hydrostatic pressure


Summary: The pressure due to the weight of fluid.

Top Publications

  1. Ishimori T, Takahashi K, Goto M, Nakagawa S, Kasai Y, Konagaya Y, et al. Synergistic effects of high hydrostatic pressure, mild heating, and amino acids on germination and inactivation of Clostridium sporogenes spores. Appl Environ Microbiol. 2012;78:8202-7 pubmed publisher
    The synergistic effects of high hydrostatic pressure (HHP), mild heating, and amino acids on the germination of Clostridium sporogenes spores were examined by determining the number of surviving spores that returned to vegetative growth ..
  2. Schauwecker J, VON Eisenhart Rothe R, Burgkart R, Tuebel J, Schmitt M, Mittelmeier W, et al. Revitalization of human bone after extracorporeal high hydrostatic pressure treatment. Anticancer Res. 2011;31:1235-9 pubmed
    ..Yet previous studies have shown that high hydrostatic pressure (HHP) allows for complete devitalization of tumor-afflicted bone segments, while the biomechanical and ..
  3. Demazeau G, Rivalain N. High hydrostatic pressure and biology: a brief history. Appl Microbiol Biotechnol. 2011;89:1305-14 pubmed publisher
    ..The introduction of high hydrostatic pressure (HHP) in Biology was an important scientific feature over the last hundred years...
  4. Lei Y, Rajabi S, Pedrigi R, Overby D, Read A, Ethier C. In vitro models for glaucoma research: effects of hydrostatic pressure. Invest Ophthalmol Vis Sci. 2011;52:6329-39 pubmed publisher
    ..Studies have reported the biological effects of hydrostatic pressure on ONH cells cultured on a rigid substrate...
  5. Gao J, Sun X, Moore L, White T, Brink P, Mathias R. Lens intracellular hydrostatic pressure is generated by the circulation of sodium and modulated by gap junction coupling. J Gen Physiol. 2011;137:507-20 pubmed publisher
    ..model suggested the channels could transport the secretion of aqueous humor, but flow would be driven by hydrostatic pressure rather than osmosis...
  6. Tamegai H, Ota Y, Haga M, Fujimori H, Kato C, Nogi Y, et al. Piezotolerance of the respiratory terminal oxidase activity of the piezophilic Shewanella violacea DSS12 as compared with non-piezophilic Shewanella species. Biosci Biotechnol Biochem. 2011;75:919-24 pubmed
    ..piezophile Shewanella violacea DSS12 is known to alter its respiratory components under the influence of hydrostatic pressure during growth, suggesting that it has a respiratory system that functions in adaptation to high pressure...
  7. Negishi J, Funamoto S, Kimura T, Nam K, Higami T, Kishida A. Effect of treatment temperature on collagen structures of the decellularized carotid artery using high hydrostatic pressure. J Artif Organs. 2011;14:223-31 pubmed publisher
    ..A decellularizing method based on high hydrostatic pressure (HHP) has been developed, and the preparation of many types of decellularized tissues has been investigated,..
  8. D Souza T, Karwe M, Schaffner D. Effect of high hydrostatic pressure and pressure cycling on a pathogenic Salmonella enterica serovar cocktail inoculated into creamy peanut butter. J Food Prot. 2012;75:169-73 pubmed publisher
    ..This study investigates the potential of high hydrostatic pressure processing, including pressure cycling, to inactivate Salmonella inoculated into creamy peanut butter...
  9. Huang C, Ogawa R. Effect of hydrostatic pressure on bone regeneration using human mesenchymal stem cells. Tissue Eng Part A. 2012;18:2106-13 pubmed publisher
    ..However, although hydrostatic pressure (HP) is a dominant and constant mechanical strain on bone cells in vivo, little is known about the effect ..

More Information


  1. Tabla R, Martinez B, Rebollo J, Gonzalez J, Ramirez M, Roa I, et al. Bacteriophage performance against Staphylococcus aureus in milk is improved by high hydrostatic pressure treatments. Int J Food Microbiol. 2012;156:209-13 pubmed publisher
    ..effect of bacteriophages, vB_SauS-phi-IPLA35 (phiIPLA35) and vB_SauS-phi-IPLA88 (phiIPLA88), and high hydrostatic pressure (HHP) on Staphylococcus aureus Sa9 was evaluated in pasteurized whole milk under a simulated cold chain ..
