sarcolemma

Summary

Summary: The excitable plasma membrane of a muscle cell. (Glick, Glossary of Biochemistry and Molecular Biology, 1990)

Top Publications

  1. ncbi Dysferlin interacts with affixin (beta-parvin) at the sarcolemma
    Chie Matsuda
    Research Institute of Neurobiology, Neuroscience Research Institute, AIST, Central 6, Tsukuba, Ibaraki, Japan
    J Neuropathol Exp Neurol 64:334-40. 2005
  2. pmc Animal models for muscular dystrophy show different patterns of sarcolemmal disruption
    V Straub
    Department of, Howard Hughes Medical Institute, University of Iowa College of Medicine, Iowa City, Iowa 52242, USA
    J Cell Biol 139:375-85. 1997
  3. pmc Role of sarcolemmal K(ATP) channels in cardioprotection against ischemia/reperfusion injury in mice
    Masashi Suzuki
    Department of Pharmacology, Chiba University Graduate School of Medicine, Chiba, Japan
    J Clin Invest 109:509-16. 2002
  4. ncbi Association of dystrophin-related protein with dystrophin-associated proteins in mdx mouse muscle
    K Matsumura
    Howard Hughes Medical Institute, University of Iowa College of Medicine, Iowa City 52242
    Nature 360:588-91. 1992
  5. pmc Loss of T-tubules and other changes to surface topography in ventricular myocytes from failing human and rat heart
    Alexander R Lyon
    Department of Cardiac Medicine, National Heart and Lung Institute, Imperial College, London SW3 6LY, United Kingdom
    Proc Natl Acad Sci U S A 106:6854-9. 2009
  6. pmc Obscurin is required for ankyrinB-dependent dystrophin localization and sarcolemma integrity
    Davide Randazzo
    Molecular Medicine Section, Department of Molecular and Developmental Medicine, University of Siena, 53100 Siena, Italy
    J Cell Biol 200:523-36. 2013
  7. pmc Plectin 1f scaffolding at the sarcolemma of dystrophic (mdx) muscle fibers through multiple interactions with beta-dystroglycan
    Günther A Rezniczek
    Max F Perutz Laboratories, Department of Molecular Cell Biology, University of Vienna, A 1030 Vienna, Austria
    J Cell Biol 176:965-77. 2007
  8. pmc Novel mini-dystrophin gene dual adeno-associated virus vectors restore neuronal nitric oxide synthase expression at the sarcolemma
    Yadong Zhang
    Department of Molecular Microbiology and Immunology, University of Missouri, Columbia, MO 65212, USA
    Hum Gene Ther 23:98-103. 2012
  9. pmc NO production results in suspension-induced muscle atrophy through dislocation of neuronal NOS
    Naoki Suzuki
    Department of Molecular Therapy, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Kodaira, Tokyo, Japan
    J Clin Invest 117:2468-76. 2007
  10. pmc Elevated subsarcolemmal Ca2+ in mdx mouse skeletal muscle fibers detected with Ca2+-activated K+ channels
    N Mallouk
    Laboratoire de Physiologie des Elements Excitables, Unite Mixte de Recherche, Centre National de la Recherche Scientifique 5578, Universite Claude Bernard Lyon I, 43 Boulevard du 11 Novembre 1918, 69622 Villeurbanne Cedex, France
    Proc Natl Acad Sci U S A 97:4950-5. 2000

Detail Information

Publications333 found, 100 shown here

  1. ncbi Dysferlin interacts with affixin (beta-parvin) at the sarcolemma
    Chie Matsuda
    Research Institute of Neurobiology, Neuroscience Research Institute, AIST, Central 6, Tsukuba, Ibaraki, Japan
    J Neuropathol Exp Neurol 64:334-40. 2005
    ..Here we show that affixin is a dysferlin binding protein that colocalizes with dysferlin at the sarcolemma of normal human skeletal muscle...
  2. pmc Animal models for muscular dystrophy show different patterns of sarcolemmal disruption
    V Straub
    Department of, Howard Hughes Medical Institute, University of Iowa College of Medicine, Iowa City, Iowa 52242, USA
    J Cell Biol 139:375-85. 1997
    ..One hypothesis is that the DGC protects the sarcolemma from contraction-induced damage...
  3. pmc Role of sarcolemmal K(ATP) channels in cardioprotection against ischemia/reperfusion injury in mice
    Masashi Suzuki
    Department of Pharmacology, Chiba University Graduate School of Medicine, Chiba, Japan
    J Clin Invest 109:509-16. 2002
    ..The rapid heart rate of the mouse (>600 beats per minute) may magnify the relative importance of sarcK(ATP) channels during ischemia, prompting caution in the extrapolation of the conclusions to larger mammals...
  4. ncbi Association of dystrophin-related protein with dystrophin-associated proteins in mdx mouse muscle
    K Matsumura
    Howard Hughes Medical Institute, University of Iowa College of Medicine, Iowa City 52242
    Nature 360:588-91. 1992
    ..to a dramatic reduction of the dystrophin-associated proteins (156DAG, 59DAP, 50DAG, 43DAG and 35DAG) in the sarcolemma of patients with Duchenne muscular dystrophy and mdx mice...
  5. pmc Loss of T-tubules and other changes to surface topography in ventricular myocytes from failing human and rat heart
    Alexander R Lyon
    Department of Cardiac Medicine, National Heart and Lung Institute, Imperial College, London SW3 6LY, United Kingdom
    Proc Natl Acad Sci U S A 106:6854-9. 2009
    T-tubular invaginations of the sarcolemma of ventricular cardiomyocytes contain junctional structures functionally coupling L-type calcium channels to the sarcoplasmic reticulum calcium-release channels (the ryanodine receptors), and ..
  6. pmc Obscurin is required for ankyrinB-dependent dystrophin localization and sarcolemma integrity
    Davide Randazzo
    Molecular Medicine Section, Department of Molecular and Developmental Medicine, University of Siena, 53100 Siena, Italy
    J Cell Biol 200:523-36. 2013
    ....
  7. pmc Plectin 1f scaffolding at the sarcolemma of dystrophic (mdx) muscle fibers through multiple interactions with beta-dystroglycan
    Günther A Rezniczek
    Max F Perutz Laboratories, Department of Molecular Cell Biology, University of Vienna, A 1030 Vienna, Austria
    J Cell Biol 176:965-77. 2007
    In skeletal muscle, the cytolinker plectin is prominently expressed at Z-disks and the sarcolemma. Alternative splicing of plectin transcripts gives rise to more than eight protein isoforms differing only in small N-terminal sequences (5-..
  8. pmc Novel mini-dystrophin gene dual adeno-associated virus vectors restore neuronal nitric oxide synthase expression at the sarcolemma
    Yadong Zhang
    Department of Molecular Microbiology and Immunology, University of Missouri, Columbia, MO 65212, USA
    Hum Gene Ther 23:98-103. 2012
    ..Localization of nNOS to the sarcolemma may greatly improve the therapeutic outcome in DMD (Lai, Y., Thomas, G.D., Yue, Y., et al. [2009]. J. Clin...
  9. pmc NO production results in suspension-induced muscle atrophy through dislocation of neuronal NOS
    Naoki Suzuki
    Department of Molecular Therapy, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Kodaira, Tokyo, Japan
    J Clin Invest 117:2468-76. 2007
    ..Among several DGC members, only neuronal NOS (nNOS) quickly dislocated from the sarcolemma to the cytoplasm during tail suspension...
  10. pmc Elevated subsarcolemmal Ca2+ in mdx mouse skeletal muscle fibers detected with Ca2+-activated K+ channels
    N Mallouk
    Laboratoire de Physiologie des Elements Excitables, Unite Mixte de Recherche, Centre National de la Recherche Scientifique 5578, Universite Claude Bernard Lyon I, 43 Boulevard du 11 Novembre 1918, 69622 Villeurbanne Cedex, France
    Proc Natl Acad Sci U S A 97:4950-5. 2000
    ..These data favor the hypothesis according to which an increased calcium entry is associated with the absence of dystrophin in mdx skeletal muscle, leading to Ca(2+) overload at the subsarcolemmal level...
  11. pmc Sarcolemma-localized nNOS is required to maintain activity after mild exercise
    Yvonne M Kobayashi
    Howard Hughes Medical Institute, University of Iowa, Roy J and Lucille A Carver College of Medicine, 4283 Carver Biomedical Research Building, 285 Newton Road, Iowa City, Iowa 52242 1101, USA
    Nature 456:511-5. 2008
    ..that this exaggerated fatigue response is distinct from a loss in specific force production by muscle, and that sarcolemma-localized signalling by neuronal nitric oxide synthase (nNOS) in skeletal muscle is required to maintain ..
