Genomes and Genes
Summary: The fundamental, structural, and functional units or subunits of living organisms. They are composed of CYTOPLASM containing various ORGANELLES and a CELL MEMBRANE boundary.
Publications235 found, 100 shown here
- Assessing mitochondrial dysfunction in cellsMartin D Brand
Buck Institute for Research on Aging, 8001 Redwood Blvd, Novato, CA 94945, USA
Biochem J 435:297-312. 2011..Dysfunction can be assessed in isolated mitochondria, in cells or in vivo, with different balances between precise experimental control and physiological relevance...
- A simple salting out procedure for extracting DNA from human nucleated cellsS A Miller
Memorial Blood Center of Minneapolis, MN 55404
Nucleic Acids Res 16:1215. 1988
- Molecular identification of a danger signal that alerts the immune system to dying cellsYan Shi
Department of Pathology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
Nature 425:516-21. 2003..Danger signals are thought to act by stimulating dendritic cells to mature so that they can present foreign antigens and stimulate T lymphocytes...
- Microautophagy in mammalian cells: revisiting a 40-year-old conundrumDalibor Mijaljica
Department of Biochemistry and Molecular Biology, School of Biomedical Sciences, Monash University, Victoria, Australia
Autophagy 7:673-82. 2011..de Duve and Wattiaux and subsequently applied, over the following two decades, to processes described in mammalian cells and involving the presence of lysosome-like organelles having multiple vesicles trapped in their lumen ("..
- Central dogma at the single-molecule level in living cellsGene Wei Li
Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, USA
Nature 475:308-15. 2011Gene expression originates from individual DNA molecules within living cells. Like many single-molecule processes, gene expression and regulation are stochastic, that is, sporadic in time...
- Nonsense-mediated mRNA decay in human cells: mechanistic insights, functions beyond quality control and the double-life of NMD factorsPamela Nicholson
Institute of Cell Biology, University of Bern, Baltzerstrasse 4, 3012, Bern, Switzerland
Cell Mol Life Sci 67:677-700. 2010..In recent years, the discovery of additional functions played by several of the NMD factors has further complicated the picture. Therefore, we also review the reported roles of UPF1, SMG1 and SMG6 in other cellular processes...
- HITS-CLIP: panoramic views of protein-RNA regulation in living cellsRobert B Darnell
Laboratory of Neuro Oncology, The Rockefeller University, Howard Hughes Medical Institute, New York, NY 10065, USA
Wiley Interdiscip Rev RNA 1:266-86. 2010The study of gene regulation in cells has recently begun to shift from a period dominated by the study of transcription factor-DNA interactions to a new focus on RNA regulation...
- A genetically encoded tag for correlated light and electron microscopy of intact cells, tissues, and organismsXiaokun Shu
Howard Hughes Medical Institute, University of California at San Diego, La Jolla, California, United States of America
PLoS Biol 9:e1001041. 2011..specific protein EM labeling has lacked generally applicable genetically encoded tags for in situ visualization in cells and tissues...
- Clearance of apoptotic cells: implications in health and diseaseMichael R Elliott
Center for Cell Clearance and the Department of Microbiology, University of Virginia, Charlottesville, VA 22908, USA
J Cell Biol 189:1059-70. 2010Recent advances in defining the molecular signaling pathways that regulate the phagocytosis of apoptotic cells have improved our understanding of this complex and evolutionarily conserved process...
- Reprogramming of murine and human somatic cells using a single polycistronic vectorBryce W Carey
Whitehead Institute for Biomedical Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02142, USA
Proc Natl Acad Sci U S A 106:157-62. 2009Directed reprogramming of somatic cells by defined factors provides a novel method for the generation of patient-specific stem cells with the potential to bypass both the practical and ethical concerns associated with somatic cell ..
- Direct effects of type I interferons on cells of the immune systemSandra Hervas-Stubbs
Division of Gene Therapy and Hepatology, Centre for Applied Medical Research CIMA, University of Navarra, Pamplona, Spain
Clin Cancer Res 17:2619-27. 2011..induces the Janus activated kinase-signal transducer and activation of transcription (JAK-STAT) pathway in most cells, along with other biochemical pathways that may differentially operate, depending on the responding cell subset, ..
- Interaction of nanoparticles with cellsVolker Mailänder
Max Planck Institute for Polymer Research, Ackermannweg 10, 55128 Mainz, Germany
Biomacromolecules 10:2379-400. 2009Nanoparticles and their interaction with human cells have been a focus of many groups during the past decade...
- The regulation of extracellular signal-regulated kinase (ERK) in mammalian cellsJoe W Ramos
Department of Natural Products and Cancer Biology, Cancer Research Center of Hawaii, University of Hawaii at Manoa, 651 Ilalo Street, Honolulu, HI 96813, USA
Int J Biochem Cell Biol 40:2707-19. 2008..Finally, several feedback mechanisms have been identified downstream of ERK activation. Here we will focus on the diverse mechanisms of ERK regulation in mammalian cells.
- Evidence for transcript networks composed of chimeric RNAs in human cellsSarah Djebali
Bioinformatics and Genomics, Centre for Genomic Regulation and Universitat Pompeu Fabra, Barcelona, Catalonia, Spain
PLoS ONE 7:e28213. 2012..The non-random nature of the connection of the genes involved suggest that chimeric transcripts should not be studied in isolation, but together, as an RNA network...
- On the evolution of cellsCarl R Woese
Department of Microbiology, University of Illinois at Urbana Champaign, 601 South Goodwin Avenue, B103 Chemical and Life Sciences Laboratory, Urbana, IL 61801 3709, USA
Proc Natl Acad Sci U S A 99:8742-7. 2002..Primitive cells did not carry a stable organismal genealogical trace. Primitive cellular evolution is basically communal...
- Mechanisms of miRNA-mediated post-transcriptional regulation in animal cellsMarina Chekulaeva
Friedrich Miescher Institute for Biomedical Research, 4002 Basel, Switzerland
Curr Opin Cell Biol 21:452-60. 2009..In this report, we review current progress in understanding how miRNAs execute these effects in animals and we discuss some of the controversies regarding different modes of miRNA function...
