Summary: The fundamental, structural, and functional units or subunits of living organisms. They are composed of CYTOPLASM containing various ORGANELLES and a CELL MEMBRANE boundary.

Top Publications

  1. pmc A simple salting out procedure for extracting DNA from human nucleated cells
    S A Miller
    Memorial Blood Center of Minneapolis, MN 55404
    Nucleic Acids Res 16:1215. 1988
  2. pmc Assessing mitochondrial dysfunction in cells
    Martin D Brand
    Buck Institute for Research on Aging, 8001 Redwood Blvd, Novato, CA 94945, USA
    Biochem J 435:297-312. 2011
  3. pmc Clearance of apoptotic cells: implications in health and disease
    Michael R Elliott
    Center for Cell Clearance and the Department of Microbiology, University of Virginia, Charlottesville, VA 22908, USA
    J Cell Biol 189:1059-70. 2010
  4. ncbi Microautophagy in mammalian cells: revisiting a 40-year-old conundrum
    Dalibor Mijaljica
    Department of Biochemistry and Molecular Biology, School of Biomedical Sciences, Monash University, Victoria, Australia
    Autophagy 7:673-82. 2011
  5. pmc Central dogma at the single-molecule level in living cells
    Gene Wei Li
    Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    Nature 475:308-15. 2011
  6. ncbi Nonsense-mediated mRNA decay in human cells: mechanistic insights, functions beyond quality control and the double-life of NMD factors
    Pamela Nicholson
    Institute of Cell Biology, University of Bern, Baltzerstrasse 4, 3012, Bern, Switzerland
    Cell Mol Life Sci 67:677-700. 2010
  7. ncbi Molecular identification of a danger signal that alerts the immune system to dying cells
    Yan Shi
    Department of Pathology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    Nature 425:516-21. 2003
  8. pmc From cells to organs: building polarized tissue
    David M Bryant
    Department of Anatomy, University of California San Francisco, California 94143 2140, USA
    Nat Rev Mol Cell Biol 9:887-901. 2008
  9. pmc A genetically encoded tag for correlated light and electron microscopy of intact cells, tissues, and organisms
    Xiaokun Shu
    Howard Hughes Medical Institute, University of California at San Diego, La Jolla, California, United States of America
    PLoS Biol 9:e1001041. 2011
  10. ncbi The regulation of extracellular signal-regulated kinase (ERK) in mammalian cells
    Joe W Ramos
    Department of Natural Products and Cancer Biology, Cancer Research Center of Hawaii, University of Hawaii at Manoa, 651 Ilalo Street, Honolulu, HI 96813, USA
    Int J Biochem Cell Biol 40:2707-19. 2008

Detail Information

Publications260 found, 100 shown here

  1. pmc A simple salting out procedure for extracting DNA from human nucleated cells
    S A Miller
    Memorial Blood Center of Minneapolis, MN 55404
    Nucleic Acids Res 16:1215. 1988
  2. pmc Assessing mitochondrial dysfunction in cells
    Martin D Brand
    Buck Institute for Research on Aging, 8001 Redwood Blvd, Novato, CA 94945, USA
    Biochem J 435:297-312. 2011
    ..Dysfunction can be assessed in isolated mitochondria, in cells or in vivo, with different balances between precise experimental control and physiological relevance...
  3. pmc Clearance of apoptotic cells: implications in health and disease
    Michael R Elliott
    Center for Cell Clearance and the Department of Microbiology, University of Virginia, Charlottesville, VA 22908, USA
    J Cell Biol 189:1059-70. 2010
    Recent advances in defining the molecular signaling pathways that regulate the phagocytosis of apoptotic cells have improved our understanding of this complex and evolutionarily conserved process...
  4. ncbi Microautophagy in mammalian cells: revisiting a 40-year-old conundrum
    Dalibor Mijaljica
    Department of Biochemistry and Molecular Biology, School of Biomedical Sciences, Monash University, Victoria, Australia
    Autophagy 7:673-82. 2011
    ..de Duve and Wattiaux and subsequently applied, over the following two decades, to processes described in mammalian cells and involving the presence of lysosome-like organelles having multiple vesicles trapped in their lumen ("..
  5. pmc Central dogma at the single-molecule level in living cells
    Gene Wei Li
    Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    Nature 475:308-15. 2011
    Gene expression originates from individual DNA molecules within living cells. Like many single-molecule processes, gene expression and regulation are stochastic, that is, sporadic in time...
  6. ncbi Nonsense-mediated mRNA decay in human cells: mechanistic insights, functions beyond quality control and the double-life of NMD factors
    Pamela Nicholson
    Institute of Cell Biology, University of Bern, Baltzerstrasse 4, 3012, Bern, Switzerland
    Cell Mol Life Sci 67:677-700. 2010
    ..In recent years, the discovery of additional functions played by several of the NMD factors has further complicated the picture. Therefore, we also review the reported roles of UPF1, SMG1 and SMG6 in other cellular processes...
  7. ncbi Molecular identification of a danger signal that alerts the immune system to dying cells
    Yan Shi
    Department of Pathology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    Nature 425:516-21. 2003
    ..Danger signals are thought to act by stimulating dendritic cells to mature so that they can present foreign antigens and stimulate T lymphocytes...
  8. pmc From cells to organs: building polarized tissue
    David M Bryant
    Department of Anatomy, University of California San Francisco, California 94143 2140, USA
    Nat Rev Mol Cell Biol 9:887-901. 2008
    How do animal cells assemble into tissues and organs? A diverse array of tissue structures and shapes can be formed by organizing groups of cells into different polarized arrangements and by coordinating their polarity in space and time...
  9. pmc A genetically encoded tag for correlated light and electron microscopy of intact cells, tissues, and organisms
    Xiaokun Shu
    Howard Hughes Medical Institute, University of California at San Diego, La Jolla, California, United States of America
    PLoS Biol 9:e1001041. 2011
    ..specific protein EM labeling has lacked generally applicable genetically encoded tags for in situ visualization in cells and tissues...
  10. ncbi The regulation of extracellular signal-regulated kinase (ERK) in mammalian cells
    Joe W Ramos
    Department of Natural Products and Cancer Biology, Cancer Research Center of Hawaii, University of Hawaii at Manoa, 651 Ilalo Street, Honolulu, HI 96813, USA
    Int J Biochem Cell Biol 40:2707-19. 2008
    ..Finally, several feedback mechanisms have been identified downstream of ERK activation. Here we will focus on the diverse mechanisms of ERK regulation in mammalian cells.
  11. pmc Reprogramming of murine and human somatic cells using a single polycistronic vector
    Bryce W Carey
    Whitehead Institute for Biomedical Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02142, USA
    Proc Natl Acad Sci U S A 106:157-62. 2009
    Directed reprogramming of somatic cells by defined factors provides a novel method for the generation of patient-specific stem cells with the potential to bypass both the practical and ethical concerns associated with somatic cell ..
  12. pmc Identification of genetic variants that affect histone modifications in human cells
    Graham McVicker
    Department of Human Genetics, University of Chicago, Chicago, IL 60637, USA
    Science 342:747-9. 2013
    ..Furthermore, variants that affect chromatin at distal regulatory sites frequently also direct changes in chromatin and gene expression at associated promoters. ..
  13. ncbi Electron tomography of cells
    Lu Gan
    Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA
    Q Rev Biophys 45:27-56. 2012
    ..to become the highest resolution three-dimensional (3D) imaging technique available for unique objects such as cells. Cells can be imaged either plastic-embedded or frozen-hydrated...
