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| potyvirusSummarySummary: A large genus of plant viruses of the family POTYVIRIDAE which infect mainly plants of the Solanaceae. Transmission is primarily by aphids in a non-persistent manner. The type species is potato virus Y. Top Publications
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Recessive resistance to plant virusesV Truniger
Centro de Edafología y Biología Aplicada del Segura CEBAS, Consejo Superior de Investigaciones Cientificas CSIC, Apdo Correos 164, 30100 Espinardo Murcia, Spain
Adv Virus Res 75:119-59. 2009..We review actual and probable recessive resistance mechanisms, and bring the chapter to a close by summarizing the current state-of-the-art and offering perspectives on potential future developments...
Formation of complexes at plasmodesmata for potyvirus intercellular movement is mediated by the viral protein P3N-PIPOTaiyun Wei
Southern Crop Protection and Food Research Centre, Agriculture and Agri Food Canada, London, Ontario, Canada
PLoS Pathog 6:e1000962. 2010..Previous genetic and ultrastructural data revealed that the potyvirus cyclindrical inclusion (CI) protein is directly involved in cell-to-cell movement, likely through the formation ..
Distribution of fitness and virulence effects caused by single-nucleotide substitutions in Tobacco Etch virusPurificación Carrasco
Instituto de Biología Molecular y Celular de Plantas CSIC UPV, Avenida de los Naranjos s n, 46022 Valencia, Spain
J Virol 81:12979-84. 2007..Here we used site-directed mutagenesis to create a collection of 66 clones of Tobacco etch potyvirus, each carrying a different, randomly chosen, single-nucleotide substitution...
Genetic evidence for an essential role for potyvirus CI protein in cell-to-cell movementJ C Carrington
Institute of Biological Chemistry, Washington State University, Pullman 99164, USA
Plant J 14:393-400. 1998The potyvirus cylindrical inclusion (CI) protein, an RNA helicase required for genome replication, was analyzed genetically using alanine-scanning mutagenesis...
A cysteine-rich plant protein potentiates Potyvirus movement through an interaction with the virus genome-linked protein VPgP Dunoyer
John Innes Centre, Norwich NR4 7UH, United Kingdom
J Virol 78:2301-9. 2004..The factor, called Potyvirus VPg-interacting protein (PVIP), is a plant-specific protein with homologues in all the species examined, i.e...
Point mutations in helper component protease of clover yellow vein virus are associated with the attenuation of RNA-silencing suppression activity and symptom expression in broad beanM L M Yambao
Pathogen Plant Interactions Group, Plant Breeding Science, Graduate School of Agriculture, Hokkaido University, Sapporo, Japan
Arch Virol 153:105-15. 2008Helper component protease (HC-Pro) is a potyvirus-encoded multifunctional protein and a major determinant of symptom expression in a susceptible plant...
Molecular dissection of the potato virus Y VPg virulence factor reveals complex adaptations to the pvr2 resistance allelic series in pepperValérie Ayme
INRA, UR407 Pathologie Végétale, F 84143 Montfavet, France
J Gen Virol 88:1594-601. 2007..They provide a better understanding of the variable levels of durability of the resistance conferred by the different pvr2 alleles, and have important consequences for a durable management of the resistances...
Transcriptional changes and oxidative stress associated with the synergistic interaction between Potato virus X and Potato virus Y and their relationship with symptom expressionAlberto García-Marcos
Departamento de Biología de Plantas, Centro de Investigaciones Biologicas, CSIC, Ramiro de Maeztu 9, 28040 Madrid, Spain
Mol Plant Microbe Interact 22:1431-44. 2009..Interestingly, expression of genes encoding oxylipin biosynthesis was uniquely upregulated by the synergistic infection. Virus-induced gene silencing of alpha-dioxygenase1 delayed cell death during PVX-PVY infection...
Spatial analysis of arabidopsis thaliana gene expression in response to Turnip mosaic virus infectionChunling Yang
Department of Plant Pathology, Iowa State University, Ames, Iowa 50011, USA
Mol Plant Microbe Interact 20:358-70. 2007..The functions of these downregulated genes are consistent with viral symptoms, such as chlorosis and stunted growth, providing new insight into mechanisms of pathogenesis...
Structural basis for the substrate specificity of tobacco etch virus proteaseJason Phan
Macromolecular Crystallography Laboratory, Center for Cancer Research, NCI-Frederick, National Institutes of Health, Frederick, Maryland 21702-1201, USA
J Biol Chem 277:50564-72. 2002..Analysis of the protein-ligand interactions helps to delineate the structural determinants of substrate specificity and provides guidance for reengineering the enzyme to further improve its utility for biotechnological applications...
Silencing on the spot. Induction and suppression of RNA silencing in the Agrobacterium-mediated transient expression systemL K Johansen
Institute of Biological Chemistry, Washington State University, Pullman, Washington 99164-6340, USA
Plant Physiol 126:930-8. 2001..They also indicate that RNA silencing may be a significant limitation to expression of genes in the Agrobacterium-mediated transient assay but that this limitation can be overcome by using RNA silencing suppressors...
Natural variation and functional analyses provide evidence for co-evolution between plant eIF4E and potyviral VPgCarine Charron
INRA UR1052, Genetics and Breeding of Fruits and Vegetables, Domaine St Maurice, BP94, F 84143 Montfavet, France
Plant J 54:56-68. 2008..amino acid changes in the central part of the viral genome-linked protein (VPg) are responsible for the potyvirus's ability to overcome eIF4E-mediated resistance...
