Genomes and Genes
Summary: The fundamental, structural, and functional units or subunits of living organisms. They are composed of CYTOPLASM containing various ORGANELLES and a CELL MEMBRANE boundary.
Publications260 found, 100 shown here
- A simple salting out procedure for extracting DNA from human nucleated cellsS A Miller
Memorial Blood Center of Minneapolis, MN 55404
Nucleic Acids Res 16:1215. 1988
- Assessing mitochondrial dysfunction in cellsMartin D Brand
Buck Institute for Research on Aging, 8001 Redwood Blvd, Novato, CA 94945, USA
Biochem J 435:297-312. 2011..Dysfunction can be assessed in isolated mitochondria, in cells or in vivo, with different balances between precise experimental control and physiological relevance...
- Clearance of apoptotic cells: implications in health and diseaseMichael R Elliott
Center for Cell Clearance and the Department of Microbiology, University of Virginia, Charlottesville, VA 22908, USA
J Cell Biol 189:1059-70. 2010Recent advances in defining the molecular signaling pathways that regulate the phagocytosis of apoptotic cells have improved our understanding of this complex and evolutionarily conserved process...
- Microautophagy in mammalian cells: revisiting a 40-year-old conundrumDalibor Mijaljica
Department of Biochemistry and Molecular Biology, School of Biomedical Sciences, Monash University, Victoria, Australia
Autophagy 7:673-82. 2011..de Duve and Wattiaux and subsequently applied, over the following two decades, to processes described in mammalian cells and involving the presence of lysosome-like organelles having multiple vesicles trapped in their lumen ("..
- Central dogma at the single-molecule level in living cellsGene Wei Li
Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, USA
Nature 475:308-15. 2011Gene expression originates from individual DNA molecules within living cells. Like many single-molecule processes, gene expression and regulation are stochastic, that is, sporadic in time...
- Nonsense-mediated mRNA decay in human cells: mechanistic insights, functions beyond quality control and the double-life of NMD factorsPamela Nicholson
Institute of Cell Biology, University of Bern, Baltzerstrasse 4, 3012, Bern, Switzerland
Cell Mol Life Sci 67:677-700. 2010..In recent years, the discovery of additional functions played by several of the NMD factors has further complicated the picture. Therefore, we also review the reported roles of UPF1, SMG1 and SMG6 in other cellular processes...
- Molecular identification of a danger signal that alerts the immune system to dying cellsYan Shi
Department of Pathology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
Nature 425:516-21. 2003..Danger signals are thought to act by stimulating dendritic cells to mature so that they can present foreign antigens and stimulate T lymphocytes...
- From cells to organs: building polarized tissueDavid M Bryant
Department of Anatomy, University of California San Francisco, California 94143 2140, USA
Nat Rev Mol Cell Biol 9:887-901. 2008How do animal cells assemble into tissues and organs? A diverse array of tissue structures and shapes can be formed by organizing groups of cells into different polarized arrangements and by coordinating their polarity in space and time...
- A genetically encoded tag for correlated light and electron microscopy of intact cells, tissues, and organismsXiaokun Shu
Howard Hughes Medical Institute, University of California at San Diego, La Jolla, California, United States of America
PLoS Biol 9:e1001041. 2011..specific protein EM labeling has lacked generally applicable genetically encoded tags for in situ visualization in cells and tissues...
- The regulation of extracellular signal-regulated kinase (ERK) in mammalian cellsJoe W Ramos
Department of Natural Products and Cancer Biology, Cancer Research Center of Hawaii, University of Hawaii at Manoa, 651 Ilalo Street, Honolulu, HI 96813, USA
Int J Biochem Cell Biol 40:2707-19. 2008..Finally, several feedback mechanisms have been identified downstream of ERK activation. Here we will focus on the diverse mechanisms of ERK regulation in mammalian cells.
- Reprogramming of murine and human somatic cells using a single polycistronic vectorBryce W Carey
Whitehead Institute for Biomedical Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02142, USA
Proc Natl Acad Sci U S A 106:157-62. 2009Directed reprogramming of somatic cells by defined factors provides a novel method for the generation of patient-specific stem cells with the potential to bypass both the practical and ethical concerns associated with somatic cell ..
- Identification of genetic variants that affect histone modifications in human cellsGraham McVicker
Department of Human Genetics, University of Chicago, Chicago, IL 60637, USA
Science 342:747-9. 2013..Furthermore, variants that affect chromatin at distal regulatory sites frequently also direct changes in chromatin and gene expression at associated promoters. ..
- Electron tomography of cellsLu Gan
Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA
Q Rev Biophys 45:27-56. 2012..to become the highest resolution three-dimensional (3D) imaging technique available for unique objects such as cells. Cells can be imaged either plastic-embedded or frozen-hydrated...
- Second-harmonic imaging microscopy for visualizing biomolecular arrays in cells, tissues and organismsPaul J Campagnola
Center for Biomedical Imaging Technology, Department of Physiology, University of Connecticut Health Center, Farmington, Connecticut 06030, USA
Nat Biotechnol 21:1356-60. 2003..Better dyes and optimized microscope optics could ultimately lead to the imaging of neuronal electrical activity with SHIM...
- Direct effects of type I interferons on cells of the immune systemSandra Hervas-Stubbs
Division of Gene Therapy and Hepatology, Centre for Applied Medical Research CIMA, University of Navarra, Pamplona, Spain
Clin Cancer Res 17:2619-27. 2011..induces the Janus activated kinase-signal transducer and activation of transcription (JAK-STAT) pathway in most cells, along with other biochemical pathways that may differentially operate, depending on the responding cell subset, ..
- Interaction of nanoparticles with cellsVolker Mailänder
Max Planck Institute for Polymer Research, Ackermannweg 10, 55128 Mainz, Germany
Biomacromolecules 10:2379-400. 2009Nanoparticles and their interaction with human cells have been a focus of many groups during the past decade...
- There is no evidence that mitochondria are the main source of reactive oxygen species in mammalian cellsGuy C Brown
Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1QW, UK
Mitochondrion 12:1-4. 2012It is often assumed that mitochondria are the main source of reactive oxygen species (ROS) in mammalian cells, but there is no convincing experimental evidence for this in the literature...
- Dynamics of single mRNP nucleocytoplasmic transport and export through the nuclear pore in living cellsAmir Mor
The Mina and Everard Goodman Faculty of Life Sciences and Institute of Nanotechnology, Bar Ilan University, Ramat Gan 52900, Israel
Nat Cell Biol 12:543-52. 2010The flow of genetic information in eukaryotic cells occurs through the nucleocytoplasmic translocation of mRNAs. Knowledge of in vivo messenger RNA export kinetics remains poor in comparison with that of protein transport...
- Structural studies by electron tomography: from cells to moleculesVladan Lucic
Department of Structural Biology, Max Planck Institute of Biochemistry, D 82152 Martinsried, Germany
Annu Rev Biochem 74:833-65. 2005..tomography (ET) is uniquely suited to obtain three-dimensional reconstructions of pleomorphic structures, such as cells, organelles or supramolecular assemblies...
