Genomes and Genes
Summary: The embryo of species other than mammals. For the chicken, use the specific CHICK EMBRYO.
Publications295 found, 100 shown here
- p53 activation by knockdown technologiesMara E Robu
University of Minnesota, Minneapolis, Minnesota, United States of America
PLoS Genet 3:e78. 2007..p53 inhibition could potentially be applicable to other systems to suppress off-target effects caused by other knockdown technologies...
- Controlling morpholino experiments: don't stop making antisenseJudith S Eisen
Institute of Neuroscience, 1254 University of Oregon, Eugene, OR 97403 1254, USA
Development 135:1735-43. 2008..We also discuss how the use of morpholinos can lead to misleading results, including off-target effects, and we suggest controls that will allow researchers to interpret morpholino experiments correctly...
- High-resolution in situ hybridization to whole-mount zebrafish embryosChristine Thisse
Department of Cell Biology, University of Virginia Health Sciences Center, PO Box 800732, 1300 Jefferson Park Avenue, Charlottesville, Virginia 22908, USA
Nat Protoc 3:59-69. 2008..The whole procedure takes only 3 days and, because ISH conditions are the same for each probe tested, allows high throughput analysis of zebrafish gene expression during embryogenesis...
- MicroRNA expression in zebrafish embryonic developmentErno Wienholds
Hubrecht Laboratory, Centre for Biomedical Genetics, 3584 CT Utrecht, The Netherlands
Science 309:310-1. 2005....
- Silberblick/Wnt11 mediates convergent extension movements during zebrafish gastrulationC P Heisenberg
Department of Anatomy and Developmental Biology, University College, London, UK
Nature 405:76-81. 2000..Our results provide genetic and experimental evidence that Wnt activity in lateral tissues has a crucial role in driving the convergent extension movements underlying vertebrate gastrulation...
- Control of chemokine-guided cell migration by ligand sequestrationBijan Boldajipour
Center for Molecular Biology of Inflammation, Von Esmarch Strasse 56, D 48149 Munster, Germany
Cell 132:463-73. 2008....
- Live imaging of wound inflammation in Drosophila embryos reveals key roles for small GTPases during in vivo cell migrationBrian Stramer
Department of Physiology, School of Medical Sciences, University of Bristol, Bristol, BS8 1TD, UK
J Cell Biol 168:567-73. 2005..Cdc42 is necessary for maintaining cellular polarity and yet, despite in vitro evidence, is dispensable for sensing and crawling toward wound cues...
- Cellular and molecular analyses of vascular tube and lumen formation in zebrafishSuk Won Jin
Department of Biochemistry and Biophysics and Cardiovascular Research Institute, Program in Developmental Biology, University of California San Francisco, 1550 Fourth Street, San Francisco, CA 94143, USA
Development 132:5199-209. 2005..These studies provide the tools and a cellular framework for the investigation of mutations affecting vasculogenesis in zebrafish...
- Loss of myotubularin function results in T-tubule disorganization in zebrafish and human myotubular myopathyJames J Dowling
Department of Pediatrics, University of Michigan Medical Center, Ann Arbor, Michigan, USA
PLoS Genet 5:e1000372. 2009..Based on our findings, we speculate that congenital myopathies, usually considered entities with similar clinical features but very disparate pathomechanisms, may at their root be disorders of calcium homeostasis...
- Fgf8 morphogen gradient forms by a source-sink mechanism with freely diffusing moleculesShuizi Rachel Yu
Developmental Genetics, Biotechnology Center, TUD, Tatzberg 47 49, 01307 Dresden, Germany
Nature 461:533-6. 2009..Our results demonstrate that a freely diffusing morphogen can set up concentration gradients in a complex multicellular tissue by a simple source-sink mechanism...
- Drosophila melanogaster embryonic haemocytes: masters of multitaskingWill Wood
Department of Biology and Biochemistry, University of Bath, BA2 7AY, UK
Nat Rev Mol Cell Biol 8:542-51. 2007..Instead, throughout development, embryonic haemocytes are occupied with other tasks: they undergo complex migrations and carry out several non-immune functions that are crucial for successful embryogenesis...
- Vertebrate Smoothened functions at the primary ciliumKevin C Corbit
Developmental and Stem Cell Biology Program, University of California, San Francisco, California 94143, USA
Nature 437:1018-21. 2005..Thus, Hh-dependent translocation to cilia is essential for Smo activity, suggesting that Smo acts at the primary cilium...
- RNA polymerase stalling at developmental control genes in the Drosophila melanogaster embryoJulia Zeitlinger
Whitehead Institute for Biomedical Research, Nine Cambridge Center, Cambridge, Massachusetts 02142, USA
Nat Genet 39:1512-6. 2007..We propose that Pol II stalling facilitates rapid temporal and spatial changes in gene activity during development...
- Zebrafish as a model organism for the identification and characterization of drugs and genes affecting p53 signalingUlrike Langheinrich
Exelixis Deutschland GmbH, Spemannstrasse 35, D 72076 Tubingen, Germany
Curr Biol 12:2023-8. 2002..We conclude that zebrafish represents a promising model organism for future compound-based and genetic screens and believe that it will help to identify and characterize new anticancer drugs and new targets for cancer treatment...
- Transgenesis and gene trap methods in zebrafish by using the Tol2 transposable elementKoichi Kawakami
Division of Molecular and Developmental Biology, National Institute of Genetics, Mishima, Shizuoka 411 8540, Japan
Methods Cell Biol 77:201-22. 2004
- Zebrafish MiR-430 promotes deadenylation and clearance of maternal mRNAsAntonio J Giraldez
Developmental Genetics Program, Skirball Institute of Biomolecular Medicine, and Department of Cell Biology, New York University School of Medicine, New York, NY 10016, USA
Science 312:75-9. 2006..We also show that miR-430 accelerates the deadenylation of target mRNAs. These results suggest that miR-430 facilitates the deadenylation and clearance of maternal mRNAs during early embryogenesis...
