cell nucleus


Summary: Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)

Top Publications

  1. pmc Comprehensive mapping of long-range interactions reveals folding principles of the human genome
    Erez Lieberman-Aiden
    Broad Institute of Harvard and Massachusetts Institute of Technology MIT, MA 02139, USA
    Science 326:289-93. 2009
  2. ncbi Capturing chromosome conformation
    Job Dekker
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    Science 295:1306-11. 2002
  3. ncbi The Wnt signaling pathway in development and disease
    Catriona Y Logan
    Department of Developmental Biology, Beckman Center, Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA
    Annu Rev Cell Dev Biol 20:781-810. 2004
  4. pmc Exportin-5 mediates the nuclear export of pre-microRNAs and short hairpin RNAs
    Rui Yi
    Howard Hughes Medical Institute, Duke University Medical Center, Durham, NC 27710, USA
    Genes Dev 17:3011-6. 2003
  5. pmc The nuclear-retained noncoding RNA MALAT1 regulates alternative splicing by modulating SR splicing factor phosphorylation
    Vidisha Tripathi
    Department of Cell and Developmental Biology, University of Illinois at Urbana Champaign, Urbana, IL 61801, USA
    Mol Cell 39:925-38. 2010
  6. ncbi Sensing DNA damage through ATRIP recognition of RPA-ssDNA complexes
    Lee Zou
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Science 300:1542-8. 2003
  7. ncbi Nuclear export of microRNA precursors
    Elsebet Lund
    Department of Biomolecular Chemistry, University of Wisconsin Medical School, Madison, WI 53706, USA
    Science 303:95-8. 2004
  8. pmc A three-dimensional model of the yeast genome
    Zhijun Duan
    Institute for Stem Cell and Regenerative Medicine, University of Washington, Seattle, Washington 98195 8056, USA
    Nature 465:363-7. 2010
  9. pmc Elucidation of a universal size-control mechanism in Drosophila and mammals
    Jixin Dong
    Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Cell 130:1120-33. 2007
  10. ncbi Functional interaction of beta-catenin with the transcription factor LEF-1
    J Behrens
    Max Delbruck Centre for Molecular Medicine, Berlin, Germany
    Nature 382:638-42. 1996

Detail Information

Publications368 found, 100 shown here

  1. pmc Comprehensive mapping of long-range interactions reveals folding principles of the human genome
    Erez Lieberman-Aiden
    Broad Institute of Harvard and Massachusetts Institute of Technology MIT, MA 02139, USA
    Science 326:289-93. 2009
    ..The fractal globule is distinct from the more commonly used globular equilibrium model. Our results demonstrate the power of Hi-C to map the dynamic conformations of whole genomes...
  2. ncbi Capturing chromosome conformation
    Job Dekker
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    Science 295:1306-11. 2002
    ..Furthermore, functionally distinct AT- and GC-rich domains were found to exhibit different conformations, and a population-average 3D model of chromosome III could be determined. Chromosome III emerges as a contorted ring...
  3. ncbi The Wnt signaling pathway in development and disease
    Catriona Y Logan
    Department of Developmental Biology, Beckman Center, Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA
    Annu Rev Cell Dev Biol 20:781-810. 2004
    ..The next few years are likely to see novel therapeutic reagents aimed at controlling Wnt signaling in order to alleviate these conditions...
  4. pmc Exportin-5 mediates the nuclear export of pre-microRNAs and short hairpin RNAs
    Rui Yi
    Howard Hughes Medical Institute, Duke University Medical Center, Durham, NC 27710, USA
    Genes Dev 17:3011-6. 2003
    ..Together, these findings define an additional cellular cofactor required for miRNA biogenesis and function...
  5. pmc The nuclear-retained noncoding RNA MALAT1 regulates alternative splicing by modulating SR splicing factor phosphorylation
    Vidisha Tripathi
    Department of Cell and Developmental Biology, University of Illinois at Urbana Champaign, Urbana, IL 61801, USA
    Mol Cell 39:925-38. 2010
    ..Taken together, our results suggest that MALAT1 regulates AS by modulating the levels of active SR proteins. Our results further highlight the role for an nrRNA in the regulation of gene expression...
  6. ncbi Sensing DNA damage through ATRIP recognition of RPA-ssDNA complexes
    Lee Zou
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Science 300:1542-8. 2003
    ..Our data suggest that RPA-coated ssDNA is the critical structure at sites of DNA damage that recruits the ATR-ATRIP complex and facilitates its recognition of substrates for phosphorylation and the initiation of checkpoint signaling...
  7. ncbi Nuclear export of microRNA precursors
    Elsebet Lund
    Department of Biomolecular Chemistry, University of Wisconsin Medical School, Madison, WI 53706, USA
    Science 303:95-8. 2004
    ..Thus, Exp5 is key to miRNA biogenesis and may help coordinate nuclear and cytoplasmic processing steps...
  8. pmc A three-dimensional model of the yeast genome
    Zhijun Duan
    Institute for Stem Cell and Regenerative Medicine, University of Washington, Seattle, Washington 98195 8056, USA
    Nature 465:363-7. 2010
    ..Finally, we constructed a three-dimensional model of the yeast genome. Our findings provide a glimpse of the interface between the form and function of a eukaryotic genome...
  9. pmc Elucidation of a universal size-control mechanism in Drosophila and mammals
    Jixin Dong
    Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Cell 130:1120-33. 2007
    ..These results uncover a universal size-control mechanism in metazoan...
  10. ncbi Functional interaction of beta-catenin with the transcription factor LEF-1
    J Behrens
    Max Delbruck Centre for Molecular Medicine, Berlin, Germany
    Nature 382:638-42. 1996
    ..Thus beta-catenin regulates gene expression by direct interaction with transcription factors such as LEF-1, providing a molecular mechanism for the transmission of signals, from cell-adhesion components or wnt protein to the nucleus...
  11. ncbi Nuclear organization of active and inactive chromatin domains uncovered by chromosome conformation capture-on-chip (4C)
    Marieke Simonis
    Department of Cell Biology and Genetics, Erasmus Medical Centre, PO Box 2040, 3000 CA Rotterdam, The Netherlands
    Nat Genet 38:1348-54. 2006
    The spatial organization of DNA in the cell nucleus is an emerging key contributor to genomic function...
  12. ncbi A critical role for histone H2AX in recruitment of repair factors to nuclear foci after DNA damage
    T T Paull
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892 0540, USA
    Curr Biol 10:886-95. 2000
    ..Recently it has been reported that a member of the histone H2A family, H2AX, becomes extensively phosphorylated within 1-3 minutes of DNA damage and forms foci at break sites...
  13. ncbi Notch signaling: cell fate control and signal integration in development
    S Artavanis-Tsakonas
    Massachusetts General Hospital Cancer Center, Department of Cell Biology, Harvard Medical School, Building 149, 13th Street, Charlestown, MA 02129, USA
    Science 284:770-6. 1999
    ..Notch activity affects the implementation of differentiation, proliferation, and apoptotic programs, providing a general developmental tool to influence organ formation and morphogenesis...
  14. pmc The obesity-associated FTO gene encodes a 2-oxoglutarate-dependent nucleic acid demethylase
    Thomas Gerken
    Chemistry Research Laboratory and Oxford Centre for Integrative Systems Biology, University of Oxford, 12 Mansfield Road, Oxford, Oxon OX1 3TA, UK
    Science 318:1469-72. 2007
    ..Studies can now be directed toward determining the physiologically relevant FTO substrate and how nucleic acid methylation status is linked to increased fat mass...