  2. Meyer E, Buckley C, Steward A, Kelly D. The effect of cyclic hydrostatic pressure on the functional development of cartilaginous tissues engineered using bone marrow derived mesenchymal stem cells. J Mech Behav Biomed Mater. 2011;4:1257-65 pubmed publisher
    ..The objective of this study was to determine if the long-term application of cyclic hydrostatic pressure could be used to improve the functional properties of cartilaginous tissues engineered using bone marrow ..
  3. Crapo P, Wang Y. Hydrostatic pressure independently increases elastin and collagen co-expression in small-diameter engineered arterial constructs. J Biomed Mater Res A. 2011;96:673-81 pubmed publisher
    ..that smooth muscle cell (SMC) proliferation, migration, and extracellular matrix production increase with hydrostatic pressure in vitro...
  4. Yu J, Zhong Y, Shen X, Cheng Y, Qi J, Wang J. In vitro effect of adenosine A2A receptor antagonist SCH 442416 on the expression of glutamine synthetase and glutamate aspartate transporter in rat retinal Müller cells at elevated hydrostatic pressure. Oncol Rep. 2012;27:748-52 pubmed publisher
    ..A2A receptor antagonist increased the expression of GLAST and GS of Müller cells and accelerated the clearance of extracellular glutamate. ..
  5. Thatje S, Robinson N. Specific dynamic action affects the hydrostatic pressure tolerance of the shallow-water spider crab Maja brachydactyla. Naturwissenschaften. 2011;98:299-313 pubmed publisher
    ..036; df = 2; p < 0.001). Elevated hydrostatic pressure is hypothesized to extend the duration and the total metabolic energy devoted to specific dynamic action...
  6. Oliphant A, Thatje S, Brown A, Morini M, Ravaux J, Shillito B. Pressure tolerance of the shallow-water caridean shrimp Palaemonetes varians across its thermal tolerance window. J Exp Biol. 2011;214:1109-17 pubmed publisher
    ..assessed the full physiological scope of a marine invertebrate species with respect to both temperature and hydrostatic pressure. In this study, adult specimens of the shallow-water shrimp species Palaemonetes varians were subjected to ..
  7. Steward A, Wagner D, Kelly D. The pericellular environment regulates cytoskeletal development and the differentiation of mesenchymal stem cells and determines their response to hydrostatic pressure. Eur Cell Mater. 2013;25:167-78 pubmed
    The objective of this study was to examine the interplay between matrix stiffness and hydrostatic pressure (HP) in regulating chondrogenesis of mesenchymal stem cells (MSCs) and to further elucidate the mechanotransductive roles of ..
  8. Eloe E, Malfatti F, Gutierrez J, Hardy K, Schmidt W, Pogliano K, et al. Isolation and characterization of a psychropiezophilic alphaproteobacterium. Appl Environ Microbiol. 2011;77:8145-53 pubmed publisher
    ..a deep-sea bacterium following dilution-to-extinction cultivation using a natural seawater medium at high hydrostatic pressure and low temperature...
  9. Rosenbaum E, Gabel F, Durá M, Finet S, Cléry Barraud C, Masson P, et al. Effects of hydrostatic pressure on the quaternary structure and enzymatic activity of a large peptidase complex from Pyrococcus horikoshii. Arch Biochem Biophys. 2012;517:104-10 pubmed publisher
    While molecular adaptation to high temperature has been extensively studied, the effect of hydrostatic pressure on protein structure and enzymatic activity is still poorly understood...
  10. Pribenszky C, Vajta G. Cells under pressure: how sublethal hydrostatic pressure stress treatment increases gametes' and embryos' performance. Reprod Fertil Dev. 2011;23:48-55 pubmed publisher
  11. Siqueira Filho E, Caixeta E, Pribenszky C, Molnar M, Horvath A, Harnos A, et al. Vitrification of bovine blastocysts pretreated with sublethal hydrostatic pressure stress: evaluation of post-thaw in vitro development and gene expression. Reprod Fertil Dev. 2011;23:585-90 pubmed publisher
    ..study was to evaluate the effect of different equilibration times between the termination of a sublethal hydrostatic pressure (HP) stress treatment and the initiation of vitrification on the post-thaw survival, continued in vitro ..
  12. Watanabe T, Imada K, Yoshizawa K, Nishiyama M, Kato C, Abe F, et al. Glycine insertion makes yellow fluorescent protein sensitive to hydrostatic pressure. PLoS ONE. 2013;8:e73212 pubmed publisher
    ..In response to changes in hydrostatic pressure, a spectrum shift and an intensity change of the fluorescence were observed...
