yeast genome
    Zhijun Duan
    Institute for Stem Cell and Regenerative Medicine, University of Washington, Seattle, Washington 98195 8056, USA
    Nature 465:363-7. 2010

Detail Information

Publications338 found, 100 shown here

  1. pmc Comprehensive mapping of long-range interactions reveals folding principles of the human genome
    Erez Lieberman-Aiden
    Broad Institute of Harvard and Massachusetts Institute of Technology MIT, MA 02139, USA
    Science 326:289-93. 2009
    ..The fractal globule is distinct from the more commonly used globular equilibrium model. Our results demonstrate the power of Hi-C to map the dynamic conformations of whole genomes...
  2. ncbi The Wnt signaling pathway in development and disease
    Catriona Y Logan
    Department of Developmental Biology, Beckman Center, Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA
    Annu Rev Cell Dev Biol 20:781-810. 2004
    ..The next few years are likely to see novel therapeutic reagents aimed at controlling Wnt signaling in order to alleviate these conditions...
  3. ncbi Capturing chromosome conformation
    Job Dekker
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    Science 295:1306-11. 2002
    ..Furthermore, functionally distinct AT- and GC-rich domains were found to exhibit different conformations, and a population-average 3D model of chromosome III could be determined. Chromosome III emerges as a contorted ring...
  4. ncbi Sensing DNA damage through ATRIP recognition of RPA-ssDNA complexes
    Lee Zou
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Science 300:1542-8. 2003
    ..Our data suggest that RPA-coated ssDNA is the critical structure at sites of DNA damage that recruits the ATR-ATRIP complex and facilitates its recognition of substrates for phosphorylation and the initiation of checkpoint signaling...
  5. ncbi Notch signaling: cell fate control and signal integration in development
    S Artavanis-Tsakonas
    Massachusetts General Hospital Cancer Center, Department of Cell Biology, Harvard Medical School, Building 149, 13th Street, Charlestown, MA 02129, USA
    Science 284:770-6. 1999
    ..Notch activity affects the implementation of differentiation, proliferation, and apoptotic programs, providing a general developmental tool to influence organ formation and morphogenesis...
  6. doi Transcriptional paradigms in mammalian mitochondrial biogenesis and function
    Richard C Scarpulla
    Department of Cell and Molecular Biology, Northwestern Medical School, Chicago, Illinois 60611, USA
    Physiol Rev 88:611-38. 2008
    ..These transcriptional paradigms provide a basic framework for understanding the integration of mitochondrial biogenesis and function with signaling events that dictate cell- and tissue-specific energetic properties...
  7. ncbi Molecular characterization of mitochondrial apoptosis-inducing factor
    S A Susin
    Centre National de la Recherche Scientifique, UPR 420, Villejuif, France
    Nature 397:441-6. 1999
    ..These results indicate that AIF is a mitochondrial effector of apoptotic cell death...
  8. pmc Elucidation of a universal size-control mechanism in Drosophila and mammals
    Jixin Dong
    Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Cell 130:1120-33. 2007
    ..These results uncover a universal size-control mechanism in metazoan...
  9. pmc Subdiffraction multicolor imaging of the nuclear periphery with 3D structured illumination microscopy
    Lothar Schermelleh
    Center for Integrated Protein Science, Department of Biology, Ludwig Maximilians University Munich, 82152 Planegg Martinsried, Germany
    Science 320:1332-6. 2008
    ..Multicolor 3D-SIM opens new and facile possibilities to analyze subcellular structures beyond the diffraction limit of the emitted light...
  10. pmc A three-dimensional model of the yeast genome
    Zhijun Duan
    Institute for Stem Cell and Regenerative Medicine, University of Washington, Seattle, Washington 98195 8056, USA
    Nature 465:363-7. 2010
    ..Finally, we constructed a three-dimensional model of the yeast genome. Our findings provide a glimpse of the interface between the form and function of a eukaryotic genome...
  11. ncbi Mechanisms of TGF-beta signaling from cell membrane to the nucleus
    Yigong Shi
    Department of Molecular Biology, Princeton University, Washington Road, Princeton, NJ 08544, USA
    Cell 113:685-700. 2003
    ..This article reviews these latest advances and presents our current understanding on the mechanisms of TGF-beta signaling from cell membrane to the nucleus...
  12. ncbi Functional interaction of beta-catenin with the transcription factor LEF-1
    J Behrens
    Max Delbruck Centre for Molecular Medicine, Berlin, Germany
    Nature 382:638-42. 1996
    ..Thus beta-catenin regulates gene expression by direct interaction with transcription factors such as LEF-1, providing a molecular mechanism for the transmission of signals, from cell-adhesion components or wnt protein to the nucleus...
  13. ncbi The nuclear RNase III Drosha initiates microRNA processing
    Yoontae Lee
    Institute of Molecular Biology and Genetics and School of Biological Sciences, Seoul National University, Seoul 151 742, Korea
    Nature 425:415-9. 2003
    ..Thus, the two RNase III proteins, Drosha and Dicer, may collaborate in the stepwise processing of miRNAs, and have key roles in miRNA-mediated gene regulation in processes such as development and differentiation...
  14. pmc Exportin 5 is a RanGTP-dependent dsRNA-binding protein that mediates nuclear export of pre-miRNAs
    Markus T Bohnsack
    Zentrum fur Molekulare Biologie, Heidelberg, Germany
    RNA 10:185-91. 2004
    ..We further show that exportin 5 interacts with double-stranded RNA in a sequence-independent manner...
  15. ncbi Cellular motility driven by assembly and disassembly of actin filaments
    Thomas D Pollard
    Department of Cellular, Molecular, and Developmental Biology, Yale University, New Haven, CT 06520, USA
    Cell 112:453-65. 2003
    ..Profilin catalyzes the exchange of ADP for ATP, refilling the pool of ATP-actin monomers bound to profilin, ready for elongation...
  16. ncbi Mediation of poly(ADP-ribose) polymerase-1-dependent cell death by apoptosis-inducing factor
    Seong Woon Yu
    Department of Neurology and Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA
    Science 297:259-63. 2002
    ..These data support a model in which PARP-1 activation signals AIF release from mitochondria, resulting in a caspase-independent pathway of programmed cell death...
  17. pmc Flexible use of nuclear import pathways by HIV-1
    Kyeongeun Lee
    HIV Drug Resistance Program, National Cancer Institute, Frederick, MD 21702, USA
    Cell Host Microbe 7:221-33. 2010
    ..These findings reveal a remarkable flexibility in HIV-1 nuclear transport and highlight a single residue in CA as essential in regulating interactions with NUPs...
  18. pmc Coupling of the nucleus and cytoplasm: role of the LINC complex
    Melissa Crisp
    Department of Anatomy and Cell Biology, University of Florida, Gainesville, FL 32610
    J Cell Biol 172:41-53. 2006
    ..2005), support a model in which Sun proteins tether nesprins in the ONM via interactions spanning the PNS. In this way, Sun proteins and nesprins form a complex that links the nucleoskeleton and cytoskeleton (the LINC complex)...
  19. doi Coordination of gene expression between organellar and nuclear genomes
    Jesse D Woodson
    Plant Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, California 92037, USA
    Nat Rev Genet 9:383-95. 2008
    ..Here we review recent advances in elucidating the intracellular signalling pathways that coordinate gene expression between organelles and the nucleus, with a focus on photosynthetic plants...
  20. ncbi IkappaB kinase promotes tumorigenesis through inhibition of forkhead FOXO3a
    Mickey C T Hu
    Department of Molecular and Cellular Oncology, The University of Texas, Houston, TX 77030, USA
    Cell 117:225-37. 2004
    ..These results suggest the negative regulation of FOXO factors by IKK as a key mechanism for promoting cell growth and tumorigenesis...
