bromovirus

Summary

Summary: A genus of tripartite plant viruses in the family BROMOVIRIDAE. Transmission is by beetles. Brome mosaic virus is the type species.

Top Publications

  1. pmc Interactions between brome mosaic virus RNAs and cytoplasmic processing bodies
    Carla J Beckham
    Department of Molecular and Cellular Biology and Howard Hughes Medical Institute, The University of Arizona, Tucson, AZ 85721 0206, USA
    J Virol 81:9759-68. 2007
  2. pmc RNA binding by the brome mosaic virus capsid protein and the regulation of viral RNA accumulation
    Guanghui Yi
    Department of Biology, Indiana University, Bloomington, IN 47405, USA
    J Mol Biol 391:314-26. 2009
  3. pmc Systematic, genome-wide identification of host genes affecting replication of a positive-strand RNA virus
    David B Kushner
    Institute for Molecular Virology and Howard Hughes Medical Institute, University of Wisconsin, Madison, WI 53706, USA
    Proc Natl Acad Sci U S A 100:15764-9. 2003
  4. pmc Role of surface charge density in nanoparticle-templated assembly of bromovirus protein cages
    Marie Christine Daniel
    Department of Chemistry, Indiana University, Bloomington, Indiana 47405, USA
    ACS Nano 4:3853-60. 2010
  5. ncbi A positive-strand RNA virus replication complex parallels form and function of retrovirus capsids
    Michael Schwartz
    Institute for Molecular Virology and Howard Hughes Medical Institute, University of Wisconsin, Madison, Madison, WI 53706, USA
    Mol Cell 9:505-14. 2002
  6. ncbi Mechanism of capsid assembly for an icosahedral plant virus
    A Zlotnick
    Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73190, USA
    Virology 277:450-6. 2000
  7. pmc cis-acting elements required for efficient packaging of brome mosaic virus RNA3 in barley protoplasts
    Tri Asmira Damayanti
    Laboratory of Plant Pathology, Graduate School of Agriculture, Kyoto University, Kyoto 606 8502, Japan
    J Virol 77:9979-86. 2003
  8. pmc Intersection of the multivesicular body pathway and lipid homeostasis in RNA replication by a positive-strand RNA virus
    Xiaofeng Wang
    Institute for Molecular Virology, University of Wisconsin, Madison, WI 53706, USA
    J Virol 85:5494-503. 2011
  9. pmc tRNA elements mediate the assembly of an icosahedral RNA virus
    Yoon Gi Choi
    Department of Plant Pathology, University of California, Riverside, CA, 92521 0122, USA
    Proc Natl Acad Sci U S A 99:655-60. 2002
  10. pmc RNA recombination in brome mosaic virus: effects of strand-specific stem-loop inserts
    R C L Olsthoorn
    Plant Molecular Biology Center, Department of Biological Sciences, Northern Illinois University, DeKalb, IL 60115 2861, USA
    J Virol 76:12654-62. 2002

Scientific Experts

Detail Information

Publications133 found, 100 shown here

  1. pmc Interactions between brome mosaic virus RNAs and cytoplasmic processing bodies
    Carla J Beckham
    Department of Molecular and Cellular Biology and Howard Hughes Medical Institute, The University of Arizona, Tucson, AZ 85721 0206, USA
    J Virol 81:9759-68. 2007
    ..These observations suggest that the accumulation of BMV RNAs in P bodies may be an important step in RNA replication complex assembly for BMV, and possibly for other positive-strand RNA viruses...
  2. pmc RNA binding by the brome mosaic virus capsid protein and the regulation of viral RNA accumulation
    Guanghui Yi
    Department of Biology, Indiana University, Bloomington, IN 47405, USA
    J Mol Biol 391:314-26. 2009
    ..These results better define how the BMV CP can interact with RNA and regulate different viral processes...
  3. pmc Systematic, genome-wide identification of host genes affecting replication of a positive-strand RNA virus
    David B Kushner
    Institute for Molecular Virology and Howard Hughes Medical Institute, University of Wisconsin, Madison, WI 53706, USA
    Proc Natl Acad Sci U S A 100:15764-9. 2003
    ..The results further illuminate virus and cell pathways. Further refinement of virus screening likely will reveal contributions from additional host genes...
  4. pmc Role of surface charge density in nanoparticle-templated assembly of bromovirus protein cages
    Marie Christine Daniel
    Department of Chemistry, Indiana University, Bloomington, Indiana 47405, USA
    ACS Nano 4:3853-60. 2010
    ..The current work furnishes evidence of a critical surface charge density for assembly through an analysis aimed at decoupling the surface charge and the core size...
  5. ncbi A positive-strand RNA virus replication complex parallels form and function of retrovirus capsids
    Michael Schwartz
    Institute for Molecular Virology and Howard Hughes Medical Institute, University of Wisconsin, Madison, Madison, WI 53706, USA
    Mol Cell 9:505-14. 2002
    ....
  6. ncbi Mechanism of capsid assembly for an icosahedral plant virus
    A Zlotnick
    Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73190, USA
    Virology 277:450-6. 2000
    ....
  7. pmc cis-acting elements required for efficient packaging of brome mosaic virus RNA3 in barley protoplasts
    Tri Asmira Damayanti
    Laboratory of Plant Pathology, Graduate School of Agriculture, Kyoto University, Kyoto 606 8502, Japan
    J Virol 77:9979-86. 2003
    ..Furthermore, the efficient packaging of RNA4 without RNA3 in B3Cmp-infected cells implies the presence of an element in the 3a ORF of BMV RNA3 that regulates the copackaging of RNA3 and RNA4...
  8. pmc Intersection of the multivesicular body pathway and lipid homeostasis in RNA replication by a positive-strand RNA virus
    Xiaofeng Wang
    Institute for Molecular Virology, University of Wisconsin, Madison, WI 53706, USA
    J Virol 85:5494-503. 2011
    ..Among other points, these findings show the potential for blocking viral RNA replication by modulating lipid synthesis at multiple levels...
  9. pmc tRNA elements mediate the assembly of an icosahedral RNA virus
    Yoon Gi Choi
    Department of Plant Pathology, University of California, Riverside, CA, 92521 0122, USA
    Proc Natl Acad Sci U S A 99:655-60. 2002
    ..We discuss the likely roles of the TLS in early stages of virion assembly...
  10. pmc RNA recombination in brome mosaic virus: effects of strand-specific stem-loop inserts
    R C L Olsthoorn
    Plant Molecular Biology Center, Department of Biological Sciences, Northern Illinois University, DeKalb, IL 60115 2861, USA
    J Virol 76:12654-62. 2002
    A model system of a single-stranded trisegment Brome mosaic bromovirus (BMV) was used to analyze the mechanism of homologous RNA recombination...