Intrinsic disorder in Viral Proteins Genome-Linked: experimental and predictive analysesEugenie Hebrard
UMR 1097 Résistance des Plantes aux Bio agresseurs, IRD, CIRAD, Universite de Montpellier II, BP 64501, 34394 Montpellier Cedex 5, France
Virol J 6:23. 2009..To date, structural data are still limited to small picornaviral VPgs. Recently three phytoviral VPgs were shown to be natively unfolded proteins...
Discussion paper: The naming of Potato virus Y strains infecting potatoR P Singh
Potato Research Centre, Agriculture and Agri Food Canada, New Brunswick, Canada
Arch Virol 153:1-13. 2008..A more systematic investigation and characterization of PVY from potato at the biological and molecular levels should eventually result in a biologically meaningful genetic strain concept...
An unusual structure at one end of potato potyvirus particlesLesley Torrance
Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, UK
J Mol Biol 357:1-8. 2006..Our results show that some of the potyvirus particles contain a protruding tip at one end of the virus particles, which is presumably associated with the 5'-..
HcPro, a multifunctional protein encoded by a plant RNA virus, targets the 20S proteasome and affects its enzymic activitiesLionel Ballut
, , 24 avenue des Landais, , France
J Gen Virol 86:2595-603. 2005..evidence that a plant virus protein, the helper component-proteinase (HcPro) of Lettuce mosaic virus (LMV; genus Potyvirus), interferes with the 20S proteasome ribonuclease is reported here...
Molecular characterization and detection of recombinant isolates of potato virus Y from ChinaXinxi Hu
Hunan Provincial Engineering Research Center for Potatoes, College of Horticulture and Landscape, Hunan Agricultural University, Changsha, Hunan 410128, China
Arch Virol 154:1303-12. 2009..Further analysis of these samples using enzyme-linked immunosorbent assay and bioassay revealed that 'HN2' possesses a PVY(O) serotype, a PVY(N) pathotype in tobacco and a PVY(NTN) pathotype in potato...
Adaptation of tobacco etch potyvirus to a susceptible ecotype of Arabidopsis thaliana capacitates it for systemic infection of resistant ecotypesJasna Lalić
Instituto de Biologia Molecular y Celular de Plantas, Consejo Superior de Investigaciones Científicas UPV, 46022 Valencia, Spain
Philos Trans R Soc Lond B Biol Sci 365:1997-2007. 2010..However, empirical tests of this hypothesis are scarce. Arabiodpsis thaliana--tobacco etch potyvirus (TEV) provides an experimentally suitable pathosystem to explore the interplay between genetic variation in host'..
Phosphorylation of the potyvirus capsid protein by protein kinase CK2 and its relevance for virus infectionKonstantin I Ivanov
Institute of Biotechnology, University of Helsinki, FIN 00014 Helsinki, Finland
Plant Cell 15:2124-39. 2003..These results suggest that the phosphorylation of PVA CP by CK2 plays an important regulatory role in virus infection...
Cylindrical inclusion protein of potato virus A is associated with a subpopulation of particles isolated from infected plantsRasa Gabrenaite-Verkhovskaya
Department of Applied Chemistry and Microbiology, FIN 00014 University of Helsinki, Finland
J Gen Virol 89:829-38. 2008..The results are discussed in the context of a model for CI-mediated functions...
Application of game theory to the interaction between plant viruses during mixed infectionsSusana Martin
Instituto de Biología Molecular y Celular de Plantas CSIC UPV, 46022 Valencia, Spain
J Gen Virol 90:2815-20. 2009..Cauliflower mosaic caulimovirus (CaMV) has often been found in mixed infections with turnip mosaic potyvirus (TuMV) in plants of the genus Brassica...
The HC-pro protein of potato virus Y interacts with NtMinD of tobaccoYongsheng Jin
State Key Laboratory of Agro Biotechnology, China Aricultural University, Beijing, P R China
Mol Plant Microbe Interact 20:1505-11. 2007....
Coordinated and selective recruitment of eIF4E and eIF4G factors for potyvirus infection in Arabidopsis thalianaValerie Nicaise
Interactions Plante Virus IPV, UMR 1090, INRA Bordeaux 2, BP 81, F 33883 Villenave d Ornon, France
FEBS Lett 581:1041-6. 2007..analyses of Arabidopsis mutants knocked-out for At-eIF4G genes showed that eIF4G factors are indispensable for potyvirus infection...
Turnip mosaic virus RNA replication complex vesicles are mobile, align with microfilaments, and are each derived from a single viral genomeSophie Cotton
Department of Plant Science, McGill University, 21, 111 Lakeshore, Ste Anne de Bellevue, Quebec H9X 3V9, Canada
J Virol 83:10460-71. 2009..A single-genome origin and presence of protein synthetic machinery components suggest that translation of viral RNA is taking place within the vesicle...
Involvement of the cylindrical inclusion (CI) protein in the overcoming of an eIF4E-mediated resistance against Lettuce mosaic potyvirusAnas Abdul-Razzak
Interactions Plante Virus, UMR GDPP 1090, INRA Université de Bordeaux 2, BP 81, F 33883 Villenave d Ornon, France
Mol Plant Pathol 10:109-13. 2009..Site-directed mutagenesis pinpointed a key role of the amino acid at position 621 in the virulence. This is the first example of the involvement of a potyviral CI protein in the breaking of an eIF4E-mediated resistance...
Biochemical and genetic evidence for interactions between potato A potyvirus-encoded proteins P1 and P3 and proteins of the putative replication complexA Merits
Viikki Biocenter, University of Helsinki, Helsinki, FIN 00014, Finland
Virology 263:15-22. 1999Interactions of the first and third proteins (P1 and P3) of the potato A potyvirus (PVA) with the other six main proteins of PVA were studied using Escherichia coli-expressed recombinant proteins in two in vitro interaction assays and a ..
