sindbis virus

Summary

Summary: The type species of ALPHAVIRUS normally transmitted to birds by CULEX mosquitoes in Egypt, South Africa, India, Malaya, the Philippines, and Australia. It may be associated with fever in humans. Serotypes (differing by less than 17% in nucleotide sequence) include Babanki, Kyzylagach, and Ockelbo viruses.

Top Publications

  1. pmc Autophagy protects against Sindbis virus infection of the central nervous system
    Anthony Orvedahl
    Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, 75390, USA
    Cell Host Microbe 7:115-27. 2010
  2. pmc Protection against fatal Sindbis virus encephalitis by beclin, a novel Bcl-2-interacting protein
    X H Liang
    Departments of Medicine, Columbia University College of Physicians and Surgeons, New York, New York 10032, USA
    J Virol 72:8586-96. 1998
  3. pmc Alphavirus-derived small RNAs modulate pathogenesis in disease vector mosquitoes
    Kevin M Myles
    Department of Entomology, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA
    Proc Natl Acad Sci U S A 105:19938-43. 2008
  4. pmc Suppression of RNA interference increases alphavirus replication and virus-associated mortality in Aedes aegypti mosquitoes
    Chris M Cirimotich
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    BMC Microbiol 9:49. 2009
  5. pmc Identification of interferon-stimulated gene 15 as an antiviral molecule during Sindbis virus infection in vivo
    Deborah J Lenschow
    Department of Pathology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Virol 79:13974-83. 2005
  6. pmc Adaptation of Sindbis virus to BHK cells selects for use of heparan sulfate as an attachment receptor
    W B Klimstra
    Department of Microbiology and Immunology, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA
    J Virol 72:7357-66. 1998
  7. pmc Roles of nonstructural protein nsP2 and Alpha/Beta interferons in determining the outcome of Sindbis virus infection
    Elena I Frolova
    Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas 77555 1019, USA
    J Virol 76:11254-64. 2002
  8. pmc Gamma interferon-dependent, noncytolytic clearance of sindbis virus infection from neurons in vitro
    Rebeca Burdeinick-Kerr
    W Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, 615 North Wolfe Street, Baltimore, Maryland 21205, USA
    J Virol 79:5374-85. 2005
  9. pmc The 3' untranslated region of sindbis virus represses deadenylation of viral transcripts in mosquito and Mammalian cells
    Nicole L Garneau
    Colorado State University, Department of Microbiology, Immunology and Pathology, 1682 Campus Delivery, Fort Collins, CO 80523 1682, USA
    J Virol 82:880-92. 2008
  10. pmc Recovery from viral encephalomyelitis: immune-mediated noncytolytic virus clearance from neurons
    Diane E Griffin
    W Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, Rm E5132, Baltimore, MD 21205, USA
    Immunol Res 47:123-33. 2010

Detail Information

Publications284 found, 100 shown here

  1. pmc Autophagy protects against Sindbis virus infection of the central nervous system
    Anthony Orvedahl
    Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, 75390, USA
    Cell Host Microbe 7:115-27. 2010
    ..Here, we report that the autophagy gene Atg5 function is critical for protection against lethal Sindbis virus (SIN) infection of the mouse central nervous system...
  2. pmc Protection against fatal Sindbis virus encephalitis by beclin, a novel Bcl-2-interacting protein
    X H Liang
    Departments of Medicine, Columbia University College of Physicians and Surgeons, New York, New York 10032, USA
    J Virol 72:8586-96. 1998
    bcl-2, the prototypic cellular antiapoptotic gene, decreases Sindbis virus replication and Sindbis virus-induced apoptosis in mouse brains, resulting in protection against lethal encephalitis...
  3. pmc Alphavirus-derived small RNAs modulate pathogenesis in disease vector mosquitoes
    Kevin M Myles
    Department of Entomology, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA
    Proc Natl Acad Sci U S A 105:19938-43. 2008
    ..These results suggest that an exogenous siRNA pathway is essential to the survival of mosquitoes infected with alphaviruses and, thus, the maintenance of these viruses in nature...
  4. pmc Suppression of RNA interference increases alphavirus replication and virus-associated mortality in Aedes aegypti mosquitoes
    Chris M Cirimotich
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    BMC Microbiol 9:49. 2009
    ..The goal of this study was to use a recombinant Sindbis virus (SINV; family Togaviridae; genus Alphavirus) that expresses B2 protein of Flock House virus (FHV; family ..
  5. pmc Identification of interferon-stimulated gene 15 as an antiviral molecule during Sindbis virus infection in vivo
    Deborah J Lenschow
    Department of Pathology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Virol 79:13974-83. 2005
    ..To identify additional IFN-induced antiviral molecules, we utilized a recombinant chimeric Sindbis virus to express selected ISGs in IFN-alpha/beta receptor (IFN-alpha/betaR)(-/-) mice and looked for attenuation of ..
  6. pmc Adaptation of Sindbis virus to BHK cells selects for use of heparan sulfate as an attachment receptor
    W B Klimstra
    Department of Microbiology and Immunology, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA
    J Virol 72:7357-66. 1998
    Attachment of Sindbis virus to the cell surface glycosaminoglycan heparan sulfate (HS) and the selection of this phenotype by cell culture adaptation were investigated...
  7. pmc Roles of nonstructural protein nsP2 and Alpha/Beta interferons in determining the outcome of Sindbis virus infection
    Elena I Frolova
    Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas 77555 1019, USA
    J Virol 76:11254-64. 2002
    Alphaviruses productively infect a variety of vertebrate and insect cell lines. In vertebrate cells, Sindbis virus redirects cellular processes to meet the needs of virus propagation...
  8. pmc Gamma interferon-dependent, noncytolytic clearance of sindbis virus infection from neurons in vitro
    Rebeca Burdeinick-Kerr
    W Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, 615 North Wolfe Street, Baltimore, Maryland 21205, USA
    J Virol 79:5374-85. 2005
    ..an in vitro cell culture system that will allow the study of mechanisms of IFN-gamma-mediated control of Sindbis virus (SINV) replication in neurons, we have characterized the susceptibility to SINV infection and IFN-gamma ..
  9. pmc The 3' untranslated region of sindbis virus represses deadenylation of viral transcripts in mosquito and Mammalian cells
    Nicole L Garneau
    Colorado State University, Department of Microbiology, Immunology and Pathology, 1682 Campus Delivery, Fort Collins, CO 80523 1682, USA
    J Virol 82:880-92. 2008
    The positive-sense transcripts of Sindbis virus (SINV) resemble cellular mRNAs in that they possess a 5' cap and a 3' poly(A) tail...
  10. pmc Recovery from viral encephalomyelitis: immune-mediated noncytolytic virus clearance from neurons
    Diane E Griffin
    W Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, Rm E5132, Baltimore, MD 21205, USA
    Immunol Res 47:123-33. 2010
    ..Using the model system of Sindbis virus-induced encephalomyelitis in mice, we have shown that immune-mediated clearance of infectious virus from ..
  11. pmc The zinc finger antiviral protein directly binds to specific viral mRNAs through the CCCH zinc finger motifs
    Xuemin Guo
    Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China
    J Virol 78:12781-7. 2004
    ..It specifically inhibits the replication of Moloney murine leukemia virus (MLV) and Sindbis virus (SIN) by preventing the accumulation of viral RNA in the cytoplasm...
  12. ncbi Selection and characterization of packaging cell lines for XJ-160 virus
    Wu Yang Zhu
    State Key Laboratory for Infectious Disease Prevention and Control, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 100 Yingxin Street, Beijing, China
    Intervirology 52:100-6. 2009
    ..XJ-160 virus is a mosquito-derived Sindbis-like virus isolated in China. Based on its infectious clone (pBR-XJ-160) we have developed an RNA-based vector system. In this work, we constructed packaging cell lines (PCLs) for XJ-160 virus...
  13. pmc The role of CD8(+) T cells and major histocompatibility complex class I expression in the central nervous system of mice infected with neurovirulent Sindbis virus
    T Kimura
    W Harry Feinstone Department of Molecular Microbiology and Immunology, School of Hygiene and Public Health, Johns Hopkins University, Baltimore, MD 21205, USA
    J Virol 74:6117-25. 2000
    ..the role of CD8(+) T cells infiltrating the neural parenchyma during encephalitis induced by neurovirulent Sindbis virus (NSV)...