  14. ncbi Second-harmonic imaging microscopy for visualizing biomolecular arrays in cells, tissues and organisms
    Paul J Campagnola
    Center for Biomedical Imaging Technology, Department of Physiology, University of Connecticut Health Center, Farmington, Connecticut 06030, USA
    Nat Biotechnol 21:1356-60. 2003
    ..Better dyes and optimized microscope optics could ultimately lead to the imaging of neuronal electrical activity with SHIM...
  15. ncbi Direct effects of type I interferons on cells of the immune system
    Sandra Hervas-Stubbs
    Division of Gene Therapy and Hepatology, Centre for Applied Medical Research CIMA, University of Navarra, Pamplona, Spain
    Clin Cancer Res 17:2619-27. 2011
    ..induces the Janus activated kinase-signal transducer and activation of transcription (JAK-STAT) pathway in most cells, along with other biochemical pathways that may differentially operate, depending on the responding cell subset, ..
  16. ncbi Interaction of nanoparticles with cells
    Volker Mailänder
    Max Planck Institute for Polymer Research, Ackermannweg 10, 55128 Mainz, Germany
    Biomacromolecules 10:2379-400. 2009
    Nanoparticles and their interaction with human cells have been a focus of many groups during the past decade...
  17. ncbi There is no evidence that mitochondria are the main source of reactive oxygen species in mammalian cells
    Guy C Brown
    Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1QW, UK
    Mitochondrion 12:1-4. 2012
    It is often assumed that mitochondria are the main source of reactive oxygen species (ROS) in mammalian cells, but there is no convincing experimental evidence for this in the literature...
  18. ncbi Dynamics of single mRNP nucleocytoplasmic transport and export through the nuclear pore in living cells
    Amir Mor
    The Mina and Everard Goodman Faculty of Life Sciences and Institute of Nanotechnology, Bar Ilan University, Ramat Gan 52900, Israel
    Nat Cell Biol 12:543-52. 2010
    The flow of genetic information in eukaryotic cells occurs through the nucleocytoplasmic translocation of mRNAs. Knowledge of in vivo messenger RNA export kinetics remains poor in comparison with that of protein transport...
  19. ncbi Structural studies by electron tomography: from cells to molecules
    Vladan Lucic
    Department of Structural Biology, Max Planck Institute of Biochemistry, D 82152 Martinsried, Germany
    Annu Rev Biochem 74:833-65. 2005
    ..tomography (ET) is uniquely suited to obtain three-dimensional reconstructions of pleomorphic structures, such as cells, organelles or supramolecular assemblies...
  20. pmc Evidence for transcript networks composed of chimeric RNAs in human cells
    Sarah Djebali
    Bioinformatics and Genomics, Centre for Genomic Regulation and Universitat Pompeu Fabra, Barcelona, Catalonia, Spain
    PLoS ONE 7:e28213. 2012
    ..The non-random nature of the connection of the genes involved suggest that chimeric transcripts should not be studied in isolation, but together, as an RNA network...
  21. pmc Mobile DNA transposition in somatic cells
    Haig H Kazazian
    Institute for Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    BMC Biol 9:62. 2011
    ..germ-cell development rather than in somatic-cell development, but solid evidence for transposition in somatic cells is now accumulating...
  22. ncbi Artificial induction of autophagy around polystyrene beads in nonphagocytic cells
    Shouhei Kobayashi
    Kobe Advanced ICT Research Center, National Institute of Information and Communications Technology, Iwaoka cho, Nishi ku, Kobe, Japan
    Autophagy 6:36-45. 2010
    ..defense mechanism to kill invading bacteria such as group A Streptococcus in nonphagocytic epithelial-like cells. The cellular events underlying autophagosome formation upon bacterial invasion remain unclear due to the ..
  23. ncbi Compatibility of mammalian cells on surfaces of poly(dimethylsiloxane)
    Jessamine Ng Lee
    Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, Massachusetts 02138
    Langmuir 20:11684-91. 2004
    ..composition of poly(dimethylsiloxane) (PDMS) on the attachment and growth of several different types of mammalian cells: primary human umbilical artery endothelial cells (HUAECs), transformed 3T3 fibroblasts (3T3s), transformed ..
  24. ncbi Silver nanospheres are cytotoxic and genotoxic to fish cells
    John Pierce Wise
    Wise Laboratory of Environmental and Genetic Toxicology, University of Southern Maine, 96 Falmouth St, P O Box 9300, Portland, ME 04104, USA
    Aquat Toxicol 97:34-41. 2010
    ..1 and 0.3microg/cm(2) induced damage in 8, 10.8, 16 and 15.8% of metaphases and 10.8, 15.6, 24 and 24 total aberrations in 100 metaphases, respectively. These data show that silver nanoparticles are cytotoxic and genotoxic to fish cells.
  25. pmc Effector cells of both nonhemopoietic and hemopoietic origin are required for interferon (IFN)-gamma- and tumor necrosis factor (TNF)-alpha-dependent host resistance to the intracellular pathogen, Toxoplasma gondii
    G S Yap
    Immunobiology Section, Laboratory of Parasitic Diseases, National Institutes of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Exp Med 189:1083-92. 1999
    ..intracellular pathogens, it is unclear how they control the growth of microorganisms that reside in nonhemopoietic cells. Pathogens within such cells may be killed by metabolites secreted by activated macrophages or, alternatively, ..
  26. ncbi Oxidative protein folding in the secretory pathway and redox signaling across compartments and cells
    Eva Margittai
    Department of Medical Chemistry, Molecular Biology and Pathobiochemistry, Semmelweis University, Budapest, Hungary
    Traffic 12:1-8. 2011
  27. pmc Non-genetic heterogeneity of cells in development: more than just noise
    Sui Huang
    Institute for Biocomplexity and Informatics, Biological Sciences Bldg, Room 539D, University of Calgary, Calgary, AB T3A 0Y1, Canada
    Development 136:3853-62. 2009
    Cell-to-cell variability of gene expression in clonal populations of mammalian cells is ubiquitous...
  28. ncbi Primary and secondary mechanisms of action of visible to near-IR radiation on cells
    T Karu
    Laser Technology Research Center of Russian Academy of Sciences, Troitsk, Moscow Region, Russia
    J Photochem Photobiol B 49:1-17. 1999
    Cytochrome c oxidase is discussed as a possible photoacceptor when cells are irradiated with monochromatic red to near-IR radiation...
  29. pmc HITS-CLIP: panoramic views of protein-RNA regulation in living cells
    Robert B Darnell
    Laboratory of Neuro Oncology, The Rockefeller University, Howard Hughes Medical Institute, New York, NY 10065, USA
    Wiley Interdiscip Rev RNA 1:266-86. 2010
    The study of gene regulation in cells has recently begun to shift from a period dominated by the study of transcription factor-DNA interactions to a new focus on RNA regulation...
  30. ncbi Secretory protein trafficking and organelle dynamics in living cells
    J Lippincott-Schwartz
    Cell Biology and Metabolism Branch, NICHD, National Institutes of Health, Bethesda, Maryland 20892, USA
    Annu Rev Cell Dev Biol 16:557-89. 2000
    ..acting as reporters for protein localization and trafficking within the secretory membrane system of living cells have been used in a wide variety of applications, including time-lapse imaging, double-labeling, energy transfer, ..
  31. ncbi Chapter 9: Counting proteins in living cells by quantitative fluorescence microscopy with internal standards
    Jian Qiu Wu
    Department of Molecular Genetics, The Ohio State University, Columbus, Ohio 43210, USA
    Methods Cell Biol 89:253-73. 2008
    ..microscope can be treated as a spectrophotometer to measure the absolute number of fluorescent molecules in live cells (Wu and Pollard, 2005)...