- CHD1 motor protein is required for deposition of histone variant H3.3 into chromatin in vivoAlexander Y Konev
Department of Cell Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
Science 317:1087-90. 2007..Furthermore, our results point to the general requirement of adenosine triphosphate (ATP)-utilizing motor proteins for histone deposition in vivo...
- sonic hedgehog and vascular endothelial growth factor act upstream of the Notch pathway during arterial endothelial differentiationNathan D Lawson
Laboratory of Molecular Genetics, NICHD, NIH, Bethesda, MD 20892, USA
Dev Cell 3:127-36. 2002..These studies reveal a complex signaling cascade responsible for establishing arterial cell fate and suggest differential effects of Vegf on developing endothelial cells...
- Zebrafish pea3 and erm are general targets of FGF8 signalingH Roehl
Max Planck Institut fur Entwicklungsbiologie, Spemannstrasse 35 III, D 72076, Tubingen, Germany
Curr Biol 11:503-7. 2001..To test whether this differential expression is established by FGF8, we have induced FGF8 ectopically and show that it is sufficient to recapitulate the endogenous nested expression pattern of pea3 and erm...
- Multiple forces contribute to cell sheet morphogenesis for dorsal closure in DrosophilaD P Kiehart
Department of Cell Biology, Cell and Molecular Biology Program, University Program in Genetics and the Duke Comprehensive Cancer Center, Duke University Medical Center, Durham, North Carolina 27710 3709, USA
J Cell Biol 149:471-90. 2000..Moreover, our observations demonstrate that wound healing in this insect epidermal system parallel wound healing in vertebrate tissues in situ and vertebrate cells in culture (for review see Kiehart, D.P. 1999. Curr. Biol. 9:R602-R605)...
- Mechanisms of asymmetric cell division: flies and worms pave the wayPIERRE GONCZY
Swiss Institute for Experimental Cancer Research ISREC, Swiss Federal Institute of Technology EPFL, School of Life Sciences, Lausanne, Switzerland
Nat Rev Mol Cell Biol 9:355-66. 2008..Lessons learned from these two model organisms also apply to cells that divide asymmetrically in other metazoans, such as self-renewing stem cells in mammals...
- Directional migration of neural crest cells in vivo is regulated by Syndecan-4/Rac1 and non-canonical Wnt signaling/RhoAHelen K Matthews
Department of Anatomy and Development Biology, University College London, Gower Street, London WC1E 6BT, UK
Development 135:1771-80. 2008..In addition, we show that RhoA inhibits Rac in NC cells. We present a model in which Syn4 and PCP control directional NC migration by, at least in part, regulating membrane protrusions through the regulation of small GTPase activities...
- Sepsid even-skipped enhancers are functionally conserved in Drosophila despite lack of sequence conservationEmily E Hare
Department of Molecular and Cell Biology, University of California Berkeley, Berkeley, California, United States of America
PLoS Genet 4:e1000106. 2008..Together, these observations suggest that the local arrangement of binding sites relative to each other is more important than their overall arrangement into larger units of cis-regulatory function...
- Gastrulation in Drosophila: the logic and the cellular mechanismsM Leptin
Institut fur Genetik, Universitat zu Koln, Weyertal 121, D 50931 Köln, Germany
EMBO J 18:3187-92. 1999....
- Pcl-PRC2 is needed to generate high levels of H3-K27 trimethylation at Polycomb target genesMaxim Nekrasov
Gene Expression Programme, EMBL, Heidelberg, Germany
EMBO J 26:4078-88. 2007..Pcl-PRC2 is thus the HMTase that generates the high levels of H3-K27 trimethylation in Polycomb target genes that are needed to maintain a Polycomb-repressed chromatin state...
- Fgf10 regulates hepatopancreatic ductal system patterning and differentiationP Duc Si Dong
Department of Biochemistry and Biophysics, Programs in Developmental Biology, Genetics and Human Genetics, and the Diabetes Center, University of California, San Francisco, 1550 Fourth Street, San Francisco, California 94158, USA
Nat Genet 39:397-402. 2007..These data shed light onto how the multipotent cells of the foregut endoderm, and subsequently those of the hepatopancreatic duct, are directed toward different organ fates...
- Multicellular rosette formation links planar cell polarity to tissue morphogenesisJ Todd Blankenship
Developmental Biology Program, Sloan Kettering Institute, New York, New York 10021, USA
Dev Cell 11:459-70. 2006..We propose that the generation of higher-order rosette structures links local cell interactions to global tissue reorganization during morphogenesis...
- The chromokinesin, KLP3A, dives mitotic spindle pole separation during prometaphase and anaphase and facilitates chromatid motilityMijung Kwon
University of California Davis, Davis, California 95616, USA
Mol Biol Cell 15:219-33. 2004..We propose that KLP3A acts on MTs associated with chromosome arms and the central spindle to organize ipMT bundles, to drive spindle pole separation and to facilitate chromatid motility...
- Pax6 induces ectopic eyes in a vertebrateR L Chow
Developmental Genetics Program, Skirball Institute of Biomolecular Medicine, New York University Medical Center, New York, NY 10016, USA
Development 126:4213-22. 1999..These data show that in vertebrates, as in the invertebrate Drosophila melanogaster, Pax6 is both necessary and sufficient to trigger the cascade of events required for eye formation...
- Efficient transposition of the Tol2 transposable element from a single-copy donor in zebrafishAkihiro Urasaki
Division of Molecular and Developmental Biology, National Institute of Genetics, and Department of Genetics, Graduate University for Advanced Studies SOKENDAI, Mishima, Shizuoka 411 8540, Japan
Proc Natl Acad Sci U S A 105:19827-32. 2008..Our present study demonstrates that the in vivo Tol2 transposition system is useful for creating genome-wide insertions from a single-copy donor and should facilitate functional genomics and transposon biology in vertebrates...
