transgenic mice

Summary

Summary: Laboratory mice that have been produced from a genetically manipulated EGG or EMBRYO.

Top Publications

  1. pmc Cre reporter strains produced by targeted insertion of EYFP and ECFP into the ROSA26 locus
    S Srinivas
    Department of Genetics and Development, Columbia University, New York, USA
    BMC Dev Biol 1:4. 2001
  2. ncbi Alzheimer's disease is a synaptic failure
    Dennis J Selkoe
    Center for Neurologic Diseases, Brigham and Women s Hospital, and the Harvard Center for Neurodegeneration and Repair, Boston, MA 02115, USA
    Science 298:789-91. 2002
  3. ncbi Exon 1 of the HD gene with an expanded CAG repeat is sufficient to cause a progressive neurological phenotype in transgenic mice
    L Mangiarini
    Division of Medical and Molecular Genetics, UMDS, Guy s Hospital, London, United Kingdom
    Cell 87:493-506. 1996
  4. ncbi Motor neuron degeneration in mice that express a human Cu,Zn superoxide dismutase mutation
    M E Gurney
    Department of Cell and Molecular Biology, Northwestern University Medical School, Chicago, IL 60611
    Science 264:1772-5. 1994
  5. pmc In vivo analysis of autophagy in response to nutrient starvation using transgenic mice expressing a fluorescent autophagosome marker
    Noboru Mizushima
    Department of Cell Biology, National Institute for Basic Biology, Okazaki 444 8585, Japan
    Mol Biol Cell 15:1101-11. 2004
  6. ncbi Triple-transgenic model of Alzheimer's disease with plaques and tangles: intracellular Abeta and synaptic dysfunction
    Salvatore Oddo
    Department of Neurobiology and Behavior, University of California, Irvine, Irvine, California 92697, USA
    Neuron 39:409-21. 2003
  7. ncbi A gene expression atlas of the central nervous system based on bacterial artificial chromosomes
    Shiaoching Gong
    GENSAT Project, Howard Hughes Medical Institute, The Rockefeller University, 1230 York Avenue, Box 260, New York 10021, USA
    Nature 425:917-25. 2003
  8. ncbi A novel transcription factor, T-bet, directs Th1 lineage commitment
    S J Szabo
    Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, Massachusetts 02115, USA
    Cell 100:655-69. 2000
  9. ncbi Control of regulatory T cell development by the transcription factor Foxp3
    Shohei Hori
    Laboratory of Immunopathology, Research Center for Allergy and Immunology, Institute for Physical and Chemical Research, Yokohama 230 0045, Japan
    Science 299:1057-61. 2003
  10. ncbi Mice deficient for p53 are developmentally normal but susceptible to spontaneous tumours
    L A Donehower
    Division of Molecular Virology, Baylor College of Medicine, Houston, Texas 77030
    Nature 356:215-21. 1992

Detail Information

Publications314 found, 100 shown here

  1. pmc Cre reporter strains produced by targeted insertion of EYFP and ECFP into the ROSA26 locus
    S Srinivas
    Department of Genetics and Development, Columbia University, New York, USA
    BMC Dev Biol 1:4. 2001
    ....
  2. ncbi Alzheimer's disease is a synaptic failure
    Dennis J Selkoe
    Center for Neurologic Diseases, Brigham and Women s Hospital, and the Harvard Center for Neurodegeneration and Repair, Boston, MA 02115, USA
    Science 298:789-91. 2002
    ....
  3. ncbi Exon 1 of the HD gene with an expanded CAG repeat is sufficient to cause a progressive neurological phenotype in transgenic mice
    L Mangiarini
    Division of Medical and Molecular Genetics, UMDS, Guy s Hospital, London, United Kingdom
    Cell 87:493-506. 1996
    ..In three lines, the transgene is ubiquitously expressed at both mRNA and protein level. Transgenic mice exhibit a progressive neurological phenotype that exhibits many of the features of HD, including choreiform-..
  4. ncbi Motor neuron degeneration in mice that express a human Cu,Zn superoxide dismutase mutation
    M E Gurney
    Department of Cell and Molecular Biology, Northwestern University Medical School, Chicago, IL 60611
    Science 264:1772-5. 1994
    ..to alanine at position 93--a change that has little effect on enzyme activity--caused motor neuron disease in transgenic mice. The mice became paralyzed in one or more limbs as a result of motor neuron loss from the spinal cord and ..
  5. pmc In vivo analysis of autophagy in response to nutrient starvation using transgenic mice expressing a fluorescent autophagosome marker
    Noboru Mizushima
    Department of Cell Biology, National Institute for Basic Biology, Okazaki 444 8585, Japan
    Mol Biol Cell 15:1101-11. 2004
    ..To understand where and when autophagy occurs in vivo, we have generated transgenic mice systemically expressing GFP fused to LC3, which is a mammalian homologue of yeast Atg8 (Aut7/Apg8) and serves ..
  6. ncbi Triple-transgenic model of Alzheimer's disease with plaques and tangles: intracellular Abeta and synaptic dysfunction
    Salvatore Oddo
    Department of Neurobiology and Behavior, University of California, Irvine, Irvine, California 92697, USA
    Neuron 39:409-21. 2003
    ....
  7. ncbi A gene expression atlas of the central nervous system based on bacterial artificial chromosomes
    Shiaoching Gong
    GENSAT Project, Howard Hughes Medical Institute, The Rockefeller University, 1230 York Avenue, Box 260, New York 10021, USA
    Nature 425:917-25. 2003
    ..The atlas, library of BAC vectors and BAC transgenic mice generated in this screen provide a rich resource that allows a broad array of investigations not previously ..
  8. ncbi A novel transcription factor, T-bet, directs Th1 lineage commitment
    S J Szabo
    Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, Massachusetts 02115, USA
    Cell 100:655-69. 2000
    ..Thus, T-bet initiates Th1 lineage development from naive Thp cells both by activating Th1 genetic programs and by repressing the opposing Th2 programs...
  9. ncbi Control of regulatory T cell development by the transcription factor Foxp3
    Shohei Hori
    Laboratory of Immunopathology, Research Center for Allergy and Immunology, Institute for Physical and Chemical Research, Yokohama 230 0045, Japan
    Science 299:1057-61. 2003
    ..Thus, Foxp3 is a key regulatory gene for the development of regulatory T cells...
  10. ncbi Mice deficient for p53 are developmentally normal but susceptible to spontaneous tumours
    L A Donehower
    Division of Molecular Virology, Baylor College of Medicine, Houston, Texas 77030
    Nature 356:215-21. 1992
    ....
  11. pmc Tau suppression in a neurodegenerative mouse model improves memory function
    K SantaCruz
    Department of Laboratory Medicine and Pathology, University of Minnesota Medical School, Minneapolis, MN 55455, USA
    Science 309:476-81. 2005
    ..Thus, NFTs are not sufficient to cause cognitive decline or neuronal death in this model of tauopathy...