  13. Stewart M, Helenius J, Toyoda Y, Ramanathan S, Muller D, Hyman A. Hydrostatic pressure and the actomyosin cortex drive mitotic cell rounding. Nature. 2011;469:226-30 pubmed publisher
    ..Thus, by locally modulating actomyosin-cortex-dependent surface tension and globally regulating osmotic pressure, cells can control their volume, shape and mechanical properties. ..
  14. Arcangeli G, Terregino C, De Benedictis P, Zecchin B, Manfrin A, Rossetti E, et al. Effect of high hydrostatic pressure on murine norovirus in Manila clams. Lett Appl Microbiol. 2012;54:325-9 pubmed publisher
    ..High hydrostatic pressure processing (HHP) is a food treatment technique that has been shown to inactivate NV...
  15. Kovac K, Bouwknegt M, Diez Valcarce M, Raspor P, Hernandez M, Rodríguez Lázaro D. Evaluation of high hydrostatic pressure effect on human adenovirus using molecular methods and cell culture. Int J Food Microbiol. 2012;157:368-74 pubmed publisher
    ..Therefore, efficient inactivation technologies for water and foods are needed. High hydrostatic pressure (HHP) processing is a non-thermal, energy-efficient and rapid emergent inactivation technology, which has ..
  16. Tang T, Brooks N, Jeworrek C, Ces O, Terrill N, Winter R, et al. Hydrostatic pressure effects on the lamellar to gyroid cubic phase transition of monolinolein at limited hydration. Langmuir. 2012;28:13018-24 pubmed publisher
    ..Here, we report on the effects of hydrostatic pressure, temperature, and water composition on the structural behavior and stability of inverse lyotropic liquid ..
  17. Brown A, Thatje S. Respiratory response of the deep-sea amphipod Stephonyx biscayensis indicates bathymetric range limitation by temperature and hydrostatic pressure. PLoS ONE. 2011;6:e28562 pubmed publisher
    Depth zonation of fauna on continental margins is well documented. Whilst increasing hydrostatic pressure with depth has long been considered a factor contributing significantly to this pattern, discussion of the relative significance of ..
  18. Dommerich S, Frickmann H, Ostwald J, Lindner T, Zautner A, Arndt K, et al. Effects of high hydrostatic pressure on bacterial growth on human ossicles explanted from cholesteatoma patients. PLoS ONE. 2012;7:e30150 pubmed publisher
    High hydrostatic pressure (HHP) treatment can eliminate cholesteatoma cells from explanted human ossicles prior to re-insertion...
  19. Kovac K, Diez Valcarce M, Raspor P, Hernandez M, Rodríguez Lázaro D. Effect of high hydrostatic pressure processing on norovirus infectivity and genome stability in strawberry puree and mineral water. Int J Food Microbiol. 2012;152:35-9 pubmed publisher
    ..Finally, we found no consistent correlation between RT-qPCR and virus infectivity results, and consequently RT-qPCR is not a satisfactory tool for predicting risks to human health. ..
  20. Jeong J, Park S, Shin J, Kang Y, Han K, Shin J. Effects of intermittent hydrostatic pressure magnitude on the chondrogenesis of MSCs without biochemical agents under 3D co-culture. J Mater Sci Mater Med. 2012;23:2773-81 pubmed publisher
    ..For the investigation a computer-controllable bioreactor was designed and used to engage intermittent hydrostatic pressure (IHP). Five different magnitudes (0.20, 0.10, 0.05, 0.02 MPa and no stimulation) of IHP were applied...
  21. Puetzer J, Williams J, Gillies A, Bernacki S, Loboa E. The effects of cyclic hydrostatic pressure on chondrogenesis and viability of human adipose- and bone marrow-derived mesenchymal stem cells in three-dimensional agarose constructs. Tissue Eng Part A. 2013;19:299-306 pubmed publisher
    This study investigates the effects of cyclic hydrostatic pressure (CHP) on chondrogenic differentiation of human adipose-derived stem cells (hASCs) in three-dimensional (3-D) agarose constructs maintained in a complete growth medium ..
  22. Liu B, Ma X, Guo D, Guo Y, Chen N, Bi H. Neuroprotective effect of alpha-lipoic acid on hydrostatic pressure-induced damage of retinal ganglion cells in vitro. Neurosci Lett. 2012;526:24-8 pubmed publisher
    ..This study aims to investigate whether ALA possesses neuroprotection against hydrostatic pressure-induced damage and explore its possible protective mechanism in cultured retinal ganglion cells (RGCs) in ..