- Physical limits of cells and proteomesKen A Dill
Laufer Center for Physical and Quantitative Biology and Department of Physics, Stony Brook University, New York, NY 11794, USA
Proc Natl Acad Sci U S A 108:17876-82. 2011..Why do mesophilic cells die around 50 °C? How can the maximal growth-rate temperature (around 37 °C) occur so close to the cell-..
- Silver nanospheres are cytotoxic and genotoxic to fish cellsJohn Pierce Wise
Wise Laboratory of Environmental and Genetic Toxicology, University of Southern Maine, 96 Falmouth St, P O Box 9300, Portland, ME 04104, USA
Aquat Toxicol 97:34-41. 2010..1 and 0.3microg/cm(2) induced damage in 8, 10.8, 16 and 15.8% of metaphases and 10.8, 15.6, 24 and 24 total aberrations in 100 metaphases, respectively. These data show that silver nanoparticles are cytotoxic and genotoxic to fish cells.
- Effector cells of both nonhemopoietic and hemopoietic origin are required for interferon (IFN)-gamma- and tumor necrosis factor (TNF)-alpha-dependent host resistance to the intracellular pathogen, Toxoplasma gondiiG S Yap
Immunobiology Section, Laboratory of Parasitic Diseases, National Institutes of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
J Exp Med 189:1083-92. 1999..intracellular pathogens, it is unclear how they control the growth of microorganisms that reside in nonhemopoietic cells. Pathogens within such cells may be killed by metabolites secreted by activated macrophages or, alternatively, ..
- Oxidative protein folding in the secretory pathway and redox signaling across compartments and cellsEva Margittai
Department of Medical Chemistry, Molecular Biology and Pathobiochemistry, Semmelweis University, Budapest, Hungary
Traffic 12:1-8. 2011....
- Second-harmonic imaging microscopy for visualizing biomolecular arrays in cells, tissues and organismsPaul J Campagnola
Center for Biomedical Imaging Technology, Department of Physiology, University of Connecticut Health Center, Farmington, Connecticut 06030, USA
Nat Biotechnol 21:1356-60. 2003..Better dyes and optimized microscope optics could ultimately lead to the imaging of neuronal electrical activity with SHIM...
- Non-genetic heterogeneity of cells in development: more than just noiseSui Huang
Institute for Biocomplexity and Informatics, Biological Sciences Bldg, Room 539D, University of Calgary, Calgary, AB T3A 0Y1, Canada
Development 136:3853-62. 2009Cell-to-cell variability of gene expression in clonal populations of mammalian cells is ubiquitous...
- Dynamics of single mRNP nucleocytoplasmic transport and export through the nuclear pore in living cellsAmir Mor
The Mina and Everard Goodman Faculty of Life Sciences and Institute of Nanotechnology, Bar Ilan University, Ramat Gan 52900, Israel
Nat Cell Biol 12:543-52. 2010The flow of genetic information in eukaryotic cells occurs through the nucleocytoplasmic translocation of mRNAs. Knowledge of in vivo messenger RNA export kinetics remains poor in comparison with that of protein transport...
- Population dynamics in vasopressin cellsGareth Leng
Centre for Integrative Physiology, University of Edinburgh, Edinburgh, UK
Neuroendocrinology 88:160-72. 2008..Vasopressin cells sense plasma osmolality and secrete appropriate levels of vasopressin from the neurohypophysis as needed to ..
- Pancreatic stellate cells: new target in the treatment of chronic pancreatitisRupjyoti Talukdar
Department of Gastroenterology, Pushpawati Singhania Research Institute, New Delhi, India
J Gastroenterol Hepatol 23:34-41. 2008..Recent in vitro and in vivo experiments have proven objectively the role of activated pancreatic stellate cells (PSC) in fibrogenesis in CP...
- Diverse apoptotic pathways in enterovirus 71-infected cellsShih Cheng Chang
Graduate Institute of Basic Medical Sciences, Chang Gung University, Tao Yuan, Taiwan
J Neurovirol 10:338-49. 2004..This investigation conducts a comprehensive study of the apoptotic pathways in neural and non-neural cells following enterovirus 71 infection...
- Death by protein damage in irradiated cellsMichael J Daly
Department of Pathology, Uniformed Services University of the Health Sciences USUHS, School of Medicine, Bethesda, MD 20814, USA
DNA Repair (Amst) 11:12-21. 2012..Mounting experimental evidence does not fit into this theoretical framework. Whereas DNA lesion-yields in cells exposed to a given dose and type of radiation appear to be fixed, protein lesion-yields are highly variable...
- Intracellular fluid flow in rapidly moving cellsKinneret Keren
Department of Biochemistry and Howard Hughes Medical Institute, Stanford University School of Medicine, Stanford, California 94305, USA
Nat Cell Biol 11:1219-24. 2009..To investigate the existence and the direction of fluid flow in rapidly moving cells, we introduced inert quantum dots into the lamellipodia of fish epithelial keratocytes and analysed their ..
- Auxin influx inhibitors 1-NOA, 2-NOA, and CHPAA interfere with membrane dynamics in tobacco cellsMartina Lanková
Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Rozvojova 263, CZ 165 02 Prague 6, Czech Republic
J Exp Bot 61:3589-98. 2010..The results suggest that these, previously unknown, activities of putative auxin influx inhibitors regulate overall auxin transport across the plasma membrane depending on the dynamics of particular membrane vesicles...
- Long noncoding RNAs in mammalian cells: what, where, and why?Ling Ling Chen
Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, CT 06030, USA
Wiley Interdiscip Rev RNA 1:2-21. 2010..Here, we discuss some recent progress in our understanding of the functions of a number of important lncRNAs in mammalian cells.
- The role of vinculin in the regulation of the mechanical properties of cellsClaudia Tanja Mierke
Center for Medical Physics and Technology, Biophysics Group, Friedrich Alexander University of Erlangen Nuremberg, Henkestrasse 91, Erlangen, 91052, Germany
Cell Biochem Biophys 53:115-26. 2009..an inhibitor of cell migration on planar substrates, because knock-out of vinculin in F9 mouse embryonic carcinoma cells and mouse embryonic fibroblasts showed increased cell motility on 2D substrates...
- Stochastic protein expression in individual cells at the single molecule levelLong Cai
Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, Massachusetts 02138, USA
Nature 440:358-62. 2006..stochastic events of protein production are difficult to observe directly with measurements on large ensembles of cells owing to lack of synchronization among cells...
