saccharomyces cerevisiae

Summary

Summary: A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. The dried form is used as a dietary supplement.

Top Publications

  1. ncbi The genetic landscape of a cell
    Michael Costanzo
    Banting and Best Department of Medical Research, Terrence Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario M5S 3E1, Canada
    Science 327:425-31. 2010
  2. ncbi The BioGRID Interaction Database: 2008 update
    Bobby Joe Breitkreutz
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Canada
    Nucleic Acids Res 36:D637-40. 2008
  3. ncbi Crystal structure of the eukaryotic ribosome
    Adam Ben-Shem
    IGBMC Institut de Génétique et de Biologie Moléculaire et Cellulaire, 1 rue Laurent Fries, BP10142, Illkirch F 67400, France
    Science 330:1203-9. 2010
  4. ncbi The transcriptional landscape of the yeast genome defined by RNA sequencing
    Ugrappa Nagalakshmi
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520, USA
    Science 320:1344-9. 2008
  5. ncbi Capturing chromosome conformation
    Job Dekker
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    Science 295:1306-11. 2002
  6. ncbi Population genomics of domestic and wild yeasts
    Gianni Liti
    Institute of Genetics, Queen s Medical Centre, University of Nottingham, Nottingham NG7 2UH, UK
    Nature 458:337-41. 2009
  7. ncbi Genome-wide analysis in vivo of translation with nucleotide resolution using ribosome profiling
    Nicholas T Ingolia
    Department of Cellular and Molecular Pharmacology, Howard Hughes Medical Institute, University of California, San Francisco, and California Institute for Quantitative Biosciences, San Francisco, CA 94158, USA
    Science 324:218-23. 2009
  8. ncbi Additional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiae
    M S Longtine
    Department of Biology, University of North Carolina, Chapel Hill 27599 3280, USA
    Yeast 14:953-61. 1998
  9. ncbi The chemical genomic portrait of yeast: uncovering a phenotype for all genes
    Maureen E Hillenmeyer
    Stanford Genome Technology Center, Stanford University, Palo Alto, CA 94304, USA
    Science 320:362-5. 2008
  10. ncbi Saccharomyces Genome Database provides mutant phenotype data
    Stacia R Engel
    Department of Genetics, Stanford University, Stanford, CA, USA
    Nucleic Acids Res 38:D433-6. 2010

Research Grants

  1. Role of the OPI1 gene in controlling viability of Candida glabrata
    TODD REYNOLDS; Fiscal Year: 2007
  2. ENZYMATIC MECHANISMS OF GENETIC RECOMBINATION
    Richard D Kolodner; Fiscal Year: 2010
  3. INTEGRATION SPECIFICITY OF THE TY3 RETROTRANSPOSON
    Suzanne Sandmeyer; Fiscal Year: 1999
  4. HTLV-I Tax activates the anaphase promoting complex
    Chou Zen Giam; Fiscal Year: 2009
  5. MECHANISM OF PRE-MRNA SPLICING
    MAGDA KONARSKA; Fiscal Year: 2009
  6. Regulation of nitrogen catabolic gene expression in S cerevisiae
    TERRANCE COOPER; Fiscal Year: 2007
  7. Regulation of nitrogen catabolic gene expression in S cerevisiae
    Terrance G Cooper; Fiscal Year: 2010
  8. MECHANISM OF PRE-MRNA SPLICING
    MAGDA KONARSKA; Fiscal Year: 2010
  9. MANNOPROTEIN BIOSYNTHESIS IN SACCHAROMYCES CEREVISIAE
    Annette Herscovics; Fiscal Year: 2000
  10. MECHANISM OF SITE SPECIFIC DNA RECOMBINATION
    Makkuni Jayaram; Fiscal Year: 2001

Detail Information

Publications238 found, 100 shown here

  1. ncbi The genetic landscape of a cell
    Michael Costanzo
    Banting and Best Department of Medical Research, Terrence Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario M5S 3E1, Canada
    Science 327:425-31. 2010
    ..quantitative genetic interaction profiles for approximately 75% of all genes in the budding yeast, Saccharomyces cerevisiae. A network based on genetic interaction profiles reveals a functional map of the cell in which genes of ..
  2. ncbi The BioGRID Interaction Database: 2008 update
    Bobby Joe Breitkreutz
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Canada
    Nucleic Acids Res 36:D637-40. 2008
    ..complete set of interactions reported to date in the primary literature for both the budding yeast Saccharomyces cerevisiae and the fission yeast Schizosaccharomyces pombe...
  3. ncbi Crystal structure of the eukaryotic ribosome
    Adam Ben-Shem
    IGBMC Institut de Génétique et de Biologie Moléculaire et Cellulaire, 1 rue Laurent Fries, BP10142, Illkirch F 67400, France
    Science 330:1203-9. 2010
    ..We describe the conformational changes in both ribosomal subunits that are involved in ratcheting and their implications in coordination between the two associated subunits and in mRNA and tRNA translocation...
  4. ncbi The transcriptional landscape of the yeast genome defined by RNA sequencing
    Ugrappa Nagalakshmi
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520, USA
    Science 320:1344-9. 2008
    ..We also found unexpected 3'-end heterogeneity and the presence of many overlapping genes. These results indicate that the yeast transcriptome is more complex than previously appreciated...
  5. ncbi Capturing chromosome conformation
    Job Dekker
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    Science 295:1306-11. 2002
    ..Using the yeast Saccharomyces cerevisiae, we could confirm known qualitative features of chromosome organization within the nucleus and dynamic ..
  6. ncbi Population genomics of domestic and wild yeasts
    Gianni Liti
    Institute of Genetics, Queen s Medical Centre, University of Nottingham, Nottingham NG7 2UH, UK
    Nature 458:337-41. 2009
    Since the completion of the genome sequence of Saccharomyces cerevisiae in 1996 (refs 1, 2), there has been a large increase in complete genome sequences, accompanied by great advances in our understanding of genome evolution...
  7. ncbi Genome-wide analysis in vivo of translation with nucleotide resolution using ribosome profiling
    Nicholas T Ingolia
    Department of Cellular and Molecular Pharmacology, Howard Hughes Medical Institute, University of California, San Francisco, and California Institute for Quantitative Biosciences, San Francisco, CA 94158, USA
    Science 324:218-23. 2009
    ..Ribosome profiling is readily adaptable to other organisms, making high-precision investigation of protein translation experimentally accessible...
  8. ncbi Additional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiae
    M S Longtine
    Department of Biology, University of North Carolina, Chapel Hill 27599 3280, USA
    Yeast 14:953-61. 1998
    An important recent advance in the functional analysis of Saccharomyces cerevisiae genes is the development of the one-step PCR-mediated technique for deletion and modification of chromosomal genes...
