flavivirus

Summary

Summary: A genus of FLAVIVIRIDAE containing several subgroups and many species. Most are arboviruses transmitted by mosquitoes or ticks. The type species is YELLOW FEVER VIRUS.

Top Publications

  1. ncbi A structural perspective of the flavivirus life cycle
    Suchetana Mukhopadhyay
    Department of Biological Sciences, Purdue University, 915 West State Street, West Lafayette, Indiana 47907 2054, USA
    Nat Rev Microbiol 3:13-22. 2005
  2. doi Pathogenic flaviviruses
    E A Gould
    CEH Oxford, Mansfield Road, Oxford, UK
    Lancet 371:500-9. 2008
  3. pmc Evidence of simultaneous circulation of West Nile and Usutu viruses in mosquitoes sampled in Emilia-Romagna region (Italy) in 2009
    Mattia Calzolari
    Istituto Zooprofilattico Sperimentale della Lombardia e dell Emilia Romagna B Ubertini IZSLER, Brescia, Italy
    PLoS ONE 5:e14324. 2010
  4. pmc Phylogeny of the genus Flavivirus
    G Kuno
    Division of Vector Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado 80522 2087, USA
    J Virol 72:73-83. 1998
  5. doi Molecular mechanisms of flavivirus membrane fusion
    Karin Stiasny
    Institute of Virology, Medical University of Vienna, AT, Austria
    Amino Acids 41:1159-63. 2011
  6. doi Arboviral survey of mosquitoes in two northern Italian regions in 2007 and 2008
    Mattia Calzolari
    Istituto Zooprofilattico Sperimentale della Lombardia e dell Emilia Romagna B Ubertini, Brescia, Italy
    Vector Borne Zoonotic Dis 10:875-84. 2010
  7. doi Isolation and characterization of a new insect flavivirus from Aedes albopictus and Aedes flavopictus mosquitoes in Japan
    Keita Hoshino
    Department of Medical Entomology, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku, Tokyo 162 8640, Japan
    Virology 391:119-29. 2009
  8. pmc Binding of flavivirus nonstructural protein NS1 to C4b binding protein modulates complement activation
    Panisadee Avirutnan
    Department of Medicine, Washington University School of Medicine, St Louis, MO 63110, USA
    J Immunol 187:424-33. 2011
  9. pmc Molecular mechanisms involved in the early steps of flavivirus cell entry
    Bärbel Kaufmann
    Department of Biological Sciences, Purdue University, 915 West State Street, West Lafayette, IN 47907 2054, USA
    Microbes Infect 13:1-9. 2011
  10. ncbi Subcellular localization and some biochemical properties of the flavivirus Kunjin nonstructural proteins NS2A and NS4A
    J M MacKenzie
    Sir Albert Sakzewski Virus Research Centre, Royal Children s Hospital, Herston, Brisbane, Australia
    Virology 245:203-15. 1998

Detail Information

Publications305 found, 100 shown here

  1. ncbi A structural perspective of the flavivirus life cycle
    Suchetana Mukhopadhyay
    Department of Biological Sciences, Purdue University, 915 West State Street, West Lafayette, Indiana 47907 2054, USA
    Nat Rev Microbiol 3:13-22. 2005
    Dengue, Japanese encephalitis, West Nile and yellow fever belong to the Flavivirus genus, which is a member of the Flaviviridae family...
  2. doi Pathogenic flaviviruses
    E A Gould
    CEH Oxford, Mansfield Road, Oxford, UK
    Lancet 371:500-9. 2008
    ....
  3. pmc Evidence of simultaneous circulation of West Nile and Usutu viruses in mosquitoes sampled in Emilia-Romagna region (Italy) in 2009
    Mattia Calzolari
    Istituto Zooprofilattico Sperimentale della Lombardia e dell Emilia Romagna B Ubertini IZSLER, Brescia, Italy
    PLoS ONE 5:e14324. 2010
    ..An extensive entomological survey was performed in 2009 to establish the presence and distribution of mosquito arboviruses in this region, with particular reference to flaviviruses...
  4. pmc Phylogeny of the genus Flavivirus
    G Kuno
    Division of Vector Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado 80522 2087, USA
    J Virol 72:73-83. 1998
    ..a comprehensive phylogenetic study to establish the genetic relationship among the viruses of the genus Flavivirus and to compare the classification based on molecular phylogeny with the existing serologic method...
  5. doi Molecular mechanisms of flavivirus membrane fusion
    Karin Stiasny
    Institute of Virology, Medical University of Vienna, AT, Austria
    Amino Acids 41:1159-63. 2011
    ..encephalitis virus as a model, we have defined individual steps of the molecular processes underlying the flavivirus fusion mechanisms...
  6. doi Arboviral survey of mosquitoes in two northern Italian regions in 2007 and 2008
    Mattia Calzolari
    Istituto Zooprofilattico Sperimentale della Lombardia e dell Emilia Romagna B Ubertini, Brescia, Italy
    Vector Borne Zoonotic Dis 10:875-84. 2010
    ..caspius mosquitoes and Batai virus in a pool of Anopheles maculipennis mosquitoes, and (3) the presence of flavivirus RNAs in pools of Oc...
  7. doi Isolation and characterization of a new insect flavivirus from Aedes albopictus and Aedes flavopictus mosquitoes in Japan
    Keita Hoshino
    Department of Medical Entomology, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku, Tokyo 162 8640, Japan
    Virology 391:119-29. 2009
    We isolated a new flavivirus from Aedes albopictus mosquito and a related species in Japan. The virus, designated Aedes flavivirus (AEFV), only replicated in a mosquito cell line and produced a mild cytopathic effect...
  8. pmc Binding of flavivirus nonstructural protein NS1 to C4b binding protein modulates complement activation
    Panisadee Avirutnan
    Department of Medicine, Washington University School of Medicine, St Louis, MO 63110, USA
    J Immunol 187:424-33. 2011
    ..b>Flavivirus nonstructural protein 1 (NS1) is a secreted nonstructural glycoprotein that accumulates in plasma to high levels ..
  9. pmc Molecular mechanisms involved in the early steps of flavivirus cell entry
    Bärbel Kaufmann
    Department of Biological Sciences, Purdue University, 915 West State Street, West Lafayette, IN 47907 2054, USA
    Microbes Infect 13:1-9. 2011
    ..Recent findings on these early steps in the life cycle of flaviviruses are the focus of this review...
  10. ncbi Subcellular localization and some biochemical properties of the flavivirus Kunjin nonstructural proteins NS2A and NS4A
    J M MacKenzie
    Sir Albert Sakzewski Virus Research Centre, Royal Children s Hospital, Herston, Brisbane, Australia
    Virology 245:203-15. 1998
    ..Taken together the results indicate that the flavivirus replication complex includes NS2A and NS4A in the VP in addition to the previously identified NS1 and NS3.
  11. ncbi Sequences of flavivirus-related RNA viruses persist in DNA form integrated in the genome of Aedes spp. mosquitoes
    Sandrine Crochu
    Unite des Virus Emergents, Faculte de Medecine de Marseille, IFR48 IRD UR034, 27 Boulevard Jean Moulin, 13005 Marseille, France
    J Gen Virol 85:1971-80. 2004
    b>Flavivirus-related sequences have been discovered in the dsDNA genome of Aedes albopictus and Aedes aegypti mosquitoes, demonstrating for the first time an integration into a eukaryotic genome of a multigenic sequence from an RNA virus ..
  12. ncbi Origins, evolution, and vector/host coadaptations within the genus Flavivirus
    Ernest A Gould
    Institute of Virology and Environmental Microbiology, Oxford University, Oxford, OX1 3SR, United Kingdom
    Adv Virus Res 59:277-314. 2003
    Although viruses in the genus Flavivirus share complex antigenic interrelationships, they can be divided into four phylogenetic/ecological groups: two mosquito-borne groups, a tick-borne group, and nonvectored viruses...
  13. ncbi Flavivirus susceptibility in Aedes aegypti
    William C Black
    Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    Arch Med Res 33:379-88. 2002
    ..and present research on genetic components and environmental factors in Aedes aegypti that appear to control flavivirus transmission. We review genetic relationships among Ae...