  14. pmc Chikungunya virus nonstructural protein 2 inhibits type I/II interferon-stimulated JAK-STAT signaling
    Jelke J Fros
    Laboratory of Virology, Wageningen University, Droevendaalsesteeg 1, 6708 PB Wageningen, Netherlands
    J Virol 84:10877-87. 2010
    ..In addition, mutations in CHIKV-nsP2 (P718S) and Sindbis virus (SINV)-nsP2 (P726S) that render alphavirus replicons noncytopathic significantly reduced JAK-STAT inhibition...
  15. pmc Interaction of E2 glycoprotein with heparan sulfate is crucial for cellular infection of Sindbis virus
    Wuyang Zhu
    Department of Viral Encephalitis, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, and State Key Laboratory for Infectious Disease Prevention and Control SKLID, Beijing, China
    PLoS ONE 5:e9656. 2010
    Cell culture-adapted strains of Sindbis virus (SINV) initially attach to cells by the ability to interact with heparan sulfate (HS) through selective mutation for positively charged amino acid (aa) scattered in E2 glycoprotein (W. B...
  16. pmc Engineered lentivector targeting of dendritic cells for in vivo immunization
    Lili Yang
    Division of Biology, California Institute of Technology, 1200 E California Blvd, Pasadena, California 91125, USA
    Nat Biotechnol 26:326-34. 2008
    ..To target dendritic cells, we enveloped the lentivector with a viral glycoprotein from Sindbis virus engineered to be DC-SIGN-specific...
  17. ncbi Bax-independent inhibition of apoptosis by Bcl-XL
    E H Cheng
    Department of Neurology, Johns Hopkins School of Medicine, Baltimore, Maryland 21287 7681, USA
    Nature 379:554-6. 1996
    ..mutagenesis, we show here that the amino-acid residues critical for protection of cells by Bcl-XL against Sindbis virus-induced apoptosis are clustered within the Bcl-2-homology regions 1 and 2 (BH1 and BH2 regions)...
  18. pmc Protection from fatal viral encephalomyelitis: AMPA receptor antagonists have a direct effect on the inflammatory response to infection
    Ivorlyne P Greene
    Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 105:3575-80. 2008
    ..A neurovirulent strain of the alphavirus Sindbis virus (NSV) causes fatal encephalomyelitis associated with motor neuron death in adult C57BL/6 mice that can be ..
  19. pmc Evidence for a cytoplasmic microprocessor of pri-miRNAs
    Jillian S Shapiro
    Department of Microbiology, Mount Sinai School of Medicine, New York, New York 10029, USA
    RNA 18:1338-46. 2012
    ..Taken together, this research describes a unique function for Drosha in the processing of highly structured cytoplasmic RNAs in the context of virus infection...
  20. ncbi Glutamate receptor antagonists protect from virus-induced neural degeneration
    Jennifer L Nargi-Aizenman
    W Harry Feinstone Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD 21205, USA
    Ann Neurol 55:541-9. 2004
    ..in fatal alphavirus-induced paralytic encephalomyelitis, we treated mice infected with neuroadapted Sindbis virus (NSV) with antagonists of N-methyl-D-aspartate (NMDA) and alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic ..
  21. pmc Resistance of interleukin-1beta-deficient mice to fatal Sindbis virus encephalitis
    X H Liang
    Department of Medicine, Columbia University College of Physicians and Surgeons, New York, New York 10032, USA
    J Virol 73:2563-7. 1999
    ..of IL-1beta-deficient and wild-type 129 SV(ev) mice infected with a neurovirulent viral strain, neuroadapted Sindbis virus (NSV)...
  22. pmc Sindbis virus conformational changes induced by a neutralizing anti-E1 monoclonal antibody
    Raquel Hernandez
    Department of Molecular and Structural Biochemistry, North Carolina State University, Raleigh, NC 27608, USA
    J Virol 82:5750-60. 2008
    A rare Sindbis virus anti-E1 neutralizing monoclonal antibody, Sin-33, was investigated to determine the mechanism of in vitro neutralization...
  23. ncbi Larval environmental stress alters Aedes aegypti competence for Sindbis virus
    Ephantus J Muturi
    Illinois Natural History Survey, University of Illinois, Champaign, IL, USA
    Trop Med Int Health 16:955-64. 2011
    ..To evaluate how stress at the larval stage alters adult mosquito performance and susceptibility to viral infection...
  24. pmc Natural resistance-associated macrophage protein is a cellular receptor for sindbis virus in both insect and mammalian hosts
    Patrick P Rose
    Department of Microbiology, University of Pennsylvania, Philadelphia, USA
    Cell Host Microbe 10:97-104. 2011
    Alphaviruses, including several emerging human pathogens, are a large family of mosquito-borne viruses with Sindbis virus being a prototypical member of the genus...
  25. pmc Pathogenesis of encephalitis induced in newborn mice by virulent and avirulent strains of Sindbis virus
    L A Sherman
    Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205
    J Virol 64:2041-6. 1990
    Strains of Sindbis virus differ in their virulence for mice of different ages; this variation is related in large part to variations in the amino acid compositions of E1 and E2, the surface glycoproteins...
  26. pmc A single mutation in the E2 glycoprotein important for neurovirulence influences binding of sindbis virus to neuroblastoma cells
    Peiyu Lee
    W Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland 21205, USA
    J Virol 76:6302-10. 2002
    The amino acid at position 55 of the E2 glycoprotein (E2(55)) of Sindbis virus (SV) is a critical determinant of SV neurovirulence in mice...
  27. ncbi Development of a Sindbis virus expression system that efficiently expresses green fluorescent protein in midguts of Aedes aegypti following per os infection
    K E Olson
    Arthropod Borne and Infectious Diseases Laboratory, Department of Microbiology, Colorado State University, Ft Collins, CO 80523, USA
    Insect Mol Biol 9:57-65. 2000
    ..The MRE3'2 J dsSIN expression system should be an important tool for efficient gene expression in Ae. aegypti midguts...
  28. pmc Interactions of the cytoplasmic domain of Sindbis virus E2 with nucleocapsid cores promote alphavirus budding
    Joyce Jose
    Markey Center for Structural Biology, Department of Biological Sciences, Purdue University, West Lafayette, Indiana, USA
    J Virol 86:2585-99. 2012
    ..Structural studies of the Sindbis virus capsid protein (CP) have suggested that these critical interactions are mediated by the binding of cdE2 into a ..
  29. ncbi Infection of cells by Sindbis virus at low temperature
    Gongbo Wang
    Department of Molecular and Structural Biochemistry, North Carolina State University, Raleigh, NC 27695, USA
    Virology 362:461-7. 2007
    b>Sindbis virus, which belongs to the family Togaviridae genus Alphavirus infects a variety of vertebrate and invertebrate cells. The initial steps of Sindbis virus infection involve attachment, penetration and uncoating...
  30. pmc Exposure to low pH is not required for penetration of mosquito cells by Sindbis virus
    R Hernandez
    Department of Molecular and Structural Biochemistry, North Carolina State University, Raleigh, North Carolina 27695, USA
    J Virol 75:2010-3. 2001
    ..The alphavirus Sindbis virus replicates in both vertebrate and invertebrate cell cultures...
  31. pmc Sindbis virus-induced neuronal death is both necrotic and apoptotic and is ameliorated by N-methyl-D-aspartate receptor antagonists
    J L Nargi-Aizenman
    W. Harry Feinstone Department of Molecular Microbiology and Immunology, School of Hygiene and Public Health, Johns Hopkins University, Baltimore, Maryland 21205, USA
    J Virol 75:7114-21. 2001
    ..b>Sindbis virus (SV) is an alphavirus that induces apoptotic cell death both in vitro and in vivo...
  32. pmc Cis-acting RNA elements at the 5' end of Sindbis virus genome RNA regulate minus- and plus-strand RNA synthesis
    I Frolov
    Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston 77555-1019, USA
    RNA 7:1638-51. 2001
    ..In addition to providing new insight into the initiation of alphavirus genome replication, these results have implications for the development of improved alphavirus vector systems with reduced recombination potential...