  15. ncbi High-mobility group box 1 protein (HMGB1): nuclear weapon in the immune arsenal
    Michael T Lotze
    University of Pittsburgh School of Medicine, Room 411, 300 Technology Drive, Pittsburgh, Pennsylvania 15219, USA
    Nat Rev Immunol 5:331-42. 2005
    ..Here, we discuss these features of HMGB1 and summarize recent advances that have led to the preclinical development of therapeutics that modulate HMGB1 release and activity...
  16. ncbi Protein modification by SUMO
    Erica S Johnson
    Department of Biochemistry and Molecular Pharmacology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    Annu Rev Biochem 73:355-82. 2004
    ..This review discusses our current understanding of how SUMO conjugation is controlled, as well as the roles of SUMO in a number of biological processes...
  17. pmc Subdiffraction multicolor imaging of the nuclear periphery with 3D structured illumination microscopy
    Lothar Schermelleh
    Center for Integrated Protein Science, Department of Biology, Ludwig Maximilians University Munich, 82152 Planegg Martinsried, Germany
    Science 320:1332-6. 2008
    ..Multicolor 3D-SIM opens new and facile possibilities to analyze subcellular structures beyond the diffraction limit of the emitted light...
  18. ncbi Aggregation of huntingtin in neuronal intranuclear inclusions and dystrophic neurites in brain
    M Difiglia
    Department of Neurology, Massachusetts General Hospital, Boston, MA 02114, USA
    Science 277:1990-3. 1997
    ..Ubiquitin was also found in NIIs and DNs, which suggests that abnormal huntingtin is targeted for proteolysis but is resistant to removal. The aggregation of mutant huntingtin may be part of the pathogenic mechanism in HD...
  19. ncbi Oscillations in NF-kappaB signaling control the dynamics of gene expression
    D E Nelson
    Centre for Cell Imaging, School of Biological Sciences, Bioscience Research Building, Crown Street, Liverpool, L69 7ZB, UK
    Science 306:704-8. 2004
    ..The functional consequences of NF-kappaB signaling may thus depend on number, period, and amplitude of oscillations...
  20. pmc An architectural role for a nuclear noncoding RNA: NEAT1 RNA is essential for the structure of paraspeckles
    Christine M Clemson
    University of Massachusetts Medical Center, 55 Lake Avenue North, Worcester, MA 01655, USA
    Mol Cell 33:717-26. 2009
    ..Collectively, results demonstrate that NEAT1 functions as an essential structural determinant of paraspeckles, providing a precedent for a ncRNA as the foundation of a nuclear domain...
  21. ncbi Endosymbiotic gene transfer: organelle genomes forge eukaryotic chromosomes
    Jeremy N Timmis
    School of Molecular and Biomedical Science, The University of Adelaide, South Australia 5005, Australia
    Nat Rev Genet 5:123-35. 2004
  22. pmc Systematic identification of cell cycle-dependent yeast nucleocytoplasmic shuttling proteins by prediction of composite motifs
    Shunichi Kosugi
    Institute for Advanced Biosciences, Keio University, Tsuruoka, Japan
    Proc Natl Acad Sci U S A 106:10171-6. 2009
    ..The application of this strategy to other functional linear motifs should be useful in systematic studies of protein-protein networks...
  23. pmc Recruitment of human muscleblind proteins to (CUG)(n) expansions associated with myotonic dystrophy
    J W Miller
    Department of Molecular Genetics and Microbiology, Centers for Gene Therapy and Mammalian Genetics, University of Florida College of Medicine, Gainesville, FL 32610, USA
    EMBO J 19:4439-48. 2000
    ..We propose that DM1 disease is caused by aberrant recruitment of the EXP proteins to the DMPK transcript (CUG)(n) expansion...
  24. ncbi Mechanisms of TGF-beta signaling from cell membrane to the nucleus
    Yigong Shi
    Department of Molecular Biology, Princeton University, Washington Road, Princeton, NJ 08544, USA
    Cell 113:685-700. 2003
    ..This article reviews these latest advances and presents our current understanding on the mechanisms of TGF-beta signaling from cell membrane to the nucleus...
  25. ncbi Transcriptional paradigms in mammalian mitochondrial biogenesis and function
    Richard C Scarpulla
    Department of Cell and Molecular Biology, Northwestern Medical School, Chicago, Illinois 60611, USA
    Physiol Rev 88:611-38. 2008
    ..These transcriptional paradigms provide a basic framework for understanding the integration of mitochondrial biogenesis and function with signaling events that dictate cell- and tissue-specific energetic properties...
  26. ncbi Molecular characterization of mitochondrial apoptosis-inducing factor
    S A Susin
    Centre National de la Recherche Scientifique, UPR 420, Villejuif, France
    Nature 397:441-6. 1999
    ..These results indicate that AIF is a mitochondrial effector of apoptotic cell death...
  27. ncbi The biogeography of mitochondrial and nuclear discordance in animals
    David P L Toews
    Department of Zoology and Biodiversity Research Centre, University of British Columbia, 6270 University Blvd, Vancouver, BC V6T 1Z4, Canada
    Mol Ecol 21:3907-30. 2012
    ..Indeed, there is great potential for certain cases of mitochondrial introgression to become important natural systems within which to test the effect of different mitochondrial genotypes on whole-animal phenotypes...
  28. pmc Coupling of the nucleus and cytoplasm: role of the LINC complex
    Melissa Crisp
    Department of Anatomy and Cell Biology, University of Florida, Gainesville, FL 32610
    J Cell Biol 172:41-53. 2006
    ..2005), support a model in which Sun proteins tether nesprins in the ONM via interactions spanning the PNS. In this way, Sun proteins and nesprins form a complex that links the nucleoskeleton and cytoskeleton (the LINC complex)...
  29. ncbi DMC1: a meiosis-specific yeast homolog of E. coli recA required for recombination, synaptonemal complex formation, and cell cycle progression
    D K Bishop
    Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, Massachusetts 02138
    Cell 69:439-56. 1992
    ..DMC1 is homologous to yeast's RAD51 gene, supporting the view that mitotic DSB repair has been recruited for use in meiotic chromosome metabolism...
  30. ncbi The nuclear RNase III Drosha initiates microRNA processing
    Yoontae Lee
    Institute of Molecular Biology and Genetics and School of Biological Sciences, Seoul National University, Seoul 151 742, Korea
    Nature 425:415-9. 2003
    ..Thus, the two RNase III proteins, Drosha and Dicer, may collaborate in the stepwise processing of miRNAs, and have key roles in miRNA-mediated gene regulation in processes such as development and differentiation...
  31. ncbi Cryptic pol II transcripts are degraded by a nuclear quality control pathway involving a new poly(A) polymerase
    Françoise Wyers
    Equipe labelisée La Ligue, Avenue de la Terrasse, 91190 Gif sur Yvette, Paris, France
    Cell 121:725-37. 2005
    ..Our data strongly support the existence of a posttranscriptional quality control mechanism limiting inappropriate expression of genetic information...
  32. pmc Interactions between nuclei and the cytoskeleton are mediated by SUN-KASH nuclear-envelope bridges
    Daniel A Starr
    Department of Molecular and Cellular Biology, University of California, Davis, California 95616, USA
    Annu Rev Cell Dev Biol 26:421-44. 2010
    ..Knockouts of SUN or KASH proteins disrupt neurological and muscular development in mice. Defects in SUN and KASH proteins have been linked to human diseases including muscular dystrophy, ataxia, progeria, lissencephaly, and cancer...
  33. pmc Flexible use of nuclear import pathways by HIV-1
    Kyeongeun Lee
    HIV Drug Resistance Program, National Cancer Institute, Frederick, MD 21702, USA
    Cell Host Microbe 7:221-33. 2010
    ..These findings reveal a remarkable flexibility in HIV-1 nuclear transport and highlight a single residue in CA as essential in regulating interactions with NUPs...
