simian virus 40

Summary

Summary: A species of POLYOMAVIRUS originally isolated from Rhesus monkey kidney tissue. It produces malignancy in human and newborn hamster kidney cell cultures.

Top Publications

  1. ncbi Simian Virus 40 depends on ER protein folding and quality control factors for entry into host cells
    Mario Schelhaas
    Institute of Biochemistry, Schafmattstrasse 18, ETH Hoenggerberg, ETH Zurich, CH 8093, Zurich, Switzerland
    Cell 131:516-29. 2007
  2. pmc Clathrin- and caveolin-1-independent endocytosis: entry of simian virus 40 into cells devoid of caveolae
    Eva Maria Damm
    Institute of Biochemistry, Swiss Federal Institute of Technology Zurich ETHZ, CH 8093 Zurich, Switzerland
    J Cell Biol 168:477-88. 2005
  3. ncbi GM1 structure determines SV40-induced membrane invagination and infection
    Helge Ewers
    ETH Zurich, Institute of Biochemistry, HPM E, Schafmattstrasse 18, 8093 Zurich, Switzerland
    Nat Cell Biol 12:11-8; sup pp 1-12. 2010
  4. pmc SV40 DNA replication: from the A gene to a nanomachine
    Ellen Fanning
    Department of Biological Sciences, Vanderbilt Ingram Cancer Center, Vanderbilt University, Nashville, TN 37235 1634, USA
    Virology 384:352-9. 2009
  5. ncbi PML-nuclear bodies accumulate DNA in response to polyomavirus BK and simian virus 40 replication
    Asne Jul-Larsen
    Section of Anatomy and Cell Biology, Department of Biomedicine, University of Bergen, 5009, Norway
    Exp Cell Res 298:58-73. 2004
  6. pmc Structural basis for the interaction of a hexameric replicative helicase with the regulatory subunit of human DNA polymerase α-primase
    Bo Zhou
    Department of Molecular and Computational Biology, University of Southern California, Los Angeles, California 90089, USA
    J Biol Chem 287:26854-66. 2012
  7. pmc Frequent detection of polyomaviruses in stool samples from hospitalized children
    John A Vanchiere
    Department of Pediatrics, Section of Infectious Diseases, Baylor College of Medicine, Houston, Texas, USA
    J Infect Dis 192:658-64. 2005
  8. pmc Ataxia telangiectasia-mutated damage-signaling kinase- and proteasome-dependent destruction of Mre11-Rad50-Nbs1 subunits in Simian virus 40-infected primate cells
    Xiaorong Zhao
    Department of Biological Sciences, Vanderbilt University, Nashville, Tennessee 37235 1634, USA
    J Virol 82:5316-28. 2008
  9. pmc Induction of interferon-stimulated genes by Simian virus 40 T antigens
    Abhilasha V Rathi
    Department of Biological Sciences, University of Pittsburgh, 559 Crawford Hall, Pittsburgh, PA 15260, USA
    Virology 406:202-11. 2010
  10. ncbi Uncatalyzed assembly of spherical particles from SV40 VP1 pentamers and linear dsDNA incorporates both low and high cooperativity elements
    Santanu Mukherjee
    Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA
    Virology 397:199-204. 2010

Research Grants

  1. Screens for host DNA replication and repair factors involved in viral infection
    Matthew D Weitzman; Fiscal Year: 2010
  2. T-antigen Binding to the Merkel Cell Carcinoma Virus Origin
    ALEX ANDREW BOHM; Fiscal Year: 2010
  3. CONTROL OF SIMIAN VIRUS 40 AND CELLULAR DNA REPLICATION
    KATHERINE LOUISE FRIEDMAN; Fiscal Year: 2013
  4. Expression of Simiam Virus 40 T Antigens in Intestine
    James M Pipas; Fiscal Year: 2013
  5. Pathogenesis of mesothelioma
    Michele Carbone; Fiscal Year: 2010
  6. Simian Virus 40 (SV40) uncoating and penetration
    Daniel Hebert; Fiscal Year: 2009
  7. Role of microRNAs in the SV40 infectious cycle
    Christopher S Sullivan; Fiscal Year: 2012
  8. HTLV-III SEQUENCES AT THE TRANSCRIPTIONAL LEVEL
    JOSEPH MOSCA; Fiscal Year: 1991
  9. MECHANISMS OF NUCLEAR PROTEIN ACCUMULATION
    PHILIP PAINE; Fiscal Year: 1993
  10. Hyperacetylation of Biologically Active SV40 Chromatin
    Barry Milavetz; Fiscal Year: 2006

Detail Information

Publications280 found, 100 shown here

  1. ncbi Simian Virus 40 depends on ER protein folding and quality control factors for entry into host cells
    Mario Schelhaas
    Institute of Biochemistry, Schafmattstrasse 18, ETH Hoenggerberg, ETH Zurich, CH 8093, Zurich, Switzerland
    Cell 131:516-29. 2007
    Cell entry of Simian Virus 40 (SV40) involves caveolar/lipid raft-mediated endocytosis, vesicular transport to the endoplasmic reticulum (ER), translocation into the cytosol, and import into the nucleus...
  2. pmc Clathrin- and caveolin-1-independent endocytosis: entry of simian virus 40 into cells devoid of caveolae
    Eva Maria Damm
    Institute of Biochemistry, Swiss Federal Institute of Technology Zurich ETHZ, CH 8093 Zurich, Switzerland
    J Cell Biol 168:477-88. 2005
    b>Simian Virus 40 (SV40) has been shown to enter host cells by caveolar endocytosis followed by transport via caveosomes to the endoplasmic reticulum (ER)...
  3. ncbi GM1 structure determines SV40-induced membrane invagination and infection
    Helge Ewers
    ETH Zurich, Institute of Biochemistry, HPM E, Schafmattstrasse 18, 8093 Zurich, Switzerland
    Nat Cell Biol 12:11-8; sup pp 1-12. 2010
    Incoming simian virus 40 (SV40) particles enter tight-fitting plasma membrane invaginations after binding to the carbohydrate moiety of GM1 gangliosides in the host cell plasma membrane through pentameric VP1 capsid proteins...
  4. pmc SV40 DNA replication: from the A gene to a nanomachine
    Ellen Fanning
    Department of Biological Sciences, Vanderbilt Ingram Cancer Center, Vanderbilt University, Nashville, TN 37235 1634, USA
    Virology 384:352-9. 2009
    Duplication of the simian virus 40 (SV40) genome is the best understood eukaryotic DNA replication process to date. Like most prokaryotic genomes, the SV40 genome is a circular duplex DNA organized in a single replicon...
  5. ncbi PML-nuclear bodies accumulate DNA in response to polyomavirus BK and simian virus 40 replication
    Asne Jul-Larsen
    Section of Anatomy and Cell Biology, Department of Biomedicine, University of Bergen, 5009, Norway
    Exp Cell Res 298:58-73. 2004
    ..labeling to demonstrate that PML-NBs accumulate newly synthesized DNA in cells infected by the polyomaviruses simian virus 40 (SV40) or polyomavirus BK (BKV)...
  6. pmc Structural basis for the interaction of a hexameric replicative helicase with the regulatory subunit of human DNA polymerase α-primase
    Bo Zhou
    Department of Molecular and Computational Biology, University of Southern California, Los Angeles, California 90089, USA
    J Biol Chem 287:26854-66. 2012
    ....
  7. pmc Frequent detection of polyomaviruses in stool samples from hospitalized children
    John A Vanchiere
    Department of Pediatrics, Section of Infectious Diseases, Baylor College of Medicine, Houston, Texas, USA
    J Infect Dis 192:658-64. 2005
    ..PCR) assay that could detect a conserved region in the large T antigen gene of BKV, JC virus (JCV), and simian virus 40 (SV40)...
  8. pmc Ataxia telangiectasia-mutated damage-signaling kinase- and proteasome-dependent destruction of Mre11-Rad50-Nbs1 subunits in Simian virus 40-infected primate cells
    Xiaorong Zhao
    Department of Biological Sciences, Vanderbilt University, Nashville, Tennessee 37235 1634, USA
    J Virol 82:5316-28. 2008
    Although the mechanism of simian virus 40 (SV40) DNA replication has been extensively investigated with cell extracts, viral DNA replication in productively infected cells utilizes additional viral and host functions whose interplay ..