  12. ncbi The dystrophin glycoprotein complex: signaling strength and integrity for the sarcolemma
    Karen A Lapidos
    Department of Molecular Genetics and Cell Biology, University of Chicago, Ill 60637, USA
    Circ Res 94:1023-31. 2004
    ..Vascular reactivity is altered in response to primary degeneration in striated myocytes and arises from a vascular smooth muscle cell-extrinsic mechanism...
  13. ncbi Primary structure of dystrophin-associated glycoproteins linking dystrophin to the extracellular matrix
    O Ibraghimov-Beskrovnaya
    Howard Hughes Medical Institute, University of Iowa College of Medicine, Iowa City 52242
    Nature 355:696-702. 1992
    ..Thus, the 156K DAG is a new laminin-binding glycoprotein which may provide a linkage between the sarcolemma and extracellular matrix...
  14. pmc Dystrophins carrying spectrin-like repeats 16 and 17 anchor nNOS to the sarcolemma and enhance exercise performance in a mouse model of muscular dystrophy
    Yi Lai
    Department of Molecular Microbiology and Immunology, University of Missouri, One Hospital Drive, Columbia, MO 65212, USA
    J Clin Invest 119:624-35. 2009
    b>Sarcolemma-associated neuronal NOS (nNOS) plays a critical role in normal muscle physiology...
  15. ncbi From T-tubule to sarcolemma: damage-induced dysferlin translocation in early myogenesis
    Lars Klinge
    Institute of Human Genetics, University of Newcastle upon Tyne, International Centre for Life, Central Pkwy, NE1 3BZ Newcastle upon Tyne, England, UK
    FASEB J 21:1768-76. 2007
    ..In mature skeletal muscle, dysferlin is located predominantly at the sarcolemma, where it plays a role in membrane fusion and repair...
  16. ncbi Absence of the skeletal muscle sarcolemma chloride channel ClC-1 in myotonic mice
    C A Gurnett
    Howard Hughes Medical Institute, Department of Physiology and Biophysics, University of Iowa, College of Medicine, Iowa City 52242, USA
    J Biol Chem 270:9035-8. 1995
    ..Immunolocalization shows prominent ClC-1 antigen in the sarcolemma of both type I and II muscle fibers...
  17. pmc MG53 nucleates assembly of cell membrane repair machinery
    Chuanxi Cai
    Department of Physiology and Biophysics, Robert Wood Johnson Medical School, 675 Hoes Lane, Piscataway, NJ 08854, USA
    Nat Cell Biol 11:56-64. 2009
    ....
  18. pmc Crucial role of the small GTPase Rac1 in insulin-stimulated translocation of glucose transporter 4 to the mouse skeletal muscle sarcolemma
    Shuji Ueda
    Division of Molecular Biology, Department of Biochemistry and Molecular Biology, Kobe University Graduate School of Medicine, 7 5 1 Kusunoki cho, Chuo Ku, Kobe 650 0017, Japan
    FASEB J 24:2254-61. 2010
    ..translocation of the facilitative glucose transporter GLUT4 from its storage sites to the skeletal muscle sarcolemma depends on Rac1...
  19. ncbi Fibre-type specific concentration of focal adhesion kinase at the sarcolemma: influence of fibre innervation and regeneration
    Martin Fluck
    M E Müller Institute for Biomechanics, University of Bern, Bühlestrasse 26, 3000 Bern 9, Switzerland
    J Exp Biol 205:2337-48. 2002
    ..a sarcolemmal protein complex that enables lateral transfer of forces and ensures the stability of the sarcolemma. The present investigation tested whether localisation of a major assembly factor of FACs, focal adhesion kinase ..
  20. ncbi Null mutation in the desmin gene gives rise to a cardiomyopathy
    L Thornell
    Department of Anatomy, Umea University, Umea, Sweden
    J Mol Cell Cardiol 29:2107-24. 1997
    ..At the ultrastructural level changes in the intercalated discs, disruption of the sarcolemma and supercontraction of myofibrils seemed to be the key events leading to cardiomyocyte death...
  21. ncbi Dystroglycan: an extracellular matrix receptor linked to the cytoskeleton
    M D Henry
    Howard Hughes Medical Institute, Department of Physiology, University of Iowa College of Medicine, 400 Eckstein Medical Research Building, Iowa City, IA 5224, 2 USA
    Curr Opin Cell Biol 8:625-31. 1996
    ....
  22. ncbi In vivo imaging of molecular interactions at damaged sarcolemma
    Urmas Roostalu
    Institute of Toxicology and Genetics, Karlsruhe Institute of Technology and University of Heidelberg, Eggenstein Leopoldshafen, Germany
    Dev Cell 22:515-29. 2012
    ..Thus, sarcolemmal repair relies on the ordered assembly of a protein-membrane scaffold. Moreover, we provide several lines of evidence that the membrane for sarcolemmal repair is derived from a specialized plasma membrane compartment...
  23. ncbi Decrease in resting calcium and calcium entry associated with slow-to-fast transition in unloaded rat soleus muscle
    Bodvael Fraysse
    Sezione di Farmacologia, Dipartimento Farmaco Biologico, Universita degli Studi di Bari, Via Orabona 4 Campus, 70125, Bari, Italy
    FASEB J 17:1916-8. 2003
    ..According to the crucial role of resting cytosolic free calcium in the maintenance and the adaptation of muscle phenotype, our results suggest that slow-to-fast transition of HU soleus muscle is calcium dependent...
  24. pmc Compartmentation of membrane processes and nucleotide dynamics in diffusion-restricted cardiac cell microenvironment
    Alexey E Alekseev
    Marriott Heart Disease Research Program, Division of Cardiovascular Diseases, Mayo Clinic, Rochester, MN, USA
    J Mol Cell Cardiol 52:401-9. 2012
    ..Compared to substrate diffusion in the cytosol, diffusional restrictions underneath the sarcolemma are much larger and could impede ion and nucleotide movement by a factor of 10(3)-10(5)...
  25. pmc Ankyrin-B regulates Kir6.2 membrane expression and function in heart
    Jingdong Li
    Division of Cardiovascular Medicine, Department of Internal Medicine, University of Iowa Carver College of Medicine, Iowa City, Iowa 52242, USA
    J Biol Chem 285:28723-30. 2010
    ..Collectively, our new findings define a new role for cardiac ankyrin polypeptides in regulation of ion channel membrane expression in heart...
  26. pmc Consequences of cardiac myocyte-specific ablation of KATP channels in transgenic mice expressing dominant negative Kir6 subunits
    Xiaoyong Tong
    Pediatric Cardiology, NYU School of Medicine, 560 First Ave, TCH 521, New York, NY 10016, USA
    Am J Physiol Heart Circ Physiol 291:H543-51. 2006
    ....
  27. ncbi Localization of alpha 7 integrins and dystrophin suggests potential for both lateral and longitudinal transmission of tension in large mammalian muscles
    Angelika C Paul
    Department of Anatomy and Structural Biology, University of Otago, Dunedin, New Zealand
    Cell Tissue Res 308:255-65. 2002
    ..at junctional sites, alpha 7B integrin and dystrophin were also expressed ubiquitously along the non-junctional sarcolemma, suggesting potential involvement in diffuse lateral transmission of tension between adjacent fibres...
  28. ncbi Desmin and actin alterations in human muscles affected by delayed onset muscle soreness: a high resolution immunocytochemical study
    Ji Guo Yu
    Department of Integrative Medical Biology, Section for Anatomy, Umea University, 901 87 Umea, Sweden
    Histochem Cell Biol 118:171-9. 2002
    ..7% (SD 3.9%) and 11.4% (SD 4.6%), respectively. We suggest that the increased staining of actin and desmin reflects an increased synthesis of these proteins as part of an adaptation process following the unaccustomed eccentric exercise...
  29. pmc Progressive muscular dystrophy in alpha-sarcoglycan-deficient mice
    F Duclos
    Howard Hughes Medical Institute, Department of Physiology and Biophysics and Department of Neurology, University of Iowa College of Medicine, Iowa City, Iowa 52242 1101, USA
    J Cell Biol 142:1461-71. 1998
    ..that the sarcoglycan-sarcospan complex is requisite for stable association of alpha-dystroglycan with the sarcolemma. The Sgca-deficient mice will be a valuable model for elucidating the pathogenesis of sarcoglycan deficient limb-..
  30. pmc Biglycan recruits utrophin to the sarcolemma and counters dystrophic pathology in mdx mice
    Alison R Amenta
    Department of Neuroscience, Brown University, Providence, RI 02912, USA
    Proc Natl Acad Sci U S A 108:762-7. 2011
    ..Systemically delivered rhBGN up-regulates utrophin at the sarcolemma and reduces muscle pathology in the mdx mouse model of DMD...