- Zebrafish Rho kinase 2 acts downstream of Wnt11 to mediate cell polarity and effective convergence and extension movementsFlorence Marlow
Department of Biological Sciences, Vanderbilt University, VU Station B 351634, Nashville, TN 37235 1634, USA
Curr Biol 12:876-84. 2002..Moreover, how defective mediolateral cell polarity impacts CE is not understood...
- A role for FGF-8 in the dorsoventral patterning of the zebrafish gastrulaM Fürthauer
Institut de Genetique et de Biologie Moleculaire et Cellulaire, CNRS, INSERM, ULP, Illkirch, France
Development 124:4253-64. 1997..We conclude that FGF-8 is involved in defining dorsoventral identity and is an important organizing factor responsible for specification of mesodermal and ectodermal dorsolateral territories of the zebrafish gastrula...
- Presynaptic development at the Drosophila neuromuscular junction: assembly and localization of presynaptic active zonesA Prokop
Department of Zoology, University of Cambridge, United Kingdom
Neuron 17:617-26. 1996..Thus, the process of synapse formation can be genetically separated from the process of target recognition, revealing that localization of presynaptic active zones requires mef2-dependent muscle differentiation...
- Characterization of a notochord-specific enhancer from the Brachyury promoter region of the ascidian, Ciona intestinalisJ C Corbo
Department of Biology, University of California, San Diego, La Jolla 92093 0347, USA
Development 124:589-602. 1997..We discuss the implications of this analysis with regard to the evolutionary conservation of integrative enhancers, and the subdivision of the axial and paraxial mesoderm in vertebrates...
- FoxH1 negatively modulates flk1 gene expression and vascular formation in zebrafishJayoung Choi
Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA 90095, USA
Dev Biol 304:735-44. 2007..Taken together, our data provide the first evidence that flk1 is a direct target of FoxH1 and that FoxH1 is involved in vessel formation in zebrafish...
- A role for Piwi and piRNAs in germ cell maintenance and transposon silencing in ZebrafishSaskia Houwing
Hubrecht Laboratory, Uppsalalaan 8, Utrecht, Netherlands
Cell 129:69-82. 2007..Furthermore, we show that piRNAs are Dicer independent and that their 3' end likely carries a 2'O-Methyl modification...
- Dishevelled controls apical docking and planar polarization of basal bodies in ciliated epithelial cellsTae Joo Park
Department of Molecular Cell and Developmental Biology and Institute for Cellular and Molecular Biology, University of Texas, Austin, Texas 78712, USA
Nat Genet 40:871-9. 2008..These results demonstrate previously undescribed functions for PCP signaling components and suggest that a common signaling apparatus governs both apical docking and planar polarization of basal bodies...
- p53-dependent neuronal cell death in a DJ-1-deficient zebrafish model of Parkinson's diseaseSandrine Bretaud
Centre for Developmental and Biomedical Genetics, University of Sheffield, Sheffield, UK
J Neurochem 100:1626-35. 2007..Our study demonstrates the utility of zebrafish as a new animal model to study PD gene defects and suggests that modulation of downstream mechanisms, such as p53 inhibition, may be of therapeutic benefit...
- Quantitative transcript imaging in normal and heat-shocked Drosophila embryos by using high-density oligonucleotide arraysR Leemans
Institute of Zoology, Biocenter Pharmacenter, University of Basel, CH 4056 Basel, Switzerland
Proc Natl Acad Sci U S A 97:12138-43. 2000..These results demonstrate that high-density oligonucleotide arrays are sensitive, efficient, and quantitative instruments for the analysis of large scale gene expression in Drosophila embryos...
- Dynamic analysis of filopodial interactions during the zippering phase of Drosophila dorsal closureThomas H Millard
Department of Biochemistry, School of Medical Sciences, University of Bristol, University Walk, Bristol BS8 1DA, UK
Development 135:621-6. 2008..Finally, we characterise the behaviour of a patched-expressing cell that we observe within the engrailed region of segments A1-A5, and provide evidence that this cell contributes to cell matching...
- SegmentationDiethard Tautz
Institut für Genetik der Universität zu Köln, Weyertal 121, 50931, Germany
Dev Cell 7:301-12. 2004..An old concept of comparative anatomy, the enterocoele theory, is compatible with a single origin of segmentation mechanisms and could therefore provide a conceptual framework for assessing these molecular similarities...
- Cranio-lenticulo-sutural dysplasia is caused by a SEC23A mutation leading to abnormal endoplasmic-reticulum-to-Golgi traffickingSimeon A Boyadjiev
McKusick Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
Nat Genet 38:1192-7. 2006..Our observations suggest that disrupted endoplasmic reticulum export of the secretory proteins required for normal morphogenesis accounts for CLSD...
- Silencing of Smed-betacatenin1 generates radial-like hypercephalized planariansMarta Iglesias
Departament de Genetica, Universitat de Barcelona, 08028 Barcelona, Spain
Development 135:1215-21. 2008..mediterranea beta-catenins could be functionally specialized and that only Smed-betacatenin1 is involved in Wnt signalling...
- Formation of the bicoid morphogen gradient: an mRNA gradient dictates the protein gradientAlexander Spirov
Center for Developmental Genetics, Stony Brook University, Stony Brook, NY 11794 5140, USA
Development 136:605-14. 2009....
- The zebrafish spi1 promoter drives myeloid-specific expression in stable transgenic fishAlister C Ward
Centre for Cellular and Molecular Biology, School of Biological and Chemical Sciences, Deakin University, Burwood, Victoria, Australia
Blood 102:3238-40. 2003..These TG(spi1:EGFP)pA301 transgenic zebrafish represent a valuable tool for further studies of myeloid development and its perturbation...
- Primary culture of Caenorhabditis elegans developing embryo cells for electrophysiological, cell biological and molecular studiesKevin Strange
Department of Anesthesiology, Vanderbilt University Medical Center, Nashville, Tennessee 37232, USA
Nat Protoc 2:1003-12. 2007..Cultured embryo cells are well suited for physiological analysis as well as molecular and cell biological studies. The embryo cell isolation protocol can be completed in 5-6 h...
