aspergillus oryzae

Summary

Summary: An imperfect fungus present on most agricultural seeds and often responsible for the spoilage of seeds in bulk storage. It is also used in the production of fermented food or drink, especially in Japan.

Top Publications

  1. pmc Survey of the transcriptome of Aspergillus oryzae via massively parallel mRNA sequencing
    Bin Wang
    School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, Guangdong, China
    Nucleic Acids Res 38:5075-87. 2010
  2. doi Autophagy delivers misfolded secretory proteins accumulated in endoplasmic reticulum to vacuoles in the filamentous fungus Aspergillus oryzae
    Shinya Kimura
    Department of Biotechnology, The University of Tokyo, Bunkyo ku, Tokyo 113 8657, Japan
    Biochem Biophys Res Commun 406:464-70. 2011
  3. doi Aflatoxin non-productivity of Aspergillus oryzae caused by loss of function in the aflJ gene product
    Takuro Kiyota
    National Research Institute of Brewing, Kagamiyama, Higashihiroshima, Japan
    J Biosci Bioeng 111:512-7. 2011
  4. pmc Purification, characterization, and substrate specificity of a novel highly glucose-tolerant beta-glucosidase from Aspergillus oryzae
    C Riou
    Laboratoire de Microbiologie et Technologie des Fermentations, Institut National de la Recherche Agronomique, Institut des Produits de la Vigne, F 34060 Montpellier cedex 2, France
    Appl Environ Microbiol 64:3607-14. 1998
  5. doi Genes regulated by AoXlnR, the xylanolytic and cellulolytic transcriptional regulator, in Aspergillus oryzae
    Yuji Noguchi
    Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University, Aichi, Japan
    Appl Microbiol Biotechnol 85:141-54. 2009
  6. ncbi Post-genomic insights into the plant polysaccharide degradation potential of Aspergillus nidulans and comparison to Aspergillus niger and Aspergillus oryzae
    Pedro M Coutinho
    AFMB UMR 6098 CNRS Universités Aix Marseille I and II, Case 932, 163 av de Luminy, 13288 Marseille Cedex 9, France
    Fungal Genet Biol 46:S161-S169. 2009
  7. ncbi Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzae
    James E Galagan
    The Broad Institute of MIT and Harvard, 320 Charles Street, Cambridge, Massachusetts 02142, USA
    Nature 438:1105-15. 2005
  8. pmc Novel hydrophobic surface binding protein, HsbA, produced by Aspergillus oryzae
    Shinsaku Ohtaki
    Laboratory of Molecular Enzymology, Department of Molecular and Cell Biology, Graduate School of Agricultural Science, Tohoku University, 1 1 Amamiya, Tsutsumi dori, Aobaku, Sendai 981 8555, Japan
    Appl Environ Microbiol 72:2407-13. 2006
  9. ncbi Aspergillus oryzae laeA regulates kojic acid synthesis genes
    Ken Oda
    Genome Biotechnology Laboratory, Kanazawa Institute of Technology
    Biosci Biotechnol Biochem 75:1832-4. 2011
  10. ncbi Polyol accumulation by Aspergillus oryzae at low water activity in solid-state fermentation
    George J G Ruijter
    Wageningen Centre for Food Sciences, PO Box 557, 6700AN Wageningen, The Netherlands
    Microbiology 150:1095-101. 2004

Detail Information

Publications252 found, 100 shown here

  1. pmc Survey of the transcriptome of Aspergillus oryzae via massively parallel mRNA sequencing
    Bin Wang
    School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, Guangdong, China
    Nucleic Acids Res 38:5075-87. 2010
    b>Aspergillus oryzae, an important filamentous fungus used in food fermentation and the enzyme industry, has been shown through genome sequencing and various other tools to have prominent features in its genomic composition...
  2. doi Autophagy delivers misfolded secretory proteins accumulated in endoplasmic reticulum to vacuoles in the filamentous fungus Aspergillus oryzae
    Shinya Kimura
    Department of Biotechnology, The University of Tokyo, Bunkyo ku, Tokyo 113 8657, Japan
    Biochem Biophys Res Commun 406:464-70. 2011
    ..of misfolded secretory proteins accumulated in endoplasmic reticulum (ER) in the filamentous fungus Aspergillus oryzae. In late-phase cultures, a disulfide bond-deleted mutant of the secretory protein α-amylase AmyB fused with ..
  3. doi Aflatoxin non-productivity of Aspergillus oryzae caused by loss of function in the aflJ gene product
    Takuro Kiyota
    National Research Institute of Brewing, Kagamiyama, Higashihiroshima, Japan
    J Biosci Bioeng 111:512-7. 2011
    b>Aspergillus oryzae, although closely related to Aspergillus flavus, does not produce aflatoxin (AF). A...
  4. pmc Purification, characterization, and substrate specificity of a novel highly glucose-tolerant beta-glucosidase from Aspergillus oryzae
    C Riou
    Laboratoire de Microbiologie et Technologie des Fermentations, Institut National de la Recherche Agronomique, Institut des Produits de la Vigne, F 34060 Montpellier cedex 2, France
    Appl Environ Microbiol 64:3607-14. 1998
    b>Aspergillus oryzae was found to secrete two distinct beta-glucosidases when it was grown in liquid culture on various substrates. The major form had a molecular mass of 130 kDa and was highly inhibited by glucose...
  5. doi Genes regulated by AoXlnR, the xylanolytic and cellulolytic transcriptional regulator, in Aspergillus oryzae
    Yuji Noguchi
    Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University, Aichi, Japan
    Appl Microbiol Biotechnol 85:141-54. 2009
    ..Overexpression of the aoxlnR gene in Aspergillus oryzae (A...
  6. ncbi Post-genomic insights into the plant polysaccharide degradation potential of Aspergillus nidulans and comparison to Aspergillus niger and Aspergillus oryzae
    Pedro M Coutinho
    AFMB UMR 6098 CNRS Universités Aix Marseille I and II, Case 932, 163 av de Luminy, 13288 Marseille Cedex 9, France
    Fungal Genet Biol 46:S161-S169. 2009
    ..potential of Aspergillus nidulans was analysed in detail and compared to that of Aspergillus niger and Aspergillus oryzae using a combination of bioinformatics, physiology and transcriptomics. Manual verification indicated that 28...
  7. ncbi Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzae
    James E Galagan
    The Broad Institute of MIT and Harvard, 320 Charles Street, Cambridge, Massachusetts 02142, USA
    Nature 438:1105-15. 2005
    ..Aspergillus nidulans, and a comparative study with Aspergillus fumigatus, a serious human pathogen, and Aspergillus oryzae, used in the production of sake, miso and soy sauce...