  31. ncbi Autosomal recessive limb-girdle muscular dystrophy, LGMD2F, is caused by a mutation in the delta-sarcoglycan gene
    V Nigro
    Istituto di Patologia Generale e Oncologia, Facolta di Medicina, Seconda Universita degli Studi di Napoli, Italy
    Nat Genet 14:195-8. 1996
    ..We now show that a homozygous mutation in the delta SG gene (a single nucleotide deletion that alters its reading frame) is the cause of LGMD2F...
  32. pmc Myogenic Akt signaling upregulates the utrophin-glycoprotein complex and promotes sarcolemma stability in muscular dystrophy
    Angela K Peter
    Department of Physiological Science, University of California, Los Angeles, CA 90095, USA
    Hum Mol Genet 18:318-27. 2009
    Duchenne muscular dystrophy is caused by dystrophin mutations that lead to structural instability of the sarcolemma membrane, myofiber degeneration/regeneration and progressive muscle wasting...
  33. pmc Predetermined recruitment of calcium release sites underlies excitation-contraction coupling in rat atrial myocytes
    L Mackenzie
    Laboratory of Molecular Signalling, The Babraham Institute, Babraham, Cambridge CB2 4AT, UK
    J Physiol 530:417-29. 2001
    ..eager sites suggests that they reflect clusters of RyRs closely coupled to voltage-operated Ca2+ channels in the sarcolemma. Furthermore, their propensity to show spontaneous Ca2+ sparks is consistent with an intrinsically higher ..
  34. ncbi Exercise induces a cardiac mitochondrial phenotype that resists apoptotic stimuli
    Andreas N Kavazis
    Department of Applied Physiology and Kinesiology, University of Florida, Gainesville, FL 32611, USA
    Am J Physiol Heart Circ Physiol 294:H928-35. 2008
    ..Furthermore, these results are consistent with the concept that exercise-induced mitochondrial adaptations contribute to exercise-induced cardioprotection...
  35. ncbi Expression of caveolin-3 in skeletal, cardiac, and smooth muscle cells. Caveolin-3 is a component of the sarcolemma and co-fractionates with dystrophin and dystrophin-associated glycoproteins
    K S Song
    Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142 1479, USA
    J Biol Chem 271:15160-5. 1996
    ..Immunolocalization of caveolin-3 in skeletal muscle fibers demonstrates that caveolin-3 is localized to the sarcolemma (muscle cell plasma membrane) and coincides with the distribution of another muscle-specific plasma membrane ..
  36. ncbi Skeletal muscle disuse induces fibre type-dependent enhancement of Na(+) channel expression
    J F Desaphy
    Unit of Pharmacology, Department of Pharmaco-Biology, School of Pharmacy, University of Bari, via Orabona, 4 campus, 70125 Bari, Italy
    Brain 124:1100-13. 2001
    ..The observed increase in sodium current density may reduce the resistance to fatigue of antigravity muscle fibres, an effect that may contribute to muscle impairment in humans after space flight or after long immobilization...
  37. ncbi Plectin tethers desmin intermediate filaments onto subsarcolemmal dense plaques containing dystrophin and vinculin
    Takao Hijikata
    Department of Anatomy, Gunma University School of Medicine, 3 39 22 Showa machi, 371 8511 Maebashi, Japan
    Histochem Cell Biol 119:109-23. 2003
    ..To better understand the functional roles of plectin beneath the sarcolemma of skeletal muscles and to gain some insights into the underlying mechanism of plectin-deficient muscular ..
  38. ncbi Disrupted mechanical stability of the dystrophin-glycoprotein complex causes severe muscular dystrophy in sarcospan transgenic mice
    Angela K Peter
    Department of Physiological Science, University of California, Los Angeles, CA 90095, USA
    J Cell Sci 120:996-1008. 2007
    ..Sarcospan transgenic muscle does not display sarcolemma damage, which is distinct from dystrophin- and sarcoglycan-deficient muscular dystrophies...
  39. ncbi The transverse-axial tubular system of cardiomyocytes
    C Ferrantini
    Division of Physiology, Department of Clinical and Experimental Medicine, University of Florence, Florence, Italy
    Cell Mol Life Sci 70:4695-710. 2013
    ..histological feature of striated muscle cells is the presence of deep invaginations of the plasma membrane (sarcolemma), most commonly referred to as T-tubules or the transverse-axial tubular system (TATS)...
  40. pmc Membrane targeting and stabilization of sarcospan is mediated by the sarcoglycan subcomplex
    R H Crosbie
    Howard Hughes Medical Institute, Department of Physiology and Biophysics, University of Iowa College of Medicine, Iowa City, Iowa 52242, USA
    J Cell Biol 145:153-65. 1999
    ..a unique tetraspan-like dystrophin-associated protein, which we have named sarcospan (SPN) for its multiple sarcolemma spanning domains (Crosbie, R.H., J. Heighway, D.P. Venzke, J.C. Lee, and K.P. Campbell. 1997. J. Biol. Chem...
  41. ncbi Dystrophin-glycoprotein complex and laminin colocalize to the sarcolemma and transverse tubules of cardiac muscle
    R Klietsch
    Department of Anatomy and Cell Biology, University of Toronto, Ontario, Canada
    Circ Res 72:349-60. 1993
    ..The DAPs and dystrophin codistributed not only in the free surface sarcolemma but also in interior regions of the myofibers where T tubules are present...
  42. ncbi Nitric oxide synthase complexed with dystrophin and absent from skeletal muscle sarcolemma in Duchenne muscular dystrophy
    J E Brenman
    Department of Physiology, University of California, San Francisco School of Medicine 94143 0444, USA
    Cell 82:743-52. 1995
    Nitric oxide (NO) is synthesized in skeletal muscle by neuronal-type NO synthase (nNOS), which is localized to sarcolemma of fast-twitch fibers. Synthesis of NO in active muscle opposes contractile force...
  43. ncbi Immunohistological intensity measurements as a tool to assess sarcolemma-associated protein expression
    V Arechavala-Gomeza
    The Dubowitz Neuromuscular Centre, Institute of Child Health, University College London, UK
    Neuropathol Appl Neurobiol 36:265-74. 2010
    ..We therefore sought to develop a method to quantify relative levels of sarcolemma-associated proteins using digitally captured images of immunolabelled sections of skeletal muscle.
  44. ncbi Myocardial ischemia selectively depletes cardiolipin in rabbit heart subsarcolemmal mitochondria
    E J Lesnefsky
    Division of Cardiology, Case Western Reserve University, Cleveland, Ohio 44106, USA
    Am J Physiol Heart Circ Physiol 280:H2770-8. 2001
    ..Cardiolipin content was preserved in IFM during ischemia. Thus cardiolipin is a relatively early target of ischemic mitochondrial damage, leading to loss of oxidative phosphorylation through cytochrome oxidase in SSM...
  45. ncbi Eccentric exercise-induced injuries to contractile and cytoskeletal muscle fibre components
    J Friden
    Department of Hand Surgery, Sahlgrenska University Hospital, Goteborg, Sweden
    Acta Physiol Scand 171:321-6. 2001
    ....
  46. pmc Dystrophin-glycoprotein complex is highly enriched in isolated skeletal muscle sarcolemma
    K Ohlendieck
    Howard Hughes Medical Institute, University of Iowa College of Medicine, Iowa City 52242
    J Cell Biol 112:135-48. 1991
    ..of rabbit skeletal muscle have been used as markers in the isolation and characterization of skeletal muscle sarcolemma membranes...
  47. ncbi Functional diversity of dystroglycan
    Manuela Bozzi
    Istituto di Biochimica e Biochimica Clinica, Universita Cattolica del Sacro Cuore, Largo F Vito 1, Rome, Italy
    Matrix Biol 28:179-87. 2009
    ....
  48. ncbi Neuronal NOS is dislocated during muscle atrophy in amyotrophic lateral sclerosis
    Naoki Suzuki
    Department of Neurology, Tohoku University School of Medicine, 1 1 Seiryo machi, Aoba ku, Sendai 980 8574, Japan
    J Neurol Sci 294:95-101. 2010
    ..with 100 mM NaCl and barely detected in the pellet fraction, suggesting fragile anchoring of nNOS to the sarcolemma. We also showed an elevated expression of atrogin-1, key molecules in muscle atrophy at the end stage...
  49. ncbi Reduced cell anchorage may cause sarcolemma-specific collagen VI deficiency in Ullrich disease
    G Kawahara
    Department of Neuromuscular Research, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Ogawahigashi cho, Kodaira, Tokyo, Japan
    Neurology 69:1043-9. 2007
    ..We previously reported that the majority of patients with UCMD have sarcolemma-specific collagen VI deficiency (SSCD)...