  11. ncbi Effects of the cowpea chlorotic mottle bromovirus beta-hexamer structure on virion assembly
    D Willits
    Department of Plant Sciences Plant Pathology, Montana State University, Bozeman, MT 59717, USA
    Virology 306:280-8. 2003
    The X-ray crystal structure of Cowpea chlorotic mottle bromovirus (CCMV) revealed a unique tubular structure formed by the interaction of the N-termini from six coat protein subunits at each three-fold axis of the assembled virion...
  12. pmc Enhanced local symmetry interactions globally stabilize a mutant virus capsid that maintains infectivity and capsid dynamics
    Jeffrey A Speir
    Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Rd, La Jolla, CA 92037, USA
    J Virol 80:3582-91. 2006
    ..e., fivefold, twofold, quasi-threefold axes). The result is a particle that is still dynamic but insensitive to high salt due to a new series of bonds that are resistant to high ionic strength and preserve the overall particle structure...
  13. pmc Bromovirus RNA replication compartment formation requires concerted action of 1a's self-interacting RNA capping and helicase domains
    Arturo Diaz
    Institute for Molecular Virologya and Howard Hughes Medical Institute, Madison, Madison, Wisconsin 53706, USA
    J Virol 86:821-34. 2012
    ..These and other findings shed light on the roles and interactions of 1a domains in replication compartment formation and support prior results suggesting that 1a induces replication vesicles by forming a capsid-like interior shell...
  14. ncbi Rapid and efficient purification of Cowpea chlorotic mottle virus by sucrose cushion ultracentrifugation
    Akhtar Ali
    Plant Biology Division, The Samuel Roberts Noble Foundation, Ardmore, OK 73401, USA
    J Virol Methods 141:84-6. 2007
    ..RNA extracted from purified CCMV particles showed all four RNA components by agarose gel electrophoresis. Both purified virus particles and RNA were biologically active and initiated successful infection upon inoculation to cowpea...
  15. pmc Systematic identification of novel, essential host genes affecting bromovirus RNA replication
    Brandi L Gancarz
    Institute for Molecular Virology, University of Wisconsin Madison, Madison, Wisconsin, United States of America
    PLoS ONE 6:e23988. 2011
    ..Investigating the diverse functions of these newly identified essential host genes should advance our understanding of BMV-host interactions and normal cellular pathways, and suggest new modes of virus control...
  16. pmc An amphipathic alpha-helix controls multiple roles of brome mosaic virus protein 1a in RNA replication complex assembly and function
    Ling Liu
    Institute for Molecular Virology, University of Wisconsin Madison, Madison, Wisconsin, USA
    PLoS Pathog 5:e1000351. 2009
    ....
  17. pmc Depurination within the intergenic region of Brome mosaic virus RNA3 inhibits viral replication in vitro and in vivo
    Rajita A Karran
    Department of Biology, York University, Toronto, Ontario, Canada
    Nucleic Acids Res 36:7230-9. 2008
    ..These results support a role of the intergenic region in controlling negative-strand RNA synthesis and contribute new insight into the effect of depurination by PAP on BMV replication...
  18. pmc Mutual interference between genomic RNA replication and subgenomic mRNA transcription in brome mosaic virus
    Valery Z Grdzelishvili
    Institute for Molecular Virology, University of Wisconsin Madison, 1525 Linden Dr, Madison, WI 53706 1596, USA
    J Virol 79:1438-51. 2005
    ....
  19. pmc Alternate, virus-induced membrane rearrangements support positive-strand RNA virus genome replication
    Michael Schwartz
    Institute for Molecular Virology and Howard Hughes Medical Institute, University of Wisconsin, Madison, WI 53706, USA
    Proc Natl Acad Sci U S A 101:11263-8. 2004
    ....
  20. pmc Packaging of brome mosaic virus RNA3 is mediated through a bipartite signal
    Yoon Gi Choi
    Department of Plant Pathology, University of California, Riverside, CA 92521 0122, USA
    J Virol 77:9750-7. 2003
    ....
  21. ncbi Pokeweed antiviral protein inhibits brome mosaic virus replication in plant cells
    Daniel Picard
    Department of Biology, York University, Toronto, Ontario, Canada
    J Biol Chem 280:20069-75. 2005
    ..These novel activities are distinct from the PAP-induced reduction of viral RNA translation and represent new targets for the inhibition of viral infection...
  22. pmc Brome mosaic virus 1a nucleoside triphosphatase/helicase domain plays crucial roles in recruiting RNA replication templates
    Xiaofeng Wang
    Institute for Molecular Virology, University of Wisconsin Madison, 1525 Linden Dr, Madison, WI 53706 1576, USA
    J Virol 79:13747-58. 2005
    ..The possibility that, similar to some double-stranded RNA viruses, the 1a NTPase/helicase-like domain may be involved in importing viral RNAs into a preformed replication compartment is discussed...
  23. pmc Homologous crossovers among molecules of brome mosaic bromovirus RNA1 or RNA2 segments in vivo
    Anna Urbanowicz
    Department of Biological Sciences, Montgomery Hall, Northern Illinois University, De Kalb, IL 60015, USA
    J Virol 79:5732-42. 2005
    ..demonstrated frequent homologous crossovers among molecules of the RNA3 segment in the tripartite brome mosaic bromovirus (BMV) RNA genome (A. Bruyere, M. Wantroba, S. Flasinski, A. Dzianott, and J. J. Bujarski, J. Virol...
  24. ncbi Replication-independent expression of genome components and capsid protein of brome mosaic virus in planta: a functional role for viral replicase in RNA packaging
    Padmanaban Annamalai
    Department of Plant Pathology, University of California, Riverside, CA 92521 0122, USA
    Virology 338:96-111. 2005
    ..These results demonstrated that packaging of BMV genomic RNAs is not replication dependent whereas expression of a functional viral replicase plays an active role in increasing specificity of RNA packaging...
  25. pmc RNA-binding proteins that inhibit RNA virus infection
    Jian Zhu
    Department of Pharmacology and Molecular Sciences and High Throughput Biology Center, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 104:3129-34. 2007
    ..Furthermore, the effects of Pus4 suggest that the CAM-containing RNA motif provides a regulatory link between RNA replication and encapsidation...
  26. pmc Mutation of host delta9 fatty acid desaturase inhibits brome mosaic virus RNA replication between template recognition and RNA synthesis
    W M Lee
    Howard Hughes Medical Institute, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA
    J Virol 75:2097-106. 2001
    ..The strong unsaturated fatty acid dependence also demonstrates that modulating fatty acid balance can be an effective antiviral strategy...
  27. pmc An alternate pathway for recruiting template RNA to the brome mosaic virus RNA replication complex
    Jianbo Chen
    Institute for Molecular Virology, University of Wisconsin, 1525 Linden Drive, Madison, WI 53706 1596, USA
    J Virol 77:2568-77. 2003
    ..Interaction with nascent 2a also may be involved in 1a recruitment of 2a polymerase to membranes...