Mutational analysis of the putative pipo of soybean mosaic virus suggests disruption of PIPO protein impedes movementR H Wen
Department of Entomology and Plant Pathology, The University of Tennessee, Knoxville, TN 37996, USA
Virology 400:1-7. 2010The presence of a small open reading frame embedded in the P3 cistron of potyvirus turnip mosaic virus, termed "pipo," was recently discovered. We have now studied the putative pipo of soybean mosaic virus (SMV)...
Genomic variability in Potato potyvirus Y (PVY): evidence that PVY(N)W and PVY(NTN) variants are single to multiple recombinants between PVY(O) and PVY(N) isolatesL Glais
UMR-BiO3P, INRA, , Rennes-Le Rheu, France
Arch Virol 147:363-78. 2002..Nevertheless, multiple recombination events observed in the PVY(NTN) genome may play a role in the latter phenomenon...
Sequential recruitment of the endoplasmic reticulum and chloroplasts for plant potyvirus replicationTaiyun Wei
Southern Crop Protection and Food Research Centre, AAFC, 1391 Sandford Street, London, Ontario N5V 4T3, Canada
J Virol 84:799-809. 2010..that induces the formation of 6K-containing membranous vesicles at endoplasmic reticulum (ER) exit sites for potyvirus genome replication...
Fluorescent labelling reveals spatial separation of potyvirus populations in mixed infected Nicotiana benthamiana plantsChristof Dietrich
Institute of Plant Diseases and Plant Protection, University of Hannover, , 30419 Hannover, Germany
J Gen Virol 84:2871-6. 2003..Plants infected by any of the PVX/potyvirus combinations exhibited synergistic symptoms and large numbers of cells were doubly infected...
Functional dissection of naturally occurring amino acid substitutions in eIF4E that confers recessive potyvirus resistance in plantsInhwa Yeam
Department of Plant Breeding and Genetics, College of Agriculture and Life Sciences, Cornell University, Ithaca, NY 14853, USA
Plant Cell 19:2913-28. 2007..Overexpression of the Capsicum-eIF4E protein containing the G107R amino acid substitution in Solanum lycopersicum indicated that this polymorphism alone is sufficient for the acquisition of resistance against several TEV strains...
Role of plant virus movement proteinsMichael Taliansky
Scottish Crop Research Institute, Invergowrie, Dundee, DD2 5DA, UK
Methods Mol Biol 451:33-54. 2008..In this review, we focus on the functions and properties of different classes of MPs encoded by RNA containing plant viruses...
Rapid diagnosis of plant virus diseases by transmission electron microscopyBernd Zechmann
University of Graz, Institute of Plant Sciences, Schubertstrasse 51, 8010 Graz, Austria
J Virol Methods 162:163-9. 2009..These methods will contribute towards a rapid and clear identification of virus diseases of plants and will be useful for diagnostic purposes in agriculture and in plant phytopathology...
The Tobacco etch virus P3 protein forms mobile inclusions via the early secretory pathway and traffics along actin microfilamentsXiaoyan Cui
College of Plant Protection, Northwest A and F University, Yangling, Shaanxi 712100, P R China
Virology 397:56-63. 2010..Taken together, these data support previous suggestions that P3 may play dual roles in virus movement and replication...
Amino acid substitutions within the Cys-rich domain of the tobacco etch potyvirus HC-Pro result in loss of transmissibility by aphidsC Llave
, , CSIC, Madrid, Spain
Arch Virol 147:2365-75. 2002We examined the role of several amino acid residues located at the N-terminus of the tobacco etch potyvirus (TEV) helper component-proteinase (HC-Pro) in virus transmissibility by aphids...
Evidence for diversifying selection in Potato virus Y and in the coat protein of other potyvirusesBenoit Moury
Station de Pathologie Vegetale, Institut National de la Recherche Agronomique, F 84143 Montfavet cedex, France
J Gen Virol 83:2563-73. 2002..their biological functions and is essential to an understanding of the evolutionary constraints exerted on the potyvirus genome...
Helper component-proteinase (HC-Pro) protein of Papaya ringspot virus interacts with papaya calreticulinWentao Shen
Key Biotechnology Laboratory for Tropical Crops, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology ITBB, Chinese Academy of Tropical Agriculture Sciences CATAS, 4 Xueyuan Road, Haikou, 571101, China
Mol Plant Pathol 11:335-46. 2010....
Molecular characterisation of an isolate of Dasheen mosaic virus from Zantedeschia aethiopica in China and comparisons in the genus PotyvirusJ Chen
College of Life Science, Zhejiang University, Hangzhou, China
Arch Virol 146:1821-9. 2001..The 9991 nucleotide genome was typical of the genus Potyvirus and phylogenetic analysis showed it to be a member of the Bean common mosaic virus subgroup...
Interaction between potyvirus P3 and ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO) of host plantsLin Lin
State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China
Virus Genes 43:90-2. 2011..It is possible that the potyvirus P3 protein affects the normal functions of RubisCO which thus contributes to symptom development.
HC-Pro protein of sugar cane mosaic virus interacts specifically with maize ferredoxin-5 in vitro and in plantaYu Qin Cheng
Department of Plant Pathology and State Key Laboratory of Agro Biotechnology, China Agricultural University, Beijing 100094, PR China
J Gen Virol 89:2046-54. 2008....