  34. ncbi RNA degradation by the exosome is promoted by a nuclear polyadenylation complex
    John LaCava
    Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh EH9 3JR, UK
    Cell 121:713-24. 2005
    ..We speculate that this function was maintained in eukaryotic nuclei, while cytoplasmic mRNA poly(A) tails acquired different roles in translation...
  35. pmc BASC, a super complex of BRCA1-associated proteins involved in the recognition and repair of aberrant DNA structures
    Y Wang
    Verna and Mars McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas 77030, USA
    Genes Dev 14:927-39. 2000
    ..Collectively, these results suggest that BRCA1 may function as a coordinator of multiple activities required for maintenance of genomic integrity during the process of DNA replication and point to a central role for BRCA1 in DNA repair...
  36. ncbi Cellular motility driven by assembly and disassembly of actin filaments
    Thomas D Pollard
    Department of Cellular, Molecular, and Developmental Biology, Yale University, New Haven, CT 06520, USA
    Cell 112:453-65. 2003
    ..Profilin catalyzes the exchange of ADP for ATP, refilling the pool of ATP-actin monomers bound to profilin, ready for elongation...
  37. ncbi Exporting RNA from the nucleus to the cytoplasm
    Alwin Köhler
    Biochemie Zentrum der Universitat Heidelberg, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany
    Nat Rev Mol Cell Biol 8:761-73. 2007
    ..Export of mRNAs is unique as it is extensively coupled to transcription (in yeast) and splicing (in metazoa). Understanding the mechanisms that connect RNP formation with export is a major challenge in the field...
  38. ncbi Coordination of gene expression between organellar and nuclear genomes
    Jesse D Woodson
    Plant Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, California 92037, USA
    Nat Rev Genet 9:383-95. 2008
    ..Here we review recent advances in elucidating the intracellular signalling pathways that coordinate gene expression between organelles and the nucleus, with a focus on photosynthetic plants...
  39. ncbi Driving ribosome assembly
    Dieter Kressler
    Biochemie Zentrum der Universitat Heidelberg, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany
    Biochim Biophys Acta 1803:673-83. 2010
    ..This review summarizes our current knowledge on eukaryotic ribosome assembly, with particular focus on the molecular role of the involved energy-consuming enzymes...
  40. ncbi Pre-mRNA splicing: awash in a sea of proteins
    Melissa S Jurica
    Howard Hughes Medical Institute, Department of Biochemistry, Brandeis University, 415 South Street, Waltham, MA 02454, USA
    Mol Cell 12:5-14. 2003
    ..Another recurring theme regards the dynamic nature of spliceosomal complexes, which may be even more intricate than previously thought...
  41. ncbi Actin dynamics control SRF activity by regulation of its coactivator MAL
    Francesc Miralles
    Transcription Laboratory, Room 401, Cancer Research UK London Research Institute, Lincolns Inn Fields Laboratories, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
    Cell 113:329-42. 2003
    ..Constitutively cytoplasmic MAL derivatives interfere with MAL redistribution and Rho-actin signaling to SRF. MAL associates with several SRF target promoters regulated via the Rho-actin pathway...
  42. ncbi IkappaB kinase promotes tumorigenesis through inhibition of forkhead FOXO3a
    Mickey C T Hu
    Department of Molecular and Cellular Oncology, The University of Texas, Houston, TX 77030, USA
    Cell 117:225-37. 2004
    ..These results suggest the negative regulation of FOXO factors by IKK as a key mechanism for promoting cell growth and tumorigenesis...
  43. pmc Linear arrays of nuclear envelope proteins harness retrograde actin flow for nuclear movement
    G W Gant Luxton
    Department of Pathology and Cell Biology, Columbia University, New York, NY 10032, USA
    Science 329:956-9. 2010
  44. ncbi New insights into TGF-beta-Smad signalling
    Peter Ten Dijke
    Division of Cellular Biochemistry, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    Trends Biochem Sci 29:265-73. 2004
  45. ncbi Mediation of poly(ADP-ribose) polymerase-1-dependent cell death by apoptosis-inducing factor
    Seong Woon Yu
    Department of Neurology and Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA
    Science 297:259-63. 2002
    ..These data support a model in which PARP-1 activation signals AIF release from mitochondria, resulting in a caspase-independent pathway of programmed cell death...
  46. ncbi A calcineurin-dependent transcriptional pathway for cardiac hypertrophy
    J D Molkentin
    Department of Molecular Biology and Oncology, The University of Texas Southwestern Medical Center at Dallas, 75225 9148, USA
    Cell 93:215-28. 1998
    ..Pharmacologic inhibition of calcineurin activity blocks hypertrophy in vivo and in vitro. These results define a novel hypertrophic signaling pathway and suggest pharmacologic approaches to prevent cardiac hypertrophy and heart failure...
  47. pmc Evolutionary analysis of Arabidopsis, cyanobacterial, and chloroplast genomes reveals plastid phylogeny and thousands of cyanobacterial genes in the nucleus
    William Martin
    Institut fur Botanik, Heinrich Heine Universitat, Universitatsstrasse 1, 40225 Dusseldorf, Germany
    Proc Natl Acad Sci U S A 99:12246-51. 2002
  48. ncbi Dual regulation of Snail by GSK-3beta-mediated phosphorylation in control of epithelial-mesenchymal transition
    Binhua P Zhou
    Department of Molecular and Cellular Oncology, Breast Cancer Basic Research Program, The University of Texas M D Anderson Cancer Center, Houston, Texas 77030, USA
    Nat Cell Biol 6:931-40. 2004
    ..Furthermore, inhibition of GSK-3beta results in the upregulation of Snail and downregulation of E-cadherin in vivo. Thus, Snail and GSK-3beta together function as a molecular switch for many signalling pathways that lead to EMT...
  49. pmc "Cotranscriptionality": the transcription elongation complex as a nexus for nuclear transactions
    Roberto Perales
    Department of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, UCHSC, MS8101, P O Box 6511, Aurora CO, 80045, USA
    Mol Cell 36:178-91. 2009
    ..From this perspective, TECs can be viewed as potent "community organizers" within the nucleus...
  50. ncbi Reconstruction of zebrafish early embryonic development by scanned light sheet microscopy
    Philipp J Keller
    Cell Biology and Biophysics Unit, European Molecular Biology Laboratory EMBL, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    Science 322:1065-9. 2008
    ..We further derive a model of germ layer formation and show that the mesendoderm forms from one-third of the embryo's cells in a single event. Our digital embryos, with 55 million nucleus entries, are provided as a resource...
  51. pmc Altered nuclear retention of mRNAs containing inverted repeats in human embryonic stem cells: functional role of a nuclear noncoding RNA
    Ling Ling Chen
    Department of Genetics and Developmental Biology, University of Connecticut Stem Cell Institute, University of Connecticut Health Center, Farmington, CT 06030 3301, USA
    Mol Cell 35:467-78. 2009
    ..Taken together, these results assign a biological function to a large noncoding nuclear RNA in the regulation of mRNA export...
  52. pmc Visualization of the intracellular behavior of HIV in living cells
    David McDonald
    Dept of Microbiology and Immunology, University of Illinois, 835 South Wolcott Avenue, Chicago, IL 60612, USA
    J Cell Biol 159:441-52. 2002
    ..Based on these observations, we propose that HIV uses dynein and the microtubule network to facilitate the delivery of the viral genome to the nucleus of the cell during early postentry steps of the HIV life cycle...