  33. pmc The nsP3 macro domain is important for Sindbis virus replication in neurons and neurovirulence in mice
    Eunhye Park
    W Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health and Cellular and Molecular Medicine Graduate Program, Johns Hopkins Medical Institutions, Baltimore, MD 21205, USA
    Virology 388:305-14. 2009
    b>Sindbis virus (SINV), the prototype alphavirus, contains a macro domain in the highly conserved N-terminal region of nonstructural protein 3 (nsP3). However, the biological role of the macro domain is unclear...
  34. pmc A determinant of Sindbis virus neurovirulence enables efficient disruption of Jak/STAT signaling
    Jason D Simmons
    Department of Genetics, The Carolina Vaccine Institute, University of North Carolina at Chapel Hill, 9039 Burnett Womack Bldg, CB 7292, Chapel Hill, NC 27599, USA
    J Virol 84:11429-39. 2010
    Previous studies with Venezuelan equine encephalitis virus and Sindbis virus (SINV) indicate that alphaviruses are capable of suppressing the cellular response to type I and type II interferons (IFNs) by disrupting Jak/STAT signaling; ..
  35. pmc Host factors associated with the Sindbis virus RNA-dependent RNA polymerase: role for G3BP1 and G3BP2 in virus replication
    Ileana M Cristea
    Department of Molecular Biology, Princeton University, Lewis Thomas Laboratory, Washington Road, Princeton, New Jersey 08544, USA
    J Virol 84:6720-32. 2010
    b>Sindbis virus (SINV) is the prototype member of the Alphavirus genus, whose members cause severe human diseases for which there is no specific treatment...
  36. ncbi Individual expression of sindbis virus glycoproteins. E1 alone promotes cell fusion
    Miguel Angel Sanz
    Centro de Biologia Molecular CSIC UAM, Universidad Autonoma, Cantoblanco, 28049 Madrid, Spain
    Virology 305:463-72. 2003
    ..each glycoprotein in the absence of the other counterpart was achieved by means of electroporation of modified Sindbis virus (SV) genomes. In addition, in trans coexpression of both glycoproteins was also tested in BHK cells...
  37. ncbi A luciferase-based screening method for inhibitors of alphavirus replication applied to nucleoside analogues
    Leena Pohjala
    Program in Cellular Biotechnology, Institute of Biotechnology, P O Box 56 Viikinkaari 9, University of Helsinki, 00014 Helsinki, Finland
    Antiviral Res 78:215-22. 2008
    ..inhibitors of Semliki Forest virus (SFV) replication, and demonstrate the effects of 29 nucleosides on SFV and Sindbis virus replication...
  38. pmc Selection of functional 5' cis-acting elements promoting efficient sindbis virus genome replication
    Rodion Gorchakov
    Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas 77555 1019, USA
    J Virol 78:61-75. 2004
    The 5' portion of the Sindbis virus (SIN) genome RNA is multifunctional...
  39. pmc The cellular antiviral protein viperin is attenuated by proteasome-mediated protein degradation in Japanese encephalitis virus-infected cells
    Yi Lin Chan
    Graduate Institute of Life Sciences, National Defense Medical Center, Institute of Biomedical Sciences, Taipei, Taiwan, Republic of China
    J Virol 82:10455-64. 2008
    ..this study, we found that viperin is highly induced at the RNA level by Japanese encephalitis virus (JEV) and Sindbis virus (SIN) and that viperin protein is degraded in JEV-infected cells through a proteasome-dependent mechanism...
  40. pmc Amino acid substitutions in the E2 glycoprotein of Sindbis-like virus XJ-160 confer the ability to undergo heparan sulfate-dependent infection of mouse embryonic fibroblasts
    Wuyang Zhu
    State Key Laboratory for Infectious Disease Prevention and Control SKLID, Institute for Viral Disease Control and Prevention, China CDC, Beijing, China
    Virol J 7:225. 2010
    We have recently demonstrated an essential role of the domain of 145-150 amino acid in the E2 glycoprotein of Sindbis virus in the interaction with cellular heparan sulfate (HS) and in the infection of mouse embryonic fibroblasts (MEF) ..
  41. pmc Redirecting lentiviral vectors pseudotyped with Sindbis virus-derived envelope proteins to DC-SIGN by modification of N-linked glycans of envelope proteins
    Kouki Morizono
    Department of Medicine, David Geffen School of Medicine, University of California, Los Angeles, BSRB 173, Charles E Young Dr South, Los Angeles, CA 90095, USA
    J Virol 84:6923-34. 2010
    ..We previously developed targeting lentiviral vectors by pseudotyping with modified Sindbis virus envelope proteins...
  42. ncbi Translation driven by picornavirus IRES is hampered from Sindbis virus replicons: rescue by poliovirus 2A protease
    Miguel Angel Sanz
    Centro de Biologia Molecular Severo Ochoa, CSIC UAM, C Nicolás Cabrera, 1, Universidad Autonoma, Cantoblanco, 28049 Madrid, Spain
    J Mol Biol 402:101-17. 2010
    ..We have investigated the translatability of Sindbis virus (SV) subgenomic mRNA bearing different 5'-untranslated regions, including several viral internal ribosome ..
  43. ncbi Substitutions of 169Lys and 173Thr in nonstructural protein 1 influence the infectivity and pathogenicity of XJ-160 virus
    Wu Yang Zhu
    State Key Laboratory for Infectious Disease Control and Prevention, Institute for Viral Disease Control and Prevention, China CDC, 100 Yingxin Street, Xuan Wu District, 100052 Beijing, China
    Arch Virol 154:245-53. 2009
    ..These mutations might be associated with the RNA methyltransferase (MTase) activity of nsP1...
  44. pmc Sindbis viral vector induced apoptosis requires translational inhibition and signaling through Mcl-1 and Bak
    Lisa Venticinque
    NYU Cancer Institute and the NYU Gene Therapy Center, NYU School of Medicine, 550 First Avenue, New York, NY 10016, USA
    Mol Cancer 9:37. 2010
    ..A comprehensive understanding of the mechanism of apoptosis would facilitate the design of more effective vectors for cancer therapy...
  45. ncbi Interferon-gamma-mediated site-specific clearance of alphavirus from CNS neurons
    G K Binder
    W Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins University Bloomberg School of Public Health, Baltimore, MD 21205, USA
    Science 293:303-6. 2001
    ..To understand the role of T cells in clearance, we infected antibody knockout mice with Sindbis virus. Virus was cleared from spinal cord and brain stem neurons, but not from cortical neurons, and required both ..
  46. pmc Genetic determinants of Sindbis virus mosquito infection are associated with a highly conserved alphavirus and flavivirus envelope sequence
    Dennis J Pierro
    Arthropod Borne and Infectious Disease Laboratory, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado 80523, USA
    J Virol 82:2966-74. 2008
    Wild-type Sindbis virus (SINV) strain MRE16 efficiently infects Aedes aegypti midgut epithelial cells (MEC), but laboratory-derived neurovirulent SINV strain TE/5'2J infects MEC poorly...
  47. pmc The zinc finger antiviral protein acts synergistically with an interferon-induced factor for maximal activity against alphaviruses
    Margaret R Macdonald
    Laboratory of Virology and Infectious Disease, The Rockefeller University, Box 64, 1230 York Avenue, New York, NY 10065, USA
    J Virol 81:13509-18. 2007
    ..Since ZAP expression is induced by IFN, we utilized Sindbis virus (SINV) to investigate the role of other IFN-induced factors in ZAP's inhibitory potential...
  48. pmc Sindbis virus usurps the cellular HuR protein to stabilize its transcripts and promote productive infections in mammalian and mosquito cells
    Kevin J Sokoloski
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523 1682, USA
    Cell Host Microbe 8:196-207. 2010
    ..We demonstrate that Sindbis virus, a prototypical single-stranded arthropod-borne alphavirus, uses U-rich 3' UTR sequences in its RNAs to ..
  49. pmc Identification of a dominant negative inhibitor of human zinc finger antiviral protein reveals a functional endogenous pool and critical homotypic interactions
    Lok Man J Law
    Laboratory of Virology and Infectious Disease, The Rockefeller University, 1230 York Ave, New York, NY 10065, USA
    J Virol 84:4504-12. 2010
    ..ZAP blocks replication of the prototype alphavirus Sindbis virus (SINV) at a step at or before translation of the incoming viral genome...