Cellular localisation of calcium ions during potato hypersensitive response to Potato virus YKatarzyna Otulak
Department of Botany, Faculty of Agriculture and Biology, Warsaw University of Life Sciences SGGW, ul Nowoursynowska 159, 02 776 Warsaw, Poland katarzyna
Micron 42:381-91. 2011..The precipitates of calcium antimonate were detected near the nuclear envelope, inside karyolymph and along tracheary elements, especially when virus particles were present inside...
Structural flexibility allows the functional diversity of potyvirus genome-linked protein VPgKimmo I Rantalainen
Department of Food and Environmental Sciences, University of Helsinki, Finland
J Virol 85:2449-57. 2011..Taken together, our experimental data support the features presented in the model and the idea that the functional diversity is attributable to structural flexibility...
Characterisation of Potato virus Y nnp strain inducing veinal necrosis in pepper: a naturally occurring recombinant strain of PVYA Fanigliulo
Dipartimento di Biologia, Difesa e Biotecnologie Agro-Forestali, , Potenza, Italy
Arch Virol 150:709-20. 2005....
Complete nucleotide sequence of a potato isolate of strain group C of Potato virus Y from 1938Annette M Dullemans
Plant Research International, Part of Wageningen UR University and Research Centre, Wageningen, The Netherlands
Arch Virol 156:473-7. 2011..This is the first full-length sequence of a PVY (C) isolate from potato that belongs to the C1 phylogenetic subgroup, which was previously thought to exclusively contain non-potato isolates...
Molecular resolution of a complex of potyviruses infecting solanaceous crops at the centre of origin in PeruC Spetz
Department of Plant Biology and Forest Genetics, Genetics Centre, SLU, PO Box 7080, SE 750 07 Uppsala, Sweden
J Gen Virol 84:2565-78. 2003..Many potyviruses (genus Potyvirus) that infect these crops were described 20-30 years ago...
Stability of Tobacco etch virus infectious clones in plasmid vectorsLeonor C Bedoya
Instituto de Biología Molecular y Celular de Plantas CSIC Universidad Politécnica de Valencia, Avenida de los Naranjos, 46022 Valencia, Spain
Virus Res 149:234-40. 2010..Using the different inocula produced from the three new plasmids the TEV infectivity was also compared. The results showed that agroinoculation is the most effective inoculation method and is where symptoms unfold earlier...
Characterization of Potato virus Y (PVY) molecular determinants involved in the vein necrosis symptom induced by PVYN isolates in infected Nicotiana tabacum cv. XanthiMichel Tribodet
INRA/ENSA, , Domaine de la Motte BP 35327, F-35653 Le Rheu Cedex, France
J Gen Virol 86:2101-5. 2005..Site-directed mutagenesis was used to confirm that these two HC-Pro residues are involved in the TVN phenotype...
Potyvirus terminal protein VPg, effector of host eukaryotic initiation factor eIF4ER Grzela
Institute of Biochemistry and Biophysics (IBB, Polish Academy of Sciences, Warsaw, Poland
Biochimie 88:887-96. 2006b>Potyvirus RNA contains at the 5' end a covalently linked virus-encoded protein VPg, which is required for virus infectivity...
Estimation of the number of virus particles transmitted by an insect vectorBenoit Moury
Institut National de la Recherche Agronomique, UR407, Pathologie Végétale, F 84143 Montfavet, Cedex, France
Proc Natl Acad Sci U S A 104:17891-6. 2007..Such narrow bottlenecks emphasize the strength of stochastic events acting on virus populations, and we illustrate, in modeling virus emergence, why estimating this parameter is important...
The role of the coat protein region in symptom formation on Physalis floridana varies between PVY strainsAgnes Bukovinszki
Agricultural Biotechnology Center, Godollo, Hungary
Virus Res 127:122-5. 2007..The results suggest that symptom determinants may be different even between strains of the same virus species in a particular host...
Specific differentiation of recombinant PVY(N:O) and PVY(NTN) isolates by multiplex RT-PCRXianzhou Nie
Potato Research Centre, Agriculture and Agri-Food Canada, P.O. Box 20280, Fredericton, NB, Canada E3B 4Z7
J Virol Methods 113:69-77. 2003..The cDNA prepared by random primer plus oligo(dT) increased the overall band intensity...
Evolution of North American PVY(NTN) strain Tu 660 from local PVY(N) by mutation rather than recombinationXianzhou Nie
Potato Research Centre, Agriculture and Agri Food Canada, PO Box 20280, 850 Lincoln Road, Fredericton, New Brunswick, Canada E3B 4Z7
Virus Genes 26:39-47. 2003..The non-recombinant nature of NA-PVY(NTN) Tu 660 strongly suggests that the recombinant structure of genome is not a necessary prerequisite for the PTNRD phenotype...
The production of a genus-specific recombinant antibody (scFv) using a recombinant potyvirus proteaseMichael Hust
Lehrgebiet Molekulargenetik, , , D-30419 Hannover, Germany
J Virol Methods 106:225-33. 2002..Therefore, the antibody scFv102 has the potential of becoming a general tool to detect potyvirus infections in different plant species.
Pepper yellow mosaic virus, a new potyvirus in sweetpepper, Capsicum annuumA K Inoue-Nagata
Embrapa Genetic Resources and Biotechnology, Brasilia DF, Brazil
Arch Virol 147:849-55. 2002A potyvirus was found causing yellow mosaic and veinal banding in sweetpepper in Central and Southeast Brazil...
Evolutionary optimization of peptide substrates for proteases that exhibit rapid hydrolysis kineticsKevin T Boulware
Department of Chemical Engineering, University of California Santa Barbara, 93106, USA
Biotechnol Bioeng 106:339-46. 2010....
