nonmammalian embryo

Summary

Summary: The embryo of species other than mammals. For the chicken, use the specific CHICK EMBRYO.

Top Publications

  1. pmc p53 activation by knockdown technologies
    Mara E Robu
    University of Minnesota, Minneapolis, Minnesota, United States of America
    PLoS Genet 3:e78. 2007
  2. ncbi Analysis of genetic mosaics in developing and adult Drosophila tissues
    T Xu
    Howard Hughes Medical Institute, University of California, Berkeley 94720
    Development 117:1223-37. 1993
  3. ncbi MicroRNA expression in zebrafish embryonic development
    Erno Wienholds
    Hubrecht Laboratory, Centre for Biomedical Genetics, 3584 CT Utrecht, The Netherlands
    Science 309:310-1. 2005
  4. ncbi High-resolution in situ hybridization to whole-mount zebrafish embryos
    Christine Thisse
    Department of Cell Biology, University of Virginia Health Sciences Center, PO Box 800732, 1300 Jefferson Park Avenue, Charlottesville, Virginia 22908, USA
    Nat Protoc 3:59-69. 2008
  5. ncbi Controlling morpholino experiments: don't stop making antisense
    Judith S Eisen
    Institute of Neuroscience, 1254 University of Oregon, Eugene, OR 97403 1254, USA
    Development 135:1735-43. 2008
  6. ncbi Restricted expression of cardiac myosin genes reveals regulated aspects of heart tube assembly in zebrafish
    D Yelon
    Programs in Human Genetics and Developmental Biology, University of California, San Francisco, San Francisco, California, 94143 0448, USA
    Dev Biol 214:23-37. 1999
  7. pmc Multiple forces contribute to cell sheet morphogenesis for dorsal closure in Drosophila
    D P Kiehart
    Department of Cell Biology, Cell and Molecular Biology Program, University Program in Genetics and the Duke Comprehensive Cancer Center, Duke University Medical Center, Durham, North Carolina 27710 3709, USA
    J Cell Biol 149:471-90. 2000
  8. ncbi Cortical flows powered by asymmetrical contraction transport PAR proteins to establish and maintain anterior-posterior polarity in the early C. elegans embryo
    Edwin Munro
    Center for Cell Dynamics, Friday Harbor, WA 98250, USA
    Dev Cell 7:413-24. 2004
  9. pmc Notch promotes epithelial-mesenchymal transition during cardiac development and oncogenic transformation
    Luika A Timmerman
    University of California Comprehensive Cancer Center, San Francisco, California 94115, USA
    Genes Dev 18:99-115. 2004
  10. ncbi Silberblick/Wnt11 mediates convergent extension movements during zebrafish gastrulation
    C P Heisenberg
    Department of Anatomy and Developmental Biology, University College, London, UK
    Nature 405:76-81. 2000

Research Grants

  1. ANALYSIS OF PAIR OF RULE GENES IN SEQUENTIAL SEGMENTATIO
    DAVID WEISBLAT; Fiscal Year: 2003
  2. Spiralian developmental system for the study of multipotency
    Cesar Arenas Mena; Fiscal Year: 2008
  3. Redox-sensitive developmental pathways and gene regulatory networks
    JAMES COFFMAN; Fiscal Year: 2009
  4. Control of Cell Proliferation by Runx Proteins
    JAMES COFFMAN; Fiscal Year: 2009
  5. Bistability and Biological Oscillations
    JAMES FERRELL; Fiscal Year: 2009
  6. EARLY EVENTS IN NEURAL AND HOMEOGENETIC INDUCTION
    Claudio Stern; Fiscal Year: 2005
  7. REGULATION OF BETA-CATENIN NUCLEARIZATION
    CHARLES ETTENSOHN; Fiscal Year: 2006
  8. BIOLOGY OF BAMACAN IN TUMORIGENESIS
    Giancarlo Ghiselli; Fiscal Year: 2006
  9. Development of gonadotropin releasing hormone cells
    KATHLEEN WHITLOCK; Fiscal Year: 2009
  10. DEVELOPMENT OF THE OLFACTORY SYSTEM IN ZEBRAFISH
    KATHLEEN WHITLOCK; Fiscal Year: 2004

Detail Information

Publications339 found, 100 shown here

  1. pmc p53 activation by knockdown technologies
    Mara E Robu
    University of Minnesota, Minneapolis, Minnesota, United States of America
    PLoS Genet 3:e78. 2007
    ..p53 inhibition could potentially be applicable to other systems to suppress off-target effects caused by other knockdown technologies...
  2. ncbi Analysis of genetic mosaics in developing and adult Drosophila tissues
    T Xu
    Howard Hughes Medical Institute, University of California, Berkeley 94720
    Development 117:1223-37. 1993
    ....
  3. ncbi MicroRNA expression in zebrafish embryonic development
    Erno Wienholds
    Hubrecht Laboratory, Centre for Biomedical Genetics, 3584 CT Utrecht, The Netherlands
    Science 309:310-1. 2005
    ....
  4. ncbi High-resolution in situ hybridization to whole-mount zebrafish embryos
    Christine Thisse
    Department of Cell Biology, University of Virginia Health Sciences Center, PO Box 800732, 1300 Jefferson Park Avenue, Charlottesville, Virginia 22908, USA
    Nat Protoc 3:59-69. 2008
    ..The whole procedure takes only 3 days and, because ISH conditions are the same for each probe tested, allows high throughput analysis of zebrafish gene expression during embryogenesis...
  5. ncbi Controlling morpholino experiments: don't stop making antisense
    Judith S Eisen
    Institute of Neuroscience, 1254 University of Oregon, Eugene, OR 97403 1254, USA
    Development 135:1735-43. 2008
    ..We also discuss how the use of morpholinos can lead to misleading results, including off-target effects, and we suggest controls that will allow researchers to interpret morpholino experiments correctly...
  6. ncbi Restricted expression of cardiac myosin genes reveals regulated aspects of heart tube assembly in zebrafish
    D Yelon
    Programs in Human Genetics and Developmental Biology, University of California, San Francisco, San Francisco, California, 94143 0448, USA
    Dev Biol 214:23-37. 1999
    ..Altogether, these data suggest new models for the mechanisms that regulate the formation of a patterned heart tube and provide an important framework for future analyses of zebrafish mutants with defects in this process...
  7. pmc Multiple forces contribute to cell sheet morphogenesis for dorsal closure in Drosophila
    D P Kiehart
    Department of Cell Biology, Cell and Molecular Biology Program, University Program in Genetics and the Duke Comprehensive Cancer Center, Duke University Medical Center, Durham, North Carolina 27710 3709, USA
    J Cell Biol 149:471-90. 2000
    ..Moreover, our observations demonstrate that wound healing in this insect epidermal system parallel wound healing in vertebrate tissues in situ and vertebrate cells in culture (for review see Kiehart, D.P. 1999. Curr. Biol. 9:R602-R605)...
  8. ncbi Cortical flows powered by asymmetrical contraction transport PAR proteins to establish and maintain anterior-posterior polarity in the early C. elegans embryo
    Edwin Munro
    Center for Cell Dynamics, Friday Harbor, WA 98250, USA
    Dev Cell 7:413-24. 2004
    ..Similar actomyosin flows accompany the establishment of PAR asymmetries that form after the one-cell stage, suggesting that actomyosin-mediated cortical flows have a general role in PAR asymmetry...