  32. pmc Rewiring cells: synthetic biology as a tool to interrogate the organizational principles of living systems
    Caleb J Bashor
    Department of Cellular and Molecular Pharmacology, University of California, San Francisco, California 94158 2517, USA
    Annu Rev Biophys 39:515-37. 2010
  33. pmc Programmed necrosis induced by asbestos in human mesothelial cells causes high-mobility group box 1 protein release and resultant inflammation
    Haining Yang
    Cancer Research Center of Hawaii, John A Burns School of Medicine, University of Hawaii, Honolulu, HI 96813, USA
    Proc Natl Acad Sci U S A 107:12611-6. 2010
    ..has been linked to the release of cytokines and mutagenic reactive oxygen species (ROS) from inflammatory cells. Asbestos is cytotoxic to human mesothelial cells (HM), which appears counterintuitive for a carcinogen...
  34. pmc A method for producing transgenic cells using a multi-integrase system on a human artificial chromosome vector
    Shigeyuki Yamaguchi
    Department of Biomedical Science, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Sciences, Tottori University, Yonago, Japan
    PLoS ONE 6:e17267. 2011
    The production of cells capable of expressing gene(s) of interest is important for a variety of applications in biomedicine and biotechnology, including gene therapy and animal transgenesis...
  35. ncbi Engineered cobalt oxide nanoparticles readily enter cells
    Elena Papis
    Department of Biotechnology and Molecular Science, Insubria University, Varese, Italy
    Toxicol Lett 189:253-9. 2009
    ..To determine their potential toxicity to human cells, we measured cell viability and ROS formation in two human cell lines using CoCl2 for comparison...
  36. pmc Origin of first cells at terrestrial, anoxic geothermal fields
    Armen Y Mulkidjanian
    School of Physics, University of Osnabruck, D 49069 Osnabruck, Germany
    Proc Natl Acad Sci U S A 109:E821-30. 2012
    All cells contain much more potassium, phosphate, and transition metals than modern (or reconstructed primeval) oceans, lakes, or rivers...
  37. pmc Nanolitre liquid patterning in aqueous environments for spatially defined reagent delivery to mammalian cells
    H Tavana
    Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan 48109, USA
    Nat Mater 8:736-41. 2009
    ..These existing methods, therefore, are limited for patterning onto delicate surfaces of living cells because physical contact or substantially dry conditions are damaging to them...
  38. pmc Role of sulfatide in normal and pathological cells and tissues
    Tadanobu Takahashi
    Department of Biochemistry, School of Pharmaceutical Sciences, University of Shizuoka and Global COE Program for Innovation in Human Health Sciences, 52 1 Yada, Suruga ku, Shizuoka shi, Shizuoka 422 8526, Japan
    J Lipid Res 53:1437-50. 2012
  39. pmc On the evolution of cells
    Carl R Woese
    Department of Microbiology, University of Illinois at Urbana Champaign, 601 South Goodwin Avenue, B103 Chemical and Life Sciences Laboratory, Urbana, IL 61801 3709, USA
    Proc Natl Acad Sci U S A 99:8742-7. 2002
    ..Primitive cells did not carry a stable organismal genealogical trace. Primitive cellular evolution is basically communal...
  40. pmc Highly parallel genome-wide expression analysis of single mammalian cells
    Jian Bing Fan
    Research and Development, Illumina, Inc, San Diego, California, United States of America
    PLoS ONE 7:e30794. 2012
    ..We have developed a high-throughput amplification method for generating robust gene expression profiles using single cell or low RNA inputs...
  41. ncbi Pancreatic stellate cells: new target in the treatment of chronic pancreatitis
    Rupjyoti Talukdar
    Department of Gastroenterology, Pushpawati Singhania Research Institute, New Delhi, India
    J Gastroenterol Hepatol 23:34-41. 2008
    ..Recent in vitro and in vivo experiments have proven objectively the role of activated pancreatic stellate cells (PSC) in fibrogenesis in CP...
  42. ncbi Population dynamics in vasopressin cells
    Gareth Leng
    Centre for Integrative Physiology, University of Edinburgh, Edinburgh, UK
    Neuroendocrinology 88:160-72. 2008
    ..Vasopressin cells sense plasma osmolality and secrete appropriate levels of vasopressin from the neurohypophysis as needed to ..
  43. ncbi Death by protein damage in irradiated cells
    Michael J Daly
    Department of Pathology, Uniformed Services University of the Health Sciences USUHS, School of Medicine, Bethesda, MD 20814, USA
    DNA Repair (Amst) 11:12-21. 2012
    ..Mounting experimental evidence does not fit into this theoretical framework. Whereas DNA lesion-yields in cells exposed to a given dose and type of radiation appear to be fixed, protein lesion-yields are highly variable...
  44. ncbi Diverse apoptotic pathways in enterovirus 71-infected cells
    Shih Cheng Chang
    Graduate Institute of Basic Medical Sciences, Chang Gung University, Tao Yuan, Taiwan
    J Neurovirol 10:338-49. 2004
    ..This investigation conducts a comprehensive study of the apoptotic pathways in neural and non-neural cells following enterovirus 71 infection...
  45. pmc Auxin influx inhibitors 1-NOA, 2-NOA, and CHPAA interfere with membrane dynamics in tobacco cells
    Martina Lanková
    Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Rozvojova 263, CZ 165 02 Prague 6, Czech Republic
    J Exp Bot 61:3589-98. 2010
    ..The results suggest that these, previously unknown, activities of putative auxin influx inhibitors regulate overall auxin transport across the plasma membrane depending on the dynamics of particular membrane vesicles...
  46. ncbi Long noncoding RNAs in mammalian cells: what, where, and why?
    Ling Ling Chen
    Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, CT 06030, USA
    Wiley Interdiscip Rev RNA 1:2-21. 2010
    ..Here, we discuss some recent progress in our understanding of the functions of a number of important lncRNAs in mammalian cells.
  47. pmc Intracellular fluid flow in rapidly moving cells
    Kinneret Keren
    Department of Biochemistry and Howard Hughes Medical Institute, Stanford University School of Medicine, Stanford, California 94305, USA
    Nat Cell Biol 11:1219-24. 2009
    ..To investigate the existence and the direction of fluid flow in rapidly moving cells, we introduced inert quantum dots into the lamellipodia of fish epithelial keratocytes and analysed their ..
  48. ncbi The role of vinculin in the regulation of the mechanical properties of cells
    Claudia Tanja Mierke
    Center for Medical Physics and Technology, Biophysics Group, Friedrich Alexander University of Erlangen Nuremberg, Henkestrasse 91, Erlangen, 91052, Germany
    Cell Biochem Biophys 53:115-26. 2009
    ..an inhibitor of cell migration on planar substrates, because knock-out of vinculin in F9 mouse embryonic carcinoma cells and mouse embryonic fibroblasts showed increased cell motility on 2D substrates...
  49. ncbi Mechanisms of miRNA-mediated post-transcriptional regulation in animal cells
    Marina Chekulaeva
    Friedrich Miescher Institute for Biomedical Research, 4002 Basel, Switzerland
    Curr Opin Cell Biol 21:452-60. 2009
    ..In this report, we review current progress in understanding how miRNAs execute these effects in animals and we discuss some of the controversies regarding different modes of miRNA function...
  50. ncbi How can biochemical reactions within cells differ from those in test tubes?