  50. pmc Soleus muscle in glycosylation-deficient muscular dystrophy is protected from contraction-induced injury
    Jessica D Gumerson
    Department of Molecular and Integrative Physiology, University of Michigan, Ann Arbor, Michigan 48109 0622, USA
    Am J Physiol Cell Physiol 299:C1430-40. 2010
    ..Interestingly, we show that soleus muscles have a markedly higher sarcolemma expression of β(1)-containing integrins compared with EDL and gastrocnemius muscles...
  51. ncbi Exercise and insulin increase muscle fatty acid uptake by recruiting putative fatty acid transporters to the sarcolemma
    Jan F C Glatz
    Department of Physiology, Cardiovascular Research Institute Maastricht CARIM, Maastricht University, Maastricht, The Netherlands
    Curr Opin Clin Nutr Metab Care 5:365-70. 2002
    ..The focus of this review is to describe newly obtained insights on the recruitment of fatty acid transporters and their malfunctioning in diabetes...
  52. ncbi Mass spectrometric identification of dystrophin isoform Dp427 by on-membrane digestion of sarcolemma from skeletal muscle
    Caroline Lewis
    Department of Biology, National University of Ireland, Maynooth, Co Kildare, Ireland
    Anal Biochem 404:197-203. 2010
    ..of certain target proteins, here we have applied direct on-membrane digestion of one-dimensional blots of the sarcolemma-enriched fraction and the isolated dystrophin-glycoprotein complex...
  53. pmc Sarcolemmal-restricted localization of functional ClC-1 channels in mouse skeletal muscle
    John D Lueck
    Department of Pharmacology and Physiology, and 2 Department of Neurology, University of Rochester, Rochester, NY 14642, USA
    J Gen Physiol 136:597-613. 2010
    ..and confocal microscopy, native ClC-1 channels in adult mouse FDB fibers were localized within the sarcolemma, 90 degrees out of phase with double rows of dihydropyridine receptor immunostaining of the T-tubule system...
  54. ncbi Identification of a novel sarcoglycan gene at 5q33 encoding a sarcolemmal 35 kDa glycoprotein
    V Nigro
    Istituto di Patologia Generale ed Oncologia, Facolta di Medicina, Seconda Universita degli Studi di Napoli, Italy
    Hum Mol Genet 5:1179-86. 1996
    ..The gene spans at least 100 kb and is composed of eight exons. The identification of a novel sarcoglycan component modifies the current model of the dystrophin-glycoprotein complex...
  55. pmc Organization of ryanodine receptors, transverse tubules, and sodium-calcium exchanger in rat myocytes
    Isuru D Jayasinghe
    Department of Physiology, School of Medical Sciences, University of Auckland, Auckland, New Zealand
    Biophys J 97:2664-73. 2009
    ..Distance maps for all colocalization pairs were calculated to give the distance between centroids of punctate labeling and edges for distributed components. The possible roles for punctate NCX labeling are discussed...
  56. pmc Changes in the organization of excitation-contraction coupling structures in failing human heart
    David J Crossman
    Department of Physiology, Faculty of Medicine and Health Sciences, University of Auckland, Auckland, New Zealand
    PLoS ONE 6:e17901. 2011
    ....
  57. ncbi Characterization of an extensive transverse tubular network in sheep atrial myocytes and its depletion in heart failure
    Katharine M Dibb
    Unit of Cardiac Physiology, University of Manchester, Core Technology Facility, 46 Grafton St, Manchester M13 9NT, United Kingdom
    Circ Heart Fail 2:482-9. 2009
    ..The aims of this study were to determine (i) whether atrial myocytes from a large mammal (sheep) possess t-tubules, (ii) whether these are functionally important, and (iii) whether they are disrupted in heart failure...
  58. ncbi Reconstituted human cardiac KATP channels: functional identity with the native channels from the sarcolemma of human ventricular cells
    A P Babenko
    Departments of Cell Biology and Medicine, Baylor College of Medicine, Houston, TX, USA
    Circ Res 83:1132-43. 1998
    ..The results suggest that reconstituted huSUR2A/huKIR6.2 channels represent KATP channels in sarcolemma of human cardiomyocytes and are an adequate experimental model with which to examine structure-function ..
  59. ncbi Effects of adenovirus-mediated sorcin overexpression on excitation-contraction coupling in isolated rabbit cardiomyocytes
    Tim Seidler
    Department of Cardiology and Pneumology, Georg August University Goettingen, Goettingen, Germany
    Circ Res 93:132-9. 2003
    ..The effect of sorcin overexpression on Ca2+ sparks indicates an effect on the ryanodine receptor that may also influence excitation-contraction coupling...
  60. ncbi Age-associated increases in oxidative stress and antioxidant enzyme activities in cardiac interfibrillar mitochondria: implications for the mitochondrial theory of aging
    Sharon Judge
    University of Florida, Biochemistry of Aging Laboratory, Gainesville, FL 32611, USA
    FASEB J 19:419-21. 2005
    ..Our results emphasize the importance of studying both mitochondrial populations when attempting to elucidate the contribution of mitochondrial dysfunction to myocardial aging...
  61. pmc Sarcolemmal ATP-sensitive K(+) channels control energy expenditure determining body weight
    Alexey E Alekseev
    Department of Medicine, Mayo Clinic, Rochester, MN 55905, USA
    Cell Metab 11:58-69. 2010
    ....
  62. ncbi Contractile function, sarcolemma integrity, and the loss of dystrophin after skeletal muscle eccentric contraction-induced injury
    Richard M Lovering
    Department of Physical Therapy and Rehabilitation Sciences, School of Medicine, University of Maryland, Baltimore 21201, USA
    Am J Physiol Cell Physiol 286:C230-8. 2004
    ..These data indicate the selective vulnerability of dystrophin after a single eccentric contraction-induced injury and suggest a critical role of dystrophin in force transduction...
  63. pmc A novel mechanism of myocyte degeneration involving the Ca2+-permeable growth factor-regulated channel
    Yuko Iwata
    Department of Molecular Physiology, National Cardiovascular Center Research Institute, Osaka, Japan
    J Cell Biol 161:957-67. 2003
    ..channel (GRC), which belongs to the transient receptor potential channel family, is elevated in the sarcolemma of skeletal and/or cardiac muscle in dystrophic human patients and animal models deficient in dystrophin or ..
  64. ncbi Differential susceptibility of subsarcolemmal and intermyofibrillar mitochondria to apoptotic stimuli
    Peter J Adhihetty
    School of Kinesiology and Health Science, York University, Farquharson Building, 302, Toronto, Ontario, Canada M3J 1P3
    Am J Physiol Cell Physiol 289:C994-C1001. 2005
    ....
  65. ncbi Change of chloride ion channel conductance is an early event of slow-to-fast fibre type transition during unloading-induced muscle disuse
    Sabata Pierno
    Sezione di Farmacologia, Dipartimento Farmaco Biologico, Facolta di Farmacia, Universita degli Studi di Bari, Italy
    Brain 125:1510-21. 2002
    ..is known about the effects of weightlessness on the resting membrane chloride conductance (gCl), which controls sarcolemma excitability and influences fibre type transition during development and adult life...
  66. pmc The zebrafish candyfloss mutant implicates extracellular matrix adhesion failure in laminin alpha2-deficient congenital muscular dystrophy
    Thomas E Hall
    The Victor Chang Cardiac Research Institute, 384 Victoria Street, Darlinghurst, Sydney NSW 2010, Australia
    Proc Natl Acad Sci U S A 104:7092-7. 2007
    ..Using these and other analyses, we present a model of lama2 function where fiber detachment external to the sarcolemma is mechanically induced, and retracted fibers with uncompromised membranes undergo subsequent apoptosis.
  67. ncbi Loss of sarcolemma nNOS in sarcoglycan-deficient muscle
    Rachelle H Crosbie
    Howard Hughes Medical Institute, Department of Physiology and Biophysics, University of Iowa College of Medicine, Iowa City, Iowa 52242, USA
    FASEB J 16:1786-91. 2002
    nNOS, anchored to the sarcolemma through its interactions with the dystrophin-glycoprotein complex, is dramatically reduced in dystrophin-deficient mdx mice and Duchenne muscular dystrophy patients...
  68. ncbi The Duchenne muscular dystrophy gene product is localized in sarcolemma of human skeletal muscle
    E E Zubrzycka-Gaarn
    Genetics Department, Hospital for Sick Children, Toronto, Ontario, Canada
    Nature 333:466-9. 1988
    ..derived from the N-terminal region of human DMD cDNA strongly react with an antigen present in skeletal muscle sarcolemma on cryostat sections of normal human muscle biopsies...
