transgenic mice

Summary

Summary: Laboratory mice that have been produced from a genetically manipulated EGG or EMBRYO.

Top Publications

  1. pmc Cre reporter strains produced by targeted insertion of EYFP and ECFP into the ROSA26 locus
    S Srinivas
    Department of Genetics and Development, Columbia University, New York, USA
    BMC Dev Biol 1:4. 2001
  2. ncbi Alzheimer's disease is a synaptic failure
    Dennis J Selkoe
    Center for Neurologic Diseases, Brigham and Women s Hospital, and the Harvard Center for Neurodegeneration and Repair, Boston, MA 02115, USA
    Science 298:789-91. 2002
  3. ncbi Motor neuron degeneration in mice that express a human Cu,Zn superoxide dismutase mutation
    M E Gurney
    Department of Cell and Molecular Biology, Northwestern University Medical School, Chicago, IL 60611
    Science 264:1772-5. 1994
  4. ncbi Triple-transgenic model of Alzheimer's disease with plaques and tangles: intracellular Abeta and synaptic dysfunction
    Salvatore Oddo
    Department of Neurobiology and Behavior, University of California, Irvine, Irvine, California 92697, USA
    Neuron 39:409-21. 2003
  5. ncbi A gene expression atlas of the central nervous system based on bacterial artificial chromosomes
    Shiaoching Gong
    GENSAT Project, Howard Hughes Medical Institute, The Rockefeller University, 1230 York Avenue, Box 260, New York 10021, USA
    Nature 425:917-25. 2003
  6. pmc In vivo analysis of autophagy in response to nutrient starvation using transgenic mice expressing a fluorescent autophagosome marker
    Noboru Mizushima
    Department of Cell Biology, National Institute for Basic Biology, Okazaki 444 8585, Japan
    Mol Biol Cell 15:1101-11. 2004
  7. ncbi A novel transcription factor, T-bet, directs Th1 lineage commitment
    S J Szabo
    Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, Massachusetts 02115, USA
    Cell 100:655-69. 2000
  8. ncbi Control of regulatory T cell development by the transcription factor Foxp3
    Shohei Hori
    Laboratory of Immunopathology, Research Center for Allergy and Immunology, Institute for Physical and Chemical Research, Yokohama 230 0045, Japan
    Science 299:1057-61. 2003
  9. ncbi 'Green mice' as a source of ubiquitous green cells
    M Okabe
    Research Institute for Microbial Diseases, Osaka University, Suita, Japan
    FEBS Lett 407:313-9. 1997
  10. ncbi Intraneuronal beta-amyloid aggregates, neurodegeneration, and neuron loss in transgenic mice with five familial Alzheimer's disease mutations: potential factors in amyloid plaque formation
    Holly Oakley
    Department of Cell and Molecular Biology, The Feinberg School of Medicine, Northwestern University, Chicago, Illinois 60611, USA
    J Neurosci 26:10129-40. 2006

Detail Information

Publications342 found, 100 shown here

  1. pmc Cre reporter strains produced by targeted insertion of EYFP and ECFP into the ROSA26 locus
    S Srinivas
    Department of Genetics and Development, Columbia University, New York, USA
    BMC Dev Biol 1:4. 2001
    ....
  2. ncbi Alzheimer's disease is a synaptic failure
    Dennis J Selkoe
    Center for Neurologic Diseases, Brigham and Women s Hospital, and the Harvard Center for Neurodegeneration and Repair, Boston, MA 02115, USA
    Science 298:789-91. 2002
    ....
  3. ncbi Motor neuron degeneration in mice that express a human Cu,Zn superoxide dismutase mutation
    M E Gurney
    Department of Cell and Molecular Biology, Northwestern University Medical School, Chicago, IL 60611
    Science 264:1772-5. 1994
    ..to alanine at position 93--a change that has little effect on enzyme activity--caused motor neuron disease in transgenic mice. The mice became paralyzed in one or more limbs as a result of motor neuron loss from the spinal cord and ..
  4. ncbi Triple-transgenic model of Alzheimer's disease with plaques and tangles: intracellular Abeta and synaptic dysfunction
    Salvatore Oddo
    Department of Neurobiology and Behavior, University of California, Irvine, Irvine, California 92697, USA
    Neuron 39:409-21. 2003
    ....
  5. ncbi A gene expression atlas of the central nervous system based on bacterial artificial chromosomes
    Shiaoching Gong
    GENSAT Project, Howard Hughes Medical Institute, The Rockefeller University, 1230 York Avenue, Box 260, New York 10021, USA
    Nature 425:917-25. 2003
    ..The atlas, library of BAC vectors and BAC transgenic mice generated in this screen provide a rich resource that allows a broad array of investigations not previously ..
  6. pmc In vivo analysis of autophagy in response to nutrient starvation using transgenic mice expressing a fluorescent autophagosome marker
    Noboru Mizushima
    Department of Cell Biology, National Institute for Basic Biology, Okazaki 444 8585, Japan
    Mol Biol Cell 15:1101-11. 2004
    ..To understand where and when autophagy occurs in vivo, we have generated transgenic mice systemically expressing GFP fused to LC3, which is a mammalian homologue of yeast Atg8 (Aut7/Apg8) and serves ..
  7. ncbi A novel transcription factor, T-bet, directs Th1 lineage commitment
    S J Szabo
    Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, Massachusetts 02115, USA
    Cell 100:655-69. 2000
    ..Thus, T-bet initiates Th1 lineage development from naive Thp cells both by activating Th1 genetic programs and by repressing the opposing Th2 programs...
  8. ncbi Control of regulatory T cell development by the transcription factor Foxp3
    Shohei Hori
    Laboratory of Immunopathology, Research Center for Allergy and Immunology, Institute for Physical and Chemical Research, Yokohama 230 0045, Japan
    Science 299:1057-61. 2003
    ..Thus, Foxp3 is a key regulatory gene for the development of regulatory T cells...
  9. ncbi 'Green mice' as a source of ubiquitous green cells
    M Okabe
    Research Institute for Microbial Diseases, Osaka University, Suita, Japan
    FEBS Lett 407:313-9. 1997
    ..The fluorescent nature of the cells from these transgenic mouse lines would facilitate their use in many kinds of cell transplantation experiments...
  10. ncbi Intraneuronal beta-amyloid aggregates, neurodegeneration, and neuron loss in transgenic mice with five familial Alzheimer's disease mutations: potential factors in amyloid plaque formation
    Holly Oakley
    Department of Cell and Molecular Biology, The Feinberg School of Medicine, Northwestern University, Chicago, Illinois 60611, USA
    J Neurosci 26:10129-40. 2006
    ..b>Transgenic mice that express FAD mutant APP and PS1 overproduce Abeta42 and exhibit amyloid plaque pathology similar to that ..
  11. ncbi Disruption of the glucocorticoid receptor gene in the nervous system results in reduced anxiety
    F Tronche
    Molecular Biology of the Cell I, Deutsches Krebsforschungzentrum, Im Neuenheimer Feld 280, 69120 Heidelberg, Germany
    Nat Genet 23:99-103. 1999
    ..Conditional mutagenesis of Gr in the nervous system provides genetic evidence for the importance of Gr signalling in emotional behaviour because mutant animals show an impaired behavioural response to stress and display reduced anxiety...
  12. ncbi Osteoblastic cells regulate the haematopoietic stem cell niche
    L M Calvi
    Endocrine Unit, Department of Medicine, Center for Human Genetics and Molecular Pediatric Disease, University of Rochester School of Medicine, Rochester, New York 14642, USA
    Nature 425:841-6. 2003
    ..Niche constituent cells or signalling pathways provide pharmacological targets with therapeutic potential for stem-cell-based therapies...
  13. ncbi Exon 1 of the HD gene with an expanded CAG repeat is sufficient to cause a progressive neurological phenotype in transgenic mice
    L Mangiarini
    Division of Medical and Molecular Genetics, UMDS, Guy s Hospital, London, United Kingdom
    Cell 87:493-506. 1996
    ..In three lines, the transgene is ubiquitously expressed at both mRNA and protein level. Transgenic mice exhibit a progressive neurological phenotype that exhibits many of the features of HD, including choreiform-..