  21. ncbi Dual regulation of Snail by GSK-3beta-mediated phosphorylation in control of epithelial-mesenchymal transition
    Binhua P Zhou
    Department of Molecular and Cellular Oncology, Breast Cancer Basic Research Program, The University of Texas M D Anderson Cancer Center, Houston, Texas 77030, USA
    Nat Cell Biol 6:931-40. 2004
    ..Furthermore, inhibition of GSK-3beta results in the upregulation of Snail and downregulation of E-cadherin in vivo. Thus, Snail and GSK-3beta together function as a molecular switch for many signalling pathways that lead to EMT...
  22. doi Reconstruction of zebrafish early embryonic development by scanned light sheet microscopy
    Philipp J Keller
    Cell Biology and Biophysics Unit, European Molecular Biology Laboratory EMBL, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    Science 322:1065-9. 2008
    ..We further derive a model of germ layer formation and show that the mesendoderm forms from one-third of the embryo's cells in a single event. Our digital embryos, with 55 million nucleus entries, are provided as a resource...
  23. ncbi Exporting RNA from the nucleus to the cytoplasm
    Alwin Köhler
    Biochemie Zentrum der Universitat Heidelberg, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany
    Nat Rev Mol Cell Biol 8:761-73. 2007
    ..Export of mRNAs is unique as it is extensively coupled to transcription (in yeast) and splicing (in metazoa). Understanding the mechanisms that connect RNP formation with export is a major challenge in the field...
  24. ncbi Nuclear export of microRNA precursors
    Elsebet Lund
    Department of Biomolecular Chemistry, University of Wisconsin Medical School, Madison, WI 53706, USA
    Science 303:95-8. 2004
    ..Thus, Exp5 is key to miRNA biogenesis and may help coordinate nuclear and cytoplasmic processing steps...
  25. ncbi Transcriptional regulatory circuits controlling mitochondrial biogenesis and function
    Daniel P Kelly
    Center for Cardiovascula Research, Departments of Medicine, Molecular Biology and Pharmacology, and Pediatrics, Washington University School of Medicine, St Louis, Missouri 63119, USA
    Genes Dev 18:357-68. 2004
  26. pmc Interactions between nuclei and the cytoskeleton are mediated by SUN-KASH nuclear-envelope bridges
    Daniel A Starr
    Department of Molecular and Cellular Biology, University of California, Davis, California 95616, USA
    Annu Rev Cell Dev Biol 26:421-44. 2010
    ..Knockouts of SUN or KASH proteins disrupt neurological and muscular development in mice. Defects in SUN and KASH proteins have been linked to human diseases including muscular dystrophy, ataxia, progeria, lissencephaly, and cancer...
  27. ncbi Regulation of the Caenorhabditis elegans longevity protein DAF-16 by insulin/IGF-1 and germline signaling
    K Lin
    Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, California, USA
    Nat Genet 28:139-45. 2001
    ..Together these findings reveal unexpected complexity in the DAF-16-dependent pathways that regulate aging...
  28. ncbi Formation of neuronal intranuclear inclusions underlies the neurological dysfunction in mice transgenic for the HD mutation
    S W Davies
    Department of Anatomy and Developmental Biology, University College London, United Kingdom
    Cell 90:537-48. 1997
    ..The appearance in transgenic mice of these inclusions, followed by characteristic morphological change within neuronal nuclei, is strikingly similar to nuclear abnormalities observed in biopsy material from HD patients...
  29. ncbi Dynamic genome architecture in the nuclear space: regulation of gene expression in three dimensions
    Christian Lanctôt
    Department Biologie II, Ludwig Maximilians Universitat, Grosshadernerstr 2, Planegg Martinsried, Germany
    Nat Rev Genet 8:104-15. 2007
    ..Understanding how the dynamic nature of the positioning of genetic material in the nuclear space and the higher-order architecture of the nucleus are integrated is therefore essential to our overall understanding of gene regulation...
  30. ncbi Homeodomain-interacting protein kinase-2 phosphorylates p53 at Ser 46 and mediates apoptosis
    Gabriella D'Orazi
    Molecular Oncogenesis Laboratory, Regina Elena Cancer Institute, Via delle Messi d Oro 156, 00158 Rome, Italy
    Nat Cell Biol 4:11-9. 2002
    ..These data define a new functional interaction between p53 and HIPK2 that results in the targeted subcellular localization of p53 and initiation of apoptosis...
  31. pmc Nuclear lamins
    Thomas Dechat
    Department of Cell and Molecular Biology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois 60611, USA
    Cold Spring Harb Perspect Biol 2:a000547. 2010
    ..They may also regulate chromatin functions by interacting with factors that epigenetically modify the chromatin or directly regulate replication or transcription...
  32. pmc The nuclear pore complex and nuclear transport
    Susan R Wente
    Department of Cell and Developmental Biology, Vanderbilt University Medical Center, Nashville, Tennessee 37232, USA
    Cold Spring Harb Perspect Biol 2:a000562. 2010
    ..All these requirements demonstrate the complex design of the NPC and the integral role it plays in key cellular processes...
  33. ncbi Mitochondrial retrograde signaling
    Zhengchang Liu
    Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9148, USA
    Annu Rev Genet 40:159-85. 2006
    ..Here we review advances in the yeast retrograde signaling pathway and highlight its regulatory factors and regulatory mechanisms, its physiological functions, and its connection to nutrient sensing, TOR signaling, and aging...
  34. ncbi The genome of the diatom Thalassiosira pseudonana: ecology, evolution, and metabolism
    E Virginia Armbrust
    School of Oceanography, University of Washington, Seattle, WA 98195, USA
    Science 306:79-86. 2004
    ....
  35. ncbi Dynamic FoxO transcription factors
    Haojie Huang
    Cancer Center and Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, MN 55455, USA
    J Cell Sci 120:2479-87. 2007
    ..Thus, the potent functions of FoxO proteins are tightly controlled by complex signaling pathways under physiological conditions; dysregulation of these proteins may ultimately lead to disease such as cancer...
  36. pmc Phosphorylation of beta-catenin by AKT promotes beta-catenin transcriptional activity
    Dexing Fang
    Brain Tumor Center and Department of Neuro Oncology, The University of Texas M D Anderson Cancer Center, Houston, Texas 77030, USA
    J Biol Chem 282:11221-9. 2007
    ..Phosphorylation of beta-catenin by AKT increases its transcriptional activity and promotes tumor cell invasion, indicating that AKT-dependent regulation of beta-catenin plays a critical role in tumor invasion and development...
  37. pmc Nuclear trafficking of the epidermal growth factor receptor family membrane proteins
    Y N Wang
    Department of Molecular and Cellular Oncology, University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA
    Oncogene 29:3997-4006. 2010
    ..Understanding the trafficking mechanisms as to how RTKs are transported from the cell surface to the nucleus will significantly contribute to understanding the functions of the nuclear RTKs...
  38. ncbi Post-translational modifications regulating the activity and function of the nuclear factor kappa B pathway
    N D Perkins
    Division of Gene Regulation and Expression, University of Dundee, Dundee, Scotland, UK
    Oncogene 25:6717-30. 2006
    ....
  39. pmc Interactions of mitochondrial and nuclear genes that affect male gametophyte development
    Maureen R Hanson
    Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14853 0001, USA
    Plant Cell 16:S154-69. 2004
  40. ncbi Driving ribosome assembly
    Dieter Kressler
    Biochemie Zentrum der Universitat Heidelberg, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany
    Biochim Biophys Acta 1803:673-83. 2010
    ..This review summarizes our current knowledge on eukaryotic ribosome assembly, with particular focus on the molecular role of the involved energy-consuming enzymes...
  41. ncbi Duration of nuclear NF-kappaB action regulated by reversible acetylation
    - Chen Lf
    Gladstone Institute of Virology and Immunology, Department of Medicine, University of California, San Francisco, CA 94141, USA
    Science 293:1653-7. 2001
    ....