  8. pmc Novel hydrophobic surface binding protein, HsbA, produced by Aspergillus oryzae
    Shinsaku Ohtaki
    Laboratory of Molecular Enzymology, Department of Molecular and Cell Biology, Graduate School of Agricultural Science, Tohoku University, 1 1 Amamiya, Tsutsumi dori, Aobaku, Sendai 981 8555, Japan
    Appl Environ Microbiol 72:2407-13. 2006
    ..5 kDa) isolated from the culture broth of Aspergillus oryzae RIB40 grown in a medium containing polybutylene succinate-co-adipate (PBSA) as a sole carbon source...
  9. ncbi Aspergillus oryzae laeA regulates kojic acid synthesis genes
    Ken Oda
    Genome Biotechnology Laboratory, Kanazawa Institute of Technology
    Biosci Biotechnol Biochem 75:1832-4. 2011
    Kojic acid synthesis genes regulation was investigated in Aspergillus oryzae. Our results indicate that kojic acid production was lost in the laeA disruption strain, but was recovered in the LaeA complement strain...
  10. ncbi Polyol accumulation by Aspergillus oryzae at low water activity in solid-state fermentation
    George J G Ruijter
    Wageningen Centre for Food Sciences, PO Box 557, 6700AN Wageningen, The Netherlands
    Microbiology 150:1095-101. 2004
    Polyol accumulation and metabolism were examined in Aspergillus oryzae cultured on whole wheat grains or on wheat dough as a model for solid-state culture...
  11. doi A novel Aspergillus oryzae esterase that hydrolyzes 4-hydroxybenzoic acid esters
    Takuya Koseki
    Department of Bioresource Engineering, Faculty of Agriculture, Yamagata University, Tsuruoka, Japan
    FEBS Lett 584:4032-6. 2010
    In this study we report the biochemical characterization of a hypothetical protein from Aspergillus oryzae exhibiting sequence identity with feruloyl esterase and tannase from the genus Aspergillus...
  12. pmc Vacuolar membrane dynamics in the filamentous fungus Aspergillus oryzae
    Jun ya Shoji
    Department of Biotechnology, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 8657, Japan
    Eukaryot Cell 5:411-21. 2006
    ..Aovam3, the homologue of the Saccharomyces cerevisiae VAM3 gene that encodes the vacuolar syntaxin, from Aspergillus oryzae. In yeast complementation analyses, the expression of Aovam3 restored the phenotypes of both Deltavam3 and ..
  13. ncbi Cloning and characterization of a chitosanase gene from the koji mold Aspergillus oryzae strain IAM 2660
    X Y Zhang
    Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, Ueda, Nagano, Japan
    Biosci Biotechnol Biochem 65:977-81. 2001
    A genomic copy of the gene coding for chitosanase (csnA) was isolated from Aspergillus oryzae IAM 2660. A...
  14. doi Application of a statistical design to the optimization of parameters and culture medium for alpha-amylase production by Aspergillus oryzae CBS 819.72 grown on gruel (wheat grinding by-product)
    Radhouane Kammoun
    Centre de Biotechnologie de Sfax, Laboratoire d Enzymes et Métabolites des Procaryotes, Route de Sidi Mansour Km 4, BP K 3038 Sfax, Tunisia
    Bioresour Technol 99:5602-9. 2008
    The production optimization of alpha-amylase (E.C.3.2.1.1) from Aspergillus oryzae CBS 819...
  15. pmc Molecular analysis of an inactive aflatoxin biosynthesis gene cluster in Aspergillus oryzae RIB strains
    Mihoko Tominaga
    National Research Institute of Brewing, 3 7 1 Kagamiyama, Higashi Hiroshima 739 0046, Japan
    Appl Environ Microbiol 72:484-90. 2006
    To help assess the potential for aflatoxin production by Aspergillus oryzae, the structure of an aflatoxin biosynthesis gene homolog cluster in A. oryzae RIB 40 was analyzed...
  16. ncbi Evidence of RIP (repeat-induced point mutation) in transposase sequences of Aspergillus oryzae
    Maria D Montiel
    Institute of Genetics, School of Biology, University of Nottingham, University Park, Nottingham NG7 2RD, UK
    Fungal Genet Biol 43:439-45. 2006
    ..awamori, and was found to be present in at least 20 copies in the Aspergillus oryzae database. Analysis of four copies showed evidence of C:G to T:A transitions in at least 98...
  17. ncbi Dependence of morphology on agitation intensity in fed-batch cultures of Aspergillus oryzae and its implications for recombinant protein production
    A Amanullah
    Centre for Bioprocess Engineering, School of Chemical Engineering, The University of Birmingham, Edgbaston, Birmingham B 15 2TT, UK
    Biotechnol Bioeng 77:815-26. 2002
    ..such changes did not lead to different levels of recombinant protein production in chemostat cultures of Aspergillus oryzae (Amanullah et al., 1999)...
  18. doi Characterization of two distinct feruloyl esterases, AoFaeB and AoFaeC, from Aspergillus oryzae
    Takuya Koseki
    Department of Bioresource Engineering, Yamagata University, Tsuruoka, Japan
    Appl Microbiol Biotechnol 83:689-96. 2009
    ..showing sequence identity with known type-C feruloyl esterases, have been found in the genomic sequence of Aspergillus oryzae. We cloned the putative A. oryzae feruloyl esterase-encoding genes and expressed them in Pichia pastoris...
  19. pmc Metabolic engineering of the morphology of Aspergillus oryzae by altering chitin synthesis
    Christian Muller
    Center for Process Biotechnology, BioCentrum DTU, Technical University of Denmark, 2800 Kgs Lyngby
    Appl Environ Microbiol 68:1827-36. 2002
    ..production during submerged cultivation were examined in a wild-type strain (A1560) and in strains of Aspergillus oryzae in which chitin synthase B (chsB) and chitin synthesis myosin A (csmA) have been disrupted (ChsB/G and CM101)..
  20. doi Identification and characterization of genes responsible for biosynthesis of kojic acid, an industrially important compound from Aspergillus oryzae
    Yasunobu Terabayashi
    Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology, Higashi 1 1 1, Tsukuba, Ibaraki, Japan
    Fungal Genet Biol 47:953-61. 2010
    Kojic acid is produced in large amounts by Aspergillus oryzae as a secondary metabolite and is widely used in the cosmetic industry...
