hl 60 cells

Summary

Summary: A promyelocytic cell line derived from a patient with ACUTE PROMYELOCYTIC LEUKEMIA. HL-60 cells lack specific markers for LYMPHOID CELLS but express surface receptors for FC FRAGMENTS and COMPLEMENT SYSTEM PROTEINS. They also exhibit phagocytic activity and responsiveness to chemotactic stimuli. (From Hay et al., American Type Culture Collection, 7th ed, pp127-8)

Top Publications

  1. ncbi Prevention of apoptosis by Bcl-2: release of cytochrome c from mitochondria blocked
    J Yang
    Department of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322, USA
    Science 275:1129-32. 1997
  2. ncbi Superoxide dismutase as a target for the selective killing of cancer cells
    P Huang
    Department of Experimental Therapeutics, The University of Texas M D Anderson Cancer Center, Houston 77030, USA
    Nature 407:390-5. 2000
  3. ncbi Cell fates as high-dimensional attractor states of a complex gene regulatory network
    Sui Huang
    Vascular Biology Program, Departments of Pathology and Surgery, Children s Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA
    Phys Rev Lett 94:128701. 2005
  4. pmc Rac and Cdc42 play distinct roles in regulating PI(3,4,5)P3 and polarity during neutrophil chemotaxis
    Supriya Srinivasan
    Department of Cellular and Molecular Pharmacology and the Cardiovascular Research Institute, University of California, San Francisco, CA 94143, USA
    J Cell Biol 160:375-85. 2003
  5. ncbi Anaplasma phagocytophilum AnkA secreted by type IV secretion system is tyrosine phosphorylated by Abl-1 to facilitate infection
    Mingqun Lin
    Department of Veterinary Biosciences, The Ohio State University, 1925 Coffey Road, Columbus, OH 43210, USA
    Cell Microbiol 9:2644-57. 2007
  6. pmc A PtdInsP(3)- and Rho GTPase-mediated positive feedback loop regulates neutrophil polarity
    Orion D Weiner
    Department of Cell Biology, Harvard Medical School and Beth Israel Deaconess Medical Center, 240 Longwood Ave C 1, Boston, MA 02115, USA
    Nat Cell Biol 4:509-13. 2002
  7. ncbi Thymoquinone induces apoptosis through activation of caspase-8 and mitochondrial events in p53-null myeloblastic leukemia HL-60 cells
    Mohamed A El-Mahdy
    Department of Radiology, Ohio State University, Columbus, OH 43210, USA
    Int J Cancer 117:409-17. 2005
  8. ncbi Functional characterization of mitochondria in neutrophils: a role restricted to apoptosis
    N A Maianski
    Emma Childrens Hospital, Academic Medical Center, University of Amsterdam, The Netherlands
    Cell Death Differ 11:143-53. 2004
  9. ncbi The antitumor ether lipid ET-18-OCH(3) induces apoptosis through translocation and capping of Fas/CD95 into membrane rafts in human leukemic cells
    C Gajate
    , , CSIC-Universidad de Salamanca, Campus Miguel de Unamuno, Salamanca, Spain
    Blood 98:3860-3. 2001
  10. ncbi Deimination of arginine residues in nucleophosmin/B23 and histones in HL-60 granulocytes
    Teruki Hagiwara
    Graduate School of Integrated Science, Yokohama City University, 22 2, Seto, Kanazawa Ku, Yokohama, 236 0027, Japan
    Biochem Biophys Res Commun 290:979-83. 2002

Detail Information

Publications288 found, 100 shown here

  1. ncbi Prevention of apoptosis by Bcl-2: release of cytochrome c from mitochondria blocked
    J Yang
    Department of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322, USA
    Science 275:1129-32. 1997
    ..Overexpression of Bcl-2 prevented the efflux of cytochrome c from the mitochondria and the initiation of apoptosis. Thus, one possible role of Bcl-2 in prevention of apoptosis is to block cytochrome c release from mitochondria...
  2. ncbi Superoxide dismutase as a target for the selective killing of cancer cells
    P Huang
    Department of Experimental Therapeutics, The University of Texas M D Anderson Cancer Center, Houston 77030, USA
    Nature 407:390-5. 2000
    ..Our results indicate that targeting SOD may be a promising approach to the selective killing of cancer cells, and that mechanism-based combinations of SOD inhibitors with free-radical-producing agents may have clinical applications...
  3. ncbi Cell fates as high-dimensional attractor states of a complex gene regulatory network
    Sui Huang
    Vascular Biology Program, Departments of Pathology and Surgery, Children s Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA
    Phys Rev Lett 94:128701. 2005
    ....
  4. pmc Rac and Cdc42 play distinct roles in regulating PI(3,4,5)P3 and polarity during neutrophil chemotaxis
    Supriya Srinivasan
    Department of Cellular and Molecular Pharmacology and the Cardiovascular Research Institute, University of California, San Francisco, CA 94143, USA
    J Cell Biol 160:375-85. 2003
    ..We conclude that Rac plays a dominant role in the PI(3,4,5)P3-dependent positive feedback loop required for forming a leading edge, whereas location and stability of the leading edge are regulated by Cdc42...
  5. ncbi Anaplasma phagocytophilum AnkA secreted by type IV secretion system is tyrosine phosphorylated by Abl-1 to facilitate infection
    Mingqun Lin
    Department of Veterinary Biosciences, The Ohio State University, 1925 Coffey Road, Columbus, OH 43210, USA
    Cell Microbiol 9:2644-57. 2007
    ....
  6. pmc A PtdInsP(3)- and Rho GTPase-mediated positive feedback loop regulates neutrophil polarity
    Orion D Weiner
    Department of Cell Biology, Harvard Medical School and Beth Israel Deaconess Medical Center, 240 Longwood Ave C 1, Boston, MA 02115, USA
    Nat Cell Biol 4:509-13. 2002
    ..Emergent properties of positive and negative regulatory links between PtdInsP(3) and Rho family GTPases may constitute a broadly conserved module for the establishment of cell polarity during eukaryotic chemotaxis...
  7. ncbi Thymoquinone induces apoptosis through activation of caspase-8 and mitochondrial events in p53-null myeloblastic leukemia HL-60 cells
    Mohamed A El-Mahdy
    Department of Radiology, Ohio State University, Columbus, OH 43210, USA
    Int J Cancer 117:409-17. 2005
    ..Activated caspase-8 initiates the release of cytochrome c during TQ-induced apoptosis. Overall, these results offer a potential mechanism for TQ-induced apoptosis in p53-null HL-60 cancer cells...
  8. ncbi Functional characterization of mitochondria in neutrophils: a role restricted to apoptosis
    N A Maianski
    Emma Childrens Hospital, Academic Medical Center, University of Amsterdam, The Netherlands
    Cell Death Differ 11:143-53. 2004
    ..We suggest that other proapoptotic factors such as Smac/DIABLO and HtrA2/Omi, which are massively released from the mitochondria, have an important role in neutrophil apoptosis...
  9. ncbi The antitumor ether lipid ET-18-OCH(3) induces apoptosis through translocation and capping of Fas/CD95 into membrane rafts in human leukemic cells
    C Gajate
    , , CSIC-Universidad de Salamanca, Campus Miguel de Unamuno, Salamanca, Spain
    Blood 98:3860-3. 2001
    ..This translocation of Fas into membrane rafts may provide a mechanism for amplifying Fas signaling by reorganization of membrane microdomains...
  10. ncbi Deimination of arginine residues in nucleophosmin/B23 and histones in HL-60 granulocytes
    Teruki Hagiwara
    Graduate School of Integrated Science, Yokohama City University, 22 2, Seto, Kanazawa Ku, Yokohama, 236 0027, Japan
    Biochem Biophys Res Commun 290:979-83. 2002
    ..The citrulline content of histones amounted to about 10% of the histone molecules. We discuss the implications of deimination of these proteins for their nuclear functions...