- Evidence for transcript networks composed of chimeric RNAs in human cellsSarah Djebali
Bioinformatics and Genomics, Centre for Genomic Regulation and Universitat Pompeu Fabra, Barcelona, Catalonia, Spain
PLoS ONE 7:e28213. 2012..The non-random nature of the connection of the genes involved suggest that chimeric transcripts should not be studied in isolation, but together, as an RNA network...
- Mobile DNA transposition in somatic cellsHaig H Kazazian
Institute for Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
BMC Biol 9:62. 2011..germ-cell development rather than in somatic-cell development, but solid evidence for transposition in somatic cells is now accumulating...
- Artificial induction of autophagy around polystyrene beads in nonphagocytic cellsShouhei Kobayashi
Kobe Advanced ICT Research Center, National Institute of Information and Communications Technology, Iwaoka cho, Nishi ku, Kobe, Japan
Autophagy 6:36-45. 2010..defense mechanism to kill invading bacteria such as group A Streptococcus in nonphagocytic epithelial-like cells. The cellular events underlying autophagosome formation upon bacterial invasion remain unclear due to the ..
- Compatibility of mammalian cells on surfaces of poly(dimethylsiloxane)Jessamine Ng Lee
Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, Massachusetts 02138
Langmuir 20:11684-91. 2004..composition of poly(dimethylsiloxane) (PDMS) on the attachment and growth of several different types of mammalian cells: primary human umbilical artery endothelial cells (HUAECs), transformed 3T3 fibroblasts (3T3s), transformed ..
- Silver nanospheres are cytotoxic and genotoxic to fish cellsJohn Pierce Wise
Wise Laboratory of Environmental and Genetic Toxicology, University of Southern Maine, 96 Falmouth St, P O Box 9300, Portland, ME 04104, USA
Aquat Toxicol 97:34-41. 2010..1 and 0.3microg/cm(2) induced damage in 8, 10.8, 16 and 15.8% of metaphases and 10.8, 15.6, 24 and 24 total aberrations in 100 metaphases, respectively. These data show that silver nanoparticles are cytotoxic and genotoxic to fish cells.
- Effector cells of both nonhemopoietic and hemopoietic origin are required for interferon (IFN)-gamma- and tumor necrosis factor (TNF)-alpha-dependent host resistance to the intracellular pathogen, Toxoplasma gondiiG S Yap
Immunobiology Section, Laboratory of Parasitic Diseases, National Institutes of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
J Exp Med 189:1083-92. 1999..intracellular pathogens, it is unclear how they control the growth of microorganisms that reside in nonhemopoietic cells. Pathogens within such cells may be killed by metabolites secreted by activated macrophages or, alternatively, ..
- Oxidative protein folding in the secretory pathway and redox signaling across compartments and cellsEva Margittai
Department of Medical Chemistry, Molecular Biology and Pathobiochemistry, Semmelweis University, Budapest, Hungary
Traffic 12:1-8. 2011....
- Non-genetic heterogeneity of cells in development: more than just noiseSui Huang
Institute for Biocomplexity and Informatics, Biological Sciences Bldg, Room 539D, University of Calgary, Calgary, AB T3A 0Y1, Canada
Development 136:3853-62. 2009Cell-to-cell variability of gene expression in clonal populations of mammalian cells is ubiquitous...
- Primary and secondary mechanisms of action of visible to near-IR radiation on cellsT Karu
Laser Technology Research Center of Russian Academy of Sciences, Troitsk, Moscow Region, Russia
J Photochem Photobiol B 49:1-17. 1999Cytochrome c oxidase is discussed as a possible photoacceptor when cells are irradiated with monochromatic red to near-IR radiation...
- HITS-CLIP: panoramic views of protein-RNA regulation in living cellsRobert B Darnell
Laboratory of Neuro Oncology, The Rockefeller University, Howard Hughes Medical Institute, New York, NY 10065, USA
Wiley Interdiscip Rev RNA 1:266-86. 2010The study of gene regulation in cells has recently begun to shift from a period dominated by the study of transcription factor-DNA interactions to a new focus on RNA regulation...
- Secretory protein trafficking and organelle dynamics in living cellsJ Lippincott-Schwartz
Cell Biology and Metabolism Branch, NICHD, National Institutes of Health, Bethesda, Maryland 20892, USA
Annu Rev Cell Dev Biol 16:557-89. 2000..acting as reporters for protein localization and trafficking within the secretory membrane system of living cells have been used in a wide variety of applications, including time-lapse imaging, double-labeling, energy transfer, ..
- Chapter 9: Counting proteins in living cells by quantitative fluorescence microscopy with internal standardsJian Qiu Wu
Department of Molecular Genetics, The Ohio State University, Columbus, Ohio 43210, USA
Methods Cell Biol 89:253-73. 2008..microscope can be treated as a spectrophotometer to measure the absolute number of fluorescent molecules in live cells (Wu and Pollard, 2005)...
- Rewiring cells: synthetic biology as a tool to interrogate the organizational principles of living systemsCaleb J Bashor
Department of Cellular and Molecular Pharmacology, University of California, San Francisco, California 94158 2517, USA
Annu Rev Biophys 39:515-37. 2010....
- Programmed necrosis induced by asbestos in human mesothelial cells causes high-mobility group box 1 protein release and resultant inflammationHaining Yang
Cancer Research Center of Hawaii, John A Burns School of Medicine, University of Hawaii, Honolulu, HI 96813, USA
Proc Natl Acad Sci U S A 107:12611-6. 2010..has been linked to the release of cytokines and mutagenic reactive oxygen species (ROS) from inflammatory cells. Asbestos is cytotoxic to human mesothelial cells (HM), which appears counterintuitive for a carcinogen...
- A method for producing transgenic cells using a multi-integrase system on a human artificial chromosome vectorShigeyuki Yamaguchi
Department of Biomedical Science, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Sciences, Tottori University, Yonago, Japan
PLoS ONE 6:e17267. 2011The production of cells capable of expressing gene(s) of interest is important for a variety of applications in biomedicine and biotechnology, including gene therapy and animal transgenesis...
- Engineered cobalt oxide nanoparticles readily enter cellsElena Papis
Department of Biotechnology and Molecular Science, Insubria University, Varese, Italy
Toxicol Lett 189:253-9. 2009..To determine their potential toxicity to human cells, we measured cell viability and ROS formation in two human cell lines using CoCl2 for comparison...
- Origin of first cells at terrestrial, anoxic geothermal fieldsArmen Y Mulkidjanian
School of Physics, University of Osnabruck, D 49069 Osnabruck, Germany
Proc Natl Acad Sci U S A 109:E821-30. 2012All cells contain much more potassium, phosphate, and transition metals than modern (or reconstructed primeval) oceans, lakes, or rivers...
- Nanolitre liquid patterning in aqueous environments for spatially defined reagent delivery to mammalian cellsH Tavana
Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan 48109, USA
Nat Mater 8:736-41. 2009..These existing methods, therefore, are limited for patterning onto delicate surfaces of living cells because physical contact or substantially dry conditions are damaging to them...
