Genomes and Genes
hl 60 cells
Summary: A promyelocytic cell line derived from a patient with ACUTE PROMYELOCYTIC LEUKEMIA. HL-60 cells lack specific markers for LYMPHOID CELLS but express surface receptors for FC FRAGMENTS and COMPLEMENT SYSTEM PROTEINS. They also exhibit phagocytic activity and responsiveness to chemotactic stimuli. (From Hay et al., American Type Culture Collection, 7th ed, pp127-8)
Publications288 found, 100 shown here
- Prevention of apoptosis by Bcl-2: release of cytochrome c from mitochondria blockedJ Yang
Department of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322, USA
Science 275:1129-32. 1997..Overexpression of Bcl-2 prevented the efflux of cytochrome c from the mitochondria and the initiation of apoptosis. Thus, one possible role of Bcl-2 in prevention of apoptosis is to block cytochrome c release from mitochondria...
- Superoxide dismutase as a target for the selective killing of cancer cellsP Huang
Department of Experimental Therapeutics, The University of Texas M D Anderson Cancer Center, Houston 77030, USA
Nature 407:390-5. 2000..Our results indicate that targeting SOD may be a promising approach to the selective killing of cancer cells, and that mechanism-based combinations of SOD inhibitors with free-radical-producing agents may have clinical applications...
- Cell fates as high-dimensional attractor states of a complex gene regulatory networkSui Huang
Vascular Biology Program, Departments of Pathology and Surgery, Children s Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA
Phys Rev Lett 94:128701. 2005....
- Rac and Cdc42 play distinct roles in regulating PI(3,4,5)P3 and polarity during neutrophil chemotaxisSupriya Srinivasan
Department of Cellular and Molecular Pharmacology and the Cardiovascular Research Institute, University of California, San Francisco, CA 94143, USA
J Cell Biol 160:375-85. 2003..We conclude that Rac plays a dominant role in the PI(3,4,5)P3-dependent positive feedback loop required for forming a leading edge, whereas location and stability of the leading edge are regulated by Cdc42...
- Anaplasma phagocytophilum AnkA secreted by type IV secretion system is tyrosine phosphorylated by Abl-1 to facilitate infectionMingqun Lin
Department of Veterinary Biosciences, The Ohio State University, 1925 Coffey Road, Columbus, OH 43210, USA
Cell Microbiol 9:2644-57. 2007....
- A PtdInsP(3)- and Rho GTPase-mediated positive feedback loop regulates neutrophil polarityOrion D Weiner
Department of Cell Biology, Harvard Medical School and Beth Israel Deaconess Medical Center, 240 Longwood Ave C 1, Boston, MA 02115, USA
Nat Cell Biol 4:509-13. 2002..Emergent properties of positive and negative regulatory links between PtdInsP(3) and Rho family GTPases may constitute a broadly conserved module for the establishment of cell polarity during eukaryotic chemotaxis...
- Thymoquinone induces apoptosis through activation of caspase-8 and mitochondrial events in p53-null myeloblastic leukemia HL-60 cellsMohamed A El-Mahdy
Department of Radiology, Ohio State University, Columbus, OH 43210, USA
Int J Cancer 117:409-17. 2005..Activated caspase-8 initiates the release of cytochrome c during TQ-induced apoptosis. Overall, these results offer a potential mechanism for TQ-induced apoptosis in p53-null HL-60 cancer cells...
- Functional characterization of mitochondria in neutrophils: a role restricted to apoptosisN A Maianski
Emma Childrens Hospital, Academic Medical Center, University of Amsterdam, The Netherlands
Cell Death Differ 11:143-53. 2004..We suggest that other proapoptotic factors such as Smac/DIABLO and HtrA2/Omi, which are massively released from the mitochondria, have an important role in neutrophil apoptosis...
- The antitumor ether lipid ET-18-OCH(3) induces apoptosis through translocation and capping of Fas/CD95 into membrane rafts in human leukemic cellsC Gajate
, , CSIC-Universidad de Salamanca, Campus Miguel de Unamuno, Salamanca, Spain
Blood 98:3860-3. 2001..This translocation of Fas into membrane rafts may provide a mechanism for amplifying Fas signaling by reorganization of membrane microdomains...
- Deimination of arginine residues in nucleophosmin/B23 and histones in HL-60 granulocytesTeruki Hagiwara
Graduate School of Integrated Science, Yokohama City University, 22 2, Seto, Kanazawa Ku, Yokohama, 236 0027, Japan
Biochem Biophys Res Commun 290:979-83. 2002..The citrulline content of histones amounted to about 10% of the histone molecules. We discuss the implications of deimination of these proteins for their nuclear functions...
- Human PAD4 regulates histone arginine methylation levels via demethyliminationYanming Wang
Department of Genetic Medicine, Weill Medical College of Cornell University, 1300 York Avenue, New York, NY 10021, USA
Science 306:279-83. 2004..Moreover, PAD4 activity is linked with the transcriptional regulation of estrogen-responsive genes in MCF-7 cells. These data suggest that PAD4 mediates gene expression by regulating Arg methylation and citrullination in histones...
- Arsenic trioxide selectively induces acute promyelocytic leukemia cell apoptosis via a hydrogen peroxide-dependent pathwayY Jing
Rochelle Belfer Chemotherapy Foundation Laboratory, Division of Neoplastic Diseases, Department of Medicine, Mount Sinai Medical Center, New York, NY, USA
Blood 94:2102-11. 1999....
- Resveratrol arrests the cell division cycle at S/G2 phase transitionF D Ragione
Medical School, Second University of Naples, Naples, Italy
Biochem Biophys Res Commun 250:53-8. 1998..These data demonstrate that resveratrol causes a complete and reversible cell cycle arrest at the S phase checkpoint...
- Role of NADPH oxidase in arsenic-induced reactive oxygen species formation and cytotoxicity in myeloid leukemia cellsWen Chien Chou
Program of Human Genetics and Molecular Biology, School of Medicine, Johns Hopkins University, Baltimore, MD 21205, USA
Proc Natl Acad Sci U S A 101:4578-83. 2004..Our findings pinpoint the arsenic target of ROS production and provide a conceptual basis for an anticancer regimen...
- Activation of extracellular signal-regulated kinases (ERKs) defines the first phase of 1,25-dihydroxyvitamin D3-induced differentiation of HL60 cellsX Wang
Department of Pathology and Laboratory Medicine, UMDNJ-New Jersey Medical School, 185 South Orange Avenue, Newark, New Jersey 07103, USA
J Cell Biochem 80:471-82. 2001....
- A role for oxidative stress in apoptosis: oxidation and externalization of phosphatidylserine is required for macrophage clearance of cells undergoing Fas-mediated apoptosisValerian E Kagan
Department of Environmental and Occupational Health, University of Pittsburgh, Pittsburgh, PA 15260, USA
J Immunol 169:487-99. 2002..Taken together, our data suggest that the presence of PS-OX in conjunction with nonoxidized PS on the cell surface is an important signal for macrophage clearance of apoptotic cells...
- Expression of the miR-17-92 polycistron in chronic myeloid leukemia (CML) CD34+ cellsLetizia Venturini
Department of Hematology, Hemostasis, and Oncology, Hannover Medical School, Germany
Blood 109:4399-405. 2007..Altered miRNA expression may contribute to the pathophysiology of the disease and may provide potential targets for therapeutic intervention...