  11. ncbi Human PAD4 regulates histone arginine methylation levels via demethylimination
    Yanming Wang
    Department of Genetic Medicine, Weill Medical College of Cornell University, 1300 York Avenue, New York, NY 10021, USA
    Science 306:279-83. 2004
    ..Moreover, PAD4 activity is linked with the transcriptional regulation of estrogen-responsive genes in MCF-7 cells. These data suggest that PAD4 mediates gene expression by regulating Arg methylation and citrullination in histones...
  12. ncbi Arsenic trioxide selectively induces acute promyelocytic leukemia cell apoptosis via a hydrogen peroxide-dependent pathway
    Y Jing
    Rochelle Belfer Chemotherapy Foundation Laboratory, Division of Neoplastic Diseases, Department of Medicine, Mount Sinai Medical Center, New York, NY, USA
    Blood 94:2102-11. 1999
    ....
  13. ncbi Resveratrol arrests the cell division cycle at S/G2 phase transition
    F D Ragione
    Medical School, Second University of Naples, Naples, Italy
    Biochem Biophys Res Commun 250:53-8. 1998
    ..These data demonstrate that resveratrol causes a complete and reversible cell cycle arrest at the S phase checkpoint...
  14. pmc Role of NADPH oxidase in arsenic-induced reactive oxygen species formation and cytotoxicity in myeloid leukemia cells
    Wen Chien Chou
    Program of Human Genetics and Molecular Biology, School of Medicine, Johns Hopkins University, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 101:4578-83. 2004
    ..Our findings pinpoint the arsenic target of ROS production and provide a conceptual basis for an anticancer regimen...
  15. ncbi Activation of extracellular signal-regulated kinases (ERKs) defines the first phase of 1,25-dihydroxyvitamin D3-induced differentiation of HL60 cells
    X Wang
    Department of Pathology and Laboratory Medicine, UMDNJ-New Jersey Medical School, 185 South Orange Avenue, Newark, New Jersey 07103, USA
    J Cell Biochem 80:471-82. 2001
    ....
  16. ncbi A role for oxidative stress in apoptosis: oxidation and externalization of phosphatidylserine is required for macrophage clearance of cells undergoing Fas-mediated apoptosis
    Valerian E Kagan
    Department of Environmental and Occupational Health, University of Pittsburgh, Pittsburgh, PA 15260, USA
    J Immunol 169:487-99. 2002
    ..Taken together, our data suggest that the presence of PS-OX in conjunction with nonoxidized PS on the cell surface is an important signal for macrophage clearance of apoptotic cells...
  17. ncbi Expression of the miR-17-92 polycistron in chronic myeloid leukemia (CML) CD34+ cells
    Letizia Venturini
    Department of Hematology, Hemostasis, and Oncology, Hannover Medical School, Germany
    Blood 109:4399-405. 2007
    ..Altered miRNA expression may contribute to the pathophysiology of the disease and may provide potential targets for therapeutic intervention...
  18. ncbi The Vav binding site of the non-receptor tyrosine kinase Syk at Tyr 348 is critical for beta2 integrin (CD11/CD18)-mediated neutrophil migration
    Jurgen Schymeinsky
    Department of Physiology, Ludwig Maximilians Universitat Munchen, Schillerstr 44, D 80336 MUnchen, Germany
    Blood 108:3919-27. 2006
    ..These findings indicate that Syk is critical for beta(2) integrin-mediated neutrophil migration in vitro and plays a fundamental role in neutrophil recruitment during the inflammatory response in vivo...
  19. ncbi Effect of cytarabine and decitabine in combination in human leukemic cell lines
    Taichun Qin
    Department of Leukemia, The University of Texas M D Anderson Cancer Center, Houston, Texas 77030, USA
    Clin Cancer Res 13:4225-32. 2007
    ..5-Aza-2'-deoxycytidine (DAC) is a cytosine analogue that inhibits DNA methylation and also has activity in myeloid leukemia. Therefore, we investigated combining these two drugs in human leukemia cell lines in vitro...
  20. ncbi The human EGF-TM7 receptor EMR3 is a marker for mature granulocytes
    Mourad Matmati
    Department of Experimental Immunology, Academic Medical Center, University of Amsterdam, 1100 DE Amsterdam, The Netherlands
    J Leukoc Biol 81:440-8. 2007
    ..These results demonstrate that the expression of EGF-TM7 receptors on myeloid cells is differentially regulated. EMR3 is the first family member found mainly on granulocytes...
  21. ncbi Antiapoptotic action of focal adhesion kinase (FAK) against ionizing radiation
    Tadashi Kasahara
    Department of Biochemistry, Kyoritsu College of Pharmacy, Shibakoen 1 5 30, Minato ku, Tokyo, 105 8512, Japan
    Antioxid Redox Signal 4:491-9. 2002
    ..These results indicated that mitochondria pathway is required for IR-induced apoptosis, and FAK overexpression prevents this pathway, thus rendering antiapoptotic states...
  22. ncbi Loss of phospholipid asymmetry and surface exposure of phosphatidylserine is required for phagocytosis of apoptotic cells by macrophages and fibroblasts
    V A Fadok
    Program in Cell Biology, Department of Pediatrics, National Jewish Medical and Research Center, Denver, Colorado 80206, USA
    J Biol Chem 276:1071-7. 2001
    ..These observations directly demonstrate that loss of phospholipid asymmetry and PS expression is required for phagocyte engulfment of apoptotic cells and imply a critical, if not obligatory, role for PS recognition in the uptake process...
  23. ncbi Down-regulation of the mitochondrial translation system during terminal differentiation of HL-60 cells by 12-O-tetradecanoyl-1-phorbol-13-acetate: comparison with the cytoplasmic translation system
    Nono Takeuchi
    Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Building FSB 401, 5 1 5, Kashiwanoha, Kashiwa, Chiba Prefecture 277 8562, Japan
    J Biol Chem 278:45318-24. 2003
    ....
  24. pmc Phosphatidylinositol transfer protein beta displays minimal sphingomyelin transfer activity and is not required for biosynthesis and trafficking of sphingomyelin
    Bruno Segui
    Department of Physiology, University College London, London WC1E 6JJ, U K
    Biochem J 366:23-34. 2002
    ..Altogether, these observations do not support the suggestion that PITPbeta participates in the transfer of SM, the regulation of SM biosynthesis or its intracellular trafficking...
  25. ncbi Anaplasma phagocytophilum delays spontaneous human neutrophil apoptosis by modulation of multiple apoptotic pathways
    Yan Ge
    Department of Veterinary Biosciences, The Ohio State University, Columbus, 43210, USA
    Cell Microbiol 8:1406-16. 2006
    ..These data point to a novel mechanism induced by A. phagocytophilum involving both extrinsic and intrinsic pathways to ensure to delay the apoptosis of host neutrophils...
  26. ncbi Divergent signals and cytoskeletal assemblies regulate self-organizing polarity in neutrophils
    Jingsong Xu
    Department of Cellular and Molecular Pharmacology and the Cardiovascular Research Institute, University of California, San Francisco, San Francisco, CA 94143, USA
    Cell 114:201-14. 2003
    ..The latter effect explains both the neutrophil's ability to polarize in uniform concentrations of chemoattractant and its response to reversal of an attractant gradient by performing a U-turn...
  27. ncbi Enhancement of antineoplastic action of 5-aza-2'-deoxycytidine by phenylbutyrate on L1210 leukemic cells
    Maryse Lemaire
    Department de Pharmacologie, Universite de Montreal, Centre de Recherche Pediatrique, Hopital Sainte Justine, 3175 Cote Sainte Catherine, Montreal, Quebec H3T 1C5, Canada
    Leuk Lymphoma 45:147-54. 2004
    ..We also observed an enhancement of the antineoplastic activity of this combination in mice with L1210 leukemia. These data provide a rationale to investigate 5-AZA-CdR and PB in patients with advanced leukemia...
  28. ncbi Promyelocytic HL60 cells express NADPH oxidase and are excellent targets in a rapid spectrophotometric microplate assay for extracellular superoxide
    Olga Teufelhofer
    Institute of Cancer Research, Division Oncological Toxicology, University of Vienna, Austria
    Toxicol Sci 76:376-83. 2003
    ....