- Role of sulfatide in normal and pathological cells and tissuesTadanobu Takahashi
Department of Biochemistry, School of Pharmaceutical Sciences, University of Shizuoka and Global COE Program for Innovation in Human Health Sciences, 52 1 Yada, Suruga ku, Shizuoka shi, Shizuoka 422 8526, Japan
J Lipid Res 53:1437-50. 2012....
- On the evolution of cellsCarl R Woese
Department of Microbiology, University of Illinois at Urbana Champaign, 601 South Goodwin Avenue, B103 Chemical and Life Sciences Laboratory, Urbana, IL 61801 3709, USA
Proc Natl Acad Sci U S A 99:8742-7. 2002..Primitive cells did not carry a stable organismal genealogical trace. Primitive cellular evolution is basically communal...
- Highly parallel genome-wide expression analysis of single mammalian cellsJian Bing Fan
Research and Development, Illumina, Inc, San Diego, California, United States of America
PLoS ONE 7:e30794. 2012..We have developed a high-throughput amplification method for generating robust gene expression profiles using single cell or low RNA inputs...
- Pancreatic stellate cells: new target in the treatment of chronic pancreatitisRupjyoti Talukdar
Department of Gastroenterology, Pushpawati Singhania Research Institute, New Delhi, India
J Gastroenterol Hepatol 23:34-41. 2008..Recent in vitro and in vivo experiments have proven objectively the role of activated pancreatic stellate cells (PSC) in fibrogenesis in CP...
- Population dynamics in vasopressin cellsGareth Leng
Centre for Integrative Physiology, University of Edinburgh, Edinburgh, UK
Neuroendocrinology 88:160-72. 2008..Vasopressin cells sense plasma osmolality and secrete appropriate levels of vasopressin from the neurohypophysis as needed to ..
- Death by protein damage in irradiated cellsMichael J Daly
Department of Pathology, Uniformed Services University of the Health Sciences USUHS, School of Medicine, Bethesda, MD 20814, USA
DNA Repair (Amst) 11:12-21. 2012..Mounting experimental evidence does not fit into this theoretical framework. Whereas DNA lesion-yields in cells exposed to a given dose and type of radiation appear to be fixed, protein lesion-yields are highly variable...
- Diverse apoptotic pathways in enterovirus 71-infected cellsShih Cheng Chang
Graduate Institute of Basic Medical Sciences, Chang Gung University, Tao Yuan, Taiwan
J Neurovirol 10:338-49. 2004..This investigation conducts a comprehensive study of the apoptotic pathways in neural and non-neural cells following enterovirus 71 infection...
- Auxin influx inhibitors 1-NOA, 2-NOA, and CHPAA interfere with membrane dynamics in tobacco cellsMartina Lanková
Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Rozvojova 263, CZ 165 02 Prague 6, Czech Republic
J Exp Bot 61:3589-98. 2010..The results suggest that these, previously unknown, activities of putative auxin influx inhibitors regulate overall auxin transport across the plasma membrane depending on the dynamics of particular membrane vesicles...
- Long noncoding RNAs in mammalian cells: what, where, and why?Ling Ling Chen
Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, CT 06030, USA
Wiley Interdiscip Rev RNA 1:2-21. 2010..Here, we discuss some recent progress in our understanding of the functions of a number of important lncRNAs in mammalian cells.
- Intracellular fluid flow in rapidly moving cellsKinneret Keren
Department of Biochemistry and Howard Hughes Medical Institute, Stanford University School of Medicine, Stanford, California 94305, USA
Nat Cell Biol 11:1219-24. 2009..To investigate the existence and the direction of fluid flow in rapidly moving cells, we introduced inert quantum dots into the lamellipodia of fish epithelial keratocytes and analysed their ..
- The role of vinculin in the regulation of the mechanical properties of cellsClaudia Tanja Mierke
Center for Medical Physics and Technology, Biophysics Group, Friedrich Alexander University of Erlangen Nuremberg, Henkestrasse 91, Erlangen, 91052, Germany
Cell Biochem Biophys 53:115-26. 2009..an inhibitor of cell migration on planar substrates, because knock-out of vinculin in F9 mouse embryonic carcinoma cells and mouse embryonic fibroblasts showed increased cell motility on 2D substrates...
- Mechanisms of miRNA-mediated post-transcriptional regulation in animal cellsMarina Chekulaeva
Friedrich Miescher Institute for Biomedical Research, 4002 Basel, Switzerland
Curr Opin Cell Biol 21:452-60. 2009..In this report, we review current progress in understanding how miRNAs execute these effects in animals and we discuss some of the controversies regarding different modes of miRNA function...
- How can biochemical reactions within cells differ from those in test tubes?Allen P Minton
Section on Physical Biochemistry, Laboratory of Biochemical Pharmacology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health U S DHHS, Bethesda, MD 20892, USA
J Cell Sci 119:2863-9. 2006..Greater than order-of-magnitude increases in association rate and equilibrium constants attributable to background interactions have been observed in simulated and actual intracellular environments...
- Discovery of new cargo proteins that enter cells through clathrin-independent endocytosisCraig A Eyster
Laboratory of Cell Biology, NHLBI, National Institutes of Health, Bethesda, Maryland 20892, USA
Traffic 10:590-9. 2009..lacking clathrin-targeting sequences, such as the major histocompatibility complex class I protein (MHCI), into cells. After internalization, vesicles containing MHCI fuse with transferrin-containing endosomes generated from ..
- Microfluidic devices for measuring gene network dynamics in single cellsMatthew R Bennett
Department of Biochemistry and Cell Biology and Institute of Biosciences and Bioengineering, Rice University, 6100 Main Street, Houston, Texas 77005 1892, USA
Nat Rev Genet 10:628-38. 2009..Recent technological advances now make it possible to track the dynamics of gene networks in single cells under various environmental conditions using microfluidic 'lab-on-a-chip' devices, and researchers are using these ..
- Physical limits of cells and proteomesKen A Dill
Laufer Center for Physical and Quantitative Biology and Department of Physics, Stony Brook University, New York, NY 11794, USA
Proc Natl Acad Sci U S A 108:17876-82. 2011..Why do mesophilic cells die around 50 °C? How can the maximal growth-rate temperature (around 37 °C) occur so close to the cell-..
- An ezrin-rich, rigid uropod-like structure directs movement of amoeboid blebbing cellsAnna Lorentzen
Institute of Cancer Research, Cancer Research UK, Cancer Research UK Tumour Cell Signalling Unit, 237 Fulham Road, London SW3 6JB, UK
J Cell Sci 124:1256-67. 2011Melanoma cells can switch between an elongated mesenchymal-type and a rounded amoeboid-type migration mode. The rounded 'amoeboid' form of cell movement is driven by actomyosin contractility resulting in membrane blebbing...