  9. ncbi The chemical genomic portrait of yeast: uncovering a phenotype for all genes
    Maureen E Hillenmeyer
    Stanford Genome Technology Center, Stanford University, Palo Alto, CA 94304, USA
    Science 320:362-5. 2008
    ..We found that 97% of gene deletions exhibited a measurable growth phenotype, suggesting that nearly all genes are essential for optimal growth in at least one condition...
  10. ncbi Saccharomyces Genome Database provides mutant phenotype data
    Stacia R Engel
    Department of Genetics, Stanford University, Stanford, CA, USA
    Nucleic Acids Res 38:D433-6. 2010
    ..yeastgenome.org) is a scientific database for the molecular biology and genetics of the yeast Saccharomyces cerevisiae, which is commonly known as baker's or budding yeast...
  11. ncbi Stochastic switching as a survival strategy in fluctuating environments
    Murat Acar
    Department of Physics, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Nat Genet 40:471-5. 2008
    ..explore how switching affects population growth by using the galactose utilization network of Saccharomyces cerevisiae. We engineered a strain that randomly transitions between two phenotypes as a result of stochastic gene ..
  12. ncbi A global protein kinase and phosphatase interaction network in yeast
    Ashton Breitkreutz
    Centre for Systems Biology, Samuel Lunenfeld Research Institute, 600 University Avenue, Toronto, Ontario, M5G 1X5, Canada
    Science 328:1043-6. 2010
    ..An extensive backbone of kinase-kinase interactions cross-connects the proteome and may serve to coordinate diverse cellular responses...
  13. ncbi The evolutionary fate and consequences of duplicate genes
    M Lynch
    Department of Biology, University of Oregon, Eugene, OR 97403, USA
    Science 290:1151-5. 2000
    ..Although duplicate genes may only rarely evolve new functions, the stochastic silencing of such genes may play a significant role in the passive origin of new species...
  14. ncbi Small molecule activators of sirtuins extend Saccharomyces cerevisiae lifespan
    Konrad T Howitz
    BIOMOL Research Laboratories, Inc, 5120 Butler Pike, Plymouth Meeting, Pennsylvania 19462, USA
    Nature 425:191-6. 2003
    ..In the budding yeast Saccharomyces cerevisiae, calorie restriction extends lifespan by increasing the activity of Sir2 (ref...
  15. ncbi A barrier nucleosome model for statistical positioning of nucleosomes throughout the yeast genome
    Travis N Mavrich
    Center for Gene Regulation, Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania 16802, USA
    Genome Res 18:1073-83. 2008
    ..3' NFRs may be important for transcription termination and anti-sense initiation. We present a high-resolution genome-wide map of TFIIB locations that implicates 3' NFRs in gene looping...
  16. ncbi The SIR2/3/4 complex and SIR2 alone promote longevity in Saccharomyces cerevisiae by two different mechanisms
    M Kaeberlein
    Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Genes Dev 13:2570-80. 1999
    ..Sir2p is a limiting component in promoting yeast longevity, and increasing the gene dosage extends the life span in wild-type cells. A possible role of the conserved SIR2 in mammalian aging is discussed...
  17. ncbi Nonfilamentous C. albicans mutants are avirulent
    H J Lo
    Whitehead Institute for Biomedical Research and Department of Biology, Massachusetts Institute of Technology, Cambridge 02142, USA
    Cell 90:939-49. 1997
    Candida albicans and Saccharomyces cerevisiae switch from a yeast to a filamentous form. In Saccharomyces, this switch is controlled by two regulatory proteins, Ste12p and Phd1p...
  18. ncbi Rewiring of genetic networks in response to DNA damage
    Sourav Bandyopadhyay
    Department of Medicine, University of California, San Diego, La Jolla, CA 92093, USA
    Science 330:1385-9. 2010
    ..Differential networks chart a new type of genetic landscape that is invaluable for mapping cellular responses to stimuli...
  19. ncbi Global analysis of protein expression in yeast
    Sina Ghaemmaghami
    Howard Hughes Medical Institute, University of California-San Francisco, San Francisco, California 94143-2240, USA
    Nature 425:737-41. 2003
    ..To facilitate global protein analyses, we have created a Saccharomyces cerevisiae fusion library where each open reading frame is tagged with a high-affinity epitope and expressed from ..
  20. ncbi Extending healthy life span--from yeast to humans
    Luigi Fontana
    Division of Geriatrics and Nutritional Science, Washington University School of Medicine, St Louis, MO 63110, USA
    Science 328:321-6. 2010
    ..We discuss these findings and their potential application to prevention of age-related disease and promotion of healthy aging in humans, and the challenge of possible negative side effects...
  21. ncbi Nucleosome positioning and gene regulation: advances through genomics
    Cizhong Jiang
    Center for Eukaryotic Gene Regulation, Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, Pennsylvania 16802, USA
    Nat Rev Genet 10:161-72. 2009
    ..A detailed picture is starting to emerge of how diverse factors, including underlying DNA sequences and chromatin remodelling complexes, influence nucleosome positioning...
  22. ncbi An evolutionarily conserved mechanism for controlling the efficiency of protein translation
    Tamir Tuller
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
    Cell 141:344-54. 2010
    ..We suggest that the slow "ramp" at the beginning of mRNAs serves as a late stage of translation initiation, forming an optimal and robust means to reduce ribosomal traffic jams, thus minimizing the cost of protein expression...
  23. ncbi Lessons on longevity from budding yeast
    Matt Kaeberlein
    Department of Pathology, University of Washington, Seattle, Washington 98195, USA
    Nature 464:513-9. 2010
    ..The first interventions to slow human ageing may spring from the humble yeast...
  24. ncbi Dissection of genetically complex traits with extremely large pools of yeast segregants
    Ian M Ehrenreich
    Lewis Sigler Institute for Integrative Genomics, Princeton University, Princeton, New Jersey 08540, USA
    Nature 464:1039-42. 2010
    ..The method involves generating very large numbers of progeny from a cross between two Saccharomyces cerevisiae strains and then phenotyping and genotyping pools of these offspring...
  25. ncbi The DNA-encoded nucleosome organization of a eukaryotic genome
    Noam Kaplan
    Department of Computer Science and Applied Mathematics, Weizmann Institute of Science, Rehovot 76100, Israel
    Nature 458:362-6. 2009
    ..Our results indicate that the intrinsic DNA sequence preferences of nucleosomes have a central role in determining the organization of nucleosomes in vivo...