  29. ncbi Microtubule damaging agents induce apoptosis in HL 60 cells and G2/M cell cycle arrest in HT 29 cells
    Chia Jen Tseng
    Graduate Institute of Biomedical Technology, Taipei Medical University, No 250, Wu Hsing Street, Taipei 110, Taiwan, ROC
    Toxicology 175:123-42. 2002
    ..increased leakage of cytochrome c from mitochondria into cytosolic fraction were detected in the PDP-treated HL 60 cells. These listed intracellular events were interpreted to lead to the apoptosis observed in PDP-treated HL 60 ..
  30. ncbi Acoustic activation of drug delivery from polymeric micelles: effect of pulsed ultrasound
    A Marin
    Department of Bioengineering, University of Utah, Salt Lake City, UT 84112, USA
    J Control Release 71:239-49. 2001
    ..The second mechanism is based on the perturbation of cell membranes that results in the increased uptake of the micellar-encapsulated drug. The intracellular uptake of Pluronic micelles was confirmed by fluorescence microscopy...
  31. pmc Arginine methylation provides epigenetic transcription memory for retinoid-induced differentiation in myeloid cells
    Balint L Balint
    Department of Biochemistry and Molecular Biology, Research Center for Molecular Medicine, University of Debrecen, Medical and Health Science Center, Nagyerdei krt 98, Debrecen H 4012, Hungary
    Mol Cell Biol 25:5648-63. 2005
    ....
  32. ncbi Neutrophil maturation and the role of retinoic acid
    N D Lawson
    Department of Biology, Yale University School of Medicine, New Haven, CT 06510, USA
    Exp Hematol 27:1355-67. 1999
    ..Here we present a review of the mechanism of retinoic acid receptor action and the major evidence showing that normal retinoid signaling is required for neutrophil maturation...
  33. ncbi Translational control of specific genes during differentiation of HL-60 cells
    A M Krichevsky
    Department of Molecular Virology, The Faculty of Medicine, Hebrew University of Jerusalem, P O Box 12272, Jerusalem 91120, Israel
    J Biol Chem 274:14295-305. 1999
    ....
  34. ncbi c-MYC interacts with INI1/hSNF5 and requires the SWI/SNF complex for transactivation function
    S W Cheng
    Department of Molecular Genetics, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Nat Genet 22:102-5. 1999
    ..Our results suggest that the SWI/SNF complex is necessary for c-MYC-mediated transactivation and that the c-MYC-INI1 interaction helps recruit the complex...
  35. pmc Neutrophil microtubules suppress polarity and enhance directional migration
    Jingsong Xu
    Department of Cellular and Molecular Pharmacology and Cardiovascular Research Institute, University of California, San Francisco, CA 94143 0450, USA
    Proc Natl Acad Sci U S A 102:6884-9. 2005
    ..Thus, microtubules normally balance backness vs. frontness signals, preventing backness from reducing the strength of pseudopods and from impairing directional migration...
  36. pmc Leukocyte adhesion to vascular endothelium induces E-selectin linkage to the actin cytoskeleton
    M Yoshida
    Department of Pathology, Brigham and Women s Hospital, Boston, MA 02115, USA
    J Cell Biol 133:445-55. 1996
    ....
  37. ncbi A hematopoietic cell L-selectin ligand that is distinct from PSGL-1 and displays N-glycan-dependent binding activity
    R Sackstein
    Bone Marrow Transplant Service, Department of Medicine, Massachusetts General Hospital, Boston, MA, USA
    Blood 96:2765-74. 2000
    ..This novel ligand may help mediate L-selectin-dependent cell-cell adhesive interactions within the cytoarchitecture of the bone marrow microenvironment. (Blood. 2000;96:2765-2774)..
  38. ncbi Thalidomide inhibits growth of tumors through COX-2 degradation independent of antiangiogenesis
    Gang jun Du
    Department of Pharmacology, Pharmacy College of He nan University, Kaifeng 475001, He Nan, China
    Vascul Pharmacol 43:112-9. 2005
    ..Effect of thalidomide on COX-1 and COX-2 in vivo was consistent with that of in vitro. These results demonstrated that thalidomide might inhibit growth of tumors through COX-2 degradation independent of antiangiogenesis...
  39. ncbi Differential gene expression in retinoic acid-induced differentiation of acute promyelocytic leukemia cells, NB4 and HL-60 cells
    Ki Hwan Lee
    Department of Biochemistry, College of Medicine, Hanyang University, Seoul, Republic of Korea
    Biochem Biophys Res Commun 296:1125-33. 2002
    ..Further studies will be needed to define the important molecular pathways involved in granulocytic differentiation by ATRA in APL cells...
  40. ncbi Regulation of NRAMP1 gene expression by 1alpha,25-dihydroxy-vitamin D(3) in HL-60 phagocytes
    E A Roig
    INRS Institut Armand Frappier, 531 Boulevard des Prairies, Laval, H7V 1B7 Quebec, Canada
    J Leukoc Biol 71:890-904. 2002
    ..Thus, VD genomic effects stimulate NRAMP1 transcription and protein expression in maturing phagocytes...
  41. ncbi Molecular mechanism of violacein-mediated human leukemia cell death
    Carmen Verissima Ferreira
    Departamento de Bioquimica, Universidade Estadual de Campinas, Sao Paulo, Brazil
    Blood 104:1459-64. 2004
    ..Hence, violacein represents the first member of a novel class of cytotoxic drugs mediating apoptosis of HL60 cells by way of the specific activation of TNF receptor 1...
  42. ncbi Hydroquinone-induced apoptosis in HL-60 cells
    Hiroshi Terasaka
    Department of Oral Diagnosis, Meikai University School of Dentistry, Saitama, Japan
    Anticancer Res 25:161-70. 2005
    ..The activation of caspase-3 by HQ/NAC combinations suggests that NAC, a precursor of intracellular glutathione synthesis, acts as a co-catalyst during HQ-induced apoptosis...
  43. ncbi DNA repair contributes to the drug-resistant phenotype of primary acute myeloid leukaemia cells with FLT3 internal tandem duplications and is reversed by the FLT3 inhibitor PKC412
    C H Seedhouse
    Department of Academic Haematology, School of Molecular Medical Sciences, Nottingham University Hospitals and University of Nottingham, Nottingham, UK
    Leukemia 20:2130-6. 2006
    ....
  44. ncbi Dihydroartemisinin induces apoptosis in HL-60 leukemia cells dependent of iron and p38 mitogen-activated protein kinase activation but independent of reactive oxygen species
    Jin Jian Lu
    Division of Anti tumor Pharmacology, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, P R China
    Cancer Biol Ther 7:1017-23. 2008
    ..Altogether, our data uncover that DHA induces apoptosis is dependent of iron and p38 MAPK activation but not ROS in HL-60 cells...
  45. ncbi The acute-phase protein alpha 1-acid glycoprotein (AGP) induces rises in cytosolic Ca2+ in neutrophil granulocytes via sialic acid binding immunoglobulin-like lectins (siglecs)
    Peter Gunnarsson
    Division of Pharmacology, Department of Medicine and Care, Linkoping University, S 581 85 Linkoping, Sweden
    FASEB J 21:4059-69. 2007
    ..Our report provides the first evidence for a signaling capacity by AGP through a defined receptor. Pre-engagement of L-selectin significantly enhanced this signaling capacity...
  46. ncbi Embelin derivatives and their anticancer activity through microtubule disassembly
    Minjuan Xu
    State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Xueyuan Road 38, 100083 Beijing, P R China
    Planta Med 71:944-8. 2005
    ..Treatment with 10 microM of 1 and 2 for 24 h induced apoptosis in HL-60 cells. This evidence suggests that both 1 and 2 are promising novel antimitotic and anticancer molecules targeting microtubular proteins...
  47. pmc Multistable and multistep dynamics in neutrophil differentiation
    Hannah H Chang
    Vascular Biology Program, Department of Pathology and Surgery, Children s Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA
    BMC Cell Biol 7:11. 2006
    ..However, the dynamics of mammalian cell differentiation has not been analyzed with respect to bistability...