  14. ncbi Mice deficient for p53 are developmentally normal but susceptible to spontaneous tumours
    L A Donehower
    Division of Molecular Virology, Baylor College of Medicine, Houston, Texas 77030
    Nature 356:215-21. 1992
    ....
  15. ncbi Reducing endogenous tau ameliorates amyloid beta-induced deficits in an Alzheimer's disease mouse model
    Erik D Roberson
    Gladstone Institute of Neurological Disease, San Francisco, CA 94158, USA
    Science 316:750-4. 2007
    ..Here, we found that reducing endogenous tau levels prevented behavioral deficits in transgenic mice expressing human amyloid precursor protein, without altering their high Abeta levels...
  16. pmc Defining the epithelial stem cell niche in skin
    Tudorita Tumbar
    Howard Hughes Medical Institute, Laboratory of Mammalian Cell Biology and Development, Rockefeller University, New York, NY 10021, USA
    Science 303:359-63. 2004
    ..Many of the >100 messenger RNAs preferentially expressed in the niche encode surface receptors and secreted proteins, enabling LRCs to signal and respond to their environment...
  17. ncbi IL-23 and IL-17 in the establishment of protective pulmonary CD4+ T cell responses after vaccination and during Mycobacterium tuberculosis challenge
    Shabaana A Khader
    Trudeau Institute, Saranac Lake, New York 12983, USA
    Nat Immunol 8:369-77. 2007
    ....
  18. ncbi Conditional gene targeting in macrophages and granulocytes using LysMcre mice
    B E Clausen
    Institute for Genetics, University of Cologne, Germany
    Transgenic Res 8:265-77. 1999
    ..In contrast, no significant deletion was observed in tail DNA or purified T and B cells. Taken together, LysMcre mice allow for both specific and highly efficient Cre-mediated deletion of loxP-flanked target genes in myeloid cells...
  19. ncbi Modification of gene activity in mouse embryos in utero by a tamoxifen-inducible form of Cre recombinase
    P S Danielian
    Department of Molecular and Cellular Biology Harvard University 16 Divinity Avenue, Cambridge Massachusetts 02138 USA
    Curr Biol 8:1323-6. 1998
    ..Induction was ligand dependent, rapid and efficient. The results demonstrate that tamoxifen-inducible recombination can be used to effectively modify gene function in the mouse embryo...
  20. pmc Tau suppression in a neurodegenerative mouse model improves memory function
    K SantaCruz
    Department of Laboratory Medicine and Pathology, University of Minnesota Medical School, Minneapolis, MN 55455, USA
    Science 309:476-81. 2005
    ..Thus, NFTs are not sufficient to cause cognitive decline or neuronal death in this model of tauopathy...
  21. ncbi Inducible gene targeting in mice
    R Kuhn
    Institute for Genetics, University of Cologne, Germany
    Science 269:1427-9. 1995
    ..Deletion was complete in liver and nearly complete in lymphocytes within a few days, whereas partial deletion was obtained in other tissues. This method can be used for the inducible inactivation of any other gene in vivo...
  22. pmc TDP-43 mutant transgenic mice develop features of ALS and frontotemporal lobar degeneration
    Iga Wegorzewska
    Department of Neurology and Hope Center for Neurological Diseases, Washington University School of Medicine, St Louis, MO 63110, USA
    Proc Natl Acad Sci U S A 106:18809-14. 2009
    ....
  23. ncbi Onset and progression in inherited ALS determined by motor neurons and microglia
    Severine Boillee
    Ludwig Institute for Cancer Research and Departments of Medicine and Neuroscience, University of California at San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA
    Science 312:1389-92. 2006
    ....
  24. ncbi Lineage relationship and protective immunity of memory CD8 T cell subsets
    E John Wherry
    Emory Vaccine Center and Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, GA 30322, USA
    Nat Immunol 4:225-34. 2003
    ..We propose that T(CM) and T(EM) do not necessarily represent distinct subsets, but are part of a continuum in a linear naive --> effector --> T(EM) --> T(CM) differentiation pathway...
  25. ncbi CD1d-restricted and TCR-mediated activation of valpha14 NKT cells by glycosylceramides
    T Kawano
    CREST Core Research for Evolutional Science and Technology Project, Japan Science and Technology Corporation JST, 1 8 1 Inohana, Chuo, Chiba 260, Japan
    Science 278:1626-9. 1997
    ..Thus, this lymphocyte shares distinct recognition systems with either T or NK cells...
  26. ncbi Selective expression of the interleukin 7 receptor identifies effector CD8 T cells that give rise to long-lived memory cells
    Susan M Kaech
    Emory Vaccine Center and Department of Microbiology and Immunology, Emory University School of Medicine, 1510 Clifton Road, Atlanta, Georgia 30322, USA
    Nat Immunol 4:1191-8. 2003
    ..Thus, selective expression of IL-7R identifies memory cell precursors, and this marker may be useful in predicting the number of memory T cells generated after infection or immunization...
  27. ncbi Self-renewal, multipotency, and the existence of two cell populations within an epithelial stem cell niche
    Cedric Blanpain
    Howard Hughes Medical Institute, The Rockefeller University, New York, NY 10021, USA
    Cell 118:635-48. 2004
    ..These findings suggest that the niche microenvironment imposes intrinsic "stemness" features without restricting the establishment of epithelial polarity and changes in gene expression...
  28. pmc A distinct lineage of CD4 T cells regulates tissue inflammation by producing interleukin 17
    Heon Park
    Department of Immunology, University of Washington, Seattle, Washington 98195, USA
    Nat Immunol 6:1133-41. 2005
    ..Thus, IL-17 expression characterizes a unique T helper lineage that regulates tissue inflammation...
  29. ncbi Immunization with amyloid-beta attenuates Alzheimer-disease-like pathology in the PDAPP mouse
    D Schenk
    Elan Pharmaceuticals, South San Francisco, California 94080, USA
    Nature 400:173-7. 1999
    ..Our results raise the possibility that immunization with amyloid-beta may be effective in preventing and treating Alzheimer's disease...
  30. ncbi Multiple roles for activated LEF/TCF transcription complexes during hair follicle development and differentiation
    R Dasgupta
    Howard Hughes Medical Institute, Department of Molecular Genetics and Cell Biology and Committee on Developmental Biology, The University of Chicago, Chicago, IL 60637, USA
    Development 126:4557-68. 1999
    ..Furthermore, our findings unveil the importance of activated LEF/TCF complexes at distinct times in hair development and cycling when changes in cell fate and differentiation commitments take place...
  31. ncbi Preferential incorporation of adult-generated granule cells into spatial memory networks in the dentate gyrus
    Nohjin Kee
    Program in Neurosciences and Mental Health, The Hospital for Sick Children, 555 University Avenue, Toronto, Ontario M5G 1X8, Canada
    Nat Neurosci 10:355-62. 2007
    ..This preferential recruitment supports the idea that new neurons make a unique contribution to memory processing in the dentate gyrus...
  32. pmc Requirement for hippocampal CA3 NMDA receptors in associative memory recall
    Kazu Nakazawa
    Picower Center for Learning and Memory, RIKEN MIT Neuroscience Research Center, Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Science 297:211-8. 2002
    ..These results provide direct evidence for CA3 NMDA receptor involvement in associative memory recall...
  33. ncbi Loss of CTLA-4 leads to massive lymphoproliferation and fatal multiorgan tissue destruction, revealing a critical negative regulatory role of CTLA-4
    E A Tivol
    Department of Pathology, Brigham and Women s Hospital, Boston, Massachusetts, USA
    Immunity 3:541-7. 1995
    ..In the absence of CTLA-4, peripheral T cells are activated, can spontaneously proliferate, and may mediate lethal tissue injury...
  34. pmc PERIOD2::LUCIFERASE real-time reporting of circadian dynamics reveals persistent circadian oscillations in mouse peripheral tissues
    Seung Hee Yoo
    Department of Neurobiology and Physiology, Northwestern University, 2205 Tech Drive, Evanston, IL 60208, USA
    Proc Natl Acad Sci U S A 101:5339-46. 2004
    ..These results demonstrate that peripheral tissues express self-sustained, rather than damped, circadian oscillations and suggest the existence of organ-specific synchronizers of circadian rhythms at the cell and tissue level...