  9. pmc Induction of interferon-stimulated genes by Simian virus 40 T antigens
    Abhilasha V Rathi
    Department of Biological Sciences, University of Pittsburgh, 559 Crawford Hall, Pittsburgh, PA 15260, USA
    Virology 406:202-11. 2010
    b>Simian virus 40 (SV40) large T antigen (TAg) is a multifunctional oncoprotein essential for productive viral infection and for cellular transformation...
  10. ncbi Uncatalyzed assembly of spherical particles from SV40 VP1 pentamers and linear dsDNA incorporates both low and high cooperativity elements
    Santanu Mukherjee
    Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA
    Virology 397:199-204. 2010
    ..These data suggest a DNA saturation-dependent nucleation event based on non-specific pentamer-DNA interaction that controls assembly and the ultimate capsid geometry...
  11. pmc Simian virus 40 infection triggers a balanced network that includes apoptotic, survival, and stress pathways
    Veronika Butin-Israeli
    Department of Hematology, Hebrew University Hadassah Medical School, Jerusalem, Israel 91120
    J Virol 84:3431-42. 2010
    The infection process by simian virus 40 (SV40) and entry of its genome into nondividing cells are only partly understood...
  12. ncbi Specific antibodies reacting with simian virus 40 capsid protein mimotopes in serum samples from healthy blood donors
    Alfredo Corallini
    Section of Microbiology, University of Ferrara, 44121 Ferrara, Italy
    Hum Immunol 73:502-10. 2012
    b>Simian virus 40 (SV40), a small DNA tumor virus, was inadvertently administered to human populations with the use of contaminated vaccines...
  13. pmc Formation of transitory intrachain and interchain disulfide bonds accompanies the folding and oligomerization of simian virus 40 Vp1 in the cytoplasm
    Peggy P Li
    Molecular Biology Institute and Department of Molecular, Cell, and Developmental Biology, University of California, 405 Hilgard Avenue, Los Angeles, CA 90095, USA
    Proc Natl Acad Sci U S A 99:1353-8. 2002
    Pentamer formation by Vp1, the major capsid protein of simian virus 40, requires an interdigitation of structural elements from the Vp1 monomers [Liddington, R. C., Yan, Y., Moulai, J., Sahli, R., Benjamin, T. L. & Harrison, S. C...
  14. pmc A distinct first replication cycle of DNA introduced in mammalian cells
    Gurangad S Chandok
    Department of Biochemistry and Molecular Biology, Penn State University, University Park, PA 16801, USA
    Nucleic Acids Res 39:2103-15. 2011
    ..This work demonstrates, for the first time, differences in the replication progression during the first and subsequent replication cycles in mammalian cells...
  15. pmc Simian virus 40 small T antigen activates AMPK and triggers autophagy to protect cancer cells from nutrient deprivation
    Sravanth Hindupur Kumar
    Department of Molecular Reproduction, Development, and Genetics, Indian Institute of Science, Bangalore 560012, India
    J Virol 83:8565-74. 2009
    ..Using an in vitro transformation model where the presence of the simian virus 40 (SV40) small T (ST) antigen has been shown to be critical for tumorigenic transformation, we investigated ..
  16. pmc Simian virus 40 large T antigen disrupts genome integrity and activates a DNA damage response via Bub1 binding
    Jennifer Hein
    Hillman Cancer Center, Research Pavilion Suite 1 8, 5117 Centre Avenue, Pittsburgh, PA 15213, USA
    J Virol 83:117-27. 2009
    b>Simian virus 40 (SV40) large T antigen (LT) is a multifunctional protein that is important for viral replication and oncogenic transformation...
  17. pmc Multiple DNA damage signaling and repair pathways deregulated by simian virus 40 large T antigen
    Sergei Boichuk
    University of Pittsburgh Cancer Institute, Pittsburgh, Pennsylvania 15213, USA
    J Virol 84:8007-20. 2010
    We demonstrated previously that expression of simian virus 40 (SV40) large T antigen (LT), without a viral origin, is sufficient to induce the hallmarks of a cellular DNA damage response (DDR), such as focal accumulation of gamma-H2AX ..
  18. pmc Interaction of the Vp3 nuclear localization signal with the importin alpha 2/beta heterodimer directs nuclear entry of infecting simian virus 40
    Akira Nakanishi
    Department of Molecular, Cell, and Developmental Biology and Molecular Biology Institute, University of California at Los Angeles, Los Angeles, California 90095, USA
    J Virol 76:9368-77. 2002
    ..We show that the nuclear entry of simian virus 40 involves a dynamic interplay between two distinct interiorly situated capsid NLSs, the Vp1 NLS and the Vp3 ..
  19. pmc Role of endosomes in simian virus 40 entry and infection
    Sabrina Engel
    ETH Zurich, Institute of Biochemistry, Schafmattstrasse 18, 8093 Zurich, Switzerland
    J Virol 85:4198-211. 2011
    After binding to its cell surface receptor ganglioside GM1, simian virus 40 (SV40) is endocytosed by lipid raft-mediated endocytosis and slowly transported to the endoplasmic reticulum, where partial uncoating occurs...
  20. ncbi Hepatitis C virus NS3 and simian virus 40 T antigen helicases displace streptavidin from 5'-biotinylated oligonucleotides but not from 3'-biotinylated oligonucleotides: evidence for directional bias in translocation on single-stranded DNA
    Patrick D Morris
    Department of Biochemistry and Molecular Biology, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205, USA
    Biochemistry 41:2372-8. 2002
    ..The dependence of the reaction with NS3 on the oligonucleotide length suggests that multiple NS3 monomers are necessary for optimal activity...
  21. pmc Caveolar endocytosis of simian virus 40 is followed by brefeldin A-sensitive transport to the endoplasmic reticulum, where the virus disassembles
    Leonard C Norkin
    Department of Microbiology, University of Massachusetts, Amherst, Massachusetts 01003 5720, USA
    J Virol 76:5156-66. 2002
    b>Simian virus 40 (SV40) enters cells by atypical endocytosis mediated by caveolae that transports the virus to the endoplasmic reticulum (ER) instead of to the endosomal-lysosomal compartment, which is the usual destination for viruses ..
  22. ncbi Imaging viral behavior in Mammalian cells with self-assembled capsid-quantum-dot hybrid particles
    Feng Li
    State Key Laboratory of Virology, Wuhan Institute of Virology Chinese Academy of Sciences No 44, Xiaohongshan, Wuhan 430071, China
    Small 5:718-26. 2009
    ..movement, and localization of viruses in living cells, QD-containing virus-like particles (VLPs) of simian virus 40 (SV40), termed SVLP-QDs, are constructed by in vitro self-assembly of the major capsid protein of SV40...
  23. ncbi Viral coat proteins as flexible nano-building-blocks for nanoparticle encapsulation
    Feng Li
    State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, No 44, Xiaohongshan, Wuhan 430071, PR China
    Small 6:2301-8. 2010
    ..We previously generated virus-based nanoparticles (VNPs) of simian virus 40 (SV40) containing quantum dots (QDs) for cellular imaging...
  24. pmc Bypass of a protein barrier by a replicative DNA helicase
    Hasan Yardimci
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 492:205-9. 2012
    ..By contrast, large T antigen, the replicative DNA helicase of the simian virus 40 (SV40), is reported to function as a pair of stacked hexamers that pumps double-stranded DNA through its ..
  25. pmc Simian virus 40 activates ATR-Delta p53 signaling to override cell cycle and DNA replication control
    Gabor Rohaly
    Heinrich Pette Institut für Experimentelle Virologie und Immunologie, Martinistr 52, D 20251 Hamburg, Germany
    J Virol 84:10727-47. 2010
    During infection, simian virus 40 (SV40) attempts to take hold of the cell, while the host responds with various defense systems, including the ataxia-telangiectasia mutated/ATM-Rad3 related (ATM/ATR)-mediated DNA damage response ..
  26. ncbi Lipid rafts and caveolae as portals for endocytosis: new insights and common mechanisms
    Robert G Parton
    Institute for Molecular Bioscience, Centre for Microscopy and Microanalysis, and School of Biomedical Sciences, University of Queensland, Queensland, 4072, Australia
    Traffic 4:724-38. 2003
    ..This review examines the current evidence for the involvement of rafts and caveolae in endocytosis and the molecular players involved in their regulation...
  27. pmc Conformational rearrangements of SV40 large T antigen during early replication events
    Isabel Cuesta
    Unidad de Biocomputación, Centro Nacional de Biotecnologia CSIC, C Darwin 3, 28049 Madrid, Spain
    J Mol Biol 397:1276-86. 2010
    The Simian virus 40 (SV40) large tumor antigen (LTag) functions as the replicative helicase and initiator for viral DNA replication...