Recessive resistance in Pisum sativum and potyvirus pathotype resolved in a gene-for-cistron correspondence between host and virusI E Johansen
Biotechnology Group, Danish Institute of Agricultural Sciences, Thorvaldsensvej 40, DK 1871 Frederiksberg C, Denmark
J Virol 75:6609-14. 2001Pea seed-borne mosaic potyvirus (PSbMV) isolates are divided into pathotypes P-1, P-2, and P-4 according to their infection profile on a panel of Pisum sativum lines. P...
Biogenesis of cytoplasmic membranous vesicles for plant potyvirus replication occurs at endoplasmic reticulum exit sites in a COPI- and COPII-dependent mannerTaiyun Wei
Southern Crop Protection and Food Research Centre, AAFC, 1391 Sandford Street, London, Ontario N5V 4T3, Canada
J Virol 82:12252-64. 2008..Previously, a 6-kDa potyvirus membrane protein (6K) was shown to interact with the endoplasmic reticulum (ER) and to induce the formation of ..
Cap-independent translation conferred by the 5' leader of tobacco etch virus is eukaryotic initiation factor 4G dependentD R Gallie
Department of Biochemistry, University of California, Riverside, California 92521 0129, USA
J Virol 75:12141-52. 2001..TEV IRES activity was enhanced moderately by the poly(A)-binding protein. These observations suggest that the TEV IRES directs cap-independent translation through a mechanism that involves eIF4G specifically...
Covalent immobilization of tobacco-etch-virus NIa protease: a useful tool for cleavage of the histidine tag of recombinant proteinsAna Cristina Puhl
Centro de Biologia Molecular Estrutural, Departamento de Bioquimica, Universidade Federal de Santa Catarina, 88040 900 Florianopolis, SC, Brazil
Biotechnol Appl Biochem 53:165-74. 2009....
Potyvirus genome-linked protein (VPg) determines pea seed-borne mosaic virus pathotype-specific virulence in Pisum sativumK E Keller
USDA ARS, Horticultural Crops Research Laboratory, Oregon State University, Corvallis 97330, USA
Mol Plant Microbe Interact 11:124-30. 1998The mechanism of Pisum sativum pathotype-specific resistance to pea seed-borne mosaic potyvirus (PSbMV) was investigated and the coding region determinant of PSbMV virulence was defined...
Simultaneous equimolar expression of multiple proteins in plants from a disarmed potyvirus vectorLeonor Bedoya
Instituto de Biologia Molecular y Celular de Plantas, Consejo Superior de Investigaciones Cientificas Universidad Politecnica de Valencia, Avenida de los Naranjos, 46022 Valencia, Spain
J Biotechnol 150:268-75. 2010..The technology is based on the replacement of the viral RNA polymerase (NIb) cistron of a potyvirus by a cassette for the coexpression of multiple heterologous proteins...
A novel recombinant strain of Potato virus Y suggests a new viral genetic determinant of vein necrosis in tobaccoXiaojun Hu
University of Idaho, Department of PSES, Moscow, ID 83844, United States
Virus Res 143:68-76. 2009..Taken together, these data suggest that the vein necrosis genetic determinant of PVY in tobacco is complex and includes other element(s), in addition to the C-terminal fragment of HC-Pro...
Engineering zucchini yellow mosaic potyvirus as a non-pathogenic vector for expression of heterologous proteins in cucurbitsT Arazi
ViroGene Limited, Har-Hotzvim, P.O. Box 45010, 91045, Jerusalem, Israel
J Biotechnol 87:67-82. 2001..A novel attenuated zucchini yellow mosaic potyvirus (AG) was used for the development of an environmentally safe non-pathogenic virus vector...
The I gene of bean: a dosage-dependent allele conferring extreme resistance, hypersensitive resistance, or spreading vascular necrosis in response to the potyvirus Bean common mosaic virusC W Collmer
Department of Biological and Chemical Sciences, Wells College, Aurora, NY 13026, USA
Mol Plant Microbe Interact 13:1266-70. 2000The resistance to the potyvirus Bean common mosaic virus (BCMV) conferred by the I allele in cultivars of Phaseolus vulgaris has been characterized as dominant, and it has been associated with both immunity and a systemic vascular ..
Kinetic mechanism for the binding of eIF4F and tobacco Etch virus internal ribosome entry site rna: effects of eIF4B and poly(A)-binding proteinMateen A Khan
Department of Chemistry and Biochemistry, Hunter College and Graduate Center of the City University of New York, New York, New York 10065, USA
J Biol Chem 284:35461-70. 2009..Poly(A)-binding protein and eIF4B mainly affect the eIF4F/TEV association rate. These results demonstrate the first direct kinetic measurements of translation initiation factor binding to an IRES...
From hypo- to hypersuppression: effect of amino acid substitutions on the RNA-silencing suppressor activity of the Tobacco etch potyvirus HC-ProClara Torres-Barceló
Instituto de Biologia Molecular y Celular de Plantas, Consejo Superior de Investigaciones Científicas UPV, 46022 Valencia, Spain
Genetics 180:1039-49. 2008..Tobacco etch potyvirus (TEV) HC-Pro protein suppresses RNA silencing and participates in aphid-mediated transmission, polyprotein ..
A new approach for the simultaneous differentiation of biological and geographical strains of Potato virus Y by uniplex and multiplex RT-PCRXianzhou Nie
Potato Research Centre, Agriculture and Agri-Food Canada, P.O. Box 20280, Fredericton, NB, Canada E3B 4Z7
J Virol Methods 104:41-54. 2002..Primer pairs can be used in a uniplex (single pair of primer) or multiplex (duplex, tetraplex or pentaplex) competitive RT-PCR, allowing simultaneous testing for any combination of PVY(O), EU-PVY(N/NTN), NA-PVY(N) and NA-PVY(NTN)...