- Neurodevelopmental defects in zebrafish (Danio rerio) at environmentally relevant dioxin (TCDD) concentrationsAdrian Hill
Laboratory for Environmental Gene Regulation, School of Biological Sciences, Biosciences Building, University of Liverpool, Crown Street, Liverpool Merseyside L69 7ZB, United Kingdom
Toxicol Sci 76:392-9. 2003..This disruption of neuronal development provides the basis for understanding the neurotoxic effects of these compounds...
- Comm function in commissural axon guidance: cell-autonomous sorting of Robo in vivoKrystyna Keleman
Institute of Molecular Biotechnology of the Austrian Academy of Sciences, Dr Bohr Gasse 3 5, A 1030 Vienna, Austria
Nat Neurosci 8:156-63. 2005..We show here, however, that ubiquitination of Comm is not required for its function in vitro or in vivo, and that Nedd4 is unlikely to function in axon guidance at the midline...
- Whole-somite rotation generates muscle progenitor cell compartments in the developing zebrafish embryoGeorgina E Hollway
The Victor Chang Cardiac Research Institute, 384 Victoria Street, Darlinghurst, New South Wales 2010, Australia
Dev Cell 12:207-19. 2007..We also show that formation of this compartment occurs via whole-somite rotation, a process that requires the action of the Sdf family of secreted cytokines...
- Cortical flows powered by asymmetrical contraction transport PAR proteins to establish and maintain anterior-posterior polarity in the early C. elegans embryoEdwin Munro
Center for Cell Dynamics, Friday Harbor, WA 98250, USA
Dev Cell 7:413-24. 2004..Similar actomyosin flows accompany the establishment of PAR asymmetries that form after the one-cell stage, suggesting that actomyosin-mediated cortical flows have a general role in PAR asymmetry...
- Scl is required for dorsal aorta as well as blood formation in zebrafish embryosLucy J Patterson
Weatherall Institute of Molecular Medicine, Oxford University, John Radcliffe Hospital, Headington, Oxford, OX3 9DS, United Kingdom
Blood 105:3502-11. 2005..We conclude that scl is especially critical for the development of arteries where adult hematopoietic stem cells emerge, implicating scl in the formation of hemogenic endothelium...
- Exploring embryonic germ line development in the water flea, Daphnia magna, by zinc-finger-containing VASA as a markerKazunori Sagawa
School of Life Science, Tokyo University of Pharmacy and Life Science, Hachioji, Japan
Gene Expr Patterns 5:669-78. 2005..RNA analyses suggested that maternally supplied vasa mRNA was responsible for early VASA expression, while zygotic expression started during blastodermal stage of development...
- Multiplex detection of RNA expression in Drosophila embryosDave Kosman
Section of Cell and Developmental Biology, University of California San Diego, La Jolla, CA 92093, USA
Science 305:846. 2004....
- The role of the segmentation gene hairy in TriboliumManuel Aranda
Department of Genetics, University of Cologne, Zülpicherstrasse 47, 50674, Koln, Germany
Dev Genes Evol 218:465-77. 2008..Our results imply that there is considerable divergence in hairy function between Tribolium and Drosophila...
- How the Dorsal gradient works: insights from postgenome technologiesJoung Woo Hong
Division of Genetics, Genomics, and Development, Department of Molecular and Cell Biology, Center for Integrative Genomics, University of California, Berkeley, CA 94720, USA
Proc Natl Acad Sci U S A 105:20072-6. 2008..The identification of 60-70 Dorsal target genes, along with the characterization of approximately 35 associated regulatory DNAs, suggests that there are at least six different regulatory codes driving diverse DV expression profiles...
- Postsynaptic filopodia in muscle cells interact with innervating motoneuron axonsS Ritzenthaler
Department of Cell and Structural Biology, University of Illinois, B605 CLS Laboratory, 601 South Goodwin Ave, Urbana, Illinois 61801, USA
Nat Neurosci 3:1012-7. 2000..Thus, postsynaptic filopodia are capable of intimate interaction with innervating presynaptic axons. We propose that, by contributing to direct long-distance cellular communication, they are dynamically involved in synaptic matchmaking...
- Cell cycle control of chorion gene amplificationB R Calvi
Howard Hughes Medical Institute Research Laboratories, Carnegie Institution of Washington, Baltimore, Maryland 21210, USA
Genes Dev 12:734-44. 1998..Our findings suggest that chorion amplification is a model for understanding metazoan replicons and the controls that restrict replication to once per cell cycle...
- The A- and B-type cyclins of Drosophila are accumulated and destroyed in temporally distinct events that define separable phases of the G2-M transitionW G Whitfield
Department of Biochemistry, The University, Dundee, Scotland
EMBO J 9:2563-72. 1990..These observations suggest that cyclin A has a role in the G2-M transition that is independent of spindle formation, and that entry into anaphase is a key requirement for the degradation of cyclin B...
- Characterization of zebrafish mutants with defects in embryonic hematopoiesisD G Ransom
Max Planck Institut für Entwickslungbiologie, Abteilung Genetik, Tubingen, Germany
Development 123:311-9. 1996..The analysis of these mutants provides a powerful approach towards defining the molecular mechanisms involved in vertebrate hematopoietic development...
- Activation of transcription in Drosophila embryos is a gradual process mediated by the nucleocytoplasmic ratioD K Pritchard
Department of Genetics, University of Washington, Seattle, Washington, USA
Genes Dev 10:1131-42. 1996....
- Whole-genome ChIP-chip analysis of Dorsal, Twist, and Snail suggests integration of diverse patterning processes in the Drosophila embryoJulia Zeitlinger
Whitehead Institute for Biomedical Research, Nine Cambridge Center, Cambridge, Massachusetts 02142, USA
Genes Dev 21:385-90. 2007..Thus, the ChIP-chip data uncover a much larger than expected regulatory network, which integrates diverse patterning processes during development...