    Allen P Minton
    Section on Physical Biochemistry, Laboratory of Biochemical Pharmacology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health U S DHHS, Bethesda, MD 20892, USA
    J Cell Sci 119:2863-9. 2006
    ..Greater than order-of-magnitude increases in association rate and equilibrium constants attributable to background interactions have been observed in simulated and actual intracellular environments...
  51. pmc Discovery of new cargo proteins that enter cells through clathrin-independent endocytosis
    Craig A Eyster
    Laboratory of Cell Biology, NHLBI, National Institutes of Health, Bethesda, Maryland 20892, USA
    Traffic 10:590-9. 2009
    ..lacking clathrin-targeting sequences, such as the major histocompatibility complex class I protein (MHCI), into cells. After internalization, vesicles containing MHCI fuse with transferrin-containing endosomes generated from ..
  52. pmc Microfluidic devices for measuring gene network dynamics in single cells
    Matthew R Bennett
    Department of Biochemistry and Cell Biology and Institute of Biosciences and Bioengineering, Rice University, 6100 Main Street, Houston, Texas 77005 1892, USA
    Nat Rev Genet 10:628-38. 2009
    ..Recent technological advances now make it possible to track the dynamics of gene networks in single cells under various environmental conditions using microfluidic 'lab-on-a-chip' devices, and researchers are using these ..
  53. pmc Physical limits of cells and proteomes
    Ken A Dill
    Laufer Center for Physical and Quantitative Biology and Department of Physics, Stony Brook University, New York, NY 11794, USA
    Proc Natl Acad Sci U S A 108:17876-82. 2011
    ..Why do mesophilic cells die around 50 °C? How can the maximal growth-rate temperature (around 37 °C) occur so close to the cell-..
  54. ncbi An ezrin-rich, rigid uropod-like structure directs movement of amoeboid blebbing cells
    Anna Lorentzen
    Institute of Cancer Research, Cancer Research UK, Cancer Research UK Tumour Cell Signalling Unit, 237 Fulham Road, London SW3 6JB, UK
    J Cell Sci 124:1256-67. 2011
    Melanoma cells can switch between an elongated mesenchymal-type and a rounded amoeboid-type migration mode. The rounded 'amoeboid' form of cell movement is driven by actomyosin contractility resulting in membrane blebbing...
  55. ncbi Splicing-independent recruitment of U1 snRNP to a transcription unit in living cells
    Béatrice Spiluttini
    Ecole Normale Superieure, Institut de Biologie de l ENS, IBENS, Paris, France
    J Cell Sci 123:2085-93. 2010
    ..The constitutive association of U1 small nuclear ribonucleoprotein (snRNP) with the transcription machinery might play a role in coupling transcription with pre-mRNA maturation...
  56. pmc How and where are nonsense mRNAs degraded in mammalian cells?
    Oliver Mühlemann
    Institute of Cell Biology, University of Bern, Bern, Switzerland
    RNA Biol 7:28-32. 2010
    ..Here we discuss recent evidence as to how and where mRNAs targeted to NMD are degraded in human cells. We discuss accumulating evidence that the decay step of human NMD can be initiated by two different mechanisms: ..
  57. pmc Shank-interacting protein-like 1 promotes tumorigenesis via PTEN inhibition in human tumor cells
    Lizhi He
    Division of Nephrology, Department of Medicine, McMaster University, Hamilton, Ontario, Canada
    J Clin Invest 120:2094-108. 2010
    ..shank-interacting protein-like 1 (SIPL1) as a PTEN-NR in human tumor cell lines and human primary cervical cancer cells. Ectopic SIPL1 expression protected human U87 glioma cells from PTEN-mediated growth inhibition and promoted the ..
  58. pmc Measuring enzyme activity in single cells
    Michelle L Kovarik
    Department of Chemistry, University of North Carolina, Chapel Hill, NC 27599, USA
    Trends Biotechnol 29:222-30. 2011
    Seemingly identical cells can differ in their biochemical state, function and fate, and this variability plays an increasingly recognized role in organism-level outcomes...
  59. ncbi Confocal/TEM overlay microscopy: a simple method for correlating confocal and electron microscopy of cells expressing GFP/YFP fusion proteins
    Douglas R Keene
    Research Department, Shriners Hospital for Children, Portland, OR 97239, USA
    Microsc Microanal 14:342-8. 2008
    ..of light microscopy and high resolution transmission electron microscopy (TEM) on components within identical cells has been problematic...
  60. pmc Correlative 3D imaging of whole mammalian cells with light and electron microscopy
    Gavin E Murphy
    Center for Cancer Research, National Cancer Institute, NIH, Bethesda, MD 20892, USA
    J Struct Biol 176:268-78. 2011
    ..We establish protocols that enable the routine generation of 3D image stacks of entire plastic-embedded mammalian cells by IA-SEM at resolutions of ∼10-20nm at high contrast and with minimal artifacts from the focused ion beam...
  61. ncbi Competition, collaboration and coordination--determining how cells bypass DNA damage
    Julian E Sale
    Medical Research Council Laboratory of Molecular Biology, Cambridge, UK
    J Cell Sci 125:1633-43. 2012
    b>Cells must overcome replication blocks that might otherwise lead to genomic instability or cell death...
  62. ncbi Stochastic protein expression in individual cells at the single molecule level
    Long Cai
    Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, Massachusetts 02138, USA
    Nature 440:358-62. 2006
    ..stochastic events of protein production are difficult to observe directly with measurements on large ensembles of cells owing to lack of synchronization among cells...
  63. ncbi FTIR microscopy of biological cells and tissue: data analysis using resonant Mie scattering (RMieS) EMSC algorithm
    Paul Bassan
    Manchester Interdisciplinary Biocentre, University of Manchester, 131 Princess Street, Manchester, M1 7DN, UK
    Analyst 137:1370-7. 2012
    Transmission and transflection infrared microscopy of biological cells and tissue suffer from significant baseline distortions due to scattering effects, predominantly resonant Mie scattering (RMieS)...
  64. ncbi Comparison of segmentation algorithms for fluorescence microscopy images of cells
    Alden A Dima
    Software and Systems Division, Information Technology Laboratory, National Institute of Standards and Technology, Gaithersburg, Maryland 20899, USA
    Cytometry A 79:545-59. 2011
    The analysis of fluorescence microscopy of cells often requires the determination of cell edges. This is typically done using segmentation techniques that separate the cell objects in an image from the surrounding background...
  65. ncbi Molecular and structural basis for N-glycan-dependent determination of glycoprotein fates in cells
    Yukiko Kamiya
    Okazaki Institute for Integrative Bioscience and Institute for Molecular Science, National Institutes of Natural Sciences, 5 1 Higashiyama, Myodaiji, Okazaki, Aichi 444 8787, Japan
    Biochim Biophys Acta 1820:1327-37. 2012
    ..e. folding in the endoplasmic reticulum (ER), vesicular transport between the ER and the Golgi complex, and ER-associated degradation of glycoproteins, by interacting with a panel of intracellular lectins in the early secretory pathway...
  66. pmc Sphingomyelin and sphingomyelin synthase (SMS) in the malignant transformation of glioma cells and in 2-hydroxyoleic acid therapy
    Gwendolyn Barcelo-Coblijn
    Laboratory of Molecular Cell Biomedicine, Department of Biology, University of the Balearic Islands, Carretera de Valldemossa Km 7 5, E 07122 Palma, Balearic Islands, Spain
    Proc Natl Acad Sci U S A 108:19569-74. 2011
    ..Here, we show that human cancer cells have markedly lower levels of sphingomyelin (SM) than nontumor (MRC-5) cells...