  26. ncbi Sensing DNA damage through ATRIP recognition of RPA-ssDNA complexes
    Lee Zou
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Science 300:1542-8. 2003
    ..Our data suggest that RPA-coated ssDNA is the critical structure at sites of DNA damage that recruits the ATR-ATRIP complex and facilitates its recognition of substrates for phosphorylation and the initiation of checkpoint signaling...
  27. ncbi Elevated histone expression promotes life span extension
    Jason Feser
    Department of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, Aurora, CO 80010, USA
    Mol Cell 39:724-35. 2010
    ..This study indicates that maintenance of the fundamental chromatin structure is critical for slowing down the aging process and reveals that increasing the histone supply extends life span...
  28. ncbi High-resolution DNA-binding specificity analysis of yeast transcription factors
    Cong Zhu
    Division of Genetics, Department of Medicine, Brigham and Women s Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA
    Genome Res 19:556-66. 2009
    ..These approaches could be adapted to identify TFs and cis regulatory elements in higher eukaryotes...
  29. ncbi Bridging high-throughput genetic and transcriptional data reveals cellular responses to alpha-synuclein toxicity
    Esti Yeger-Lotem
    Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Nat Genet 41:316-23. 2009
    ..Bridging these data and data from mRNA profiling provided functional explanations for many of these genes and identified previously unknown relations between alpha-synuclein toxicity and basic cellular pathways...
  30. ncbi A genetic interaction map of RNA-processing factors reveals links between Sem1/Dss1-containing complexes and mRNA export and splicing
    Gwendolyn M Wilmes
    Department of Biochemistry and Biophysics, University of California, San Francisco, 600 16th Street, Genentech Hall, San Francisco, CA 94143 2200, USA
    Mol Cell 32:735-46. 2008
    ..Finally, we show that Csn12 plays a role in pre-mRNA splicing, which is independent of other signalosome components. Thus, Sem1 is involved in three separate and functionally distinct complexes...
  31. ncbi Mistranslation-induced protein misfolding as a dominant constraint on coding-sequence evolution
    D Allan Drummond
    FAS Center for Systems Biology, Harvard University, Cambridge, MA 02138, USA
    Cell 134:341-52. 2008
    ....
  32. ncbi Histone H3 methylation by Set2 directs deacetylation of coding regions by Rpd3S to suppress spurious intragenic transcription
    Michael J Carrozza
    Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA
    Cell 123:581-92. 2005
    ..This erases transcription elongation-associated acetylation to suppress intragenic transcription initiation...
  33. ncbi Two TOR complexes, only one of which is rapamycin sensitive, have distinct roles in cell growth control
    Robbie Loewith
    Division of Biochemistry, Biozentrum, University of Basel, Klingelbergstrasse 70, CH 4056 Basel, Switzerland
    Mol Cell 10:457-68. 2002
    The target of rapamycin (TOR) proteins in Saccharomyces cerevisiae, TOR1 and TOR2, redundantly regulate growth in a rapamycin-sensitive manner...
  34. ncbi Cse4 is part of an octameric nucleosome in budding yeast
    Raymond Camahort
    Stowers Institute for Medical Research, Kansas City, MO 64110, USA
    Mol Cell 35:794-805. 2009
    ..Taken together, our experimental evidence supports the model that the Cse4 nucleosome is an octamer, containing two copies each of Cse4, H2A, H2B, and H4...
  35. ncbi Cryo-EM structure and rRNA model of a translating eukaryotic 80S ribosome at 5.5-A resolution
    Jean Paul Armache
    Gene Center, Department of Biochemistry, Ludwig Maximilians Universitat Munchen, Feodor Lynen Strasse 25, 81377 Munich, Germany
    Proc Natl Acad Sci U S A 107:19748-53. 2010
    ..5-Å resolution. This map, together with a 6.1-Å map of a Saccharomyces cerevisiae 80S ribosome, has enabled us to model ∼98% of the rRNA...
  36. ncbi Stress signaling from the lumen of the endoplasmic reticulum: coordination of gene transcriptional and translational controls
    R J Kaufman
    Department of Biological Chemistry and Howard Hughes Medical Institute, The University of Michigan Medical Center, Ann Arbor, Michigan 48109-0650 USA
    Genes Dev 13:1211-33. 1999
  37. ncbi GENECODIS: a web-based tool for finding significant concurrent annotations in gene lists
    Pedro Carmona-Saez
    Biocomputing Unit, National Center of Biotechnology CNB CSIC, C Darwin 3, Campus Universidad Autonoma de Madrid, 28049 Madrid, Spain
    Genome Biol 8:R3. 2007
    ..GENECODIS is publicly available at http://genecodis.dacya.ucm.es/...
  38. ncbi Translation efficiency is determined by both codon bias and folding energy
    Tamir Tuller
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
    Proc Natl Acad Sci U S A 107:3645-50. 2010
    ..Using the Escherichia coli and Saccharomyces cerevisiae transcriptomes, we conducted a genome-scale study aiming at dissecting the determinants of translation ..
  39. ncbi A hierarchical combination of factors shapes the genome-wide topography of yeast meiotic recombination initiation
    Jing Pan
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA
    Cell 144:719-31. 2011
    ..This map illuminates the occurrence of DSBs in repetitive DNA elements, repair of which can lead to chromosomal rearrangements. We also discuss implications for evolutionary dynamics of recombination hot spots...
  40. ncbi Unstable tandem repeats in promoters confer transcriptional evolvability
    Marcelo D Vinces
    FAS Center for Systems Biology, Harvard University, 52 Oxford Street, Cambridge, MA 02138, USA
    Science 324:1213-6. 2009
    ..A search for tandem repeats in the Saccharomyces cerevisiae genome revealed that the nucleosome-free region directly upstream of genes (the promoter region) is ..
  41. ncbi Two strategies for gene regulation by promoter nucleosomes
    Itay Tirosh
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
    Genome Res 18:1084-91. 2008
    ..Analysis of nucleosome positioning in human promoters reproduces the main observations. Our results suggest two distinct strategies for gene regulation by chromatin, which are selectively employed by different genes...
  42. ncbi Cell wall integrity signaling in Saccharomyces cerevisiae
    David E Levin
    Department of Biochemistry and Molecular Biology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD 21205, USA
    Microbiol Mol Biol Rev 69:262-91. 2005
    ....
  43. ncbi Mechanism of eukaryotic homologous recombination
    Joseph San Filippo
    Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, CT 06520, USA
    Annu Rev Biochem 77:229-57. 2008
    ..Recent progress on elucidating the mechanisms of action of Rad51 and Dmc1 and their cohorts of ancillary factors is reviewed here...