  28. ncbi SV40 multiple tissue infection and asbestos exposure in a hyperendemic area for malignant mesothelioma
    Manola Comar
    Department of Public Medicine Sciences, University of Trieste, Trieste, Italy
    Cancer Res 67:8456-9. 2007
    ..045). This survey shows that SV40 sustains infections in multiple tissues in malignant mesothelioma patients from a geographic area affected with asbestos-related mesothelioma...
  29. pmc Adenovirus E1A, simian virus 40 tumor antigen, and human papillomavirus E7 protein share the capacity to disrupt the interaction between transcription factor E2F and the retinoblastoma gene product
    S Chellappan
    Howard Hughes Medical Institute, Department of Microbiology and Immunology, Duke University Medical Center, Durham, NC 27710
    Proc Natl Acad Sci U S A 89:4549-53. 1992
    The adenovirus E1A gene product, the simian virus 40 large tumor antigen, and the human papillomavirus E7 protein share a short amino acid sequence that constitutes a domain required for the transforming activity of these proteins...
  30. pmc Association of simian virus 40 vp1 with 70-kilodalton heat shock proteins and viral tumor antigens
    Peggy P Li
    Molecular Biology Institute, 456 Boyer Hall, University of California at Los Angeles, 611 East Charles E Young Dr, Box 951570, Los Angeles, CA 90095 1570, USA
    J Virol 83:37-46. 2009
    ..The major capsid protein Vp1 of simian virus 40 forms a series of disulfide-linked intermediates during folding and capsid formation...
  31. pmc Simian virus 40 Vp1 DNA-binding domain is functionally separable from the overlapping nuclear localization signal and is required for effective virion formation and full viability
    P P Li
    Department of Molecular, Cell and Developmental Biology and Molecular Biology Institute, University of California at Los Angeles, Los Angeles, California 90095, USA
    J Virol 75:7321-9. 2001
    A DNA-binding domain (DBD) was identified on simian virus 40 (SV40) major capsid protein Vp1, and the domain's function in the SV40 life cycle was examined...
  32. ncbi Detection of BK virus and simian virus 40 in the urine of healthy children
    John A Vanchiere
    Department of Pediatrics, Section of Infectious Diseases, Baylor College of Medicine, Houston, Texas 77030, USA
    J Med Virol 75:447-54. 2005
    ..We sought to determine if simian virus 40 (SV40) excretion could be detected in the urine of healthy children and to define the age-related prevalence ..
  33. ncbi Inhibition of Simian virus 40 large T antigen helicase activity by fluoroquinolones
    Syed Hamid Ali
    Department of Biological and Biomedical Sciences, The Aga Khan University, Karachi, Pakistan
    Antivir Ther 12:1-6. 2007
    ..Inhibition of gyrase activity by quinolones involves the interaction of these drugs with the helicase component of bacterial gyrase. DNA tumour viruses also encode helicases that are essential for their DNA replication in the host...
  34. pmc SR alpha promoter: an efficient and versatile mammalian cDNA expression system composed of the simian virus 40 early promoter and the R-U5 segment of human T-cell leukemia virus type 1 long terminal repeat
    Y Takebe
    Department of Molecular Biology, DNAX Research Institute of Molecular and Cellular Biology, Palo Alto, California 94304
    Mol Cell Biol 8:466-72. 1988
    We developed a novel promoter system, designated SR alpha, which is composed of the simian virus 40 (SV40) early promoter and the R segment and part of the U5 sequence (R-U5') of the long terminal repeat of human T-cell leukemia virus ..
  35. ncbi Clonal chromosomal aberrations in simian virus 40-transfected human thyroid cells and in derived tumors developed after in vitro irradiation
    H Zitzelsberger
    Institute of Radiobiology, GSF Forschungszentrum für Umwelt und Gesundheit GmbH, Neuherberg, Germany
    Int J Cancer 96:166-77. 2001
    ..A number of chromosomal aberrations were found to be characteristic for simian virus 40 immortalization and/or radiation-induced transformation of human thyroid epithelial cells...
  36. pmc Effects of simian virus 40 large and small tumor antigens on mammalian target of rapamycin signaling: small tumor antigen mediates hypophosphorylation of eIF4E-binding protein 1 late in infection
    Yongjun Yu
    Department of Cancer Biology, Abramson Family Cancer Research Institute, University of Pennsylvania, Philadelphia, PA 19104 6142, USA
    J Virol 79:6882-9. 2005
    We report that late in a simian virus 40 (SV40) infection in CV-1 cells, there are significant decreases in phosphorylations of two mammalian target of rapamycin (mTOR) signaling effectors, the eIF4E-binding protein (4E-BP1) and p70 S6 ..
  37. pmc Identification of a promoter component involved in positioning the 5' termini of simian virus 40 early mRNAs
    P K Ghosh
    Proc Natl Acad Sci U S A 78:100-4. 1981
    The 5' termini of the principal early mRNAs produced in cells transformed by wild-type simian virus 40 lie 21-25 nucleotides downstream from an A-T-T-T-A-T sequence on the DNA template...
  38. pmc Activation of the tumor-specific death effector apoptin and its kinase by an N-terminal determinant of simian virus 40 large T antigen
    Ying Hui Zhang
    Leadd B V, Leiden, The Netherlands
    J Virol 78:9965-76. 2004
    ..cells apoptin can also be activated by the transient transforming signals conferred by ectopically expressed simian virus 40 (SV40) large T antigen (LT), which rapidly induces apoptin's phosphorylation, nuclear accumulation, and the ..
  39. ncbi SV40 and human tumours: myth, association or causality?
    Adi F Gazdar
    Hamon Center for Therapeutic Oncology Research and Department of Pathology, University of Texas Southwestern Medical Centre, Dallas, Texas 75390, USA
    Nat Rev Cancer 2:957-64. 2002
    An increasing number of scientific reports have described evidence for a polyomavirus, simian virus 40, in a highly select group of human tumours...
  40. ncbi Caveolin-stabilized membrane domains as multifunctional transport and sorting devices in endocytic membrane traffic
    Lucas Pelkmans
    Max Planck Institute for Molecular Cell Biology and Genetics, 01307 Dresden, Germany
    Cell 118:767-80. 2004
    ..Following transport of the caveolar protein Caveolin-1 and two cargo complexes, Simian Virus 40 and Cholera toxin, in live cells, we uncovered a Rab5-dependent pathway in which caveolar vesicles are ..
  41. ncbi Exploitation of major histocompatibility complex class I molecules and caveolae by simian virus 40
    R G Parton
    Department of Physiology and Pharmacology, University of Queensland, Brisbane, Australia
    Immunol Rev 168:23-31. 1999
    b>Simian virus 40 (SV40), a non-enveloped DNA virus, is transported from the cell surface to the nucleus where virus replication occurs...
  42. ncbi Cancer risk associated with receipt of vaccines contaminated with simian virus 40: epidemiologic research
    Eric A Engels
    Division of Cancer Epidemiology and Genetics, National Cancer Institute, DHHS, 6120 Executive Blvd, EPS 8010 Rockville, MD 20892, USA
    Expert Rev Vaccines 4:197-206. 2005
    ....
  43. ncbi Absence of Epstein-Barr virus, human papillomavirus, and simian virus 40 in patients of central european origin with lymphoepithelioma-like carcinoma of the skin
    Dmitry V Kazakov
    Sikl s Department of Pathology, Charles University, Medical Faculty Hospital, Pilsen, Czech Republic
    Am J Dermatopathol 29:365-9. 2007
    ..Because all 10 cases studied proved negative for EBV, HPV, and SV40, these viruses seem to play no causal role in LELC of the skin in patients from Central Europe...
  44. ncbi Maximum-likelihood multi-reference refinement for electron microscopy images
    Sjors H W Scheres
    Biocomputing Unit, Centro Nacional de Biotecnologia, Campus Universidad Autonoma, Cantoblanco, 28049, Madrid, Spain
    J Mol Biol 348:139-49. 2005
    ..of this approach to a cryo-electron microscopy dataset revealed two major classes for projections of simian virus 40 large T-antigen in complex with an asymmetric DNA-probe, containing the origin of simian virus 40 replication...
  45. ncbi Secrets of caveolae- and lipid raft-mediated endocytosis revealed by mammalian viruses
    Lucas Pelkmans
    Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany
    Biochim Biophys Acta 1746:295-304. 2005
    ..Again, viruses are proving to be excellent tools to reach that goal...