- Gateway compatible vectors for analysis of gene function in the zebrafishJacques A Villefranc
Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
Dev Dyn 236:3077-87. 2007..Taken together, this work provides an important platform for the rapid functional analyses of open reading frames in zebrafish embryos...
- rst and its paralogue kirre act redundantly during embryonic muscle development in DrosophilaM Strünkelnberg
Institut fur Biologie III, Schänzlestr 1, Albert Ludwigs Universitat, D 79104 Freiburg im Breisgau, Germany
Development 128:4229-39. 2001..This defect can be rescued by one copy of either gene. Moreover, Rst, like Kirre is a myoblast attractant...
- XCtBP is a XTcf-3 co-repressor with roles throughout Xenopus developmentM Brannon
Department of Biochemistry, Howard Hughes Medical Institute, Center for Developmental Biology, University of Washington School of Medicine, Seattle, Washington 98195 7350, USA
Development 126:3159-70. 1999..Our data support a role for XCtBP as a co-repressor throughout Xenopus development and indicate that XCtBP/G4A will be a useful tool in determining how XCtBP functions in various developmental processes...
- Analysis of genetic mosaics in developing and adult Drosophila tissuesT Xu
Howard Hughes Medical Institute, University of California, Berkeley 94720
Development 117:1223-37. 1993....
- Croquemort, a novel Drosophila hemocyte/macrophage receptor that recognizes apoptotic cellsN C Franc
Division of Hematology, Children s Hospital, Boston, Massachusetts 02115, USA
Immunity 4:431-43. 1996..Our findings support the idea that the primordial function of macrophages may have been in tissue modeling and that their adapted role is in host defense...
- CD41+ cmyb+ precursors colonize the zebrafish pronephros by a novel migration route to initiate adult hematopoiesisJulien Y Bertrand
Division of Biological Sciences, University of California, San Diego, La Jolla, CA 92093 0380, USA
Development 135:1853-62. 2008..Together, these studies suggest that expression of CD41 and cmyb marks nascent HSCs in the zebrafish AGM, and provide the means to further dissect HSC generation and function in the early vertebrate embryo...
- TAF1 activates transcription by phosphorylation of serine 33 in histone H2BTobias Maile
Department of Biochemistry, University of California Riverside, Riverside, CA 95121, USA
Science 304:1010-4. 2004..These data reveal that H2B-S33 is a physiological substrate for the TAF1 CTK and that H2B-S33 phosphorylation is essential for transcriptional activation events that promote cell cycle progression and development...
- Chk1 suppresses a caspase-2 apoptotic response to DNA damage that bypasses p53, Bcl-2, and caspase-3Samuel Sidi
Department of Pediatric Oncology, Dana Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA
Cell 133:864-77. 2008..The evolutionarily conserved "Chk1-suppressed" pathway defines a novel apoptotic process, whose responsiveness to Chk1 inhibitors and insensitivity to p53 and BCL2 alterations have important implications for cancer therapy...
- Zebrafish embryos as models for embryotoxic and teratological effects of chemicalsLixin Yang
Institute of Toxicology and Genetics, Forschungszentrum Karlsruhe in the Helmholtz Association, Karlsruhe Institute of Technology, PO Box 3640, Karlsruhe 76021, Germany
Reprod Toxicol 28:245-53. 2009....
- The protooncogene c-myc is an essential regulator of neural crest formation in xenopusAmy Bellmeyer
Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, IL 60208, USA
Dev Cell 4:827-39. 2003..These effects are not dependent upon changes in cell proliferation or cell death. Instead, our findings reveal an important and unexpected role for c-Myc in the specification of cell fates in the early ectoderm...
- Regulation of neurogenesis by interkinetic nuclear migration through an apical-basal notch gradientFilippo Del Bene
Department of Physiology, Programs in Neuroscience, Genetics, and Developmental Biology, University of California San Francisco, San Francisco, CA 94158 2722, USA
Cell 134:1055-65. 2008..Our data indicate that the function of INM is to balance the exposure of progenitor nuclei to neurogenic versus proliferative signals...
- Regulation of cytokinesis by Rho GTPase fluxAnn L Miller
Department of Zoology, University of Wisconsin Madison, Madison, WI 53706, USA
Nat Cell Biol 11:71-7. 2009....
- Zinc transporter LIVI controls epithelial-mesenchymal transition in zebrafish gastrula organizerSusumu Yamashita
Laboratory of Developmental Immunology, Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka 565 0871, Japan
Nature 429:298-302. 2004..Furthermore, we demonstrate that LIV1 is essential for the nuclear localization of zinc-finger protein Snail, a master regulator of EMT. These results establish a molecular link between STAT3, LIV1 and Snail in EMT...
- Distinct mechanisms regulate hemocyte chemotaxis during development and wound healing in Drosophila melanogasterWill Wood
Instituto Gulbenkian de Ciencia, 2780 156 Oeiras, Portugal
J Cell Biol 173:405-16. 2006....
- Fgf8 drives myogenic progression of a novel lateral fast muscle fibre population in zebrafishJulie A Groves
MRC Centre for Developmental Neurobiology and Randall Division for Cell and Molecular Biophysics, New Hunt s House, King s College London, London SE1 1UL, UK
Development 132:4211-22. 2005..We conclude that Fgf8 drives terminal differentiation of a specific population of lateral muscle precursor cells within the early somite...
- The specification of dorsal cell fates in the vertebrate central nervous systemK J Lee
Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biophysics, Center for Neurobiology and Behavior, Columbia University, New York, New York 10032, USA
Annu Rev Neurosci 22:261-94. 1999..Additional signals involving members of the Wnt and fibroblast growth factor (FGF) families may also contribute to the proliferation and differentiation of dorsal neuronal cell types...