- The Vav binding site of the non-receptor tyrosine kinase Syk at Tyr 348 is critical for beta2 integrin (CD11/CD18)-mediated neutrophil migrationJurgen Schymeinsky
Department of Physiology, Ludwig Maximilians Universitat Munchen, Schillerstr 44, D 80336 MUnchen, Germany
Blood 108:3919-27. 2006..These findings indicate that Syk is critical for beta(2) integrin-mediated neutrophil migration in vitro and plays a fundamental role in neutrophil recruitment during the inflammatory response in vivo...
- Effect of cytarabine and decitabine in combination in human leukemic cell linesTaichun Qin
Department of Leukemia, The University of Texas M D Anderson Cancer Center, Houston, Texas 77030, USA
Clin Cancer Res 13:4225-32. 2007..5-Aza-2'-deoxycytidine (DAC) is a cytosine analogue that inhibits DNA methylation and also has activity in myeloid leukemia. Therefore, we investigated combining these two drugs in human leukemia cell lines in vitro...
- The human EGF-TM7 receptor EMR3 is a marker for mature granulocytesMourad Matmati
Department of Experimental Immunology, Academic Medical Center, University of Amsterdam, 1100 DE Amsterdam, The Netherlands
J Leukoc Biol 81:440-8. 2007..These results demonstrate that the expression of EGF-TM7 receptors on myeloid cells is differentially regulated. EMR3 is the first family member found mainly on granulocytes...
- Antiapoptotic action of focal adhesion kinase (FAK) against ionizing radiationTadashi Kasahara
Department of Biochemistry, Kyoritsu College of Pharmacy, Shibakoen 1 5 30, Minato ku, Tokyo, 105 8512, Japan
Antioxid Redox Signal 4:491-9. 2002..These results indicated that mitochondria pathway is required for IR-induced apoptosis, and FAK overexpression prevents this pathway, thus rendering antiapoptotic states...
- Loss of phospholipid asymmetry and surface exposure of phosphatidylserine is required for phagocytosis of apoptotic cells by macrophages and fibroblastsV A Fadok
Program in Cell Biology, Department of Pediatrics, National Jewish Medical and Research Center, Denver, Colorado 80206, USA
J Biol Chem 276:1071-7. 2001..These observations directly demonstrate that loss of phospholipid asymmetry and PS expression is required for phagocyte engulfment of apoptotic cells and imply a critical, if not obligatory, role for PS recognition in the uptake process...
- Down-regulation of the mitochondrial translation system during terminal differentiation of HL-60 cells by 12-O-tetradecanoyl-1-phorbol-13-acetate: comparison with the cytoplasmic translation systemNono Takeuchi
Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Building FSB 401, 5 1 5, Kashiwanoha, Kashiwa, Chiba Prefecture 277 8562, Japan
J Biol Chem 278:45318-24. 2003....
- Phosphatidylinositol transfer protein beta displays minimal sphingomyelin transfer activity and is not required for biosynthesis and trafficking of sphingomyelinBruno Segui
Department of Physiology, University College London, London WC1E 6JJ, U K
Biochem J 366:23-34. 2002..Altogether, these observations do not support the suggestion that PITPbeta participates in the transfer of SM, the regulation of SM biosynthesis or its intracellular trafficking...
- Anaplasma phagocytophilum delays spontaneous human neutrophil apoptosis by modulation of multiple apoptotic pathwaysYan Ge
Department of Veterinary Biosciences, The Ohio State University, Columbus, 43210, USA
Cell Microbiol 8:1406-16. 2006..These data point to a novel mechanism induced by A. phagocytophilum involving both extrinsic and intrinsic pathways to ensure to delay the apoptosis of host neutrophils...
- Divergent signals and cytoskeletal assemblies regulate self-organizing polarity in neutrophilsJingsong Xu
Department of Cellular and Molecular Pharmacology and the Cardiovascular Research Institute, University of California, San Francisco, San Francisco, CA 94143, USA
Cell 114:201-14. 2003..The latter effect explains both the neutrophil's ability to polarize in uniform concentrations of chemoattractant and its response to reversal of an attractant gradient by performing a U-turn...
- Enhancement of antineoplastic action of 5-aza-2'-deoxycytidine by phenylbutyrate on L1210 leukemic cellsMaryse Lemaire
Department de Pharmacologie, Universite de Montreal, Centre de Recherche Pediatrique, Hopital Sainte Justine, 3175 Cote Sainte Catherine, Montreal, Quebec H3T 1C5, Canada
Leuk Lymphoma 45:147-54. 2004..We also observed an enhancement of the antineoplastic activity of this combination in mice with L1210 leukemia. These data provide a rationale to investigate 5-AZA-CdR and PB in patients with advanced leukemia...
- Promyelocytic HL60 cells express NADPH oxidase and are excellent targets in a rapid spectrophotometric microplate assay for extracellular superoxideOlga Teufelhofer
Institute of Cancer Research, Division Oncological Toxicology, University of Vienna, Austria
Toxicol Sci 76:376-83. 2003....
- Microtubule damaging agents induce apoptosis in HL 60 cells and G2/M cell cycle arrest in HT 29 cellsChia Jen Tseng
Graduate Institute of Biomedical Technology, Taipei Medical University, No 250, Wu Hsing Street, Taipei 110, Taiwan, ROC
Toxicology 175:123-42. 2002..increased leakage of cytochrome c from mitochondria into cytosolic fraction were detected in the PDP-treated HL 60 cells. These listed intracellular events were interpreted to lead to the apoptosis observed in PDP-treated HL 60 ..
- Acoustic activation of drug delivery from polymeric micelles: effect of pulsed ultrasoundA Marin
Department of Bioengineering, University of Utah, Salt Lake City, UT 84112, USA
J Control Release 71:239-49. 2001..The second mechanism is based on the perturbation of cell membranes that results in the increased uptake of the micellar-encapsulated drug. The intracellular uptake of Pluronic micelles was confirmed by fluorescence microscopy...
- Arginine methylation provides epigenetic transcription memory for retinoid-induced differentiation in myeloid cellsBalint L Balint
Department of Biochemistry and Molecular Biology, Research Center for Molecular Medicine, University of Debrecen, Medical and Health Science Center, Nagyerdei krt 98, Debrecen H 4012, Hungary
Mol Cell Biol 25:5648-63. 2005....
- Neutrophil maturation and the role of retinoic acidN D Lawson
Department of Biology, Yale University School of Medicine, New Haven, CT 06510, USA
Exp Hematol 27:1355-67. 1999..Here we present a review of the mechanism of retinoic acid receptor action and the major evidence showing that normal retinoid signaling is required for neutrophil maturation...
- Translational control of specific genes during differentiation of HL-60 cellsA M Krichevsky
Department of Molecular Virology, The Faculty of Medicine, Hebrew University of Jerusalem, P O Box 12272, Jerusalem 91120, Israel
J Biol Chem 274:14295-305. 1999....
- c-MYC interacts with INI1/hSNF5 and requires the SWI/SNF complex for transactivation functionS W Cheng
Department of Molecular Genetics, Albert Einstein College of Medicine, Bronx, New York 10461, USA
Nat Genet 22:102-5. 1999..Our results suggest that the SWI/SNF complex is necessary for c-MYC-mediated transactivation and that the c-MYC-INI1 interaction helps recruit the complex...