  28. pmc The AU-rich RNA recombination hot spot sequence of Brome mosaic virus is functional in tombusviruses: implications for the mechanism of RNA recombination
    Natalia Shapka
    Department of Plant Pathology, University of Kentucky, Lexington, Kentucky 40546, USA
    J Virol 78:2288-300. 2004
    ..In summary, evidence is presented that a tombusvirus can use the recombination signal of BMV. This supports the idea that common AU-rich recombination signals might promote interviral recombination between unrelated viruses...
  29. pmc Identification of sequences in Brome mosaic virus replicase protein 1a that mediate association with endoplasmic reticulum membranes
    J A den Boon
    Institute for Molecular Virology, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA
    J Virol 75:12370-81. 2001
    ....
  30. pmc Efficient in vitro system of homologous recombination in brome mosaic bromovirus
    Rafal Wierzchoslawski
    Plant Molecular Biology Center, Department of Biological Sciences, Northern Illinois University, De Kalb, IL 60115, USA
    J Virol 80:6182-7. 2006
    Recent in vivo studies have revealed that the subgenomic promoter (sgp) in brome mosaic bromovirus (BMV) RNA3 supports frequent homologous recombination events (R. Wierzchoslawski, A. Dzianott, and J. Bujarski, J. Virol...
  31. pmc The brome mosaic virus RNA3 intergenic replication enhancer folds to mimic a tRNA TpsiC-stem loop and is modified in vivo
    T Baumstark
    Institute for Molecular Virology and Howard Hughes Medical Institute, University of Wisconsin Madison, 53706, USA
    RNA 7:1652-70. 2001
    ....
  32. pmc Replicase-binding sites on plus- and minus-strand brome mosaic virus RNAs and their roles in RNA replication in plant cells
    S K Choi
    Department of Biochemistry and Biophysics, Texas A and M University, Mail Stop 2128, College Station, TX 77843, USA
    J Virol 78:13420-9. 2004
    ..These results identify the replicase-binding sites in all of the BMV RNAs and suggest that the recognition of RNA3 is different from that of RNA1 and RNA2...
  33. ncbi Use of a defective RNA of broad bean mottle bromovirus for stable gene expression in legumes
    C Sandoval
    Departamento de Proteccion Vegetal, INIA, Carretera de la Coruña km 7 0, 28040 Madrid, Spain
    Arch Virol 153:1755-8. 2008
    A viral vector that expressed foreign genes was engineered using a cDNA clone of broad bean mottle bromovirus (BBMV) defective interfering (DI) RNA...
  34. pmc Stable RNA structures can repress RNA synthesis in vitro by the brome mosaic virus replicase
    Xin Zhang
    Department of Biology, Indiana University, Bloomington, Indiana 47405, USA
    RNA 9:555-65. 2003
    ..The results characterize another potential mechanism whereby the BMV replicase can specifically recognize BMV RNAs...
  35. pmc Mutation of host DnaJ homolog inhibits brome mosaic virus negative-strand RNA synthesis
    Yuriko Tomita
    Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Sapporo 060 8589, Japan
    J Virol 77:2990-7. 2003
    ....
  36. ncbi Heterologous expression of the modified coat protein of Cowpea chlorotic mottle bromovirus results in the assembly of protein cages with altered architectures and function
    Susan Brumfield
    Department of Plant Sciences and Plant Pathology, Montana State University, Bozeman, MT 59717, USA
    J Gen Virol 85:1049-53. 2004
    ..Expression of the structural protein of Cowpea chlorotic mottle bromovirus (CCMV) using the yeast-based Pichia pastoris heterologous expression system resulted in the assembly of ..
  37. pmc Host deadenylation-dependent mRNA decapping factors are required for a key step in brome mosaic virus RNA replication
    Antonio Mas
    Departamento de Ciencias Experimentales y de la Salud, Universitat Pompeu Fabra, Dr Aiguader 80, 08003 Barcelona, Spain
    J Virol 80:246-51. 2006
    ..Collectively, our results suggest that the Lsm1p-7p/Pat1p/Dhh1p complex that transfers cellular mRNAs from translation to degradation might act as a key regulator in the switch from BMV RNA translation to replication...
  38. ncbi The role of subunit hinges and molecular "switches" in the control of viral capsid polymorphism
    Jinghua Tang
    Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    J Struct Biol 154:59-67. 2006
    ..These results show that capsid geometry is flexible and may readily adapt to new requirements as the virus evolves...
  39. pmc Membrane synthesis, specific lipid requirements, and localized lipid composition changes associated with a positive-strand RNA virus RNA replication protein
    Wai Ming Lee
    Howard Hughes Medical Institute and Institute for Molecular Virology, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    J Virol 77:12819-28. 2003
    ..This localized UFA depletion helps to explain why BMV RNA replication is more sensitive than cell growth to reduced UFA levels. The results imply that 1a preferentially interacts with one or more types of membrane lipids...
  40. pmc Brome mosaic virus Protein 1a recruits viral RNA2 to RNA replication through a 5' proximal RNA2 signal
    J Chen
    Institute for Molecular Virology, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA
    J Virol 75:3207-19. 2001
    ..These results support and expand the conclusion that 1a-induced BMV RNA stabilization and membrane association reflect early, 1a-mediated steps in viral RNA replication...
  41. pmc Yeast Lsm1p-7p/Pat1p deadenylation-dependent mRNA-decapping factors are required for brome mosaic virus genomic RNA translation
    Amine O Noueiry
    Institute for Molecular Virology Howard Hughes Medical Institute, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    Mol Cell Biol 23:4094-106. 2003
    ....
  42. pmc RNA motifs mediating in vivo site-specific nonhomologous recombination in (+) RNA virus enforce in vitro nonhomologous crossovers with HIV-1 reverse transcriptase
    M Figlerowicz
    Institute of Bioorganic Chemistry Polish Academy of Sciences, Poznan
    RNA 6:339-51. 2000
    ..These results indicate that HIV-RT utilizes the same or a very similar mechanism as BMV replicase to change nonhomologous RNA templates in a site-specific manner...
  43. pmc A conserved hairpin structure in Alfamovirus and Bromovirus subgenomic promoters is required for efficient RNA synthesis in vitro
    P C Haasnoot
    Institute of Molecular Plant Sciences, Gorlaeus Laboratories, Leiden University, The Netherlands
    RNA 6:708-16. 2000
    ..Using RNA templates corresponding to the sgp of brome mosaic virus (BMV; genus Bromovirus, family Bromoviridae) and purified BMV RdRp, evidence was obtained indicating that also in BMV RNA a triloop ..