  42. pmc An architectural role for a nuclear noncoding RNA: NEAT1 RNA is essential for the structure of paraspeckles
    Christine M Clemson
    University of Massachusetts Medical Center, 55 Lake Avenue North, Worcester, MA 01655, USA
    Mol Cell 33:717-26. 2009
    ..Collectively, results demonstrate that NEAT1 functions as an essential structural determinant of paraspeckles, providing a precedent for a ncRNA as the foundation of a nuclear domain...
  43. ncbi Endosymbiotic gene transfer: organelle genomes forge eukaryotic chromosomes
    Jeremy N Timmis
    School of Molecular and Biomedical Science, The University of Adelaide, South Australia 5005, Australia
    Nat Rev Genet 5:123-35. 2004
  44. ncbi Viable offspring derived from fetal and adult mammalian cells
    I Wilmut
    Roslin Institute Edinburgh, Roslin, Midlothian, UK
    Nature 385:810-3. 1997
    ....
  45. pmc Broadly sampled multigene analyses yield a well-resolved eukaryotic tree of life
    Laura Wegener Parfrey
    Program in Organismic and Evolutionary Biology, University of Massachusetts, 611 North Pleasant Street, Amherst, MA 01003, USA
    Syst Biol 59:518-33. 2010
    ....
  46. ncbi A hexanucleotide element directs microRNA nuclear import
    Hun Way Hwang
    Program in Human Genetics and Molecular Biology, McKusick Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Science 315:97-100. 2007
    ..Our results indicate that miRNAs sharing common 5' sequences, considered to be largely redundant, might have distinct functions because of the influence of cis-acting regulatory motifs...
  47. ncbi Genomic footprints of a cryptic plastid endosymbiosis in diatoms
    Ahmed Moustafa
    Interdisciplinary Program in Genetics, University of Iowa, Iowa City, IA 52242, USA
    Science 324:1724-6. 2009
    ..Chromalveolates appear to have recruited genes from the two major existing algal groups to forge a highly successful, species-rich protist lineage...
  48. ncbi NF-kappa B controls expression of inhibitor I kappa B alpha: evidence for an inducible autoregulatory pathway
    S C Sun
    Gladstone Institute of Virology and Immunology, University of California, San Francisco
    Science 259:1912-5. 1993
    ..Together, these results show that NF-kappa B controls the expression of I kappa B alpha by means of an inducible autoregulatory pathway...
  49. pmc Piecemeal microautophagy of nucleus in Saccharomyces cerevisiae
    Paul Roberts
    Department of Biology, University of Rochester, Rochester, New York 14627, USA
    Mol Biol Cell 14:129-41. 2003
    ..These results introduce a novel mode of selective microautophagy that targets nonessential components of the yeast nucleus for degradation and recycling in the vacuole...
  50. ncbi Aggregation of huntingtin in neuronal intranuclear inclusions and dystrophic neurites in brain
    M Difiglia
    Department of Neurology, Massachusetts General Hospital, Boston, MA 02114, USA
    Science 277:1990-3. 1997
    ..Ubiquitin was also found in NIIs and DNs, which suggests that abnormal huntingtin is targeted for proteolysis but is resistant to removal. The aggregation of mutant huntingtin may be part of the pathogenic mechanism in HD...
  51. pmc Chromosome territories
    Thomas Cremer
    Biozentrum, Department of Biology II, Ludwig Maximilians University, Grosshadernerstrasse 2, 82152 Martinsried, Germany
    Cold Spring Harb Perspect Biol 2:a003889. 2010
    ..The article concludes with a discussion of open questions and new experimental strategies to answer them...
  52. pmc Bringing KASH under the SUN: the many faces of nucleo-cytoskeletal connections
    David Razafsky
    Department of Ophthalmology and Visual Sciences, Washington University School of Medicine, St Louis, MO 63110, USA
    J Cell Biol 186:461-72. 2009
    ..Moreover, we discuss the function of these complexes in nuclear, centrosomal, and chromosome dynamics, and their connection to human disease...
  53. pmc Nuclear neighborhoods and gene expression
    Rui Zhao
    Molecular and Cellular Biology Program, Stony Brook University, Stony Brook, NY 11794, USA
    Curr Opin Genet Dev 19:172-9. 2009
    ..We are still at a very early, yet exciting stage in our journey toward deciphering the mechanism(s) that govern(s) the permissiveness of gene expression/repression within different nuclear neighborhoods...
  54. pmc Ubiquitination regulates PTEN nuclear import and tumor suppression
    Lloyd C Trotman
    Cancer Biology and Genetics Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    Cell 128:141-56. 2007
    ....
  55. pmc A mechanism of repression of TGFbeta/ Smad signaling by oncogenic Ras
    M Kretzschmar
    Cell Biology Program and Howard Hughes Medical Institute, Memorial Sloan Kettering Cancer Center, New York, New York 10021 USA
    Genes Dev 13:804-16. 1999
    ..Our results suggest a mechanism for the counterbalanced regulation of Smad2/Smad3 by TGFbeta and Ras signals in normal cells, and for the silencing of antimitogenic TGFbeta functions by hyperactive Ras in cancer cells...
  56. pmc Exportin-5 mediates the nuclear export of pre-microRNAs and short hairpin RNAs
    Rui Yi
    Howard Hughes Medical Institute, Duke University Medical Center, Durham, NC 27710, USA
    Genes Dev 17:3011-6. 2003
    ..Together, these findings define an additional cellular cofactor required for miRNA biogenesis and function...
  57. pmc Paraspeckles: nuclear bodies built on long noncoding RNA
    Charles S Bond
    School of Biomedical, Biomolecular, and Chemical Sciences, University of Western Australia, Crawley, Western Australia 6009, Australia
    J Cell Biol 186:637-44. 2009
    ..Given the large numbers of long noncoding transcripts currently being discovered through whole transcriptome analysis, paraspeckles may be a paradigm for a class of subnuclear bodies formed around long noncoding RNA...
  58. pmc Acetylation of RelA at discrete sites regulates distinct nuclear functions of NF-kappaB
    Lin Feng Chen
    Gladstone Institute of Virology and Immunology, University of California at San Francisco, San Francisco, CA 94141, USA
    EMBO J 21:6539-48. 2002
    ..Together, these findings highlight how site-specific acetylation of RelA differentially regulates distinct biological activities of the NF-kappaB transcription factor complex...
  59. pmc Plasmodium falciparum heterochromatin protein 1 binds to tri-methylated histone 3 lysine 9 and is linked to mutually exclusive expression of var genes
    Karla Pérez-Toledo
    Departamento de Biomedicina Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional IPN, Apartado Postal 14 740, 07360, Mexico D F, Mexico
    Nucleic Acids Res 37:2596-606. 2009
    ..This is the first report implicating an HP1 protein in the control of antigenic variation of a protozoan parasite...
  60. ncbi The layered structure of human mitochondrial DNA nucleoids
    Daniel F Bogenhagen
    Department of Pharmacological Sciences, State University of New York at Stony Brook, Stony Brook, New York 11794 8651, USA
    J Biol Chem 283:3665-75. 2008
    ..These results are discussed in relation to a model for a layered structure of mtDNA nucleoids in which replication and transcription occur in the central core, whereas translation and complex assembly may occur in the peripheral region...
  61. pmc Molecular poltergeists: mitochondrial DNA copies (numts) in sequenced nuclear genomes
    Einat Hazkani-Covo
    National Evolutionary Synthesis Center, Durham, North Carolina, USA
    PLoS Genet 6:e1000834. 2010
    ..Polymorphic numts in humans link maternally inherited mitochondrial genotypes to nuclear DNA haplotypes during the past, offering new opportunities to associate nuclear markers with mitochondrial markers back in time...
  62. pmc Tumor suppressor p53 binding protein 1 (53BP1) is involved in DNA damage-signaling pathways
    I Rappold
    Division of Oncology Research, Mayo Clinic, Rochester, Minnesota 55905, USA
    J Cell Biol 153:613-20. 2001
    ..Third, 53BP1 is readily phosphorylated by ATM in vitro. Taken together, these results suggest that 53BP1 is an ATM substrate that is involved early in the DNA damage-signaling pathways in mammalian cells...