  46. pmc Stat3 is required for full neoplastic transformation by the Simian Virus 40 large tumor antigen
    Adina Vultur
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario K7L 3N6, Canada
    Mol Biol Cell 16:3832-46. 2005
    ..transducer and activator of transcription-3) in neoplastic transformation by the Large Tumor antigen of Simian Virus 40 (TAg), murine fibroblasts were rendered deficient in Stat3 activity through expression of a Stat3-specific ..
  47. pmc Histone hyperacetylation in the coding region of chromatin undergoing transcription in SV40 minichromosomes is a dynamic process regulated directly by the presence of RNA polymerase II
    Lata Balakrishnan
    Department of Biochemistry and Molecular Biology, University of North Dakota, Grand Forks, ND 58203, USA
    J Mol Biol 365:18-30. 2007
    ....
  48. pmc The T/t common exon of simian virus 40, JC, and BK polyomavirus T antigens can functionally replace the J-domain of the Escherichia coli DnaJ molecular chaperone
    W L Kelley
    Departement de Biochimie Medicale, Centre Medical Universitaire, Universite de Geneve, Geneva, Switzerland
    Proc Natl Acad Sci U S A 94:3679-84. 1997
    ..yet significant, amino acid sequence homology to the J-domain, the DNA encoding the T/t common exon of the simian virus 40 (SV40), JC, or BK polyoma virus T antigen oncoproteins was used to construct J-domain replacement chimeras of ..
  49. pmc Crocidolite asbestos and SV40 are cocarcinogens in human mesothelial cells and in causing mesothelioma in hamsters
    Barbara Kroczynska
    Thoracic Oncology Program, Cardinal Bernardin Cancer Center, Loyola University Chicago, Maywood, IL 60153, USA
    Proc Natl Acad Sci U S A 103:14128-33. 2006
    ..Our results indicate that mineral fibers and viruses can be cocarcinogens and suggest that lower amounts of asbestos may be sufficient to cause MM in individuals infected with SV40...
  50. pmc Cell-type specific regulation of gene expression by simian virus 40 T antigens
    Paul G Cantalupo
    Department of Biological Sciences, 559 Crawford Hall, University of Pittsburgh Pittsburgh, Pennsylvania 15260, USA
    Virology 386:183-91. 2009
    ....
  51. pmc Cell penetration and trafficking of polyomavirus
    Joanna M Gilbert
    Department of Pathology, Harvard Medical School, 200 Longwood Avenue, Armenise 233, Boston, MA 02115, USA
    J Virol 77:2615-22. 2003
    ..In contrast, uptake of simian virus 40 into the same cells is dependent on caveola...
  52. pmc A large and intact viral particle penetrates the endoplasmic reticulum membrane to reach the cytosol
    Takamasa Inoue
    Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, Michigan, United States of America
    PLoS Pathog 7:e1002037. 2011
    ..They also suggest that the ER membrane supports passage of a large particle, potentially through either a sizeable protein-conducting channel or the lipid bilayer...
  53. pmc Enumeration of the simian virus 40 early region elements necessary for human cell transformation
    William C Hahn
    Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, USA
    Mol Cell Biol 22:2111-23. 2002
    ..We recently reported that coexpression of the simian virus 40 (SV40) early region (ER), the gene encoding the telomerase catalytic subunit (hTERT), and an oncogenic allele ..
  54. ncbi Ataxia-telangiectasia-mutated (ATM) is a T-antigen kinase that controls SV40 viral replication in vivo
    Yuling Shi
    Department of Pharmacology, University of Wisconsin Medical School, Madison, Wisconsin 53706, USA
    J Biol Chem 280:40195-200. 2005
    ..Our findings uncover a direct link between ATM and SV40 LTag that may have implications for understanding the replication cycle of oncogenic polyoma viruses...
  55. pmc Association with capsid proteins promotes nuclear targeting of simian virus 40 DNA
    A Nakanishi
    Department of Molecular, Cell, and Developmental Biology, University of California, Los Angeles 90095, USA
    Proc Natl Acad Sci U S A 93:96-100. 1996
    ..The role of capsid proteins in nuclear targeting of simian virus 40 (SV40) DNA, which is assessed by the nuclear accumulation of large tumor (T) antigen, the initial sign of the ..
  56. pmc A very late viral protein triggers the lytic release of SV40
    Robert Daniels
    Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst, Massachusetts, United States of America
    PLoS Pathog 3:e98. 2007
    How nonenveloped viruses such as simian virus 40 (SV40) trigger the lytic release of their progeny is poorly understood...
  57. pmc Sequence requirements for plasmid nuclear import
    D A Dean
    College of Medicine, University of South Alabama, Mobile, Alabama 36688, USA
    Exp Cell Res 253:713-22. 1999
    ..Thus, the inclusion this SV40 sequence in nonviral vectors may greatly increase their ability to be transported into the nucleus, especially in nondividing cells...
  58. ncbi Asbestos and SV40 in malignant pleural mesothelioma from a hyperendemic area of north-eastern Italy
    Manola Comar
    Institute for Maternal and Child Health, IRCCS Burlo Garofolo, Trieste, University of Trieste, Trieste, Italy
    Tumori 98:210-4. 2012
    ....
  59. ncbi The simian virus 40 large tumor antigen activates cSrc and requires cSrc for full neoplastic transformation
    Rozanne Arulanandam
    Department of Microbiology and Immunology, Queen s University, Botterell Hall, Rm 713, Kingston, Ontario K7L3N6, Canada
    Anticancer Res 30:47-53. 2010
    To investigate the role of the cellular protooncogene product, cSrc, in neoplastic transformation by the large tumor antigen of simian virus 40 (TAg), the ability of TAg to increase cSrc activity was examined.
  60. pmc Effects of T antigen and replication protein A on the initiation of DNA synthesis by DNA polymerase alpha-primase
    K L Collins
    Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205
    Mol Cell Biol 11:2108-15. 1991
    Studies of simian virus 40 (SV40) DNA replication in a reconstituted cell-free system have established that T antigen and two cellular replication proteins, replication protein A (RP-A) and DNA polymerase alpha-primase complex, are ..
  61. pmc Studies on the initiation and elongation reactions in the simian virus 40 DNA replication system
    T Matsumoto
    Graduate Program in Molecular Biology, Memorial Sloan Kettering Cancer Center, Sloan Kettering Institute, New York, NY 10021
    Proc Natl Acad Sci U S A 87:9712-6. 1990
    The synthesis of oligoribonucleotides by DNA primase in the presence of duplex DNA containing the simian virus 40 (SV40) origin of replication was examined...
  62. pmc Simian virus 40 sequences in human lymphoblastoid B-cell lines
    Riccardo Dolcetti
    Divisions of Experimental Oncology, Centro di Riferimento Oncologico, IRCCS, 33081 Aviano Pordenone, Italy
    J Virol 77:1595-7. 2003
    Human Epstein-Barr virus-immortalized lymphoblastoid B-cell lines tested positive by PCR for simian virus 40 (SV40) DNA (22 of 42 cell lines, 52.3%). B lymphocytes or tissues from which B-cell lines derived were also SV40 positive...
  63. pmc Simian virus 40 induces lamin A/C fluctuations and nuclear envelope deformation during cell entry
    Veronika Butin-Israeli
    Department of Hematology, Hebrew University Hadassah Medical School, Jerusalem, Israel
    Nucleus 2:320-30. 2011
    ..As SV40 serves as a paradigm for the pathogenic human BK, JC and Merkel cell polyomavirus, this study suggests nuclear entry as a novel drug target for these infections...
  64. pmc Calcium bridge triggers capsid disassembly in the cell entry process of simian virus 40
    Masa aki Kawano
    Department of Molecular and Cellular Biology, University of California, Davis, California 95616, USA
    J Biol Chem 284:34703-12. 2009
    ..Our data also indicate that calcium depletion-induced SV40 capsid disassembly may occur in the ER region and that this is essential for successful SV40 infection...
  65. ncbi Immortalization of bovine dental papilla cells with simian virus 40 large t antigen
    B Thonemann
    Department of Operative Dentistry and Periodontology, University of Regensburg, 93042 Regensburg, Germany
    Arch Oral Biol 45:857-69. 2000
    ..pretreatment of the dental papilla were transfected by electroporation with pSV3neo, coding for the oncogene simian virus 40 large t antigen and a neomycin-resistance gene...