- Neutrophil microtubules suppress polarity and enhance directional migrationJingsong Xu
Department of Cellular and Molecular Pharmacology and Cardiovascular Research Institute, University of California, San Francisco, CA 94143 0450, USA
Proc Natl Acad Sci U S A 102:6884-9. 2005..Thus, microtubules normally balance backness vs. frontness signals, preventing backness from reducing the strength of pseudopods and from impairing directional migration...
- Leukocyte adhesion to vascular endothelium induces E-selectin linkage to the actin cytoskeletonM Yoshida
Department of Pathology, Brigham and Women s Hospital, Boston, MA 02115, USA
J Cell Biol 133:445-55. 1996....
- A hematopoietic cell L-selectin ligand that is distinct from PSGL-1 and displays N-glycan-dependent binding activityR Sackstein
Bone Marrow Transplant Service, Department of Medicine, Massachusetts General Hospital, Boston, MA, USA
Blood 96:2765-74. 2000..This novel ligand may help mediate L-selectin-dependent cell-cell adhesive interactions within the cytoarchitecture of the bone marrow microenvironment. (Blood. 2000;96:2765-2774)..
- Thalidomide inhibits growth of tumors through COX-2 degradation independent of antiangiogenesisGang jun Du
Department of Pharmacology, Pharmacy College of He nan University, Kaifeng 475001, He Nan, China
Vascul Pharmacol 43:112-9. 2005..Effect of thalidomide on COX-1 and COX-2 in vivo was consistent with that of in vitro. These results demonstrated that thalidomide might inhibit growth of tumors through COX-2 degradation independent of antiangiogenesis...
- Differential gene expression in retinoic acid-induced differentiation of acute promyelocytic leukemia cells, NB4 and HL-60 cellsKi Hwan Lee
Department of Biochemistry, College of Medicine, Hanyang University, Seoul, Republic of Korea
Biochem Biophys Res Commun 296:1125-33. 2002..Further studies will be needed to define the important molecular pathways involved in granulocytic differentiation by ATRA in APL cells...
- Regulation of NRAMP1 gene expression by 1alpha,25-dihydroxy-vitamin D(3) in HL-60 phagocytesE A Roig
INRS Institut Armand Frappier, 531 Boulevard des Prairies, Laval, H7V 1B7 Quebec, Canada
J Leukoc Biol 71:890-904. 2002..Thus, VD genomic effects stimulate NRAMP1 transcription and protein expression in maturing phagocytes...
- Molecular mechanism of violacein-mediated human leukemia cell deathCarmen Verissima Ferreira
Departamento de Bioquimica, Universidade Estadual de Campinas, Sao Paulo, Brazil
Blood 104:1459-64. 2004..Hence, violacein represents the first member of a novel class of cytotoxic drugs mediating apoptosis of HL60 cells by way of the specific activation of TNF receptor 1...
- Hydroquinone-induced apoptosis in HL-60 cellsHiroshi Terasaka
Department of Oral Diagnosis, Meikai University School of Dentistry, Saitama, Japan
Anticancer Res 25:161-70. 2005..The activation of caspase-3 by HQ/NAC combinations suggests that NAC, a precursor of intracellular glutathione synthesis, acts as a co-catalyst during HQ-induced apoptosis...
- DNA repair contributes to the drug-resistant phenotype of primary acute myeloid leukaemia cells with FLT3 internal tandem duplications and is reversed by the FLT3 inhibitor PKC412C H Seedhouse
Department of Academic Haematology, School of Molecular Medical Sciences, Nottingham University Hospitals and University of Nottingham, Nottingham, UK
Leukemia 20:2130-6. 2006....
- Dihydroartemisinin induces apoptosis in HL-60 leukemia cells dependent of iron and p38 mitogen-activated protein kinase activation but independent of reactive oxygen speciesJin Jian Lu
Division of Anti tumor Pharmacology, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, P R China
Cancer Biol Ther 7:1017-23. 2008..Altogether, our data uncover that DHA induces apoptosis is dependent of iron and p38 MAPK activation but not ROS in HL-60 cells...
- The acute-phase protein alpha 1-acid glycoprotein (AGP) induces rises in cytosolic Ca2+ in neutrophil granulocytes via sialic acid binding immunoglobulin-like lectins (siglecs)Peter Gunnarsson
Division of Pharmacology, Department of Medicine and Care, Linkoping University, S 581 85 Linkoping, Sweden
FASEB J 21:4059-69. 2007..Our report provides the first evidence for a signaling capacity by AGP through a defined receptor. Pre-engagement of L-selectin significantly enhanced this signaling capacity...
- Embelin derivatives and their anticancer activity through microtubule disassemblyMinjuan Xu
State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Xueyuan Road 38, 100083 Beijing, P R China
Planta Med 71:944-8. 2005..Treatment with 10 microM of 1 and 2 for 24 h induced apoptosis in HL-60 cells. This evidence suggests that both 1 and 2 are promising novel antimitotic and anticancer molecules targeting microtubular proteins...
- Multistable and multistep dynamics in neutrophil differentiationHannah H Chang
Vascular Biology Program, Department of Pathology and Surgery, Children s Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA
BMC Cell Biol 7:11. 2006..However, the dynamics of mammalian cell differentiation has not been analyzed with respect to bistability...
- Zosuquidar restores drug sensitivity in P-glycoprotein expressing acute myeloid leukemia (AML)Ruoping Tang
INSERM, U872 Equipe 18 Paris, F 75006 France
BMC Cancer 8:51. 2008..In this paper, the capacity of zosuquidar, a specific P-gp modulator, to reverse chemoresistance was examined in both leukemia cell lines and primary AML blasts...
- Identification of Ki23819, a highly potent inhibitor of kinase activity of mutant FLT3 receptor tyrosine kinaseY Komeno
Department of Hematology and Oncology, Graduate School of Medicine, University of Tokyo, Tokyo 113 8655, Japan
Leukemia 19:930-5. 2005..FLT3-ITD-dependent activation of the downstream signaling proteins ERK and STAT5 was also inhibited within similar concentration ranges. Thus, Ki23819 is a potent in vitro inhibitor of FLT3...
- Impact of cholesterol depletion on shape changes, actin reorganization, and signal transduction in neutrophil-like HL-60 cellsVerena Niggli
Department of Pathology, University of Bern, CH 3010 Bern, Switzerland
Exp Cell Res 296:358-68. 2004....
- Retinoic acid treated HL60 cells express CEACAM1 (CD66a) and phagocytose Neisseria gonorrhoeaeMilica Pantelic
Department of Microbiology and Immunology, Division of Infectious Diseases, Walther Oncology Center, Indiana University School of Medicine, Indianapolis, IN, USA
FEMS Immunol Med Microbiol 42:261-6. 2004..Our results indicate that the level of expression of CEACAM1 in HL60 cells can be regulated by treatment with RA in a differentiation-dependent manner, and that this is important for phagocytosis of OpaI-expressing gonococci or E. coli...
- Nuclear envelope and chromatin compositional differences comparing undifferentiated and retinoic acid- and phorbol ester-treated HL-60 cellsA L Olins
Foundation for Blood Research, P O Box 190, 69 U S Route One, Scarborough, Maine 04070 0190, USA
Exp Cell Res 268:115-27. 2001....
