genetically modified plants

Summary

Summary: PLANTS, or their progeny, whose GENOME has been altered by GENETIC ENGINEERING.

Top Publications

  1. ncbi GATEWAY vectors for Agrobacterium-mediated plant transformation
    Mansour Karimi
    Dept of Plant Systems Biology, Flanders Interuniversity Institute for Biotechnology (VIB, Ghent University, K.L. Ledeganckstraat 35, B-9000, Gent, Belgium
    Trends Plant Sci 7:193-5. 2002
  2. ncbi A gateway cloning vector set for high-throughput functional analysis of genes in planta
    Mark D Curtis
    Institute of Plant Biology and Zurich Basel Plant Science Centre, University of Zurich, Zollikerstrasse 107, Ch 8008 Zurich, Switzerland
    Plant Physiol 133:462-9. 2003
  3. ncbi FT protein movement contributes to long-distance signaling in floral induction of Arabidopsis
    Laurent Corbesier
    Max Planck Institute for Plant Breeding Research, Carl von Linne Weg 10, D 50829 Cologne, Germany
    Science 316:1030-3. 2007
  4. ncbi An enhanced transient expression system in plants based on suppression of gene silencing by the p19 protein of tomato bushy stunt virus
    Olivier Voinnet
    The Sainsbury Laboratory, John Innes Centre, Colney Lane, Norwich NR4 7UH, UK
    Plant J 33:949-56. 2003
  5. ncbi ETHYLENE RESPONSE FACTOR1 integrates signals from ethylene and jasmonate pathways in plant defense
    Oscar Lorenzo
    Departamento de Genética Molecular de Plantas, Centro Nacional de Biotecnología Consejo Superior de Investigaciones Científicas, Campus Universidad Autonoma, 28049 Madrid, Spain
    Plant Cell 15:165-78. 2003
  6. ncbi Regulators of PP2C phosphatase activity function as abscisic acid sensors
    Yue Ma
    Lehrstuhl fur Botanik, Technische Universitat Munchen, Am Hochanger 4, D 85354 Freising, Germany
    Science 324:1064-8. 2009
  7. ncbi Role of arabidopsis MYC and MYB homologs in drought- and abscisic acid-regulated gene expression
    H Abe
    Biological Resources Division, Japan International Research Center for Agricultural Sciences JIRCAS, Ibaraki, Japan
    Plant Cell 9:1859-68. 1997
  8. ncbi A pair of related genes with antagonistic roles in mediating flowering signals
    Y Kobayashi
    Department of Botany, Graduate School of Science, Kyoto University, Sakyo ku, Kyoto 606 8502, Japan
    Science 286:1960-2. 1999
  9. ncbi Isolation and functional analysis of Arabidopsis stress-inducible NAC transcription factors that bind to a drought-responsive cis-element in the early responsive to dehydration stress 1 promoter
    Lam Son Phan Tran
    Biological Resources Division, Japan International Research Center for Agricultural Sciences, Tsukuba, Ibaraki 305 8686, Japan
    Plant Cell 16:2481-98. 2004
  10. ncbi OsDREB genes in rice, Oryza sativa L., encode transcription activators that function in drought-, high-salt- and cold-responsive gene expression
    Joseph G Dubouzet
    Biological Resources Division, Japan International Research Center for Agricultural Sciences (JIRCAS, 1-1 Ohwashi, Tsukuba, Ibaraki 305-8686, Japan
    Plant J 33:751-63. 2003

Research Grants

  1. Expression of Human Therapeutic Proteins in Chloroplasts
    Henry Daniell; Fiscal Year: 2005
  2. Artemisinin Biosynthesis: Control of Transcription
    Pamela Weathers; Fiscal Year: 2007
  3. Rhabdovirus phosphoproteins: RNA silencing and complex formation
    Michael Goodin; Fiscal Year: 2007
  4. Mammalian Lactoferrin Receptors: Structure and Function
    Bo Lonnerdal; Fiscal Year: 2007
  5. Expression of Human Therapeutic Proteins in Transgenic Tobacco Chloroplasts
    Henry Daniell; Fiscal Year: 2006
  6. Expression of Human Therapeutic Proteins in Transgenic Tobacco Chloroplasts
    Henry Daniell; Fiscal Year: 2010
  7. Expression of Human Therapeutic Proteins in Transgenic Tobacco Chloroplasts
    Henry Daniell; Fiscal Year: 2009
  8. Drosophila Transgenic RNAi Resource Project
    Norbert Perrimon; Fiscal Year: 2009
  9. Functional Genomic analysis using RNAi screen in Drosophila
    Norbert Perrimon; Fiscal Year: 2010
  10. DOSAGE COMPENSATION IN DROSOPHILA
    James A Birchler; Fiscal Year: 2010

Detail Information

Publications245 found, 100 shown here

  1. ncbi GATEWAY vectors for Agrobacterium-mediated plant transformation
    Mansour Karimi
    Dept of Plant Systems Biology, Flanders Interuniversity Institute for Biotechnology (VIB, Ghent University, K.L. Ledeganckstraat 35, B-9000, Gent, Belgium
    Trends Plant Sci 7:193-5. 2002
    ..Because these vectors are generally large, cloning can be time-consuming and laborious. Recently, the GATEWAY conversion technology has provided a fast and reliable alternative to the cloning of sequences into large acceptor plasmids...
  2. ncbi A gateway cloning vector set for high-throughput functional analysis of genes in planta
    Mark D Curtis
    Institute of Plant Biology and Zurich Basel Plant Science Centre, University of Zurich, Zollikerstrasse 107, Ch 8008 Zurich, Switzerland
    Plant Physiol 133:462-9. 2003
    ..All vectors were derived from pCambia T-DNA cloning vectors, with the exception of a chemically inducible vector, for Agrobacterium sp.-mediated transformation of a wide range of plant species...
  3. ncbi FT protein movement contributes to long-distance signaling in floral induction of Arabidopsis
    Laurent Corbesier
    Max Planck Institute for Plant Breeding Research, Carl von Linne Weg 10, D 50829 Cologne, Germany
    Science 316:1030-3. 2007
    ..Finally, we provide evidence that FT does not activate an intermediate messenger in leaves. We conclude that FT protein acts as a long-distance signal that induces Arabidopsis flowering...
  4. ncbi An enhanced transient expression system in plants based on suppression of gene silencing by the p19 protein of tomato bushy stunt virus
    Olivier Voinnet
    The Sainsbury Laboratory, John Innes Centre, Colney Lane, Norwich NR4 7UH, UK
    Plant J 33:949-56. 2003
    ....
  5. ncbi ETHYLENE RESPONSE FACTOR1 integrates signals from ethylene and jasmonate pathways in plant defense
    Oscar Lorenzo
    Departamento de Genética Molecular de Plantas, Centro Nacional de Biotecnología Consejo Superior de Investigaciones Científicas, Campus Universidad Autonoma, 28049 Madrid, Spain
    Plant Cell 15:165-78. 2003
    ....
  6. ncbi Regulators of PP2C phosphatase activity function as abscisic acid sensors
    Yue Ma
    Lehrstuhl fur Botanik, Technische Universitat Munchen, Am Hochanger 4, D 85354 Freising, Germany
    Science 324:1064-8. 2009
    ..Other RCARs also mediated ABA-dependent regulation of ABI1 and ABI2, consistent with a combinatorial assembly of receptor complexes...