  44. pmc Role of RNA structure in non-homologous recombination between genomic molecules of brome mosaic virus
    M Figlerowicz
    Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12 14, 61 704 Pozna, Poland
    Nucleic Acids Res 28:1714-23. 2000
    ..Moreover, it was shown that both short homologous sequences and a hairpin structure have to accompany a double-stranded region to target non-homologous crossovers in a site-specific manner...
  45. ncbi Synthesis of infectious in vitro transcripts from Cassia yellow blotch bromovirus cDNA clones and a reassortment analysis with other bromoviruses in protoplasts
    F Iwahashi
    Laboratory of Plant Pathology, Graduate School of Agriculture, Kyoto University, Kyoto, Japan
    Arch Virol 150:1301-14. 2005
    Cassia yellow blotch virus (CYBV), genus Bromovirus, was isolated from the Australian native legume, Cassia pleurocarpa, in western Queensland, and its host range was found to be distinct from other bromoviruses...
  46. ncbi Phosphorylation and interaction of the movement and coat proteins of brome mosaic virus in infected barley protoplasts
    N Akamatsu
    Laboratory of Plant Pathology, Graduate School of Agriculture, Kyoto University, Kyoto, Japan
    Arch Virol 152:2087-93. 2007
    ..Both BCP and B3a were detected in a complex immunoprecipitated from BMV-infected protoplasts with anti-B3a antiserum, implying an interaction between BCP and B3a...
  47. pmc Deletion of highly conserved arginine-rich RNA binding motif in cowpea chlorotic mottle virus capsid protein results in virion structural alterations and RNA packaging constraints
    Padmanaban Annamalai
    Department of Plant Pathology, University of California, 3264A Webber Hall, Riverside, CA 92521 0122, USA
    J Virol 79:3277-88. 2005
    ..Northern blot analysis of RNA encapsidation in vivo of two distinct bromovirus RNA3 chimeras, constructed by exchanging CPs having the Delta919 deletion, demonstrated that the role of the ..
  48. ncbi The disassembly, reassembly and stability of CCMV protein capsids
    Laurence Lavelle
    Department of Chemistry and Biochemistry, University of California Los Angeles, Los Angeles, CA 90095, USA lavelle chem ucla edu
    J Virol Methods 146:311-6. 2007
    ..It was also found that CaCl(2)/RNA precipitation methods do not yield sufficiently pure protein samples...
  49. pmc Replication-coupled packaging mechanism in positive-strand RNA viruses: synchronized coexpression of functional multigenome RNA components of an animal and a plant virus in Nicotiana benthamiana cells by agroinfiltration
    Padmanaban Annamalai
    University of California, Plant Pathology, 3264 Webber Hall, Riverside, CA 92521 0122, USA
    J Virol 82:1484-95. 2008
    ..Taken together, we propose that the transcription of CP mRNA from homologous replication and its translation must be synchronized to confer packaging specificity...
  50. doi Biochemical analyses of the interactions between viral polymerases and RNAs
    Young Chan Kim
    Department of Biochemistry and Biophysics, 103 Biochemistry Biophysics Building, Texas A and M University, 2128 TAMU, College Station, TX 77843 2128, USA
    Methods Mol Biol 451:185-200. 2008
    ..These analyses of RdRp-RNA interaction will be presented as three topics in this chapter...
  51. pmc Packaging of brome mosaic virus subgenomic RNA is functionally coupled to replication-dependent transcription and translation of coat protein
    Padmanaban Annamalai
    Department of Plant Pathology, University of California, Riverside, CA 92521 0122, USA
    J Virol 80:10096-108. 2006
    ....
  52. ncbi Two types of non-homologous RNA recombination in brome mosaic virus
    Magdalena Alejska
    Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznan, Poland
    Acta Biochim Pol 52:833-44. 2005
    ..There are, however, several observations questioning this model and indicating that some other factors, which are still unknown, may influence heteroduplex-mediated crossovers...
  53. pmc Inducible yeast system for Viral RNA recombination reveals requirement for an RNA replication signal on both parental RNAs
    Hernan Garcia-Ruiz
    Institute for Molecular Virology and Department of Plant Pathology, University of Wisconsin Madison, 53706 USA
    J Virol 80:8316-28. 2006
    ....
  54. pmc Nanoindentation studies of full and empty viral capsids and the effects of capsid protein mutations on elasticity and strength
    J P Michel
    Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095 1569, USA
    Proc Natl Acad Sci U S A 103:6184-9. 2006
    ..These calculations capture the features of the reversible indentation region and allow Young's moduli and relative strengths to be estimated for the empty capsids...
  55. doi RNA encapsidation assay
    Padmanaban Annamalai
    Department of Plant Pathology, University of California, Riverside, California, CA 92521 0122, USA
    Methods Mol Biol 451:251-64. 2008
    ..encapsidation. Northern blots are normally hybridized with radioactively labeled RNA probes (riboprobes) for specific and sensitive detection of desired RNA species...
  56. pmc In vivo particle polymorphism results from deletion of a N-terminal peptide molecular switch in brome mosaic virus capsid protein
    Shauni L Calhoun
    Department of Plant Pathology, University of California, Riverside, CA 92521, USA
    Virology 364:407-21. 2007
    ..Based on these observations, we propose a chaperon-mediated mechanism for the assembly of variant CP in vivo and also hypothesize that (41)KAIKAIA(47) N-proximal peptide functions as a molecular switch in regulating T=3 virion symmetry...
  57. pmc cis- and trans-acting functions of brome mosaic virus protein 1a in genomic RNA1 replication
    Guanghui Yi
    Department of Biochemistry and Biophysics, Texas A and M University, Mail Stop 2128, College Station, TX 77843, USA
    J Virol 82:3045-53. 2008
    ..Schwartz, J. Chen, M. Janda, M. Sullivan, J. den Boon, and P. Ahlquist, Mol. Cell 9:505-514, 2002). Results in this work demonstrate a linkage between BMV RNA1 translation and replication...
  58. ncbi Sensitivity of brome mosaic virus RNA1 replication to mutations in the 3' tRNA-like structure implies a requirement for sustained synthesis of replicase protein 1a
    A L N Rao
    Department of Plant Pathology, University of California, Riverside, California 92521 0122, USA
    Arch Virol 151:721-33. 2006
    ..Taken together the results suggest that, unlike protein 2a which is required in catalytic amounts, the intrinsic involvement of protein 1a at various stages of virus infection cycle demands its sustained synthesis...
  59. pmc Electrostatic properties of cowpea chlorotic mottle virus and cucumber mosaic virus capsids
    Robert Konecny
    Department of Chemistry and Biochemistry, University of California at San Diego, La Jolla, 92093 0365, USA
    Biopolymers 82:106-20. 2006
    ..Favorable binding to the swollen form suggests that Ca(2+) ions can induce the capsid contraction and stabilize the native form...