- Splicing-independent recruitment of U1 snRNP to a transcription unit in living cellsBéatrice Spiluttini
Ecole Normale Superieure, Institut de Biologie de l ENS, IBENS, Paris, France
J Cell Sci 123:2085-93. 2010..The constitutive association of U1 small nuclear ribonucleoprotein (snRNP) with the transcription machinery might play a role in coupling transcription with pre-mRNA maturation...
- How and where are nonsense mRNAs degraded in mammalian cells?Oliver Mühlemann
Institute of Cell Biology, University of Bern, Bern, Switzerland
RNA Biol 7:28-32. 2010..Here we discuss recent evidence as to how and where mRNAs targeted to NMD are degraded in human cells. We discuss accumulating evidence that the decay step of human NMD can be initiated by two different mechanisms: ..
- Shank-interacting protein-like 1 promotes tumorigenesis via PTEN inhibition in human tumor cellsLizhi He
Division of Nephrology, Department of Medicine, McMaster University, Hamilton, Ontario, Canada
J Clin Invest 120:2094-108. 2010..shank-interacting protein-like 1 (SIPL1) as a PTEN-NR in human tumor cell lines and human primary cervical cancer cells. Ectopic SIPL1 expression protected human U87 glioma cells from PTEN-mediated growth inhibition and promoted the ..
- Measuring enzyme activity in single cellsMichelle L Kovarik
Department of Chemistry, University of North Carolina, Chapel Hill, NC 27599, USA
Trends Biotechnol 29:222-30. 2011Seemingly identical cells can differ in their biochemical state, function and fate, and this variability plays an increasingly recognized role in organism-level outcomes...
- Confocal/TEM overlay microscopy: a simple method for correlating confocal and electron microscopy of cells expressing GFP/YFP fusion proteinsDouglas R Keene
Research Department, Shriners Hospital for Children, Portland, OR 97239, USA
Microsc Microanal 14:342-8. 2008..of light microscopy and high resolution transmission electron microscopy (TEM) on components within identical cells has been problematic...
- Correlative 3D imaging of whole mammalian cells with light and electron microscopyGavin E Murphy
Center for Cancer Research, National Cancer Institute, NIH, Bethesda, MD 20892, USA
J Struct Biol 176:268-78. 2011..We establish protocols that enable the routine generation of 3D image stacks of entire plastic-embedded mammalian cells by IA-SEM at resolutions of ∼10-20nm at high contrast and with minimal artifacts from the focused ion beam...
- Competition, collaboration and coordination--determining how cells bypass DNA damageJulian E Sale
Medical Research Council Laboratory of Molecular Biology, Cambridge, UK
J Cell Sci 125:1633-43. 2012b>Cells must overcome replication blocks that might otherwise lead to genomic instability or cell death...
- Stochastic protein expression in individual cells at the single molecule levelLong Cai
Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, Massachusetts 02138, USA
Nature 440:358-62. 2006..stochastic events of protein production are difficult to observe directly with measurements on large ensembles of cells owing to lack of synchronization among cells...
- FTIR microscopy of biological cells and tissue: data analysis using resonant Mie scattering (RMieS) EMSC algorithmPaul Bassan
Manchester Interdisciplinary Biocentre, University of Manchester, 131 Princess Street, Manchester, M1 7DN, UK
Analyst 137:1370-7. 2012Transmission and transflection infrared microscopy of biological cells and tissue suffer from significant baseline distortions due to scattering effects, predominantly resonant Mie scattering (RMieS)...
- Comparison of segmentation algorithms for fluorescence microscopy images of cellsAlden A Dima
Software and Systems Division, Information Technology Laboratory, National Institute of Standards and Technology, Gaithersburg, Maryland 20899, USA
Cytometry A 79:545-59. 2011The analysis of fluorescence microscopy of cells often requires the determination of cell edges. This is typically done using segmentation techniques that separate the cell objects in an image from the surrounding background...
- Molecular and structural basis for N-glycan-dependent determination of glycoprotein fates in cellsYukiko Kamiya
Okazaki Institute for Integrative Bioscience and Institute for Molecular Science, National Institutes of Natural Sciences, 5 1 Higashiyama, Myodaiji, Okazaki, Aichi 444 8787, Japan
Biochim Biophys Acta 1820:1327-37. 2012..e. folding in the endoplasmic reticulum (ER), vesicular transport between the ER and the Golgi complex, and ER-associated degradation of glycoproteins, by interacting with a panel of intracellular lectins in the early secretory pathway...
- Sphingomyelin and sphingomyelin synthase (SMS) in the malignant transformation of glioma cells and in 2-hydroxyoleic acid therapyGwendolyn Barcelo-Coblijn
Laboratory of Molecular Cell Biomedicine, Department of Biology, University of the Balearic Islands, Carretera de Valldemossa Km 7 5, E 07122 Palma, Balearic Islands, Spain
Proc Natl Acad Sci U S A 108:19569-74. 2011..Here, we show that human cancer cells have markedly lower levels of sphingomyelin (SM) than nontumor (MRC-5) cells...
- Advances in tomography: probing the molecular architecture of cellsKaren Fridman
Department of Life Sciences and the National Institute for Biotechnology in the Negev, Ben Gurion University, Beer Sheva, 84105 Israel
Nat Rev Mol Cell Biol 13:736-42. 2012Visualizing the dynamic molecular architecture of cells is instrumental for answering fundamental questions in cellular and structural biology...
- CELLPEDIA: a repository for human cell information for cell studies and differentiation analysesAkiko Hatano
Computational Biology Research Center, Advanced Industrial Science and Technology AIST, 2 4 7 Aomi, Koto ku, Tokyo 135 0064, Japan
Database (Oxford) 2011:bar046. 2011CELLPEDIA is a repository database for current knowledge about human cells. It contains various types of information, such as cell morphologies, gene expression and literature references...
- New views of cells in 3D: an introduction to electron tomographyRichard McIntosh
Laboratory for 3D Electron Microscopy of Cells, Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, CO 80309 0347, USA
Trends Cell Biol 15:43-51. 2005..Electron tomography can provide such information about many kinds of cells and organelles.
- Glutamine breakdown in rapidly dividing cells: waste or investment?J Carlos Aledo
Departamento de Biologia Molecular y Bioquimica, Facultad de Ciencias, Universidad de Malaga, 29071 Malaga, Spain
Bioessays 26:778-85. 2004Tumours, and in general rapidly dividing cells, behave as dissipative devices that apparently waste glutamine, since its consumption seems to exceed both energetic and nitrogen needs...
- Anomalous transport in the crowded world of biological cellsFelix Höfling
Max Planck Institut für Intelligente Systeme, Heisenbergstraße 3, 70569 Stuttgart, and Institut für Theoretische Physik IV, Universitat Stuttgart, Pfaffenwaldring 57, 70569 Stuttgart, Germany
Rep Prog Phys 76:046602. 2013..This is commonly attributed to macromolecular crowding in the interior of cells and in cellular membranes, summarizing their densely packed and heterogeneous structures...