  14. doi Pathogenesis of flavivirus infections: using and abusing the host cell
    Maria Dolores Fernandez-Garcia
    Unité Pathogénie Virale, INSERM U819, Institut Pasteur, Departement de Virologie, Paris, France
    Cell Host Microbe 5:318-28. 2009
    ..Considerable progress has been made in understanding flavivirus structure and replication strategies, but only now are the complex molecular interactions between the virus and ..
  15. doi Role of host cell factors in flavivirus infection: Implications for pathogenesis and development of antiviral drugs
    Boris Pastorino
    Unite des Virus Emergents, UMR190 Emergence des pathologies virales Université de la Méditerranée, Institut de Recherche pour le Developpement, Faculte de Medecine, Marseille, France
    Antiviral Res 87:281-94. 2010
    The genus Flavivirus contains approximately 70 arthropod-borne enveloped RNA viruses many of which cause severe human and in some cases, animal disease...
  16. ncbi Flavivirus induces interferon-beta gene expression through a pathway involving RIG-I-dependent IRF-3 and PI3K-dependent NF-kappaB activation
    Tsung Hsien Chang
    Graduate Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan, ROC
    Microbes Infect 8:157-71. 2006
    ..acid-inducible gene I (RIG-I), and a cellular kinase, phosphatidylinositol-3 kinase (PI3K), are required for flavivirus-induced IRF-3 and NF-kappaB activation, respectively...
  17. doi Genome cyclization as strategy for flavivirus RNA replication
    Sergio M Villordo
    Fundacion Instituto Leloir, Patricias Argentinas 435, Buenos Aires, Argentina
    Virus Res 139:230-9. 2009
    ..The circular conformation of flavivirus genomes was demonstrated to be essential for RNA amplification...
  18. pmc The flavivirus NS5 protein is a true RNA guanylyltransferase that catalyzes a two-step reaction to form the RNA cap structure
    Moheshwarnath Issur
    Departement de Biochimie, Faculte de Medecine, Universite de Sherbrooke, Sherbrooke, Quebec J1H 5N4, Canada
    RNA 15:2340-50. 2009
    The 5'-end of the flavivirus genome harbors a methylated (m7)GpppA(2'OMe) cap structure, which is generated by the virus-encoded RNA triphosphatase, RNA (guanine-N7) methyltransferase, nucleoside 2'-O-methyltransferase, and RNA ..
  19. pmc Molecular evolution of the insect-specific flaviviruses
    Shelley Cook
    Natural History Museum, Cromwell Road, London SW7 5BD, UK
    J Gen Virol 93:223-34. 2012
    ..Herein we review all 'insect-specific' flavivirus sequences currently available and conduct phylogenetic analyses of both the 'insect-specific' flaviviruses and ..
  20. ncbi Flavivirus encephalitis
    Tom Solomon
    Department of Neurological Science, University of Liverpool, Liverpool, United Kingdom
    N Engl J Med 351:370-8. 2004
  21. pmc Comparison of flavivirus universal primer pairs and development of a rapid, highly sensitive heminested reverse transcription-PCR assay for detection of flaviviruses targeted to a conserved region of the NS5 gene sequences
    N Scaramozzino
    , , Grenoble, France
    J Clin Microbiol 39:1922-7. 2001
    ..The six published Flavivirus genus primer pairs localized in the NS1, NS3, NS5, and 3' NC regions were evaluated in terms of specificity and ..
  22. pmc Molecular targets for flavivirus drug discovery
    Aruna Sampath
    National Center for Natural Product Research, Research Institute of Pharmaceutical Sciences, School of Pharmacy, University of Mississippi, University, MS 38677, USA
    Antiviral Res 81:6-15. 2009
    ..Antiviral therapy could potentially reduce morbidity and mortality from flavivirus infections, but no effective drugs are currently available...
  23. doi The flavivirus NS1 protein: molecular and structural biology, immunology, role in pathogenesis and application as a diagnostic biomarker
    David A Muller
    School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, Queensland 4072, Australia
    Antiviral Res 98:192-208. 2013
    The flavivirus nonstructural glycoprotein NS1 is an enigmatic protein whose structure and mechanistic function have remained somewhat elusive ever since it was first reported in 1970 as a viral antigen circulating in the sera of dengue-..
  24. pmc A new insect-specific flavivirus from northern Australia suppresses replication of West Nile virus and Murray Valley encephalitis virus in co-infected mosquito cells
    Jody Hobson-Peters
    Australian Infectious Diseases Research Centre, School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, Queensland, Australia
    PLoS ONE 8:e56534. 2013
    ..assess whether such viruses occur locally, we used reverse transcription-polymerase chain reaction (RT-PCR) and flavivirus universal primers that are specific to the NS5 gene to detect these viruses in mosquito pools collected from the ..
  25. pmc RNA structures required for production of subgenomic flavivirus RNA
    Anneke Funk
    Centre for Infectious Disease Research, School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, QLD 4072, Australia
    J Virol 84:11407-17. 2010
    ..5 kb) derived from the 3' untranslated region (UTR) of the genomic RNA (gRNA), which is required for flavivirus-induced cytopathicity and pathogenicity (G. P. Pijlman et al., Cell Host Microbe, 4: 579-591, 2008)...
  26. pmc Structures of immature flavivirus particles
    Ying Zhang
    Department of Biological Sciences, Lilly Hall, 915 West State Street, Purdue University, West Lafayette, IN 47907 2054, USA
    EMBO J 22:2604-13. 2003
    ....
  27. pmc Insect-specific flaviviruses from Culex mosquitoes in Colorado, with evidence of vertical transmission
    Bethany G Bolling
    Arthropod Borne and Infectious Diseases Laboratory, Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, Colorado 80521, USA
    Am J Trop Med Hyg 85:169-77. 2011
    ..We used universal flavivirus primers to test pools of Culex mosquitoes for viral RNA...
  28. ncbi Complete genome analysis and molecular characterization of Usutu virus that emerged in Austria in 2001: comparison with the South African strain SAAR-1776 and other flaviviruses
    Tamas Bakonyi
    Zoonoses and Emerging Infections Group, Clinical Virology, Clinical Department of Diagnostics, University of Veterinary Medicine, Vienna, A 1210 Vienna, Austria
    Virology 328:301-10. 2004
    ..the complete genome sequences of two strains of Usutu virus (USUV), a mosquito-borne member of the genus Flavivirus in the Japanese encephalitis virus (JEV) serogroup...
  29. pmc Structural insights into the mechanisms of antibody-mediated neutralization of flavivirus infection: implications for vaccine development
    Theodore C Pierson
    Viral Pathogenesis Section, Laboratory of Viral Diseases, National Institutes of Health, 33 North Drive, Building 33, Room 1E19A 2, Bethesda, MD 20892, USA
    Cell Host Microbe 4:229-38. 2008
    ..is to elicit a protective humoral response directed against the envelope proteins arrayed on the surface of the flavivirus virion...
  30. doi Detection of mosquito-only flaviviruses in Europe
    Mattia Calzolari
    Istituto Zooprofilattico Sperimentale della Lombardia e dell Emilia Romagna B Ubertini IZSLER, Via Bianchi 9, 25124, Brescia, Italy
    J Gen Virol 93:1215-25. 2012
    The genus Flavivirus, family Flaviviridae, includes a number of important arthropod-transmitted human pathogens such as dengue viruses, West Nile virus, Japanese encephalitis virus and yellow fever virus...
  31. pmc Structure and function of flavivirus NS5 methyltransferase
    Yangsheng Zhou
    Wadsworth Center, New York State Department of Health, 120 New Scotland Ave, Albany, NY 12208, USA
    J Virol 81:3891-903. 2007
    The plus-strand RNA genome of flavivirus contains a 5' terminal cap 1 structure (m7GpppAmG)...
  32. ncbi Antigenic structure of flavivirus proteins
    John T Roehrig
    Division of Vector Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, U S Department of Health and Human Services, Fort Collins, Colorado 80521, USA
    Adv Virus Res 59:141-75. 2003
    ..These facts warrant continued efforts to decode all facets of flavivirus immunology...