  9. pmc Notch promotes epithelial-mesenchymal transition during cardiac development and oncogenic transformation
    Luika A Timmerman
    University of California Comprehensive Cancer Center, San Francisco, California 94115, USA
    Genes Dev 18:99-115. 2004
    ..Notch is expressed in embryonic regions where EMT occurs, suggesting an intimate and fundamental role for Notch, which may be reactivated during tumor metastasis...
  10. ncbi Silberblick/Wnt11 mediates convergent extension movements during zebrafish gastrulation
    C P Heisenberg
    Department of Anatomy and Developmental Biology, University College, London, UK
    Nature 405:76-81. 2000
    ..Our results provide genetic and experimental evidence that Wnt activity in lateral tissues has a crucial role in driving the convergent extension movements underlying vertebrate gastrulation...
  11. ncbi Asymmetric distribution of numb protein during division of the sensory organ precursor cell confers distinct fates to daughter cells
    M S Rhyu
    Howard Hughes Medical Institute, University of California, San Francisco 94143 0724
    Cell 76:477-91. 1994
    ..Thus, numb functions to determine the fates of the secondary precursors; the differential distribution of numb as the SOP divides generates an asymmetric division in which the daughter cells acquire distinct identities...
  12. pmc Live imaging of wound inflammation in Drosophila embryos reveals key roles for small GTPases during in vivo cell migration
    Brian Stramer
    Department of Physiology, School of Medical Sciences, University of Bristol, Bristol, BS8 1TD, UK
    J Cell Biol 168:567-73. 2005
    ..Cdc42 is necessary for maintaining cellular polarity and yet, despite in vitro evidence, is dispensable for sensing and crawling toward wound cues...
  13. pmc Zebrafish miR-214 modulates Hedgehog signaling to specify muscle cell fate
    Alex S Flynt
    Department of Biological Sciences, Vanderbilt University, Nashville, Tennessee 37235, USA
    Nat Genet 39:259-63. 2007
    ..We show that su(fu) mRNA, encoding a negative regulator of Hedgehog signaling, is targeted by miR-214. Through regulation of su(fu), miR-214 enables precise specification of muscle cell types by sharpening cellular responses to Hedgehog...
  14. ncbi sonic hedgehog and vascular endothelial growth factor act upstream of the Notch pathway during arterial endothelial differentiation
    Nathan D Lawson
    Laboratory of Molecular Genetics, NICHD, NIH, Bethesda, MD 20892, USA
    Dev Cell 3:127-36. 2002
    ..These studies reveal a complex signaling cascade responsible for establishing arterial cell fate and suggest differential effects of Vegf on developing endothelial cells...
  15. ncbi Cellular and molecular analyses of vascular tube and lumen formation in zebrafish
    Suk Won Jin
    Department of Biochemistry and Biophysics and Cardiovascular Research Institute, Program in Developmental Biology, University of California San Francisco, 1550 Fourth Street, San Francisco, CA 94143, USA
    Development 132:5199-209. 2005
    ..These studies provide the tools and a cellular framework for the investigation of mutations affecting vasculogenesis in zebrafish...
  16. ncbi Patterned gene expression directs bipolar planar polarity in Drosophila
    Jennifer A Zallen
    Department of Molecular Biology, Princeton University, Lewis Thomas Lab, Washington Road, Princeton, NJ 08544, USA
    Dev Cell 6:343-55. 2004
    ..These results indicate that spatial patterns of gene expression coordinate planar polarity across a multicellular population through the localized distribution of proteins required for cell movement...
  17. ncbi Vertebrate Smoothened functions at the primary cilium
    Kevin C Corbit
    Developmental and Stem Cell Biology Program, University of California, San Francisco, California 94143, USA
    Nature 437:1018-21. 2005
    ..Thus, Hh-dependent translocation to cilia is essential for Smo activity, suggesting that Smo acts at the primary cilium...
  18. ncbi Insertional mutagenesis in zebrafish rapidly identifies genes essential for early vertebrate development
    Gregory Golling
    Center for Cancer Research and Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA
    Nat Genet 31:135-40. 2002
    ....
  19. ncbi Morphologic and functional characterization of granulocytes and macrophages in embryonic and adult zebrafish
    G J Lieschke
    Ludwig Institute for Cancer Research, The Royal Melbourne Hospital, Parkville, Victoria, Australia
    Blood 98:3087-96. 2001
    ..These observations will facilitate the application of this genetically tractable organism to the study of myelopoiesis...
  20. ncbi Lithium perturbation and goosecoid expression identify a dorsal specification pathway in the pregastrula zebrafish
    S E Stachel
    Department of Human Genetics, University of Utah, Salt Lake City 84112
    Development 117:1261-74. 1993
    ..Our results demonstrate that the zebrafish dorsal axis is signaled by a pathway initiated in the cleavage-stage embryo. Furthermore, they provide novel insights into anterior morphogenesis...
  21. ncbi Control of chemokine-guided cell migration by ligand sequestration
    Bijan Boldajipour
    Center for Molecular Biology of Inflammation, Von Esmarch Strasse 56, D 48149 Munster, Germany
    Cell 132:463-73. 2008
    ....
  22. pmc Loss of function of def selectively up-regulates Delta113p53 expression to arrest expansion growth of digestive organs in zebrafish
    Jun Chen
    Laboratory of Functional Genomics, Laboratory of Molecular and Developmental Immunology, Laboratory of Control of p53 Pathway, Institute of Molecular and Cell Biology, Proteos, Singapore 138673
    Genes Dev 19:2900-11. 2005
    ....
  23. pmc Cytoplasmic dynein is required for distinct aspects of MTOC positioning, including centrosome separation, in the one cell stage Caenorhabditis elegans embryo
    P GONCZY
    European Molecular Biology Laboratory, Heidelberg, D 69117 Germany
    J Cell Biol 147:135-50. 1999
    ..In conjunction with our observation of cytoplasmic dynein distribution at the periphery of nuclei, these results lead us to propose a mechanism in which cytoplasmic dynein anchored on the nucleus drives centrosome separation...
  24. ncbi Guidance of primordial germ cell migration by the chemokine SDF-1
    Maria Doitsidou
    Germ Cell Development, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077, Gottingen, Germany
    Cell 111:647-59. 2002
    ..Finally, we show that the PGCs can be attracted toward an ectopic source of the chemokine, strongly suggesting that this molecule provides a key directional cue for the PGCs...
  25. pmc Regulation of neurogenesis by interkinetic nuclear migration through an apical-basal notch gradient
    Filippo Del Bene
    Department of Physiology, Programs in Neuroscience, Genetics, and Developmental Biology, University of California San Francisco, San Francisco, CA 94158 2722, USA
    Cell 134:1055-65. 2008
    ..Our data indicate that the function of INM is to balance the exposure of progenitor nuclei to neurogenic versus proliferative signals...
  26. ncbi Zebrafish as a model organism for the identification and characterization of drugs and genes affecting p53 signaling
    Ulrike Langheinrich
    Exelixis Deutschland GmbH, Spemannstrasse 35, D 72076 Tubingen, Germany
    Curr Biol 12:2023-8. 2002
    ..We conclude that zebrafish represents a promising model organism for future compound-based and genetic screens and believe that it will help to identify and characterize new anticancer drugs and new targets for cancer treatment...
  27. ncbi Sequential protein recruitment in C. elegans centriole formation
    Marie Delattre
    Swiss Institute for Experimental Cancer Research, School of Life Sciences ISREC, Swiss Federal Institute of Technology EPFL, Lausanne, Switzerland
    Curr Biol 16:1844-9. 2006
    ..Several of these proteins have homologs in other metazoans, and we expect the assembly pathway that stems from our work to be conserved...