  48. pmc Zosuquidar restores drug sensitivity in P-glycoprotein expressing acute myeloid leukemia (AML)
    Ruoping Tang
    INSERM, U872 Equipe 18 Paris, F 75006 France
    BMC Cancer 8:51. 2008
    ..In this paper, the capacity of zosuquidar, a specific P-gp modulator, to reverse chemoresistance was examined in both leukemia cell lines and primary AML blasts...
  49. ncbi Identification of Ki23819, a highly potent inhibitor of kinase activity of mutant FLT3 receptor tyrosine kinase
    Y Komeno
    Department of Hematology and Oncology, Graduate School of Medicine, University of Tokyo, Tokyo 113 8655, Japan
    Leukemia 19:930-5. 2005
    ..FLT3-ITD-dependent activation of the downstream signaling proteins ERK and STAT5 was also inhibited within similar concentration ranges. Thus, Ki23819 is a potent in vitro inhibitor of FLT3...
  50. ncbi Impact of cholesterol depletion on shape changes, actin reorganization, and signal transduction in neutrophil-like HL-60 cells
    Verena Niggli
    Department of Pathology, University of Bern, CH 3010 Bern, Switzerland
    Exp Cell Res 296:358-68. 2004
    ....
  51. ncbi Retinoic acid treated HL60 cells express CEACAM1 (CD66a) and phagocytose Neisseria gonorrhoeae
    Milica Pantelic
    Department of Microbiology and Immunology, Division of Infectious Diseases, Walther Oncology Center, Indiana University School of Medicine, Indianapolis, IN, USA
    FEMS Immunol Med Microbiol 42:261-6. 2004
    ..Our results indicate that the level of expression of CEACAM1 in HL60 cells can be regulated by treatment with RA in a differentiation-dependent manner, and that this is important for phagocytosis of OpaI-expressing gonococci or E. coli...
  52. ncbi Nuclear envelope and chromatin compositional differences comparing undifferentiated and retinoic acid- and phorbol ester-treated HL-60 cells
    A L Olins
    Foundation for Blood Research, P O Box 190, 69 U S Route One, Scarborough, Maine 04070 0190, USA
    Exp Cell Res 268:115-27. 2001
    ....
  53. ncbi Active extracts of wild fruiting bodies of Antrodia camphorata (EEAC) induce leukemia HL 60 cells apoptosis partially through histone hypoacetylation and synergistically promote anticancer effect of trichostatin A
    Mei Chin Lu
    Graduate Institute of Natural Products, School of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan
    Arch Toxicol 83:121-9. 2009
    ..We found that the ethanol extract from wild fruiting bodies of Antrodia camphorata (EEAC) could induce HL 60 cells apoptosis via histone hypoacetylation, up-regulation of histone deacetyltransferase 1 (HDAC 1), and down-..
  54. ncbi Insights into pathogen immune evasion mechanisms: Anaplasma phagocytophilum fails to induce an apoptosis differentiation program in human neutrophils
    Dori L Borjesson
    Department of Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, St Paul, MN 55108, USA
    J Immunol 174:6364-72. 2005
    ..phagocytophilum fails to trigger the apoptosis differentiation program usually induced by bacteria, and 2) a protein or molecule on the pathogen surface can mediate an early delay in spontaneous neutrophil apoptosis...
  55. ncbi Orderly pattern of development of the autoantibody response in (New Zealand White x BXSB)F1 lupus mice: characterization of target antigens and antigen spreading by two-dimensional gel electrophoresis and mass spectrometry
    Sandrine Thébault
    Institut de la Santé et de la Recherche Médicale Unité 519, Faculte de Medecine et de Pharmacie, Hopital Charles Nicolle, Rouen, France
    J Immunol 169:4046-53. 2002
    ....
  56. ncbi Molecular characterization of peptidylarginine deiminase in HL-60 cells induced by retinoic acid and 1alpha,25-dihydroxyvitamin D(3)
    K Nakashima
    Graduate School of Integrated Science, Yokohama City University, 22 2, Seto, Kanazawa Ku, Yokohama 236 0027, Japan
    J Biol Chem 274:27786-92. 1999
    ..Neither the enzyme activity nor the protein was found in macrophage-induced cells. These results suggested that expression of the PAD gene is tightly linked to myeloid differentiation...
  57. ncbi Nuclear localization of peptidylarginine deiminase V and histone deimination in granulocytes
    Katsuhiko Nakashima
    Graduate School of Integrated Science, Yokohama City University, 22 2 Seto, Kanazawa Ku, Yokohama 236 0027, Japan
    J Biol Chem 277:49562-8. 2002
    ..The major deiminated proteins were identified as histones H2A, H3, and H4. The implication of PAD V in histone modifications is discussed...
  58. ncbi N-hydroxy-N'-(3,4,5-trimethoxyphenyl)-3,4,5-trimethoxy-benzamidine, a novel resveratrol analog, inhibits ribonucleotide reductase in HL-60 human promyelocytic leukemia cells: synergistic antitumor activity with arabinofuranosylcytosine
    Philipp Saiko
    Clinical Institute of Medical and Chemical Laboratory Diagnostics, Medical University of Vienna, General Hospital of Vienna, Waehringer Guertel 18 20, A 1090 Vienna, Austria
    Int J Oncol 31:1261-6. 2007
    ..In addition, KITC exhibited synergistic combination effects when applied sequentially with Ara-C. Due to these promising results, KITC deserves further preclinical and in vivo testing...
  59. ncbi Cytotoxic and biochemical effects of 3,3',4,4',5,5'-hexahydroxystilbene, a novel resveratrol analog in HL-60 human promyelocytic leukemia cells
    Zsuzsanna Horvath
    Clinical Institute of Medical and Chemical Laboratory Diagnostics, General Hospital of Vienna, Medical University of Vienna, Vienna, Austria
    Exp Hematol 34:1377-84. 2006
    ..In the present study, the activity of a novel RV analog, 3,3',4,4',5,5'-hexahydroxystilbene (M8), was investigated in HL-60 human promyelocytic leukemia cells...
  60. ncbi Synergistic action of resveratrol, an ingredient of wine, with Ara-C and tiazofurin in HL-60 human promyelocytic leukemia cells
    Zsuzsanna Horvath
    Clinical Institute of Medical and Chemical Laboratory Diagnostics, General Hospital of Vienna, Medical University of Vienna, Vienna, Austria
    Exp Hematol 33:329-35. 2005
    ....
  61. ncbi Gallic acid inhibits ribonucleotide reductase and cyclooxygenases in human HL-60 promyelocytic leukemia cells
    Sibylle Madlener
    Clinical Institute of Medical and Chemical Laboratory Diagnostics, Medical University of Vienna, General Hospital of Vienna, Waehringer Guertel 18 20, A 1090 Vienna, Austria
    Cancer Lett 245:156-62. 2007
    ..Taken together, we identified novel biochemical effects of GA which could be the basis for further preclinical and in vivo studies...
  62. ncbi Comparison of the gene expression profiles of monocytic versus granulocytic lineages of HL-60 leukemia cell differentiation by DNA microarray analysis
    Ju Han Song
    College of Pharmacy and Research Institute of Drug Development, Chonnam National University, Kwangju, South Korea
    Life Sci 73:1705-19. 2003
    ..The identification of genes associated with a specific differentiation lineage may give important insights into functional and phenotypic differences between two lineages of HL-60 cell differentiation...
  63. ncbi Nanocytotoxicity: violacein and violacein-loaded poly (D, L-lactide-co-glycolide) nanoparticles acting on human leukemic cells
    Patricia S Melo
    Biochemistry Department, Instituto de Biologia, Universidade Estadual de Campinas UNICAMP, CP 6109, Campinas, SP, 13083 970, Brazil
    J Biomed Nanotechnol 5:192-201. 2009
    ..These methodologies are promising as diagnostic and mechanistic effects of nanoparticles in cell cultures...
  64. pmc To stabilize neutrophil polarity, PIP3 and Cdc42 augment RhoA activity at the back as well as signals at the front
    Alexandra Van Keymeulen
    Department of Cellular and Molecular Pharmacology, and Cardiovascular Research Institute, University of California, San Francisco, San Francisco, CA 94143, USA
    J Cell Biol 174:437-45. 2006
    ..Thus, PIP3 and Cdc42 maintain stable polarity with a single front and a single back not only by strengthening pseudopods but also, at longer range, by promoting RhoA-dependent actomyosin contraction at the trailing edge...