- Mechanisms of Wnt signaling in developmentA Wodarz
Institut fur Genetik, Universitat Dusseldorf, Germany
Annu Rev Cell Dev Biol 14:59-88. 1998..Here we review recent data that have started to unravel the mechanisms of Wnt signaling...
- RIC-8 is required for GPR-1/2-dependent Galpha function during asymmetric division of C. elegans embryosKatayoun Afshar
Swiss Institute for Experimental Cancer Research ISREC, 1066 Epalinges Lausanne, Switzerland
Cell 119:219-30. 2004..Our findings suggest a mechanism in which RIC-8 favors generation of Galpha free from Gbetagamma and enables GPR-1/2 to mediate asymmetric cell division...
- A quantitative spatiotemporal atlas of gene expression in the Drosophila blastodermCharless C Fowlkes
Berkeley Drosophila Transcription Network Project, University of California, Irvine, CA 92697, USA
Cell 133:364-74. 2008..We present a VirtualEmbryo containing data for 95 genes at six time cohorts. We show that known gene-regulatory interactions can be automatically recovered from this data set and predict hundreds of new interactions...
- Cooperation of polarized cell intercalations drives convergence and extension of presomitic mesoderm during zebrafish gastrulationChunyue Yin
Department of Biological Sciences, Vanderbilt University, Nashville, TN 37235, USA
J Cell Biol 180:221-32. 2008....
- Indirect effects of ploidy suggest X chromosome dose, not the X:A ratio, signals sex in DrosophilaJames W Erickson
Department of Biology, Texas A and M University, College Station, Texas, United States of America
PLoS Biol 5:e332. 2007..Our findings reiterate that correlations between X:A ratios and cell fates in other organisms need not implicate the value of the ratio as an active signal...
- Epigenetic memory of an active gene state depends on histone H3.3 incorporation into chromatin in the absence of transcriptionRay Kit Ng
Wellcome Trust Cancer Research UK Gurdon Institute, Tennis Court Road, Cambridge CB2 1QN, UK
Nat Cell Biol 10:102-9. 2008..Hence, we suggest that epigenetic memory helps to stabilize gene expression in normal development; it might also help to account for the inefficient reprogramming in some transplanted nuclei...
- Effects of RAS on the genesis of embryonal rhabdomyosarcomaDavid M Langenau
Stem Cell Program and Division of Hematology, Children s Hospital Boston and Dana Farber Cancer Institute, Boston, Massachusetts 02115, USA
Genes Dev 21:1382-95. 2007..When coupled with gene expression studies of this cell population, we propose that the zebrafish RMS cancer stem cell shares similar self-renewal programs as those found in activated satellite cells...
- Fluorescent in situ hybridization protocols in Drosophila embryos and tissuesEric Lecuyer
Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON, Canada
Methods Mol Biol 420:289-302. 2008..We also describe variations of the method for RNA-RNA and RNA-protein codetection...
- Unexpected novel relational links uncovered by extensive developmental profiling of nuclear receptor expressionStephanie Bertrand
Molecular Zoology, Institut de Génomique Fonctionelle de Lyon UMR 5242 du CNRS, INRA, IFR128 Biosciences Lyon Gerland, Universite de Lyon, UCB Ecole Normale Supérieure de Lyon, France
PLoS Genet 3:e188. 2007..Altogether, this expression database of NRs provides novel routes for leading investigation into the biological function of each individual NR as well as for the study of their combinatorial regulatory circuitry within the superfamily...
- Induction of the Xenopus organizer: expression and regulation of Xnot, a novel FGF and activin-regulated homeo box geneG von Dassow
Department of Biochemistry, University of Washington, Seattle 98195
Genes Dev 7:355-66. 1993..Our results provide experimental evidence supporting the proposed division of Spemann's organizer into independently regulated organizing centers...
- Shaping a morphogen gradient for positional precisionFeng He
Division of Biomedical Informatics, Cincinnati Children s Hospital Research Foundation, Cincinnati, Ohio, USA
Biophys J 99:697-707. 2010..Our results demonstrate that the positional information provided by the wt Bcd gradient profile is highly precise and necessary for patterning precision...
- An epidermal barrier wound repair pathway in Drosophila is mediated by grainy headKimberly A Mace
Section of Cell and Developmental Biology, Division of Biology, University of California, San Diego, La Jolla, CA 92093, USA
Science 308:381-5. 2005..The conservation of this Grh-dependent pathway suggests that the repair of insect cuticle and mammal skin is controlled by an ancient, shared control system for constructing and healing the animal body surface barrier...
- RISC assembly defects in the Drosophila RNAi mutant armitageYukihide Tomari
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
Cell 116:831-41. 2004..Our results suggest that armi is required for RISC maturation...
- Identification and real-time imaging of a myc-expressing neutrophil population involved in inflammation and mycobacterial granuloma formation in zebrafishAnnemarie H Meijer
Institute of Biology, Leiden University, Wassenaarseweg 64, 2333 AL Leiden, The Netherlands
Dev Comp Immunol 32:36-49. 2008..Using real-time confocal imaging we demonstrate that these cells migrate to sites of inflammation and are involved in innate immune responses towards infections, including Mycobacterium marinum-induced granuloma formation...
- Normal table of postembryonic zebrafish development: staging by externally visible anatomy of the living fishDavid M Parichy
Department of Biology, University of Washington, Seattle, Washington 91895, USA
Dev Dyn 238:2975-3015. 2009..Finally, we provide guidelines for reporting stages that provide information on both discrete and continuous changes in growth and development...
- Revisiting the role of the mother centriole in centriole biogenesisA Rodrigues-Martins
Instituto Gulbenkian de Ciencia, Cell Cycle Regulation Laboratory, Rua da Quinta Grande, 6, P 2780 156 Oeiras, Portugal
Science 316:1046-50. 2007..The mother centriole is not a bona fide template but a platform for a set of regulatory molecules that catalyzes and regulates daughter centriole assembly...