  28. pmc Polarized insertion of new membrane from a cytoplasmic reservoir during cleavage of the Drosophila embryo
    T Lecuit
    Howard Hughes Medical Institute, Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA
    J Cell Biol 150:849-60. 2000
    ..We thus identify a tightly regulated scheme of polarized membrane insertion during cellularization. We propose that such a mechanism could participate in the progressive emergence of apical-basal polarity...
  29. ncbi Initiation of Wnt signaling: control of Wnt coreceptor Lrp6 phosphorylation/activation via frizzled, dishevelled and axin functions
    Xin Zeng
    The F M Kirby Neurobiology Center, Children s Hospital Boston, Department of Neurology, Harvard Medical School, Boston, MA 02115, USA
    Development 135:367-75. 2008
    ..We discuss the dual roles of the axin-Gsk3 complex and signal amplification by Lrp6-axin interaction during Wnt/beta-catenin signaling...
  30. ncbi The expression pattern of the Drosophila vesicular glutamate transporter: a marker protein for motoneurons and glutamatergic centers in the brain
    Andrea Mahr
    Max Planck Institut fur Entwicklungsbiologie, Abt III Genetik, Spemannstr 35, 72076 Tubingen, Germany
    Gene Expr Patterns 6:299-309. 2006
    ..Given that vesicular glutamate transporters are essential for the uptake of the neurotransmitter glutamate into synaptic vesicles these tools provide a means to test gene function in these functionally important neurons...
  31. ncbi Fgf8 morphogen gradient forms by a source-sink mechanism with freely diffusing molecules
    Shuizi Rachel Yu
    Developmental Genetics, Biotechnology Center, TUD, Tatzberg 47 49, 01307 Dresden, Germany
    Nature 461:533-6. 2009
    ..Our results demonstrate that a freely diffusing morphogen can set up concentration gradients in a complex multicellular tissue by a simple source-sink mechanism...
  32. ncbi Drosophila melanogaster embryonic haemocytes: masters of multitasking
    Will Wood
    Department of Biology and Biochemistry, University of Bath, BA2 7AY, UK
    Nat Rev Mol Cell Biol 8:542-51. 2007
    ..Instead, throughout development, embryonic haemocytes are occupied with other tasks: they undergo complex migrations and carry out several non-immune functions that are crucial for successful embryogenesis...
  33. ncbi A role for Piwi and piRNAs in germ cell maintenance and transposon silencing in Zebrafish
    Saskia Houwing
    Hubrecht Laboratory, Uppsalalaan 8, Utrecht, Netherlands
    Cell 129:69-82. 2007
    ..Furthermore, we show that piRNAs are Dicer independent and that their 3' end likely carries a 2'O-Methyl modification...
  34. ncbi The transcription factors Scl and Lmo2 act together during development of the hemangioblast in zebrafish
    Lucy J Patterson
    Weatherall Institute of Molecular Medicine, Oxford University, John Radcliffe Hospital, Headington, Oxford, United Kingdom
    Blood 109:2389-98. 2007
    ..Thus, expression of these critical regulators is dependent on continued expression of upstream regulators, which may include cell-extrinsic signals...
  35. pmc Proteomics of early zebrafish embryos
    Vinzenz Link
    Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauer Str 108, 01307 Dresden, Germany
    BMC Dev Biol 6:1. 2006
    ..While genetic methods are readily available in zebrafish, protocols for two dimensional (2D) gel electrophoresis and proteomics have yet to be developed...
  36. ncbi The cytoskeleton and morphogenesis of the early Drosophila embryo
    W Sullivan
    Department of Biology, Sinsheimer Laboratories, University of California, Santa Cruz 95064, USA
    Curr Opin Cell Biol 7:18-22. 1995
    ....
  37. pmc RNA polymerase stalling at developmental control genes in the Drosophila melanogaster embryo
    Julia Zeitlinger
    Whitehead Institute for Biomedical Research, Nine Cambridge Center, Cambridge, Massachusetts 02142, USA
    Nat Genet 39:1512-6. 2007
    ..We propose that Pol II stalling facilitates rapid temporal and spatial changes in gene activity during development...
  38. ncbi FGF-dependent mechanosensory organ patterning in zebrafish
    Alex Nechiporuk
    University of Washington, School of Medicine, Department of Biological Structure, Seattle, WA 98195 7420, USA
    Science 320:1774-7. 2008
    ..This previously unrecognized mechanism may be applicable to understanding segmentation and morphogenesis in other organ systems...
  39. ncbi The TAGteam DNA motif controls the timing of Drosophila pre-blastoderm transcription
    John R ten Bosch
    Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720 3204, USA
    Development 133:1967-77. 2006
    ....
  40. ncbi Zebrafish embryos as a model host for the real time analysis of Salmonella typhimurium infections
    Astrid M van der Sar
    Department of Medical Microbiology, Vrije Universiteit Medical Centre, Van der Boechorststraat 7, 1081 BT Amsterdam, The Netherlands
    Cell Microbiol 5:601-11. 2003
    ..In conclusion, the zebrafish embryo model allows for rapid, non-invasive and real time analysis of bacterial infections in a vertebrate host...
  41. pmc Reassessing the role and dynamics of nonmuscle myosin II during furrow formation in early Drosophila embryos
    Anne Royou
    CNRS Centre de Génétique Moléculaire, 91198 Gif sur Yvette, France
    Mol Biol Cell 15:838-50. 2004
    ..We suggest that cellularization can be divided into two distinct processes: furrow ingression, driven by microtubule mediated vesicle delivery, and basal closure, which is mediated by actin/myosin based constriction...
  42. ncbi Transgenesis and gene trap methods in zebrafish by using the Tol2 transposable element
    Koichi Kawakami
    Division of Molecular and Developmental Biology, National Institute of Genetics, Mishima, Shizuoka 411 8540, Japan
    Methods Cell Biol 77:201-22. 2004
  43. pmc Zebrafish vasa RNA but not its protein is a component of the germ plasm and segregates asymmetrically before germline specification
    H Knaut
    Max Planck Institut für Entwicklungsbiologie Abteilung Genetik, 72076 Tubingen, Germany
    J Cell Biol 149:875-88. 2000
    ..Triggered by maternal signals, the pattern of germ plasm segregation changes, which results in the expression of primordial germ cell-specific genes such as vasa and, consequently, in germline fate commitment...
  44. ncbi A morphogen gradient of Wnt/beta-catenin signalling regulates anteroposterior neural patterning in Xenopus
    C Kiecker
    Division of Molecular Embryology, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, 69120 Heidelberg, Germany
    Development 128:4189-201. 2001
    ..Our results indicate that an activity gradient of Wnt/beta-catenin signalling acts as transforming morphogen to pattern the Xenopus central nervous system...
  45. ncbi The PDGF/VEGF receptor controls blood cell survival in Drosophila
    Katja Brückner
    Department of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA
    Dev Cell 7:73-84. 2004
    ..This function of PVR shows striking conservation with mammalian hematopoiesis and establishes Drosophila as a model to study hematopoietic cell survival in development and disease...
  46. ncbi Zebrafish MiR-430 promotes deadenylation and clearance of maternal mRNAs
    Antonio J Giraldez
    Developmental Genetics Program, Skirball Institute of Biomolecular Medicine, and Department of Cell Biology, New York University School of Medicine, New York, NY 10016, USA
    Science 312:75-9. 2006
    ..We also show that miR-430 accelerates the deadenylation of target mRNAs. These results suggest that miR-430 facilitates the deadenylation and clearance of maternal mRNAs during early embryogenesis...