  63. ncbi IkappaB kinases phosphorylate NF-kappaB p65 subunit on serine 536 in the transactivation domain
    H Sakurai
    Discovery Research Laboratory, Tanabe Seiyaku Co, Ltd, 16 89 Kashima 3 chome, Yodogawa ku, Osaka 532 8505, Japan
    J Biol Chem 274:30353-6. 1999
    ..Furthermore, activation of IKKs by NF-kappaB-inducing kinase induced phosphorylation of p65 in vivo. Our finding, together with previous observations, suggests dual roles for IKK complex in the regulation of NF-kappaB.IkappaB complex...
  64. ncbi Diversity of LEF/TCF action in development and disease
    L Arce
    Department of Microbiology and Molecular Genetics, University of California, Irvine, CA 92697 4025, USA
    Oncogene 25:7492-504. 2006
    ..Recent work from numerous laboratories has highlighted how this complexity has important biological consequences in development and disease...
  65. pmc The obesity-associated FTO gene encodes a 2-oxoglutarate-dependent nucleic acid demethylase
    Thomas Gerken
    Chemistry Research Laboratory and Oxford Centre for Integrative Systems Biology, University of Oxford, 12 Mansfield Road, Oxford, Oxon OX1 3TA, UK
    Science 318:1469-72. 2007
    ..Studies can now be directed toward determining the physiologically relevant FTO substrate and how nucleic acid methylation status is linked to increased fat mass...
  66. pmc Both corepressor proteins SMRT and N-CoR exist in large protein complexes containing HDAC3
    J Li
    Department of Molecular and Cellular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
    EMBO J 19:4342-50. 2000
    ..These findings thus establish both SMRT and N-CoR complexes as bona fide HDAC-containing complexes and shed new light on the molecular pathways by which N-CoR and SMRT function in transcriptional repression...
  67. pmc Identification of a tumour suppressor network opposing nuclear Akt function
    Lloyd C Trotman
    Cancer Biology and Genetics Program, Memorial Sloan Kettering Cancer Center, Sloan Kettering Institute, 1275 York Avenue, New York, New York 10021, USA
    Nature 441:523-7. 2006
    ..Our results demonstrate that Pml orchestrates a nuclear tumour suppressor network for inactivation of nuclear pAkt, and thus highlight the importance of AKT compartmentalization in human cancer pathogenesis and treatment...
  68. doi Mechanotransduction at a distance: mechanically coupling the extracellular matrix with the nucleus
    Ning Wang
    Department of Mechanical Science and Engineering, University of Illinois at Urbana Champaign, 1206 West Green Street, Urbana, Illinois 61801, USA
    Nat Rev Mol Cell Biol 10:75-82. 2009
    ..Here, we explore the molecular mechanisms by which forces might act at a distance to induce mechanochemical conversion in the nucleus and alter gene activities...
  69. ncbi PSF and p54(nrb)/NonO--multi-functional nuclear proteins
    Yaron Shav-Tal
    Department of Molecular Cell Biology, The Weizmann Institute of Science, Rehovot, Israel
    FEBS Lett 531:109-14. 2002
    ....
  70. ncbi Transportin-SR2 imports HIV into the nucleus
    Frauke Christ
    Laboratory for Molecular Virology and Gene Therapy, KU Leuven and IRC, Kapucijnenvoer 33, B 3000 Leuven, Flanders, Belgium
    Curr Biol 18:1192-202. 2008
    ..This requires import of the preintegration complex (PIC) through the nuclear pore. Although many cellular and viral determinants have been proposed, the mechanism leading to nuclear import is not yet understood...
  71. pmc A yeast TDP-43 proteinopathy model: Exploring the molecular determinants of TDP-43 aggregation and cellular toxicity
    Brian S Johnson
    Department of Cell and Developmental Biology, University of Pennsylvania, Philadelphia, PA 19104, USA
    Proc Natl Acad Sci U S A 105:6439-44. 2008
    ..This work provides a mechanistic framework for investigating the toxicity of TDP-43 aggregation relevant to human disease and establishes a manipulable, high-throughput model for discovering potential therapeutic strategies...
  72. ncbi A critical role for histone H2AX in recruitment of repair factors to nuclear foci after DNA damage
    T T Paull
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892 0540, USA
    Curr Biol 10:886-95. 2000
    ..Recently it has been reported that a member of the histone H2A family, H2AX, becomes extensively phosphorylated within 1-3 minutes of DNA damage and forms foci at break sites...
  73. pmc Rad18 guides poleta to replication stalling sites through physical interaction and PCNA monoubiquitination
    Kenji Watanabe
    Institute of Molecular Embryology and Genetics, Kumamoto University, Kumamoto, Japan
    EMBO J 23:3886-96. 2004
    ..These results suggest that Rad18 is crucial for recruitment of poleta to the damaged site through protein-protein interaction and PCNA monoubiquitination...
  74. pmc Pathways of DNA double-strand break repair during the mammalian cell cycle
    Kai Rothkamm
    Fachrichtung Biophysik, Universitat des Saarlandes, D 66421 Homburg Saar, Germany
    Mol Cell Biol 23:5706-15. 2003
    ..These data provide the first evaluation of the cell cycle-specific contributions of NHEJ and HR to the repair of radiation-induced versus replication-associated DSBs...
  75. doi The SUN rises on meiotic chromosome dynamics
    Yasushi Hiraoka
    Graduate School of Frontier Biosciences, Osaka University, 1 3 Yamadaoka, Suita 565 0871, Japan
    Dev Cell 17:598-605. 2009
    ..During meiotic prophase, the apparent role of these SUN-domain proteins, together with their partner KASH-domain proteins, is to connect chromosomes through the intact nuclear envelope to force-generating mechanisms in the cytoplasm...
  76. pmc 3' end processing of a long nuclear-retained noncoding RNA yields a tRNA-like cytoplasmic RNA
    Jeremy E Wilusz
    Watson School of Biological Sciences, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
    Cell 135:919-32. 2008
    ..Our findings reveal a 3' end processing mechanism by which a single gene locus can yield both a stable nuclear-retained noncoding RNA with a short poly(A) tail-like moiety and a small tRNA-like cytoplasmic RNA...
  77. pmc Arabidopsis genomes uncoupled 5 (GUN5) mutant reveals the involvement of Mg-chelatase H subunit in plastid-to-nucleus signal transduction
    N Mochizuki
    Department of Botany, Graduate School of Science, Kyoto University, Kitashirakawa, Kyoto 606 8502, Japan
    Proc Natl Acad Sci U S A 98:2053-8. 2001
    ..The comparison of mutant phenotypes of gun5 and another Mg-chelatase subunit (ChlI) mutant suggests a specific function for ChlH protein in the plastid-signaling pathway...
  78. ncbi ABI5 acts downstream of ABI3 to execute an ABA-dependent growth arrest during germination
    Luis Lopez-Molina
    Laboratory of Plant Molecular Biology, Laboratory for Mass Spectrometry and Gaseous Ion Chemistry, The Rockefeller University, 1230 York Avenue, New York, NY 10021 6399, USA
    Plant J 32:317-28. 2002
    ..Although ABI5 is consistently located in the nucleus, chromosomal immunoprecipitation (ChIP) experiments revealed that ABA increases ABI5 occupancy on the AtEm6 promoter...
  79. ncbi Active genes dynamically colocalize to shared sites of ongoing transcription
    Cameron S Osborne
    Laboratory of Chromatin and Gene Expression, The Babraham Institute, Babraham Research Campus, Cambridge, CB2 4AT, UK
    Nat Genet 36:1065-71. 2004
    ..Thus, rather than recruiting and assembling transcription complexes, active genes migrate to preassembled transcription sites...