  33. pmc Flavivirus activates phosphatidylinositol 3-kinase signaling to block caspase-dependent apoptotic cell death at the early stage of virus infection
    Chyan Jang Lee
    Graduate Institute of Life Sciences, Taipei, Taiwan, Republic of China
    J Virol 79:8388-99. 2005
    ..cells at a rather late stage of infection, we evaluated the possible roles of the PI3K/Akt signaling pathway in flavivirus-infected cells...
  34. ncbi Tick-borne flaviviruses
    T S Gritsun
    CEH Institute of Virology and Environmental Microbiology, Oxford, OX1 3SR, United Kingdom
    Adv Virus Res 61:317-71. 2003
    ..The other viruses in the tick-borne flavivirus group are also included despite the fact that they do not play an essential epidemiologic role in humans...
  35. ncbi A real-time RT-PCR method for the universal detection and identification of flaviviruses
    G Moureau
    Unite des Virus Emergents, Faculte de Medecine, Marseille, France
    Vector Borne Zoonotic Dis 7:467-77. 2007
    ..It combines the convenient real-time polymerase chain reaction (PCR) format with a broad spectrum of flavivirus detection...
  36. pmc Emergence of Usutu virus in Hungary
    Tamas Bakonyi
    Department of Microbiology and Infectious Diseases, Faculty of Veterinary Science, Szent Istvan University, Hungária krt 23 25, H 1143, Budapest, Hungary
    J Clin Microbiol 45:3870-4. 2007
    In 2001, Usutu virus (USUV), a mosquito-borne flavivirus of the Japanese encephalitis virus serogroup related to West Nile virus and previously restricted to sub-Saharan Africa, emerged in wild and zoo birds in and around Vienna, Austria...
  37. pmc Molecular mechanisms of antibody-mediated neutralisation of flavivirus infection
    Theodore C Pierson
    Viral Pathogenesis Section, Laboratory of Viral Diseases, NIAID, NIH, Bethesda, MD 20892, USA
    Expert Rev Mol Med 10:e12. 2008
    ..in our understanding of the structural biology of flaviviruses, coupled with the use of small-animal models of flavivirus infection, have promoted significant advances in our appreciation of the factors that govern antibody ..
  38. ncbi Emerging flaviviruses: the spread and resurgence of Japanese encephalitis, West Nile and dengue viruses
    John S Mackenzie
    Australian Biosecurity Cooperative Research Centre, Curtin University of Technology, Perth, Western Australia
    Nat Med 10:S98-109. 2004
    ....
  39. ncbi Isolation of a new strain of the flavivirus cell fusing agent virus in a natural mosquito population from Puerto Rico
    Shelley Cook
    Department of Zoology, University of Oxford, South Parks Road, Oxford OX1 3PS, UK
    J Gen Virol 87:735-48. 2006
    The genus Flavivirus contains approximately 70 single-stranded, positive-sense RNA viruses that are mosquito-borne, tick-borne or have no known vector...
  40. pmc Evidence for ribosomal frameshifting and a novel overlapping gene in the genomes of insect-specific flaviviruses
    Andrew E Firth
    Biosciences Institute, University College Cork, Cork, Ireland
    Virology 399:153-66. 2010
    ..Cell fusing agent virus, Kamiti River virus, Culex flavivirus and several recently discovered flaviviruses have no known vertebrate host and apparently infect only insects...
  41. doi The zoonotic flaviviruses of southern, south-eastern and eastern Asia, and Australasia: the potential for emergent viruses
    J S Mackenzie
    Australian Biosecurity Cooperative Research Centre for Emerging Infectious Diseases, Division of Health Sciences, Curtin University of Technology, Perth, Australia
    Zoonoses Public Health 56:338-56. 2009
    ..Furthermore, because of their ecology and dependence on climate, there is a strong likelihood that global warming may significantly increase the potential for disease emergence and/or spread...
  42. pmc Cholesterol effectively blocks entry of flavivirus
    Chyan Jang Lee
    Institute of Biomedical Sciences, Academia Sinica, Academy Rd, Nankang, Taipei 11529, Taiwan, Republic of China
    J Virol 82:6470-80. 2008
    ..Cholesterol mainly affected the early step of the flavivirus life cycle, because the presence of cholesterol during viral adsorption greatly blocked JEV and DEN-2 ..
  43. pmc Cyclosporine inhibits flavivirus replication through blocking the interaction between host cyclophilins and viral NS5 protein
    Min Qing
    State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China
    Antimicrob Agents Chemother 53:3226-35. 2009
    ..we report that cyclophilins (CyPs), a family of cellular peptidyl-prolyl isomerases (PPIases), play a role in flavivirus replication. Huh-7...
  44. pmc Essential role of cyclization sequences in flavivirus RNA replication
    A A Khromykh
    Sir Albert Sakzewski Virus Research Centre, Royal Children s Hospital, University of Queensland, Herston Rd, Herston, Brisbane, QLD 4029, Australia
    J Virol 75:6719-28. 2001
    ..for interactions between conserved complementary (cyclization) sequences in the 5'- and 3'-terminal regions of Flavivirus RNA was previously suggested but never tested in vivo...
  45. pmc Genetic characterization of Zika virus strains: geographic expansion of the Asian lineage
    Andrew D Haddow
    Institute for Human Infections and Immunity, Center for Tropical Diseases, Department of Pathology, University of Texas Medical Branch, Galveston, Texas, USA
    PLoS Negl Trop Dis 6:e1477. 2012
    Zika virus (ZIKV) is a mosquito-borne flavivirus distributed throughout much of Africa and Asia. Infection with the virus may cause acute febrile illness that clinically resembles dengue fever...
  46. ncbi Ultrastructural evidence of the ehrlichial developmental cycle in naturally infected Ixodes persulcatus ticks in the course of coinfection with Rickettsia, Borrelia, and a flavivirus
    Vsevolod L Popov
    Department of Pathology, The University of Texas Medical Branch, Center for Biodefense and Emerging Infectious Diseases, Galveston, Texas 77555, USA
    Vector Borne Zoonotic Dis 7:699-716. 2007
    ..coinfection with Borrelia burgdorferi sensu lato (sl), "Candidatus Rickettsia tarasevichiae," and a flavivirus (presumably, tick-borne encephalitis virus [TBEV]) of Ixodes persulcatusticks collected in the Cis-Ural region ..
  47. ncbi Genetic characterization of tick-borne flaviviruses: new insights into evolution, pathogenetic determinants and taxonomy
    Gilda Grard
    Unité des Virus Emergents EA3292, IFR48, IRD UR0178, Faculté de médecine La Timone, 27 Boulevard Jean Moulin, 13005 Marseille, France
    Virology 361:80-92. 2007
    Here, we analyze the complete coding sequences of all recognized tick-borne flavivirus species, including Gadgets Gully, Royal Farm and Karshi virus, seabird-associated flaviviruses, Kadam virus and previously uncharacterized isolates of ..
  48. ncbi Biological, antigenic and phylogenetic characterization of the flavivirus Alfuy
    Fiona J May
    Department of Microbiology and Parasitology, School of Molecular and Microbial Sciences, The University of Queensland, Building 76, Cooper Road, St Lucia, QLD 4072, Australia
    J Gen Virol 87:329-37. 2006
    Alfuy virus (ALFV) is classified as a subtype of the flavivirus Murray Valley encephalitis virus (MVEV); however, despite preliminary reports of antigenic and ecological similarities with MVEV, ALFV has not been associated with human ..
  49. doi Zika virus outbreak on Yap Island, Federated States of Micronesia
    Mark R Duffy
    Division of Vector Borne Infectious Diseases, National Center for Zoonotic, Vector Borne, and Enteric Diseases, Centers for Disease Control and Prevention, Fort Collins, CO 80521, USA
    N Engl J Med 360:2536-43. 2009
    ..No previous outbreaks and only 14 cases of Zika virus disease have been previously documented...
  50. ncbi Rapid subgroup identification of the flaviviruses using degenerate primer E-gene RT-PCR and site specific restriction enzyme analysis
    M W Gaunt
    Pathogen Molecular Biology and Biochemistry Unit, Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK
    J Virol Methods 128:113-27. 2005
    ..Categorisation of the flavivirus cDNA products into the corresponding virus groups was undertaken through restriction enzyme analysis by defining ..