  7. ncbi Role of arabidopsis MYC and MYB homologs in drought- and abscisic acid-regulated gene expression
    H Abe
    Biological Resources Division, Japan International Research Center for Agricultural Sciences JIRCAS, Ibaraki, Japan
    Plant Cell 9:1859-68. 1997
    ..These results indicate that both the rd22BP1 (MYC) and ATMYB2 (MYB) proteins function as transcriptional activators in the dehydration- and ABA-inducible expression of the rd22 gene...
  8. ncbi A pair of related genes with antagonistic roles in mediating flowering signals
    Y Kobayashi
    Department of Botany, Graduate School of Science, Kyoto University, Sakyo ku, Kyoto 606 8502, Japan
    Science 286:1960-2. 1999
    ..FT acts in part downstream of CO and mediates signals for flowering in an antagonistic manner with its homologous gene, TERMINAL FLOWER1 (TFL1)...
  9. ncbi Isolation and functional analysis of Arabidopsis stress-inducible NAC transcription factors that bind to a drought-responsive cis-element in the early responsive to dehydration stress 1 promoter
    Lam Son Phan Tran
    Biological Resources Division, Japan International Research Center for Agricultural Sciences, Tsukuba, Ibaraki 305 8686, Japan
    Plant Cell 16:2481-98. 2004
    ..However, erd1 was not upregulated in the transgenic plants. Other interacting factors may be necessary for the induction of erd1 in Arabidopsis under stress conditions...
  10. ncbi OsDREB genes in rice, Oryza sativa L., encode transcription activators that function in drought-, high-salt- and cold-responsive gene expression
    Joseph G Dubouzet
    Biological Resources Division, Japan International Research Center for Agricultural Sciences (JIRCAS, 1-1 Ohwashi, Tsukuba, Ibaraki 305-8686, Japan
    Plant J 33:751-63. 2003
    ..OsDREB1A is potentially useful for producing transgenic monocots that are tolerant to drought, high-salt, and/or cold stresses...
  11. ncbi Constitutive expression of the CIRCADIAN CLOCK ASSOCIATED 1 (CCA1) gene disrupts circadian rhythms and suppresses its own expression
    Z Y Wang
    Department of Molecular, Cell, and Developmental Biology, UCLA, Los Angeles, California 90095 1606, USA
    Cell 93:1207-17. 1998
    ..Furthermore, the expression of both endogenous CCA1 and the related LHY gene was suppressed. Our results suggest that CCA1 is a part of a feedback loop that is closely associated with the circadian clock in Arabidopsis...
  12. ncbi The Arabidopsis abscisic acid response gene ABI5 encodes a basic leucine zipper transcription factor
    R R Finkelstein
    Department of Molecular, Cellular and Developmental Biology, University of California Santa Barbara, Santa Barbara, California 93106, USA
    Plant Cell 12:599-609. 2000
    ..Comparison of seed and ABA-inducible vegetative gene expression in wild-type and abi5-1 plants indicates that ABI5 regulates a subset of late embryogenesis-abundant genes during both developmental stages...
  13. ncbi Arabidopsis AtMYC2 (bHLH) and AtMYB2 (MYB) function as transcriptional activators in abscisic acid signaling
    Hiroshi Abe
    Biological Resources Division, Japan International Research Center for Agricultural Sciences, 1 1 Ohwashi, Tsukuba, Ibaraki 305 8686, Japan
    Plant Cell 15:63-78. 2003
    ..These results indicate that both AtMYC2 and AtMYB2 proteins function as transcriptional activators in ABA-inducible gene expression under drought stress in plants...
  14. ncbi The oxylipin signal jasmonic acid is activated by an enzyme that conjugates it to isoleucine in Arabidopsis
    Paul E Staswick
    Department of Agronomy and Horticulture, University of Nebraska, Lincoln, Nebraska 68583, USA
    Plant Cell 16:2117-27. 2004
    ..Plant hormone activation by conjugation to amino acids and the enzymes involved in their formation were previously unknown...
  15. ncbi Arabidopsis LEAFY COTYLEDON1 is sufficient to induce embryo development in vegetative cells
    T Lotan
    Division of Biological Sciences, University of California, Davis 95616, USA
    Cell 93:1195-205. 1998
    ..Our results suggest that LEC1 is an important regulator of embryo development that activates the transcription of genes required for both embryo morphogenesis and cellular differentiation...
  16. ncbi Hierarchical action and inhibition of plant Dicer-like proteins in antiviral defense
    Angelique Deleris
    Institut de Biologie Moleculaire des Plantes, CNRS Unité Propre de Recherche UPR 2357, 12, rue du Général Zimmer, 67084 Strasbourg Cedex, France
    Science 313:68-71. 2006
    ..These findings define a molecular framework for studying antiviral silencing and defense in plants...
  17. ncbi Distinct roles of CONSTANS target genes in reproductive development of Arabidopsis
    A Samach
    John Innes Centre, Norwich NR4 7UH, UK
    Science 288:1613-6. 2000
    ..The SOC1 and FT genes are also regulated by a second flowering-time pathway that acts independently of CO. Thus, early target genes of CO define common components of distinct flowering-time pathways...
  18. ncbi Construct design for efficient, effective and high-throughput gene silencing in plants
    S V Wesley
    CSIRO Plant Industry, PO Box 1600 Canberra ACT 2601, Australia
    Plant J 27:581-90. 2001
    ..This system may facilitate the large-scale determination and discovery of plant gene functions in the same way as RNAi is being used to examine gene function in Caenorhabditis elegans...
  19. ncbi Arabidopsis ARGONAUTE1 is an RNA Slicer that selectively recruits microRNAs and short interfering RNAs
    N Baumberger
    Sainsbury Laboratory, Norwich Research Park, Norwich NR4 7UH, United Kingdom
    Proc Natl Acad Sci U S A 102:11928-33. 2005
    ..Based on sequence similarity, we predict that other Arabidopsis AGOs might have a similar catalytic activity but recruit different subsets of siRNAs or miRNAs...
  20. ncbi The Arabidopsis CORONATINE INSENSITIVE1 protein is a jasmonate receptor
    Jianbin Yan
    Department of Biological Sciences, Tsinghua University, Beijing 100084, China
    Plant Cell 21:2220-36. 2009
    ..Taken together, these results demonstrate that COI1 directly binds to JA-Ile and COR and serves as a receptor for jasmonate...
  21. ncbi The R1 gene for potato resistance to late blight (Phytophthora infestans) belongs to the leucine zipper/NBS/LRR class of plant resistance genes
    Agim Ballvora
    , , , Germany
    Plant J 30:361-71. 2002
    ..R1 is located within a hot spot for pathogen resistance on potato chromosome V. In comparison to the susceptibility allele, the resistance allele at the R1 locus represents a large insertion of a functional R gene...
  22. ncbi A Pseudomonas syringae type III effector suppresses cell wall-based extracellular defense in susceptible Arabidopsis plants
    Paula Hauck
    Department of Energy Plant Research Laboratory and Department of Plant Biology, Michigan State University, East Lansing, MI 48824, USA
    Proc Natl Acad Sci U S A 100:8577-82. 2003
    ..AvrPto is therefore one of the long postulated suppressors of an salicylic acid-independent, cell wall-based defense that is aimed at hrp mutant bacteria...