- Active extracts of wild fruiting bodies of Antrodia camphorata (EEAC) induce leukemia HL 60 cells apoptosis partially through histone hypoacetylation and synergistically promote anticancer effect of trichostatin AMei Chin Lu
Graduate Institute of Natural Products, School of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan
Arch Toxicol 83:121-9. 2009..We found that the ethanol extract from wild fruiting bodies of Antrodia camphorata (EEAC) could induce HL 60 cells apoptosis via histone hypoacetylation, up-regulation of histone deacetyltransferase 1 (HDAC 1), and down-..
- Insights into pathogen immune evasion mechanisms: Anaplasma phagocytophilum fails to induce an apoptosis differentiation program in human neutrophilsDori L Borjesson
Department of Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, St Paul, MN 55108, USA
J Immunol 174:6364-72. 2005..phagocytophilum fails to trigger the apoptosis differentiation program usually induced by bacteria, and 2) a protein or molecule on the pathogen surface can mediate an early delay in spontaneous neutrophil apoptosis...
- Orderly pattern of development of the autoantibody response in (New Zealand White x BXSB)F1 lupus mice: characterization of target antigens and antigen spreading by two-dimensional gel electrophoresis and mass spectrometrySandrine Thébault
Institut de la Santé et de la Recherche Médicale Unité 519, Faculte de Medecine et de Pharmacie, Hopital Charles Nicolle, Rouen, France
J Immunol 169:4046-53. 2002....
- Molecular characterization of peptidylarginine deiminase in HL-60 cells induced by retinoic acid and 1alpha,25-dihydroxyvitamin D(3)K Nakashima
Graduate School of Integrated Science, Yokohama City University, 22 2, Seto, Kanazawa Ku, Yokohama 236 0027, Japan
J Biol Chem 274:27786-92. 1999..Neither the enzyme activity nor the protein was found in macrophage-induced cells. These results suggested that expression of the PAD gene is tightly linked to myeloid differentiation...
- Nuclear localization of peptidylarginine deiminase V and histone deimination in granulocytesKatsuhiko Nakashima
Graduate School of Integrated Science, Yokohama City University, 22 2 Seto, Kanazawa Ku, Yokohama 236 0027, Japan
J Biol Chem 277:49562-8. 2002..The major deiminated proteins were identified as histones H2A, H3, and H4. The implication of PAD V in histone modifications is discussed...
- N-hydroxy-N'-(3,4,5-trimethoxyphenyl)-3,4,5-trimethoxy-benzamidine, a novel resveratrol analog, inhibits ribonucleotide reductase in HL-60 human promyelocytic leukemia cells: synergistic antitumor activity with arabinofuranosylcytosinePhilipp Saiko
Clinical Institute of Medical and Chemical Laboratory Diagnostics, Medical University of Vienna, General Hospital of Vienna, Waehringer Guertel 18 20, A 1090 Vienna, Austria
Int J Oncol 31:1261-6. 2007..In addition, KITC exhibited synergistic combination effects when applied sequentially with Ara-C. Due to these promising results, KITC deserves further preclinical and in vivo testing...
- Cytotoxic and biochemical effects of 3,3',4,4',5,5'-hexahydroxystilbene, a novel resveratrol analog in HL-60 human promyelocytic leukemia cellsZsuzsanna Horvath
Clinical Institute of Medical and Chemical Laboratory Diagnostics, General Hospital of Vienna, Medical University of Vienna, Vienna, Austria
Exp Hematol 34:1377-84. 2006..In the present study, the activity of a novel RV analog, 3,3',4,4',5,5'-hexahydroxystilbene (M8), was investigated in HL-60 human promyelocytic leukemia cells...
- Synergistic action of resveratrol, an ingredient of wine, with Ara-C and tiazofurin in HL-60 human promyelocytic leukemia cellsZsuzsanna Horvath
Clinical Institute of Medical and Chemical Laboratory Diagnostics, General Hospital of Vienna, Medical University of Vienna, Vienna, Austria
Exp Hematol 33:329-35. 2005....
- Gallic acid inhibits ribonucleotide reductase and cyclooxygenases in human HL-60 promyelocytic leukemia cellsSibylle Madlener
Clinical Institute of Medical and Chemical Laboratory Diagnostics, Medical University of Vienna, General Hospital of Vienna, Waehringer Guertel 18 20, A 1090 Vienna, Austria
Cancer Lett 245:156-62. 2007..Taken together, we identified novel biochemical effects of GA which could be the basis for further preclinical and in vivo studies...
- Comparison of the gene expression profiles of monocytic versus granulocytic lineages of HL-60 leukemia cell differentiation by DNA microarray analysisJu Han Song
College of Pharmacy and Research Institute of Drug Development, Chonnam National University, Kwangju, South Korea
Life Sci 73:1705-19. 2003..The identification of genes associated with a specific differentiation lineage may give important insights into functional and phenotypic differences between two lineages of HL-60 cell differentiation...
- Nanocytotoxicity: violacein and violacein-loaded poly (D, L-lactide-co-glycolide) nanoparticles acting on human leukemic cellsPatricia S Melo
Biochemistry Department, Instituto de Biologia, Universidade Estadual de Campinas UNICAMP, CP 6109, Campinas, SP, 13083 970, Brazil
J Biomed Nanotechnol 5:192-201. 2009..These methodologies are promising as diagnostic and mechanistic effects of nanoparticles in cell cultures...
- To stabilize neutrophil polarity, PIP3 and Cdc42 augment RhoA activity at the back as well as signals at the frontAlexandra Van Keymeulen
Department of Cellular and Molecular Pharmacology, and Cardiovascular Research Institute, University of California, San Francisco, San Francisco, CA 94143, USA
J Cell Biol 174:437-45. 2006..Thus, PIP3 and Cdc42 maintain stable polarity with a single front and a single back not only by strengthening pseudopods but also, at longer range, by promoting RhoA-dependent actomyosin contraction at the trailing edge...
- Peribysins A-D, potent cell-adhesion inhibitors from a sea hare-derived culture of Periconia speciesTakeshi Yamada
Osaka University of Pharmaceutical Sciences, 4 20 1, Nasahara, Takatsuki, Osaka 569 1094, Japan
Org Biomol Chem 2:2131-5. 2004..All these metabolites potently inhibited the adhesion of human-leukemia HL-60 cells to HUVEC. The activity of compound 4, exhibiting the most potent inhibitory activity, was 380 times as potent as herbimycin A (standard)...
- Differential cell fates induced by all-trans retinoic acid-treated HL-60 human leukemia cellsMunetaka Ozeki
Division of Immunology, Beckman Research Institute of the City of Hope, 1500E Duarte Rd, Duarte, CA 91010, USA
J Leukoc Biol 84:769-79. 2008..These studies demonstrate distinct cell fates for ATRA-treated HL-60 cells that provide new insights into ATRA-induced cell differentiation...
- Optimization of nb-4 and hl-60 differentiation for use in opsonophagocytosis assaysRoland A Fleck
Division of Cell Biology and Imaging, National Institute for Biological Standards and Control, Blanche Lane, South Mimms, Potters Bar, Hertfordshire, United Kingdom EN6 3QG
In Vitro Cell Dev Biol Anim 39:235-42. 2003..Our results indicated that ATRA supplemented with vitamin D(3) and granulocyte colony-stimulating factor affords robust, rapid, and reproducible differentiation of both cell types...