  53. ncbi Failure of MBNL1-dependent post-natal splicing transitions in myotonic dystrophy
    Xiaoyan Lin
    Department of Neuroscience, University of Rochester Medical Center, Rochester, NY 14642, USA
    Hum Mol Genet 15:2087-97. 2006
    ..Sequestration of MBNL1, and failure to maintain these splicing transitions, has a pivotal role in the pathogenesis of muscle disease in DM...
  54. ncbi Nuclear actin regulates dynamic subcellular localization and activity of the SRF cofactor MAL
    Maria K Vartiainen
    Transcription Laboratory, Cancer Research UK, London Research Institute, Lincoln s Inn Fields Laboratories, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
    Science 316:1749-52. 2007
    ..Fluorescence resonance energy transfer analysis demonstrates that the MAL-actin interaction responds to extracellular signals. Serum-induced signaling is thus communicated to nuclear actin to control a transcriptional regulator...
  55. ncbi Transcriptional regulatory circuits controlling mitochondrial biogenesis and function
    Daniel P Kelly
    Center for Cardiovascula Research, Departments of Medicine, Molecular Biology and Pharmacology, and Pediatrics, Washington University School of Medicine, St Louis, Missouri 63119, USA
    Genes Dev 18:357-68. 2004
  56. ncbi Formation of neuronal intranuclear inclusions underlies the neurological dysfunction in mice transgenic for the HD mutation
    S W Davies
    Department of Anatomy and Developmental Biology, University College London, United Kingdom
    Cell 90:537-48. 1997
    ..The appearance in transgenic mice of these inclusions, followed by characteristic morphological change within neuronal nuclei, is strikingly similar to nuclear abnormalities observed in biopsy material from HD patients...
  57. pmc Exportin 5 is a RanGTP-dependent dsRNA-binding protein that mediates nuclear export of pre-miRNAs
    Markus T Bohnsack
    Zentrum fur Molekulare Biologie, Heidelberg, Germany
    RNA 10:185-91. 2004
    ..We further show that exportin 5 interacts with double-stranded RNA in a sequence-independent manner...
  58. pmc MEN epsilon/beta nuclear-retained non-coding RNAs are up-regulated upon muscle differentiation and are essential components of paraspeckles
    Hongjae Sunwoo
    Molecular and Cellular Biology Graduate Program, Stony Brook University, Stony Brook, New York 11794, USA
    Genome Res 19:347-59. 2009
    ..Our findings indicate that the MEN epsilon/beta non-coding RNAs are essential structural/organizational components of paraspeckles...
  59. ncbi Dynamic genome architecture in the nuclear space: regulation of gene expression in three dimensions
    Christian Lanctôt
    Department Biologie II, Ludwig Maximilians Universitat, Grosshadernerstr 2, Planegg Martinsried, Germany
    Nat Rev Genet 8:104-15. 2007
    ..Understanding how the dynamic nature of the positioning of genetic material in the nuclear space and the higher-order architecture of the nucleus are integrated is therefore essential to our overall understanding of gene regulation...
  60. ncbi The LIM domain: from the cytoskeleton to the nucleus
    Julie L Kadrmas
    Huntsman Cancer Institute and the Department of Biology, University of Utah, 2000 East, Circle of Hope, Salt Lake City, Utah 84112, USA
    Nat Rev Mol Cell Biol 5:920-31. 2004
    ..An emerging theme is that LIM proteins might function as biosensors that mediate communication between the cytosolic and the nuclear compartments...
  61. pmc Identification and characterization of a family of mammalian methyl-CpG binding proteins
    B Hendrich
    Institute of Cell and Molecular Biology, University of Edinburgh, Edinburgh EH9 3JR, Scotland
    Mol Cell Biol 18:6538-47. 1998
    ..The data demonstrate that MBD2 and MBD4 bind specifically to methyl-CpG in vitro and in vivo and are therefore likely to be mediators of the biological consequences of the methylation signal...
  62. ncbi Studies of nuclear and cytoplasmic behaviour during the five mitotic cycles that precede gastrulation in Drosophila embryogenesis
    V E Foe
    J Cell Sci 61:31-70. 1983
    ..This highly organized cytoplasm may be involved in providing positional information for the important process of nuclear determination that is known to occur during these stages...
  63. pmc Chromosome territories
    Thomas Cremer
    Biozentrum, Department of Biology II, Ludwig Maximilians University, Grosshadernerstrasse 2, 82152 Martinsried, Germany
    Cold Spring Harb Perspect Biol 2:a003889. 2010
    ..The article concludes with a discussion of open questions and new experimental strategies to answer them...
  64. pmc A screen for nuclear transcripts identifies two linked noncoding RNAs associated with SC35 splicing domains
    John N Hutchinson
    Center for Human Genetic Research, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA
    BMC Genomics 8:39. 2007
    ..To search for large noncoding RNAs that might control gene expression or mRNA metabolism, we used Affymetrix expression arrays to identify polyadenylated RNA transcripts displaying nuclear enrichment...
  65. pmc Exo- and endoribonucleolytic activities of yeast cytoplasmic and nuclear RNA exosomes are dependent on the noncatalytic core and central channel
    Elizabeth V Wasmuth
    Structural Biology Program, Sloan Kettering Institute, New York, NY 10065, USA
    Mol Cell 48:133-44. 2012
    ..These results suggest the exosome core and central channel is essential because it modulates each of the known RNase activities of the yeast RNA exosome...
  66. ncbi Regulation of the Caenorhabditis elegans longevity protein DAF-16 by insulin/IGF-1 and germline signaling
    K Lin
    Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, California, USA
    Nat Genet 28:139-45. 2001
    ..Together these findings reveal unexpected complexity in the DAF-16-dependent pathways that regulate aging...
  67. pmc Nuclear lamins
    Thomas Dechat
    Department of Cell and Molecular Biology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois 60611, USA
    Cold Spring Harb Perspect Biol 2:a000547. 2010
    ..They may also regulate chromatin functions by interacting with factors that epigenetically modify the chromatin or directly regulate replication or transcription...
  68. pmc The nuclear pore complex and nuclear transport
    Susan R Wente
    Department of Cell and Developmental Biology, Vanderbilt University Medical Center, Nashville, Tennessee 37232, USA
    Cold Spring Harb Perspect Biol 2:a000562. 2010
    ..All these requirements demonstrate the complex design of the NPC and the integral role it plays in key cellular processes...
  69. ncbi Mitochondrial retrograde signaling
    Zhengchang Liu
    Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9148, USA
    Annu Rev Genet 40:159-85. 2006
    ..Here we review advances in the yeast retrograde signaling pathway and highlight its regulatory factors and regulatory mechanisms, its physiological functions, and its connection to nutrient sensing, TOR signaling, and aging...
  70. ncbi Homeodomain-interacting protein kinase-2 phosphorylates p53 at Ser 46 and mediates apoptosis
    Gabriella D'Orazi
    Molecular Oncogenesis Laboratory, Regina Elena Cancer Institute, Via delle Messi d Oro 156, 00158 Rome, Italy
    Nat Cell Biol 4:11-9. 2002
    ..These data define a new functional interaction between p53 and HIPK2 that results in the targeted subcellular localization of p53 and initiation of apoptosis...
  71. ncbi The IkappaB-NF-kappaB signaling module: temporal control and selective gene activation
    Alexander Hoffmann
    Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA
    Science 298:1241-5. 2002
    ..Bimodal signal-processing characteristics with respect to stimulus duration are revealed by the model and are shown to generate specificity in gene expression...
  72. ncbi Neuronal MeCP2 is expressed at near histone-octamer levels and globally alters the chromatin state
    Peter J Skene
    Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh EH9 3JR, UK
    Mol Cell 37:457-68. 2010
    ..Our data argue that MeCP2 may not act as a gene-specific transcriptional repressor in neurons, but might instead dampen transcriptional noise genome-wide in a DNA methylation-dependent manner...