- Influence of the metabolic properties of human cells on the kinetic of formation of the major benzo[a]pyrene DNA adductsCaroline Marie
Laboratoire des Lésions des Acides Nucléiques, LCIB UMR E 3 CEA UJF, DRFMC, CEA Grenoble, 17 Avenue des Martyrs 38054 Grenoble Cedex 9, France
J Appl Toxicol 28:579-90. 2008..of B[a]P exposure, the aim of the study was to identify the major stable DNA adducts in B[a]P-treated human cells. The role played by cellular metabolism on the nature and frequency of the DNA lesions was investigated using ..
- Rapid-on-chip determination of dielectric properties of biological cells using imaging techniques in a dielectrophoresis dot microsystemHenry O Fatoyinbo
Centre for Biomedical Engineering, Faculty of Engineering and Physical Sciences, University of Surrey, Guildford, Surrey, UK
Electrophoresis 29:3-10. 2008..A specific application of this technique is deducing the dielectric properties of cells from analysis of the dielectrophoretic spectrum of that particular cell population...
- The Cinderella syndrome: why do malaria-infected cells burst at midnight?Nicole Mideo
Center for Infectious Disease Dynamics, The Pennsylvania State University, University Park, PA 16802, USA
Trends Parasitol 29:10-6. 2013....
- A novel method for the production of transgenic cloned pigs: electroporation-mediated gene transfer to non-cultured cells and subsequent selection with puromycinSatoshi Watanabe
Department of Developmental Biology, Division of Insect and Animal, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, 305 0901, Japan
Biol Reprod 72:309-15. 2005..Somatic cells isolated from porcine fetuses at 73 days of gestation were immediately electroporated with a transgene (pCAG-..
- Effects of ionizing radiation in nonirradiated cellsWilliam F Morgan
Radiation Oncology Research Laboratory and Marlene and Stewart Greenebaum Cancer Center, University of Maryland, BRB 7 011, Baltimore, MD 21201 1509, USA
Proc Natl Acad Sci U S A 102:14127-8. 2005
- Quantification of PtdInsP3 molecular species in cells and tissues by mass spectrometryJonathan Clark
Inositide Laboratory, Babraham Institute, Babraham Research Campus, Cambridge, UK
Nat Methods 8:267-72. 2011..This methodology can be used to quantify multiple fatty-acyl species of PtdIns(3,4,5)P(3) in unstimulated mouse and human cells (≥10(5)) or tissues (≥0.1 mg) and their increase upon appropriate stimulation.
- Microfluidic devices for the high-throughput chemical analysis of cellsMaxine A McClain
Oak Ridge National Laboratory, P O Box 2008, Oak Ridge, Tennessee 37831 6142, USA
Anal Chem 75:5646-55. 2003..The device function was demonstrated using Jurkat cells that were loaded with the fluorogenic dyes - carboxyfluorescein diacetate, Oregon green carboxylic acid diacetate, ..
- Cell-based electrochemical biosensors for water quality assessmentFlorence Lagarde
University of Lyon, Laboratoire des Sciences Analytiques, UMR, CNRS, CPE, Villeurbanne, France
Anal Bioanal Chem 400:947-64. 2011..b>Cells are easy to cultivate and manipulate, host many enzymes able to catalyse a wide range of biological reactions and ..
- Computer-aided detection of centroblasts for follicular lymphoma grading using adaptive likelihood-based cell segmentationOlcay Sertel
Department of Biomedical Informatics, The Ohio State University, Columbus, OH 43210, USA
IEEE Trans Biomed Eng 57:2613-6. 2010..for FL patients are based on histological grading, which is done by manually counting the large malignant cells called centroblasts (CB) in ten standard microscopic high-power fields from H&E-stained tissue sections...
- Nanosecond, high-intensity pulsed electric fields induce apoptosis in human cellsStephen J Beebe
Center for Pediatric Research, 855 W Brambleton Ave, Norfolk, VA 23510, USA
FASEB J 17:1493-5. 2003..compared with the charging time of the plasma membrane can induce cell fusion or introduce xenomolecules into cells. Nanosecond pulse power technology generates pulses with high-intensity electric fields, but with such short ..
- Do we underestimate the importance of water in cell biology?Martin Chaplin
Department of Applied Science, London South Bank University, Borough Road, London SE1 0AA, UK
Nat Rev Mol Cell Biol 7:861-6. 2006..Water can generate small active clusters and macroscopic assemblies, which can both transmit information on different scales...
- Treatment with insulin glargine (Lantus) increases the proliferative potency of the serum of patients with type-1 diabetes: a pilot study on MCF-7 breast cancer cellsDoris Mayer
Hormones and Signal Transduction Group, German Cancer Research Centre, DKFZ ZMBH Alliance, Im Neuenheimer Feld 581, Heidelberg, Germany
Arch Physiol Biochem 116:73-8. 2010Insulin glargine (Lantus) stimulates growth of MCF-7 cells stronger than human insulin. We investigated if serum from diabetic patients treated with glargine versus human insulin may display a similar effect.
- High-resolution nonlinear optical imaging of live cells by second harmonic generationP J Campagnola
Department of Physiology and Center for Biomedical Imaging Technology, University of Connecticut Health Center, Farmington, Connecticut 06030 USA
Biophys J 77:3341-9. 1999..Second harmonic imaging (SHIM) is an ideal method for probing membranes of living cells because it offers the high resolution of nonlinear optical microscopy with the potential for near-total avoidance ..
- HIV dynamics with multiple infections of target cellsNarendra M Dixit
Theoretical Biology and Biophysics, Theoretical Division, Los Alamos National Laboratory, Los Alamos, NM 87545, USA
Proc Natl Acad Sci U S A 102:8198-203. 2005The high incidence of multiple infections of cells by HIV sets the stage for rapid HIV evolution by means of recombination. Yet how HIV dynamics proceeds with multiple infections remains poorly understood...
- Analysis of intracellular oxygen and metabolic responses of mammalian cells by time-resolved fluorometryAlexander V Zhdanov
Luxcel Biosciences Ltd, BioInnovation Centre, UCC, Cork, Ireland
Anal Chem 79:9414-9. 2007A simple, minimally invasive methodology for the analysis of intracellular oxygen in populations of live mammalian cells is described...
- Modelling reaction kinetics inside cellsRamon Grima
Institute for Mathematical Sciences, Imperial College, London, U K
Essays Biochem 45:41-56. 2008..molecule imaging techniques have given us a window into the intricate biochemical activities that occur inside cells. In this chapter we review four distinct theoretical and simulation frameworks: (i) non-spatial and deterministic, ..
- Transcriptome and targetome analysis in MIR155 expressing cells using RNA-seqGuorong Xu
Department of Computer Science, University of New Orleans, New Orleans, LA 70148, USA
RNA 16:1610-22. 2010....
- Mechanisms and cellular roles of local protein synthesis in mammalian cellsAlexis J Rodriguez
Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, United States
Curr Opin Cell Biol 20:144-9. 2008..is mRNA subject to translation, but also through RNA-binding proteins and other trans-acting factors, eukaryotic cells interpret codes for spatial sorting within the mRNA sequence...