  47. pmc Distinct mechanisms regulate hemocyte chemotaxis during development and wound healing in Drosophila melanogaster
    Will Wood
    Instituto Gulbenkian de Ciencia, 2780 156 Oeiras, Portugal
    J Cell Biol 173:405-16. 2006
    ....
  48. ncbi Expression of the zebrafish paired box gene pax[zf-b] during early neurogenesis
    S Krauss
    Department of Microbiology, University of Tromsø, Norway
    Development 113:1193-206. 1991
    ..The characteristic expression pattern of pax[zf-b] in the neural tube suggests an involvement of this gene in the regionalization of the midbrain as well as in the specification of neuronal cell fates at early embryonic stages...
  49. ncbi Convergent extension: the molecular control of polarized cell movement during embryonic development
    John B Wallingford
    Department of Molecular and Cell Biology, 401 Barker Hall, University of California, Berkeley, 94720, USA
    Dev Cell 2:695-706. 2002
    ..Focusing on frogs and fish, we review the different cellular mechanisms and the well-conserved signaling pathways that underlie this process...
  50. ncbi Dynamic Fgf signaling couples morphogenesis and migration in the zebrafish lateral line primordium
    Virginie Lecaudey
    European Molecular Biology Laboratory, Meyerhofstrasse 1, Heidelberg, Germany
    Development 135:2695-705. 2008
    ..Furthermore, this work uncovers a surprising link between internal tissue organization and collective migration...
  51. ncbi Atypical protein kinase C cooperates with PAR-3 to establish embryonic polarity in Caenorhabditis elegans
    Y Tabuse
    Fundamental Research Laboratories, NEC Corporation, Tsukuba, Ibaraki 305, Japan
    Development 125:3607-14. 1998
    ..We conclude that PKC-3 plays an indispensable role in establishing embryonic polarity through interaction with PAR-3...
  52. ncbi An orthologue of the kit-related gene fms is required for development of neural crest-derived xanthophores and a subpopulation of adult melanocytes in the zebrafish, Danio rerio
    D M Parichy
    Department of Genetics, Washington University Medical School, St Louis, MO 63110, USA
    Development 127:3031-44. 2000
    ..These findings reveal an unexpected role for fms in pigment pattern development and demonstrate that parallel neural crest-derived pigment cell populations depend on the activities of two essentially paralogous genes, kit and fms...
  53. pmc Loss of myotubularin function results in T-tubule disorganization in zebrafish and human myotubular myopathy
    James J Dowling
    Department of Pediatrics, University of Michigan Medical Center, Ann Arbor, Michigan, USA
    PLoS Genet 5:e1000372. 2009
    ..Based on our findings, we speculate that congenital myopathies, usually considered entities with similar clinical features but very disparate pathomechanisms, may at their root be disorders of calcium homeostasis...
  54. pmc MEI-1/MEI-2 katanin-like microtubule severing activity is required for Caenorhabditis elegans meiosis
    M Srayko
    Department of Biochemistry, Development Research Group, University of Calgary, Calgary, Alberta, T2N 4N1 Canada
    Genes Dev 14:1072-84. 2000
    ..These data lead us to conclude that MEI-1/MEI-2 microtubule-severing activity is required for meiotic spindle organization in C. elegans...
  55. ncbi Control of embryonic spindle positioning and Galpha activity by C. elegans RIC-8
    Claudia Couwenbergs
    Institute of Biochemistry, ETH Eidgenössische Technische Hochschule Hoenggerberg, 8093 Zurich, Switzerland
    Curr Biol 14:1871-6. 2004
    ..We suggest that RIC-8 directly modulates Galpha activity and that Galpha-GTP is the signaling molecule regulating spindle positioning in the early embryo...
  56. ncbi Unique and conserved aspects of gut development in zebrafish
    Kenneth N Wallace
    Department of Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Dev Biol 255:12-29. 2003
    ....
  57. pmc CHD1 motor protein is required for deposition of histone variant H3.3 into chromatin in vivo
    Alexander Y Konev
    Department of Cell Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    Science 317:1087-90. 2007
    ..Furthermore, our results point to the general requirement of adenosine triphosphate (ATP)-utilizing motor proteins for histone deposition in vivo...
  58. pmc CAR-1, a protein that localizes with the mRNA decapping component DCAP-1, is required for cytokinesis and ER organization in Caenorhabditis elegans embryos
    Jayne M Squirrell
    Laboratory for Molecular Biology, University of Wisconsin, Madison, WI 53706, USA
    Mol Biol Cell 17:336-44. 2006
    ..Thus, CAR-1, a protein likely to be associated with RNA metabolism, plays an essential role in the late stage of cytokinesis, suggesting a novel link between RNA, membrane trafficking and cytokinesis in the C. elegans embryo...
  59. ncbi Trafficking through Rab11 endosomes is required for cellularization during Drosophila embryogenesis
    Anne Pelissier
    Laboratoire de Genetique et de Physiologie du Developpement, Institut de Biologie du Developpement de Marseille, France
    Curr Biol 13:1848-57. 2003
    ..In Drosophila, the process is specialized and called cellularization. The trafficking pathways that underlie this process and that are responsible for the mobilization of membrane pools, however, remain poorly understood...
  60. pmc The anterior determinant bicoid of Drosophila is a derived Hox class 3 gene
    M Stauber
    Max Planck Institut fur biophysikalische Chemie, Abteilung Molekulare Entwicklungsbiologie, Am Fassberg 11, D 37077 Gottingen, Germany
    Proc Natl Acad Sci U S A 96:3786-9. 1999
    ....
  61. ncbi Expression of a type II collagen gene in the zebrafish embryonic axis
    Y L Yan
    Institute of Neuroscience, University of Oregon, Eugene 97403, USA
    Dev Dyn 203:363-76. 1995
    ....
  62. pmc Drosophila atypical protein kinase C associates with Bazooka and controls polarity of epithelia and neuroblasts
    A Wodarz
    Institut fur Genetik, Heinrich Heine Universitat Dusseldorf, 40225 Dusseldorf, Germany
    J Cell Biol 150:1361-74. 2000
    ..This study is the first functional analysis of an atypical protein kinase C isoform using a loss-of-function allele in a genetically tractable organism...
  63. ncbi Zebrafish pea3 and erm are general targets of FGF8 signaling
    H Roehl
    Max Planck Institut fur Entwicklungsbiologie, Spemannstrasse 35 III, D 72076, Tubingen, Germany
    Curr Biol 11:503-7. 2001
    ..To test whether this differential expression is established by FGF8, we have induced FGF8 ectopically and show that it is sufficient to recapitulate the endogenous nested expression pattern of pea3 and erm...
  64. ncbi Mechanisms of asymmetric cell division: flies and worms pave the way
    PIERRE GONCZY
    Swiss Institute for Experimental Cancer Research ISREC, Swiss Federal Institute of Technology EPFL, School of Life Sciences, Lausanne, Switzerland
    Nat Rev Mol Cell Biol 9:355-66. 2008
    ..Lessons learned from these two model organisms also apply to cells that divide asymmetrically in other metazoans, such as self-renewing stem cells in mammals...
  65. ncbi Drosophila checkpoint kinase 2 couples centrosome function and spindle assembly to genomic integrity
    Saeko Takada
    Program in Molecular Medicine, University of Massachusetts Medical School, 373 Plantation Street, Worcester, MA 01605, USA
    Cell 113:87-99. 2003
    ..DmChk2 is therefore essential for a "mitotic catastrophe" signal that disrupts centrosome function in response to genotoxic stress and ensures that mutant and aneuploid nuclei are eliminated from the embryonic precursor pool...