  67. ncbi Advances in tomography: probing the molecular architecture of cells
    Karen Fridman
    Department of Life Sciences and the National Institute for Biotechnology in the Negev, Ben Gurion University, Beer Sheva, 84105 Israel
    Nat Rev Mol Cell Biol 13:736-42. 2012
    Visualizing the dynamic molecular architecture of cells is instrumental for answering fundamental questions in cellular and structural biology...
  68. pmc CELLPEDIA: a repository for human cell information for cell studies and differentiation analyses
    Akiko Hatano
    Computational Biology Research Center, Advanced Industrial Science and Technology AIST, 2 4 7 Aomi, Koto ku, Tokyo 135 0064, Japan
    Database (Oxford) 2011:bar046. 2011
    CELLPEDIA is a repository database for current knowledge about human cells. It contains various types of information, such as cell morphologies, gene expression and literature references...
  69. ncbi New views of cells in 3D: an introduction to electron tomography
    Richard McIntosh
    Laboratory for 3D Electron Microscopy of Cells, Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, CO 80309 0347, USA
    Trends Cell Biol 15:43-51. 2005
    ..Electron tomography can provide such information about many kinds of cells and organelles.
  70. ncbi Glutamine breakdown in rapidly dividing cells: waste or investment?
    J Carlos Aledo
    Departamento de Biologia Molecular y Bioquimica, Facultad de Ciencias, Universidad de Malaga, 29071 Malaga, Spain
    Bioessays 26:778-85. 2004
    Tumours, and in general rapidly dividing cells, behave as dissipative devices that apparently waste glutamine, since its consumption seems to exceed both energetic and nitrogen needs...
  71. ncbi Anomalous transport in the crowded world of biological cells
    Felix Höfling
    Max Planck Institut für Intelligente Systeme, Heisenbergstraße 3, 70569 Stuttgart, and Institut für Theoretische Physik IV, Universitat Stuttgart, Pfaffenwaldring 57, 70569 Stuttgart, Germany
    Rep Prog Phys 76:046602. 2013
    ..This is commonly attributed to macromolecular crowding in the interior of cells and in cellular membranes, summarizing their densely packed and heterogeneous structures...
  72. ncbi Influence of the metabolic properties of human cells on the kinetic of formation of the major benzo[a]pyrene DNA adducts
    Caroline Marie
    Laboratoire des Lésions des Acides Nucléiques, LCIB UMR E 3 CEA UJF, DRFMC, CEA Grenoble, 17 Avenue des Martyrs 38054 Grenoble Cedex 9, France
    J Appl Toxicol 28:579-90. 2008
    ..of B[a]P exposure, the aim of the study was to identify the major stable DNA adducts in B[a]P-treated human cells. The role played by cellular metabolism on the nature and frequency of the DNA lesions was investigated using ..
  73. ncbi Rapid-on-chip determination of dielectric properties of biological cells using imaging techniques in a dielectrophoresis dot microsystem
    Henry O Fatoyinbo
    Centre for Biomedical Engineering, Faculty of Engineering and Physical Sciences, University of Surrey, Guildford, Surrey, UK
    Electrophoresis 29:3-10. 2008
    ..A specific application of this technique is deducing the dielectric properties of cells from analysis of the dielectrophoretic spectrum of that particular cell population...
  74. pmc The Cinderella syndrome: why do malaria-infected cells burst at midnight?
    Nicole Mideo
    Center for Infectious Disease Dynamics, The Pennsylvania State University, University Park, PA 16802, USA
    Trends Parasitol 29:10-6. 2013
  75. ncbi A novel method for the production of transgenic cloned pigs: electroporation-mediated gene transfer to non-cultured cells and subsequent selection with puromycin
    Satoshi Watanabe
    Department of Developmental Biology, Division of Insect and Animal, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, 305 0901, Japan
    Biol Reprod 72:309-15. 2005
    ..Somatic cells isolated from porcine fetuses at 73 days of gestation were immediately electroporated with a transgene (pCAG-..
  76. pmc Effects of ionizing radiation in nonirradiated cells
    William F Morgan
    Radiation Oncology Research Laboratory and Marlene and Stewart Greenebaum Cancer Center, University of Maryland, BRB 7 011, Baltimore, MD 21201 1509, USA
    Proc Natl Acad Sci U S A 102:14127-8. 2005
  77. pmc Quantification of PtdInsP3 molecular species in cells and tissues by mass spectrometry
    Jonathan Clark
    Inositide Laboratory, Babraham Institute, Babraham Research Campus, Cambridge, UK
    Nat Methods 8:267-72. 2011
    ..This methodology can be used to quantify multiple fatty-acyl species of PtdIns(3,4,5)P(3) in unstimulated mouse and human cells (≥10(5)) or tissues (≥0.1 mg) and their increase upon appropriate stimulation.
  78. ncbi Microfluidic devices for the high-throughput chemical analysis of cells
    Maxine A McClain
    Oak Ridge National Laboratory, P O Box 2008, Oak Ridge, Tennessee 37831 6142, USA
    Anal Chem 75:5646-55. 2003
    ..The device function was demonstrated using Jurkat cells that were loaded with the fluorogenic dyes - carboxyfluorescein diacetate, Oregon green carboxylic acid diacetate, ..
  79. ncbi Cell-based electrochemical biosensors for water quality assessment
    Florence Lagarde
    University of Lyon, Laboratoire des Sciences Analytiques, UMR, CNRS, CPE, Villeurbanne, France
    Anal Bioanal Chem 400:947-64. 2011
    ..b>Cells are easy to cultivate and manipulate, host many enzymes able to catalyse a wide range of biological reactions and ..
  80. pmc Computer-aided detection of centroblasts for follicular lymphoma grading using adaptive likelihood-based cell segmentation
    Olcay Sertel
    Department of Biomedical Informatics, The Ohio State University, Columbus, OH 43210, USA
    IEEE Trans Biomed Eng 57:2613-6. 2010
    ..for FL patients are based on histological grading, which is done by manually counting the large malignant cells called centroblasts (CB) in ten standard microscopic high-power fields from H&E-stained tissue sections...
  81. ncbi Nanosecond, high-intensity pulsed electric fields induce apoptosis in human cells
    Stephen J Beebe
    Center for Pediatric Research, 855 W Brambleton Ave, Norfolk, VA 23510, USA
    FASEB J 17:1493-5. 2003
    ..compared with the charging time of the plasma membrane can induce cell fusion or introduce xenomolecules into cells. Nanosecond pulse power technology generates pulses with high-intensity electric fields, but with such short ..
  82. ncbi Do we underestimate the importance of water in cell biology?
    Martin Chaplin
    Department of Applied Science, London South Bank University, Borough Road, London SE1 0AA, UK
    Nat Rev Mol Cell Biol 7:861-6. 2006
    ..Water can generate small active clusters and macroscopic assemblies, which can both transmit information on different scales...
  83. ncbi Treatment with insulin glargine (Lantus) increases the proliferative potency of the serum of patients with type-1 diabetes: a pilot study on MCF-7 breast cancer cells
    Doris Mayer
    Hormones and Signal Transduction Group, German Cancer Research Centre, DKFZ ZMBH Alliance, Im Neuenheimer Feld 581, Heidelberg, Germany
    Arch Physiol Biochem 116:73-8. 2010
    Insulin glargine (Lantus) stimulates growth of MCF-7 cells stronger than human insulin. We investigated if serum from diabetic patients treated with glargine versus human insulin may display a similar effect.