  80. ncbi Actin dynamics control SRF activity by regulation of its coactivator MAL
    Francesc Miralles
    Transcription Laboratory, Room 401, Cancer Research UK London Research Institute, Lincolns Inn Fields Laboratories, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
    Cell 113:329-42. 2003
    ..Constitutively cytoplasmic MAL derivatives interfere with MAL redistribution and Rho-actin signaling to SRF. MAL associates with several SRF target promoters regulated via the Rho-actin pathway...
  81. ncbi New insights into the role of the subnuclear structure ND10 for viral infection
    Nina Tavalai
    Institute for Clinical and Molecular Virology, University Erlangen Nuremberg, Schlossgarten 4, 91054 Erlangen, Germany
    Biochim Biophys Acta 1783:2207-21. 2008
    ....
  82. ncbi Mono- versus polyubiquitination: differential control of p53 fate by Mdm2
    Muyang Li
    Institute for Cancer Genetics and Department of Pathology, College of Physicians and Surgeons, Columbia University, 1150 St Nicholas Avenue, New York, NY 10032, USA
    Science 302:1972-5. 2003
    ..These results clarify the nature of ubiquitination-mediated p53 regulation and suggest that distinct mechanisms regulate p53 function in accordance with the levels of Mdm2 activity...
  83. ncbi Direct activation of Bax by p53 mediates mitochondrial membrane permeabilization and apoptosis
    Jerry E Chipuk
    Division of Cellular Immunology, La Jolla Institute for Allergy and Immunology, 10355 Science Center Drive, San Diego, CA 92121, USA
    Science 303:1010-4. 2004
    ..We propose that when p53 accumulates in the cytosol, it can function analogously to the BH3-only subset of proapoptotic Bcl-2 proteins to activate Bax and trigger apoptosis...
  84. ncbi Essential role for nuclear PTEN in maintaining chromosomal integrity
    Wen Hong Shen
    Department of Radiation Oncology, College of Physicians and Surgeons, Columbia University, 630 West 168th Street, New York, NY 10032, USA
    Cell 128:157-70. 2007
    ..We propose that PTEN acts as a guardian of genome integrity...
  85. ncbi Nuclear interaction of EGFR and STAT3 in the activation of the iNOS/NO pathway
    Hui Wen Lo
    Cancer Cell 7:575-89. 2005
    ..Our work suggests that the deregulated iNOS/NO pathway may partly contribute to the malignant biology of tumor cells with high levels of nuclear EGFR and STAT3...
  86. pmc A Begomovirus DNAbeta-encoded protein binds DNA, functions as a suppressor of RNA silencing, and targets the cell nucleus
    Xiaofeng Cui
    Institute of Biotechnology, Zhejiang University, Hangzhou 310029, People s Republic of China
    J Virol 79:10764-75. 2005
    ....
  87. ncbi Controlling the double helix
    Gary Felsenfeld
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Building 5, Room 212, Bethesda, Maryland 20892 0540, USA
    Nature 421:448-53. 2003
    ..Chromatin structure is dynamic and exerts profound control over gene expression and other fundamental cellular processes. Changes in its structure can be inherited by the next generation, independent of the DNA sequence itself...
  88. pmc Transportin-SR, a nuclear import receptor for SR proteins
    N Kataoka
    Howard Hughes Medical Institute, University of Pennsylvania School of Medicine, Department of Biochemistry and Biophysics, Philadelphia, Pennsylvania 19104 6148, USA
    J Cell Biol 145:1145-52. 1999
    ..TRN-SR has amino acid sequence similarity to several members of the importin beta/transportin family. These findings strongly suggest that TRN-SR is a nuclear import receptor for the SR protein family...
  89. ncbi Evidence for silencing compartments within the yeast nucleus: a role for telomere proximity and Sir protein concentration in silencer-mediated repression
    L Maillet
    , France
    Genes Dev 10:1796-811. 1996
    ..Efficient silencer function thus seems to require either proximity to a pool of concentrated Sir proteins, that is, proximity to telomeres, or delocalization of the silencing factors...
  90. pmc Deep sequencing of human nuclear and cytoplasmic small RNAs reveals an unexpectedly complex subcellular distribution of miRNAs and tRNA 3' trailers
    Jian You Liao
    State Key Laboratory of Biocontrol, Sun Yat Sen University, Guangzhou, People s Republic of China
    PLoS ONE 5:e10563. 2010
    ..However, recent studies have reported that some miRNAs localize to and function in the nucleus...
  91. doi Biogenesis of mRNPs: integrating different processes in the eukaryotic nucleus
    Rosa Luna
    Centro Andaluz de Biologia Molecular y Medicina Regenerativa CABIMER, Universidad de Sevilla CSIC, Seville, Spain
    Chromosoma 117:319-31. 2008
    ..Here, we review the overall process of mRNP biogenesis with particular emphasis on the functional coupling of transcription with mRNP biogenesis and export and its relationship to nuclear organization...
  92. pmc Transcription termination by nuclear RNA polymerases
    Patricia Richard
    Department of Biological Sciences, Columbia University, New York, New York 10027, USA
    Genes Dev 23:1247-69. 2009
    Gene transcription in the cell nucleus is a complex and highly regulated process. Transcription in eukaryotes requires three distinct RNA polymerases, each of which employs its own mechanisms for initiation, elongation, and termination...
  93. ncbi The specificity of the CRM1-Rev nuclear export signal interaction is mediated by RanGTP
    P Askjaer
    Department of Molecular and Structural Biology, University of Aarhus, C F Mollers Alle, Building 130, DK 8000 Aarhus C, Denmark
    J Biol Chem 273:33414-22. 1998
    ..Our data are consistent with a model in which CRM1 is the nuclear export receptor for the Rev-RRE ribonucleoprotein complex and that RanGTP binds to a preformed Rev-CRM1 complex and specifies a functional interaction with the NES...
  94. pmc Nucleus-vacuole junctions in Saccharomyces cerevisiae are formed through the direct interaction of Vac8p with Nvj1p
    X Pan
    Department of Biology, University of Rochester, Rochester NY, 14627, USA
    Mol Biol Cell 11:2445-57. 2000
    ..Overexpression of Nvj1p caused the profound proliferation of NV junctions. We conclude that Vac8p and Nvj1p are necessary components of a novel interorganelle junction apparatus...
  95. pmc JNK signaling in apoptosis
    D N Dhanasekaran
    Fels Institute for Cancer Research and Molecular Biology, Temple University School of Medicine, Philadelphia, PA 19140, USA
    Oncogene 27:6245-51. 2008
    ..This review analyses our present understanding of the role of JNK in apoptotic signaling and the various mechanisms by which JNK promotes apoptosis...
  96. pmc "Cotranscriptionality": the transcription elongation complex as a nexus for nuclear transactions
    Roberto Perales
    Department of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, UCHSC, MS8101, P O Box 6511, Aurora CO, 80045, USA
    Mol Cell 36:178-91. 2009
    ..From this perspective, TECs can be viewed as potent "community organizers" within the nucleus...
  97. pmc Nuclear factor 90 negatively regulates influenza virus replication by interacting with viral nucleoprotein
    Pui Wang
    Department of Microbiology, The University of Hong Kong, Pokfulam, People s Republic of China
    J Virol 83:7850-61. 2009
    ..These results suggest that NF90 inhibits influenza virus replication during the early phase of infection through direct interaction with viral NP...
  98. ncbi Mitochondrial DNA under siege in avian phylogeography
    Robert M Zink
    Bell Museum of Natural History, University of Minnesota, St Paul, MN 55108, USA
    Mol Ecol 17:2107-21. 2008
    ..Nuclear genes will prove important for quantitative estimates of the depths of haplotype trees, rates of population growth and values of gene flow...
  99. ncbi The fate of dsRNA in the nucleus: a p54(nrb)-containing complex mediates the nuclear retention of promiscuously A-to-I edited RNAs
    Z Zhang
    Department of Microbiology, University of Connecticut Health Center, Farmington, CT 06030, USA
    Cell 106:465-75. 2001
    ..We provide evidence that one function of the complex identified here is to anchor hyperedited RNAs to the nuclear matrix, while allowing selectively edited mRNAs to be exported...