  60. pmc Interaction between Brome mosaic virus proteins and RNAs: effects on RNA replication, protein expression, and RNA stability
    K Gopinath
    Department of Biochemistry and Biophysics, Texas A and M University, College Station, TX 77843, USA
    J Virol 79:14222-34. 2005
    ....
  61. pmc A transcriptionally active subgenomic promoter supports homologous crossovers in a plus-strand RNA virus
    Rafal Wierzchoslawski
    Plant Molecular Biology Center, Department of Biological Sciences, Northern Illinois University, De Kalb, Illinois 60115, USA
    J Virol 77:6769-76. 2003
    ..activity that is supported by a subgenomic promoter (sgp) region in the RNA3 segment of brome mosaic bromovirus (BMV), a tripartite plus-strand RNA virus...
  62. pmc Repair of the tRNA-like CCA sequence in a multipartite positive-strand RNA virus
    M Hema
    Department of Biochemistry and Biophysics, Texas A and M University, College Station, TX 77843, USA
    J Virol 79:1417-27. 2005
    ..Instead, the repaired ends were heterogeneous, with one-third having a restored CCA and others having sequences with the only commonality being the restoration of one cytidylate. The role of BMV RNA1 in increased repair was examined...
  63. pmc Template sequence near the initiation nucleotide can modulate brome mosaic virus RNA accumulation in plant protoplasts
    M Hema
    Department of Biophysics and Biochemistry, Texas A and M University, College Station, Texas 77843, USA
    J Virol 78:1169-80. 2004
    ..These findings demonstrate that there are different requirements of the template sequence near the initiation nucleotide for BMV RNA accumulation in plant cells...
  64. ncbi Can the RNA of the cowpea chlorotic mottle virus be released through a channel by means of free diffusion? A test in silico
    Raul Isea
    CeCalCULA, Centro Nacional de Cálculo Científico Universidad de Los Andes, Edif Masini, Av 4, Calles 18 y 19, Piso 3, Ofic B32, Merida 5101, Venezuela
    Biophys Chem 107:101-6. 2004
    ..These results are congruent with the hypothesis suggesting that the CCMV capside does not have to dissolve in order to release the RNA into the host...
  65. ncbi Infection and RNA recombination of Brome mosaic virus in Arabidopsis thaliana
    Aleksandra Dzianott
    Department of Biological Sciences, Plant Molecular Biology Center, Northern Illinois University, DeKalb, IL 60115, USA
    Virology 318:482-92. 2004
    ..The current scope of understanding of the model Arabidopsis plant system should facilitate the identification of these factors governing the BMV life cycle...
  66. ncbi Effects of artificial codon changes in the movement protein gene on adaptation of a hybrid bromovirus to cowpea
    Nobumitsu Sasaki
    Laboratory of Plant Pathology, Graduate School of Agriculture, Kyoto University
    Microbiol Immunol 48:131-5. 2004
    ..We discuss mechanisms that restrict the occurrence of cowpea-adapted mutants in nature...
  67. ncbi Brome mosaic virus replicase proteins localize with the movement protein at infection-specific cytoplasmic inclusions in infected barley leaf cells
    K Dohi
    Laboratory of Plant Pathology, Graduate School of Agriculture, Kyoto University, Japan
    Arch Virol 146:1607-15. 2001
    ..The inclusions were oval or amorphous, and contained electron-lucent regions. Electron microscopic autoradiography showed that the inclusions were sites of [3H]uridine incorporation, suggesting that they are sites of viral RNA synthesis...
  68. ncbi Stop codon decoding in Candida albicans: from non-standard back to standard
    Gabriela Moura
    Centre for Cell Biology, Department of Biology, University of Aveiro, 3810 193 Aveiro, Portugal
    Yeast 19:727-33. 2002
    ..The data therefore show that the only aberrant event mediated by the ser-tRNA(CAG) is decoding of the leu-CUG codon as serine...
  69. ncbi The crystallographic structure of brome mosaic virus
    Robert W Lucas
    University of California Irvine, 560 Steinhaus Hall, Irvine, CA 92697 3900, USA
    J Mol Biol 317:95-108. 2002
    ..The density cannot be attributed to cations and it was modeled as a PEG molecule...
  70. ncbi The nucleotide sequence of hydrangea mosaic virus RNA 3 exhibits similarity with the RNA 3 of tobacco streak virus
    X Ge
    Department of Plant Pathology and Physiology, Clemson University, SC 29634 0377, USA
    Virus Res 40:57-63. 1996
    ..On the basis of the similarities between HdMV and TSV that we detected, we propose that HdMV be included in subgroup 1 of the genus Ilarvirus together with TSV...
  71. pmc Requirements for brome mosaic virus subgenomic RNA synthesis in vivo and replicase-core promoter interactions in vitro
    K Sivakumaran
    Texas A and M University, Department of Biochemistry and Biophysics, College Station, TX 77843, USA
    J Virol 78:6091-101. 2004
    ....
  72. ncbi The C terminus of the movement protein of Brome mosaic virus controls the requirement for coat protein in cell-to-cell movement and plays a role in long-distance movement
    Atsushi Takeda
    Laboratory of Plant Pathology, Graduate School of Agriculture, Kyoto University, Kyoto 606 8502, Japan
    J Gen Virol 85:1751-61. 2004
    ..Furthermore, the ability to spread locally and form virions is not sufficient for the long-distance movement of BMV. The roles of MP and CP in BMV movement are discussed...
  73. ncbi Structural and thermodynamic studies on mutant RNA motifs that impair the specificity between a viral replicase and its promoter
    C H Kim
    Department of Chemistry, University of California Berkeley, CA, 94720-1460, USA
    J Mol Biol 307:827-39. 2001
    ..Based on this study, we conclude that the key structural motif in the triloop recognized by the replicase is a solution-exposed, 5'-adenine base in the triloop that is clamped to the stem helix, which is called a clamped adenine motif...
  74. pmc Brome mosaic virus RNA syntheses in vitro and in barley protoplasts
    K Sivakumaran
    Department of Biology, Indiana University, Bloomington, Indiana 47405, USA
    J Virol 77:5703-11. 2003
    ....
  75. ncbi Structural analysis of a necrogenic strain of cucumber mosaic cucumovirus satellite RNA in planta
    G Rodriguez-Alvarado
    The S R Noble Foundation, Inc, Ardmore, Oklahoma, 73402, USA
    Virology 236:155-66. 1997
    ..in predicting structural models for some plant viruses such as tobacco mosaic tobamovirus and brome mosaic bromovirus. However, in planta conditions may be dramatically different from those found in vitro...
  76. doi PCR assays for the detection of members of the genus Ilarvirus and family Bromoviridae
    Milton Untiveros
    Plant Health and Environment Laboratory, Investigation and Diagnostic Centre, MAF Biosecurity New Zealand, PO Box 2095, Auckland 1140, New Zealand
    J Virol Methods 165:97-104. 2010
    ..to detect all species tested within the family Bromoviridae, including members of the Alfamovirus, Anulavirus, Bromovirus, Cucumovirus and Ilarvirus...