  35. ncbi Transgenic mice with hematopoietic and lymphoid specific expression of Cre
    Jasper de Boer
    Department of Molecular Immunology, National Institute for Medical Research, The Ridgeway, London, GB
    Eur J Immunol 33:314-25. 2003
    ..Both of these Cre-transgenic lines will be useful in generating tissue-specific gene deletions within all the cells of hematopoietic or lymphoid tissues...
  36. ncbi A mouse Mecp2-null mutation causes neurological symptoms that mimic Rett syndrome
    J Guy
    Wellcome Centre for Cell Biology, Institute of Cell and Molecular Biology, University of Edinburgh, The King s Buildings, Edinburgh, UK
    Nat Genet 27:322-6. 2001
    ....
  37. ncbi Tie2-Cre transgenic mice: a new model for endothelial cell-lineage analysis in vivo
    Y Y Kisanuki
    Department of Molecular Genetics, Howard Hughes Medical Institute, Dallas, Texas, 75390 9050, USA
    Dev Biol 230:230-42. 2001
    ..Here, we created Tie2-Cre transgenic mice, in which expression of Cre recombinase is driven by an endothelial-specific promoter/enhancer...
  38. ncbi Microglia in the adult brain arise from Ly-6ChiCCR2+ monocytes only under defined host conditions
    Alexander Mildner
    Institute of Neuropathology, Georg August University, Gottingen, Germany
    Nat Neurosci 10:1544-53. 2007
    ..Our results identify Ly-6C(hi)CCR2(+) monocytes as direct precursors of microglia in the adult brain and establish the importance of local factors in the adult CNS for microglia engraftment...
  39. ncbi SIRT1 transgenic mice show phenotypes resembling calorie restriction
    Laura Bordone
    Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Aging Cell 6:759-67. 2007
    We generated mice that overexpress the sirtuin, SIRT1. Transgenic mice have been generated by knocking in SIRT1 cDNA into the beta-actin locus...
  40. pmc TDP-43 transgenic mice develop spastic paralysis and neuronal inclusions characteristic of ALS and frontotemporal lobar degeneration
    Hans Wils
    Department of Molecular Genetics, VIB, B 2610 Antwerpen, Belgium
    Proc Natl Acad Sci U S A 107:3858-63. 2010
    ..These findings suggest that approximately 25-kDa TDP-43 CTFs are noxious to neurons by a gain of aberrant nuclear function...
  41. ncbi Proapoptotic Bcl-2 relative Bim required for certain apoptotic responses, leukocyte homeostasis, and to preclude autoimmunity
    P Bouillet
    The Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria 3050, Australia
    Science 286:1735-8. 1999
    ..Thus, Bim is required for hematopoietic homeostasis and as a barrier to autoimmunity. Moreover, particular death stimuli appear to activate apoptosis through distinct BH3-only proteins...
  42. pmc Targeted disruption of the mouse transforming growth factor-beta 1 gene results in multifocal inflammatory disease
    M M Shull
    Department of Molecular Genetics, Biochemistry, and Microbiology, University of Cincinnati College of Medicine, Ohio 45267 0524
    Nature 359:693-9. 1992
    ..TGF-beta 1-deficient mice may be valuable models for human immune and inflammatory disorders, including autoimmune diseases, transplant rejection and graft versus host reactions...
  43. ncbi Bone marrow-derived microglia play a critical role in restricting senile plaque formation in Alzheimer's disease
    Alain R Simard
    Laboratory of Molecular Endocrinology, CHUL Research Center and Department of Anatomy and Physiology, Laval University, 2705 Laurier Boul, Quebec G1V 4G2, Canada
    Neuron 49:489-502. 2006
    ..Therapeutic strategies aiming to improve their recruitment could potentially lead to a new powerful tool for the elimination of toxic senile plaques...
  44. ncbi Efficient recombination in diverse tissues by a tamoxifen-inducible form of Cre: a tool for temporally regulated gene activation/inactivation in the mouse
    Shigemi Hayashi
    Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Avenue, Cambridge, Massachusetts 02138, USA
    Dev Biol 244:305-18. 2002
    ..This inducible Cre system will be a broadly useful tool to modulate gene activity in mouse embryos, adults, and culture systems where temporal control is an important consideration...
  45. pmc Small intestine lamina propria dendritic cells promote de novo generation of Foxp3 T reg cells via retinoic acid
    Cheng Ming Sun
    Mucosal Immunology Unit, Laboratory of Parasitic Diseases, National Institute for Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Exp Med 204:1775-85. 2007
    ..Together, these data demonstrate that the intestinal immune system has evolved a self-contained strategy to promote T reg cell neoconversion...
  46. pmc Altered distributions of Gemini of coiled bodies and mitochondria in motor neurons of TDP-43 transgenic mice
    Xiu Shan
    Department of Pathology, Division of Neuropathology, The Johns Hopkins University School of Medicine, Baltimore, MD 21205 2196, USA
    Proc Natl Acad Sci U S A 107:16325-30. 2010
    ..In concert, our findings indicate that TDP-43 participates in pathways critical for motor neuron physiology, including those that regulate the normal distributions of SMN-associated GEMs in the nucleus and mitochondria in the cytoplasm...
  47. ncbi Insulin resistance and a diabetes mellitus-like syndrome in mice lacking the protein kinase Akt2 (PKB beta)
    H Cho
    Department of Biology, University of Pennsylvania, Philadelphia, PA 19104, USA
    Science 292:1728-31. 2001
    ..These data establish Akt2 as an essential gene in the maintenance of normal glucose homeostasis...
  48. ncbi Localized Igf-1 transgene expression sustains hypertrophy and regeneration in senescent skeletal muscle
    A Musaro
    Cardiovascular Research Center, Massachusetts General Hospital East, Charlestown, Massachusetts, USA
    Nat Genet 27:195-200. 2001
    ..The preservation of muscle architecture and age-independent regenerative capacity through localized mIgf-1 transgene expression suggests clinical strategies for the treatment of age or disease-related muscle frailty...
  49. ncbi Age-dependent changes in brain, CSF, and plasma amyloid (beta) protein in the Tg2576 transgenic mouse model of Alzheimer's disease
    T Kawarabayashi
    Mayo Clinic Jacksonville, Jacksonville, Florida 32224, USA
    J Neurosci 21:372-81. 2001
    ..If a similar decline occurs in human plasma, it is possible that measurement of plasma Abeta may be useful as a premorbid biomarker for AD...
  50. pmc Colony-stimulating factor 1 promotes progression of mammary tumors to malignancy
    E Y Lin
    Department of Developmental and Molecular Biology, Center for the Study of Reproductive Biology and Women s Health, Albert Einstein College of Medicine, 1300 Morris Park Ave, Bronx, NY 10461, USA
    J Exp Med 193:727-40. 2001
    ..Our data suggest that agents directed at CSF-1/CSF-1R activity could have important therapeutic effects...
  51. pmc Progression to malignancy in the polyoma middle T oncoprotein mouse breast cancer model provides a reliable model for human diseases
    Elaine Y Lin
    Center for Study of Reproductive Biology and Women s Health, Departments of Developmental and Molecular Biology Obstetrics and Gynecology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    Am J Pathol 163:2113-26. 2003
    ..An increased leukocytic infiltration was also closely associated with the malignant transition. This study demonstrates that the PyMT mouse model is an excellent one to understand the biology of tumor progression in humans...
  52. pmc Wild-type human TDP-43 expression causes TDP-43 phosphorylation, mitochondrial aggregation, motor deficits, and early mortality in transgenic mice
    Ya Fei Xu
    Department of Neuroscience, Mayo Clinic, Jacksonville, Florida 32224, USA
    J Neurosci 30:10851-9. 2010
    ..This TDP-43 transgenic line provides a valuable tool for identifying potential roles of wild-type TDP-43 within the CNS and for studying TDP-43-associated neurotoxicity...
  53. ncbi CX3CR1-mediated dendritic cell access to the intestinal lumen and bacterial clearance
    Jan Hendrik Niess
    Gastrointestinal Unit, Massachusetts General Hospital and Harvard Medical School, 55 Fruit Street, Boston, MA 02114, USA
    Science 307:254-8. 2005
    ..Thus, CX3CR1-dependent processes, which control host interactions of specialized DCs with commensal and pathogenic bacteria, may regulate immunological tolerance and inflammation...