  69. ncbi The carboxyl-terminal region of ahnak provides a link between cardiac L-type Ca2+ channels and the actin-based cytoskeleton
    Annette Hohaus
    Max Delbruck Center for Molecular Medicine, 13092 Berlin, Germany
    FASEB J 16:1205-16. 2002
    ..Confocal microscopy of human left ventricular tissue localized the carboxyl-terminal ahnak portion to the sarcolemma including the T-tubular system and the intercalated disks of cardiomyocytes...
  70. ncbi Ankyrin-G in skeletal muscle: tissue-specific alternative splicing contributes to the complexity of the sarcolemmal cytoskeleton
    Alexander A Hopitzan
    Institut Jacques Monod CNRS Universités Paris VI et VII 2 Place Jussieu, F 75251 Paris Cedex 05, France
    Exp Cell Res 309:86-98. 2005
    ..Our results demonstrate the tissue-dependent alternative splicing of Ank3 in skeletal muscle and point to novel functions of small ankyrins-G in organizing microdomains of the plasma membrane...
  71. ncbi Branched fibres in old dystrophic mdx muscle are associated with mechanical weakening of the sarcolemma, abnormal Ca2+ transients and a breakdown of Ca2+ homeostasis during fatigue
    Stewart I Head
    Department of Physiology, School of Medical Sciences, University of New South Wales, Sydney, NSW 2052, Australia
    Exp Physiol 95:641-56. 2010
    ..In particular, any therapy aimed at rescuing the defective gene will presumably have to do so before the number of branched fibres has increased to a level where the muscle is mechanically compromised...
  72. pmc Enhanced apoptotic propensity in diabetic cardiac mitochondria: influence of subcellular spatial location
    Courtney L Williamson
    Division of Exercise Physiology and Center for Cardiovascular and Respiratory Sciences, West Virginia University School of Medicine, Morgantown, West Virginia, USA
    Am J Physiol Heart Circ Physiol 298:H633-42. 2010
    ..These results indicate that diabetes mellitus is associated with an enhanced apoptotic propensity in IFM, suggesting a differential apoptotic susceptibility of distinct mitochondrial subpopulations based upon subcellular location...
  73. ncbi Targeted expression of insulin-like growth factor-I reduces early myofiber necrosis in dystrophic mdx mice
    Thea Shavlakadze
    School of Anatomy and Human Biology, University of Western Australia, Crawley, Perth, Western Australia 6009, Australia
    Mol Ther 10:829-43. 2004
    ..results from defects in the subsarcolemmal protein dystrophin that cause membrane fragility and tears in the sarcolemma, and these lead to the destruction of the myofibers...
  74. pmc Correction of ClC-1 splicing eliminates chloride channelopathy and myotonia in mouse models of myotonic dystrophy
    Thurman M Wheeler
    Department of Neurology, University of Rochester, Rochester, New York 14618, USA
    J Clin Invest 117:3952-7. 2007
    ..splicing of the muscle-specific chloride channel (ClC-1) and reduced conductance of chloride ions in the sarcolemma. However, the mechanistic basis of the chloride channelopathy and its relationship to the development of ..
  75. ncbi Clustering of protein kinase A-dependent CFTR chloride channels in the sarcolemma of guinea-pig ventricular myocytes
    Andrew F James
    Department of Cell Physiology, National Institute for Physiological Sciences, Okazaki 444 8585, Japan
    Biochem Biophys Res Commun 391:841-5. 2010
    ..These results suggest that CFTR Cl(PKA) channels occur in clusters in the sarcolemma of guinea-pig ventricular myocytes; there was no evidence of a heterogeneous distribution of clusters between ..
  76. pmc Fatty acid binding protein facilitates sarcolemmal fatty acid transport but not mitochondrial oxidation in rat and human skeletal muscle
    Graham P Holloway
    Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, Canada, N1G 2W1
    J Physiol 582:393-405. 2007
    ....
  77. ncbi Transversal stiffness of fibers and desmin content in leg muscles of rats under gravitational unloading of various durations
    I V Ogneva
    State Scientific Center of Russian Federation Institute of Biomedical Problems of Russian Academy of Sciences, Moscow, Russia
    J Appl Physiol (1985) 109:1702-9. 2010
    The aim of this research was the analysis of structural changes in various parts of the sarcolemma and contractile apparatus of muscle fibers by measuring their transversal stiffness by atomic force microscopy under gravitational ..
  78. pmc In vivo requirement of the alpha-syntrophin PDZ domain for the sarcolemmal localization of nNOS and aquaporin-4
    M E Adams
    Department of Physiology and Biophysics, University of Washington, Seattle, WA 98195, USA
    J Cell Biol 155:113-22. 2001
    alpha-Syntrophin is a scaffolding adapter protein expressed primarily on the sarcolemma of skeletal muscle...
  79. ncbi Triacylglycerol accumulation in human obesity and type 2 diabetes is associated with increased rates of skeletal muscle fatty acid transport and increased sarcolemmal FAT/CD36
    Arend Bonen
    Department of Human Biology and Nutritional Sciences, University of Guelph, Guelph, Ontario, N1G 2W1, Canada
    FASEB J 18:1144-6. 2004
    ..e., GLUT4 is known to be retained in its intracellular depots while FAT/CD36 is retained at the sarcolemma. This redistribution of FAT/CD36 to the sarcolemma may contribute to the etiology of insulin resistance in human ..
  80. ncbi Intracellular localization of dysferlin and its association with the dihydropyridine receptor
    Beryl N Ampong
    Department of Molecular Therapy, National Institute of Neuroscience, National Center for Neurology and Psychiatry, Tokyo, Japan
    Acta Myol 24:134-44. 2005
    ..was proposed to have a putative functional role in mediating the fusion of intracellular vesicles to the sarcolemma during injury-induced membrane repair, but dysferlin has been found not only at the sarcolemma but also within ..
  81. ncbi Differential targeting of nNOS and AQP4 to dystrophin-deficient sarcolemma by membrane-directed alpha-dystrobrevin
    Marvin E Adams
    Department of Physiology and Biophysics, University of Washington, Seattle, WA 98195, USA
    J Cell Sci 121:48-54. 2008
    ..We used a transgenic approach to restore alpha-dystrobrevin to the sarcolemma in mice that lack dystrophin (mdx mice) to study two interrelated functions: (1) the ability of alpha-..
  82. ncbi Insulin and contraction-induced movement of fatty acid transport proteins to skeletal muscle transverse-tubules is distinctly different than to the sarcolemma
    Leslie E Stefanyk
    Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, ON, Canada N1G 2W1
    Metabolism 61:1518-22. 2012
    Fatty acid (FA) transport proteins are known to exist on the sarcolemma of skeletal muscle. However, it is unknown whether the t-tubules, which comprise ~60% of the cell surface, also harbor these proteins...
  83. ncbi Distribution of aquaporin 4 on sarcolemma of fast-twitch skeletal myofibres
    Mika Kaakinen
    Department of Anatomy and Cell Biology, University of Oulu, PO Box 5000, FIN 90014 Oulu, Finland
    Cell Tissue Res 329:529-39. 2007
    The aquaporin 4 (AQP4) water channel is present on the sarcolemma of fast-twitch-type skeletal myofibres...
  84. ncbi Functional consequences of detubulation of isolated rat ventricular myocytes
    Mark R Fowler
    School of Biomedical Sciences, University of Leeds, Worsley Building, Leeds, West Yorkshire, LS2 9NQ, UK
    Cardiovasc Res 62:529-37. 2004
    ..The aim of this study was to investigate the role of the t-tubules in the response of Ca(i) and contraction to interventions that alter the transsarcolemmal Na(+)gradient...
  85. pmc Translocation of the Na+/H+ exchanger 1 (NHE1) in cardiomyocyte responses to insulin and energy-status signalling
    Scott P Lawrence
    Department of Biology and Biochemistry, University of Bath, Claverton Down, Bath BA2 7AY, UK
    Biochem J 432:515-23. 2010
    ..However, we have examined in the present study whether a net translocation of NHE1 to the sarcolemma of cardiomyocytes may also be involved in the activation process...
  86. ncbi Human melanoma/NG2 chondroitin sulfate proteoglycan is expressed in the sarcolemma of postnatal human skeletal myofibers. Abnormal expression in merosin-negative and Duchenne muscular dystrophies
    Stefania Petrini
    Unit of Molecular Medicine, Bambino Gesù Hospital IRCCS, Rome, Italy
    Mol Cell Neurosci 23:219-31. 2003
    ..We show that MCSP/NG2 expression is detectable in the sarcolemma, and in the neuromuscular junction of human postnatal skeletal muscle, and it gradually reduces with advancing ..