  21. pmc Macroautophagy-mediated degradation of whole nuclei in the filamentous fungus Aspergillus oryzae
    Jun ya Shoji
    Department of Biotechnology, The University of Tokyo, Tokyo, Japan
    PLoS ONE 5:e15650. 2010
    ..Here, we show that autophagy mediates degradation of basal cell components in the filamentous fungus Aspergillus oryzae. In basal cells, enhanced green fluorescent protein (EGFP)-labeled peroxisomes, mitochondria, and even ..
  22. doi Multiple gene disruptions by marker recycling with highly efficient gene-targeting background (DeltaligD) in Aspergillus oryzae
    Jun ichi Maruyama
    Department of Biotechnology, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 8657, Japan
    Biotechnol Lett 30:1811-7. 2008
    ..that double disruption of the proteinase genes (tppA and pepE) improved heterologous protein production by Aspergillus oryzae (Jin et al. Appl Microbiol Biotechnol 76:1059-1068, 2007). Since A...
  23. doi Analysis of autophagy in Aspergillus oryzae by disruption of Aoatg13, Aoatg4, and Aoatg15 genes
    Takashi Kikuma
    Department of Biotechnology, The University of Tokyo, Bunkyo ku, Tokyo, Japan
    FEMS Microbiol Lett 316:61-9. 2011
    ..To further understand autophagy in the filamentous fungus Aspergillus oryzae, we first constructed A...
  24. ncbi Branching mutants of Aspergillus oryzae with improved amylase and protease production on solid substrates
    R te Biesebeke
    Wageningen Centre for Food Sciences, P O Box 557, 6700 AN, Wageningen, The Netherlands
    Appl Microbiol Biotechnol 69:44-50. 2005
    ..tips and protein secretion during growth on a solid substrate, we have constructed two mutant strains of Aspergillus oryzae with increased hyphal branching...
  25. ncbi Phylogeny of fungal hemoglobins and expression analysis of the Aspergillus oryzae flavohemoglobin gene fhbA during hyphal growth
    Rob te Biesebeke
    Top Institute Food and Nutrition, P O Box 557, 6700 AN Wageningen, The Netherlands
    Fungal Biol 114:135-43. 2010
    The fhbA genes encoding putative flavohemoglobins (FHb) from Aspergillus niger and Aspergillus oryzae were isolated. Comparison of the deduced amino acid sequence of the A...
  26. ncbi Square-plate culture method allows detection of differential gene expression and screening of novel, region-specific genes in Aspergillus oryzae
    Kumiko Masai
    Graduate School of Agricultural and Life Sciences, Department of Biotechnology, University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo, 113 8657, Japan
    Appl Microbiol Biotechnol 71:881-91. 2006
    When grown on solid agar medium, the mycelium of a filamentous fungus, Aspergillus oryzae, forms three morphologically distinct regions: the tip (T), white (W), and basal (B) regions...
  27. doi Functional analysis and subcellular location of two flavohemoglobins from Aspergillus oryzae
    Shengmin Zhou
    Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 8657, Japan
    Fungal Genet Biol 48:200-7. 2011
    ..two distinct types of FHbs (predictive cytosolic FHb1 and predictive mitochondrial FHb2) from the fungus Aspergillus oryzae were investigated to elucidate the physiological roles of these FHbs...
  28. doi Construction of quintuple protease gene disruptant for heterologous protein production in Aspergillus oryzae
    Jaewoo Yoon
    Department of Biotechnology, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 8657, Japan
    Appl Microbiol Biotechnol 82:691-701. 2009
    b>Aspergillus oryzae has received attention as a host for heterologous protein production. However, A...
  29. ncbi Pyrithiamine resistance gene (ptrA) of Aspergillus oryzae: cloning, characterization and application as a dominant selectable marker for transformation
    T Kubodera
    Research and Development Department, Hakutsuru Sake Brewing Co Ltd, Kobe, Japan
    Biosci Biotechnol Biochem 64:1416-21. 2000
    ..PT) resistance gene (ptrA) was cloned from a genomic DNA library prepared from a PT resistant mutant of Aspergillus oryzae. It conferred high resistance to PT on an A. oryzae industrial strain as well as A. nidulans...
  30. doi Aspergillus oryzae atfA controls conidial germination and stress tolerance
    Kazutoshi Sakamoto
    National Research Institute of Brewing, 3 7 1 Kagamiyama, Higashi Hiroshima, Hiroshima, Japan
    Fungal Genet Biol 46:887-97. 2009
    We compared atfA and atfB, the genes encoding the respective ATF/CREB-type transcription factors in Aspergillus oryzae. The germination ratio of DeltaatfA conidia was low without any stress, unlike that of DeltaatfB conidia...
  31. pmc Proteomic analysis of extracellular proteins from Aspergillus oryzae grown under submerged and solid-state culture conditions
    Ken Oda
    National Research Institute of Brewing, 3 7 1 Kagamiyama, Higashi Hiroshima, Hiroshima 739 0046, Japan
    Appl Environ Microbiol 72:3448-57. 2006
    ..Recently, there has been increasing interest in Aspergillus oryzae because of its ability to produce heterologous proteins in solid-state culture...
  32. pmc Improved annotation through genome-scale metabolic modeling of Aspergillus oryzae
    Wanwipa Vongsangnak
    Department of Systems Biology, Technical University of Denmark, DK 2800 Lyngby, Denmark
    BMC Genomics 9:245. 2008
    Since ancient times the filamentous fungus Aspergillus oryzae has been used in the fermentation industry for the production of fermented sauces and the production of industrial enzymes. Recently, the genome sequence of A...
  33. pmc Endocytosis is crucial for cell polarity and apical membrane recycling in the filamentous fungus Aspergillus oryzae
    Yujiro Higuchi
    Department of Biotechnology, The University of Tokyo, Yayoi, Bunkyo ku, Tokyo, Japan
    Eukaryot Cell 8:37-46. 2009
    ..dibromide (FM4-64) and the plasma membrane protein AoUapC (Aspergillus oryzae UapC) fused to enhanced green fluorescent protein (EGFP) were internalized from the plasma membrane by ..
  34. doi In vivo imaging of endoplasmic reticulum and distribution of mutant α-amylase in Aspergillus oryzae
    Shinya Kimura
    Department of Biotechnology, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo, Japan
    Fungal Genet Biol 47:1044-54. 2010
    ..by expression of AoClxA and AoSec13 fused with fluorescent protein, respectively, in the filamentous fungus Aspergillus oryzae. Discrete tER subdomains were visualized as the punctate dots of AoSec13 overlapping or associated with ..