  44. ncbi Induction of autophagy by spermidine promotes longevity
    Tobias Eisenberg
    Institute of Molecular Biosciences, University of Graz, 8010 Graz, Austria
    Nat Cell Biol 11:1305-14. 2009
    ..Finally, we found that enhanced autophagy is crucial for polyamine-induced suppression of necrosis and enhanced longevity...
  45. ncbi Aneuploidy confers quantitative proteome changes and phenotypic variation in budding yeast
    Norman Pavelka
    Stowers Institute for Medical Research, 1000 East 50th Street, Kansas City, Missouri 64110, USA
    Nature 468:321-5. 2010
    ....
  46. ncbi An ER-mitochondria tethering complex revealed by a synthetic biology screen
    Benoît Kornmann
    Department of Biochemistry and Biophysics, University of California at San Francisco, San Francisco, CA 94158, USA
    Science 325:477-81. 2009
    ..The tethering complex localized to discrete foci, suggesting that discrete sites of close apposition between ER and mitochondria facilitate interorganelle calcium and phospholipid exchange...
  47. ncbi Antisense RNA stabilization induces transcriptional gene silencing via histone deacetylation in S. cerevisiae
    Jurgi Camblong
    Department of Cell Biology, Sciences III, University of Geneva, 30 quai E Ansermet, 1211 Geneva 4, Switzerland
    Cell 131:706-17. 2007
    ....
  48. ncbi The transmembrane kinase Ire1p is a site-specific endonuclease that initiates mRNA splicing in the unfolded protein response
    C Sidrauski
    Department of Biochemistry and Biophysics, University of California, School of Medicine, San Francisco 94143 0448, USA
    Cell 90:1031-9. 1997
    ..The addition of purified tRNA ligase completes splicing; we therefore have reconstituted HAC1 mRNA splicing in vitro from purified components...
  49. ncbi MMEJ repair of double-strand breaks (director's cut): deleted sequences and alternative endings
    Mitch McVey
    Department of Biology, Tufts University, 165 Packard Avenue, Medford, MA 02155, USA
    Trends Genet 24:529-38. 2008
    ..We propose a mechanistic model for MMEJ and highlight important questions for future research...
  50. ncbi A comprehensive strategy enabling high-resolution functional analysis of the yeast genome
    David K Breslow
    Department of Cellular and Molecular Pharmacology, University of California, San Francisco, 1700 4th Street, San Francisco, California 94158, USA
    Nat Methods 5:711-8. 2008
    Functional genomic studies in Saccharomyces cerevisiae have contributed enormously to our understanding of cellular processes...
  51. ncbi Full dynamic range proteome analysis of S. cerevisiae by targeted proteomics
    Paola Picotti
    Institute of Molecular Systems Biology, ETH Zurich, Zurich CH 8093, Switzerland
    Cell 138:795-806. 2009
    ..We therefore demonstrate the potential of SRM-based proteomics to provide assays for the measurement of any set of proteins of interest in yeast at high-throughput and quantitative accuracy...
  52. ncbi Systematic mapping of genetic interaction networks
    Scott J Dixon
    Banting and Best Department of Medical Research, Terrence Donnelly Center for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario M5S 1A7, Canada
    Annu Rev Genet 43:601-25. 2009
    ..Systematic screens conducted in the yeast Saccharomyces cerevisiae have identified thousands of genetic interactions and provided insight into the global structure of ..
  53. ncbi Evolution of biomolecular networks: lessons from metabolic and protein interactions
    Takuji Yamada
    European Molecular Biology Laboratory, Heidelberg, Germany
    Nat Rev Mol Cell Biol 10:791-803. 2009
    ..However, many evolutionary constraints can be uncovered only if temporal and spatial aspects are included in the network analysis...
  54. ncbi Specificity and stability in topology of protein networks
    Sergei Maslov
    Department of Physics, Brookhaven National Laboratory, Upton, NY 11973, USA
    Science 296:910-3. 2002
    ..This effect decreases the likelihood of cross talk between different functional modules of the cell and increases the overall robustness of a network by localizing effects of deleterious perturbations...
  55. ncbi Systematic genetic analysis with ordered arrays of yeast deletion mutants
    A H Tong
    Banting and Best Department of Medical Research, University of Toronto, Toronto ON, Canada M5G 1L6
    Science 294:2364-8. 2001
    In Saccharomyces cerevisiae, more than 80% of the approximately 6200 predicted genes are nonessential, implying that the genome is buffered from the phenotypic consequences of genetic perturbation...
  56. ncbi Translational regulation of GCN4 and the general amino acid control of yeast
    Alan G Hinnebusch
    Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, Bethesda, Maryland 20892, USA
    Annu Rev Microbiol 59:407-50. 2005
    ..The activator protein Gcn4 of the yeast Saccharomyces cerevisiae is regulated by an intricate translational control mechanism, which is the primary focus of this review, ..
  57. ncbi Kinetochore recruitment of two nucleolar proteins is required for homolog segregation in meiosis I
    Kirsten P Rabitsch
    Research Institute of Molecular Pathology, Dr Bohr Gasse 7, A 1030 Vienna, Austria
    Dev Cell 4:535-48. 2003
    ..of a protein complex, Csm1/Lrs4, that is essential for monoorientation of sister kinetochores in Saccharomyces cerevisiae. Both proteins are present in vegetative cells, where they reside in the nucleolus...
  58. ncbi Increased mutagenesis and unique mutation signature associated with mitotic gene conversion
    Wade M Hicks
    Department of Biology and Rosenstiel Center, Brandeis University, Waltham, MA 02454 9110, USA
    Science 329:82-5. 2010
    To examine the fidelity of DNA synthesis during double-strand break (DSB) repair in Saccharomyces cerevisiae we studied gene conversion in which both strands of DNA are newly synthesized...
  59. ncbi Identification of aneuploidy-tolerating mutations
    Eduardo M Torres
    David H Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Cell 143:71-83. 2010
    ....
  60. ncbi Uniform transitions of the general RNA polymerase II transcription complex
    Andreas Mayer
    Gene Center Munich and Department of Biochemistry, Center for Integrated Protein Science, Ludwig Maximilians Universitat Munchen, Munich, Germany
    Nat Struct Mol Biol 17:1272-8. 2010
    ..Transitions are uniform and independent of gene length, type and expression...
  61. ncbi SIR2 and SIR4 interactions differ in core and extended telomeric heterochromatin in yeast
    S Strahl-Bolsinger
    Department of Biological Chemistry, University of California, Los Angeles 90095, USA
    Genes Dev 11:83-93. 1997
    ..RAP1 binding at the core region is unaffected by SIR3 overproduction and RAP1 shows no evidence of spreading. Thus, we propose that the structure of core telomeric heterochromatin differs from that extended by SIR3...