  73. pmc Export and stability of naturally intronless mRNAs require specific coding region sequences and the TREX mRNA export complex
    Haixin Lei
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 108:17985-90. 2011
    ..We conclude that naturally intronless mRNAs contain specific sequences that result in efficient packaging into the TREX mRNA export complex, thereby supplanting the splicing requirement for efficient mRNA export...
  74. ncbi A selective block of nuclear actin export stabilizes the giant nuclei of Xenopus oocytes
    Markus T Bohnsack
    Zentrum für Molekulare Biologie der Universität Heidelberg ZMBH, INF 282, D 69120 Heidelberg, Germany
    Nat Cell Biol 8:257-63. 2006
    ..Apparently, their mechanical integrity cannot be maintained by chromatin and the associated nuclear matrix, but instead requires an intranuclear actin-scaffold...
  75. ncbi Evolution of mitochondrial gene content: gene loss and transfer to the nucleus
    Keith L Adams
    Department of Botany, Iowa State University, 353 Bessey Hall, Ames, IA 50011, USA
    Mol Phylogenet Evol 29:380-95. 2003
    ..Several hypotheses have been proposed for why mitochondrial genes are transferred to the nucleus, why mitochondria retain genomes, and why functional gene transfer is almost exclusively unidirectional...
  76. ncbi Post-translational modifications regulating the activity and function of the nuclear factor kappa B pathway
    N D Perkins
    Division of Gene Regulation and Expression, University of Dundee, Dundee, Scotland, UK
    Oncogene 25:6717-30. 2006
  77. pmc Phosphorylation of beta-catenin by AKT promotes beta-catenin transcriptional activity
    Dexing Fang
    Brain Tumor Center and Department of Neuro Oncology, The University of Texas M D Anderson Cancer Center, Houston, Texas 77030, USA
    J Biol Chem 282:11221-9. 2007
    ..Phosphorylation of beta-catenin by AKT increases its transcriptional activity and promotes tumor cell invasion, indicating that AKT-dependent regulation of beta-catenin plays a critical role in tumor invasion and development...
  78. pmc Nuclear trafficking of the epidermal growth factor receptor family membrane proteins
    Y N Wang
    Department of Molecular and Cellular Oncology, University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA
    Oncogene 29:3997-4006. 2010
    ..Understanding the trafficking mechanisms as to how RTKs are transported from the cell surface to the nucleus will significantly contribute to understanding the functions of the nuclear RTKs...
  79. ncbi Dynamic FoxO transcription factors
    Haojie Huang
    Cancer Center and Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, MN 55455, USA
    J Cell Sci 120:2479-87. 2007
    ..Thus, the potent functions of FoxO proteins are tightly controlled by complex signaling pathways under physiological conditions; dysregulation of these proteins may ultimately lead to disease such as cancer...
  80. ncbi The genome of the diatom Thalassiosira pseudonana: ecology, evolution, and metabolism
    E Virginia Armbrust
    School of Oceanography, University of Washington, Seattle, WA 98195, USA
    Science 306:79-86. 2004
  81. pmc Interactions of mitochondrial and nuclear genes that affect male gametophyte development
    Maureen R Hanson
    Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14853 0001, USA
    Plant Cell 16:S154-69. 2004
  82. ncbi Duration of nuclear NF-kappaB action regulated by reversible acetylation
    - Chen Lf
    Gladstone Institute of Virology and Immunology, Department of Medicine, University of California, San Francisco, CA 94141, USA
    Science 293:1653-7. 2001
  83. ncbi Viable offspring derived from fetal and adult mammalian cells
    I Wilmut
    Roslin Institute Edinburgh, Roslin, Midlothian, UK
    Nature 385:810-3. 1997
  84. ncbi A hexanucleotide element directs microRNA nuclear import
    Hun Way Hwang
    Program in Human Genetics and Molecular Biology, McKusick Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Science 315:97-100. 2007
    ..Our results indicate that miRNAs sharing common 5' sequences, considered to be largely redundant, might have distinct functions because of the influence of cis-acting regulatory motifs...
  85. pmc Broadly sampled multigene analyses yield a well-resolved eukaryotic tree of life
    Laura Wegener Parfrey
    Program in Organismic and Evolutionary Biology, University of Massachusetts, 611 North Pleasant Street, Amherst, MA 01003, USA
    Syst Biol 59:518-33. 2010
  86. ncbi Genomic footprints of a cryptic plastid endosymbiosis in diatoms
    Ahmed Moustafa
    Interdisciplinary Program in Genetics, University of Iowa, Iowa City, IA 52242, USA
    Science 324:1724-6. 2009
    ..Chromalveolates appear to have recruited genes from the two major existing algal groups to forge a highly successful, species-rich protist lineage...
  87. ncbi NF-kappa B controls expression of inhibitor I kappa B alpha: evidence for an inducible autoregulatory pathway
    S C Sun
    Gladstone Institute of Virology and Immunology, University of California, San Francisco
    Science 259:1912-5. 1993
    ..Together, these results show that NF-kappa B controls the expression of I kappa B alpha by means of an inducible autoregulatory pathway...
  88. pmc Bringing KASH under the SUN: the many faces of nucleo-cytoskeletal connections
    David Razafsky
    Department of Ophthalmology and Visual Sciences, Washington University School of Medicine, St Louis, MO 63110, USA
    J Cell Biol 186:461-72. 2009
    ..Moreover, we discuss the function of these complexes in nuclear, centrosomal, and chromosome dynamics, and their connection to human disease...
  89. pmc Piecemeal microautophagy of nucleus in Saccharomyces cerevisiae
    Paul Roberts
    Department of Biology, University of Rochester, Rochester, New York 14627, USA
    Mol Biol Cell 14:129-41. 2003
    ..These results introduce a novel mode of selective microautophagy that targets nonessential components of the yeast nucleus for degradation and recycling in the vacuole...
  90. pmc Ubiquitination regulates PTEN nuclear import and tumor suppression
    Lloyd C Trotman
    Cancer Biology and Genetics Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    Cell 128:141-56. 2007
  91. pmc A mechanism of repression of TGFbeta/ Smad signaling by oncogenic Ras
    M Kretzschmar
    Cell Biology Program and Howard Hughes Medical Institute, Memorial Sloan Kettering Cancer Center, New York, New York 10021 USA
    Genes Dev 13:804-16. 1999
    ..Our results suggest a mechanism for the counterbalanced regulation of Smad2/Smad3 by TGFbeta and Ras signals in normal cells, and for the silencing of antimitogenic TGFbeta functions by hyperactive Ras in cancer cells...
  92. pmc Nuclear neighborhoods and gene expression
    Rui Zhao
    Molecular and Cellular Biology Program, Stony Brook University, Stony Brook, NY 11794, USA
    Curr Opin Genet Dev 19:172-9. 2009
    ..We are still at a very early, yet exciting stage in our journey toward deciphering the mechanism(s) that govern(s) the permissiveness of gene expression/repression within different nuclear neighborhoods...
  93. pmc Paraspeckles: nuclear bodies built on long noncoding RNA
    Charles S Bond
    School of Biomedical, Biomolecular, and Chemical Sciences, University of Western Australia, Crawley, Western Australia 6009, Australia
    J Cell Biol 186:637-44. 2009
    ..Given the large numbers of long noncoding transcripts currently being discovered through whole transcriptome analysis, paraspeckles may be a paradigm for a class of subnuclear bodies formed around long noncoding RNA...