  66. pmc Expression of a gonadotropin-releasing hormone receptor-simian virus 40 T-antigen transgene has sex-specific effects on the reproductive axis
    Kyeong Hoon Jeong
    Harvard Medical School, Brigham and Women s Hospital, Division of Endocrinology, Diabetes and Hypertension, 221 Longwood Avenue, Boston, Massachusetts 02115, USA
    Endocrinology 150:3383-91. 2009
    ..The 1.2-kb gene promoter fused to the simian virus 40 large T antigen (TAg) was used to generate transgenic mice that form gonadotrope-derived pituitary tumors at ..
  67. ncbi JCV/BKV and SV40 viral load in lymphoid tissues of young immunocompetent children from an area of north-east Italy
    Manola Comar
    Department of Public Medicine Sciences, UCO Hygiene and Preventive Medicine, University of Trieste, Trieste, Italy
    J Med Virol 82:1236-40. 2010
    ..Nevertheless, the low prevalence of polyomavirus, in comparison with the lymphotropic HHV-6, suggests that these tissues are unlikely to be the preferred site of polyomavirus latency, at least in younger children...
  68. ncbi A role of the TATA box and the general co-activator hTAF(II)130/135 in promoter-specific trans-activation by simian virus 40 small t antigen
    Mona Johannessen
    Department of Biochemistry, Section for Molecular Genetics, Institute of Medical Biology, University of Tromsø, N 9037 Tromsø, Norway
    J Gen Virol 84:1887-97. 2003
    The small t antigen (st-ag) of simian virus 40 can exert pleiotropic effects on biological processes such as DNA replication, cell cycle progression and gene expression...
  69. ncbi Simian virus 40 induces multiple S phases with the majority of viral DNA replication in the G2 and second S phase in CV-1 cells
    J M Lehman
    Department of Microbiology, Immunology, and Molecular Genetics, Center for Immunology and Microbial Diseases, Albany Medical College, New York 12208, USA
    Exp Cell Res 258:215-22. 2000
    The infection of permissive monkey kidney cells (CV-1) with simian virus 40 induces G1 growth-arrested cells into the cell cycle...
  70. pmc Mutations in the GM1 binding site of simian virus 40 VP1 alter receptor usage and cell tropism
    Thomas G Magaldi
    Department of Genetics, Yale School of Medicine, New Haven, Connecticut, USA
    J Virol 86:7028-42. 2012
    ..receptor specificity and how changes in receptor usage affect tropism, we studied the primate polyomavirus simian virus 40 (SV40), which uses the ganglioside GM1 as a receptor that mediates cell binding and entry...
  71. pmc Simian virus 40 transformation, malignant mesothelioma and brain tumors
    Fang Qi
    University of Hawaii Cancer Center, Honolulu, HI, USA
    Expert Rev Respir Med 5:683-97. 2011
    b>Simian virus 40 (SV40) is a DNA virus isolated in 1960 from contaminated polio vaccines, that induces mesotheliomas, lymphomas, brain and bone tumors, and sarcomas, including osteosarcomas, in hamsters...
  72. pmc Importance of Vp1 calcium-binding residues in assembly, cell entry, and nuclear entry of simian virus 40
    Peggy P Li
    Department of Molecular, Cell and Developmental Biology and Molecular Biology Institute, University of California at Los Angeles, Los Angeles, California 90095, USA
    J Virol 77:7527-38. 2003
    ..To define the role of calcium ions in the life cycle of the virus, we analyzed simian virus 40 (SV40) mutants in which structurally deduced calcium-binding amino acids of Vp1 were mutated singly and in ..
  73. ncbi Local actin polymerization and dynamin recruitment in SV40-induced internalization of caveolae
    Lucas Pelkmans
    Swiss Federal Institute of Technology Zurich ETHZ, HPM1 Building, ETH Honggerberg, CH 8093 Zurich, Switzerland
    Science 296:535-9. 2002
    b>Simian virus 40 (SV40) utilizes endocytosis through caveolae for infectious entry into host cells. We found that after binding to caveolae, virus particles induced transient breakdown of actin stress fibers...
  74. ncbi Molecular pathogenesis of malignant mesothelioma and its relationship to simian virus 40
    Adi F Gazdar
    Hamon Center for Therapeutic Oncology Research and Department of Pathology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    Clin Lung Cancer 5:177-81. 2003
    ..The molecular changes caused by each of these major etiologic factors and their interrelationships are the focus of this review...
  75. pmc Enforced expression of Simian virus 40 large T-antigen leads to testicular germ cell tumors in zebrafish
    James A Gill
    Genetics Branch, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland 20889, USA
    Zebrafish 7:333-41. 2010
    ..promoter, which has been shown to enforce high-level expression in the testes of transgenic mice, to express Simian virus 40 large T-antigen in zebrafish testes...
  76. pmc Patterns of polyomavirus SV40 infections and associated cancers in humans: a model
    Janet S Butel
    Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX 77030, USA
    Curr Opin Virol 2:508-14. 2012
    ..This model can explain inconsistencies in the published literature of SV40 infections in humans and can guide the design of future studies...
  77. ncbi Parafibromin tumor suppressor enhances cell growth in the cells expressing SV40 large T antigen
    T Iwata
    Department of Medical Pharmacology, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima, Japan
    Oncogene 26:6176-83. 2007
    ..The tumor suppressor protein PF acts as a positive regulator of cell growth similar to an oncoprotein in the presence of SV40 large T antigen...
  78. ncbi SV40, growth factors, and mesothelioma: another piece of the puzzle
    Brooke T Mossman
    Departments of Pathology and Medicine, University of Vermont College of Medicine, Burlington, VT 05405, USA
    Am J Respir Cell Mol Biol 26:167-70. 2002
  79. ncbi Investigation of the prevalence of antibodies against neurotropic polyomaviruses BK, JC and SV40 in sera from patients affected by multiple sclerosis
    Tatiana Ribeiro
    INSERM U618 Proteases et Vectorisation Pulmonaires, Tours, University Francois Rabelais, Tours, France
    Neurol Sci 31:517-21. 2010
    ....
  80. ncbi The carboxyl-terminal domain of large T antigen rescues SV40 host range activity in trans independent of acetylation
    Danielle L Poulin
    Dana Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA
    Virology 349:212-21. 2006
    ..The carboxyl-terminal region of T Ag contains a biological function essential for the SV40 viral life cycle...
  81. ncbi Simian virus 40 large T-antigen G-quadruplex DNA helicase inhibition by G-quadruplex DNA-interactive agents
    Bodin Tuesuwan
    Division of Medicinal Chemistry, College of Pharmacy, The University of Texas at Austin, Austin, Texas 78712, USA
    Biochemistry 47:1896-909. 2008
    ..The identification of potent and selective inhibitors of the G-quadruplex helicase activity of T-ag provides tools for probing the specific role of this activity in SV40 replication...
  82. pmc Bidirectional DNA unwinding by a ternary complex of T antigen, nucleolin and topoisomerase I
    Stephanie Seinsoth
    Medizinische Biochemie und Molekularbiologie, Universitat des Saarlandes, Gebaude 45, D 66421 Homburg, Germany
    EMBO Rep 4:263-8. 2003
    The simian virus 40 large tumour-antigen (T antigen) DNA helicase is a hexameric structure; it has been proposed that, in viral DNA replication, two of these hexamers are combined to form a bipartite holoenzyme that acts concurrently at ..
  83. ncbi Simian virus 40 VP1 capsid protein forms polymorphic assemblies in vitro
    Shin nosuke Kanesashi
    Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta, Midori ku, Yokohama 226 8503, Japan
    J Gen Virol 84:1899-905. 2003
    The simian virus 40 (SV40) capsid is composed of 72 pentamers of VP1, the major protein of SV40...
  84. pmc Pairs of Vp1 cysteine residues essential for simian virus 40 infection
    Peggy P Li
    Molecular Biology Institute, 456 Boyer Hall, University of California at Los Angeles, 611 East Charles E Young Dr, Box 951570, Los Angeles, CA 90095 1570, USA
    J Virol 79:3859-64. 2005
    Transient disulfide bonding occurs during the intracellular folding and pentamerization of simian virus 40 (SV40) major capsid protein Vp1 (P. P. Li, A. Nakanishi, S. W. Clark, and H. Kasamatsu, Proc. Natl. Acad. Sci...