  77. ncbi The promiscuous evolutionary history of the family Bromoviridae
    Francisco M Codoñer
    Instituto de Biologia Molecular y Celular de Plantas, CSIC UPV, Valencia, Spain
    J Gen Virol 89:1739-47. 2008
    ..on each of the three genomic RNAs and it was found that recombination was very common in members of the genera Bromovirus, Cucumovirus and Ilarvirus...
  78. ncbi RNA-binding properties and mapping of the RNA-binding domain from the movement protein of Prunus necrotic ringspot virus
    M Carmen Herranz
    Instituto de Biología Molecular y Celular de Plantas IBMCP, UPV CSIC, Avda de los naranjos s n, 46022 Valencia, Spain
    J Gen Virol 85:761-8. 2004
    ..N terminus of their corresponding MPs, whereas similar domains previously characterized in members of the genera Bromovirus and Cucumovirus are present at the C terminus, strongly reflecting their corresponding phylogenetic ..
  79. ncbi Complete nucleotide sequence, genomic organization and phylogenetic analysis of Citrus leprosis virus cytoplasmic type
    E C Locali-Fabris
    Centro APTA Citros Sylvio Moreira, IAC, Rod Anhanguera, Km 158, CP 4, 13490 970 Cordeirópolis SP, Brazil
    J Gen Virol 87:2721-9. 2006
    ..and non-structural protein similarities revealed conserved domains with members of the genera Furovirus, Bromovirus, Tobravirus and Tobamovirus, although phylogenetic analyses strongly suggest that CiLV-C is a member of a ..
  80. ncbi The movement protein gene is involved in the virus-specific requirement of the coat protein in cell-to-cell movement of bromoviruses
    N Sasaki
    Laboratory of Plant Pathology, Graduate School of Agriculture, Kyoto University, Kyoto, Japan
    Arch Virol 148:803-12. 2003
    ..We thus conclude that the MP gene plays a crucial role in determination of the virus-specific CP requirement in bromovirus cell-to-cell movement.
  81. doi Quantitation of hepatitis delta virus using a single-step internally controlled real-time RT-qPCR and a full-length genomic RNA calibration standard
    R B Ferns
    Research Department of Infection, Division of Infection and Immunity, University College London, London, UK
    J Virol Methods 179:189-94. 2012
    ..4×10(4) to 4.4×10(8) copies/ml. The assay described provides a reliable and sensitive quantitative system for therapeutic monitoring and for studying the dynamic interplay between hepatitis B virus replication and HDV viral load...
  82. pmc Improved cell disruption of Pichia pastoris utilizing aminopropyl magnesium phyllosilicate (AMP) clay
    Sun Il Kim
    Department of Chemical and Biochemical Engineering, Chosun University, 375 Seosuk dong, Dong Gu, Gwangju 501 759, Republic of Korea
    World J Microbiol Biotechnol 29:1129-32. 2013
    ..The stability of purified CCMV VLPs illustrated AMP clay had no influence on virus assembly process. Considering the tiny amount added and simple approach of AMP clay, it could be a reliable method for yeast cell disruption...
  83. pmc Viral nanoparticles for in vivo tumor imaging
    Amy M Wen
    Department of Biomedical Engineering, Case Western Reserve University
    J Vis Exp . 2012
    ..We are further developing VNPs to carry drug cargos and clinically-relevant imaging moieties, as well as tissue-specific ligands to target molecular receptors overexpressed in cancer and cardiovascular disease...
  84. doi Reconstituted plant viral capsids can release genes to mammalian cells
    Odisse Azizgolshani
    Department of Chemistry and Biochemistry, University of California, Los Angeles CA 90095 1569, USA
    Virology 441:12-7. 2013
    ..Furthermore, the CCMV capsid protects the packaged RNA against nuclease degradation and serves as a robust external scaffold with many possibilities for further functionalization and cell targeting...
  85. ncbi RNA-RNA recombination in plant virus replication and evolution
    Joanna Sztuba-Solinska
    Plant Molecular Biology Center, Department of Biological Sciences, Northern Illinois University, DeKalb, Illinois 60115, USA
    Annu Rev Phytopathol 49:415-43. 2011
    ..The evidence for recombination, not only between related viruses but also among distantly related viruses, and even with host RNAs, suggests that plant viruses unabashedly test recombination with any genetic material at hand...
  86. doi Packaging and structural phenotype of brome mosaic virus capsid protein with altered N-terminal β-hexamer structure
    Melissanne de Wispelaere
    Department of Plant Pathology and Microbiology, University of California, Riverside, CA 92521 0122, USA
    Virology 419:17-23. 2011
    ..A comparison of 3-D models obtained by cryo electron microscopy revealed overall similar structural features for wild type and mutant virions, with small but significant differences near the 3-fold axes of symmetry...
  87. doi The coat protein leads the way: an update on basic and applied studies with the Brome mosaic virus coat protein
    C Cheng Kao
    Department of Molecular and Cellular Biochemistry, Indiana University, Bloomington, IN 47405, USA
    Mol Plant Pathol 12:403-12. 2011
    ..This update summarizes the interaction between the BMV CP and RNAs that can regulate RNA synthesis, translation and RNA encapsidation, as well as the formation of VLPs...
  88. pmc Magnetic virus-like nanoparticles in N. benthamiana plants: a new paradigm for environmental and agronomic biotechnological research
    Xinlei Huang
    Department of Chemistry, Indiana University, 800 E Kirkwood Avenue, Bloomington, Indiana 47405, United States
    ACS Nano 5:4037-45. 2011
    ..These particles potentially open new opportunities for high-contrast functional imaging in plants and for the delivery of therapeutic antimicrobial cores into plants...
  89. doi Controlled integration of polymers into viral capsids
    Marta Comellas-Aragonès
    Institute for Molecules and Materials, Radboud University Nijmegen, Nijmegen, The Netherlands
    Biomacromolecules 10:3141-7. 2009
    ..These are the first virus-like particles bearing synthetic organic polymers both inside and outside the viral capsid, opening a new route to the synthesis of biohybrid nanostructured materials based on viruses...
  90. doi The brome mosaic virus-based recombination vector triggers a limited gene silencing response depending on the orientation of the inserted sequence
    Andrzej Pacak
    Department of Gene Expression, Adam Mickiewicz University, Miedzychodzka 5, 60 371, Poznan, Poland
    Arch Virol 155:169-79. 2010
    ..The constructs containing a sense sequence or inverted repeats did not induce gene silencing but instead were eliminated from the plant cells...