  51. pmc Novel flaviviruses detected in different species of mosquitoes in Spain
    Ana Vazquez
    Laboratory of Arboviruses and Imported Viral Diseases, National Center for Microbiology, Instituto de Salud Carlos III ISCIII, Madrid, Spain
    Vector Borne Zoonotic Dis 12:223-9. 2012
    ..virus, a novel mosquito borne virus, was isolated from Ochlerotatus caspius mosquitoes; Spanish Ochlerotatus flavivirus and Spanish Culex flavivirus, two novel insect flaviviruses, were isolated from Oc...
  52. pmc PCP consensus sequences of flaviviruses: correlating variance with vector competence and disease phenotype
    Petr Danecek
    Sealy Center for Structural Biology and Molecular Biophysics, Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, 301 University Boulevard, Galveston, TX 77555 0857, USA
    J Mol Biol 396:550-63. 2010
    ..Computational methods are needed to design multivalent vaccines against flaviviruses (FVs) such as the West Nile virus or the dengue virus (DENV)...
  53. pmc Constancy and diversity in the flavivirus fusion peptide
    Stephen J Seligman
    Department of Microbiology and Immunology, New York Medical College, Valhalla, New York, USA
    Virol J 5:27. 2008
    ..The cell-fusing related agents, growing only in mosquitoes or insect cell lines, possess a different homologous peptide...
  54. pmc Detection of flaviviruses and orthobunyaviruses in mosquitoes in the Yucatan Peninsula of Mexico in 2008
    Jose A Farfan-Ale
    Laboratorio de Arbovirologia, Centro de Investigaciones Regionales Dr Hideyo Noguchi, Universidad Autonoma de Yucatan, Merida, Mexico
    Vector Borne Zoonotic Dis 10:777-83. 2010
    ..A subset (n=20,124) of Culex quinquefasciatus collected throughout the year was further tested by RT-PCR using flavivirus-specific primers. Flavivirus RNA was present in this mosquito species year-round...
  55. pmc Functional analysis of two cavities in flavivirus NS5 polymerase
    Gang Zou
    Novartis Institute for Tropical Diseases, Singapore 138670
    J Biol Chem 286:14362-72. 2011
    b>Flavivirus NS5 protein encodes methyltransferase and RNA-dependent RNA polymerase (RdRp) activities. Structural analysis of flavivirus RdRp domains uncovered two conserved cavities (A and B)...
  56. pmc Bagaza virus in partridges and pheasants, Spain, 2010
    Montserrat Agüero
    Laboratorio Central de Veterinaria, Algete, Spain
    Emerg Infect Dis 17:1498-501. 2011
    ..Reverse transcription PCR and virus isolation detected flavivirus infections...
  57. ncbi Safety testing for neurovirulence of novel live, attenuated flavivirus vaccines: infant mice provide an accurate surrogate for the test in monkeys
    Thomas P Monath
    Acambis Inc, 38 Sidney Street, Cambridge, MA 02139, USA
    Biologicals 33:131-44. 2005
    ..We have used yellow fever 17D virus as a live vector for novel flavivirus vaccines (designated ChimeriVax) against dengue, Japanese encephalitis (JE), and West Nile (WN) viruses...
  58. doi Isolation and characterization of a new mosquito flavivirus, Quang Binh virus, from Vietnam
    Mary B Crabtree
    Division of Vector Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, US Department of Health and Human Services, 3150 Rampart Road, Fort Collins, CO 80521, USA
    Arch Virol 154:857-60. 2009
    ..We describe here the isolation and characterization of a new mosquito-only flavivirus in this group...
  59. doi Assessment of a novel real-time pan-flavivirus RT-polymerase chain reaction
    Nicholas Johnson
    Rabies and Wildlife Zoonoses Group, Veterinary Laboratories Agency, Addlestone, United Kingdom
    Vector Borne Zoonotic Dis 10:665-71. 2010
    ..such as tick-borne encephalitis virus, dengue virus, and Usutu virus has prompted us to design a novel pan-flavivirus RT-polymerase chain reaction for the purpose of surveillance for a range of flaviviruses...
  60. pmc Distribution and phylogenetic comparisons of a novel mosquito flavivirus sequence present in Culex tarsalis Mosquitoes from western Canada with viruses isolated in California and Colorado
    Shaun Tyler
    National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada
    Am J Trop Med Hyg 85:162-8. 2011
    In a previous study, a new flavivirus genome sequence was identified in Culex tarsalis mosquitoes obtained in Alberta, Canada and was shown to be genetically related to but distinct from members of the insect-specific flaviviruses...
  61. pmc Transmission dynamics of an insect-specific flavivirus in a naturally infected Culex pipiens laboratory colony and effects of co-infection on vector competence for West Nile virus
    Bethany G Bolling
    Arthropod Borne and Infectious Diseases Laboratory, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO, USA
    Virology 427:90-7. 2012
    ..from eggs collected in Colorado and discovered that mosquitoes in the colony are naturally infected with Culex flavivirus (CxFV), an insect-specific flavivirus...
  62. pmc Age affects quantity but not quality of antibody responses after vaccination with an inactivated flavivirus vaccine against tick-borne encephalitis
    Karin Stiasny
    Department of Virology, Medical University of Vienna, Vienna, Austria
    PLoS ONE 7:e34145. 2012
    ..age-dependence of functional characteristics of antibody responses induced by vaccination with an inactivated flavivirus vaccine against tick-borne encephalitis (TBE)...
  63. doi Genetic characterization of Bagaza virus (BAGV) isolated in India and evidence of anti-BAGV antibodies in sera collected from encephalitis patients
    Vijay P Bondre
    National Institute of Virology, Pashan, Pune 411 021, India
    J Gen Virol 90:2644-9. 2009
    ..This is the first report of the isolation of BAGV from India. The presence of anti-BAGV neutralizing antibodies suggests that the human population has been exposed to BAGV...
  64. pmc The topology of bulges in the long stem of the flavivirus 3' stem-loop is a major determinant of RNA replication competence
    Li Yu
    Laboratory Vector Borne Viruse Disease, Division of Viral Products, FDA, Bethesda, MD 20892, USA
    J Virol 79:2309-24. 2005
    All flavivirus genomes contain a 3'terminal stem-loop secondary structure (3'SL) formed by the most downstream approximately 100 nucleotides (nt) of the viral RNA...
  65. pmc Flavivirus NS3 and NS5 proteins interaction network: a high-throughput yeast two-hybrid screen
    Marc Le Breton
    Inserm Unit 851, Lyon, France
    BMC Microbiol 11:234. 2011
    The genus Flavivirus encompasses more than 50 distinct species of arthropod-borne viruses, including several major human pathogens, such as West Nile virus, yellow fever virus, Japanese encephalitis virus and the four serotypes of dengue ..
  66. pmc cis- and trans-acting elements in flavivirus RNA replication
    A A Khromykh
    Sir Albert Sakzewski Virus Research Centre, Royal Children s Hospital, Brisbane, Queensland 4029, Australia
    J Virol 74:3253-63. 2000
    Most of the seven flavivirus nonstructural proteins (NS1 to NS5) encoded in the distal two-thirds of the RNA positive-sense genome are believed to be essential components of RNA replication complexes...
  67. doi Neuroinvasive flavivirus infections
    Gregorius J Sips
    Department of Medical Microbiology, Molecular Virology Section, University Medical Center Groningen, University of Groningen, The Netherlands
    Rev Med Virol 22:69-87. 2012
    ..This review focuses on the epidemiology, symptomatology, neuropathology, and, specifically, neuropathogenesis of flavivirus-induced human CNS disease...
  68. pmc Transmission of West Nile virus by Culex quinquefasciatus say infected with Culex Flavivirus Izabal
    Rebekah J Kent
    Division of Vector Borne Infectious Diseases, Arbovirus Diseases Branch, Centers for Disease Control and Prevention, Fort Collins, Colorado, USA
    PLoS Negl Trop Dis 4:e671. 2010
    ..The objective of this study was to determine whether or not prior infection with Culex flavivirus (CxFV) Izabal altered the vector competence of Cx...