- Splicing-independent recruitment of U1 snRNP to a transcription unit in living cellsBéatrice Spiluttini
Ecole Normale Superieure, Institut de Biologie de l ENS, IBENS, Paris, France
J Cell Sci 123:2085-93. 2010..The constitutive association of U1 small nuclear ribonucleoprotein (snRNP) with the transcription machinery might play a role in coupling transcription with pre-mRNA maturation...
- How and where are nonsense mRNAs degraded in mammalian cells?Oliver Mühlemann
Institute of Cell Biology, University of Bern, Bern, Switzerland
RNA Biol 7:28-32. 2010..Here we discuss recent evidence as to how and where mRNAs targeted to NMD are degraded in human cells. We discuss accumulating evidence that the decay step of human NMD can be initiated by two different mechanisms: ..
- Origin of first cells at terrestrial, anoxic geothermal fieldsArmen Y Mulkidjanian
School of Physics, University of Osnabruck, D 49069 Osnabruck, Germany
Proc Natl Acad Sci U S A 109:E821-30. 2012All cells contain much more potassium, phosphate, and transition metals than modern (or reconstructed primeval) oceans, lakes, or rivers...
- Effects of ionizing radiation in nonirradiated cellsWilliam F Morgan
Radiation Oncology Research Laboratory and Marlene and Stewart Greenebaum Cancer Center, University of Maryland, BRB 7 011, Baltimore, MD 21201 1509, USA
Proc Natl Acad Sci U S A 102:14127-8. 2005
- Computer-aided detection of centroblasts for follicular lymphoma grading using adaptive likelihood-based cell segmentationOlcay Sertel
Department of Biomedical Informatics, The Ohio State University, Columbus, OH 43210, USA
IEEE Trans Biomed Eng 57:2613-6. 2010..for FL patients are based on histological grading, which is done by manually counting the large malignant cells called centroblasts (CB) in ten standard microscopic high-power fields from H&E-stained tissue sections...
- Engineered cobalt oxide nanoparticles readily enter cellsElena Papis
Department of Biotechnology and Molecular Science, Insubria University, Varese, Italy
Toxicol Lett 189:253-9. 2009..To determine their potential toxicity to human cells, we measured cell viability and ROS formation in two human cell lines using CoCl2 for comparison...
- Artificial induction of autophagy around polystyrene beads in nonphagocytic cellsShouhei Kobayashi
Kobe Advanced ICT Research Center, National Institute of Information and Communications Technology, Iwaoka cho, Nishi ku, Kobe, Japan
Autophagy 6:36-45. 2010..defense mechanism to kill invading bacteria such as group A Streptococcus in nonphagocytic epithelial-like cells. The cellular events underlying autophagosome formation upon bacterial invasion remain unclear due to the ..
- An ezrin-rich, rigid uropod-like structure directs movement of amoeboid blebbing cellsAnna Lorentzen
Institute of Cancer Research, Cancer Research UK, Cancer Research UK Tumour Cell Signalling Unit, 237 Fulham Road, London SW3 6JB, UK
J Cell Sci 124:1256-67. 2011Melanoma cells can switch between an elongated mesenchymal-type and a rounded amoeboid-type migration mode. The rounded 'amoeboid' form of cell movement is driven by actomyosin contractility resulting in membrane blebbing...
- Highly parallel genome-wide expression analysis of single mammalian cellsJian Bing Fan
Research and Development, Illumina, Inc, San Diego, California, United States of America
PLoS ONE 7:e30794. 2012..We have developed a high-throughput amplification method for generating robust gene expression profiles using single cell or low RNA inputs...
- Compatibility of mammalian cells on surfaces of poly(dimethylsiloxane)Jessamine Ng Lee
Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, Massachusetts 02138
Langmuir 20:11684-91. 2004..composition of poly(dimethylsiloxane) (PDMS) on the attachment and growth of several different types of mammalian cells: primary human umbilical artery endothelial cells (HUAECs), transformed 3T3 fibroblasts (3T3s), transformed ..
- Cell-based electrochemical biosensors for water quality assessmentFlorence Lagarde
University of Lyon, Laboratoire des Sciences Analytiques, UMR, CNRS, CPE, Villeurbanne, France
Anal Bioanal Chem 400:947-64. 2011..b>Cells are easy to cultivate and manipulate, host many enzymes able to catalyse a wide range of biological reactions and ..
- Quantification of PtdInsP3 molecular species in cells and tissues by mass spectrometryJonathan Clark
Inositide Laboratory, Babraham Institute, Babraham Research Campus, Cambridge, UK
Nat Methods 8:267-72. 2011..This methodology can be used to quantify multiple fatty-acyl species of PtdIns(3,4,5)P(3) in unstimulated mouse and human cells (≥10(5)) or tissues (≥0.1 mg) and their increase upon appropriate stimulation.
- A novel method for the production of transgenic cloned pigs: electroporation-mediated gene transfer to non-cultured cells and subsequent selection with puromycinSatoshi Watanabe
Department of Developmental Biology, Division of Insect and Animal, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, 305 0901, Japan
Biol Reprod 72:309-15. 2005..Somatic cells isolated from porcine fetuses at 73 days of gestation were immediately electroporated with a transgene (pCAG-..
- Microfluidic devices for the high-throughput chemical analysis of cellsMaxine A McClain
Oak Ridge National Laboratory, P O Box 2008, Oak Ridge, Tennessee 37831 6142, USA
Anal Chem 75:5646-55. 2003..The device function was demonstrated using Jurkat cells that were loaded with the fluorogenic dyes - carboxyfluorescein diacetate, Oregon green carboxylic acid diacetate, ..
- There is no evidence that mitochondria are the main source of reactive oxygen species in mammalian cellsGuy C Brown
Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1QW, UK
Mitochondrion 12:1-4. 2012It is often assumed that mitochondria are the main source of reactive oxygen species (ROS) in mammalian cells, but there is no convincing experimental evidence for this in the literature...