  23. Espina L, García Gonzalo D, Laglaoui A, Mackey B, Pagán R. Synergistic combinations of high hydrostatic pressure and essential oils or their constituents and their use in preservation of fruit juices. Int J Food Microbiol. 2013;161:23-30 pubmed publisher
    ..achieved with Escherichia coli O157:H7 and Listeria monocytogenes EGD-e by combined processes of high hydrostatic pressure (HHP) and essential oils (EOs) or their chemical constituents (CCs)...
  24. Martini S, Al Ali B, Garel M, Nerini D, Grossi V, Pacton M, et al. Effects of hydrostatic pressure on growth and luminescence of a moderately-piezophilic luminous bacteria Photobacterium phosphoreum ANT-2200. PLoS ONE. 2013;8:e66580 pubmed publisher
    ..The effects of hydrostatic pressure on its growth and luminescence have been investigated under controlled laboratory conditions, using a ..
  25. Russo D, Ortore M, Spinozzi F, Mariani P, Loupiac C, Annighöfer B, et al. The impact of high hydrostatic pressure on structure and dynamics of ?-lactoglobulin. Biochim Biophys Acta. 2013;1830:4974-80 pubmed publisher
    ..scattering measurements with inelastic neutron scattering experiments, we investigated the impact of high hydrostatic pressure on the structure and dynamics of ?-lactoglobulin (?LG) in aqueous solution...
  26. Araujo T, Borges J, Ramos C, Meyer Fernandes J, Oliveira Júnior R, Pascutti P, et al. Conformational changes in human Hsp70 induced by high hydrostatic pressure produce oligomers with ATPase activity but without chaperone activity. Biochemistry. 2014;53:2884-9 pubmed publisher
    We investigated the folding of the 70 kDa human cytosolic inducible protein (Hsp70) in vitro using high hydrostatic pressure as a denaturing agent...
  27. Extrand C. Repellency of the lotus leaf: resistance to water intrusion under hydrostatic pressure. Langmuir. 2011;27:6920-5 pubmed publisher
    ..The predicted maximum pressure agrees well with reported values from experimental measurements. ..
  28. Gogal R, Kerr R, Kingsley D, Granata L, LeRoith T, Holliman S, et al. High hydrostatic pressure processing of murine norovirus 1-contaminated oysters inhibits oral infection in STAT-1(-/-)-deficient female mice. J Food Prot. 2011;74:209-14 pubmed publisher
    ..Further, the data suggest that HPP may be an effective food processing intervention for norovirus-contaminated shellfish and thus may decrease risk to both immunocompromised and immunocompetent individuals who consume shellfish. ..
  29. Neetoo H, Chen H. Individual and combined application of dry heat with high hydrostatic pressure to inactivate Salmonella and Escherichia coli O157:H7 on alfalfa seeds. Food Microbiol. 2011;28:119-27 pubmed publisher
    ..study were to comparatively assess the efficacy of dry heat treatments alone or in conjunction with high hydrostatic pressure (HHP) to eliminate a ?5 log CFU/g load of Salmonella and E. coli O157:H7 on alfalfa seeds...
  30. Oger P, Jebbar M. The many ways of coping with pressure. Res Microbiol. 2010;161:799-809 pubmed publisher
    ..biosphere, especially piezophiles (from the greek piezo = to press and philo = love), to cope with high hydrostatic pressure (HHP) prevailing in these biotopes...
  31. Leon J, Kingsley D, Montes J, Richards G, Lyon G, Abdulhafid G, et al. Randomized, double-blinded clinical trial for human norovirus inactivation in oysters by high hydrostatic pressure processing. Appl Environ Microbiol. 2011;77:5476-82 pubmed publisher
    ..In this study, we conducted a randomized, double-blinded clinical trial to assess the effect of high hydrostatic pressure processing (HPP) on Norwalk virus (HuNoV genogroup I...
  32. Li H, Zhu K, Zhou H, Peng W. Effects of high hydrostatic pressure on some functional and nutritional properties of soy protein isolate for infant formula. J Agric Food Chem. 2011;59:12028-36 pubmed publisher
    The effects and mechanism of high hydrostatic pressure (HHP) on some functional and nutritional properties of soy protein isolate (SPI) for infant formula were investigated...