- Analysis of Myc bound loci identified by CpG island arrays shows that Max is essential for Myc-dependent repressionDaniel Y L Mao
Ontario Cancer Institute Princess Margaret Hospital, University of Toronto, Ontario M5G 2M9, Canada
Curr Biol 13:882-6. 2003..Taken together, the identification and analysis of Myc bound target genes supports a model whereby Max plays an essential and universal role in the mechanism of Myc-dependent transcriptional regulation...
- Differential expression of VirB9 and VirB6 during the life cycle of Anaplasma phagocytophilum in human leucocytes is associated with differential binding and avoidance of lysosome pathwayHua Niu
Department of Veterinary Biosciences, The Ohio State University, Columbus, OH 43210, USA
Cell Microbiol 8:523-34. 2006..phagocytophilum intracellular life cycle and suggest that bacterial developmental stages influence the nature of binding to the hosts and early avoidance of late endosome-lysosome pathway...
- Neutrophil polarization: spatiotemporal dynamics of RhoA activity support a self-organizing mechanismKit Wong
Department of Cellular and Molecular Pharmacology, University of California, San Francisco, CA 94143 2140, USA
Proc Natl Acad Sci U S A 103:3639-44. 2006..Mutual incompatibility of frontness and backness is responsible for self-organization of neutrophil polarity...
- Cytotoxicity and apoptosis-inducing activity of bisphenol A and hydroquinone in HL-60 cellsHiroshi Terasaka
Department of Oral Diagnosis, Meikai University School of Dentistry, Saitama, Japan
Anticancer Res 25:2241-7. 2005..The cytotoxicity of BPA was 2-fold less than that of HQ, whereas the apoptosis-inducing activity of BPA was 10-fold less than that of HQ...
- The interactions of Anaplasma phagocytophilum, endothelial cells, and human neutrophilsMichael J Herron
Department of Entomology, University of Minnesota, St Paul, Minnesota 55108, USA
Ann N Y Acad Sci 1063:374-82. 2005....
- Multidrug resistance protein attenuates gemtuzumab ozogamicin-induced cytotoxicity in acute myeloid leukemia cellsRoland B Walter
Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109 1024, USA
Blood 102:1466-73. 2003..Because MK-571 and CSA failed to affect cytotoxicity in a portion of Pgp-positive/MRP-positive AML samples, additional resistance mechanisms are likely important...
- Gene expression-based high-throughput screening(GE-HTS) and application to leukemia differentiationKimberly Stegmaier
Department of Pediatric Oncology, Dana Farber Cancer Institute and Children s Hospital Boston, Harvard Medical School, Boston, Massachusetts 02115, USA
Nat Genet 36:257-63. 2004..The results indicate that GE-HTS may be a powerful, general approach for chemical screening...
- Chaetomugilins, new selectively cytotoxic metabolites, produced by a marine fish-derived Chaetomium speciesMuroga Yasuhide
Osaka University of Pharmaceutical Sciences, Takatsuki, Osaka, Japan
J Antibiot (Tokyo) 61:615-22. 2008..These compounds exhibited significant growth inhibition against cultured P388 cells and HL-60 cells. In addition, chaetomugilins A, C and F showed selective cytotoxic activities against 39 human cancer cell lines...
- AML-1, PU.1, and Sp3 regulate expression of human bactericidal/permeability-increasing proteinAndreas Lennartsson
Department of Hematology, Lund University, Lund, Sweden
Biochem Biophys Res Commun 311:853-63. 2003..In conclusion, we provide evidence for AML-1, PU.1, and Sp3 cooperatively and directly mediating BPI-expression during myeloid differentiation...
- Targeted antitumoral dehydrocrotonin nanoparticles with L-ascorbic acid 6-stearateLucas Frungillo
Laboratório de Cultura de Células e Biofármacos, Instituto de Biologia, Universidade Estadual de Campinas, UNICAMP, Campinas, SP, Brazil
J Pharm Sci 98:4796-807. 2009..In vitro toxicity assays showed that NP-AAS-DHC suspension was more effective as an antitumoral than free DHC or NP-DHC and increased apoptosis induction by receptor-mediated pathway...
- Differential expression of a C-terminal splice variant of phosphatidylinositol transfer protein beta lacking the constitutive-phosphorylated Ser262 that localizes to the Golgi compartmentClive P Morgan
Lipid Signalling Group, Department of Physiology, University College London, 21 University Street, Rockefeller Building, London WC1E 6JJ, UK
Biochem J 398:411-21. 2006..The presence of PITPbeta sp variants adds an extra level of proteome complexity and, in rat liver, the single gene for PITPbeta gives rise to seven distinct protein species that can be resolved on the basis of their charge differences...
- Biological activities and pore formation of Clostridium perfringens beta toxin in HL 60 cellsMasahiro Nagahama
Department of Microbiology, Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Yamashiro cho, Tokushima 770 8514, Japan
J Biol Chem 278:36934-41. 2003..We conclude that the toxin acts on HL 60 cells by binding to lipid rafts and forming a functional oligomer with 228 kDa.
- Effects of extracts of Coriolus versicolor (I'm-Yunity) on cell-cycle progression and expression of interleukins-1 beta,-6, and -8 in promyelocytic HL-60 leukemic cells and mitogenically stimulated and nonstimulated human lymphocytesTze Chen Hsieh
Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, NY, USA
J Altern Complement Med 8:591-602. 2002..Kowloon, Hong Kong) prepared from strain Cov-1 of the mushroom Coriolus versicolor...
- Effects of flow and diffusion on chemotaxis studies in a microfabricated gradient generatorGlenn M Walker
Vanderbilt Institute for Integrative Biosystems Research and Education, Vanderbilt University, Nashville, TN 37235, USA
Lab Chip 5:611-8. 2005....
- Differentiated HL-60 cells are a valid model system for the analysis of human neutrophil migration and chemotaxisAnna Barbara Hauert
Department of Pathology, University of Bern, Murtenstr 31, CH 3010 Bern, Switzerland
Int J Biochem Cell Biol 34:838-54. 2002..In summary, the results presented here show that differentiated HL-60 cells, stimulated with chemotactic peptide, are a valid model system to study molecular mechanisms of neutrophil emigration...
- Anaplasma phagocytophilum specifically induces tyrosine phosphorylation of ROCK1 during infectionVenetta Thomas
Section of Rheumatology, Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06520, USA
Cell Microbiol 9:1730-7. 2007..These data demonstrate a role for A. phagocytophilum-mediated ROCK1 phosphorylation in infection, and suggests that inhibiting this pathway may lead to new, non-antibiotic strategies to treat human granulocytic anaplasmosis...
- Sequential analysis of Anaplasma phagocytophilum msp2 transcription in murine and equine models of human granulocytic anaplasmosisDiana G Scorpio
Department of Molecular and Comparative Pathobiology, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
Clin Vaccine Immunol 15:418-24. 2008..Alteration in transcription of msp2 has no bearing on clinical disease in horses, suggesting the existence of a separate proinflammatory component differentially expressed with changing in vitro passage...