  84. pmc High-resolution nonlinear optical imaging of live cells by second harmonic generation
    P J Campagnola
    Department of Physiology and Center for Biomedical Imaging Technology, University of Connecticut Health Center, Farmington, Connecticut 06030 USA
    Biophys J 77:3341-9. 1999
    ..Second harmonic imaging (SHIM) is an ideal method for probing membranes of living cells because it offers the high resolution of nonlinear optical microscopy with the potential for near-total avoidance ..
  85. pmc HIV dynamics with multiple infections of target cells
    Narendra M Dixit
    Theoretical Biology and Biophysics, Theoretical Division, Los Alamos National Laboratory, Los Alamos, NM 87545, USA
    Proc Natl Acad Sci U S A 102:8198-203. 2005
    The high incidence of multiple infections of cells by HIV sets the stage for rapid HIV evolution by means of recombination. Yet how HIV dynamics proceeds with multiple infections remains poorly understood...
  86. ncbi Analysis of intracellular oxygen and metabolic responses of mammalian cells by time-resolved fluorometry
    Alexander V Zhdanov
    Luxcel Biosciences Ltd, BioInnovation Centre, UCC, Cork, Ireland
    Anal Chem 79:9414-9. 2007
    A simple, minimally invasive methodology for the analysis of intracellular oxygen in populations of live mammalian cells is described...
  87. pmc Modelling reaction kinetics inside cells
    Ramon Grima
    Institute for Mathematical Sciences, Imperial College, London, U K
    Essays Biochem 45:41-56. 2008
    ..molecule imaging techniques have given us a window into the intricate biochemical activities that occur inside cells. In this chapter we review four distinct theoretical and simulation frameworks: (i) non-spatial and deterministic, ..
  88. pmc Transcriptome and targetome analysis in MIR155 expressing cells using RNA-seq
    Guorong Xu
    Department of Computer Science, University of New Orleans, New Orleans, LA 70148, USA
    RNA 16:1610-22. 2010
  89. pmc Mechanisms and cellular roles of local protein synthesis in mammalian cells
    Alexis J Rodriguez
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, United States
    Curr Opin Cell Biol 20:144-9. 2008
    ..is mRNA subject to translation, but also through RNA-binding proteins and other trans-acting factors, eukaryotic cells interpret codes for spatial sorting within the mRNA sequence...
  90. pmc Interactions of Listeria monocytogenes with mammalian cells during entry and actin-based movement: bacterial factors, cellular ligands and signaling
    P Cossart
    Unité des Interactions Bactéries Cellules, Institut Pasteur, 28 rue du Docteur Roux, Paris 75015, France
    EMBO J 17:3797-806. 1998
    ..The mechanisms that Listeria uses to enter cells, escape from the phagocytic vacuole and spread from one cell to another using an actin-based motility process have ..
  91. pmc Noncontact measurement of the local mechanical properties of living cells using pressure applied via a pipette
    Daniel Sánchez
    Division of Medicine, Imperial College London, Hammersmith Hospital Campus, London, W12 0NN, United Kingdom
    Biophys J 95:3017-27. 2008
    ..to apply quantified positive and negative force at defined positions to the soft responsive surface of living cells. The method uses applied hydrostatic pressure (0...
  92. ncbi Biosensors based on the energy metabolism of living cells: the physical chemistry and cell biology of extracellular acidification
    J C Owicki
    Molecular Devices Corporation, Menlo Park, CA 94025
    Biosens Bioelectron 7:255-72. 1992
    ..is a biosensor-based instrument that detects changes in the physiological state of cultured living cells by monitoring the rate at which the cells excrete acidic products of metabolism...
  93. ncbi Optical techniques for imaging membrane lipid microdomains in living cells
    Dylan M Owen
    Chemical Biology Centre, Imperial College London, London, UK
    Semin Cell Dev Biol 18:591-8. 2007
    ..For example, trafficking and secretion of molecules can be targeted and directed, cells polarised and signalling events modulated and propagated...
  94. ncbi Probing nanomechanical properties from biomolecules to living cells
    S Kasas
    Laboratoire de Physique de la Matière Complexe, EPFL, CH 1015 Lausanne, Switzerland
    Pflugers Arch 456:13-27. 2008
    ..In this review, we will focus on the indentation technique and will illustrate its application to biological materials with examples that range from single molecules to living cells.
  95. ncbi Aneuploidy in mitosis of PtK1 cells is generated by random loss and nondisjunction of individual chromosomes
    Liliana Torosantucci
    IBPM Institute of Molecular Biology and Pathology, CNR National Research Council, University La Sapienza, Via degli Apuli 4, 00185 Rome, Italy
    J Cell Sci 122:3455-61. 2009
    ..of the six PtK1 chromosomes to undergo nondisjunction and/or chromosome loss was observed in ana-telophase cells recovering from a nocodazole- or a monastrol-induced mitotic arrest, suggesting that the recurrent presence of ..
  96. ncbi Weiss Lecture. Effects of radiations of different qualities on cells: molecular mechanisms of damage and repair
    D T Goodhead
    Medical Research Council Radiobiology Unit, Chilton, Didcot, UK
    Int J Radiat Biol 63:543-56. 1993
    ..of ionizing radiations of different quality are discussed with particular emphasis on damage to DNA of mammalian cells. Three related themes are followed...
  97. ncbi Defective interfering RNAs of Japanese encephalitis virus found in mosquito cells and correlation with persistent infection
    Kuen Nan Tsai
    Institute of Biotechnology, Department of Life Science, National Dong Hwa University, Taiwan, ROC
    Virus Res 124:139-50. 2007
    ..we have identified for the first time the generation of DI RNAs of Japanese encephalitis virus in C6/36 mosquito cells. A persistent infection was established by replacing growth medium on surviving cells and continued cell passaging...
  98. ncbi SCART scavenger receptors identify a novel subset of adult gammadelta T cells
    Jan Kisielow
    Molecular Biomedicine, Institute of Integrative Biology, Swiss Federal Institute of Technology ETH, Zurich Schlieren, Switzerland
    J Immunol 181:1710-6. 2008
    ..great progress in the characterization of alphabeta T cell differentiation, selection, and function, gammadelta T cells have remained poorly understood...
  99. pmc Paracrine signaling by glial cell-derived triiodothyronine activates neuronal gene expression in the rodent brain and human cells
    Beatriz C G Freitas
    Laboratory of Molecular Endocrinology, Division of Endocrinology, Department of Medicine, Federal University of Sao Paulo, Sao Paulo SP, Brazil
    J Clin Invest 120:2206-17. 2010
    ..Here, we modeled TH action in the brain using an in vitro coculture system of D2-expressing H4 human glioma cells and D3-expressing SK-N-AS human neuroblastoma cells...
  100. ncbi Measuring the elastic properties of living cells by the atomic force microscope
    Manfred Radmacher
    Drittes Physics Institute, Georg August Universitat, 37073 Gottingen, Germany
    Methods Cell Biol 68:67-90. 2002
    In this chapter I discussed the possibility of measuring elastic properties of living cells by AFM...
  101. ncbi Mechanism for action of electromagnetic fields on cells
    Dimitris J Panagopoulos
    Department of Cell Biology and Biophysics, Faculty of Biology, University of Athens, Panepistimiopolis, Athens GR 15784, Greece
    Biochem Biophys Res Commun 298:95-102. 2002
    A biophysical model for the action of oscillating electric fields on cells, presented by us before [Biochem. Biophys. Res. Commun...