  65. ncbi Peribysins A-D, potent cell-adhesion inhibitors from a sea hare-derived culture of Periconia species
    Takeshi Yamada
    Osaka University of Pharmaceutical Sciences, 4 20 1, Nasahara, Takatsuki, Osaka 569 1094, Japan
    Org Biomol Chem 2:2131-5. 2004
    ..All these metabolites potently inhibited the adhesion of human-leukemia HL-60 cells to HUVEC. The activity of compound 4, exhibiting the most potent inhibitory activity, was 380 times as potent as herbimycin A (standard)...
  66. pmc Differential cell fates induced by all-trans retinoic acid-treated HL-60 human leukemia cells
    Munetaka Ozeki
    Division of Immunology, Beckman Research Institute of the City of Hope, 1500E Duarte Rd, Duarte, CA 91010, USA
    J Leukoc Biol 84:769-79. 2008
    ..These studies demonstrate distinct cell fates for ATRA-treated HL-60 cells that provide new insights into ATRA-induced cell differentiation...
  67. ncbi Optimization of nb-4 and hl-60 differentiation for use in opsonophagocytosis assays
    Roland A Fleck
    Division of Cell Biology and Imaging, National Institute for Biological Standards and Control, Blanche Lane, South Mimms, Potters Bar, Hertfordshire, United Kingdom EN6 3QG
    In Vitro Cell Dev Biol Anim 39:235-42. 2003
    ..Our results indicated that ATRA supplemented with vitamin D(3) and granulocyte colony-stimulating factor affords robust, rapid, and reproducible differentiation of both cell types...
  68. ncbi Analysis of Myc bound loci identified by CpG island arrays shows that Max is essential for Myc-dependent repression
    Daniel Y L Mao
    Ontario Cancer Institute Princess Margaret Hospital, University of Toronto, Ontario M5G 2M9, Canada
    Curr Biol 13:882-6. 2003
    ..Taken together, the identification and analysis of Myc bound target genes supports a model whereby Max plays an essential and universal role in the mechanism of Myc-dependent transcriptional regulation...
  69. ncbi Differential expression of VirB9 and VirB6 during the life cycle of Anaplasma phagocytophilum in human leucocytes is associated with differential binding and avoidance of lysosome pathway
    Hua Niu
    Department of Veterinary Biosciences, The Ohio State University, Columbus, OH 43210, USA
    Cell Microbiol 8:523-34. 2006
    ..phagocytophilum intracellular life cycle and suggest that bacterial developmental stages influence the nature of binding to the hosts and early avoidance of late endosome-lysosome pathway...
  70. pmc Neutrophil polarization: spatiotemporal dynamics of RhoA activity support a self-organizing mechanism
    Kit Wong
    Department of Cellular and Molecular Pharmacology, University of California, San Francisco, CA 94143 2140, USA
    Proc Natl Acad Sci U S A 103:3639-44. 2006
    ..Mutual incompatibility of frontness and backness is responsible for self-organization of neutrophil polarity...
  71. ncbi Cytotoxicity and apoptosis-inducing activity of bisphenol A and hydroquinone in HL-60 cells
    Hiroshi Terasaka
    Department of Oral Diagnosis, Meikai University School of Dentistry, Saitama, Japan
    Anticancer Res 25:2241-7. 2005
    ..The cytotoxicity of BPA was 2-fold less than that of HQ, whereas the apoptosis-inducing activity of BPA was 10-fold less than that of HQ...
  72. ncbi The interactions of Anaplasma phagocytophilum, endothelial cells, and human neutrophils
    Michael J Herron
    Department of Entomology, University of Minnesota, St Paul, Minnesota 55108, USA
    Ann N Y Acad Sci 1063:374-82. 2005
    ....
  73. ncbi Multidrug resistance protein attenuates gemtuzumab ozogamicin-induced cytotoxicity in acute myeloid leukemia cells
    Roland B Walter
    Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109 1024, USA
    Blood 102:1466-73. 2003
    ..Because MK-571 and CSA failed to affect cytotoxicity in a portion of Pgp-positive/MRP-positive AML samples, additional resistance mechanisms are likely important...
  74. ncbi Gene expression-based high-throughput screening(GE-HTS) and application to leukemia differentiation
    Kimberly Stegmaier
    Department of Pediatric Oncology, Dana Farber Cancer Institute and Children s Hospital Boston, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nat Genet 36:257-63. 2004
    ..The results indicate that GE-HTS may be a powerful, general approach for chemical screening...
  75. ncbi Chaetomugilins, new selectively cytotoxic metabolites, produced by a marine fish-derived Chaetomium species
    Muroga Yasuhide
    Osaka University of Pharmaceutical Sciences, Takatsuki, Osaka, Japan
    J Antibiot (Tokyo) 61:615-22. 2008
    ..These compounds exhibited significant growth inhibition against cultured P388 cells and HL-60 cells. In addition, chaetomugilins A, C and F showed selective cytotoxic activities against 39 human cancer cell lines...
  76. ncbi AML-1, PU.1, and Sp3 regulate expression of human bactericidal/permeability-increasing protein
    Andreas Lennartsson
    Department of Hematology, Lund University, Lund, Sweden
    Biochem Biophys Res Commun 311:853-63. 2003
    ..In conclusion, we provide evidence for AML-1, PU.1, and Sp3 cooperatively and directly mediating BPI-expression during myeloid differentiation...
  77. ncbi Targeted antitumoral dehydrocrotonin nanoparticles with L-ascorbic acid 6-stearate
    Lucas Frungillo
    Laboratório de Cultura de Células e Biofármacos, Instituto de Biologia, Universidade Estadual de Campinas, UNICAMP, Campinas, SP, Brazil
    J Pharm Sci 98:4796-807. 2009
    ..In vitro toxicity assays showed that NP-AAS-DHC suspension was more effective as an antitumoral than free DHC or NP-DHC and increased apoptosis induction by receptor-mediated pathway...
  78. pmc Differential expression of a C-terminal splice variant of phosphatidylinositol transfer protein beta lacking the constitutive-phosphorylated Ser262 that localizes to the Golgi compartment
    Clive P Morgan
    Lipid Signalling Group, Department of Physiology, University College London, 21 University Street, Rockefeller Building, London WC1E 6JJ, UK
    Biochem J 398:411-21. 2006
    ..The presence of PITPbeta sp variants adds an extra level of proteome complexity and, in rat liver, the single gene for PITPbeta gives rise to seven distinct protein species that can be resolved on the basis of their charge differences...
  79. ncbi Biological activities and pore formation of Clostridium perfringens beta toxin in HL 60 cells
    Masahiro Nagahama
    Department of Microbiology, Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Yamashiro cho, Tokushima 770 8514, Japan
    J Biol Chem 278:36934-41. 2003
    ..We conclude that the toxin acts on HL 60 cells by binding to lipid rafts and forming a functional oligomer with 228 kDa.
  80. ncbi Effects of extracts of Coriolus versicolor (I'm-Yunity) on cell-cycle progression and expression of interleukins-1 beta,-6, and -8 in promyelocytic HL-60 leukemic cells and mitogenically stimulated and nonstimulated human lymphocytes
    Tze Chen Hsieh
    Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, NY, USA
    J Altern Complement Med 8:591-602. 2002
    ..Kowloon, Hong Kong) prepared from strain Cov-1 of the mushroom Coriolus versicolor...
  81. pmc Effects of flow and diffusion on chemotaxis studies in a microfabricated gradient generator
    Glenn M Walker
    Vanderbilt Institute for Integrative Biosystems Research and Education, Vanderbilt University, Nashville, TN 37235, USA
    Lab Chip 5:611-8. 2005
    ....
  82. ncbi Differentiated HL-60 cells are a valid model system for the analysis of human neutrophil migration and chemotaxis
    Anna Barbara Hauert
    Department of Pathology, University of Bern, Murtenstr 31, CH 3010 Bern, Switzerland
    Int J Biochem Cell Biol 34:838-54. 2002
    ..In summary, the results presented here show that differentiated HL-60 cells, stimulated with chemotactic peptide, are a valid model system to study molecular mechanisms of neutrophil emigration...