- Glycogen synthase kinase-3 and dorsoventral patterning in Xenopus embryosX He
Varmus Laboratory, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
Nature 374:617-22. 1995..These results indicate that GSK-3 is required for ventral differentiation, and suggest that dorsal differentiation may involve the suppression of GSK-3 activity by a wingless/wnt-related signal...
- The Drosophila patched gene encodes a putative membrane protein required for segmental patterningJ E Hooper
Howard Hughes Medical Institute, University of Colorado, Boulder 80309 0347
Cell 59:751-65. 1989..The pattern of expression does not directly predict the transformation seen in ptc mutant embryos, suggesting that ptc participates in cell interactions that establish pattern within the segment...
- Dnmt2 functions in the cytoplasm to promote liver, brain, and retina development in zebrafishKunal Rai
Howard Hughes Medical Institute, University of Utah, Salt Lake City, Utah 84112, USA
Genes Dev 21:261-6. 2007..Furthermore, zebrafish Dnmt2 methylates an RNA species of approximately 80 bases, consistent with tRNA methylation. Thus, Dnmt2 promotes zebrafish development, likely through cytoplasmic RNA methylation...
- Inhibition of Müller glial cell division blocks regeneration of the light-damaged zebrafish retinaRyan Thummel
Department of Biological Sciences and the Center for Zebrafish Research, University of Notre Dame, Notre Dame, Indiana 46556, USA
Dev Neurobiol 68:392-408. 2008....
- Dishevelled: a mobile scaffold catalyzing developmentCraig C Malbon
Department of Pharmacology, State University of New York at Stony Brook, Stony Brook, New York 11794, USA
Curr Top Dev Biol 72:153-66. 2006..Much excitement awaits the elucidation of the complete set of tools in the toolbox and of the dynamic regulation of Dishevelled proteins and their interacting proteins...
- Size control: cell proliferation does not equal growthT T Su
Department of Biochemistry and Biophysics, University of California San Francisco 94143 0448, USA
Curr Biol 8:R687-9. 1998..Both classic and recent experiments confirm this simple rationale: altering proliferation produces normally sized body structures with either especially small or exceptionally large cells...
- Origin and shaping of the laterality organ in zebrafishPablo Oteiza
Laboratory of Experimental Ontogeny, Program of Anatomy and Developmental Biology, Institute of Biomedical Sciences, University of Chile, Clasificador 7 Correo 7, Santiago, Chile
Development 135:2807-13. 2008..Our results provide novel insights into the morphogenetic transformations that shape the laterality organ in zebrafish and suggest a conserved progenitor role of the surface epithelium during laterality organ formation in vertebrates...
- Dynamic changes in the distribution of cytoplasmic myosin during Drosophila embryogenesisP E Young
Department of Cellular and Developmental Biology, Harvard University, Cambridge, MA 02138
Development 111:1-14. 1991....
Research Grants166 found, 100 shown here
- TGF-beta and axis formation in Helobdella, a lophotrochozoanDAVID WEISBLAT; Fiscal Year: 2007..They will also contribute to understanding the cellular processes known as epithelial-to-mesenchymal transitions (EMTs), which play a critical role both in normal development and in cancer metastasis. ..
- ANALYSIS OF PAIR OF RULE GENES IN SEQUENTIAL SEGMENTATIODAVID WEISBLAT; Fiscal Year: 2003..abstract_text> ..
- TGF-beta and axis formation in Helobdella, a lophotrochozoanDavid A Weisblat; Fiscal Year: 2010..They will also contribute to understanding the cellular processes known as epithelial-to-mesenchymal transitions (EMTs), which play a critical role both in normal development and in cancer metastasis. ..
- Spiralian developmental system for the study of multipotencyCesar Arenas Mena; Fiscal Year: 2007..The first objective is to identify the effector genes that set the peculiar transcriptional state of multipotency. The ultimate goal is to manipulate these genes and reprogram in vivo differentiated cells into multipotent stem cells. ..
- Cadherin6 and -10 Function in Retinal Ganglion and Amacrine Cell DevelopmentQin Liu; Fiscal Year: 2007....
- Control of Cell Proliferation by Runx ProteinsJAMES COFFMAN; Fiscal Year: 2009..purpuratus genome sequence, which will greatly facilitate both the cis-regulatory analysis of genes and the identification of purified proteins by mass spectrometry. ..
- Control of Cell Proliferation by Runx ProteinsJAMES COFFMAN; Fiscal Year: 2007..purpuratus genome sequence, which will greatly facilitate both the cis-regulatory analysis of genes and the identification of purified proteins by mass spectrometry. ..
- Regulation of Epidermal Junctions in C. elegansJEFFREY HARDIN; Fiscal Year: 2005..Moreover, this freely available, open-source suite will allow investigators and educators easy and inexpensive access to 4D analysis and acquisition capabilities using easily extensible, platform-independent Java technologies. ..
- Regulation of Epidermal Junctions in C. elegansJEFFREY HARDIN; Fiscal Year: 2007..Because these studies will be carried out in a living organism, we will gain fundamental new insights into how these key junctional complexes regulate a fundamental morphogenetic event. ..
- Redox-sensitive developmental pathways and gene regulatory networksJAMES COFFMAN; Fiscal Year: 2007..Finally, the Comparative Toxicogenomics Database (CTD) at MDIBL will be used to determine the relevance of the pathways discovered in sea urchins to human health, and to generate hypotheses that might explain specific human diseases. ..
- CONTROL OF EPITHELIAL SHEET MOVEMENT IN C ELEGANSJEFFREY HARDIN; Fiscal Year: 2003..As a result of these studies, we expect to gain a greater understanding of how specific lesions in identified molecules give rise to specific cellular defects during epithelial morphogenesis. ..