  69. pmc Sequence comparison and secondary structure analysis of the 3' noncoding region of flavivirus genomes reveals multiple pseudoknots
    R C Olsthoorn
    Institute of Molecular Plant Sciences, Gorlaeus Laboratories, Leiden University, The Netherlands
    RNA 7:1370-7. 2001
    Sequences of 191 flavivirus RNAs belonging to four sero-groups were used to predict the secondary structure of the 3' noncoding region (3' NCR) directly upstream of the conserved terminal hairpin...
  70. ncbi Host range specificity of flaviviruses: correlation with in vitro replication
    G Kuno
    Arboviral Disease Branch, Division Vector Borne Infectious Diseases, Centers for Disease Control and Prevention, P O Box 2087, Fort Collins, CO 80522, USA
    J Med Entomol 44:93-101. 2007
    ..Thus, this assay provides useful, supplementary information regarding host range for those flaviviruses when their natural host range is unknown, ambiguous, or questionable...
  71. ncbi Molecular biology of flaviviruses
    Brett D Lindenbach
    Center for the Study of Hepatitis C, Laboratory of Virology and Infectious Disease, Rockefeller University, New York, New York 10021, USA
    Adv Virus Res 59:23-61. 2003
  72. pmc Secondary structure of the 3' untranslated region of flaviviruses: similarities and differences
    V Proutski
    The Wellcome Trust Centre for the Epidemiology of Infectious Disease, Department of Zoology, University of Oxford, South Parks Road, Oxford OX1 3PS, UK
    Nucleic Acids Res 25:1194-202. 1997
    ..These alternative folding patterns may have roles in the regulation of transcription and translation initiation and in the switch between them...
  73. ncbi Full-length sequencing and genomic characterization of Bagaza, Kedougou, and Zika viruses
    G Kuno
    Arbovirus Diseases Branch, Division of Vector Borne Infectious Diseases, National Center for Zoonotic, Vector Borne, and Enteric Diseases, Centers for Disease Control and Prevention, Fort Collins, CO 80522 2087, USA
    Arch Virol 152:687-96. 2007
    Many members of the genus Flavivirus are the agents of important diseases of humans, livestock, and wildlife...
  74. ncbi Phylogenetic relationships of flaviviruses correlate with their epidemiology, disease association and biogeography
    M W Gaunt
    Pathogen Molecular Biology and Biochemistry Unit, Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK
    J Gen Virol 82:1867-76. 2001
    Phylogenetic analysis of the Flavivirus genus, using either partial sequences of the non-structural 5 gene or the structural envelope gene, revealed an extensive series of clades defined by their epidemiology and disease associations...
  75. pmc A broadly flavivirus cross-neutralizing monoclonal antibody that recognizes a novel epitope within the fusion loop of E protein
    Yong qiang Deng
    State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China
    PLoS ONE 6:e16059. 2011
    ..Cross-reactive antibodies against Flavivirus have been described, but most of them are generally weakly neutralizing...
  76. pmc West Nile and Usutu viruses in mosquitoes in Spain, 2008-2009
    Ana Vazquez
    CNM Instituto de Salud Carlos III, Majadahonda, Spain
    Am J Trop Med Hyg 85:178-81. 2011
    ..This is the first detection and isolation of West Nile virus lineage 1 from mosquitoes in Spain...
  77. doi Usutu virus in wild birds in northern Italy
    G Manarolla
    Dipartimento di Patologia Animale, Igiene e Sanita Pubblica Veterinaria, Universita degli Studi di Milano, Via Celoria 10, 20133 Milano, Italy
    Vet Microbiol 141:159-63. 2010
    ..Sequencing of the amplification products revealed 99.8-100% nucleotide identity of the Italian USUV strains to those from other central European countries...
  78. pmc Structural basis for the preferential recognition of immature flaviviruses by a fusion-loop antibody
    Mickaël V Cherrier
    Department of Biological Sciences, Purdue University, West Lafayette, IN 47907 2054, USA
    EMBO J 28:3269-76. 2009
    ..Here, using X-ray crystallography we have defined the structure of the flavivirus cross-reactive antibody E53 that engages the highly conserved fusion loop of the West Nile virus envelope ..
  79. doi Chapter 2. New insights into flavivirus nonstructural protein 5
    Andrew D Davidson
    Department of Cellular and Molecular Medicine, School of Medical and Veterinary Sciences, University of Bristol, Bristol BS8 1TD, United Kingdom
    Adv Virus Res 74:41-101. 2009
    Disease caused by flavivirus infections is an increasing world health problem...
  80. pmc The 5' and 3' downstream AUG region elements are required for mosquito-borne flavivirus RNA replication
    Peter Friebe
    Division of Infectious Diseases and Vaccinology, School of Public Health, University of California, Berkeley, Berkeley, California 94720 7354, USA
    J Virol 85:1900-5. 2011
    ..Using West Nile virus as a model, we demonstrate that 5'-3' DAR complementarity and HP-3'SL formation are essential for viral RNA replication...
  81. doi Flaviviruses and flavivirus vaccines
    Franz X Heinz
    Department of Virology, Medical University of Vienna, Kinderspitalgasse 15, 1095 Vienna, Austria
    Vaccine 30:4301-6. 2012
    ..Although dengue is the most important flavivirus with respect to global disease incidence, the development and use of vaccines has been hampered so far by the ..
  82. pmc Alkhurma hemorrhagic fever virus in Ornithodoros savignyi ticks
    Remi N Charrel
    Universite de la Mediterranee, Marseille, France
    Emerg Infect Dis 13:153-5. 2007
    ..One Ornithodoros savignyi tick from Saudi Arabia contained AHFV RNA. This is the first direct evidence that AHFV is a tickborne flavivirus and confirms the association between human AHFV cases and tickbite history.
  83. pmc Isolation of a novel species of flavivirus and a new strain of Culex flavivirus (Flaviviridae) from a natural mosquito population in Uganda
    Shelley Cook
    Natural History Museum, Cromwell Road, London SW7 5BD, UK
    J Gen Virol 90:2669-78. 2009
    The genus Flavivirus, which contains approximately 70 single-stranded, positive-sense RNA viruses, represents a unique model for studying the evolution of vector-borne disease, as it includes viruses that are mosquito-borne, tick-borne or ..
  84. ncbi 5'- and 3'-noncoding regions in flavivirus RNA
    Lewis Markoff
    Laboratory of Vector Borne Virus Diseases, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland 20892, USA
    Adv Virus Res 59:177-228. 2003
  85. doi Zoonotic mosquito-borne flaviviruses: worldwide presence of agents with proven pathogenicity and potential candidates of future emerging diseases
    H Weissenbock
    Institute of Pathology and Forensic Veterinary Medicine, Department of Pathobiology, University of Veterinary Medicine, Vienna, Austria
    Vet Microbiol 140:271-80. 2010
    An update on the mosquito-borne flavivirus species including certain subtypes, as listed in the Eighth Report of the International Committee on Taxonomy of Viruses, is given...
  86. pmc A new flavivirus and a new vector: characterization of a novel flavivirus isolated from uranotaenia mosquitoes from a tropical rain forest
    Sandra Junglen
    Research Group Emerging Zoonoses, Robert Koch Institute, Berlin, Germany
    J Virol 83:4462-8. 2009
    A novel flavivirus was isolated from Uranotaenia mashonaensis, a mosquito genus not previously known to harbor flaviviruses. Mosquitoes were caught in the primary rain forest of the Taï National Park, Côte d'Ivoire...
  87. pmc Flavivirus entry receptors: an update
    Manuel Perera-Lecoin
    INSERM U944, CNRS 7212, Laboratoire de Pathologie et Virologie Moléculaire, Hopital Saint Louis, 1 avenue Claude Vellefaux, Paris 75010, France
    Viruses 6:69-88. 2014
    ..Here, we present the currently available knowledge regarding the flavivirus receptors described so far with specific attention to C-type lectin receptors and the phosphatidylserine ..
  88. pmc Nuclear localization of flavivirus RNA synthesis in infected cells
    Pradeep Devappa Uchil
    Department of Microbiology and Cell Biology, Room 254A, Sir C V Raman Avenue, Indian Institute of Science, Bangalore 560012, India
    J Virol 80:5451-64. 2006
    ..these data suggest an absolute requirement for both of the replicase proteins for nucleus-localized synthesis of flavivirus RNA...