  23. ncbi Antisense LOX expression increases herbivore performance by decreasing defense responses and inhibiting growth-related transcriptional reorganization in Nicotiana attenuata
    Rayko Halitschke
    Department of Molecular Ecology, Max Planck Institute for Chemical Ecology, , D-07745 Jena, Germany
    Plant J 36:794-807. 2003
    ..By genetically manipulating signaling in a plant with a well-characterized ecology, we demonstrate that the complex phenotypic changes that mediate herbivore resistance are controlled by a specific part of the oxylipin cascade...
  24. ncbi The putative plasma membrane Na(+)/H(+) antiporter SOS1 controls long-distance Na(+) transport in plants
    Huazhong Shi
    Department of Plant Sciences, University of Arizona, Tucson, Arizona 85721, USA
    Plant Cell 14:465-77. 2002
    ..We present a model in which SOS1 functions in retrieving Na(+) from the xylem stream under severe salt stress, whereas under mild salt stress it may function in loading Na(+) into the xylem...
  25. ncbi Functional analysis of a NAC-type transcription factor OsNAC6 involved in abiotic and biotic stress-responsive gene expression in rice
    Kazuo Nakashima
    Biological Resources Division, Japan International Research Center for Agricultural Sciences, Tsukuba, Ibaraki 305 8686, Japan
    Plant J 51:617-30. 2007
    ..We conclude that OsNAC6 may serve as a useful biotechnological tool for the improvement of stress tolerance in various kinds of plants...
  26. ncbi An ancient R gene from the wild potato species Solanum bulbocastanum confers broad-spectrum resistance to Phytophthora infestans in cultivated potato and tomato
    Edwin van der Vossen
    Plant Research International BV, PO Box 16, 6700 AA Wageningen, The Netherlands
    Plant J 36:867-82. 2003
    ..These data suggest that Rpi-blb1 is relatively old and may be subject to balancing selection...
  27. ncbi OsWRKY13 mediates rice disease resistance by regulating defense-related genes in salicylate- and jasmonate-dependent signaling
    Deyun Qiu
    National Key Laboratory of Crop Genetic Improvement, National Center for Plant Gene Research, Huazhong Agricultural University, Wuhan 430070, China
    Mol Plant Microbe Interact 20:492-9. 2007
    ..Furthermore, OsWRKY13 will provide a transgenic tool for engineering wider-spectrum and whole-growth-stage resistance rice in breeding programs...
  28. ncbi AtNAC2, a transcription factor downstream of ethylene and auxin signaling pathways, is involved in salt stress response and lateral root development
    Xin Jian He
    National Key Lab of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
    Plant J 44:903-16. 2005
    ..AtNAC2 also promoted or inhibited downstream gene expressions. These results indicate that AtNAC2 may be a transcription factor incorporating the environmental and endogenous stimuli into the process of plant lateral root development...
  29. ncbi MicroRNA directs mRNA cleavage of the transcription factor NAC1 to downregulate auxin signals for arabidopsis lateral root development
    Hui Shan Guo
    Laboratory of Molecular Cell Biology, Temasek Life Science Laboratory, National University of Singapore, 117604 Singapore
    Plant Cell 17:1376-86. 2005
    ..Moreover, the cleavage-resistant form of NAC1 mRNA was unaffected by auxin treatment. Our results indicate that auxin induction of miR164 provides a homeostatic mechanism to clear NAC1 mRNA to downregulate auxin signals...
  30. ncbi CONSTANS acts in the phloem to regulate a systemic signal that induces photoperiodic flowering of Arabidopsis
    Hailong An
    Max Planck Institute for Plant Breeding, Carl von Linne Weg 10, D-50829 Cologne, Germany
    Development 131:3615-26. 2004
    ....
  31. ncbi Trifurcate feed-forward regulation of age-dependent cell death involving miR164 in Arabidopsis
    Jin Hee Kim
    Division of Molecular Life Sciences, Pohang University of Science and Technology, Hyoja Dong, Pohang, Kyungbuk, 790 784, Republic of Korea
    Science 323:1053-7. 2009
    ..The trifurcate feed-forward pathway involving ORE1, miR164, and EIN2 provides a highly robust regulation to ensure that aging induces cell death in Arabidopsis leaves...
  32. ncbi The transcription factor FLC confers a flowering response to vernalization by repressing meristem competence and systemic signaling in Arabidopsis
    Iain Searle
    Max Planck Institute for Plant Breeding, D 50829 Cologne, Germany
    Genes Dev 20:898-912. 2006
    ..Thus vernalization relieves transcriptional repression of key regulatory genes in both the leaf and meristem, allowing production of systemic signals in the leaves and conferring competence on the meristem to respond to these signals...
  33. ncbi Gene RB cloned from Solanum bulbocastanum confers broad spectrum resistance to potato late blight
    Junqi Song
    Department of Horticulture, Biotechnology Center, University of Wisconsin, Madison, WI 53706, USA
    Proc Natl Acad Sci U S A 100:9128-33. 2003
    ..Our results also demonstrate that LR-PCR is a valuable approach to isolate genes that cannot be maintained in the bacterial artificial chromosome system...
  34. ncbi Systemic Agrobacterium tumefaciens-mediated transfection of viral replicons for efficient transient expression in plants
    Sylvestre Marillonnet
    Icon Genetics, Biozentrum Halle, Weinbergweg 22, D-06120 Halle (Saale, Germany
    Nat Biotechnol 23:718-23. 2005
    ..tumefaciens, the high expression yield obtained with viral vectors, and the post-translational capabilities of a plant), does not require genetic modification of plants and is faster than other existing methods...
  35. ncbi Quantitative profiling of polar glycerolipid species from organs of wild-type Arabidopsis and a phospholipase Dalpha1 knockout mutant
    Shivakumar Pattada Devaiah
    Department of Biochemistry, Kansas State University, Manhattan, KS 66506, USA
    Phytochemistry 67:1907-24. 2006
    ....
  36. ncbi ABI5 acts downstream of ABI3 to execute an ABA-dependent growth arrest during germination
    Luis Lopez-Molina
    Laboratory of Plant Molecular Biology, Laboratory for Mass Spectrometry and Gaseous Ion Chemistry, The Rockefeller University, 1230 York Avenue, New York, NY 10021-6399, USA
    Plant J 32:317-28. 2002
    ..Although ABI5 is consistently located in the nucleus, chromosomal immunoprecipitation (ChIP) experiments revealed that ABA increases ABI5 occupancy on the AtEm6 promoter...
  37. ncbi Dependence of stem cell fate in Arabidopsis on a feedback loop regulated by CLV3 activity
    U Brand
    Institut fur Entwicklungsbiologie, Universitat zu Koln, Gyrhofstrabetae 17, D 50923 Köln, Germany
    Science 289:617-9. 2000
    ..We also demonstrate that the CLV pathway acts by repressing the activity of the transcription factor WUSCHEL, an element of the positive, stem cell-promoting pathway...
  38. ncbi Induction of protein secretory pathway is required for systemic acquired resistance
    Dong Wang
    Developmental, Cell and Molecular Biology Group, Department of Biology, Post Office Box 91000, Duke University, Durham, NC 27708, USA
    Science 308:1036-40. 2005
    ..Activation of this cis-element is controlled by a transcription factor that is translocated into the nucleus upon SAR induction...