- Treatment with insulin glargine (Lantus) increases the proliferative potency of the serum of patients with type-1 diabetes: a pilot study on MCF-7 breast cancer cellsDoris Mayer
Hormones and Signal Transduction Group, German Cancer Research Centre, DKFZ ZMBH Alliance, Im Neuenheimer Feld 581, Heidelberg, Germany
Arch Physiol Biochem 116:73-8. 2010Insulin glargine (Lantus) stimulates growth of MCF-7 cells stronger than human insulin. We investigated if serum from diabetic patients treated with glargine versus human insulin may display a similar effect.
- Do we underestimate the importance of water in cell biology?Martin Chaplin
Department of Applied Science, London South Bank University, Borough Road, London SE1 0AA, UK
Nat Rev Mol Cell Biol 7:861-6. 2006..Water can generate small active clusters and macroscopic assemblies, which can both transmit information on different scales...
- High-resolution nonlinear optical imaging of live cells by second harmonic generationP J Campagnola
Department of Physiology and Center for Biomedical Imaging Technology, University of Connecticut Health Center, Farmington, Connecticut 06030 USA
Biophys J 77:3341-9. 1999..Second harmonic imaging (SHIM) is an ideal method for probing membranes of living cells because it offers the high resolution of nonlinear optical microscopy with the potential for near-total avoidance ..
- Transcriptome and targetome analysis in MIR155 expressing cells using RNA-seqGuorong Xu
Department of Computer Science, University of New Orleans, New Orleans, LA 70148, USA
RNA 16:1610-22. 2010....
- Discovery of new cargo proteins that enter cells through clathrin-independent endocytosisCraig A Eyster
Laboratory of Cell Biology, NHLBI, National Institutes of Health, Bethesda, Maryland 20892, USA
Traffic 10:590-9. 2009..lacking clathrin-targeting sequences, such as the major histocompatibility complex class I protein (MHCI), into cells. After internalization, vesicles containing MHCI fuse with transferrin-containing endosomes generated from ..
- Modelling reaction kinetics inside cellsRamon Grima
Institute for Mathematical Sciences, Imperial College, London, U K
Essays Biochem 45:41-56. 2008..molecule imaging techniques have given us a window into the intricate biochemical activities that occur inside cells. In this chapter we review four distinct theoretical and simulation frameworks: (i) non-spatial and deterministic, ..
- Analysis of intracellular oxygen and metabolic responses of mammalian cells by time-resolved fluorometryAlexander V Zhdanov
Luxcel Biosciences Ltd, BioInnovation Centre, UCC, Cork, Ireland
Anal Chem 79:9414-9. 2007A simple, minimally invasive methodology for the analysis of intracellular oxygen in populations of live mammalian cells is described...
- HIV dynamics with multiple infections of target cellsNarendra M Dixit
Theoretical Biology and Biophysics, Theoretical Division, Los Alamos National Laboratory, Los Alamos, NM 87545, USA
Proc Natl Acad Sci U S A 102:8198-203. 2005The high incidence of multiple infections of cells by HIV sets the stage for rapid HIV evolution by means of recombination. Yet how HIV dynamics proceeds with multiple infections remains poorly understood...
- Mechanisms and cellular roles of local protein synthesis in mammalian cellsAlexis J Rodriguez
Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, United States
Curr Opin Cell Biol 20:144-9. 2008..is mRNA subject to translation, but also through RNA-binding proteins and other trans-acting factors, eukaryotic cells interpret codes for spatial sorting within the mRNA sequence...
- Role of sulfatide in normal and pathological cells and tissuesTadanobu Takahashi
Department of Biochemistry, School of Pharmaceutical Sciences, University of Shizuoka and Global COE Program for Innovation in Human Health Sciences, 52 1 Yada, Suruga ku, Shizuoka shi, Shizuoka 422 8526, Japan
J Lipid Res 53:1437-50. 2012....
- Biosensors based on the energy metabolism of living cells: the physical chemistry and cell biology of extracellular acidificationJ C Owicki
Molecular Devices Corporation, Menlo Park, CA 94025
Biosens Bioelectron 7:255-72. 1992..is a biosensor-based instrument that detects changes in the physiological state of cultured living cells by monitoring the rate at which the cells excrete acidic products of metabolism...
- Noncontact measurement of the local mechanical properties of living cells using pressure applied via a pipetteDaniel Sanchez
Division of Medicine, Imperial College London, Hammersmith Hospital Campus, London, W12 0NN, United Kingdom
Biophys J 95:3017-27. 2008..to apply quantified positive and negative force at defined positions to the soft responsive surface of living cells. The method uses applied hydrostatic pressure (0...
- Interactions of Listeria monocytogenes with mammalian cells during entry and actin-based movement: bacterial factors, cellular ligands and signalingP Cossart
Unité des Interactions Bactéries Cellules, Institut Pasteur, 28 rue du Docteur Roux, Paris 75015, France
EMBO J 17:3797-806. 1998..The mechanisms that Listeria uses to enter cells, escape from the phagocytic vacuole and spread from one cell to another using an actin-based motility process have ..
- Shank-interacting protein-like 1 promotes tumorigenesis via PTEN inhibition in human tumor cellsLizhi He
Division of Nephrology, Department of Medicine, McMaster University, Hamilton, Ontario, Canada
J Clin Invest 120:2094-108. 2010..shank-interacting protein-like 1 (SIPL1) as a PTEN-NR in human tumor cell lines and human primary cervical cancer cells. Ectopic SIPL1 expression protected human U87 glioma cells from PTEN-mediated growth inhibition and promoted the ..
- Sphingomyelin and sphingomyelin synthase (SMS) in the malignant transformation of glioma cells and in 2-hydroxyoleic acid therapyGwendolyn Barcelo-Coblijn
Laboratory of Molecular Cell Biomedicine, Department of Biology, University of the Balearic Islands, Carretera de Valldemossa Km 7 5, E 07122 Palma, Balearic Islands, Spain
Proc Natl Acad Sci U S A 108:19569-74. 2011..Here, we show that human cancer cells have markedly lower levels of sphingomyelin (SM) than nontumor (MRC-5) cells...
- Optical techniques for imaging membrane lipid microdomains in living cellsDylan M Owen
Chemical Biology Centre, Imperial College London, London, UK
Semin Cell Dev Biol 18:591-8. 2007..For example, trafficking and secretion of molecules can be targeted and directed, cells polarised and signalling events modulated and propagated...