  91. pmc Brome mosaic virus capsid protein regulates accumulation of viral replication proteins by binding to the replicase assembly RNA element
    Guanghui Yi
    Department of Biochemistry and Biophysics, Texas A and M University, College Station, 77843, USA
    RNA 15:615-26. 2009
    ..The B Box is also recognized by the BMV 1a protein to assemble the BMV replicase, suggesting that the BMV CP can act to regulate several viral infection processes...
  92. ncbi Genetic analysis of a host determination mechanism of bromoviruses in Arabidopsis thaliana
    Koki Fujisaki
    Laboratory of Plant Pathology, Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake cho, Sakyo ku, Kyoto 606 8502, Kyoto Prefecture, Japan
    Virus Res 140:103-11. 2009
    ....
  93. ncbi Sequence of cowpea chlorotic mottle virus RNAs 2 and 3 and evidence of a recombination event during bromovirus evolution
    R F Allison
    Institute of Molecular Virology, University of Wisconsin, Madison 53706
    Virology 172:321-30. 1989
    ....
  94. doi Depurination of Brome mosaic virus RNA3 in vivo results in translation-dependent accelerated degradation of the viral RNA
    Rikesh Gandhi
    Department of Biology, York University, Toronto, Ontario M3J 1P3, Canada
    J Biol Chem 283:32218-28. 2008
    ....
  95. ncbi Complex assembly behavior during the encapsulation of green fluorescent protein analogs in virus derived protein capsules
    Inge J Minten
    Radboud University Nijmegen, Institute for Molecules and Materials, Heyendaalseweg 135, 6525 AJ Nijmegen, The Netherlands
    Macromol Biosci 10:539-45. 2010
    ....
  96. doi Effects of amino-acid substitutions in the Brome mosaic virus capsid protein on RNA encapsidation
    Masarapu Hema
    Department of Molecular and Cellular Biochemistry, Indiana University, Bloomington, Indiana 47405, USA
    Mol Plant Microbe Interact 23:1433-47. 2010
    ..Furthermore, wild-type BMV RNA1 was found to be more susceptible to nuclease digestion relative to RNA2 as a function of the buffer pH. Other BMV capsid mutants also had altered ratios of packaged RNAs...
  97. doi Metal-ion-induced formation and stabilization of protein cages based on the cowpea chlorotic mottle virus
    Inge J Minten
    Radboud University Nijmegen, Institute for Molecules and Materials, Heyendaalseweg 135, 6525 AJ, Nijmegen, The Netherlands
    Small 7:911-9. 2011
    ..5. This metal-mediated stabilization methodology is employed to form stable capsids containing multiple proteins at pH 7.5, thereby greatly expanding the scope of the CCMV capsid as a nanoreactor...
  98. ncbi A simple technique for separation of Cowpea chlorotic mottle virus from Cucumber mosaic virus in natural mixed infections
    Akhtar Ali
    Department of Biological Science, University of Tulsa, Tulsa, OK 74104, USA
    J Virol Methods 153:163-7. 2008
    ..To our knowledge, this is the first report of a method separating CCMV directly from mixed infections with CMV in cowpea...
  99. doi The methyltransferase domain of the 1a protein of cowpea chlorotic mottle virus controls local and systemic accumulation in cowpea
    S Quan
    Department of Plant Pathology, Plant Sciences Building, The University of Georgia, Athens, GA 30602 7274, USA
    Arch Virol 153:1505-16. 2008
    ....
  100. ncbi Functional analysis of brome mosaic virus coat protein RNA-interacting domains
    S L Calhoun
    Department of Plant Pathology, University of California, Riverside, CA 92521 0122, USA
    Arch Virol 153:231-45. 2008
    The coat proteins (CP) of cowpea chlorotic mottle (CCMV) and brome mosaic virus (BMV), two members of the genus Bromovirus, share 70% identity at the amino acid (aa) level and contain four highly conserved regions, identified as putative ..
  101. pmc tRNA-like structure regulates translation of Brome mosaic virus RNA
    Sharief Barends
    UPR 9002 du CNRS, IBMC, F 67084 Strasbourg CEDEX, France
    J Virol 78:4003-10. 2004
    ..Brome mosaic virus (BMV), a bromovirus, has a tripartite RNA genome with a subgenomic RNA4 for coat protein expression...

Research Grants40

  1. GENE FUNCTIONS AND MOLECULAR GENETICS OF RNA VIRUSES
    Paul Ahlquist; Fiscal Year: 1992
    ..RNA recombination, which is thought to be a byproduct of RNA replication, demonstrated recombination between bromovirus deletion mutants will be used to determine how RNA sequence/structure features influence local recombination ..
  2. GENE FUNCTIONS AND MOLECULAR GENETICS OF RNA VIRUSES
    Paul Ahlquist; Fiscal Year: 1990
    ..RNA recombination, which is thought to be a byproduct of RNA replication, demonstrated recombination between bromovirus deletion mutants will be used to determine how RNA sequence/structure features influence local recombination ..
  3. GENE FUNCTIONS AND MOLECULAR GENETICS OF RNA VIRUSES
    Paul Ahlquist; Fiscal Year: 1991
    ..RNA recombination, which is thought to be a byproduct of RNA replication, demonstrated recombination between bromovirus deletion mutants will be used to determine how RNA sequence/structure features influence local recombination ..
  4. GENE FUNCTIONS AND MOLECULAR GENETICS OF RNA VIRUSES
    Paul Ahlquist; Fiscal Year: 1993
    ..RNA recombination, which is thought to be a byproduct of RNA replication, demonstrated recombination between bromovirus deletion mutants will be used to determine how RNA sequence/structure features influence local recombination ..
  5. GENE FUNCTIONS AND MOLECULAR GENETICS OF RNA VIRUSES
    Paul Ahlquist; Fiscal Year: 1999
    ..New approaches to analyze bromovirus encapsidation in vivo will be used to identify viral RNA encapsidation signals, and interactions between the ..
  6. GENE FUNCTIONS AND MOLECULAR GENETICS OF RNA VIRUSES
    Paul Ahlquist; Fiscal Year: 2009
    ..The results will advance understanding and control of widespread viral diseases, and understanding of normal cell functions relevant to other disease processes. ..
  7. In vivo imaging and dynamics of single virus-like particles
    Bogdan Dragnea; Fiscal Year: 2008
    ..We will learn unprecedented basic insight into virus biology. The technology will also broaden the area of future VLP applications and yield a new class of high-output probes that can be adapted to a wide range of targets. ..
  8. Regulation of Virus Capsid Assembly
    GILLIAN AIR; Fiscal Year: 2007
    ..25 million Americans. Worldwide, HBV will contribute to 1 million deaths this year. By determining how HBV assembly is regulated, we will characterize a new target for antiviral intervention. ..
  9. Roles of host RNA binding proteins in virus replication
    Peter Nagy; Fiscal Year: 2008
    ..The tractable in vitro and in vivo TBSV system developed by the investigator could prove beneficial to studies of other, less amenable RNA viruses. [unreadable] [unreadable] [unreadable]..