  50. pmc Characterization of purified Sindbis virus nsP4 RNA-dependent RNA polymerase activity in vitro
    Jon K Rubach
    Department of Biological Chemistry, Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109, USA
    Virology 384:201-8. 2009
    The Sindbis virus RNA-dependent RNA polymerase (nsP4) is responsible for the replication of the viral RNA genome. In infected cells, nsP4 is localized in a replication complex along with the other viral non-structural proteins...
  51. pmc A versatile targeting system with lentiviral vectors bearing the biotin-adaptor peptide
    Kouki Morizono
    Department of Medicine, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA
    J Gene Med 11:655-63. 2009
    ..We have now developed a more stable conjugation strategy utilizing the interaction between avidin and biotin...
  52. pmc Aedes aegypti uses RNA interference in defense against Sindbis virus infection
    Corey L Campbell
    Arthropod borne Infectious Diseases Laboratory Microbiology, Immunology, and Pathology Department, Colorado State University, Fort Collins, USA
    BMC Microbiol 8:47. 2008
    ..The goal of this study was to use Ae. aegypti as a model system to determine how the mosquito's anti-viral RNAi pathway interacts with recombinant Sindbis virus (SINV; family Togaviridae, genus Alphavirus).
  53. pmc Placement of the structural proteins in Sindbis virus
    Wei Zhang
    Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907 1392, USA
    J Virol 76:11645-58. 2002
    The structure of the lipid-enveloped Sindbis virus has been determined by fitting atomic resolution crystallographic structures of component proteins into an 11-A resolution cryoelectron microscopy map...
  54. ncbi Tumor-specific in vivo transfection with HSV-1 thymidine kinase gene using a Sindbis viral vector as a basis for prodrug ganciclovir activation and PET
    Jen Chieh Tseng
    NYU Cancer Institute, Rita J and Stanley H Kaplan Comprehensive Cancer Center, NYU Gene Therapy Center, NYU School of Medicine, New York, New York 10016, USA
    J Nucl Med 47:1136-43. 2006
    ..b>Sindbis virus is an oncolytic, alpha-virus that selectively targets tumors through the 67-kDa laminin receptor (LAMR)...
  55. ncbi Inhibition of virus-induced neuronal apoptosis by Bax
    J Lewis
    Department of Pharmacology and Molecular Sciences, Johns Hopkins University Schools of Medicine and Public Health, Baltimore, Maryland 21205, USA
    Nat Med 5:832-5. 1999
    ..However, Bax was found here to be a potent inhibitor of neuronal cell death in mice infected with Sindbis virus. Newborn mice, which are highly susceptible to a fatal infection with neurotropic Sindbis virus, were ..
  56. ncbi Sindbis virus induces transport processes and alters expression of innate immunity pathway genes in the midgut of the disease vector, Aedes aegypti
    Heather R Sanders
    Department of Cell Biology and Neuroscience, University of California, Riverside, California 92521, USA
    Insect Biochem Mol Biol 35:1293-307. 2005
    ..In addition to these observations, transcript changes were observed in infected mosquitoes that suggested involvement of Toll and c-jun amino terminal kinase immune cascades as a response to viral infection...
  57. pmc Mitochondrial bioenergetic alterations in mouse neuroblastoma cells infected with Sindbis virus: implications to viral replication and neuronal death
    Leandro Silva da Costa
    Instituto de Bioquimica Medica, Universidade Federal do Rio de Janeiro, Cidade Universitaria, Rio de Janeiro, Rio de Janeiro, Brazil
    PLoS ONE 7:e33871. 2012
    ..b>Sindbis virus, the prototype of and most widespread alphavirus, causes outbreaks of arthritis in humans and serves as a ..
  58. pmc Effects of manipulating apoptosis on Sindbis virus infection of Aedes aegypti mosquitoes
    Hua Wang
    Division of Biology, Kansas State University, Manhattan, Kansas, USA
    J Virol 86:6546-54. 2012
    ..When mosquitoes which had been injected with Aeiap1 double-stranded RNA (dsRNA) were orally infected with Sindbis virus (SINV), increased midgut infection and virus dissemination to other organs were observed...
  59. pmc Role of sindbis virus capsid protein region II in nucleocapsid core assembly and encapsidation of genomic RNA
    Ranjit Warrier
    Markey Center for Structural Biology, Department of Biological Sciences, Purdue University, 915 W State St, West Lafayette, IN 47907
    J Virol 82:4461-70. 2008
    b>Sindbis virus is an enveloped positive-sense RNA virus in the alphavirus genus. The nucleocapsid core contains the genomic RNA surrounded by 240 copies of a single capsid protein...
  60. ncbi Enhancement of Sindbis virus self-replicating RNA vaccine potency by linkage of Mycobacterium tuberculosis heat shock protein 70 gene to an antigen gene
    W F Cheng
    Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, MD 21205, USA
    J Immunol 166:6218-26. 2001
    ..Mycobacterium tuberculosis heat shock protein 70 (HSP70) on the potency of Ag-specific immunity generated by a Sindbis virus self-replicating RNA vector, SINrep5...
  61. pmc Inhibition of host translation by virus infection in vivo
    René Toribio
    Departamento de Biologia Molecular and Centro de Biologia Molecular Severo Ochoa, Consejo Superior de Investigaciones Cientificas Universidad Autonoma de Madrid, Universidad Autonoma de Madrid, Cantoblanco 28049 Madrid, Spain
    Proc Natl Acad Sci U S A 107:9837-42. 2010
    ..To address this issue, we constructed recombinant Sindbis virus (SV)-expressing reporter mRNA, the translation of which is sensitive or resistant to virus-induced shut off...
  62. ncbi Climatic controls on West Nile virus and Sindbis virus transmission and outbreaks in South Africa
    Christopher K Uejio
    Research Applications Laboratory, National Center for Atmospheric Research, 3090 Center Green Drive, Boulder, CO 80301, USA
    Vector Borne Zoonotic Dis 12:117-25. 2012
    ..South Africa has a long WNV and Sindbis virus (SINV) record where consistent climate and disease relationships can be identified...
  63. pmc Oncolytic Sindbis virus targets tumors defective in the interferon response and induces significant bystander antitumor immunity in vivo
    Pong Yu Huang
    Graduate Institute of Microbiology, National Taiwan University College of Medicine, Taipei, Taiwan
    Mol Ther 20:298-305. 2012
    b>Sindbis virus (SBV) has been shown to possess oncolytic potential in many human xenograft tumor models in immunocompromised mice. However, the mechanism underlying the tumor selectivity of SBV remains undetermined...
  64. ncbi Altered behavioral responses of Sindbis virus-infected Aedes aegypti (Diptera: Culicidae) to DEET and non-DEET based insect repellents
    Whitney A Qualls
    Anastasia Mosquito Control District, St Augustine, FL, United States
    Acta Trop 122:284-90. 2012
    ..mosquitoes following dissemination of Sindbis virus (SINV) were observed after exposure to repellents with the active ingredients (AI) DEET, picaridin, 2-..
  65. pmc Effects of inducing or inhibiting apoptosis on Sindbis virus replication in mosquito cells
    Hua Wang
    Molecular, Cellular, and Developmental Biology Program, Arthropod Genomics Center, Division of Biology, Kansas State University, Manhattan, KS 66506, USA
    J Gen Virol 89:2651-61. 2008
    b>Sindbis virus (SINV) is a mosquito-borne virus in the genus Alphavirus, family Togaviridae...
  66. pmc An improved in vitro and in vivo Sindbis virus expression system through host and virus engineering
    Toey Nivitchanyong
    Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD, United States
    Virus Res 141:1-12. 2009
    ..Limiting the lethality inherent in the Sindbis virus vector in order to enable long term, sustained expression of recombinant proteins may be possible...
  67. pmc Synthesis of dioxane-based antiviral agents and evaluation of their biological activities as inhibitors of Sindbis virus replication
    Ha Young Kim
    Department of Medicinal Chemistry and Molecular Pharmacology, School of Pharmacy and Pharmaceutical Sciences, The Purdue Cancer Center, West Lafayette, IN 47907, USA
    Bioorg Med Chem 15:2667-79. 2007
    The crystal structure of the Sindbis virus capsid protein contains one or two solvent-derived dioxane molecules in the hydrophobic binding pocket...