- Influence of the metabolic properties of human cells on the kinetic of formation of the major benzo[a]pyrene DNA adductsCaroline Marie
Laboratoire des Lésions des Acides Nucléiques, LCIB UMR E 3 CEA UJF, DRFMC, CEA Grenoble, 17 Avenue des Martyrs 38054 Grenoble Cedex 9, France
J Appl Toxicol 28:579-90. 2008..of B[a]P exposure, the aim of the study was to identify the major stable DNA adducts in B[a]P-treated human cells. The role played by cellular metabolism on the nature and frequency of the DNA lesions was investigated using ..
- Probing nanomechanical properties from biomolecules to living cellsS Kasas
Laboratoire de Physique de la Matière Complexe, EPFL, CH 1015 Lausanne, Switzerland
Pflugers Arch 456:13-27. 2008..In this review, we will focus on the indentation technique and will illustrate its application to biological materials with examples that range from single molecules to living cells.
- Weiss Lecture. Effects of radiations of different qualities on cells: molecular mechanisms of damage and repairD T Goodhead
Medical Research Council Radiobiology Unit, Chilton, Didcot, UK
Int J Radiat Biol 63:543-56. 1993..of ionizing radiations of different quality are discussed with particular emphasis on damage to DNA of mammalian cells. Three related themes are followed...
- Aneuploidy in mitosis of PtK1 cells is generated by random loss and nondisjunction of individual chromosomesLiliana Torosantucci
IBPM Institute of Molecular Biology and Pathology, CNR National Research Council, University La Sapienza, Via degli Apuli 4, 00185 Rome, Italy
J Cell Sci 122:3455-61. 2009..of the six PtK1 chromosomes to undergo nondisjunction and/or chromosome loss was observed in ana-telophase cells recovering from a nocodazole- or a monastrol-induced mitotic arrest, suggesting that the recurrent presence of ..
- Programmed necrosis induced by asbestos in human mesothelial cells causes high-mobility group box 1 protein release and resultant inflammationHaining Yang
Cancer Research Center of Hawaii, John A Burns School of Medicine, University of Hawaii, Honolulu, HI 96813, USA
Proc Natl Acad Sci U S A 107:12611-6. 2010..has been linked to the release of cytokines and mutagenic reactive oxygen species (ROS) from inflammatory cells. Asbestos is cytotoxic to human mesothelial cells (HM), which appears counterintuitive for a carcinogen...
- Microfluidic devices for measuring gene network dynamics in single cellsMatthew R Bennett
Department of Biochemistry and Cell Biology and Institute of Biosciences and Bioengineering, Rice University, 6100 Main Street, Houston, Texas 77005 1892, USA
Nat Rev Genet 10:628-38. 2009..Recent technological advances now make it possible to track the dynamics of gene networks in single cells under various environmental conditions using microfluidic 'lab-on-a-chip' devices, and researchers are using these ..
- Defective interfering RNAs of Japanese encephalitis virus found in mosquito cells and correlation with persistent infectionKuen Nan Tsai
Institute of Biotechnology, Department of Life Science, National Dong Hwa University, Taiwan, ROC
Virus Res 124:139-50. 2007..we have identified for the first time the generation of DI RNAs of Japanese encephalitis virus in C6/36 mosquito cells. A persistent infection was established by replacing growth medium on surviving cells and continued cell passaging...
- Measuring the elastic properties of living cells by the atomic force microscopeManfred Radmacher
Drittes Physics Institute, Georg August Universitat, 37073 Gottingen, Germany
Methods Cell Biol 68:67-90. 2002In this chapter I discussed the possibility of measuring elastic properties of living cells by AFM...
- SCART scavenger receptors identify a novel subset of adult gammadelta T cellsJan Kisielow
Molecular Biomedicine, Institute of Integrative Biology, Swiss Federal Institute of Technology ETH, Zurich Schlieren, Switzerland
J Immunol 181:1710-6. 2008..great progress in the characterization of alphabeta T cell differentiation, selection, and function, gammadelta T cells have remained poorly understood...
- Mechanism for action of electromagnetic fields on cellsDimitris J Panagopoulos
Department of Cell Biology and Biophysics, Faculty of Biology, University of Athens, Panepistimiopolis, Athens GR 15784, Greece
Biochem Biophys Res Commun 298:95-102. 2002A biophysical model for the action of oscillating electric fields on cells, presented by us before [Biochem. Biophys. Res. Commun...
- Paracrine signaling by glial cell-derived triiodothyronine activates neuronal gene expression in the rodent brain and human cellsBeatriz C G Freitas
Laboratory of Molecular Endocrinology, Division of Endocrinology, Department of Medicine, Federal University of Sao Paulo, Sao Paulo SP, Brazil
J Clin Invest 120:2206-17. 2010..Here, we modeled TH action in the brain using an in vitro coculture system of D2-expressing H4 human glioma cells and D3-expressing SK-N-AS human neuroblastoma cells...
- Cell encapsulating droplet vitrificationUtkan Demirci
Harvard Massachusetts Institute of Technology Health Sciences and Technology, Cambridge, MA, USA
Lab Chip 7:1428-33. 2007The capability to encapsulate single cells in droplets while retaining high cell viability (>90%) has great impact on tissue engineering, high-throughput screening, as well as clinical diagnostics and therapeutics...
- X-cells in fish pseudotumors are parasitic protozoansSatoshi Miwa
Inland Station, National Research Institute of Aquaculture, Tamaki, Mie 519 0423, Japan
Dis Aquat Organ 58:165-70. 2004..These lesions usually contain masses of so called 'X-cells' of unknown origin...
- Delivery of proteins and peptides into live cells by means of protein transduction domains: potential application to organ and cell transplantationRicardo L Pastori
Diabetes Research Institute, University of Miami School of Medicine, Miami, FL 33136, USA
Transplantation 77:1627-31. 2004
- Impact of image segmentation on high-content screening data quality for SK-BR-3 cellsAndrew A Hill
Department of Biological Technologies, Wyeth Research, 35 CambridgePark Drive, Cambridge, MA 02140, USA
BMC Bioinformatics 8:340. 2007..HCS uses automated microscopy to collect images of cultured cells. The images are subjected to segmentation algorithms to identify cellular structures and quantitate their ..