- Interactions of Listeria monocytogenes with mammalian cells during entry and actin-based movement: bacterial factors, cellular ligands and signalingP Cossart
Unité des Interactions Bactéries Cellules, Institut Pasteur, 28 rue du Docteur Roux, Paris 75015, France
EMBO J 17:3797-806. 1998..The mechanisms that Listeria uses to enter cells, escape from the phagocytic vacuole and spread from one cell to another using an actin-based motility process have ..
- Noncontact measurement of the local mechanical properties of living cells using pressure applied via a pipetteDaniel SÃ¡nchez
Division of Medicine, Imperial College London, Hammersmith Hospital Campus, London, W12 0NN, United Kingdom
Biophys J 95:3017-27. 2008..to apply quantified positive and negative force at defined positions to the soft responsive surface of living cells. The method uses applied hydrostatic pressure (0...
- Biosensors based on the energy metabolism of living cells: the physical chemistry and cell biology of extracellular acidificationJ C Owicki
Molecular Devices Corporation, Menlo Park, CA 94025
Biosens Bioelectron 7:255-72. 1992..is a biosensor-based instrument that detects changes in the physiological state of cultured living cells by monitoring the rate at which the cells excrete acidic products of metabolism...
- Optical techniques for imaging membrane lipid microdomains in living cellsDylan M Owen
Chemical Biology Centre, Imperial College London, London, UK
Semin Cell Dev Biol 18:591-8. 2007..For example, trafficking and secretion of molecules can be targeted and directed, cells polarised and signalling events modulated and propagated...
- Probing nanomechanical properties from biomolecules to living cellsS Kasas
Laboratoire de Physique de la Matière Complexe, EPFL, CH 1015 Lausanne, Switzerland
Pflugers Arch 456:13-27. 2008..In this review, we will focus on the indentation technique and will illustrate its application to biological materials with examples that range from single molecules to living cells.
- Aneuploidy in mitosis of PtK1 cells is generated by random loss and nondisjunction of individual chromosomesLiliana Torosantucci
IBPM Institute of Molecular Biology and Pathology, CNR National Research Council, University La Sapienza, Via degli Apuli 4, 00185 Rome, Italy
J Cell Sci 122:3455-61. 2009..of the six PtK1 chromosomes to undergo nondisjunction and/or chromosome loss was observed in ana-telophase cells recovering from a nocodazole- or a monastrol-induced mitotic arrest, suggesting that the recurrent presence of ..
- Weiss Lecture. Effects of radiations of different qualities on cells: molecular mechanisms of damage and repairD T Goodhead
Medical Research Council Radiobiology Unit, Chilton, Didcot, UK
Int J Radiat Biol 63:543-56. 1993..of ionizing radiations of different quality are discussed with particular emphasis on damage to DNA of mammalian cells. Three related themes are followed...
- Defective interfering RNAs of Japanese encephalitis virus found in mosquito cells and correlation with persistent infectionKuen Nan Tsai
Institute of Biotechnology, Department of Life Science, National Dong Hwa University, Taiwan, ROC
Virus Res 124:139-50. 2007..we have identified for the first time the generation of DI RNAs of Japanese encephalitis virus in C6/36 mosquito cells. A persistent infection was established by replacing growth medium on surviving cells and continued cell passaging...
- SCART scavenger receptors identify a novel subset of adult gammadelta T cellsJan Kisielow
Molecular Biomedicine, Institute of Integrative Biology, Swiss Federal Institute of Technology ETH, Zurich Schlieren, Switzerland
J Immunol 181:1710-6. 2008..great progress in the characterization of alphabeta T cell differentiation, selection, and function, gammadelta T cells have remained poorly understood...
- Paracrine signaling by glial cell-derived triiodothyronine activates neuronal gene expression in the rodent brain and human cellsBeatriz C G Freitas
Laboratory of Molecular Endocrinology, Division of Endocrinology, Department of Medicine, Federal University of Sao Paulo, Sao Paulo SP, Brazil
J Clin Invest 120:2206-17. 2010..Here, we modeled TH action in the brain using an in vitro coculture system of D2-expressing H4 human glioma cells and D3-expressing SK-N-AS human neuroblastoma cells...
- Measuring the elastic properties of living cells by the atomic force microscopeManfred Radmacher
Drittes Physics Institute, Georg August Universitat, 37073 Gottingen, Germany
Methods Cell Biol 68:67-90. 2002In this chapter I discussed the possibility of measuring elastic properties of living cells by AFM...
- Mechanism for action of electromagnetic fields on cellsDimitris J Panagopoulos
Department of Cell Biology and Biophysics, Faculty of Biology, University of Athens, Panepistimiopolis, Athens GR 15784, Greece
Biochem Biophys Res Commun 298:95-102. 2002A biophysical model for the action of oscillating electric fields on cells, presented by us before [Biochem. Biophys. Res. Commun...
- EXOCYTOSIS: USING NANOSURGERY AND BIOENGINEERINGBHANU JENA; Fiscal Year: 2003..called 'pits' 8and 'depressions', have been identified and implicated in exocytosis in live pancreatic acinar cells. Our immediate research goal is to characterize and determine the involvement of these structures in the ..
- UNDERSTANDING MEMBRANE FUSION A BIOENGINEERING APPROACHBHANU JENA; Fiscal Year: 2004..Impairment of the exocytotic process in cells is involved in a number of disease states...
- UNDERSTANDING MEMBRANE FUSION A BIOENGINEERING APPROACHBHANU JENA; Fiscal Year: 2001..Impairment of the exocytotic process in cells is involved in a number of disease states...
- NUTRITIONAL CONTROL OF ASPARAGINE SYNTHETASEMichael S Kilberg; Fiscal Year: 2010..We use amino acid deprivation of cultured cells to investigate transcriptional control by nutrients...
- Probing single proteins in nanofabricated bioenvironmentDaniel Chiu; Fiscal Year: 2006..We hypothesize that the rate and mechanisms of enzymatic actions and protein interactions in vivo inside cells differ significantly from the rate and mechanisms observed in vitro inside cuvettes or other macroscopic ..
- Nutritional Regulation of Ribosomal Protein ExpressionMichael Kilberg; Fiscal Year: 2005Mammalian cells respond to amino acid deprivation by increasing the transcription of a wide variety of genes by a pathway that will be referred to as the Amino Acid Response (AAR)...
- Cancer Risks Attributable To Radiation from Pediatric CTDavid Brenner; Fiscal Year: 2006..The target audiences will be radiologists, pediatricians, family physicians, and the CT industry. We will use telephone interviews supplemented with survey questions, to aid in web content decisions. ..
- High Stability Accelerator for Sub-Cellular MicrobeamDavid Brenner; Fiscal Year: 2003..is the "front end" of our single particle I single cell microbeam which allows irradiation of individual cells and, increasingly, sub-cellular components...
- NMR detection of tumor differentiationEDWARD DELIKATNY; Fiscal Year: 2009..PB), two differentiating agents currently under Phase II clinical trials, inhibit the proliferation of cancer cells. We will concentrate on the changes in phosphatidylcholine (PtdCho) metabolites that can be observed using in vivo ..