Research Grants70

    BHANU JENA; Fiscal Year: 2003
    ..called 'pits' 8and 'depressions', have been identified and implicated in exocytosis in live pancreatic acinar cells. Our immediate research goal is to characterize and determine the involvement of these structures in the ..
    BHANU JENA; Fiscal Year: 2004
    ..Impairment of the exocytotic process in cells is involved in a number of disease states...
    BHANU JENA; Fiscal Year: 2001
    ..Impairment of the exocytotic process in cells is involved in a number of disease states...
    Michael S Kilberg; Fiscal Year: 2010
    ..We use amino acid deprivation of cultured cells to investigate transcriptional control by nutrients...
  5. Probing single proteins in nanofabricated bioenvironment
    Daniel Chiu; Fiscal Year: 2006
    ..We hypothesize that the rate and mechanisms of enzymatic actions and protein interactions in vivo inside cells differ significantly from the rate and mechanisms observed in vitro inside cuvettes or other macroscopic ..
  6. Nutritional Regulation of Ribosomal Protein Expression
    Michael Kilberg; Fiscal Year: 2005
    Mammalian cells respond to amino acid deprivation by increasing the transcription of a wide variety of genes by a pathway that will be referred to as the Amino Acid Response (AAR)...
  7. Cancer Risks Attributable To Radiation from Pediatric CT
    David Brenner; Fiscal Year: 2006
    ..The target audiences will be radiologists, pediatricians, family physicians, and the CT industry. We will use telephone interviews supplemented with survey questions, to aid in web content decisions. ..
  8. High Stability Accelerator for Sub-Cellular Microbeam
    David Brenner; Fiscal Year: 2003
    ..is the "front end" of our single particle I single cell microbeam which allows irradiation of individual cells and, increasingly, sub-cellular components...
  9. NMR detection of tumor differentiation
    EDWARD DELIKATNY; Fiscal Year: 2009
    ..PB), two differentiating agents currently under Phase II clinical trials, inhibit the proliferation of cancer cells. We will concentrate on the changes in phosphatidylcholine (PtdCho) metabolites that can be observed using in vivo ..
  10. Profiling individual synaptic vesicles with nanofluidics
    Daniel Chiu; Fiscal Year: 2003
  11. NMR and NIR detection of phospholipase activity in vivo
    EDWARD DELIKATNY; Fiscal Year: 2004
    ..with the differentiating agent phenylbutryate (PB) will lead to increased NIR fluorescence in the prostate tumor cells Aim 3): Demonstration of in vivo delivery and subsequent activation of phospholipases accompanying treatment of ..
  12. Profiling Biochemical Contents of Single Mitochondria
    Daniel Chiu; Fiscal Year: 2009
    ..for generating over 90% of the cell's ATP, are implicated in aging (the site of oxidative damage in cells), and are found to play a key role in apoptosis and calcium homeostasis...
  13. Single-Molecule Immunoassay and DNA Screening
    Edward Yeung; Fiscal Year: 2005
    We will develop novel imaging technologies for real-time comprehensive analysis of molecular alterations in cells and tissues appropriate for automation and adaptation to high-throughput applications...
    David Brenner; Fiscal Year: 2002
    ..abstract_text> ..
  15. Nutritional Control of Transcription Factor Expression
    Michael S Kilberg; Fiscal Year: 2010
    Mammalian cells respond to nutritional limitation of protein/amino acids by increasing the expression of a wide spectrum of proteins via a signaling pathway that will be referred to as the Amino Acid Response (AAR)...
  16. Iron and Vitamin E in Childhood HIV-Associated Renal Disease
    Patricio Ray; Fiscal Year: 2006
    ..The proposed studies should have a substantial impact on the goals of the National Center for Complementary and Alternative Medicine and NIH, according to the program announcement PA-02-124. [unreadable] [unreadable]..
  17. 2006 Single Molecule Approaches to Biology
    SUNNEY XIE; Fiscal Year: 2006
    ..unreadable] [unreadable] [unreadable]..
  18. Mitochondrial sites of reactive oxygen species generation in cells.
    Martin D Brand; Fiscal Year: 2010
    ..the rate of cellular mitochondrial ROS generation at each specific site in the electron transport chain in cells relevant to aging, particularly neurons, muscle cells and fibroblasts...
  19. Regulation of Cell Signaling and Adhesion
    JOE RAMOS; Fiscal Year: 2006
    ..In the PEA-15 null mouse, the most striking phenotype is a lymphoproliferative syndrome affecting both B and T cells. There is also a marked increase in hepatocyte proliferation and atrophy of the epidermis...
    Jean Schaffer; Fiscal Year: 2006
    ..Mismatch between LCFA import and utilization in the pancreas, skeletal muscle, vascular endothelial cells, and the heart may play an important role in the pathogenesis of diabetes and heart failure...
  21. Mitochondrial Role in Manganese Toxicity
    Thomas Gunter; Fiscal Year: 2010
    ..It is vital that we determine the causes for its neurotoxic effects. In the cells of its target brain tissue, primarily neurons of the globus pallidus and striatum, Mn is sequestered by the ..
  22. Ultrafast Electron Cryomicroscopy of Single Protein Particles
    Ahmed H Zewail; Fiscal Year: 2010
    ..If successful, this advance could make structure determination to near-atomic resolution routine for large macromolecular complexes. ..
    Arturo Casadevall; Fiscal Year: 2006
    ..These studies are expected to yield new insights on fundamental aspects of antibody function as well as practical information for the development of second generation antibody reagents for human therapy. ..
    Linda Griffith; Fiscal Year: 2007
    ..has been shown to influence cell growth and migration in a broad range of cell types (fibroblasts, epithelial cells, mesenchymal stem cells) and healing situations (skin wound repair, liver regeneration, angiogenesis) We ..
  25. Biopolymer Surfactants for Sealing Electroporated Membranes
    RAPHAEL CARL LEE; Fiscal Year: 2010
    ..Finally, we will conduct studies in parallel to determine if surfactant rescued cells manifest normal phenotype during proliferation or exhibit effects of mutations caused by unrepaired DMA.
  26. High Throughput Single Molecule DNA Sequencing
    Stephen Quake; Fiscal Year: 2007
    ..99%, while reducing the cost of sequencing a mammalian genome to below $100,000. [unreadable] [unreadable]..
  27. Therapeutic hair follicle-derived neurospheres
    Meng Yang; Fiscal Year: 2007
    unreadable] DESCRIPTION (provided by applicant): The facile availability of pluripotent adult stem cells would have many important therapeutic applications...
    Thomas McIntyre; Fiscal Year: 2008
    ..to the disease process because it creates inflammatory mediators that attract monocytes, and activates vascular cells and these infiltrating monocytes...
  29. Novel Chimeric Molecule to Improve Islet transplantation
    Ricardo Pastori; Fiscal Year: 2007
    ..Such approach could minimize necessity for multiple transplants thus reducing the problem due to the shortage of cadaveric donor pancreata available for islet transplantation. [unreadable] [unreadable] [unreadable]..
  30. DNA Sequencing using Nanopores
    Steven Benner; Fiscal Year: 2007
    ..These will then be targeted against specific sequences extracted from mammalian genomes. [unreadable] [unreadable]..
  31. Hepatitis HBx-Mediated Pathogenesis
    BETTY SLAGLE; Fiscal Year: 2007
    ..We additionally hypothesize that HBx holds nondividing cells at a G1 restriction point, and will use RNA inhibition to examine candidate proteins through which HBx may exert ..