  66. ncbi The Drosophila gastrulation gene concertina encodes a G alpha-like protein
    S Parks
    Department of Molecular Biology, Princeton University, New Jersey 08540
    Cell 64:447-58. 1991
    ..The phenotype of embryos from homozygous cta mothers suggests that the cta gene plays a role in a signal transduction pathway used during gastrulation...
  67. pmc Zebrafish blowout provides genetic evidence for Patched1-mediated negative regulation of Hedgehog signaling within the proximal optic vesicle of the vertebrate eye
    Jiwoon Lee
    Section of Molecular Cell and Developmental Biology and Institute for Cell and Molecular Biology, USA
    Dev Biol 319:10-22. 2008
    ....
  68. ncbi Organization and function of the blood-brain barrier in Drosophila
    Tobias Stork
    Institut fur Neurobiologie, Universitat Munster, D 48149 Münster Germany
    J Neurosci 28:587-97. 2008
    ....
  69. ncbi Distinct roles for Fgf, Wnt and retinoic acid in posteriorizing the neural ectoderm
    Tetsuhiro Kudoh
    Department of Anatomy and Developmental Biology, University College London, Gower St, London WC1E 6BT, UK
    Development 129:4335-46. 2002
    ..Thus, cyp26 has an important role in linking the Fgf, Wnt and RA signals to regulate AP patterning of the neural ectoderm in the late blastula to gastrula embryo in zebrafish...
  70. pmc AceTree: a tool for visual analysis of Caenorhabditis elegans embryogenesis
    Thomas J Boyle
    Department of Genome Sciences, University of Washington, Seattle, WA 98195, USA
    BMC Bioinformatics 7:275. 2006
    ....
  71. ncbi Noggin1 and Follistatin-like2 function redundantly to Chordin to antagonize BMP activity
    Sophie Dal-Pra
    Institut de Genetique et de Biologie Moleculaire et Cellulaire, UMR 7104, CNRS INSERM ULP, 1 rue Laurent Fries, BP 10142, CU de Strasbourg, 67404 Illkirch Cedex, France
    Dev Biol 298:514-26. 2006
    ....
  72. ncbi Hedgehog signaling is required for adult blood stem cell formation in zebrafish embryos
    Martin Gering
    Institute of Genetics, University of Nottingham, Queen s Medical Centre, Nottingham, NG7 2UH, United Kingdom
    Dev Cell 8:389-400. 2005
    ..Furthermore, HSC and DA formation also share Vegf and Notch requirements, which further distinguishes them from primitive hematopoiesis and underlines their close relationship during vertebrate development...
  73. pmc Pcl-PRC2 is needed to generate high levels of H3-K27 trimethylation at Polycomb target genes
    Maxim Nekrasov
    Gene Expression Programme, EMBL, Heidelberg, Germany
    EMBO J 26:4078-88. 2007
    ..Pcl-PRC2 is thus the HMTase that generates the high levels of H3-K27 trimethylation in Polycomb target genes that are needed to maintain a Polycomb-repressed chromatin state...
  74. pmc Gastrulation in Drosophila: the logic and the cellular mechanisms
    M Leptin
    Institut fur Genetik, Universitat zu Koln, Weyertal 121, D 50931 Köln, Germany
    EMBO J 18:3187-92. 1999
    ....
  75. pmc Sepsid even-skipped enhancers are functionally conserved in Drosophila despite lack of sequence conservation
    Emily E Hare
    Department of Molecular and Cell Biology, University of California Berkeley, Berkeley, California, United States of America
    PLoS Genet 4:e1000106. 2008
    ..Together, these observations suggest that the local arrangement of binding sites relative to each other is more important than their overall arrangement into larger units of cis-regulatory function...
  76. ncbi Directional migration of neural crest cells in vivo is regulated by Syndecan-4/Rac1 and non-canonical Wnt signaling/RhoA
    Helen K Matthews
    Department of Anatomy and Development Biology, University College London, Gower Street, London WC1E 6BT, UK
    Development 135:1771-80. 2008
    ..In addition, we show that RhoA inhibits Rac in NC cells. We present a model in which Syn4 and PCP control directional NC migration by, at least in part, regulating membrane protrusions through the regulation of small GTPase activities...
  77. ncbi Fgf10 regulates hepatopancreatic ductal system patterning and differentiation
    P Duc Si Dong
    Department of Biochemistry and Biophysics, Programs in Developmental Biology, Genetics and Human Genetics, and the Diabetes Center, University of California, San Francisco, 1550 Fourth Street, San Francisco, California 94158, USA
    Nat Genet 39:397-402. 2007
    ..These data shed light onto how the multipotent cells of the foregut endoderm, and subsequently those of the hepatopancreatic duct, are directed toward different organ fates...
  78. ncbi Multicellular rosette formation links planar cell polarity to tissue morphogenesis
    J Todd Blankenship
    Developmental Biology Program, Sloan Kettering Institute, New York, New York 10021, USA
    Dev Cell 11:459-70. 2006
    ..We propose that the generation of higher-order rosette structures links local cell interactions to global tissue reorganization during morphogenesis...
  79. ncbi Pax6 induces ectopic eyes in a vertebrate
    R L Chow
    Developmental Genetics Program, Skirball Institute of Biomolecular Medicine, New York University Medical Center, New York, NY 10016, USA
    Development 126:4213-22. 1999
    ..These data show that in vertebrates, as in the invertebrate Drosophila melanogaster, Pax6 is both necessary and sufficient to trigger the cascade of events required for eye formation...
  80. pmc The chromokinesin, KLP3A, dives mitotic spindle pole separation during prometaphase and anaphase and facilitates chromatid motility
    Mijung Kwon
    University of California Davis, Davis, California 95616, USA
    Mol Biol Cell 15:219-33. 2004
    ..We propose that KLP3A acts on MTs associated with chromosome arms and the central spindle to organize ipMT bundles, to drive spindle pole separation and to facilitate chromatid motility...
  81. ncbi Zebrafish Rho kinase 2 acts downstream of Wnt11 to mediate cell polarity and effective convergence and extension movements
    Florence Marlow
    Department of Biological Sciences, Vanderbilt University, VU Station B 351634, Nashville, TN 37235 1634, USA
    Curr Biol 12:876-84. 2002
    ..Moreover, how defective mediolateral cell polarity impacts CE is not understood...
  82. pmc Efficient transposition of the Tol2 transposable element from a single-copy donor in zebrafish
    Akihiro Urasaki
    Division of Molecular and Developmental Biology, National Institute of Genetics, and Department of Genetics, Graduate University for Advanced Studies SOKENDAI, Mishima, Shizuoka 411 8540, Japan
    Proc Natl Acad Sci U S A 105:19827-32. 2008
    ..Our present study demonstrates that the in vivo Tol2 transposition system is useful for creating genome-wide insertions from a single-copy donor and should facilitate functional genomics and transposon biology in vertebrates...
  83. ncbi Presynaptic development at the Drosophila neuromuscular junction: assembly and localization of presynaptic active zones
    A Prokop
    Department of Zoology, University of Cambridge, United Kingdom
    Neuron 17:617-26. 1996
    ..Thus, the process of synapse formation can be genetically separated from the process of target recognition, revealing that localization of presynaptic active zones requires mef2-dependent muscle differentiation...
  84. ncbi Characterization of a notochord-specific enhancer from the Brachyury promoter region of the ascidian, Ciona intestinalis
    J C Corbo
    Department of Biology, University of California, San Diego, La Jolla 92093 0347, USA
    Development 124:589-602. 1997
    ..We discuss the implications of this analysis with regard to the evolutionary conservation of integrative enhancers, and the subdivision of the axial and paraxial mesoderm in vertebrates...