  54. pmc Mutant LRRK2(R1441G) BAC transgenic mice recapitulate cardinal features of Parkinson's disease
    Yanping Li
    Department of Neurology and Neurosciences, Weill Medical College of Cornell University, New York, New York, USA
    Nat Neurosci 12:826-8. 2009
    ..These mice provide a valid model of Parkinson's disease and are a resource for the investigation of pathogenesis and therapeutics...
  55. pmc Regulation of myostatin activity and muscle growth
    S J Lee
    Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 98:9306-11. 2001
    ..To determine the functional significance of these interactions in vivo, we generated transgenic mice expressing high levels of the propeptide, follistatin, or a dominant-negative form of Act RIIB by using a ..
  56. ncbi A calcineurin-dependent transcriptional pathway for cardiac hypertrophy
    J D Molkentin
    Department of Molecular Biology and Oncology, The University of Texas Southwestern Medical Center at Dallas, 75225 9148, USA
    Cell 93:215-28. 1998
    ..b>Transgenic mice that express activated forms of calcineurin or NF-AT3 in the heart develop cardiac hypertrophy and heart ..
  57. ncbi Differential antigen processing by dendritic cell subsets in vivo
    Diana Dudziak
    Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10021, USA
    Science 315:107-11. 2007
    ..This difference in antigen processing is intrinsic to the DC subsets and is associated with increased expression of proteins involved in MHC processing...
  58. ncbi RAG-2-deficient mice lack mature lymphocytes owing to inability to initiate V(D)J rearrangement
    Y Shinkai
    Howard Hughes Medical Institute, College of Physicians and Surgeons, Columbia University, New York, New York 10032
    Cell 68:855-67. 1992
    ..Because the SCID phenotype was the only obvious abnormality detected in RAG-2 mutant mice, RAG-2 function and V(D)J recombinase activity, per se, are not required for development of cells other than lymphocytes...
  59. ncbi TGFbeta in the context of an inflammatory cytokine milieu supports de novo differentiation of IL-17-producing T cells
    Marc Veldhoen
    Division of Molecular Immunology, MRC National Institute for Medical Research, Mill Hill, London NW7 1AA, United Kingdom
    Immunity 24:179-89. 2006
    ..Our data indicate that, in the presence of IL-6, TGFbeta1 subverts Th1 and Th2 differentiation for the generation of IL-17-producing T cells...
  60. pmc Pulmonary expression of interleukin-13 causes inflammation, mucus hypersecretion, subepithelial fibrosis, physiologic abnormalities, and eotaxin production
    Z Zhu
    Section of Pulmonary and Critical Care Medicine, Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut 06520 8057, USA
    J Clin Invest 103:779-88. 1999
    ..10-kDa protein promoter was used to express IL-13 selectively in the lung, and the phenotype of the resulting transgenic mice was characterized...
  61. ncbi In vivo enhancer analysis of human conserved non-coding sequences
    Len A Pennacchio
    US Department of Energy Joint Genome Institute, Walnut Creek, California 94598, USA
    Nature 444:499-502. 2006
    ..The testing of the top predictions in transgenic mice resulted in a threefold enrichment for sequences with forebrain enhancer activity...
  62. ncbi Stem cells in the hair follicle bulge contribute to wound repair but not to homeostasis of the epidermis
    Mayumi Ito
    Department of Dermatology, Kligman Laboratories, University of Pennsylvania School of Medicine, M8 Stellar Chance Building, 422 Curie Boulevard, Philadelphia, Pennsylvania 19104, USA
    Nat Med 11:1351-4. 2005
    ..Our findings have implications for both gene therapy and developing treatments for wounds because it will be necessary to consider epidermal and hair follicle stem cells as distinct populations...
  63. pmc Expression of the neu protooncogene in the mammary epithelium of transgenic mice induces metastatic disease
    C T Guy
    Institute for Molecular Biology and Biotechnology, McMaster University, Hamilton, Ontario, Canada
    Proc Natl Acad Sci U S A 89:10578-82. 1992
    ..To directly assess the effect of mammary gland-specific expression of the neu protooncogene, transgenic mice carrying unactivated neu under the transcriptional control of the mouse mammary tumor virus promoter/enhancer ..
  64. ncbi A critical role for Dnmt1 and DNA methylation in T cell development, function, and survival
    P P Lee
    Department of Immunology, University of Washington, Seattle, WA 98195, USA
    Immunity 15:763-74. 2001
    ..We conclude that Dnmt1 and DNA methylation are required for the proper expression of certain genes that define fate and determine function in T cells...
  65. pmc Mapping Wnt/beta-catenin signaling during mouse development and in colorectal tumors
    Silvia Maretto
    Histology and Embryology Section, Department of Histology, Microbiology, and Medical Biotechnology, University of Padua, 35131 Padua, Italy
    Proc Natl Acad Sci U S A 100:3299-304. 2003
    ..the beta-catenin-activated transgene driving expression of nuclear beta-galactosidase reporter (BAT-gal) transgenic mice, expressing the lacZ gene under the control of beta-cateninT cell factor responsive elements...
  66. ncbi Formation of neurofibrillary tangles in P301l tau transgenic mice induced by Abeta 42 fibrils
    J Gotz
    Division of Psychiatry Research, University of Zurich, August Forel Strasse 1, 8008 Zurich, Switzerland
    Science 293:1491-5. 2001
    ..Injection of beta-amyloid Abeta42 fibrils into the brains of P301L mutant tau transgenic mice caused fivefold increases in the numbers of NFTs in cell bodies within the amygdala from where neurons ..
  67. ncbi Tolerance induction in double specific T-cell receptor transgenic mice varies with antigen
    H Pircher
    Department of Experimental Pathology, University Hospital, Zurich, Switzerland
    Nature 342:559-61. 1989
    ..We have now studied T-cell tolerance in transgenic mice expressing a T-cell receptor with double specificities for lymphocytic choriomeningitis virus (LCMV)-H-2Db ..
  68. ncbi Intranuclear inclusions and neuritic aggregates in transgenic mice expressing a mutant N-terminal fragment of huntingtin
    G Schilling
    Department of Psychiatry, Division of Neuropathology, Johns Hopkins University, Baltimore, MD 21205 2196, USA
    Hum Mol Genet 8:397-407. 1999
    ..To gain insight into the pathogenesis of HD, we generated transgenic mice that express a cDNA encoding an N-terminal fragment (171 amino acids) of huntingtin with 82, 44 or 18 ..
  69. ncbi Neural stem and progenitor cells in nestin-GFP transgenic mice
    John L Mignone
    Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
    J Comp Neurol 469:311-24. 2004
    ..We used the regulatory elements of the nestin gene to generate transgenic mice in which neural stem cells of the embryonic and adult brain are marked by the expression of green fluorescent ..
  70. pmc MCP-1 contributes to macrophage infiltration into adipose tissue, insulin resistance, and hepatic steatosis in obesity
    Hajime Kanda
    Department of Clinical Molecular Medicine, Kobe University Graduate School of Medicine, Kobe, Japan
    J Clin Invest 116:1494-505. 2006
    ..These findings suggest that an increase in MCP-1 expression in adipose tissue contributes to the macrophage infiltration into this tissue, insulin resistance, and hepatic steatosis associated with obesity in mice...
  71. ncbi Dopaminergic loss and inclusion body formation in alpha-synuclein mice: implications for neurodegenerative disorders
    E Masliah
    Department of Neurosciences, Department of Pathology, University of California San Diego, La Jolla, CA 92093 0624, USA
    Science 287:1265-9. 2000
    To elucidate the role of the synaptic protein alpha-synuclein in neurodegenerative disorders, transgenic mice expressing wild-type human alpha-synuclein were generated...
  72. ncbi Looking BAC at striatal signaling: cell-specific analysis in new transgenic mice
    Emmanuel Valjent
    INSERM, UMR S 839, 75005 Paris, France
    Trends Neurosci 32:538-47. 2009
    ..The recent development of bacterial artificial chromosome (BAC) transgenic mice expressing a variety of reporters, epitope tagged-proteins or Cre recombinase driven by specific promoters, ..
  73. pmc Use of the viral 2A peptide for bicistronic expression in transgenic mice
    Georgios Trichas
    Department of Physiology Anatomy and Genetics, University of Oxford, Oxford, UK
    BMC Biol 6:40. 2008
    ..They work in a wide variety of eukaryotic cells, but to date have not been demonstrated to function in transgenic mice in an inheritable manner.