  39. ncbi Modular cloning in plant cells
    Mansour Karimi
    Department of Plant Systems Biology, Flanders Interuniversity Institute for Biotechnology (VIB, Ghent University, Technologiepark 927, B-9052 Gent, Belgium
    Trends Plant Sci 10:103-5. 2005
    ..In addition, novel binary T-DNA vectors are now available to assemble multiple DNA fragments simultaneously, which greatly facilitate plant cell and protein engineering...
  40. ncbi Red colouration in apple fruit is due to the activity of the MYB transcription factor, MdMYB10
    Richard V Espley
    HortResearch, Mt Albert Research Centre, Private Bag 92169, Auckland, New Zealand
    Plant J 49:414-27. 2007
    ..Characterization of MdMYB10 has implications for the development of new varieties through classical breeding or a biotechnological approach...
  41. ncbi Signal transduction in maize and Arabidopsis mesophyll protoplasts
    J Sheen
    Department of Molecular Biology, Massachusetts General Hospital, Wellman 11, 50 Blossom Street, Boston, Massachusetts 02114, USA
    Plant Physiol 127:1466-75. 2001
    ....
  42. ncbi Functional analysis of an Arabidopsis transcription factor, DREB2A, involved in drought-responsive gene expression
    Yoh Sakuma
    Biological Resources Division, Japan International Research Center for Agricultural Sciences, Tsukuba, Ibaraki 305 8686, Japan
    Plant Cell 18:1292-309. 2006
    ..The region between DREB2A residues 136 and 165 plays a role in the stability of this protein in the nucleus, which is important for protein activation...
  43. ncbi pGreen: a versatile and flexible binary Ti vector for Agrobacterium-mediated plant transformation
    R P Hellens
    John Innes Centre, Norwich Research Park, Colney, UK
    Plant Mol Biol 42:819-32. 2000
    ..Information on the pGreen plasmid system is supplemented by an Internet site (http://www.pgreen.ac.uk) through which comprehensive information, protocols, order forms and lists of different pGreen marker gene permutations can be found...
  44. ncbi P1/HC-Pro, a viral suppressor of RNA silencing, interferes with Arabidopsis development and miRNA unction
    Kristin D Kasschau
    Center for Gene Research and Biotechnology and Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR 97331, USA
    Dev Cell 4:205-17. 2003
    ..The basis for TuMV- and other virus-induced disease in plants may be explained, at least partly, by interference with miRNA-controlled developmental pathways that share components with the antiviral RNA-silencing pathway...
  45. ncbi An Arabidopsis thaliana T-DNA mutagenized population (GABI-Kat) for flanking sequence tag-based reverse genetics
    Mario G Rosso
    GABI-Kat, Max Planck Institute for Plant Breeding Research, , , Germany
    Plant Mol Biol 53:247-59. 2003
    ..Finally, the functionality of the GABI-Kat population was demonstrated by exemplary confirmation of several new transparent testa alleles, as well as a number of other mutants, which were identified on the basis of the FST data...
  46. ncbi FLOWERING LOCUS T protein may act as the long-distance florigenic signal in the cucurbits
    Ming Kuem Lin
    Section of Plant Biology, College of Biological Sciences, University of California, Davis, California 95616, USA
    Plant Cell 19:1488-506. 2007
    ..maxima FT, but not FT mRNA, crossed the graft union in the phloem translocation stream. Collectively, these studies are consistent with FT functioning as a component of the florigenic signaling system in the cucurbits...
  47. ncbi MYB58 and MYB63 are transcriptional activators of the lignin biosynthetic pathway during secondary cell wall formation in Arabidopsis
    Jianli Zhou
    Department of Plant Biology, University of Georgia, Athens, Georgia 30602, USA
    Plant Cell 21:248-66. 2009
    ..Together, our results indicate that MYB58 and MYB63 are specific transcriptional activators of lignin biosynthesis in the SND1-mediated transcriptional network regulating secondary wall formation...
  48. ncbi Ligand-induced endocytosis of the pattern recognition receptor FLS2 in Arabidopsis
    Silke Robatzek
    Zurich Basel Plant Science Center, Botanical Institute, University of Basel, 4056 Basel, Switzerland
    Genes Dev 20:537-42. 2006
    ..We propose that plant cells regulate pathogen-associated molecular pattern (PAMP)-mediated PRR activities by subcellular compartmentalization...
  49. ncbi WRINKLED1 specifies the regulatory action of LEAFY COTYLEDON2 towards fatty acid metabolism during seed maturation in Arabidopsis
    Sébastien Baud
    Laboratoire de Biologie des Semences, IJPB, UMR 204 INRA AgroParis Tech, F 78026 Versailles, France
    Plant J 50:825-38. 2007
    ..thaliana seeds. In addition, they exemplify how metabolic and developmental processes affecting the developing embryo can be coordinated at the molecular level...
  50. ncbi In planta engineering of viral RNA replicons: efficient assembly by recombination of DNA modules delivered by Agrobacterium
    Sylvestre Marillonnet
    Icon Genetics, Biozentrum Halle, Weinbergweg 22, D-06120 Halle/Saale, Germany
    Proc Natl Acad Sci U S A 101:6852-7. 2004
    ....
  51. ncbi Genome-wide analysis of Arabidopsis thaliana DNA methylation uncovers an interdependence between methylation and transcription
    Daniel Zilberman
    Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, Seattle, Washington 98109, USA
    Nat Genet 39:61-9. 2007
    ..Our results indicate that genic transcription and DNA methylation are closely interwoven processes...
  52. ncbi A protein phosphatase 2C involved in ABA signal transduction in Arabidopsis thaliana
    K Meyer
    Institute of Plant Sciences, Swiss Federal Institute of Technology, Zurich
    Science 264:1452-5. 1994
    ..Thus, the control of the phosphorylation state of cell signaling components by the ABI1 product could mediate pleiotropic hormone responses...
  53. ncbi Dof domain proteins: plant-specific transcription factors associated with diverse phenomena unique to plants
    Shuichi Yanagisawa
    Research Institute for Bioresources, Okayama University, Chuo 2 20 1, Kurashiki, 710 0046 Japan
    Plant Cell Physiol 45:386-91. 2004
    ..Here I summarize our current knowledge about Dof domain proteins...
  54. ncbi Specific effects of microRNAs on the plant transcriptome
    Rebecca Schwab
    Department of Molecular Biology, Max Planck Institute for Developmental Biology, , Germany
    Dev Cell 8:517-27. 2005
    ..This finding unifies the view of plant miRNA action...
  55. ncbi Regulation of flowering time and floral organ identity by a MicroRNA and its APETALA2-like target genes
    Milo J Aukerman
    E I duPont de Nemours and Company, Agriculture and Nutrition, Newark, Delaware 19711, USA
    Plant Cell 15:2730-41. 2003
    ..Gain-of-function and loss-of-function analyses indicate that two of the AP2-like target genes normally act as floral repressors, supporting the notion that miR172 regulates flowering time by downregulating AP2-like target genes...
  56. ncbi Down-regulation of the maize and Arabidopsis thaliana caffeic acid O-methyl-transferase genes by two new maize R2R3-MYB transcription factors
    Silvia Fornalé
    Departament de Genetica Molecular, Laboratori de Genètica Molecular Vegetal, CSIC IRTA, Jordi Girona 18 26, 08034, Barcelona, Spain
    Plant Mol Biol 62:809-23. 2006
    ..thaliana and the maize COMT genes. Furthermore, the over-expression of ZmMYB31 and ZmMYB42 also affect the expression of other genes of the lignin pathway and produces a decrease in lignin content of the transgenic plants...