  68. ncbi Sindbis virus induced phosphorylation of IRF3 in human embryonic kidney cells is not dependent on mTOR
    Nisha R Dhanushkodi
    Department of Microbiology and Immunology, Meharry Medical College, School of Medicine, Nashville, Tennessee, USA
    Innate Immun 18:325-32. 2012
    ..We previously showed that infection of human embryonic kidney (HEK) cells with Sindbis virus (SIN), an enveloped RNA alphavirus, profoundly suppresses Akt/mTOR signaling, and host translation late during ..
  69. pmc Cell-specific targeting of lentiviral vectors mediated by fusion proteins derived from Sindbis virus, vesicular stomatitis virus, or avian sarcoma/leukosis virus
    Xian Yang Zhang
    Gene Therapy Program, Department of Medicine, Louisiana State University Health Sciences Center, New Orleans, Louisiana 70112, USA
    Retrovirology 7:3. 2010
    ..particles bearing a membrane-bound stem cell factor sequence plus a separate fusion protein based either on Sindbis virus strain TR339 glycoproteins or the vesicular stomatitis virus G glycoprotein were used to selectively transduce ..
  70. pmc Control of Sindbis virus infection by antibody in interferon-deficient mice
    A P Byrnes
    Department of Molecular Microbiology, Johns Hopkins University School of Hygiene and Public Health, Baltimore, Maryland 21205, USA
    J Virol 74:3905-8. 2000
    Antibodies clear Sindbis virus from infected animals through an unknown mechanism...
  71. pmc Nucleic acid-dependent cross-linking of the nucleocapsid protein of Sindbis virus
    T L Tellinghuisen
    Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907, USA
    J Virol 74:4302-9. 2000
    ..Through the use of both truncated and mutant Sindbis virus nucleocapsid proteins and a variety of cross-linking reagents, a possible nucleic acid-protein assembly ..
  72. pmc A new role for ns polyprotein cleavage in Sindbis virus replication
    Rodion Gorchakov
    Department of Microbiology and Immunology, University of Texas Medical Branch, 301 University Boulevard, Galveston, TX 77555 1019, USA
    J Virol 82:6218-31. 2008
    ..In this study, we developed Sindbis virus mutants that were incapable of either P23 or P123 cleavage...
  73. ncbi Design, synthesis, and evaluation of dioxane-based antiviral agents targeted against the Sindbis virus capsid protein
    Ha Young Kim
    Department of Medicinal Chemistry and Molecular Pharmacology, The Purdue Cancer Center, School of Pharmacy and Pharmaceutical Sciences, Purdue University, West Lafayette, IN 47907, USA
    Bioorg Med Chem Lett 15:3207-11. 2005
    Dioxane-based antiviral agents targeted to the hydrophobic binding pocket of Sindbis virus capsid protein were designed by computer graphics molecular modeling and synthesized...
  74. ncbi Monoclonal antibodies to the E1 and E2 glycoproteins of Sindbis virus: definition of epitopes and efficiency of protection from fatal encephalitis
    Q P Mendoza
    Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205
    J Gen Virol 69:3015-22. 1988
    Protection of mice from fatal neuroadapted Sindbis virus encephalitis can be accomplished by passive transfer of monoclonal antibodies (MAbs) to either the E1 or E2 glycoprotein of Sindbis virus...
  75. ncbi Sindbis virus as a model for studies of conformational changes in a metastable virus and the role of conformational changes in in vitro antibody neutralisation
    Raquel Hernandez
    Department of Molecular and Structural Biochemistry, North Carolina State University, Raleigh, NC 27695 7622, USA
    Rev Med Virol 19:257-72. 2009
    b>Sindbis virus has long been the prototypical alphavirus used in the study of assembly of icosahedral viruses which contain membranes...
  76. ncbi Mutations in the Sindbis virus capsid gene can partially suppress mutations in the cytoplasmic domain of the virus E2 glycoprotein spike
    C Ryan
    Department of Molecular Microbiology, Washington University School of Medicine, St Louis, Missouri 63110 1093, USA
    Virology 243:380-7. 1998
    ..Genetic data to support this model have been obtained by isolating revertants of two slow-growth mutants of Sindbis virus and analyzing the sequences of the genes encoding their structural proteins...
  77. pmc Sindbis virus nucleocapsid assembly: RNA folding promotes capsid protein dimerization
    Benjamin R Linger
    Department of Biochemistry and Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907, USA
    RNA 10:128-38. 2004
    In Sindbis virus, initiation of nucleocapsid core assembly begins with recognition of the encapsidation signal of the viral RNA genome by capsid protein...
  78. ncbi vFLIP protects PC-12 cells from apoptosis induced by Sindbis virus: implications for the role of TNF-alpha
    R Sarid
    Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, Israel 52900
    Cell Death Differ 8:1224-31. 2001
    b>Sindbis virus (SV) is an alphavirus used as a model for studying the pathogenesis of viral encephalitis...
  79. pmc A heterologous coiled coil can substitute for helix I of the Sindbis virus capsid protein
    Rushika Perera
    Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907, USA
    J Virol 77:8345-53. 2003
    ..This sequence, which spans residues 38 to 55 of the Sindbis virus capsid protein, was implicated in stabilizing dimeric contacts initiated through the C-terminal two-thirds of ..
  80. ncbi Sindbis virus replicon particles encoding calreticulin linked to a tumor antigen generate long-term tumor-specific immunity
    W F Cheng
    Department of Obstetrics and Gynecology, National Taiwan University Hospital, Taipei
    Cancer Gene Ther 13:873-85. 2006
    ..We concluded that the CRT strategy used in the context of SIN replicon particles facilitated the generation of a highly effective vaccine for cancer prophylaxis and immunotherapy...
  81. pmc IFN-stimulated gene 15 functions as a critical antiviral molecule against influenza, herpes, and Sindbis viruses
    Deborah J Lenschow
    Department of Pathology and Immunology, Washington University School of Medicine, St Louis, MO 63110, USA
    Proc Natl Acad Sci U S A 104:1371-6. 2007
    ..increased susceptibility to both herpes simplex virus type 1 and murine gammaherpesvirus 68 infection and to Sindbis virus infection...
  82. ncbi Single and multiple deletions in the transmembrane domain of the Sindbis virus E2 glycoprotein identify a region critical for normal virus growth
    Christopher B Whitehurst
    Department of Molecular and Structural Biochemistry, North Carolina State University, Raleigh, 128 Polk Hall, NC 27695, USA
    Virology 347:199-207. 2006
    b>Sindbis virus is composed of two nested T = 4 icosahedral protein shells containing 240 copies each of three structural proteins: E1, E2, and Capsid in a 1:1:1 stoichiometric ratio...
  83. ncbi Identity of Karelian fever and Ockelbo viruses determined by serum dilution-plaque reduction neutralization tests and oligonucleotide mapping
    D K Lvov
    D I Ivanovsky Institute of Virology, USSR Academy of Medical Sciences, Moscow
    Am J Trop Med Hyg 39:607-10. 1988
    ..Finland, and Karelian fever in the USSR have been attributed to alphaviruses (family Togaviridae) related to Sindbis virus. We compared prototypes Sindbis, Ockelbo, and Karelian fever viruses by neutralization tests...
  84. ncbi [Molecular basis of one-way serological reaction between SINV and XJ-160 virus]
    Li Hua Wang
    Department of Viral Encephalitis, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention IVDC, China CDC and State Key Laboratory for Infectious Disease Prevention and Control SKLID, Beijing 100052, China
    Bing Du Xue Bao 26:228-33. 2010
    ..Our study identified the SINE2 gene was the determined gene for one way serological reaction between XJ-160 virus and SINV, and this research laid the foundation for further analysis of the genomic structure and function of SINV...
  85. ncbi Sindbis virus vectors for expression in animal cells
    H V Huang
    Department of Molecular Microbiology, Washington University School of Medicine, Box 8230, 660 South Euclid Avenue, St Louis, MO 63110 1093, USA
    Curr Opin Biotechnol 7:531-5. 1996
    b>Sindbis virus and other alphavirus gene expression vectors have recently been used to express and study the functions of proteins and RNA, to evaluate classical vaccine and novel antiviral approaches, and for nucleic acid immunization...
  86. ncbi Susceptibility of colorectal cancer cells to Sindbis virus infection
    Chao Li
    Department of Pathology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73190, USA
    J Exp Ther Oncol 8:167-75. 2009
    b>Sindbis virus (SIN), a member of the Togaviridae family, infects a broad range of cells and has been shown to be an effective anti-tumor agent. The infection efficiency of the virus, however, varies greatly among target cells...