  74. pmc Electroporation and RNA interference in the rodent retina in vivo and in vitro
    Takahiko Matsuda
    Department of Genetics and Howard Hughes Medical Institute, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 101:16-22. 2004
    ..Plasmid DNA was also efficiently transfected into retinal explants in vitro by high-voltage pulses...
  75. pmc Remote control of neuronal activity in transgenic mice expressing evolved G protein-coupled receptors
    Georgia M Alexander
    Department of Neurobiology, Duke University, Durham, NC 27710, USA
    Neuron 63:27-39. 2009
    ..To overcome these barriers, we created transgenic mice expressing an evolved G protein-coupled receptor (hM3Dq) selectively activated by the pharmacologically inert,..
  76. ncbi Macrophages regulate the angiogenic switch in a mouse model of breast cancer
    Elaine Y Lin
    Department of Developmental and Molecular Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Cancer Res 66:11238-46. 2006
    ..Taken together, this study shows that tumor-associated macrophages play a key role in promoting tumor angiogenesis, an essential step in the tumor progression to malignancy...
  77. ncbi Amyotrophic lateral sclerosis is a distal axonopathy: evidence in mice and man
    Lindsey R Fischer
    Department of Neurology, Emory University School of Medicine, Atlanta, GA 30322, USA
    Exp Neurol 185:232-40. 2004
    ..We conclude that in this widely studied animal model of human ALS, and in this single human case, motor neuron pathology begins at the distal axon and proceeds in a "dying back" pattern...
  78. ncbi Initial sequencing and comparative analysis of the mouse genome
    Robert H Waterston
    Genome Sequencing Center, Washington University School of Medicine, Campus Box 8501, 4444 Forest Park Avenue, St Louis, Missouri 63108, USA
    Nature 420:520-62. 2002
    ....
  79. ncbi Fate of the mammalian cardiac neural crest
    X Jiang
    Departments of Biological Sciences and Cell and Neurobiology, Institute for Genetic Medicine, Keck School of Medicine, University of Southern California, IGM 240, Los Angeles, CA 90033, USA
    Development 127:1607-16. 2000
    ....
  80. ncbi Formation of neuronal intranuclear inclusions underlies the neurological dysfunction in mice transgenic for the HD mutation
    S W Davies
    Department of Anatomy and Developmental Biology, University College London, United Kingdom
    Cell 90:537-48. 1997
    ..The appearance in transgenic mice of these inclusions, followed by characteristic morphological change within neuronal nuclei, is strikingly ..
  81. ncbi Wild-type nonneuronal cells extend survival of SOD1 mutant motor neurons in ALS mice
    A M Clement
    Ludwig Institute for Cancer Research, University of California, 9500 Gilman Drive, La Jolla, CA 92093 0670, USA
    Science 302:113-7. 2003
    ..Normal motor neurons in SOD1 mutant chimeras develop aspects of ALS pathology. Most important, nonneuronal cells that do not express mutant SOD1 delay degeneration and significantly extend survival of mutant-expressing motor neurons...
  82. ncbi Efficient method to generate single-copy transgenic mice by site-specific integration in embryonic stem cells
    Caroline Beard
    Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, USA
    Genesis 44:23-8. 2006
    ..This strategy and the vectors described here are generally applicable to any locus in ES cells and should allow for the rapid production of mice with transgenes efficiently targeted to a defined site...
  83. ncbi A muscle-specific insulin receptor knockout exhibits features of the metabolic syndrome of NIDDM without altering glucose tolerance
    J C Bruning
    Joslin Diabetes Center, Department of Medicine, Harvard Medical School, Boston, Massachusetts 02215, USA
    Mol Cell 2:559-69. 1998
    ..Thus, insulin resistance in muscle contributes to the altered fat metabolism associated with type 2 diabetes, but tissues other than muscle appear to be more involved in insulin-regulated glucose disposal than previously recognized...
  84. ncbi Development of TH1 CD4+ T cells through IL-12 produced by Listeria-induced macrophages
    C S Hsieh
    Department of Pathology, Washington University School of Medicine, St Louis, MO 63110
    Science 260:547-9. 1993
    ..Therefore, this regulatory pathway may have evolved to enable innate immune cells, through interactions with microbial pathogens, to direct development of specific immunity toward the appropriate TH phenotype...
  85. pmc p63 is the molecular switch for initiation of an epithelial stratification program
    Maranke I Koster
    Program in Developmental Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Genes Dev 18:126-31. 2004
    ..Our data demonstrate that p63 plays a dual role: initiating epithelial stratification during development and maintaining proliferative potential of basal keratinocytes in mature epidermis...
  86. ncbi Osteoprotegerin: a novel secreted protein involved in the regulation of bone density
    W S Simonet
    Department of Molecular Genetics, Amgen Inc, Thousand Oaks, California 91320, USA
    Cell 89:309-19. 1997
    ..In vivo, hepatic expression of OPG in transgenic mice results in a profound yet nonlethal osteopetrosis, coincident with a decrease in later stages of osteoclast ..
  87. pmc Selective depletion of Foxp3+ regulatory T cells induces a scurfy-like disease
    Katharina Lahl
    Institut fur Medizinische Mikrobiologie, Immunologie und Hygiene, Technische Universitat Munchen, 81675 Munich, Germany
    J Exp Med 204:57-63. 2007
    ..We describe the generation of bacterial artificial chromosome-transgenic mice termed "depletion of regulatory T cell" (DEREG) mice expressing a diphtheria toxin (DT) receptor-enhanced ..
  88. ncbi Extension of murine life span by overexpression of catalase targeted to mitochondria
    Samuel E Schriner
    Department of Genome Sciences, University of Washington, Seattle, WA 91895, USA
    Science 308:1909-11. 2005
    To determine the role of reactive oxygen species in mammalian longevity, we generated transgenic mice that overexpress human catalase localized to the peroxisome, the nucleus, or mitochondria (MCAT)...
  89. ncbi Aberrant excitatory neuronal activity and compensatory remodeling of inhibitory hippocampal circuits in mouse models of Alzheimer's disease
    Jorge J Palop
    Gladstone Institute of Neurological Disease, San Francisco, CA 94158, USA
    Neuron 55:697-711. 2007
    ..Neuronal circuits vulnerable to AD are also affected in human amyloid precursor protein (hAPP) transgenic mice. hAPP mice with high levels of amyloid-beta peptides in the brain develop AD-like abnormalities, including ..
  90. ncbi Interleukin-7 mediates the homeostasis of naïve and memory CD8 T cells in vivo
    K S Schluns
    Department of Medicine, University of Connecticut Health Center, Farmington, CT 06030, USA
    Nat Immunol 1:426-32. 2000
    ..Homeostatic proliferation of memory cells was also partially dependent on IL-7. These results point to IL-7 as a pivotal cytokine in T cell homeostasis...
  91. pmc Skeletal muscle-restricted expression of human SOD1 causes motor neuron degeneration in transgenic mice
    Margaret Wong
    Division of Neuropathology, Department of Pathology, Johns Hopkins University School of Medicine, 558 Ross Building, 720 Rutland Avenue, Baltimore, MD 21205 2196, USA
    Hum Mol Genet 19:2284-302. 2010
    ..The discovery of instigating molecular toxicities or disease progression determinants within skeletal muscle could be very valuable for the development of new effective therapies for the treatment and cure of ALS...
  92. ncbi Bone morphogenetic proteins
    Di Chen
    School of Medicine and Dentistry, Department of Orthopaedics, University of Rochester, Rochester, NY 14642, USA
    Growth Factors 22:233-41. 2004
    ..A significant advancement about the understanding of in vivo functions of BMP ligands, receptors and signaling molecules has been achieved in recent years...
  93. pmc In vivo depletion of CD11c+ dendritic cells abrogates priming of CD8+ T cells by exogenous cell-associated antigens
    Steffen Jung
    Molecular Pathogenesis Program, Skirball Institute of Biomolecular Medicine, New York University School of Medicine, New York 10016, USA
    Immunity 17:211-20. 2002
    ..e., the sensitization of the immune system for cell-associated antigens. DC-depleted mice fail to mount CTL responses to infection with the intracellular bacterium Listeria monocytogenes and the rodent malaria parasite Plasmodium yoelii...