  10. Molecular Modulation of HBV Capsid Assembly
    Adam Zlotnick; Fiscal Year: 2008
    ..Worldwide, HBV will contribute to 1 million deaths this year. Current antiviral strategies focus on virus enzymes. We propose a new strategy for developing antiviral molecules that target virus assembly. ..
  11. GENE FUNCTIONS AND MOLECULAR GENETICS OF RNA VIRUSES
    Paul Ahlquist; Fiscal Year: 2008
    ..The results will advance understanding and control of widespread viral diseases, and understanding of normal cell functions relevant to other disease processes. ..
  12. RNA-dependent RNA Synthesis by the Hepatitis C Virus
    Cheng Kao; Fiscal Year: 2009
    ..The knowledge can also be used to compare the mechanisms of action of all template-dependent (both viral and cellular) polymerases. ..
  13. Multiscale modeling of mechanisms for viral capsid assembly and polymorphism
    Michael Hagan; Fiscal Year: 2009
    ..By identifying the features that make viral assembly successful, we can learn to block it and thereby create novel antivirus therapies. ..
  14. In vivo imaging and dynamics of single virus-like particles
    Bogdan Dragnea; Fiscal Year: 2009
    ..We will learn unprecedented basic insight into virus biology. The technology will also broaden the area of future VLP applications and yield a new class of high-output probes that can be adapted to a wide range of targets. ..
  15. Multiscale modeling of mechanisms for viral capsid assembly and polymorphism
    Michael F Hagan; Fiscal Year: 2010
    ..By identifying the features that make viral assembly successful, we can learn to block it and thereby create novel antivirus therapies. ..
  16. RNA-dependent RNA Synthesis by the Hepatitis C Virus
    CHENG C KAO; Fiscal Year: 2010
    ..The knowledge can also be used to compare the mechanisms of action of all template-dependent (both viral and cellular) polymerases. ..
  17. Multiscale modeling of mechanisms for viral capsid assembly and polymorphism
    Michael Hagan; Fiscal Year: 2009
    ..By identifying the features that make viral assembly successful, we can learn to block it and thereby create novel antivirus therapies. ..
  18. In vivo imaging and dynamics of single virus-like particles
    Bogdan Dragnea; Fiscal Year: 2010
    ..We will learn unprecedented basic insight into virus biology. The technology will also broaden the area of future VLP applications and yield a new class of high-output probes that can be adapted to a wide range of targets. ..
  19. Molecular biology of viral capsids
    CHENG C KAO; Fiscal Year: 2010
    ..The information gained from the proposed studies will contribute new insights to virus architecture, protein-RNA interactions, and how viruses gain entry into the cell. ..
  20. Multiscale modeling of mechanisms for viral capsid assembly and polymorphism
    Michael F Hagan; Fiscal Year: 2011
    ..By identifying the features that make viral assembly successful, we can learn to block it and thereby create novel antivirus therapies. ..
  21. In vivo imaging and dynamics of single virus-like particles
    Bogdan Dragnea; Fiscal Year: 2007
    ..We will learn unprecedented basic insight into virus biology. The technology will also broaden the area of future VLP applications and yield a new class of high-output probes that can be adapted to a wide range of targets. ..
  22. Roles of host RNA binding proteins in virus replication
    Peter Nagy; Fiscal Year: 2007
    ..The tractable in vitro and in vivo TBSV system developed by the investigator could prove beneficial to studies of other, less amenable RNA viruses. [unreadable] [unreadable] [unreadable]..
  23. Novel Approaches to Detect Virus-Cancer Associations
    Paul Ahlquist; Fiscal Year: 2002
    ..The methods developed also will be applicable to probe for virus involvement in other chronic or acute disorders. ..
  24. Novel Approaches to Detect Virus-Cancer Associations
    Paul Ahlquist; Fiscal Year: 2003
    ..The methods developed also will be applicable to probe for virus involvement in other chronic or acute disorders. ..
  25. RPA STRUCTURE AND MECHANISM OF INTERACTION WITH SSDNA
    Adam Zlotnick; Fiscal Year: 2004
    ..4. What is the general mechanism of ssDNA-binding by the RPA trimer? A trimeric RPA complex containing all ssDNA-binding domains will be resolved by X-ray crystallography with and/or without ssDNA. ..
  26. Novel Approaches to Detect Virus-Cancer Associations
    Paul Ahlquist; Fiscal Year: 2004
    ..The methods developed also will be applicable to probe for virus involvement in other chronic or acute disorders. ..
  27. Dissecting the mechanism of viral RNA recombination
    Peter Nagy; Fiscal Year: 2004
    ..Knowledge of recombination promises untold health benefits by providing means to limit virus infections and safer vaccines. ..
  28. Novel Approaches to Detect Virus-Cancer Associations
    Paul Ahlquist; Fiscal Year: 2005
    ..The methods developed also will be applicable to probe for virus involvement in other chronic or acute disorders. ..
  29. GENE FUNCTIONS AND MOLECULAR GENETICS OF RNA VIRUSES
    Paul Ahlquist; Fiscal Year: 2006
    ..The results will advance understanding and control of widespread viral diseases, and understanding of normal cell functions relevant to other disease processes. [unreadable] [unreadable]..
  30. Host factors involved in viral RNA recombination
    Peter Nagy; Fiscal Year: 2006
    ..Knowledge of recombination promises untold health benefits by providing means to limit virus infections and to develop safer vaccines. ..
  31. Role of an essential RNA chaperone in virus replication
    Peter Nagy; Fiscal Year: 2006
    ....
  32. GENE FUNCTIONS AND MOLECULAR GENETICS OF RNA VIRUSES
    Paul Ahlquist; Fiscal Year: 2007
    ..The results will advance understanding and control of widespread viral diseases, and understanding of normal cell functions relevant to other disease processes. [unreadable] [unreadable]..
  33. Molecular Modulation of HBV Capsid Assembly
    Adam Zlotnick; Fiscal Year: 2007
    ..Worldwide, HBV will contribute to 1 million deaths this year. Current antiviral strategies focus on virus enzymes. We propose a new strategy for developing antiviral molecules that target virus assembly. [unreadable] [unreadable]..
  34. Multiscale modeling of mechanisms for viral capsid assembly and polymorphism
    Michael F Hagan; Fiscal Year: 2010
    ..By identifying the features that make viral assembly successful, we can learn to block it and thereby create novel antivirus therapies. ..
  35. Molecular Modulation of HBV Capsid Assembly
    Adam Zlotnick; Fiscal Year: 2008
    ..25 million Americans. Worldwide, HBV will contribute to 1 million deaths this year. Current antiviral strategies focus on virus enzymes. We propose a new strategy for developing antiviral molecules that target virus assembly. ..