  87. ncbi Non-pathogenic Sindbis virus causes hemorrhagic fever in the absence of alpha/beta and gamma interferons
    Kate D Ryman
    Center for Molecular and Tumor Virology, Louisiana State University Health Sciences Center, Shreveport, LA 71130 3932, USA
    Virology 368:273-85. 2007
    ..We examined the contribution of IFNgamma to the protection of adult mice from Sindbis virus (SB)-induced disease by comparing subcutaneous infection of mice lacking receptors for either IFNalpha/beta (..
  88. ncbi Restricted tissue tropism and acquired resistance to Sindbis viral vector expression in the absence of innate and adaptive immunity
    J C Tseng
    NYU Cancer Institute, Department of Pathology, NYU Gene Therapy Center, NYU School of Medicine, New York University, New York, NY, USA
    Gene Ther 14:1166-74. 2007
    ..Thus it appears that in vivo, in contrast to the ready transduction of tumor cells, transduction of normal cells by replication-defective Sindbis vector is limited, possibly by a novel mechanism...
  89. ncbi Oncolytic viral therapy for cervical and ovarian cancer cells by Sindbis virus AR339 strain
    Youichi Unno
    Department of Reproductive Medicine, Graduate School of Medicine, Chiba University, Inohana, Chiba, Japan
    Clin Cancer Res 11:4553-60. 2005
    Recently, the application of replication-competent viruses has been studied as anticancer agents. Sindbis virus (SIN) is an RNA virus that belongs to the Alphavirus genus in the Togaviridae virus family...
  90. pmc Deletions in the transmembrane domain of a sindbis virus glycoprotein alter virus infectivity, stability, and host range
    Raquel Hernandez
    Department of Molecular and Structural Biochemistry, North Carolina State University, Raleigh, North Carolina 27695, USA
    J Virol 77:12710-9. 2003
    ..We also found that membrane composition effects protein-protein and protein-membrane interactions during virus assembly...
  91. ncbi Structural and functional analyses of stem-loop 1 of the Sindbis virus genome
    David G Nickens
    Department of Biology, Indiana University, 1001 E Third Street, Bloomington, IN 47405, USA
    Virology 370:158-72. 2008
    Alphavirus genome function is controlled by elements at both the 5' and 3' ends. The 5' 220 nt of the Sindbis virus genome is predicted to consist of four stem-loop structures the first of which has been demonstrated to be required for ..
  92. pmc Mutations in the endodomain of Sindbis virus glycoprotein E2 define sequences critical for virus assembly
    John West
    Department of Molecular and Structural Biochemistry, North Carolina State University, Campus Box 7622, Raleigh, North Carolina 27695 7622, USA
    J Virol 80:4458-68. 2006
    Envelopment of Sindbis virus at the plasma membrane is a multistep process in which an initial step is the association of the E2 protein via a cytoplasmic endodomain with the preassembled nucleocapsid...
  93. pmc Recombination-ready Sindbis replicon expression vectors for transgene expression
    Brian J Geiss
    Arthropod Borne and Infectious Diseases Laboratory, Department of Molecular Biology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    Virol J 4:112. 2007
    ....
  94. ncbi Pro-inflammatory response resulting from sindbis virus infection of human macrophages: implications for the pathogenesis of viral arthritis
    Iranaia Assunção-Miranda
    Program of Structural Biology, Institute of Medical Biochemistry, Federal University of Rio de Janeiro, RJ, Brazil
    J Med Virol 82:164-74. 2010
    ..b>Sindbis virus (SinV), a member of the Alphavirus genus, is the most widely distributed of all known arboviruses...
  95. pmc Noncytolytic clearance of sindbis virus infection from neurons by gamma interferon is dependent on Jak/STAT signaling
    Rebeca Burdeinick-Kerr
    W Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, 615 North Wolfe Street, Baltimore, MD 21205, USA
    J Virol 83:3429-35. 2009
    The alphavirus Sindbis virus (SINV) causes encephalomyelitis in mice by infecting neurons of the brain and spinal cord. The outcome is age dependent. Young animals develop fatal disease, while older animals recover from infection...
  96. ncbi Contribution of T cells to mortality in neurovirulent Sindbis virus encephalomyelitis
    Jennifer F Rowell
    W Harry Feinstone Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, 615 N Wolfe Street, Baltimore, MD 21205, USA
    J Neuroimmunol 127:106-14. 2002
    Intranasal inoculation of C57BL/6 mice with a neurovirulent strain of Sindbis virus (SV) results in fatal encephalomyelitis...
  97. pmc Random insertion mutagenesis of sindbis virus nonstructural protein 2 and selection of variants incapable of downregulating cellular transcription
    Ilya Frolov
    Department of Microbiology, University of Alabama at Birmingham, 1530 Third Avenue South, BBRB 373 Box 3 Birmingham, AL 53294 2170, USA
    J Virol 83:9031-44. 2009
    b>Sindbis virus nonstructural protein 2 (SINV nsP2) is an important determinant of virus pathogenesis and downregulation of virus-induced cell response...
  98. pmc Different types of nsP3-containing protein complexes in Sindbis virus-infected cells
    Rodion Gorchakov
    Department of Microbiology and Immunology, University of Texas Medical Branch, 301 University Boulevard, Galveston, TX 77555 1019, USA
    J Virol 82:10088-101. 2008
    ..The results of our study suggest that Sindbis virus (SINV) infection in BHK-21 cells leads to the formation of at least two types of nsP3-containing complexes, ..
  99. pmc Dual mechanism for the translation of subgenomic mRNA from Sindbis virus in infected and uninfected cells
    Miguel Angel Sanz
    Centro de Biologia Molecular Severo Ochoa CSIC UAM, Universidad Autonoma de Madrid, Cantoblanco, Madrid, Spain
    PLoS ONE 4:e4772. 2009
    Infection of BHK cells by Sindbis virus (SV) gives rise to a profound inhibition of cellular protein synthesis, whereas translation of viral subgenomic mRNA that encodes viral structural proteins, continues for hours...
  100. pmc Structural localization of the E3 glycoprotein in attenuated Sindbis virus mutants
    A M Paredes
    National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry, Baylor College of Medicine, Houston, Texas 77030, USA
    J Virol 72:1534-41. 1998
    We have determined the three-dimensional structures of the wild-type Sindbis virus and two of its mutants that retain the E3 sequence within PE2...
  101. pmc The RNA interference pathway affects midgut infection- and escape barriers for Sindbis virus in Aedes aegypti
    Cynthia C H Khoo
    Arthropod Borne and Infectious Diseases Laboratory, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    BMC Microbiol 10:130. 2010
    ..b>Sindbis virus (SINV; family: Togaviridae, genus: Alphavirus) is an arbovirus that infects Ae. aegypti in the laboratory...

Research Grants63

  1. The Role of Interleukin-10 During Fatal Alphavirus Encephalomyelitis
    KIRSTEN KULCSAR; Fiscal Year: 2013
    ..b>Sindbis virus (SV), the prototypic alphavirus, is related to Venezuelan, eastern, and western equine encephalitis viruses, ..
  2. Control of alphavirous replacation in the nervous system
    Diane E Griffin; Fiscal Year: 2013
    ..Our studies of the prototype alphavirus, Sindbis virus (SINV), in mice have shown that neurons are the primary target cells and that both virus and host factors ..
  3. MOLECULAR BASIS OF ALPHAVIRUS ASSEMBLY
    Richard J Kuhn; Fiscal Year: 2013
    ..in the severity of disease induced in humans and other animals, from sub-clinical infections with Sindbis virus to fatal encephalitis that can be seen with eastern and Venezuelan equine encephalitis viruses...
  4. Mechanisms of apoptosis in the central nervous system
    Kenneth Tyler; Fiscal Year: 2009
    ..activation of these pathways in the brain following infection with other encephalitic viruses, including JEV, Sindbis virus (SINV), and Venezualen equine encephalitis virus (VEEV). OBJECTIVE 2...
  5. Efficacy of the antiviral state versus Sindbis and Venezuelan equine encephalitis
    William B Klimstra; Fiscal Year: 2013
    ..virus (VEEV), is much more resistant to the activity of the antiviral state than the human-avirulent Sindbis virus (SINV) and that the virulence of the viruses in animal models is largely reflective of these differences...