  94. ncbi Effector and memory CD8+ T cell fate coupled by T-bet and eomesodermin
    Andrew M Intlekofer
    Abramson Family Cancer Research Institute and Department of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Nat Immunol 6:1236-44. 2005
    ..Therefore, these key transcription factors link the long-term renewal of memory CD8(+) T cells to their characteristic effector potency...
  95. ncbi A beta peptide vaccination prevents memory loss in an animal model of Alzheimer's disease
    D Morgan
    Department of Pharmacology, University of South Florida, Tampa 33612, USA
    Nature 408:982-5. 2000
    ..Here we show that vaccination with A beta protects transgenic mice from the learning and age-related memory deficits that normally occur in this mouse model for Alzheimer's ..
  96. ncbi Early-onset amyloid deposition and cognitive deficits in transgenic mice expressing a double mutant form of amyloid precursor protein 695
    M A Chishti
    Centre for Research in Neurodegenerative Diseases, The Department of Laboratory Medicine, Division of Neurology, University Health Network, University of Toronto, Toronto, Ontario M5S 3H2, Canada
    J Biol Chem 276:21562-70. 2001
    ..The Tg mice described here suggest a potential to investigate aspects of Alzheimer's disease pathogenesis, prophylaxis, and therapy within short time frames...
  97. pmc Loss of Apc in vivo immediately perturbs Wnt signaling, differentiation, and migration
    Owen J Sansom
    School of Biosciences, University of Cardiff, Cardiff CF10 3US, Wales
    Genes Dev 18:1385-90. 2004
    ..Critically, for the first time we confirm a series of Wnt target molecules in an in vivo setting and also identify a series of new candidate targets within the same setting...
  98. ncbi Regulation of cerebral cortical size by control of cell cycle exit in neural precursors
    Anjen Chenn
    Department of Pathology, Brigham and Women s Hospital, Boston, MA 02115, USA
    Science 297:365-9. 2002
    b>Transgenic mice expressing a stabilized beta-catenin in neural precursors develop enlarged brains with increased cerebral cortical surface area and folds resembling sulci and gyri of higher mammals...
  99. ncbi Severe hypercholesterolemia and atherosclerosis in apolipoprotein E-deficient mice created by homologous recombination in ES cells
    A S Plump
    Laboratory of Biochemical Genetics and Metabolism, The Rockefeller University, New York, New York 10021 6399
    Cell 71:343-53. 1992
    ..apoE-deficient mice are a promising small animal model to help understand the role of apoE in vivo and the genetic and environmental determinants of atherosclerosis...
  100. pmc Astrocytes expressing ALS-linked mutated SOD1 release factors selectively toxic to motor neurons
    Makiko Nagai
    Department of Neurology, Columbia University, 710 West 168th Street, New York, New York 10032, USA
    Nat Neurosci 10:615-22. 2007
    ..Identification of the astrocyte-derived soluble factor(s) may have far-reaching implications for ALS from both a pathogenic and therapeutic standpoint...
  101. ncbi Inhibition of mTOR induces autophagy and reduces toxicity of polyglutamine expansions in fly and mouse models of Huntington disease
    Brinda Ravikumar
    Department of Medical Genetics, Cambridge Institute for Medical Research, Wellcome MRC Building, Addenbrooke s Hospital, Hills Road, Cambridge CB2 2XY, UK
    Nat Genet 36:585-95. 2004
    ..we show that mammalian target of rapamycin (mTOR) is sequestered in polyglutamine aggregates in cell models, transgenic mice and human brains...

Research Grants72

  1. NIH Director's Pioneer Award
    Junying Yuan; Fiscal Year: 2009
    ..No Abstract provided ..
  2. Transcription factors regulating sensory gene expression and pain pathways
    Eric E Turner; Fiscal Year: 2011
    ..To determine the complete set of pain pathway genes dependent on Islet1, we will use transgenic mice expressing an inducible Cre-recombinase from the Islet1 gene locus to delete Islet1 after sensory ..
  3. Mice Humanized with Hepatocytes and iPS Cells from Patients with Metabolic Diseas
    Stephen C Strom; Fiscal Year: 2010
    ..We propose these humanized mice will be a technology platform to better understand the human disease and it will aid in the development of gene or cellular therapy to correct these devastating diseases. ..
  4. Role of the Y-Located TSPY Gene in Human Oncogenesis
    Yun Fai Chris Lau; Fiscal Year: 2013
    ..TSPY induces gonadoblastoma-like structure in the ovaries of transgenic mice. TSPX is a tumor suppressor. It binds cyclin B, but represses the cyclin B-CDK1 kinase activity...
  5. Identification of Genetic and Epigenetic Alterations in Spondyloarthritis
    Tibor T Glant; Fiscal Year: 2013
    ..In Aim 1B, we will generate transgenic mice carrying (non-mutated) Hmcn2 alone or in combination with Gpr107 and Nsc1 of non-DBA/2 origin (B6) to reverse ..
  6. Pilot and definitive studies to evaluate Insertional Mutagenesis
    LU TAYLOR; Fiscal Year: 2012
    ..Perinatal study of polybrominated diphenyl ether congener DE-47 in CB6F1-Tg(HRAS2) transgenic mice: A pilot study is being performed at the lab and the results are expected in FY 2007...
  7. Studies to Evaluate the Toxicological Potential of Test Articles
    LU TAYLOR; Fiscal Year: 2010
    ..Perinatal study of polybrominated diphenyl ether congener DE-47 in CB6F1-Tg(HRAS2) transgenic mice: A pilot study is being performed at the lab and the results are expected in FY 2007...
  8. Physiological role of PRDM16 in brown fat development and energy balance
    Patrick Seale; Fiscal Year: 2011
    ..The transgenic mice were generated in the FVB background and this inbred strain is not prone to high fat diet-induced obesity...
  9. Targeting Galectin-3 with novel drugs for treatment of eczema
    Fu Tong Liu; Fiscal Year: 2010
    ..Through extensive studies of transgenic mice and human tissues, we have identified galectin-3 as a key molecule involved in skin inflammation...
  10. Gamma-Synuclein-Mediated Regulation of Norepinephrine Transporter
    Anita Sidhu; Fiscal Year: 2010
    ..Syn overexpressing transgenic mice, as well as in .-Syn knock-out mice...
  11. Regulation of thyroid hormone levels by central and peripheral pathways
    KRISTEN RACHEL VELLA; Fiscal Year: 2013
    ..These mutant receptors are targeted to specific subsets of neurons using the Cre-LoxP recombination system in transgenic mice. By administering CNO to mice expressing DREADDs in NPY or MC4R neurons, this technology will pinpoint the ..
  12. ROLE OF Rac AND REACTIVE OXYGEN SPECIES IN KAPOSI'S SARCOMA VIRAL ONCOGENESIS
    Enrique A Mesri; Fiscal Year: 2013
    ..expression of a constitutively-active Rac1 mutant (RacCA) driven by -smooth muscle actin (-SMA) promoter in transgenic mice led to the formation of lesions that strongly resemble those of Kaposi's sarcoma...
  13. The role of Fli1 in T cell function and the pathogenesis of lupus
    Tamara K Nowling; Fiscal Year: 2011
    ..has effects on the immune system and the pathogenesis of lupus as evidenced by the immune phenotype of Fli1 transgenic mice and the profound effect of Fli1 heterozygosity on a mouse model of lupus...
  14. Epigenetic mechanisms relevant to the pathogenesis of ALS
    Neil W Kowall; Fiscal Year: 2013
    ..The urgent need for such studies is highlighted by the prospect that hundreds of thousands of future veterans may be at increased risk for this devastating disease. ..
  15. Effect of PPARy Ligands on Alcohol-Induced Alveolar Macrophage Oxidative Stress
    Samantha M Yeligar; Fiscal Year: 2013
    ..models using intranasal administration, and c) skills required to develop and manage colonies of knockout and transgenic mice. These skills will be acquired through the execution of the proposed studies with the assistance and training ..
  16. Mechanisms of action of ghrelin in muscle and adipose tissue in cancer-related ca
    Jose M Garcia; Fiscal Year: 2013
    ..Cachexia is also a complication of many other conditions including lung and heart disease and aging, the knowledge generated through this proposal also will help us in establishing new therapies for these conditions. ..