  57. ncbi LEAFY COTYLEDON1 controls seed storage protein genes through its regulation of FUSCA3 and ABSCISIC ACID INSENSITIVE3
    Yasuaki Kagaya
    Life Science Research Center, Mie University, Tsu, 514 8507 Japan
    Plant Cell Physiol 46:399-406. 2005
    ..From these data, we suggest that LEC1 controls the expression of the SSP genes in a hierarchical manner, which involves ABI3 and FUS3...
  58. ncbi Two transcription factors, DREB1 and DREB2, with an EREBP/AP2 DNA binding domain separate two cellular signal transduction pathways in drought- and low-temperature-responsive gene expression, respectively, in Arabidopsis
    Q Liu
    Biological Resources Division, Japan International Research Center for Agricultural Sciences JIRCAS, Ministry of Agriculture, Forestry, and Fisheries, 2 1 Ohwashi, Tsukuba, Ibaraki 305 8686, Japan
    Plant Cell 10:1391-406. 1998
    ..These results indicate that two independent families of DREB proteins, DREB1 and DREB2, function as trans-acting factors in two separate signal transduction pathways under low-temperature and dehydration conditions, respectively...
  59. ncbi SIZ1-mediated sumoylation of ICE1 controls CBF3/DREB1A expression and freezing tolerance in Arabidopsis
    Kenji Miura
    Center for Plant Environmental Stress Physiology, Purdue University, West Lafayette, Indiana 47907 2010, USA
    Plant Cell 19:1403-14. 2007
    ..SIZ1-dependent sumoylation of ICE1 may activate and/or stabilize the protein, facilitating expression of CBF3/DREB1A and repression of MYB15, leading to low temperature tolerance...
  60. ncbi Photoreceptor regulation of CONSTANS protein in photoperiodic flowering
    Federico Valverde
    Max Planck Institute for Plant Breeding, Carl-von-Linne Weg 10, D-50829 Cologne, Germany
    Science 303:1003-6. 2004
    ..This layer of regulation refines the circadian rhythm in CO messenger RNA and is central to the mechanism by which day length controls flowering...
  61. ncbi The biosynthesis and nutritional uses of carotenoids
    Paul D Fraser
    School of Biological Sciences, Royal Holloway, University of London, Egham, Surrey TW20 0EX, UK
    Prog Lipid Res 43:228-65. 2004
    ....
  62. ncbi Sugar and ABA responsiveness of a minimal RBCS light-responsive unit is mediated by direct binding of ABI4
    Gustavo Javier Acevedo-Hernández
    Departamento de Ingeniería Genética de Plantas, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional Unidad Irapuato, Apartado Postal 629, Irapuato, Guanajuato 36500, Mexico
    Plant J 43:506-19. 2005
    ..This phylogenetically conserved promoter feature probably reflects a common regulatory mechanism and identifies a point of convergence between light- and sugar-signaling pathways...
  63. ncbi Molecular farming in plants: host systems and expression technology
    Richard M Twyman
    Department of Biology, University of York, Heslington, York YO10 5DD, UK
    Trends Biotechnol 21:570-8. 2003
  64. ncbi Characterization of transcription factor gene SNAC2 conferring cold and salt tolerance in rice
    Honghong Hu
    National Center of Plant Gene Research Wuhan, National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, China
    Plant Mol Biol 67:169-81. 2008
    ..These data suggest SNAC2 is a novel stress responsive NAC transcription factor that possesses potential utility in improving stress tolerance of rice...
  65. ncbi Problem formulation in the environmental risk assessment for genetically modified plants
    Jeffrey D Wolt
    Biosafety Institute for Genetically Modified Agricultural Products, Iowa State University, Ames, IA, USA
    Transgenic Res 19:425-36. 2010
    ..This paper is the product of an international expert group convened by the International Life Sciences Institute (ILSI) Research Foundation...
  66. ncbi Dual function of an Arabidopsis transcription factor DREB2A in water-stress-responsive and heat-stress-responsive gene expression
    Yoh Sakuma
    Graduate School of Agricultural and Life Sciences, University of Tokyo, Tokyo 113 8657, Japan
    Proc Natl Acad Sci U S A 103:18822-7. 2006
    ..Thermotolerance was significantly increased in plants overexpressing DREB2A CA and decreased in DREB2A knockout plants. Collectively, these results indicate that DREB2A functions in both water and HS-stress responses...
  67. ncbi Altered circadian rhythms regulate growth vigour in hybrids and allopolyploids
    Zhongfu Ni
    Section of Molecular Cell and Developmental Biology, The University of Texas at Austin, One University Station, A 4800, Austin, Texas 78712, USA
    Nature 457:327-31. 2009
    ..The causal effects of CCA1 on output traits suggest that hybrids and allopolyploids gain advantages from the control of circadian-mediated physiological and metabolic pathways, leading to growth vigour and increased biomass...
  68. ncbi Infection and coaccumulation of tobacco mosaic virus proteins alter microRNA levels, correlating with symptom and plant development
    A A Bazzini
    Instituto de Biotecnologia, Centro de Investigación en Ciencias Veterinarias y Agronómicas, Instituto Nacional de Tecnología Agropecuaria Castelar, Las Cabañas y Los Reseros, B1712WAA Buenos Aires, Argentina
    Proc Natl Acad Sci U S A 104:12157-62. 2007
    ..This work presents evidence that virus infection and viral proteins influence miRNA balance without affecting posttranscriptional gene silencing and contributes to the hypothesis that viruses exploit miRNA pathways during pathogenesis...
  69. ncbi Involvement of the MADS-box gene ZMM4 in floral induction and inflorescence development in maize
    Olga N Danilevskaya
    Pioneer Hi Bred International, Inc, A DuPont Company, Johnston, Iowa 50131, USA
    Plant Physiol 147:2054-69. 2008
    ..Our results suggest ZMM4 may play roles in both floral induction and inflorescence development...
  70. ncbi A phenylalanine in DGAT is a key determinant of oil content and composition in maize
    Peizhong Zheng
    Pioneer Hi Bred International Inc, A DuPont Company, 7300 NW 62nd Avenue, PO Box 1004, Johnston, Iowa 50131, USA
    Nat Genet 40:367-72. 2008
    ..This work provides insights into the molecular basis of natural variation of oil and oleic-acid contents in plants and highlights DGAT as a promising target for increasing oil and oleic-acid contents in other crops...
  71. ncbi Tissue-dependent enhancement of transgene expression by introns of replacement histone H3 genes of Arabidopsis
    N Chaubet-Gigot
    , CNRS, Universit Louis Pasteur, Strasbourg, France
    Plant Mol Biol 45:17-30. 2001
    ..Such factors appear most abundant in roots...
  72. ncbi The spen family protein FPA controls alternative cleavage and polyadenylation of RNA
    Csaba Hornyik
    Genetics, SCRI, Invergowrie, Dundee, DD2 5DA, Scotland, UK
    Dev Cell 18:203-13. 2010
    ..Transcript readthrough accounts for the absence of changes in DNA methylation and siRNA abundance at AtSN1 in fpa mutants, and this may explain other examples of epigenetic transitions not associated with chromatin modification...