  89. doi Barkedji virus, a novel mosquito-borne flavivirus identified in Culex perexiguus mosquitoes, Israel, 2011
    Jolanta Kolodziejek
    Viral Zoonoses, Emerging and Vector Borne Infections Group, Institute of Virology, University of Veterinary Medicine Vienna, Vienna, Austria
    J Gen Virol 94:2449-57. 2013
    Barkedji virus, named after the area of its first identification in Senegal, is a newly discovered flavivirus (FV), for which we propose the abbreviation BJV...
  90. pmc Oral susceptibility of Singapore Aedes (Stegomyia) aegypti (Linnaeus) to Zika virus
    Meizhi Irene Li
    Environmental Health Institute, National Environment Agency, Singapore, Singapore
    PLoS Negl Trop Dis 6:e1792. 2012
    Zika virus (ZIKV) is a little known flavivirus that caused a major outbreak in 2007, in the South-western Pacific Island of Yap. It causes dengue-like syndromes but with milder symptoms...
  91. ncbi Alkhumra virus infection, a new viral hemorrhagic fever in Saudi Arabia
    Tariq A Madani
    Department of Medicine, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia
    J Infect 51:91-7. 2005
    ..A new flavivirus closely related to the tick-borne Kyasanur forest disease virus was isolated...
  92. doi Characterization of a novel flavivirus isolated from Culex (Melanoconion) ocossa mosquitoes from Iquitos, Peru
    Julio Evangelista
    Virology Department, US Naval Medical Research Unit No 6, Lima and Iquitos, Peru
    J Gen Virol 94:1266-72. 2013
    We describe the isolation and characterization of a novel flavivirus, isolated from a pool of Culex (Melanoconion) ocossa Dyar and Knab mosquitoes collected in 2009 in an urban area of the Amazon basin city of Iquitos, Peru...
  93. doi Nonstructural NS1 proteins of several mosquito-borne Flavivirus do not inhibit TLR3 signaling
    Cécile Baronti
    UMR IRD 190, Emergence des Pathologies Virales, Universite de la Mediterranee, Faculte de Medecine de la Timone, 27 Bd Jean Moulin, 13005 Marseille, France
    Virology 404:319-30. 2010
    ..We conclude that NS1 proteins from distinct mosquito-borne flaviviruses do not inhibit TLR3 signaling...
  94. pmc Structure and functionality in flavivirus NS-proteins: perspectives for drug design
    Michela Bollati
    Department of Biomolecular Sciences and Biotechnology, University of Milano, Via Celoria 26, 20133 Milano, Italy
    Antiviral Res 87:125-48. 2010
    ..Besides yellow fever virus, a landmark case in the history of virology, members of the Flavivirus genus, such as West Nile virus and dengue virus, are increasingly gaining attention due to their re-emergence ..
  95. pmc A three-dimensional comparison of tick-borne flavivirus infection in mammalian and tick cell lines
    Danielle K Offerdahl
    Laboratory of Virology, Rocky Mountain Laboratories, NIAID, NIH, Hamilton, Montana, United States of America
    PLoS ONE 7:e47912. 2012
    ..Additionally, we show for the first time 3D flavivirus infection in a vector cell line and the first ET of persistent flavivirus infection.
  96. doi First isolation of Aedes flavivirus in the Western Hemisphere and evidence of vertical transmission in the mosquito Aedes (Stegomyia) albopictus (Diptera: Culicidae)
    Andrew D Haddow
    Institute for Human Infections and Immunity, Center for Emerging Infectious Diseases and Biodefense, Department of Pathology, University of Texas Medical Branch, Galveston, TX 77555 0609, USA
    Virology 440:134-9. 2013
    We report here the first evidence of vertical transmission of Aedes flavivirus (AEFV) and its first isolation in the Western Hemisphere...
  97. ncbi Recent advances in flavivirus antiviral drug discovery and vaccine development
    Debashish Ray
    Wadsworth Center, New York State Department of Health, Albany, New York, USA
    Recent Pat Antiinfect Drug Discov 1:45-55. 2006
    ..Despite an emergence and resurgence of flavivirus-mediated disease, specific therapies are not yet available; however, significant progress has been made toward ..
  98. doi Blood meal analysis, flavivirus screening, and influence of meteorological variables on the dynamics of potential mosquito vectors of West Nile virus in northern Italy
    David Roiz
    Environment and Natural Resources Area, IASMA Research and Innovation Centre, Edmund Mach Foundation, Via E Mach 1, 38010 S Michele all Adige TN, Italy
    J Vector Ecol 37:20-8. 2012
    ..Furthermore, we present the first contribution to understanding the transmission role of Cx. pipiens mosquitoes in Italy by identifying vertebrate hosts to species level...
  99. pmc Point mutations in the West Nile virus (Flaviviridae; Flavivirus) RNA-dependent RNA polymerase alter viral fitness in a host-dependent manner in vitro and in vivo
    Greta A Van Slyke
    The Arbovirus Laboratories, Wadsworth Center, New York State Dept of Health, NY 12159, USA
    Virology 427:18-24. 2012
    ..Our results demonstrate that subtle alteration of the RdRp protein away from the active site can have a significant overall biological effect on WNV fitness, and that this effect can be host-dependent...
  100. ncbi Genome sequence analysis of Tamana bat virus and its relationship with the genus Flavivirus
    X De Lamballerie
    Unite des Virus Emergents, EA3292 IFR48, Universite de la Mediterranee, Faculte de Medecine, 27 Bd J Moulin, F13005 Marseille, France
    J Gen Virol 83:2443-54. 2002
    ..TABV, isolated from the bat Pteronotus parnellii) is currently classified as a tentative species in the genus FLAVIVIRUS: We report here the determination and analysis of its complete coding sequence...
  101. doi Sequence signatures in envelope protein may determine whether flaviviruses produce hemorrhagic or encephalitic syndromes
    Winona C Barker
    Department of Biochemistry and Molecular and Cellular Biology, Georgetown University Medical Center, Washington, DC 20007, USA
    Virus Genes 39:1-9. 2009
    ..These findings should help predicting the disease potential of emerging and re-emerging flaviviruses and understanding the relationship between protein structure and disease outcome...

Research Grants68

  1. Role of an essential RNA chaperone in virus replication
    Peter Nagy; Fiscal Year: 2006
    ..is similar in tombusviruses and numerous other important human and animal viruses (hepatitis C virus, flavivirus, including West Nile virus and dengue virus, pestiviruses) and plant viruses (luteo- and carmoviruses), ..
  2. Cellular Immunity to Category A-C Viruses in Humans
    ROBERT WILLIAM FINBERG; Fiscal Year: 2013
    ..Project 3 will analyze heterologous immunity in flavivirus infections, defining the effects of amino acid variations on cross-reactive T-cell responses in sequential ..
  3. Identification and Analysis of Flavivirus Protease and RNA Helicase Inhibitors
    Radhakrishnan Padmanabhan; Fiscal Year: 2009
    ..inhibitors that will be useful for co-crystallization and structure-function studies that could provide valuable insight into the nature of molecular interactions among flavivirus nonstructural proteins in the flavivirus life cycle.
  4. Thirteenth Annual Meeting of the Rocky Mountain Virology Association
    Joel Rovnak; Fiscal Year: 2013
    ..e. RNA stability and Flavivirus biology...
  5. Development of a Recombinant Tetravalent Dengue Vaccine Based on a Subunit Envelo
    Lynda G Tussey; Fiscal Year: 2013
    ..We have developed a series of flavivirus vaccines based on our clinically proven vaccine platform in three formats, which differ in the site of antigen ..
  6. Cellular Components Involved in Japanese Encephalitis Virus Entry
    Young Min Lee; Fiscal Year: 2013
    ..JEV is a mosquito-borne flavivirus that is closely related to West Nile virus, St...
  7. Fatty Acid Synthase Inhibitors As Broad Spectrum Anti-Flaviviral Therapeutics
    Glenn Randall; Fiscal Year: 2013
    ..The Diamond lab are leaders in flavivirus research and are highly experienced with DENV and West Nile Virus animal models and inhibitor testing...