  100. ncbi SIR3 and SIR4 proteins are required for the positioning and integrity of yeast telomeres
    F Palladino
    Swiss Institute for Experimental Cancer Research, Epalinges s Lausanne
    Cell 75:543-55. 1993
    ..These data suggest that SIR3 and SIR4 are required for both the integrity and subnuclear localization of yeast telomeres, the loss of which correlates with loss of telomere-associated gene repression...
  101. ncbi A calcineurin-dependent transcriptional pathway for cardiac hypertrophy
    J D Molkentin
    Department of Molecular Biology and Oncology, The University of Texas Southwestern Medical Center at Dallas, 75225 9148, USA
    Cell 93:215-28. 1998
    ..Pharmacologic inhibition of calcineurin activity blocks hypertrophy in vivo and in vitro. These results define a novel hypertrophic signaling pathway and suggest pharmacologic approaches to prevent cardiac hypertrophy and heart failure...

Research Grants81

  1. Nuclear bodies and ribonucleoprotein biogenesis
    MICHAEL HEBERT; Fiscal Year: 2009
    ..Certain diseases, such as cancer and some neurodegenerative disorders, may alter Cajal body and Gem activity. ..
  2. Cardiac Physiology and Imaging Core for Mouse in Vivo
    Kirk Peterson; Fiscal Year: 2009
    ..Chen) will access the personnel and resources of Core B in order to analyze the in vivo physiological and morphological characteristics of the mouse mutant intended to recapitulate Naxos disease, a recessive form of 262 ..
  3. Live cell imaging of IgH and c-Myc gene loci and the role of nuclear organization
    Mark T Groudine; Fiscal Year: 2010
    ..The cell nucleus is a highly ordered organelle...
  4. Live cell imaging of IgH and c-Myc gene loci and the role of nuclear organization
    Mark Groudine; Fiscal Year: 2009
    ..The cell nucleus is a highly ordered organelle...
  5. MEIOTIC CHROMOSOME SEGREGATION IN C ELEGANS
    Anne Villeneuve; Fiscal Year: 1999
    ..Selection for EMS induced suppressors of him-14 will be performed. These suppressor mutants will be analyzed for defects in meiosis. ..
  6. MEIOTIC CHROMOSOME SEGREGATION IN C ELEGANS
    Anne Villeneuve; Fiscal Year: 2000
    ..Selection for EMS induced suppressors of him-14 will be performed. These suppressor mutants will be analyzed for defects in meiosis. ..
  7. RECEPTOR-TARGETED COPPER RADIOPHARMACEUTICALS FOR CANCER IMAGING AND THERAPY
    Carolyn J Anderson; Fiscal Year: 2010
    ..radiotherapy, as well as questions regarding basic biochemistry of p53-mediated copper trafficking to the cell nucleus. PUBLIC HEALTH RELEVANCE: Copper-64 (T1/2 = 12.7 h;[unreadable]+ (17.8%);[unreadable]- (38...
  8. RECEPTOR-TARGETED COPPER RADIOPHARMACEUTICALS FOR CANCER IMAGING AND THERAPY
    Carolyn Anderson; Fiscal Year: 2009
    ..radiotherapy, as well as questions regarding basic biochemistry of p53-mediated copper trafficking to the cell nucleus. PUBLIC HEALTH RELEVANCE: Copper-64 (T1/2 = 12.7 h;[unreadable]+ (17.8%);[unreadable]- (38...
  9. Role of Lamins in Endothelial Mechanotransduction at the Nucleus
    Julie Y Ji; Fiscal Year: 2010
    ..GR as a model, the proposed project will further examine the dynamics interactions of shear activated GR at the cell nucleus. Underlying the nuclear envelope is the lamina, a network of type V intermediate filaments, lamins, which ..
  10. Molecular Mechanism of Hepadnavirus Persistence
    Jianming Hu; Fiscal Year: 2010
    ..the episomal viral DNA, the so-called covalently closed circular (CCC) DNA, persists in the infected cell nucleus even after years of antiviral treatment...
  11. Molecular Mechanism of Hepadnavirus Persistence
    Jianming Hu; Fiscal Year: 2009
    ..the episomal viral DNA, the so-called covalently closed circular (CCC) DNA, persists in the infected cell nucleus even after years of antiviral treatment...
  12. Neuropeptide regulation of neurohypophyseal function
    FRANCIS FLYNN; Fiscal Year: 2009
    ..NK3R expressed by VP magnocellular neurons were shown to be translocated from the cell membrane to the cell nucleus by confocal microscopy, immuno-electron microscopy and Western blot...
  13. Neuropeptide regulation of neurohypophyseal function
    Francis W Flynn; Fiscal Year: 2010
    ..NK3R expressed by VP magnocellular neurons were shown to be translocated from the cell membrane to the cell nucleus by confocal microscopy, immuno-electron microscopy and Western blot...
  14. ATP LINKED EFFECTORS OF NA+, K+ ATPASE AND CATARACTS
    MARGARET GARNER; Fiscal Year: 2000
    ..Na,K-ATPases are also present in cell nucleus and endoplasmic reticulum of cultured lens epithelial cells and in the cell nucleus and ER of rat liver...
  15. ATP LINKED EFFECTORS OF NA+, K+ ATPASE AND CATARACTS
    MARGARET GARNER; Fiscal Year: 1999
    ..Na,K-ATPases are also present in cell nucleus and endoplasmic reticulum of cultured lens epithelial cells and in the cell nucleus and ER of rat liver...
  16. ATP LINKED EFFECTORS OF NA+, K+ ATPASE AND CATARACTS
    MARGARET GARNER; Fiscal Year: 2001
    ..Na,K-ATPases are also present in cell nucleus and endoplasmic reticulum of cultured lens epithelial cells and in the cell nucleus and ER of rat liver...
  17. Defective nuclear FGFR1 signaling - a new model for PD
    MICHAL STACHOWIAK; Fiscal Year: 2003
    ..In the Snc the most abundant are the HMW non-released isoforms of FGF-2 that localize almost exclusively in the cell nucleus. Functions of nuclear FGF-2 and the significance of its depletion in Snc neurons in PD patients remain unknown...
  18. ESTROGEN RECEPTOR-DIRECTED THERAPY WITH AUGER ELECTRONS
    EUGENE DE SOMBRE; Fiscal Year: 1990
    ..emitting, receptor- binding ligands would be expected to depend only upon the presence of the receptor in the cell nucleus and, by causing double stranded DNA breads, should kill the cells...
  19. Defective nuclear FGFR1 signaling - a new model for PD
    MICHAL STACHOWIAK; Fiscal Year: 2002
    ..In the Snc the most abundant are the HMW non-released isoforms of FGF-2 that localize almost exclusively in the cell nucleus. Functions of nuclear FGF-2 and the significance of its depletion in Snc neurons in PD patients remain unknown...
  20. NEUROENDOCRINE REGULATION OF BEHAVIORS
    JEFFREY BLAUSTEIN; Fiscal Year: 1992
    ..Finally, the hypothesis that vagino-cervical stimulation activates certain neurons via noradrenergic receptors will be tested...
  21. NEUROENDOCRINE REGULATION OF BEHAVIORS
    JEFFREY BLAUSTEIN; Fiscal Year: 1993
    ..Finally, the hypothesis that vagino-cervical stimulation activates certain neurons via noradrenergic receptors will be tested...
  22. NOVEL APPROACHES TO RADIO-IMMUNOCONJUGATE DOSIMETRY
    Lee Chin; Fiscal Year: 1990
    ..The dosimetric tools developed here will be appropriate for later application to clinical radio-immunotherapy...