Probable geographical grouping of PVY(N) and PVY(NTN) based on sequence variation in P1 and 5'-UTR of PVY genome and methods for differentiating North American PVY(NTN)Xianzhou Nie
Potato Research Centre, Agriculture and Agri-Food Canada, Fredericton, Canada
J Virol Methods 103:145-56. 2002..Thus sequencing of the P1 gene and use of competitive RT-PCR approach could be applicable for determining the possible origin of new occurrences of PVY(NTN) from other geographical regions...
Mapping tightly linked genes controlling potyvirus infection at the Rsv1 and Rpv1 region in soybeanM A Gore
Department of Crop and Soil Environmental Sciences, Virginia Polytechnic Institute and State University, Blacksburg 24061, USA
Genome 45:592-9. 2002..Isolating and characterizing Rsv1 and Rpv1 are instrumental in providing insight into the molecular mechanism of potyvirus recognition in soybean...
Processive degradation of nascent polypeptides, triggered by tandem AGA codons, limits the accumulation of recombinant tobacco etch virus protease in Escherichia coli BL21(DE3)Rachel B Kapust
Protein Engineering Section, National Cancer Institute at Frederick, Frederick, Maryland 21702-1201, USA
Protein Expr Purif 24:61-70. 2002....
Characterisation of a potyvirus and a potexvirus from Chinese scallionJ Che
Department of Biotechnology, College of Life Sciences, Zhejiang University, Hangzhou, People s Republic of China
Arch Virol 147:683-93. 2002..The complete sequences of a potyvirus and a potexvirus were determined and these were shown to represent different viruses from any in the databases...
Mutational analysis of plant cap-binding protein eIF4E reveals key amino acids involved in biochemical functions and potyvirus infectionSylvie German-Retana
Interactions Plante Virus, UMR GDPP 1090, INRA Université de Bordeaux 2, BP 81, F 33883 Villenave d Ornon Cedex, France
J Virol 82:7601-12. 2008..In addition to providing a functional mutational map of a plant eIF4E, this suggests that the role of eIF4E in the LMV cycle might be distinct from its physiological function in cellular mRNA translation...
Characterization of a new potyvirus infecting pepper crops in EcuadorBerenger Janzac
Institut National de la Recherche Agronomique, UR 407 Pathologie Végétale, domaine St Maurice BP 94, Montfavet, France
Arch Virol 153:1543-8. 2008Sequencing 2,951 nucleotides of the 3' proximal region of the genome of a potyvirus isolate collected from Capsicum pubescens (rocoto) pepper in Ecuador revealed that this was the first representative of a new species tentatively named ..
Potato virus A genome-linked protein VPg is an intrinsically disordered molten globule-like protein with a hydrophobic coreKimmo I Rantalainen
Department of Applied Chemistry and Microbiology, PO Box 27, FIN 00014, University of Helsinki, Finland
Virology 377:280-8. 2008Genome-linked protein VPg of Potato virus A (PVA; genus Potyvirus) has essential functions in all critical steps of PVA infection, i.e. replication, movement, and virulence...
Helper component proteinase of the genus Potyvirus is an interaction partner of translation initiation factors eIF(iso)4E and eIF4E and contains a 4E binding motifMarjo Ala-Poikela
Department of Agricultural Sciences, P O Box 27, FI 00014, University of Helsinki, Finland
J Virol 85:6784-94. 2011The multifunctional helper component proteinase (HCpro) of potyviruses (genus Potyvirus; Potyviridae) shows self-interaction and interacts with other potyviral and host plant proteins...
Double mutations in eIF4E and eIFiso4E confer recessive resistance to Chilli veinal mottle virus in pepperJeena Hwang
Department of Plant Science, Plant Genomics and Breeding Institute, and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 151 921, Korea
Mol Cells 27:329-36. 2009..These results demonstrated that ChiVMV can use both eIF4E and eIFiso4E for replication, making simultaneous mutations in eIF4E and eIFiso4E necessary to prevent ChiVMV infection in pepper...
Evidence for RNA-mediated defence effects on the accumulation of Potato leafroll virusH Barker
Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, UK
J Gen Virol 82:3099-106. 2001..by producing tobacco plants in which cells contained both PLRV cDNA and DNA encoding the P1/HC-Pro genes of the potyvirus Tobacco etch virus...
Characterization of a new potyvirus isolated from Chinese artichoke in JapanS Fuji
Faculty of Bioresource Sciences, Akita Prefectural University, Shimoshinjo, Akita, Japan
Arch Virol 148:2249-55. 2003..This virus was assigned to the genus Potyvirus, based on particle morphology and serological properties...
The determinant of potyvirus ability to overcome the RTM resistance of Arabidopsis thaliana maps to the N-terminal region of the coat proteinV Decroocq
UMR GDPP, INRA Université Bordeaux II, IBVM, Centre INRA de Bordeaux, BP 81, 33883 Villenave d Ornon Cedex France
Mol Plant Microbe Interact 22:1302-11. 2009..Taken together, these findings demonstrate that the potyvirus CP N-terminal region determines the outcome of the interaction with the RTM-mediated resistance.
Structural analysis of the eukaryotic initiation factor 4E gene controlling potyvirus resistance in pepper: exploitation of a BAC librarySandrine Ruffel
INRA, Plant Genomics Research Unit, 2 rue G. Cremieux, CP 5708, F-91057 Evry Cedex, France
Gene 338:209-16. 2004..a eukaryotic translation initiation factor 4E (eIF4E) gene that confers resistance to viruses belonging to the potyvirus genus...