  85. ncbi A role for FGF-8 in the dorsoventral patterning of the zebrafish gastrula
    M Fürthauer
    Institut de Genetique et de Biologie Moleculaire et Cellulaire, CNRS, INSERM, ULP, Illkirch, France
    Development 124:4253-64. 1997
    ..We conclude that FGF-8 is involved in defining dorsoventral identity and is an important organizing factor responsible for specification of mesodermal and ectodermal dorsolateral territories of the zebrafish gastrula...
  86. pmc FoxH1 negatively modulates flk1 gene expression and vascular formation in zebrafish
    Jayoung Choi
    Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA 90095, USA
    Dev Biol 304:735-44. 2007
    ..Taken together, our data provide the first evidence that flk1 is a direct target of FoxH1 and that FoxH1 is involved in vessel formation in zebrafish...
  87. pmc Formation of the bicoid morphogen gradient: an mRNA gradient dictates the protein gradient
    Alexander Spirov
    Center for Developmental Genetics, Stony Brook University, Stony Brook, NY 11794 5140, USA
    Development 136:605-14. 2009
    ....
  88. ncbi Silencing of Smed-betacatenin1 generates radial-like hypercephalized planarians
    Marta Iglesias
    Departament de Genetica, Universitat de Barcelona, 08028 Barcelona, Spain
    Development 135:1215-21. 2008
    ..mediterranea beta-catenins could be functionally specialized and that only Smed-betacatenin1 is involved in Wnt signalling...
  89. ncbi p53-dependent neuronal cell death in a DJ-1-deficient zebrafish model of Parkinson's disease
    Sandrine Bretaud
    Centre for Developmental and Biomedical Genetics, University of Sheffield, Sheffield, UK
    J Neurochem 100:1626-35. 2007
    ..Our study demonstrates the utility of zebrafish as a new animal model to study PD gene defects and suggests that modulation of downstream mechanisms, such as p53 inhibition, may be of therapeutic benefit...
  90. pmc Dynamic analysis of filopodial interactions during the zippering phase of Drosophila dorsal closure
    Thomas H Millard
    Department of Biochemistry, School of Medical Sciences, University of Bristol, University Walk, Bristol BS8 1DA, UK
    Development 135:621-6. 2008
    ..Finally, we characterise the behaviour of a patched-expressing cell that we observe within the engrailed region of segments A1-A5, and provide evidence that this cell contributes to cell matching...
  91. ncbi Cranio-lenticulo-sutural dysplasia is caused by a SEC23A mutation leading to abnormal endoplasmic-reticulum-to-Golgi trafficking
    Simeon A Boyadjiev
    McKusick Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Nat Genet 38:1192-7. 2006
    ..Our observations suggest that disrupted endoplasmic reticulum export of the secretory proteins required for normal morphogenesis accounts for CLSD...
  92. ncbi Segmentation
    Diethard Tautz
    Institut für Genetik der Universität zu Köln, Weyertal 121, 50931, Germany
    Dev Cell 7:301-12. 2004
    ..An old concept of comparative anatomy, the enterocoele theory, is compatible with a single origin of segmentation mechanisms and could therefore provide a conceptual framework for assessing these molecular similarities...
  93. pmc Quantitative transcript imaging in normal and heat-shocked Drosophila embryos by using high-density oligonucleotide arrays
    R Leemans
    Institute of Zoology, Biocenter Pharmacenter, University of Basel, CH 4056 Basel, Switzerland
    Proc Natl Acad Sci U S A 97:12138-43. 2000
    ..These results demonstrate that high-density oligonucleotide arrays are sensitive, efficient, and quantitative instruments for the analysis of large scale gene expression in Drosophila embryos...
  94. pmc Dishevelled controls apical docking and planar polarization of basal bodies in ciliated epithelial cells
    Tae Joo Park
    Department of Molecular Cell and Developmental Biology and Institute for Cellular and Molecular Biology, University of Texas, Austin, Texas 78712, USA
    Nat Genet 40:871-9. 2008
    ..These results demonstrate previously undescribed functions for PCP signaling components and suggest that a common signaling apparatus governs both apical docking and planar polarization of basal bodies...
  95. pmc How the Dorsal gradient works: insights from postgenome technologies
    Joung Woo Hong
    Division of Genetics, Genomics, and Development, Department of Molecular and Cell Biology, Center for Integrative Genomics, University of California, Berkeley, CA 94720, USA
    Proc Natl Acad Sci U S A 105:20072-6. 2008
    ..The identification of 60-70 Dorsal target genes, along with the characterization of approximately 35 associated regulatory DNAs, suggests that there are at least six different regulatory codes driving diverse DV expression profiles...
  96. ncbi Neurodevelopmental defects in zebrafish (Danio rerio) at environmentally relevant dioxin (TCDD) concentrations
    Adrian Hill
    Laboratory for Environmental Gene Regulation, School of Biological Sciences, Biosciences Building, University of Liverpool, Crown Street, Liverpool Merseyside L69 7ZB, United Kingdom
    Toxicol Sci 76:392-9. 2003
    ..This disruption of neuronal development provides the basis for understanding the neurotoxic effects of these compounds...
  97. pmc The A- and B-type cyclins of Drosophila are accumulated and destroyed in temporally distinct events that define separable phases of the G2-M transition
    W G Whitfield
    Department of Biochemistry, The University, Dundee, Scotland
    EMBO J 9:2563-72. 1990
    ..These observations suggest that cyclin A has a role in the G2-M transition that is independent of spindle formation, and that entry into anaphase is a key requirement for the degradation of cyclin B...
  98. ncbi Whole-somite rotation generates muscle progenitor cell compartments in the developing zebrafish embryo
    Georgina E Hollway
    The Victor Chang Cardiac Research Institute, 384 Victoria Street, Darlinghurst, New South Wales 2010, Australia
    Dev Cell 12:207-19. 2007
    ..We also show that formation of this compartment occurs via whole-somite rotation, a process that requires the action of the Sdf family of secreted cytokines...
  99. pmc The role of the segmentation gene hairy in Tribolium
    Manuel Aranda
    Department of Genetics, University of Cologne, Zülpicherstrasse 47, 50674, Koln, Germany
    Dev Genes Evol 218:465-77. 2008
    ..Our results imply that there is considerable divergence in hairy function between Tribolium and Drosophila...
  100. ncbi Comm function in commissural axon guidance: cell-autonomous sorting of Robo in vivo
    Krystyna Keleman
    Institute of Molecular Biotechnology of the Austrian Academy of Sciences, Dr Bohr Gasse 3 5, A 1030 Vienna, Austria
    Nat Neurosci 8:156-63. 2005
    ..We show here, however, that ubiquitination of Comm is not required for its function in vitro or in vivo, and that Nedd4 is unlikely to function in axon guidance at the midline...
  101. ncbi Exploring embryonic germ line development in the water flea, Daphnia magna, by zinc-finger-containing VASA as a marker
    Kazunori Sagawa
    School of Life Science, Tokyo University of Pharmacy and Life Science, Hachioji, Japan
    Gene Expr Patterns 5:669-78. 2005
    ..RNA analyses suggested that maternally supplied vasa mRNA was responsible for early VASA expression, while zygotic expression started during blastodermal stage of development...

Research Grants69

  1. ANALYSIS OF PAIR OF RULE GENES IN SEQUENTIAL SEGMENTATIO
    DAVID WEISBLAT; Fiscal Year: 2003
    ..abstract_text> ..