  23. Intravenous Protein Therapy for Myotonic Dystrophy Type 1
    DUSTIN ARMSTRONG; Fiscal Year: 2009
    ..3E10 is a murine-derived monoclonal antibody that penetrates living cells and localizes to the cell nucleus without apparent injury to target cells (22, 23)...
  24. The Mammalian Cellular Splicing Machine - Structure and Function
    Ruth Sperling; Fiscal Year: 2009
    ..The long term objective of this project is to understand the regulation of splicing and alternative splicing, thus recognizing how defects in these important processes affect human diseases. ..
  25. Accelerating Cancer Research with Single Cell Arrays
    Heinz Ulrich Weier; Fiscal Year: 2009
    ..Due to their versatility, SCAs may become powerful tools in basic and clinical research, thereby benefiting patients with de novo translocations or premalignant lesions as well as cancer patients. ..
  26. The Mammalian Cellular Splicing Machine - Structure and Function
    Ruth Sperling; Fiscal Year: 2010
    ..The long term objective of this project is to understand the regulation of splicing and alternative splicing, thus recognizing how defects in these important processes affect human diseases. ..
  27. Mechanism of Apoptosis and Inhibition of Tumor Progression and Metastasis by Par-
    Vivek M Rangnekar; Fiscal Year: 2010
    ..Although ectopic Par-4 and the SAC domain have long been thought to effect apoptosis by acting within the cell nucleus to inhibit NF-kappaB, our recent transient transfection studies unexpectedly displayed apoptosis in neighboring ..
  28. Mechanism of Apoptosis and Inhibition of Tumor Progression and Metastasis by Par-
    Vivek Rangnekar; Fiscal Year: 2009
    ..Although ectopic Par-4 and the SAC domain have long been thought to effect apoptosis by acting within the cell nucleus to inhibit NF-kappaB, our recent transient transfection studies unexpectedly displayed apoptosis in neighboring ..
  29. Mechanism of Apoptosis and Inhibition of Tumor Progression and Metastasis by Par-
    Vivek Rangnekar; Fiscal Year: 2009
    ..Although ectopic Par-4 and the SAC domain have long been thought to effect apoptosis by acting within the cell nucleus to inhibit NF-kappaB, our recent transient transfection studies unexpectedly displayed apoptosis in neighboring ..
  30. SURFACE ANTIGENS OF ORAL BACTEROIDS SPECIES
    Ann Progulske Fox; Fiscal Year: 1992
    ..The information is imparted to the epithelial cell nucleus and genome ultimately inducing the expression of a finite family of genes...
  31. PHOSPHORYLATION AND TRANSPORT OF VIRAL NUCLEOPROTEINS
    Mario Stevenson; Fiscal Year: 2001
    ..property of HIV-1 is governed by the ability of the reverse transcription complex to translocate to the host cell nucleus in the absence of mitosis...
  32. PHOSPHORYLATION AND TRANSPORT OF VIRAL NUCLEOPROTEINS
    Mario Stevenson; Fiscal Year: 2000
    ..property of HIV-1 is governed by the ability of the reverse transcription complex to translocate to the host cell nucleus in the absence of mitosis...
  33. ASSEMBLY OF THE HERPES SIMPLEX VIRUS CAPSID
    Jay C Brown; Fiscal Year: 2010
    ..The capsid, which contains the virus DNA, is assembled in the infected cell nucleus. A DNA-free capsid shell is first formed and later packaged with DNA...
  34. ASSEMBLY OF THE HERPES SIMPLEX VIRUS CAPSID
    Jay Brown; Fiscal Year: 2009
    ..The capsid, which contains the virus DNA, is assembled in the infected cell nucleus. A DNA-free capsid shell is first formed and later packaged with DNA...
  35. PHOSPHORYLATION AND TRANSPORT OF VIRAL NUCLEOPROTEINS
    Mario Stevenson; Fiscal Year: 1999
    ..property of HIV-1 is governed by the ability of the reverse transcription complex to translocate to the host cell nucleus in the absence of mitosis...
  36. Histone Tail Interactions and Functions in Chromatin
    Jeffrey J Hayes; Fiscal Year: 2010
    DNA within the eukaryotic cell nucleus is assembled with histones and other proteins to form a complicated, multifaceted complex known as chromatin...
  37. ASSEMBLY OF THE HERPES SIMPLEX VIRUS CAPSID
    Jay Brown; Fiscal Year: 2007
    ..The capsid, which contains the virus DMA, is assembled in the infected cell nucleus. A DNA-free capsid shell is first formed and later packaged with DNA...
  38. Misregulation of apoptosis in cloned pig embryos
    Heide Schatten; Fiscal Year: 2006
    ..1% in pigs) for which causes are only little understood. Incomplete or abnormal remodeling of the donor cell nucleus following transfer, resulting in imprinting failures and abnormal gene expression throughout development, are ..
  39. Misregulation of apoptosis in cloned pig embryos
    Heide Schatten; Fiscal Year: 2007
    ..1% in pigs) for which causes are only little understood. Incomplete or abnormal remodeling of the donor cell nucleus following transfer, resulting in imprinting failures and abnormal gene expression throughout development, are ..
  40. Long-Circulating Polymer-Modified Liposomes
    Vladimir Torchilin; Fiscal Year: 2007
    ..abstract_text> ..
  41. THE ORGANIZATION OF ANIMAL CELL NUCLEI
    Joseph G Gall; Fiscal Year: 2010
    The long-term aim of our research is to understand the functional organization of the animal cell nucleus. Specifically, we will focus on the non-chromosomal components of the nucleus, collectively referred to as nuclear bodies...
  42. LABELING OF OCTREOTIDE WITH POSITRON EMITTERS
    Carolyn Anderson; Fiscal Year: 2003
    ..Aim 2 will also involve the estimation of absorbed doses to the cell nucleus from Cu-64-TETA-Y3-TATE and Cu-64-CB-TE2A-Y3-TATE...
  43. LABELING OF OCTREOTIDE WITH POSITRON EMITTERS
    Carolyn Anderson; Fiscal Year: 2005
    ..Aim 2 will also involve the estimation of absorbed doses to the cell nucleus from Cu-64-TETA-Y3-TATE and Cu-64-CB-TE2A-Y3-TATE...
  44. LABELING OF OCTREOTIDE WITH POSITRON EMITTERS
    Carolyn Anderson; Fiscal Year: 2004
    ..Aim 2 will also involve the estimation of absorbed doses to the cell nucleus from Cu-64-TETA-Y3-TATE and Cu-64-CB-TE2A-Y3-TATE...
  45. THE ORGANIZATION OF ANIMAL CELL NUCLEI
    Joseph Gall; Fiscal Year: 2009
    The long-term aim of our research is to understand the functional organization of the animal cell nucleus. Specifically, we will focus on the non-chromosomal components of the nucleus, collectively referred to as nuclear bodies...
  46. LABELING OF OCTREOTIDE WITH POSITRON EMITTERS
    Carolyn Anderson; Fiscal Year: 2006
    ..Aim 2 will also involve the estimation of absorbed doses to the cell nucleus from Cu-64-TETA-Y3-TATE and Cu-64-CB-TE2A-Y3-TATE...
  47. Proteomic Characterization of IC Bladder
    Fernando DeMiguel; Fiscal Year: 2003
    ..The nuclear matrix is the structural scaffolding of the cell nucleus and plays a central role in the regulation of important cellular processes...
  48. GENETICS OF HERPESVIRUS TRANSFORMATION
    David Knipe; Fiscal Year: 2009
    ..goals of this research are to define 1) the mechanisms by which herpes simplex virus (HSV) remodels the host cell nucleus to optimize its replication and 2) the functions and mechanisms of action of the HSV infected cell protein 8 (..
  49. Proteomic Characterization of IC Bladder
    Fernando DeMiguel; Fiscal Year: 2005
    ..The nuclear matrix is the structural scaffolding of the cell nucleus and plays a central role in the regulation of important cellular processes...