  6. Characterization of the La Crosse Virus glycoprotein fusion peptide
    SAMANTHA STANDISH SOLDAN; Fiscal Year: 2012
    ..similarities between LACV Gc amino acid region 970-1350 and the E1 fusion protein of two alphaviruses: Sindbis virus and Semliki Forrest virus (SFV)...
  7. MOLECULAR MECHANISMS OF ALPHAVIRUS ENTRY AND EXIT
    Margaret Kielian; Fiscal Year: 2013
    ..Our studies will focus on the highly-developed alphavirus experimental systems Semliki Forest virus and sindbis virus, and the emerging alphavirus pathogen chikungunya virus...
  8. MR and BL Tracking of Stem/Progenitor Cells in the CNS
    Jeff W Bulte; Fiscal Year: 2013
    ..e., a ricin-induced model as a localized, monophasic and a neurotropic Sindbis virus (NSV)-induced as a global, inflammatory disease model...
  9. Structural Studies of Alphavirus Membrane Fusion In Situ
    Wei Zhang; Fiscal Year: 2010
    ..RELEVANCE: A key step in the infection of enveloped viruses, such as influenza virus, West Nile virus and Sindbis virus, into the host cell, is fusion of the virus envelope with the host cell membrane...
  10. Protection of Mice From Lethal Alphavirus Encephalitis
    David N Irani; Fiscal Year: 2010
    ..S. Public Health Service given their potential use as bioterrorism agents. One alphavirus, Sindbis virus (SV), has been extensively studied in a murine model...
  11. Targeting host deubiquitinases for broad spectrum anti-infective therapy
    Christiane Wobus; Fiscal Year: 2013
    ..Salmonella enterica serovar Typhimurium);viruses (murine and human norovirus, encephalomyocarditis virus, Sindbis virus, La Crosse virus);and an apicomplexan parasite (Toxoplasma gondii)...
  12. Immune Evasion Mechanisms of Neurovirulent Alphaviruses
    Mark T Heise; Fiscal Year: 2012
    ..In studying the adult mouse neurovirulent AR86 strain of Sindbis virus;our laboratory has identified several determinants of adult mouse neurovirulence within the viral ..
  13. Novel host factors in selective autophagy of viruses
    Rhea M Sumpter; Fiscal Year: 2013
    ..genes, the E3 ubiquitin (Ub) ligase and p62- interacting protein, Smurf1, is a novel virophagy factor for Sindbis virus (SIN) and herpes simplex virus (HSV)-1...
  14. TRIM Interactions with Arthritic Alphaviruses
    Mark T Heise; Fiscal Year: 2013
    ..both TRIM21 and TRIM34 have antiviral activity against CHIKV and RRV, but not against two other alphaviruses, Sindbis virus and Venezuelan equine encephalitis virus (VEEV), suggesting that TRIM21 and TRIM34 specifically interact with ..
  15. Naturally occurring human antibodies to alphavirus infection
    Christopher F Basler; Fiscal Year: 2013
    ..Although past studies have characterized antibody responses to prototypical alphaviruses, such as Sindbis virus, in mice;little is known regarding the specific epitopes or the repertoire of human antibodies to natural ..
  16. Alphavirus Modulation of the Host Type I Interferon System
    Catherine Cruz; Fiscal Year: 2010
    ..However, little is known about these initial interactions. Evidence from our lab suggests that the Sindbis Virus (AR86) nonstructural proteins may antagonize type I IFN induction independently of the nonstructural proteins ..
  17. Regulation of Sindbis Virus Subgenomic RNA Synthesis
    Victor Stollar; Fiscal Year: 2010
    b>Sindbis virus, (SV) one of the simplest, enveloped RNA viruses, is the prototype virus of the family Togaviridae, genus alphavirus. It is transmitted to its vertebrate hosts by mosquitoes...
  18. Developing Transgenic Sindbis Virus as a Tool to Trace Neural Circuitry
    THOMAS ROBERT CLANDININ; Fiscal Year: 2013
    ..In addition, the development of a new tool for defining the wiring diagram of neural circuits will enable further studies of brain function and dysfunction. ..
  19. Dissecting the alphavirus entry receptor NRAMP
    Sara Cherry; Fiscal Year: 2013
    ..However, the viral receptors that mediate alphavirus entry are poorly understood. A genome wide screen using Sindbis virus identified NRAMP as a potential alphavirus entry receptor in insect cells...
  20. Upregulation of ZAP expression as a novel approach for anti-alphavirus therapy
    Margaret R Macdonald; Fiscal Year: 2013
    ..is a host protein that when over expressed mediates potent inhibition of alphaviruses, including the prototype Sindbis virus, as well as viruses in the Retroviridae and Filoviridae families...
  21. Mechanisms of biogenesis of atypical alphaviruses
    RAMASAMY RAJU; Fiscal Year: 2011
    ..b>Sindbis virus (SIN) is a human alphavirus and is one of the best studied model system for RNA genome structure, function and ..
  22. A Sindbis virus-based Vaccine Against RVFV Infection
    Ilya Frolov; Fiscal Year: 2006
    ..We propose to develop a prototype recombinant vaccine against RVFV infection based on Sindbis virus replicons expressing structural proteins of RVFV...
  23. Sindbis nonstructural protein nsP2: Interaction with cellular proteins
    Elena I Frolova; Fiscal Year: 2012
    ..b>Sindbis virus (SINV) is the most valuable tool for examining fundamental issues pertaining to alphavirus replication and ..
  24. Defining the determinants on the alphavirus receptor NRAMP required for virus bin
    Sara Cherry; Fiscal Year: 2013
    ..However, the viral receptors that mediate alphavirus entry are poorly understood. A genome wide screen using Sindbis virus identified NRAMP as a potential alphavirus entry receptor in insect cells...
  25. Replicon-based screening for inhibitors of alphaviruses
    PAUL OLIVO; Fiscal Year: 2005
    ..b>Sindbis virus and VEEV are prototypic alphaviruses that have been extensively used as vectors for expression of foreign ..
  26. Novel antiviral activities of tumor necrosis factor-alpha
    Lynn B Dustin; Fiscal Year: 2013
    ..b>Sindbis virus, an alphavirus, infects Huh-7 hepatoma cells and is insensitive to TNF1 pretreatment...
  27. A Viral Vector for Gene Expression in Insects
    Ranjit Dasgupta; Fiscal Year: 2007
    ..Vectors derived from Sindbis virus (SIN) have proven to be very useful in this type of research and few other viral vectors are under development...
  28. AMPA Receptor Trafficking and Signaling Complexes
    Edward B Ziff; Fiscal Year: 2010
    ..mutant GluR2 subunits and single pass chimeras bearing the GluR2 C terminus in hippocampal neurons from sindbis virus vectors and measure a series of phenotypes during synapse formation and modification...
  29. Alphavirus Replicons for Mucosal HIV Vaccines
    Michael Vajdy; Fiscal Year: 2002
    ..We recently discovered that our Sindbis virus-based non-replicating particles expressing HIV-1 gag (SIN-gag), which had been successfully used for ..
  30. RNA REPAIR AND RECOMBINATION--AN ALPHAVIRUS MODEL
    RAMASAMY RAJU; Fiscal Year: 2001
    ..b>Sindbis virus (SIN), the subject of this proposal, is a mosquito-transmitted human alphavirus...
  31. Retargeting of Retroviral Vectors
    Irvin S Y Chen; Fiscal Year: 2010
    ..significant progress in developing oncoretroviral and lentiviral vectors, in combination with pseudotypes of Sindbis virus envelope, for stable transduction of genes delivered via the bloodstream...
  32. Development of a genetically tractable nematode model of alphavirus infection
    Margaret R Macdonald; Fiscal Year: 2010
    ..Techniques for mutagenesis and genetic analysis of C. elegans are well worked out. The type Alphavirus, Sindbis virus (SINV), was chosen as a test Alphavirus for model development...
  33. Nucleotide Pools and the Replication of Sindbis Virus
    Victor Stollar; Fiscal Year: 2003
    ..b>Sindbis virus (SV), a mosquito-transmitted arbovirus, and the prototype virus of the family Togaviridae, genus aiphavirus, ..
  34. GENETIC ANALYSIS OF SINDBIS VIRUS
    James Strauss; Fiscal Year: 2000
    ..The applicants also propose to examine the contributions of the virus structural proteins to RNA replication. ..