- Cell proliferation and CD11b expression are controlled independently during HL60 cell differentiation initiated by 1,25 alpha-dihydroxyvitamin D(3) or all-trans-retinoic acidM T Drayson
LRF Differentiation Programme, Division of Immunity and Infection, University of Birmingham, United Kingdom
Exp Cell Res 266:126-34. 2001..Differentiating agents must therefore regulate the proliferation and the maturation of differentiating myeloid cells by mechanisms that are at least partly independent...
- Mechanism of ADP ribosylation factor-stimulated phosphatidylinositol 4,5-bisphosphate synthesis in HL60 cellsAlison Skippen
Department of Physiology, University College London, London WC1E 6JJ, United Kingdom
J Biol Chem 277:5823-31. 2002..We therefore conclude that both PA derived from the PLD pathway and ARF proteins, by directly activating PIP 5-kinase, contribute to the regulation of PI(4,5)P(2) synthesis at the plasma membrane in HL60 cells...
- 5-Aza-2'-deoxycytidine induces p21WAF expression by demethylation of p73 leading to p53-independent apoptosis in myeloid leukemiaKarin Schmelz
Department of Hematology and Oncology, Universitatsmedizin Berlin, Charite, Campus Virchow, Berlin, Germany
Int J Cancer 114:683-95. 2005..We therefore conclude that p73 is a relevant target for methylation-dependent efficacy of 5-Aza-CdR in AML cells...
- Combination effects of alkylphosphocholines and gemcitabine in malignant and normal hematopoietic cellsMilka C Georgieva
Unit of Toxicology and Chemotherapy, German Cancer Research Center, 280 Im Neuenheimer Feld, 69120, Heidelberg, Germany
Cancer Lett 182:163-74. 2002..Thus, the combination of Gem and an alkylphosphocholine is advantageous in terms of their complementary mode of action, resulting in increased cytotoxicity and lowered myelotoxicity...
- Histone deimination as a response to inflammatory stimuli in neutrophilsIndira Neeli
Department of Molecular Sciences, University of Tennessee Health Science Center, Memphis, TN 38163, USA
J Immunol 180:1895-902. 2008..The possible association of a modified autoantigen with microbial components could, in predisposed individuals, increase the risk of autoimmunity...
- Dicoumarol impairs mitochondrial electron transport and pyrimidine biosynthesis in human myeloid leukemia HL-60 cellsDavid González-Aragón
Departamento de Biologia Celular, Fisiologia e Inmunologia, Facultad de Ciencias, Universidad de Cordoba, Campus Rabanales, Edificio Severo Ochoa, 3 a planta, 14014 Cordoba, Spain
Biochem Pharmacol 73:427-39. 2007..These actions must be taken into account when considering dicoumarol effects on cells...
- PTEN regulates motility but not directionality during leukocyte chemotaxisRosa Ana Lacalle
Department of Immunology and Oncology, Centro Nacional de Biotecnologia CSIC, UAM Campus de Cantoblanco, 28049 Madrid, Spain
J Cell Sci 117:6207-15. 2004..The results suggest a role for PTEN in regulating actin polymerization, but not directionality during mammalian cell chemotaxis...
- Inhibition by vitamin C of apoptosis induced by N-nitrosamines in HepG2 and HL-60 cellsNuria Arranz
Departamento de Nutricion, Bromatologia y Tecnologia de los Alimentos, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, Spain
J Appl Toxicol 28:788-96. 2008..Therefore, our findings suggest that inhibition of apoptosis may be one of the mechanisms by which vitamin C exerts its protective effect...
- The Leukemia-associated ETO homologues are differently expressed during hematopoietic differentiationSofia Rondin Lindberg
Department of Hematology, University of Lund, Lund, Sweden
Exp Hematol 33:189-98. 2005..In conclusion, the differential expression of the ETO homologues suggests that they may have a potential role in hematopoietic differentiation...
- The PCH family member proline-serine-threonine phosphatase-interacting protein 1 targets to the leukocyte uropod and regulates directed cell migrationKate M Cooper
Cellular and Molecular Biology Program, University of Wisconsin, Madison, WI 53706, USA
Mol Biol Cell 19:3180-91. 2008..Together, our findings demonstrate that PSTPIP1 is a novel component of the leukocyte uropod that regulates endocytosis and cell migration...
- Induction of S phase cell arrest and caspase activation by polysaccharide peptide isolated from Coriolus versicolor enhanced the cell cycle dependent activity and apoptotic cell death of doxorubicin and etoposide, but not cytarabine in HL-60 cellsKenrie Pui Yan Hui
Department of Zoology, The University of Hong Kong, Pokfulam Road, Hong Kong, SAR, P R China
Oncol Rep 14:145-55. 2005..Our data support the potential development of PSP as an adjuvant for leukemia treatment, but also imply the importance of understanding its interaction with individual anticancer agents...
- Synergistic antitumor effects of anthracenylmethyl homospermidine and alpha-difluoromethylornithine on promyelocytic leukemia HL60 cellsSong Qiang Xie
Institute of Natural Products and Medicinal Chemistry, Henan University, Kaifeng 475001, China
Toxicol In Vitro 22:352-8. 2008..All these findings demonstrated that the combination treatment with DFMO and ANTMHspd resulted in synergistic antitumor effects on HL60 cells...
- Proteomic analysis of plasma membrane lipid rafts of HL-60 cellsMitsuaki Yanagida
Institute for Environmental and Gender Specific Medicine, Juntendo University Graduate School of Medicine, Tomioka, Urayasu shi, Chiba, Japan
Proteomics 7:2398-409. 2007..Identified proteins, expression of which was altered by DMSO treatment, are expected to be candidate proteins involved in differentiation and functions of neutrophils...
- Analysis of neutrophil chemotaxisPaul A Nuzzi
Department of Molecular and Cellular Pharmacology, University of Wisconsin Medical School, Madison, WI, USA
Methods Mol Biol 370:23-36. 2007..We also discuss methods for HL-60 transfection using retroviral approaches and chemotaxis assays used with differentiated HL-60 cells...
- Induction of apoptosis in human leukaemic cells by IPENSpm, a novel polyamine analogue and anti-metaboliteAlison V Fraser
Departments of Medicine and Therapeutics and Biomedical Sciences, Polwarth Building, University of Aberdeen, Foresterhill, Aberdeen, AB25 2ZD, Scotland, U K
Biochem J 367:307-12. 2002..IPENSpm can be classified as a polyamine anti-metabolite and it may be a promising new lead compound in terms of treatment of some human cancers...
- Acteoside inhibits human promyelocytic HL-60 leukemia cell proliferation via inducing cell cycle arrest at G0/G1 phase and differentiation into monocyteKyung Won Lee
Department of Pharmaceutical Biochemistry, College of Pharmacy, Kyung Hee University, Hoegi dong, Seoul 130 701, Korea
Carcinogenesis 28:1928-36. 2007..These findings, for the first time, reveal the mechanism underlying the anti-proliferative effect of acteoside on human promyelocytic HL-60 cells...
- Lipid products of PI(3)Ks maintain persistent cell polarity and directed motility in neutrophilsFei Wang
Department of Cellular and Molecular Pharmacology and the Cardiovascular Research Institute, University of California, San Francisco, CA 94143 0450, USA
Nat Cell Biol 4:513-8. 2002..Thus reciprocal interplay between PtdIns(3,4,5)P(3) and polymerized actin initiates and maintains the asymmetry of intracellular signals responsible for cell polarity and directed motility...