  33. Ishikawa M, Yoshitomi T, Zorumski C, Izumi Y. Downregulation of glutamine synthetase via GLAST suppression induces retinal axonal swelling in a rat ex vivo hydrostatic pressure model. Invest Ophthalmol Vis Sci. 2011;52:6604-16 pubmed publisher
    ..METHODS. Ex vivo rat retinas were exposed to elevated hydrostatic pressure for 24 hours. The expression of GLAST and GS were examined using immunochemistry and real-time PCR analysis...
  34. Ogura K, Kobashigawa Y, Saio T, Kumeta H, Torikai S, Inagaki F. Practical applications of hydrostatic pressure to refold proteins from inclusion bodies for NMR structural studies. Protein Eng Des Sel. 2013;26:409-16 pubmed publisher
    Recently, the hydrostatic pressure refolding method was reported as a practical tool for solubilizing and refolding proteins from inclusion bodies; however, there have been only a few applications for protein structural studies...
  35. Vinardell T, Rolfe R, Buckley C, Meyer E, Ahearne M, Murphy P, et al. Hydrostatic pressure acts to stabilise a chondrogenic phenotype in porcine joint tissue derived stem cells. Eur Cell Mater. 2012;23:121-32; discussion 133-4 pubmed
    b>Hydrostatic pressure (HP) is a key component of the in vivo joint environment and has been shown to enhance chondrogenesis of stem cells...
  36. Black S, Dawson A, Ward F, Allen R. Genes required for growth at high hydrostatic pressure in Escherichia coli K-12 identified by genome-wide screening. PLoS ONE. 2013;8:e73995 pubmed publisher
    ..much of the global microbial biosphere is believed to exist in high pressure environments, the effects of hydrostatic pressure on microbial physiology remain poorly understood...
  37. Safshekan F, Tafazzoli Shadpour M, Shokrgozar M, Haghighipour N, Mahdian R, Hemmati A. Intermittent hydrostatic pressure enhances growth factor-induced chondroinduction of human adipose-derived mesenchymal stem cells. Artif Organs. 2012;36:1065-71 pubmed publisher
    b>Hydrostatic pressure (HP) plays an essential role in regulating function of chondrocytes and chondrogenic differentiation...
  38. Nakimbugwe D, Masschalck B, Anim G, Michiels C. Inactivation of gram-negative bacteria in milk and banana juice by hen egg white and lambda lysozyme under high hydrostatic pressure. Int J Food Microbiol. 2006;112:19-25 pubmed
    ..8 and a lytic mechanism at pH 6.8. Based on these results, LaL may offer interesting perspectives for use as an extra hurdle in high pressure food preservation. ..
  39. Guan D, Kniel K, Calci K, Hicks D, Pivarnik L, Hoover D. Response of four types of coliphages to high hydrostatic pressure. Food Microbiol. 2006;23:546-51 pubmed
    ..5-log(10) when exposed to 400 MPa for 5 min. Treatment at 450 MPa for 5 min was necessary to obtain a log(10) reduction of 6-7 for T4...
  40. Neidlinger Wilke C, Würtz K, Urban J, Börm W, Arand M, Ignatius A, et al. Regulation of gene expression in intervertebral disc cells by low and high hydrostatic pressure. Eur Spine J. 2006;15 Suppl 3:S372-8 pubmed
    Intervertebral disc structures are exposed to wide ranges of intradiscal hydrostatic pressure during different loading exercises and are at their minimum during lying or relaxed sitting and at maximum during lifting weights with a round ..
  41. Kiefer M, Meier U, Eymann R. Gravitational valves: relevant differences with different technical solutions to counteract hydrostatic pressure. Acta Neurochir Suppl. 2006;96:343-7 pubmed
    ..The frequency and severity of complications was pronounced in the DSV group. We recommend the Aesculap-Miethke-GAV valve with a low opening pressure in a posture-independent valve for patients with NPH. ..
  42. Colloc h N, Girard E, Dhaussy A, Kahn R, Ascone I, Mezouar M, et al. High pressure macromolecular crystallography: the 140-MPa crystal structure at 2.3 A resolution of urate oxidase, a 135-kDa tetrameric assembly. Biochim Biophys Acta. 2006;1764:391-7 pubmed
    ..Results suggest that the onset of disruption of the tetrameric assembly by pressure has been captured in the crystalline state. ..