  94. pmc Plasmodium falciparum heterochromatin protein 1 binds to tri-methylated histone 3 lysine 9 and is linked to mutually exclusive expression of var genes
    Karla Pérez-Toledo
    Departamento de Biomedicina Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional IPN, Apartado Postal 14 740, 07360, Mexico D F, Mexico
    Nucleic Acids Res 37:2596-606. 2009
    ..This is the first report implicating an HP1 protein in the control of antigenic variation of a protozoan parasite...
  95. ncbi IkappaB kinases phosphorylate NF-kappaB p65 subunit on serine 536 in the transactivation domain
    H Sakurai
    Discovery Research Laboratory, Tanabe Seiyaku Co, Ltd, 16 89 Kashima 3 chome, Yodogawa ku, Osaka 532 8505, Japan
    J Biol Chem 274:30353-6. 1999
    ..Furthermore, activation of IKKs by NF-kappaB-inducing kinase induced phosphorylation of p65 in vivo. Our finding, together with previous observations, suggests dual roles for IKK complex in the regulation of NF-kappaB.IkappaB complex...
  96. pmc Molecular poltergeists: mitochondrial DNA copies (numts) in sequenced nuclear genomes
    Einat Hazkani-Covo
    National Evolutionary Synthesis Center, Durham, North Carolina, USA
    PLoS Genet 6:e1000834. 2010
    ..Polymorphic numts in humans link maternally inherited mitochondrial genotypes to nuclear DNA haplotypes during the past, offering new opportunities to associate nuclear markers with mitochondrial markers back in time...
  97. ncbi The layered structure of human mitochondrial DNA nucleoids
    Daniel F Bogenhagen
    Department of Pharmacological Sciences, State University of New York at Stony Brook, Stony Brook, New York 11794 8651, USA
    J Biol Chem 283:3665-75. 2008
    ..These results are discussed in relation to a model for a layered structure of mtDNA nucleoids in which replication and transcription occur in the central core, whereas translation and complex assembly may occur in the peripheral region...
  98. ncbi Diversity of LEF/TCF action in development and disease
    L Arce
    Department of Microbiology and Molecular Genetics, University of California, Irvine, CA 92697 4025, USA
    Oncogene 25:7492-504. 2006
    ..Recent work from numerous laboratories has highlighted how this complexity has important biological consequences in development and disease...
  99. pmc Tumor suppressor p53 binding protein 1 (53BP1) is involved in DNA damage-signaling pathways
    I Rappold
    Division of Oncology Research, Mayo Clinic, Rochester, Minnesota 55905, USA
    J Cell Biol 153:613-20. 2001
    ..Third, 53BP1 is readily phosphorylated by ATM in vitro. Taken together, these results suggest that 53BP1 is an ATM substrate that is involved early in the DNA damage-signaling pathways in mammalian cells...
  100. pmc Acetylation of RelA at discrete sites regulates distinct nuclear functions of NF-kappaB
    Lin Feng Chen
    Gladstone Institute of Virology and Immunology, University of California at San Francisco, San Francisco, CA 94141, USA
    EMBO J 21:6539-48. 2002
    ..Together, these findings highlight how site-specific acetylation of RelA differentially regulates distinct biological activities of the NF-kappaB transcription factor complex...
  101. ncbi Mechanotransduction at a distance: mechanically coupling the extracellular matrix with the nucleus
    Ning Wang
    Department of Mechanical Science and Engineering, University of Illinois at Urbana Champaign, 1206 West Green Street, Urbana, Illinois 61801, USA
    Nat Rev Mol Cell Biol 10:75-82. 2009
    ..Here, we explore the molecular mechanisms by which forces might act at a distance to induce mechanochemical conversion in the nucleus and alter gene activities...

Research Grants72

  1. Lung Lysyl Oxidase Regulation by Metal Ion Homeostasis
    Wande Li; Fiscal Year: 2013
    ..elastin and histone H1, essential for organization and stabilization of the extracellular matrix (ECM) and the cell nucleus. This enzyme has been identified as a tumor suppressor, for example, inhibiting transforming activity of ras, a ..
  2. Interplay Between Tissue Architecture and Nuclear Organization in the DNA Damage
    Pierre Alexandre Vidi; Fiscal Year: 2013
    ..The proposed research will test this model from two angles: the cell nucleus and the cell-BM interphase. Aim 1 will be to characterize the role of NuMA in the DDR...
  3. Neuropeptide regulation of neurohypophyseal function
    Francis W Flynn; Fiscal Year: 2012
    ..NK3R expressed by VP magnocellular neurons were shown to be translocated from the cell membrane to the cell nucleus by confocal microscopy, immuno-electron microscopy and Western blot...
    Peter B Dervan; Fiscal Year: 2013
    ..of DNA binding proteins, inhibit DNA binding of a broad range of transcription factors, localize to the cell nucleus, bind to chromatin, and down regulate endogenous gene expression in cell culture...
  5. Hepatocyte Nox proteins in the pathogenesis of hepatocellular carcinoma
    Jinah Choi; Fiscal Year: 2013
    ..a direct oncogenic potential and increases the generation of hydroxyl radical and peroxynitrite close to the cell nucleus, inflicting DNA damage that can be mutagenic in the development of cancer...
  6. Intravenous Protein Therapy for Myotonic Dystrophy Type 1
    Richard Weisbart; Fiscal Year: 2010
    ..3E10 is a murine-derived monoclonal antibody that penetrates living cells and localizes to the cell nucleus without apparent injury to target cells (22, 23)...
    Katherine L Wilson; Fiscal Year: 2012
    ..Aim 3 is a whole-genome and promoter-specific analysis of BAF function in C. elegans, which is expected to identify genes relevant to its roles in muscle and DNA- damage responses. ..
  8. Live cell imaging of IgH and c-Myc gene loci and the role of nuclear organization
    Mark T Groudine; Fiscal Year: 2010
    ..The cell nucleus is a highly ordered organelle...
  9. Regulation of ENaC Transcription
    Bruce C Kone; Fiscal Year: 2013
    ..This proposal examines a novel network of interacting proteins in the cell nucleus that regulate transcription, the initial molecular step in generating this protein.
  10. Does the extreme variability of methadone metabolism have an epigenetic cause?
    Gavin R Schnitzler; Fiscal Year: 2010
  11. The role of the nucleoporin Nup98 in gene regulation
    Martin W Hetzer; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Eukaryotic gene expression is regulated at multiple levels in the cell nucleus, from histone modification and chromatin compaction to the synthesis, processing and export of mRNA...
    David J Stewart; Fiscal Year: 2011
    ..together with specialized proteins in structures called chromosomes that are themselves packed within the cell nucleus. How these chromosomes are duplicated, and how the DNA they contain is replicated, to allow faithful copies to ..
  13. Affinity Tagging of Adipocyte Nuclei to Examine Epigenetic Controls in Obesity
    Richard Brian Meagher; Fiscal Year: 2013
  14. Histone proline isomerization and gene regulation
    Nobuaki Kikyo; Fiscal Year: 2010
    ..In addition, the results will facilitate the progress of nuclear reprogramming and regenerative medicine, especially creation of patients-derived rejection-free tissues for the purpose of transplantation. ..
  15. Sox10 Alleles for Functional Analysis of Glial Lineages
    E Michelle Southard-Smith; Fiscal Year: 2013
    ..These first generaton tools have been valuable but have not permitted concurrent imaging of cell nucleus, morphology, or signaling between individual cells...
  16. Oxidative DNA base damage and repair at telomeres and the relevance to cell senes
    PATRICIA LYNN OPRESKO; Fiscal Year: 2013
    ..to targeted oxidative damage in telomeric and non-telomeric genomic regions, with 3D resolution in a single cell nucleus. We will measure the mobilization kinetics of various GFP-tagged BER proteins, and the protein domains required ..