- Prevention of UV-induced carcinogenesis by cyanidin-3-glucosideXianglin Shi; Fiscal Year: 2013..Present study attempts to identify cyanidin-3-glucoside, a plant-derived compound, as a mechanism-based preventive agent against UV-induced skin cancers. ..
- HTS for enhancers of neutrophil function that specifically augment PIP3 signalHongbo R Luo; Fiscal Year: 2012..Moreover, the identified activators can be directly utilized as starting chemical compounds for novel immune enhancer drug development, particularly for severe infections due to lack of enough pathogen killing capability. ..
- Iron in Mitochondrial Physiology and DiseasePaul A Lindahl; Fiscal Year: 2012..These iron complexes may also play a role in generating reactive oxygen species (ROS) which are thought to be responsible for some aspects of cellular damage and aging. ..
- Small Cell-Penetrating Glyco-Decoys Sidetrack Selectin Ligand BiosynthesisKhushi L Matta; Fiscal Year: 2013..This Phase I proposal meets the central tenet of the SBIR program for promising commercial products. ..
- CELLULAR UPTAKE AND INTERACTION OF IRON AND GALLIUMChristopher Chitambar; Fiscal Year: 1992..35S- methionine and 125I will be used to label and study the synthesis and release of the transferrin receptor from cells. It will be determined if the free transferrin receptor influences gallium cytotoxicity...
- PATHOGENESIS OF HUMAN GRANULOCYTIC EHRLICHIOSISUlrike Munderloh; Fiscal Year: 2001....
- FUNCTIONS OF RB PROTEASE(S) IN APOPTOSISQING DOU; Fiscal Year: 2000..These studies should provide insight into the molecular mechanisms of the ppRB degradation, and may provide a basis for the development of novel therapies aimed at activating the ppRB-PTS function in vivo. ..
- REGULATION OF ENZYME OF LIPID METABOLISMRobert Bell; Fiscal Year: 1990..Alterations of sphingolipid metabolites are likely of importance to the sphingolipidosis, progressive dementias and other neurological disorders, cancer, psoriasis, immune disorders and inflammation...
- NON-GENETIC CELL HETEROGENEITY IN TUMOR EVOLUTIONSui Huang; Fiscal Year: 2007..unreadable] [unreadable] [unreadable] [unreadable] [unreadable] [unreadable] [unreadable]..
- CELL DEATH INDUCED BY ANTICANCER AGENTSAlan Eastman; Fiscal Year: 2007..The ultimate goal of this research program will be to define which pathway and effector a specific leukemia uses, and then prescribe effective drug combinations that will be individualized for that patient. ..
- P-21 INDUCTION IN MYELOID DIFFERENTIATIONRichard Steinman; Fiscal Year: 1999..A clear understanding of p21 function in normal myelopoiesis will establish a context for determining if disruption of p21 function contributes to myelodysplastic syndrome or myeloid leukemias. ..
- MECHANISMS OF DRUG ACTION IN TUMOR CELL DIFFERENTIATIONMICHAEL MORIN; Fiscal Year: 1990....
- Role of Nuclear Transport in Acute LeukemiaNABEEL YASEEN; Fiscal Year: 2004..Looking at entire promoters will provide a global assessment of the effects of Nup98-HOXA9 on myeloid transcription. ..
- GLYCOSYLTRANSFERASE GENE EXPRESSION IN TRANSFORMED CELLSNevis Fregien; Fiscal Year: 1992..These probe will also be used to determine the molecular basis of the lesions in the mutant cells lines...
- High Throughput Profiling of Plant Extracts Using Engineered Heart TissuesTetsuro Wakatsuki; Fiscal Year: 2006..The ultimate goal of this project is to rapidly and cost-effectively identify an herbal remedy, or compounds from natural products, that could treat cardiovascular diseases. [unreadable] [unreadable] [unreadable]..
- GENETIC ELEMENTS CONTROLLING MYELOID DIFFERENTIATIONEdward Agura; Fiscal Year: 1993..These studies may contribute several important aspects to the understanding of normal and disordered eukaryotic gene regulation in myeloid lineages...
- REGULATION OF TUMOR PHENOTYPE BY EXTRACHROMOSOMAL DNAS Eckhardt; Fiscal Year: 1999..The data generated from these studies will be used to design novel therapeutic strategies for patients with cancer. ..
- PROTEIN KINASE C INHIBITORS AS CHEMOPREVENTIVE AGENTSRobert Bell; Fiscal Year: 1991..These studies should create new tools (drugs) which will facilitate acquisition of basic knowledge pertinent to testing the major conceptualizations developed. These drugs may rapidly translate into new chemopreventive agents...
- PH REGULATORY EXCHANGE KINETICS IN HL60 CELLSDiego Restrepo; Fiscal Year: 1993..The use of these techniques will allow a comprehensive study of intracellular pH regulation in a model system where individual exchanges are well characterized...
- FUNCTIONAL HETEROGENEITY OF PROTEIN KINASE C ISOFORMSMARIE WOOTEN; Fiscal Year: 1990..This study will provide information regarding differential regulation of PKC isoform expression and a means to examine the functional heterogeneity of this gene family...
- Apoptosis in Sepsis: Regulation by TNF & delta-PKCLaurie Kilpatrick; Fiscal Year: 2006..4: Assess the role of delta-PKC in regulating the assembly of TNFalpha anti-apoptotic signaling complexes and their association with the p60TNFR. ..
- OXIDATIVE EVENTS IN CANCER THERAPYLarry Oberley; Fiscal Year: 2005....
- NUCLEAR TRANSPORT IN THE GENESIS OF ACUTE LEUKEMIANABEEL YASEEN; Fiscal Year: 1999..The ultimate goal of this research is to determine how translocations involving 'can' lead to leukemia, and hence begin to understand the role of nuclear transport abnormalities in the causation of cancer. ..
- VISUALIZATION AND FUNCTION OF MITOCHONDRIAL FORMSThomas James; Fiscal Year: 1980..Work on how the mitochondrial mass changes over the cell cycle in HL60 cells is being carried out using flow-microfluorimetry. The fluorescent dye, rodamine 123, has proved to be highly specific in this study. ..
- CELLULAR SIGNALING OF THE CANNABINOID RECEPTORBegonia Ho; Fiscal Year: 1999..The goal is to test whether there is any specificity in receptor -> G protein -> effector coupling, by comparing the signaling mechanisms of two cannabinoid receptors in different cellular environment. ..
- Activation of the human neurtrophilHELEN KORCHAK; Fiscal Year: 2005..V Establish the role of d-PKC in downregulation of signalling pathways triggered by the p60TNFR. Determine if d-PKC acts on a serine residue(s) in the juxtamembrane region or the death domain. ..
- FLUORESCENCE ACTIVATED CELL SORTERAndrew Yen; Fiscal Year: 2004..The instrument requested is a Dako-Cytomation basic MoFlo plus a UV-vis, vis, and HeNe laser to provide independent UV, vis/488, and red excitation. ..
- Retinoic Acid Regulated Cell Differentiation: BLR1 RcptrAndrew Yen; Fiscal Year: 2005..It has the implication that the ultimate effects of RA can be governed by relevant growth factors signaling through their cognate receptors. ..