  62. ncbi A library of yeast transcription factor motifs reveals a widespread function for Rsc3 in targeting nucleosome exclusion at promoters
    Gwenael Badis
    Banting and Best Department of Medical Research, University of Toronto, Toronto, ON M5S 3E1, Canada
    Mol Cell 32:878-87. 2008
    ....
  63. ncbi A ubiquitin-like system mediates protein lipidation
    Y Ichimura
    Department of Cell Biology, National Institute for Basic Biology, Okazaki, Japan
    Nature 408:488-92. 2000
    ..11) and Apg3, respectively. These reactions are necessary for the formation of Apg8-phosphatidylethanolamine. This lipidation has an essential role in membrane dynamics during autophagy...
  64. ncbi Prevalent positive epistasis in Escherichia coli and Saccharomyces cerevisiae metabolic networks
    Xionglei He
    Department of Ecology and Evolutionary Biology, Ann Arbor, Michigan, USA
    Nat Genet 42:272-6. 2010
    ..interactions amongst biochemical reactions within the metabolic networks of Escherichia coli and Saccharomyces cerevisiae using flux balance analysis...
  65. ncbi Comprehensive characterization of genes required for protein folding in the endoplasmic reticulum
    Martin C Jonikas
    Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94143, USA
    Science 323:1693-7. 2009
    ..The use of a quantitative reporter in a comprehensive screen followed by systematic analysis of genetic dependencies should be broadly applicable to functional dissection of complex cellular processes from yeast to human...
  66. ncbi Abundance of ribosomal RNA gene copies maintains genome integrity
    Satoru Ide
    National Institute of Genetics, SOKENDAI, 1111 Yata, Mishima, 411 8540 Japan
    Science 327:693-6. 2010
    ..These results suggest that high concentrations of heavily transcribed genes are toxic to the cells, and therefore amplified genes, such as rDNA, have evolved...
  67. ncbi N-terminal acetylation of cellular proteins creates specific degradation signals
    Cheol Sang Hwang
    Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA
    Science 327:973-7. 2010
    ..Using the yeast Saccharomyces cerevisiae, we found that the Nt-acetylated Met residue could act as a degradation signal (degron), targeted by the ..
  68. ncbi Schizosaccharomyces pombe genome-wide nucleosome mapping reveals positioning mechanisms distinct from those of Saccharomyces cerevisiae
    Alexandra B Lantermann
    Adolf Butenandt Institut, University of Munich, Munich, Germany
    Nat Struct Mol Biol 17:251-7. 2010
    ..differences from the previously published nucleosome organization of the distantly related yeast Saccharomyces cerevisiae. DNA sequence guides nucleosome positioning differently: for example, poly(dA-dT) elements are not ..
  69. ncbi Global analysis of nascent RNA reveals transcriptional pausing in terminal exons
    Fernando Carrillo Oesterreich
    Max Planck Institute of Molecular Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany
    Mol Cell 40:571-81. 2010
    ..The discovery of terminal exon pausing demonstrates functional coupling of transcription and splicing near gene ends...
  70. ncbi Frequent gain and loss of functional transcription factor binding sites
    Scott W Doniger
    Computational Biology Program, Washington University School of Medicine, St Louis, Missouri, United States of America
    PLoS Comput Biol 3:e99. 2007
    ..The frequent gain and loss of TFBSs implies that cis-regulatory sequences are labile and, in the absence of turnover, may contribute to species-specific patterns of gene expression...
  71. ncbi A consensus of core protein complex compositions for Saccharomyces cerevisiae
    Joris J Benschop
    Department of Physiological Chemistry, University Medical Centre Utrecht, 3584 CG Utrecht, The Netherlands
    Mol Cell 38:916-28. 2010
    ..We determine consensus compositions for 409 core protein complexes from Saccharomyces cerevisiae by merging previous predictions with a new approach...
  72. ncbi Localization of eukaryote-specific ribosomal proteins in a 5.5-Å cryo-EM map of the 80S eukaryotic ribosome
    Jean Paul Armache
    Gene Center, Department of Biochemistry, Ludwig Maximilians Universitat Munchen, Feodor Lynen Strasse 25, 81377 Munich, Germany
    Proc Natl Acad Sci U S A 107:19754-9. 2010
    ..5-Å resolution, together with a 6.1-Å map of a translating Saccharomyces cerevisiae 80S ribosome, we have localized and modeled 74/80 (92...
  73. ncbi Estimating the per-base-pair mutation rate in the yeast Saccharomyces cerevisiae
    Gregory I Lang
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    Genetics 178:67-82. 2008
    ..44x10(-10) at CAN1) and we propose a definition for the effective target size of genes (the probability that a mutation inactivates the gene) that acknowledges that the mutation rate is nonuniform across the genome...
  74. ncbi The AAA ATPase Cdc48/p97 and its partners transport proteins from the ER into the cytosol
    Y Ye
    Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 414:652-6. 2001
    ..We propose that the Cdc48/p97-Ufd1-Npl4 complex extracts proteins from the ER membrane for cytosolic degradation...
  75. ncbi How common are extraribosomal functions of ribosomal proteins?
    Jonathan R Warner
    Department of Cell Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Mol Cell 34:3-11. 2009
    ..Is this due to a lack of imaginative evolution by cells and viruses, or to a lack of imaginative experiments by molecular biologists?..
  76. ncbi The biomass objective function
    Adam M Feist
    GT Life Sciences, Inc, 10520 Wateridge Circle, San Diego, CA 92122, USA
    Curr Opin Microbiol 13:344-9. 2010
    ..Here we review fundamental issues associated with its formulation and use to compute optimal growth states...
  77. ncbi Genome-wide views of chromatin structure
    Oliver J Rando
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Annu Rev Biochem 78:245-71. 2009
    ....
  78. ncbi tRNA cleavage is a conserved response to oxidative stress in eukaryotes
    Debrah M Thompson
    Department of Molecular and Cellular Biology, University of Arizona, Tucson, Arizona 85721, USA
    RNA 14:2095-103. 2008
    ..In this work, we demonstrate that Saccharomyces cerevisiae contains a small RNA population consisting primarily of tRNA halves and rRNA fragments...
  79. ncbi Histone H4 lysine 16 acetylation regulates cellular lifespan
    Weiwei Dang
    Gene Expression and Regulation Program, The Wistar Institute Philadelphia, Pennsylvania 19104, USA
    Nature 459:802-7. 2009
    ..This pathway, distinct from existing ageing models for yeast, may represent an evolutionarily conserved function of sirtuins in regulation of replicative ageing by maintenance of intact telomeric chromatin...