  32. Polymerases for Sequencing by Synthesis
    Steven Benner; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  33. Prostate Cancer Immunotherapy
    Robert Darnell; Fiscal Year: 2007
    ..In both settings the outcomes are similar: most cells undergo growth arrest, but only few undergo apoptotic death...
  34. Realistic Phantom Series for OLINDA/EXM Version 2
    Michael Stabin; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  35. ECI Conference on Engineering Cell Biology II - The Cell in Context
    Linda Griffith; Fiscal Year: 2007
    ..How these principles define the architecture, organization, and function of cells and tissues in vivo will be compared to the effects of engineered substrates, biomaterials, and protein mimetics ..
  36. Endotoxin Generated Lipid Second Messengers
    Thomas McIntyre; Fiscal Year: 2006
    ..LPA stimulates PPRE-driven reporters, induces CD36 expression, and differentiates monocytes to foam cells. We show this signaling is independent of Edg (surface LPA receptors) signaling in several ways...
  37. Structure/function studies of RBPs in neurologic disease
    Robert Darnell; Fiscal Year: 2005
    ..the activity of these mutants using previously established assay systems in heterologous transfected neuroblastoma cells. From these experiments, we will choose "designer" mutants for in vivo expression in mouse brain...
    Stephen Goff; Fiscal Year: 2003
    ..In each case, these tests will be applied to existing panels of mutants to help localize the regions needed for these activities. ..
    Richard Zare; Fiscal Year: 2001
    ..abstract_text> ..
    FREDERICK MAXFIELD; Fiscal Year: 2004
    ..goal of this project is to understand how ligands, receptors, membrane lipids and solute molecules move through cells following endocytosis...
    THOMAS SQUIER; Fiscal Year: 2003
    ..age-related decreases in CaM function are responsible for the loss of calcium homeostasis observed in senescent cells. The accumulation of oxidatively modified CaM (CaMox) that is functionally inactive during aging is consistent ..
    Heping Zhang; Fiscal Year: 2003
  43. Complementation Assay for GPCR Desensitization
    RICHARD EGLEN; Fiscal Year: 2003
    ..Furthermore, these assays could have a broader role in drug discovery by identifying agonists at the large population of orphan GPCRs since stimulation of all GPCRs causes BARK and B-arrestin translocation. ..
    Eileen White; Fiscal Year: 2001
    ..The long term goal of the work is to study this important mechanism of tumor suppression. ..
    Byron Goldstein; Fiscal Year: 2002
    ..The studies in this project are health related, bearing on allergic reactions and their treatment as well as other aspects of the immune response. ..
    Michael Daly; Fiscal Year: 2001
    ..These studies will delineate the genetics and biochemistry of radioresistance in D. radiodurans. Insight into its strategies may have broad implications for understanding radioresistance and DSB-metabolism throughout nature. ..
    MYRTLE DAVIS; Fiscal Year: 2001
    ..p53, bcl-2, c- jun/AP-1 and cell cycle inhibitory proteins in okadaic acid-induced apoptosis of renal epithelial cells. We have found that transformation of renal epithelial cells with ras-oncogene increases sensitivity of renal ..
  48. Signal Transduction by Cytokine Receptors in Vivo
    Warner Greene; Fiscal Year: 2004
    ..These proteins trigger similar biochemical effects within their respective target cells through receptors that have structural and functional similarities with one another...
  49. Calcium ATPases in Apicomplexan Parasites as Potential D
    L Sibley; Fiscal Year: 2006
    ..Apicomplexan parasites rely on active invasion to enter host cells and this obligatory step is controlled in part by the regulated secretion of adhesins from micronemes...
  50. Trafficking of Channels Measured by EFC
    RICHARD EGLEN; Fiscal Year: 2004
    ..These initial studies will form the foundation of future phase II SBIR studies to develop EFC into a HTS assay for ICC transport for use by bioteclmology and pharmaceutical companies for drug discovery. ..
  51. Mining slime mold for susceptibility genes to fungi
    Arturo Casadevall; Fiscal Year: 2005
    ..Our group has shown that C. neoformans can infect D. discoideum, replicate intracellularly, and kill the amoeboid cells. D...
  52. Targeted Assisted Combinational Synthesis
    Steven Benner; Fiscal Year: 2004
    ..abstract_text> ..
  53. Tolerance versus tumor immunity in PND
    Robert Darnell; Fiscal Year: 2005
    ..These experiments offer the possibility of developing new cancer treatments and advancing the understanding of autoimmune disease of the nervous system. ..
  54. Comparative Morphology of Neuronal Ceroid Lipofuscinosis
    Jonathan Cooper; Fiscal Year: 2004
    ..abstract_text> ..
    Shan Yu; Fiscal Year: 2002
    ..Apoptotic cells undergo marked cell volume decrease...
  56. Dementia of Parkinson Type: Clinicopathologic Phenotype
    James Galvin; Fiscal Year: 2004
    ..At the completion of the award period, candidate plans to develop an independent R01 project derived from the data generated by the experiments described in this proposal. ..
    SUNNEY XIE; Fiscal Year: 2004
    ..Scattering (CARS) microscopy to the next level: imaging of single macromolecules, biological membranes, and living cells using vibrational spectroscopy. The specific aims of the proposed studies are: 1...
  58. Leukocyte Responses to Endotoxin
    Thomas McIntyre; Fiscal Year: 2005
    ..We anticipate this work will lead to a new class of anti-inflammatory agents. ..
  59. Dynamic Interactions of the Cytoskeleton
    ERIKA HOLZBAUR; Fiscal Year: 2008
    ..We have recently localized cytoplasmic dynein to the cortex of mammalian epithelial cells during interphase, where it is specifically enriched at sites of cell-cell contact...
  60. CaP, crystal responsible for idiopathic nephrolithasis
    Saeed Khan; Fiscal Year: 2008
    ..Type density and size must conform to limits and specifications provided in the PHS 398Instructions. RESEARCH GRANT TABLE OF CONTENTS Page Numbers Face Page 1 Description, ..
    Saeed Khan; Fiscal Year: 2003
    ..Significantly high level of oxalate however, injures the renal epithelial cells. The injury results in promotion of crystal formation and their retention within the renal tubules by providing ..
  62. Probabilistic Modeling of Information from Images and Text in Online Journals
    Robert Murphy; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  63. Centriole Orientation During Left/Right Symmetry Breaking in the Mouse
    Wallace Marshall; Fiscal Year: 2007
    ..This nodal flow is created, and possibly sensed, by cilia on the node cells. How do the cilia know which way to drive the fluid? Theoretical computations as well as the investigators' ..
  64. Regulation of Cell Signaling and Adhesion
    JOE RAMOS; Fiscal Year: 2007
    ..The hypothesis is that PEA-15 regulates the decision of T lymphocytes and some cancer cells to proliferate or to die...
  65. Determining Subcellular Drug Distributions
    Edgar Arriaga; Fiscal Year: 2004
    ..It is hypothesized that the subcellular localization of this drug in a few drug-treated single cells will accurately represent drug localization in the original cell culture...
  66. International Workshops on Iron and Copper Homeostasis
    Michael Garrick; Fiscal Year: 2004
    ..The organizers face a demand for a continuation and series. Clearly one can expect the third - fifth conferences to extend the success of the previous two to more scientists and trainees and more interacting metals ..
  67. Small Molecule Inhibitors of Cilia
    Wallace Marshall; Fiscal Year: 2007
    ..Because the cells are green, this causes a dark green pellet to self-organize in the well...
  68. MSM Multiscale Modeling of the Heart in Cardiovascular
    DANIEL BEARD; Fiscal Year: 2007
    ..abstract_text> ..