  83. ncbi Anaplasma phagocytophilum specifically induces tyrosine phosphorylation of ROCK1 during infection
    Venetta Thomas
    Section of Rheumatology, Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06520, USA
    Cell Microbiol 9:1730-7. 2007
    ..These data demonstrate a role for A. phagocytophilum-mediated ROCK1 phosphorylation in infection, and suggests that inhibiting this pathway may lead to new, non-antibiotic strategies to treat human granulocytic anaplasmosis...
  84. pmc Sequential analysis of Anaplasma phagocytophilum msp2 transcription in murine and equine models of human granulocytic anaplasmosis
    Diana G Scorpio
    Department of Molecular and Comparative Pathobiology, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Clin Vaccine Immunol 15:418-24. 2008
    ..Alteration in transcription of msp2 has no bearing on clinical disease in horses, suggesting the existence of a separate proinflammatory component differentially expressed with changing in vitro passage...
  85. ncbi Cell proliferation and CD11b expression are controlled independently during HL60 cell differentiation initiated by 1,25 alpha-dihydroxyvitamin D(3) or all-trans-retinoic acid
    M T Drayson
    LRF Differentiation Programme, Division of Immunity and Infection, University of Birmingham, United Kingdom
    Exp Cell Res 266:126-34. 2001
    ..Differentiating agents must therefore regulate the proliferation and the maturation of differentiating myeloid cells by mechanisms that are at least partly independent...
  86. ncbi Mechanism of ADP ribosylation factor-stimulated phosphatidylinositol 4,5-bisphosphate synthesis in HL60 cells
    Alison Skippen
    Department of Physiology, University College London, London WC1E 6JJ, United Kingdom
    J Biol Chem 277:5823-31. 2002
    ..We therefore conclude that both PA derived from the PLD pathway and ARF proteins, by directly activating PIP 5-kinase, contribute to the regulation of PI(4,5)P(2) synthesis at the plasma membrane in HL60 cells...
  87. ncbi 5-Aza-2'-deoxycytidine induces p21WAF expression by demethylation of p73 leading to p53-independent apoptosis in myeloid leukemia
    Karin Schmelz
    Department of Hematology and Oncology, Universitatsmedizin Berlin, Charite, Campus Virchow, Berlin, Germany
    Int J Cancer 114:683-95. 2005
    ..We therefore conclude that p73 is a relevant target for methylation-dependent efficacy of 5-Aza-CdR in AML cells...
  88. ncbi Combination effects of alkylphosphocholines and gemcitabine in malignant and normal hematopoietic cells
    Milka C Georgieva
    Unit of Toxicology and Chemotherapy, German Cancer Research Center, 280 Im Neuenheimer Feld, 69120, Heidelberg, Germany
    Cancer Lett 182:163-74. 2002
    ..Thus, the combination of Gem and an alkylphosphocholine is advantageous in terms of their complementary mode of action, resulting in increased cytotoxicity and lowered myelotoxicity...
  89. ncbi Histone deimination as a response to inflammatory stimuli in neutrophils
    Indira Neeli
    Department of Molecular Sciences, University of Tennessee Health Science Center, Memphis, TN 38163, USA
    J Immunol 180:1895-902. 2008
    ..The possible association of a modified autoantigen with microbial components could, in predisposed individuals, increase the risk of autoimmunity...
  90. ncbi Dicoumarol impairs mitochondrial electron transport and pyrimidine biosynthesis in human myeloid leukemia HL-60 cells
    David González-Aragón
    Departamento de Biologia Celular, Fisiologia e Inmunologia, Facultad de Ciencias, Universidad de Cordoba, Campus Rabanales, Edificio Severo Ochoa, 3 a planta, 14014 Cordoba, Spain
    Biochem Pharmacol 73:427-39. 2007
    ..These actions must be taken into account when considering dicoumarol effects on cells...
  91. ncbi PTEN regulates motility but not directionality during leukocyte chemotaxis
    Rosa Ana Lacalle
    Department of Immunology and Oncology, Centro Nacional de Biotecnologia CSIC, UAM Campus de Cantoblanco, 28049 Madrid, Spain
    J Cell Sci 117:6207-15. 2004
    ..The results suggest a role for PTEN in regulating actin polymerization, but not directionality during mammalian cell chemotaxis...
  92. ncbi Inhibition by vitamin C of apoptosis induced by N-nitrosamines in HepG2 and HL-60 cells
    Nuria Arranz
    Departamento de Nutricion, Bromatologia y Tecnologia de los Alimentos, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, Spain
    J Appl Toxicol 28:788-96. 2008
    ..Therefore, our findings suggest that inhibition of apoptosis may be one of the mechanisms by which vitamin C exerts its protective effect...
  93. ncbi The Leukemia-associated ETO homologues are differently expressed during hematopoietic differentiation
    Sofia Rondin Lindberg
    Department of Hematology, University of Lund, Lund, Sweden
    Exp Hematol 33:189-98. 2005
    ..In conclusion, the differential expression of the ETO homologues suggests that they may have a potential role in hematopoietic differentiation...
  94. pmc The PCH family member proline-serine-threonine phosphatase-interacting protein 1 targets to the leukocyte uropod and regulates directed cell migration
    Kate M Cooper
    Cellular and Molecular Biology Program, University of Wisconsin, Madison, WI 53706, USA
    Mol Biol Cell 19:3180-91. 2008
    ..Together, our findings demonstrate that PSTPIP1 is a novel component of the leukocyte uropod that regulates endocytosis and cell migration...
  95. ncbi Induction of S phase cell arrest and caspase activation by polysaccharide peptide isolated from Coriolus versicolor enhanced the cell cycle dependent activity and apoptotic cell death of doxorubicin and etoposide, but not cytarabine in HL-60 cells
    Kenrie Pui Yan Hui
    Department of Zoology, The University of Hong Kong, Pokfulam Road, Hong Kong, SAR, P R China
    Oncol Rep 14:145-55. 2005
    ..Our data support the potential development of PSP as an adjuvant for leukemia treatment, but also imply the importance of understanding its interaction with individual anticancer agents...
  96. ncbi Synergistic antitumor effects of anthracenylmethyl homospermidine and alpha-difluoromethylornithine on promyelocytic leukemia HL60 cells
    Song Qiang Xie
    Institute of Natural Products and Medicinal Chemistry, Henan University, Kaifeng 475001, China
    Toxicol In Vitro 22:352-8. 2008
    ..All these findings demonstrated that the combination treatment with DFMO and ANTMHspd resulted in synergistic antitumor effects on HL60 cells...
  97. ncbi Proteomic analysis of plasma membrane lipid rafts of HL-60 cells
    Mitsuaki Yanagida
    Institute for Environmental and Gender Specific Medicine, Juntendo University Graduate School of Medicine, Tomioka, Urayasu shi, Chiba, Japan
    Proteomics 7:2398-409. 2007
    ..Identified proteins, expression of which was altered by DMSO treatment, are expected to be candidate proteins involved in differentiation and functions of neutrophils...
  98. ncbi Analysis of neutrophil chemotaxis
    Paul A Nuzzi
    Department of Molecular and Cellular Pharmacology, University of Wisconsin Medical School, Madison, WI, USA
    Methods Mol Biol 370:23-36. 2007
    ..We also discuss methods for HL-60 transfection using retroviral approaches and chemotaxis assays used with differentiated HL-60 cells...
  99. pmc Induction of apoptosis in human leukaemic cells by IPENSpm, a novel polyamine analogue and anti-metabolite
    Alison V Fraser
    Departments of Medicine and Therapeutics and Biomedical Sciences, Polwarth Building, University of Aberdeen, Foresterhill, Aberdeen, AB25 2ZD, Scotland, U K
    Biochem J 367:307-12. 2002
    ..IPENSpm can be classified as a polyamine anti-metabolite and it may be a promising new lead compound in terms of treatment of some human cancers...