  17. Med1 in Liver Metabolism, Regeneration and Cancer
    Janardan K Reddy; Fiscal Year: 2013
    ....
  18. IMMUNOGENETICS OF COLLAGEN INDUCED ARTHRITIS IN MICE
    Chella David; Fiscal Year: 2009
    ..Collagen-induced arthritis (CIA) susceptible HLA transgenic mice produce rheumatoid factor and anti-cyclic citrullinated peptide (CCP) antibodies similar to that in RA ..
  19. Statin modulation of immunotherapy for Alzheimer disease
    Jun Tan; Fiscal Year: 2012
    ..A different anti-AD strategy, vaccination with the A( peptide, also reduces A( deposition in AD transgenic mice and improves cognition...
  20. MOLECULAR BASIS OF REOVIRUS PATHOGENESIS
    Terence S Dermody; Fiscal Year: 2013
    ..b>Transgenic mice in which JAM-A is either expressed or ablated solely in endothelial or hematopoietic cells will be infected ..
  21. DNA methylation of extracellular superoxide dismutase in pulmonary hypertension
    EVA NOZIK GRAYCK; Fiscal Year: 2012
    ..Augmenting EC- SOD activity in transgenic mice or through adenoviral gene delivery attenuates pulmonary vascular remodeling and pulmonary hypertension...
  22. Mitochondrial Dysfunction and Adipose Insulin Resistance
    David A Bernlohr; Fiscal Year: 2013
    ..Specific Aim 3. Develop and characterize aP2-HA-GSTA4 transgenic mice maintained on low and high fat diets. Specific Aim 4...
  23. Remodeling Neuronal Chromatin in Mouse Models for Depression
    Schahram Akbarian; Fiscal Year: 2013
    ..focus of this preclinical proposal is to explore the link between antidepressant-like phenotypes of Setdb1 transgenic mice, and the transcriptional regulation of NMDA receptor subunit Grin2b, and other gene expression changes, and ..
  24. Monitoring cellular redox signaling and oxidant stress in vivo
    Paul T Schumacker; Fiscal Year: 2010
    ..In Aim 1 we will create transgenic mice with DNA encoding the redox-sensitive fluorescent protein, RoGFP, inserted at a LoxP-silenced ROSA26 genomic ..
  25. Prevention of UV-induced carcinogenesis by cyanidin-3-glucoside
    Xianglin Shi; Fiscal Year: 2013
    ..of activation protein (AP)-1, nuclear factor (NF)-:B, and nuclear factor of activated T cells (NAFT) in transgenic mice. Specific Aim 3. Investigate the effects of C3G on UVB-induced inflammatory mediators...
  26. Central Action of Brain-Derived Neurotrophic Factor in Male and Female Rats
    Haifei Shi; Fiscal Year: 2010
    ..The gender difference in BDNF signaling in energy balance has been suggested from studies using transgenic mice with dysfunctional BDNF system...
  27. Arcuate nucleus glutamatergic neurons modulate energy homeostasis
    Anthony N van den Pol; Fiscal Year: 2013
    ..abstract_text> ..
  28. Regulation of Bile Acid Synthesis by Nuclear Receptors
    John Y L Chiang; Fiscal Year: 2013
    ..We have shown that transgenic expression of Cyp7a1 in mice (Cyp7a1-transgenic mice) protected mice from diet-induced hepatic steatosis, insulin resistance and obesity...
  29. Sirtuin/FOXO Signaling in the Regulation of Bone Mass
    Stavroula Kousteni; Fiscal Year: 2012
    ..Conversely, transgenic mice expressing SIRT1 show enhanced deacetylation of FOXO1 in bone, increased bone mass in the spine and femur and ..
  30. Regulation of Type 1 Interferon Production by Autoimmunity-Associated PTPN22
    Yaya Wang; Fiscal Year: 2013
    ..In Aim 2, I will address the impact of LypW on TLR signaling by using BMMs and BMDCs from LypW transgenic mice. I will also investigate the role of LypW in pathogenesis of rheumatoid arthritis using the K/BxN TCR ..
  31. Mechanisms of Endothelin 1-Induced Sickle Cell Pathology
    Alicia Rivera; Fiscal Year: 2011
    ..Our preliminary data indicate that sickle transgenic mice treated with ET-1 receptor antagonists have reduce SRBC cellular dehydration...
  32. Alzheimer's Disease Therapeutic
    Trevor P Castor; Fiscal Year: 2012
    ..Bryostatin 1 was effective in reducing brain amyloid plaques (A240 and A242 in AD) in double-transgenic mice while improving behavioral outcomes and the rate of premature death...
  33. ER stress: role in myocyte apoptosis and myocardial remodeling
    Krishna Singh; Fiscal Year: 2013
    ..Specific aim 4 will use transgenic mice with cardiac-specific overexpression of dominant negative GSK-32 to investigate the role of GSK-32 in cardiac ..
  34. Epigenomic Events in Development of the Locus Coeruleus Noradrenergic Neurons
    Jan Krzysztof Blusztajn; Fiscal Year: 2012
    ..to purify defined neuronal populations throughout lifespan by fluorescence-activated cell sorting using transgenic mice that express enhanced green fluorescent protein (EGFP) under the control of the regulatory elements of genes ..
  35. Role of Mitochondria in the Regulation of Iron metabolism by Alcohol
    DUYGU DEE DEE HARRISON-FINDIK; Fiscal Year: 2012
    ..The knowledge obtained from these studies, characterizing the pivotal role of mitochondria, will enable us to determine the mechanisms of liver injury caused by iron and alcohol in patients with ALD. ..
  36. Conditional HSF1 Expression for Ischemic Cardioprotection
    Ivor J Benjamin; Fiscal Year: 2012
    ..If successful, such beneficial outcomes may improve the health of veterans with acute ischemic syndromes during cardiac transplantation, coronary bypass surgery, and other acquired cardiac conditions. ..
  37. Bioassay for Breast Cancer Prevention
    JoEllen Welsh; Fiscal Year: 2010
    ..Alternatively, of course, one could use knockout or transgenic mice as donors to probe specific gene-nutrient interactions in modulation of DNA damage response pathways in the ..
  38. Air Pollution on Adiposity and Vascular Dysfunction: White or Brown Matters?
    Qinghua Sun; Fiscal Year: 2010
    ..5 exposure on white and brown adipocytes and associated vascular dysfunction in transgenic mice expressing yellow fluorescent protein (YFP) under the control of monocyte-macrophage specific promoter (c-fms,..
  39. Cellular and Animal Models of FUS Mutations in ALS
    Eric J Huang; Fiscal Year: 2013
    ..investigate the mechanisms of mutant FUS proteins in neuronal degeneration using both primary neurons and transgenic mice. Since synaptic degeneration plays a central role in neurodegenerative diseases, we believe that the ..
  40. Characterization of epsilon-sarcoglycan interacting proteins in mouse brain
    Yuqing Li; Fiscal Year: 2012
    ..The broad, long-term objective of our research is to use transgenic mice to determine: 1) the functional role of ?-SG in vivo 2) how the loss of 5-SG protein leads to M-D...
  41. Preclinical drug trial in mouse models of inflammation
    Kanneboyina Nagaraju; Fiscal Year: 2011
    ..This proposal will help to train a pool of talented young scientists to pursue mouse model research and accelerate development of therapies for inflammatory diseases. ..
  42. Role of GDNF in the regulation of hepatic steatosis
    SHANTHI K SRINIVASAN; Fiscal Year: 2013
    ..Recently we discovered that (i) GDNF transgenic mice when fed a high fat diet resist weight gain and development of hepatic steatosis, despite similar food intake ..
  43. ROLE OF SIRTUIN IN CIGARETTE SMOKE-INDUCED LUNG INFLAMMATION AND INJURY
    Irfan Rahman; Fiscal Year: 2013
    ..associated with increased influx of neutrophils, whereas these responses were attenuated in lungs of SIRT1 transgenic mice exposed to CS...
  44. Runx Proteins and Sex Steroid Signaling in Bone
    Baruch Frenkel; Fiscal Year: 2009
    ..These inhibitory activities are important in light of recent data from [unreadable]1(I)collagen-Runx2 transgenic mice, indicating that Runx2 must be restrained in order to keep bone turnover in check and prevent osteoporosis...