  12. ncbi Efficient recombination in diverse tissues by a tamoxifen-inducible form of Cre: a tool for temporally regulated gene activation/inactivation in the mouse
    Shigemi Hayashi
    Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Avenue, Cambridge, Massachusetts 02138, USA
    Dev Biol 244:305-18. 2002
    ..This inducible Cre system will be a broadly useful tool to modulate gene activity in mouse embryos, adults, and culture systems where temporal control is an important consideration...
  13. ncbi IL-23 and IL-17 in the establishment of protective pulmonary CD4+ T cell responses after vaccination and during Mycobacterium tuberculosis challenge
    Shabaana A Khader
    Trudeau Institute, Saranac Lake, New York 12983, USA
    Nat Immunol 8:369-77. 2007
    ....
  14. ncbi Lineage relationship and protective immunity of memory CD8 T cell subsets
    E John Wherry
    Emory Vaccine Center and Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, GA 30322, USA
    Nat Immunol 4:225-34. 2003
    ..We propose that T(CM) and T(EM) do not necessarily represent distinct subsets, but are part of a continuum in a linear naive --> effector --> T(EM) --> T(CM) differentiation pathway...
  15. ncbi Onset and progression in inherited ALS determined by motor neurons and microglia
    Severine Boillee
    Ludwig Institute for Cancer Research and Departments of Medicine and Neuroscience, University of California at San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA
    Science 312:1389-92. 2006
    ....
  16. ncbi Bone marrow-derived microglia play a critical role in restricting senile plaque formation in Alzheimer's disease
    Alain R Simard
    Laboratory of Molecular Endocrinology, CHUL Research Center and Department of Anatomy and Physiology, Laval University, 2705 Laurier Boul, Quebec G1V 4G2, Canada
    Neuron 49:489-502. 2006
    ..Therapeutic strategies aiming to improve their recruitment could potentially lead to a new powerful tool for the elimination of toxic senile plaques...
  17. ncbi Selective expression of the interleukin 7 receptor identifies effector CD8 T cells that give rise to long-lived memory cells
    Susan M Kaech
    Emory Vaccine Center and Department of Microbiology and Immunology, Emory University School of Medicine, 1510 Clifton Road, Atlanta, Georgia 30322, USA
    Nat Immunol 4:1191-8. 2003
    ..Thus, selective expression of IL-7R identifies memory cell precursors, and this marker may be useful in predicting the number of memory T cells generated after infection or immunization...
  18. pmc A distinct lineage of CD4 T cells regulates tissue inflammation by producing interleukin 17
    Heon Park
    Department of Immunology, University of Washington, Seattle, Washington 98195, USA
    Nat Immunol 6:1133-41. 2005
    ..Thus, IL-17 expression characterizes a unique T helper lineage that regulates tissue inflammation...
  19. ncbi Preferential incorporation of adult-generated granule cells into spatial memory networks in the dentate gyrus
    Nohjin Kee
    Program in Neurosciences and Mental Health, The Hospital for Sick Children, 555 University Avenue, Toronto, Ontario M5G 1X8, Canada
    Nat Neurosci 10:355-62. 2007
    ..This preferential recruitment supports the idea that new neurons make a unique contribution to memory processing in the dentate gyrus...
  20. pmc PERIOD2::LUCIFERASE real-time reporting of circadian dynamics reveals persistent circadian oscillations in mouse peripheral tissues
    Seung Hee Yoo
    Department of Neurobiology and Physiology, Northwestern University, 2205 Tech Drive, Evanston, IL 60208, USA
    Proc Natl Acad Sci U S A 101:5339-46. 2004
    ..These results demonstrate that peripheral tissues express self-sustained, rather than damped, circadian oscillations and suggest the existence of organ-specific synchronizers of circadian rhythms at the cell and tissue level...
  21. ncbi Loss of CTLA-4 leads to massive lymphoproliferation and fatal multiorgan tissue destruction, revealing a critical negative regulatory role of CTLA-4
    E A Tivol
    Department of Pathology, Brigham and Women s Hospital, Boston, Massachusetts, USA
    Immunity 3:541-7. 1995
    ..In the absence of CTLA-4, peripheral T cells are activated, can spontaneously proliferate, and may mediate lethal tissue injury...
  22. pmc Mutant LRRK2(R1441G) BAC transgenic mice recapitulate cardinal features of Parkinson's disease
    Yanping Li
    Department of Neurology and Neurosciences, Weill Medical College of Cornell University, New York, New York, USA
    Nat Neurosci 12:826-8. 2009
    ..These mice provide a valid model of Parkinson's disease and are a resource for the investigation of pathogenesis and therapeutics...
  23. ncbi Microglia in the adult brain arise from Ly-6ChiCCR2+ monocytes only under defined host conditions
    Alexander Mildner
    Institute of Neuropathology, Georg August University, Gottingen, Germany
    Nat Neurosci 10:1544-53. 2007
    ..Our results identify Ly-6C(hi)CCR2(+) monocytes as direct precursors of microglia in the adult brain and establish the importance of local factors in the adult CNS for microglia engraftment...
  24. ncbi SIRT1 transgenic mice show phenotypes resembling calorie restriction
    Laura Bordone
    Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Aging Cell 6:759-67. 2007
    We generated mice that overexpress the sirtuin, SIRT1. Transgenic mice have been generated by knocking in SIRT1 cDNA into the beta-actin locus...
  25. ncbi Disruption of the glucocorticoid receptor gene in the nervous system results in reduced anxiety
    F Tronche
    Molecular Biology of the Cell I, Deutsches Krebsforschungzentrum, Im Neuenheimer Feld 280, 69120 Heidelberg, Germany
    Nat Genet 23:99-103. 1999
    ..Conditional mutagenesis of Gr in the nervous system provides genetic evidence for the importance of Gr signalling in emotional behaviour because mutant animals show an impaired behavioural response to stress and display reduced anxiety...
  26. pmc TDP-43 mutant transgenic mice develop features of ALS and frontotemporal lobar degeneration
    Iga Wegorzewska
    Department of Neurology and Hope Center for Neurological Diseases, Washington University School of Medicine, St Louis, MO 63110, USA
    Proc Natl Acad Sci U S A 106:18809-14. 2009
    ....
  27. ncbi Targeted disruption of the mouse transforming growth factor-beta 1 gene results in multifocal inflammatory disease
    M M Shull
    Department of Molecular Genetics, Biochemistry, and Microbiology, University of Cincinnati College of Medicine, Ohio 45267 0524
    Nature 359:693-9. 1992
    ..TGF-beta 1-deficient mice may be valuable models for human immune and inflammatory disorders, including autoimmune diseases, transplant rejection and graft versus host reactions...
  28. ncbi Localized Igf-1 transgene expression sustains hypertrophy and regeneration in senescent skeletal muscle
    A Musaro
    Cardiovascular Research Center, Massachusetts General Hospital East, Charlestown, Massachusetts, USA
    Nat Genet 27:195-200. 2001
    ..The preservation of muscle architecture and age-independent regenerative capacity through localized mIgf-1 transgene expression suggests clinical strategies for the treatment of age or disease-related muscle frailty...