  85. pmc Gangliosides are receptors for murine polyoma virus and SV40
    Billy Tsai
    Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    EMBO J 22:4346-55. 2003
    Polyoma virus (Py) and simian virus 40 (SV40) travel from the plasma membrane to the endoplasmic reticulum (ER) from where they enter the cytosol and then the nucleus to initiate infection...
  86. pmc Mutagenesis of a functional chimeric gene in yeast identifies mutations in the simian virus 40 large T antigen J domain
    Sheara W Fewell
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA
    Proc Natl Acad Sci U S A 99:2002-7. 2002
    b>Simian virus 40 large T antigen contains an amino terminal J domain that catalyzes T antigen-mediated viral DNA replication and cellular transformation...
  87. pmc 19S late mRNAs of simian virus 40 have an internal ribosome entry site upstream of the virion structural protein 3 coding sequence
    Yongjun Yu
    Department of Cancer Biology, Abramson Family Cancer Research Institute, 314 Biomedical Research Building, 421 Curie Blvd, University of Pennsylvania, Philadelphia, PA 19104 6142, USA
    J Virol 80:6553-8. 2006
    The late mRNAs of simian virus 40 (SV40) are polycistronic. The 19S mRNAs encode primarily the virion structural proteins VP2 and VP3...
  88. ncbi CD4+ T lymphocytes play a critical role in antibody production and tumor immunity against simian virus 40 large tumor antigen
    Ronald C Kennedy
    Department of Microbiology and Immunology, Texas Tech University Health Science Center, Lubbock, Texas 79430, USA
    Cancer Res 63:1040-5. 2003
    ..Our results suggest that CD4+ T cells, along with antibody responses, play a role in the induction of tumor immunity to a viral-encoded tumor antigen...
  89. ncbi Mammalian DNA topoisomerase I activity and poisoning by camptothecin are inhibited by simian virus 40 large T antigen
    Y Pommier
    Laboratory of Molecular Pharmacology, National Cancer Institute, Bethesda, Maryland 20892 4255, USA
    Biochemistry 37:3818-23. 1998
    ..These results demonstrate a functional interaction between T-Ag and top1: they also suggest the importance of top1-protein interactions for the regulation of DNA replication and modulation of camptothecin activity...
  90. pmc Productive simian virus 40 infection of neurons in immunosuppressed Rhesus monkeys
    Xin Dang
    Division of Viral Pathogenesis, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02215, USA
    J Neuropathol Exp Neurol 67:784-92. 2008
    ..Reactivation of simian virus 40 (SV40) in immunosuppressed rhesus monkeys, however, rarely causes a PML-like illness...
  91. pmc Development of quantitative and high-throughput assays of polyomavirus and papillomavirus DNA replication
    Amelie Fradet-Turcotte
    Laboratory of Molecular Virology, Institut de recherches cliniques de Montreal IRCM, Montreal, Quebec, Canada
    Virology 399:65-76. 2010
    ..These quantitative and high-throughput assays should greatly facilitate the study of SV40 and HPV31 DNA replication and the identification of small-molecule inhibitors of this process...
  92. ncbi Immortalization of swine umbilical vein endothelial cells (SUVECs) with the simian virus 40 large-T antigen
    Marcin Chruściel
    Department of Hormonal Action Mechanisms, Institute of Animal Reproduction and Food Research, Polish Academy of Science, Olsztyn, Poland
    Mol Reprod Dev 78:597-610. 2011
    ..We have generated an immortalized endothelial cell line, G-1410, using Simian virus 40 T-antigen (SV40 T-ag) primarily to overcome the short life span before the onset of senescence and high ..
  93. ncbi Bucillamine induces the synthesis of vascular endothelial growth factor dose-dependently in systemic sclerosis fibroblasts via nuclear factor-kappaB and simian virus 40 promoter factor 1 pathways
    Jörg H W Distler
    Center of Experimental Rheumatology, Department of Rheumatology, University Hospital Zurich, Zurich, Switzerland
    Mol Pharmacol 65:389-99. 2004
    ..The study provides evidence for a role of NF-kappaB in the transcriptional regulation of the VEGF gene. SA 96 might have positive aspects on the impaired angiogenesis in patients with SSc...
  94. pmc Disassembly of simian virus 40 during passage through the endoplasmic reticulum and in the cytoplasm
    Dmitry Kuksin
    Department of Microbiology, University of Massachusetts, Amherst, Massachusetts, USA
    J Virol 86:1555-62. 2012
    The nonenveloped polyomavirus simian virus 40 (SV40) is taken up into cells by a caveola-mediated endocytic process that delivers the virus to the endoplasmic reticulum (ER)...
  95. ncbi Some oral poliovirus vaccines were contaminated with infectious SV40 after 1961
    Rochelle Cutrone
    Thoracic Oncology Program, Cardinal Bernardin Cancer Center, Loyola University, Chicago, Illinois, USA
    Cancer Res 65:10273-9. 2005
    ..Moreover, our results indicate possible geographic differences in SV40 exposure and offer a possible explanation for the different percentage of SV40-positive tumors detected in some laboratories...
  96. pmc Intestinal dysplasia induced by simian virus 40 T antigen is independent of p53
    Jennifer A Markovics
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA
    J Virol 79:7492-502. 2005
    Transgenic mice expressing simian virus 40 large T antigen in enterocytes develop intestinal hyperplasia that progresses to dysplasia with age...
  97. pmc Mutational analysis of simian virus 40 T antigen: isolation and characterization of mutants with deletions in the T-antigen gene
    J M Pipas
    Mol Cell Biol 3:203-13. 1983
    A series of mutants of simian virus 40 has been constructed with deletions in the coding sequence for large T antigen. Nucleotide sequence analysis indicates that 4 mutants have in-phase and 11 have out-of-phase deletions...
  98. ncbi The relationship between simian virus 40 and mesothelioma
    Zeyana Rivera
    Cancer Research Center of Hawaii and Department of Pathology, University of Hawaii, Honolulu, Hawaii, USA
    Curr Opin Pulm Med 14:316-21. 2008
    b>Simian virus 40 is present in some human malignant mesotheliomas. The evidence in favor and against a pathogenic role of simian virus 40 in malignant mesothelioma is discussed in this review.
  99. ncbi The binding of topoisomerase I to T antigen enhances the synthesis of RNA-DNA primers during simian virus 40 DNA replication
    Sujata Khopde
    Department of Biological Sciences, University of Delaware, Newark, Delaware 19716 2590, USA
    Biochemistry 47:9653-60. 2008
    Topoisomerase I (topo I) is required for the proper initiation of simian virus 40 (SV40) DNA replication...
  100. ncbi In vitro assessment of the cytotoxicity of nisin, pediocin, and selected colicins on simian virus 40-transfected human colon and Vero monkey kidney cells with trypan blue staining viability assays
    S E Murinda
    Department of Food Science, Pennsylvania State University, University Park, Pennsylvania 16802, USA
    J Food Prot 66:847-53. 2003
    ..bacteriocins (colicins [Col] E1, E3, E6, E7, and K) were evaluated for cytotoxicity against cultured simian virus 40-transfected human colon (SV40-HC) and Vero monkey kidney (Vero) cells...
  101. ncbi High levels of luteinizing hormone analog stimulate gonadal and adrenal tumorigenesis in mice transgenic for the mouse inhibin-alpha-subunit promoter/Simian virus 40 T-antigen fusion gene
    Maarit Mikola
    Department of Physiology, University of Turku, FIN 20520 Turku, Finland
    Oncogene 22:3269-78. 2003
    Transgenic (TG) mice expressing the Simian virus 40 T-antigen under the control of the murine inhibin-alpha promoter (Inhalpha/Tag) develop granulosa and Leydig cell tumors at the age of 5-6 months, with 100% penetrance...

Research Grants63

  1. Screens for host DNA replication and repair factors involved in viral infection
    Matthew D Weitzman; Fiscal Year: 2010
    ..We propose to screen four different DNA viruses (adenovirus, herpesvirus, adeno-associated virus and simian virus 40) against a library of small interfering RNAs targeted to cellular proteins involved in DNA replication, ..
  2. T-antigen Binding to the Merkel Cell Carcinoma Virus Origin
    ALEX ANDREW BOHM; Fiscal Year: 2010
    ..is significantly different from that of better studied family members such as murine polyomavirus and simian virus 40 (SV40)...
  3. CONTROL OF SIMIAN VIRUS 40 AND CELLULAR DNA REPLICATION
    KATHERINE LOUISE FRIEDMAN; Fiscal Year: 2013
    ..Analysis of simian virus 40 (SV40) DNA replication provides the first insight into a eukaryotic primosome that can be reconstituted with ..