  35. ncbi Transcriptional activator, AoXlnR, mediates cellulose-inductive expression of the xylanolytic and cellulolytic genes in Aspergillus oryzae
    Junichiro Marui
    Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University, Japan
    FEBS Lett 528:279-82. 2002
    AoXlnR was isolated as a transcriptional activator of the major xylanase gene, xynF1, in Aspergillus oryzae. To investigate the spectrum of genes under the control of AoXlnR, expression of the xylanolytic and cellulolytic genes in an A...
  36. ncbi Nuclear translocation of the heterotrimeric CCAAT binding factor of Aspergillus oryzae is dependent on two redundant localising signals in a single subunit
    Hideya Goda
    Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, 464 8601 Japan
    Arch Microbiol 184:93-100. 2005
    ..nidulans. The nuclear translocation of the A. oryzae complex was found to be dependent on two redundant localising signals in HapB...
  37. ncbi Double disruption of the proteinase genes, tppA and pepE, increases the production level of human lysozyme by Aspergillus oryzae
    Feng Jie Jin
    Department of Biotechnology, The University of Tokyo, 1 1 1 Yayoi, Tokyo 113 8657, Japan
    Appl Microbiol Biotechnol 76:1059-68. 2007
    ..In this study, we investigated the effects of proteinase gene disruption on heterologous protein production by Aspergillus oryzae. The human lysozyme (HLY) was selected for recombinant production as a model for the heterologous protein...
  38. doi Discovery of a new tyrosinase-like enzyme family lacking a C-terminally processed domain: production and characterization of an Aspergillus oryzae catechol oxidase
    Chiara Gasparetti
    VTT Technical Research Centre of Finland, P O Box 1000, Espoo, 02044 VTT, Finland
    Appl Microbiol Biotechnol 86:213-26. 2010
    ..Among them, a sequence from Aspergillus oryzae (408 aa, AoCO4) was selected for production and characterization...
  39. doi Septum-directed secretion in the filamentous fungus Aspergillus oryzae
    Yugo Hayakawa
    Department of Biotechnology, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 8657, Japan
    Mol Microbiol 81:40-55. 2011
    ..dynamics of EGFP-fused α-amylase (AmyB-EGFP), the representative secretory enzyme of the filamentous fungus Aspergillus oryzae. We found that AmyB-EGFP accumulates in Spitzenkörper at hyphal tips as well as septal periplasm between ..
  40. doi Aspergillus oryzae flavohemoglobins promote oxidative damage by hydrogen peroxide
    Shengmin Zhou
    Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo ku, Tokyo 113 8657, Japan
    Biochem Biophys Res Commun 394:558-61. 2010
    ..Here, we investigate the relationship between two FHbs in Aspergillus oryzae (cytosolic FHb1 and mitochondrial FHb2) and oxidative stress...
  41. doi Secretory expression of the non-secretory-type Lentinula edodes laccase by Aspergillus oryzae
    Akira Yano
    Iwate Biotechnology Research Center, 22 174 4 Narita, Kitakami, Iwate 024 0003, Japan
    Microbiol Res 164:642-9. 2009
    ..Here, we report the production of Lcc1 and Lcc4 by Aspergillus oryzae and the extracellular secretory production of Lcc4 using a modified secretion signal peptide (SP) from Lcc1...
  42. ncbi Development of recombinant Aspergillus oryzae whole-cell biocatalyst expressing lipase-encoding gene from Candida antarctica
    Sriappareddy Tamalampudi
    Department of Molecular Science and Material Engineering, Graduate School of Science and Technology, Kobe University, 1 1 Rokkodaicho, Nada Ku, Kobe, 657 8501, Japan
    Appl Microbiol Biotechnol 75:387-95. 2007
    To expand the industrial applications of Candida antarctica lipase B (CALB), we developed Aspergillus oryzae whole-cell biocatalyst expressing the lipase-encoding gene from C. antarctica. A...
  43. ncbi Degradation of raffinose oligosaccharides in soymilk by immobilized alpha-galactosidase of Aspergillus oryzae
    Girigowda Kotiguda
    Department of Biochemistry, Gulbarga University, Gulbarga 585106, Karnataka, India
    J Microbiol Biotechnol 17:1430-6. 2007
    ..Therefore, the present study demonstrated that immobilized alpha-galactosidase in a continuous mode is efficient for reducing the oligosaccharides present in soymilk, which may be of considerable interest for industrial application...
  44. ncbi Microbial production of sensory-active miraculin
    Keisuke Ito
    Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 8657, Japan
    Biochem Biophys Res Commun 360:407-11. 2007
    ..amount of MCL to examine the mechanism involved in this sensory event at the molecular level, we transformed Aspergillus oryzae by introducing the MCL gene...
  45. ncbi Telomeric repeat sequence of Aspergillus oryzae consists of dodeca-nucleotides
    K I Kusumoto
    National Food Research Institute, 2 1 12 Kannondai, Tsukuba, Ibaraki, 305 8642, Japan
    Appl Microbiol Biotechnol 61:247-51. 2003
    Four telomeres in the chromosomes of Aspergillus oryzae NFRI1599 were cloned and sequenced. The telomeric repeat sequence of A. oryzae consisted of dodeca-nucleotides: TTAGGGTCAACA...
  46. doi Loss of Aspergillus oryzae amyR function indirectly affects hemicellulolytic and cellulolytic enzyme production
    Jun Watanabe
    Manufacturing Division, Yamasa Corporation, Choushi, Chiba 288 0056, Japan
    J Biosci Bioeng 111:408-13. 2011
    b>Aspergillus oryzae AB390, a derivative of A. oryzae OR101, was found to be suitable for soy sauce production, yielding a product light brown in color...
  47. ncbi Pulsed addition of limiting-carbon during Aspergillus oryzae fermentation leads to improved productivity of a recombinant enzyme
    Swapnil Bhargava
    Department of Chemical and Biochemical Engineering, University of Maryland, Baltimore County, 1000 Hilltop Circle, Baltimore, Maryland 21250, USA
    Biotechnol Bioeng 82:111-7. 2003
    ..As a control, three Aspergillus oryzae fed-batch fermentations were carried out with continuous addition of limiting-carbon...
  48. pmc Presence and functionality of mating type genes in the supposedly asexual filamentous fungus Aspergillus oryzae
    Ryuta Wada
    Department of Biotechnology, The University of Tokyo, Tokyo, Japana Bio c Co, Ltd, Toyohashi, Japan
    Appl Environ Microbiol 78:2819-29. 2012
    The potential for sexual reproduction in Aspergillus oryzae was assessed by investigating the presence and functionality of MAT genes. Previous genome studies had identified a MAT1-1 gene in the reference strain RIB40...