  29. ncbi Reducing endogenous tau ameliorates amyloid beta-induced deficits in an Alzheimer's disease mouse model
    Erik D Roberson
    Gladstone Institute of Neurological Disease, San Francisco, CA 94158, USA
    Science 316:750-4. 2007
    ..Here, we found that reducing endogenous tau levels prevented behavioral deficits in transgenic mice expressing human amyloid precursor protein, without altering their high Abeta levels...
  30. ncbi Insulin resistance and a diabetes mellitus-like syndrome in mice lacking the protein kinase Akt2 (PKB beta)
    H Cho
    Department of Biology, University of Pennsylvania, Philadelphia, PA 19104, USA
    Science 292:1728-31. 2001
    ..These data establish Akt2 as an essential gene in the maintenance of normal glucose homeostasis...
  31. pmc Altered distributions of Gemini of coiled bodies and mitochondria in motor neurons of TDP-43 transgenic mice
    Xiu Shan
    Department of Pathology, Division of Neuropathology, The Johns Hopkins University School of Medicine, Baltimore, MD 21205 2196, USA
    Proc Natl Acad Sci U S A 107:16325-30. 2010
    ..In concert, our findings indicate that TDP-43 participates in pathways critical for motor neuron physiology, including those that regulate the normal distributions of SMN-associated GEMs in the nucleus and mitochondria in the cytoplasm...
  32. ncbi Age-dependent changes in brain, CSF, and plasma amyloid (beta) protein in the Tg2576 transgenic mouse model of Alzheimer's disease
    T Kawarabayashi
    Mayo Clinic Jacksonville, Jacksonville, Florida 32224, USA
    J Neurosci 21:372-81. 2001
    ..If a similar decline occurs in human plasma, it is possible that measurement of plasma Abeta may be useful as a premorbid biomarker for AD...
  33. pmc TDP-43 transgenic mice develop spastic paralysis and neuronal inclusions characteristic of ALS and frontotemporal lobar degeneration
    Hans Wils
    Department of Molecular Genetics, VIB, B 2610 Antwerpen, Belgium
    Proc Natl Acad Sci U S A 107:3858-63. 2010
    ..These findings suggest that approximately 25-kDa TDP-43 CTFs are noxious to neurons by a gain of aberrant nuclear function...
  34. ncbi CD1d-restricted and TCR-mediated activation of valpha14 NKT cells by glycosylceramides
    T Kawano
    CREST Core Research for Evolutional Science and Technology Project, Japan Science and Technology Corporation JST, 1 8 1 Inohana, Chuo, Chiba 260, Japan
    Science 278:1626-9. 1997
    ..Thus, this lymphocyte shares distinct recognition systems with either T or NK cells...
  35. pmc Wild-type human TDP-43 expression causes TDP-43 phosphorylation, mitochondrial aggregation, motor deficits, and early mortality in transgenic mice
    Ya Fei Xu
    Department of Neuroscience, Mayo Clinic, Jacksonville, Florida 32224, USA
    J Neurosci 30:10851-9. 2010
    ..This TDP-43 transgenic line provides a valuable tool for identifying potential roles of wild-type TDP-43 within the CNS and for studying TDP-43-associated neurotoxicity...
  36. ncbi A mouse Mecp2-null mutation causes neurological symptoms that mimic Rett syndrome
    J Guy
    Wellcome Centre for Cell Biology, Institute of Cell and Molecular Biology, University of Edinburgh, The King s Buildings, Edinburgh, UK
    Nat Genet 27:322-6. 2001
    ....
  37. ncbi Intraneuronal beta-amyloid aggregates, neurodegeneration, and neuron loss in transgenic mice with five familial Alzheimer's disease mutations: potential factors in amyloid plaque formation
    Holly Oakley
    Department of Cell and Molecular Biology, The Feinberg School of Medicine, Northwestern University, Chicago, Illinois 60611, USA
    J Neurosci 26:10129-40. 2006
    ..b>Transgenic mice that express FAD mutant APP and PS1 overproduce Abeta42 and exhibit amyloid plaque pathology similar to that ..
  38. ncbi RAG-2-deficient mice lack mature lymphocytes owing to inability to initiate V(D)J rearrangement
    Y Shinkai
    Howard Hughes Medical Institute, College of Physicians and Surgeons, Columbia University, New York, New York 10032
    Cell 68:855-67. 1992
    ..Because the SCID phenotype was the only obvious abnormality detected in RAG-2 mutant mice, RAG-2 function and V(D)J recombinase activity, per se, are not required for development of cells other than lymphocytes...
  39. ncbi Differential antigen processing by dendritic cell subsets in vivo
    Diana Dudziak
    Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10021, USA
    Science 315:107-11. 2007
    ..This difference in antigen processing is intrinsic to the DC subsets and is associated with increased expression of proteins involved in MHC processing...
  40. ncbi Modification of gene activity in mouse embryos in utero by a tamoxifen-inducible form of Cre recombinase
    P S Danielian
    Department of Molecular and Cellular Biology Harvard University 16 Divinity Avenue, Cambridge Massachusetts 02138 USA
    Curr Biol 8:1323-6. 1998
    ..Induction was ligand dependent, rapid and efficient. The results demonstrate that tamoxifen-inducible recombination can be used to effectively modify gene function in the mouse embryo...
  41. ncbi Formation of neurofibrillary tangles in P301l tau transgenic mice induced by Abeta 42 fibrils
    J Gotz
    Division of Psychiatry Research, University of Zurich, August Forel Strasse 1, 8008 Zurich, Switzerland
    Science 293:1491-5. 2001
    ..Injection of beta-amyloid Abeta42 fibrils into the brains of P301L mutant tau transgenic mice caused fivefold increases in the numbers of NFTs in cell bodies within the amygdala from where neurons ..
  42. pmc MCP-1 contributes to macrophage infiltration into adipose tissue, insulin resistance, and hepatic steatosis in obesity
    Hajime Kanda
    Department of Clinical Molecular Medicine, Kobe University Graduate School of Medicine, Kobe, Japan
    J Clin Invest 116:1494-505. 2006
    ..These findings suggest that an increase in MCP-1 expression in adipose tissue contributes to the macrophage infiltration into this tissue, insulin resistance, and hepatic steatosis associated with obesity in mice...
  43. ncbi Dopaminergic loss and inclusion body formation in alpha-synuclein mice: implications for neurodegenerative disorders
    E Masliah
    Department of Neurosciences, Department of Pathology, University of California San Diego, La Jolla, CA 92093 0624, USA
    Science 287:1265-9. 2000
    To elucidate the role of the synaptic protein alpha-synuclein in neurodegenerative disorders, transgenic mice expressing wild-type human alpha-synuclein were generated...