- Meganuclease-driven targeted integration in CHO-K1 cells for the fast generation of HTS-compatible cell-based assaysJean Pierre Cabaniols
Cellectis BioResearch, Romainville Cedex, France
J Biomol Screen 15:956-67. 2010..The authors developed a targeted integration system in Chinese hamster ovary (CHO) cells, called the cellular genome positioning system (cGPS), based on the stimulation of homologous gene targeting by ..
- Synthetic double-stranded RNA oligonucleotides are immunostimulatory for chicken spleen cellsA I Villanueva
Department of Pathobiology, University of Guelph, Guelph, Ontario, Canada N1G 2W1
Dev Comp Immunol 35:28-34. 2011..Although certain synthetic dsRNA molecules have been identified to show immunostimulation in mammalian cells, very little is known about the ability of these sequences to stimulate avian cells...
- Dynamic organization of chromatin assembly and transcription factories in living cellsBidisha Sinha
National Centre for Biological Sciences, Tata Institute of Fundamental Research, Bellary Road, Bangalore 560065, India
Methods Cell Biol 98:57-78. 2010..proteins, nuclear bodies and chromatin assembly are found to be highly dynamic within the nucleus of primary cells and through cellular differentiation programs...
- Time scales of water dynamics at biological interfaces: peptides, proteins and cellsJohan Qvist
Center for Molecular Protein Science, Department of Biophysical Chemistry, Lund University, SE 22100 Lund, Sweden
Faraday Discuss 141:131-44; discussion 175-207. 2009..is used to study water dynamics in the hydration layers of two small peptides, two globular proteins and in living cells of two microorganisms...
- The effect of inflammation on the generation of plasma DNA from dead and dying cells in the peritoneumNing Jiang
Division of Rheumatology, Duke University Medical Center, Box 151G, 508 Fulton St, Durham, NC 27705, USA
J Leukoc Biol 77:296-302. 2005To assess the effects of inflammation on the generation of circulating DNA from dead and dying cells, plasma DNA levels were determined in BALB/c mice, administered apoptotic or necrotic Jurkat cells following induction of peritonitis by ..
- Speciation of manganese in cells and mitochondria: a search for the proximal cause of manganese neurotoxicityThomas E Gunter
Department of Biophysics and Biochemistry, University of Rochester School of Medicine and Dentistry, 575 Elmwood Avenue, Rochester, NY 14642, USA
Neurotoxicology 27:765-76. 2006Recent studies of speciation of manganese (Mn) in brain mitochondria, neuron-like cells, and astrocytes are reviewed. No evidence is found for oxidation of Mn(2+) complexes to a Mn(3+) complex...
- ATR (ataxia telangiectasia mutated- and Rad3-related kinase) is activated by mild hypothermia in mammalian cells and subsequently activates p53Anne Roobol
Centre for Molecular Processing and Protein Science Group, School of Biosciences, University of Kent, Canterbury, Kent CT2 7NJ, UK
Biochem J 435:499-508. 2011In vitro cultured mammalian cells respond to mild hypothermia (27-33 °C) by attenuating cellular processes and slowing and arresting the cell cycle...
- Micropatterning as a tool to decipher cell morphogenesis and functionsManuel Thery
Laboratoire de Physiologie Cellulaire et Végétale, iRTSV, CEA CNRS UJF INRA, 17 rue des Martyrs, 38054, Grenoble, France
J Cell Sci 123:4201-13. 2010In situ, cells are highly sensitive to geometrical and mechanical constraints from their microenvironment. These parameters are, however, uncontrolled under classic culture conditions, which are thus highly artefactual...
- Biophysical properties of human medulloblastoma cellsNola Jean Ernest
Department of Pediatrics, University of Alabama School of Medicine, 1719 6th Ave S, CIRC 252A, Birmingham, AL 35294, USA
J Membr Biol 237:59-69. 2010..Using patient-derived cell lines and tumor tissues, we show that medulloblastoma cells have no inward Na+ current or transient K+ current involved in action potential generation and propagation, ..
- Metabolic cytometry. Glycosphingolipid metabolism in single cellsColin D Whitmore
Department of Chemistry, University of Washington, Box 351700, Seattle, Washington 98195, USA
Anal Chem 79:5139-42. 2007Glycosphingolipids are found on all vertebrate cells and constitute major cell surface determinants on all nerve cells, where they contribute to cellular diversity and function...
- Development and use of fluorescent nanosensors for metabolite imaging in living cellsM Fehr
Carnegie Institution, Plant Biology, 260 Panama St, Stanford, CA 94305, USA
Biochem Soc Trans 33:287-90. 2005..cameleon and its derivatives were successfully used to follow calcium changes in real time not only in isolated cells, but also in living organisms...
- Doublesex and mab-3-related transcription factor 5 promotes midbrain dopaminergic identity in pluripotent stem cells by enforcing a ventral-medial progenitor fateNicole Gennet
Medical Research Council Clinical Sciences Centre, Faculty of Medicine, Imperial College London, London W12 0NN, United Kingdom
Proc Natl Acad Sci U S A 108:9131-6. 2011..Together, these data identify Dmrt5 as an important player in ventral mesencephalic neural fate specification...
- Dynamic multiphoton imaging: a live view from cells to systemsGrace E Stutzmann
University of California Irvine, Irvine, California, USA
Physiology (Bethesda) 20:15-21. 2005..recent application of multiphoton microscopy to the biological sciences, leading to a new generation of imaging-based studies extending from the tracking of individual molecules within living cells to the observation of whole organisms.
- A method for producing transgenic cells using a multi-integrase system on a human artificial chromosome vectorShigeyuki Yamaguchi
Department of Biomedical Science, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Sciences, Tottori University, Yonago, Japan
PLoS ONE 6:e17267. 2011The production of cells capable of expressing gene(s) of interest is important for a variety of applications in biomedicine and biotechnology, including gene therapy and animal transgenesis...
- New advances on critical implications of tumor- and metastasis-initiating cells in cancer progression, treatment resistance and disease recurrenceM Mimeault
Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, NE 68198 5870, USA
Histol Histopathol 25:1057-73. 2010..evidence have revealed that the malignant transformation of multipotent tissue-resident adult stem/progenitor cells into cancer stem/progenitor cells endowed with a high self-renewal capacity and aberrant multilineage ..