    David M Knipe; Fiscal Year: 2013
    ..goals of this research are to define 1) the mechanisms by which herpes simplex virus (HSV) remodels the host cell nucleus to optimize its replication and 2) the functions and mechanisms of action of the HSV infected cell protein 8 (..
    Jerome F Strauss; Fiscal Year: 2010
    ..a testis-specific promoter, 35 kDa PF20s, which contains the C-terminus of PF20I, but is localized to the germ cell nucleus. PF20I is produced meiotically, PF20s accumulates post-meiotically...
  19. Mechanism of Apoptosis and Inhibition of Tumor Progression and Metastasis by Par-
    Vivek M Rangnekar; Fiscal Year: 2012
    ..Although ectopic Par-4 and the SAC domain have long been thought to effect apoptosis by acting within the cell nucleus to inhibit NF-kappaB, our recent transient transfection studies unexpectedly displayed apoptosis in neighboring ..
  20. Mapping serotonin receptors in lower urinary tract innervation
    E Michelle Southard-Smith; Fiscal Year: 2013
    ..The information gained will advance future studies of chronic visceral pain in the lower urinary tract and thus meet the criteria for inclusion in the Nociceptive GenitoUrinary Development Molecular Anatomy Project (nGUDMAP). ..
    Richard N Day; Fiscal Year: 2012
    ..In the pituitary cell nucleus, the homeodomain (HD) transcription factor Pit-1 orchestrates the activities of a network of regulatory ..
  22. Spinocerebellar ataxia 7 protein function.
    Patrick A Grant; Fiscal Year: 2010
    DESCRIPTION (provided by applicant): In the eukaryotic cell nucleus, DMA is packaged by histones into nucleosomes, the repeating subunits of chromatin...
  23. Release and trafficking of JC polyomavirus genomes and implications in AIDS
    ..our understanding of JCV pathogenesis, by studying exposure and delivery of the viral genome to the host cell nucleus. Since JCV must balance between protecting its viral genome and allowing the host replication machinery to ..
  24. Rescue of developmental disorders in utero by gene-specific small molecules
    Michael J Higgins; Fiscal Year: 2011
    ..with specificities and affinities comparable to transcription factors (TF), are cell permeable, localize to the cell nucleus, and even bind in the context of chromatin...
  25. The Fat1 Cadherin in Atherosclerotic Vascular Disease
    NICHOLAS E SIBINGA; Fiscal Year: 2013
    ..We have found that this Fat1 fragment accumulates in the cell nucleus, where it co-operates with other factors to activate genes important for maintenance of mature vascular smooth ..
  26. Accelerating Cancer Research with Single Cell Arrays
    Heinz Ulrich Weier; Fiscal Year: 2009
    ..This will open new avenues for the analysis of small samples like those obtained by fine needle biopsies as well as the analysis of circulating or exfoliated tumor cells. ..
  27. Applying nanosecond pulse electric fields to treat skin cancer
    RICHARD LEE NUCCITELLI; Fiscal Year: 2011
    ..Two targets that we have identified are the cell nucleus and the tumor's blood supply...
  28. The role of DNA damage response signaling in chronic kidney disease
    Rannar Airik; Fiscal Year: 2013
    ..The BCH/HMS has several collaborative research groups working on kidney development and diseases providing an ideal environment for my training and career development. ..
    Jay C Brown; Fiscal Year: 2012
    ..The capsid, which contains the virus DNA, is assembled in the infected cell nucleus. A DNA-free capsid shell is first formed and later packaged with DNA...
  30. The Nuclear Pore Complex: Interphase and Mitotic Function
    Katharine S Ullman; Fiscal Year: 2012
    ..When this coordination is disrupted, the cell nucleus does not reform properly...
    Joseph G Gall; Fiscal Year: 2013
    ..by applicant): The long-term goal of our research is to understand the functional organization of the animal cell nucleus. Specifically, we concentrate on two molecular processes that take place in the nucleus: the synthesis of RNA ..
  32. Vertebrate Non-Coding RNPs: Integrating function, biogenesis and survival
    Joan A Steitz; Fiscal Year: 2013
    ..and protein interactions that underlie the biogenesis and stability of ncRNAs, particularly in the vertebrate cell nucleus. Specifically, a novel chaperone function for a scaRNA in assembling the U2 snRNP will be investigated...
  33. Nuclear Export of Nuclear Hormone Receptors
    Lizabeth A Allison; Fiscal Year: 2010
    ..PUBLIC HEALTH RELEVANCE: Trafficking of regulatory proteins into the cell nucleus, where genetic information is stored and processed, is fundamental to the organization and functioning of all ..
  34. Mechanisms in Polyoma and Papillomavirus Assembly
    ROBERT GARCEA; Fiscal Year: 2013
    ..We also hypothesize that specific locations within the cell nucleus, the PML-nuclear bodies, are the site of this interaction and subsequent virus assembly...
  35. Molecular Mechanism of Hepadnavirus Persistence
    Jianming Hu; Fiscal Year: 2012
    ..the episomal viral DNA, the so-called covalently closed circular (CCC) DNA, persists in the infected cell nucleus even after years of antiviral treatment...
    Carolyn J Anderson; Fiscal Year: 2012
    ..to basic questions regarding the effect of p53 status on cell killing by receptor-mediated targeted radiotherapy, as well as questions regarding basic biochemistry of p53-mediated copper trafficking to the cell nucleus.
  37. Phosphorylation and Functions of the RNA Polymerase CTD
    Arno L Greenleaf; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): We are investigating mechanisms by which different activities in the cell nucleus are connected to the transcription apparatus via interactions with the hyper-phosphorylated C-terminal repeat domain (..
  38. Scanning Correlation Microscopy Methods for Quantifying DNA Repair Kinetics
    Georgios Alexandrakis; Fiscal Year: 2012
    ..fluctuations in image pixels, to quantify the repair kinetics of proteins with sparse accumulation in the cell nucleus. We will use RICS to quantify the repair kinetics of the DNA-dependent protein kinase catalytic subunit (DNA-..
  39. Early events in infection of epithelial cells with Epstein-Barr Virus
    SARAH M VALENCIA; Fiscal Year: 2011
    ..Understanding how EBV gets into the epithelial cell nucleus and the events that it triggers along the way is important for design of strategies to interrupt or ameliorate ..
  40. Methods Development for CVM Cell Migration Study in Drosophila
    ANGELIKE M STATHOPOULOS; Fiscal Year: 2013
    ..Development of the gastrointestinal tract of animals is highly similar, so studies of how CVM cell migration influences gut formation in Drosophila have the potential to have far-reaching insight into human development. ..
    David F Stern; Fiscal Year: 2013
    ..Aim 3 will evaluate unique features of cell regulation by ErbB4 forms that act in the cell nucleus. This affords ErbB4 the ability to reprogram major cell activities, some relevant to cancer...
  42. Effects of nuclear PTHrP on bone formation and the skeletal microenvironment
    Blake Eason Hildreth; Fiscal Year: 2011
    ..Unlike PTH, PTHrP has a region called the NLS, which takes the protein to the cell nucleus and may contribute to PTHrP's more widespread effects...
  43. Genetic and Molecular Approach to Identify Ooplasm Reprogramming Factors
    Keith E Latham; Fiscal Year: 2010
    ..We will combine these data here to identify gene mapping, gene expression, and functional studies those specific genes that determine oocyte reprogramming capacity, and hence serve as key oocyte reprogramming factors. ..