  8. Alternative regulation of ISGs in WNV-infected cells
    Margo A Brinton; Fiscal Year: 2013
    ..No effective antiviral therapies currently exist for treating individuals with flavivirus infections and flavivirus-induced pathogenesis is not completely understood...
  9. Mosquito Saliva in Dengue Virus Pathogenesis
    REBECCA R RICO-HESSE; Fiscal Year: 2013
    ..Dengue virus is a single-stranded RNA Flavivirus that causes dengue fever (DF), the most prevalent arthropod-borne viral illness in humans...
  10. The effects of hepatitis C virus (HCV) E2 protein on host immunomodulation
    Jack T Stapleton; Fiscal Year: 2013
    ..Based on work in the most closely related human flavivirus (GB virus C;GBV-C), we hypothesize that E2 protein competes with the key proximal lymphocyte-specific protein ..
  11. Characterization of the La Crosse Virus glycoprotein fusion peptide
    SAMANTHA STANDISH SOLDAN; Fiscal Year: 2012
    ..Collectively, these studies suggested that the LACV Gc, like the alphavirus E1 and the flavivirus E, functions as a type II fusion protein...
  12. Rational Development of Candidate Attenuated Flavivirus Vaccines
    Tian Wang; Fiscal Year: 2013
    ..attenuated vaccines, which induce durable protective immunity, are one of the important strategies to control flavivirus diseases and have been very successful at controlling yellow fever (YF) and Japanese encephalitis (JE)...
  13. MOLECULAR MECHANISMS OF ALPHAVIRUS ENTRY AND EXIT
    Margaret Kielian; Fiscal Year: 2013
    ..The long-term goal of this grant is to define the molecular mechanisms of alphavirus and flavivirus entry and exit and the role of host proteins in these processes...
  14. Mechanism of RNA synthesis and 5'-capping by dengue virus NS5 polymerase
    Kyung H Choi; Fiscal Year: 2013
    ..Despite the considerable impact of flavivirus infection on world-wide health, no antiviral therapies are available, and existing flavivirus vaccines are of ..
  15. Innate Immune Recognition Enhances Flavivirus Vaccine Efficacy
    Gregg N Milligan; Fiscal Year: 2010
    ..Vaccines are urgently needed for multiple flavivirus diseases, notably dengue and West Nile encephalitis...
  16. Molecular Mechanisms of Eastern Equine Encephalitis Virus Pathogenesis
    William B Klimstra; Fiscal Year: 2013
    ..sulfate (HS) is a sulfated polysaccharide identified as an attachment receptor for multiple alphavirus and flavivirus laboratory strains but its relevance to viruses in nature is in question because its use as a receptor is ..
  17. A new synergy for flavivirus therapy: RNAi enhancement and viral mutagens
    KATHRYN ALYCE HANLEY; Fiscal Year: 2012
    DESCRIPTION (provided by applicant): The genus Flavivirus, comprising 80 species of single-stranded, positive-sense RNA viruses, includes a large number of globally significant emerging pathogens...
  18. ALTERATIONS IN ADULT NEUROGENESIS DURING WEST NILE VIRUS ENCEPHALITIS
    MICHAEL JOHN VASEK; Fiscal Year: 2013
    ..West Nile virus (WNV), a flavivirus now endemic in North America, is one of several viruses that are able to enter the CNS, infect neurons, and ..
  19. Structure-function studies of alpha- and flaviviruses
    Richard J Kuhn; Fiscal Year: 2010
    The current understanding of alphavirus and flavivirus life cycles at the molecular level is limited because we lack a structural foundation for the viral and host proteins, especially those involved in RNA replication, genome packaging ..
  20. Dengue Virus Genome Encapsidation and Its Interplay with Host Lipid Droplets
    Andrea Gamarnik; Fiscal Year: 2013
    ..The flavivirus capsid is a highly basic protein that binds any nucleic acid, yet only the viral genome is encapsidated...
  21. The West Nile virus methyltransferase
    Hongmin Li; Fiscal Year: 2010
    DESCRIPTION (provided by applicant): Many members of the genus Flavivirus are significant human pathogens. The 5'end of flavivirus plus- sense RNA genome contains a type 1 cap (m7GpppAmG)...
  22. The role of interleukin 22 in West Nile virus pathogenesis in mice
    Penghua Wang; Fiscal Year: 2013
    ..West Nile virus (WNV) is a neurotropic ssRNA flavivirus that has caused over 1000 deaths in the United States since 1999...
  23. Dendritic Cell Targeting Enhances Flavivirus Vaccine Efficacy
    Gregg Milligan; Fiscal Year: 2009
    Description (provided by applicant): Viruses in the flavivirus family populate all three of NIAID's Category A, B, and C lists of viruses...
  24. A Small Animal Model for Viscerotropic Disease to Improve Yellow Fever Vaccine
    KATHERINE D RYMAN; Fiscal Year: 2013
    ..by the use of 17D vaccine virus to generate chimeric viruses containing the structural protein genes of one flavivirus (e.g. Japanese encephalitis) in a 17D vaccine virus backbone...
  25. Does Dengue Virus Suppress RNA Interference in its Mosquito Vector?
    KATHRYN ALYCE HANLEY; Fiscal Year: 2010
    ..about the potential for harnessing RNAi using synthetic short interfering RNA's (siRNA's) to control flavivirus infection and transmission, but nothing is known about the ability of flaviviruses to inhibit RNAi, nor has the ..
  26. ISG CONTROL OF FLAVIVIRUS INFECTION
    Michael S Diamond; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Members of Flavivirus genus are the most important arthropod-borne viruses causing disease in humans...
  27. Inhibition and mechanism of flavivirus methyltransferase
    Hongmin Li; Fiscal Year: 2013
    ..However, no clinically approved antiviral therapy is available for treatment of flavivirus infections...
  28. CHEMOKINE-MEDIATED NEUROPROTECTION AND REPAIR DURING WNV ENCEPHALITIS
    ROBYN SUE KLEIN; Fiscal Year: 2013
    ..Murine WNV infection is well-accepted flavivirus encephalitis model whereby subcutaneous infection with low inoculum of virulent strains leads to neuroinvasive ..
  29. TAM Receptors and Flavivirus Infection
    GREG E LEMKE; Fiscal Year: 2013
    ..S (ProS) - have the ability to attach both to the phospholipid phosphatidylserine, expressed on the surface of flavivirus particles, and also to their cognate TAM receptor...
  30. High resolution electron cryo-microscope at Brandeis University.
    Nikolaus Grigorieff; Fiscal Year: 2009
    ..entry will be studied using a model system based on "small virus-like particles" isolated by expression of the flavivirus prM and E proteins...
  31. Modulation of arboviruses in mosquitoes
    Kevin M Myles; Fiscal Year: 2012
    ..RNAi-based modulation of alphaviruses, and 3) determine the role of an RNAi-based immune response in modulating flavivirus infections in natural vector hosts...
  32. Therapy of Dengue with Modified Antibodies in Humanized Mice
    REBECCA R RICO-HESSE; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Dengue virus (DV) is a single-stranded RNA Flavivirus that causes dengue fever (DF), the most prevalent arthropod-borne viral illness in humans...
  33. Structure and Function of Flaviviruses
    Michael G Rossmann; Fiscal Year: 2013
    ..We will examine some of the many cell surface molecules that have been tentatively shown to be flavivirus receptors by binding recombinantly produced fragments of potential receptor molecule to the virus...
  34. Renal And Neurological Outcomes In Chronically Infected West Nile Virus Patients
    Kristy Murray; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): West Nile virus (WNV) is an important emerging flavivirus resulting in acute and sometimes fatal encephalitis in humans...
  35. Development of an H2O2-Inactivated Dengue Virus Vaccine
    Mark K Slifka; Fiscal Year: 2013
    ..We have developed an innovative vaccine platform that has shown in vivo efficacy against lethal flavivirus infection in two independent models (West Nile virus and Yellow Fever virus) and in this application we will ..
  36. Discovery of antiviral agents targeting dengue viral translation
    JOSEPH MATTHEW COLACINO; Fiscal Year: 2012
    ..Currently, no vaccines or specific antiviral therapy are available against DENV infection. DENV is a flavivirus that employs a unique molecular mechanism for the initiation of viral protein translation which is regulated by ..