  73. ncbi High-throughput fluorescent tagging of full-length Arabidopsis gene products in planta
    Guo-Wei Tian
    Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, New York 11794-5215, USA
    Plant Physiol 135:25-38. 2004
    ....
  74. ncbi Identification of a gene associated with Bt resistance in Heliothis virescens
    L J Gahan
    Department of Biological Sciences, Clemson University, Clemson, SC 29634, USA
    Science 293:857-60. 2001
    ..Our findings enable efficient DNA-based screening for resistant heterozygotes by directly detecting the recessive allele...
  75. ncbi Nicotiana benthamiana: its history and future as a model for plant-pathogen interactions
    Michael M Goodin
    Department of Plant Pathology, University of Kentucky, Lexington 40546, USA
    Mol Plant Microbe Interact 21:1015-26. 2008
    ..Finally, we propose that N. benthamiana is well situated to become a premier plant cell biology model, particularly for the virology community, who as a group were the first to recognize the potential of this unique Australian native...
  76. ncbi The magnesium-chelatase H subunit binds abscisic acid and functions in abscisic acid signaling: new evidence in Arabidopsis
    Fu Qing Wu
    State Key Laboratory of Plant Physiology and Biochemistry, China Agricultural University, Beijing 100094, China
    Plant Physiol 150:1940-54. 2009
    ....
  77. ncbi Xa26, a gene conferring resistance to Xanthomonas oryzae pv. oryzae in rice, encodes an LRR receptor kinase-like protein
    Xinli Sun
    National Key Laboratory of Crop Genetic Improvement, National Center of Crop Molecular Breeding, Huazhong Agricultural University, Wuhan 430070, China
    Plant J 37:517-27. 2004
    ..These results suggest that the resistance conferred by Xa26 is influenced by the genetic background...
  78. ncbi A plasmodesmata-associated beta-1,3-glucanase in Arabidopsis
    Amit Levy
    Department of Plant Sciences, George S Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel
    Plant J 49:669-82. 2007
    ..Taken together, we suggest that AtBG_ppap is a Pd-associated membrane protein involved in plasmodesmal callose degradation, and functions in the gating of Pd...
  79. ncbi Molecular characterization of the Arabidopsis 9-cis epoxycarotenoid dioxygenase gene family
    Bao Cai Tan
    Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, FL 32611, USA
    Plant J 35:44-56. 2003
    ..In addition, differential membrane-binding capacity of AtNCEDs is a potential means of post-translational regulation of NCED activity...
  80. ncbi Analysis of interactions among the CLAVATA3 receptors reveals a direct interaction between CLAVATA2 and CORYNE in Arabidopsis
    Yingfang Zhu
    Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100049, China
    Plant J 61:223-33. 2010
    ..Taken together, our results add direct evidence to the newly proposed two-parallel receptor pathways model and therefore provide new insights into the CLV3 signaling pathway...
  81. ncbi Down-regulating alpha-galactosidase enhances freezing tolerance in transgenic petunia
    Joyce C Pennycooke
    Department of Horticulture and Landscape Architecture, Colorado State University, Fort Collins, CO 80523, USA
    Plant Physiol 133:901-9. 2003
    ..These results suggest that engineering raffinose metabolism by transformation with alpha-Gal provides an additional method for improving the freezing tolerance of plants...
  82. ncbi Plant-based production of biopharmaceuticals
    Rainer Fischer
    Institute for Molecular Biotechnology, Biology VII, RWTH Aachen, Worringerweg 1, 52074 Aachen, Germany
    Curr Opin Plant Biol 7:152-8. 2004
    ..There has also been significant success in tackling some of the limitations of plant bioreactors, such as low yields and inconsistent product quality, that have limited the approval of plant-derived pharmaceuticals...
  83. ncbi Geminiviruses and RNA silencing
    Ramachandran Vanitharani
    International Laboratory for Tropical Agricultural Biotechnology, Donald Danforth Plant Science Center, 975 North Warson Road, St. Louis, MO 63132, USA
    Trends Plant Sci 10:144-51. 2005
    ....
  84. ncbi A PINOID-dependent binary switch in apical-basal PIN polar targeting directs auxin efflux
    Jiri Friml
    Developmental Genetics, Center for Molecular Biology of Plants, University Tübingen, Auf der Morgenstelle 3, D 72076 Tubingen, Germany
    Science 306:862-5. 2004
    ..Our results show that a PINOID-dependent binary switch controls PIN polarity and mediates changes in auxin flow to create local gradients for patterning processes...
  85. ncbi Molecular basis of N-acetylglucosaminyltransferase I deficiency in Arabidopsis thaliana plants lacking complex N-glycans
    Richard Strasser
    Department für Angewandte Pflanzenwissenschaften und Pflanzenbiotechnologie, Institut für Angewandte Genetik und Zellbiologie, Universitat fur Bodenkultur Wien, Muthgasse 18, A 1190 Wien, Austria
    Biochem J 387:385-91. 2005
    ..These results indicate that the complete deficiency of GnTI activity in cgl plants is mainly due to the additional N-glycan, which appears to interfere with the proper folding of the enzyme...
  86. ncbi Cell-to-cell movement of potato potexvirus X is dependent on suppression of RNA silencing
    Elizabeth H Bayne
    The Sainsbury Laboratory, John Innes Centre, Colney Lane, Norwich NR4 7UH, UK
    Plant J 44:471-82. 2005
    ..It is possible, based on analyses of short interfering RNA accumulation, that P25 suppresses silencing by interfering with either assembly or function of the effector complexes of RNA silencing...
  87. ncbi Early infection of scutellum tissue with Agrobacterium allows high-speed transformation of rice
    Seiichi Toki
    Plant Genetic Engineering Research Unit, National Institute of Agrobiological Sciences, 2 1 2 Kannondai, Tsukuba, Ibaraki 305 8602, Japan
    Plant J 47:969-76. 2006
    ..Our new system should reduce the somaclonal variation accompanying prolonged culture of rice cells in the dedifferentiated state and facilitate the molecular breeding of rice...
  88. ncbi Cellular and subcellular localization of phototropin 1
    Koji Sakamoto
    Department of Plant Biology, Carnegie Institution of Washington, 260 Panama Street, Stanford, CA 94305, USA
    Plant Cell 14:1723-35. 2002
    ..Possible relationships between observed phot1 distribution and the various physiological responses activated by blue light are discussed...
  89. ncbi RNA silencing and the mobile silencing signal
    Sizolwenkosi Mlotshwa
    Department of Biological Sciences, University of South Carolina, Columbia, SC 29208, USA
    Plant Cell 14:S289-301. 2002
  90. ncbi Identification and characterization of the Arabidopsis PHO1 gene involved in phosphate loading to the xylem
    Dirk Hamburger
    , , , CH-1015 Lausanne, Switzerland
    Plant Cell 14:889-902. 2002
    ..The Arabidopsis genome contains 10 additional genes showing homology with PHO1. Thus, PHO1 defines a novel class of proteins involved in ion transport in plants...
  91. ncbi Production of a monoclonal antibody in plants with a humanized N-glycosylation pattern
    Matthias Schähs
    Institute of Applied Genetics and Cell Biology, BOKU Wien, Austria
    Plant Biotechnol J 5:657-63. 2007
    ..Our results demonstrate the feasibility of a knockout strategy for N-glycan engineering of plants towards mammalian-like structures, thus providing a significant improvement in the use of plants as an expression platform...