  87. ncbi Skeletal muscle basement membrane-sarcolemma-cytoskeleton interaction minireview series
    Kevin P Campbell
    Howard Hughes Medical Institute and Department of Physiology, University of Iowa College of Medicine, Iowa City, Iowa 52242, USA
    J Biol Chem 278:12599-600. 2003
  88. pmc Re-evaluation of sarcolemma injury and muscle swelling in human skeletal muscles after eccentric exercise
    Ji Guo Yu
    Department of Surgical and Perioperative Sciences, Sports Medicine Unit, Umea University, Umea, Sweden
    PLoS ONE 8:e62056. 2013
    ..This study aimed to re-evaluate the paradigm of muscular alterations with regard to muscle sarcolemma integrity and fibre swelling in human muscles after voluntary eccentric exercise leading to DOMS...
  89. ncbi New methods for the isolation of skeletal muscle sarcolemma and sarcoplasmic reticulum allowing a comparison between the mammalian and amphibian beta(2)-adrenergic receptors and calcium pumps
    S J Hemmings
    Department of Physiology, College of Medicine, University of Saskatchewan, Saskatoon, Canada
    Cell Biochem Funct 19:133-41. 2001
    ....
  90. ncbi Modification of intracellular calcium concentration in cardiomyocytes by inhibition of sarcolemmal Na+/H+ exchanger
    Harjot K Saini
    Institute of Cardiovascular Sciences, St Boniface General Hospital Research Centre, University of Manitoba, Winnipeg, MB, Canada R2H 2A6
    Am J Physiol Heart Circ Physiol 291:H2790-800. 2006
    ....
  91. ncbi Imaging and elasticity measurements of the sarcolemma of fully differentiated skeletal muscle fibres
    Enrico Defranchi
    Department of Biophysical and Electronic Engineering, University of Genova, 16145 Genova, Italy
    Microsc Res Tech 67:27-35. 2005
    This study aimed to describe the three-dimensional structure and the elastic properties of the sarcolemma of adult, fully differentiated, skeletal muscle fibres combining Atomic Force Microscopy (AFM) and optical microscopy...
  92. ncbi Impact of repeated bouts of eccentric exercise on sarcolemma disruption in human skeletal muscle
    A Costa
    Semmelweis University Department of Biomechanics, Faculty of Physical Education and Sport Sciences, Budapest, Hungary
    Acta Physiol Hung 96:189-202. 2009
    In animal models, unaccustomed eccentric exercise (EE) has been widely related to muscle fiber membrane (sarcolemma) damage...
  93. ncbi Sarcolemma agonist-induced interactions between InsP3 and ryanodine receptors in Ca2+ oscillations and waves in smooth muscle
    J G McCarron
    Institute of Biomedical and Life Sciences, Neuroscience and Biomedical Systems, West Medical Building, University of Glasgow, Glasgow G12 8QQ, UK
    Biochem Soc Trans 31:920-4. 2003
    Smooth muscle cells respond to InsP(3)-generating (sarcolemma-acting) neurotransmitters and hormones by releasing Ca(2+) from the internal store...
  94. ncbi Plasma membrane removal in rat skeletal muscle fibers reveals caveolin-3 hot-spots at the necks of transverse tubules
    Robyn M Murphy
    Department of Zoology, La Trobe University, Victoria 3086, Australia
    Exp Cell Res 315:1015-28. 2009
    ..Skeletal muscle fibers present a model where the surface membrane (sarcolemma) can be completely separated from the cell by mechanical dissection...
  95. ncbi Eccentric contractions leading to DOMS do not cause loss of desmin nor fibre necrosis in human muscle
    Ji Guo Yu
    Department of Integrative Medical Biology, Section for Anatomy, Umea University, 901 87 Umea, Sweden
    Histochem Cell Biol 118:29-34. 2002
    ..In animal models of eccentric exercise-induced injury, disruption of the cytoskeleton and the sarcolemma of muscle fibres occurs within the first hour after the exercise, since a rapid loss of staining of desmin, a ..
  96. pmc Impaired recovery of dysferlin-null skeletal muscle after contraction-induced injury in vivo
    Joseph A Roche
    Department of Physiology, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
    Neuroreport 19:1579-84. 2008
    ..Our data indicate that recovery of control muscles from injury primarily involves sarcolemmal repair whereas recovery of dysferlin-null muscles primarily involves myogenesis without repair and long-term survival of myofibers...
  97. ncbi Ullrich disease due to deficiency of collagen VI in the sarcolemma
    H Ishikawa
    Department of Neuromuscular Research, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Kodaira, Tokyo
    Neurology 62:620-3. 2004
    ..eight patients with Ullrich disease in whom collagen VI was present in the interstitium but was absent from the sarcolemma. By electron microscopy, collagen VI in the interstitium was never linked to the basal lamina...
  98. ncbi Dysfunction of ionic channels in cardiomyocyte sarcolemma and cardiac arrhythmias
    V M Moroz
    Vinnitsa National University of Medicine
    Bull Exp Biol Med 141:397-9. 2006
    ..Intracellular imbalance of Na+ and Ca2+ ions can underlie the arrhythmogenic effects of antiarrhythmic drugs...
  99. pmc Sarcolemma instability during mechanical activity in Largemyd cardiac myocytes with loss of dystroglycan extracellular matrix receptor function
    Zhyldyz Kabaeva
    Department of Molecular and Integrative Physiology, University of Michigan, Ann Arbor, MI 48109 0622, USA
    Hum Mol Genet 20:3346-55. 2011
    ....
  100. ncbi Sarcolemma-specific autoantibodies in canine inflammatory myopathy
    Sabine Hankel
    The Burnham Institute, 10901 North Torrey Pines Road, La Jolla, CA 92037, USA
    Vet Immunol Immunopathol 113:1-10. 2006
    ..show that a high proportion of affected Boxers and Newfoundlands have circulating autoantibodies against unknown sarcolemma antigens, that are muscle-specific but not species specific...
  101. ncbi Protein kinase B/Akt phosphorylates and inhibits the cardiac Na+/H+ exchanger NHE1
    Andrew K Snabaitis
    Cardiovascular Division, King s College London, The Rayne Institute, St Thomas Hospital, London SE1 7EH, United Kingdom
    Circ Res 103:881-90. 2008
    ..Heterologously expressed myr-PKBalpha was present in the sarcolemma, colocalized with NHE1 at the intercalated disc regions, increased NHE1 phosphorylation, and reduced NHE1 ..

Research Grants65

  1. Excitation-contraction Coupling in Normal and Dystrophic Mammalian Muscle
    Julio L Vergara; Fiscal Year: 2013
    ..of representative DGC protein components in order to assess if they are associated exclusively with the sarcolemma or if they have a more ubiquitous distribution in association with the Z-line and the TTS...
  2. Generation of Wunen/LPP3-based therapy for muscular dystrophy
    Hannele Ruohola Baker; Fiscal Year: 2010
    ..in the same physical niche as mammalian muscle satellite stem cells which is between the basal lamina and the sarcolemma of existing muscle fibers...
  3. Dual AAV Vectors for Duchenne Muscular Dystrophy Therapy
    Dongsheng Duan; Fiscal Year: 2012
    ..can fully restore muscle force, this minigene cannot restore neuronal nitric oxide synthase (nNOS) to the sarcolemma. The loss of sarcolemmal nNOS leads to functional ischemia in mdx mice and DMD patients...
  4. Mechanisms of cardiac Ca dysregulation and arrhythmias in ankyrin B deficiency
    Sanda Despa; Fiscal Year: 2013
    ..directly relates to the inability of those mutants to target NCX and NKA correctly to the cardiac myocyte sarcolemma. Thus, altered NCX and NKA expression and membrane distribution are key to the cardiac phenotype generated by ..
  5. Myocardial Adenosine Receptors
    James G Dobson; Fiscal Year: 2010
    ..kinase C isoforms to translocate and bind to RACK (receptor activated C kinase) proteins of the myocardial sarcolemma. Also the involvement of p38-MAPK (mitogen activated protein kinase) in the A1 R-mediated antiadrenergic action ..
  6. Phenotypic characterization of nitric oxide regulation of cardiac function by MRI
    Xin Yu; Fiscal Year: 2013
    ..In the heart, nNOS has been reported to be associated with the sarcolemma, sarcoplasmic reticulum (SR), and mitochondria...
  7. Vesicle translocation and the metabolic syndrome
    JONATHAN BOGAN; Fiscal Year: 2013
    ..GLUT4 storage vesicles (GSVs), which fuse at the cell surface and insert GLUT4 glucose transporters into the sarcolemma. The differential targeting of GLUT4 in basal and insulin-stimulated cells determines insulin responsiveness...