  49. doi Synthesis of galacto-oligosaccharides by β-galactosidase from Aspergillus oryzae using partially dissolved and supersaturated solution of lactose
    Carlos Vera
    School of Biochemical Engineering, Pontificia Universidad Catolica de Valparaiso, Avenida Brasil 2147, Valparaiso, Chile
    Enzyme Microb Technol 50:188-94. 2012
    ..temperature has been studied for the kinetically controlled reaction of lactose transgalactosylation with Aspergillus oryzae β-galactosidase, to produce prebiotic galacto-oligosaccharides (GOS)...
  50. doi Kojic acid biosynthesis in Aspergillus oryzae is regulated by a Zn(II)(2)Cys(6) transcriptional activator and induced by kojic acid at the transcriptional level
    Junichiro Marui
    National Institute of Advanced Industrial Science and Technology, AIST Tsukuba Central 6, Tsukuba, Ibaraki, Japan
    J Biosci Bioeng 112:40-3. 2011
    ..factor is clustered with two genes involved in biosynthesis of a secondary metabolite, kojic acid (KA), in Aspergillus oryzae. We determined that the gene was essential for KA production and the transcriptional activation of KA ..
  51. pmc Comparative genome analysis between Aspergillus oryzae strains reveals close relationship between sites of mutation localization and regions of highly divergent genes among Aspergillus species
    Myco Umemura
    Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology AIST, Higashi Nijo 17 2 1, Tsukisamu, Sapporo, Hokkaido 062 8517, Japan
    DNA Res 19:375-82. 2012
    b>Aspergillus oryzae has been utilized for over 1000 years in Japan for the production of various traditional foods, and a large number of A...
  52. ncbi Using a new GPI-anchored-protein identification system to mine the protein databases of Aspergillus fumigatus, Aspergillus nidulans, and Aspergillus oryzae
    Wei Cao
    Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo ku, Tokyo 113 8657, Japan
    J Gen Appl Microbiol 55:381-93. 2009
    ..we identified 115 GPI-anchored proteins in Aspergillus fumigatus, 129 in Aspergillus nidulans, and 136 in Aspergillus oryzae. Sequence-based conserved domain search found nearly half of these proteins to have conserved domains that ..
  53. doi Purification and properties of a novel phenolic antioxidant from Radix astragali fermented by Aspergillus oryzae M29
    I Chuan Sheih
    Department of Food Science and Biotechnology, National Chung Hsing University, 250 Kuokuang Road, Taichung 40227, Taiwan, Republic of China
    J Agric Food Chem 59:6520-5. 2011
    ..These data demonstrated that the solid-state bioprocessing strategy could be an innovative approach to enhance the antioxidant activity of RA...
  54. ncbi Pulsed feeding during fed-batch Aspergillus oryzae fermentation leads to improved oxygen mass transfer
    Swapnil Bhargava
    Department of Chemical and Biochemical Engineering, University of Maryland, Baltimore County UMBC, 1000 Hilltop Circle, Baltimore, Maryland 21250, USA
    Biotechnol Prog 19:1091-4. 2003
    ..As a model, an industrially relevant recombinant strain of Aspergillus oryzae was grown in carbon-limited, fed-batch mode...
  55. doi Cultivation characteristics and gene expression profiles of Aspergillus oryzae by membrane-surface liquid culture, shaking-flask culture, and agar-plate culture
    Hiroyuki Imanaka
    Division of Chemistry and Biochemistry, Graduate School of Natural Science and Technology, Okayama University, 3 1 1 Tsushima naka, Kita ku, Okayama 700 8530, Japan
    J Biosci Bioeng 109:267-73. 2010
    We cultivated a filamentous fungus, Aspergillus oryzae IAM 2706 by three different cultivation methods, i.e...
  56. doi Genetic analysis of conidiation regulatory pathways in koji-mold Aspergillus oryzae
    Masahiro Ogawa
    Noda Institute for Scientific Research, Noda City, Chiba 278 0037, Japan
    Fungal Genet Biol 47:10-8. 2010
    Conidia of koji-mold Aspergillus oryzae are often used as starters in the fermented food industry. However, little is known about conidiation regulation in A. oryzae. To improve the productivity of conidia in A...
  57. doi Heterologous expression and biochemical characterization of novel pyranose 2-oxidases from the ascomycetes Aspergillus nidulans and Aspergillus oryzae
    Ines Pisanelli
    Food Biotechnology Lab, Department of Food Sciences and Technology, BOKU University of Natural Resources and Life Sciences, Vienna, Austria
    Appl Microbiol Biotechnol 93:1157-66. 2012
    ..A putative pyranose 2-oxidase-encoding gene was also identified in the genome of Aspergillus oryzae. The coding sequence was synthetically produced and was also expressed in E. coli...
  58. doi Toxicological studies on a novel phytase expressed from synthetic genes in Aspergillus oryzae
    J Lichtenberg
    Novozymes A S, Toxicology, Bagsvaerd, Denmark
    Regul Toxicol Pharmacol 60:401-10. 2011
    ..The current study presents a safety evaluation of a 6-phytase produced by an Aspergillus oryzae strain expressing two synthetic genes, both mimicking a phytase gene from a Citrobacter braakii strain...
  59. doi Disruption of ten protease genes in the filamentous fungus Aspergillus oryzae highly improves production of heterologous proteins
    Jaewoo Yoon
    Department of Biotechnology, The University of Tokyo, Bunkyo ku, Japan
    Appl Microbiol Biotechnol 89:747-59. 2011
    ..of the significant problems to be solved in heterologous protein production by filamentous fungi including Aspergillus oryzae. Double (tppA, and pepE) and quintuple (tppA, pepE, nptB, dppIV, and dppV) disruption of protease genes ..
  60. pmc Enhanced production and secretion of heterologous proteins by the filamentous fungus Aspergillus oryzae via disruption of vacuolar protein sorting receptor gene Aovps10
    Jaewoo Yoon
    Department of Biotechnology, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 8657, Japan
    Appl Environ Microbiol 76:5718-27. 2010
    ..In this study, cDNA encoding AoVps10 from the filamentous fungus Aspergillus oryzae was cloned and sequenced...
  61. pmc The evolutionary imprint of domestication on genome variation and function of the filamentous fungus Aspergillus oryzae
    John G Gibbons
    Department of Biological Sciences, Vanderbilt University, Nashville, TN 37235, USA
    Curr Biol 22:1403-9. 2012
    ..we systematically examined genome-wide sequence and functional variation between the domesticated fungus Aspergillus oryzae, whose saccharification abilities humans have harnessed for thousands of years to produce sake, soy sauce, ..