  4. Expression of Simiam Virus 40 T Antigens in Intestine
    James M Pipas; Fiscal Year: 2013
    Project Summary/Abstract Simian virus 40 (SV40) encodes a powerful oncoprotein, large T antigen, that is capable of inducing tumors in animals and transforming cells in culture...
  5. Pathogenesis of mesothelioma
    Michele Carbone; Fiscal Year: 2010
    ..Cigarette smoke has been ruled out as a co-factor in MM. Instead, both genetic predisposition and simian virus 40 infections (SV40) have been linked to MM pathogenesis...
  6. Simian Virus 40 (SV40) uncoating and penetration
    Daniel Hebert; Fiscal Year: 2009
    ..goal of the proposal is to elucidate the mechanism of viral uncoating for nonenveloped DNA viruses using Simian Virus 40 (SV40) as a model virus. Viruses replicate within living cells by exploiting the host cellular machinery...
  7. Role of microRNAs in the SV40 infectious cycle
    Christopher S Sullivan; Fiscal Year: 2012
    ..Recent work from our laboratory has demonstrated that the miRNAs encoded by Simian Virus 40 (SVmiRNA) function to provide a growth advantage to infected cells undergoing an interferon response...
  8. HTLV-III SEQUENCES AT THE TRANSCRIPTIONAL LEVEL
    JOSEPH MOSCA; Fiscal Year: 1991
    ....
  9. MECHANISMS OF NUCLEAR PROTEIN ACCUMULATION
    PHILIP PAINE; Fiscal Year: 1993
    ..study the nucleocytoplasmic transport of (i) two widely-studied prototype N- proteins, nucleoplasmic and the simian virus 40 large T antigen (SV40 lg T), and (ii) macromolecular dextrans with covalently attached signal sequences of ..
  10. Hyperacetylation of Biologically Active SV40 Chromatin
    Barry Milavetz; Fiscal Year: 2006
    unreadable] DESCRIPTION (provided by applicant): Using simian virus 40 (SV40) as a model for infections by DNA viruses, the proposed research will contribute to our understanding of the relationship between chromatin remodeling through ..
  11. HPV DNA REPLICATION: NOVEL HOST FACTORS
    Thomas Melendy; Fiscal Year: 2003
    ..Bruce Stillman studying simian virus 40 (SV40) DNA replication factors and mechanisms. Dr...
  12. AIDS-assoc Progressive Multifocal Leukoencephalopathy
    Igor Koralnik; Fiscal Year: 2004
    ..Due to the narrow host range of JCV, there is no adequate animal model for its study. The Simian Virus 40 (SV40) has 69% homology with JCV, and has been associated with a PML like disease in immunosuppressed monkeys...
  13. EARLY INTERACTIONS OF DNA VIRUSES AND HOST
    Gerd Maul; Fiscal Year: 2005
    ..We mapped the targeting DNA sequence for simian virus 40 (SV40) to the core origin of replication. Also DNA viruses begin to transcribe in association with ND10...
  14. HISTONE HYPERACETYLATION IN REPLICATING SV40 CHROMOSOMES
    Barry Milavetz; Fiscal Year: 2009
    Using the Simian Virus 40 (SV40) chromosome as a model for eukaryotic chromatin, the proposed research will address the role of histone hyperacetylation at an active replication fork and during post-replication chromatin maturation ..
  15. MOLECULAR BIOLOGY OF CAMPTOTHECIN INDUCED DNA DAMAGE
    ROBERT SNAPKA; Fiscal Year: 2002
    ..to our understanding of the cytotoxic mechanisms of camptothecins have been made with studies of simian virus 40 (SV40), a small double strand DNA virus which makes such extensive use of host cell chromosomal proteins and ..
  16. REGULATION OF CELLULAR FUNCTIONS BY DNA TUMOR VIRUSES
    JAMES ALWINE; Fiscal Year: 2009
    DESCRIPTION (provided by applicant): Simian virus 40 (SV40) and human cytomegalovirus (HCMV) activate the cell's metabolic and protein synthetic environment to support viral replication...
  17. TRANSFORMING DOMAINS IN THE IGF-1 RECEPTOR
    Christian Sell; Fiscal Year: 1999
    ..in serum free medium supplemented with PDGF, EGF, IGF-1, and cannot be transformed by the introduction of the simian virus 40 (SV4O) large T antigen...
  18. PATHOLOGY OF NEOPLASTIC TRANSFORMATION
    JOHN LEHMAN; Fiscal Year: 1990
    ..has been collected utilizing various cell model systems infected with the oncogenic DNA tumor virus, Simian virus 40 (SV40)...
  19. Selection of chemical inhibitors of oncoproteins
    Maurizio Bocchetta; Fiscal Year: 2002
    ..A growing body of evidence relates Simian Virus 40 (SV40) with tumors of the mesothelium, brain, and bone...
  20. A NOVEL FUNCTION OF THE SIMIAN VIRUS 40 GENOME
    Dana Carroll; Fiscal Year: 1980
    Examination of a wide variety of Simian Virus 40 mutant and defective genomes shows that, with one possible exception, they all have retained, in addition to the replication origin at map position 0...
  21. MECHANISMS OF EUCARYOTIC TRANSCRIPTIONAL ACTIVATION
    JAMES ALWINE; Fiscal Year: 1990
    ..We have previously identified an element in the late promoter of a DNA virus, simian virus 40 (SV40), which responds to transactivation mediated by the early viral protein T antigen...
  22. Deregulation of Cell Pathways by Ad E1B 55K Oncoproteins
    Daiqing Liao; Fiscal Year: 2005
    ..For example, several viral oncogenes, such as the simian virus 40 large-T antigen, the human papillomavirusl6 E6 and E7 oncoproteins, and the adenovirus (Ad) E1A and E1B ..
  23. IMMUNIZATION AGAINST LENTIVIRAL GP120 USING SV40 VECTORS
    David Strayer; Fiscal Year: 2000
    ....
  24. IMMORTALIZED CELLS FOR CYSTIC FIBROSIS RESEARCH
    James Yankaskas; Fiscal Year: 1992
    ..We will utilize the immortalizing genes of Simian Virus 40 for these studies, based on its record of producing human epithelial cell lines with differentiated ..
  25. PRODUCTION OF SV40-SPECIFIED ANTIGENS IN E COLI
    Geoffrey Wilson; Fiscal Year: 1980
    b>Simian virus 40 (SV40) is a small oncogenic mammalian virus...
  26. Modulators of T Antigen-Mediated Chaperone Action
    JEFFREY BRODSKY; Fiscal Year: 2004
    DESCRIPTION (provided by applicant)Members of the oncogenic Polyomavirus family, such as Simian Virus 40 (SV40) and the human BK and JC viruses, require only one virally-encoded protein to transform mammalian cells: T antigen...
  27. T CELL FOCUSED CANCER VACCINES IN MURINE TUMOR MODELS
    ROBERT BRIGHT; Fiscal Year: 2002
    ..Recent studies have described the presence of simian virus 40 (SV40)-like gene sequences and proteins (specifically the large tumor antigen: SV40 Tag) in human ..
  28. SV40 st induced cell transformation via PP2A inhibition
    Madathia Sarkissian; Fiscal Year: 2007
    ..has been to examine the molecular details of transformation of cultured cells via the early region of the simian virus 40 viral genome...
  29. ABERRANT PAPOVAVIRUS REPLICATION AFTER GENOTOXIC DAMAGE
    ROBERT SNAPKA; Fiscal Year: 1991
    b>Simian virus 40 (SV40) will be used as a model cellular replicon in the study of both normal and aberrant DNA replication in mammalian cells...
  30. Role of SV40 in Salivary Gland Tumorigenesis
    LESLEY ELLIES; Fiscal Year: 2003
    ..to understand early molecular changes occurring in salivary gland tumorigenesis; 2) to determine the role of simian virus 40 (SV40) small t antigen (t-ag) in salivary gland tumorigenesis; and 3) to create an animal model which can be ..
  31. STRUCTURE OF NATIVE MAMMALIAN TRANSCRIPTION COMPLEXES
    LEONARD LUTTER; Fiscal Year: 2000
    ..Native transcription complexes from transfected plasmid DNA as well as from simian virus 40 will be investigated. Two general aspects will be studied...