  80. ncbi The glyoxylate cycle is required for fungal virulence
    M C Lorenz
    Whitehead Institute for Biomedical Research, Nine Cambridge Center, Massachusetts 02142, USA
    Nature 412:83-6. 2001
    ..Here we use genome-wide expression profiles of the related yeast Saccharomyces cerevisiae to obtain a signature of the events that take place in the fungus on ingestion by a mammalian macrophage...
  81. ncbi Atg8, a ubiquitin-like protein required for autophagosome formation, mediates membrane tethering and hemifusion
    Hitoshi Nakatogawa
    Department of Cell Biology, National Institute for Basic Biology, Okazaki 444 8585, Japan
    Cell 130:165-78. 2007
    ..Atg8 is a ubiquitin-like protein required for this process in Saccharomyces cerevisiae that can be conjugated to the lipid phosphatidylethanolamine by a ubiquitin-like system...
  82. ncbi Deciphering protein kinase specificity through large-scale analysis of yeast phosphorylation site motifs
    Janine Mok
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520, USA
    Sci Signal 3:ra12. 2010
    ..approach to determine consensus phosphorylation site motifs targeted by 61 of the 122 kinases in Saccharomyces cerevisiae. By correlating these motifs with kinase primary sequence, we uncovered previously unappreciated rules ..
  83. ncbi Ataxin-2 intermediate-length polyglutamine expansions are associated with increased risk for ALS
    Andrew C Elden
    Department of Cell and Developmental Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Nature 466:1069-75. 2010
    ..Furthermore, these findings indicate that the TDP-43-ATXN2 interaction may be a promising target for therapeutic intervention in ALS and other TDP-43 proteinopathies...
  84. ncbi Defining genetic interaction
    Ramamurthy Mani
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 250 Longwood Avenue, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 105:3461-6. 2008
    ..Although 52% of known synergistic genetic interactions in Saccharomyces cerevisiae were inferred according to the Min definition, we find that both Product and Log definitions (shown here ..
  85. ncbi An E3 ubiquitin ligase prevents ectopic localization of the centromeric histone H3 variant via the centromere targeting domain
    Prerana Ranjitkar
    Division of Basic Sciences, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue N, P O Box 19024, Seattle, WA 98109, USA
    Mol Cell 40:455-64. 2010
    ..Taken together, we propose that the CATD has a previously unknown role in maintaining the exclusive localization of Cse4 by preventing its mislocalization to euchromatin via Psh1-mediated degradation...
  86. ncbi Genome-wide patterns of histone modifications in yeast
    Catherine B Millar
    Department of Biological Chemistry, Geffen School of Medicine and the Molecular Biology Institute, University of California, Los Angeles, California 90095, USA
    Nat Rev Mol Cell Biol 7:657-66. 2006
    ..Recent studies have mapped histone modifications across the Saccharomyces cerevisiae genome...
  87. ncbi Origin and evolution of the mitochondrial proteome
    C G Kurland
    Department of Molecular Evolution, Evolutionary Biology Centre, University of Uppsala, Uppsala SE 752 36, Lund University, Lund SE 223 62, Sweden
    Microbiol Mol Biol Rev 64:786-820. 2000
    ..There are no indications of a specific alpha-proteobacterial origin to genes for glycolysis. In the absence of data to the contrary, it is assumed that the ancestral host cell was a heterotroph...
  88. ncbi Nascent transcript sequencing visualizes transcription at nucleotide resolution
    L Stirling Churchman
    Department of Cellular and Molecular Pharmacology, Howard Hughes Medical Institute, University of California, San Francisco, USA
    Nature 469:368-73. 2011
    ..Application of NET-seq in Saccharomyces cerevisiae reveals that although promoters are generally capable of divergent transcription, the Rpd3S deacetylation ..
  89. ncbi Modelling neurodegeneration in Saccharomyces cerevisiae: why cook with baker's yeast?
    Vikram Khurana
    Department of Neurology, Brigham and Women s and Massachusetts General Hospitals, Harvard Medical School, Boston, Massachusetts, USA
    Nat Rev Neurosci 11:436-49. 2010
    ..Here, we describe why the budding yeast Saccharomyces cerevisiae has a unique role in the neurodegeneration armamentarium...
  90. ncbi Structure and function of a transcriptional network activated by the MAPK Hog1
    Andrew P Capaldi
    Howard Hughes Medical Institute, Harvard University Faculty of Arts and Sciences Center for Systems Biology, Department of Molecular and Cellular Biology, Cambridge, Massachusetts 02138, USA
    Nat Genet 40:1300-6. 2008
    ..This study lays out a path to identifying and characterizing the role of signal integration and processing in other gene regulatory networks...
  91. ncbi RNA polymerase II-TFIIB structure and mechanism of transcription initiation
    Dirk Kostrewa
    Gene Center Munich and Center for Integrated Protein Science Munich CIPSM, Department of Chemistry and Biochemistry, Ludwig Maximilians Universitat Munchen, Feodor Lynen Strasse 25, 81377 Munich, Germany
    Nature 462:323-30. 2009
    ..Synthesis of the RNA chain and rewinding of upstream DNA displace the B-reader and B-linker, respectively, to trigger B release and elongation complex formation...
  92. ncbi How do Cdc7 and cyclin-dependent kinases trigger the initiation of chromosome replication in eukaryotic cells?
    Karim Labib
    Cancer Research UK, Paterson Institute for Cancer Research, University of Manchester, Manchester M20 4BX, United Kingdom
    Genes Dev 24:1208-19. 2010
    ..A series of recent studies has shed new light on the targets of Cdc7 and CDK, indicating that chromosome replication probably initiates by a fundamentally similar mechanism in all eukaryotes...
  93. ncbi A yeast TDP-43 proteinopathy model: Exploring the molecular determinants of TDP-43 aggregation and cellular toxicity
    Brian S Johnson
    Department of Cell and Developmental Biology, University of Pennsylvania, Philadelphia, PA 19104, USA
    Proc Natl Acad Sci U S A 105:6439-44. 2008
    ..This work provides a mechanistic framework for investigating the toxicity of TDP-43 aggregation relevant to human disease and establishes a manipulable, high-throughput model for discovering potential therapeutic strategies...
  94. ncbi Growth signaling promotes chronological aging in budding yeast by inducing superoxide anions that inhibit quiescence
    Martin Weinberger
    Department of Molecular and Cellular Biology, Roswell Park Cancer Institute, Buffalo, NY 14263, USA
    Aging (Albany NY) 2:709-26. 2010
    ..Here we report that in Saccharomyces cerevisiae, alterations in growth signaling pathways impact levels of superoxide anions that promote chronological ..