  44. doi Looking BAC at striatal signaling: cell-specific analysis in new transgenic mice
    Emmanuel Valjent
    INSERM, UMR S 839, 75005 Paris, France
    Trends Neurosci 32:538-47. 2009
    ..The recent development of bacterial artificial chromosome (BAC) transgenic mice expressing a variety of reporters, epitope tagged-proteins or Cre recombinase driven by specific promoters, ..
  45. ncbi Initial sequencing and comparative analysis of the mouse genome
    Robert H Waterston
    Genome Sequencing Center, Washington University School of Medicine, Campus Box 8501, 4444 Forest Park Avenue, St Louis, Missouri 63108, USA
    Nature 420:520-62. 2002
    ....
  46. pmc Regulation of myostatin activity and muscle growth
    S J Lee
    Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 98:9306-11. 2001
    ..To determine the functional significance of these interactions in vivo, we generated transgenic mice expressing high levels of the propeptide, follistatin, or a dominant-negative form of Act RIIB by using a ..
  47. ncbi CX3CR1-mediated dendritic cell access to the intestinal lumen and bacterial clearance
    Jan Hendrik Niess
    Gastrointestinal Unit, Massachusetts General Hospital and Harvard Medical School, 55 Fruit Street, Boston, MA 02114, USA
    Science 307:254-8. 2005
    ..Thus, CX3CR1-dependent processes, which control host interactions of specialized DCs with commensal and pathogenic bacteria, may regulate immunological tolerance and inflammation...
  48. ncbi Fate of the mammalian cardiac neural crest
    X Jiang
    Departments of Biological Sciences and Cell and Neurobiology, Institute for Genetic Medicine, Keck School of Medicine, University of Southern California, IGM 240, Los Angeles, CA 90033, USA
    Development 127:1607-16. 2000
    ....
  49. ncbi Wild-type nonneuronal cells extend survival of SOD1 mutant motor neurons in ALS mice
    A M Clement
    Ludwig Institute for Cancer Research, University of California, 9500 Gilman Drive, La Jolla, CA 92093 0670, USA
    Science 302:113-7. 2003
    ..Normal motor neurons in SOD1 mutant chimeras develop aspects of ALS pathology. Most important, nonneuronal cells that do not express mutant SOD1 delay degeneration and significantly extend survival of mutant-expressing motor neurons...
  50. ncbi Formation of neuronal intranuclear inclusions underlies the neurological dysfunction in mice transgenic for the HD mutation
    S W Davies
    Department of Anatomy and Developmental Biology, University College London, United Kingdom
    Cell 90:537-48. 1997
    ..The appearance in transgenic mice of these inclusions, followed by characteristic morphological change within neuronal nuclei, is strikingly ..
  51. ncbi Osteoprotegerin: a novel secreted protein involved in the regulation of bone density
    W S Simonet
    Department of Molecular Genetics, Amgen Inc, Thousand Oaks, California 91320, USA
    Cell 89:309-19. 1997
    ..In vivo, hepatic expression of OPG in transgenic mice results in a profound yet nonlethal osteopetrosis, coincident with a decrease in later stages of osteoclast ..
  52. pmc Requirement for hippocampal CA3 NMDA receptors in associative memory recall
    Kazu Nakazawa
    Picower Center for Learning and Memory, RIKEN MIT Neuroscience Research Center, Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Science 297:211-8. 2002
    ..These results provide direct evidence for CA3 NMDA receptor involvement in associative memory recall...
  53. ncbi Amyotrophic lateral sclerosis is a distal axonopathy: evidence in mice and man
    Lindsey R Fischer
    Department of Neurology, Emory University School of Medicine, Atlanta, GA 30322, USA
    Exp Neurol 185:232-40. 2004
    ..We conclude that in this widely studied animal model of human ALS, and in this single human case, motor neuron pathology begins at the distal axon and proceeds in a "dying back" pattern...
  54. ncbi Conditional gene targeting in macrophages and granulocytes using LysMcre mice
    B E Clausen
    Institute for Genetics, University of Cologne, Germany
    Transgenic Res 8:265-77. 1999
    ..In contrast, no significant deletion was observed in tail DNA or purified T and B cells. Taken together, LysMcre mice allow for both specific and highly efficient Cre-mediated deletion of loxP-flanked target genes in myeloid cells...
  55. pmc Selective depletion of Foxp3+ regulatory T cells induces a scurfy-like disease
    Katharina Lahl
    Institut fur Medizinische Mikrobiologie, Immunologie und Hygiene, Technische Universitat Munchen, 81675 Munich, Germany
    J Exp Med 204:57-63. 2007
    ..We describe the generation of bacterial artificial chromosome-transgenic mice termed "depletion of regulatory T cell" (DEREG) mice expressing a diphtheria toxin (DT) receptor-enhanced ..
  56. ncbi Extension of murine life span by overexpression of catalase targeted to mitochondria
    Samuel E Schriner
    Department of Genome Sciences, University of Washington, Seattle, WA 91895, USA
    Science 308:1909-11. 2005
    To determine the role of reactive oxygen species in mammalian longevity, we generated transgenic mice that overexpress human catalase localized to the peroxisome, the nucleus, or mitochondria (MCAT)...
  57. ncbi Aberrant excitatory neuronal activity and compensatory remodeling of inhibitory hippocampal circuits in mouse models of Alzheimer's disease
    Jorge J Palop
    Gladstone Institute of Neurological Disease, San Francisco, CA 94158, USA
    Neuron 55:697-711. 2007
    ..Neuronal circuits vulnerable to AD are also affected in human amyloid precursor protein (hAPP) transgenic mice. hAPP mice with high levels of amyloid-beta peptides in the brain develop AD-like abnormalities, including ..
  58. ncbi Interleukin-7 mediates the homeostasis of naïve and memory CD8 T cells in vivo
    K S Schluns
    Department of Medicine, University of Connecticut Health Center, Farmington, CT 06030, USA
    Nat Immunol 1:426-32. 2000
    ..Homeostatic proliferation of memory cells was also partially dependent on IL-7. These results point to IL-7 as a pivotal cytokine in T cell homeostasis...
  59. pmc Skeletal muscle-restricted expression of human SOD1 causes motor neuron degeneration in transgenic mice
    Margaret Wong
    Division of Neuropathology, Department of Pathology, Johns Hopkins University School of Medicine, 558 Ross Building, 720 Rutland Avenue, Baltimore, MD 21205 2196, USA
    Hum Mol Genet 19:2284-302. 2010
    ..The discovery of instigating molecular toxicities or disease progression determinants within skeletal muscle could be very valuable for the development of new effective therapies for the treatment and cure of ALS...
  60. ncbi Bone morphogenetic proteins
    Di Chen
    School of Medicine and Dentistry, Department of Orthopaedics, University of Rochester, Rochester, NY 14642, USA
    Growth Factors 22:233-41. 2004
    ..A significant advancement about the understanding of in vivo functions of BMP ligands, receptors and signaling molecules has been achieved in recent years...