  2. Spiralian developmental system for the study of multipotency
    Cesar Arenas Mena; Fiscal Year: 2008
    ..The ultimate goal is to manipulate these genes and reprogram in vivo differentiated cells into multipotent stem cells. [unreadable] [unreadable] [unreadable]..
  3. Redox-sensitive developmental pathways and gene regulatory networks
    JAMES COFFMAN; Fiscal Year: 2009
    ..Finally, the Comparative Toxicogenomics Database (CTD) at MDIBL will be used to determine the relevance of the pathways discovered in sea urchins to human health, and to generate hypotheses that might explain specific human diseases. ..
  4. Control of Cell Proliferation by Runx Proteins
    JAMES COFFMAN; Fiscal Year: 2009
    ..purpuratus genome sequence, which will greatly facilitate both the cis-regulatory analysis of genes and the identification of purified proteins by mass spectrometry. ..
  5. Bistability and Biological Oscillations
    JAMES FERRELL; Fiscal Year: 2009
    ..abstract_text> ..
  6. EARLY EVENTS IN NEURAL AND HOMEOGENETIC INDUCTION
    Claudio Stern; Fiscal Year: 2005
    ..D. To explore the possible roles of 6 newly-identified candidate secreted factors in the early steps of neural induction and homeogenetic induction. ..
  7. REGULATION OF BETA-CATENIN NUCLEARIZATION
    CHARLES ETTENSOHN; Fiscal Year: 2006
    ..In addition, using dominant negative constructs and morpholinos, we will examine the developmental function of two regulators of GSK3 activity, disheveled and Akt/PKB. ..
  8. BIOLOGY OF BAMACAN IN TUMORIGENESIS
    Giancarlo Ghiselli; Fiscal Year: 2006
    ..3) The contribution of bamacan in vivo to colon tumorigenesis by generating transgenic mice over-expressing bamacan in the intestinal epithelium. ..
  9. Development of gonadotropin releasing hormone cells
    KATHLEEN WHITLOCK; Fiscal Year: 2009
    ....
  10. DEVELOPMENT OF THE OLFACTORY SYSTEM IN ZEBRAFISH
    KATHLEEN WHITLOCK; Fiscal Year: 2004
    ..abstract_text> ..
  11. Proteolytic Control of Early Events in Mitosis
    JAMES FERRELL; Fiscal Year: 2008
    ..abstract_text> ..
  12. MOLECULAR AND CELLULAR BASIS OF LENS DEVELOPMENT
    Jonathan J Henry; Fiscal Year: 2010
    ....
  13. Regulatory Genomics: BAC-GFP Library of Control Genes
    Eric Davidson; Fiscal Year: 2008
    ..Numerous letters from outside investigators attesting interest in this facility are included as an Appendix to this application. ..
  14. MYOFIBRILLOGENESIS IN LIVING SKELETAL MUSCLE CELLS
    Joseph Sanger; Fiscal Year: 2004
    ..These approaches should yield new insights into basic and pathologic processes in myofibril assembly in skeletal muscle cells. ..
  15. Interdisciplinary Training in Quantitative Cell Biology
    RONALD VALE; Fiscal Year: 2006
    ..In this application we request support for this exciting endeavor. ..
  16. Modulation of radiosensitivity in zebrafish
    William Davidson; Fiscal Year: 2008
    ..This work will be performed by using state-of-the-art methods of downregulating gene expression or function and has important translational ramifications. ..
  17. MECHANISM & FUNCTIONS OF ICH-3 IN APOPTOSIS/INFLAMMATION
    Junying Yuan; Fiscal Year: 2001
    ..These works will have direct implication in control of apoptosis in normal and inflammatory conditions. ..
  18. Molecular mechanism of a cellular necrotic cell death pathway
    Junying Yuan; Fiscal Year: 2010
    ..Understanding the mechanism by which cells regulate necroptotic cell death may provide unique opportunities for developing novel therapies of major human diseases with little or no known treatment. ..
  19. REGULATION OF CYTOKINESIS IN ANIMAL CELLS
    David Burgess; Fiscal Year: 2004
    ..abstract_text> ..
  20. Genetic Analysis of Usher Syndrome in Zebrafish
    JONATHAN MATSUI; Fiscal Year: 2005
    ..Both morphological and physiological studies (e.g. ERG and auditory brain stem responses) will be used. Positional cloning strategies will be used to identify the genes responsible for these mutations. ..
  21. ORGANOGENESIS OF THE PHARYNX IN C ELEGANS
    Susan E Mango; Fiscal Year: 2010
    ..These studies will enable us to test the hypothesis that the loss of developmental plasticity is controlled by sequence-specific transcription factors in combination with global transcriptional regulatory mechanisms. ..
  22. Specification of embryonic C. elegans mesoderm by a T-box factor
    Morris Maduro; Fiscal Year: 2008
    ..elegans may shed light on how development goes awry, a process which can lead to birth defects or cancer. [unreadable] [unreadable] [unreadable]..
  23. Understanding Head Development: A Segmental Groundplan of Vertebrates
    Thomas Schilling; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  24. Development of Epibranchial Placodes and Ganglia in Zebrafish
    Alex Nechiporuk; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  25. Mathematical Tools for Developmental Signaling Networks
    CLAIRE TOMLIN; Fiscal Year: 2008
    ..The long term goals are to use this research to eventually aid in understanding the corresponding signaling in humans. ..
  26. Development of Anti-necrosis Drug for Acute Brain Injury
    Junying Yuan; Fiscal Year: 2008
    ..This project will culminate with the filing of an IND application with the FDA in order to enter a Phase I human clinical trial. [unreadable] [unreadable] [unreadable]..
  27. Mechanisms of Polycomb Silencing
    Yi Zhang; Fiscal Year: 2010
    ..5. .Determine the transcriptional effect and understand the mechanism of H3K27 methylation and H2A ubiquitylation using a reconstituted chromatin transcription system. More description in proposal. ..
  28. Targeted Manipulation of the Zebrafish Genome through Homologous Recombination
    Brian Ciruna; Fiscal Year: 2010
    ....
  29. NONSYNDROMIC CRANIOSYNOSTOSIS: PHENOTYPE/GENOTYPE STUDY
    SIMEON A BOYADJIEV BOYD; Fiscal Year: 2010
    ..In addition, we will also identify genes associated with sagittal NSC. The resources that we have amassed and the collaborative and integrated approaches that we will utilize put us in a unique position to accomplish these aims. ..
  30. Xenbase: a Xenopus Model Organism Database
    PETER VIZE; Fiscal Year: 2009
    ..abstract_text> ..
  31. Retinoid Signaling in Neural Patterning in Zebrafish
    Thomas Schilling; Fiscal Year: 2009
    ....
  32. Spatial Control of Proteolysis in Dorsoventral Polarity
    ELLEN LEMOSY; Fiscal Year: 2009
    ..abstract_text> ..
  33. Dynamics of Proteins in the A-bands of Cardiac Muscle
    Joseph Sanger; Fiscal Year: 2009
    ..The advanced imaging methods coupled with molecular biological and biochemical techniques should yield new insights into basic and pathologic processes in myofibril assembly in living cardiomyocytes. ..
  34. HORMONE ACTION ON THE CNS
    JAMES TRUMAN; Fiscal Year: 2009
    ..Understanding these issues is fundamental to normal brain development and for using stem cells to repair brain disease. ..
  35. Zebrafish Heart Mutants:Physiological & Genetic Analyses
    Neil Chi; Fiscal Year: 2008
    ..These zebrafish mutants ultimately may serve as a novel gateway to gain new insight into the cellular and molecular mechanisms of human cardiac arrhythmias and heart failure. ..