  95. ncbi Signalling pathways in the unfolded protein response: development from yeast to mammals
    Kazutoshi Mori
    Department of Biophysics, Graduate School of Science, Kyoto University, Kitashirakawa Oiwake, Sakyo ku, Kyoto 606 8502, Japan
    J Biochem 146:743-50. 2009
    ..functional sensors/transducers, ubiquitously expressed, has increased with evolution, for example, one in Saccharomyces cerevisiae, two in Caenorhabditis elegans and Drosophila melanogaster, and three in mammals...
  96. ncbi Storage lipid synthesis is non-essential in yeast
    Line Sandager
    Department of Crop Science, Swedish University of Agricultural Sciences, Box 44, S 230 53 Alnarp, Sweden
    J Biol Chem 277:6478-82. 2002
    Steryl esters and triacylglycerol (TAG) are the main storage lipids in eukaryotic cells. In the yeast Saccharomyces cerevisiae, these storage lipids accumulate during stationary growth phase within organelles known as lipid bodies...
  97. ncbi Genetic basis of individual differences in the response to small-molecule drugs in yeast
    Ethan O Perlstein
    Howard Hughes Medical Institute, Broad Institute of Harvard and MIT, 7 Cambridge Center, Cambridge, Massachusetts 02142, USA
    Nat Genet 39:496-502. 2007
    ..Our results provide a step toward a systematic understanding of small-molecule drug action in genetically distinct individuals...
  98. ncbi TDP-43 mediates degeneration in a novel Drosophila model of disease caused by mutations in VCP/p97
    Gillian P Ritson
    Department of Neurology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
    J Neurosci 30:7729-39. 2010
    ..VCP (p97 in mouse, TER94 in Drosophila melanogaster, and CDC48 in Saccharomyces cerevisiae) is a highly conserved AAA(+) (ATPases associated with multiple cellular activities) ATPase that ..
  99. ncbi Characterization of mutations in NOT2 indicates that it plays an important role in maintaining the integrity of the CCR4-NOT complex
    Pamela Russell
    Department of Biochemistry and Molecular Biology, University of New Hampshire, Durham 03824, USA
    J Mol Biol 322:27-39. 2002
    ..of the CCR4-NOT complex that plays multiple roles in the regulation of mRNA production in the yeast Saccharomyces cerevisiae. We have identified four novel not2 mutations and have characterized these and two previously described ..
  100. ncbi Contribution of Msh2 and Msh6 subunits to the asymmetric ATPase and DNA mismatch binding activities of Saccharomyces cerevisiae Msh2-Msh6 mismatch repair protein
    Edwin Antony
    Molecular Biology and Biochemistry Department, Wesleyan University, Middletown, CT 06459, USA
    DNA Repair (Amst) 5:153-62. 2006
    Previous analyses of both Thermus aquaticus MutS homodimer and Saccharomyces cerevisiae Msh2-Msh6 heterodimer have revealed that the subunits in these protein complexes bind and hydrolyze ATP asymmetrically, emulating their asymmetric DNA ..
  101. ncbi Ssn6, an important factor of morphological conversion and virulence in Candida albicans
    Cheol-Sang Hwang
    Laboratory of Biophysics, School of Biological Sciences, and Institute of Microbiology, Seoul National University, Seoul 151-742, Republic of Korea
    Mol Microbiol 47:1029-43. 2003
    ..namely SSN6, encoding a putative global transcriptional co-repressor that is highly homologous to the Saccharomyces cerevisiae Ssn6. The isolated C. albicans SSN6 complemented the pleiotropic phenotypes of S...

Research Grants81

  1. Role of the OPI1 gene in controlling viability of Candida glabrata
    TODD REYNOLDS; Fiscal Year: 2007
    ..CgOpi1p is similar to the Opi1p protein in the bakers' yeast Saccharomyces cerevisiae. S...
  2. ENZYMATIC MECHANISMS OF GENETIC RECOMBINATION
    Richard D Kolodner; Fiscal Year: 2010
    ..In the proposed studies, Saccharomyces cerevisiae will be used as a model system to identify the genes and pathways that act to suppress GCRs...
  3. INTEGRATION SPECIFICITY OF THE TY3 RETROTRANSPOSON
    Suzanne Sandmeyer; Fiscal Year: 1999
    ..TFIIIC suppressors identified in the Sentenac lab will be analyzed for affects on Ty3 transposition. As residues in Ty3 are identified which are critical to targeting, suppressor screens for interacting host factors will be performed. ..
  4. HTLV-I Tax activates the anaphase promoting complex
    Chou Zen Giam; Fiscal Year: 2009
    ....
  5. MECHANISM OF PRE-MRNA SPLICING
    MAGDA KONARSKA; Fiscal Year: 2009
    ..By contrast, splice site signals in Saccharomyces cerevisiae fit well to the consensus and alternative splicing is thought not to occur in yeast...
  6. Regulation of nitrogen catabolic gene expression in S cerevisiae
    TERRANCE COOPER; Fiscal Year: 2007
    ..More importantly it will serve as an efficient model system that generates information, much of which will be directly applicable to mammalian cells because the Tor pathway is so well conserved between these organisms. ..
  7. Regulation of nitrogen catabolic gene expression in S cerevisiae
    Terrance G Cooper; Fiscal Year: 2010
    ..More importantly it will serve as an efficient model system that generates information, much of which will be directly applicable to mammalian cells because the Tor pathway is so well conserved between these organisms. ..
  8. MECHANISM OF PRE-MRNA SPLICING
    MAGDA KONARSKA; Fiscal Year: 2010
    ..By contrast, splice site signals in Saccharomyces cerevisiae fit well to the consensus and alternative splicing is thought not to occur in yeast...
  9. MANNOPROTEIN BIOSYNTHESIS IN SACCHAROMYCES CEREVISIAE
    Annette Herscovics; Fiscal Year: 2000
    ..Large quantities of the catalytic domain of the al,2-mannosidase from Saccharomyces cerevisiae that trims Man9GlcNAc2 to Man8GlcNAc2 have been produced in Pichia pastoris...
  10. MECHANISM OF SITE SPECIFIC DNA RECOMBINATION
    Makkuni Jayaram; Fiscal Year: 2001
    ..The tests involve the building synthetic substrates to test both questions. ..
  11. GLYCOPROTEIN BIOSYNTHESIS IN YEAST
    Robert Trimble; Fiscal Year: 1999
    ..The current application proposes in four Aims studies in the small eukaryotes Saccharomyces cerevisiae and schizosaccharomyces pombe to determine the extent to which the substrate specificity of glycan ..