  92. ncbi Agrobacterium rhizogenes-transformed roots of Medicago truncatula for the study of nitrogen-fixing and endomycorrhizal symbiotic associations
    A Boisson-Dernier
    , INRA-CNRS UMR215, Castanet-Tolosan, France
    Mol Plant Microbe Interact 14:695-700. 2001
    ..Our results suggest that M. truncatula hairy roots represent a particularly attractive system with which to study endosymbiotic associations in transgenically modified roots...
  93. ncbi High-yield production of a human therapeutic protein in tobacco chloroplasts
    J M Staub
    Monsanto Company, 700 Chesterfield Village Parkway North, St Louis, MO 63198, USA
    Nat Biotechnol 18:333-8. 2000
    ..The results indicate that chloroplasts are a highly efficient vehicle for the potential production of pharmaceutical proteins in plants...
  94. ncbi PSEUDO-RESPONSE REGULATORS 9, 7, and 5 are transcriptional repressors in the Arabidopsis circadian clock
    Norihito Nakamichi
    RIKEN Plant Science Center, Plant Productivity Systems Research Group, Tsurumi, Yokohama 230 0045, Japan
    Plant Cell 22:594-605. 2010
    ..These results suggest that the repressor activities of PRR9, PRR7, and PRR5 on the CCA1 and LHY promoter regions constitute the molecular mechanism that accounts for the role of these proteins in the feedback loop of the circadian clock...
  95. ncbi A plant miRNA contributes to antibacterial resistance by repressing auxin signaling
    Lionel Navarro
    Sainsbury Laboratory, John Innes Centre, Colney Lane, Norwich NR4 7UH, UK
    Science 312:436-9. 2006
    ..Repression of auxin signaling restricts P. syringae growth, implicating auxin in disease susceptibility and miRNA-mediated suppression of auxin signaling in resistance...
  96. ncbi CLAVATA1 dominant-negative alleles reveal functional overlap between multiple receptor kinases that regulate meristem and organ development
    Anne Dievart
    Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor 48109 1048, USA
    Plant Cell 15:1198-211. 2003
    ..We propose the presence of multiple receptors that regulate meristem development in a functionally related manner whose interactions are driven by the extracellular domains and whose activation requires the kinase domain...
  97. ncbi Cytokinin-dependent photorespiration and the protection of photosynthesis during water deficit
    Rosa M Rivero
    Department Plant Sciences, University of California, Davis, California 95616, USA
    Plant Physiol 150:1530-40. 2009
    ..Moreover, the increase in transcripts indicated a cytokinin-induced elevation in photorespiration, suggesting the contribution of photorespiration in the protection of photosynthetic processes and its beneficial role during water stress...
  98. ncbi Geminivirus AL2 and L2 proteins interact with and inactivate SNF1 kinase
    Linhui Hao
    Department of Molecular Genetics, Plant Biotechnology Center, The Ohio State University, Columbus, Ohio 43210, USA
    Plant Cell 15:1034-48. 2003
    ..They also indicate that geminiviruses are able to modify host metabolism to their own advantage, and they provide a molecular link between metabolic status and inherent susceptibility to viral pathogens...
  99. ncbi Arabidopsis DREB2A-interacting proteins function as RING E3 ligases and negatively regulate plant drought stress-responsive gene expression
    Feng Qin
    Biological Resources Division, Japan International Research Center for Agricultural Sciences, Tsukuba, Ibaraki 305 8686, Japan
    Plant Cell 20:1693-707. 2008
    ..These results suggest that DRIP1 and DRIP2 act as novel negative regulators in drought-responsive gene expression by targeting DREB2A to 26S proteasome proteolysis...
  100. ncbi Functional analysis of Arabidopsis ethylene-responsive element binding protein conferring resistance to Bax and abiotic stress-induced plant cell death
    Taro Ogawa
    Institute of Molecular and Cellular Biosciences, The University of Tokyo, Tokyo 113 0032, Japan
    Plant Physiol 138:1436-45. 2005
    ..Up-regulation of defense genes was observed in transgenic Arabidopsis plants overexpressing AtEBP. Based on the analysis of mRNA accumulation in ethylene-related mutants, the position of AtEBP in signaling pathway is presented...
  101. ncbi Constitutive expression of Arabidopsis NPR1 confers enhanced resistance to the early instars of Spodoptera litura in transgenic tobacco
    Gargi Meur
    School of Life Sciences, University of Hyderabad, Hyderabad 500046, India
    Physiol Plant 133:765-75. 2008
    ..We show in this communication that heterologous expression of AtNPR1 provides enhanced resistance to early larval populations of the herbivore, Spodoptera in transgenic tobacco plants...

Research Grants90

  1. Expression of Human Therapeutic Proteins in Chloroplasts
    Henry Daniell; Fiscal Year: 2005
    ..Large scale and low cost production via transgenic chloroplasts should provide treatment to patients at an affordable cost and tobacco farmers alternate uses for this hazardous crop. ..
  2. Artemisinin Biosynthesis: Control of Transcription
    Pamela Weathers; Fiscal Year: 2007
    ..The proposed study is a first step in helping us understand how to control artemisinin's biosynthesis to ultimately increase the production of this drug, and others that are chemically similar, in low cost field-grown plants. ..
  3. Rhabdovirus phosphoproteins: RNA silencing and complex formation
    Michael Goodin; Fiscal Year: 2007
    ....
  4. Mammalian Lactoferrin Receptors: Structure and Function
    Bo Lonnerdal; Fiscal Year: 2007
    ..Overall, our understanding of the physiological significance of Lf and its receptor will be increased. ..
  5. Expression of Human Therapeutic Proteins in Transgenic Tobacco Chloroplasts
    Henry Daniell; Fiscal Year: 2006
    ..Successful completion of these studies would make transgenic chloroplasts ideal bioreactors for the production of safe and less expensive therapeutic proteins and open the door for in depth studies of membrane proteins. ..
  6. Expression of Human Therapeutic Proteins in Transgenic Tobacco Chloroplasts
    Henry Daniell; Fiscal Year: 2010
    ..Successful completion of these studies would make transgenic chloroplasts ideal bioreactors for the production of safe and less expensive therapeutic proteins and open the door for in depth studies of membrane proteins. ..
  7. Expression of Human Therapeutic Proteins in Transgenic Tobacco Chloroplasts
    Henry Daniell; Fiscal Year: 2009
    ..Successful completion of these studies would make transgenic chloroplasts ideal bioreactors for the production of safe and less expensive therapeutic proteins and open the door for in depth studies of membrane proteins. ..
  8. Drosophila Transgenic RNAi Resource Project
    Norbert Perrimon; Fiscal Year: 2009
    ....
  9. Functional Genomic analysis using RNAi screen in Drosophila
    Norbert Perrimon; Fiscal Year: 2010
    ....
  10. DOSAGE COMPENSATION IN DROSOPHILA
    James A Birchler; Fiscal Year: 2010
    ..The single active X chromosome in humans of both males and females must be up-regulated for viability. The proposed project will examine the type of mechanism responsible for this up-regulation. ..
  11. Development and Testing of a Novel Neuroprotective Drug
    David Schubert; Fiscal Year: 2006
    ..The results from these studies will determine the therapeutic potential of this novel compound. ..