  8. Signaling Mechanisms of the Dystrophin-Glycoprotein Complex
    Andrea Arnett; Fiscal Year: 2012
    ..16 has no direct, mechanical link to the actin cytoskeleton, but can still assemble and stabilize the DGC at the sarcolemma. Thus, Dpi 16 should permit functional and structural studies of both the C-terminal portion of dystrophin and ..
  9. Improving Cardiac Regeneration Using Stem Cells with Enhanced Calcium Influx
    Sharven Taghavi; Fiscal Year: 2012
    ..The 22a subunit of LTCCs is responsible for trafficking of the protein to the plasma membrane and sarcolemma. The B2a subunit has been effectively upregulated in a rat myocytes using an adenovirus construct...
  10. Phospholemman and Na-Pump Function in Cardiac Myocytes
    Donald M Bers; Fiscal Year: 2013
    ..possibility that PLB (at least PLB human mutants that are relevant to human disease) can be misdirected to the sarcolemma and regulate NKA...
  11. Metabolic Regulation of Sodium Channels
    Samuel C Dudley; Fiscal Year: 2013
    ..on the Na+ channel, either by direct action on the channel complex or by causing channel isolation from the sarcolemma. Insight into Na+ channel regulation may allow mitigation of arrhythmic risk associated with low INa states...
  12. Structure-Function Analysis of Sarcospan
    RACHELLE HOPE CROSBIE-WATSON; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): In skeletal muscle, the dystrophin-glycoprotein complex is located at the sarcolemma and is composed of peripheral and integral membrane proteins...
  13. Endothelin Receptors and Progression to Heart Failure
    Francis G Spinale; Fiscal Year: 2011
    ..Since MT1-MMP possesses multiple biological actions and is robustly expressed on the myocyte sarcolemma, then it follows that MT1-MMP may play an important role in I/R injury...
  14. Coupled Gating of L-type Calcium Channels in Heart
    Luis F Santana; Fiscal Year: 2013
    ..2 channel activity varies along the sarcolemma of ventricular myocytes due to signaling micro-domains created by a subpopulation of these channels interacting ..
  15. Regulation of Syntrophin Function
    Stanley C Froehner; Fiscal Year: 2013
    ..transgenic results demonstrate that the PH1 domain is important in the association of nNOS with the DPC and the sarcolemma. We will examine the role of phosphatidylinositol lipids and PH1 binding proteins in this regulatory mechanism...
  16. Identification of genes responsible for sarcolemmal integrity in C. elegans
    Hongkyun Kim; Fiscal Year: 2013
    ..elegans for identifying molecular and cellular components that mediate the repair of damaged sarcolemma (muscle plasma membrane). By taking advantage of the transparency of the C...
  17. Mechanism and function of a novel purinergic signaling cascade in skeletal muscle
    ANDREW ALVIN VOSS; Fiscal Year: 2013
    ..the effects of chloride channel inhibition by P2Y1 receptors on the propagation of action potentials in the sarcolemma and transverse tubular system...
  18. Quantitative analysis of cAMP compartmentation in heart
    Jeffrey J Saucerman; Fiscal Year: 2013
    ..degradation, physical barriers, and buffering, which together help mediate selective PKA activity in cytosol, sarcolemma, caveolae, and nucleus. We will test this hypothesis through 3 Specific Aims...
  19. Costamere Defects in Muscular Dystrophies
    James M Ervasti; Fiscal Year: 2013
    ..protein assemblies in striated muscle cells that physically couple force-generating sarcomeres with the sarcolemma. Costameres are clearly important for normal muscle function because several constituent proteins are the ..
  20. IONIC CONTROL OF CARDIAC MUSCLE CONTRACTION: EC COUPLING
    Donald M Bers; Fiscal Year: 2013
    ..g. nucleus and sarcolemma) which influence systems from ion channels to transcriptional conntrol...
  21. Stretch Dependent Signaling in Dystrophic Skeletal Muscle
    CHRISTOPHER WILLIAM WARD; Fiscal Year: 2013
    ..X-ROS activates sarcolemmal stretch sensitive Ca2+ channels to elicit excessive Ca2+ influx across the sarcolemma. Furthermore, we show that the activation of X-ROS in the mdx is dependent on a significant microtubule ..
  22. Understanding the mechanism and role of cell membrane repair in Miyoshi Myopathy
    Jyoti K Jaiswal; Fiscal Year: 2012
    ..dystrophy (LGMD) 2B, caused by defects in dysferlin expression is associated with poor healing of wounded sarcolemma. Poor healing of wounded muscles is believed to be due to poor calcium-triggered vesicle exocytosis...
  23. CARDIAC & VASCULAR TOXICITY OF COCAINE
    James Morgan; Fiscal Year: 1991
    ..cardiovascular effects and toxicity of cocaine are due to its direct effects on muscle, including actions on the sarcolemma (i.e., local anesthetic, calcium channel blockade, effects on Na+/Ca++ exchange), sarcoplasmic reticulum (i.e...
  24. OXYGEN RADICALS, OXIDANTS AND MEMBRANE DYSFUNCTION
    RAKESH KUKREJA; Fiscal Year: 1990
    ..g., adriamycin). We have hypothesized that the major target organelles injured by free radical attack are the sarcolemma and sarcoplasmic reticulum which are the key regulators of myocardial contractility...
  25. ALTERED MEMBRANE PHOSPHOLIPIDS AND CARDIAC FUNCTION
    DIANE REIBEL; Fiscal Year: 1990
    ..Specifically, mitochondria, sarcoplasmic reticulum (SR) and sarcolemma (SL) will be isolated from hearts of rats fed varying amounts of menhaden oil (MO), a fish oil rich in n-3 ..
  26. Intermediate Filaments & Costamere Structure & Function
    Robert J Bloch; Fiscal Year: 2010
    Costameres are structures at the surface of striated muscle that align the sarcolemma regularly with nearby myofibrils and transmit contractile force laterally, through the membrane to the extracellular matrix...
  27. The Sarcolemma in FSHD and in the myd Mouse
    Robert Bloch; Fiscal Year: 2003
    ..that, like other human dystrophies, such as Duchennes, Beckers, and some limb girdle muscular dystrophies, the sarcolemma of FHSD muscle is altered in ways that lead to muscle weakness and ultimately to muscle degeneration...
  28. CALCIUM REGULATION IN ARTERIAL MYOCYTES
    William Goldman; Fiscal Year: 1993
    ..The emphasis is on the relative roles of the SR and the sarcolemma in regulating the cellular response during agonist-induced activation...
  29. Role of Calpains in Plasma Membrane Repair
    RONALD MELLGREN; Fiscal Year: 2009
    ..studies have the potential to result in derivation of the first pharmacologic agent specifically designed to aid sarcolemma repair...
  30. ION CHANNEL TARGETS FOR CARDIAC GLYCOSIDE ACTIONS
    J Wasserstrom; Fiscal Year: 2000
    ..Other compelling evidence, however, suggests additional cellular actions, including direct actions on the sarcolemma and sarcoplasmic reticulum (SR), that contribute to their positive inotropic and/or toxic actions...
  31. CULTURED CARDIAC MYOCYTES--A MODEL OF IRON OVERLOAD
    Chaim Hershko; Fiscal Year: 1991
    ..These studies will proceed along 3 lines: I. Structural changes in lipid membranes isolated from the sarcolemma, mitochondria and lysosomes will be characterized by measuring their phospholipid, lysophospholipid, ..
  32. Inward Rectifier Potassium Channels and Cardiac Excitability
    Anatoli N Lopatin; Fiscal Year: 2010
    ..properties of lK1 are strongly dependent on membrane-specific expression of distinct Kir2 subunits in the outer sarcolemma, t-tubules or intercalated disks...
  33. PLASMALOGEN CATABOLISM DURNIG MYOCARDIAL ISCHEMIA
    David Ford; Fiscal Year: 1993
    ..by accelerated sarcolemmal phospholipid hydrolysis and plasmalogens are the major phospholipid constituents of sarcolemma, alterations in plasmalogen catabolism during myocardial ischemia have been virtually ignored...
  34. G PROTEIN-MEDIATED K+ CHANNEL ACTIVATION IN HEART
    GERDA BREITWIESER; Fiscal Year: 1993
    ..G protein turnover, and produce persistent activation), and second, with agents that perturb the fluidity of the sarcolemma. Another issue which will be addressed is the lack of potassium channel activation in the absence of ACh, ..
  35. DIASTOLIC PROPERTIES OF DEVELOPING MYOCARDIUM
    Kent Thornburg; Fiscal Year: 1993
    ..on the diastolic properties of the mature fetal left ventricle; 4) to determine the relative importance of sarcolemma vs...