  45. Mouse models of genomic instability and birth defects
    CATHERINE ELIZABETH KEEGAN; Fiscal Year: 2013
    ....
  46. Alcohol-Induced Bone Resorption: The Role of Oxidative Stress
    Martin J J Ronis; Fiscal Year: 2013
    ..role of NOX4 and oxidative stress in EtOH-induced bone resorption in vivo using p47phox -/- mice and catalase transgenic mice. In vitro, the mouse ST-2 cell line, differentiated mouse stromal osteoblast cultures and osteoblast/..
  47. Mitochondrial Dysfunction and Adipose Insulin Resistance
    DAVID BERNLOHR; Fiscal Year: 2009
    ..Aim 3. Develop and characterize aP2-HA-GSTA4 transgenic mice maintained on low and high fat diets. Aim 4...
  48. The Role of Astrocytes in Cortical Interneuron Development
    KAREN MULLER SMITH; Fiscal Year: 2013
    ..through mentored research training in primary tissue culture, cell transplantation, behavioral analysis of transgenic mice, gene expression microarray experiments using the translating ribosome affinity purification (TRAP) method, ..
  49. Regulation of vaccine-induced anti-fungal T17 cells
    MARCEL WUETHRICH; Fiscal Year: 2013
    ..In this proposal, we will identify the host-pathogen interactions that are the foundation of vaccine-induced resistance. The knowledge gained from this research will lead to the development of fungal vaccines and adjuvants. ..
  50. Epithelial differentiation in cornea
    Bogi Andersen; Fiscal Year: 2012
    ..To investigate the role of Clims, we generated transgenic mice expressing a dominant-negative Clim molecule (DN-Clim) under the control of the keratin 14 promoter (K14-DN-..
  51. Molecular Mechanism of Immune Therapy for Bone Marrow Failures
    Pearlie K Epling-Burnette; Fiscal Year: 2013
    ..Additionally, the efficacy of pharmacological inhibitors to block homing to the bone marrow will be tested to provide critical pre-clinical data for application to clinical studies in patients in the future. ..
  52. Combined BAC Transgenic and Knock-Out Mouse Model of Lowe Syndrome Nephropathy
    Robert L Nussbaum; Fiscal Year: 2013
    ..We created transgenic mice deleted for both murine Ocrl and Inpp5b and hemizygous for a bacterial artificial chromosome (BAC) containing ..
  53. P38 MAP KINASE SIGNALING IN SKIN
    Tatiana Efimova; Fiscal Year: 2013
    ..silencing of p384 gene in a temporally regulated fashion, by means of crossing p384flox mice to transgenic mice in which a tamoxifen-regulated CreERT recombinase is expressed under the control of keratinocyte-specific ..
  54. MECHANISMS OF MILK LIPID SECRETION
    JAMES LEWIS MCMANAMAN; Fiscal Year: 2013
    ..b>Transgenic mice expressing N- and C-terminally truncated forms of ADPH will be used to define effects of specific ADPH ..
  55. Mechanistic Studies on Prostate Cancer Prevention by Gugulipid
    Dong Xiao; Fiscal Year: 2013
    ..prostate cancer by GL and to determine efficacy of GL for prevention of prostate carcinogenesis using TRAMP transgenic mice. GL is a promising compound and has significant potential in preventive applications since it has selectively ..
  56. Roles of NF-kB/Rel in the pathogenesis of breast cancer
    Gail E Sonenshein; Fiscal Year: 2012
    ..Thus, pre-clinical testing of green tea components on breast cancer cells that are resistant to antibody therapy will be performed, and if successful, this approach can readily be translated to the clinic. ..
  57. Role of Platelet derived growth factor receptor-a in Liver Patho-biology
    SATDARSHAN SINGH MONGA; Fiscal Year: 2013
    ..basis of the disease process in absence or overexpression of PDGFR[unreadable] in hepatocytes in novel transgenic mice in our lab and complemented by utilization of species-specific PDGFR[unreadable] blocking antibodies to ..
  58. Ca Signaling in Progression of Amyotrophic Lateral Sclerosis in Skeletal Muscle
    Jingsong Zhou; Fiscal Year: 2013
    ....
  59. Osteoclastic Protein-Tyrosine Phosphatase and Resorption
    Kin Hing William Lau; Fiscal Year: 2013
    ..cells led to a large increase in bone resorption and a marked decrease in bone mass in male but not female transgenic mice, compared to corresponding wild-type littermates...
  60. Neuroprotection by IFN-beta in AIDS
    Marcus Kaul; Fiscal Year: 2013
    ..neuronal injury, neurogenesis and gliosis will be compared in IFN?- versus vehicle-treated HIV/gp120-transgenic mice. All three Specific Aims will test the premise that IFN? induces neuroprotective ?-chemokines and NGF, ..
  61. Contribution of c-Jun N-terminal kinase activity to vestibular schwannoma growth
    Marlan R Hansen; Fiscal Year: 2013
    ..Parallel studies will ask if SCs derived from transgenic mice lacking functional merlin demonstrate increased JNK activity...
  62. Transcriptional control of pituitary gonadotropin genes
    Varykina G Thackray; Fiscal Year: 2013
    ..Funding of this proposal will also allow the PI, as an Early-Stage Investigator, to establish a fully-independent research program in the field of reproductive endocrinology. ..
  63. MOLECULAR MECHANISMS OF MHC LINKED SUSCEPTIBILITY
    Kai Wucherpfennig; Fiscal Year: 2001
    ..b>Transgenic mice that express the PV-associated DQalpha and DQbeta genes (DQA1*0101, DQB1*0503) will be generated as an animal ..
  64. SIRT1 Overexpression in Cellular Mitochondrial Metabolism and Function
    James Russell; Fiscal Year: 2009
    ..To study this, generation of transgenic mice that conditionally expresses SIRT1 is needed...
  65. Activators of the Pyrophosphatase Activity of Alkaline Phosphatase
    Jose Luis Millan; Fiscal Year: 2007
    ..We recently tested this axis by surmising that transgenic mice over-expressing TNAP might be able to achieve tissular expression of TNAP sufficiently high to be able to ..
  66. Humanized Transgenic Mice Reproduce the Disease Evolution in Severe RA
    Grete Sonderstrup; Fiscal Year: 2010
    DESCRIPTION (provided by applicant): Humanized transgenic mice reproduce the disease evolution in severe RA Rheumatoid arthritis (RA) is a common autoimmune disease in humans genetically associated with specific HLA class II alleles...
  67. Development & Characterization of Tyrosinase Epitope Specific TCR Transgenic Mice
    Shikhar Mehrotra; Fiscal Year: 2010
    ..Since T cell receptor (TCR) transgenic mice have contributed to many aspects in understanding T cell ontogeny and selection of the peripheral repertoire, ..
  68. TRANSGENIC MICE, AGING, CANCER, AND OXIDATIVE DAMAGE
    Arlan Richardson; Fiscal Year: 2001
    ..In this proposal, transgenic mice that show reduced expression of either Mn-SOD (Sod2+/-) or both Mn-SOD and the selenium-dependent glutathione ..
  69. MECHANISMS OF AUTOIMMUNE ARTHRITIS
    EDWARD ROSLONIEC; Fiscal Year: 2001
    ..In previously published studies and preliminary data, the investigators describe the production of transgenic mice expressing chimeric MHC class II molecules in which the peptide binding domain of the beta subunit is derived ..
  70. SALIVARY GLAND TRANSGENIC MOUSE MODEL
    ELISA DURBAN; Fiscal Year: 1990
    b>Transgenic mice are powerful tools to study normal and abnormal cellular processes. A transgenic mouse strain with a high incidence of salivary gland tumors was generated by Drs...
  71. Mouse Models of ErbB-2 and Cyclin D1 in Prostate Cancer
    Christopher Albanese; Fiscal Year: 2002
    ..We propose to test this hypothesis directly using transgenic mice. Determining the role of ErbB-2 and cyclin D1 in prostate cancer progression and determining the mechanisms ..
  72. Proteoglycans/Glycosaminoglycans in APP Transgenic Mice
    ALAN SNOW; Fiscal Year: 2004
    ..and 2) Detect and Quantify Heparan Sulfate GAGs Derived from Biological Fluids of Human AD Patients and APP Transgenic Mice, and Determine Their Utility as a Diagnostic Marker for AD...