  12. Network analysis of Signal Transduction
    Norbert Perrimon; Fiscal Year: 2010
    ..The success of this application will provide a benchmark for reconstructing signaling networks on a global scale. ..
  13. Mucosal Vaccines for Plague
    David Pascual; Fiscal Year: 2006
    ..pestis challenge. From these studies, we will produce efficacious vaccines to plague, as well as a supporting vaccine regimen, that will confer protective immunity in the mucosal and systemic immune compartments. ..
  14. Regulation of APP Degradation and ABeta Secretion
    David Schubert; Fiscal Year: 2007
    ..In addition, understanding how cells regulate the degradation of proteins is critical to the study of a wide range of diseases, particularly those in the nervous system that are characterized by the intracellular protein accumulation. ..
  15. MUCOSAL IMMUNITY ELICITED BY SALMONELLA VACCINE DELIVERY
    David Pascual; Fiscal Year: 2009
    ..Thus, from these studies we will learn the specific mechanisms induced by this anti-inflammatory vaccine. ..
  16. Mucosal Lymphocyte Homing in the Head and Neck
    David Pascual; Fiscal Year: 2007
    ..n. challenge with CT. From this work, we will learn how routes of immunizations can be used to effectively target vaccines to nonintestinal mucosal sites. ..
  17. Characterization of a virus-activated RNA-induced silencing complex(RISC)
    HERMAN SCHOLTHOF; Fiscal Year: 2007
    ..Second, since virus infections produce high amounts of small RNAs (i.e. siRNAs); they represent useful model systems towards improving new strategies for therapeutic administration of high dosages of siRNAs. ..
  18. MUCOSAL IMMUNITY ELICITED BY SALMONELLA VACCINE DELIVERY
    David Pascual; Fiscal Year: 2007
    ..Thus, from these studies we will learn the specific mechanisms induced by this anti-inflammatory vaccine. ..
  19. High-throughput screening assay development for axonopathic neurodegeration
    Norbert Perrimon; Fiscal Year: 2006
    ..The long term goal of this work is thus to understand better the causes of axonal destruction in neurodegenerative diseases, and to find drugs that can slow or prevent axonal degeneration from occurring. ..
  20. Host factors involved in bacterial pathogenesis
    Norbert Perrimon; Fiscal Year: 2007
    ..tuberculosis and its human host. ..
  21. Pan-Lipoxygenase Inhibitors for CNS Disease
    DAVID R SCHUBERT; Fiscal Year: 2010
    ..To push these drugs to the clinic and to identify more and perhaps better therapeutic targets, we need to understand exactly how these drugs function, and that is the goal of this proposal. ..
  22. STRUCTURE AND FUNCTION OF AN RNA POL II CTD PHOSPHATASE
    JAMES OMICHINSKI; Fiscal Year: 2002
    ..In addition, our studies will provide new insights into how disruptions in the control of transcriptional regulation may occur in many forms of human cancer and viral infections. ..
  23. PHYSIOLOGY OF NERVE, GLIAL, AND MUSCLE CELL LINES
    David Schubert; Fiscal Year: 1980
    ..The role of extracellular substrate attached material in cell-cell and cell-substratum adhesion will also be studied. ..
  24. PHYSIOLOGY OF NERVE, GLIAL AND MUSCLE CELL LINES
    David Schubert; Fiscal Year: 1992
    ..The extracellular molecules released by regenerating sciatic nerve in vivo will be studied with respect to their effects on Schwann cell migraton, adhesion, and differentiation...
  25. PHYSIOLOGY OF NERVE, GLIAL, AND MUSCLE CELL LINES
    David Schubert; Fiscal Year: 1999
    ..The experiments in this proposal should help define the role of protein growth factors in the developing nervous system. Hopefully, this information will lead to a treatment for some neurodegenerative diseases and CNS trauma. ..
  26. MEMBRANE FUNCTION IN MUTANTS OF CAENORHABDITIS ELEGANS
    John Browse; Fiscal Year: 2004
    ..Quantitative measures of low-temperature performance and thermotolerance of the range of desaturase mutants will be correlated with fatty acid composition and measurements of membrane fluidity. ..
  27. High-throughput screen:Listeria infection (RMI)
    Norbert Perrimon; Fiscal Year: 2004
    ....
  28. PHYSIOLOGY OF NERVE, GLIAL AND MUSCLE CELL LINES
    David Schubert; Fiscal Year: 2005
    ..This work is directly relevant to CNS trauma and stroke as well as to all chronic degenerative diseases where oxidative stress is a pathological hallmark. ..
  29. CAM Intervention for Rheumatoid Arthritis
    David Pascual; Fiscal Year: 2007
    ..This application is responsive to RFA-AT-06-005: "Mechanisms of Immune Modulation" by addressing the requested research areas in understanding CAMs' mechanisms of action upon the induction and expansion of T cell regulatory populations. ..
  30. DOSAGE COMPENSATION IN DROSOPHILA
    James Birchler; Fiscal Year: 2006
    ..The proposed project will contribute to an understanding of how chromatin modifications become established along the length of the chromosome and how they affect gene expression. ..
  31. Conference on Cell Polarity & Asymmetric Cell Divisions
    Norbert Perrimon; Fiscal Year: 2004
    ..The goal of the meeting is to bring together the leading experts that study the mechanism of cell polarization and cell asymmetry to discuss emerging concepts. ..
  32. Functional Genomic Screen using RNAi in Drosophila
    Norbert Perrimon; Fiscal Year: 2006
    ..Finally, to demonstrate the value of the approach, we describe a number of cell-based screens that will identify genes involved in cell survival, cytokinesis, cell shape changes and immunity. ..
  33. Characterization of the Insulin to Autophagy Pathway in Muscles
    Norbert Perrimon; Fiscal Year: 2010
    ....
  34. Drosophila Transgenic RNAi Resource Project
    Norbert Perrimon; Fiscal Year: 2010
    ....
  35. Functional Genomic analysis using RNAi screen in Drosophila
    Norbert Perrimon; Fiscal Year: 2009
    ....
  36. Host factors involved in bacterial pathogenesis
    Norbert Perrimon; Fiscal Year: 2009
    ..tuberculosis and its human host. ..
  37. ROLES OF HEPARAN SULFATE PROTEOGLYCANS IN SIGNALING
    Norbert Perrimon; Fiscal Year: 2003
    ..Because these pathways have also been implicated in various disease stages; e.g., oncogenesis, our studies may lead to novel ways to modulate specifically the activity of these signaling pathways. ..
  38. Viral Immunity in Drosophila
    Norbert Perrimon; Fiscal Year: 2007
    ..Because our insect virus system is sensitive to drugs that are used against human viruses, our studies may lead to the identification of novel antiviral compounds that may prove effective in mammalian cells. ..
  39. New collections of dsRNAs and overexpression miRNA constructs to supplement RNAi
    Norbert Perrimon; Fiscal Year: 2006
    ..The opportunity to add these unconventional but clearly important genes to the list of targets that can be screened at the DRSC will reinforce the center's role as a leader in the field of genomic screens. ..
  40. Network Topology in Cell Signaling
    Norbert Perrimon; Fiscal Year: 2007
    ..Altogether, these studies will greatly advance our knowledge of signaling networks and their organization during signal transduction. ..