- Redox-sensitive developmental pathways and gene regulatory networksJAMES COFFMAN; Fiscal Year: 2009..Finally, the Comparative Toxicogenomics Database (CTD) at MDIBL will be used to determine the relevance of the pathways discovered in sea urchins to human health, and to generate hypotheses that might explain specific human diseases. ..
- Mechanisms of Junctional Actin Recruitment in C. elegansJEFFREY HARDIN; Fiscal Year: 2009..By studying how this protein works in living embryos, we will gain important information that can be used to understand and treat human disease. ..
- The Molecular Basis of Serpin Function and DisfunctionPatrick L Wintrode; Fiscal Year: 2010..Polymerization of alpha-1 antitrypsin is the underlying cause of these symptoms. Determining the structure of the polymerization prone intermediate is an important step in developing therapeutic agents. ..
- Mechanisms of Junctional Actin Recruitment in C. elegansJEFFREY D HARDIN; Fiscal Year: 2010..By studying how this protein works in living embryos, we will gain important information that can be used to understand and treat human disease. ..
- SIGNALING CASCADES AND LOOPS IN XENOPUS OOCYTESJAMES FERRELL; Fiscal Year: 2003..4. To determine whether regulated nuclear translocation contributes to the ultrasensitive response of p42 MAPK to upstream activators. ..
- Regulatory Genomics: BAC-GFP Library of Control GenesEric Davidson; Fiscal Year: 2007..Numerous letters from outside investigators attesting interest in this facility are included as an Appendix to this application. ..
- MOLECULAR AND CELLULAR BASIS OF LENS DEVELOPMENTJonathan J Henry; Fiscal Year: 2010....
- Bistability and the Mitotic TriggerJAMES FERRELL; Fiscal Year: 2009..An understanding of the process also promises to shed light on cancer and cancer chemotherapy. ..
- HOMOZYGOUS SEA URCHIN AS A POTENTIAL RESEARCH RESOURCEEric Davidson; Fiscal Year: 2003..purpuratus is a heritable trait, and carry out other investigations on genetic aspects of sex in S. purpuratus. ..
- MAP KINASE AND THE CELL CYCLEJAMES FERRELL; Fiscal Year: 2001..How does the MAPK cascade bring about Cdc2 activation? 4. How does activation of the MAPK cascade bring about G2 arrest in cycling extracts and embryos, and what is the significance of this arrest? ..
- An in vivo Screen for Radiation SensitizersTin Tin Su; Fiscal Year: 2007..Successful completion of this project would establish a novel, rapid and effective primary screen in vivo for radiosensitizers with potential therapeutic value. ..
- Predoctoral Fellowship for Minority StudentsLisa Chang; Fiscal Year: 2006..Some of these include microarray screening, DNA-binding assays, microinjection, microsurgery, in-situ hybridization, antisense and dominant negative inhibition of gene function, and transgenics. ..
- Regulation of Radiation Induced Cell Death in DrosophilaTin Tin Su; Fiscal Year: 2010..Because of excellent conservation of gene function between Drosophila and human, what we learn may allow us to maximize the efficacy of radiation therapy of human cancers. ..
- Regulation of Radiation Induced Cell Death in DrosophilaTin Tin Su; Fiscal Year: 2010..Because of excellent conservation of gene function between Drosophila and human, what we learn may allow us to maximize the efficacy of radiation therapy of human cancers. ..
- GEISHA, a Chicken Embryo Gene Expression ResourcePARKER ANTIN; Fiscal Year: 2009..This centralized repository for in situ hybridization gene expression information will provide a valuable and comprehensive resource for the biomedical research community. ..
- Cell Growth and Radiation Survival in DrosophilaTin Tin Su; Fiscal Year: 2007..Understanding radiation survival in a multicellular context could help identify potential therapies for when we do not want cells to survive irradiation, such as in the treatment of cancer. ..
- Hemichordartes and the Origin of ChordatesJohn Gerhart; Fiscal Year: 2009..siRNA interventions will be used to evaluate the role of the encoded gene products in development. ..
- Delineating the Role of FGF Signaling and Vertebrate Heart DevelopmentMICHAEL WAIKOK TSANG; Fiscal Year: 2010..Understanding how this molecule acts to increase heart tissue is an important step towards developing potential treatment for cardiac damage caused by heart disease. ..
- GEISHA, a Chicken Embryo Gene Expression ResourcePARKER ANTIN; Fiscal Year: 2007..This centralized repository for in situ hybridization gene expression information will provide a valuable and comprehensive resource for the biomedical research community. ..
- Delineating the Role of FGF Signaling and Vertebrate Heart DevelopmentMichael Tsang; Fiscal Year: 2009..Understanding how this molecule acts to increase heart tissue is an important step towards developing potential treatment for cardiac damage caused by heart disease. ..
- MicroRNA Gene Expression Mapping in Chicken EmbryosPARKER ANTIN; Fiscal Year: 2007..This new technology could fundamentally change the way in which in situ hybridization analyses are performed. ..
- Hemichordartes and the Origin of ChordatesJohn Gerhart; Fiscal Year: 2007..siRNA interventions will be used to evaluate the role of the encoded gene products in development. ..
- In Vivo Analysis of the Mechanisms of Neural Crest MigrationPaul M Kulesa; Fiscal Year: 2010....
- Cell Cycle Regulation by Checkpoints in DrosophilaTin Tin Su; Fiscal Year: 2006..Aim 4) We will screen through existing mutagensensitive Drosophila mutants for their ability to regulate mitosis, S phase and cell death after irradiation, in order to uncover novel genes needed for proper response to DNA damage. ..
- Analysis of endocytosis in Caenorhabditis elegansHanna Fares; Fiscal Year: 2006..New genes identified in worms will also be studied in humans through collaborations with researchers who study this process. ..
- Hemichordates and the Origin of ChordatesJohn Gerhart; Fiscal Year: 2004..Knowledge of hemichordates will help to explain why chordates have certain aspects of their body plan and development, and by difference, what additions and modifications have been made only within the chordate line. ..