  32. CYTOMEGALOVIRUS-INDUCED SUPPRESSION OF TUMOR IMMUNITY
    Ann Campbell; Fiscal Year: 1990
    ..proposes to utilize the knowledge and techniques of the well-characterized cell-mediated response to the simian virus 40 (SV40) tumor rejection antigen as a means of defining alterations in cell-mediated immunity during an acute ..
  33. REGULATION OF AAV DNA REPLICATION
    KENNETH BERNS; Fiscal Year: 2000
    A hybrid adeno-associated virus (AAV)/simian virus 40 (SV40) genome has been constructed by insertion of the SV40 regulatory region (nt 5171-5243-270) into a deletion in the AAV genome from nt 144-264...
  34. NEGATIVE REGULATION OF AAV DNA REPLICATION
    KENNETH BERNS; Fiscal Year: 1992
    A hybrid adeno-associated virus (AAV)/simian virus 40 (SV40) genome has been constructed by insertion of the SV40 regulatory region (nt 5171-5243-270) into a deletion in the AAV genome from nt 144-264...
  35. STUDIES OF THE IN VITRO HEMATOPOIETIC MICROENVIRONMENT
    Jack Singer; Fiscal Year: 1991
    ..studies are in part possible due to the development of a new technique for immortalizing these cells using simian virus 40. With this method, clonable microenvironmental cell lines can be produced from most patients and the origin, ..
  36. VIRAL CARCINOGENESIS WITH SPECIAL REFERENCE TO SV40
    GEORGE DIAMANDOPOULOS; Fiscal Year: 1980
    ..studies is the continuation of research on various aspects of viral carcinogenesis with special reference to simian virus 40 (SV40). This undertaking has been pursued by the Principal Investigator for the past 16 years (1963-1979)...
  37. INHIBITION OF DNA REPLICATION BY DNA DAMAGING DRUGS
    Terry Beerman; Fiscal Year: 2001
    ..The approach utilizes well-defined intracellular Simian Virus 40 and Epstein-Barr Virus DNA to determine whether inhibition of DNA replication by a limited number of DNA-..
  38. THE PROCESS AND CONTROL OF TRANSCRIPTION OF SV40
    MELVIN GREEN; Fiscal Year: 1980
    Our primary objective is to gain a thorough understanding of the relation of simian virus 40 (SV40) chromatin structure to its function as a template for transcription and replication...
  39. SV40 as a cardiac gene therapy vector
    Ronen Beeri; Fiscal Year: 2004
    ..The SV40 (simian virus 40) pseudovirion shows promise as a persisting, non inflammation-inducing vector...
  40. ENZYMOLOGY OF SV40 DNA REPLICATION: IN VITRO STUDIES
    Howard Edenberg; Fiscal Year: 1980
    ..To determine which enzymes are required for DNA replication, I propose to analyze the replication in vitro of simian virus 40 (SV40) chromosomes...
  41. SV40 Pathogenesis of Human Infections
    JANET BUTEL; Fiscal Year: 2008
    ..This new information will allow better understanding of the biology of SV40 human infections and may provide insights into the mechanisms of SV40 involvement in human malignancies. [unreadable] [unreadable]..
  42. Hamster Model for AIDS Lymphomas
    JANET BUTEL; Fiscal Year: 2003
    ..In addition, this project may provide new insights into lymphomagenesis in general and may lead to the development of new diagnostic and therapeutic approaches to this malignancy. ..
  43. HPV ETIOLOGY OF A SUBSET OF HEAD AND NECK CANCERS
    Keerti Shah; Fiscal Year: 2004
    ..If HPV etiology is established for a subset of HNSCC, patients with these tumors may benefit from HPV-specific immunotherapies which are being evaluated for the treatment of invasive cervical cancer. ..
  44. Genetics of SV40 Entry and Minichromosome Transport
    LEONARD NORKIN; Fiscal Year: 2008
    DESCRIPTION (provided by applicant): Simian virus 40 (SV40) infects cells by binding to plasma membrane MHC class I molecules and then entering via a unique mechanism mediated by caveolae, rather than clathrin-coated pits...
  45. CSHL Symposium on Quantitative Biology
    Bruce Stillman; Fiscal Year: 2006
    ..The meeting will address the phenomenon of small ribonucleic acids and their function in a variety of regulatory phenomena in the cell. [unreadable] [unreadable] [unreadable]..
  46. REPLICATION OF DAMAGED DNA IN HUMAN CELLS
    Marila Cordeiro Stone; Fiscal Year: 2004
    ..This project will provide insight on how PRR and the S checkpoint cooperate to protect human cells from cancer. ..
  47. EUKARYOTIC DNA REPLICATION CONFERENCE
    Bruce Stillman; Fiscal Year: 2005
    ..The subsequent meetings (2003 and 2005) will follow a similar format which will include topics that are highly relevant to the current research at the time of the meeting. ..
  48. Structure and Function of SV40 Large Tumor Antigen
    DANIEL SIMMONS; Fiscal Year: 2009
    ..abstract_text> ..
  49. Transcriptional Regulation of a Surfactant Enzyme
    RAMA MALLAMPALLI; Fiscal Year: 2003
    ..Ultimately, the results from these studies could lead to therapeutic strategies directed at modulating expression of this key surfactant enzyme at the molecular level. ..
  50. CSHL Symposium on Quantitative Biology
    Bruce Stillman; Fiscal Year: 2004
    ..The meeting will address the molecular basis of epigenetic phenomena and their relationship to chromatin and chromosome structure and function. ..
  51. Conference--CSHL on Quantitative Biology
    Bruce Stillman; Fiscal Year: 2003
    ..The annual Symposia will continue to be planned to further the progress of advancements in biomedical science. This proposal requests support for 2003 to cover the 68th Cold Spring Harbor Symposium. ..
  52. COLD SPRING HARBOR SYMPOSIUM ON QUANTITATIVE BIOLOGY
    Bruce Stillman; Fiscal Year: 2005
    ..The annual Symposia will continue to be planned to further the progress of advancements in biomedical science. ..
  53. The Role of Polyomaviruses in Pediatric Renal Diseases
    JOHN VANCHIERE; Fiscal Year: 2006
    ..They also will provide me a firm foundation on which to build an academic career. Dr. Butel's laboratory provides an excellent environment in which to develop my scientific skills. ..
  54. Role of Fra-1 in Mesothelioma
    Maria Ramos Nino; Fiscal Year: 2008
    ..an aggressive malignancy associated with occupational exposure to asbestos and more recently linked to Simian Virus 40 (SV40) is a fatal tumor resistant to therapy...
  55. Signals Encoded in DNA that Affect Chromatin Structure
    Arnold Stein; Fiscal Year: 2003
    ..Information obtained here might ultimately enable one to predict functionally important aspects of chromatin structure computationally from DNA sequence data. ..
  56. PATHOGENESIS OF MALIGNANT FIBROMATOSIS
    David Strayer; Fiscal Year: 2001
    ..These studies will help elucidate the mechanisms by which viruses may manipulate and subvert cellular survival and death mechanisms to replicate themselves, suppress immune function, and cause tumors. ..
  57. PROTECTING CNS CELLS FROM HIV AND HIV-INDUCED INJURY
    David Strayer; Fiscal Year: 2004
    ..We propose to address this therapeutic challenge using rSV40 gene delivery to the CNS, both to protect the brain from HIV infection and to mitigate HIV-induced CNS dysfunction. ..
  58. Role of Cell Mediators in Asbestos-SV40 Carcinogenesis
    Michele Carbone; Fiscal Year: 2009
    ..It is anticipated that the results of the experimens proposed in this application will provide information to design novel preventive and therapeutic approaches for MM. ..
  59. Contrasting Properties of Integrin Cytoplasmic Domains
    Renata Pasqualini; Fiscal Year: 2004
    ..A better understanding of angiogenesis and new ways of manipulating such process in physiological and pathological situations may result from this work. ..
  60. Targeting HIV infection of the cns using gene delivery
    David Strayer; Fiscal Year: 2008
    ..We propose to address this therapeutic challenge using gene delivery to the CNS, both to protect the brain from HIV infection and to mitigate HIV-induced CNS dysfunction. ..
  61. P38, G2 Arrest, and Temozolomide Resistance in Gliomas
    RUSSELL PIEPER; Fiscal Year: 2008
    ....
  62. SV40 T Antigen Role in Virus Growth and Transformation
    James M Pipas; Fiscal Year: 2010
    ..DNA tumor viruses, such as simian virus 40 (SV40) have proved to be powerful tools for uncovering pathways that mediate cell proliferation, ..