  61. pmc In vivo depletion of CD11c+ dendritic cells abrogates priming of CD8+ T cells by exogenous cell-associated antigens
    Steffen Jung
    Molecular Pathogenesis Program, Skirball Institute of Biomolecular Medicine, New York University School of Medicine, New York 10016, USA
    Immunity 17:211-20. 2002
    ..e., the sensitization of the immune system for cell-associated antigens. DC-depleted mice fail to mount CTL responses to infection with the intracellular bacterium Listeria monocytogenes and the rodent malaria parasite Plasmodium yoelii...
  62. ncbi Early-onset amyloid deposition and cognitive deficits in transgenic mice expressing a double mutant form of amyloid precursor protein 695
    M A Chishti
    Centre for Research in Neurodegenerative Diseases, The Department of Laboratory Medicine, Division of Neurology, University Health Network, University of Toronto, Toronto, Ontario M5S 3H2, Canada
    J Biol Chem 276:21562-70. 2001
    ..The Tg mice described here suggest a potential to investigate aspects of Alzheimer's disease pathogenesis, prophylaxis, and therapy within short time frames...
  63. ncbi Effector and memory CD8+ T cell fate coupled by T-bet and eomesodermin
    Andrew M Intlekofer
    Abramson Family Cancer Research Institute and Department of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Nat Immunol 6:1236-44. 2005
    ..Therefore, these key transcription factors link the long-term renewal of memory CD8(+) T cells to their characteristic effector potency...
  64. ncbi A beta peptide vaccination prevents memory loss in an animal model of Alzheimer's disease
    D Morgan
    Department of Pharmacology, University of South Florida, Tampa 33612, USA
    Nature 408:982-5. 2000
    ..Here we show that vaccination with A beta protects transgenic mice from the learning and age-related memory deficits that normally occur in this mouse model for Alzheimer's ..
  65. pmc Loss of Apc in vivo immediately perturbs Wnt signaling, differentiation, and migration
    Owen J Sansom
    School of Biosciences, University of Cardiff, Cardiff CF10 3US, Wales
    Genes Dev 18:1385-90. 2004
    ..Critically, for the first time we confirm a series of Wnt target molecules in an in vivo setting and also identify a series of new candidate targets within the same setting...
  66. ncbi Regulation of cerebral cortical size by control of cell cycle exit in neural precursors
    Anjen Chenn
    Department of Pathology, Brigham and Women s Hospital, Boston, MA 02115, USA
    Science 297:365-9. 2002
    b>Transgenic mice expressing a stabilized beta-catenin in neural precursors develop enlarged brains with increased cerebral cortical surface area and folds resembling sulci and gyri of higher mammals...
  67. ncbi TGFbeta in the context of an inflammatory cytokine milieu supports de novo differentiation of IL-17-producing T cells
    Marc Veldhoen
    Division of Molecular Immunology, MRC National Institute for Medical Research, Mill Hill, London NW7 1AA, United Kingdom
    Immunity 24:179-89. 2006
    ..Our data indicate that, in the presence of IL-6, TGFbeta1 subverts Th1 and Th2 differentiation for the generation of IL-17-producing T cells...
  68. ncbi Severe hypercholesterolemia and atherosclerosis in apolipoprotein E-deficient mice created by homologous recombination in ES cells
    A S Plump
    Laboratory of Biochemical Genetics and Metabolism, The Rockefeller University, New York, New York 10021 6399
    Cell 71:343-53. 1992
    ..apoE-deficient mice are a promising small animal model to help understand the role of apoE in vivo and the genetic and environmental determinants of atherosclerosis...
  69. pmc Astrocytes expressing ALS-linked mutated SOD1 release factors selectively toxic to motor neurons
    Makiko Nagai
    Department of Neurology, Columbia University, 710 West 168th Street, New York, New York 10032, USA
    Nat Neurosci 10:615-22. 2007
    ..Identification of the astrocyte-derived soluble factor(s) may have far-reaching implications for ALS from both a pathogenic and therapeutic standpoint...
  70. ncbi Inhibition of mTOR induces autophagy and reduces toxicity of polyglutamine expansions in fly and mouse models of Huntington disease
    Brinda Ravikumar
    Department of Medical Genetics, Cambridge Institute for Medical Research, Wellcome MRC Building, Addenbrooke s Hospital, Hills Road, Cambridge CB2 2XY, UK
    Nat Genet 36:585-95. 2004
    ..we show that mammalian target of rapamycin (mTOR) is sequestered in polyglutamine aggregates in cell models, transgenic mice and human brains...
  71. ncbi Abeta immunotherapy leads to clearance of early, but not late, hyperphosphorylated tau aggregates via the proteasome
    Salvatore Oddo
    Institute for Brain Aging and Dementia, University of California, Irvine, Irvine, CA 92697, USA
    Neuron 43:321-32. 2004
    ..These findings indicate that Abeta immunization may be useful for clearing both hallmark lesions of AD, provided that intervention occurs early in the disease course...
  72. doi Rapid restoration of cognition in Alzheimer's transgenic mice with 8-hydroxy quinoline analogs is associated with decreased interstitial Abeta
    Paul A Adlard
    Oxidation Biology Laboratory, The Mental Health Research Institute of Victoria, Parkville, Victoria 3052, Australia
    Neuron 59:43-55. 2008
    ..The speed of recovery of the animals underscores the acutely reversible nature of the cognitive deficits associated with transgenic models of AD...
  73. pmc Microglial dysfunction and defective beta-amyloid clearance pathways in aging Alzheimer's disease mice
    Suzanne E Hickman
    Center for Immunology and Inflammatory Diseases, Division of Rheumatology, Allergy, and Immunology, and Division of Infectious Diseases, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts 02129
    J Neurosci 28:8354-60. 2008
    ..Antiinflammatory therapy for AD should take this dichotomous microglial role into consideration...
  74. ncbi Experience and activity-dependent maturation of perisomatic GABAergic innervation in primary visual cortex during a postnatal critical period
    Bidisha Chattopadhyaya
    Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
    J Neurosci 24:9598-611. 2004
    ..Using bacterial artificial chromosome transgenic mice that label a defined class of perisomatic synapses with green fluorescent protein, here we show that ..
  75. pmc In vivo light-induced activation of neural circuitry in transgenic mice expressing channelrhodopsin-2
    Benjamin R Arenkiel
    Howard Hughes Medical Institute, Duke University Medical Center, Durham, NC 27710, USA
    Neuron 54:205-18. 2007
    ..Here, we report the generation of transgenic mice that express a ChR2-YFP fusion protein in the CNS for in vivo activation and mapping of neural circuits...
  76. ncbi Development of lupus-like autoimmune diseases by disruption of the PD-1 gene encoding an ITIM motif-carrying immunoreceptor
    H Nishimura
    Department of Medical Chemistry, Faculty of Medicine, Kyoto University, Japan
    Immunity 11:141-51. 1999
    ..Introduction of a PD-1 null mutation into the 2C-TCR (anti-H-2Ld) transgenic mice of the H-2(b/d) background resulted in the chronic and systemic graft-versus-host-like disease...