  43. Chura Chambi R, Genova L, Affonso R, Morganti L. Refolding of endostatin from inclusion bodies using high hydrostatic pressure. Anal Biochem. 2008;379:32-9 pubmed publisher
    High hydrostatic pressure was used for concomitant solubilization and refolding of insoluble endostatin (ES) aggregated as inclusion bodies (IBs)...
  44. Sakao K, Takahashi K, Arai Y, Inoue A, Tonomura H, Saito M, et al. Induction of chondrogenic phenotype in synovium-derived progenitor cells by intermittent hydrostatic pressure. Osteoarthritis Cartilage. 2008;16:805-14 pubmed publisher
    The aim of this study was to investigate the effect of intermittent hydrostatic pressure (IHP) on chondrogenic differentiation of synovium-derived progenitor cells (SPCs)...
  45. Candiani G, Raimondi M, Aurora R, Laganà K, Dubini G. Chondrocyte response to high regimens of cyclic hydrostatic pressure in 3-dimensional engineered constructs. Int J Artif Organs. 2008;31:490-9 pubmed
    ..potential application in cartilage tissue engineering, only a few studies have examined the response to hydrostatic pressure of engineered constructs...
  46. Shimasaki N, Kiyohara T, Totsuka A, Nojima K, Okada Y, Yamaguchi K, et al. Inactivation of hepatitis A virus by heat and high hydrostatic pressure: variation among laboratory strains. Vox Sang. 2009;96:14-9 pubmed publisher
    ..To select an appropriate cell-adapted HAV strain for virus validation, we compared the inactivation efficiency among four strains under two different physical inactivation treatments: heat and high hydrostatic pressure.
  47. Naal F, Mengele K, Schauwecker J, Gollwitzer H, Gerdesmeyer L, Reuning U, et al. High hydrostatic pressure-induced cell death in human chondrocytes and chondrosarcoma cells. Anticancer Res. 2005;25:1977-82 pubmed
    ..In this regard, high hydrostatic pressure (HHP) treatment of bone is a new, advancing technology, now being used in preclinical testing to inactivate ..
  48. Heyland J, Wiegandt K, Goepfert C, Nagel Heyer S, Ilinich E, Schumacher U, et al. Redifferentiation of chondrocytes and cartilage formation under intermittent hydrostatic pressure. Biotechnol Lett. 2006;28:1641-8 pubmed
    Since articular cartilage is subjected to varying loads in vivo and undergoes cyclic hydrostatic pressure during periods of loading, it is hypothesized that mimicking these in vivo conditions can enhance synthesis of important matrix ..
  49. Ju W, Liu Q, Kim K, Crowston J, Lindsey J, Agarwal N, et al. Elevated hydrostatic pressure triggers mitochondrial fission and decreases cellular ATP in differentiated RGC-5 cells. Invest Ophthalmol Vis Sci. 2007;48:2145-51 pubmed
    ..The purpose of this study was to determine whether elevated hydrostatic pressure induces mitochondrial fission and dysfunction in cultured retinal ganglion cells...
  50. Tarasevicius S, Kesteris U, Gelmanas A, Smailys A, Wingstrand H. Intracapsular pressure and elasticity of the hip joint capsule in osteoarthritis. J Arthroplasty. 2007;22:596-600 pubmed
    ..The intracapsular hydrostatic pressure was measured perioperatively, and 0...
  51. Goto M, Kusube M, Tamai N, Matsuki H, Kaneshina S. Effect of hydrostatic pressure on the bilayer phase behavior of symmetric and asymmetric phospholipids with the same total chain length. Biochim Biophys Acta. 2008;1778:1067-78 pubmed publisher
  52. Gaspar L, Mendes Y, Yamamura A, Almeida L, Caride E, Gonçalves R, et al. Pressure-inactivated yellow fever 17DD virus: implications for vaccine development. J Virol Methods. 2008;150:57-62 pubmed publisher
    ..Therefore, alternative strategies should be considered, which may include inactivated whole virus. High hydrostatic pressure has been described as a method for viral inactivation and vaccine development...
  53. Padilla J, Sheldon R, Sitar D, Newcomer S. Impact of acute exposure to increased hydrostatic pressure and reduced shear rate on conduit artery endothelial function: a limb-specific response. Am J Physiol Heart Circ Physiol. 2009;297:H1103-8 pubmed publisher
    Unlike quadrupeds, humans exhibit a larger hydrostatic pressure in the lower limbs compared with the upper limbs during a major part of the day...