  62. doi Efficient production and partial characterization of aspartyl aminopeptidase from Aspergillus oryzae
    K I Kusumoto
    National Food Research Institute, Tsukuba, Ibaraki, Japan
    J Appl Microbiol 105:1711-9. 2008
    ..Therefore, attention is focused here on the efficient production of this enzyme by a recombinant Aspergillus oryzae and characterization of its biochemical properties.
  63. ncbi Translation efficiency mediated by the 5' untranslated region greatly affects protein production in Aspergillus oryzae
    Akio Koda
    General Research Laboratory, Ozeki Corporation, 4 9, Imazu, Dezaike cho, Nishinomiya shi, Hyogo, 663 8227, Japan
    Appl Microbiol Biotechnol 66:291-6. 2004
    ..that the 5' untranslated region (5'UTR) plays an important role in determining translation efficiency in Aspergillus oryzae, using a model beta-glucuronidase (GUS) expression system...
  64. ncbi 5' Untranslated region of the Hsp12 gene contributes to efficient translation in Aspergillus oryzae
    Akio Koda
    General Research Laboratory, Ozeki Corp, 4 9, Imazu, Dezaike cho, Nishinomiya shi, Hyogo, 663 8227, Japan
    Appl Microbiol Biotechnol 70:333-6. 2006
    ..a 5' untranslated region (5'UTR) that dramatically increases the expression level of an exogenous gene in Aspergillus oryzae. Using a series of 5'UTR::GUS (uidA) fusion constructs, we analyzed the translation efficiency of chimeric ..
  65. ncbi Genomics reveals traces of fungal phenylpropanoid-flavonoid metabolic pathway in the f ilamentous fungus Aspergillus oryzae
    Praveen Rao Juvvadi
    Department of Biotechnology, The University of Tokyo, Bunkyo ku, Japan
    J Microbiol 43:475-86. 2005
    ..chalcone synthase (CHS) genes belonging to the type III PKS superfamily in an industrially important fungus, Aspergillus oryzae. CHS genes are known to play a vital role in the biosynthesis of flavonoids in plants...
  66. doi A novel enzymatic microreactor with Aspergillus oryzae β-galactosidase immobilized on silicon dioxide nanosprings
    Karl F Schilke
    School of Chemical, Biological and Environmental Engineering, Oregon State University, Corvallis, OR 97331, USA
    Biotechnol Prog 26:1597-605. 2010
    ..SPDP-modified β-galactosidase from Aspergillus oryzae was immobilized on the thiolated nanosprings by reversible disulfide linkages...
  67. doi Acid protease production by solid-state fermentation using Aspergillus oryzae MTCC 5341: optimization of process parameters
    K S Vishwanatha
    Department of Protein Chemistry and Technology, Central Food Technological Research Institute, Mysore, India
    J Ind Microbiol Biotechnol 37:129-38. 2010
    b>Aspergillus oryzae MTCC 5341, when grown on wheat bran as substrate, produces several extracellular acid proteases. Production of the major acid protease (constituting 34% of the total) by solid-state fermentation is optimized...
  68. ncbi Sequence analysis and overexpression of a pectin lyase gene (pel1) from Aspergillus oryzae KBN616
    N Kitamoto
    Food Research Institute, Aichi Prefectural Government, Nagoya, Japan
    Biosci Biotechnol Biochem 65:209-12. 2001
    A gene (pel1) encoding pectin lyase (Pel1) was isolated from a shoyu koji mold, Aspergillus oryzae KBN616, and characterized. The structural gene comprised 1,196 bp with a single intron...
  69. doi Heterologous expression and characterization of a glucose-stimulated β-glucosidase from the termite Neotermes koshunensis in Aspergillus oryzae
    Cristiane Akemi Uchima
    Department of Biotechnology, University of Tokyo, Tokyo, Japan
    Appl Microbiol Biotechnol 89:1761-71. 2011
    ..This β-glucosidase (G1NkBG) was successfully expressed in Aspergillus oryzae. G1NkBG was purified to homogeneity from the culture supernatant through ammonium sulfate precipitation and ..
  70. doi Identification of three mutant loci conferring carboxin-resistance and development of a novel transformation system in Aspergillus oryzae
    Yoko Shima
    National Food Research Institute, National Agriculture and Food Research Organization NARO, 2 1 12 Kannondai, Tsukuba, Ibaraki 305 8642, Japan
    Fungal Genet Biol 46:67-76. 2009
    Mutants exhibiting resistance to the fungicide, carboxin, were isolated from Aspergillus oryzae, and the mutations in the three gene loci, which encode succinate dehydrogenase (SDH) B, C, and D subunits, were identified to be ..
  71. doi Aspergillus oryzae lectin induces anaphylactoid oedema and mast cell activation through its interaction with fucose of mast cell-bound non-specific IgE
    K Yamaki
    Department of Pharmacology, Kobe Pharmaceutical University, Higashinada ku, Kobe, Hyogo, Japan
    Scand J Immunol 74:445-53. 2011
    We investigated whether Aspergillus oryzae lectin (AOL), a fucose-specific lectin, induces anaphylactoid reactions and mast cell activation. The injection of AOL into footpads of mice produced a dose-related acute paw oedema...
  72. ncbi Development of Aspergillus oryzae thiA promoter as a tool for molecular biological studies
    Jun ya Shoji
    Department of Biotechnology, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 8657, Japan
    FEMS Microbiol Lett 244:41-6. 2005
    ..Here, we report the development and application of the thiamine-regulatable thiA promoter (PthiA) in Aspergillus oryzae as a tool for molecular biological studies...
  73. ncbi Identification of growth phenotype-related genes in Aspergillus oryzae by heterologous macroarray and suppression subtractive hybridization
    R te Biesebeke
    TNO Quality of Life, Utrechtseweg 48, 3700 AJ, Zeist, The Netherlands
    Mol Genet Genomics 273:33-42. 2005
    b>Aspergillus oryzae requires polarized growth for colonization of solid substrates, and this growth phenotype differs from that seen in liquid medium...
  74. ncbi Aspergillus oryzae GB-107 fermentation improves nutritional quality of food soybeans and feed soybean meals
    Kee Jong Hong
    Department of Pathology, Texas Tech University Health Sciences Center, Texas Tech University, Lubbock, TX 79409, USA
    J Med Food 7:430-5. 2004
    ..Soybeans and soybean meals were fermented by Aspergillus oryzae GB-107 in a bed-packed solid fermentor for 48 hours...