- Profiling individual synaptic vesicles with nanofluidicsDaniel Chiu; Fiscal Year: 2003....
- NMR and NIR detection of phospholipase activity in vivoEDWARD DELIKATNY; Fiscal Year: 2004..with the differentiating agent phenylbutryate (PB) will lead to increased NIR fluorescence in the prostate tumor cells Aim 3): Demonstration of in vivo delivery and subsequent activation of phospholipases accompanying treatment of ..
- Profiling Biochemical Contents of Single MitochondriaDaniel Chiu; Fiscal Year: 2009..for generating over 90% of the cell's ATP, are implicated in aging (the site of oxidative damage in cells), and are found to play a key role in apoptosis and calcium homeostasis...
- Single-Molecule Immunoassay and DNA ScreeningEdward Yeung; Fiscal Year: 2005We will develop novel imaging technologies for real-time comprehensive analysis of molecular alterations in cells and tissues appropriate for automation and adaptation to high-throughput applications...
- RADIOLOGICAL RESEARCH ACCELERATOR FACILITY (RARAF)David Brenner; Fiscal Year: 2002..abstract_text> ..
- Nutritional Control of Transcription Factor ExpressionMichael S Kilberg; Fiscal Year: 2010Mammalian cells respond to nutritional limitation of protein/amino acids by increasing the expression of a wide spectrum of proteins via a signaling pathway that will be referred to as the Amino Acid Response (AAR)...
- Iron and Vitamin E in Childhood HIV-Associated Renal DiseasePatricio Ray; Fiscal Year: 2006..The proposed studies should have a substantial impact on the goals of the National Center for Complementary and Alternative Medicine and NIH, according to the program announcement PA-02-124. [unreadable] [unreadable]..
- 2006 Single Molecule Approaches to BiologySUNNEY XIE; Fiscal Year: 2006..unreadable] [unreadable] [unreadable]..
- Mitochondrial sites of reactive oxygen species generation in cells.Martin D Brand; Fiscal Year: 2010..the rate of cellular mitochondrial ROS generation at each specific site in the electron transport chain in cells relevant to aging, particularly neurons, muscle cells and fibroblasts...
- Regulation of Cell Signaling and AdhesionJOE RAMOS; Fiscal Year: 2006..In the PEA-15 null mouse, the most striking phenotype is a lymphoproliferative syndrome affecting both B and T cells. There is also a marked increase in hepatocyte proliferation and atrophy of the epidermis...
- MOLECULAR BASIS OF LONG CHAIN FATTY ACID TRANSPORTJean Schaffer; Fiscal Year: 2006..Mismatch between LCFA import and utilization in the pancreas, skeletal muscle, vascular endothelial cells, and the heart may play an important role in the pathogenesis of diabetes and heart failure...
- Mitochondrial Role in Manganese ToxicityThomas Gunter; Fiscal Year: 2010..It is vital that we determine the causes for its neurotoxic effects. In the cells of its target brain tissue, primarily neurons of the globus pallidus and striatum, Mn is sequestered by the ..
- Ultrafast Electron Cryomicroscopy of Single Protein ParticlesAhmed H Zewail; Fiscal Year: 2010..If successful, this advance could make structure determination to near-atomic resolution routine for large macromolecular complexes. ..
- MOLECULAR CHARACTERIZATION OF C NEOFORMANS ANTIBODIESArturo Casadevall; Fiscal Year: 2006..These studies are expected to yield new insights on fundamental aspects of antibody function as well as practical information for the development of second generation antibody reagents for human therapy. ..
- BIOENGINEERED POLYMERS FOR PARSING CELL RESPONSESLinda Griffith; Fiscal Year: 2007..has been shown to influence cell growth and migration in a broad range of cell types (fibroblasts, epithelial cells, mesenchymal stem cells) and healing situations (skin wound repair, liver regeneration, angiogenesis) We ..
- Biopolymer Surfactants for Sealing Electroporated MembranesRAPHAEL CARL LEE; Fiscal Year: 2010..Finally, we will conduct studies in parallel to determine if surfactant rescued cells manifest normal phenotype during proliferation or exhibit effects of mutations caused by unrepaired DMA.
- High Throughput Single Molecule DNA SequencingStephen Quake; Fiscal Year: 2007..99%, while reducing the cost of sequencing a mammalian genome to below $100,000. [unreadable] [unreadable]..
- Therapeutic hair follicle-derived neurospheresMeng Yang; Fiscal Year: 2007unreadable] DESCRIPTION (provided by applicant): The facile availability of pluripotent adult stem cells would have many important therapeutic applications...
- BIOLOGICAL ACTIVITY OF OXIDIZED PHOSPHOLIPIDSThomas McIntyre; Fiscal Year: 2008..to the disease process because it creates inflammatory mediators that attract monocytes, and activates vascular cells and these infiltrating monocytes...
- Novel Chimeric Molecule to Improve Islet transplantationRicardo Pastori; Fiscal Year: 2007..Such approach could minimize necessity for multiple transplants thus reducing the problem due to the shortage of cadaveric donor pancreata available for islet transplantation. [unreadable] [unreadable] [unreadable]..
- DNA Sequencing using NanoporesSteven Benner; Fiscal Year: 2007..These will then be targeted against specific sequences extracted from mammalian genomes. [unreadable] [unreadable]..
- Hepatitis HBx-Mediated PathogenesisBETTY SLAGLE; Fiscal Year: 2007..We additionally hypothesize that HBx holds nondividing cells at a G1 restriction point, and will use RNA inhibition to examine candidate proteins through which HBx may exert ..
- Polymerases for Sequencing by SynthesisSteven Benner; Fiscal Year: 2007..unreadable] [unreadable]..
- Prostate Cancer ImmunotherapyRobert Darnell; Fiscal Year: 2007..In both settings the outcomes are similar: most cells undergo growth arrest, but only few undergo apoptotic death...
- Realistic Phantom Series for OLINDA/EXM Version 2Michael Stabin; Fiscal Year: 2007..unreadable] [unreadable]..
- ECI Conference on Engineering Cell Biology II - The Cell in ContextLinda Griffith; Fiscal Year: 2007..How these principles define the architecture, organization, and function of cells and tissues in vivo will be compared to the effects of engineered substrates, biomaterials, and protein mimetics ..
- Endotoxin Generated Lipid Second MessengersThomas McIntyre; Fiscal Year: 2006..LPA stimulates PPRE-driven reporters, induces CD36 expression, and differentiates monocytes to foam cells. We show this signaling is independent of Edg (surface LPA receptors) signaling in several ways...
- Structure/function studies of RBPs in neurologic diseaseRobert Darnell; Fiscal Year: 2005..the activity of these mutants using previously established assay systems in heterologous transfected neuroblastoma cells. From these experiments, we will choose "designer" mutants for in vivo expression in mouse brain...