  36. Lipid Homeostasis in C. elegans
    Susan Mango; Fiscal Year: 2007
    ..unreadable] These three approaches will elucidate the transcriptional network for fat metabolism within the C. elegans digestive tract [unreadable] [unreadable]..
  37. Generation of a domain interaction map of C. elegans early embryogenesis
    Mike Boxem; Fiscal Year: 2007
    ..The integrated interaction network generated will therefore be very valuable to both C. elegans researchers and others studying animal development. [unreadable] [unreadable] [unreadable]..
  38. Regulation of neuronal stem cell death
    Erhard Bieberich; Fiscal Year: 2006
    ..In conclusion, this study will identify mechanisms for regulation of apoptosis by ceramide that are critical for normal brain development and/or the etiology of subsequent, pathological disorders in adulthood. ..
  39. Zebrafish in Comparative Context: A Symposium
    Thomas Schilling; Fiscal Year: 2006
    ..unreadable] [unreadable]..
  40. BIOMECHANICS OF LOOPING IN THE EMBRYONIC HEART
    LARRY TABER; Fiscal Year: 2003
    ..Defining the biomechanical forces involved in looping would provide insight into this morphogenetic process and thereby help researchers searching for the link between gene expression and looping morphology. ..
  41. Developmental Compartments of the Zebrafish Neurocranium
    Johann Eberhart; Fiscal Year: 2005
    ..Last, a forward genetic screen will be used to discover new mutations that affect the development of neurocranial cartilages. ..
  42. Role of caderhins in the developing zebrafish CV system
    Brian Bagatto; Fiscal Year: 2005
    ..These strengths together will produce a truly original and significant study. ..
  43. SONIC HEDGEHOG'S ROLE IN PATTERNING THE LIMB
    Clifford Tabin; Fiscal Year: 2004
    ..In these experiments the Ptc and Gli genes, which are too large for the chick viral vector system, we will be misexpressed in limbs of transgenic mice. ..
  44. Mechanism of Gene Silencing by DNA Methylation
    Yi Zhang; Fiscal Year: 2005
    ..The proposed studies will provide a thorough characterization of the MeCP1 complex. They will also reveal the potential targets and repression mechanism of the MeCP1 complex. ..
  45. ASSAY FOR DETERMINING DRUG TOXICITY
    Chuenlei Parng; Fiscal Year: 2004
    ..abstract_text> ..
  46. CCM proteins and vascular MAPK signal transduction
    Michael Malone; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  47. Cloning and expression of sodium channel beta subunits
    Amanda Hobson; Fiscal Year: 2006
    ..Understanding nodal formation can contribute to clarifying the triggers for demyelinating disease as well as potential therapies. [unreadable] [unreadable]..
  48. TRAINING PROGRAM IN DEVELOPMENTAL BIOLOGY
    Raymond Keller; Fiscal Year: 2003
    ..Support will be for the fist 3 years of graduate student or post- doctoral study, with the condition of good progress in training, or until individual awards are obtained. ..
  49. Regulation of Coronary Vessel Assembly and Growth
    Robert Tomanek; Fiscal Year: 2007
    ..Since cardiac development depends on timely and adequate vascularization, an understanding of coronary vasculogenesis and angiogenesis will provide an important foundation for our understanding of cardiac defects. ..
  50. Genome wide analysis and modeling of signal integration in the Drosophila ovary
    Stanislav Shvartsman; Fiscal Year: 2009
    ..Given the highly conserved nature of these signaling pathways, our results will provide quantitative insights into the mechanisms of signaling crosstalk in developing and adult tissues. ..
  51. RNA PROCESSING AND RIBONUCLEOPROTEIN
    Thoru Pederson; Fiscal Year: 2001
    ..The same studies should provide information on sequences required for nuclear export. These two inter-related projects test the hypothesis that the nucleolus is a more pluri-functional organelle than previously thought. ..
  52. Body Plan Formation in Early Mouse Embryo
    Yusuke Marikawa; Fiscal Year: 2004
    ....
  53. ANGIOGENIC THERAPY FOR INFARCTED AND ISCHEMIC HEART
    Robert Tomanek; Fiscal Year: 2003
    ..Such interventions have a number of advantages over more invasive interventions, e.g., the delivery of oxogenous growth factors or gene therapy. ..
  54. AVIAN VASCULAR FUNCTION
    Hiroko Nishimura; Fiscal Year: 2007
    ..The proposed projects will be supported by five distinguished consultants. [unreadable] [unreadable]..
  55. Control of Spemann's organizer
    Michael Sheets; Fiscal Year: 2007
    ..We will address our view by examining the role(s) of FGF signaling pathway(s) within the organizer and the transcription of chordin as they relate to the ultimate inducing functions of Spemann's organizer. [unreadable] [unreadable]..
  56. STEROID RECEPTORS AND CNS DEVELOPMENT
    JAMES TRUMAN; Fiscal Year: 2007
    ..5) Use EcR dominant-negative mutations to selectively suppress cytodynamic modules to establish the role of each on growth dynamics and on the final form of the arbor. ..
  57. Predoctoral Fellowship for Minority Students
    Lisa Chang; Fiscal Year: 2006
    ..Some of these include microarray screening, DNA-binding assays, microinjection, microsurgery, in-situ hybridization, antisense and dominant negative inhibition of gene function, and transgenics. ..
  58. REGULATION OF CARDIAC MYOGENESIS
    PARKER ANTIN; Fiscal Year: 2002
    ..This information will increase our understanding of both normal and abnormal heart development and may suggest ways to alter heart development or replication of cardiac myocytes. ..
  59. Hemichordartes and the Origin of Chordates
    John Gerhart; Fiscal Year: 2009
    ..siRNA interventions will be used to evaluate the role of the encoded gene products in development. ..
  60. Analysis of endocytosis in Caenorhabditis elegans
    Hanna Fares; Fiscal Year: 2006
    ..New genes identified in worms will also be studied in humans through collaborations with researchers who study this process. ..
  61. Cell Cycle Regulation by Checkpoints in Drosophila
    Tin Tin Su; Fiscal Year: 2006
    ..Aim 4) We will screen through existing mutagensensitive Drosophila mutants for their ability to regulate mitosis, S phase and cell death after irradiation, in order to uncover novel genes needed for proper response to DNA damage. ..
  62. An in vivo Screen for Radiation Sensitizers
    Tin Tin Su; Fiscal Year: 2008
    ..Successful completion of this project would establish a novel, rapid and effective primary screen in vivo for radiosensitizers with potential therapeutic value. [unreadable] [unreadable] [unreadable]..
  63. Cell Growth and Radiation Survival in Drosophila
    Tin Tin Su; Fiscal Year: 2007
    ..Understanding radiation survival in a multicellular context could help identify potential therapies for when we do not want cells to survive irradiation, such as in the treatment of cancer. ..
  64. GEISHA, a Chicken Embryo Gene Expression Resource
    PARKER ANTIN; Fiscal Year: 2009
    ..This centralized repository for in situ hybridization gene expression information will provide a valuable and comprehensive resource for the biomedical research community. ..
  65. STUDY OF INSULATOR FUNCTION IN DROSOPHILA
    HAINI CAI; Fiscal Year: 2005
    ..abstract_text> ..
  66. Functional Genomics in a Simple Model Metazoan
    MARK MARTINDALE; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  67. Site-specific transgenesis in zebrafish
    JAMES LISTER; Fiscal Year: 2008
    ..These tools will advance the usefulness of this model organism as a model for human development and disease. [unreadable] [unreadable] [unreadable]..