  44. Transcriptional control of the mouse aA-crystallin locus
    Ales Cvekl; Fiscal Year: 2013
  45. Oocyte factors for reprogramming to pluripotency
    RENEE A contact REIJO PERA; Fiscal Year: 2010
    ..We build on a substantial preliminary data set that allows us to immediately progress on all aims simultaneously through further funding and staffing of the proposed research. ..
    Kenneth S Zaret; Fiscal Year: 2013
    ..factors and revealed the basis by which Fox proteins, compared to other factors, scan the genome in the living cell nucleus. In addition, we discovered a dynamic signaling network that induces the hepatic fate In multipotent endoderm, ..
  47. Nano-architecture Based Biomarkers for Cervical Cancer Screening
    Yang Liu; Fiscal Year: 2013
  48. Development of Small Molecule Crm-1 Inhibitor for Pancreatic Cancer Therapy
    Ramzi M Mohammad; Fiscal Year: 2013
    ..findings demonstrate that SINE (KPT-185 and clinical candidate KPT-251) can lock Par-4 in pancreatic cancer cell nucleus by inhibiting CRM-1 that leads to cancer cell selective apoptosis and anti-tumor effects...
  49. Inhibitors of Kaposi???s Sarcoma Herpesvirus
    Kenneth M Kaye; Fiscal Year: 2011
    ..This work will develop tests to identify compounds that can be used to study this virus. These compounds may potentially be used to prevent and treat the cancers this virus causes. ..
  50. New Gene Vectors for Cystic Fibrosis (CF): Extra- & Intracellular Barriers
    JUSTIN S HANES; Fiscal Year: 2010
    ..epithelial cells following incubation in CF mucus;(iii) efficient and active accumulation around and in the cell nucleus within minutes of cell entry;and (iv) significantly improved gene expression in cell culture and in CF mouse ..
  51. Investigating the mechanism of ferritin protection of DNA
    ANNA RUTH ARNOLD; Fiscal Year: 2013
  52. Neuroprotection by a Nuclear Carbonic Anhydrase in C. elegans
    KEITH W NEHRKE; Fiscal Year: 2013
    ..Our preliminary data demonstrate that the nematode C. elegans expresses a CA that selectively localizes to the cell nucleus, is induced by hypoxia, and when lost results in neurodegeneration (ie dysfunction followed by cell death)...
  53. Dissecting the Molecular Mechanisms of Canonical Wnt Signaling
    Raymond Habas; Fiscal Year: 2013
    ..these new components will help to define the molecular nature of Wnt signaling from the plasma membrane to the cell nucleus and ultimately lead to a better understanding of how deregulated Wnt signaling results in cancer formation...
  54. Tegument ICP0 and HSV Entry
    Anthony V Nicola; Fiscal Year: 2010
    ..goal of this project is to understand the virus-cell interactions that HSV uses to gain access to the host cell nucleus during the earliest stages of infection...
  55. Investigations of Mouse Interspecies Hybrids
    James M McGrath; Fiscal Year: 2013
    ..of how early events after fertilization program the zygote genome and as such will inform epigenetic gene regulation, transposon regulation, the reprogramming of a somatic cell nucleus in animal cloning, and evolution/speciation.
  56. Artificial site-specific RNA endonuclease as new Myotonic Dystrophy therapeutics
    Zefeng Wang; Fiscal Year: 2012
    ..We will design novel ASREs that can specifically bind and cleave expanding RNA repeats in the cell nucleus where the toxic RNAs are accumulated...
  57. Targeting and Function of Golgi Membrane Proteins
    Carolyn E Machamer; Fiscal Year: 2011
    ..has an unusual structure consisting of sets of stacked cisternal membranes gathered into a ribbon near the cell nucleus. The function of this elaborate structure is not known...
  58. Control of nuclear thyroid hormone signaling by cell surface proteins
    DANIEL RICHARD BUCHHOLZ; Fiscal Year: 2010
    ..hormone (TH) is critical for growth, development, and metabolism, and works by binding TH receptors in the cell nucleus and changing gene expression...
  59. Regulatory RNAs as mediators and biomarkers in Alzheimer's Disease
    CLAES ROBERT WAHLESTEDT; Fiscal Year: 2012
    ..BACE1-AS is enriched in the cell nucleus but upon exposure to amyloid-[unreadable] 1-42 (A[unreadable] 1-42), or other cellular stressors, this ..
  60. Adenylate Kinase in Energetics of Cell Nucleus and Heart Regeneration
    Petras P Dzeja; Fiscal Year: 2013
    ..Aim #2 Molecular mechanisms of cell cycle dependent translocation of AK isoforms to the cell nucleus and association with mitotic spindles and cytokinesis machinery and the role in integration of mitochondrial ..
  61. Cyclin-Dependent Kinases in Oncogenesis
    Peter Sicinski; Fiscal Year: 2013
    ..by applicant): The proliferation of mammalian cells is driven by the core cell cycle machinery operating in cell nucleus. Cyclin-dependent kinases (CDKs) represent key components of this machinery...
  62. Molecular and Cellular Pathogenesis of Emery-Dreifuss Muscular Dystrophy
    JASON CHEOL CHOI; Fiscal Year: 2012
    ..Understanding how defective proteins of the cell nucleus lead to EDMD-associated heart muscle damage will provide the knowledge necessary to devise an effective ..
    MARGARET GARNER; Fiscal Year: 2001
    ..Na,K-ATPases are also present in cell nucleus and endoplasmic reticulum of cultured lens epithelial cells and in the cell nucleus and ER of rat liver...
    Lee Chin; Fiscal Year: 1991
    ..The dosimetric tools developed here will be appropriate for later application to clinical radio-immunotherapy...
  65. Defective nuclear FGFR1 signaling - a new model for PD
    MICHAL STACHOWIAK; Fiscal Year: 2003
    ..In the Snc the most abundant are the HMW non-released isoforms of FGF-2 that localize almost exclusively in the cell nucleus. Functions of nuclear FGF-2 and the significance of its depletion in Snc neurons in PD patients remain unknown...
    Mario Stevenson; Fiscal Year: 2001
    ..property of HIV-1 is governed by the ability of the reverse transcription complex to translocate to the host cell nucleus in the absence of mitosis...
    Jay Brown; Fiscal Year: 2007
    ..The capsid, which contains the virus DMA, is assembled in the infected cell nucleus. A DNA-free capsid shell is first formed and later packaged with DNA...
  68. Misregulation of apoptosis in cloned pig embryos
    Heide Schatten; Fiscal Year: 2007
    ..1% in pigs) for which causes are only little understood. Incomplete or abnormal remodeling of the donor cell nucleus following transfer, resulting in imprinting failures and abnormal gene expression throughout development, are ..
  69. Proteomic Characterization of IC Bladder
    Pradeep Tyagi; Fiscal Year: 2006
    ..The nuclear matrix is the structural scaffolding of the cell nucleus and plays a central role in the regulation of important cellular processes...
    Stephen Rice; Fiscal Year: 2003
    ..of mammalian cells with herpes simplex virus type 1 (HSV-1) results in dramatic alterations to the host cell nucleus, so that viral genes are expressed at high levels while cellular genes are nearly completely suppressed...
  71. Biomaterials that Promote Healing & Prevent Infection
    JAMES BRYERS; Fiscal Year: 2004
    ..processes are initiated and controlled by a communication pathway from stimulus, to cell surface receptor, to cell nucleus, to mRNA, to cytokine signaling agents and higher tissue response...
    KENNETH STERLING; Fiscal Year: 1980
    ..has not previously been reported, leading to the supposition that saturable receptors exist only in the cell nucleus. The present work shows for the first time that in vivo displacement of tracer (125I)-T3 is regularly observed ..