  50. Proteomic Characterization of IC Bladder
    Fernando DeMiguel; Fiscal Year: 2004
    ..The nuclear matrix is the structural scaffolding of the cell nucleus and plays a central role in the regulation of important cellular processes...
  51. Proteomic Characterization of IC Bladder
    Pradeep Tyagi; Fiscal Year: 2006
    ..The nuclear matrix is the structural scaffolding of the cell nucleus and plays a central role in the regulation of important cellular processes...
  52. Accelerating Cancer Research with Single Cell Arrays
    Heinz Ulrich Weier; Fiscal Year: 2009
    ..This will open new avenues for the analysis of small samples like those obtained by fine needle biopsies as well as the analysis of circulating or exfoliated tumor cells. ..
  53. REGULATION OF NUCLEAR ENVELOPE ASSEMBLY AND DISASSEMBLY
    Katherine Wilson; Fiscal Year: 2009
    ..The proposed studies of emerin and BAF in cellular responses to DNA damage are relevant to cancer and human aging. ..
  54. REGULATION OF NUCLEAR ENVELOPE ASSEMBLY AND DISASSEMBLY
    Katherine L Wilson; Fiscal Year: 2010
    ..The proposed studies of emerin and BAF in cellular responses to DNA damage are relevant to cancer and human aging. ..
  55. REGULATION OF GERMLINE STEM CELL DIVISION IN DROSOPHILA
    Haifan Lin; Fiscal Year: 1999
    ..The fourth specific aim is to analyze the molecular structure of these two genes and to determine the nature of their protein products. The final specific aim is to analyze the cellular behavior of the two proteins. ..
  56. MOLECULAR CHARACTERIZATION OF HERPES SIMPLEX VIRUS ICP27
    Stephen Rice; Fiscal Year: 2003
    ..of mammalian cells with herpes simplex virus type 1 (HSV-1) results in dramatic alterations to the host cell nucleus, so that viral genes are expressed at high levels while cellular genes are nearly completely suppressed...
  57. Histone Tail Interactions and Functions in Chromatin
    JEFFREY HAYES; Fiscal Year: 2009
    DNA within the eukaryotic cell nucleus is assembled with histones and other proteins to form a complicated, multifaceted complex known as chromatin...
  58. Histone Tail Interactions and Functions in Chromatin
    Jeffrey J Hayes; Fiscal Year: 2011
    DNA within the eukaryotic cell nucleus is assembled with histones and other proteins to form a complicated, multifaceted complex known as chromatin...
  59. PROTEIN BINDING INTERACTIONS OF THYROACTIVE COMPOUNDS
    Vivian Cody; Fiscal Year: 1993
    ..are bound to proteins when transported through the general circulation, when at the cell membrane or in the cell nucleus, identification of the molecular features responsible for binding is of great importance...
  60. Histone Tail Interactions and Functions in Chromatin
    JEFFREY HAYES; Fiscal Year: 2009
    DNA within the eukaryotic cell nucleus is assembled with histones and other proteins to form a complicated, multifaceted complex known as chromatin...
  61. Signal Transduction in Myelinating Cells
    Charles Stiles; Fiscal Year: 2003
    ..the loop/incisure regions, 2) determine whether activated erbB2 has remote signaling functions in the Schwann cell nucleus and 3) determine the cellular fate of erbB2 following activation at the loop/incisure domains...
  62. NUCLEAR STRUCTURE AND METAZOAN ORIGINS OF REPLICATION
    David Gilbert; Fiscal Year: 1999
    ..We will then determine which steps in the assembly of functional replication origins are interrupted by these disruptions in nuclear structure. ..
  63. GENETICS OF HERPESVIRUS TRANSFORMATION
    David Knipe; Fiscal Year: 2007
    ..transcription and the mechanisms of assembly and maturation of viral replication compartments in the infected cell nucleus. In this application our specific aims are to: 1...
  64. GENETICS OF HERPESVIRUS TRANSFORMATION
    David Knipe; Fiscal Year: 2006
    ..transcription and the mechanisms of assembly and maturation of viral replication compartments in the infected cell nucleus. In this application our specific aims are to: 1...
  65. GENETICS OF HERPESVIRUS TRANSFORMATION
    David Knipe; Fiscal Year: 2005
    ..transcription and the mechanisms of assembly and maturation of viral replication compartments in the infected cell nucleus. In this application our specific aims are to: 1...
  66. NUCLEAR STRUCTURE AND METAZOAN ORIGINS OF REPLICATION
    David Gilbert; Fiscal Year: 2000
    ..We will then determine which steps in the assembly of functional replication origins are interrupted by these disruptions in nuclear structure. ..
  67. GENETICS OF HERPESVIRUS TRANSFORMATION
    David Knipe; Fiscal Year: 2004
    ..transcription and the mechanisms of assembly and maturation of viral replication compartments in the infected cell nucleus. In this application our specific aims are to: 1...
  68. NUCLEAR STRUCTURE AND METAZOAN ORIGINS OF REPLICATION
    David Gilbert; Fiscal Year: 2001
    ..We will then determine which steps in the assembly of functional replication origins are interrupted by these disruptions in nuclear structure. ..
  69. Biomaterials that Promote Healing & Prevent Infection
    JAMES BRYERS; Fiscal Year: 2002
    ..processes are initiated and controlled by a communication pathway from stimulus, to cell surface receptor, to cell nucleus, to mRNA, to cytokine signaling agents and higher tissue response...
  70. Biomaterials that Promote Healing & Prevent Infection
    JAMES BRYERS; Fiscal Year: 2004
    ..processes are initiated and controlled by a communication pathway from stimulus, to cell surface receptor, to cell nucleus, to mRNA, to cytokine signaling agents and higher tissue response...
  71. Biomaterials that Promote Healing & Prevent Infection
    JAMES BRYERS; Fiscal Year: 2003
    ..processes are initiated and controlled by a communication pathway from stimulus, to cell surface receptor, to cell nucleus, to mRNA, to cytokine signaling agents and higher tissue response...
  72. Biomaterials that Promote Healing & Prevent Infection
    JAMES BRYERS; Fiscal Year: 2003
    ..processes are initiated and controlled by a communication pathway from stimulus, to cell surface receptor, to cell nucleus, to mRNA, to cytokine signaling agents and higher tissue response...
  73. Core Histone Tall Interactions and Function in Chromatin
    JEFFREY HAYES; Fiscal Year: 2007
    Within the eukaryotic cell nucleus, DNA is associated with core histones to form nucleosomes; which are further assembled with ancillary proteins into a multi-faceted complex known as chromatin...
  74. Core Histone Tall Interactions and Function in Chromatin
    JEFFREY HAYES; Fiscal Year: 2004
    Within the eukaryotic cell nucleus, DNA is associated with core histones to form nucleosomes; which are further assembled with ancillary proteins into a multi-faceted complex known as chromatin...
  75. THYROXINE BINDING PROTEIN AND HORMONE UTILIZATION
    KENNETH STERLING; Fiscal Year: 1980
    ..has not previously been reported, leading to the supposition that saturable receptors exist only in the cell nucleus. The present work shows for the first time that in vivo displacement of tracer (125I)-T3 is regularly observed ..
  76. Methods Development for CVM Cell Migration Study in Drosophila
    ANGELIKE M STATHOPOULOS; Fiscal Year: 2012
    ..As development of the gastrointestinal tract of animals is highly similar, studies of how CVM cell migration influences gut formation in Drosophila have the potential for far- reaching implications. ..
  77. EFFECT OF CLONING IN EPIGENETIC INHERITANCE IN MICE
    Keith Latham; Fiscal Year: 2000
    ..some forms of epigenetic inheritance, such as genomic imprinting, must be maintained in order for a somatic cell nucleus to support normal development...
  78. EFFECT OF CLONING IN EPIGENETIC INHERITANCE IN MICE
    Keith Latham; Fiscal Year: 2001
    ..some forms of epigenetic inheritance, such as genomic imprinting, must be maintained in order for a somatic cell nucleus to support normal development...