Suppressor activity of potyviral and cucumoviral infections in potyvirus-induced transgene silencingCarmen Simón-Mateo
Centro Nacional de Biotecnología C S I C, Campus de la Universidad Autonoma de Madrid, 28049 Madrid, Spain
J Gen Virol 84:2877-83. 2003..Surprisingly, only the silencing suppression caused by CMV, but not that originating from TVMV, was able to revert the recovered NIbV3 plants to a PPV-susceptible phenotype...
Strain-specific interaction of the tobacco etch virus NIa protein with the translation initiation factor eIF4E in the yeast two-hybrid systemM C Schaad
Institute of Biological Chemistry, Washington State University, Pullman, Washington 99164 6340, USA
Virology 273:300-6. 2000..In TEV-eIF4E coexpression experiments, evidence suggesting that eIF4E provides a positive effect on genome amplification was obtained...
The eukaryotic translation initiation factor 4E controls lettuce susceptibility to the Potyvirus Lettuce mosaic virusValerie Nicaise
Interactions Plante Virus, Institut de Biologie Végétale Moléculaire, Institut National de la Recherche Agronomique, boite postale 81, F 33883 Villenave d Ornon Cedex, France
Plant Physiol 132:1272-82. 2003..Lactuca sativa) that are susceptible, tolerant, or resistant to infection by Lettuce mosaic virus (LMV; genus Potyvirus) were cloned and sequenced...
Comparison of the substrate specificity of two potyvirus proteasesJozsef Tozser
Department of Biochemistry and Molecular Biology, Research Center for Molecular Medicine, University of Debrecen, Debrecen, Hungary
FEBS J 272:514-23. 2005..Additional residues in the substrate-binding subsites of TEV protease were also mutated in an effort to alter the specificity of the enzyme...
Characterisation of an isolate of Narcissus degeneration virus from Chinese narcissus (Narcissus tazetta var. chinensis)J Chen
Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, PR China
Arch Virol 152:441-8. 2007A potyvirus from Chinese narcissus was transmitted mechanically to three species of Narcissus and to Lycoris radiata but not to 22 other test species...
Salicylic acid-mediated and RNA-silencing defense mechanisms cooperate in the restriction of systemic spread of plum pox virus in tobaccoJosefa M Alamillo
Department of Plant Molecular Genetics, , , 28049 Madrid, Spain
Plant J 48:217-27. 2006..Both an RNA-silencing and an SA-mediated defense mechanism could act together to limit PPV infection...
Three heterologous proteins simultaneously expressed from a chimeric potyvirus: infectivity, stability and the correlation of genome and virion lengthsJani Kelloniemi
Department of Applied Biology, P O Box 27, FIN 00014 University of Helsinki, Finland
Virus Res 135:282-91. 2008Three heterologous proteins were simultaneously expressed from a chimeric potyvirus Potato virus A (PVA) in Nicotiana benthamiana...
A new potyvirus from tuberose ( Polianthes tuberosa) in ChinaL Lin
Department of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, People s Republic of China
Arch Virol 149:1107-16. 2004..mottle symptoms, growing in a glasshouse in Hangzhou, China, contained virions and inclusion bodies typical of a potyvirus. The virus was mechanically transmitted to tuberose but not to 14 other test plant species...
The genomic sequence of Wisteria vein mosaic virus and its similarities with other potyvirusesW X Liang
Department of Plant Pathology and State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing, PR China
Arch Virol 151:2311-9. 2006..acid sequences support the current status of Wisteria vein mosaic virus (WVMV) as a distinct virus of the genus Potyvirus and a member of the Bean common mosaic virus (BCMV) subgroup...
Complete nucleotide sequence of the genomic RNA of Narcissus yellow stripe virus from Chinese narcissus in Zhangzhou city, ChinaJ Chen
Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, PR China
Arch Virol 151:1673-7. 2006
Rapid identification of potato virus Y strains by one-step triplex RT-PCRStefania Rigotti
Agroscope Changins Wädenswil Research Station ACW, Department of Virology, CH 1260 Nyon 1, Switzerland
J Virol Methods 140:90-4. 2007..The one-step triplex RT-PCR described here fastens specific detection of PVY strains that are otherwise only distinguishable by combined serological and biological assays...
Vanilla mosaic virus isolates from French Polynesia and the Cook Islands are Dasheen mosaic virus strains that exclusively infect vanillaK Farreyrol
School of Biological Sciences, The University of Auckland, Auckland, New Zealand
Arch Virol 151:905-19. 2006..In accordance with current ICTV criteria for species demarcation within the family Potyviridae, VanMV-CI and VanMV-FP are strains of DsMV that exclusively infect vanilla...
Whole genome characterization of Potato virus Y isolates collected in the western USA and their comparison to isolates from Europe and CanadaJ H Lorenzen
PSES Department, University of Idaho, Moscow, Idaho 83844, USA
Arch Virol 151:1055-74. 2006....
Cowpea aphid-borne mosaic virus (CABMV) is widespread in passionfruit in Brazil and causes passionfruit woodiness diseaseA V S Nascimento
Departamento de Fitopatologia/BIOAGRO, , , Brazil
Arch Virol 151:1797-809. 2006..Together, these results provide unequivocal evidence that, in Brazil, passionfruit woodiness disease is primarily caused by CABMV. The presence of PWV in Brazil has yet to be confirmed...