- Global analysis of Förster resonance energy transfer in live cells measured by fluorescence lifetime imaging microscopy exploiting the rise time of acceptor fluorescenceSergey P Laptenok
Laboratory of Biophysics, Wageningen University, Dreijenlaan 3 6703 HA Wageningen, The Netherlands
Phys Chem Chem Phys 12:7593-602. 2010A methodology is described for the quantitative determination of Förster resonance energy transfer (FRET) in live cells using the rise time of acceptor fluorescence as determined with fluorescence lifetime imaging microscopy (FLIM)...
- Pyridine nucleotide cycling and control of intracellular redox state in relation to poly (ADP-ribose) polymerase activity and nuclear localization of glutathione during exponential growth of Arabidopsis cells in cultureTill K Pellny
Plant Sciences, Rothamsted Research, Harpenden, Herts, AL5 2 JQ, UK
Mol Plant 2:442-56. 2009Pyridine nucleotides, ascorbate and glutathione are major redox metabolites in plant cells, with specific roles in cellular redox homeostasis and the regulation of the cell cycle...
- Preparation of cells and tissues for immuno EMPaul Webster
Cell Biology and Biophysics Unit, EMBL, Heidelberg, Meyerhofstrasse 69117, Germany
Methods Cell Biol 88:45-58. 2008..cryosections through chemically-fixed specimens (the Tokuyasu method) is also a popular method for preparing cells and tissues for TEM analysis...
- The defined presentation of nanoparticles to cells and their surface controlled uptakeAlicia Piera Alberola
Fakultat fur Physik and Center for NanoScience CeNS, Ludwig Maximilians Universitat, Geschwister Scholl Platz 1, D 80539 Munich, Germany
Biomaterials 30:3766-70. 2009New approaches and standardized test procedures to study the impact of nanoparticles (NPs) on living cells are urgently needed for the evaluation of potential hazards relating human exposure to NPs...
- Surface plasmon resonance imaging of cells and surface-associated fibronectinAlexander W Peterson
Cell and Tissue Measurements Group, Biochemical Sciences Division, National Institute of Standards and Technology, Gaithersburg, MD, USA
BMC Cell Biol 10:16. 2009A critical challenge in cell biology is quantifying the interactions of cells with their extracellular matrix (ECM) environment and the active remodeling by cells of their ECM...
- Biochemical insights into the mechanisms central to the response of mammalian cells to cold stress and subsequent rewarmingAnne Roobol
Protein Science Group, Department of Biosciences, University of Kent, Canterbury, UK
FEBS J 276:286-302. 2009Mammalian cells cultured in vitro are able to recover from cold stress. However, the mechanisms activated during cold stress and recovery are still being determined...
- Fluorescence correlation spectroscopy measures molecular transport in cellsE L Elson
Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, MO 63110, USA
Traffic 2:789-96. 2001Fluorescence correlation spectroscopy (FCS) can measure dynamics of fluorescent molecules in cells. FCS measures the fluctuations in the number of fluorescent molecules in a small volume illuminated by a thin beam of excitation light...
- Atomic force microscopy imaging of living cells: a preliminary study of the disruptive effect of the cantilever tip on cell morphologyH X You
Department of Cell Biology, Neurobiology and Anatomy, College of Medicine, University of Cincinnati, OH 45267 0521, USA
Ultramicroscopy 82:297-305. 2000..of cell morphological changes generally occurred under normal AFM imaging conditions and to find out how long cells remain viable during scanning...
Research Grants154 found, 100 shown here
- EXOCYTOSIS: USING NANOSURGERY AND BIOENGINEERINGBHANU JENA; Fiscal Year: 2003..called 'pits' 8and 'depressions', have been identified and implicated in exocytosis in live pancreatic acinar cells. Our immediate research goal is to characterize and determine the involvement of these structures in the ..
- UNDERSTANDING MEMBRANE FUSION A BIOENGINEERING APPROACHBHANU JENA; Fiscal Year: 2004..Impairment of the exocytotic process in cells is involved in a number of disease states...
- PROBES FOR FLOW CYTOMETRYZbigniew Darzynkiewicz; Fiscal Year: 1999..objective of this project is the development of new probes, methods and instrumentation for analysis of individual cells by flow or image cytometry, applicable for tumor diagnosis and/or prognosis; the developed methodologies will be ..
- PROBES FOR CYTOMETRYZbigniew Darzynkiewicz; Fiscal Year: 2010..of this project is to continue the development of new bioprobes and methodologies for analysis of individual cells by flow and image cytometry...
- PROBES FOR FLOW CYTOMETRYZbigniew Darzynkiewicz; Fiscal Year: 1993..and molecular probes and techniques to measure content and/or conformation of various constituents of individual cells. These probes (techniques) will be designed for cell analysis by flow cytometry and cell sorting and are expected ..
- Probing single proteins in nanofabricated bioenvironmentDaniel Chiu; Fiscal Year: 2006..We hypothesize that the rate and mechanisms of enzymatic actions and protein interactions in vivo inside cells differ significantly from the rate and mechanisms observed in vitro inside cuvettes or other macroscopic ..
- Cancer Risks Attributable To Radiation from Pediatric CTDavid Brenner; Fiscal Year: 2006..The target audiences will be radiologists, pediatricians, family physicians, and the CT industry. We will use telephone interviews supplemented with survey questions, to aid in web content decisions. ..
- RADIOLOGICAL RESEARCH ACCELERATOR FACILITY (RARAF)David Brenner; Fiscal Year: 2007..to allow time-lapse fluorescent imaging of early (scale of seconds to minutes) radiation events in individual live cells, a new imaging system based on quantitative phase microscopy to image live cells and cell nuclei without use of ..
- NMR and NIR detection of phospholipase activity in vivoEDWARD DELIKATNY; Fiscal Year: 2004..with the differentiating agent phenylbutryate (PB) will lead to increased NIR fluorescence in the prostate tumor cells Aim 3): Demonstration of in vivo delivery and subsequent activation of phospholipases accompanying treatment of ..
- Nutritional Regulation of Ribosomal Protein ExpressionMichael Kilberg; Fiscal Year: 2005Mammalian cells respond to amino acid deprivation by increasing the transcription of a wide variety of genes by a pathway that will be referred to as the Amino Acid Response (AAR)...