  75. doi Simple metabolite extraction method for metabolic profiling of the solid-state fermentation of Aspergillus oryzae
    Masafumi Tokuoka
    National Research Institute of Brewing, 3 7 1 Kagamiyama, Higashihiroshima 739 0046, Japan
    J Biosci Bioeng 110:665-9. 2010
    ..here a simple method for metabolite extraction from the solid-state fermentation of a filamentous fungus, Aspergillus oryzae, which is known as rice-koji...
  76. ncbi Xylose triggers reversible phosphorylation of XlnR, the fungal transcriptional activator of xylanolytic and cellulolytic genes in Aspergillus oryzae
    Yuji Noguchi
    Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University, Aichi, Japan
    Biosci Biotechnol Biochem 75:953-9. 2011
    ..In order to clarify the molecular mechanisms underlying XlnR-mediated induction, Aspergillus oryzae XlnR was fused with the c-myc tag and examined by Western blotting...
  77. doi Effect of cultivation pH and agitation rate on growth and xylanase production by Aspergillus oryzae in spent sulphite liquor
    Zawadi A Chipeta
    Department of Microbial, Biochemical and Food Biotechnology, University of the Free State, P O Box 339, 9300 Bloemfontein, South Africa
    J Ind Microbiol Biotechnol 35:587-94. 2008
    The effects of cultivation pH and agitation rate on growth and extracellular xylanase production by Aspergillus oryzae NRRL 3485 were investigated in bioreactor cultures using spent sulphite liquor (SSL) and oats spelts xylan as ..
  78. doi High-level expression and efficient purification of bioactive swollenin in Aspergillus oryzae
    Meihua Wang
    Institute of Bioengineering, Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou, China
    Appl Biochem Biotechnol 162:2027-36. 2010
    ..the productivity of swollenin, the effects of culture conditions on the expression level in recombinant Aspergillus oryzae were investigated systematically...
  79. doi A defect of LigD (human Lig4 homolog) for nonhomologous end joining significantly improves efficiency of gene-targeting in Aspergillus oryzae
    Osamu Mizutani
    Laboratory of Bioindustrial Genomics, Department of Bioindustrial Informatics and Genomics, Graduate School of Agricultural Science, Tohoku University, 1 1 Tsutsumidori Amamiyamachi, Aoba ku, Sendai 981 8555, Japan
    Fungal Genet Biol 45:878-89. 2008
    ..recombination occurs rarely during transformation since nonhomologous recombination is predominant in Aspergillus oryzae. To develop a highly efficient gene-targeting system for A...
  80. ncbi Improvement of heterologous protein production in Aspergillus oryzae by RNA interference with alpha-amylase genes
    Takashi Nemoto
    Department of Biotechnology, The University of Tokyo, Tokyo, Japan
    Biosci Biotechnol Biochem 73:2370-3. 2009
    b>Aspergillus oryzae RIB40 has three alpha-amylase genes (amyA, amyB, and amyC), and secretes alpha-amylase abundantly...
  81. doi Characterization and expression analysis of a maltose-utilizing (MAL) cluster in Aspergillus oryzae
    Sachiko Hasegawa
    Laboratory of Bioindustrial Genomics, Department of Bioindustrial Informatics and Genomics, Graduate School of Agricultural Science, Tohoku University, Aoba ku, Sendai 981 8555, Japan
    Fungal Genet Biol 47:1-9. 2010
    ..such as maltose and maltotriose induce the production of amylolytic enzymes including alpha-amylase in Aspergillus oryzae. A transcriptional activator gene amyR, required for maltose induction of amylolytic enzymes, has been ..
  82. ncbi Some studies of alpha-amylase production using Aspergillus oryzae
    Z Esfahanibolandbalaie
    Biotechnology Center, Iranian Research Organization for Science and Technology, No 71, Forsat Street, Enghelab Ave, P O Box 15815 3538, Tehran 15819, Iran
    Pak J Biol Sci 11:2553-9. 2008
    The extracellular alpha-amylase production by Aspergillus oryzae was studied in submerged fermentation using an Adlof-Kuhner orbital shaker. The effect of initial pH values in the range of 4 to 7...
  83. doi Distinct enzymatic and cellular characteristics of two secretory phospholipases A2 in the filamentous fungus Aspergillus oryzae
    Tomoyuki Nakahama
    Department of Biotechnology, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 8657, Japan
    Fungal Genet Biol 47:318-31. 2010
    ..We analyzed here two sPLA(2)s, named sPlaA and sPlaB, of the filamentous ascomycete Aspergillus oryzae. sPlaA and sPlaB consist of 222 and 160 amino acids, respectively, and share the conserved Cys and catalytic ..
  84. pmc Extracellular production of neoculin, a sweet-tasting heterodimeric protein with taste-modifying activity, by Aspergillus oryzae
    Ken ichiro Nakajima
    Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 8657, Japan
    Appl Environ Microbiol 72:3716-23. 2006
    ..For the production of recombinant NCL (rNCL) by Aspergillus oryzae, alpha-amylase with a KEX2 cleavage site, -K-R-, was fused upstream of each of NAS and NBS and the resulting ..
  85. pmc Genomics of Aspergillus oryzae: learning from the history of Koji mold and exploration of its future
    Masayuki Machida
    Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology, Central 6, 1 1, Higashi, Tsukuba, Ibaraki, Japan
    DNA Res 15:173-83. 2008
    ..A filamentous fungus, Aspergillus oryzae, is the key organism in the production of all these traditional foods, and its solid-state cultivation (SSC) ..
  86. doi Aspergillus oryzae atfB encodes a transcription factor required for stress tolerance in conidia
    Kazutoshi Sakamoto
    National Research Institute of Brewing, 3 7 1 Kagamiyama, Higashihiroshima, Hiroshima, Japan
    Fungal Genet Biol 45:922-32. 2008
    Using an Aspergillus oryzae EST database, we identified a gene encoding a transcription factor (atfB), which is a member of the ATF/CREB family...
  87. ncbi Development of a novel quadruple auxotrophic host transformation system by argB gene disruption using adeA gene and exploiting adenine auxotrophy in Aspergillus oryzae
    Feng Jie Jin
    Department of Biotechnology, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 8657, Japan
    FEMS Microbiol Lett 239:79-85. 2004
    We previously designed a triple auxotrophic host-vector system in Aspergillus oryzae by isolating red-colored adenine auxotrophic mutants upon UV mutagenesis of a double auxotrophic host (niaD-sC-)...
  88. ncbi High-level secretory production of phospholipase A1 by Saccharomyces cerevisiae and Aspergillus oryzae
    Y Shiba
    Lead Discovery Research Laboratories, Sankyo Co Ltd, Iwaki, Fukushima, Japan
    Biosci Biotechnol Biochem 65:94-101. 2001
    ..The PLA1 gene, which had been cloned from Aspergillus oryzae, was expressed in Saccharomyces cerevisiae and A. oryzae...