  77. ncbi APP processing and synaptic function
    Flavio Kamenetz
    Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
    Neuron 37:925-37. 2003
    ..Disruption of this feedback system could contribute to disease progression in AD...
  78. pmc Decreased nuclear beta-catenin, tau hyperphosphorylation and neurodegeneration in GSK-3beta conditional transgenic mice
    J J Lucas
    Centro de Biologia Molecular Severo Ochoa, CSIC Universidad Autonoma de Madrid, Departamento de Morfologia, Facultad de Medicina, Universidad Autonoma de Madrid, 28049 Madrid, Spain
    EMBO J 20:27-39. 2001
    ..By using the tet-regulated system we have produced conditional transgenic mice overexpressing GSK-3beta in the brain during adulthood while avoiding perinatal lethality due to embryonic ..
  79. ncbi Proapoptotic Bcl-2 relative Bim required for certain apoptotic responses, leukocyte homeostasis, and to preclude autoimmunity
    P Bouillet
    The Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria 3050, Australia
    Science 286:1735-8. 1999
    ..Thus, Bim is required for hematopoietic homeostasis and as a barrier to autoimmunity. Moreover, particular death stimuli appear to activate apoptosis through distinct BH3-only proteins...
  80. ncbi In vivo enhancer analysis of human conserved non-coding sequences
    Len A Pennacchio
    US Department of Energy Joint Genome Institute, Walnut Creek, California 94598, USA
    Nature 444:499-502. 2006
    ..The testing of the top predictions in transgenic mice resulted in a threefold enrichment for sequences with forebrain enhancer activity...
  81. ncbi Interleukin-2 receptor alpha chain regulates the size and content of the peripheral lymphoid compartment
    D M Willerford
    Howard Hughes Medical Institute, Department of Pediatrics, Children s Hospital, Boston, Massachusetts 02115, USA
    Immunity 3:521-30. 1995
    ..Thus, IL-2R alpha is essential for regulation of both the size and content of the peripheral lymphoid compartment, probably by influencing the balance between clonal expansion and cell death following lymphocyte activation...
  82. pmc Neurons arise in the basal neuroepithelium of the early mammalian telencephalon: a major site of neurogenesis
    Wulf Haubensak
    Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, D 01307 Dresden, Germany
    Proc Natl Acad Sci U S A 101:3196-201. 2004
    ..Our observations reconcile previous data on the origin and lineage of CNS neurons and show that basal, rather than apical, progenitors are the major source of the neurons of the mammalian neocortex...
  83. ncbi Leptin receptor signaling in POMC neurons is required for normal body weight homeostasis
    Nina Balthasar
    Department of Medicine, Division of Endocrinology, Beth Israel Deaconess Medical Center, Harvard Medical School, 99 Brookline Avenue RN, Boston, MA 02215, USA
    Neuron 42:983-91. 2004
    ..In summary, leptin receptors on POMC neurons are required but not solely responsible for leptin's regulation of body weight homeostasis...
  84. ncbi Osteoblastic cells regulate the haematopoietic stem cell niche
    L M Calvi
    Endocrine Unit, Department of Medicine, Center for Human Genetics and Molecular Pediatric Disease, University of Rochester School of Medicine, Rochester, New York 14642, USA
    Nature 425:841-6. 2003
    ..Niche constituent cells or signalling pathways provide pharmacological targets with therapeutic potential for stem-cell-based therapies...
  85. ncbi Early determination and long-term persistence of adult-generated new neurons in the hippocampus of mice
    Gerd Kempermann
    Max Delbruck Center for Molecular Medicine, Berlin Buch, Robert Rossle Str 10, 13125 Berlin, Germany
    Development 130:391-9. 2003
    ..In transgenic mice expressing enhanced green fluorescent protein under the nestin promoter none of the BrdU/nestin-positive ..
  86. doi The key roles of elongases and desaturases in mammalian fatty acid metabolism: Insights from transgenic mice
    Herve Guillou
    Integrative Toxicology and Metabolism, Pôle de Toxicologie Alimentaire, Laboratoire de Pharmacologie et Toxicologie, Institut National de la Recherche Agronomique INRA UR66, Toulouse Cedex 3, France
    Prog Lipid Res 49:186-99. 2010
    ....
  87. ncbi Germline transmission and tissue-specific expression of transgenes delivered by lentiviral vectors
    Carlos Lois
    Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA
    Science 295:868-72. 2002
    Single-cell mouse embryos were infected in vitro with recombinant lentiviral vectors to generate transgenic mice carrying the green fluorescent protein (GFP) gene driven by a ubiquitously expressing promoter...
  88. ncbi The transcriptional repressor Snail promotes mammary tumor recurrence
    Susan E Moody
    Department of Cancer Biology, Abramson Family Cancer Research Institute, University of Pennsylvania School of Medicine, Philadelphia, 19104, USA
    Cancer Cell 8:197-209. 2005
    ..In aggregate, our observations strongly implicate Snail in the process of breast cancer recurrence...
  89. pmc Progressive accumulation of amyloid-beta oligomers in Alzheimer's disease and in amyloid precursor protein transgenic mice is accompanied by selective alterations in synaptic scaffold proteins
    Emiley Pham
    Department of Neurosciences, University of California, San Diego, La Jolla, CA 92093 0624, USA
    FEBS J 277:3051-67. 2010
    ..Similarly, in transgenic mice expressing high levels of neuronal amyloid precursor protein, amyloid-beta co-immunoprecipitated with ..
  90. doi Oscillations in notch signaling regulate maintenance of neural progenitors
    Hiromi Shimojo
    Institute for Virus Research, Kyoto University, and Japan Science and Technology Agency, CREST, Kyoto 606 8507, Japan
    Neuron 58:52-64. 2008
    ..These results suggest that Hes1 oscillation regulates Ngn2 and Dll1 oscillations, which in turn lead to maintenance of neural progenitors by mutual activation of Notch signaling...
  91. pmc Bmi1 is expressed in vivo in intestinal stem cells
    Eugenio Sangiorgi
    Howard Hughes Medical Institute and Department of Human Genetics, University of Utah School of Medicine, Salt Lake City, Utah 84112, USA
    Nat Genet 40:915-20. 2008
    ..Unexpectedly, the distribution of Bmi1-expressing stem cells along the length of the small intestine suggested that mammals use more than one molecularly distinguishable adult stem cell subpopulation to maintain organ homeostasis...
  92. doi Schizophrenia-related neural and behavioral phenotypes in transgenic mice expressing truncated Disc1
    Sanbing Shen
    School of Medical Sciences, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen AB252ZD, United Kingdom
    J Neurosci 28:10893-904. 2008
    ..To model this mutation in mice, we have generated Disc1(tr) transgenic mice expressing 2 copies of truncated Disc1 encoding the first 8 exons using a bacterial artificial chromosome (..