- CONSTRUCTION AND ANALYSIS OF RETROVIRUS MUTANTSStephen Goff; Fiscal Year: 2003..In each case, these tests will be applied to existing panels of mutants to help localize the regions needed for these activities. ..
- SYNAPTIC TRANSMISSION AND SINGLE VESICLE ANALYSISRichard Zare; Fiscal Year: 2001..abstract_text> ..
- RECEPTOR-MEDIATED ENDOCYTOSIS--MECHANISM AND FUNCTIONFREDERICK MAXFIELD; Fiscal Year: 2004..goal of this project is to understand how ligands, receptors, membrane lipids and solute molecules move through cells following endocytosis...
- STRUCTURAL BASIS--ALTERED CALCIUM HOMEOSTASIS OF AGINGTHOMAS SQUIER; Fiscal Year: 2003..age-related decreases in CaM function are responsible for the loss of calcium homeostasis observed in senescent cells. The accumulation of oxidatively modified CaM (CaMox) that is functionally inactive during aging is consistent ..
- STATISTICAL METHODS FOR CORRELATED SUBSTANCE USE DATAHeping Zhang; Fiscal Year: 2003....
- Complementation Assay for GPCR DesensitizationRICHARD EGLEN; Fiscal Year: 2003..Furthermore, these assays could have a broader role in drug discovery by identifying agonists at the large population of orphan GPCRs since stimulation of all GPCRs causes BARK and B-arrestin translocation. ..
- REGULATION OF APOPTOSIS BY VIRAL TRANSFORMING PROTEINSEileen White; Fiscal Year: 2001..The long term goal of the work is to study this important mechanism of tumor suppression. ..
- RECEPTOR AGGREGATION AND ITS EFFECTSByron Goldstein; Fiscal Year: 2002..The studies in this project are health related, bearing on allergic reactions and their treatment as well as other aspects of the immune response. ..
- RADIOBIOLOGY OF D RADIODURANS--GENETIC APPROACHMichael Daly; Fiscal Year: 2001..These studies will delineate the genetics and biochemistry of radioresistance in D. radiodurans. Insight into its strategies may have broad implications for understanding radioresistance and DSB-metabolism throughout nature. ..
- MOLECULAR TARGETS REGULATING APOPTOSISMYRTLE DAVIS; Fiscal Year: 2001..p53, bcl-2, c- jun/AP-1 and cell cycle inhibitory proteins in okadaic acid-induced apoptosis of renal epithelial cells. We have found that transformation of renal epithelial cells with ras-oncogene increases sensitivity of renal ..
- Signal Transduction by Cytokine Receptors in VivoWarner Greene; Fiscal Year: 2004..These proteins trigger similar biochemical effects within their respective target cells through receptors that have structural and functional similarities with one another...
- Calcium ATPases in Apicomplexan Parasites as Potential DL Sibley; Fiscal Year: 2006..Apicomplexan parasites rely on active invasion to enter host cells and this obligatory step is controlled in part by the regulated secretion of adhesins from micronemes...
- Trafficking of Channels Measured by EFCRICHARD EGLEN; Fiscal Year: 2004..These initial studies will form the foundation of future phase II SBIR studies to develop EFC into a HTS assay for ICC transport for use by bioteclmology and pharmaceutical companies for drug discovery. ..
- Mining slime mold for susceptibility genes to fungiArturo Casadevall; Fiscal Year: 2005..Our group has shown that C. neoformans can infect D. discoideum, replicate intracellularly, and kill the amoeboid cells. D...
- Targeted Assisted Combinational SynthesisSteven Benner; Fiscal Year: 2004..abstract_text> ..
- Tolerance versus tumor immunity in PNDRobert Darnell; Fiscal Year: 2005..These experiments offer the possibility of developing new cancer treatments and advancing the understanding of autoimmune disease of the nervous system. ..
- Comparative Morphology of Neuronal Ceroid LipofuscinosisJonathan Cooper; Fiscal Year: 2004..abstract_text> ..
- CHLORIDE CHANNEL MODULATION AND NEURONAL APOPTOSISShan Yu; Fiscal Year: 2002..Apoptotic cells undergo marked cell volume decrease...
- Dementia of Parkinson Type: Clinicopathologic PhenotypeJames Galvin; Fiscal Year: 2004..At the completion of the award period, candidate plans to develop an independent R01 project derived from the data generated by the experiments described in this proposal. ..
- COHERENT ANTI-STOKES RAMAN SCATTERING MICROSCOPYSUNNEY XIE; Fiscal Year: 2004..Scattering (CARS) microscopy to the next level: imaging of single macromolecules, biological membranes, and living cells using vibrational spectroscopy. The specific aims of the proposed studies are: 1...
- Leukocyte Responses to EndotoxinThomas McIntyre; Fiscal Year: 2005..We anticipate this work will lead to a new class of anti-inflammatory agents. ..
- Dynamic Interactions of the CytoskeletonERIKA HOLZBAUR; Fiscal Year: 2008..We have recently localized cytoplasmic dynein to the cortex of mammalian epithelial cells during interphase, where it is specifically enriched at sites of cell-cell contact...
- CaP, crystal responsible for idiopathic nephrolithasisSaeed Khan; Fiscal Year: 2008..Type density and size must conform to limits and specifications provided in the PHS 398Instructions. RESEARCH GRANT TABLE OF CONTENTS Page Numbers Face Page 1 Description, ..
- TISSUE-CRYSTAL INTERACTION CA OXALATE NEPHROLITHIASISSaeed Khan; Fiscal Year: 2003..Significantly high level of oxalate however, injures the renal epithelial cells. The injury results in promotion of crystal formation and their retention within the renal tubules by providing ..
- Probabilistic Modeling of Information from Images and Text in Online JournalsRobert Murphy; Fiscal Year: 2008..unreadable] [unreadable] [unreadable]..
- Centriole Orientation During Left/Right Symmetry Breaking in the MouseWallace Marshall; Fiscal Year: 2007..This nodal flow is created, and possibly sensed, by cilia on the node cells. How do the cilia know which way to drive the fluid? Theoretical computations as well as the investigators' ..
- Regulation of Cell Signaling and AdhesionJOE RAMOS; Fiscal Year: 2007..The hypothesis is that PEA-15 regulates the decision of T lymphocytes and some cancer cells to proliferate or to die...
- Determining Subcellular Drug DistributionsEdgar Arriaga; Fiscal Year: 2004..It is hypothesized that the subcellular localization of this drug in a few drug-treated single cells will accurately represent drug localization in the original cell culture...
- International Workshops on Iron and Copper HomeostasisMichael Garrick; Fiscal Year: 2004..The organizers face a demand for a continuation and series. Clearly one can expect the third - fifth conferences to extend the success of the previous two to more scientists and trainees and more interacting metals ..
- Small Molecule Inhibitors of CiliaWallace Marshall; Fiscal Year: 2007..Because the cells are green, this causes a dark green pellet to self-organize in the well...
- MSM Multiscale Modeling of the Heart in CardiovascularDANIEL BEARD; Fiscal Year: 2007..abstract_text> ..