  89. ncbi Substrate aggregation due to aerial hyphae during discontinuously mixed solid-state fermentation with Aspergillus oryzae: experiments and modeling
    M A I Schutyser
    Wageningen Centre for Food Sciences, P O Box 557, 6700 AN Wageningen, The Netherlands
    Biotechnol Bioeng 83:503-13. 2003
    ..We developed a novel experimental set-up to measure the tensile strength of hyphal bonds in SSF: Aspergillus oryzae was cultivated between two wheat-dough disks and the tensile strength of the aerial mycelium was measured ..
  90. ncbi Secretion, purification, and characterization of a recombinant Aspergillus oryzae tannase in Pichia pastoris
    Xiaofen Zhong
    The Open Laboratory for Marine Functional Genomics of State High Tech Development, Key Laboratory of Genetic Engineering of MOE, Department of Biochemistry, College of Life Sciences, Sun Yat Sen University, Guangzhou 510275, People s Republic of China
    Protein Expr Purif 36:165-9. 2004
    ..In the present work, a tannase from Aspergillus oryzae has been cloned and expressed in Pichia pastoris...
  91. pmc Molecular and enzymic properties of recombinant 1, 2-alpha-mannosidase from Aspergillus saitoi overexpressed in Aspergillus oryzae cells
    E Ichishima
    Department of Bioengineering, Faculty of Engineering, Soka University, Hachioji, Tokyo 192 8577, Japan
    Biochem J 339:589-97. 1999
    ..5 kbp) and thus the Aspergillus oryzae expression plasmid pNAN-AM1 (11.2 kbp) was constructed...
  92. ncbi Cloning of the Aspergillus oryzae 5-aminolevulinate synthase gene and its use as a selectable marker
    S L Elrod
    Novo Nordisk Biotech Inc, Davis, CA 95616, USA
    Curr Genet 38:291-8. 2000
    The hemA gene encoding 5-aminolevulinate synthase, the first enzyme in heme biosynthesis, was cloned from Aspergillus oryzae and evaluated as a selectable marker for the transformation of filamentous fungi...
  93. ncbi Identification of Delta12-fatty acid desaturase from arachidonic acid-producing mortierella fungus by heterologous expression in the yeast Saccharomyces cerevisiae and the fungus Aspergillus oryzae
    E Sakuradani
    Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Osaka, Japan
    Eur J Biochem 261:812-20. 1999
    ..1) to linoleic acid, by its expression in both the yeast Saccharomyces cerevisiae and the fungus Aspergillus oryzae. Analysis of the fatty acid composition of yeast and fungus transformants demonstrated that linoleic acid (..
  94. ncbi A transcriptional activator, AoXlnR, controls the expression of genes encoding xylanolytic enzymes in Aspergillus oryzae
    Junichiro Marui
    Department of Biological Mechanisms and Functions, Nagoya University, Nagoya, 464 8601, Japan
    Fungal Genet Biol 35:157-69. 2002
    By deletion across the promoter region of the xynF1 gene encoding the major Aspergillus oryzae xylanase, a 53-bp DNA fragment containing the XlnR binding sequence GGCTAAA as well as two similar sequences was shown to confer xylan ..
  95. ncbi Release of short and proline-rich antihypertensive peptides from casein hydrolysate with an Aspergillus oryzae protease
    S Mizuno
    R and D Center, Calpis Co, Ltd, 11 10, 5 Chome, Fuchinobe, Sagamihara, Kanagawa 229 0006, Japan
    J Dairy Sci 87:3183-8. 2004
    ..Among these enzymes, a protease isolated from Aspergillus oryzae showed the highest angiotensin-I converting enzyme inhibitory activity per peptide. The A...
  96. ncbi Development of a two-site enzyme-linked immunosorbent assay for alpha-amylase from Aspergillus oryzae based on monoclonal antibodies
    I Sander
    Department of Allergology Immunology, Berufsgenossenschaftliches Forschungsinstitut für Arbeitsmedizin, Ruhr Universitat Bochum, Germany
    J Immunol Methods 210:93-101. 1997
    A two-site monoclonal antibody ELISA was developed to quantify the allergen Asp o 2 (alpha-amylase from Aspergillus oryzae)...
  97. ncbi Genome sequencing and analysis of Aspergillus oryzae
    Masayuki Machida
    Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology AIST, Higashi 1 1 1, Tsukuba, Ibaraki 305 8566, Japan
    Nature 438:1157-61. 2005
    The genome of Aspergillus oryzae, a fungus important for the production of traditional fermented foods and beverages in Japan, has been sequenced...
  98. ncbi Cloning and characterization of the nagA gene that encodes beta-n-acetylglucosaminidase from Aspergillus nidulans and its expression in Aspergillus oryzae
    Sunhwa Kim
    Department of Biotechnology, University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 8657, Japan
    Biosci Biotechnol Biochem 66:2168-75. 2002
    ..b>Aspergillus oryzae strain carrying the nagA gene under the control of the improved glaA promoter produced large amounts of beta-..
  99. ncbi Visualization of nuclei in Aspergillus oryzae with EGFP and analysis of the number of nuclei in each conidium by FACS
    J Maruyama
    Department of Biotechnology, The University of Tokyo, Japan
    Biosci Biotechnol Biochem 65:1504-10. 2001
    b>Aspergillus oryzae has been reported to form conidia with multinuclei. In order to analyze nuclei in living cells, we developed an expression system of the A. nidutans histone H2B protein tagged by EGFP (H2B::EGFP). In both A...
  100. ncbi Molecular cloning and characterization of a transcriptional activator gene, amyR, involved in the amylolytic gene expression in Aspergillus oryzae
    K Gomi
    National Research Institute of Brewing, Hiroshima, Japan
    Biosci Biotechnol Biochem 64:816-27. 2000
    ..amyR, coding for a transcriptional activator involved in amylolytic gene expression has been cloned from Aspergillus oryzae by screening for a clone that enabled to reverse the reduced expression of the alpha-amylase gene (amyB) ..
  101. ncbi Cladal relatedness among Aspergillus oryzae isolates and Aspergillus flavus S and L morphotype isolates
    Perng Kuang Chang
    Southern Regional Research Center, U S Department of Agriculture, New Orleans, Louisiana 70124, USA
    Int J Food Microbiol 108:172-7. 2006
    ..flavus isolates. Our results show that A. flavus populations are genetically diverse. A. oryzae isolates may descend from certain atoxigenic L-type A. flavus isolates...