  93. ncbi Neuronal alpha-synucleinopathy with severe movement disorder in mice expressing A53T human alpha-synuclein
    Benoit I Giasson
    Center for Neurodegenerative Disease Research, Department of Pathology and Laboratory Medicine, 3600 Spruce Street, University of Pennsylvania School of Medicine, Philadelphia 19104, USA
    Neuron 34:521-33. 2002
    ..To explore this issue, we generated transgenic mice expressing wild-type and A53T human alpha-synuclein in CNS neurons...
  94. ncbi Targeting small Abeta oligomers: the solution to an Alzheimer's disease conundrum?
    W L Klein
    Northwestern University Institute for Neuroscience and Dept of Neurobiology and Physiology, Northwestern University, 2153 N Campus Drive, Evanston, IL 60208, USA
    Trends Neurosci 24:219-24. 2001
    ..Immuno-neutralization of soluble Abeta-derived toxins might be the key to optimizing AD vaccines that are now on the horizon...
  95. pmc Myelin oligodendrocyte glycoprotein-specific T cell receptor transgenic mice develop spontaneous autoimmune optic neuritis
    Estelle Bettelli
    Center for Neurologic Diseases, Brigham and Women s Hospital and Harvard Medical School, Boston, MA 02115, USA
    J Exp Med 197:1073-81. 2003
    ..We have generated novel T cell receptor (TCR) transgenic mice specific for myelin oligodendrocyte glycoprotein (MOG)...
  96. pmc Direct expansion of functional CD25+ CD4+ regulatory T cells by antigen-processing dendritic cells
    Sayuri Yamazaki
    Laboratory of Cellular Physiology and Immunology, The Rockefeller University, New York, NY 10021, USA
    J Exp Med 198:235-47. 2003
    ..The capacity to expand CD25+ CD4+ T cells provides DCs with an additional mechanism to regulate autoimmunity and other immune responses...
  97. pmc Astrocytes as determinants of disease progression in inherited amyotrophic lateral sclerosis
    Koji Yamanaka
    Ludwig Institute for Cancer Research and Department of Medicine and Neuroscience, University of California at San Diego, 9500 Gilman Drive, La Jolla, California 92093 0670, USA
    Nat Neurosci 11:251-3. 2008
    ..These findings demonstrate that mutant astrocytes are viable targets for therapies for slowing the progression of non-cell autonomous killing of motor neurons in ALS...
  98. pmc Ablation of hippocampal neurogenesis impairs contextual fear conditioning and synaptic plasticity in the dentate gyrus
    Michael D Saxe
    Center for Neurobiology and Behavior, Columbia University, New York, NY 10032, USA
    Proc Natl Acad Sci U S A 103:17501-6. 2006
    ..These data show that new hippocampal neurons can be preferentially recruited over mature granule cells in vitro and may provide a framework for how this small cell population can influence behavior...
  99. pmc Inhibition of glycogen synthase kinase-3 by lithium correlates with reduced tauopathy and degeneration in vivo
    Wendy Noble
    Center for Dementia Research, Nathan S Kline Institute, New York University, 140 Old Orangeburg Road, Orangeburg, NY 10962, USA
    Proc Natl Acad Sci U S A 102:6990-5. 2005
    ..investigate whether kinase inhibition can reduce tauopathy and the degeneration associated with it in vivo, transgenic mice overexpressing mutant human tau were treated with the glycogen synthase kinase-3 (GSK-3) inhibitor lithium ..
  100. ncbi ATP mediates rapid microglial response to local brain injury in vivo
    Dimitrios Davalos
    Molecular Neurobiology Program, Department of Physiology and Neuroscience, New York University School of Medicine, 540 First Avenue, New York, New York 10016, USA
    Nat Neurosci 8:752-8. 2005
    ..Thus, extracellular ATP regulates microglial branch dynamics in the intact brain, and its release from the damaged tissue and surrounding astrocytes mediates a rapid microglial response towards injury...
  101. pmc IL-17A and IL-17F do not contribute vitally to autoimmune neuro-inflammation in mice
    Stefan Haak
    Neuroimmunology Division, Institute of Experimental Immunology, Department of Pathology, University Hospital Zurich, Zurich, Switzerland
    J Clin Invest 119:61-9. 2009
    ..To study the impact of IL-17A on CNS inflammation, we generated transgenic mice in which high levels of expression of IL-17A could be initiated after Cre-mediated recombination...

Research Grants81

  1. Cyclohexanehexol Therapy in Transgenic Models of Alzheimer's Disease
    Alpaslan Dedeoglu; Fiscal Year: 2009
    ..of the NSAID flurbiprofen that lowers A42 but lacks cyclooxygenase inhibition reduces A burden in AD transgenic mice and is currently being tested in a phase III AD clinical trial...
  2. Fibrotic Sequelae of Childhood Renal Disease
    Agnes Fogo; Fiscal Year: 2007
    ..Am J Physiol Renal Physiol, 278: F 339-351, 2000. Miyazaki Y, Oshima K, Fogo A, Hogan BL, Ichikawa I: Bone morphogenetic protein 4 regulates the budding ..
  3. AID-mediated genetic instability in BCR-ABL1-transformed B cell lineage leukemia
    MARKUS MUSCHEN; Fiscal Year: 2009
    ..compare development of Imatinib-resistance in Aid-/- and Aid-wildtype leukemias developing in BCR-ABL1 p190-transgenic mice. Aim 2: Contribution of AID-induced mutations to progression of CML into lymphoid blast crisis: To clarify to ..
  4. Gamma-Synuclein-Mediated Regulation of Norepinephrine Transporter
    Anita Sidhu; Fiscal Year: 2010
    ..Syn overexpressing transgenic mice, as well as in .-Syn knock-out mice...
  5. Functional cloning of S. mansoni egg-specific Th2 cells
    Markus Mohrs; Fiscal Year: 2007
    ..This is due to the fact that helminth-specific T cell receptors (TCR) have not been isolated to generate TCR transgenic mice. This situation is compounded by the fact that as of to date it is not possible to engineer antigen-..
  6. PATHOGENESIS, IMMUNODIAGNOSIS AND THERAPY OF HCC
    Jack Wands; Fiscal Year: 2007
    ..of Fas receptor expression, and/or induces the loss of pro-apoptotic mechanisms observed in the single IRS-1 transgenic mice. Specific Aim #2 - Further characterize a critical downstream effector gene (AAH) upregulated through ..
  7. MOLECULAR MECHANISMS OF MHC LINKED SUSCEPTIBILITY
    Kai Wucherpfennig; Fiscal Year: 2001
    ..b>Transgenic mice that express the PV-associated DQalpha and DQbeta genes (DQA1*0101, DQB1*0503) will be generated as an animal ..
  8. Molecular Pathphysiology of FSHD muscular dystrophy via genome-wide approaches
    Yi Wen Chen; Fiscal Year: 2009
    ..In aim 4C, we will systematically deliver vivo-morpholinos against Pitx1 to the Pitx1 transgenic mice. The goal is to determine if the vivo-morpholinos can suppress the transgene and reverse the phenotype, as ..