- Single-Molecule Immunoassay and DNA ScreeningEdward Yeung; Fiscal Year: 2005We will develop novel imaging technologies for real-time comprehensive analysis of molecular alterations in cells and tissues appropriate for automation and adaptation to high-throughput applications...
- Nutritional Control of Transcription Factor ExpressionMichael Kilberg; Fiscal Year: 2007Mammalian cells respond to nutritional limitation of protein/amino acids by increasing the expression of a wide spectrum of proteins via a signaling pathway that will be referred to as the Amino Acid Response (AAR)...
- NMR detection of tumor differentiationEDWARD DELIKATNY; Fiscal Year: 2009..PB), two differentiating agents currently under Phase II clinical trials, inhibit the proliferation of cancer cells. We will concentrate on the changes in phosphatidylcholine (PtdCho) metabolites that can be observed using in vivo ..
- Single-Molecule Studies of Synaptic VesiclesDaniel Chiu; Fiscal Year: 2009....
- High-Resolution Analysis of Synaptic ProteinsDaniel Chiu; Fiscal Year: 2009..4. To examine the effects of aberrant synaptic activity on vesicular protein composition. ..
- Nutritional Control of Transcription Factor ExpressionMichael S Kilberg; Fiscal Year: 2010Mammalian cells respond to nutritional limitation of protein/amino acids by increasing the expression of a wide spectrum of proteins via a signaling pathway that will be referred to as the Amino Acid Response (AAR)...
- Metabolic Control in a Dynamic EnvironmentJEFF M HASTY; Fiscal Year: 2010In order to optimally control such functions as cellular growth and proliferation, cells must be able to efficiently respond to a dynamically changing environment...
- NMR and NIR detection of phospholipase activity in vivoEdward J Delikatny; Fiscal Year: 2010..PUBLIC HEALTH RELEVANCE: To develop near-infrared fluorescent beacons for the in vivo detection of phospholipase activity via dual optical imaging and MR spectroscopy. ..
- Single-Molecule Studies of Synaptic VesiclesDaniel T Chiu; Fiscal Year: 2010..abstract_text> ..
- Profiling Biochemical Contents of Single MitochondriaDaniel Chiu; Fiscal Year: 2009..for generating over 90% of the cell's ATP, are implicated in aging (the site of oxidative damage in cells), and are found to play a key role in apoptosis and calcium homeostasis...
- Nutritional Control of Transcription Factor ExpressionMichael Kilberg; Fiscal Year: 2009Mammalian cells respond to nutritional limitation of protein/amino acids by increasing the expression of a wide spectrum of proteins via a signaling pathway that will be referred to as the Amino Acid Response (AAR)...
- Development and validation of models for gene regulationJeff Hasty; Fiscal Year: 2007..and focusing both modeling and experimental efforts on determining the subsystems' behavior within living cells in real-time...
- High-Resolution Analysis of Synaptic ProteinsDaniel T Chiu; Fiscal Year: 2010..detail, there is a growing need for quantitative approaches to determine how changes in protein expression in cells affect organelle composition and function...
- Profiling individual synaptic vesicles with nanofluidicsDaniel Chiu; Fiscal Year: 2003....
- High Stability Accelerator for Sub-Cellular MicrobeamDavid Brenner; Fiscal Year: 2003..is the "front end" of our single particle I single cell microbeam which allows irradiation of individual cells and, increasingly, sub-cellular components...
- RADIOLOGICAL RESEARCH ACCELERATOR FACILITY (RARAF)David Brenner; Fiscal Year: 2002..abstract_text> ..
- High-Resolution Analysis of Synaptic ProteinsDaniel T Chiu; Fiscal Year: 2010..detail, there is a growing need for quantitative approaches to determine how changes in protein expression in cells affect organelle composition and function...
- Profiling Biochemical Contents of Single MitochondriaDaniel Chiu; Fiscal Year: 2007..responsible for generating over 90% of the cell's ATP, are implicated in aging (the site of oxidative damage in cells), and are found to play a key role in apoptosis and calcium homeostasis...
- Spatially and Temporally Resolved Perturbation of CellsDaniel Chiu; Fiscal Year: 2007..b>Cells respond to their environment through a complex and interdependent series of signal transduction pathways that ..
- Super-Resolution Imaging with Difference Deconvolution MicroscopyDaniel T Chiu; Fiscal Year: 2010..the best confocal microscope) owing to the diffraction limit, but provides exquisite dynamical information on live cells. With the development of novel fluorescent sensors and molecules in the past decade, the usage of fluorescence ..
- The role of autophagy in innate anti-viral immunity in DrosophilaSara Cherry; Fiscal Year: 2010..These responses include cell-intrinsic mechanisms to combat these invaders in infected cells. Autophagy, the mechanism by which cells engulf cytoplasmic components, has been shown to have antimicrobial ..
- Microfluidics-based Selections for the Optimization of Red Flourescent ProteinsAMY E contact PALMER; Fiscal Year: 2010..enzyme activities, gene expression, and to quantify important signaling molecules in real time in living cells. As a result, fluorescent proteins have truly revolutionized cell biology, shedding light on the basic biology of ..
- MOLECULAR IMAGING OF BIOMATERIALSNicholas Winograd; Fiscal Year: 1993..In addition, the molecular microprobe will be developed for imaging phospholipids in erythrocyte, sperm and nerve cells to correlate membrane structure with function...
- Molecular control of tissue morphogenesisJennifer A Zallen; Fiscal Year: 2010..Cell intercalation is a conserved morphogenetic process involving hundreds of cells that generates one of the striking properties of embryonic form - the body axis...
- Mechanisms of amphipathic and cargo delivery peptidesPaulo Almeida; Fiscal Year: 2005..amphipathic peptides that are antibiotic or cytolytic, or can carry other toxic molecules as cargo into cells. Critical knowledge in reaching this objective is the determination of the mechanism(s) of membrane penetration by ..
- Genetically encoded sensors shed light on zinc homeostasisAmy Palmer; Fiscal Year: 2009..Paradoxically, these essential metals are also toxic and therefore cells must tightly regulate metal accumulation, transport, distribution and export...