  36. ACCELERATED PLASMALOGEN CATABOLISM DURING ISCHEMIA
    David Ford; Fiscal Year: 1999
    Since plasmalogens are the predominant phospholipid constituents of sarcolemma and are selectively hydrolyzed during myocardial ischemia, the goal of the proposed studies is the demonstration that accelerated plasmalogen catabolism is a ..
  37. Exercise Training and Myocardial K-ATP Channel Function
    RUSSELL MOORE; Fiscal Year: 2007
    ..In the heart, ATP-sensitive K channels exist on the sarcolemma (sI-KATP) and in mitochondria (mito-KATP) and both have been implicated in protecting the heart against ischemic ..
  38. The role of electroporation in defibrillation
    Igor Efimov; Fiscal Year: 2007
    ..On the other hand, electroporation is frequently a method of choice for delivery of large molecules across sarcolemma, a key in the success of future molecular medicine...
  39. REGULATION OF THE CARDIAC CALCIUM CHANNEL
    James Marsh; Fiscal Year: 1993
    ..and paracrine factors determine how many functional copies of the L-type calcium channel are present in the sarcolemma of heart cells...
  40. MYOCARDIAL FA METABOLISM GENE-GENE INTERACTIONS IN HYPERTENSIVE HEART DISEASE
    Lisa De Las Fuentes; Fiscal Year: 2010
    ..Although some LCFAs diffuse passively across the sarcolemma, a protein-facilitated transporter, the fatty acid translocase (FAT or CD36) confers tissue-specific expression ..
  41. PLASMALOGEN-SELECTIVE PLA2 ACTIVATION IN ISCHEMIA
    Jane McHowat; Fiscal Year: 1999
    ..accompanied by accelerated phospholipid catabolism resulting in the accumulation of lysophospholipids in the sarcolemma which directly contributes to arrhythmogenesis. Dr...
  42. SODIUM/CALCIUM EXCHANGE ACROSS CARDIAC SARCOLEMMA
    GEORGE LINDENMAYER; Fiscal Year: 1993
    ....
  43. REGULATION OF MYOCARDIAL SYNTHESIS, RELEASE & PROCESSING
    James Greenwald; Fiscal Year: 1993
    ..The central hypothesis of the proposed research is that mechanical perturbation (i.e. stretch) of the myocyte sarcolemma and other atriopeptin secretagogues activate phospholipase C, which results in the formation of two putative ..
  44. Cytoskeletal Basis of Ventricular Arrhythmias
    Matteo Vatta; Fiscal Year: 2005
    ..Cytoskeletal proteins such as dystrophin, the major link between the sarcomere and the sarcolemma in cardiac cells, have been involved in sudden cardiac death...
  45. Molecular Physiology of Neuromusclar Diseases
    Stephen Cannon; Fiscal Year: 2007
    ..of skeletal muscle in which mutations of voltage-gated ion channels alter the electrical excitability of the sarcolemma. The long-term goals of this project are to characterize the functional defects of mutant channels and to ..
  46. MEMBRANE-BOUND ENZYMES AND DRUG RECEPTORS IN MUSCLE
    Thomas Mittag; Fiscal Year: 1980
    A study of the properties of membrane-bound receptors and enzymes in skeletal muscle sarcolemma in normal, denervated and dystrophic states will be undertaken...
  47. CHARACTERIZATION OF NA-CA EXCHANGE--IMMATURE MYOCARDIUM
    Michael Artman; Fiscal Year: 1990
    ..However, relatively little is known of the calcium regulatory properties of the sarcolemma with regard to perinatal development...
  48. QUANTITATIVE MORPHOLOGY OF CARDIAC HYPERTROPHY
    Thomas Marino; Fiscal Year: 1992
    ..in the connective tissue components of the ventricular myocardium and a change in myocyte size and external sarcolemma surface area/ cell volume ratio...
  49. CARDIAC SARCOLEMMAL ION TRANSPORT AND THE 15-KDA PROTEIN
    Richard Kovacs; Fiscal Year: 1990
    The cardiac plasma membrane or sarcolemma (SL) regulates ion flux into and out of the cardiac cell through a series of channels, pumps and antiporters...
  50. MECHANISMS OF EXERCISE INDUCED MUSCLE FIBER INJURY
    Richard Steinhardt; Fiscal Year: 2000
    The goal of this research is to understand the mechanisms by which transient tears at the sarcolemma during exercise might lead to long-term damage within a muscle cell...
  51. Local Signals and Macromolecular Architecture in Heart
    Terry Rogers; Fiscal Year: 2006
    ..of cardiac myocytes, particularly the spectrin and dystrophin networks that organize and stabilize the sarcolemma and t-tubular membranes...
  52. CONTROL OF 02 TRANSPORT IN HEART AND SKELETAL MUSCLES
    THOMAS GAYESKI; Fiscal Year: 1992
    ..hemoglobin (Hb) saturation in individual red cells, and Mb saturation in the pericapillary region close to the sarcolemma. The delta PO2 between Hb and pericapillary myoplasm defines the driving force for O2 release from blood...
  53. Plasmalogen Metabolism by iPLA2 in Ischemic Myocardium
    Jane McHowat; Fiscal Year: 2005
    ..If these metabolites gain access to the ventricular myocyte sarcolemma, they may also contribute to arrhythmogenesis...
  54. MOLECULAR BIOLOGY OF CARDIAC SODIUM/CALCIUM EXCHANGE
    KENNETH PHILIPSON; Fiscal Year: 2001
    The sarcolemma (plasma membrane) of myocardial cells has high levels of Na+-Ca2+ exchange activity. Na+-Ca 2+ exchange is an important mechanism for regulating myocardial Ca 2+ and thereby also regulates contractility...
  55. Epsilon-sarcoglycan & pathogenesis of muscular dystrophy
    YVONNE KOBAYASHI; Fiscal Year: 2003
    ..the function of epsilon-sarcoglycan within the epsilon-beta-gamma-delta sarcoglycan complex, as well as at the sarcolemma, and how its function works together with the functional role of the dystrophin-glycoprotein complex in the ..
  56. QUANTITATIVE LOCALIZATION OF ELECTROLYTES IN HEART
    JOHN TORMEY; Fiscal Year: 1991
    ..1) Two Ca pools at cell surface may be related to contractility, namely, the sarcolemma and the interstitium...
  57. MOLECULAR STUDY OF THE MYOCARDIAL C1/HCO3 EXCHANGER
    PANAGIOTIS PAPAGEORGIOU; Fiscal Year: 1999
    ..homeostasis, but recent evidence has suggested that chloride and bicarbonate movements across the cardiac sarcolemma may affect arrhythmogenesis...
  58. SINGLE SKETETAL MUSCLE FIBER IMPAIRMENT WITH AGING
    Osvaldo Delbono; Fiscal Year: 2000
    ..The molecule that transduces sarcolemma voltage into changes in myoplasmic calcium concentrations is the dihydropyridine receptor (DHPR)...
  59. CALCIUM AND THE PATHOPHYSIOLOGY OF THE ADULT HEART CELL
    ROBERT HAWORTH; Fiscal Year: 2001
    ..While Ca influx through the sarcolemma opens SR Ca release channels in the cleft, it is not yet even known if this is the major release pathway for SR ..
  60. SARCOLEMMAL ORGANIZATION OF EXTRACULAR MUSCLE
    John Porter; Fiscal Year: 2004
    ..We currently have almost no knowledge of the cell/molecular substrate for stabilizing the EOM membrane, or sarcolemma, and for formation and maintenance of specializations at the neuromuscular function...
  61. INTERACTION OF IONS WITH CARDIAC SARCOLEMMA
    KENNETH PHILIPSON; Fiscal Year: 1992
    ..It is speculated that the cells will adjust rates of synthesis and degradation of different ion transporters in response to interventions...
  62. SKELETAL MUSCLE IMPAIRMENT IN AGING
    Osvaldo Delbono; Fiscal Year: 2000
    ..The molecule that transduces sarcolemma voltage into changes in myoplasmic calcium concentrations is the dihydropyridine receptor (DHPR)...
  63. REGULATION OF MYOTENDINOUS JUNCTION STRUCTURE
    James Tidball; Fiscal Year: 2001
    ..Hind limb immobilization at shortened length followed by remobilization will be used to examine the effect of inhibition of NOS on sarcomere addition. ..
  64. IN VITRO MODEL OF PRECONDITIONING WITH ISOLATED MYOCYTES
    CHARLES GANOTE; Fiscal Year: 2001
    ....
  65. Function and regulation of cardiac Na transporters
    Donald Hilgemann; Fiscal Year: 2008
    ..4) In parallel with the experimental program, we will develop improved mathematical models of cardiac ion homeostasis with an emphasis on these three Na transporters. ..