  100. ncbi Acteoside inhibits human promyelocytic HL-60 leukemia cell proliferation via inducing cell cycle arrest at G0/G1 phase and differentiation into monocyte
    Kyung Won Lee
    Department of Pharmaceutical Biochemistry, College of Pharmacy, Kyung Hee University, Hoegi dong, Seoul 130 701, Korea
    Carcinogenesis 28:1928-36. 2007
    ..These findings, for the first time, reveal the mechanism underlying the anti-proliferative effect of acteoside on human promyelocytic HL-60 cells...
  101. ncbi Lipid products of PI(3)Ks maintain persistent cell polarity and directed motility in neutrophils
    Fei Wang
    Department of Cellular and Molecular Pharmacology and the Cardiovascular Research Institute, University of California, San Francisco, CA 94143 0450, USA
    Nat Cell Biol 4:513-8. 2002
    ..Thus reciprocal interplay between PtdIns(3,4,5)P(3) and polymerized actin initiates and maintains the asymmetry of intracellular signals responsible for cell polarity and directed motility...

Research Grants63

  1. Prevention of UV-induced carcinogenesis by cyanidin-3-glucoside
    Xianglin Shi; Fiscal Year: 2013
    ..Present study attempts to identify cyanidin-3-glucoside, a plant-derived compound, as a mechanism-based preventive agent against UV-induced skin cancers. ..
  2. HTS for enhancers of neutrophil function that specifically augment PIP3 signal
    Hongbo R Luo; Fiscal Year: 2012
    ..Moreover, the identified activators can be directly utilized as starting chemical compounds for novel immune enhancer drug development, particularly for severe infections due to lack of enough pathogen killing capability. ..
  3. Iron in Mitochondrial Physiology and Disease
    Paul A Lindahl; Fiscal Year: 2012
    ..These iron complexes may also play a role in generating reactive oxygen species (ROS) which are thought to be responsible for some aspects of cellular damage and aging. ..
  4. Small Cell-Penetrating Glyco-Decoys Sidetrack Selectin Ligand Biosynthesis
    Khushi L Matta; Fiscal Year: 2013
    ..This Phase I proposal meets the central tenet of the SBIR program for promising commercial products. ..
  5. CELLULAR UPTAKE AND INTERACTION OF IRON AND GALLIUM
    Christopher Chitambar; Fiscal Year: 1992
    ..35S- methionine and 125I will be used to label and study the synthesis and release of the transferrin receptor from cells. It will be determined if the free transferrin receptor influences gallium cytotoxicity...
  6. PATHOGENESIS OF HUMAN GRANULOCYTIC EHRLICHIOSIS
    Ulrike Munderloh; Fiscal Year: 2001
    ....
  7. FUNCTIONS OF RB PROTEASE(S) IN APOPTOSIS
    QING DOU; Fiscal Year: 2000
    ..These studies should provide insight into the molecular mechanisms of the ppRB degradation, and may provide a basis for the development of novel therapies aimed at activating the ppRB-PTS function in vivo. ..
  8. REGULATION OF ENZYME OF LIPID METABOLISM
    Robert Bell; Fiscal Year: 1990
    ..Alterations of sphingolipid metabolites are likely of importance to the sphingolipidosis, progressive dementias and other neurological disorders, cancer, psoriasis, immune disorders and inflammation...
  9. NON-GENETIC CELL HETEROGENEITY IN TUMOR EVOLUTION
    Sui Huang; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable] [unreadable] [unreadable] [unreadable] [unreadable]..
  10. CELL DEATH INDUCED BY ANTICANCER AGENTS
    Alan Eastman; Fiscal Year: 2007
    ..The ultimate goal of this research program will be to define which pathway and effector a specific leukemia uses, and then prescribe effective drug combinations that will be individualized for that patient. ..
  11. P-21 INDUCTION IN MYELOID DIFFERENTIATION
    Richard Steinman; Fiscal Year: 1999
    ..A clear understanding of p21 function in normal myelopoiesis will establish a context for determining if disruption of p21 function contributes to myelodysplastic syndrome or myeloid leukemias. ..
  12. MECHANISMS OF DRUG ACTION IN TUMOR CELL DIFFERENTIATION
    MICHAEL MORIN; Fiscal Year: 1990
    ....
  13. Role of Nuclear Transport in Acute Leukemia
    NABEEL YASEEN; Fiscal Year: 2004
    ..Looking at entire promoters will provide a global assessment of the effects of Nup98-HOXA9 on myeloid transcription. ..
  14. GLYCOSYLTRANSFERASE GENE EXPRESSION IN TRANSFORMED CELLS
    Nevis Fregien; Fiscal Year: 1992
    ..These probe will also be used to determine the molecular basis of the lesions in the mutant cells lines...
  15. High Throughput Profiling of Plant Extracts Using Engineered Heart Tissues
    Tetsuro Wakatsuki; Fiscal Year: 2006
    ..The ultimate goal of this project is to rapidly and cost-effectively identify an herbal remedy, or compounds from natural products, that could treat cardiovascular diseases. [unreadable] [unreadable] [unreadable]..
  16. GENETIC ELEMENTS CONTROLLING MYELOID DIFFERENTIATION
    Edward Agura; Fiscal Year: 1993
    ..These studies may contribute several important aspects to the understanding of normal and disordered eukaryotic gene regulation in myeloid lineages...
  17. REGULATION OF TUMOR PHENOTYPE BY EXTRACHROMOSOMAL DNA
    S Eckhardt; Fiscal Year: 1999
    ..The data generated from these studies will be used to design novel therapeutic strategies for patients with cancer. ..
  18. PROTEIN KINASE C INHIBITORS AS CHEMOPREVENTIVE AGENTS
    Robert Bell; Fiscal Year: 1991
    ..These studies should create new tools (drugs) which will facilitate acquisition of basic knowledge pertinent to testing the major conceptualizations developed. These drugs may rapidly translate into new chemopreventive agents...
  19. PH REGULATORY EXCHANGE KINETICS IN HL60 CELLS
    Diego Restrepo; Fiscal Year: 1993
    ..The use of these techniques will allow a comprehensive study of intracellular pH regulation in a model system where individual exchanges are well characterized...
  20. FUNCTIONAL HETEROGENEITY OF PROTEIN KINASE C ISOFORMS
    MARIE WOOTEN; Fiscal Year: 1990
    ..This study will provide information regarding differential regulation of PKC isoform expression and a means to examine the functional heterogeneity of this gene family...
  21. Apoptosis in Sepsis: Regulation by TNF & delta-PKC
    Laurie Kilpatrick; Fiscal Year: 2006
    ..4: Assess the role of delta-PKC in regulating the assembly of TNFalpha anti-apoptotic signaling complexes and their association with the p60TNFR. ..
  22. OXIDATIVE EVENTS IN CANCER THERAPY
    Larry Oberley; Fiscal Year: 2005
    ....
  23. NUCLEAR TRANSPORT IN THE GENESIS OF ACUTE LEUKEMIA
    NABEEL YASEEN; Fiscal Year: 1999
    ..The ultimate goal of this research is to determine how translocations involving 'can' lead to leukemia, and hence begin to understand the role of nuclear transport abnormalities in the causation of cancer. ..
  24. VISUALIZATION AND FUNCTION OF MITOCHONDRIAL FORMS
    Thomas James; Fiscal Year: 1980
    ..Work on how the mitochondrial mass changes over the cell cycle in HL60 cells is being carried out using flow-microfluorimetry. The fluorescent dye, rodamine 123, has proved to be highly specific in this study. ..
  25. CELLULAR SIGNALING OF THE CANNABINOID RECEPTOR
    Begonia Ho; Fiscal Year: 1999
    ..The goal is to test whether there is any specificity in receptor -> G protein -> effector coupling, by comparing the signaling mechanisms of two cannabinoid receptors in different cellular environment. ..
  26. Activation of the human neurtrophil
    HELEN KORCHAK; Fiscal Year: 2005
    ..V Establish the role of d-PKC in downregulation of signalling pathways triggered by the p60TNFR. Determine if d-PKC acts on a serine residue(s) in the juxtamembrane region or the death domain. ..