  37. Molecular Mechanisms of Rhabdovirus Entry
    Sean P J Whelan; Fiscal Year: 2013
    ..These fusion proteins fall into three classes as exemplified by influenza hemagglutinin (class I), flavivirus envelope proteins (class II) and rhabdovirus glycoproteins (class III)...
  38. Antiviral therapeutics for flavivirus infections
    CHELSEA BYRD OLSEN; Fiscal Year: 2013
    ..In this application chemical analogs of selected quality hits will be synthesized to improve the properties of the compounds leading to the nomination of a preclinical candidate which will enter into IND-enabling toxicology. ..
  39. Nonstructural roles of capsid proteins in virus replication
    TERYL FREY; Fiscal Year: 2009
    ..We have evidence that the capsid protein of West Nile virus (Flavivirus family) enhances the replication of a West Nile virus replicon, indicating that C involvement in viral RNA ..
  40. A High-Throughput Assay for Probes of the Flavivirus RNA Guanylyltransferase
    Brian Geiss; Fiscal Year: 2012
    ..West Nile is a major medical and socio-economic problem worldwide, yet effective antiviral therapeutics to treat flavivirus infection are not currently available...
  41. Impact of Aging on Memory B Cell and Humoral Immunity with West Nile Virus
    JUSTIN RICHNER; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): West Nile Virus (WNV) is an emerging mosquito-born flavivirus that can lead to fatal encephalitis in several vertebrate animal species...
  42. Immunotherapeutics for Treatment of Flavivirus Infection
    Erol Fikrig; Fiscal Year: 2010
    ..a synergistic combination of two antibodies for enhanced neutralization and therapy against WNV and other flavivirus infections...
  43. Membrane Rearrangements in Flavivirus Infected Cells
    Richard J Kuhn; Fiscal Year: 2010
    ..approach to investigate the molecular mechanisms that drive intracellular membrane rearrangements during flavivirus infection...
  44. Discovery and development of broad spectrum anti-flaviviral drugs
    Timothy A Haystead; Fiscal Year: 2013
    ..2) Chemical screens for inhibitors of flavivirus replication...
  45. Regulation of West Nile virus replication.
    Margo A Brinton; Fiscal Year: 2012
    ..investigator's lab show that the highly conserved cell stress granule proteins, TIAR and TIA-1, facilitate flavivirus plus strand RNA synthesis by interacting with the 3'terminal stem loop of the viral complementary RNA...
  46. Pacific NorthWest Regional Center of Excellence (PNWRCE)
    Jay A Nelson; Fiscal Year: 2013
    ..a P01 is focused on defining innate immune mechanisms, therapeutic targets, and antiviral compounds that limit flavivirus infection and pathogenesis...
  47. Molecular Mechanisms of Rhabdovirus Entry
    Sean Whelan; Fiscal Year: 2009
    ..These fusion proteins fall into three classes as exemplified by influenza hemagglutinin (class I), flavivirus envelope proteins (class II) and rhabdovirus glycoproteins (class III)...
  48. Design of a human monoclonal antibody-informed dengue vaccine
    Sharon Isern; Fiscal Year: 2013
    ..The first specific aim is to design flavivirus E protein chimeras containing DENV epitopes, and characterize their folding, assembly, and functionality...
  49. Flavivirus Infections: Pathogenesis and Prevention
    Alan L Rothman; Fiscal Year: 2013
    ..The findings from these proposed research studies should have broad basic science as well as clinical and public health implications. ..
  50. Gammadelta T cell Regulation of Adaptive Immunity in West Nile Virus Infection
    Tian Wang; Fiscal Year: 2012
    ..understanding of host immunity against WNV;but also will provide critical basic insights for new strategies in flavivirus vaccine development...
  51. Development of Novel Small Molecule Flavivirus Inhibitors
    ERIC W BARKLIS; Fiscal Year: 2010
    ..The development of TYT-1-based antivirals will provide a new approach to the treatment of flavivirus infections, and will complement vaccine approaches...
  52. Imino sugars for flavivirus infections of bioterror
    Timothy M Block; Fiscal Year: 2010
    ..the imino sugar, N, -nonyl-deoxynorjirimycin (NN-DNJ) has been shown to have efficacy in animal models of flavivirus infection...
  53. Host factor DNAJC14 as a novel target for broad spectrum anti-flavivirus activity
    Margaret R Macdonald; Fiscal Year: 2010
    ..We identified a host protein, DNAJC14, as inhibitory to cell death caused by the flavivirus, yellow fever virus and will investigate the spectrum and mechanism of action of DNAJC14 as an anti- flavivirus ..
  54. Genetic determinants of adaptation of two flaviviruses
    Laura D Kramer; Fiscal Year: 2013
    ..provided by applicant): The emergence and rapid biogeographic expansion of West Nile Virus (WNV;Flaviviridae;Flavivirus) throughout the western hemisphere, as well as the re-emergence of other medically significant viruses, ..
  55. GBV-C effects on CD4 activation and expansion
    Jack T Stapleton; Fiscal Year: 2012
    ..others found an association between infection with GB virus C (GBV-C) infection, a common, nonpathogenic human flavivirus, and prolonged survival in several cohorts of HIV-infected people...
  56. Defining the role of the Inflammasome in immunity against Flavivirus Infection
    HILARIO RAMOS; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): West Nile Virus (WNV) is as model flavivirus and an NIAID Category B infectious/emerging agent...
  57. Dengue and West Nile Viral Protease Inhibitors
    Radhakrishnan Padmanabhan; Fiscal Year: 2005
    The mosquito-borne flavivirus family includes human pathogens such as yellow fever virus (YFV), West Nile virus (WNV), and the four serotypes of Dengue (DEN) viruses...
  58. YELLOW FEVER 17D-BASED CHIMERIC FLAVIVIRUS VACCINES
    William Wold; Fiscal Year: 2002
    ....
  59. Broad-Spectrum Therapeutic Human Antibodies for Dengue Virus Infections
    Yorgo Modis; Fiscal Year: 2006
    ..by applicant): The overall goal of this project is to develop antibody therapeutics to treat life-threatening flavivirus infections...
  60. HOST COMPETENCE FACTORS AFFECT ARBOVIRUS TRANSMISSION
    William Reisen; Fiscal Year: 2003
    ..mosquito, Culex tarsalis, to infection with the Alphavirus western equine encephalomyelitis virus (WEE) and the Flavivirus St...
  61. MOLECULAR ANALYSIS OF ALPHAVIRUS MEMBRANE FUSION PROTEIN
    Margaret Kielian; Fiscal Year: 2006
    ..Striking similarities exist between the native structures of the alphavirus and flavivirus membrane fusion proteins...
  62. Molecular Evolution of flavivirus-resistance in mice
    Stephane Boissinot; Fiscal Year: 2005
    The Flavivirus genus contains many viruses of considerable medical importance including the dengue virus, the yellow fever virus and the West Nile virus...
  63. DETERMINANTS OF HEPATITIS C & E MORBIDITY IN EGYPT
    George Strickland; Fiscal Year: 2004
    ..patients have now been categorized into those infected with hepatitis C virus (HCV), a parenterally transmitted flavivirus, and hepatitis E virus (HEV), a fecal-oral transmitted "hepatitis E-like virus...
  64. DETERMINANTS OF HEPATITIS C & E MORBIDITY IN EGYPT
    George Strickland; Fiscal Year: 2002
    ..patients have now been categorized into those infected with hepatitis C virus (HCV), a parenterally transmitted flavivirus, and hepatitis E virus (HEV), a fecal-oral transmitted "hepatitis E-like virus...
  65. Anti-Flavivirus Immunotherapeutics
    EILEEN NAKANO; Fiscal Year: 2001
    ..More than 250,000 annual cases of severe dengue disease are reported each year. ..
  66. Targets for Drug Design In Flaviviral Infection
    Kurt Krause; Fiscal Year: 2004
    ..There are no currently effective medications for the treatment of these flavivirus infections...
  67. Identifying Epitopes Recognized by Influenza and Flavivirus.
    William Kwok; Fiscal Year: 2013
    ..abstract_text> ..