Konjak mosaic virus: the complete nucleotide sequence of the genomic RNA and its comparison with other potyvirusesM Nishiguchi
National Institute of Agrobiological Sciences, Tsukuba, Japan
Arch Virol 151:1643-50. 2006Konjak mosaic virus (KoMV) belongs to the genus Potyvirus, family Potyviridae. The complete nucleotide sequence of KoMV F isolate (KoMV F) was determined...
Molecular variability of the coat protein gene of Potato virus Y from tobacco in ChinaN Li
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing
Acta Virol 50:107-13. 2006..6%. The highest identity was observed in the C-terminal half of the CP protein, where only 11 amino acid differences could be observed, in contrast to the N-terminal half with 22 differences...
Molecular characterization of Czech and Chinese leek yellow stripe virus isolates from garlicM Navratil
Faculty of Sciences, Palack y University, 783 71 Olomouc, Czech Republic
Acta Virol 50:207-10. 2006..The RT-PCR products were cloned and sequenced. Phylogenetic analysis based on deduced amino acid sequence of NIb-CP region placed the Czech isolates in the group I and the Chinese isolate in the group II of LYSV...
Complete genome sequence of an isolate of Papaya ringspot virus from IndiaB Parameswari
Unit of Plant Virology, Division of Plant Pathology, Indian Agricultural Research Institute, New Delhi, India
Arch Virol 152:843-5. 2007
A new potyvirus from butterfly flower (Iris japonica Thunb.) in Zhejiang, ChinaJ Chen
Faculty of Life Science and Biotechnology, Ningbo University, Ningbo, 315211, Zhejiang Province, People s Republic of China
Arch Virol 153:567-9. 2008..Sequencing of the 3'-terminus of the genome showed that it was a potyvirus, most closely related to sunflower mosaic virus (63...
Genetic structure of a population of Potato virus Y inducing potato tuber necrotic ringspot disease in Japan; comparison with North American and European populationsTetsuji Ogawa
Aino Potato Branch, Nagasaki Agriculture and Forestry Experiment Station, Nagasaki 854 0302, Japan
Virus Res 131:199-212. 2008..Our results suggest that PVY PTNRD was recently introduced into Japan more than once, and has expanded throughout Japan from founder populations...
Biological characterization and complete nucleotide sequence of a Tunisian isolate of Moroccan watermelon mosaic virusS Yakoubi
Laboratoire de Genetique Moleculaire, Immunologie et Biotechnologie, Faculte des Sciences, Tunis, Tunisia
Arch Virol 153:117-25. 2008..DAS-ELISA showed the presence of Moroccan watermelon mosaic virus (MWMV, Potyvirus), detected for the first time in Tunisia, in samples from the region of Cap Bon (Northern Tunisia)...
Research Grants
- GENETIC ANALYSIS OF RNA VIRUS-HOST INTERACTIONJAMES CARRINGTON; Fiscal Year: 2007..model host to address the functions of two cellular compatibility factors that were identified in screens for potyvirus susceptibility mutants...
- EVOLUTION OF IMMUNE RECOGNITION AND EVASIONAustin Hughes; Fiscal Year: 2006..abstract_text> ..
- EVOLUTION OF IMMUNE RECOGNITION AND EVASIONAustin Hughes; Fiscal Year: 2007..In addition, by increasing our understanding of the population diversity and evolutionary history of human immune system genes, this research will enhance our knowledge of human immune function in health and disease. ..
- EVOLUTION OF IMMUNE RECOGNITION AND EVASIONAustin Hughes; Fiscal Year: 2009..In addition, by increasing our understanding of the population diversity and evolutionary history of human immune system genes, this research will enhance our knowledge of human immune function in health and disease. ..
- GENETICS OF ALZHEIMER'S DISEASE IN ISRAELI ARABSROBERT FRIEDLAND; Fiscal Year: 2009..The ultimate goal of this study is to find new targets (genetic and non-genetic) for therapy. ..
- Symposium on Alzheimer's Disease in the Middle EastROBERT FRIEDLAND; Fiscal Year: 2005..Topics to be covered include dementia, basic science, diagnoses, history, management and treatment, and ethics, with consideration of the unique cultural traditions of Arab and Jewish populations. ..
- Cong Intl. Soc Vascular Behavioral & Cognitive DisordersROBERT FRIEDLAND; Fiscal Year: 2005..Our second event will present in one large Congress the most recent advances in the field, including diagnostic and therapeutic developments by internationally recognized opinion leaders. ..
- EVOLUTION OF IMMUNE RECOGNITION AND EVASIONAustin Hughes; Fiscal Year: 2002....
- Symposium on Alzheimer's Disease in the Middle EastROBERT FRIEDLAND; Fiscal Year: 2003..Topics to be covered include dementia, basic science, diagnoses, history, management and treatment, and ethics, with consideration of the unique cultural traditions of Arab and Jewish populations. ..
- International Society for Vascular Behavioral DisordersROBERT FRIEDLAND; Fiscal Year: 2003..This meeting will present in one large Congress the most recent advances in the field, including diagnostic and therapeutic developments by internationally recognized opinion leaders. ..
- Gene Duplication and Genome EvolutionAustin Hughes; Fiscal Year: 2004..5) Examining the role of transposable elements in duplication of genomic segments by testing for nonrandom association between these elements and putatively duplicated blocks in the yeast genome. ..
- EVOLUTION OF IMMUNE RECOGNITION AND EVASIONAustin L Hughes; Fiscal Year: 2010..In addition, by increasing our understanding of the population diversity and evolutionary history of human immune system genes, this research will enhance our knowledge of human immune function in health and disease. ..