  27. FLUORESCENCE ACTIVATED CELL SORTER
    Andrew Yen; Fiscal Year: 2004
    ..The instrument requested is a Dako-Cytomation basic MoFlo plus a UV-vis, vis, and HeNe laser to provide independent UV, vis/488, and red excitation. ..
  28. Retinoic Acid Regulated Cell Differentiation: BLR1 Rcptr
    Andrew Yen; Fiscal Year: 2005
    ..It has the implication that the ultimate effects of RA can be governed by relevant growth factors signaling through their cognate receptors. ..
  29. Clinical application of multispectral imaging flow cytometry
    Hans Minderman; Fiscal Year: 2008
    ..The aim of the proposed studies is to determine necessary adjustments to this instrument's hardware and analysis software to accurately and efficiently use it in a clinical research setting. ..
  30. TOPOISOMERASES IN RETINOID INDUCED DIFFERENTIATION
    Andrew Yen; Fiscal Year: 2003
    ..Andrew Yen, who brings expertise on the molecular and cellular biology of retinoic acid-induced myeloid differentiation. ..
  31. Innate Effectors of rAls3p-N Anti-Candida Vaccine
    Brad J Spellberg; Fiscal Year: 2010
    ..PNS 398/2590 (Rev. 11/07) Page - Continuation Format Page ..
  32. Tyrosine phosphorylation during A. phagocytophilum invasion
    Jacob Ijdo; Fiscal Year: 2008
    ..unreadable] [unreadable] In the Unites States, Human Granulocytic Ehrlichiosis is an emerging tick-associated disease and in[unreadable] endemic areas the incidence is second only to Lyme disease. ..
  33. COPPER/ALBUMIN REDOX-CYCLING IN PREECLAMPSIA
    Valerian Kagan; Fiscal Year: 2003
    ....
  34. Irradiation Damage and Protection of Pulmonary Endothelium Oxidative Lipidomics
    Valerian E Kagan; Fiscal Year: 2010
    ....
  35. Adrenergic Antagonism During Murine Candidiasis
    Brad Spellberg; Fiscal Year: 2008
    ..For these reasons, the potential for adrenergic antagonism to act as an effective immunotherapy is highly meritorious of investigation in this training application. ..
  36. ACOUSTICALLY ACTIVATED MICELLAR DRUG DELIVERY
    Natalya Rapoport; Fiscal Year: 2007
    ..abstract_text> ..
  37. VITAMIN D PLANT ANTIOXIDANT SILIBININ IN LEUKEMIA TRANSLATIONAL STUDY
    George P Studzinski; Fiscal Year: 2010
    ..Thus, myeloid leukemia can be considered an orphan disease, and merits support from public sources for the development of novel therapy. ..
  38. IGG PLACENTAL TRANSPORT: ENDOTHELIAL CAVEOLAE
    John Robinson; Fiscal Year: 2004
    ..Our approaches to testing the predictions of this hypothesis incorporate methods of cell biology, immunology, biochemistry, ultrastructural analysis, and molecular biology. ..
  39. Vaccine hurdle to anthrax and the emerging immune system
    Pranela Rameshwar; Fiscal Year: 2006
    ..This study will also form the basis to develop in vivo model with human hematopoietic system to study anthrax infection and also to improve responses to anthrax vaccines. ..
  40. Characterization of A Novel 65kDa E-selectin Ligand on G-CSF Mobilized Leukocytes
    Robert Sackstein; Fiscal Year: 2008
    ..It is anticipated that the results obtained will lead to new approaches for preventing or alleviating G-CSF-induced vascular and inflammatory complications [unreadable] [unreadable] [unreadable]..
  41. Microenvironmental mechanisms of leukemia cell survival
    Richard Larson; Fiscal Year: 2009
    ..In addition, we will share this data with Dr. Tom Look at the Dana Farber Cancer Center in order to correlate his genetic findings in his T-ALL Zebra fish model with our human studies. ..
  42. Microscale control of stem cell signaling using cell patterning and perfusion
    Joel Voldman; Fiscal Year: 2010
    ..Our technology provides a new window into stem cell biology that will help determine new ways to control their behavior. ..
  43. Role of Polyamines in MYCN-Amplified Neuroblastoma
    ANDRE BACHMANN; Fiscal Year: 2009
    ..Our research will be useful in the development of novel treatments for the most aggressive forms of the childhood cancer neuroblastoma. ..
  44. Implications of Retinoid-Induced CD38 Antigen Expression
    Kapil Mehta; Fiscal Year: 2006
    ..unreadable] [unreadable]..
  45. G protein signal integration by multifunctional proteins
    T Harden; Fiscal Year: 2006
    ..abstract_text> ..
  46. AIRWAY SMOOTH MUSCLE IN HEALTH AND DISEASE
    RONALD COBURN; Fiscal Year: 2004
    ..In particular, all of these diseases cause physiological alterations that influence the length at which smooth muscle cells contract and relax. ..
  47. Chemoprevention of prostate cancer by resveratrol
    Tze Chen Hsieh; Fiscal Year: 2005
    ....
  48. BIOEFFECTS OF GAS BODY ACTIVATION IN MEDICAL ULTRASOUND
    Douglas Miller; Fiscal Year: 2001
    ..abstract_text> ..
  49. BREAST CANCER/BONE MARROW STROMAL INTERACTIONS
    Pranela Rameshwar; Fiscal Year: 2003
    ..Since BC metastasis involves two major cell subsets, mesenchymal (stroma) and epithelial (BC), aim 4 will determine if regulation of PPT-I, NK-1 and NK-2 is tissue specific by studying their promoters in these cell subsets. ..
  50. Structure & Biology of Hematopoietic E-/L-Selectin
    Robert Sackstein; Fiscal Year: 2006
    ..This information will contribute greatly to our understanding of the adhesive systems that regulate steady-state and pathologic hematopoiesis, as well as hematopoietic recovery following stem cell transplantation. ..
  51. Identification of Anaplasma phagocytophilum adhesins
    JASON CARLYON; Fiscal Year: 2006
    ..Furthermore, it will identify novel targets for preventing and treating HGA and may pave the way for development of pharmacologic inhibitors of cellular adhesion events associated with disease. ..
  52. Actinobacillus actinomycetemcomitans Leukotoxin Induced Cell Death
    Edward Lally; Fiscal Year: 2007
    ..abstract_text> ..
  53. Preclinical Evaluation of Bcr/Abl-Directed Tyrphostins
    Scott Kaufmann; Fiscal Year: 2007
    ..abstract_text> ..
  54. Regulation of gp91phox by Anaplasma phagocytophila
    Venetta Thomas; Fiscal Year: 2007
    ..Aim 2. To define the effect of A. phagocytophila infection on the activity of the activator proteins and their binding capacity of the gp91phox promoter. ..
  55. Opiate's Effects on the Inflammation and Cytotoxicity in HIV-1 Transgenic Rat
    SULIE CHANG; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  56. Isothiocyanates in the Chemoprevention of Bladder Cancer
    Yuesheng Zhang; Fiscal Year: 2007
    ..Aim 5 will determine the efficacy of an orally administered ITC in inhibiting N-butyl-N- (4-hydroxybutyl)-nitrosamine-induced bladder tumorigenesis in F344 rats. ..
  57. Annual Midwest DNA Repair Symposium
    Altaf Wani; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable] [unreadable]..
  58. NUCLEAR DIFFERENTATION DURING MURINE GRANULOPOIESIS
    DONALD OLINS; Fiscal Year: 2004
    ..abstract_text> ..
  59. Antisense Mediated Suppression of Dystrophin Mutations
    Michael Howard; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  60. PHOSPHATIDYLSERINE-SPECIFIC RECEPTOR FOR APOPTOTIC CELLS
    Valerie Fadok; Fiscal Year: 2004
    ..The investigators hypothesize that PS recognition has been preserved throughout phylogeny as an effective mechanism mediating the removal of apoantibodies. ..
  61. Role of Lamin B Receptor in Neutrophil Maturation and Functional Activation
    Peter Gaines; Fiscal Year: 2007
    ..The results may also offer important insight into the pathogenesis of potentially fatal hematologic disorders. [unreadable] [unreadable] [unreadable]..