  35. Characterizing the Role of Encapsidated Host Ribosomes in Alphavirus Infectivity
    KEVIN JOSEPH SOKOLOSKI; Fiscal Year: 2013
    ..Examination of mosquito derived Sindbis virus (SINV) has indicated that host ribosomal components are incorporated into the viral particles...
  36. MOLECULAR GENETICS OF SINDBIS VIRUS REPLICATION
    Charles Rice; Fiscal Year: 2005
    ..The experiments detailed in this proposal concern our current studies on Sindbis virus (SIN), type virus of the family Togaviridae...
  37. GTP-BINDING PROTEIN RHO IN T CELL SIGNALING PATHWAYS
    Jonathan Moorman; Fiscal Year: 2000
    ..Using a transient expression system based on Sindbis virus we will express Rho proteins, including constitutively active and dominant negative mutants...
  38. BCL2 AND BECLIN AND ALPHAVIRUS PATHOGENESIS
    Beth Levine; Fiscal Year: 2003
    ..Beclin, in the pathogenesis of Sindbis virus encephalitis...
  39. Interaction of Sindbis Virus with Cellular Processes
    Ilya Frolov; Fiscal Year: 2002
    ..b>Sindbis virus is one of the least pathogenic among the alphaviruses...
  40. ARBOVIRUS REPLICATION IN MOSQUITO AND VERTEBRATE CELLS
    Victor Stollar; Fiscal Year: 1991
    ..b>Sindbis virus (SV) in the family Togaviridae, genus alphavirus, is the subject of this proposal...
  41. NOVEL STRATEGIES TO LIMIT NEURONAL APOPTOSIS
    Rajiv Ratan; Fiscal Year: 2001
    ..agent, we examined the effects of this antioxidant in another paradigm of neuronal apoptosis: infection with Sindbis Virus (SV)...
  42. Biology-Pathology of Arbovirus Persistence in a Mosquito Host
    Doria Bowers; Fiscal Year: 2007
    ..will be examined following oral inoculation of adult female mosquitoes with the prototype Alphavirus, Sindbis virus (SIN). Specific Aims include I: Selection for a colony of Ae...
  43. Function and Mechanisms of the N-End Rule Pathway
    Yong Tae Kwon; Fiscal Year: 2007
    ..amphibian limb regeneration, apoptosis, muscle wasting, and the degradation of various proteins (Sindbis virus RNA polymerase, HIV integrase, the Listeria monocytogenes p60, RGS4 and RGS16, and the encephalomyocarditis ..
  44. GENOME OF THE CELL FUSING AGENT
    Victor Stollar; Fiscal Year: 1991
    ..Mutants of CFA will be selected, as we have done with Sindbis virus, which code for altered RNA capping and methylating enzymes...
  45. STRUCTURE AND ASSEMBLY OF AN ALPHAVIRUS
    Dennis Brown; Fiscal Year: 2003
    ..b>Sindbis virus is the prototype of the alphavirus group and it has been shown that Aedes albopictus is an efficient vector of ..
  46. TRANSCRIPTION FACTOR NF-E2 IN ALPHA IIB BETA 3 SIGNALING
    SANFORD SHATTIL; Fiscal Year: 2004
    ..Recombinant p45 will be expressed using retroviral and Sindbis virus systems...
  47. Targeted in vivo gene transfer to hematopoietic stem cells
    Nikunj Somia; Fiscal Year: 2009
    ..surface receptors expressed on HCS (including CD34, and the kinase receptors c-kit and Flt3) onto a mutant Sindbis virus envelope that is severely impaired for binding its receptor but is competent for membrane fusion...
  48. Role of SMN in Neuronal Apoptosis
    J Hardwick; Fiscal Year: 2006
    ..We have developed the Sindbis virus vector system for assessing the functions of apoptosis regulators in cultured neurons and in the central ..
  49. MECHANISMS OF PATHOLOGIC NEURONAL APOPTOSIS
    Rajiv Ratan; Fiscal Year: 2001
    ..agent, we examined the effects of this antioxidant in another paradigm of neuronal apoptosis: infection with Sindbis Virus (SV)...
  50. VIRUS-INDUCED AUTOIMMUNE MEDIATED ENCEPHALOMYELITIS
    Foroozan Mokhtarian; Fiscal Year: 1991
    ..role of immune and infammatory responses to two acute viral infections of mice, Semliki Forest Virus and Sindbis Virus to induce, or increase, susceptibility to the induction of a disease process similar to experimental allergic ..
  51. MOLECULAR DETERMINANTS OF ALPHAVIRUS INDUCED APOPTOSIS
    Beth Levine; Fiscal Year: 2000
    ..Here, she proposes to use Sindbis virus (SIN), the prototype alphavirus, to investigate virally-induced apoptosis in several mammalian cell lines and ..
  52. The Intrinsic Neuronal Growth State and CNS Regeneration
    Carole Ho; Fiscal Year: 2006
    ..significance of the transcription factors identified will be tested in a primary neuronal culture assay using Sindbis virus-mediated over-expression analysis and Lentivirus-mediated delivery of interfering RNA (RNAi) knockdown...
  53. Effect of ethanol on dopamine receptor trafficking
    LAUREA DIAZ; Fiscal Year: 2004
    Dopamine receptors (D1) will be tagged with green fluorescent protein (GFP), and inserted into the sindbis virus. This virus will be injected into organotypic cultures that include the mesolimbic pathway, the projections of the ventral ..
  54. Transgenic indicator cells for influenza virus
    PAUL OLIVO; Fiscal Year: 2006
    ..Previously, we developed and patented methods for detecting and quantitating other RNA viruses such as Sindbis virus, a positive-strand RNA virus and respiratory syncytial virus (RSV), a negative-strand RNA virus...
  55. REGULATION OF VIRAL INFECTIONS BY CELLULAR GENES
    J Marie Hardwick; Fiscal Year: 2009
    b>Sindbis virus is an alphavirus that is transmitted by mosquitoes to animal and human hosts in many geographical locations worldwide...
  56. Optimizing Foamy Virus Vectors for Gene Therapy & Vaccine Applications
    WILLAM HARDY; Fiscal Year: 2006
    ..Lentiviral-based, recombinant Sindbis virus pseudovirions displaying the IgG-binding domain of protein A (Z domain) in the E2 region of the viral envelope ..
  57. Glial Cells in the Immune Response to Alphavirus
    Jessica Darman; Fiscal Year: 2005
    b>Sindbis Virus (SV) is an alphavirus that induces an immune response in the central nervous system (CNS) of infected mice...
  58. Targetable Vectors for Gene Therapy
    Daniel Meruelo; Fiscal Year: 2004
    ..The approach is based on the observation that mammalian cells are fully susceptible to Sindbis virus mediated apoptosis, while insect cells are completely resistant...
  59. STRUCTURE AND FUNCTION OF VIRAL GLYCOPEPTIDES
    Lawrence Hunt; Fiscal Year: 1980
    ..The advantages of studying cells in culture that are infected with vesicular stomatitis virus (VSV) or Sindbis virus are three-fold: (1) the genetic information of these viruses is limited and specifies only one (VSV) or two (..
  60. ARBOVIRUS GROWTH IN CULTURED INSECT CELLS
    Dennis Brown; Fiscal Year: 1992
    b>Sindbis virus will be employed as a model insect-vectored virus in studies investigating the interaction of these agents with the invertebrate hosts...
  61. Autophagy: A Novel Antiviral Host Defense Pathway
    Beth Levine; Fiscal Year: 2007
    ..hypothesis, we have previously shown that the first identified mammalian autophagy gene, beclin 1, inhibits Sindbis virus replication and protects mice against lethal Sindbis virus encephalitis...
  62. Autophagy:A Novel Antiviral Host Defense Pathway
    Beth Levine; Fiscal Year: 2002
    ..hypothesis, we have previously shown that the first identified mammalian autophagy gene, beclin 1, inhibits Sindbis virus replication and protects mice against lethal Sindbis virus encephalitis...
  63. RIBOZYMES-- CATALYSIS AND ANTIVIRAL ACTIVITY
    John Burke; Fiscal Year: 2004
    ..we have focused on studies conducted entirely within the PI's lab in which we have demonstrated inhibition of Sindbis virus in BHK-21 cells stably expressing hairpin ribozymes...