  12. DNA REPLICATION AND CHROMOSOME STRUCTURE IN YEAST
    Virginia A Zakian; Fiscal Year: 2011
    ..These studies will lay the groundwork for the identification and analysis of human proteins with similar functions. ..
  13. DNA REPLICATION AND CHROMOSOME STRUCTURE IN YEAST
    Virginia A Zakian; Fiscal Year: 2010
    ..These studies will lay the groundwork for the identification and analysis of human proteins with similar functions. ..
  14. SORTING AND TRANSPORT OF YEAST MEMBRANE PROTEINS
    Tom Stevens; Fiscal Year: 2009
    ..of the vacuolar-type proton-translocating ATPase (V-ATPase) in the simple model eukaryote, the yeast Saccharomyces cerevisiae. Yeast has proved to be an excellent model system, both for identifying the proteins regulating membrane ..
  15. REGULATION OF RETROTRANSPOSITION IN S. CEREVISIAE
    M Joan Curcio; Fiscal Year: 2011
    ..Cellular proteins that are required for retroviral replication are potential targets for the development of new drugs to treat AIDS. ..
  16. REGULATION OF RETROTRANSPOSITION IN S. CEREVISIAE
    M Joan Curcio; Fiscal Year: 2010
    ..Cellular proteins that are required for retroviral replication are potential targets for the development of new drugs to treat AIDS. ..
  17. MOLECULAR MARKER FOR INVASIVE CANDIDA ALBICANS
    MARGARET HOSTETTER; Fiscal Year: 2001
    ..albicans and thereby bring forward new strategies for the control of candidal infection. ..
  18. FUNCTION OF AXR1 AND AXR1 LIKE GENES IN ARABIDOPSIS
    Mark Estelle; Fiscal Year: 2000
    ..is to determine the biochemical and cellular function of the AXR1 family of proteins in Arabidopsis and Saccharomyces cerevisiae. This family of proteins is related to the ubiquitin-activating enzyme (E1) and genetic studies have ..
  19. Methods for engineering S. cerevisiae strains carrying multiple precise deletions
    FREDERICK ROTH; Fiscal Year: 2007
    ..Multiply-deleted strains allow functional study of genes in gene families, while multiply-inserted strains allow the study of human protein complexes in a tractable model organism. ..
  20. ROLE OF PROTEIN KINASE C IN YEAST GROWTH CONTROL
    David Levin; Fiscal Year: 1993
    ..Other known cell lysis mutants will also be examined for genetic interactions with PKC1 and BCK1...
  21. SORTING AND TRANSPORT OF YEAST MEMBRANE PROTEINS
    TOM HALL STEVENS; Fiscal Year: 2010
    ..of the vacuolar-type proton-translocating ATPase (V-ATPase) in the simple model eukaryote, the yeast Saccharomyces cerevisiae. Yeast has proved to be an excellent model system, both for identifying the proteins regulating membrane ..
  22. Control of Yeast Life Span
    Kurt W Runge; Fiscal Year: 2010
    ..pathway that revealed the importance of the b-subunit of the F1F0-ATP synthase complex, encoded in Saccharomyces cerevisiae by the ATP2 gene...
  23. CONTROL OF ARG-2 GENE EXPRESSION IN NEUROSPORA
    MATTHEW SACHS; Fiscal Year: 2009
    ..AAP-mediated regulation is observed in vivo in both Neurospora crassa and Saccharomyces cerevisiae and in vitro, using fungal, plant and animal extracts...
  24. CONTROL OF ARG-2 GENE EXPRESSION IN NEUROSPORA
    MATTHEW SACHS; Fiscal Year: 2006
    ..AAP-mediated regulation is observed in vivo in both Neurospora crassa and Saccharomyces cerevisiae and in vitro, using fungal, plant and animal extracts...
  25. Assembly and Function of the Yeast Spore Wall
    Aaron M Neiman; Fiscal Year: 2010
    ..The spore wall of Saccharomyces cerevisiae is a complex structure that forms de novo during the process of sporulation...
  26. CONTROL OF ARG-2 GENE EXPRESSION IN NEUROSPORA
    MATTHEW SACHS; Fiscal Year: 2007
    ..AAP-mediated regulation is observed in vivo in both Neurospora crassa and Saccharomyces cerevisiae and in vitro, using fungal, plant and animal extracts...
  27. CONTROL OF ARG-2 GENE EXPRESSION IN NEUROSPORA
    MATTHEW SACHS; Fiscal Year: 2007
    ..AAP-mediated regulation is observed in vivo in both Neurospora crassa and Saccharomyces cerevisiae and in vitro, using fungal, plant and animal extracts...
  28. GENETIC ANALYSIS OF PLEIOTROPIC DRUG RESISTANCE
    W Moye Rowley; Fiscal Year: 2009
    ..We are using the yeast Saccharomyces cerevisiae as a model eukaryotic system that has a well described set of multidrug resistance loci called ..
  29. GENETIC ANALYSIS OF PLEIOTROPIC DRUG RESISTANCE
    W Scott Moye Rowley; Fiscal Year: 2010
    ..We are using the yeast Saccharomyces cerevisiae as a model eukaryotic system that has a well described set of multidrug resistance loci called ..
  30. MANNOPROTEIN BIOSYNTHESIS IN SACCHAROMYCES CEREVISIAE
    Annette Herscovics; Fiscal Year: 2005
    ....
  31. SACCHAROMYCES CEREVISIAE MICROTUBULE CYTOSKELETON
    Georjana Barnes; Fiscal Year: 1993
    Having used biochemical approaches to identify components of the Saccharomyces cerevisiae microtubule cytoskeleton, we will now determine how these components function during mitosis, meiosis, and nuclear fusion...
  32. Arrest, Recovery, and Adaptation from DNA Damage
    James Haber; Fiscal Year: 2009
    We will continue and extend our analysis of the DNA damage checkpoint in the model organism, Saccharomyces cerevisiae. In yeast, as well as in human cells, the DNA damage checkpoint acts to prevent mitosis until DNA has been repaired...
  33. YEAST GENETIC RESEARCH RESOURCE CENTER
    SHUNG CHANG JONG; Fiscal Year: 2002
    ..abstract_text> ..
  34. Molecular Structure of Yeast Chromosome 1
    DAVID KABACK; Fiscal Year: 2005
    We propose to continue functional genomic studies on the model eukaryote Saccharomyces cerevisiae and will investigate a proposed function for subtelomeric DNA that comprises approximately 7 percent of its genome...