- Clinical application of multispectral imaging flow cytometryHans Minderman; Fiscal Year: 2008..The aim of the proposed studies is to determine necessary adjustments to this instrument's hardware and analysis software to accurately and efficiently use it in a clinical research setting. ..
- TOPOISOMERASES IN RETINOID INDUCED DIFFERENTIATIONAndrew Yen; Fiscal Year: 2003..Andrew Yen, who brings expertise on the molecular and cellular biology of retinoic acid-induced myeloid differentiation. ..
- Innate Effectors of rAls3p-N Anti-Candida VaccineBrad J Spellberg; Fiscal Year: 2010..PNS 398/2590 (Rev. 11/07) Page - Continuation Format Page ..
- Tyrosine phosphorylation during A. phagocytophilum invasionJacob Ijdo; Fiscal Year: 2008..unreadable] [unreadable] In the Unites States, Human Granulocytic Ehrlichiosis is an emerging tick-associated disease and in[unreadable] endemic areas the incidence is second only to Lyme disease. ..
- COPPER/ALBUMIN REDOX-CYCLING IN PREECLAMPSIAValerian Kagan; Fiscal Year: 2003....
- Irradiation Damage and Protection of Pulmonary Endothelium Oxidative LipidomicsValerian E Kagan; Fiscal Year: 2010....
- Adrenergic Antagonism During Murine CandidiasisBrad Spellberg; Fiscal Year: 2008..For these reasons, the potential for adrenergic antagonism to act as an effective immunotherapy is highly meritorious of investigation in this training application. ..
- ACOUSTICALLY ACTIVATED MICELLAR DRUG DELIVERYNatalya Rapoport; Fiscal Year: 2007..abstract_text> ..
- VITAMIN D PLANT ANTIOXIDANT SILIBININ IN LEUKEMIA TRANSLATIONAL STUDYGeorge P Studzinski; Fiscal Year: 2010..Thus, myeloid leukemia can be considered an orphan disease, and merits support from public sources for the development of novel therapy. ..
- IGG PLACENTAL TRANSPORT: ENDOTHELIAL CAVEOLAEJohn Robinson; Fiscal Year: 2004..Our approaches to testing the predictions of this hypothesis incorporate methods of cell biology, immunology, biochemistry, ultrastructural analysis, and molecular biology. ..
- Vaccine hurdle to anthrax and the emerging immune systemPranela Rameshwar; Fiscal Year: 2006..This study will also form the basis to develop in vivo model with human hematopoietic system to study anthrax infection and also to improve responses to anthrax vaccines. ..
- Characterization of A Novel 65kDa E-selectin Ligand on G-CSF Mobilized LeukocytesRobert Sackstein; Fiscal Year: 2008..It is anticipated that the results obtained will lead to new approaches for preventing or alleviating G-CSF-induced vascular and inflammatory complications [unreadable] [unreadable] [unreadable]..
- Microenvironmental mechanisms of leukemia cell survivalRichard Larson; Fiscal Year: 2009..In addition, we will share this data with Dr. Tom Look at the Dana Farber Cancer Center in order to correlate his genetic findings in his T-ALL Zebra fish model with our human studies. ..
- Microscale control of stem cell signaling using cell patterning and perfusionJoel Voldman; Fiscal Year: 2010..Our technology provides a new window into stem cell biology that will help determine new ways to control their behavior. ..
- Role of Polyamines in MYCN-Amplified NeuroblastomaANDRE BACHMANN; Fiscal Year: 2009..Our research will be useful in the development of novel treatments for the most aggressive forms of the childhood cancer neuroblastoma. ..
- Implications of Retinoid-Induced CD38 Antigen ExpressionKapil Mehta; Fiscal Year: 2006..unreadable] [unreadable]..
- G protein signal integration by multifunctional proteinsT Harden; Fiscal Year: 2006..abstract_text> ..
- AIRWAY SMOOTH MUSCLE IN HEALTH AND DISEASERONALD COBURN; Fiscal Year: 2004..In particular, all of these diseases cause physiological alterations that influence the length at which smooth muscle cells contract and relax. ..
- Chemoprevention of prostate cancer by resveratrolTze Chen Hsieh; Fiscal Year: 2005....
- BIOEFFECTS OF GAS BODY ACTIVATION IN MEDICAL ULTRASOUNDDouglas Miller; Fiscal Year: 2001..abstract_text> ..
- BREAST CANCER/BONE MARROW STROMAL INTERACTIONSPranela Rameshwar; Fiscal Year: 2003..Since BC metastasis involves two major cell subsets, mesenchymal (stroma) and epithelial (BC), aim 4 will determine if regulation of PPT-I, NK-1 and NK-2 is tissue specific by studying their promoters in these cell subsets. ..
- Structure & Biology of Hematopoietic E-/L-SelectinRobert Sackstein; Fiscal Year: 2006..This information will contribute greatly to our understanding of the adhesive systems that regulate steady-state and pathologic hematopoiesis, as well as hematopoietic recovery following stem cell transplantation. ..
- Identification of Anaplasma phagocytophilum adhesinsJASON CARLYON; Fiscal Year: 2006..Furthermore, it will identify novel targets for preventing and treating HGA and may pave the way for development of pharmacologic inhibitors of cellular adhesion events associated with disease. ..
- Actinobacillus actinomycetemcomitans Leukotoxin Induced Cell DeathEdward Lally; Fiscal Year: 2007..abstract_text> ..
- Preclinical Evaluation of Bcr/Abl-Directed TyrphostinsScott Kaufmann; Fiscal Year: 2007..abstract_text> ..
- Regulation of gp91phox by Anaplasma phagocytophilaVenetta Thomas; Fiscal Year: 2007..Aim 2. To define the effect of A. phagocytophila infection on the activity of the activator proteins and their binding capacity of the gp91phox promoter. ..
- Opiate's Effects on the Inflammation and Cytotoxicity in HIV-1 Transgenic RatSULIE CHANG; Fiscal Year: 2007..unreadable] [unreadable]..
- Isothiocyanates in the Chemoprevention of Bladder CancerYuesheng Zhang; Fiscal Year: 2007..Aim 5 will determine the efficacy of an orally administered ITC in inhibiting N-butyl-N- (4-hydroxybutyl)-nitrosamine-induced bladder tumorigenesis in F344 rats. ..
- Annual Midwest DNA Repair SymposiumAltaf Wani; Fiscal Year: 2007..unreadable] [unreadable] [unreadable] [unreadable]..
- NUCLEAR DIFFERENTATION DURING MURINE GRANULOPOIESISDONALD OLINS; Fiscal Year: 2004..abstract_text> ..
- Antisense Mediated Suppression of Dystrophin MutationsMichael Howard; Fiscal Year: 2007..unreadable] [unreadable]..
- PHOSPHATIDYLSERINE-SPECIFIC RECEPTOR FOR APOPTOTIC CELLSValerie Fadok; Fiscal Year: 2004..The investigators hypothesize that PS recognition has been preserved throughout phylogeny as an effective mechanism mediating the removal of apoantibodies. ..
- Role of Lamin B Receptor in Neutrophil Maturation and Functional ActivationPeter Gaines; Fiscal Year: 2007..The results may also offer important insight into the pathogenesis of potentially fatal hematologic disorders. [unreadable] [unreadable] [unreadable]..