  9. Androgen receptor targeted vaccines for prostate cancer
    Douglas G McNeel; Fiscal Year: 2010
    ..This will be evaluated in HLA-A2 transgenic mice, and in an HLA-A2-expressing transgenic mouse model of prostate cancer...
  10. Role of HLA class II genes in demyelination
    Chella S David; Fiscal Year: 2010
    ..which HLA-DR and -DQ genes contribute to susceptibility and resistance to MS, we generated single and double transgenic mice expressing HLA class II genes and lacking endogenous mouse class II genes...
  11. Activators of the Pyrophosphatase Activity of Alkaline Phosphatase
    Jose Luis Millan; Fiscal Year: 2007
    ..We recently tested this axis by surmising that transgenic mice over-expressing TNAP might be able to achieve tissular expression of TNAP sufficiently high to be able to ..
  12. Single Cross-Bridge Kinetics in Transgenic Mouse Hearts Expressing FHC Mutations
    Danuta Szczesna Cordary; Fiscal Year: 2010
    ..on skinned papillary muscle fibers as well as force and calcium transients on intact muscle fibers from transgenic mice (Aim 2A)...
  13. Mechanism of gallbladder tumorigenesis in BK5.erbB2 mice
    Kaoru Kiguchi; Fiscal Year: 2007
    ..We recently developed the BK5.erbB2 transgenic mice where expression of a rat erbB2 cDNA is targeted to the basal layer of multiple epithelial tissues, including ..
  14. Cyclohexanehexol Therapy in Transgenic Models of Alzheimer's Disease
    Alpaslan Dedeoglu; Fiscal Year: 2010
    ..of the NSAID flurbiprofen that lowers A42 but lacks cyclooxygenase inhibition reduces A burden in AD transgenic mice and is currently being tested in a phase III AD clinical trial...
  15. Humanized Transgenic Mice Reproduce the Disease Evolution in Severe RA
    Grete Sonderstrup; Fiscal Year: 2010
    Humanized transgenic mice reproduce the disease evolution in severe RA Rheumatoid arthritis (RA) is a common autoimmune disease in humans genetically associated with specific HLA class II alleles...
  16. A MODEL FOR SICKLE CELL DISEASE & GENETIC THERAPIES
    Tim Townes; Fiscal Year: 1999
    In the past few years several groups have produced transgenic mice t hat synthesize high levels of human sickle hemoglobin (Hb S)...
  17. The Role of Cul4A in Genome Stability and Cancer Development
    Liang You; Fiscal Year: 2010
    ..We have generated a Cul4A transgenic mouse model. We are now ready to use the Cul4A transgenic mice to test our hypothesis that Cul4A is an oncogene...
  18. CORRECTION OF T-CELL AGING IN TRANSGENIC MICE
    JOHN MOUNTZ; Fiscal Year: 1993
    ..This proposal will study the fas apoptosis gene as a candidate LAG in CD2-fas transgenic mice. The fas apoptosis gene has recently been found to be mutated in autoimmune lprcg mice, and defective ..
  19. TRANSGENIC MICE, AGING, CANCER, AND OXIDATIVE DAMAGE
    Arlan Richardson; Fiscal Year: 2001
    ..In this proposal, transgenic mice that show reduced expression of either Mn-SOD (Sod2+/-) or both Mn-SOD and the selenium-dependent glutathione ..
  20. IMMUNOGENETICS OF COLLAGEN INDUCED ARTHRITIS IN MICE
    Chella David; Fiscal Year: 2007
    ..We generated transgenic mice expressing human class II genes...
  21. IMMUNOGENETICS OF COLLAGEN INDUCED ARTHRITIS
    Chella David; Fiscal Year: 2000
    ..we accomplished the monumental task of generating a humanized mouse model for polyarthritis using our HLA-DQ8 transgenic mice. In this proposal we will build this humanized mouse model to obtain all the critical information to ..
  22. Mutant DISC 1 and Abnormal Neurodevelopment in Mental Illness
    Mikhail Pletnikov; Fiscal Year: 2009
    ..in levels and distribution of endogenous Disc1 and Its interacting proteins, Lis1 and Ndel1, in hDISC1 transgenic mice. Significance: The application is anticipated to advance our understanding of when, where and how mutant ..
  23. Mutant DISC 1 and Abnormal Neurodevelopment in Mental Illness
    Mikhail V Pletnikov; Fiscal Year: 2010
    ..in levels and distribution of endogenous Disc1 and Its interacting proteins, Lis1 and Ndel1, in hDISC1 transgenic mice. Significance: The application is anticipated to advance our understanding of when, where and how mutant ..
  24. AGGRECAN-hG1 AND LP TRANSGENIC MICE AS MODELS OF OSTEOARTHRITIS (OA)
    Tibor Glant; Fiscal Year: 2007
    ..We have generated transgenic mice overexpressing either LP or G1 domain (hG1) of human aggrecan in cartilage...
  25. Mechanisms of Acquired Central Transplantation Tolerance
    James Markmann; Fiscal Year: 2003
    ..this proposal, we will dissect the mechanisms of acquired central tolerance using a line of T cell receptor transgenic mice (TS1) in which a high percentage of CD4 T cells have class II restricted specificity for a dominant epitope (..
  26. MECHANISMS OF AUTOIMMUNE ARTHRITIS
    EDWARD ROSLONIEC; Fiscal Year: 2001
    ..In previously published studies and preliminary data, the investigators describe the production of transgenic mice expressing chimeric MHC class II molecules in which the peptide binding domain of the beta subunit is derived ..
  27. Mouse Models of ErbB-2 and Cyclin D1 in Prostate Cancer
    Christopher Albanese; Fiscal Year: 2002
    ..We propose to test this hypothesis directly using transgenic mice. Determining the role of ErbB-2 and cyclin D1 in prostate cancer progression and determining the mechanisms ..
  28. Keratin proteins and non-melanoma skin tumors
    PIERRE COULOMBE; Fiscal Year: 2007
    ..do so by mating previously characterized K17 null mice, in which the K17 protein is completely missing, with transgenic mice that constitutively express Gli2 in progenitor keratinocytes of the skin, and which reproducibly develop BCC ..
  29. GENOTOXICITY IN CYP1A2-DEFICIENT TRANSGENIC MICE
    Daniel Nebert; Fiscal Year: 1993
    ..This is particularly important, since recent studies have demonstrated a trimodal distribution and >60-fold interindividual variation in the constitutive expression of CYP1A2 in human liver...
  30. Proteoglycans/Glycosaminoglycans in APP Transgenic Mice
    ALAN SNOW; Fiscal Year: 2004
    ..and 2) Detect and Quantify Heparan Sulfate GAGs Derived from Biological Fluids of Human AD Patients and APP Transgenic Mice, and Determine Their Utility as a Diagnostic Marker for AD...